LOCUS       DAOQQV010000001        88721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_58_length_88721_cov_8.825908, whole genome shotgun sequence.
ACCESSION   DAOQQV010000001 DAOQQV010000000
VERSION     DAOQQV010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 88721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 88721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88721
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58_length_88721_cov_8.825908"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="PLL59_00005"
     CDS             complement(<1..1698)
                     /locus_tag="PLL59_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMY22211.1"
                     /translation="MKKTLLLWLFGLLLTTQVFAQNRSLSGTVKDATTGEALIGVNVT
                     GKGTTIGTVTDIDGKYTLELPKDVTTLVFSYIGYTNLEKPILSLVIDASMAVEGKQLE
                     ELVVTAMAIKRESRSITGSFNTVTSEDFNSGSTNAIGAIQSKVPGLRINTIGGQVGAS
                     TRIVLRGEASLTGGNNALIVVDGIPINNNTNSYGNSGSDFVDFGNRANDINPDDIESV
                     TVLKGPAATALYGSRGTAGVIIYTTKSGKGLKDNKKFNITASSGVTFDKVYLLMKRQD
                     QFGQGYDNGPDPIENFSWGPKFDGVVRPWTPAVSTPNGFSQLIRPYSAVKHQLEDAFN
                     TGTTVTSNLALEGGNDKFTYYVSYGNIRNKGIFPNTFYRRNNITANASAKLGDKVTTK
                     FNLAYSNINQRTIQGQALGGGFVGPYQILIQTPVNIPLSEVRDYNSIYQDFNGYYGGY
                     TPNPFWLMDNTNNDNKTDNVLGSVELEYKPVSYISLMARAGTNFTTSNSYISRPKYNY
                     AIGGANPTDADKYTEQVNRRNDITLDITAAFNKEIVKNVNLSVLAGYNFYDRKTNDIF
                     "
     gene            complement(1940..3064)
                     /gene="mqnC"
                     /locus_tag="PLL59_00010"
     CDS             complement(1940..3064)
                     /gene="mqnC"
                     /locus_tag="PLL59_00010"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HMY22212.1"
                     /translation="MTLSNLYQKALRFEFLSIEEGIFLYENAPTSELMAIGHQLRLIQ
                     KPDNIVTWQIDRNVNTTNVCVANCKFCNFYRKPGHQESYITDIDTYKTKIEETIRYGG
                     DQLLLQGGHHPELGLQFYKDTFSAIKKLFPHIKLHALGPPEIAHICKLEGMSHTEVLK
                     SLKEAGLDSLPGAGAEILNDRVRRLLAKGKCSGGEWLDVMRAAHQLHITTSATMMFGH
                     IETIYERMEHLVWIRQVQSEKPASAKGFLAFIPWPYQDEGTLLKKMRNISNQVTGDEY
                     IRMLAICRIMLPNIKNIQASWLTVGKDIAQMCLRAGANDFGSIMIEENVVSAAGAPHR
                     FTSKSIQQAILDAGFEPRLRNQQYEFRTLPEDVEVQVINY"
     gene            3222..4454
                     /locus_tag="PLL59_00015"
     CDS             3222..4454
                     /locus_tag="PLL59_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMY22213.1"
                     /translation="MIEYSKEVLKNGLTLIHHYDNSTPFVVVNTLYKIGAKHEEESRT
                     GFAHLFEHLMFSGSKHFPDFDKPLQEAGGENNAFTNNDYTNYYDTVPAINIEIPLCLE
                     ADRMSQLNINKKSLEVQRKVVCEEFKENYINQPYGNVWHLLREMVYTKHPYRWPTIGK
                     SLQHIEEATLNDVQNFYDTYYQPSNAVLVIAGNIEQANAKALTEKYFASIEGKPVSNK
                     QFEEPEQNVARKNTVYEEVPLNSIYIAFKMCDRLHPDYYVADVCSDVLSNGPSSRLHQ
                     RLVKEEKAFVEIDAYITASDDIGMFVFEGKISEHTSIETAEQLIWNEIEKIKQESVND
                     KELQKCKNKMLTYLSFSESNLLNRAIHLAAYEALGSIDLINAEEKNYDAVTAERISTF
                     AQHTFTKEKSNTLYYLKK"
     gene            4456..5715
                     /locus_tag="PLL59_00020"
     CDS             4456..5715
                     /locus_tag="PLL59_00020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMY22214.1"
                     /translation="MTKPTIHSIEKIHFPTPEHIVLSNAISLYGFNGAKNDILRIDVL
                     FNSGRWTEPAKLIAESTSKLFKSGTNNLSSFELNEQIDGFGTTIKASSGYNTFTVSLY
                     CLHRFLEPSLQLLRTCLTDIIFPENELEILQKNALSKLKISQEKNDYLADVAFKKTLF
                     GEDHPYGYETTETAIKNIHSALLKQFYATDITPSNCTVLIAGKYAAKELQLIEQYLGS
                     WQSSIQKQSPAQYQTLNVQQQCLRIKKENSVQASIVIGKESFNKHHKDYASFVLLNTI
                     FGGYFGSRLMSNIREEKGLTYGIYSGLSPLKYGGIFSIQTDTNLENVDLCLREIYAEM
                     ERLQTELISEQEIVLARNYLLGKFLSRTDGPFQQIEVFKSYFIEEVAINRFEEFVETI
                     RQTDALSLQRLAIQHLQKDSMNEIVVG"
     gene            5741..7561
                     /locus_tag="PLL59_00025"
     CDS             5741..7561
                     /locus_tag="PLL59_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY22215.1"
                     /translation="MNWRETILNKIVKHVFTAYCIFITAYSFSQTEFEAPKLTCVRNN
                     VGDIELQWQLPSSVNPCFVAYEIYASIGSKNGPYTLNTSITTASQTTALLTIASAGQP
                     VFFYMINRGSCNNPIPLAKKTSDTLDNIKPQPFVQLQNATVINNQVHLHWLAAASPEV
                     SAYLVYNDRDGFTSPDTVLGRLSTSYVDIVNDPSQFAIRYKIRALEYCEDVAGLQGSI
                     TPDSADHRTTLLQVGAADKCTQTANLSWQMYKIGSAQVVSYDIEQSINRGAFTTVGTQ
                     PGTSQSFLLQNIPFRDTICIRVKANLPNGTFAYSNERCLSADVIKRPENDYIRNITVE
                     HGDIIIEYHKDTTAAPARTVILQRSNDGIIFSPTLTVPTEPDPNTYLFTEPNLSVQNQ
                     TFTYRVNLLDSCFITHTSDTATTIRVGIKVKSNNKADIIWAGFEINNITFDHFLVEKI
                     IGTDTSAVGTFNRNQTTYAETALFDYTADSLQETCYRITAFFENNNDVAPRETLKSHS
                     NIVCVQPTPKVYVPQAFVPTGHNNTFKPFLLYAKADAYDFRIYDRWYQLMFATNDINE
                     SWDGTFKGVNAPLDGYIYIIKFKGKDDKDYTQTGTVMLLK"
     gene            7723..9411
                     /locus_tag="PLL59_00030"
     CDS             7723..9411
                     /locus_tag="PLL59_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22216.1"
                     /translation="MKYIKRIFHYYFLEPSNAVSVNRPAVIIGVLLLTIAGASVYYNI
                     YDYMDIVYGDEVIYMKNGLNILQDFNRDWGPLYCLWYKFLSLFIPDTITLYYFNFVLV
                     AILATVLIYCAFLSMDIHPLVAFYFAICFIGSKAVVPMWPRISLFTICILMIAIISIR
                     RIKALYARLMLVCIAVLLASYARPELYLSFLLSLFIFIAYFFAGKIYKKPYSIYHALV
                     FILLIVLLHLIFRFPSNFYNGYPRNLAAFYQHYMVNQFFLNKATEFDWIYWKDTHKAI
                     FSDANTNIFSIIIHYPIEFFSHVALNIKNYLVEIVVKNLSVIFPFLGGMNKLYFGGAF
                     LVFISSMLLLSISSIRQAFFITLKAHAFYLVMLLIWGIPTILSSIVIFPREHYIMLNY
                     FLFIIPLCFLFTEAAKQLLPNVSLKHFIIFAIVLIPFLSSSKNYHFFLTDHDKNNMCS
                     RKALQHIKNKHLDQHIQYTFFADIHCFIGFLPNNFKEINTIFDKTKDVSFATILMYNK
                     PDFILVNNCINYSPSLIDDTTWQQFIKNPYPFGYRYEFVKDCPYYYLVRNTVHD"
     gene            9404..10531
                     /locus_tag="PLL59_00035"
     CDS             9404..10531
                     /locus_tag="PLL59_00035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMY22217.1"
                     /translation="MIKKEFKHLDALRGIMAMYVTLVHFIILFTLNKKNELVFTDLRY
                     LGIVQLGQVFMTVFFVLSGFLISYILLIEKEQTANVNFKKFYLRRAFRILPMYYLGVF
                     SMFFLYKKACINPTIMQTPMCAELSERSCYYFLLLPNWAHAIDKTLPHISNYWSIGAE
                     EQFYILWPFVLYFSKNYLRSFFGIYIAYCCLLIAMVLVGNLYYHNDNITFINIAKLFD
                     YTRFGAFAFGGVIAFVLLHNTENTFQKYHLLMTKKSVQWSCFLLPFIVCIIPNEHVLF
                     LKHILVIPCCAVTVYNFAFDEQSILKIDNKYLNYIGKTTYSLYILNQIVIDFVIKICF
                     AAKINSTLIVFVITMFILFLGAVFCYEFIEKPFMQLRRKFG"
     gene            complement(10532..11458)
                     /locus_tag="PLL59_00040"
     CDS             complement(10532..11458)
                     /locus_tag="PLL59_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMY22218.1"
                     /translation="MKNKRTFYFIVGISILFVMLFINATTREDTYFPFIKAADKKENL
                     ALPLQNAYAVKLPESVSFAGENVPLDDIEVRERLDRELSVNAYWQSSTIIMLKRANRW
                     LPTIEKILKEESVPDDFKYLCMAESALDNAISSAGASGFWQFMKTTGLQYGLIINSDV
                     DERYNLEKSTRAACRYLRDAKEKTGSWTAAAAGYNMGLAGVNKQQLLQGETAYYDLFL
                     NTETSRYVQRIVALKIIHQHQKDYGFYLTNEDLYPELKYTIQQVSGPINWVSFAKENN
                     TSYKMLRIYNPWIRNFNLLNKERRTFDVKIPS"
     gene            complement(11520..12029)
                     /locus_tag="PLL59_00045"
     CDS             complement(11520..12029)
                     /locus_tag="PLL59_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HMY22219.1"
                     /translation="MADKLINKVAESGLISLDLETFFPNNPILEFDMKNYLFRELLLK
                     EKEFRTALQQQDWSIYKDKIVALHCSTDAILPQWSYMLVVSYLCAHTHEIYFGNTEKV
                     EYLLFIHNIKNMNIESYKDARVVIKGCGTKAIQGDAYIEISKKLQPVVKSLMFGEACS
                     TVPVYKKKN"
     gene            complement(12118..14289)
                     /locus_tag="PLL59_00050"
     CDS             complement(12118..14289)
                     /locus_tag="PLL59_00050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMY22220.1"
                     /translation="MALIGKIRQRSWILIGFIALALLIFIVEAALERNSLFSGGSGKN
                     SVGKINGSTISGKEYSERIANYEEGLKLINPNLQLNEQLQSQIQEEVWNTIAAQKLLG
                     DAYSSLGLAVSEGEMGELMWGDQPHPLAQRFLMQIRQVSPDVINQETGRLNQAKIREF
                     ITNIDKIDKDNKTNFRQMLSHIENLIKDDMVKQKYSSLVAQSFYMPTFMAKEVVNSGR
                     SAKVAFVSVPYASLSDDKYKVTDDEITAYLKANAAKFEQAASRVVDVVSFDIIPSAED
                     TAAAKAKILKMREEYIASLPNDSAFIARNSQQGAGVGYFSKDEVMQTNRSADTLFSLP
                     VGTLTNVYNEGSFLLFTKIIDRKSAPDSVRAAHILLSEGNGTEAEKKAANNLADSLIR
                     VIQSGAKNFGQVAVETSLDKDSKDKGGDLGYFTRNQMVKEFNDKVFYSGMAPGQIAKV
                     ETQYGLHIILLIDAKTPKLLTKFADFVVELAPSNETEKMAYDKAASFQQKYQTAEAFD
                     KAAKTENLAKNVVLTQNMVEVPQVGAARKLVQWAFQQEKVGVIADFDNDNKYMLAKLS
                     KIIPKGLPAAADVREEISILVRNEKKGKDLVAALDKAATGTTDLSTIAAKVKDAAVTD
                     TALVRMSSAFVQGLGNEPKLVGTTFGITVGKTSKAVAGERSAFMVQPKQIDANAPEMS
                     GDINMYKEQMQRMFISRMNFQTIFESILKKADVNDTRYIFY"
     gene            complement(14397..15665)
                     /locus_tag="PLL59_00055"
     CDS             complement(14397..15665)
                     /locus_tag="PLL59_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMY22221.1"
                     /translation="METSTILILIFLTLIFSAIFSGIEIAFLSANKLKIELNYQRGTL
                     SGKILNDYIKNPSKFIVTTLLGNNIALISFGILTAMLMEPILEEYLTNELLILSIQTF
                     VSTTIILFLGEFVPKVLFKVFADSILPLMALPFRIIHWILTPGVLLITGISTLLFKIF
                     GIKVSEQDIEFSSVDLEKFIKDHHQTNSEEEEEVDTELFENALYLKKLRVRDCMVPRR
                     EITAIDISDSVEELKQKIIESYHSRILIYEDSIDKIVGYIHHFDLHKKPSNIRSILMD
                     IKVVPETMHIQKLLNEFIKENKSIAWVVNEYGGTAGVITLEDILEEIFGDIQDEYDKD
                     ELVGNQLSKNEFILSGRLEIDRINEEYDLDLPEGDYDTLSGFIIDYHETIPEKNEIIE
                     IGKYSFKIIDVSETKIETVKLEINESADNQ"
     gene            complement(15874..16452)
                     /gene="lptC"
                     /locus_tag="PLL59_00060"
     CDS             complement(15874..16452)
                     /gene="lptC"
                     /locus_tag="PLL59_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HMY22222.1"
                     /translation="MKSAAKQPKYSIALLTLVAMLLIFSSCQENDIAKVKALFNETDA
                     DVEIADSVKFTYKEGAYSRAIVTGKTVKRFTNSKNRLEFTDGLLVKFYDKLNLISVMK
                     ANYAENNDEEQKVYIKGKVYIENSRYEILETEELTWDIRNKKIFTSKPIKIKTPDNTI
                     YGVGFDADEDFSNYTIRKVSGIVAVSNAQGFQ"
     gene            complement(16470..17792)
                     /locus_tag="PLL59_00065"
     CDS             complement(16470..17792)
                     /locus_tag="PLL59_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMY22223.1"
                     /translation="MTFLLSCFFYVNAQDKKATLEAKKKKLLDEIEFTQKVLTKTKAS
                     KDATLADLQALSKQIELRQKLIKEVEGEIGDFTEKINQNVDSLKQKESQLTKLKKEYA
                     DAIVRTYKSQRFADKLLFVVNANSFSEALRRVNYLRKYATFREQQATQIIDKKSDIST
                     QIANIDAKKKTKEQLLQGQVKEKVQLNKSVSQKNTVVQTLKKKEKELQTNITKKQSEA
                     KKLDAQIEAIIKKEIELARLAEEKKQKEAAAKLAAAEAAKKKAAEEAIKKAKEAGKEP
                     SKEDIATVNKKVTETPVKLGNTPEYDQLSSNFYGNKGKLPWPVEKGFISKGFGKYTVP
                     GSSIVMENNGIDIRTDANSSVRSAFDGKVVGILNNPTFKNAVIISHGEYFTVYSKLAS
                     VNVAKGQKISTKQVIGTAYTDEDNITEVHLEVWKGAAKLDPSNWIYKK"
     gene            complement(17857..18705)
                     /locus_tag="PLL59_00070"
     CDS             complement(17857..18705)
                     /locus_tag="PLL59_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HMY22224.1"
                     /translation="MANFSFYKIKITTLLFMLMSMLLLLPACHRNKKIKKTDEIVSVP
                     IKPIAPTPTIPTPIKIDAAVSSLLAKVNNNEIVFKELALKLKTKVTSPDLNQSFTTNI
                     RWKKNEKIWLSMSIIGIEGARVLITKDSIKIMDKINNRYVLKPLSYIKQKTLVDLSFA
                     DIENLLLGQLVFVDSTKAKYVENATNTTINADGVRFLTNVLFDKTSKNPTSIFVSDKK
                     YPQTIDVKYDNYQMQLGKPFSMDRELKMKNNTSTFEMIAKIQSIEVKQNMEYPFTINP
                     NYKIEK"
     gene            complement(18746..20521)
                     /locus_tag="PLL59_00075"
     CDS             complement(18746..20521)
                     /locus_tag="PLL59_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY22225.1"
                     /translation="MKNTLSIYLFSVVLLLSIAPSSFAKKNKKNKQDNSSSSTTVTTK
                     KDLELQALFIEGMQAYILGNSQDAITKFNEVLRRDAKNDAALYELSKIAFEAGNMDKS
                     IEFAQNAIKLNPNNEFYYQYLAEAKGEKKDFIGAAKVYENVIQLKPKEYDYYYDWAYM
                     LTKAEKYKDAIEVYDLLEQKTGVQEEVIFQKQPLYIKLGNIEDAVKDIEKLVKLYPSD
                     TRYIALAGELYLSNKMYEKALQTYNRIIAKEPNNADALLAIADIYKQKGDNKTHDEYL
                     IKVFNNPNIDIDTKIMAFIPYIERMAKDSSMRNEVMLMADKLVEAHPNDVKATTARAD
                     VLYNSNKKRDAQNEYVKAIHLGADIPNTVWIQLYILDTELEDYDKLIEHTKEGIQKNP
                     NDPFGYFYNAIAQQQKKNNKEASNAILQAFATQQKSTSNPIYTPQLKLQMLILLGDVS
                     FELKDYTRCDSSYEAALEIDPNNATVLNNYAYYLSERDDRLEKAERMSKKSNLLVDNN
                     SAFLDTYAWIMYKMKNYTEALTWIEQAMEFPDAQERAELLTHYGDILFKVGQVDKAVL
                     QWEKALQRGGDKIALELKIKNQKIN"
     gene            complement(20645..21079)
                     /gene="dut"
                     /locus_tag="PLL59_00080"
     CDS             complement(20645..21079)
                     /gene="dut"
                     /locus_tag="PLL59_00080"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HMY22226.1"
                     /translation="MKLKIVNTSDNPLPTYETEGSAGVDLRAQLQEPVMLKPLQRALI
                     PTGLFVELPDGYEAQVRPRSGLALKKGVTVLNSPGTIDSDYRGEIKIILINLSNETTI
                     INTGERIAQLIISKYEKVSFKEVDELNETKRGEGGFGHTGVK"
     gene            complement(21173..22669)
                     /locus_tag="PLL59_00085"
     CDS             complement(21173..22669)
                     /locus_tag="PLL59_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMY22227.1"
                     /translation="MYICPNPNNLSTVKKLASDTAAYGVSSILARLINFLFGFLIIKY
                     ITQAEFGTFTNFYAYAGFILVALTHGMETAYFRFANKAESKETAFTTAFFSVLIAVVL
                     FVILAYVFQNSIAEYVKEPKLYIQFFVWMMAFDVLAAIPFASLRKQGRPIAFAALKIL
                     NIALFIAFNIFFFLLWPILETKGISSTLMSSVPSKVSFVFIANILASILTFLFLIKQF
                     QQLQSRIDFALYKKMLGYAMPIMLVGFAGMVNEVLDRIIMTRLLPYDDITNKEQLGIY
                     GFNYKFAMLVSMFLQAYRYAAEPIFFRYADKSDNKQMLAKTMKYYTICVCVIFLSIVL
                     FLPLIQALFVLYSPNSINYFVGADIIPILLMANIFLGIYFNISTWYKITDKTYIGAII
                     SIIGASITIIANVILVPQIGYHGAAWSTLLCYLSMVLIAYIAEQKYYPIPYDYKRIVS
                     YIILALALYLLLKFLVLPMSFSLFLNTTIAVLLLLFYLFIAYFLEKKK"
     gene            22750..24606
                     /locus_tag="PLL59_00090"
     CDS             22750..24606
                     /locus_tag="PLL59_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22228.1"
                     /translation="MKSRIAFILIVIVYFTSFFYYKSYRNFLQGGGDPWGYYAYLPAL
                     FIYDDLHDLQKTIGKRAEYNASSVRTLENAYLQIEEAHAFKHNTIIKYTNGVAILYSP
                     FFFAAHFFCKSTNLYAADGYTLPYNIAVGFATIFYSLFGLWILRKLLSKYFSDSISAI
                     VLMSIAIGTNLYFFSVSHLGMSHVFLFALYALCLWATDCYYHSFKTKYAIAIGLACGM
                     ITLIRPNEIIILLFPFLWNVYSIDTFRERIETIRTHVGQYVLMTLLFLIVLLPQFLYW
                     KILSGNFIFYSYTKEGFDFLHPHIKEGILGFANGWLSYTPILFFAVIGLFFLHRNFKN
                     SALPSFLFLPIYIWVIYSWWCWQYINGFGSRPMIETYPIWAFPMAVFFTVIGKKIGLK
                     TLSLALVCFFIFLNIFQTWQFKAGLLWTEDSNFAHYRASFLQSKGSYNAHIAFDSREY
                     QPDSNKLQFVKTIQTQSFDDSFSNSFNATIYKSSPYAFELKEGTTPAIQIPANTAGVQ
                     ALDYFKISCWVYCPALQYDHYKQAVIAAEFVHNGAQIRWRPVRLQTKIDNPNHHLLHA
                     GKTKQWQYISFFVRAPHRYKDERDILKVFVWNPSEVPIMVDDIQIALWKKIQ"
     gene            complement(24783..24855)
                     /locus_tag="PLL59_00095"
     tRNA            complement(24783..24855)
                     /locus_tag="PLL59_00095"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24820..24822),aa:Phe,seq:gaa)
     gene            complement(24921..26267)
                     /locus_tag="PLL59_00100"
     CDS             complement(24921..26267)
                     /locus_tag="PLL59_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22229.1"
                     /translation="MIKKIVSSEALYVSLFWIAYMFLCLHFYVERSTYADNSYYLFEI
                     IQHKDFCIQYNRYSAVLTQWLPVLAVKCQLPLNMVMYAYSLSPVLLFLLLFLFIRYMC
                     QQKYIAYIFLFSLILTVHDSFFYVNDELNQSVGFLLVMTSILKAEKWNNAIRFSLILM
                     LIFILQFAHLFLLLAALVLLVVSYMERRQAISLMSIVFVVLLILARATIFKTGRDGSS
                     MDSLDWHVLTLRNIHSSPFALFTREMIWKYYLPAIVLIVLLCRNIQTKIWSKMALIFG
                     VCGMYLLLVIFLIHGASAIYLDKYLAVLFLLLWSYIAFNLITISKNKNAIFGLILLVN
                     LISFSSLYIEKTYSHRVEYLKVLMAKGTNKQIVLYENMPPDIQTMSWSVPYETLLIST
                     LNGESKTILVKESFYKIEEYLTDSTAFLGAEWTFPQQTKLNEYYFTLKNGLYEYKP"
     gene            complement(26283..27461)
                     /locus_tag="PLL59_00105"
     CDS             complement(26283..27461)
                     /locus_tag="PLL59_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMY22230.1"
                     /translation="MKKYTNFATQAIRTQQERAFREHSVPLYLTSSFTFDDAEQARAL
                     FADEYVDNIYSRFSNPNVNEFVEKMCVLEGTEDGFGTATGMAAVFASMMPFLNAGDHI
                     LASSALFASSTRIIKDYLPKWNIEYSFFDISQPETWESQIRPNTKMLFAETPSNPGLV
                     LIDLAHLCALGKKHNILVNVDNCFATPYLQTPAKYGADIITHSATKFIDGQGRVCGGI
                     VLGNKELIKEVRAFCRQTGPAMSPFNAWILSKSLETLHVRMDRHCENALALAQWLEQH
                     EEIQKTIYPFLPSHPQHELAISQMSQGGGVVTFEVKGGIDRGRKFLDSLEMFSHTANL
                     GDCRTIATHPASTTHSKIDEKVREAAGIFGGTVRVSVGLEHIDDIKADIQQALEKSKI
                     "
     gene            complement(27534..27947)
                     /locus_tag="PLL59_00110"
     CDS             complement(27534..27947)
                     /locus_tag="PLL59_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMY22231.1"
                     /translation="MKVEIERVNEKVHLTAKNEQGVIVHMDGSPEIGGEELGARPMQL
                     VLMALGGCTSMDMLSMLKKMREEVKSYKVSVDAERATEHPMVFTKIHIHYTLEGKLKK
                     ENVEKAIALSMDKYCSVTHMLNSTATITHSYEIKN"
     gene            complement(28075..28785)
                     /locus_tag="PLL59_00115"
     CDS             complement(28075..28785)
                     /locus_tag="PLL59_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMY22232.1"
                     /translation="MKKSIFLFTISALSIATSMARDISEKRHLVGFNAGFSMNTMGLQ
                     KNSTYDNSKNTIKPGGVFGVNYEYRAPKVFGLEIGVNYANRGTQQRLIDDALNRRSFY
                     RLNFHSIELPVTMKFYVGKKKVFNFNVGGFASYAVNVQQRTKVDFKNDALTDIDDKEN
                     NFLGKHNDKDINGNRPFRAYDAGVTAGFEFVSKSGFGVGAKVQQGFIDFTNSKFIVPL
                     DNKKIFHTNAIVYALFKI"
     gene            complement(28923..35063)
                     /locus_tag="PLL59_00120"
     CDS             complement(28923..35063)
                     /locus_tag="PLL59_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HMY22233.1"
                     /translation="MSFLILLLFLTTQQIMAQANYDALWKSVAKFENEGKTKDALSAV
                     EKLIANARNENNTVQTVKALLYKYKYIQVLEENSELKIVVGLKQEINMAKGADKSILQ
                     SILAELYFQYYNRNAWKFSNRTATDIKPSDDFRTWDLKTLFKEINQYYIASLSEKTIL
                     QQIKLDAYTPIIEQVKGSQIFRPTLYDLLANRAISYFNDDKSNIAEPSNAFAIDDKKY
                     FATVNDFITIQLNDADKNAQDYQALKIYQDLLRFRLSDKNNLVALADADLLRIQYVKE
                     HYNNKEENESLYYEALLRLQKEYEKCSIGATINYEIAKYIAANATSENKILNNAAPDR
                     NSIKEALTICDETIKKYPETEGAKNCAALRETFFHKNISITTEEAVVPKTPFKALLNY
                     RNISTVYFKLVPLPYAKKEDILEIKNKETQDDVLKRINAIKPVKSWSITTPVATDFLE
                     HSTEIKVDGIKQGCYVLLASTDKLFTLANGSNAVAMNTIFVSQISYTTRNNNNKEEFE
                     LYVLDRDNGHPLQNAMVKFYRNTYDYDKRKYVRTDIGTATADANGLVTKKVNKSTANK
                     YYYNENFLFEIFYKDDFLPSEQSFYKYFYAREENLNKQKNIQLFTDRSIYRPGQIIYF
                     KGIATQQNKEQHTLLPNEKVSITFKDVNYQDISTQQFTTNEFGSFTGTFTAPTTGLLG
                     NMTLETNYGSTNVQIEEYKRPKFEVLFDDVKKSYNLQDQINISGKAVSYAGANIDNAE
                     VKYTVRRVAQFPSWCWYGWRSPWFPASNEKIITNGITKTDDNGNFIIDFKAIPDLSIS
                     KEFKPYFNYEISADVVDINGETHTASTTVRVGYLAIEANLNVSDNINTSIENKISITT
                     NNLNGQHEGTAVSIKIYELIAPQNPKKSRLWNAPDKFALTKEEHDNLFPYDVYANEDL
                     MNNWQKGMLLNSYAFNTDTQKEIVLPSNSLKSGAYIIEFSCKDKNDNLIGFKKSFIAT
                     SSNDQTPKPATFAYFHIDKNTAKPNETVNIQIGSSMSNTKAILEIAFKGKLIDKKMIT
                     LNNEIKTIPIAIKENYIGGIEVNYVLVNKNRLYSKTTFITVPYEDKSLKIEWLTFRDK
                     LLPGANEQWKLKITGSNKEKVTAELLATMYDASLDAFLPHSFGFYLNQSYFNGYLGGF
                     GNDAFNSKSSLVYTSKKWNPTKEYAYHGYDDLNLFGLSIGSYYSRFLSKSGLAYNAMS
                     MKSESAGAMLGKGSAPKDAENESDSFANEIAPPSPSVQADSAPTQAPTKPAVKNEVTP
                     RTNLNETAFFLPQLQTDADGNILLNFQMPEALTRWNFLGLAHTKDLKSQYFTKSIVTQ
                     KELMLTPNAPRFLREGDKLEFSTKISNLSDRDLSGVADIEIYDAATMQPITTQFIQLH
                     AGANSAITAKDFTVEKGKNIAVSWTLHVPSNIDAITYKIVAKADNFSDGEQASLIVLP
                     NRMMVTEIMPLWVRGESKKSFTFDKLLNNKSKTLKNYKLTLEMSSQPVWYAVQALPYM
                     MEYPYECAEQLFSRYYANSLASYIATENPKIKSIFDKWKNTDALLSNLEKNQELKAVL
                     LEETPWVREAQNETEQKKRIALLFDLSKMADEQQRAFDKLIQMQTPNGGFTWFPGMPD
                     NRYITQHIVAGFGHLAKLNVIDIKNKSKIDEVLQKAIAYLDARLTEDLVNIKKYDVAY
                     LKKNHLYYDNIHALYARSFFNYSIDKKDEEAYNYFINQEKMFWLDQSLYAKGMIALTL
                     HRNKEKASADKIVVSLKQNAINSEELGMYWKANENAGWYWYQAPVETQALLIEAFHEV
                     AKDNNSVDEMKVWLLKQKQTTSWKSTKATAEACYALLLQGKDWIKSDKLVDVKMNKTT
                     INPTQLNAIVEPGTAYYKVNWSGDDIKPEMAKIELKKSDKGPAFGAIYWQYFEDLDKI
                     SGATTALQLSKKLYKEINTNEGKQLISISDKTPLQVGDVIKVRIEIRTDRNLEYVHLK
                     DMRAAGFEPINVLSQYKYQDGLGYYETTKDVATHFFMDWMNKGIYVFEYALRATLAGD
                     FSNGITQIESMYAPEFKSHSKGERVVITTK"
     gene            complement(35118..36053)
                     /locus_tag="PLL59_00125"
     CDS             complement(35118..36053)
                     /locus_tag="PLL59_00125"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HMY22234.1"
                     /translation="MYNLLYATDYAKQENWAALPTMQDNADWTPTGLANKQDSARADV
                     FFIHPTSYFTAIKGNASIDDKMVNKQTDKFSIKYQASVFNESCKVYAPRYRQAALNNF
                     FTSNKERAQLAFDTAYADVKQAFEYYLLHYNNGRPIIIAGHSQGSKHAQRLLREFFDG
                     KALQKQLVEAYIIGFPTHENMFQYLKVSDTPDSYGTYVSFSTFGQNSKIESIVPEYTT
                     AVSVNPLSWTTDNTFVSGQANKGSLSPKNNHIIHDYFGAKNTRGIVEIQKPADKSFVS
                     MVMKNYHHFDYSLFYMNIRENIALRINNFLKHTHQ"
     gene            complement(36210..38891)
                     /locus_tag="PLL59_00130"
     CDS             complement(36210..38891)
                     /locus_tag="PLL59_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMY22235.1"
                     /translation="MFFGNNDKDDKKSDDKNFVKYKFKSLLVYSSDEWMANAAKKYRT
                     VFDRAETTYLRAEFSFYNKLFDEEDWKCKITLKAFDITNNKREELCSLDKEVEVSKLE
                     NIVYVRDGWGNQDAGKFWIKGNYTWEAYIDDVFVEAKLFYVNDVGQVSTEKNPYFSVE
                     YIKLYNGDAEGWRQAANDRKYFSQISKKETKYLWAEIKLTNKTKKDWNYELFLNYYDD
                     AGQHKAVTSRIGKIEANKFDWSYTFDIGWGTDDGGSWKDDRYTIELVFMDTLVAAAAF
                     NCGDVFVEGELELFNGTLVPGIAKAAIENETATVLDDKSLDELLAELDKLIGLQHVKK
                     HIRDQITLINFNKLRKEQGVEDNSSISLHSVAIGNPGTGKTTVMRLLGKIYHKLGLLS
                     KGHVLEVDRVDLVGEYIGQTAPKTQKQIDEARGGILFIDEAYSLARTNEDSRDYGKEV
                     IEILLKEMSDGPGDIAIFVAGYPKEMYAFIESNPGLKSRFSQYFTFDDYLPEELYDIA
                     LSIAEKLKLHIDEEAKKTLKLELTDAFRNRDKNFGNARFAEGVINKAKMLMALRLMNA
                     PDQKSLSKAQLSTIAKEDIVKVFEDRKRTSLKLEINEAELSEALSELHEMIGLQAVKT
                     EIDELVHLIRYYNDIGKDVLNKFSLHTIFTGNPGTGKTTVARILGKIYKALGILERGH
                     TVETDREGLVAGFVGQTAIKTAAKIDEAMGGVLFIDEAYALTDGRGAQHDFGSEAVST
                     ILKRMEDNRGKFLLIVAGYPENMNDFLSMNPGLNSRFDKKMHFEDYTPDEMMQIAETI
                     LKKDDLQLTPDAKLHLNTYFTDLYKHRDKFFGNARTVRQTIGEATKNQNLRMAKIPAA
                     QRTSQDINTIIIEDVTEFVYQPNALRKTVGFRLGGNS"
     gene            39044..40084
                     /gene="thiL"
                     /locus_tag="PLL59_00135"
     CDS             39044..40084
                     /gene="thiL"
                     /locus_tag="PLL59_00135"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HMY22236.1"
                     /translation="MKKEQRTEISSLGEFGLIDRLTKDISLKNTSSIKGIGDDAAIIQ
                     AEKRQVITTDILIEGIHFDLVYTPLKHLGYKSVIVNLSDVYAMNATPKQILVSIAISN
                     RFSVEAIEEIYEGIELACNKYNVDLVGGDTTASPKGLVINVVAIGEQEKEKIVTRNTA
                     KEGDLLCVSGDLGAAYMGLTILQREKQVFLNAPDANIELAGDNYIVGRQLKPEARKDI
                     VDFFAEQQIVPTSMIDVSDGLSSEILHICKQSNVGCQLNEEAVPIASETYNKALEFGI
                     DPINCALSGGEDYELLFTILPSDRSKVKQHPDISIIGEILDENLGVKLFTKGGNYHDI
                     ISLGWNSLKEGD"
     gene            complement(40081..40575)
                     /locus_tag="PLL59_00140"
     CDS             complement(40081..40575)
                     /locus_tag="PLL59_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMY22237.1"
                     /translation="MKKATTINESIWTQFGASLDMLENAIKMCPDEHWNTHLDFWYTT
                     YHCIFWTDYFLTTEPLSFAPPQGYTLSEYEPDKKPDIVYSKAALMLYLTHCRNKAKTL
                     IAGLTTEQLSARWINEYKDYTMLEVLLYNMRHVQHHVAQLNLLLRQSINDAPNWVSLA
                     KDEC"
     gene            complement(40673..41596)
                     /locus_tag="PLL59_00145"
     CDS             complement(40673..41596)
                     /locus_tag="PLL59_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HMY22238.1"
                     /translation="MEVINYSSEPLNKVYGWLPHDLKAERVIFFPDACPGKSPLPTGT
                     VVYTEQEDWRKFAISDCGCGMLLCEADLTKADFKREKWDKIYFDIQANKGKLGDLGSG
                     NHFLDALESYTDDKLYFLIHTGSRNESKLVDDLISQSKKFDTKFQDVCEWAKDNRFAI
                     FKILEKYFGQLHLILDKNHNHFEQTDNGVIIRKGAVKVSPGEQTVVPSNMNGDVVLIS
                     ATKEVEKTYNSLCHGTGRVMSRGEAKEFASTFDYDALREKIYIPEMIANDSIKTDAPF
                     CYRDLDSCLVLIDNLISVDKRFSVFAYLGQM"
     gene            complement(41650..43452)
                     /locus_tag="PLL59_00150"
     CDS             complement(41650..43452)
                     /locus_tag="PLL59_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HMY22239.1"
                     /translation="MKREHKLNSATTFLLFTIFFFCMQCLFTSCKCNSNHAKDIEKKV
                     EAPKDEISFQQDITTILREYIPTLNAIKNDSTKYENWQLVVKEVYLLNNYKSLWLNKD
                     ILSPKGKEMLHVLVTAEFLALNKDLYQYEQLQQIYDSLLSLSPSVDFNLAKQLEVGLT
                     RSFLQMALHLDHGMFADTTQGITSNFWKDKNKYIQLLQTAVTDSIFKSLSSLEPNNPL
                     YNRYMSALRAFVAKNNISATPISIRNPKLDSLGALTDARKALVYHHYLMDSLKNNDSA
                     FIVALKRFQKENNLNADGTIGANTIKALERDNSKKFQLLAINADRWRKEHIIELPERY
                     VWVNLPSYRLKIIDHDTLVLEKKVVIGKSNLKNETPILESAINQIVLWPTWSVPQSII
                     KHEMKSFKGYVVTKNNGWTSVVQPPGPRNALGVVKILFPNKYSVYIHDTPTKSTFGAD
                     LRAASHGCVRCQDPLEVVANLMMMDTFKITYDSLIALKDSKIATRTFRLKKPVPVYFR
                     YFTTEADFNGEVKFYSDVYNRDKQLINFIFNGKQAHKLTKEEIRERQIQDSVALIKKK
                     IADSIAATKKALADAEKFIKKDSTVIIHQDSLVN"
     gene            complement(43512..46082)
                     /locus_tag="PLL59_00155"
     CDS             complement(43512..46082)
                     /locus_tag="PLL59_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMY22240.1"
                     /translation="MTRFLHIILLILVPLCAFSQFEEHFTDGGFNVNPHWDTLGTNFI
                     VVNQVLNLQASNNTGNAAIMTADTLGKSVEWSLYLRLGFQPSDNDKLDVVLMSNQMDI
                     RNDFDGYFVRIGQNGNTDALDFYRKDGNSNVLIKSMLAGNFQNGADGNLKVVKNNLGK
                     WVFYWKEANQSSYVALDSTQELTYASTTHFGMLCTYTAASKDSFWFDDVYCIQRPLLS
                     LDVTPPSVSTVSLINSTQVDIYFDETVDITSAQLINNYTLSSLGNPMTAVIDALNKKL
                     VHLTFAQRFLSNTTYTLTTNNVADSAGNSMSIPNISNFNTPYYAVLNDILINEIMIKP
                     STALGLPNKQYIELKNTSNENISLKNFTINNANVLEGTIAANTYAILCAAADTNLFKP
                     FGNTIALDNWNNLAFSGTLTLRADDTILIDQLNYVDTMYHDVIKQNGGWSLELLSSAY
                     TGDCDKELFWSASVDVNGGTPGEMNRNEAPEYVHATFTLINNTTIEVDFTKPMDKTSA
                     QSISNYQIDNGISIQNIMLANTYASKVTLTLASALQPNIIYTFTVAGFSGCVKYIHLA
                     DTFDIAITEIPTNGELIINEILFHADAGVEQFVELYNNSSKLFKISDIKFAQANPNTE
                     IEEQLLDLSNTEGYIFANGYIVFTRNKISIKSQYPPAILEKMKDVNLPTFDTQEDIVL
                     LKNRNDEVLDKLHYQTSWHFPLLKTTKGVSLEKSGFDLPQNKRYWHSAAESAGFATPT
                     YLNSENEYHLLGNVHIIPEVFSPDGDGYDDEAKITYSFDEDGSVVNVYLYNAAGQLAN
                     HLVKDEAIAKEGAFVWNGDDENGSKKDVGIYFLVFERKKPDGEKIIYKRKCVLAAKLN
                     "
     gene            46262..47257
                     /locus_tag="PLL59_00160"
     CDS             46262..47257
                     /locus_tag="PLL59_00160"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HMY22241.1"
                     /translation="MKVAVVGSTGLVGGEMLKVLTERNFPVTELIPVASERSAGKKLA
                     FKGKEYTVVTMQTAIDMKPDLALFSAGGSTSLEWAPKFAAAGITVVDNSSAWRMDPTK
                     KLVVPEINADALTKEDKIIANPNCSTIQMVVALNPLHKKYKIKRVVVSTYQSVTGTGK
                     KAVDQLMNERANGSSDDMAYKYKIDLNVIPQIDVFLDNGYTKEEMKMVKETNKIMRDE
                     DIKVTATTIRIPVMGGHSESVNIEFENEFELADIFDELSKAEGVVVVDDPSNQKYPMP
                     MDAHGKDEVFVGRIRRDETQAKTLNMWIVADNLRKGAATNAVQIGEYLLNNKLIG"
     gene            47357..48925
                     /locus_tag="PLL59_00165"
     CDS             47357..48925
                     /locus_tag="PLL59_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HMY22242.1"
                     /translation="MPIRMVDDDNQQQDYVPRNDGGGGQRGGGGGLPGGGCLTAFLPI
                     ILKLIFKKPLIGIPILLIGGFILFKFGGSGLPTNTNNSNATALATGAELKEEVYDKAE
                     VAASLSEYEKNPLPEYVSLLKFAPARMDQGEQGSCVGWGSSYAARTILEASSTGQNPD
                     EIAMSPSFVYNQIGLANCQGAYILNACKVMSEAGDIPKKYFEYTDQSCTREPNQQILQ
                     AAQQFKIRGYERLSVDGDDYTTDFFAIKQYLAKGAPVICGMMVGGSFMQEMQGQKLWN
                     PTQYDYDMEGFGGHCMCIIGYDDRLDGGAFQIMNSWGPDWGENGIGWIRYKDFMHFNK
                     EAYALQPLPKRGEMAARKFECTIGLVNNDTKQYINLTNSGNIFSTTTPIAKGTKFKIE
                     VQNSIECYIYIFGQETDGSSYVLFPYTPKHTAFCGITGYRLFPKNQSLQADNIGEKDK
                     MAIVVTKEPIDYNQINTAINKAQGDYKTKVENALNAMALSHVEYSANKGRIAFTAESE
                     NKNAVVSVIEIDKK"
     gene            48932..49399
                     /locus_tag="PLL59_00170"
     CDS             48932..49399
                     /locus_tag="PLL59_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HMY22243.1"
                     /translation="MSFLIYGAAGYTGKITVESAVKQGLKPTIAGRTESKIKPIADAF
                     GLPYLIFGLDDIDNITSHLKNFSLVLNCAGPFSSTIKPMLKTCLQSGTHYLDITGEIE
                     VFEYAKAFGKKIIERNIIVMCGVGFDVVPTDCMAKHLHEKMPDATHLELAWTG"
     gene            49387..49977
                     /locus_tag="PLL59_00175"
     CDS             49387..49977
                     /locus_tag="PLL59_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22244.1"
                     /translation="MDWLGANISHGTMTTMVESLGKSGAIRKDGVIALTPIGHIGKEI
                     DFGTKKIFCMTIPWGDVSTAHFTTKIPNIMVFTAVPKNAYYAMKMQSLFNPIMRTSFV
                     RNKLQAYVDKNISGPDLDRREKGISLIYGKISNAKGETIEARLQTPEAYKLTAEMALI
                     ITQKILSDKSLPAGYHTPAELFGANLILEMPGSKYV"
     gene            complement(50027..50950)
                     /locus_tag="PLL59_00180"
     CDS             complement(50027..50950)
                     /locus_tag="PLL59_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMY22245.1"
                     /translation="MSLLVVGTVAFDAIETPFGKTDKITGGSGNYICQSAGHFTNDMQ
                     LVSVIGDDFDQNELVYLKSIGTDIEGVQVKEGEKSFFWSGKYHMDLNRRDTLATDLNV
                     LATFDPILPAKYKNAEFLILGNIDPALQAKVISQMEVRPKLIAMDTMNFWMEIALEPL
                     KEVLKMVDLLIVNEEEARQLSNEYSLVKAANKIREMGPKFLIIKKGEHGALLFHGNRV
                     FFAPALPLEDVFDPTGAGDTFAGGFMGFLAKTKDLSFDNMKRAVIYGSAMASFCVEKF
                     GPERLKEVSEEEIEERVQVFIELVDFDIILQ"
     gene            51048..51124
                     /locus_tag="PLL59_00185"
     tRNA            51048..51124
                     /locus_tag="PLL59_00185"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51082..51084,aa:Asn,seq:gtt)
     gene            complement(51266..51892)
                     /locus_tag="PLL59_00190"
     CDS             complement(51266..51892)
                     /locus_tag="PLL59_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HMY22246.1"
                     /translation="MKKRIDIDNWNRKEHFLFFSKFEEPFFGVTIKVDCTSAYKKAKE
                     KGVSFFLYYLYRALKAANDVENFRYRIIENQVFEFEKVNASPTINRPNGTFGFAYMDY
                     FEDEKYFYEKALEEIENVKNTSSLLPAVSSENVIHFSAIPWLDFTSISHARSFSHPDS
                     CTKISFGKMTEINGKREMAISIHGHHALMDGVHVGLFVDKFQVLLNNE"
     gene            complement(51894..52586)
                     /locus_tag="PLL59_00195"
     CDS             complement(51894..52586)
                     /locus_tag="PLL59_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HMY22247.1"
                     /translation="MNRDITVIAFDADDTLWVNEPYFQEAEKQFCILLENYLPQHTIS
                     QELFKTEMKNLALYGYGIKGFVLCMIETANRVSNQTVSLKVIDKIIEIGHDLLQKPIE
                     LLQGVSETLEALNGDYRLVVATKGDLLDQERKLKNSGLEHYFHHIEIMSEKKESDYQK
                     LFKHLDCQPENFLMLGNSIKSDVIPVLELGGKAAHIPYHVTWTHEQQETNLTHENFIQ
                     LKSINEILNYLK"
     gene            52667..53233
                     /locus_tag="PLL59_00200"
     CDS             52667..53233
                     /locus_tag="PLL59_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY22248.1"
                     /translation="MLKTNESFLSYLSALYERQSRKEDIILKRCGKGQRILSQNEIAT
                     KVMLIVEGISKCYFTEDNDKEYIIEFLGKGEIIGELELIRNIPCLCTIEAMTAVSCFA
                     IDKPYFSSLLQTDIHLNHLLLDSFAKRIVDTASRAAYQQLYTVEHSLNKLMELQSIQN
                     VSLSKDEMASYLGITVRSLNRAMKNLRE"
     gene            complement(53411..55429)
                     /locus_tag="PLL59_00205"
     CDS             complement(53411..55429)
                     /locus_tag="PLL59_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22249.1"
                     /translation="MSKKANKPKSIPSKPTPKPAVKANTKQYTYLAILVLILITGIVF
                     LPSLSADFVNWDDAFNVYENASLKPFATTWSWSAVKQIFSTHVIGNYNPLPIFTFAVE
                     QYFFAPNPLENPFPFHLNNVCLHLACTLFVFLIFNRLGISKTAAFIGALLFGIHPMRV
                     ESVSWITERKDVLYGLFFLATLYSYLTYIQTKNSKKKWYFIALLCSVFSYFSKVQAVT
                     IPLSMVAIDIFLKRDWKSIKIAVFEKLPWWLLSLIFGLINIYFLQQANSLQSTDTTVT
                     YSFVDKLAVGAYAYAIYTIKWIFPYKMSPLYPYEPTLPIIAYVCLIIVPILLLGSIFI
                     TWRKKLHALLFGILFFTFNVMFLLQIVGAGQGFLADRFTYIAYIGLFFITSFYYDKLV
                     GKYPTKIRLIQLCIVAYLCLFAYLTIQQQKVWQNGGTLWEKVKSYYPNSPTAWKNAAE
                     YYRDTEKNRQKAIENYQQALMLQATDATTYNNLAKAYTDSSFSATLNAVQRESILMKA
                     LTAYNQAIINYPQTQNKDNKLIGEMYVNRGAVNAMLGKLDTALLDFNKGLSLYPNNLN
                     GYSNRAILHAQMNRFDFALKDRDAYIMLQPNNADMYYERGVCNVQLGKNKEALLDFNK
                     AIALNNANGIYYVGRAHVQRLLGNTSAQQQDVATAKQLGAQIPEGLMQ"
     gene            complement(55458..55640)
                     /locus_tag="PLL59_00210"
     CDS             complement(55458..55640)
                     /locus_tag="PLL59_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22250.1"
                     /translation="MKKSRKEKKLITIIPKKNSTVATNEHKEIAAVDMQLPFVLMPEL
                     DELVKKNPNRLIGCGG"
     gene            55673..56695
                     /locus_tag="PLL59_00215"
     CDS             55673..56695
                     /locus_tag="PLL59_00215"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HMY22251.1"
                     /translation="MHRFLQRYTGFLRNIRILHYLYNFLHQKGLKHNQHLYPFFGINK
                     SIYSSISHKDFIGKKAKAPWLDAIVSEKDIQNHPEFGCFPTNIEAQLMQWKKNGFIIW
                     ENFLDTETVDIINGEIDALLEKNSVGFNYTNTKIFNAYKHAFSIRKIMKDKRLLQLFS
                     FILNRKVIPFQSINFLKGSEQRAHSDAIHMTTFPQGNLLAAWFALEDINIDQGPLSYY
                     IGSHTLPYITNADYGNSANKWMLDGEANEKYEKKIAQIIQEQHLQKKVISIKKGDVFI
                     WHANLLHGGEPQLNKDLTRKSMVVHYFASDVIAYHEISERPAIFDVELVGEIEDDFYK
                     GQTHLL"
     gene            complement(56696..57418)
                     /locus_tag="PLL59_00220"
     CDS             complement(56696..57418)
                     /locus_tag="PLL59_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22252.1"
                     /translation="MSGILVKYNCRILPFGIWASVAIACGIALNVMYVLIYFDAPEML
                     STVALVGLIALIIYLLMSEATFTITDKTIERNLSSKSALFRKNSNKIYTWNDIKSYKS
                     GTDKGRYRGEYQFVTIYFKNGDTWEFNDNYGEQSIAFGAFLTVFLEKTKLHNEQAQRS
                     KQEATASIHTEVPVLEIERKKTFYETIFAKILTIVFIVFIVAVYVYGKTYMSATSYFK
                     LYIILIPGVAYMYYRTFLSSKK"
     gene            complement(57422..57751)
                     /locus_tag="PLL59_00225"
     CDS             complement(57422..57751)
                     /locus_tag="PLL59_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22253.1"
                     /translation="MEDELKIQQYTLQLLEENDISFHSFSEFRNFIIEKINNWINNDF
                     EHLLYLLYRIDVHEDKVRKLMIEHKGENAAEIITDLIIARQKQKIETRKLFQFNPAAD
                     ISEEELW"
     gene            complement(57825..58583)
                     /locus_tag="PLL59_00230"
     CDS             complement(57825..58583)
                     /locus_tag="PLL59_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HMY22254.1"
                     /translation="MISQHTIDLLNFYHDKYNNKNFIDADPISIPHRFSSLQDIEIAG
                     LFAAVLAWGNRKAIINSCNKIMKLMDNHPHDFILHHSPKDLKPFTSFVHRTFNATDVL
                     YFIHFLHHYYSKNKSLETLFVANTIEDGLQQFKQAFFALEMAPQRTQKHISSPANKST
                     CKRLNMYLRWMVRCDKQGVDFGLWKQIKPAMLMCPLDVHVENYARQLNLIQRKQRDWL
                     TVTELTRNLSMLDKNDPVRFDFALFGMGIEKIIV"
     gene            complement(58580..59266)
                     /locus_tag="PLL59_00235"
     CDS             complement(58580..59266)
                     /locus_tag="PLL59_00235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HMY22255.1"
                     /translation="MSYTLVILAIAPALAICGYIIHKDRFDKEPIWLMLLAFFFGVFS
                     IFPAGVTTLIGESILGTNASIINTLIHAFLLVALSEEFAKFFFLRYILFRRKEFDEPL
                     DGIVYSVMIGMGFAAFENIFYVAEGGIGIALMRMLTAIPAHAIFAIVMGYYIGQAKFD
                     ITHKDDLIRKALVYPTLLHGAYDFFLFQKNIPALSLLSIIGVWIAGKKVFYILKNASE
                     QAAENKDKRI"
     gene            complement(59310..60449)
                     /locus_tag="PLL59_00240"
     CDS             complement(59310..60449)
                     /locus_tag="PLL59_00240"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HMY22256.1"
                     /translation="MKFATKVIHAGIEPDATSGAIMTPIFQTSTYVQDAPGVHKGFEY
                     ARTHNPTRHTLQNQLAALENGKYGICFASGLAATDAIAKLFNSGDHIISCNDLYGGTY
                     RIFTKVFGKFGMDFSFVDMTNIVNIENAITAETKLIWIETPTNPMLSIVDIEAVCALA
                     KKHNLLVCVDNTFASPYLQTPLDLGADIVVHSATKYLGGHSDVIHGAVIVKDKTLAEQ
                     LYFLQNACGAVPGPQDCFLILRGIKTLHIRVQRACENAAKIAAFLQSHPKIASVNYPG
                     IPTHKGHDIAKRQMRDFGAMISFSLKDDSIEAANKILSSTALFSLAESLGGVESLIGH
                     PATMTHASIPLTERLKTGVVDSLIRLSVGIEDAEDLIEDLKNAIG"
     gene            complement(60951..61082)
                     /locus_tag="PLL59_00245"
     CDS             complement(60951..61082)
                     /locus_tag="PLL59_00245"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMY22257.1"
                     /translation="MDWLASDMSIEEIIEDFPQLNKQQILACVAFAAEKERKLKVSA"
     gene            complement(61082..62335)
                     /locus_tag="PLL59_00250"
     CDS             complement(61082..62335)
                     /locus_tag="PLL59_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HMY22258.1"
                     /translation="MINIRPFKGLRPRKDIVDKVAAKPYDVLNSAEAKAEATNNPFSF
                     LRISKPEINFENNIDQYAEEVYQKGKEVFTTFINENILQRDEKPCMYVYAQTMEGRTQ
                     TGLVAGSSIDDYFNDKIKKHELTLIAKENDRIKHMKTKMAQPGMVFLTYKAVEEIDVI
                     IEHYTSHQAAENDFVDSQNVRHQLWKIDNENIIQKLVTLFKEQVPASYIADGHHRSAA
                     SAKVGKQLREENPNHTGNESYNYFLSCLFPSNQLQIQDYNRLVKDLNDLSEATLLEKI
                     AQHFTIEKIGQTIYKPTQLHEFSMYVNGNWYKLNANKNTYNENDPIGVLDVTILSKYI
                     LDEILNIKDQRTDKRIDFVGGIRGLGELQKRVDSGEMKIAFAFYPVSLDQLIAIADTG
                     NIMPPKSTWFEPKLKSGLVINVLND"
     gene            complement(62332..63993)
                     /locus_tag="PLL59_00255"
     CDS             complement(62332..63993)
                     /locus_tag="PLL59_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMY22259.1"
                     /translation="MRKLILTVNCLLFTAYFSQAQMYQQKMATFLNLIDNYYVQSANI
                     DSLVEIGITEMLKHLDPHSVYMNATEIKKANEPLEGNFEGVGIQFQIYQDTIQVIHVI
                     AGGPSQKVGVQDGDKIVYVDTTKVAGVGINNDGVFKNLRGKKGTKVTIKVKRSGEPEL
                     LEFEIIRDKIPIFAIGAQYMATPEIGYVKLERFSATATEETKSAIDSLKKLGAKKIIL
                     DLTGNGGGYLNQAHTLSDLFLGDDKLVVYSEGRSQPRIDLIATAKGNFEKGDLVIMVD
                     QSTASASEIVSGAVQDWDRGLVIGRRTYGKGLVQKGYSLPDMSAVRLTMAHYYTPSGR
                     NIQKPYEKGNEVYQEDLEERYKSGELFDSSKVKIIDSTKYYTNQKRIVYGGGGITPDI
                     FVGLDTSWSSKFYGKLVRSGSFNQFVLSYVDKNRTTLEKKYPTLDIFIKQFHVDSTIL
                     HSFYEYAKSKKIEPDSAENYSKSISTIQIQLKGLLAQNLWNSSAYYQVTNIQNPIYIR
                     ALHAFTDGSFQKYGIATATPIKIKKTTKKYKKKKYSNNKKPIEKK"
     gene            complement(64419..65201)
                     /locus_tag="PLL59_00260"
     CDS             complement(64419..65201)
                     /locus_tag="PLL59_00260"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HMY22260.1"
                     /translation="MADTKRKIEICIISDIHLGTRACHAAELNRYLQSIDPSVLIING
                     DWLDIWNFGAGYFPKEHIENVFLILDMARQGIPVYYITGNHDDHMRNFSGYKFDTLQL
                     RDELILEIDGKKHWIFHGDKYDLSVGGSARWLAKLGGRTYDKIWQINRLVNNVRMKYG
                     KDRIFISKYIKDKVKSIVKSKVQDFEDVAIEIGIEEGYDYIICGHIHKPQNRIVSNEN
                     GSATYLNSGDWVENLTALEYVNGKWDLFYYQHDLQYKGIDRS"
     gene            65402..66424
                     /locus_tag="PLL59_00265"
     CDS             65402..66424
                     /locus_tag="PLL59_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HMY22261.1"
                     /translation="MSYAKLEYIWLDGYKPTQSLRSKTRIERDFSGKLEDCPTWCFDG
                     SSTEQAPGGSSDCLLKPVFICADPARKFGYLVMCEVLSPDGTPHETNGRATIEDNDND
                     FWFGFEQEYFIWDLKKDKPIGWSDFTVTGEPGPQGPYYCSVGGGKAVGRYMVEEHLDL
                     CLEAGLNVEGINAEVAIGQWEFQVFAKGAKAAGDQTWIARYLMERVAEKYGYAINWHC
                     KPLGDTDWNGSGMHANFSNEVLRTCGSKETYETICNAFAPFVKEHIDVYGADNHMRLT
                     GKHETQSIDKFSFGVSDRGASIRIPIATVDRGWKGWLEDRRPNSAADPYKVAARIVKT
                     VKTANV"
     gene            complement(66554..67240)
                     /locus_tag="PLL59_00270"
     CDS             complement(66554..67240)
                     /locus_tag="PLL59_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HMY22262.1"
                     /translation="MQKIQLSLQKRTIDAYFFEASSNQIRPAIIFLPDLSGIQKTTIK
                     SAEILCSDGDYHVIIPDLYSGGSSLRKYCMQFLMSSIARNNEANNNAPLDEFFEIIEA
                     VKSLDLIDENNLGVVGQCLTGGFVLHAALRPEVKAPVVFHHSLGMKGSGMPKSCSSLI
                     DKKVQGHFAYVDAFCPVWKVRQLETELNGNLETHWYNLPHGIPHLFFNTAQGQKAFES
                     MSAFFQEQLQ"
     gene            complement(67272..67991)
                     /locus_tag="PLL59_00275"
     CDS             complement(67272..67991)
                     /locus_tag="PLL59_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMY22263.1"
                     /translation="MYDFHFKEIDADGQTTLEAIDKADNFNEWMYQTIKPFCKGKVLE
                     IGSGIGNISRYFLRDKFDITFSDIRTSYCDVLLQKFPSSKVLEIDLVDTNFDIKFAHL
                     MNTFDTVYALNVVEHIKDDSLAISNAKKLLKEGGNIIILVPAYQALYNRFDVVLEHYR
                     RYTNTTLNALIGKHFKIEHSQYFNFIGIFGWFFSGSILKKKTVPAGQMSLFNKLVLVF
                     KLVDKILLNKSGLSVIAVGKK"
     gene            68073..69443
                     /locus_tag="PLL59_00280"
     CDS             68073..69443
                     /locus_tag="PLL59_00280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMY22264.1"
                     /translation="MFDFLKERKLSVLLLLALALRLLFFWVYRPWDPSVERNLVLNGD
                     AVQYHYLGIAILKTFGFADNVFRTPGYPAFIAVVYSLFGIKPYIVLLIQIVISVASIY
                     YVYKIGLLCFNKTVGWFAAVIMALDPHQITFASWLFADTFFAFLFIISIYTFVKGITE
                     KRIKYIVISAVVLGFNILTKPVIQLFPVCFLLLCLVYQKADLKTRLKYGFIYLLIPFF
                     IALPWLLRNKIKYQHFAISSIVGFDKLVYSVPLTEHSLTQKSNGQIIDSFLTKIKIEN
                     PQLKNIPENCTKIWLNITFEATDVYGKYADEYLAKHRIAYLKLHVKGMLKLMLNMGTQ
                     NMLDKLHIENKKWDDETRYTSGFFQLAKKFVLTKTKTEIILGLFIVGFLIVCYVYGFI
                     GVYYCLKNKHVFWLFLFGGAILYFLLIYGKLPIVRFKLPITAMYSVLSAYGFYCFHVL
                     KKEKLS"
     gene            complement(69438..70025)
                     /locus_tag="PLL59_00285"
     CDS             complement(69438..70025)
                     /locus_tag="PLL59_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HMY22265.1"
                     /translation="MNTLYPIFIKTENINILIVGGGSVATEKIRFILKNSPNANITLV
                     AKEISAEIRKQSRNHKTLVLMEKAFEENDLNNMQLCIAATANKELNHQIYLDAKQRKV
                     LVNVADTPELCDFYLGSIVTKGDLKIAISTNGKSPTLSKRLREFFETILPENINDLLE
                     NLHEYRSSIKGSFEEKVNALNEITKSFVSKNNNEL"
     gene            complement(70049..70789)
                     /gene="cobA"
                     /locus_tag="PLL59_00290"
     CDS             complement(70049..70789)
                     /gene="cobA"
                     /locus_tag="PLL59_00290"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HMY22266.1"
                     /translation="MTKKLILVGAGPGDPDLISLKGIKALQQANVVMYDALVSEELLQ
                     YAPNAIPIFCGKRAGAHYMKQDEINSCIVENIKKHGTVVRLKGGDPFVFGRGQEEAEF
                     VASHGFVTEVIPGISSATALATAQNIPLTKRGITESIWVTTGTTSCGELSADIELAAQ
                     STATVVILMGVGKLAEIIQLFTKYKTENYPIAIIQNGTLANEKVVCGQLSNIYLKVQE
                     QNITAPAIIILGKVVDCAEIYACKINKT"
     gene            complement(70818..72941)
                     /locus_tag="PLL59_00295"
     CDS             complement(70818..72941)
                     /locus_tag="PLL59_00295"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HMY22267.1"
                     /translation="MSITITKNITPEAAKDIFELQQKVNAYKNGEIPEDKFKAFRLTR
                     GVYGQRQLGVQMFRTKFPFGKITPAQLLRLADLSEQYASVNLHLTTRQNIQLHHVKVT
                     DAPAMWAGLEDVGATAREACGNTVRNITGSSIAGIDPDELFDVSPYARETFEFFLRYP
                     INQDMGRKFKIAFSSSDKDSAYTFLHDVGFIPRIENGERGFKVVVGGGLGAQSILAQT
                     AYEFLHEDQIIPFVESVLRVFDRYGERVRRHKARMKYLIKDLGLEKFMELVAAERTAN
                     KVKSYKIDTTKVSIGEPLHHNIAPQVTIEDEKAYQDWLRTNVFEQKQAGYFAVALKVT
                     KGDVKPDEARAIAALVQQYASEDMRITMNQGLLLKFVPKASLPYFYQELHKLGFAKVG
                     FDTIADVTTCPGTDTCNLGVTNSMTLGTVLEDVIYDEFYHLIFDSEIKIKISGCMNSC
                     GQHMAANIGLHGSSIKVGERVAPAMQVILGGGVDVHGVGTVGDKIVKIPTKRSPEMLR
                     IVLNDYEINSNEGEYFNDYYRRQGKNYFYALLKPLANVSLYVDDDFIDYGGVENYIQE
                     IGVGECAGVMYDMVGGIIKDADNKLIEAKDHLLKNKFPEAIYYAYTGFIIGAKALLLS
                     KEIECNTQIKMLKDFDEHLVATGEFKMENGFENVVLQINQHEPEIAFATNYVQQFECF
                     LKDVIAHRTAAANKDKVVVESSYKA"
     gene            complement(73173..73766)
                     /locus_tag="PLL59_00300"
     CDS             complement(73173..73766)
                     /locus_tag="PLL59_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HMY22268.1"
                     /translation="MDNTTIKPVMIYTESTPNPNTLKFVTNKALLLNDAVEFQNIEET
                     TEAPLAKALFGFDGVSNVFITNNFVTITKTDEHIWHEMMIPIKDFLKKYIDEGQTILS
                     DNFVKPTKTATNTINDDDSDVEIRIKGALDKYVKPAVEMDGGNISFVSFEDGKLTLQL
                     QGSCSGCPSSTVTLKQGIENLLKRFVPEVQEVVAENE"
     gene            complement(73818..74579)
                     /locus_tag="PLL59_00305"
     CDS             complement(73818..74579)
                     /locus_tag="PLL59_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMY22269.1"
                     /translation="MSNTILITGATSGFGEASAQIFAKNNWNLIICGRRNERLKALEK
                     ELISNYSVRVISLCFDVRNLQETKEAIASLPNDWKNIDVLLNNAGLAAGRGPIQDGVY
                     DDWEQMIDTNVKGLLYMIREVSPLMMARKKGHIINVASLAGWEAYGGGNVYCGTKHAV
                     RAITRSARVDLLPYHIKVSVISPGAAETEFSLVRFKGDEQKASAVYEGYQPLKAEDVA
                     NSIYFMATQPAHVNIEEIFILPAAQATATITQKNA"
     gene            complement(74642..76294)
                     /locus_tag="PLL59_00310"
     CDS             complement(74642..76294)
                     /locus_tag="PLL59_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22270.1"
                     /translation="MLTAVKAFFFHKTEEQFSYAKLCLGMLLLLLCGIRLLRNFNQLV
                     DISFDDEVKYMRYGLDMFAQIKNDWGPTYNMWYKLLSLFEHRPIELYLLNYKIIILLT
                     ASTLFAFLYSYGISFIASMWMALCMLVSNVNIVNYPRISAFVVALFLWCLIINKLFIT
                     SKSKQYILICFVLFVAAFARPELMLSFFLFLIFTMYYIYQSKTFKATLVFSIPFIIII
                     MLLLVFVQLPANTYMGKDRIYGVFCQHYTLKYIYQNKLDYALFIDWIAFSKKQFPNCN
                     TFLDIVKNHPLTVITGILTNAIIFLLVVLASLSEFLYPKFLFYRKYLEILSVVLLIIL
                     PLFALIPKQKRQIFWSRVTAEREWLLILLVFALPGLASASVFFPRPHYLLFLLIPLLY
                     TLSIVFEILFDIKHIRTVSIFVLVGIFFWRMPDMQSYKTPRIISGPCPNQSYKVLIKM
                     LNERNQHQHVIFSNILNLSMMADKNYTDFSAEQDYNNEQTFIEQLKARKVDNVLVTDF
                     LLQDLRLKNDSTWQHFIQYPNKYGFVKKMPFKDCSTYLLYKE"
     gene            76361..76687
                     /locus_tag="PLL59_00315"
     CDS             76361..76687
                     /locus_tag="PLL59_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HMY22271.1"
                     /translation="MTLQEAQQTIDTWINTTGVRYFNELTNMAILTEEVGEVARIIAR
                     QYGEQSFKASDKNKELADELADVLFVLICIANQTGVNLTDALAKNLAKKTIRDSERHK
                     NNEKLK"
     gene            76926..77378
                     /locus_tag="PLL59_00320"
     CDS             76926..77378
                     /locus_tag="PLL59_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HMY22272.1"
                     /translation="MRTLVKTSCGTPSNNRFFFDDVFGKDFFDFAPAFNGKNVPAVNV
                     KETETEFSVEVAAPGFQKEDFSITLYENILSIAVEQKSEKNENDDKAKFSRKEFSYAA
                     FKRSFTINEDSIDTEKIAAKYENGILLLQIPKKGKTEIEKKEKTISVA"
     gene            complement(77499..78620)
                     /locus_tag="PLL59_00325"
     CDS             complement(77499..78620)
                     /locus_tag="PLL59_00325"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY22273.1"
                     /translation="MNKMMYLLMLLFLTQQITFAQETEKSALEKAKEAEDYGDIEMAL
                     KWYKKASELNPKNGTTYYDITWCQNELGLYDDAVKTATNGIKTSTNAKLLYEFGYAYY
                     KLGNYKEAIIKYKNALSVDANEHSALKGIADVYFDNKEFSKAKDYYEQCLDKSKDVSF
                     SNYRLGYIMNEEANYQKAVDYELASLKLDSEYAPAYNELAYAYSQLNKKSMALDNYIK
                     ASNLNPKNAIYLSNVGDLYYGVKELEDLDKALEYYKKSFAIENNNAITNYRIAWILNE
                     KEAYSEAKPYLYKAVEIDPKFADAWIELGWIDYSEKNYKAAESDYYKALQYDSKSDLV
                     RYYLGQVFVKQKKTKQAKKMLEELKSMNSQYAEKLEKML"
     gene            78834..80483
                     /locus_tag="PLL59_00330"
     CDS             78834..80483
                     /locus_tag="PLL59_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMY22274.1"
                     /translation="MIIQTPLEKLYHWTAQKPDQTFLRQPYNGEWIHFSWKKTDDEVR
                     RMAAYLRSLNLPPQSKIAIISKNCAHWIMSDLAIMMAGHISVPLYPTIPADLIRYCLE
                     HCEAKVAFIGKLDDWKNQRAGLPDGVKGISYPFWTEEGYENWDDIIAKTPPFTEMYVA
                     KSEDIATIIYTSGTTGLPKGVVQSVHTFSYAVCWALTTLDDLTDKERFFSYLPLSHIA
                     ERMLVVMGGLYSGGSISFAESLDLFAANLKDTKPTIFLAVPRIWTKFQMGILSKMSQQ
                     KLDVLLKIPIINNIIRKKIKEGLGLEHAKYLLTGAAPLPVNTMLWFEKLGFVIYEAYA
                     MTENCAYSHLNRKGKRKPGYVGVAMPNCDVKISEEGEVLVKSEATMIGYYKEPEKTAE
                     TITADGYLCTGDQGEIDAEGFLKITGRVKDIFKTDKGKYVAPAPIELELSKNAMIEQV
                     CVVGANLPQTMALVVLSADARNESDDTIAKSLEETMHDVNKLFDKHEKMKKIIVMREE
                     WTVDNNLLTPTLKVKRNILEKAKNTHYEKWYKDANTIIWEN"
     gene            complement(80542..81348)
                     /locus_tag="PLL59_00335"
     CDS             complement(80542..81348)
                     /locus_tag="PLL59_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22275.1"
                     /translation="MKKFLLPFVSFLFVLFATTSCKKIINNATTNPVPPTPTITAPGA
                     YYGTLTSIRTYFEFDPNKFSPVKLPVALPMMNIDLETAVASFSTTTSSTTLTDAGAVD
                     VNTFSLTKADNNAYYRNGFSPTSPTNVDLNFSSGSNWNVAGSSSVTAFTYNHNVAFPS
                     YGGLATLPTTVTKSSNLSLNISSSNADSIYVAILSGSSTSTGTVIKRYGGTATNIVIN
                     SSDLSALTTTGSDGIAYLEVIPWRYNTATLNSKDYVFMKLAAYVKNIEIE"
     gene            complement(81535..82077)
                     /locus_tag="PLL59_00340"
     CDS             complement(81535..82077)
                     /locus_tag="PLL59_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22276.1"
                     /translation="MALFSMTAFANGNDVSIEPSNITVENNSEATAEEYVEYDRRFSS
                     ITEVGTAFAGKQAYFNLYQAIGMRINPYFFIGQGVGIQVSNNRIYQMHTTADFRAYVL
                     DKKVTPMFVVQAGINKVGNAPETETKQLKDLQLMLNAGTGILIKAKPNASFTINGGYT
                     LYSDFKQNIHGGFVKVGYVF"
     gene            82403..82654
                     /locus_tag="PLL59_00345"
     CDS             82403..82654
                     /locus_tag="PLL59_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22277.1"
                     /translation="MEKQNIQHLYFYLAVLCALWFASTSWIWTYLFALFISYPIGLLA
                     FIFWYRGSNIDENKNRYNLPKYILIFGLLSSIISFLLML"
     gene            complement(82677..83102)
                     /locus_tag="PLL59_00350"
     CDS             complement(82677..83102)
                     /locus_tag="PLL59_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMY22278.1"
                     /translation="MENKHTSITIHTTIHASIEKVWNCWTMPAHITEWCSASEDWHTP
                     SASNDLQAGGSFTTRMEAKDGSFGFDFGGIYDEVILHQKISYTMGDGRTVNILFSGDE
                     IQTHITETFDPEDHHPLDMQQAGWQAILDNFKRYTEGIA"
     gene            complement(83142..83855)
                     /locus_tag="PLL59_00355"
     CDS             complement(83142..83855)
                     /locus_tag="PLL59_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22279.1"
                     /translation="MTTIVFEVLAEGGSLSIERIISNTGTKFIYHHQEIDFTDEGLAI
                     NKNGEYETFEQAFQIINHRFPWYKLHLHIVHEDYRAFVLKHLLFALNKNNITPMDWQN
                     AKTDAEKALQIQLEYAYTTLENGLQKIKVKNLIELTEYDYQEFSDQYAQKIGQKYKLN
                     GTYKMWTDEQTFDPIKMEVLNVSNSFEIIGKLEVVANTVIIKNEHDHILHVFPSDKFF
                     VSSSMLMRPTKSWFCRTLS"
     gene            complement(84293..>86215)
                     /gene="infB"
                     /locus_tag="PLL59_00360"
     CDS             complement(84293..>86215)
                     /gene="infB"
                     /locus_tag="PLL59_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HMY22280.1"
                     /translation="GKPVESKDGISEKDIQDKIRSTMAKLQGTKTKSDRSKYRKSKRE
                     DHAARTEGDDAQDHILQLTEFITVSELAGLMDVTPTQVISSCMGLGVFVSINQRLDAE
                     VIELVAGEFGFEAQFINLADEEDEDEEDIDAPEDLKKRPPIVTIMGHVDHGKTSLLDY
                     IRSANVVAGEMGGITQHIGAYEVETTNGEHITFLDTPGHEAFTAMRARGAKLTDIAVI
                     VIAADDAIMPQTREAISHAQAAEVPMIFAINKMDKEGANPDNIRNQLSQMNILVEEWG
                     GKFQCQEISAKKGINIDKLLDKIVLEAEMLDLKANPNKLAVGSVIEAQLDKGRGYVST
                     IMVQEGTLEIGDFVVAGQYYGKVKAMTNERGVRIVKAGPSSPVQVLGLLGAPTAGDKI
                     KVYADENEGKETANRRATIIREQGMRAKKHITLDEIGRRLALGNFKELNLIIKGDVDG
                     SVEALADSLLKLSTEEIQVNVIHKGVGAISESDVLLASASDAIICAFQVRPSMNAKAL
                     AEKEDIQIKYYNIIYDAIEEIKAAMEGLLEPKFEEKIVGNIEIREVFKVSKVGTIAGC
                     YVLDGRVARSNKIRIIRDGIVVHSGELDSLKRFKDDVKDVTKGYECGLKIKNYNDIQV
                     NDIVEAFEQVEVKRKL"
     assembly_gap    86216..86315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<86316..86861)
                     /locus_tag="PLL59_00365"
     CDS             complement(<86316..86861)
                     /locus_tag="PLL59_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22281.1"
                     /translation="MSEVKTHRLASAAKELNVGTSTIVDHLHAKGFKDVANSPNTKLS
                     EEQYEILLREFRSDMVAKEKAEAINIGKRKEEEISIKEAIKETAKVEKEKEEIIAVKH
                     EVEGPKIVGKIDLDKPKKKKEAEVKPAVEPVVEKTTEKTEPIVEVKEEKEQPEEKAQQ
                     EEKKIEPVEEVKKEEVKPVEEE"
     gene            complement(86950..88188)
                     /gene="nusA"
                     /locus_tag="PLL59_00370"
     CDS             complement(86950..88188)
                     /gene="nusA"
                     /locus_tag="PLL59_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HMY22282.1"
                     /translation="MNSAELVDSFSEFKDFKNIDRPTMMRVLEDVFKTLLRKKYGSDQ
                     NFDIIVNTEKGDLEIWRHRMIVEDGKVEDRLAEVEISSAKTIEPDMEVGEELYEAITI
                     ESFGRRAILAARQTLISRVLDLEKDEVYKKYKDRVGDIITAEVYQVWKKEILLLDDDG
                     NELILPKNEQIKTDFYKKGDSIRAVIKNVELRGSTPLVILSRTDGSFLAKLLENEVPE
                     IMDGLIVIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDI
                     INFTNNVNLLLQRSLTPAKIEGIALDDAKKRASVYLKPDQVSLAIGKGGFNIKLASKL
                     TGYEIDVFRDTDTVEEDDVDLDEFSDEIDGWIIDILKGIGCDTAKSVLELDRDELVRR
                     TDLEEETIDDIIKILKTEFE"
     gene            complement(88194..>88721)
                     /locus_tag="PLL59_00375"
     CDS             complement(88194..>88721)
                     /locus_tag="PLL59_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22283.1"
                     /translation="RKLNGRGLDKVLFFILIAMEKLVQLKQLIENEIARYFENNDLFL
                     VDIKVLSNGKIEIYADSTNNITVEECVQISRHIQHFLEENNLMTDNISLDVSSPGIDE
                     PLKVAQQFQKQLNKQVDVVLKNGMKITGELLSATSNEISVKEIIKVKKSETIEEHTLA
                     FDEIKSVKKHFNFKL"
BASE COUNT        26734 a        15916 c        15046 g        30925 t
ORIGIN      
        1 gaatatatca ttggtttttc tgtcgtagaa gttataacct gctaatacag acaagtttac
       61 atttttcacg atttctttat tgaaagcagc tgtgatatcc aatgtaatat catttcttct
      121 gttaacttgt tctgtatatt tatcagcatc tgttgggtta gcaccaccaa tagcgtagtt
      181 atatttagga cggctgatgt agctgttact tgtagtaaag ttagtacctg ctcttgccat
      241 taatgaaata tagcttactg gtttgtattc taattctact gaacccaaaa cgttatctgt
      301 tttattatca ttatttgtat tatccattaa ccagaatggg tttggtgtat atccaccata
      361 gtagccatta aaatcttggt agatactgtt gtagtctctc acttcactta aaggaatatt
      421 tactggcgtt tggattaata tttggtaagg acctacgaaa ccaccaccta atgcttgacc
      481 ttgtatagta cgttggttga tattagagta agctaagttg aacttagtgg taactttatc
      541 gcccaatttt gcagatgcat ttgcagtaat gttatttctt ctataaaatg tattagggaa
      601 aataccttta tttctgatat taccataaga cacgtagtat gtgaatttat catttccacc
      661 ttctaatgct aagttgctag ttaccgtagt acccgtattg aaagcatctt ccaattggtg
      721 ttttactgca ctgtaaggac gaattaactg tgagaaacca ttaggagtgc tcacggctgg
      781 tgtccatgga cgaacaacgc catcaaattt tggaccccaa gagaagtttt cgattggatc
      841 tggaccattg tcataacctt gaccgaattg atcttgacgc ttcattaata aatatacttt
      901 atcaaatgta acgccagagc ttgctgtaat attgaatttt ttattgtctt ttaaaccttt
      961 accactttta gtagtataga taataacacc agcagtacca cgagaaccgt aaagcgctgt
     1021 cgcagcaggt ccttttaata ctgttactga ttcgatatca tcaggattga tatcatttgc
     1081 tctgttacca aagtcaacga agtcacttcc tgaattacca taggagttcg tgttgttatt
     1141 gattggaata ccatctacaa cgattaatgc gttgttacct cctgttaaag aagcttcacc
     1201 acgcaatacg attctagtag aagcacccac ttgaccaccg attgtgttga ttcttaagcc
     1261 tggtactttt gattggatag caccaattgc attagtagaa ccactgttga aatcttcact
     1321 agtgaccgtg ttgaatgaac ccgtaatact tcttgactct cttttgatag ccattgctgt
     1381 aactactagc tcttctaatt gtttgccctc aacagccata gaagcatcta tcactaaaga
     1441 aaggattggt ttctcaaggt tggtgtagcc aatgtaagag aatactaatg ttgttacatc
     1501 ttttggtagt tccaatgtgt acttaccatc gatgtctgta accgtaccga ttgtggtacc
     1561 tttacctgta acgttcacac ctatcagcgc ttcgcctgtg gtcgcatctt ttacagtacc
     1621 acttagcgag cggttctgcg caaatacttg ggtcgttagc aacagcccaa ataaccataa
     1681 taataacgtt tttttcataa agactaactg gttttgttaa taattcttaa agaagttaat
     1741 gtatgttaat ctgcaatatt actacatttt tttttagaaa caataatttt taaagaaatt
     1801 ttaagctaat gttatctgat aagactaaaa atacttgttt acagttgcat gaaaaccata
     1861 aaatactagc gttttagcgc tgtaagtgtc ataaataatt ttatgtgtgt attaatttta
     1921 ttgatgtagc gcaaggtttt tagtagttta ttacctgtac ttctacatct tctggaagag
     1981 ttctgaattc atattgctga ttgcgtaatc ttggttcaaa acctgcatca agtatagcct
     2041 gttgtatgct tttagatgta aatcgatgtg gtgcgccagc tgcagacact acgttttctt
     2101 caatcattat agagccaaaa tcattagcac cagcacgtag gcacatctgt gctatatctt
     2161 ttccaacagt gagccatgat gcttgtatgt tttttatatt cggcaacatg attctgcaaa
     2221 ttgccagcat tctgatgtat tcatcacctg ttacctgatt gctgatattg cgcatttttt
     2281 taagcaaggt gccttcatct tgatatggcc aaggaataaa ggctaaaaag cctttagctg
     2341 aagctggttt ttcgctttgc acttgacgaa tccacactaa atgttccata cgttcatata
     2401 ttgtttcgat atgtccaaac atcatggtag cagaagtagt gatatgcaat tgatgggcag
     2461 cgcgcatcac gtcgagccac tcgcctccac tgcatttccc tttagcgagc aaacggcgta
     2521 ctctgtcatt taatatttca gcaccagcac ctggaagcga atccaagcct gcttctttca
     2581 gcgatttcaa tacttctgta tgcgacatgc cttctaactt gcagatatgt gcaatttctg
     2641 gcggacccag tgcgtgtagt tttatgtgcg gaaataattt ttttattgca gaaaaggtgt
     2701 ctttataaaa ttgcaaacct aattcaggat gatggccacc ttgtaataat aattggtcac
     2761 caccgtagcg aatggtttct tcaatcttag ttttataagt atctatatct gtaatataag
     2821 attcttgatg tccaggtttt ctatagaaat tacaaaattt gcaattagcg acgcaaacat
     2881 ttgtcgtgtt tacgtttcta tcgatttgcc atgtgacaat attatcaggt ttttgaatga
     2941 gccgaagttg atgaccgatt gccatcagct ctgatgtagg cgcattttca taaagaaaaa
     3001 ttccttcttc aatactcaga aattcaaagc gaagtgcttt ttgatataaa ttagaaagcg
     3061 tcatttttca cttacattta aaaacaaagg taaaggtaaa tgattaatct acaaggttaa
     3121 actattgtat catattggaa atggcatatt aaaaatgaga acaaggctta tgctttgtgt
     3181 tatgtataat ttatcaattt aaaaaagtgt attttcgtgc catgattgaa tattctaaag
     3241 aagttttaaa aaacggatta acacttatac atcattacga caacagcaca ccttttgtgg
     3301 ttgttaatac attatataaa attggtgcaa agcacgaaga ggaatcgaga actggatttg
     3361 cccatttgtt tgaacactta atgtttagtg gcagcaagca tttccctgat tttgacaagc
     3421 ctttacaaga agctggcgga gaaaataatg catttaccaa taatgattat accaattatt
     3481 acgacactgt gccagcaata aacattgaga ttccgctttg cttagaagca gaccgcatgt
     3541 cgcagctcaa tatcaacaaa aaaagcttag aagtgcaacg caaagtagtt tgcgaagagt
     3601 ttaaagaaaa ctacatcaat cagccttatg gcaatgtatg gcatttattg cgcgaaatgg
     3661 tttatactaa acatccttat cgctggccaa ccattggcaa atctttacaa cacattgaag
     3721 aggcgacatt gaatgacgtg caaaactttt acgatactta ttatcagccc agcaatgccg
     3781 tactcgtcat cgctggaaac attgagcaag caaatgctaa agcattaaca gaaaaatatt
     3841 ttgcaagtat cgaaggaaag ccagtaagca ataagcaatt tgaagaacct gagcaaaatg
     3901 ttgccagaaa aaacaccgta tatgaagagg tgccacttaa ttccatatat atagctttta
     3961 aaatgtgcga ccgattgcat cccgattatt atgttgcaga tgtgtgctct gatgtgttgt
     4021 cgaatggtcc ctcatcgcgc ttacaccagc gtttggtaaa agaagaaaaa gcatttgtag
     4081 aaattgatgc ttacatcact gccagcgacg atattggtat gtttgtattt gaaggtaaaa
     4141 tatctgaaca cactagtatt gaaacggcag aacaattaat ttggaatgaa attgaaaaaa
     4201 taaaacaaga atctgtcaac gataaagagc tacaaaagtg caaaaataaa atgttgactt
     4261 atctaagttt ttccgaatca aatttactca acagagccat tcacttagca gcatacgaag
     4321 cgctaggaag tattgactta atcaatgcgg aagaaaaaaa ttatgatgct gttactgccg
     4381 agcgaataag cacttttgca caacacacat ttacaaaaga aaaaagcaat actttgtatt
     4441 atttaaagaa ataaaatgac caagcctact attcattcca ttgaaaaaat acactttcct
     4501 actccagaac atattgtatt atccaatgca atttcgctat atggttttaa tggtgcaaaa
     4561 aacgacattt tgcgtattga tgttttattt aattcaggtc gttggactga gccagcaaaa
     4621 ctgatagctg aaagcacttc taaacttttt aaaagcggaa ccaataattt gtcttccttt
     4681 gaattaaatg aacaaattga tggctttggc actactataa aagcaagttc tggatataat
     4741 acgttcaccg tgtctttgta ctgcttacat agatttttag aaccttcact acaattatta
     4801 cgcacgtgct taactgatat tatctttcca gagaacgagt tagaaatttt gcaaaaaaat
     4861 gcgctttcta aactcaaaat aagtcaagaa aaaaatgact atttagcaga tgtggctttt
     4921 aagaaaacac tattcggcga agaccatcct tatggttatg agactacaga aaccgccatt
     4981 aaaaacattc attctgcttt gttaaaacag ttctacgcaa cagacataac accaagtaat
     5041 tgcacagtac ttatcgcagg aaaatatgca gcaaaagaac tgcaattaat tgagcaatat
     5101 ttaggcagct ggcaaagtag tattcaaaaa caatcacctg ctcaatatca aaccttaaac
     5161 gtgcagcaac aatgcttacg cattaagaaa gaaaattcgg tacaggcttc tattgttatc
     5221 ggcaaagaga gcttcaataa acatcataaa gattatgcct cttttgtatt attaaacacc
     5281 atatttggcg gctatttcgg ttcgagatta atgagtaata ttcgagaaga aaaaggatta
     5341 acctatggca tctattcagg cttgtctcct ttaaaatatg gtggcatctt ttcgatacaa
     5401 accgacacaa atttagagaa tgttgacttg tgtttgcgtg aaatatatgc agaaatggag
     5461 cgtttacaaa cggagttgat tagcgaacaa gaaattgtgc tggcaagaaa ttatttatta
     5521 ggaaaatttt taagcagaac cgatggtccg tttcagcaaa ttgaagtatt taaaagttat
     5581 ttcatagaag aagtagctat taacaggttt gaagaatttg tggaaacaat tagacaaaca
     5641 gatgcattat ctttacaacg attagcaata caacatttgc aaaaagacag catgaatgaa
     5701 attgttgttg gataggaaat ttgaaataaa tgtatttgaa atgaattggc gagaaacgat
     5761 actgaataag atagtaaagc atgtatttac tgcatattgc atattcatta cggcatattc
     5821 cttttcacaa acagaatttg aagcgccaaa gctaacttgt gttcgtaata atgttggcga
     5881 tatcgaatta cagtggcaac tgccttcaag tgtaaatcct tgttttgtcg cttatgaaat
     5941 ttatgcttcg attggcagca agaatgggcc ttataccttg aacacttcaa ttactactgc
     6001 ttcacaaacc actgcactat taacaattgc tagcgctggc cagcctgttt ttttttatat
     6061 gataaacaga ggcagctgca acaatccaat accattagca aaaaaaacat cagacacatt
     6121 agacaatatt aaaccacagc catttgtaca actacaaaat gcaacggtga ttaacaatca
     6181 agtgcatcta cattggctcg cagcagcaag ccctgaagtg agtgcctatt tggtgtataa
     6241 tgatcgtgat ggcttcactt cgccagatac tgttttagga aggttaagca ctagctatgt
     6301 cgatatcgtg aatgatccga gccaatttgc tattcgttat aagatacgtg cattagaata
     6361 ttgcgaagat gttgctggtt tacaaggatc cattacacca gacagcgccg accatagaac
     6421 tacgctatta caagtaggtg ctgctgacaa atgcacgcaa acagcaaatc taagttggca
     6481 gatgtataaa attggtagtg ctcaggttgt ttcttatgat atcgaacaaa gcatcaatcg
     6541 cggtgctttt acgacagtag gtacacagcc aggcacgtcg caaagttttt tgttgcaaaa
     6601 tattccgttt agagatacca tttgcattcg tgtgaaagcc aatttgccaa atggcacatt
     6661 tgcatactct aatgagcgtt gcctaagtgc tgatgtcatt aaaagaccag aaaatgatta
     6721 tatcagaaac attactgttg aacatggcga tattattatt gaatatcaca aagacactac
     6781 ggccgctcct gcaagaacag tgattttgca acgcagtaat gatggcatta ttttttctcc
     6841 tacattaact gttcctacag agcctgaccc aaacacttat ttatttacag aacctaattt
     6901 atctgtgcaa aaccaaacat ttacctatcg tgtaaattta ttagacagtt gttttattac
     6961 gcatacttct gacacagcga caactattcg tgttggtatt aaagtaaaat caaataataa
     7021 agctgatatc atttgggctg gttttgaaat aaacaatatc acattcgacc attttttagt
     7081 ggagaaaatt attggaacag atacaagtgc tgtaggcact ttcaacagaa accaaacgac
     7141 ttatgcagaa actgcattgt ttgattatac tgctgattca ttacaagaaa cttgttacag
     7201 aataacggca ttttttgaaa acaataatga tgttgctccg cgcgaaacat taaaaagtca
     7261 ttctaatatt gtgtgcgtac agcctacacc aaaagtatat gtgccacaag cttttgtacc
     7321 tacaggccat aacaatactt ttaaaccttt tttattgtat gccaaagctg atgcttatga
     7381 ctttagaatt tatgacagat ggtatcaatt aatgtttgct actaacgata taaatgaaag
     7441 ttgggacggc acatttaaag gtgttaatgc gccattagat ggctacatct acatcattaa
     7501 atttaaaggc aaagacgata aggattacac acaaactggc accgtaatgc ttttaaaata
     7561 agcttggaat aaagcaataa aaaaatgctg atttctttat tcttttaaag acttctttat
     7621 atatttattc atcttgaact aaatacacat taacctataa tcatattcat gtcaaataat
     7681 ttgcattcgc atatattgat gatgtgatag gcagctatat ttatgaaata tattaagcgc
     7741 atttttcatt attatttttt ggagccgagc aatgcggtgt ccgtcaacag acctgctgta
     7801 attattggcg ttttactatt aactattgca ggcgcaagcg tgtattataa tatctacgat
     7861 tacatggaca tagtgtatgg cgatgaagtg atatacatga aaaatggatt gaatattttg
     7921 caagatttta accgagattg gggcccatta tattgtttat ggtataaatt tttatcgctt
     7981 tttattcccg atacgattac actctattat ttcaattttg tacttgttgc tatattggca
     8041 actgtgctta tctattgtgc atttctgtcg atggatattc atccattagt agcgttttat
     8101 tttgctattt gttttatagg ttcaaaggct gtagtgccga tgtggccgcg aatcagctta
     8161 tttactattt gtattttaat gattgcgatt atttctattc gaagaataaa agcattgtat
     8221 gcaagattaa tgcttgtgtg tattgccgta ttactcgcca gctacgcacg accagaattg
     8281 tatctatcgt ttctactttc tctttttatt tttattgcat atttttttgc tggaaaaatt
     8341 tacaaaaaac catatagcat ttaccatgcc ttggttttta tactactcat tgttttacta
     8401 catcttattt tccgttttcc ttcaaatttt tacaatggtt atccaaggaa tttagcagca
     8461 ttttatcagc attatatggt caaccaattt ttcttaaata aagccacaga atttgattgg
     8521 atttattgga aagacacaca caaagctata ttctctgatg ccaatacaaa tattttcagc
     8581 atcatcattc attatccaat cgaatttttt tctcatgtgg cattgaacat taaaaattat
     8641 ttggtagaaa tagttgtgaa aaacctaagc gttattttcc cttttttggg cggcatgaat
     8701 aaactttatt ttggcggtgc cttcttggtg ttcattagct caatgctatt gttgtcaatc
     8761 agtagcatac ggcaagcatt tttcatcaca ttaaaagcgc atgcctttta tttagtcatg
     8821 ttattaattt ggggcattcc tactattctc tcttctattg ttatttttcc gagagaacat
     8881 tatatcatgc tcaactattt tctgtttatc attccattgt gttttttatt tacagaagcg
     8941 gctaagcaat tattgcccaa tgtgtcttta aaacatttta tcatttttgc aattgttctc
     9001 attccatttt tatcttcctc caaaaattat catttctttt taacagacca tgacaagaac
     9061 aatatgtgtt cgcgcaaagc attacagcat ataaaaaaca agcatcttga ccaacacatt
     9121 caatatactt tttttgctga cattcattgt ttcattggat tcttaccgaa taattttaaa
     9181 gaaataaata ccatttttga caaaacaaaa gatgtttctt tcgctacgat attgatgtac
     9241 aataaaccag attttatttt ggtcaataat tgtatcaact acagcccgag tttaatcgat
     9301 gataccactt ggcagcagtt tattaaaaat ccatatcctt tcggctatcg gtatgagttt
     9361 gtcaaagatt gtccttatta ttatttagtt cgcaacacgg ttcatgatta aaaaagagtt
     9421 caaacattta gacgccttgc gtggcattat ggcaatgtat gtgaccctag tgcactttat
     9481 tattctattt acactgaata aaaaaaacga gctcgtattt actgatttgc gatatctcgg
     9541 cattgtgcaa ttaggccaag tgtttatgac tgtttttttt gtattaagtg gatttttaat
     9601 cagctatatt ttattgattg aaaaagaaca aacagccaat gtgaatttca agaaatttta
     9661 tttacgccgt gccttccgaa ttttgcctat gtattattta ggtgttttct ccatgttttt
     9721 tttatacaaa aaagcctgta taaatcctac aattatgcag acgccaatgt gcgcagaatt
     9781 atcggagcgc agttgctact attttttatt acttccaaat tgggcacacg caatcgacaa
     9841 aactttacca catatttcaa actattggtc gattggcgca gaagagcaat tttatatttt
     9901 gtggccattc gttttatatt tttcgaaaaa ttatttgcgt tcattttttg gaatatatat
     9961 tgcgtattgt tgcttgttaa tcgctatggt cttagttggc aatttatatt atcacaatga
    10021 caacatcact tttattaata tcgccaaact atttgactat actcgctttg gcgcatttgc
    10081 ctttggtggc gtcattgcat tcgttttatt acacaacaca gaaaatacct ttcaaaaata
    10141 tcatctgctg atgacaaaaa aatctgtgca atggtcttgt ttcttactac cttttatcgt
    10201 ttgtatcatt ccgaatgaac atgtattatt tttaaaacat atactcgtca ttccgtgctg
    10261 tgcagtcact gtgtataatt ttgcctttga cgagcaatct attttaaaaa ttgacaataa
    10321 atatttgaat tacattggca aaacaacata tagcctttat attctcaatc aaattgtaat
    10381 tgattttgtc ataaaaatct gctttgctgc taaaataaac agcacactta tcgtctttgt
    10441 tataacgatg tttatactat ttctcggtgc tgttttctgt tatgaattta tcgaaaaacc
    10501 attcatgcaa ttgcgaagaa aatttggcta attaggaagg aattttcaca tcaaatgttc
    10561 ttctttcttt atttaataaa ttaaaattgc gtatccaagg attgtagatg cgtagcattt
    10621 tgtagctggt attattttct ttggcaaagc ttacccaatt aataggacca gaaacctgtt
    10681 gtatagtata ttttaattct gggtacaaat cttcatttgt caaataaaaa ccataatctt
    10741 tttgatgttg atgtatgatt ttcagtgcga caattctttg tacataacga ctcgtttctg
    10801 tatttaaaaa caaatcatag tatgccgttt cgccttgcaa tagttgttgt ttattgacac
    10861 cagctaatcc catattatag cctgcggcag cagccgtcca agagcctgtt ttttctttag
    10921 catctcgcaa ataccgacat gccgctcgtg ttgatttttc caagttataa cgttcatcta
    10981 catcactatt gatgattaag ccgtattgca agcctgttgt tttcataaac tgccaaaagc
    11041 cagatgctcc tgctgaggaa atggcattat ctaaggcact ttctgccatg cataaatatt
    11101 taaaatcatc tggaacactt tcttctttca atattttctc aattgttggc agccatctat
    11161 tggcacgttt aagcataata atggtagaag attgccaata tgcatttaca cttagctctc
    11221 tgtctaatcg ctcgcgcact tcgatgtcat ctagcggcac gttctcgcca gcaaaactta
    11281 cactttccgg cagttttacg gcatacgcat tttgcaaggg caatgctaaa ttttcttttt
    11341 tatctgctgc ttttatgaaa ggaaaatagg tgtcttctcg agtggtagca ttgataaaca
    11401 acattacaaa tagtatcgaa atgcctacaa taaaatagaa tgtgcgtttg ttcttcatag
    11461 ttctttgtaa tagtgaaaaa tgttattgca tttttacttt gttcaatgct aaatatagct
    11521 taatttttct ttttataaac cggtactgtt gagcaagcct caccgaacat aagcgattta
    11581 acgacaggtt gtaatttttt gctgatttcg atataggcat cgccttgtat tgcttttgtg
    11641 ccacagcctt tgatgacaac cctagcgtct ttatagcttt ctatgttcat gttttttata
    11701 ttatgtatga acaaaaggta ttccactttt tctgtattac caaaatatat ttcatgtgta
    11761 tgagcgcaca aataggaaac aactagcata tacgaccatt gcggcagtat ggcgtcggtg
    11821 ctacaatgca aggcgactat tttatcttta tatatcgacc aatcttgttg ttgcaatgcc
    11881 gtcctaaatt ctttttcttt taaaagaagt tcgcgaaata aataattctt catgtcaaat
    11941 tccaaaatcg gattgttggg aaaaaacgtt tccaagtcca agcttattag accgctttct
    12001 gctactttat tgattaattt atctgccata catagacaat atacaagatt gttttaaaac
    12061 aaaaaaagcg gaaaatagtt tttccgcttt tttatttatg aggatacaaa ttagtgctta
    12121 ataaaatata taacgcgtgt cgttcacatc agcttttttc aagatagact cgaatatagt
    12181 ttggaaattc atacgactga taaacatacg ttgcatttgt tctttataca tattgatatc
    12241 gcctgacatt tctggtgcat tagcgtcgat ttgttttggt tgcaccataa atgcagaacg
    12301 ctcgccagcc actgctttcg acgttttgcc tacggtgata ccaaatgtag tgcctactaa
    12361 ttttggctcg ttgcctaagc cttgtacgaa tgctgacgac atacgtacta atgctgtatc
    12421 tgtcactgcg gcatctttca cttttgctgc aattgtggaa agatctgttg tgcctgttgc
    12481 tgctttatct aatgctgcta ctaagtcttt gccttttttc tcattgcgca caagaattga
    12541 aatttcttcc cgcacatcag cagctgctgg caatcctttt ggaattattt tgctcaattt
    12601 agcaagcata tatttgttgt cattatcaaa atcggcaatt acgccaactt tttcttgttg
    12661 gaatgcccat tgaaccaatt tgcgtgctgc accaacttgt ggcacttcca ccatattttg
    12721 tgttagcaca acattttttg ctaaattttc tgttttcgct gctttgtcaa atgcttccgc
    12781 agtttgatat ttttgttgaa aagatgctgc tttatcgtac gccatttttt ctgtctcatt
    12841 acttggtgct aattcaacaa caaaatctgc aaatttggtc aacaattttg gtgtttttgc
    12901 atcaattaat aagatgatat gcaagccata ttgtgtttct acttttgcaa tttgacctgg
    12961 tgccatacca ctataaaaca ctttatcatt aaactctttt accatttggt ttctagtaaa
    13021 ataacctaaa tcaccacctt tgtctttaga gtctttgtct aatgatgttt caacggctac
    13081 ttgtccaaag tttttcgcac cagactgaat aacgcgaatt aaactatcag ccaagttatt
    13141 tgctgctttt ttctctgctt ctgtgccatt gccttctgat aataaaatat gtgcagcacg
    13201 aactgaatct ggcgctgatt ttctgtcaat gattttagta aacaacaaga atgaaccttc
    13261 gttatataca ttcgttaatg tgccgacagg caaagaaaat aaagtgtctg cgcttctatt
    13321 ggtttgcatt acttcgtcct ttgaaaaata gcctacacca gctccttgtt gtgagttgcg
    13381 tgcaataaat gcagagtcgt ttggtaaaga tgcaatatac tcttcgcgca tttttaatat
    13441 tttggctttt gcggctgctg tgtcttctgc tgaaggaata atatcaaatg atacaacgtc
    13501 aacaacacgg ctagcagctt gctcaaattt tgcggcattc gctttcaaat atgctgtaat
    13561 ctcatcatct gttactttgt atttatcatc agataatgat gcatacggaa cgcttacgaa
    13621 agcgactttc gcacttctac cagagttgac aacttctttg gccataaatg ttggcatata
    13681 aaaagattgc gcaactaagg aagaatattt ttgtttcacc atgtcgtctt ttatcaagtt
    13741 ttcgatatgg ctcaacatct gacggaagtt cgttttattg tctttgtcga ttttatcgat
    13801 gtttgtgata aactcacgaa tttttgcttg gtttaatctg ccagtttctt ggttgatgac
    13861 atctggacta acttgtctga tttgcattaa aaaacgttgt gctaatggat gaggttggtc
    13921 gccccacatt aactcgccca tttctccttc tgaaacggct aagcctaatg atgaataagc
    13981 atcacctaat aatttttgtg cagcaatggt attccaaact tcttcttgaa tttgcgattg
    14041 caattgctca tttaactgaa gatttggatt aattaatttc aatccttctt cgtaattggc
    14101 gatacgttcg ctgtattctt ttccagaaat ggtagaacca ttaatttttc caacagaatt
    14161 tttaccacta ccaccagaaa acaatgaatt tctttctaat gctgcttcca caataaaaat
    14221 aagtaatgcc aatgcaatga atcctattaa tatccatgaa cgctgtctga tttttccaat
    14281 taatgccata atatatgctg atttttaaag agtgcaaaga tagcagataa tttggaattt
    14341 tggaaatgta tagcaggaat aagcactatt ttttaagcag gaggaagaaa gctagcttat
    14401 tgattgtcag cactttcatt gatttcgagc tttacggttt caattttggt ttctgaaacg
    14461 tctatgattt tgaaactgta tttaccaatt tcgatgattt cattcttttc tggaatagtt
    14521 tcatgataat cgataatgaa gccagaaagt gtgtcgtaat cgccttctgg aaggtctaaa
    14581 tcatactctt cattgatgcg atctatttct aaacgacctg ataaaataaa ctcatttttc
    14641 gacaattgat tgccgaccaa ttcatcttta tcatattcat cttgaatatc gccaaaaatt
    14701 tcttctaaga tatcttccaa ggtaattaca cctgctgttc caccatattc gttcaccacc
    14761 caagctatgc ttttattttc ttttataaat tcgttgagca atttttggat atgcatcgtt
    14821 tcaggcacca ctttaatatc cattaatatc gaacggatat tgcttggttt tttatgtaaa
    14881 tcaaaatgat gaatataacc tacaattttg tcgatggagt cttcataaat caatatgcga
    14941 gaatggtaag attcgatgat tttctgcttg agttcttcga cagaatctga aatatctatt
    15001 gcggtgattt cgcggcgtgg caccatgcaa tcgcgcacgc gaagtttttt taaatacaat
    15061 gcattttcaa ataattctgt gtccacttct tcctcttctt cactatttgt ttgatgatga
    15121 tctttaataa atttttctaa atctactgaa gaaaattcta tgtcttgttc tgatacttta
    15181 atgccaaata ttttaaacaa aagcgtggaa atgccagtaa ttaaaagcac gccaggtgtt
    15241 aatatccaat gtataattct aaatggtaat gccattaatg gcaatatact gtcagcaaat
    15301 actttaaata atacttttgg aacaaattcg cctaaaaaca aaattatagt ggtagaaaca
    15361 aatgtttgaa tacttaaaat aagtagctca ttcgtgagat attcttctaa tattggctcc
    15421 atcaacatcg cagttagaat accaaatgat attaatgcaa tattatttcc aagtaaggta
    15481 gtaacgataa atttagatgg atttttaata tagtcattga gtatttttcc agacagtgtt
    15541 ccgcgttgat aattcaactc tattttgagt ttgtttgctg ataaaaatgc gatttcaatt
    15601 cctgaaaata tagcagaaaa tatcagtgtt agaaaaatca atattaatat ggtgcttgtc
    15661 tccattgcgg tataaagata aaacaatagc gtctataatg tatgctaatt atcgtttgtt
    15721 ttgttggcaa caatataata taagccacca attaacacca tcaaaacaag atacaatata
    15781 aaatcttttc catgaaaaaa cccattgttt acgatacgta tgacaacgat accacacatc
    15841 ataatggttg tgattaacca aattagcttg ttcttattga aatccttgtg cattgcttac
    15901 ggcaacaatt ccgctgacct tgcggatcgt gtaattagaa aaatcttcat cggcatcaaa
    15961 gcctacacca taaatggtat tgtctggtgt tttaattttt attggtttgg aggtaaatat
    16021 ttttttgttt cgaatgtccc aagtcaactc ctctgtttcc aaaatttcat atctcgaatt
    16081 ttcgatatac actttgcctt tgatatacac tttctgttct tcatcgttat tctctgcata
    16141 gtttgccttc attacagaaa tcaaatttag cttgtcataa aacttgacca ataggccatc
    16201 tgtaaattca agtctgtttt tagaattagt gaagcgtttt acggtttttc cagtaacaat
    16261 tgctctgctg tatgctcctt ctttataggt aaattttacg ctgtcggcaa tttcaacatc
    16321 ggcatctgtt tcattaaaca atgctttgac tttggctatg tcattttctt gacacgatga
    16381 aaaaattagc agcatagcaa ctaaagtaag caaagcaata ctatatttag gttgtttggc
    16441 tgcacttttc atcattacaa cttttgtaat tattttttat aaatccaatt ggaagggtcg
    16501 agcttggctg ctcctttcca tacttccaaa tgcacttccg taatgttgtc ttcatccgtg
    16561 tatgcggtgc cgatgacttg ttttgttgaa attttttgcc cttttgcaac attaactgat
    16621 gctaatttag aatataccgt aaaatattcg ccgtggctga tgatgactgc atttttgaat
    16681 gttggattat ttaaaatgcc gactactttt ccatcaaacg cactgcgcac actactattc
    16741 gcatcggtgc gtatgtcgat gccattgttt tccatcacga tggaagagcc tggcacagta
    16801 tattttccga aacctttgct aataaatcct ttttcgaccg gccaaggcag tttgccttta
    16861 ttgccataaa aattagaaga taattggtcg tattctggtg tgttgcctaa ttttactggc
    16921 gtttccgtta cttttttatt taccgttgca atatcttctt tcgacggctc tttgccggct
    16981 tctttcgctt ttttgattgc ttcttccgca gcttttttct tagcagcttc tgctgcagcc
    17041 aattttgcag ctgcttcttt ttgttttttc tcttctgcta atcttgctaa ttctatttct
    17101 tttttgatga tagcctcaat ttgtgcatct aattttttcg cttcagattg ttttttagtg
    17161 atattggtct gtagttcttt ttcttttttc ttaagcgttt gaacgactgt atttttttgc
    17221 gagactgatt tattaagttg tactttctct ttgacttgtc cttgtagcaa ttgttctttt
    17281 gtttttttct tcgcgtctat attggcaatc tgcgtgctga tatccgattt tttatctatt
    17341 atttgtgtcg cttgttgctc tctgaaagtg gcatattttc tcagataatt aacacgacgt
    17401 agtgcttctg aaaaagaatt tgcattgacg acaaaaagca atttatcggc aaaacgttgc
    17461 gatttgtaag tgcgcacaat ggcatctgca tattcctttt ttagctttgt caattgtgat
    17521 tctttttgtt tgagagaatc gacattttgg tttatttttt ctgtaaaatc accaatctcg
    17581 ccttctactt ctttaatgag tttttggcgt aattcaattt gttttgacaa ggcttgtaaa
    17641 tctgctaatg tggcgtcttt cgatgctttc gttttggtca atactttttg tgtaaactcg
    17701 atttcatcta aaagtttttt cttttttgct tctaatgttg cttttttatc ttgagcatta
    17761 acataaaaaa aacacgacaa taaaaatgtc aaaagcacag tttttgtaat tgtattgctc
    17821 aataaaaagt agacagagga agccgagtat gtcgagttat ttttcaattt tatagtttgg
    17881 atttatggta aatggatatt ccatattttg tttcacttca atactttgga ttttagcaat
    17941 catctcaaaa gttgatgtat tatttttcat tttcaattct ctatccatcg aaaatggctt
    18001 tcctaattgc atttgataat tatcatattt aacatcaatt gtttgcggat actttttatc
    18061 acttacaaaa atagatgttg gatttttgct tgttttatca aataaaacat tagtgaggaa
    18121 gcgaacacca tcggcattga ttgttgtatt tgtagcattt tctacatatt tggcttttgt
    18181 tgaatctaca aatactagtt gcccgagcaa taaattttca atatcggcaa aagacaaatc
    18241 aaccaatgtt ttttgtttaa tatacgacaa tggttttaag acatatctgt tgttaatttt
    18301 gtccataatt tttatactgt cttttgtaat cagtacgcgc gctccttcga tgccaataat
    18361 agacattgac agccaaattt tttcattttt tttccaacgt atgttagttg tgaaactctg
    18421 attgaggtct ggtgatgtta cttttgtttt taacttaagt gctaattctt taaatacaat
    18481 ttcattgtta ttgacttttg ccaataatga cgacacggcg gcatctatct ttattggtgt
    18541 tggtattgtt ggtgttggtg ctattggttt tataggaaca cttactatct catctgtttt
    18601 ttttattttc ttatttctgt gacaagcagg caataacaac aacatgctca taagcataaa
    18661 gagcaatgtt gttattttga ttttatagaa tgaaaaatta gccatcattt cggaatatgg
    18721 ttatttttag aaacaagtat gtgatttaat taattttttg atttttgatt ttcagttcta
    18781 gcgcaatttt atcgccacct ctttgcaatg ctttttccca ttgcaaaacg gccttatcca
    18841 cttgaccaac tttaaacaag atatcaccgt aatgtgtaag caattcagcc ctttcttgag
    18901 catctggaaa ttccatagct tgttcaatcc atgttagcgc ttctgtataa tttttcattt
    18961 tatacataat ccaagcatag gtatctaaaa atgctgaatt attatcgacc agtaagtttg
    19021 attttttgct catgcgttct gccttttcca gtctgtcgtc acgctctgaa agataatagg
    19081 cataattatt taacaccgtt gcattatttg ggtctatctc taaagcggct tcataggagc
    19141 tatcacaacg tgtataatct ttcaattcga atgacacatc acctaataaa atcaacattt
    19201 gtaattttag ctgaggcgtg taaattggat ttgatgtaga tttttgttgc gtggcaaacg
    19261 cttgcaatat ggcattgctg gcttctttat tatttttctt ttgttgctgc gcaatcgcat
    19321 tatagaaata gccgaaagga tcattcggat ttttttgaat cccttccttt gtatgctcaa
    19381 tcaatttgtc ataatcttcc agctctgtat ctaaaatata gagttgtatc caaaccgtat
    19441 tgggaatatc tgcaccaagg tgtatcgctt ttacatattc attttgtgca tctcgctttt
    19501 tgttgctatt gtataataca tctgcgcgag ctgtggttgc tttcacgtca tttggatgcg
    19561 cctccaccaa tttatctgcc attagcatca cttcattgcg catgctagaa tcttttgcca
    19621 tacgctcgat atatggtata aatgccatta tcttggtgtc gatatcgata tttggattat
    19681 taaatacttt gattaggtat tcatcgtgtg ttttattgtc gcccttttgt ttatatatat
    19741 cagcaattgc cagtaacgca tctgcattat taggctcttt ggcaataatc ctattgtaag
    19801 tttgcaatgc tttttcatac attttattcg acagatagag ttcgcctgcc aaggcaatat
    19861 aacgcgtatc cgaaggatag agtttgacga gtttttcaat gtctttgact gcatcttcta
    19921 tattgccaag tttaatatac agtggttgtt tttgaaaaat aacttcttct tgaacacctg
    19981 ttttttgttc taataaatca tatacttcga tagcgtcttt atacttttct gctttcgtca
    20041 gcatataagc ccaatcgtag taataatcgt attcttttgg cttcaattgt attacgtttt
    20101 catacacttt ggcggcgccg ataaaatctt ttttttctcc ttttgcttct gctaagtatt
    20161 gataatagaa ttcgttattt ggatttagct ttattgcatt ttgcgcaaat tcgatggatt
    20221 tgtccatatt tcctgcttcg aacgcaattt tggaaagctc atataaagct gcatcgtttt
    20281 tagcatctct gcgtaatact tcattaaatt tagtaattgc atcttgcgaa tttcccaaaa
    20341 tataagcttg catgccttct ataaacaaag cttgtaattc taagtctttt ttagtggtaa
    20401 cagtagtaga tgaggaagaa ttatcttgct tgttcttttt gtttttttta gcaaaacttg
    20461 atggtgcaat acttagcaac aacacaacac taaatagata tatcgatagc gtatttttca
    20521 agatgaggtg tttattttac atgaataggt gtcaaaattt gttcctttct tactaatata
    20581 acgattttag cacgcaaaca gtgacaactg tagaagaatt aacctttttt agcaagcctt
    20641 ttatttattt gacgcctgta tgtccaaagc caccttcgcc tcttttggtt tcattaagtt
    20701 catcaacttc tttaaagcta actttttcat atttggaaat gattagttga gctatgcgtt
    20761 cgccagtatt gatgatggtt gtttcgttgg acagattaat tagaataatt tttatttcac
    20821 ctctataatc agaatcgatt gtgccaggtg aattaagtac ggttacgcct tttttcaatg
    20881 ctaatccact gcgtggtcgc acctgcgctt cgtagccatc gggcaactcg acaaataatc
    20941 ctgtcggaat caaagcgcgt tgtagtggtt taagcatgac aggttcttgc aattgcgcac
    21001 gcaaatctac gccagcagat ccttctgttt catatgttgg tagtggatta tcggaagtat
    21061 taactatttt tagtttcatc gtttttagat atgcaaatta gaaaatatat tgcccaagcg
    21121 cttcgtcgct cataaacacc atacaaatat agtgagattt tttattaacg acttattttt
    21181 tcttttctaa aaagtaggca ataaacaagt aaaatagtaa tagcaatacg gcgattgtag
    21241 tattcaagaa taatgaaaac gacatcggca aaacaagaaa tttcaacaat aaataaagtg
    21301 caagcgctaa tatgatatag cttacaatgc gtttgtaatc gtatggtata ggataatatt
    21361 tttgctctgc aatatacgca atcaacacca tcgacaaata gcacaataag gttgaccatg
    21421 ctgcgccgtg gtatccaatt tgtggcacca aaataacatt ggcaatgatg gtaatactgg
    21481 cgcctataat agaaataata gcgcctatgt aggtcttgtc agtaatttta taccaagtag
    21541 aaatattaaa ataaatacct aaaaaaatat tagccatcag taaaatagga atgatatcag
    21601 cacctacaaa ataattaatg gaattaggag aatacaaaac aaaaagtgct tgtattagcg
    21661 gcaaaaacaa aacaatactc aaaaagatta cacacacaca aatcgtgtag tatttcattg
    21721 tcttcgccag catttgcttg ttatctgact tatcagcata tctaaaaaaa atgggctctg
    21781 cagcatacct gtatgcttgc aaaaacatag atacaagcat agcaaattta taattaaagc
    21841 cataaatgcc tagttgttct ttatttgtga tatcatcata aggcaataat ctagtcataa
    21901 ttattctgtc caacacttcg ttcaccatac cagcaaagcc cactaacatt atcggcatgg
    21961 catagcctaa catttttttg tacaatgcaa aatctatgcg tgattgtaat tgctgaaatt
    22021 gttttatcaa aaacaaaaaa gtcaatatgc ttgccaaaat attagcaata aaaacaaagg
    22081 aaacttttga tggcacacta ctcatcaaag tactacttat accttttgtt tccaatattg
    22141 gccacagcaa aaagaaaaaa atattaaatg ctataaacaa tgcgatattc aatattttca
    22201 aagctgcaaa tgcaattgga cgtccttgtt ttctgagcga tgcaaatgga attgctgcca
    22261 gcacatcaaa tgccatcatc cacacaaaaa actgaatgta tagcttcggt tctttgacat
    22321 attcggcaat actgttttga aaaacataag caaggattac aaataaaaca acagcaataa
    22381 gcacggaaaa aaatgccgtt gtaaatgctg tttctttgct ttcggcttta ttggcaaatc
    22441 taaaataggc tgtttccata ccatgtgtta gtgccactaa aataaaacct gcatacgcat
    22501 aaaaattggt aaatgtacca aactctgctt gggtaatata tttgataatt agaaagccaa
    22561 aaaggaaatt tattaacctc gccaatatgc tgcttacgcc atacgcagca gtgtcgcttg
    22621 ctaatttttt tactgtactc aaattatttg gattaggaca aatgtacata atttataaaa
    22681 atgtagtttc ttgtttttct ttaaatatat gcatatataa aaaccaatct attttattta
    22741 ctttgttcta tgaagtcgcg aatagcattt atcctaatag taattgtata tttcacttcc
    22801 tttttttatt acaaaagcta tcgtaatttt ttacaaggcg gtggcgaccc ttggggctac
    22861 tatgcctatt tacctgcatt atttatttat gatgatttgc atgacttaca aaaaacaata
    22921 ggcaagcgtg ctgaatacaa tgcaagcagc gtgcgcacac tcgaaaatgc ttatctgcaa
    22981 attgaggaag cacacgcatt taaacacaac acgattatca aatacacaaa tggcgttgcc
    23041 attttatata gtccattttt ttttgcggca catttctttt gtaaaagcac aaacctttat
    23101 gctgctgacg gctatacact tccatataat attgctgttg gtttcgctac aatattttac
    23161 agtttatttg gattatggat tttacgaaaa ttattatcca aatatttttc agatagcatt
    23221 agcgccatcg tgttaatgtc tattgcaatc ggcacaaact tatacttttt ttccgtgtcg
    23281 cacctcggca tgtcgcatgt gtttttattt gcactatatg cgctatgttt atgggcgaca
    23341 gattgttatt atcatagctt taaaacaaaa tatgctattg caattggttt ggcatgtggc
    23401 atgatcacat tgatacggcc taatgaaatc atcattttgc tttttccgtt tttatggaat
    23461 gtttattcta tcgatacttt tagggaaaga atagaaacaa tacgtactca tgttggtcaa
    23521 tatgtattaa tgacattatt atttttaatt gtgctattac cacaatttct gtattggaaa
    23581 attttatcgg gaaattttat cttttatagc tacacaaaag aaggttttga ttttttgcat
    23641 ccacatataa aagaaggaat attgggcttt gcaaatggct ggctgtcgta cacaccaatc
    23701 ctattttttg ctgttatagg tttgtttttt ctgcatcgta attttaaaaa ttctgcattg
    23761 ccatcattcc tgtttttacc tatatacatt tgggtaattt atagttggtg gtgctggcaa
    23821 tatatcaatg gctttggctc cagacctatg atagaaacat atcctatttg ggcgtttccg
    23881 atggctgttt tttttactgt gatagggaaa aaaataggat taaagacact ttcattggct
    23941 ttggtttgtt tttttatttt tttaaatatt tttcaaactt ggcaattcaa agcaggctta
    24001 ttgtggacag aagacagtaa ttttgcgcat tatcgcgcaa gctttttgca aagcaaagga
    24061 agctacaatg cacatattgc ttttgattct cgcgaatatc aacctgacag caataagcta
    24121 cagtttgtga aaactattca aacgcaatct ttcgatgatt cttttagcaa ttcttttaat
    24181 gctacaattt ataaaagcag tccttatgca tttgagctaa aagaagggac gacaccagcc
    24241 attcagattc cagcaaatac cgcaggtgta caagctttgg attatttcaa aatttcttgt
    24301 tgggtgtatt gtccagcttt acaatatgac cattacaaac aagctgttat tgccgctgag
    24361 tttgtacata atggtgcgca aattcgatgg cgacctgtgc gattgcaaac taaaattgac
    24421 aatcctaatc atcatttgct acatgctggc aaaactaagc aatggcaata tatatccttt
    24481 tttgtgcgcg caccacatcg atataaagat gagcgcgata tactaaaagt gttcgtttgg
    24541 aatccaagcg aggtgcctat catggttgat gatatacaga tagcactttg gaaaaaaata
    24601 caataaaaaa gaatgagccc tttatgaaag tataaatgtc tcataagtat ttgacttaca
    24661 aagatgttta taagaaatct ttcggtataa agagaaactt aaaaaagtgt accccaaagg
    24721 catttttaaa taaaaaacgc ctcgcaaact attaatttac aaggcattac aaaaattgtt
    24781 tgtggtgtgg ggcgggatcg aaccgccgac acaaggattt tcagtccttt gctctaccga
    24841 ctgagctacc gcaccgaaat catctttaca agataattgg acggcaaaga tagtattttt
    24901 tcgatgttat ccaaaaaaaa ttatggtttg tattcatata aaccattttt tagcgtaaaa
    24961 taatattcat ttaattttgt ttgctgcgga aaagtccatt ctgcacctaa aaatgccgtg
    25021 ctgtccgtca aatattcctc gattttataa aatgattctt ttaccaaaat tgttttcgac
    25081 tcaccattta aagtacttat caaaagtgtt tcatatggca ccgaccagga catcgtttgt
    25141 atgtcaggtg gcatattttc atatagaact atttgtttat tagtgccttt tgccattaat
    25201 acctttaaat attcaacgcg atggctgtat gttttttcaa tatatagact tgaaaatgaa
    25261 attaaattca caagcaatat caatccaaat atggcatttt tattttttga tattgttatt
    25321 aaattaaacg caatatacga ccacagcaat aaaaataata ctgctagata tttatctaaa
    25381 taaatggcac tagcgccatg aatcagaaat atgacaagca atagatacat gccacaaaca
    25441 ccaaatatca atgccatttt actccaaatt tttgtttgaa tatttctgca taataatact
    25501 atcaacacga tggctggtaa ataatatttc catatcattt ctctcgtaaa caatgcaaat
    25561 ggcgacgaat gtatattgcg taatgtaagg acatgccaat ctaaggaatc catactggaa
    25621 ccatctctac cggttttgaa tatggtagca cgagccaata tcaacaaaac cacaaatact
    25681 attgacatta gagaaatggc ttgccgtctt tccatatagg aaactactaa taacacaagc
    25741 gctgccaata acaagaataa atgagcaaat tgcaggatga atattaacat caatatcaaa
    25801 gaaaatcgaa tagcattatt ccatttttcc gcttttaata tcgatgtcat cacaagcaaa
    25861 aatcctactg attgattgag ttcatcattg acataaaaaa aggaatcatg aacagtgagt
    25921 attaatgaaa acaaaaaaat atacgctata tatttttgtt gacacatgta tcgaataaat
    25981 aaaaacagta ataaaaacag tagtaccgga cttaatgaat atgcatacat taccatattt
    26041 aatggcaatt gacatttcac cgccaagaca ggtagccatt gagtaagcac tgcactatat
    26101 cgattgtatt gtatgcagaa gtctttatgt tgaatgattt cgaataggta atagctattg
    26161 tcagcatagg tgcttctttc tacataaaaa tgcagacaca aaaacatata ggctatccaa
    26221 aacaggctaa catacagtgc ttcagaggaa actatttttt ttatcattgc cttataaaga
    26281 tattagattt tactcttttc caatgcttgt tgtatatctg ctttaatatc gtctatgtgt
    26341 tctaggccaa cagacacacg tacggttcct ccaaaaatgc cagctgcttc acgcactttt
    26401 tcgtctattt tgctgtgcgt cgttgatgct ggatgtgttg caattgtgcg gcaatcgccg
    26461 agatttgccg tatgagaaaa catttctaaa ctatctaaaa atttgcgccc tctatcgata
    26521 ccgcctttta cttcgaaggt aacaacgcca ccaccttgcg acatttggct gatggctaat
    26581 tcatgttgtg gatgcgatgg taaaaatgga taaattgttt tttgaatctc ttcatgttgt
    26641 tccaaccatt gtgccaatgc caatgcgttt tcgcaatgcc tatccatgcg cacatgtagt
    26701 gtttccaagc ttttcgacaa tatccatgca ttaaacggcg acatagctgg tccggtttgt
    26761 cggcaaaatg ctcgcacttc ttttatcaat tctttattgc ctaatacaat tccgccacat
    26821 actctgccct gtccatcgat aaacttcgtg gcagaatggg taataatgtc tgcgccatat
    26881 tttgctggtg tttggaggta aggagttgca aaacaattat ctacattcac caagatatta
    26941 tgctttttcc caagtgcaca caagtgtgct aaatcgatta acactaagcc cggatttgat
    27001 ggtgtttcgg caaataacat ttttgtgtta ggtcgtattt ggctttccca tgtttctggt
    27061 tggctgatgt caaaaaagga atattctata ttccattttg gcaaataatc tttgataatt
    27121 cttgttgatg aggcgaataa tgctgatgat gccaagatat ggtctccagc atttaaaaat
    27181 ggcatcatcg atgcaaacac tgctgccatg cctgttgctg taccgaagcc gtcttctgtg
    27241 ccttcgagca cacacatttt ttcgacaaac tcattgacat ttggattgga aaaacgagaa
    27301 taaatattgt ccacatattc atcagcaaat aaagctcgag cttgttctgc atcgtcaaac
    27361 gtaaacgatg aggttagata caaaggcaca ctatgttctc tgaatgcgcg ttcctgttgc
    27421 gtgcgtatcg cttgcgtagc aaagttggta tattttttca tgtgataaga tttgagattt
    27481 gagatttgag atttgagatt ttttctcttg tctcatatct cacatctaat ggtctaattt
    27541 tttatttcgt agctgtgtgt aattgttgcg gttgaattga gcatgtgtgt tacgctacaa
    27601 tatttatcca tagaaagtgc aattgccttt tctacatttt ctttctttag ctttccttcg
    27661 agtgtgtagt ggatatgtat ttttgtaaat accatcggat gttctgttgc tctttctgca
    27721 tcaacactta ccttatagga ttttacctct tcgcgcattt ttttgagcat agaaagcata
    27781 tccatagaag tacaaccacc aagcgccatc agcactaact gcataggtcg tgcgccaagc
    27841 tcttcaccac caatttctgg cgagccatcc atgtgcacaa taacgccttg ttcatttttg
    27901 gctgtcaagt gcactttctc atttactctt tcaatttcta ccttcatgat ataaagttag
    27961 agcttattcc atattagtca ttaggttttt gtgttttttt agtgttaaag ctaaaaacaa
    28021 aaaagtctgg caacgtaata cacgatgcca gactttataa ctgtatttta aaaattaaat
    28081 tttaaacaat gcgtacacga tagcattggt gtggaagatt tttttattgt ctaatggaac
    28141 aataaatttt gaatttgtaa aatcgataaa gccttgttgc actttagcgc caacaccaaa
    28201 accagattta gacacaaact caaaacctgc tgttacacca gcatcatacg ctctaaacgg
    28261 acgattgcca ttaatatctt tgtcattgtg tttgcctaag aaattatttt ctttatcgtc
    28321 aatatctgtt aatgcatcat tcttgaaatc aacttttgtt ctttgttgta cgtttacggc
    28381 atacgatgca aaaccaccta cattaaagtt gaataccttt ttcttgccta cgtaaaactt
    28441 catcgttact ggcaattcta ttgaatgaaa atttaaacga tagaatgatc ttctgtttaa
    28501 tgcatcatca ataaggcgtt gttgcgttcc tctgttggca tagtttacgc ctatttccaa
    28561 gccaaatact tttggtgctc tgtactcata attgacacca aaaacgccac ctggtttaat
    28621 ggtatttttt gaattatcat aagtagaatt tttttgcaat cccatcgtgt tcatagaaaa
    28681 acctgcgttg aagccaacta agtgtcgttt ttctgaaatg tctctcgcca tcgatgtggc
    28741 gatgcttaaa gcactaattg taaaaagaaa gattgatttt ttcatattgt tactgttgtt
    28801 tgattgtcca cgtttaaagg tcaaaaataa taccaaatat aatagtgaca cgcctttttt
    28861 tcaaaaatac ttagtatttg gtagttcgta ctgagtattc gaaatgatga aattcctttt
    28921 tcttatttag tagtaatgac gacacgctct cctttggaat gcgatttgaa ttcgggcgca
    28981 tacatgcttt caatttgtgt aatgccattg ctaaagtcgc ctgctaaagt tgcgcgcaat
    29041 gcatattcaa acacataaat gcctttattc atccaatcca taaagaagtg ggtggccaca
    29101 tcttttgtcg tttcgtaata acctaatcca tcttgatatt tgtactgcga caacacattt
    29161 atcggctcga aacctgcagc acgcatgtct tttaagtgta catattctaa gtttctatcc
    29221 gtgcggattt cgatacgaac ttttatcaca tcgcccactt gcaagggtgt tttatcgcta
    29281 atgcttatta gctgtttccc ttcattggta tttatttctt tgtataattt tttactaagt
    29341 tgcaaggccg ttgttgcgcc agaaatttta tctaaatctt caaaatattg ccaatatatg
    29401 gcaccaaaag ctggaccttt atctgatttc ttcaattcaa tttttgccat ttccggtttg
    29461 atatcatcgc cagaccaatt tactttgtaa taagctgttc ccggttctac gattgcattt
    29521 aattgagtcg gattgattgt tgttttattc attttcacgt ccaccaattt atccgatttt
    29581 atccaatcct taccttgcaa caataatgca tagcatgctt ctgctgttgc tttggttgac
    29641 ttccatgatg tagtttgctt ttgttttagc aaccacactt tcatttcatc taccgagttg
    29701 ttgtctttag ccacttcgtg gaatgcttca atcagcaaag cctgtgtttc tacaggtgct
    29761 tgataccaat accagcctgc gttttcattg gctttccaat acatgcctaa ttcttcgcta
    29821 ttaatggcat tttgttttaa agacacgact attttatctg cgctggcttt ttctttattg
    29881 cgatgtaagg ttaatgctat catgcctttg gcatataaac tttgatcgag ccaaaacatt
    29941 ttttcttggt tgataaaata attatacgct tcttcatctt ttttgtctat tgaataatta
    30001 aagaatgaac gtgcatacaa ggcatgaata ttatcataat acagatgatt ttttttcaaa
    30061 taagctacgt catatttttt gatattgact aaatcttctg tcaggcgagc atctaaatat
    30121 gcgattgctt tttgtaacac ttcatcgatt ttagatttat ttttgatatc gattacattc
    30181 aattttgcca aatgtccaaa accagcaacg atgtgttgcg tgatgtaacg attgtcaggc
    30241 atgcctggaa accaagtgaa tccaccattt ggcgtttgca tttgaataag cttgtcgaat
    30301 gctcgttgtt gctcatcagc catttttgac aaatcaaata aaagtgcaat acgctttttt
    30361 tgttctgttt cattttgtgc ctcgcgcacc caaggtgttt cttctaacaa taccgctttc
    30421 aactcttggt ttttttctaa gttagacaat agtgcatctg tgtttttcca tttatcaaaa
    30481 atgcttttga tttttggatt ttctgtggca atatagctgg ctaacgaatt ggcataatag
    30541 cgagaaaata attgctctgc gcattcatat ggatattcca tcatatacgg taatgcttgc
    30601 acagcatacc acaccggctg cgacgacatt tctaatgtaa gcttataatt ttttaaagtc
    30661 ttggatttgt tgtttaataa cttatcgaag gtgaatgatt ttttgctttc gccgcgcacc
    30721 cacaaaggca tgatttctgt caccatcatt ctattaggta atacaattaa agaagcctgt
    30781 tcgccatcgc taaaattatc tgcttttgct actattttat aagtgattgc atctatattg
    30841 cttggcacat gcagtgtcca gcttacggca atattttttc ctttttctac ggtaaaatct
    30901 tttgctgtaa tggctgaatt tgcgccagca tgtaactgta taaattgtgt ggtaattggc
    30961 tgcatggtgg ctgcatcata gatttcaata tcggcaacgc cagacaaatc tctatcggac
    31021 agattactga tttttgtaga aaattctaat ttatcacctt cccgcaaaaa gcgtggcgca
    31081 ttaggtgtta gcatcaattc tttttgcgtc acaatggatt ttgtaaaata ctgtgatttc
    31141 aaatctttgg tgtgtgccaa gcctaagaaa ttccatctcg tgagtgcttc tggcatttgg
    31201 aaattcagca aaatattgcc atcagcatct gtttgtaatt gaggcaagaa gaatgctgtt
    31261 tcattgaggt tggttcttgg tgttacctca ttttttacag ctggttttgt tggcgcttgt
    31321 gttggcgcag aatctgcttg cacacttgga cttggaggtg ctatttcatt agcaaatgaa
    31381 tcggattcat tttcggcgtc tttaggtgcc gagcctttgc caagcattgc acctgccgat
    31441 tctgatttca ttgacattgc attataagcc aatcctgatt tagataaaaa tctagaataa
    31501 taagagccga tggacaaacc aaacaaattc aaatcatcat aaccatgata tgcatactct
    31561 tttgttggat tccatttttt agaagtgtaa acaaggctgc tcttgctatt aaaagcatca
    31621 tttccaaaac caccaagata gccattaaaa taggattgat tcaaatagaa accaaagctg
    31681 tgcggcagaa atgcatctaa ggaagcatca tacatcgtcg ctaataattc ggctgttact
    31741 ttttctttat tgctacctgt gatttttaat ttccattgtt catttgcgcc tggcaatagc
    31801 ttatctctaa aagtaagcca ttctattttc aggcttttgt cttcatatgg tactgtaata
    31861 aatgtagtct tgctatatag tctattttta ttgactaaca cataattcac ttcaatgcca
    31921 ccgatatagt tttcttttat tgcaatagga attgttttta tttcattgtt tagcgtaatc
    31981 atcttcttat caatcaattt tcctttaaaa gcaatttcta gtattgcttt cgtattagac
    32041 atcgatgagc ctatttgaat atttacggtt tcgttaggtt tcgctgtgtt tttgtcaata
    32101 tggaaataag caaatgttgc tggttttggt gtttggtcat ttgatgatgt tgcaataaat
    32161 gattttttaa atccgatcag attgtcgttt ttatctttac aagaaaactc tatgatataa
    32221 gcaccacttt ttagtgaatt tgatggaagc actatttctt tttgtgtatc tgtattaaaa
    32281 gcatagctat tgagtagcat acctttttgc caattattca tcaaatcttc atttgcatat
    32341 acatcatacg gaaaaagatt atcgtgttct tcttttgtaa gagcgaactt atcaggagca
    32401 ttccatagtc tagatttttt tggattttgt ggagctatta attcataaat ttttatactc
    32461 actgctgttc cttcgtgttg tccatttagg ttgtttgtgg tgatgcttat tttattttct
    32521 attgatgtgt tgatattatc gctcacattc aaattggctt caattgctaa atagccaaca
    32581 cgtactgttg tgcttgctgt atgtgtttcg ccgttgatat ctacgacatc tgcgctaatc
    32641 tcataattaa aataaggttt aaattctttt gaaatcgaca aatccggaat ggctttaaag
    32701 tcgataatga aatttccatt gtcatctgtt tttgtaatgc cattcgtgat aattttttcg
    32761 ttgctggcag gaaaccaagg actgcgccag ccataccagc accacgatgg aaattgtgcc
    32821 acgcgacgca ctgtatattt tacttcggca ttgtcgatat ttgcaccagc ataagacact
    32881 gctttaccgc taatgtttat ttggtcttgt aagttgtacg atttttttac atcatcaaat
    32941 aatacttcaa attttgggcg tttatattct tcaatttgca cattggtaga gccataattt
    33001 gtttccaaag tcatattgcc taacaagcct gtcgttggtg cggtgaatgt gccagtaaat
    33061 gagccgaatt cattggtggt aaactgttgt gtgctaatat cttggtaatt gacatcttta
    33121 aatgtgatgc taactttttc attaggtaac aatgtgtgct gctccttgtt ttgctgagtg
    33181 gcaatacctt taaaatatat gatttgacca ggtcggtaga tgcttctatc tgtaaataat
    33241 tgaatatttt tttgcttgtt tagattctcc tctcttgcat aaaaatattt gtagaacgat
    33301 tgttcactcg gcaggaaatc atctttataa aatatttcga acaagaaatt ctcgttgtag
    33361 taatacttgt ttgctgttga tttatttact tttttcgtaa ctaaaccatt agcatcagca
    33421 gtagccgtgc caatgtctgt acgaacgtat tttcgcttat catagtcata cgtatttcta
    33481 taaaatttca ccattgcatt ttgcaaagga tgtccattgt ctctatctaa aacgtacagt
    33541 tcaaattctt ctttattgtt attgtttcga gttgtatagc taatttgaga aacaaaaatt
    33601 gtgttcattg cgaccgcatt acttccattc gcaagtgtaa ataatttatc cgttgatgct
    33661 aacaatacgt agcaaccttg tttaatgccg tcaactttta tttctgtgga atgttctaaa
    33721 aaatctgttg ccactggcgt tgtaatgctc catgacttta ctggttttat agcattgatt
    33781 cgctttaaca catcatcttg cgtttcttta tttttgattt ctaaaatgtc ttctttttta
    33841 gcatatggca atggaaccag cttaaaatat actgtcgaaa tattacgata attgagtagc
    33901 gccttaaatg gtgttttggg cacaactgct tcttccgttg tgatggaaat gtttttatga
    33961 aaaaaagttt cgcgtaatgc agcacaattt tttgcgcctt ctgtttccgg atattttttg
    34021 atggtttcat cacaaatcgt gagtgcctct ttaatactat ttctatcggg cgctgcattg
    34081 tttaaaatct tgttttctga cgtggcattt gcagcaatat attttgctat ctcatagttg
    34141 attgtcgcgc caatactaca tttctcatat tctttttgta aacgcaataa agcttcatag
    34201 tacaagcttt cattttcttc tttattgtta taatgttctt ttacatattg tattcgtagc
    34261 aagtctgcgt cagctaatgc tactagatta tttttatctg ataagcgaaa gcgcaataaa
    34321 tcttggtata tttttaatgc ttgataatcc tgcgcgtttt tatctgcatc attaagctga
    34381 attgtaataa aatcatttac cgtagcaaaa tattttttat catcaattgc aaatgcattg
    34441 cttggctcag cgatattgct tttatcgtca ttaaagtaag aaatagcacg atttgccaat
    34501 aaatcataca atgttgggcg aaaaatttgt gagcctttta cctgttcgat tataggtgta
    34561 tatgcatcta atttaatttg ctgcaagatt gttttttcgc tgagcgaggc aatataatat
    34621 tggttaattt ctttaaacaa tgttttcaaa tcccatgttc taaaatcatc tgatggtttt
    34681 atgtctgttg ctgttcgatt cgaaaatttc catgcatttc tgttataata ttgaaaatac
    34741 aattctgcca aaatgctttg taatatggac ttgtcggcac ctttagccat atttatttct
    34801 tgctttaaac ccactactat ttttaactcc gaattttctt ccaatacttg tatatactta
    34861 tatttataga gcaatgcttt tactgtttgt actgtattgt tttcatttct tgcgttagca
    34921 attaattttt ctacagcaga aagagcgtct tttgtttttc cttcattttc aaattttgct
    34981 acagacttcc aaagtgcatc atagtttgct tgtgccataa tttgctgtgt ggttaagaag
    35041 agcaataata ttaaaaagct cattttatgg taagttgatt tcatcgttta tcttttttca
    35101 cagtttgcaa tatattgtta ctgatgtgtg tgttttaaaa aattatttat tcgcaatgct
    35161 atattttctc tgatattcat ataaaataaa gaatagtcaa aatgatgata atttttcatc
    35221 accatagaca caaaactttt atctgctggt ttttgaattt ctacaatacc gcgtgtgttt
    35281 tttgcaccaa aataatcatg tattatatga ttgtttttcg gcgacaagct gcctttattt
    35341 gcttgacctg agacaaaagt attatcagta gtccagctta atggatttac agaaacggca
    35401 gtagtatatt caggtacaat tgattctatt tttgaatttt ggccaaaagt agaaaaggaa
    35461 acataagtac catagctatc tggtgtatcg gaaactttca aatactgaaa catattttca
    35521 tgcgttggaa atccgatgat atatgcttct actaattgtt tttgcaaagc tttgccatcg
    35581 aaaaactcgc gcaataagcg ttgtgcatgt ttactacctt gcgaatgacc tgcgattatg
    35641 attggtctgc cattattgta atgaagcaaa taatattcga atgcttgttt cacatcggca
    35701 taggcagtgt cgaatgccag ttgtgcacgt tctttatttg atgtaaaaaa attatttaat
    35761 gcagcttgtc gatagcgtgg tgcatacact ttgcaagatt cattaaacac gcttgcttga
    35821 tattttattg aaaatttatc ggtttgcttg ttcaccattt tatcgtcgat gcttgcattg
    35881 cctttgattg cggtgaaata gcttgttgga tgaataaaaa atacatcagc gcgtgcgctg
    35941 tcttgtttgt ttgccaatcc tgtaggcgtc caatctgcat tgtcttgcat tgtaggcaat
    36001 gctgcccaat tttcttgctt tgcataatct gttgcataca ataaattata cattgagaat
    36061 agtaggagac aataaaacaa tttcattttc attttttaat cgcttcaaaa taattagcca
    36121 ttgaattcaa ggatattttc ataacgcaaa ttacatgcct tttgtataaa tgcaatataa
    36181 ttttacaatt attatgatac ttctatcgct taggaattgc cgccgagcct aaatcctaca
    36241 gtctttcgca gtgcatttgg ctgataaacg aactccgtaa catcttctat tataatcgta
    36301 tttatatctt gcgatgtacg ctgcgctgct ggaatttttg ccatgcgcaa gttttgattt
    36361 ttggttgcct cacctattgt ttgtcgcaca gtacgtgcgt tgccaaaaaa tttatctctg
    36421 tgtttataca aatctgtgaa gtaagtattc aagtgtaatt tggcatcagg tgtcaattgc
    36481 aaatcatctt ttttcagaat ggtttctgca atctgcatca tttcgtcagg cgtataatct
    36541 tcgaaatgca ttttcttatc aaagcgtgaa tttaaaccag gattcatgct caaaaaatcg
    36601 ttcatgtttt ctggataacc agccacaatt agcaaaaatt ttcctctgtt atcttccatt
    36661 cgtttcaaga tagtgctcac ggcttcgcta ccaaaatcgt gttgagcgcc tctgccatct
    36721 gttaatgcat aagcttcatc aataaacaaa acgccgccca tggcttcatc aatttttgct
    36781 gctgttttga tagcagtttg tcctacaaaa ccggccacca atccttctcg atctgtttct
    36841 acagtatggc cgcgttctaa gatgccaagt gccttatata tcttgcctaa gatacgtgct
    36901 accgtggttt ttcctgtgcc aggattgcca gtaaaaattg tatgcagtga gaacttgttt
    36961 aatacatctt ttccgatatc attataatag cgaataagat gcaccagctc gtcaatttct
    37021 gttttcaccg cttgcagacc gatcatttca tgcaattcgc ttaaagcttc gctgagctct
    37081 gcttcattta tttctaattt taatgaggtg cgttttctat cttcaaatac ctttacaata
    37141 tcttcttttg ctatggtgga caattgcgct ttagataatg atttttggtc aggcgcattc
    37201 atcaagcgaa gtgccattag catttttgct ttattgataa caccttcggc aaatcgtgcg
    37261 tttccaaagt ttttatctct atttctaaat gcgtctgtta attctaattt caatgttttc
    37321 ttagcttctt cgtcgatgtg taatttcagt ttttctgcga tgctcaatgc aatatcatac
    37381 agctcttcag gcaagtaatc atcaaaggta aaatattgtg aaaagcgtga ttttaatcct
    37441 ggatttgatt ctataaaagc atacatttct ttaggataac ctgccacaaa aattgcgatg
    37501 tcgccaggtc catcgctcat ttctttcagc aatatttcga tgacttcttt gccataatct
    37561 ctgctgtctt cgttggtgcg tgcgagcgaa tatgcttcat cgatgaacaa aataccgcca
    37621 cgagcttcat cgatttgttt ttgtgttttc ggtgctgttt gtccaatgta ttcgcctacc
    37681 aaatcaactc tatctacttc gagtacatgt ccttttgaga gtaggccaag tttgtgatat
    37741 attttgccga gcaagcgcat gactgttgtt ttgccagtac ctggattgcc aatcgcaaca
    37801 ctatgcagtg aaatcgatga gttgtcctct acgccttgtt ctttgcgaag tttattaaaa
    37861 ttgataagtg ttatttgatc gcgtatgtgt ttttttacgt gttgtaaacc gattaattta
    37921 tctaattctg ctaaaagttc gtctaatgat ttatcgtcca atactgttgc cgtctcattt
    37981 tcgatagctg cttttgctat gccaggaaca agcgtgccat tgaataattc tagttcacct
    38041 tctacaaaaa catcgccaca attaaatgct gcggctgcca ctaaagtatc cataaacaca
    38101 agctctatcg tgtaacggtc gtctttccaa gagccgccat catcagtgcc ccagccaata
    38161 tcaaacgtat aagaccaatc gaatttatta gcttcaattt ttcctatgcg tgatgttacc
    38221 gctttgtgtt ggccagcatc gtcataataa ttaaggaata attcataatt ccaatctttt
    38281 tttgttttgt tggtcaattt tatttctgcc cacagatact ttgtttcttt tttagagatt
    38341 tgagagaagt attttctatc atttgctgcc tgtctccagc cttctgcatc gccgttgtat
    38401 agctttatgt attctacgga gaagtatgga tttttttcgg tgcttacttg accgacatca
    38461 ttgacataaa aaagcttagc ttcgacaaaa acatcatcga tgtatgcttc ccaagtgtag
    38521 tttcctttta tccaaaattt gccagcatct tgattgcccc aaccatcgcg aacatacacg
    38581 atattttcta atttagatac ctctacctct ttatctaggc tgcataattc ttctcgtttg
    38641 ttattggtga tatcaaatgc tttaagcgtt attttacact tccaatcctc ttcatcgaat
    38701 aacttattat aaaacgaaaa ttcggcgcgt aagtaagttg tttctgctct gtcaaaaact
    38761 gttctgtatt ttttggcagc attagccatc cattcatcag aactgtaaac gagcaagctt
    38821 ttaaatttgt attttacaaa gtttttatcg tcgctttttt tatcatcttt atcgttattg
    38881 ccaaaaaaca taggaataaa gctatgaaaa tatttgtaat ttatagcttt attgattgaa
    38941 tgagatttgc tgaattcaag taatacaagc aatatttttg tgtttttttg tattacattt
    39001 aattataaat cgtaatacat agcattgttt gtatctttgc cgtatgaaaa aagagcaacg
    39061 cacggaaatt tcatcgcttg gtgaatttgg tttaattgac agattaacaa aagatatttc
    39121 attaaaaaat acttcttcta taaaaggtat tggcgatgat gcagctatta ttcaggcaga
    39181 aaaaaggcaa gtaattacta ctgatatttt aattgaaggc attcattttg atttggtata
    39241 tacaccattg aaacacttag gttacaaaag cgttattgta aacctatctg atgtatatgc
    39301 gatgaatgca acgccaaaac aaattttagt ttccattgct atttccaatc gtttttctgt
    39361 agaagctatt gaagaaattt atgaaggcat tgaactggct tgcaacaaat acaatgtgga
    39421 tttagttggt ggcgacacca cggcttcgcc aaaaggtttg gtgataaatg tggtagcgat
    39481 aggcgaacag gaaaaagaaa aaatagtaac acgcaacaca gcaaaagaag gtgatttatt
    39541 gtgcgtgagt ggcgacttag gtgctgcgta tatgggtttg actattttgc aacgcgagaa
    39601 acaagtgttt ttaaatgcac ctgatgcaaa cattgaacta gctggcgaca attatattgt
    39661 aggcagacaa ttgaaaccag aagcgcgcaa agatattgta gatttttttg ctgaacaaca
    39721 gattgttcct acttcgatga ttgatgtttc tgatggtttg agttcagaaa ttttacacat
    39781 ttgcaaacaa agtaatgttg gctgccaact caatgaagag gcagtgccta ttgccagcga
    39841 aacatataat aaagccttgg agtttggtat cgaccctatc aattgtgctt taagtggcgg
    39901 cgaagattat gaattattat ttactatttt gccgagtgac agaagcaaag taaaacaaca
    39961 tccagacatc agcattattg gtgaaatttt agatgaaaac ttaggtgtaa aattatttac
    40021 aaaaggtggc aattatcacg atattatttc cttaggctgg aatagcttaa aagaaggcga
    40081 ttaacattca tctttagcaa gacttaccca attaggtgca tcgtttatgc tttgtctaag
    40141 caacagattt aattgtgcta catgatgttg tacatgtcgc atattataca ataatacttc
    40201 taacatcgta taatctttat attcatttat ccaacgtgcg ctgagttgtt ctgtcgtcaa
    40261 tccagcaatc agcgtttttg ccttatttct acaatgtgtt aaatacagca ttaatgctgc
    40321 ttttgaataa acgatgtctg gctttttatc gggctcatat tcagacaaag tatatccttg
    40381 aggtggcgca aaggaaagtg gttctgttgt cagaaaatag tctgtccaaa agatacaatg
    40441 atatgtcgtg taccaaaaat ctaaatgtgt attccaatgc tcatctggac acatctttat
    40501 tgcattttcg agcatatcga ggctcgctcc aaattgtgtc caaatggatt cgtttatcgt
    40561 tgttgctttt ttcattttat taaaaattat gtgtcctaat tatcggtttg caaatatgcc
    40621 actatagcca gtgattcttt ccgtcaattc attttcaacg tttttttata agttacattt
    40681 gtccgagata agcaaaaaca ctaaatcttt tgtcaacgct aattaagttg tcaattaaca
    40741 ctaaacaact atctaagtcg cggtaacaaa atggtgcatc tgttttaatg ctgtcgtttg
    40801 caatcatttc tgggatgtaa atcttttctc tcaatgcgtc atagtcaaat gttgaagcaa
    40861 attctttggc ttctcctctt gacatcaccc gtcctgttcc gtggcaaaga gagttgtatg
    40921 ttttttcaac ttccttggtt gcggaaatta aaactacatc tccattcata ttagagggaa
    40981 ccactgtctg ttcgcctggg cttactttga cagcaccttt tctaatgatt acaccattgt
    41041 cagtttgttc aaaatggtta tggtttttgt caagaattag atggagttgt ccgaaatatt
    41101 tttctaatat tttgaaaatc gcaaatctgt tgtcttttgc ccattcacaa acatcttgaa
    41161 actttgtgtc aaatttttta gactggctga ttaggtcgtc tacaagtttt gattcattac
    41221 gtgaacccgt atgaataaga aaatatagtt tgtcgtcggt atatgattct aaagcgtcaa
    41281 gaaaatggtt gccagaacct aagtcgccaa gtttaccctt gttagcttga atgtcaaagt
    41341 atattttgtc ccacttttct cttttaaaat ctgctttagt taagtctgct tcgcacagta
    41401 acattccaca accgcagtcg gatatcgcaa acttacgcca gtcctcttgt tccgtataaa
    41461 caacagttcc tgtcggaagt ggagattttc caggacaagc atctggaaag aatataactc
    41521 tctctgcttt caagtcatga ggcaaccaac catatacttt atttaaaggc tctgatgaat
    41581 aatttattac ttccattttt cgtattctta tatcgtccta ccattggcta tatcattcta
    41641 acaattgcct taattcacca aactatcttg atgtataatg acagtgctgt cttttttgat
    41701 gaatttttca gcatcagcta atgctttttt tgtagcagca atagaatctg ctattttctt
    41761 tttaataagt gcgacagaat cttgtatttg cctttcgcga atttcttctt ttgtgagttt
    41821 gtgtgcttgt ttgccattaa aaataaaatt gattaactgc ttatctctgt tgtacacatc
    41881 agaataaaat tttacttcgc cattaaaatc tgcttctgtt gtaaaatatc tgaagtaaac
    41941 tggcacaggc ttttttaaac gaaatgtgcg tgtagcaatt ttgctatctt ttaaagcaat
    42001 taatgaatcg taagtaattt taaaagtatc catcatcatt aaattggcca cgacttccaa
    42061 aggatcttga cagcgcacac agccgtggct tgctgcacgc aaatctgctc caaaggttga
    42121 ttttgttggt gtatcgtgta tatacacgga atatttatta ggaaaaagta ttttaaccac
    42181 acctaatgca ttgcgtggtc caggtggctg aacaacgctt gtccagccat tatttttcgt
    42241 caccacataa cctttaaaac ttttcatctc gtgctttatg atactttgcg gcacgctcca
    42301 agttggccat aaaacaattt gattaatggc actttctaaa attggtgttt catttttgag
    42361 gttggatttt ccaatcacta cttttttctc caatactaaa gtatcatggt caattatttt
    42421 taatcgataa gaaggtaggt ttacccaaac ataacgttca ggtaattcga tgatgtgttc
    42481 cttgcgccat cggtctgcat taattgccaa caattgaaat tttttggaat tatcgcgctc
    42541 taaagctttt attgtatttg cgccaatagt gccatcggca tttaggttgt tttctttttg
    42601 aaatcttttt agggcgacaa taaacgcaga gtcattattt ttcaaagagt ccatcaaata
    42661 atgatgatat accaatgctt tccgcgcatc tgttaatgca cctagtgaat cgagctttgg
    42721 atttctgatg ctaattggtg ttgcagaaat attgtttttt gccacaaaag cgcgcaatgc
    42781 tgacatgtat ctattataca aaggattgtt gggctcgagc gatgataaac ttttgaaaat
    42841 agaatccgta acggctgttt gcaaaagttg gatgtattta tttttgtctt tccaaaaatt
    42901 agacgtgatg ccttgtgttg tgtcagcaaa cataccatga tcgagatgca atgccatttg
    42961 caaaaaggaa cgcgtaaggc cgacttctaa ttgtttggct aaattaaaat caacggaagg
    43021 tgataagctt agcagcgaat catatatttg ttgcaattgc tcatattggt ataaatcttt
    43081 gtttaatgcc aaaaattctg ccgtgaccaa aacgtgtagc atttccttgc cttttggtga
    43141 caaaatatct ttgttcaacc ataaagattt ataattattc agcaaataaa cttcctttac
    43201 aacaagctgc cagttttcat attttgtgct atcatttttt atcgcattta atgtcggaat
    43261 atattcgcgc aatattgttg tgatatcttg ttggaagcta atctcatctt ttggtgcctc
    43321 cacttttttc tcgatatctt ttgcatgatt ggaattgcat ttacaagaag taaacaagca
    43381 ttgcatgcag aaaaagaaaa tggtaaatag taaaaaagta gttgctgaat tcaacttgtg
    43441 ttcgcgcttc atggtcaatg ctattgagtt ataaagtaag agaaaaaaac ggcaacttgt
    43501 atattgttct gctaatttaa cttagccgct aacacgcatt tgcgtttata gattattttc
    43561 tcaccatcag gtttttttct ttcaaatact aaaaaataaa tacctacatc tttcttgcta
    43621 ccattttcat catcgccatt ccaaacaaaa gcaccttctt tcgctattgc ttcatctttt
    43681 accaaatgat tggcgagttg tccagcagca ttatataaat aaacattaac cacgcttcca
    43741 tcttcatcaa atgaataggt aatttttgct tcgtcatcat atccatcgcc atctggtgaa
    43801 aacacttctg gaataatatg tacattgccc aagaggtgat actcattttc tgaattcaaa
    43861 tatgttggtg tagcaaagcc agcagattct gcagcagaat gccaataacg tttgttttgt
    43921 ggcaaatcaa atccgctttt ttccaaagaa acgcctttcg ttgtttttaa taatggaaaa
    43981 tgccaagacg tctgataatg cagcttgtcc agcacttcat cgtttctgtt ttttagcagt
    44041 acaatatctt cttgtgtatc aaaagtcggc aggtttacat ctttcatttt ttccaaaatc
    44101 gctggtggat attgtgattt tatactgatt ttatttcttg taaaaacaat ataaccattt
    44161 gcaaaaatat agccttctgt atttgacaaa tctaaaagtt gctcttcaat ttccgtattc
    44221 ggatttgctt gtgcaaattt gatatcgctt attttaaaaa gttttgaaga attattatac
    44281 aactctacaa actgctcaac accggcatcg gcatggaaca aaatttcatt gataataagt
    44341 tctccatttg taggtatttc tgttatggca atatcaaacg tatcagcaag gtgtatatat
    44401 ttcacacagc cagaaaatcc tgcaacagta aaagtataaa taatattagg ctgcaaggca
    44461 gaggctaaag tcaaagtcac tttcgacgca tacgtattag caagcattat gttctgtatg
    44521 ctaatgccat tgtctatttg ataattcgaa atggactgcg ctgaagtttt gtccattggc
    44581 ttagtgaagt caacttcaat agttgtatta ttgattagtg taaacgtggc gtgcacatat
    44641 tctggtgctt catttctatt catctctcca ggtgttccgc catttacatc aacagaagct
    44701 gaccaaaaca attccttatc gcagtcgcca gtataagcac tcgatagcaa ctctaatgac
    44761 cagccaccat tttgttttat aacgtcgtga tacatcgtat caacataatt aagctggtca
    44821 attaaaatag tgtcatcggc tctcaatgta agcgtaccac taaacgctaa attattccaa
    44881 ttgtctaatg ctatcgtatt tccaaatggc ttgaataaat ttgtatctgc tgctgcacat
    44941 aaaatagcat aagtatttgc tgctatcgtg ccttctaata cgtttgcatt gttaatggtg
    45001 aaatttttta aggaaatatt ttcgttgctc gtatttttta attcaatgta ttgtttgtta
    45061 ggcaagccta atgcagtcga tggttttatc attatttcat tgataagaat gtcattcaaa
    45121 actgcataat aaggtgtgtt aaaattggaa atattcggta ttgacatact atttccagca
    45181 gagtcagcta cattatttgt cgttagtgta taagttgtat tggacaaaaa ccgttgtgca
    45241 aaagtaagat gtactaattt tttatttaat gcatcaatta ctgctgtcat aggattgcca
    45301 agacttgaaa gcgtatagtt gttgattagc tgtgcgcttg ttatgtcaac agtttcatca
    45361 aaataaatat cgacttgtgt gctatttatc aaactgacag tactaacgct tggtggcgtt
    45421 acatctaatg acaataatgg cctttgtatg caatatacat catcaaacca aaaactatct
    45481 ttcgatgcag cagtatatgt acacaacata ccaaaatgag ttgttgatgc ataggtaagt
    45541 tcttgcgtgc tatcaagtgc cacatagcta gattgattgg cttctttcca ataaaaaacc
    45601 cacttgccta aattattttt gaccactttt aaatttccat ctgctccatt ttgaaaattt
    45661 cctgctagca tgctttttat aagcacattg ctattgccat ctttcctgta aaaatctaat
    45721 gcatcagtat ttccattttg tccaatgcgt acaaaataac catcaaaatc attcctaata
    45781 tccatttggt tagacatgag caccacatcg agcttgtcat tatctgatgg ttgaaaacct
    45841 aaacgcagat acaagctcca ttcaacactt ttgcctagcg tatccgcagt catgatggct
    45901 gcattgccag tattattcga tgcttgtaaa tttaaaactt gattgacaac aataaaattt
    45961 gtgcccaatg tatcccaatg tggattaaca ttaaagcctc catctgtaaa atgctcttca
    46021 aactgtgaaa acgcacagag tggtaccaaa attaataata tgatgtgtaa aaatctcgtc
    46081 ataattgagg atacatacaa atatccttaa tcttagttgt tattcctaca caaatggttg
    46141 ttttggtaat attaaattaa aaaaatgagt gccagctatt cgctacattt gtactataat
    46201 agttgttgtt tacattattt tacaactatt tattttatta ttaacaaaca aacacaaaca
    46261 aatgaaagta gcagtagtag gctccacagg tttagtaggt ggagaaatgt tgaaagtttt
    46321 aacagaaaga aactttccgg taacagaatt aattccagta gcatcagagc gctcagctgg
    46381 caaaaagctt gcttttaagg gcaaggaata taccgttgta actatgcaaa ctgctatcga
    46441 catgaaacca gatttagcac tattttctgc tggtggttct acttctttag agtgggcacc
    46501 aaaatttgca gcagcaggta ttaccgtcgt tgataattct tcagcatggc gcatggatcc
    46561 aactaaaaaa ttagtggtgc ctgaaatcaa tgctgatgca ctaacaaaag aagataaaat
    46621 tattgccaat ccaaactgct ctaccattca aatggtagta gctttaaatc cattgcataa
    46681 aaaatacaag ataaaacgtg tagtagtttc tacttaccaa agtgtaacag gaacaggcaa
    46741 gaaagctgtt gaccaattga tgaatgaacg tgcgaatggt agctcagatg atatggcata
    46801 taaatataaa atcgacttaa atgtcatccc acaaatcgac gtttttttgg ataatggtta
    46861 taccaaagag gaaatgaaaa tggtgaaaga aaccaacaaa attatgcgcg atgaggatat
    46921 caaagttacg gcgacaacta ttcgtattcc agtaatgggt ggccatagcg aaagcgtaaa
    46981 catcgaattt gaaaacgaat tcgaattagc agatattttt gatgaattaa gcaaagcgga
    47041 aggtgttgtt gtagtcgatg atccgagcaa tcaaaaatat ccaatgccaa tggatgcaca
    47101 tggtaaagat gaggtttttg taggcagaat acgtcgcgat gaaacacaag caaaaacatt
    47161 aaacatgtgg attgtggctg acaacttacg caaaggcgct gcaacaaatg ccgtacaaat
    47221 tggtgaatac ctattaaata ataaactaat cggttaattg tttattgtta tatatataaa
    47281 actctgcatc tcgcagagtt tttttatttt tatttttaag gcataactct taaattcaat
    47341 actaaaatag taagctatgc ctataagaat ggtcgatgac gacaatcagc aacaagatta
    47401 tgtacctcgc aatgatggtg gcggtggaca aagaggtggt ggcggtggct tgcctggtgg
    47461 cggttgctta actgcattct tacctatcat actcaaactt attttcaaaa aaccattaat
    47521 cggaattccc attttattaa ttggcggatt tatactattt aagtttggtg gtagtggctt
    47581 gcctacaaat acaaacaatt ctaatgctac agcattggca actggcgccg aattgaaaga
    47641 agaagtatat gacaaagcgg aagttgcggc atcactttct gaatatgaaa aaaatccatt
    47701 gccagaatat gtaagtctat taaaatttgc acccgcacgt atggatcaag gagagcaggg
    47761 ctcttgcgtt ggctggggca gttcatatgc agcgcgcacc attttggagg cttcttccac
    47821 agggcaaaat cctgatgaaa ttgcgatgag tccttccttt gtttataatc aaatcggatt
    47881 ggccaactgc caaggtgctt atattcttaa tgcatgtaag gtaatgtcag aagccggcga
    47941 tattccaaaa aaatattttg aatatacaga ccaaagttgc actcgcgagc ctaatcaaca
    48001 gatacttcaa gcagcacaac aatttaaaat cagaggttat gaacgcttgt ctgtcgatgg
    48061 tgatgattac acaacggact tttttgccat taaacaatat ttagccaaag gcgctcctgt
    48121 catttgtggc atgatggtcg gcggctcctt tatgcaagag atgcaaggtc agaagctatg
    48181 gaatcctaca caatatgact atgatatgga aggatttggt ggacattgca tgtgtattat
    48241 cggctatgac gatagacttg acggtggcgc attccaaatt atgaatagct ggggccctga
    48301 ttggggtgaa aatggtattg gctggatacg ttataaagat tttatgcatt ttaataaaga
    48361 ggcttacgct ttacaaccat taccaaaacg tggcgaaatg gctgctcgta aatttgaatg
    48421 caccattggt ttagtaaaca acgacacgaa acaatatatc aacttaacaa attcgggaaa
    48481 tattttctca acaacgacgc caattgctaa aggaacaaaa tttaaaattg aagttcaaaa
    48541 ttctatagaa tgctacatct atatttttgg acaagagact gatggaagta gttatgtgtt
    48601 atttccatac acacctaaac acactgcatt ttgtggcatt acaggctatc gcctatttcc
    48661 aaaaaaccaa tcactacaag cggataacat cggcgaaaaa gacaaaatgg caattgtcgt
    48721 tactaaagag cctatagatt ataaccaaat aaacacagcc ataaacaagg cacaaggtga
    48781 ttacaaaaca aaagtcgaga atgcgttaaa tgcaatggca ttaagccatg tagaatatag
    48841 cgccaacaaa ggcaggattg catttacggc agaaagtgaa aacaaaaatg cagtagtgag
    48901 tgttattgaa attgataaaa aataaaagct tatgtcattt ctaatttacg gagcagctgg
    48961 ttatacagga aaaattacag tggaaagtgc tgttaaacaa ggcttgaaac caactatcgc
    49021 aggaaggact gaaagcaaaa taaaaccgat tgccgatgca ttcggattgc cctatttaat
    49081 ctttggatta gatgatatcg acaacataac atctcatctc aagaattttt ctttagtatt
    49141 aaactgtgca ggtccattta gtagtacaat taaaccaatg cttaaaacct gcttacaaag
    49201 tggcacacat tatttagata tcaccggtga aattgaagta tttgagtatg caaaagcatt
    49261 tggaaaaaaa attatcgaaa gaaacataat tgtgatgtgt ggcgttggct tcgatgttgt
    49321 tcctacagat tgcatggcaa agcatttgca tgaaaaaatg ccagatgcaa cacatttaga
    49381 attggcttgg actggttagg cgccaatatt tcacatggca caatgactac aatggtagaa
    49441 agcttgggaa aatctggtgc tattcgaaaa gatggcgtga ttgcgctaac tccaattggt
    49501 catattggaa aagaaattga ctttggcacc aagaaaatat tttgtatgac cataccatgg
    49561 ggcgatgttt ctactgcaca ttttacaacc aaaattccaa atattatggt gtttaccgct
    49621 gtgcctaaaa atgcctatta tgccatgaaa atgcaatcct tgtttaatcc aattatgcga
    49681 acttcatttg ttagaaataa gctacaagca tacgttgata aaaatatttc aggccccgat
    49741 ttagaccgta gagagaaagg aatttcctta atatatggaa aaataagcaa tgctaaagga
    49801 gagacaatag aggccaggct gcaaacacct gaagcctata agctcaccgc agaaatggca
    49861 ttaattatta cacaaaaaat actaagcgat aaatcattac cagctggtta tcacactcct
    49921 gcagagttat ttggcgccaa tctaatattg gaaatgccag gtagcaaata tgtataaacg
    49981 ataaaaagcc gcattgtgcg gctttttatt ttctatctaa gatttattat tgcaaaataa
    50041 tatcaaaatc aactaattca ataaacactt gaacgcgctc ttcaatttct tcttcactta
    50101 cttcttttaa tcgctcaggt ccaaattttt ctacacaaaa gcttgccatc gccgagccat
    50161 aaattactgc acgcttcata ttgtcaaaag ataaatcttt cgtttttgct aaaaagccca
    50221 taaatccacc ggcaaatgta tcgccagcgc ctgttgggtc aaaaacatct tctaatggaa
    50281 gtgctggtgc aaaaaatact ctgtttccat ggaacaacaa tgcaccatgt tcgccttttt
    50341 taataattaa aaacttcgga cccatttcac gaattttatt agctgcttta actaacgaat
    50401 attcattgct taactggcgt gcttcttctt catttacgat caataaatcc accattttca
    50461 acacctcttt taatggctcg agtgcaattt ccatccaaaa attcatcgtg tccatggcaa
    50521 tcaactttgg acgcacttcc atttgtgata ttacttttgc ttgtaaagct ggatctatat
    50581 tgcctaatat taaaaactcg gcatttttat atttagctgg taaaattgga tcaaatgtgg
    50641 ctaaaacatt caaatctgtt gccaaagtat cccgacgatt taagtccata tggtatttac
    50701 cactccaaaa aaaggatttc tctccttctt ttacttgcac accttctatg tctgtaccaa
    50761 ttgattttaa atacactaat tcattttggt caaaatcatc gccaattacg ctaactaatt
    50821 gcatatcatt ggtaaaatgg cctgcagact ggcaaatata attacccgat ccgcctgtta
    50881 tcttgtctgt tttgccaaat ggtgtttcga tagcatcaaa tgctacagtt cctactacta
    50941 aaagactcat gaattttttt tttacaaaga ttgcatattt atttttattg tcctacattt
    51001 gcactccgaa ataatgtgtt tgcatcaatt ttgataagaa tacatattct tccttagctc
    51061 agttggttag agcatctgac tgttaatcag agggtcgctg gttcaagtcc agcagggaga
    51121 gccaccaaaa aaaagcactt agattaaaaa ctaagtgctt ttttattttt ttgcaagtat
    51181 tgtttcctaa tttggcacaa gtagcctgta cgcagaccaa cgcctctttc ttgcaccagc
    51241 tttgttaggg ctagctaggt cttactcatt cattatttaa tagaacctgg aatttgtcaa
    51301 caaacagtcc aacgtgaacg ccgtccatta atgcatggtg cccatgaatg gaaatcgcca
    51361 tttctctttt tccattaatt tcggtcattt tgccaaagga aattttagtg caactgtcag
    51421 ggtgcgaaaa gcttcttgcg tgcgaaatac ttgtaaaatc caaccatgga atggccgaaa
    51481 aatgaatgac gttttcactt gatacggctg gcaataaact gcttgtgttt tttacatttt
    51541 caatttcctc taatgctttt tcataaaaat atttttcatc ttcaaaatag tccatataag
    51601 caaagccaaa tgtaccatta ggtctattga tggttggcga agcatttact ttttcaaatt
    51661 caaatacttg attttcgata attctatatc tgaaattttc cacatcattt gctgctttca
    51721 aagccctata taagtaatac aaaaaaaaag aaacgccttt ttcttttgct ttcttgtagg
    51781 cagatgtgca atctactttt atcgttacgc caaaaaatgg ctcctcaaat tttgagaaga
    51841 agaggaaatg ctcctttctg ttccaattgt caatatctat tcgttttttc attctatttt
    51901 agataattca gtatttcgtt aattgatttc agttgaataa aattctcgtg tgttaagttg
    51961 gtttcttgtt gctcgtgtgt ccaagttaca tgataaggaa tatgtgctgc ctttccacct
    52021 aattctaaaa caggaataac atcggatttg atggaattcc ctaacattaa aaaattttca
    52081 ggttggcaat ccaaatgttt gaatagtttt tggtaatcac tttctttctt ctcgctcata
    52141 atttctatgt gatgaaaata gtgttccaat cctgaatttt tcaactttct ttcttggtct
    52201 aacaaatctc cttttgttgc aacgaccaat ctgtaatcac catttaaggc ttctaatgtt
    52261 tcggaaacac cttgcagcaa ttcaatcggt ttttgtagca aatcgtgtcc aatttcaata
    52321 attttatcaa tgacttttaa ggaaactgtt tggttggaaa ccctgtttgc ggtttctatc
    52381 atgcataata caaatccttt aattccataa ccgtaaagag ctaaattctt catctcggtt
    52441 ttgaaaagtt cttgtgaaat ggtatgctgt ggaagatagt tttccagtaa aatacaaaat
    52501 tgtttttcgg cttcttggaa ataaggttcg tttacccaca aagtgtcatc ggcatcaaat
    52561 gcaattactg taatatctct attcatcttt taaatttatt atatttacgt attgcaaatt
    52621 tcgataaatg taaatgtaat aaaaaggaca tttgtcccgg tgttttatgt taaaaacaaa
    52681 cgaatctttc ctaagctatt tatcagcact ttacgaacgc caaagccgta aagaagatat
    52741 tattctaaaa agatgtggga aaggacaaag aatactatct caaaatgaaa ttgccacaaa
    52801 agtgatgttg attgtggaag gaattagcaa atgttatttt acagaagaca atgacaaaga
    52861 atatattatt gaatttttag gcaaaggtga gattattggt gagcttgaat taattcgtaa
    52921 cattccttgt ttgtgtacaa ttgaagcgat gacagctgtt tcttgttttg ctattgataa
    52981 accatatttt tcatcacttc tccaaacaga cattcattta aatcatttgt tgttagacag
    53041 tttcgccaaa cgaattgtag atacagcaag cagagcagct tatcagcagt tgtacacggt
    53101 cgaacatagt ctgaataagc tgatggaatt acagtctata caaaatgttt ctctttcaaa
    53161 agatgaaatg gcttcctatt taggaattac ggttagaagt ttgaacagag cgatgaaaaa
    53221 cttacgagaa tagtttccat tcttatcctt accattgttg gtttgcaagc aacaaactag
    53281 caagcgaaaa aaataaatat atcactttta atgagttaaa cttgttggta cgtcatgcac
    53341 tttgctcggc tgcataccta taaaggaaag gcgaaattac agcatcaaaa catcgtagtg
    53401 agatatgaaa ttactgcatt aatccttcag gtatttgtgc gcctaattgt tttgctgtcg
    53461 caacatcttg ttgttgtgct gatgtattgc ctaataatct ttgaacgtgt gctcgtccaa
    53521 cataataaat tccattggca ttattcaacg caattgcttt gttaaaatcg agcaatgctt
    53581 ccttgttttt gcctaattgc acgttgcaaa caccacgttc gtagtacata tcagcattgt
    53641 taggttgaag cattatatag gcatctctat ctttcaatgc aaaatcaaag cggttcattt
    53701 gcgcatgcaa aattgctcta ttagaatagc cattcaaatt attaggatat aaggacaagc
    53761 ccttattgaa gtcgagcaat gctgtatcta gtttgcctag cattgcattt actgctcctc
    53821 tattaacata catttcgcca atcaacttat tgtctttatt ttgcgtttgc ggataattta
    53881 ttatagcctg attataggct gttaatgctt tcattaaaat ggattctctt tgcacggcgt
    53941 ttaatgtcgc agaaaaagaa ctatctgtat atgctttggc aagattatta tacgttgtag
    54001 catcagtagc ttgtaacatt aatgcttgct gataattctc aatggccttc tgtctgtttt
    54061 tctctgtgtc tctataatac tctgccgcat ttttccatgc agtcggacta ttcggataat
    54121 atgattttac tttttcccaa agcgttccac cattttgcca caccttttgt tgttgtattg
    54181 tcaaataggc aaatagacat aaataggcaa caatacacaa ctgtattaat cgaattttcg
    54241 taggatattt accgaccaat ttatcatagt aaaaagaagt aataaaaaac aatccgatgt
    54301 atgcaatgta ggtaaacctg tctgccaaaa atccttgacc ggcgcccact atttgcaata
    54361 aaaacatgac attgaaagtg aaaaacaaaa tgccaaacaa caatgcatgt aatttttttc
    54421 tccaagtaat aaatatcgag cctagtaata gtatcggaac gataattaaa catacgtatg
    54481 caattattgg taatgttggc tcgtaaggat acaatggcga catcttatat ggaaatatcc
    54541 atttaatcgt atagatagcg taagcataag cacctacggc aagtttatcg acaaagctat
    54601 atgtaacagt tgtgtccgtt gattgtaaag agttggcttg ttgtaaaaaa taaatattta
    54661 tcaaaccaaa aatcaatgac aatagccacc atggcaattt ttcaaataca gcaattttta
    54721 tgcttttcca atctcgtttt aaaaatatgt caattgcaac catgcttagc ggaatagtga
    54781 cggcttgcac tttggagaaa taagaaaaaa cagaacagag aagggcaata aaataccatt
    54841 tttttttgct gttttttgtt tgaatgtatg taagataact atacaatgtt gccaagaaaa
    54901 acaagccata caatacatct tttcgttcgg taatccagct tacagattct acgcgcattg
    54961 gatgtatgcc aaacagcaaa gcgccaataa atgctgctgt tttgcttatg cctaggcgat
    55021 taaaaatcag aaaaacaaag agtgtacagg caagatgaag gcacacatta ttcaagtgaa
    55081 aaggaaatgg attttctaaa ggatttggtg caaagaaata ttgctctacg gcaaacgtaa
    55141 aaatcggcaa tggattgtaa ttgccgatga catgtgtaga aaatatttgt ttcacagccg
    55201 accaactcca tgtagtagca aatggcttaa gtgaagcgtt ttcgtacaca ttaaaagcat
    55261 cgtcccaatt gacgaaatca gctgacaagc tgggcagaaa gacgatgcct gtaatcagta
    55321 tcaaaacaag aattgcaaga taagtatatt gctttgtatt ggcttttacg gcaggttttg
    55381 gtgttggttt cgatggaatt gatttcggct tatttgcttt ttttgacatc tttgtttttg
    55441 tcttttattt gaaaaaatta gcctccacaa ccaatcaatc tgtttggatt tttctttacg
    55501 agctcatcca attcaggcat tagcacaaat ggcaactgca tgtcaacagc agcaatctct
    55561 ttgtgttcgt ttgtcgcaac tgtgctattt tttttaggaa taattgtaat caatttcttt
    55621 tctttcctag attttttcat acttaaaatt aaacatttat ctttataatt atatgcatcg
    55681 cttcttacaa cgatatactg gattcttgcg taacattcgc attttacatt atctctataa
    55741 ttttcttcat caaaaaggat taaaacacaa ccagcactta tatccatttt ttggcattaa
    55801 caaaagtatt tatagcagca tttcgcataa agattttatt ggaaagaagg caaaagcacc
    55861 ttggttagat gcaattgtaa gcgaaaaaga cattcaaaat catcctgaat ttggttgctt
    55921 tccgacaaac attgaagcac aattaatgca atggaaaaaa aatggtttta tcatttggga
    55981 aaatttctta gatacagaaa ccgtcgatat tatcaatgga gaaatagatg cactgctaga
    56041 gaaaaatagt gttggattta attatacgaa tactaaaatt tttaacgcct ataagcatgc
    56101 tttttctatt agaaaaataa tgaaagacaa gcgtttgcta caattatttt catttatact
    56161 caatagaaaa gtcattccat ttcagagtat aaattttttg aaaggaagtg agcaacgtgc
    56221 acattctgat gcgatacaca tgacaacgtt tccacaaggc aatctgttgg cggcatggtt
    56281 tgcattagaa gatatcaata tagatcaagg tcctttgtcc tattatattg gttctcacac
    56341 tttgccatat attacgaatg ctgactatgg aaacagtgct aacaaatgga tgcttgatgg
    56401 cgaagccaat gagaaatatg aaaaaaaaat agctcaaatt atccaagaac aacatttgca
    56461 aaagaaggtg atatcgataa aaaaaggtga tgtatttatc tggcatgcta atttattaca
    56521 cggtggcgaa cctcaattaa ataaggactt aacgcgcaaa agtatggtcg tgcattattt
    56581 tgcttcagat gttatagctt accatgaaat tagtgagcga cctgcaatat ttgatgtgga
    56641 attggtcggt gaaatcgaag atgattttta caaaggacaa acgcatttgc tttagtcatt
    56701 ttttagaact caagaatgtt ctataataca tataagcgac tcctggaatt aatattatat
    56761 acagcttaaa ataggacgtt gcactcatgt acgtttttcc ataaacataa acagcaacaa
    56821 taaacacaat aaaaacaatc gtcagaattt ttgcgaaaat agtttcgtaa aaagtttttt
    56881 ttctttctat ctccaacacc ggaacctcag tatgtattga tgctgtagct tcttgtttac
    56941 tgcgttgtgc ttgttcatta tgcagttttg ttttctccag aaatacagta agaaatgcac
    57001 caaaggcaat gctctgttcg ccgtaattat cattaaattc ccaagtatcg ccattcttaa
    57061 aataaatcgt tacaaactga tattcgccac gatatcgacc tttatctgtt ccacttttgt
    57121 aactttttat atcattccaa gtgtatattt tattgctgtt ttttctaaac agggcactct
    57181 ttgatgatag atttctttct atcgttttat ctgtaatggt aaacgttgct tcgctcatca
    57241 gtaaataaat gattaatgcg attaatccta ctaatgctac agtacttaac atttctggag
    57301 catcaaaata aatcaaaaca tacataacat tcagcgctat gccacatgca atagcaacgc
    57361 ttgcccatat gccaaatggt aaaattctac aattgtattt aactagtatt cctgacatgt
    57421 gttaccacaa ctcttcttcg ctaatgtctg ctgctgggtt gaactggaaa agtttgcgcg
    57481 tttctatttt ttgtttttgt cgagctataa ttaagtcagt aattatttct gctgcatttt
    57541 cgcctttatg ttctatcatc agcttgcgca ctttatcttc atgcacatca attctataca
    57601 gcaaatataa caagtgttca aaatcgttat tgatccaatt atttattttt tcgataataa
    57661 aatttctgaa ttccgaaaag gaatggaagc taatgtcgtt ttcttctaac aactgcaaag
    57721 tatattgttg aatttttaat tcgtcttcca taaaaaaaat aattgcaaaa aaatcttatt
    57781 tattcaaaca cttacagcaa tacatcaatg gtcattgccg ttttttatac tataattttt
    57841 tctattccca taccaaacaa tgcaaaatca aatctcacag gatcattttt atcaagcata
    57901 cttaagttac gcgttaattc tgtaactgtc aaccaatcgc gttgttttct ttgtattaaa
    57961 tttagttgtc tcgcataatt ttctacatgc acatctaatg ggcacatcag catagctggt
    58021 tttatttgct tccacaatcc aaagtcaaca ccttgcttat cacatctaac catccatcgc
    58081 aaatacatgt ttaatctttt gcatgtcgat ttgtttgctg gtgacgaaat gtgtttctgt
    58141 gtacgctgtg gtgccatttc taaagcaaaa aaagcttgtt tgaattgttg caatccatct
    58201 tcaatagtat tggcaacaaa caaagtttcg agcgacttat tttttgaata ataatgatga
    58261 agaaaatgga taaaatacaa tacgtccgtt gcattaaaag tgcgatgcac aaaacttgta
    58321 aaaggtttga gatcttttgg tgaatgatgt aaaataaaat cgtgtggatg attgtccatc
    58381 aatttcatta ttttattaca actatttata attgcttttc gatttcccca tgccaacaca
    58441 gcagcaaaca agcccgcaat ttcaatgtct tgcaatgaag aaaaacggtg aggaatggaa
    58501 ataggatcgg cgtcaataaa atttttatta ttgtatttgt cgtgatagaa atttagtagg
    58561 tctatggtgt gttgcgaaat catatgcgtt tatctttatt ctctgctgct tgctcacttg
    58621 catttttaag aatatagaat actttttttc ctgctatcca aacaccaatt atggaaagca
    58681 atgacaatgc aggaatgttt ttttgaaaaa gaaaaaagtc atatgcgccg tgcaataatg
    58741 ttggatagac taaagcttta cgaatcaaat catctttatg tgtgatatca aattttgctt
    58801 gtccaatata atagcccatt acaatagcaa aaattgcatg tgcaggtatt gctgtaagca
    58861 tgcgcattaa tgctattcct attccacctt cagctacgta aaatatattt tcgaaggcgg
    58921 caaaacccat gcctatcata acagaataaa caatgccatc aagcggttca tcaaattctt
    58981 ttctacgaaa taaaatatag cgcaagaaga aaaatttagc aaactcttca ctaagcgcta
    59041 ctaataaaaa agcatgaatg agcgtattga tgatgctcgc atttgtacct agaatgctct
    59101 cgccaattaa tgtagtaaca cccgcaggaa agatgctaaa aacaccaaag aaaaatgcca
    59161 acaacatgag ccatatgggt tctttatcaa acctatcttt gtgtataata tatccacaaa
    59221 tggctaatgc tggtgcaatg gctaatatca ctaaagtata actcataaat gtcgatgcgc
    59281 taagcttgtt aataaggagg ctttagtgtt tatccaatag cattttttaa atcttcaatt
    59341 aaatcttccg catcttcaat accaacactc aatcgaatta aagaatcgac aacacccgtt
    59401 tttaagcgct ctgtcaaagg aatgctagca tgtgtcattg ttgctggatg gccaattagc
    59461 gattctacgc cacctaatga ttctgctaat gaaaataaag ctgttgaaga taatatctta
    59521 ttggcggctt caatactatc atcttttaga gaaaaagata tcatcgcacc aaaatcacgc
    59581 atttgtcttt tagcaatatc atgtccttta tgcgttggaa taccaggata atttacactt
    59641 gcaatttttg gatgtgactg taagaaagct gcaatttttg ctgcattttc acaggcgcgc
    59701 tgtacgcgaa tatgcaaggt cttgatacct cttaaaatta aaaaacaatc ttgtggaccc
    59761 ggcacggcac cacatgcatt ttgcaagaaa tacagttgct ccgcaagtgt tttatctttc
    59821 acaatcacag caccatgaat cacatcactg tggccaccca aatattttgt tgcagaatgt
    59881 acaacaatat ctgcgcccaa gtctaaaggc gtttgtaaat agggagaagc gaatgtatta
    59941 tcaacgcata ctaatagatt gtgttttttt gccaatgcac atactgcttc aatatcaaca
    60001 atacttagca ttggattggt tggtgtttct atccaaatta attttgtttc agcagtaatt
    60061 gcattttcga tattgacaat atttgtcata tctacgaatg aaaaatccat accaaatttg
    60121 ccaaatactt tggtgaaaat gcgataggta ccgccatata aatcattaca agaaatgata
    60181 tgatcgccac tattaaacag cttagcaatg gcgtctgtag cagccaagcc actggcaaaa
    60241 caaataccgt attttccatt ttctaaggcg gctaattggt tttgcaaggt atgtcgagta
    60301 ggattatgtg tacgtgcata ctcgaatcct ttatgcacac ctggtgcgtc ttgtacatag
    60361 gttgaagttt gaaaaatagg tgtcataatt gcacctgatg tagcatctgg ttctatacct
    60421 gcgtgaatta ctttggttgc gaatttcatt gtatcttatt tagttttgag tatcttgtac
    60481 ttatttagat ttattttcaa agttaccaaa taaaaaaccg cttgcaaaag ctacaagcgg
    60541 ttttaaatct tattttacat aaaaactatt accgcttatc ttcgatacag cagcattttg
    60601 tatagttttt agttgctttc atccaatgaa gttaaataaa gtatttttat tattcaacta
    60661 ataaaccata aggtctgatt tttttatttt tcaatttcca aaaaagacag ttcttcatct
    60721 tgtacaagta acacaatttc ttccctttta gaaattaatc tattggctat ttttcttgta
    60781 ttgaggttcc tgttttgaat agtacaacct ttggtggaat attttttagc aataccaaat
    60841 tcttatagtc ttcatcaaat ataataagac tgatacatga attatatcga taaagtcatt
    60901 ttgtaaatat ttcttgatgc gataagaaaa attctcatca atcatcaact tcatgctgac
    60961 actttaagtt ttctttcttt ttctgctgca aaagccacgc atgctaatat ttgttgctta
    61021 tttagttgag gaaagtcttc tatgatttct tctatcgaca tatctgatgc taaccaatcc
    61081 atcaatcatt caaaacattg atgactaagc ccgatttaag ctttggctca aaccaagttg
    61141 attttggtgg cataatattt cctgtgtctg cgatggcaat taattggtct aaagaaactg
    61201 gataaaaagc aaatgctatt ttcatttcgc cagaatctac tcttttttgt agctcgccta
    61261 agccacgaat accgcccaca aaatcaatgc gtttatcggt acgttggtct ttgatattta
    61321 agatttcatc taaaatatat tttgataaaa ttgttacatc taacacaccg attggatcgt
    61381 tttcattgta tgtattttta ttggcgttta gtttgtacca attgccattt acatacatcg
    61441 aaaattcgtg tagctgtgtt ggtttataga tagtttgacc aattttttct atagtaaaat
    61501 gctgtgctat tttttctaaa agtgtagctt ctgataaatc attcaagtct ttgaccaagc
    61561 gattataatc ttgtatttgt aactgattgc taggaaacaa acacgacaag aaataattgt
    61621 aagattcatt gcctgtgtgg tttggatttt cttcacgtag ttgcttccct acttttgccg
    61681 atgctgccga gcgatgatgg ccatcggcaa tatagcttgc cggcacctgt tctttaaaga
    61741 gcgtcactaa tttctgaatg atattttcat tgtctatttt ccaaagttga tgccgcacat
    61801 tttgcgaatc tacaaaatca ttttctgctg cttgatgcga tgtataatgc tcaataataa
    61861 catcaatttc ttcgacagct ttatacgtca aaaacaccat gcctggctga gccattttgg
    61921 ttttcatgtg tttaattctg tcattctctt ttgcgattaa agtgagttcg tgttttttta
    61981 ttttgtcgtt aaaataatcg tctatggaac tgcctgccac taagcctgtt tgcgttctgc
    62041 cttccatggt ttgtgcatac acatacatac aaggtttttc atcgcgttgc aaaatgtttt
    62101 cgtttataaa agtagtaaat acctctttgc ctttttgata aacttcctct gcatactgat
    62161 ctatattatt ttcaaaatta atttctggtt tagaaattct taaaaatgaa aatggattgt
    62221 ttgttgcctc tgcttttgct tctgcagaat ttaaaacgtc gtaaggcttt gcagctactt
    62281 tgtcaacgat gtctttcctt ggtcttaagc ctttaaacgg tctgatgttt atcatttttt
    62341 ttcaattggt ttcttgttgt tagaatactt tttcttttta tatttctttg tagttttttt
    62401 aatttttata ggcgttgctg ttgctattcc atatttttga aatgagccat ctgtaaaggc
    62461 gtgtaacgct cttatataga ttggattttg aatattggtg acttgataat atgcactact
    62521 attccataag ttctgtgcca ataatccttt tagctggatt tgtattgtgg atatgctttt
    62581 actataattt tcggcagaat ctggttctat tttttttgat tttgcatatt cataaaacga
    62641 atgcaagata gtagagtcaa catgaaattg cttgatgaag atatcaagcg ttggatattt
    62701 tttttctaat gttgttctgt ttttgtcaac atacgacaag acaaactgat tgaaggagcc
    62761 agagcgcact agtttaccat aaaacttaga agaccaagaa gtgtctaatc ctacaaaaat
    62821 atcaggtgtg atgcctccac cgccatacac gattcttttt tgattggtat aatattttgt
    62881 agaatctata attttcactt ttgacgagtc aaataattcg ccacttttat atcgctcttc
    62941 caagtcttct tgatatactt cattaccttt ttcataaggc ttttgaatat tgcgaccgct
    63001 tggtgtgtag taatgcgcca tggtcaggcg aaccgcagac atatcaggta aagagtagcc
    63061 tttttgaaca agccctttgc catatgttct tctgccaatg actaagcctc tgtcccaatc
    63121 ttgcacagcg ccagaaacga tttctgaagc agaagctgtg ctttgatcta ccataattac
    63181 taaatcacct ttttcaaaat tgcctttcgc ggtagcaatt aaatcaatac gtggctgtga
    63241 gcgaccttcg gaatagacga ccagtttgtc atcgcccaaa aacaaatcag aaagtgtatg
    63301 tgcttgatta agatagcctc caccatttcc tgttaaatcc aaaataattt tcttggcgcc
    63361 taatttcttc aaagaatcta ttgccgattt tgtttcctct gtggctgttg ctgaaaatcg
    63421 ttcgagtttt acatagccaa tttctggtgt ggccatatac tgtgcaccaa tcgcaaagat
    63481 tggaattttg tcgcgaataa tttcaaattc taataattcc ggttcgccac tgcgtttcac
    63541 ttttatcgtc accttggtgc ctttttttcc tcgtaagttt ttaaaaacac catcgttatt
    63601 aatgcctacg cctgctactt ttgtagtatc cacatacaca attttatcgc catcttgcac
    63661 gcctactttt tgtgatggac cacctgctat gacatgtatt acttgtatag tatcttgata
    63721 aatttgaaac tgaataccaa ctccttcaaa attgccttcg agtggttcgt ttgccttttt
    63781 aatttccgta gcattcatat acacggaatg tggatcgaga tgtttgagca tttcggtaat
    63841 gcctatttct actaaagagt cgatgtttgc tgattgtaca tagtaattat caatcaagtt
    63901 taaaaacgtc gccatctttt gctgatacat ttgcgcttgt gaaaagtaag ctgtaaaaag
    63961 taagcaattg actgtaagga ttaattttct cataactgtc tatgagtggt aaaaataaga
    64021 ttttgcttac aagaattatg ttatactatc ttattttttt cttcgcgaaa cggtagaaaa
    64081 aatagaatat ggcaaaaaaa tgcacacaag tatatttgaa tactagttgt ctatcgttgt
    64141 ggcaacaaag cgtgcaacaa ttattttatt attttgctaa gatgcctaat aatattattg
    64201 ccaatagcat cagaaaatat tggtaggctt ctacctcttt tggtaatggt atttgttttt
    64261 tcattattta tggtatttga aatttaagtt ataccataaa ttttaaattg tcaattgttt
    64321 taatgttaaa taagcaaata tgtatcgtgt gcaaatgtgg ataagtgcag atgaaataga
    64381 gaaattattg aaattgacat gttgtctttt aaaacaaact aagaacggtc gatgccttta
    64441 tattgtaagt cgtgttggta ataaaacaaa tcccatttgc catttacata ttctaatgcg
    64501 gtcaggtttt cgacccaatc gccactgttt aagtaggtag cgctaccatt ttcgttgctg
    64561 actatgcgat tttgtggttt gtgtatatgt ccacaaatga tataatcata gccttcttca
    64621 ataccaattt ctatggcgac atcttcaaaa tcttggactt tgctttttac aatggatttt
    64681 actttgtctt tgatatattt tgaaataaag attctgtctt taccatattt cattcttaca
    64741 ttattgacca atctattaat ttgccaaatt ttgtcgtagg ttctgccgcc tagttttgcc
    64801 agccaacgtg cagagccacc cacagataaa tcgtatttgt cgccgtgaaa aatccagtgt
    64861 ttttttccat cgatttctaa aatcaattcg tcgcgcaatt gtaaggtatc aaatttatag
    64921 cctgaaaaat tgcgcatgtg atcatcgtgg ttgcccgtaa tataatatac aggaatacct
    64981 tgccttgcca tatctaaaat gagaaaaaca ttttcgatat gttctttagg aaaatagcca
    65041 gctccaaaat tccaaatatc aagccaatcg ccattaataa ttaaaacaga agggtcgatg
    65101 ctttgtaaat agcgatttaa ttctgctgca tggcaggcac gcgtacctaa gtggatatcg
    65161 gaaatgatac agatttctat ctttctttta gtgtctgcca tatttttcta aaattaagca
    65221 gtaaaaaaat aaaatctatt tattttttat taaataagtg tgtctttatt tcaattaatc
    65281 gattttacac aataaaagtc ttaaaattaa tttaaccaat aaatttttta tgtttttttg
    65341 aaattttacc atatttgtag aaagaagttt aaaaaattat tcacatttta aaagttataa
    65401 catgtcatac gcaaaattag agtacatttg gctagatggt tacaaaccca cacaaagcct
    65461 tcgcagtaaa acacgcatag aacgtgattt cagcggaaaa ttagaagatt gtccaacttg
    65521 gtgttttgat ggctcatcaa cagagcaagc gcctggtggc tcatcagact gcttattaaa
    65581 acccgtattt atctgtgctg atcctgcacg caaattcggt tatttagtaa tgtgcgaagt
    65641 tttatcgcca gatggcacgc cacacgaaac aaacggccgt gctacaattg aagataatga
    65701 caacgatttc tggtttggat tcgagcaaga atatttcatt tgggatttga aaaaagacaa
    65761 acctatcggt tggtcagatt ttacagtgac cggtgagcct ggccctcaag gaccatacta
    65821 ttgctctgtt ggtggtggta aagccgtagg tcgttatatg gtagaagagc atttagactt
    65881 atgcttagaa gctggtttaa acgtagaagg cattaatgca gaagtagcaa ttggacaatg
    65941 ggaatttcaa gtatttgcga aaggcgcaaa agcagctggc gaccaaacat ggattgcacg
    66001 ctatttaatg gaaagagttg cagaaaaata tggctatgcg attaactggc actgcaaacc
    66061 acttggcgat acagattgga atggttcagg tatgcatgct aacttctcta atgaagtgtt
    66121 gagaacatgt ggtagcaaag aaacgtacga aacgatttgt aatgcgtttg cgccgtttgt
    66181 gaaagaacat atcgatgtgt atggcgcaga taatcacatg cgattaacgg gcaaacatga
    66241 aacacaatct atcgataaat tttcgtttgg tgtttctgac cgtggtgctt ccattcgtat
    66301 tccaattgca acagtggaca gaggttggaa aggctggtta gaagatcgcc gaccgaactc
    66361 cgctgcagat ccgtataaag tggctgcacg tattgtaaaa acagtgaaaa cagcaaatgt
    66421 gtaatcattg aattatataa tagtcaagtg gctaatctca ataaaaaatc cgaccaatgg
    66481 gtcggatttt tttataaaat aaggttgcgt gtgtttttat aaaacaataa ctgttactac
    66541 gtgtttgcaa ttattattgt aattgttctt gaaaaaaagc actcatactt tcaaaagctt
    66601 tttggccttg tgctgtattg aaaaacaaat gaggaatgcc atgtggcaaa ttataccaat
    66661 gagtttctaa atttccattc aattctgttt ctagttgtct aactttccaa actgggcaaa
    66721 atgcatcgac ataagcaaaa tgaccttgta cttttttatc tattaatgaa gaacaacttt
    66781 ttggcatgcc gctgcctttc atgcctaaag aatgatggaa tacaacgggt gctttgactt
    66841 ctggtcgcaa cgcagcatgc agcacaaaac cgcctgttag gcactgtccg acaactccta
    66901 aattattttc atcaattaaa tctaaacttt ttactgcttc aattatttca aaaaactcat
    66961 cgagtggtgc gttgttgttt gcttcattgt tcctagctat actactcatt aaaaattgca
    67021 tacaatattt acgcaaggaa gagccgccgc tatataaatc tggaatgatg acatggtaat
    67081 cgccgtcgct acatagaatt tctgcacttt tgatagtagt tttctggatg cctgacaaat
    67141 ctggcaagaa aataattgca ggtcttattt gattggaaga tgcttcaaaa aaataagcat
    67201 cgattgttcg tttttgtaat gataattgta ttttttgcat aatctaagat aagcaataaa
    67261 aatataagca attatttttt gccgacagcg attacggaaa gtccactctt attcagtaat
    67321 attttatcta ccaatttaaa gacaaggacc aatttattaa acaatgacat ttgtccagct
    67381 ggcactgttt ttttctttaa aatggagcct gaaaaaaacc aaccaaaaat gccaataaag
    67441 ttgaagtatt gcgagtgttc tattttaaaa tgttttccta ttaatgcatt taaagtagta
    67501 tttgtatatc ttctgtaatg ctctaaaaca acatcaaatc gattatataa ggcttgatag
    67561 gctggcacta ggattataat gtttcctcct tcttttaata atttttttgc attgcttatt
    67621 gcaaggctat cgtcttttat atgttcaacc acattcaagg catacactgt atcaaatgtg
    67681 ttcatcaagt gtgcgaattt gatgtcaaag ttcgtatcga ccaaatcaat ttcgagaact
    67741 tttgatgaag ggaatttttg taataataca tcacaataac tcgttctgat atcactaaat
    67801 gttatatcaa atttatcgcg taaaaaataa cgagatatat tgccaatgcc actaccaatt
    67861 tccaagactt tgcctttaca aaatggtttt atagtttgat acatccattc gttgaaatta
    67921 tctgctttat caatagcttc taaagtagtt tgtccgtctg cgtcaatttc tttaaagtga
    67981 aagtcgtaca atgcgtcttt gttttattat gaatgaaaaa aggaacaaat atagtatttt
    68041 taatgccaaa ataaatttac gaaacgctaa agatgtttga ttttttgaaa gaacgaaaat
    68101 tgagtgtgtt gctcttgctg gctttggccc tgcgcctctt atttttttgg gtttaccgac
    68161 cgtgggatcc aagtgttgaa agaaacttag tattaaacgg tgatgccgta caatatcatt
    68221 atttaggaat tgcgatatta aaaacgtttg gatttgcaga caatgttttt cgtactccag
    68281 gctatccagc atttattgcg gtagtgtata gcttgtttgg cataaaacct tatatcgttt
    68341 tgctgattca aattgtgatt agtgttgcca gcatatatta cgtttataaa ataggattgc
    68401 tttgttttaa taaaacagtt ggatggtttg ctgctgtcat catggcttta gatccgcatc
    68461 aaataacgtt tgccagttgg ttatttgcag acacattttt tgcatttcta tttatcatta
    68521 gtatttatac tttcgtaaaa ggaataacag aaaagcgcat taaatatatt gtcatcagcg
    68581 cagtcgtttt agggtttaat attttaacca aaccggttat tcaattattt ccagtttgtt
    68641 ttctgctttt atgtttggta tatcaaaagg ccgatctaaa aacacgacta aaatatggat
    68701 ttatttatct gttgatacca ttttttattg ctctaccttg gttgctgcgc aataaaatca
    68761 aatatcagca ttttgcgatt tcatcgatag tagggtttga caagttggtt tatagtgttc
    68821 cgctgacgga acattcattg acacaaaaaa gcaatggtca aataattgac agctttttga
    68881 caaaaataaa aattgaaaat cctcagttga agaatatacc agagaattgc accaaaatat
    68941 ggctgaatat tacttttgaa gctacagatg tatatggaaa atatgcagac gaatacttag
    69001 ccaagcaccg aatagcgtat ttaaaattgc atgtaaaagg catgttgaag ttaatgctaa
    69061 acatgggaac acaaaacatg ttggacaaac tacacattga aaataaaaaa tgggacgatg
    69121 aaacacgtta tacttcaggg ttttttcaat tagcaaagaa atttgtgctc accaaaacaa
    69181 agacagaaat tatacttgga ttgtttattg ttggattttt aattgtctgc tatgtttatg
    69241 gctttatagg tgtctattat tgtctaaaaa acaaacacgt attttggtta tttctttttg
    69301 gtggtgctat tttatatttt ttactgattt atggtaagtt gcccatagta cgattcaagc
    69361 tacctattac tgcgatgtat agcgtacttt ctgcctatgg attttattgt tttcatgtat
    69421 tgaaaaaaga gaaattatca tagttcattg ttgtttttgg acacaaatga ctttgtaatt
    69481 tcgttcaatg cattcacttt ttcttcaaag ctacctttaa tagaactcct atactcatga
    69541 aggttttcaa gtaagtcatt aatgttttct ggtaaaattg tttcaaaaaa ctcacgcaat
    69601 cttttagaca aagttggcga ttttccattg gtagaaatag ctattttcaa atctcctttc
    69661 gtcacaatac tgcctaaata gaaatcacat aattcaggtg tgtctgccac attaaccaat
    69721 actttccttt gtttggcatc aaggtaaatc tggtggttca attctttgtt cgcagtagcc
    69781 gcaatgcaca actgcatatt attcaaatca ttttcttcaa atgctttctc catcaaaaca
    69841 agcgttttgt ggttcctgct ttgctttcta atttctgctg aaatctcttt tgctaccaat
    69901 gtaatatttg cattcggaga atttttcaga ataaatctta ttttttctgt agccacactt
    69961 ccgccaccta caattaaaat attgatgttt tctgttttaa taaatattgg atataaagta
    70021 ttcacttgaa aatttaagaa acagaaattt aggttttatt tattttacaa gcatatatct
    70081 ctgcacaatc gacaactttg ccaagtatta taattgctgg agctgtgatg ttttgttctt
    70141 gtacctttag ataaatattt gacaattggc cacacacgac tttttcattg gcaagtgtgc
    70201 cattttggat aattgcaatt ggatagtttt cagttttata ttttgtaaac aattgaataa
    70261 tttctgcaag cttaccaacg cccattaata tgactactgt tgccgtcgat tgtgctgcta
    70321 gttcaatatc ggcagacaat tcaccacacg atgtagtgcc tgtggtcacc caaatacttt
    70381 ctgtaatgcc acgtttagtg agtggaatat tttgtgccgt tgccaatgcc gtagcactcg
    70441 aaatgcctgg aattacttct gttacaaagc cgtgcgatgc tacaaattcc gcttcttctt
    70501 gtccacgacc aaacacaaat ggatcgccac cttttagacg cactacagtg ccatgttttt
    70561 taatattttc gacaatacag gaatttattt cgtcttgttt catataatgt gcgcccgctc
    70621 tttttccaca aaaaatagga atggcattgg gcgcatattg taatagctct tcgcttacta
    70681 gggcatcata catcaccaca ttggcttgtt gtaatgcttt aattcctttc aaagaaatca
    70741 gatctggatc gcctggacca gcaccaacaa gtatgagttt tttagtcaat ggttgtcttg
    70801 tttattttgt ttaattctta tgctttgtaa ctgctttcca ctactacttt gtctttgttt
    70861 gctgctgctg tacgatgagc aataacatct tttaaaaagc attcaaattg ttggacatag
    70921 tttgttgcaa atgctatttc tggttcatgc tggttgattt gtaatactac attttcaaat
    70981 ccgttttcca ttttaaattc acctgttgca accaagtgct catcaaaatc ttttagcatt
    71041 ttaatttgtg tattacactc tatttctttg cttaacagca atgcttttgc gccaataata
    71101 aaacctgtat atgcatagta tatcgcctct ggaaatttat tttttaataa atgatcttta
    71161 gcctctatta atttattgtc ggcatcttta ataattccac caaccatatc atacatcacg
    71221 ccagcacatt cgcctacgcc aatttcttgt atataatttt cgacgccacc atagtcaata
    71281 aaatcatcat cgacatatag agacacattg gctaatggtt ttagtaaagc ataaaaataa
    71341 tttttgcctt gtcttctata ataatcattg aagtattcac cttcattgct attgatttca
    71401 taatcattca gcacgatgcg caacatttcg ggcgaacgtt ttgtcggaat ttttacaatt
    71461 ttatcgccaa cagtgccgac gccatgtaca tcaacgcctc cacctaagat aacctgcata
    71521 gctggtgcca cacgctctcc tacttttata gaactgccgt gaaggccaat gtttgctgcc
    71581 atgtgctgac cacatgagtt catgcaacca cttattttta tttttatttc ggagtcaaaa
    71641 attaagtgat aaaattcatc gtagatgaca tcttctaaaa cggtgcctaa ggtcatgcta
    71701 ttggttacac ctaaattaca agtgtctgtg cctgggcaag tggttacgtc ggcaatcgta
    71761 tcaaaaccta cttttgcaaa tcctagttta tgtaattctt ggtagaaata tggaagcgac
    71821 gcttttggta caaatttcaa caacaatcct tgattcatag taatgcgcat gtcttctgat
    71881 gcatattgct gtactaatgc ggcaattgct cttgcttcat caggttttac gtcgcctttg
    71941 gttactttta atgctactgc gaaatagcct gcttgttttt gttcaaacac atttgttctc
    72001 aaccaatctt gataagcttt ttcatcttcg atagtaactt gtggtgcaat attgtggtgc
    72061 aaaggctcgc ctatgctcac tttcgtcgtg tctattttat atgactttac tttattggcg
    72121 gtgcgttctg ctgccaccaa ttccataaat ttttctaggc ctaagtcttt aattaaatac
    72181 ttcatacgtg ctttatgtct gcgcacacgt tcgccatatc tatcaaaaac tcttagaaca
    72241 gattctacaa aaggaattat ctgatcttcg tgcaaaaact catacgcagt ttgagccaaa
    72301 atagattgtg cacctaaacc accgccaaca actactttaa aaccacgttc tccattttct
    72361 attcttggta taaaccctac atcgtgcagg aaagtatatg ctgaatcttt gtcagaagag
    72421 gaaaatgcaa ttttaaattt tctgcccata tcttgattga ttggatagcg caagaaaaac
    72481 tcgaatgtct ctcgtgcata cggcgacaca tcgaataatt cgtctgggtc gatgcctgca
    72541 atagatgagc ctgtaatgtt acgcaccgtg ttgccgcacg cttcgcgagc tgtggcgcct
    72601 acatcttcca agcctgccca cattgccggc gcatctgtta ctttcacatg gtgtaattgt
    72661 atattttgcc ttgttgttaa atgcaaatta acacttgcat attgctcaga taaatcggcc
    72721 aatcgcaaca actgtgctgg tgtgattttg ccgaatggaa atttagtacg aaacatctgc
    72781 acaccaagtt gacgctggcc atacacacca cgtgttaagc gaaaagcttt gaatttgtct
    72841 tcaggaatct cgccattttt gtaggcattt actttttgtt gcagttcaaa aatgtctttt
    72901 gctgcttctg gtgtaatgtt ttttgtaatg gtaatgctca tttttttttg cttttattat
    72961 tgctttcgca acacatccgt ttatttataa aaaaacctat ctttttagag ataggcattt
    73021 aattttatat aaaagtataa cctatccgac tactgcgaac aagaacaaca attgataaat
    73081 aggctgacta ttaatttcat ttgcaactaa aattataaaa aaaaacagaa aaaaaaggct
    73141 gttaatgatt tgataattga ataaaaattg ttctattcat tttccgccac tacttcttgt
    73201 acctcaggta cgaagcgttt tagcaagttt tcaatgcctt gtttcagtgt tactgtcgag
    73261 gatggacagc cactgcaaga accttgtaat tgtagtgtaa gtttgccatc ttcaaagctg
    73321 acaaaagaga tatttcctcc gtccatttct acggctggtt taacatattt atctaaggcg
    73381 cctttaatgc gaatttccac atcactatcg tcgtcgttaa ttgtgttggt cgctgttttc
    73441 gtaggtttta caaaattatc gcttaaaatg gtttgacctt cgtcgatata tttttttaaa
    73501 aagtctttta taggtatcat catttcatgc cagatgtgtt catctgtttt ggtgatggtc
    73561 acaaaattat tggtgataaa cacattgctt acaccatcaa atccgaataa agcttttgct
    73621 aatggagctt ctgtagtttc ttctatgttt tgaaattcta ctgcatcatt tagcaataag
    73681 gctttgttgg ttacaaattt taaggtattt ggattgggtg ttgattctgt atatatcatt
    73741 accggtttta ttgttgtatt gtccatttta tattgttttg aataatgtta taacatttac
    73801 ttttcttgaa agtttattta ggcatttttt tgagtaatcg tagctgttgc ttgtgctgct
    73861 ggcaaaataa atatctcttc gatattgacg tgagccggct gagtggccat aaaataaatg
    73921 ctgtttgcca catcttctgc ttttaatggt tgatagcctt catagacggc actggctttt
    73981 tgttcgtctc ctttaaaacg cactaatgaa aattctgttt ctgctgcgcc tgggcttatt
    74041 actgacactt tgatatgata tggcaataaa tctacacgag cgctgcgtgt aatagcgcgt
    74101 actgcatgtt tggtgccaca gtaaacattt ccgccaccat atgcttccca gcctgctaat
    74161 gatgctacat taatgatatg tcctttttta cgcgccatca ttaatggcga cacttcgcga
    74221 atcatataca gtaagccttt tacattggtg tctatcattt gttcccaatc gtcgtaaaca
    74281 ccgtcttgta ttggacctct gcctgctgcc aagccagcat tatttaataa tacatctata
    74341 tttttccaat catttggcaa tgacgcaatt gcttcttttg tttcttgaag atttctgaca
    74401 tcaaaacaca agctaattac acgtaccgaa tagttggata taagctcttt ttccaatgcc
    74461 ttcaagcgtt cgtttcttct gccgcaaata attaaattcc aattgttttt tgcgaaaatt
    74521 tgtgcacttg cttccccaaa tccggatgtt gctcctgtaa ttaatattgt atttgacata
    74581 tcagaaaata atagacaaag gtaaggaaaa ggaaaggaat ctttttttct aaaagaaaaa
    74641 cttactcttt atataacaaa tatgtagagc aatctttaaa tggcattttc tttacaaatc
    74701 catatttatt tgggtattgt ataaaatgct gccacgtact gtcatttttc aatcgtaaat
    74761 cttgcaacaa gaaatctgtg accaacacat tatcgacttt tctggctttc aattgctcga
    74821 taaatgtttg ttcgttgtta taatcttgtt ctgcactaaa atcagtataa tttttgtctg
    74881 ccatcatgct caagtttaaa atattactaa agatgacgtg ttggtgttga ttgcgttcat
    74941 tcagcatttt tatcaatact ttatacgatt gattagggca aggtccgctt ataatacgtg
    75001 gtgttttata gctttgcata tctggcattc gccaaaagaa aattcccact aatacaaaaa
    75061 tcgaaactgt acgaatatgc tttatgtcaa ataatatttc gaatactatt gacaatgtat
    75121 agagcaaagg aattaataaa aataagagat aatgtggtct tggaaaaaag acagatgcac
    75181 ttgccaaacc tggcaatgca aagacaagta aaatgagcaa ccattcgcgt tctgctgtca
    75241 cacgcgacca aaaaatttgg cgtttctgtt ttggaatcaa cgcaaataat ggcaatatga
    75301 taagcaacac aacagacaga atttctaagt attttctata aaaaagaaat tttggatata
    75361 gaaattcaga cagcgatgca aggactacca aaagaaatat tattgcatta gtcagtattc
    75421 cagtaatcac tgttaatgga tgatttttta ctatatccaa aaaggtatta cagtttggaa
    75481 attgtttttt gctaaatgca atccaatcaa taaacaatgc ataatccagc ttgttttgat
    75541 aaatatattt tagtgtataa tgctgacaga aaacgccata tatcctatct tttcccatgt
    75601 aggtatttgc tggcaattgc acaaaaacta aaagcagcat gatgataatt ataaatggaa
    75661 tactaaaaac taaagtcgct ttaaatgttt ttgattgata gatataatac atcgtaaata
    75721 ttaaaaacaa gaaaaaagac aacattaact cagggcgtgc aaatgcagca acaaatagca
    75781 caaaacaaat aagaatgtat tgcttggatt ttgatgtaat gaagagttta ttaataatta
    75841 aacaccacaa aaaaagagca acaacaaacg cagaaatacg aggataattt acaatattga
    75901 cattggaaac caacatacat aaggccatcc acatgctcgc aataaaagaa atgccataac
    75961 tgtataaaaa ggcaaatagg gtactcgccg taagcagtat tattatttta tagttcaata
    76021 aatataactc aattggccta tgctcaaaca aagaaagcag tttgtaccac atattataag
    76081 ttggacccca atcgtttttt atttgtgcaa acatgtccag tccataacgc atatatttca
    76141 cttcatcatc gaatgaaata tcgaccaact gattaaaatt tctgagcaaa cgaatgccac
    76201 aaagcaatag caaaagcatg cctaaacaaa gtttagcata actaaattgt tcttctgttt
    76261 tatgaaaaaa aaatgctttt acggcagtca gcattatgga aaaaggtata aaaaacgaat
    76321 atagcagtat tttacactaa ttttaatcaa attaataaaa atgacactgc aagaagcaca
    76381 acaaacgata gacacttgga taaacacaac tggcgttcgc tattttaacg aactgacaaa
    76441 tatggcaata ctcacagaag aagtgggcga agtagccaga ataatcgcca gacaatatgg
    76501 cgagcaatcg tttaaagcat ctgataaaaa caaggaactc gctgatgagc tggctgatgt
    76561 tttgtttgtg ttgatttgta ttgccaatca aaccggcgtt aaccttactg atgctttggc
    76621 taaaaattta gcaaagaaaa caattcgcga cagtgagaga cataaaaaca acgagaagtt
    76681 gaaatagaat ttctcattca ataatctatt ttaaataatt tatagcatcg caatactgct
    76741 ttttgatttg aacgctgata cactaaaaat aaacaaaaca gcctttttgt catttattac
    76801 ataataaatg tgtcattttg tctgtttttt tgttttgaat tgatgttttt gtctgttttt
    76861 tgctgatgga ataggctttg cagcaatgtt attgtattta ttcatcaaca aaaaaacaaa
    76921 ataatatgag aacattagta aaaacatcat gcggcacacc aagcaataat cgctttttct
    76981 ttgacgatgt atttggaaaa gatttttttg attttgcacc agctttcaac ggcaaaaatg
    77041 tgccagccgt aaacgtaaaa gaaacagaaa cggaatttag tgttgaggtg gcagcgcctg
    77101 gttttcaaaa ggaagacttt agtattacct tatatgaaaa catattgagc attgctgtgg
    77161 aacaaaaatc ggaaaaaaat gaaaatgacg ataaggctaa atttagcaga aaagaatttt
    77221 catacgctgc ttttaagcgc agcttcacga taaacgagga cagcattgac acggaaaaaa
    77281 tagcagcaaa atatgaaaat ggtattttat tgctccaaat tcccaaaaaa ggaaaaactg
    77341 aaattgaaaa aaaggaaaag acaattagtg tggcataata atagtggttt ttaaagaagg
    77401 aagcctgtag tgtgtttgct acaggctttt taatttttaa aaacggctat tcatctgtgc
    77461 atataaaagt agcgatttga aatgtgagaa aaatctttct acagcatttt ttctaatttc
    77521 tctgcatatt gcgagttcat agattttaat tcttccagca ttttttttgc ttgttttgtc
    77581 tttttttgct tgacgaaaac ttgccctaga taataacgca ccaagtccga tttcgagtcg
    77641 tattgcaatg ctttataata gtcagattct gctgctttat agtttttttc tgaataatca
    77701 atccagccta attcaatcca agcatctgca aattttggat cgatttctac ggctttgtat
    77761 aaatatggtt ttgcttcact atatgcttct ttttcgttta aaatccaagc aattctatag
    77821 ttggtaatgg cattattgtt ttcaatggca aagctctttt tgtaatattc taatgccttg
    77881 tccaaatctt ctaattcttt gacaccataa tacaaatcac caacattaga caaataaatt
    77941 gcattttttg gattgaggtt ggatgctttt atatagttat ctaatgccat cgatttttta
    78001 ttgagctgac tgtatgcata cgctaattcg ttgtaagcag gtgcatattc gctatcgagt
    78061 tttaatgacg cgagttcgta gtcaactgct ttttgataat tcgcttcttc attcatgata
    78121 tatcctaatc tataattcga aaaggagaca tctttagatt tgtctaagca ttgctcataa
    78181 taatctttcg ctttactaaa ttctttattg tcaaaatata catctgcaat gccttttaaa
    78241 gcactgtgtt cattagcatc tactgacaac gcatttttat attttatgat agcttcttta
    78301 tagttgccaa gcttatagta ggcataacca aattcgtata gcaatttagc attcgttgaa
    78361 gtttttatac cattagttgc tgtttttaca gcatcatcat acaatcctaa ttcattctga
    78421 caccaagtaa tatcataata agttgtcccg ttttttgggt tcaattcgct ggctttttta
    78481 taccatttaa gcgccatttc aatatcgcca taatcttctg cttctttggc tttttctaat
    78541 gctgattttt ctgtttcttg cgcaaatgta atttgttgcg ttaaaaacaa tagcattaat
    78601 aagtacatca ttttattcat tgtcttttgg tttttacatt ttaaaattaa tgaatatatt
    78661 gagttgttac aaataataat agcattcggt agcaagaagc aattgcctaa aaataaatga
    78721 cagttttgta acaaaaaatc taacgctatt ccgcttaaaa ttaatggtcg caataactcc
    78781 tatattcgat tttttaagta ttttacatct taattaaaaa caacacacca actatgatta
    78841 tccaaacacc tttagaaaaa ctatatcact ggactgcaca gaaaccagat caaacatttt
    78901 taagacaacc ttataatggt gaatggattc atttttcatg gaaaaaaaca gacgatgagg
    78961 tgcgcagaat ggccgcttat ttgcgttcct taaatttgcc tccacaaagc aagattgcaa
    79021 ttatttcaaa aaattgtgcg cattggatca tgagtgattt ggccatcatg atggctggac
    79081 atatttcagt gccactttat ccaactattc cagcagattt aattcgctat tgtctagagc
    79141 attgcgaagc gaaggtggct tttattggca agttagatga ttggaaaaat caacgtgcag
    79201 gtttgcctga tggtgttaaa ggtatttctt atccattttg gacagaagaa ggttatgaaa
    79261 attgggacga tatcatagct aaaacaccac catttacaga aatgtacgtt gctaaaagcg
    79321 aagacattgc gactatcatt tacacttcag gaactacagg cttgcctaaa ggcgtcgtcc
    79381 aatctgttca cacattttcg tatgccgtat gttgggcact caccacactc gatgatctga
    79441 cagataaaga acgatttttt tcttatttac cattatctca tattgccgag cgtatgttag
    79501 tggtcatggg cggcctttat tcaggcggtt ccatttcatt tgcagaatcg ttagatttat
    79561 ttgctgcaaa tttgaaagat accaaaccta ctattttctt agccgtgcca cgtatttgga
    79621 caaaattcca aatgggtatt ttgtcaaaaa tgtcgcaaca aaaattagat gtgttattga
    79681 aaattcctat tataaacaac atcatcagaa agaaaataaa agaaggactt ggcttagaac
    79741 atgctaaata tttattaaca ggtgccgcgc ctttgcctgt caacacaatg ttgtggttcg
    79801 aaaaattagg ttttgttatt tatgaagcat atgctatgac agaaaactgt gcttactcac
    79861 atttaaacag aaaaggcaaa agaaaacctg gatatgttgg cgtagccatg ccaaattgcg
    79921 atgtgaaaat ttcggaagaa ggcgaagtct tagtaaagag tgaagcaaca atgattggat
    79981 attacaaaga gcctgaaaaa acagccgaaa ccattactgc tgatggatat ttgtgcactg
    80041 gcgatcaagg tgaaatcgat gctgaaggat ttttaaaaat tactggtcgt gtaaaagata
    80101 tttttaaaac agacaaaggc aaatatgtcg cgccagcgcc aatagaatta gaattgtcta
    80161 aaaatgcgat gattgaacaa gtttgtgtag taggtgcaaa cttaccgcaa accatggcgc
    80221 ttgtggtatt gtctgctgat gcaagaaatg aaagtgacga cacaattgct aagagcttag
    80281 aagaaaccat gcatgatgta aacaagcttt ttgacaaaca tgaaaaaatg aaaaaaatta
    80341 tcgtgatgcg cgaagaatgg accgtagata acaacctatt aacgccaaca ttaaaagtaa
    80401 aacgcaatat tttggagaaa gccaaaaata cacattatga aaagtggtat aaagatgcca
    80461 atacgataat ttgggaaaat taaaaagaga aaaaggctgt caaaattgac agcctttttc
    80521 gaaaaaaacc cgatgtttta tttattctat ttcaatattt tttacataag ctgccaactt
    80581 cataaacaca taatctttgc tatttaaggt tgctgtatta taacgccatg gaataacttc
    80641 taaataagcg attccatcag agcctgttgt tgttaatgct gataaatctg aagaattgat
    80701 gacaatattt gttgctgtgc cgccatatct ttttattact gtgcctgtag aagtagaact
    80761 tccactcaaa atagcaacat aaatagagtc tgcattggaa gagctaatgt ttaatgataa
    80821 attgctcgac ttagtaacgg tagtaggcaa agttgctaag ccaccataag aaggaaatgc
    80881 aacattgtga ttatacgtaa atgctgtaac gctagacgaa ccagccacgt tccaattgct
    80941 acctgaagaa aaatttaaat ctacatttgt tggcgatgtt ggcgaaaatc catttctata
    81001 atatgcatta ttgtcagctt ttgttagcga aaaagtattt acatcaacgg cgcctgcgtc
    81061 tgtaagtgta gtgcttgatg tagttgtaga gaaagaagcg accgctgttt ccaaatcaat
    81121 attcatcatt ggcaatgcta caggcaactt tactggcgaa aatttattcg gatcaaattc
    81181 aaaataagta cgaatactcg ttaatgtgcc ataataagca ccaggcgctg taatcgttgg
    81241 cgttggtggc actggattag tagttgcatt gttgattatt tttttacaac tagttgtagc
    81301 aaataataca aacaaaaaag aaacgaaagg caaaagaaat tttttcatag atttaatttt
    81361 ttataaaaat atatcaggtg acaatgcatg catctacgtg ttttgtggta ttttttttat
    81421 taaaatgcat tatttgacag aaaatagcat tcattgtata gcacaaacgg tgaattagaa
    81481 ataaaaaaag cgatgttctt atcacatcgc ttttacaatt atatcacaag aagattagaa
    81541 cacgtatcct actttcacaa agccaccatg tatgttttgt ttaaagtctg aatacaaagt
    81601 ataaccacca ttaatggtaa atgatgcatt aggttttgct ttaatcaaaa tacctgtgcc
    81661 ggcattcagc atcaattgca aatcttttaa ttgcttcgtt tcagtttcag gcgcattgcc
    81721 tactttatta ataccagctt gcactacaaa cattggtgtt acttttttgt ctaatacata
    81781 agctcgaaaa tctgctgtcg tgtgcatttg ataaatacga ttattagaca cttgaatacc
    81841 aacaccttga ccaataaaaa agtatggatt aatacgcata ccgattgctt gataaaggtt
    81901 aaaataagct tgtttaccag caaatgctgt tcctacttct gtaatcgaag aaaatcttct
    81961 gtcatattca acatattctt ctgctgttgc ttcgctattg ttttcaactg tgatatttga
    82021 aggttcaatg cttacatcat taccattggc gaatgcagtc atgctaaata aagccattac
    82081 tgctactaca aattcttttt ttattttttt attttttaag atttacaata tttgtttaat
    82141 gtttttaatt gttcaaaact ttcaaacaaa attgaaactt tattttcata aaactgttag
    82201 tttgtagtta tgctttactt gtttaatact tatgaaaaga ttgtgtaatt attatccaga
    82261 actttcaatt attactgaaa gtttaagttt gtgaaaacat ggtaataatt acattgaaaa
    82321 acatttgtaa tatgttgatt atcaatgtaa ttttgtaaat aaaatacaaa attaagaaag
    82381 tgctaataaa atagaaattg ttatggaaaa acaaaacatt caacatttat acttctacct
    82441 tgcggtgctg tgcgcattat ggtttgccag cacttcttgg atttggacct atctgtttgc
    82501 gctttttata tcttatccaa ttggcttgtt ggcatttatt ttttggtatc gaggcagcaa
    82561 tattgatgag aacaaaaaca gatacaatct accaaaatac attttaatat ttggcttact
    82621 tagttcaata atttcattct tattaatgct ttaaaaggaa caaaaaaaat gataagttat
    82681 gcaatacctt ctgtgtatcg tttaaaatta tctaaaatgg cttgccagcc tgcttgttgc
    82741 atatctaatg gatggtgatc ttctggatcg aaggtttctg ttatgtgcgt ttgtatttcg
    82801 tcgccagaaa atagaatgtt gacagtacga ccatcgccca tggtgtagct aattttttgg
    82861 tgtaggatga cttcatcata tataccacca aaatcaaaac caaagcttcc atctttggct
    82921 tccatgcgcg tagtaaacga accgcctgct tgtaggtcat tgctagcact tggtgtgtgc
    82981 caatcttctg aggcgctaca ccattctgta atatgtgctg gcattgtcca gcaattccac
    83041 actttttcta ttgaagcatg tatagttgta tgtatagtaa tgcttgtatg cttgttttcc
    83101 attcttgaat ttaataaaaa atctggagca gtggctgttt tttacgataa tgtcctacaa
    83161 aaccatgatt ttgttggacg catcaacata cttgacgaaa caaaaaattt atcagaagga
    83221 aatacatgta aaatatggtc atgctcattt ttgataataa ccgtattggc gactacttct
    83281 aattttccta ttatttcaaa actattcgat acgtttagca cttccatttt tatcggatca
    83341 aatgtttgct catctgtcca cattttatag gtgccgttga gtttatattt ttgaccaatc
    83401 ttttgtgcat actggtctga aaattcttga taatcatact cggtcaattc aattaaattt
    83461 ttcactttga ttttttgcaa accattttcc agtgtggtat aagcatattc caattgtatt
    83521 tgcaatgctt tttctgcgtc tgttttggca ttttgccaat ccattggtgt aatgttgttt
    83581 ttattcaaag caaaaagtaa atgctttaaa acaaaggctc tataatcttc atgtacgata
    83641 tgaagatgta atttatacca aggaaagcga tgattaataa tttgaaatgc ttgttcaaat
    83701 gtttcatatt cgccattctt gttaatggct aagccttcat ctgtaaaatc aatttcctga
    83761 tggtgataaa taaattttgt gcctgtgtta ctaatgatgc gttctatcga aaggctgcct
    83821 ccttctgcta atacttcgaa tacaattgtt gtcatactta ttttttatca aaagtaatgc
    83881 tcaactacga cacaacatgt cgtagttgaa aaaacaatat tagctattgg actgcaaaag
    83941 catgaacgta attagcaaaa ggaagatacc atgcattata aacaataatg ctattttgaa
    84001 attgattgtg ttttagtcat tattgattag ctattgagca ttaccagatt ctagatccca
    84061 aaaaaagaaa aagaaaaatc cggcgaccca ttcacgtcta gcattaccgc attctccaac
    84121 gcggctgtcc ttgtaataga catcaccatc ggattcgaaa actctatctg atgtttttta
    84181 tctttagtat attttttaat attacataac acgtttattc catattttaa aacacaaacg
    84241 cacatcttac gatgtgcgtt ttgctttgta ttatgagcga taggtgctaa tgttatagtt
    84301 tgcgttttac ttctacttgc tcgaatgctt ctacgatatc gttaacttga atatcgttgt
    84361 agttttttat tttcaaacca cactcatatc ctttggtcac gtctttcaca tcgtctttaa
    84421 agcgtttgag tgagtcgagt tcgccgctgt gtacaacgat gccatcacgt ataattctga
    84481 ttttgttgga acgtgctact cttccatcta atacataaca gcctgcaatt gtaccgactt
    84541 tactaacctt aaacacttcg cggatttcga tattaccaac aattttctct tcgaattttg
    84601 gttctaataa accttccatc gctgctttga tttcttcaat tgcatcgtag ataatgttgt
    84661 agtatttaat ttgtatgtct tctttttctg ctaatgcttt agcattcatg gaaggtcgaa
    84721 cctggaaggc acaaatgata gcgtctgatg ctgatgccaa caatacatcg gattcgctga
    84781 tagcgccaac gcctttgtga atgacattca cttgaatttc ttccgtagaa agtttcagta
    84841 atgagtctgc taatgcctcc acagaaccgt ccacgtcgcc tttgataatt aagttcaatt
    84901 ctttgaaatt gcctagcgct aaacgacgac caatctcatc taaggtaatg tgtttcttag
    84961 cacgcatgcc ttgttcgcgg atgatagtag cacgtctatt tgctgtttct ttaccttcat
    85021 tttcgtcggc ataaactttt attttatcgc cagccgttgg tgcgcccaat aagcctaata
    85081 cttgcactgg cgatgatggt cctgctttga caatacgaac accacgctcg tttgtcattg
    85141 cttttacttt tccatagtac tgacctgcca ccacgaaatc accgatttcg agtgtgcctt
    85201 cttgcaccat aatagtagaa acataaccac gacctttatc taattgtgct tcaattacgg
    85261 agcctacagc taatttattt ggattggcct ttaggtcgag catttctgct tctaatacaa
    85321 tcttatcgag taatttatcg atattgatgc ctttttttgc tgatatttct tgacactgga
    85381 atttgccgcc ccattcttct accaatatat tcatttgtga taattggttt ctgatgttat
    85441 ctggatttgc gccttcttta tccattttat taatagcaaa aatcataggc acttctgctg
    85501 cttgtgcgtg cgatattgct tcacgagttt gtggcatgat ggcatcatct gctgcaatta
    85561 caattacggc aatgtcggtt aatttagcac cacgtgcacg cattgctgta aatgcttcgt
    85621 gacctggtgt atctaagaaa gtgatgtgtt cgccgtttgt cgtttctact tcgtaggcac
    85681 cgatgtgttg cgtgatgcca cccatctcac ctgctactac atttgcactt ctaatatagt
    85741 cgagtaatga tgttttacca tggtcaacgt gtcccataat ggtcacaatt ggtggtcgtt
    85801 ttttcaaatc ttctggtgca tctatatctt cttcgtcttc atcttcttca tcggctaagt
    85861 tgataaattg tgcttcaaac ccaaattcac cagctactaa ttctattact tctgcatcta
    85921 agcgttggtt gatagaaacg aacacgccta aacccatgca agagctgatt acttgcgttg
    85981 gtgtaacgtc cattaagcct gctaattcac ttacagtaat aaactctgtt aactgtaaaa
    86041 tgtgatcttg tgcatcatcg ccttccgtac gtgcagcatg gtcttcccgt tttgattttc
    86101 tatattttga gcgatctgat tttgttttcg ttccttgtag ttttgccata gtagaacgaa
    86161 ttttatcttg gatatctttt tcagaaattc catcttttga ctctactggt ttaccnnnnn
    86221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    86281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctctt cttcaactgg ttttacttct
    86341 tcttttttta cttcttcgac aggttctatt ttcttttctt cttgctgtgc tttctcttca
    86401 ggttgctctt tctcttcttt tacttcgact ataggttcag ttttttctgt tgttttttca
    86461 acaacaggtt caactgctgg ttttacctct gcttcctttt tcttcttagg tttgtcaaga
    86521 tctatttttc cgacaatttt tggaccttct acttcgtgtt ttacggcaat aatttcttcc
    86581 ttttcttttt ctacttttgc tgtttctttt attgcttctt taatagaaat ttcttcttct
    86641 tttcgtttgc cgatattgat ggcttctgct ttttctttag ccaccatgtc gcttctaaat
    86701 tcgcgcaata ggatttcgta ctgctcttcg gagagcttag tatttggcga atttgcaaca
    86761 tctttaaaac cttttgcatg taggtggtca actattgtgg aggtacctac atttagttct
    86821 ttggctgcgc ttgctaatct atgtgtttta acttctgaca tatcttgttt ttagacaatg
    86881 gtcaatggtc aacagtcgat tggaaaagat gtactatgac caatgacata tggctaatga
    86941 ctgttcatgt tattcaaatt ctgtttttaa tattttgatg atatcatcaa ttgtttcctc
    87001 ttctaagtct gttcttctta ctagttcatc tctatctaac tctaacacag acttcgcagt
    87061 atcacaacca atacctttta agatatcaat aatccagcca tcgatttcgt ctgagaattc
    87121 gtctaagtcc acgtcgtctt cttctacggt atctgtgtct ctgaatacgt cgatttcgta
    87181 gcctgttaac ttgcttgcta atttgatatt aaaaccacct ttaccgattg ctaatgaaac
    87241 ctgatctggt tttagataaa cagatgcgcg tttttttgca tcgtctaatg ctataccttc
    87301 aatttttgca ggtgttaaag agcgttgcaa caacaggttt acgttattag taaagttgat
    87361 aatgtcaata ttttcgttgc gcaactcgcg aacgatacca tgaatacgag agcctttcat
    87421 accaacgcaa gcgccaactg gatcgattct atcgtcgtaa gattctactg ctactttagc
    87481 gcgttcacct ggctcacgaa cgattttttt gataacgatg agtccgtcca tgatttctgg
    87541 cacttcattt tctaataatt tagccaagaa agaaccatcc gtacgagaca aaatcaccaa
    87601 aggtgtgcta ccgcgtaatt ctacattttt gataacggca cgaatgctat cacctttttt
    87661 gtaaaagtct gtttttattt gttcattttt tggtaaaatt aattcgttgc catcatcgtc
    87721 gagcaataga atttcttttt tccaaacttg atatacttct gctgtaatga tgtcgccaac
    87781 acggtctttg tattttttat acacttcatc tttttccaaa tccaagacgc gagaaatcaa
    87841 tgtttgacga gctgctaaaa tcgcgcgacg gccaaatgat tctatggtaa tggcttcgta
    87901 taattcttcg ccaacttcca tgtctggctc gatggttttt gcggaagata tttctacctc
    87961 tgctaatctg tcttctactt taccgtcttc tacaatcata cgatggcgcc agatttctaa
    88021 gtcacctttt tctgtgttga cgatgatatc gaagttttgg tcggagccgt atttttttct
    88081 taataaagtt ttaaatacat cttccaacac gcgcatcatg gttggtctgt caatgttttt
    88141 aaagtcttta aactccgaga atgaatctac taattctgca ctgttcatgg caattatagt
    88201 ttaaaattaa aatgtttttt tactgatttt atttcatcaa aagctaaagt gtgttcttcg
    88261 atagtttctg atttttttac tttaataatt tcttttacgc ttatttcatt gcttgtagca
    88321 ctcaacaatt ctcctgttat cttcattcca tttttcaaaa ctacatcaac ttgtttattg
    88381 agttgttttt gaaattgttg tgctactttt agtggctcat caatgcctgg tgacgaaaca
    88441 tctaaagaaa tgttgtctgt catcaagtta ttttcttcta aaaagtgttg aatatgtctg
    88501 cttatttgca cacattcttc aactgtaata ttatttgtac tatctgcgta tatttcaatt
    88561 ttaccattag acagtacttt aatatcgact aaaaacaagt cattattttc gaaatagcgc
    88621 gctatttcgt tttctattaa ctgttttaat tgtactaatt tttccattgc tataagaata
    88681 aaaaagagga ctttgtcaag tcctcttcca ttcaattttc g
//
LOCUS       DAOQQV010000002        58690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_138_length_58690_cov_8.152554, whole genome shotgun sequence.
ACCESSION   DAOQQV010000002 DAOQQV010000000
VERSION     DAOQQV010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 58690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58690
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138_length_58690_cov_8.152554"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /gene="thiC"
                     /locus_tag="PLL59_00380"
     CDS             <1..1806
                     /gene="thiC"
                     /locus_tag="PLL59_00380"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMY22284.1"
                     /translation="TTPFPNSKKVYIQGVQHDIQVAMREITLSDTKLNGKTLHKNPAV
                     TVYDTSGVYTDENYTVDVRKGIPRIREKWILERGDIERLYEFSSDYGTQRMNDEALNH
                     LRFEHIKMPLRAKEGRNVTQLHYAKMGIITPEMEYIAIRENQRIAEYNASLNGQAAVL
                     CQQHAGESFGANTPKSFITPEFVRNEVAEGRAIIPCNINHPETEPMIIGRNFLVKINA
                     NIGNSAVTSSIEEEVEKAVWACRWGADTIMDLSTGKNIHETREWIIRNSPVPIGTVPI
                     YQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSR
                     GGSIMAKWCLAHHKESFLYTHFEEICEIMKRYDVAFSLGDGLRPGSIADANDAAQFAE
                     LETLGELTKIAWKHDVQVMIEGPGHVPMHLIKENMDKQLKECQEAPFYTLGPLTTDIA
                     PGYDHITSAIGAAMIGWYGCAMLCYVTPKEHLGLPNKEDVRVGVITYKLAAHAADLAK
                     GHPGAQYRDNALSKARFEFRWQDQFNLSLDPEKALEFHDETLPAEGAKIAHFCSMCGP
                     QFCSMKISQEVREYAEKGMQEKSEEFIEKGSEVYL"
     gene            1995..2213
                     /locus_tag="PLL59_00385"
     CDS             1995..2213
                     /locus_tag="PLL59_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22285.1"
                     /translation="MKSKTNNNKQEVRKLTPHDLAFGIGRPATIEELDLLMEDMENET
                     FTEATTVMKRISKNLQKKNQVKSVKGKV"
     gene            2194..2478
                     /locus_tag="PLL59_00390"
     CDS             2194..2478
                     /locus_tag="PLL59_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMY22286.1"
                     /translation="MLKVKYSKSAEKTIEGITDFVEQLNTKGAGERWKERFNRKIHKY
                     AQPISYALCRHLDFALKKYSCVSIENWVIIFKVKNDVFCVYGIIHSSLLK"
     gene            2488..3111
                     /locus_tag="PLL59_00395"
     CDS             2488..3111
                     /locus_tag="PLL59_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HMY22287.1"
                     /translation="MQVIIYTSPTAIENEIEIISQLFDCGLDYLYIRKPDLDDFSLVD
                     FVEQIPEKYWKRCISTSMIITKEFNLGGYHFTRDIVQKNEKYNDKILDWLHSNHKISS
                     VSAHKLEEIEKYKIKFNHVIVSPLFNSISKENHRYDWNFAELKNVLQNLKSENINHKI
                     FAVGGIDLDKIEIVKNLNFDGVGLLGAIWSNTDNAIEKFSTIKKQYE"
     gene            3114..3869
                     /locus_tag="PLL59_00400"
     CDS             3114..3869
                     /locus_tag="PLL59_00400"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HMY22288.1"
                     /translation="MSAIKKRPYVLSIAGYDACAGAGVLADVKTFENIGVYGLAVSTC
                     ITYQNDTKFEGVHWLSKKKIKQQLYPILDAYTIDYVKIGLIENLIVLGDVIQLLLSYN
                     PKIKIIWDPILRASAGFDFHKKLPKKISKHIFSAIDWITPNWNEISLLSSEKDAIVGA
                     EKLAIFCNVYLKGGHNIETPATDMVWINKKLDILHPKEITALSKHGTGCVLSSALTAY
                     LALGNTAHKSCELSKEYTYQFLISNNSNLGYHK"
     gene            3870..4484
                     /gene="thiE"
                     /locus_tag="PLL59_00405"
     CDS             3870..4484
                     /gene="thiE"
                     /locus_tag="PLL59_00405"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HMY22289.1"
                     /translation="MHISRFHYLTQDLPNISHQDLAEIACKNGIKWLQLRVKNKSLDE
                     YLFIAKEVKRITDKYKSTLIINDNVEICKEINADGVHLGKNDLAPLEARKILGSNKII
                     GATANTVIDILQHQNDGANYIGLGPYKFTNTKKNLSPVLGVEAYTEIFKNNNFQIPIV
                     AIGGIQLADMKILMNTGVYGIAVSSAINLSYQKEKTIADFLQLV"
     gene            complement(4456..6138)
                     /locus_tag="PLL59_00410"
     CDS             complement(4456..6138)
                     /locus_tag="PLL59_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22290.1"
                     /translation="MFYIKQHILQLLTTTQEKPIEKNRYQIAFSIFILLAAGIKLYYH
                     MQQYMDIQFADEAAYIQFGLALFEKMNRNWGPLYAVWYKCLSYFTTSPILLYYINFAI
                     TSIAMSLLMFIFLIRLHLTPIVALFISFCILVSDLNVSVWPRVSHFCLMICLISLIIS
                     SYIKPVYYKCIAFTIACLLCAYARPEFYLSFMIMLLVSLATLYVNKQFLSKNDFYILS
                     FLLLIIGLLHLIFRFPSNDFFGYNRGVAAFYQHYAWNYKMRTGATFDAWLNWEDLAKK
                     QFGDCNSILCVIKTQPGIFISNTLFNVRTYLLQLLKVCSFIFPIGLFYGKKKQLALTI
                     IVLFTIVVLLFRKKSRVIFIEKIKAYQFYLLLLFCFITPTLMSCLAVFPRDHYLYLQM
                     PFYTIILVCIAAYPFNKIKANKLSFTIFGVLLFLATPSIQSFSFMQFNGDKQTLCNKK
                     LVNFLENKYSSKPHTIFTNLPFVHGMLPNNFKEINTIFDKKKHMPFTHYVDSAKIDMV
                     IVLPSLLRDPHISSDSTWKNFITNYEQAGFKKEIFSDCDMYLLVKQAAKSQQ"
     gene            complement(6150..7799)
                     /locus_tag="PLL59_00415"
     CDS             complement(6150..7799)
                     /locus_tag="PLL59_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22291.1"
                     /translation="MHSWKSQINEDKQSVLYKCTYVLCILFLLFLGYKWQANLAAVMD
                     ILAADEAEYLRNGTALFDTIHKDWGPSYNIWYKALSYFADSKIELYYLNYKLTAILAT
                     ILLFVVLVRYNINLVVAFTISSLFFLSEINIETWPRVSHFVFICILIGLYISSFLKEG
                     TTKLFLFAAISIVAAYARPEIRPFSFAVYALALFVWYKNKTAFSQIRIMVFIAIILLI
                     FFQLVFGSPASFYRGNVDRMYIAFCQHVAVNEFLHHDKTVDAMAGWRVYANKVFPLCD
                     SFSCIVKTYPLAVLKNITYNIPNYLLSITSVLGSLLFPIRLIGKHKIFIGICVFIWII
                     IIGGILYKPFRIAIWNSIKNYKWQFIAIAIIGLPSMMTSVLIFPRMHYILCHLFLLIY
                     ILAFFLTQIANKIKLPILSIVPVFIVLFLISPKADEYRFFRDNHDLGNMCGQKYIQHF
                     NKDDSTKHIIFSDILNLSYMLPDNYSDYSVEYDFKDGMQFDHIRKQFNIDIVLVNENI
                     LKNPYLKKDSTWQQFLLHYENFGFEKKQVYNECAIYILQKK"
     gene            complement(7857..9086)
                     /locus_tag="PLL59_00420"
     CDS             complement(7857..9086)
                     /locus_tag="PLL59_00420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMY22292.1"
                     /translation="MAEDQKIDKSNPMYYLAGWRGVAAILIFMIHYRLKKYNLPSVLG
                     YTGTHSFFLLSAYFVSVGLFKKFEDKNINAWKTFKDFVLKRILKILPVYYLYIFVMLL
                     IALVLMLVFKLEAAQVDTFRELKQFGIGLFTYTYNFREVYNLFVLHKYVPESPTTYPH
                     LWYISFDLQLCIIIFLFIAFVRDRALLLKISVAGAIAMVIFRFIAWQYLLSIPGDDLS
                     REYIFEKIPLMQLDTVFYGFILCLYDFKKSKKLFIALIFAAVLCYGWAFYRAYLISID
                     TGELLRTTIREDIYVIPFYGIQVIDSTVAFFIFILFACIINFPEKFKLLLHPVLMRTG
                     ALSLSMYIFQFLFILIGMFLVAGILRKFLPGVIADVIGLFSFLVMDYFFAGIIFKFFE
                     RPIHKLMDRKIFKKENK"
     gene            complement(9137..10357)
                     /locus_tag="PLL59_00425"
     CDS             complement(9137..10357)
                     /locus_tag="PLL59_00425"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMY22293.1"
                     /translation="MADNDYRLKNIYLPQFDGFRGSFCFTILIIHHHFTYLNLPSNIG
                     YFTMHSFFIMSSFLITRTLMIDKQRSDSFKTFFFKFYIKRTLRIFPVYFAYLLFTVLV
                     GVLTYPTAAKSILGIIYELKYFGWMLLTFTYNFKDLYCLFTDLMYAKCMVFPHLWSLS
                     LEEQFYIIVPFMIYFMSEKAIKRLTIAMIIIFPIIRIVGFNWLCELTDDNMHRSFILY
                     RATIFQYDSFFYGTFLAIFDIKWSDKTMNRIFYAVLSIFILTIPVNGWIIHQQTGESF
                     MHIITSYDFMTRNGQYIYIDCLVNILCVVFFYICFKGENRFSFFRNKYLVDIGSRITY
                     SSYVYQYIFILPVFVYIFPYFFESLKVGRFLAELFSMILSISGILLLSHFSYNTMETY
                     FLKKKDKYLAKFKK"
     gene            10462..12522
                     /locus_tag="PLL59_00430"
     CDS             10462..12522
                     /locus_tag="PLL59_00430"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMY22294.1"
                     /translation="MQKAIVTGAAGHIGFHVTKVLLSKSYDVHLLVRSINSNIVELEA
                     LGAKVYPCNLFETQSYAALIKDADVLFHLAAENTTSMSNASRVLENTDKLTQVVLSAC
                     HENGVKTVVYTSSVVVIGRSPNAHQLLTEKDVNAFPESPYVLGKMQAEKYVVDFIQKH
                     NYDIRRVYPSWAVGPNDPRLTPPHKVIKDFLEKGAPVYFDGGVSIAAVEEIAKGHVAA
                     YEKGSFGETYILSGENITFKKLYELLVANSRQKMPLLKLPKFAIVLAAWIFTKLFKLF
                     GKEFPISPEYAKAVAGSYSWYDCNKAKSLLGYQTRPVEEILKNAVHDANRKILGVHLL
                     GKKTHLANSTHITQSDDINTNAAGTLLITGVPGWLGNRMIDILINGDRFGQHHSNRKV
                     RVLLEPRFKGLLHLPNNYEIVYADITNKEQVHAAVKGVHSVFHLAGAIYPKNIDVLYK
                     VNEEGTKHLVDACIANKVRRFVFMSTDSTCGHGTKDQAIFDENTIATPYKNYGQSKYN
                     AEKYILEKTKEGKIDGTSLRGFWFFGPFAPARQLTFVNMFHWPRQIVFGNGKNLRSIS
                     HTDNIIEAFFQVENNPATYGSWYWIGNDQPHPTVNEIYASIAKALGVPYKPFYIPKFF
                     CRCFEIADTFLGKFNYLHPTIHAAGKFDYDIAGNIDKAKRDFGYAPKVSLDDAAKELK
                     DMTN"
     gene            12707..13480
                     /locus_tag="PLL59_00435"
     CDS             12707..13480
                     /locus_tag="PLL59_00435"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HMY22295.1"
                     /translation="MQSQLQIADKLFSSRLFLGTGKFGSPTLMHDAVLSSETQLVTMA
                     LKRVDLKTQSDNILTYLRQTKVHLLPNTSGVRTAKEAVFAAQAAREALETNWLKLEIH
                     PDPRYLLPDAVETLKATEELVKLGFVVLPYIHADPVLCKRLEEVGAPAVMPLAAPIGS
                     NKGLETKEFLRIIIEQSKVPVIVDAGIGAPSHAAHAMELGADAVLVNTAIAIAENPVQ
                     MAIAFKMAVEAGRLAYEAKLAKVSDRAHASSPISSLLED"
     gene            complement(13655..18964)
                     /locus_tag="PLL59_00440"
     CDS             complement(13655..18964)
                     /locus_tag="PLL59_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY22296.1"
                     /translation="MHKLLQALGSLLFNNSCHKLHAYAFANTQSVILKKECINRKIRI
                     ASLLFILLASLNFQTALAQLACIAGGNQISYSDNSWTGYVYDGTSFNTYKGYVTESAN
                     FNRVWGNTATSRPACATDANSFSIRYLMRLPNLAAGIYKFTIGNDDGKRLAISNTLIT
                     YFSGLTYQINAWSSCCADHSAIYTHNGGDLYLIFEFYEGGGGAQSNFSYCYEGPLFSY
                     GTSSWNGYAFNATNLDIAQYKGTITGGYANNSTDNLGISWGTPLTSTACGGAIGSDNF
                     STMHAVNKSFTEGFYRFKFTSDDGIRLSSNGATNPYYTNDWNIYTNWQSSSTVDDART
                     LYLNGSKNLVIQQAEGGGGAGVTNTICQIPGDKSFNQSYPNWKALVYDGDEPTVDNNY
                     MGHFTTTGSDPNLIDNNWGNGQPGFTTGSGNCARSMDNDGYAVRYLTTKNFSNGVYVF
                     STQNNDDSRRLSVDGGNTWHLSCWHCGNGNTISDPIELTGNKNLVFDMREGSGGARAA
                     LQQCQMDAGGTNGSLGNATAVGTSTWNLYYYNTTDYSFPISSFRRTNTGGTSAANSAS
                     LNLSFPGVPTGVGQNCGTAMNDNFSLKALLTRTFTKGIYTIQSGADDWSRIRINGSSF
                     INEHGSCCTTQSTNVAFDGNTQALEYQMRETGGPGSTTVNISCSDAVAGTLSSNQACN
                     STGAVTLSWNGGLGWNQLEYSNDGSTWANVSGHTESARLNTGDVTKTWSSINPTTTTY
                     YRVRTTSCNGTFYSNVVTIVRASAFTGNVTIANNITLGGNISINGNFTLNSGITITVP
                     QGCPLVINATNITINGTINANNSGELGGNGGVQGGYWSSEGNNTDGRGILWCSDKDNC
                     RELKTIGGGAGGNGAGAGGGNGGSKGNDGQGKKQRCSNWDDEGGRVGGSGGGGGGKGG
                     GNGGSGGNGGNGSTGNGTDACREAGCSLIYAVGSGGNGATSGGNTVGSASDYTALPGS
                     GGGGAGGGGTGRDNRTAGANGGNGGGSISLIACNTFTMSTSANISANGSNGGNGGNGG
                     DWTETGECCGDLAGGCDERTRTGRGGAGAGGGGGAGGTILISAFGPISIPAGATLSAN
                     GGNGGAGGTSDVNGQSGGGGGGGRVKIFYNPCVSFSNAATIAVNGGTGQNAGNNGTSS
                     LYNHPSYSTLAAGAITSPTDVKICYGTNPISNINADASTGGANNSALSCTAVLPTYEY
                     QWYVTSTACNNPTTGTSSTPNANWNVISGATSNNISAAQLLSGINIVGNTTTVGTYCF
                     QRRTKSSTCYAWTDKVSITIRPQLNPSANAAVIPPCANPNQPYAELIATPPITDVSGI
                     WTIVSGDGTFNDATNPSTNITGLSSTGNTTLVQWTLFYTDNTPACSTSLATPISIAPT
                     ALDFNTVSTQSTSTPQYYNCKTCTVKNGNSYSYYDNAGKIMAKVIDPTGGSIEMGSTE
                     LCLGYDYNANSTSPLVTNVKSVLTNFGDLQPYLTRYWSISPNTKTGQDVIVSLFFTQA
                     EYDALKLKASGTAYEFADKNELVITKYDNGSNGTFTNPPNYPATNSSAKLIFPSITRY
                     PNTTTGPDYAATFTLNSFSTFYLHPNRFPFAALPVELISFSGVYADLKNNLSWKTASE
                     RNANRFEVEKSNDGINWYYIGEQVAMGNSNGQSNYYFQDNHPITGNNYYRLKMIDNDA
                     TFEYSNVINITVDEVVSDGIVSVFPNPVSDNVTVIIQATENHTTDIQISNVLGQHISI
                     KPISVQKGLNEIELSFQEMSAGAYVVSYIDNNGNQHQHKIIKK"
     gene            19311..20360
                     /gene="porQ"
                     /locus_tag="PLL59_00445"
     CDS             19311..20360
                     /gene="porQ"
                     /locus_tag="PLL59_00445"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HMY22297.1"
                     /translation="MERYFSILLCVLLSNIVYAQIGGKNVYDFLQFPTSSRMEALGGG
                     VQSIKYAKDADLSMAISNPCFIDSSFHKQLAFTNAFYTDKTNIGNIAFAYYHPKIKSA
                     IAYTFQFASYGKFDGRDIAGNSTGTFRAADFNLQVGIGRSIRRFYYGVNIKFIASHAE
                     SYTSLGFATDVAIGYHHPKSNWTFAAIVRNAGVEMKPYIKESGRQKLPIQLDISFSKR
                     FKKLPLTLSVTAHNLQTWNLKFPEEEQTTLFGTTKKKSKGAEVVDNIFRHLSFGAEVQ
                     AGKPVRLRLGYNHLRRQELGVGKKKGFAGISAGFGVNIKQFAFDYAYAQYHRTGSDHQ
                     LSFRIKLDEFGKKAK"
     gene            20393..21076
                     /gene="cmk"
                     /locus_tag="PLL59_00450"
     CDS             20393..21076
                     /gene="cmk"
                     /locus_tag="PLL59_00450"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HMY22298.1"
                     /translation="MSNKINIAIDGYSACGKSSTAKRVAQRLAFLYIDSGAMYRAVTL
                     YFQQHLIDLDNTLAIEQALPKITINFKKVGDVHHTFLNDVDVENEIRQPRVAALVSEV
                     AAMSNVRKKLVEQQQQMAHQKGVVMEGRDIGSVVMPDAELKFFMTADVDVRTKRRQDE
                     LLKKSGKLFSLEELKANLLHRDKIDSTRADSPLTRVADAIEIDTTHLTLEKQVDLIVE
                     KALERMMLN"
     gene            21178..22269
                     /locus_tag="PLL59_00455"
     CDS             21178..22269
                     /locus_tag="PLL59_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HMY22299.1"
                     /translation="MESSIKEIKIDSTSDDAKTKIIRKEVQQASKEIRLKYPILQHQN
                     TIGFFLFAFSIAMILLTAYLYLSNAIGFVITTISVAFWTSILHELEHDLIHFMYFKKN
                     KFIQDLMMLGVWVFRPMTINPWLRREIHFHHHRVSGTRTDIEERGVTNGEPWSFKRFI
                     TTADIFLGGVLRGNVIRKDIIDAVKKGELPKAQALEFKKVKLYGMLPFTLLLYAIWYV
                     FLAHYTIHFITQLMDISYQSPAWLQSQFTWINPLVAILIFPNLFRMFSLHFITSNMHY
                     YGDVECGNVMQQTQVLNKWYFIPFQLFCFNFGSTHAIHHFVVNETFYVRQLTATTAHK
                     VMREQGVRFNDIAAFRRDNRFYEKQLHPA"
     gene            complement(22280..23050)
                     /locus_tag="PLL59_00460"
     CDS             complement(22280..23050)
                     /locus_tag="PLL59_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMY22300.1"
                     /translation="MISAVIIEDEPIFQEILKNAIKETGFSIRVDGVCNSKREAKKIL
                     PSVRPQLIFLDVELADGKGIDLLNELESTDFEIIFTTSHDKYAISAIKNNAADYLLKP
                     IKEKELKKAIEKVAKRLEEKETLKQAEQLQGYLDKIKQEQQQDSKLMVPTKDGMIFLK
                     VNDIVRLQSESNYTLFFLVGNKKVLVAKTLKNFEEKLLSYNFLRIHQSHLINLAHMKE
                     IDHGDNLAIMTDGSKVEISKRKKKEFLDSLEKNNKTIA"
     gene            23125..25194
                     /locus_tag="PLL59_00465"
     CDS             23125..25194
                     /locus_tag="PLL59_00465"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMY22301.1"
                     /translation="MTSKFKIKASSLSDDEVRELYAKAAVCKQEKNYIDALDFYNKII
                     DYKNSEECVECAKVYEDIGDIYFELKDFQKAFENLEKALHIYADNEEYELQLNQYKKI
                     GSLQQGIWQFKKAIEIFHHGLSLATRLDKKEKVLEFELLLGNVFNWADQLEDAEQYLV
                     SAIEKEKRINSPLIKLRAHVSYAILLRKMKQFQKSETYFKMGIQLSSENNNAYMMDIT
                     KSYGVLKYETGHYEHAEELLVKAEKSAEFEGNDATRAVIFEYLAMLYEYKKDYEKAYH
                     YINKFYGRKLELLEKGYSDDNNILQAKIGLETAKRERVIAEETAKAKSLFIATISHEI
                     RTPMNIILGTAALMLNDSPKPAHLKYLQTLKKSGENLLGIINDILDVSKIEAGKLEVE
                     YEPVELSTLFDEIISILEQTAHEKQLSLSYTVDEKINFAIMSDPLRLTQIITNLISNA
                     IKFTAQGSVHLSATLLPNDKLQIVVRDTGMGIPKDKLKTIFDQYEQVRTKVQKKYKGT
                     GLGLAIAKKLVEMMQGNIEIKSKINIGTSFIITLPIEKAAIQQKVDIATLKRDAAFLS
                     DKVIVIVDDVEDNRTVIRETLKFFNPAVVTHEAADGQQALDLLLQLTNVHLVIMDLDM
                     PVLNGFEALSAIRKHKKIKKLKVLASTASLITTSDDEFIEFGFDAYLPKPFEIDSFFL
                     LLEKFLQ"
     gene            complement(25226..26014)
                     /locus_tag="PLL59_00470"
     CDS             complement(25226..26014)
                     /locus_tag="PLL59_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22302.1"
                     /translation="MENQLLDSDMLSNKEGKSLSSLLTNGYDTNAVDYIKKGFEIFKQ
                     NIGPFIGFIVLVAVMEMIISMIPFLKGIVFALIAPIIAGGFIVAKMVDKKEVHSFNNF
                     FDGTKNYMPLLIVTLIPTLITAALMLLLGGWAYFKMAFLGITPKINISNFGDISAFSQ
                     SMTGFAGRAGLVGIASIAISVLFLFATFFVLFEKMEPIKALDLSRKLISKKFFNWLGF
                     LLLLGLFNAAGALCLVVGLLVTIPSSICALYVAYEDVVGLNLRD"
     gene            complement(26124..26516)
                     /locus_tag="PLL59_00475"
     CDS             complement(26124..26516)
                     /locus_tag="PLL59_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HMY22303.1"
                     /translation="MKQDISNRNDIEQVIILFYKLTFEDERLGKIFKEISPLHLETHI
                     PLVTDFWEGILLDAANYRGNVTEKHFDTNKRTPLTKADFDLWYYYWEQAVNTLFEGEL
                     AEKAKFRARSIADIMSYKMDYVNAIPKG"
     gene            complement(26546..26983)
                     /locus_tag="PLL59_00480"
     CDS             complement(26546..26983)
                     /locus_tag="PLL59_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22304.1"
                     /translation="MRHFALLLLFCLGLSACKLIDTEIACSEFYVKNDLSSTVFIRNY
                     KIIDSGFYFSIDSFSVAAGETKLINEDCRYVDSIIPYPNGNFTKLFFVNGKAKADTFS
                     YNGSIYLHDSINIYNKNRWIISGTKKELKAIYTITALDSLEAI"
     gene            complement(27128..29899)
                     /locus_tag="PLL59_00485"
     CDS             complement(27128..29899)
                     /locus_tag="PLL59_00485"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HMY22305.1"
                     /translation="MSIYNDYIKEIDERKEQGLHPKPIDGAELLKEIIEQIKDLSNAN
                     RDASLQFFIYNTLPGTTPAAGVKADFLKDIILGNVVVNEITPAFAFELLSHMKGGPSI
                     KVLLDIALGADTAKAEQAANVLKTQVFLYEADTERLKAAYESGNAVAKEILESYAKAE
                     FFTKLPEVPEEIKIVTYIAGEGDISTDLLSPGNQAHSRSDRELHGKCMITPEAQAEIV
                     ALQAKHPAASIMLIAEKGTMGVGSSRMSGVNNVALWTGKQASPYVPFVNIAPIVGGTN
                     GISPIFLTTVDVTGGIGIDLKNWVKKVDADGKPMLNENGEPILEEKYSVATGTELTIN
                     TKKKKLLNGDRELIDISKALTPQKMEFIKAGGSYAIVFGKKIQTFAAQTLGIESTPVF
                     APSKEITIKGQGLTAVEKIFNKNAVGVAAGKILYTGSDVRVKVNIVGSQDTTGLMTAQ
                     ELESMAATVISPIVDGAYQSGCHTASVWDKKAQANIPKLMKFMNDFGLITARDPKGIY
                     PAMTDVIHKVLNDITVSDWAIIIGGDSHTRMSKGVAFGADSGTVALALATGEASMPIP
                     ESVKVTFKGSMKEYMDFRDVVHATQAQMLQQFAGENVFQGRIIEVHIGTLTSDQAFTF
                     TDWTAEMKAKASICISENATLIESLEIAKSRIQIMIDKGMDNRNHVLRGLIEKADKRI
                     AEITSGENPALRPDANAKYYAEVVVDLDLIAEPMIADPDVNNKDVSKRYTHDTIRPLS
                     FYGGTKKVDLGFIGSCMVHKGDMKILAQMLKNIEAQNGKVEFKAPLVVAPPTYNIVDE
                     LKAEGDWDVLRKYSGFEFDDSAPKSTARTKYENMLYLERPGCNLCMGNQEKAEKGDTV
                     MATSTRLFQGRVVEDSDEKKGESLLSSTPVVVLSTVLGRTPTIEEYTAAVKGINLTKF
                     APSHKLLVK"
     gene            30092..30523
                     /locus_tag="PLL59_00490"
     CDS             30092..30523
                     /locus_tag="PLL59_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22306.1"
                     /translation="MPTFSTRKAIEKAITLFNKHAYNAGLDFEVYTSDTHCLYVQASF
                     DFAYYVNVDIECREVFYTNIASNAKWPDAWSNDQLFLLDDEELESILNFNEISVADDK
                     TVFGIVFNIIGKENYHINGLVICSSLYFNWRYPNYNDQYLM"
     gene            30636..31301
                     /locus_tag="PLL59_00495"
     CDS             30636..31301
                     /locus_tag="PLL59_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22307.1"
                     /translation="MKKSIFSLLSVVLVASTLFVSCNKVADVTTAPPLEERLSKDKVL
                     SDAIAAATDLYLKTSNGTLSDPNSIPALEAIAVKINNKTASTADYQAVENIVGMSYND
                     FIQELQNFGIALSKVNEKYPELAKMNQADLQATFKKSIELNPTLQNSLGASALVDGRV
                     TACPLRDICNLAVTLTRIFAGDAICAAINVTTIPVVGGLLCTLVLNLGVGLLTAICNA
                     LPC"
     gene            31423..32658
                     /locus_tag="PLL59_00500"
     CDS             31423..32658
                     /locus_tag="PLL59_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMY22308.1"
                     /translation="MNFLEVVQLAYRSVRTNIVRTIITCCIIGFGIMALVGILTSVDG
                     LKGYLSESFSSMGAGTFKIRNRGLGFNLDADADEAQKVFKSISYFEAAQFKNKFNDKY
                     PTSVQYIGAQGAVVKSGDKKTNPNILVIGSDENYVGNESYTILHGRNFTETDIKYGTK
                     VVMLGYTVAKKLFGANYTMENKVVRIDDVKFNVIAVLDEKGSSFITTDNMVLVPLSTA
                     RRLYTYPNPSFVISVKGKDPENMQPAEDDAIQVMRNVRKLHPGEEINFDILKSDSISG
                     MFVEKMEYATMAGFVIGIITLVGAAIGLMNIMLVSVTERTKEIGTLKAIGATNNTIRL
                     QFLLEAIVICQLGGLLGIVLGIAAGNAVSLSVGGTFVIPWMWILVGVVFCLIVGLVSG
                     IYPAFKASKLDPIEALRYE"
     gene            complement(32731..33177)
                     /gene="rplI"
                     /locus_tag="PLL59_00505"
     CDS             complement(32731..33177)
                     /gene="rplI"
                     /locus_tag="PLL59_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HMY22309.1"
                     /translation="MEVILLKDIENLGTKFDVVSVKPGYGRNYLIPQGFAQVANASNK
                     KHNEEIKKQQSAKIAKLIEDYKALAEKLKASKTIVGAKAGTTGKLFGSVTNIQVAEAL
                     KKQFDLTVDRKRVHIIGEVKEVGTYKATVNLYKEVSTEIEFDVVAE"
     gene            complement(33185..33451)
                     /gene="rpsR"
                     /locus_tag="PLL59_00510"
     CDS             complement(33185..33451)
                     /gene="rpsR"
                     /locus_tag="PLL59_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HMY22310.1"
                     /translation="MATQDEIKFLNSPKIGLQKKKYCRFKKSGIKYIDYKDPQFLLKF
                     INEQGKLLPRRITGNSLKFQRKISQAVKKARHLALLPYVTDLLK"
     gene            complement(33453..33806)
                     /gene="rpsF"
                     /locus_tag="PLL59_00515"
     CDS             complement(33453..33806)
                     /gene="rpsF"
                     /locus_tag="PLL59_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HMY22311.1"
                     /translation="MNNYETVFITTPVLSNDEYTRTVKKYADLIEKAGGKVVHKEDWG
                     IKQLAYPIQKKTTGFYTLLQFEVDAQFIRKFEVEFKRDENIMRFLTTRFDKHALAYSE
                     QRRNKLSGKSKKAEA"
     gene            complement(33991..34425)
                     /gene="rpiB"
                     /locus_tag="PLL59_00520"
     CDS             complement(33991..34425)
                     /gene="rpiB"
                     /locus_tag="PLL59_00520"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HMY22312.1"
                     /translation="MNLPTKIVIGSDHAGFEYKEAIKSYLAKNNVEIIDVGTNSLDSC
                     DYPDYAHAAANEIETANVAFGILICGSANGVAITANKHQKIRAALCWKNEIASLARQH
                     NDANIICLPARFITIDEAAEMVHTFLTTEFEGGRHQKRVDKI"
     gene            34517..35791
                     /locus_tag="PLL59_00525"
     CDS             34517..35791
                     /locus_tag="PLL59_00525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HMY22313.1"
                     /translation="MQTKLEARVLFIDMNSFFASCEQQTNYWLRGRPVAVCVYTGKYG
                     CVIAPSIEAKKRGVKLGIRLNEAMKICPDLVPLETNPDKYRTFHARIMKILKRYADDV
                     IPRSIDEAVMDLTSYKNIYPDVVEVAKKIKKDITEEVGDYLKCSIGIAPNAFLAKLAS
                     DIQKPDGLTIITPENIDQVLKKLELTDLPGIAKGMKLRLNKAGIYTPYDLRHSTPEKL
                     RAACKSIIGLHWYHRLHFQEVDMHTTDEYKNMSAMRMVSKEQRKEVQHLKDIMMMLCI
                     TLEKRMMKKHLFCKHVQFFIQYEEMETGYHDNFHTEQPLQDGMELFHILLDRMKVFEQ
                     KNKCSQQLLNNNIIRIGVVVQNFINDEFLQLNFFDNTHIKNNKVRKAIYEIKDKYGGK
                     TIKRASELNDEDTADDVIGFGSVKDVNESDFG"
     gene            35855..36187
                     /locus_tag="PLL59_00530"
     CDS             35855..36187
                     /locus_tag="PLL59_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HMY22314.1"
                     /translation="MGKFVIKTQKDGQFYFNLVAGNGQSILKSEAYTTKPACLNGINS
                     VKSNAADDNRYEKNESKNGKPYFNLKAGNGQVIGTSELYESVASRDNGISSVKNNAPE
                     ATIDDTTV"
     gene            complement(36238..37461)
                     /locus_tag="PLL59_00535"
     CDS             complement(36238..37461)
                     /locus_tag="PLL59_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22315.1"
                     /translation="MNTDSYIRRYLKISLLNIAIVALLGTILRYKILYSLPFLDQKHI
                     QHSHSHFAFAGWVTQTLMVLMVAYLSKQKNENKFIDYRNLLIGNLLSAYGMLLAFPIQ
                     GYGFVSITFSTLSVFVSYFFAVKYWKDLSSIKSHNTTHLWFKGALIFAVISSIGTFAL
                     AYMMSNRIAHQNWYLASIYYYLHFQYNGWFFFACAGLLQSILPAGIVHQHKKLSYIFW
                     LFASSCVPAYLLSTLWMKMPSWLYGIVIIAALMQVVAWYMFIQILLQHRQLLKDKFSS
                     HGAWVLILSAMALSIKLILQIGSTIPALGQWAFGFRSVVIAYLHLVLLGVISLFLLGF
                     IFTEKHIKVTKLSTLGLWIFVAGVFLNEFILLLQGVASIKYILVPYSNEALLATALIL
                     LSGALMLFISKFRNE"
     gene            complement(37509..38132)
                     /locus_tag="PLL59_00540"
     CDS             complement(37509..38132)
                     /locus_tag="PLL59_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY22316.1"
                     /translation="MEMIHEDILLTLGAVYKRVKKGEYIFREGAKCYYYYQLVQGKIS
                     CLNLDEDGKIFIQSIIEPGESFGELPLFDGEPYATSAIAETDCIILQLPKSAFQLLLK
                     ENTDILFSFNKLLAQRMRYKFLLLKDVAYHTPEQRILTLLHHFKQKEARHNGHADFKI
                     DFTRQQLAEMSGLRVETVIRVIKNLSKKGQLYIHRGKVFMLNNENSI"
     gene            38327..38830
                     /locus_tag="PLL59_00545"
     CDS             38327..38830
                     /locus_tag="PLL59_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMY22317.1"
                     /translation="MKKLFLIMSITAAMVIINACNSNPNKDDFSTEGTESTESTEAPA
                     TTENGNPSYDPKRGEGKFTNVEVGATLDAALASSGEKVYGVKCSSCHKLTDEKLVGPG
                     WKDVTKKRTPEWIMNFITNTDVMLDKDPEAQAQLEICLVRMPNQNLSDDEARQLYEFM
                     RKNDGVK"
     gene            38861..40852
                     /gene="nosZ"
                     /locus_tag="PLL59_00550"
     CDS             38861..40852
                     /gene="nosZ"
                     /locus_tag="PLL59_00550"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HMY22318.1"
                     /translation="MKKGIFGTLVVVAISMLTFTACKPKNAGSAVSGDAAQKVYVAPG
                     KYDEFYNFVSGGFSGQLSVYGLPSGRLLRVIPVFSVDPEKGWGYSEETKPMLNTSHGF
                     VPWDDLHHVEMSMTDGVADGKWVFVNGNNTPRLARIDLKTFRTAEILELPNSGGNHSS
                     PFGTENSEYIVAGTRFSVPADNVNGDVPISTYKQNFKGHISFVSVNKENGEMNVAFQL
                     KTPGVNFDLARAGKGKSHGWFFFSTYNTEQANTLLEVNASQRDKDFIMAVNWKKAEEY
                     IKAGKGKKQAAKYAHNKFSEETHTATSEMKNEVTVLDVAEFPDIVYFIPCPKSPHGCD
                     VDPTGEYIVGSGKLAALLPVFSFDKIQKAIADKQFEAEKYDGIPVIKYEAALYGEVKK
                     PGLGPLHTEFDANGNAYTSFFVSSEVVKWNIKDLKVIDRQPTYYSVGHLMVPGGNTKK
                     PFGKYLVAYNKITKDRYLPTGPELSQSAQLYDISGEKMKLILDYPTIGEPHYAQAVPA
                     DLISKNQVKIFKIDDNKHPYVSKGEKETRVIRNGNRVDVFMTAIRSHLAPDNIEGIKM
                     GDEVYFHVTNLEQDWDVPHGFAIKGANNGELLIMPGETVTLKWVPDRTGIFPMYCTDF
                     CSALHQEMQGYVRVSPAGSNVPLSFHIGKTEPAPAPAAK"
     gene            40932..41960
                     /locus_tag="PLL59_00555"
     CDS             40932..41960
                     /locus_tag="PLL59_00555"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HMY22319.1"
                     /translation="MDKVNEQKISNLSRILLFLSSVFLVISIFVPLWRIDLDAPQYPE
                     GLQLLIYTYKLGGNVEIINGLNHYIGMKTLHADDFIEFTVLPYIIGAFALFALITALI
                     GKKKLVYILFIAFALFGVLSMIDFYRWEYDYGHNLDPNAAIVVPGMAYQPPLIGFKQL
                     LNFGAFSIPSYGGWLFIICGVFMLAAVSIEAGWISKFKKQKPVVSILLSLLSIALLTS
                     CVSTEPQAIKLNTDNCDYCKMSIADGRFGTEVITKKGRAYKFDDISCLHGYITENKQV
                     PIGAMYVADFATPTLLTPVETAFYVKHADVKSPMGGNVAAFSDKAKAEEYAKKLNTSV
                     LDWKSLNL"
     gene            41962..43197
                     /locus_tag="PLL59_00560"
     CDS             41962..43197
                     /locus_tag="PLL59_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HMY22320.1"
                     /translation="MNRFFYIVFLIFLVHNVLGKTIVVGKGQPYTKIKTAVHVASPGD
                     SIIVLKGVYKEGNIVIDKPLSLVGKNLPILDGQKKYEVLSVKSNNVTVDGFVIQRSSV
                     ATLDDPGGIKVYNSNYVVIQNCILEDNFFGIYIQYGKHCIIRNNKIKAYGIEEQQIGN
                     GIHCWKSDSLLIIGNKVDGHRDGIYFEFVTGSTIWRNIANNNIRYGLHFMFSNDDAYI
                     CNVFKNNGAGVAVMFTKNVKMYNNYFSENWGDAAYGLLLKEISDSYIIGNHFENNTTG
                     IFMEGTSRIIAEKNSIKQNGWGMKIQASCMDNTIKKNNFIANTFDISTNGTLVLNTFD
                     NNYWDKYEGYDLNKDKIGDLPYHPLSLFSVIVEKNPPAMLLFRSFMVTLLDKSEKIIP
                     SLTPDNFIDNSPLMRPLLL"
     gene            43194..43952
                     /locus_tag="PLL59_00565"
     CDS             43194..43952
                     /locus_tag="PLL59_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMY22321.1"
                     /translation="MINIQQISKRFSKLEVLKHIDLQCNKGECIALIGPNGCGKTTLI
                     KSILGMVIPDSGEIIVNGENTKANFEYRKHIGYMPQIGRYPQNMTIGQVLQMMKEIRQ
                     TTSTLDEDLIKSFELEHLYGKAMGTLSGGTTQKVSAALAFLFNPDILILDEPTAGLDP
                     LASEILKEKIITEKNKGKLILITSHLLSELDDLITQIIFMQDGRVEFHHSVEQLRNDT
                     GETKISKAIAFILKNKIGNKDVITQSTINQSVKS"
     gene            43949..44716
                     /locus_tag="PLL59_00570"
     CDS             43949..44716
                     /locus_tag="PLL59_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMY22322.1"
                     /translation="MNRIIKYVIIDILKNKIVVAYTIMLAVLSWSAFSLEDSSTKGML
                     TLLNIILLTVPLVSILFTTIYIYNSAEFIELLLSQPIARKKIWFSLFIGLSMSFIVSF
                     IIGAGIPLLIYAPINTGVMMLLLGVIISVVFISIAFLSSITTRDKAKGIGMSIMLWLY
                     FCLLFDGIVLFLLFQFSDYPIEKIMVIITALSPVDLARILILLHLDVSAMLGYTGAIF
                     KDFFGTTLGFLISLCLLCLWFIVPYWISLARFRKKDL"
     gene            44756..45142
                     /locus_tag="PLL59_00575"
     CDS             44756..45142
                     /locus_tag="PLL59_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HMY22323.1"
                     /translation="MKKDIKNRKDIEKLVNAFYAKIKEDKLLQSFFTNPEGFNWEKHL
                     NTMYNFWENMLFFSGNYEGNPLHLHRHLHQLTNIKKSHFQRWNKLFADTVDELYAGKN
                     ADLIKKRSINISNIIQKNIIPKEMSE"
     gene            45235..46959
                     /locus_tag="PLL59_00580"
     CDS             45235..46959
                     /locus_tag="PLL59_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HMY22324.1"
                     /translation="MNGGEIIAKVLQNHGVKFLFTLCGGHISPIFVSAQKIGIRVVDT
                     RHEVNAVFAADAVSRLTGVPGVAAVTAGPGLTNTITAVKNAYMAQSPLIVLGGGAATI
                     LKGRGSLQDIDQMSLMKPVVKWAARITKVKEIAPTLERAFAVAQEGIPGPVFVECPID
                     TLYNEELVREWYGAKSKDVPPRNMQERVIQWYVNRHARLLFEDKDKIRFHEPQSFTIP
                     NHQSSDIAKAVAMLKEAKNPLMIIGSGALMQPNKADELAAAVVKLGIPVYLSGMARGL
                     LGKDANLQMRHKRKEAIKEADLILLAGVPNDFRLDYGNHIGHRPFISINRSTEDLYKN
                     KRPTIGIHADPQDFIIELAKNYAINNPEWISKLQKRDEERERNIDAQAKDKLNGINPV
                     QLFRSLDTLLSDNTILVADGGDFVATSAYTLKARSPLSWLDPGVFGTLGVGAGFALGA
                     KLVYPEKDVWIIYGDGSAGYSLMEYDTFVRHNLPVISLIGNDACWAQIARDQVDFLES
                     DCAVTLAHSNYEMIGKAFGAAGDRVETMEAFNESVLAALKNTRKGMPYIINAIIGKSE
                     FRKGSISM"
     gene            46965..47516
                     /locus_tag="PLL59_00585"
     CDS             46965..47516
                     /locus_tag="PLL59_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HMY22325.1"
                     /translation="MKNIGLINKETTALVVIDIQDKLMPVMQQAELIFANANRLLKGA
                     SILQIPSYITEQYPAGLGNTCTAIEMPSDITLVEKIAFSCMQSQQLLESWKKNNINTL
                     ILCGVESHICVLKTALDALAAGFDVHVVADAVSSRTAENKNLALERMRQSGAFIVSTE
                     MLLFQLIQEAGTPAFKAISKLIK"
     gene            47542..49314
                     /locus_tag="PLL59_00590"
     CDS             47542..49314
                     /locus_tag="PLL59_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HMY22326.1"
                     /translation="MEFKRLFDLLTNHVNENPNNLFLASKVNNKWKHYSFQEVQQVAN
                     KVSQLLLSLNLQKDDKIAVIANNRPEWNFTDFGAQQIGVTVVPMYPTISEHDYEYIFN
                     DSEIKYAFVGDNTILQKVKTLLQKCTSLKGIYTFDETEDAKSFWSALPENIDITAIEN
                     IKATVTEDDLATIIYTSGTTGNPKGVMLTHKNIVSNVISVKEVTPFVIGEKSLSFLPL
                     CHSFERMVFYAYLCFGIHIHYAESLETIADNLQEVKPYCFTTVPRLLEKVYEKIMAKG
                     AALTGAKKLLFDWSMELGAQYEIGTNKGWCYNTQLAIARKLVFSKWQEALGGNVHFII
                     TGAAAMQPRLITLFTAAGVNVLEGYGLTETSPVLSCNLVDEKQRCIGTVGIPIPGVEI
                     KMAEDGEIIAKGPNIMKGYYKRPDLTAEVIDKDGWFHTGDIGTWIEKNGNKFLKITDR
                     KKELFKTAGGKYIAPQVIENKMKESPYIEQIMVVGGDDKKYISALIVPSFVQLTAWAE
                     KNGVVVLSNEELIKDKQVLSLLDKEIKKYNDNFGQWEQIKKFVALPNEWSVESGELTP
                     TMKPKRKIIAERYSKEINSIYAAD"
     gene            complement(49379..49942)
                     /locus_tag="PLL59_00595"
     CDS             complement(49379..49942)
                     /locus_tag="PLL59_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HMY22327.1"
                     /translation="MKNYLLMLFAVTMLLFTACKNNEAAGDASAASEESSPAVGQSGV
                     QDDVSNPNIVQVAAGSKDHSTLVAAVKAAELVDALSNAGPFTVFAPTNAAFEKLPKGT
                     VEELLKPEKKEDLKNILEYHTYVGSLKAEYFQDGQSFEQVNAQKVTISVKDGKTYVNG
                     TAEIVASIPTSNGMIHVVNEVLLPPTK"
     gene            50348..50680
                     /locus_tag="PLL59_00600"
     CDS             50348..50680
                     /locus_tag="PLL59_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HMY22328.1"
                     /translation="MIVRLIKKKTIEDFALQNANSRSSFKLWLSIIKLADWETPDDIT
                     ATFGSADLLGNGSNRVVFDIGGNNFRMICKYHFSKTLVHLYIKWIGSHSEYDKLCDKN
                     KQYTIDIF"
     gene            50690..51103
                     /locus_tag="PLL59_00605"
     CDS             50690..51103
                     /locus_tag="PLL59_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMY22329.1"
                     /translation="MSTLQYKIIKTETQYKKYCDILESLVDSGKNTKAVQDEIELLSL
                     LIEKYDEEHNSFDDLDPIQLLKGLMKEHKMKAVDLSNLLNVSDGLVSDILNYKKGLSK
                     ETIRVLADRFKLNQKAFNRPYSLKIATTKKELAFA"
     gene            complement(51241..51315)
                     /locus_tag="PLL59_00610"
     tRNA            complement(51241..51315)
                     /locus_tag="PLL59_00610"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51278..51280),aa:Pro,seq:tgg)
     gene            complement(51336..51422)
                     /locus_tag="PLL59_00615"
     tRNA            complement(51336..51422)
                     /locus_tag="PLL59_00615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51386..51388),aa:Ser,seq:gct)
     gene            complement(51589..53025)
                     /gene="dnaA"
                     /locus_tag="PLL59_00620"
     CDS             complement(51589..53025)
                     /gene="dnaA"
                     /locus_tag="PLL59_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HMY22330.1"
                     /translation="MKNKHELVWENCLKIIKDNINVQSFKTWFEPIKPVKLDANSLTI
                     QVPSQFFYEWLEEHYVTLLRKTIKRELGDDAKLEYRILMDTSNGTKTAPKTIDVPNHR
                     EGVYGTNEVSMPTIFSSPMVKNPFVIPGLKKIHVDSQLNPNYTFDSFIEGDCNKFARS
                     AGMAVAKSPGGTAFNPLVIYGGVGLGKTHLAQAIGNYIKVNNSTKTVLYVSSEKFTSQ
                     FLNAIKDNNTNDFIHFYQQIDILIIDDIQFFANKTKTQDIFFHIFNHLHQNGKQLILT
                     SDRAPKDLENMEERLLSRFKWGLTTDLQTPDYDTRISILQKKLHADGIEMNEDVTRFI
                     ATNIKTNIRELEGVLTSILAQATFNRQDITLDLAKKTVINFVKHATKEISLDMIQKTV
                     CDFFEVPVEKLKEPTRKRQFVQARQLSMFFAKEYTKSSLKAIGHQFGGRDHSTVIHSC
                     QAVRNLIDTDEEFRDSVEELKKRIELSL"
     gene            complement(53107..54042)
                     /locus_tag="PLL59_00625"
     CDS             complement(53107..54042)
                     /locus_tag="PLL59_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMY22331.1"
                     /translation="MQNRRTFIKQSVLSGAMLTAVGSPLSLLADNTKSALTVLHTNDV
                     HSHIEPFADDDKKYPGLGGAAQRAAIVHEIRKNKEHVLLLDAGDIFQGTPYFNFFNGD
                     IEIDLMNKMGYDAATIGNHDFDGGIDGLAKQITRANFPFVNCNYDFTGTPVHGKVHKH
                     IIIKKGKLKIGITGCGVELDGLVPDKLYTSTKYLDPISCVQEQVDILKHNEKCHLIIC
                     LSHLGYQYQSTKISDIILAQQTNHIDVIIGGHTHTFLDAPVSQKNKDGKTIYINQVGW
                     AGIQIGQLDYLFEKKFNKKLSNTQTVIVDKKTRGK"
     gene            complement(54032..54748)
                     /locus_tag="PLL59_00630"
     CDS             complement(54032..54748)
                     /locus_tag="PLL59_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HMY22332.1"
                     /translation="MLAIVVFTACKTYVPIQEKFSLNKINKTIIADSATLAYYLPYKD
                     SLDKIMKIPLAELAEDLSKKMPESTLGNMMADILKLKTAAYTNQTIDVAILNYGGIRT
                     PSLSKGTLMVEHAYLLMPFDNYLVEQILTGQQLSDYCDSLAIKGGWPVAGIAFVIKNK
                     KASDIRVNGIPIDLKKNYNVATNDYLANGGDGMTFLKTIPQIQTGKLYRDAIIEYWKE
                     QTKAGKKIQSNLENRIQYAE"
     gene            complement(54765..55304)
                     /locus_tag="PLL59_00635"
     CDS             complement(54765..55304)
                     /locus_tag="PLL59_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22333.1"
                     /translation="MLTTILEILKYTVPSLVVFGGVYSIIKVFIENENKRRNQEINKS
                     LLFEQRKVSLPLRLQAYERILVLLERIHPFSLLTRVYQPNMLVAELQLELIKSIRIEY
                     EYNLSQQMYINDDAWQMVSTAKEETIKVINLFASKLPDDADGIELNKAIFNYYMQSDK
                     DVPVQIAINYVKSDVKKLF"
     gene            55465..55812
                     /locus_tag="PLL59_00640"
     CDS             55465..55812
                     /locus_tag="PLL59_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMY22334.1"
                     /translation="MIDTVNKLPISLTQNAVQEILHLYKNGNYEAGFGLRIGVKGGGC
                     AGFSYVLEFDAQKENDNVYSINSIPLYVDKSQELYLLDCEIDFKGGLDNRGFIFNNPN
                     ATSTCGCGTSFSA"
     gene            55955..56236
                     /locus_tag="PLL59_00645"
     CDS             55955..56236
                     /locus_tag="PLL59_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMY22335.1"
                     /translation="MNKGDLVKVIADAAGVTNAAANDTLNAVLDSIRKTLKKGDKVTL
                     IGFGTFSTSKREKREGRNPATGAKIKIPAKTIVRFKAGKELSEAVKKNK"
     gene            complement(56308..56949)
                     /gene="pdxH"
                     /locus_tag="PLL59_00650"
     CDS             complement(56308..56949)
                     /gene="pdxH"
                     /locus_tag="PLL59_00650"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HMY22336.1"
                     /translation="MPQRDLNNMREQYDADILLEANCHTNPFQQFDAWFQDAIQQNII
                     EPNAMTLATVGHDGQPSARIVLLKSFDKNGFVFYSNYESHKGKQIQENEKVALLFWWQ
                     QRQIRIEGAVQKLDRESAMQYFHSRPIESQIGATVSLQSSIIESREILDEKYDTAYKA
                     YANKVVPLPDYWGGYLVVPTLFEFWQGRMSRLHDRIQYSLEANQDWKIERLSP"
     gene            57003..57707
                     /locus_tag="PLL59_00655"
     CDS             57003..57707
                     /locus_tag="PLL59_00655"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HMY22337.1"
                     /translation="MKNNNIDNQASQNFKRAALPLIIFTNLFPIYGVITYNWTIFSIV
                     YLYWLELLIVSTFQLLKILTAEGDKEITMIGKLFLAIRFFLFRTGIFLFYLLFIVTFL
                     GLLITNKENSDSELLTFAHALTLKGSFYRITLTSFFIYNLVEYLVQFRLNGKYKTSTP
                     NDNFTLLDPHIIVVHIVVVLGTFLYAGVTEKLSLDHHTAIIACAILFAIVKIIADVVR
                     YQMSDKSATISSEKYI"
     gene            complement(57710..58414)
                     /locus_tag="PLL59_00660"
     CDS             complement(57710..58414)
                     /locus_tag="PLL59_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMY22338.1"
                     /translation="MKLHFIALLLTISFISTSCNSQNAGQVLTPTAFNEKINTTSDAE
                     ILDVRTPEEYAGGFIKNAQNIDYNSAAFEEEISKLDKNKTYFVYCLSGKRSASAASVM
                     RKNSFANVVNLDGGILAWQSANLPIVTENSKAAADKITMEMYQQMITSDTLVLVDYFA
                     PWCAPCMKMKPMLEEIEKEYAGKVKVIRLDINENKQLAQQLKIAEIPILKTFVKGKET
                     WVHKGYIEKADLVKNL"
     gene            complement(58418..>58690)
                     /locus_tag="PLL59_00665"
     CDS             complement(58418..>58690)
                     /locus_tag="PLL59_00665"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMY22339.1"
                     /translation="NSCLMTTFLAIAENSKLEYLSFDSKAIGKLEMIDGKYMMSEVEL
                     QPEITIANEQDKEKAERVLIKSERACLISNSIKSTIVFNPTITIKQ"
BASE COUNT        19451 a         9786 c        10584 g        18869 t
ORIGIN      
        1 actacacctt ttccaaattc taaaaaagta tatatacaag gtgtgcaaca cgatatacaa
       61 gtggcaatgc gcgaaattac actgagcgat accaaattaa acggtaaaac attacataaa
      121 aatccagcag taactgtata tgatacttct ggagtttata cagatgaaaa ctatacggta
      181 gatgtacgca aaggcattcc gcgcattcgc gaaaaatgga ttttggagcg tggcgacatc
      241 gaacgtttgt atgaattttc ttctgattat ggcacacaac gtatgaacga cgaagcctta
      301 aatcacttgc gtttcgagca tatcaaaatg ccattacgtg ctaaagaagg aagaaatgta
      361 acacaattac attatgcgaa aatgggcatc attactccag aaatggaata cattgccatt
      421 cgagaaaatc aacgcattgc agaatataat gcatcattaa atggacaagc agcggttttg
      481 tgtcaacaac acgcaggcga aagttttggt gcaaacacac caaaatcatt tatcacacca
      541 gagtttgtgc gaaatgaagt ggcggaaggt cgtgccatta ttccttgtaa tatcaatcat
      601 ccagaaacgg agccgatgat tatcggtaga aatttcttgg tgaaaataaa tgcgaacatt
      661 ggcaatagtg cagttacatc aagtatcgaa gaagaagtag aaaaagcagt gtgggcttgt
      721 cgttggggcg ccgataccat tatggatttg tctaccggaa aaaatataca cgaaacgcgc
      781 gaatggataa ttcgcaattc gccagtgccc attggcactg taccaattta tcaagctttg
      841 gaaaaagtaa atggaaaagc cgaagatttg acttgggaaa ttttcagaga tacattgata
      901 gaacaagccg agcaaggtgt agattatttt accattcacg caggtgtctt gttgcgctat
      961 gttccattaa cagcaaaacg tgtaacaggc attgtgtccc gtggtggtag cattatggct
     1021 aaatggtgtt tggcgcatca taaagaaagt tttttatata cgcactttga agaaatttgc
     1081 gaaatcatga aacgatatga tgtagcgttt tctttaggtg atggcttgcg cccaggttct
     1141 attgcagatg cgaatgatgc tgcgcagttt gcagaattgg aaacattggg tgagcttaca
     1201 aaaattgcgt ggaaacacga tgtgcaagtg atgatagaag gaccaggtca tgtgccgatg
     1261 catttgataa aagaaaacat ggacaagcaa ttgaaggaat gccaggaagc accattttac
     1321 acgcttggac ctttgacgac agatattgcg ccaggttatg accatattac atcagccatt
     1381 ggcgcagcca tgattggttg gtatggttgt gcgatgctgt gttatgttac gccaaaagaa
     1441 catttaggat tgcccaataa agaagatgtg cgtgttggcg taattaccta caaattagca
     1501 gcacatgctg ccgatttggc gaaagggcat ccaggtgcac aatatagaga taatgccttg
     1561 agtaaagcac gtttcgaatt ccgttggcaa gaccaattta atctgagttt agatcctgaa
     1621 aaagcattag agtttcacga tgaaactttg cctgctgaag gcgcgaaaat agctcatttt
     1681 tgttcgatgt gcgggccaca gttttgcagt atgaagatat cacaagaagt aagagaatat
     1741 gctgaaaaag gcatgcaaga aaaatctgaa gaatttatag aaaaaggaag cgaagtgtat
     1801 ttgtaatgta ataatgaaat aatgtagcaa taaggtaatg aaataattag aaaaaaatta
     1861 accttctata ttgcaacaga tgttaaagga ttttttaaaa atgatgtaac tattgataga
     1921 acagatttat aatttcaata atataaatag atttttttgt ataatatttc tcaaaaaatg
     1981 cctaatttag aattgtgaaa tccaaaacaa ataataacaa acaagaagtg cgcaaactca
     2041 caccgcatga tttggcattt ggtattggaa gacctgcaac aattgaagaa ttagatttgc
     2101 taatggaaga tatggaaaat gaaacattta cagaagcaac aactgtaatg aaaagaattt
     2161 ctaaaaacct gcaaaagaaa aatcaagtaa aaagtgttaa aggtaaagta tagtaaaagt
     2221 gctgaaaaaa caattgaagg aatcacggat tttgttgaac aattaaatac caaaggtgcc
     2281 ggtgagcgtt ggaaagaaag gtttaataga aaaattcata agtatgctca accaatttct
     2341 tatgcattgt gtaggcatct tgattttgct ttgaaaaaat atagttgtgt ctcaatagaa
     2401 aattgggtaa tcatatttaa ggtaaagaat gacgtgtttt gtgtgtatgg cattattcat
     2461 tcttcgttat taaaataaca ggattgcatg caagtaatca tctacacatc accaacagct
     2521 attgaaaatg aaatagaaat tatttctcaa ttatttgatt gtggtctaga ttatttatat
     2581 ataagaaaac cagacttaga tgatttttct ttggttgatt ttgtagagca aattccagaa
     2641 aagtattgga aacgttgtat ctctacttcc atgataatta ctaaagaatt taacttaggt
     2701 ggctatcact ttacgagaga tattgtccaa aaaaacgaaa aatacaacga taaaattctg
     2761 gattggttgc actcaaatca taaaataagt tctgtctcgg cgcataagct cgaagaaata
     2821 gagaaatata aaataaaatt taatcatgta attgtatcac ctttatttaa tagcatttcc
     2881 aaagaaaatc atcgttatga ctggaatttt gctgaattaa aaaatgtact tcaaaatctg
     2941 aaatctgaaa acataaatca taaaatattt gcagttggtg gcattgattt ggacaagata
     3001 gaaatcgtaa aaaatcttaa ctttgatggt gttggtttgt taggtgccat ttggtcaaat
     3061 accgataatg caatagaaaa atttagtaca ataaaaaaac aatacgaata aaaatgagtg
     3121 cgataaaaaa aagaccatac gtattgtcga ttgctggcta tgatgcttgt gcaggcgcag
     3181 gcgtattggc cgatgtgaaa acattcgaga atattggtgt ttatggcttg gcagtaagta
     3241 cttgtattac ctatcagaat gacacaaaat ttgaaggcgt acattggttg tctaaaaaga
     3301 aaattaaaca acaattgtat cctattttag atgcctatac aatcgattat gtgaaaattg
     3361 gattgattga aaatctaata gtactaggtg atgtcattca gttattattg tcctacaatc
     3421 caaaaataaa aattatttgg gatcctattt tacgagcaag tgcaggattt gattttcata
     3481 aaaaattacc taaaaaaata agcaaacata ttttctctgc tatcgattgg ataacaccaa
     3541 attggaacga aataagttta ttgtcgagtg aaaaagatgc cattgtcggt gctgaaaaat
     3601 tagctatatt ttgtaatgtc tatctcaaag gcggacacaa catcgaaaca ccagcaacag
     3661 atatggtttg gataaataag aagctggata ttttgcatcc aaaagaaatt acagcattaa
     3721 gcaaacacgg aacaggctgc gtactttcat ctgccttaac tgcctattta gcattgggaa
     3781 atacagcgca taaatcgtgt gagttgtcaa aagaatatac gtatcaattt ttaatcagca
     3841 ataattcaaa tttaggctat cataagtaaa tgcatatttc aagatttcat tatttaacgc
     3901 aagatttgcc gaatatttct caccaagact tggctgaaat cgcctgtaaa aacggcataa
     3961 agtggctgca attgcgtgta aaaaataaat ctcttgatga atatttattt atcgcaaaag
     4021 aggtaaaaag aattacggat aagtataaaa gcactttaat catcaatgat aatgtagaaa
     4081 tttgtaaaga gataaatgct gatggtgtac atttaggaaa gaatgattta gcacctttag
     4141 aagcaagaaa aatcttagga agtaataaga ttattggagc aactgcaaac acagtaattg
     4201 acattctgca acatcaaaat gatggtgcta actatattgg cttaggtcca tataaattta
     4261 ccaatacaaa gaaaaattta tcgccagtat tgggtgttga agcttataca gaaatattta
     4321 aaaataataa tttccaaatt ccaattgtcg caatcggtgg tattcaatta gcagacatga
     4381 aaatactaat gaatacaggt gtttacggca ttgctgtttc ttctgccatc aatttgtcat
     4441 accaaaaaga aaaaactatt gctgactttt tgcagcttgt ttaaccaaca aatacatatc
     4501 gcaatcgcta aaaatttctt tcttaaaacc tgcttgttca tagttagtaa tgaagttttt
     4561 ccaagtgcta tctgatgaaa tatgcggatc tcttaataat gaaggcaaga caatcaccat
     4621 atctattttc gcggagtcaa cataatgtgt aaacggcatg tgttttttct tgtcaaaaat
     4681 ggtgtttatt tccttaaaat tattcggtaa cataccatgt acaaatggta aattggtgaa
     4741 gatagtatga ggtttgctgc tgtatttatt ttctaaaaaa ttaactagtt ttttattgca
     4801 taatgtttgc ttgtcgccat taaattgcat aaacgaaaat gattgaatac tcggcgttgc
     4861 aaggaataac aatacaccaa aaatagtaaa ggatagcttg tttgctttta ttttattaaa
     4921 tggatatgcg gcaatacaga ctaaaatgat agtgtaaaat ggcatttgca aatagaggta
     4981 atgatcgcgt ggaaacacag ccaaacaaga catgagcgta ggtgtgataa agcaaaataa
     5041 taaaagcaaa tagaattgat aggcttttat tttttcgata aatatgactc ttgatttttt
     5101 tctaaaaagt aaaacaacaa tagtaaacaa tacgataata gtcaaagcca attgcttttt
     5161 cttgccataa aataagccaa ttggaaaaat aaatgagcat acttttagta attgtaacaa
     5221 ataagttctt acattaaata gcgtattgct gataaaaata ccaggttgag ttttgataac
     5281 acataaaatt gaattgcaat cgccaaattg tttttttgct aagtcttccc aatttaacca
     5341 agcatcgaaa gtagcgccag tgcgcatttt ataattccat gcataatgct gatagaatgc
     5401 ggcaacgcca cgattataac caaaaaaatc attggaagga aatcggaata taagatgtaa
     5461 aagtccaatg attaaaagca gaaacgaaag aatataaaaa tcatttttgc tcagaaattg
     5521 cttgttgaca tataaagtag ctaggctcac taagagcatt atcataaatg aaagataaaa
     5581 ttctggacgt gcgtaggcac ataataaaca agctattgtg aatgcaatac atttatagta
     5641 aacaggcttt atgtagctgg aaataattaa gctaatcaaa catatcatta aacaaaaatg
     5701 agacacacgc ggccatacag atacgttcaa atcactgact aatatgcaga atgaaatgaa
     5761 caaggcaaca attggtgtta agtggagacg tattaaaaat ataaacatca ataggctcat
     5821 cgctatcgat gtgattgcaa aattgatata atacaatagt atcggcgatg tagtaaaata
     5881 agacaaacac ttataccaaa cggcgtaaag cggtccccaa tttctattca ttttttcaaa
     5941 taatgctaat ccaaattgaa tgtatgccgc ttcgtcggcg aattgaatat ccatgtattg
     6001 ctgcatgtga tagtacaatt tgatgccagc agccaataaa ataaaaatag aaaaggctat
     6061 ttgatatcta tttttttcaa taggtttttc ttgtgttgta gtcaataatt gaagtatatg
     6121 ttgtttgatg taaaacacaa aaagagattt tatttttttt gtaaaatata aatggcacat
     6181 tcattataca cttgtttttt ttcaaaacca aagttttcgt agtgtaacaa aaattgttgc
     6241 caggtactgt cttttttcag atatggattt ttcaaaatat tttcattcac caatacaata
     6301 tcgatgttaa attgttttct gatatggtcg aactgcattc catctttaaa atcatattct
     6361 acactgtaat cgctataatt gtctggtagc atataggaaa gattgagaat gtcagaaaaa
     6421 ataatatgct tggtactgtc atctttattg aagtgttgta tgtatttttg tccgcacata
     6481 tttcctaaat catgattatc tctaaaaaag cgatattcgt ctgcctttgg agaaatcaag
     6541 aataatacta tgaatacagg cactatagaa agtattggca attttatttt attggcaatt
     6601 tgcgttaaga aaaatgcgag tatatatatc agcagaaata ggtgacataa gatataatgc
     6661 attctcggaa aaattaaaac acttgtcatc atgctgggca atccgataat agcaattgcg
     6721 ataaattgcc atttataatt ttttatacta ttccaaatgg caatacgaaa tggcttatat
     6781 aatatgccac caatgatgat gatccaaatg aacacacaaa tacctatgaa aattttatgt
     6841 ttgccaatca acctaattgg aaataacaag ctgcctaaaa cgctagtgat gcttaataaa
     6901 taattgggaa tattgtaagt gatatttttt aaaacagcta aaggatacgt ttttacgatg
     6961 caggaaaaag agtcgcacaa aggaaaaact ttattggcat atacgcgcca accagccatg
     7021 gcatctacgg ttttgtcgtg gtgcaagaat tcatttactg ccacgtgttg gcaaaaagcg
     7081 atgtacattc tgtccacatt tcctctgtaa aaagaggcag gcgaaccaaa tacgagttga
     7141 aagaaaatca ataatatgat ggcaataaac accattattc gtatttggct aaatgctgtt
     7201 ttgtttttat accaaacgaa taatgcaagc gcatatacag caaaagaaaa tggtcgaatt
     7261 tcaggtcgtg cataagcggc aacaatagaa atagcagcga ataaaaacaa tttggtggtg
     7321 ccctctttta agaaagagga aatgtataaa ccaattaaaa tgcaaatgaa cacgaaatgc
     7381 gaaactcgcg gccaagtttc gatattgatt tctgatagga aaaataaact tgatatcgta
     7441 aaggcaacga ctaaattgat attgtagcgc accaatacga caaataagag tattgtagct
     7501 agtatggcag tcagcttgta attaagatag tataattcta tttttgaatc ggcgaagtag
     7561 gagagtgctt tgtaccaaat gttatagctt ggtccccaat ctttgtgtat cgtgtcaaat
     7621 aatgcagtgc cgttgcgcag atattcggcc tcgtcagcag ctaaaatatc catcactgct
     7681 gctaaattgg cttgccattt atagcctaag aaaagcaaaa ataagatgca taaaacatag
     7741 gtacacttat acaatacgct ttgtttgtcc tcgttgattt ggcttttcca tgaatgcatc
     7801 taaaaagaaa atttagcttt gaaattggct tttctctacg ataaagatac gcttaattac
     7861 ttgttttctt ttttaaatat ttttctatcc atcaatttat gaataggtcg ttcaaagaat
     7921 ttgaaaatga tgcctgcaaa gaaataatcc attaccaaaa atgaaaataa tccaataaca
     7981 tctgcgatga ctccaggtaa aaacttacgc aaaatacctg caacgagaaa catgccaatt
     8041 agaataaaca agaattggaa aatatacata cttaatgaca atgcaccggt acgcatgagt
     8101 actggatgca agagtagctt aaatttctcc ggaaaattta tgatgcaggc aaacaagatg
     8161 aaaataaaaa atgcaacggt agagtcgatt acttgaatgc cataaaatgg aatcacataa
     8221 atatcttccc ttatagtagt gcgtaataat tctccggtgt ctatgctaat taaataagct
     8281 ctgtaaaatg cccagccata gcacaagacg gctgcaaaaa taagtgcaat aaatagtttt
     8341 tttgattttt taaaatcata caagcaaagt ataaaaccat aaaaaacggt gtctaattgc
     8401 attaaaggaa ttttttcaaa aatgtattct cggctcaagt cgtcgccagg aatgctaagt
     8461 aaatattgcc atgcaataaa tcggaaaatg accatggcga ttgctccagc cactgaaatc
     8521 tttaacaata atgctctgtc gcgaacgaag gcaataaata aaaagatgat gatgcaaagt
     8581 tgtaagtcaa atgagatata ccaaagatgc ggataggttg ttggcgattc tggaacatat
     8641 ttatgcaata caaaaagatt ataaacttct ctaaagttgt acgtgtaggt aaataatcca
     8701 atgccaaatt gttttaattc tctgaatgta tctacttgtg cagcttctaa cttgaaaacc
     8761 agcattaata ccaatgcgat gagcagcata acaaaaatgt acagataata gactggtaaa
     8821 attttcaaga ttctttttag cacaaagtct ttaaatgttt tccatgcgtt tatgttttta
     8881 tcttcaaatt ttttaaacaa gccaacactg acaaaatatg ccgacaacaa aaagaatgaa
     8941 tgcgtacctg tataacctaa tacgcttgga agattatatt ttttaagtcg ataatgtatc
     9001 ataaaaatta aaatggcagc cacaccgcgc cagccagcca aataatacat cggattgctt
     9061 ttgtctattt tttgatcttc agccatgtgt tgattttact cgttataaat cgaatttgaa
     9121 tgacaaacaa atgaaattac tttttaaatt tagctaaata tttatctttt ttctttaaaa
     9181 aataagtttc catagtattg taactaaagt gagaaagtaa taatatgcct gaaatagata
     9241 gaatcattga aaagagttcg gcaaggaaac ggcctacttt caaactttca aaaaaatagg
     9301 gaaaaatata tacaaacact ggcaaaataa atatgtattg atagacatac gagctatagg
     9361 taattcggct tccaatatca accaaatatt tgtttctgaa aaagctaaat ctattctcac
     9421 ctttaaagca aatgtagaag aaaacaacgc acaatatatt gaccaaacaa tcaatgtata
     9481 tatattgacc atttctcgtc ataaagtcgt agctcgttat gatatgcata aaactttcgc
     9541 cagtttgctg gtgtattatc cagccattta ctggaatggt aagtatgaaa atactgagca
     9601 cagcatagaa aattctgttc atggttttat cgctccattt gatatcgaaa atagctaaga
     9661 aggtgccata aaaaaatgaa tcgtactgaa aaatcgtagc acgatataag ataaacgaac
     9721 gatgcatatt gtcatcagtt aattcacata gccagttaaa tccgacaata cgaatgatag
     9781 gaaaaataat aatcatcgca atcgttaagc gtttgatggc tttttcactc ataaaatata
     9841 tcatgaatgg aacgataata taaaactgct cttctaaaga caaagaccat aagtgtggaa
     9901 ataccatgca tttcgcatac atcaaatctg taaataagca atataaatct ttaaaattat
     9961 aagtgaacgt cagcaacatc caaccaaaat atttcaactc ataaatgata cctaaaattg
    10021 attttgctgc cgtcggatag gtaagtacgc ctacaagaac agtaaataat aaataagcga
    10081 agtaaacagg gaaaatacgt aatgttcttt tgatataaaa tttaaagaaa aatgttttaa
    10141 agctatctga acgttgcttg tctatcatta atgtgcgagt tattagaaat gatgacatga
    10201 taaaaaaact atgcatcgta aaataaccaa tatttgatgg taagtttaga taggtaaaat
    10261 gatggtggat tattagaata gtaaagcaaa atgaacctct gaatccatca aattgtggta
    10321 agtatatgtt ttttaaccta taatcgttat cagccatatt tacaaataaa ttctaagatt
    10381 gctaatatag attaaataat tgaatgccac tataatcaga tatacgtaaa aagtattttt
    10441 aaattatctt tgcatctgtt tatgcaaaaa gccatcgtca ccggagcagc cggacatatc
    10501 ggatttcatg tcaccaaagt actcttgtct aaaagctatg acgttcattt gttggttcgt
    10561 agcatcaata gcaatattgt tgaattagaa gccttaggcg ctaaagtcta tccgtgcaac
    10621 ttatttgaaa cgcaaagtta tgccgctttg ataaaagatg ccgatgtgct ctttcattta
    10681 gcggcagaaa acacgacgtc gatgtccaat gcatctcgtg tattagaaaa tacagataaa
    10741 cttacacaag tagtattgtc tgcatgtcat gaaaatggtg ttaaaacggt cgtctatacc
    10801 agctcagttg tagtaatagg tcgtagtccc aatgcacatc aattattaac ggaaaaagat
    10861 gttaatgcat tcccagaaag cccgtatgta ttaggtaaaa tgcaagccga aaaatatgtg
    10921 gtagatttta tacaaaaaca taattacgat attcgcagag tgtatccatc ttgggctgtt
    10981 ggtcccaatg atccaagact tacgccgcca cataaagtga taaaagattt tttagaaaaa
    11041 ggtgcacccg tatattttga tggtggtgtt tccattgcgg cggttgagga aattgcgaaa
    11101 ggccatgtgg cagcatatga aaaaggaagt tttggtgaaa cctatatttt gtctggcgag
    11161 aacatcacct tcaaaaaact gtacgaatta ttggtggcga atagtcgcca aaaaatgcca
    11221 ttattgaaac tgccgaagtt tgctattgta ttggcagcat ggatttttac aaaactattt
    11281 aaactttttg ggaaagaatt tccaatcagt ccggaatatg caaaagccgt tgcaggcagt
    11341 tactcttggt atgattgcaa taaagcaaaa tccttattag gttatcaaac acgacctgta
    11401 gaagagattt taaaaaatgc agtacatgat gcgaatcgga aaattctagg cgtacatcta
    11461 ttgggcaaaa aaacgcactt agctaactca acgcatataa cacaatcaga cgatattaat
    11521 actaatgctg ccggaacact tttaattact ggtgttccag gttggttggg caatcgcatg
    11581 attgatattt taattaatgg cgatagattt ggacaacatc acagcaacag gaaagtgcga
    11641 gtgctcttag aaccacgatt taaaggcttg ttgcatttgc ctaataacta tgaaatagta
    11701 tatgctgata ttacgaataa agagcaagtt catgcagctg taaaaggtgt gcattcggta
    11761 tttcatttag caggtgctat ttatccaaaa aacattgatg ttttatataa agtaaatgaa
    11821 gaaggcacaa agcatttagt agatgcgtgt atcgcaaata aagtacgccg ttttgtgttt
    11881 atgtccaccg actcgacatg tggtcatggc acaaaagacc aagctatttt tgatgaaaat
    11941 actattgcta cgccgtataa aaattatgga caaagcaaat acaatgcaga aaagtatatt
    12001 ctagaaaaaa ccaaagaagg caagattgat ggcacctcat tgcgtggttt ttggtttttt
    12061 ggtccatttg cgccagctcg acagcttact ttcgtcaata tgtttcattg gccacgacaa
    12121 attgtgtttg gcaatggcaa aaatttgcgt agcatttcgc ataccgataa tatcatcgag
    12181 gcattttttc aagtagaaaa caatccagct acttatggca gttggtattg gataggcaac
    12241 gaccagcctc atcctacagt aaatgaaatt tatgcaagca ttgcaaaggc attaggtgtg
    12301 ccatataaac cattttatat tccgaaattt ttttgcagat gttttgaaat tgccgatact
    12361 tttttaggta agtttaatta cttgcatcca acaatacatg ccgccggtaa gtttgattat
    12421 gatattgctg gcaatattga taaagcaaag cgcgactttg gatatgcgcc aaaagtaagc
    12481 ctcgatgatg cggcaaaaga gttgaaagat atgacgaatt aagctggttt ataaagtaat
    12541 attttataat gtaacaagct ttaattagta atgtgaccta atttgatagc tcataaattt
    12601 tacacatttt tctttattgg tgtattacaa atagataata tgtatcacag caaacaatat
    12661 atttatttca ttttgcaatt tgccatctta gatttacttt tacagcatgc aatcgcaact
    12721 tcaaatagct gataaattat tctcttcaag attatttcta ggaaccggaa agtttggctc
    12781 gccgacattg atgcacgatg cagtactttc ttcggaaaca caattggtga cgatggcttt
    12841 aaagcgcgtg gatttaaaaa cacaatcgga taatatcttg acttacttgc gccaaacgaa
    12901 agtacatcta ttgcccaata cgagtggtgt acgtactgcc aaagaggctg tgtttgcagc
    12961 acaagcagca cgagaagcat tggaaacaaa ttggctaaaa ttagaaattc atccagaccc
    13021 acgttactta ttaccagatg ctgtggaaac actaaaagcc acggaagagc ttgtaaaatt
    13081 aggctttgtg gttttgccat atattcatgc agatcctgtt ttgtgtaaaa gattagaaga
    13141 agtaggtgca ccagcagtaa tgccattagc cgcaccaata ggaagcaata aaggattgga
    13201 gactaaagaa tttttacgta tcatcatcga gcaaagcaaa gtgccagtta tagttgatgc
    13261 aggcattggc gctccatcac atgccgcgca tgctatggaa ttaggtgcag atgcggtttt
    13321 ggtgaatacc gcaatagcca ttgcagaaaa tccagtgcag atggcaatcg cctttaaaat
    13381 ggctgtggag gctggtcgtt tggcatatga agccaagttg gcaaaagtgt cagatagagc
    13441 ccatgcgagc agcccaatat catcattgct ggaagattga tataaggaga ataattttcc
    13501 aaaaaattat tttagattac atttcaactt aattttttta aaaacatttt tctagatgaa
    13561 tacttattaa ctacaccatt ataagattag taattagaat aaaaaaaagg acaatggaaa
    13621 atcaccattg tccttttata aataaaatca gttattattt ttttataatc ttgtgctgat
    13681 gttgatttcc attgttatcg atataagata caacataagc gcctgcactc atttcttgga
    13741 aagaaagttc aatttcgttt aatccttttt gaacagaaat aggcttaata ctgatgtgtt
    13801 gacctagtac atttgagatt tgtatatctg ttgtgtggtt ttctgtagct tgtataataa
    13861 cagttacatt atcagagaca ggatttggaa atacagaaac gataccgtct gatactactt
    13921 catcaacagt aatatttatt acattgctat attcgaatgt tgcatcattg tctatcattt
    13981 ttagtctgta ataattattg cctgtgatag gatgattatc ttggaaataa tagttcgatt
    14041 gcccattgct attgcccatt gcaacttgtt ctccaatgta ataccaattt ataccatcat
    14101 ttgatttctc tacttcaaaa cgatttgcat ttctttcaga tgctgttttc catgataaat
    14161 tatttttcaa atcagcatag acaccagaga aggaaataag ttcaactggt aatgctgcaa
    14221 atgggaatcg attcggatgt aagtaaaaag tagaaaaact atttaacgta aatgttgctg
    14281 cgtaatcagg acctgttgtt gtatttggat atcgtgttat acttggaaaa attaattttg
    14341 ctgaactatt tgttgccgga tagtttggtg gatttgtaaa tgtgccattt gaaccattgt
    14401 catatttagt aatgaccaat tcatttttat cagcaaactc atacgctgtt cctgatgctt
    14461 ttaattttaa tgcatcgtat tcagcttgtg taaagaataa gcttacaata acatcttgcc
    14521 ctgtttttgt gttcgggctg atagaccaat acctagttaa ataaggctgt agatcgccga
    14581 agtttgtcaa aacagatttc acatttgtaa ctaatggact tgttgagttt gcattataat
    14641 catagcctaa gcagagttca gtacttccca tttcaatact tccaccagtt ggatctatta
    14701 ctttcgccat tattttacca gcattgtcat aatacgaata cgagttacca ttttttacgg
    14761 tacaagtttt acaattataa tattgaggtg tgctcgtcga ttgtgtcgat acggtattaa
    14821 aatctaaagc tgtaggcgct attgagatag gcgtagctaa tgaagtagaa caagcaggtg
    14881 tattgtcagt gtaaaacaat gtccactgaa ctagtgttgt atttcccgtt gaagacaaac
    14941 ctgtgatatt tgtagatgga tttgtagcat cattaaaagt gccatcgcca gaaacaattg
    15001 tccaaattcc agaaacatca gtaataggtg gtgttgcgat tagttcagcg tatggttgat
    15061 tcggattcgc acaaggtgga ataacagccg cattggcaga tggattaagc tgtggtcgta
    15121 ttgtaatact aactttatct gtccatgcat aacacgtgga tgattttgtt cttctttgga
    15181 aacaatacgt acctaccgta gttgtattgc caactatatt tattccggaa agtaattgtg
    15241 cggcgctaat attatttgat gttgctccac ttattacatt ccaatttgca tttggtgttg
    15301 atgaagtacc tgttgttgga ttattgcatg ctgtacttgt tacataccat tgatattcat
    15361 aagtaggtaa tacagcagta caagatagag ctgaattgtt tgcgccacca gtagatgcat
    15421 ctgcatttat attagaaata ggatttgttc catagcaaat ttttacatca gttggtgaag
    15481 ttatagcgcc agcagccaat gttgaataac ttggatgatt atataaacta ctcgtaccat
    15541 tgttacctgc attctgtccc gttccaccat taactgcaat tgttgccgca ttagagaagc
    15601 tcacgcaagg attgtaaaat attttcacac gaccgccgcc gccgccgccg ccactttgac
    15661 cattaacatc actcgttccg ccagcaccac cattgccgcc gtttgcgctt aatgttgcgc
    15721 ccgcaggaat agagattgga ccaaaagcac taattaaaat agtaccacca gcaccacctc
    15781 cgccaccagc accagcgccg cctctgcctg ttcgtgtacg ctcatcacag ccgccagcaa
    15841 ggtctccaca acactcacct gtttctgtcc aatctccacc attaccacca ttgcctccgt
    15901 tacttccatt tgctgatata tttgcacttg ttgacattgt aaatgtattg catgctatta
    15961 atgatataga tccaccaccg ttaccgccat ttgcgccagc agttctgttg tctctgccgg
    16021 tgccaccacc accagcacca ccgccgccac tgcctggtaa cgcagtataa tctgatgcgc
    16081 taccaactgt attgcctcct gaagttgctc cattaccgcc actacctact gcatagatta
    16141 gggaacatcc agcctctcta caagcatctg tgccgtttcc tgtagaacca ttacctccgt
    16201 ttccaccgct gcctccattt ccaccacctt ttccaccacc gccgccgccg gagccaccaa
    16261 ctcttccacc ttcatcatcc caattactac atctttgttt tttgccttgt ccatcattac
    16321 ccttactgcc accatttccg ccaccagcac cagcgccatt accaccagca cctccaccta
    16381 ttgtttttaa ctcacggcaa ttatctttat cagaacacca taatatgcct ctaccatcag
    16441 tgttgttacc ttcagatgac caatagccgc cttgtacacc gccattgccc cctagttcgc
    16501 cactattgtt agcattgata gtaccattaa ttgtaatatt tgtagcatta attactaatg
    16561 gacaaccttg aggaacagta attgtgattc cactgtttaa tgtaaaattg ccatttatag
    16621 aaatattgcc acctaatgtg atgttgttag ctattgtgac attgccagta aatgcagaag
    16681 cacgaactat agttacgacg tttgagtaaa atgtaccatt gcaacttgtt gttctcactc
    16741 tgtaataagt tgttgtagta gggttgatgc tactccatgt ttttgtcaca tcgcctgtat
    16801 ttaatctagc actttccgta tgtcctgaaa cattagccca agtactgcca tcattagaat
    16861 attctaattg attccagcct aatccaccat tccaagataa cgttacagct cctgtactat
    16921 tacatgcttg attagaacta agcgtacctg ctactgcatc actacaagat atatttacgg
    16981 ttgtacttcc tggtcctcct gtttcacgca tttgatattc taaagcttgc gtattgccat
    17041 cgaatgctac attcgtgctt tgtgtagtac aacatgagcc atgctcatta ataaaactgc
    17101 tgccgttgat acgtattcta ctccaatcat ctgcaccaga ttgaatggtg taaatgcctt
    17161 ttgtaaaagt acgtgttagt agtgctttta atgagaaatt atcattcatt gctgtgccac
    17221 aattttggcc tacaccagtt ggaacacctg gaaaactaag attaagagaa gctgaatttg
    17281 ctgcgcttgt acctcctgta tttgttcttc taaaacttga aattggaaat gaataatcag
    17341 tcgtattata ataatataaa ttccatgtgc ttgtgccaac agcagttgca ttgcctaatg
    17401 agccatttgt tccaccggcg tccatttgac attgttggag tgccgctctt gcaccaccag
    17461 agccttcacg catatcaaat actagatttt tgtttccggt taattctata gggtcgctta
    17521 ttgtgtttcc atttccacaa tgccaacacg ataaatgcca tgtgttacca ccatctacac
    17581 ttagtcttct gctgtcgtca ttattttgag tagagaaaac atatacgcca ttactaaaat
    17641 tttttgtggt taaatacctt acagcgtatc catcattatc catacttcta gcgcaatttc
    17701 cacttcctgt tgtgaaacct ggttgaccat ttccccaatt gttatcaatc aaatttgggt
    17761 cagaaccagt agttgtaaaa tgtcccatgt agttgttgtc aacagtaggt tcatcaccat
    17821 catacactaa tgccttccag tttggatagg attgattaaa agatttatca ccagggattt
    17881 gacatatagt gttggtaaca ccagcaccac cgccgccttc tgcttgttgt attactaaat
    17941 tttttgagcc attcaaataa agtgttctgg catcatcaac agttgaagaa ctttgccaat
    18001 tggtataaat attccaatca tttgtgtagt atggatttgt tgcaccattc gagcttagac
    18061 gtatgccatc atctgaagtg aacttaaaac gataaaatcc ttctgtaaaa cttttattga
    18121 cagcatgcat agtagaaaaa ttatcgctac ctatggcacc accacatgcc gtactagtca
    18181 atggcgttcc ccaagatatt cctagattat cagttgaatt atttgcgtat ccaccagtga
    18241 ttgttccttt atattgtgct atatctaaat ttgtagcatt aaatgcataa ccattccaac
    18301 ttgaagtgcc gtaagaaaat aatggtcctt cgtagcagta gctaaaattg gattgcgcac
    18361 cgccaccacc ttcataaaat tcgaaaataa gatataggtc gccaccattg tgtgtgtata
    18421 ttgctgaatg atcagcacaa cagctgctcc acgcatttat ctgataggtg agaccactaa
    18481 aataagtaat taaagtatta cttattgcta atcgtttgcc atcgtcattg ccgatagtaa
    18541 atttatatat tccggcagct aaatttggaa gacgcattag ataacgaata ctaaatgaat
    18601 tagcatctgt tgcacaagca ggacggcttg ttgctgtatt tccccatact cgattaaagt
    18661 ttgctgattc tgttacatat cctttgtagg tattgaatga tgttccatca tacacatatc
    18721 ctgtccacga attgtcgcta taggaaatct gattgccgcc agctatgcat gcaagctgtg
    18781 ctagagcagt ttgaaaatta agcgaagcca aaagtataaa tagtaaactt gctatgcgta
    18841 ttttacgatt gatacattcc tttttaagaa taacggattg tgtgttagca aaagcatagg
    18901 catgtagttt gtggcatgaa ttattaaata ataaactgcc aagcgcttgt aaaagtttgt
    18961 gcatgtaatt agagtttaag aagtaattgt ttacgtttac gagcgccaaa tgtagcagat
    19021 tatatttcta ttcaatatga cagatatcat taaaaattac gatttaacat acagctaata
    19081 ttctatcagc gataaattgt tgaataattg attattgtca ataaataact gaaaatcaaa
    19141 atattatatt ttttattaag tgtgtgatct tttaagaatg tagaatttat ttgtagtttt
    19201 ttttatttca gtaagcgata ctttttttga tggaaagttt gttttataaa taattcgatg
    19261 agttttttta ttgattatct aaaaatctta gcttaccttg tagcattatt ttggaacgct
    19321 atttttcaat actactttgt gttctgctgt ccaatattgt gtatgcgcaa attggcggaa
    19381 agaatgtgta tgactttttg caatttccga cgtcttcgcg catggaggcg ctcggtggtg
    19441 gcgtgcaaag tataaaatat gctaaagacg ccgatttaag tatggcgatt tcaaatccgt
    19501 gtttcatcga tagttctttt cataaacaac tggcttttac aaatgcattt tatacagata
    19561 aaacaaatat cggcaacatc gcatttgcct attatcatcc taaaataaaa tcagcgatag
    19621 catatacgtt tcagtttgcc tcttatggca aatttgatgg tcgagatatt gctggtaatt
    19681 ctacaggtac atttcgtgcc gcagatttta atttgcaagt aggtattgga agaagcatac
    19741 gtcgttttta ttatggtgta aatataaaat ttatcgcatc acacgccgaa agttatacat
    19801 cattaggctt tgctactgat gttgctattg gctatcatca tccaaaaagt aattggacat
    19861 ttgctgccat tgtgcgcaac gctggtgtgg aaatgaaacc ttatatcaaa gaaagtggaa
    19921 ggcaaaaatt acctatacaa ttagatattt ctttttctaa acgatttaag aaattgccat
    19981 taacactatc tgtcactgcg cataacttac aaacgtggaa tttgaaattt cctgaagaag
    20041 agcaaaccac attatttgga acaacaaaga aaaaaagtaa aggcgcagaa gtggttgata
    20101 atatctttag acatctaagc tttggtgcgg aagtgcaagc aggaaagcct gtgcgtttgc
    20161 gtttaggtta taatcatttg cgaagacaag aattaggcgt aggcaagaaa aaaggatttg
    20221 caggaatttc agctggcttt ggtgtaaaca ttaaacaatt tgcattcgac tatgcctatg
    20281 cgcaatatca ccgaaccggc agcgaccacc aattgtcttt tagaataaaa ttagacgaat
    20341 ttggcaagaa agcgaagtag aagtgaagtg aaaaaaatac gtttttaaaa caatgagcaa
    20401 taaaataaat atcgccatcg acggatattc tgcatgtgga aaatcttcca cagcaaaacg
    20461 cgttgcacaa cgtttggcat tcttatacat agattcaggt gccatgtata gagcggtaac
    20521 actttatttc caacagcatt taatcgattt agataatacc ttagccattg aacaagcttt
    20581 gccaaaaatc actatcaatt tcaaaaaagt cggtgatgta catcatactt ttttgaacga
    20641 tgttgatgta gaaaatgaaa tcagacaacc acgcgtggca gcacttgttt ctgaagtggc
    20701 agcgatgagc aatgtacgca aaaagctggt tgagcaacag caacaaatgg cacatcaaaa
    20761 aggtgtggtc atggaaggcc gtgatattgg cagcgtggtg atgcctgatg cagaattgaa
    20821 attttttatg acagcagatg tcgatgtgcg aacgaaacgc cgacaagatg agctacttaa
    20881 aaaatctgga aaattatttt cattagaaga actcaaagcc aacttattac atcgcgataa
    20941 aatagattct actcgcgcgg attcgccatt gacaagagtt gcagatgcta tagaaattga
    21001 cacaacgcat cttactttag aaaaacaagt tgatttaata gtagagaaag ccttagagcg
    21061 tatgatgctt aactaaagat aatttttttg tagattcaag cgatatcatt ttttcctact
    21121 tattcttaat tgtaataatt ttttaaactc aaaaaatatg ttgtaatttt aattacaatg
    21181 gaatcatcga taaaggaaat aaaaatagac agcacatcag acgatgcgaa aacaaaaatc
    21241 attcgcaaag aagtgcagca agccagcaaa gaaataagat taaaatatcc gattttacaa
    21301 caccaaaata caattggatt tttcttgttt gcattttcaa tagctatgat attattgacg
    21361 gcttatttat atctaagcaa tgcaataggt tttgtaatta ctacaatttc cgttgctttt
    21421 tggacatcga tattgcatga gctagagcat gatttaatac acttcatgta ttttaagaag
    21481 aataaattta tacaagattt gatgatgctc ggagtttggg tttttaggcc aatgacgata
    21541 aatccatggc taagaagaga aatacatttt caccatcatc gtgtctctgg cacacgcacg
    21601 gatattgaag agcgcggcgt tactaatggc gagccttggt cgtttaaacg ttttatcacc
    21661 actgccgata tttttttagg aggtgttttg cgcggaaatg tcattagaaa agatattatc
    21721 gacgctgtca aaaaaggtga gttgcccaaa gctcaagcgc ttgaatttaa aaaggtaaag
    21781 ctttatggta tgctgccatt tacactttta ttgtatgcta tctggtatgt ttttttagca
    21841 cattatacta ttcattttat tacacaattg atggacattt cctatcaatc accagcatgg
    21901 ttacaatcgc aatttacttg gataaatcct ttggtggcga tattaatttt tccaaattta
    21961 ttcagaatgt tttcacttca ctttattaca tcaaacatgc attactatgg cgatgtggaa
    22021 tgtggcaatg tgatgcagca aacgcaagtg ttgaataaat ggtattttat tccatttcaa
    22081 ctattttgtt ttaattttgg aagtacacat gcgattcatc attttgtagt gaatgaaact
    22141 ttttatgtgc gccaattaac agcaactacg gcacacaagg taatgcgaga gcaaggcgtt
    22201 cgattcaatg atatcgccgc ttttagacgt gataatcgtt tttacgaaaa acaattacat
    22261 ccagcctagc tagaaaatat taggcaatag tcttgttgtt tttttctaaa gaatctaaga
    22321 attctttttt cttgcgttta gaaatttcca ctttgctacc gtcagtcatg atagccaaat
    22381 tgtcgccatg atcgatttct ttcatatgtg caagattaat taaatgcgat tgatgtatgc
    22441 gtaagaaatt ataagacaat aatttttctt caaaattttt taatgttttt gctactagta
    22501 cttttttatt gcccactaag aaaaacaaag tgtaattgct ttcagattgt aggcgaacaa
    22561 tatcattgac tttcaaaaaa atcatgccgt cttttgtagg caccataagt ttagaatctt
    22621 gctgttgttc ttgttttatt ttatcgagat agccttgtag ctgttccgct tgtttgagtg
    22681 tctctttttc ttccaaacgt tttgctactt tctcgatagc cttttttaat tccttttctt
    22741 ttatcggttt taataaataa tcagcagcat tattttttat ggcactgatg gcatatttat
    22801 catgtgatgt agtaaaaata atttcgaagt cagtagattc taattcattt aataaatcaa
    22861 taccttttcc gtctgctaat tcaacatcta aaaaaatcaa ttgaggtcgc acacttggca
    22921 atatcttttt tgcttctctt tttgaattgc aaacaccatc aacacgaatg gaaaatcctg
    22981 tttcctttat ggcatttttt aatatttcct gaaagattgg ctcgtcttca attattacgg
    23041 cactaatcat aatttgtaat tgcgtatgct aaatatattg aatattgatt aattttaata
    23101 acttttcatc atttctttta tgtaatgaca tcaaagttta aaattaaagc atcatcattg
    23161 tctgacgacg aagtgcgcga attatacgct aaagcagccg tatgcaaaca agaaaagaat
    23221 tatatagatg cactggattt ttacaataaa attatcgatt ataaaaattc ggaagaatgt
    23281 gtggaatgtg ctaaagtata tgaagatatt ggcgatattt attttgaact aaaagatttt
    23341 caaaaagctt tcgaaaacct tgaaaaagca ttacatatct atgcagataa tgaagagtat
    23401 gaactgcaat taaatcaata taaaaaaatt ggtagcttgc aacaaggcat ttggcagttt
    23461 aaaaaagcga ttgaaatatt ccatcacgga ttgagtttag cgacgcgact agataaaaaa
    23521 gaaaaagtat tagagtttga attgctatta ggtaatgtct tcaattgggc agatcagtta
    23581 gaagatgccg aacaatattt ggtgagcgcc attgaaaaag aaaagcgaat taattcgcct
    23641 ttgataaagc ttcgcgcaca cgttagttat gcgattctac tacgcaaaat gaagcagttt
    23701 caaaaatcag aaacgtattt taaaatgggt attcaattgt cttcggaaaa caacaatgcc
    23761 tacatgatgg acatcaccaa aagttatggt gtattgaagt acgaaactgg acattacgaa
    23821 catgcagaag aattgttagt gaaagctgaa aaaagcgcag aatttgaagg caatgacgcc
    23881 actcgtgccg ttatttttga atacttagcg atgttatatg agtataagaa agactatgaa
    23941 aaagcatatc actatatcaa taaattttat ggcagaaaat tagaattatt agagaaaggt
    24001 tattcagatg acaataatat tttgcaagca aaaataggct tggaaacggc caaacgagaa
    24061 cgtgtaatcg ctgaggaaac agcaaaagca aaaagtttgt ttattgctac tatatcacac
    24121 gagattcgaa caccaatgaa tatcatttta ggtacagctg ctttgatgtt gaatgacagt
    24181 ccaaagcctg cacatctaaa atatttacaa actttaaaaa aatcaggcga gaatttacta
    24241 ggcatcatca atgatatttt agatgtttca aaaattgaag caggaaaatt agaagtcgaa
    24301 tatgaacctg ttgaattgtc cacactcttc gatgaaatca tttctatttt agaacaaacg
    24361 gcacacgaaa aacaactatc cttgagttat actgttgatg aaaaaatcaa ctttgccatc
    24421 atgtctgatc cattgcggct tacacaaatt attacgaatt taatcagtaa tgcgatcaag
    24481 tttacagcgc aaggaagtgt gcatttgtcg gcaactttat tgcctaatga taagctgcaa
    24541 atcgttgtgc gcgatacagg catgggcatt cctaaagaca agctgaaaac tatatttgac
    24601 caatacgaac aagtacgcac aaaagtgcag aaaaaatata aaggaacagg tttaggtttg
    24661 gcaatcgcca agaaattagt agaaatgatg caaggcaata ttgagataaa aagtaaaata
    24721 aatataggca caagcttcat cattacattg ccaatcgaaa aagcagccat acagcaaaaa
    24781 gtcgatattg ctactttaaa acgcgatgca gctttcttaa gcgataaagt aattgtaatc
    24841 gtagatgatg ttgaagacaa tagaactgta attcgagaaa cacttaaatt ttttaatcct
    24901 gccgtagtta ctcacgaggc cgcagatgga cagcaagcgc ttgatttatt gttacaactt
    24961 acaaacgtgc accttgtcat catggactta gatatgcctg ttctaaatgg ctttgaagct
    25021 ttgtctgcca ttcgcaagca taagaaaatt aaaaaattaa aagtacttgc cagtacagca
    25081 agtttgatta caactagtga tgatgaattt atagaatttg gcttcgatgc gtacttgcct
    25141 aagccttttg aaatcgatag ttttttcttg cttttagaga aattcctaca ataattttta
    25201 tttcaataaa atgaaattat tgaaattaat ctctaagatt caaaccaacg acatcttcat
    25261 aagcaacata gagcgcacat atagagcttg gaattgttac caataaacca acgaccaaac
    25321 acaatgcacc tgctgcatta aatagaccaa gcaagagtag aaaccctaac caattaaaga
    25381 actttttgct tattaatttt ctactgaggt ctaatgcttt tattggttcc atcttttcaa
    25441 acaatacaaa aaatgtagcg aataaaaaca aaacggaaat cgcaattgat gcaatgccaa
    25501 caaggccagc gcgtccagca aagcccgtca tagattgcga aaacgcagag atatcgccaa
    25561 aattactgat gttgattttt ggtgtaatgc ctaaaaatgc cattttaaaa tatgcccaac
    25621 ctcctaaaag taacatcaaa gcggctgtta taagtgtagg aattaatgta acgatgagta
    25681 aaggcatata attttttgtg ccatcaaaga agttgttaaa cgaatgcact tcctttttgt
    25741 ccaccatttt tgcgacgatg aatccgccag caatgatagg tgctatcaat gcaaagacga
    25801 taccttttaa aaatggtatc atcgaaatga tcatttccat cacagcaact aatacaataa
    25861 aaccaatgaa tgggccaata ttttgtttga aaatttcaaa tccttttttg atgtaatcga
    25921 cggcgtttgt atcatagcca tttgtcaaaa gtgaactcaa agatttacct tctttgttcg
    25981 acaacatatc gctgtctaat aattgatttt ccataatgtt agtttttgct tattgaaaag
    26041 taaataaaat aaacaaacat tgcaggtttt tttataaaca aatcgaaaat aattaaagct
    26101 ctaatccagg aattgtattc aaattaacct ttgggaatcg cgtttacata gtccatttta
    26161 tagctcataa tatcggcaat cgagcgagct cgaaattttg ctttttcggc aagttcacct
    26221 tcaaacagtg tattaacggc ttgttcccaa tagtaatacc aaagatcgaa atctgctttt
    26281 gtaagcggtg tgcgtttatt ggtgtcaaaa tgtttttcgg taacattgcc tctgtagttt
    26341 gctgcgtcta agagtatgcc ttcccaaaaa tctgtgacta aaggaatgtg tgtttctaag
    26401 tgtaatggac taatttcctt gaatattttt cccaaacgct catcttcaaa agttaattta
    26461 taaaacaaaa taatcacctg ctcaatgtca tttctgttgc ttatatcttg tttcatattg
    26521 acgatttttt tggaataatt ttgcgttata ttgcttccaa agaatctaat gcagtgatgg
    26581 tgtatattgc ttttaattct ttttttgtac cgctaataat ccatctgttt ttattgtaaa
    26641 tattaatgct atcatgcaaa tatatactgc cattataact aaatgtatcg gctttagctt
    26701 tgccattgac aaaaaataat tttgtaaaat tgccatttgg atatggtata atgctgtcca
    26761 cataacggca atcctcattg attaatttgg tttcgcctgc tgcaacggaa aacgaatcga
    26821 ttgaaaaata aaaaccagag tctattattt tataatttcg aataaaaacg gtagatgata
    26881 aatcattttt tacataaaat tcgctgcatg caatttctgt atcgatgagt ttgcaagcag
    26941 ataatcctaa gcaaaaaagt agaagtaaag caaagtgtcg catatatttt atttagtaat
    27001 aaagatacta aagaaaataa cttgtacaat taatctttga tttttagaat aaagagtcaa
    27061 tgttttttaa ttaaataaaa aaccgccaag aaattcttga cggttttaat ctaattattt
    27121 gcgagtttta tttcactaat aatttatgtg aaggagcaaa ttttgtaagg ttaatacctt
    27181 ttactgctgc cgtgtattct tcaatagtcg gtgttctgcc aagcacagtt gacaatacaa
    27241 ctactggtgt ggaagataat aaagattctc cttttttctc atcagaatct tctaccactc
    27301 taccttggaa taaacgagta gatgttgcca ttacggtatc gcctttttct gctttttctt
    27361 ggttgcccat acacaagtta caaccaggac gttctaagta caacatattt tcatacttcg
    27421 ttcgtgctgt tgattttggg gcgctgtcgt caaattcgaa tccagaatat ttgcgcaaca
    27481 cgtcccaatc gccttcagct tttaactcat caacaatgtt gtatgttggc ggtgctacta
    27541 ctaatggtgc tttaaattct actttaccat tttgtgcttc aatattcttc aacatttgcg
    27601 caagaatttt catatcgcct ttgtgtacca tgcaagaacc gataaatcca agatctactt
    27661 ttttagtgcc accataaaat gaaagtggtc taattgtatc atgtgtatag cgtttagaaa
    27721 cgtctttgtt gttcacgtct ggatcagcaa tcattggctc agcaatcagg tctaagtcca
    27781 ccaccacttc tgcataatat tttgcattag catcaggtct taaagctgga ttttctccag
    27841 atgttatttc ggcaattcgt ttgtctgctt tctcaattag tcctcttaaa acatggtttc
    27901 tgttgtccat gcctttgtca atcatgattt ggatacgcga ttttgcaatt tccaatgatt
    27961 ctattaatgt ggcattttca gaaatacaga tagatgcttt cgctttcatc tcggcagtcc
    28021 agtctgtaaa tgtaaaagct tggtctgaag taagtgtgcc gatatgtact tcgattattc
    28081 taccttggaa tacattttca cctgcaaatt gttgcaacat ttgtgcttgt gttgcgtgta
    28141 ctacatcgcg gaagtccatg tattctttca tgcttccttt gaatgtaact ttcacagatt
    28201 ctggaatagg catcgaagcc tcgccagttg ctaatgcaag tgctacagta cctgagtctg
    28261 caccaaaggc tacacctttt gacattctcg tgtgtgagtc gccaccgata ataatcgccc
    28321 agtcagacac tgtaatatca ttcaatactt tgtgtataac gtcagtcatt gcaggataga
    28381 tacctttcgg gtcgcgtgcc gtaattaagc caaagtcatt catgaacttc attagttttg
    28441 gaatattggc ttgtgctttt ttgtcccaaa cggaagccgt gtggcaacca gattgatacg
    28501 caccatcaac gattggagaa atcactgtag ctgccattga ttccagctct tgagccgtca
    28561 ttaaacctgt agtatcttgc gagcctacga tgtttacttt aacacgaaca tcagaaccag
    28621 tgtataatat ttttccagct gctacgccta cagcgttttt attgaatatt ttttcaaccg
    28681 ccgtcaagcc ttgtcctttg atagtaattt ctttagaagg cgcaaataca ggcgtgcttt
    28741 caatgccaag cgtttgagct gcaaaagttt gaattttttt accaaacaca atcgcataag
    28801 agccaccagc ttttatgaat tccatttttt gtggcgtaag tgcttttgaa atatcaatta
    28861 actctctgtc gccgtttaat aattttttct tttttgtatt gatggtaagt tcagtgccag
    28921 tagcaacgct gtatttttct tccaaaatcg gttcgccatt ttcattaagc ataggtttgc
    28981 catctgcatc tactttcttt acccaatttt ttaagtcgat accgataccg ccagttacat
    29041 caactgttgt taagaaaata ggagaaatac catttgttcc gccaactata ggagcgatgt
    29101 ttacaaatgg cacataaggg cttgcttgtt ttcctgtcca aagtgctacg ttatttacac
    29161 cagacattcg cgatgaacca acgcccatag ttcctttttc agcaattagc atgatgcttg
    29221 cagctggatg tttagcttgc aaagctacaa tttcagcttg cgcttctggc gttatcatgc
    29281 atttgccatg caattcacgg tctgagcgcg agtgtgcttg attgccagga gaaagtaagt
    29341 cagtagagat atcaccttcg ccagcgatat atgtaacaat ttttatttct tctggtactt
    29401 ccggtaattt tgtaaaaaat tctgctttcg catagctttc cagtatttct ttagcaacgg
    29461 cattgccact ttcataagca gctttcaaac gctccgtgtc tgcttcgtat aagaatactt
    29521 gtgttttaag aacatttgct gcttgctctg ctttggcagt gtccgcacct aaagcaatat
    29581 ccaataatac tttgatagat ggaccgcctt tcatgtgtga aagcaattca aaagcgaatg
    29641 ctggtgtaat ttcgtttaca actacgtttc ctagtatgat atcttttaag aaatcagctt
    29701 tcacaccagc tgctggcgtt gtgccaggca atgtattgta aataaagaat tgtagagagg
    29761 catctctgtt ggcgttgctc aaatctttga tttgttcaat aatttctttc aataattctg
    29821 ctccatcaat tggttttgga tgtaagcctt gttcttttct ttcatcaatt tccttgatgt
    29881 aatcgttata aatactcata taatataaat ttattaaata ttagaataaa aattagatat
    29941 atatttcact gcaaatttac aaaaaatgaa gaacatgcta aaatttttaa tcttatgtag
    30001 cgatagattt tctttattta gtattattta tacgttttct aattaaaatc ttaaaatttt
    30061 gttagaatat acaattacat taacttagac tatgcctaca ttttcgacta gaaaagctat
    30121 tgaaaaagcc atcactttat ttaataaaca cgcatataat gctggtttag attttgaagt
    30181 atatacatca gatacacatt gcttatatgt tcaagctagt tttgatttcg catattatgt
    30241 aaatgtagat atcgaatgta gagaagtttt ttatacaaat attgcatcga acgcaaaatg
    30301 gcctgatgct tggagtaatg accagctttt tttattagat gacgaggaat tggaaagtat
    30361 attaaacttt aatgaaataa gcgtagctga tgacaaaaca gtatttggta tcgtattcaa
    30421 catcattggt aaagaaaatt atcacattaa tggtttggtg atatgctcgt ctttatattt
    30481 taattggcgt tatccgaatt ataatgatca gtatttgatg tgatgacgct gcttgatatt
    30541 tgtcaataat taatatgtgc atgcatatct ttaatttatt ttcctaattt cagtattcga
    30601 agattttact aaccaaataa tctaattaat taaacatgaa aaaatcaatc ttttcactac
    30661 ttagcgtagt attagtagcc agtactttgt tcgtatcgtg taacaaggta gcagatgtca
    30721 ccactgcacc accattagaa gaacgcttat ctaaagacaa agtattgtca gatgctattg
    30781 ctgccgcaac agatttgtat ttaaaaacat cgaatggtac tttaagtgat ccaaacagca
    30841 ttcctgcctt agaagctatt gcagtaaaaa ttaataataa aactgcaagc acagcggatt
    30901 atcaagcagt ggaaaatatt gtaggtatgt catacaatga ttttattcag gaattacaaa
    30961 attttggaat tgctttatct aaggtaaatg aaaaatatcc tgagttagct aaaatgaatc
    31021 aagcagactt gcaagcaaca tttaaaaaat caatcgagtt gaacccgaca ttgcaaaatt
    31081 cattaggtgc atcagcatta gttgacggaa gagttactgc atgtccttta cgcgatatct
    31141 gtaatttagc agtgacgcta acaagaatat ttgctggcga tgctatttgt gcagccatca
    31201 acgtgactac gattccagta gttggtggtt tattatgcac tttagtgctc aatctaggtg
    31261 taggcttatt gacagctatt tgcaatgcat taccttgtta atttttgaat cttattatag
    31321 tgagccacca ttaaggtggc tttttttatg aaatattgca aatactgtaa tattttgttg
    31381 tggctaaata ccaatattga tagcaaatct tatctttaaa caatgaattt tttagaagta
    31441 gtacaacttg cctatcgctc ggttcgcaca aatatcgttc gcacgattat tacatgttgt
    31501 attattggtt ttggtataat ggcattggtt ggtatcttga catcggtcga cggattaaaa
    31561 ggatatctgt cagagagttt tagtagtatg ggcgcaggta cgtttaaaat cagaaatcga
    31621 ggtttaggtt ttaacttaga tgccgatgct gacgaagcac agaaagtctt taaaagtatt
    31681 tcctattttg aagccgctca atttaaaaat aagtttaatg ataaatatcc tacgtctgta
    31741 caatatatcg gtgcacaggg tgcagtagtc aaatctggag ataaaaaaac caatccgaat
    31801 attttagtca ttggttcaga tgagaattat gttggcaatg aaagttatac cattttacat
    31861 ggcagaaatt tcacagaaac agatataaaa tatggtacca aggtagtgat gcttggttat
    31921 acggttgcaa aaaaattatt cggtgccaat tatacaatgg aaaataaagt agttcgcatt
    31981 gatgatgtaa aattcaatgt tatcgcagtc ttagatgaaa aaggttcgag ctttatcacg
    32041 acagataata tggtattagt acccttatca acagcacgac gattatatac ctatcctaat
    32101 ccttcttttg tgataagtgt aaaaggcaaa gatccagaaa atatgcaacc tgcagaagat
    32161 gatgcaattc aagtgatgcg aaatgtacgt aaactacatc ctggcgaaga gataaatttt
    32221 gatattttaa aaagcgatag tatttccgga atgtttgtcg aaaaaatgga atatgctacc
    32281 atggcaggct ttgtaattgg tattattaca ctcgtaggcg ctgctatcgg cttgatgaat
    32341 attatgctgg tcagtgttac tgagcgaaca aaagaaattg gtactttaaa agcaattggt
    32401 gccacaaata atacgatacg attacaattt ttattggaag ctatagttat ttgtcaactc
    32461 ggcggtttgc ttggcatagt gttaggtatt gctgccggaa atgcagtttc tttatccgtt
    32521 ggaggcactt ttgtaatacc gtggatgtgg atactagtcg gtgtcgtatt ttgcttaatt
    32581 gttggcttgg tgtctggcat ttatccagca tttaaagctt caaaattaga tccgatagag
    32641 gcattgcggt atgagtaata tttgatgtga ttcaaataaa aaatcgccaa acgtaagttt
    32701 ggcgatttta atataattag caactaatta ttattctgct acaacatcga attcgatttc
    32761 tgtagaaact tctttataca aatttactgt tgctttgtaa gtgcctactt cttttacttc
    32821 accgatgatg tgaactcgtt ttctgtccac tgttaagtcg aattgttttt tcaaagcttc
    32881 tgcaacttga atgtttgtta cacttccgaa taatttacca gtagtacctg cttttgctcc
    32941 tactattgtt ttagaagctt tcaatttctc tgctaatgct ttataatctt cgattaattt
    33001 agcaattttt gcagattgtt gcttcttgat ttcttcgttg tgcttcttgt tcgaagcatt
    33061 tgctacttga gcaaatcctt gtggtataag gtagttacga ccgtagccag gttttactga
    33121 cactacatcg aatttagtac ctaagttttc tatatctttt aataaaatga cttccatgat
    33181 tgtattattt taaaaggtca gtaacataag gtaataaagc caagtggcgc gcttttttca
    33241 cagcttgtga aattttacgt tggaacttca atgagttacc agtaatacga cgaggcaata
    33301 attttccttg ttcattaata aatttcaaca agaactgagg atccttgtag tcgatgtact
    33361 tgataccgct ttttttgaat cggcagtatt ttttcttttg caagccaatt tttggcgagt
    33421 ttaagaactt gatttcgtct tgtgttgcca tgttatgcct ccgctttttt agatttacca
    33481 cttagtttgt ttctgcgttg ttctgaatac gccaacgcgt gtttgtcaaa tcgtgtagtt
    33541 aagaaacgca tgatgttctc atcacgttta aattctactt cgaatttgcg gataaattga
    33601 gcgtcaacct caaattgcaa caaagtatag aaacctgttg tctttttttg gattggataa
    33661 gctaattgct taatgcccca atcttctttg tgaaccactt ttccgccagc cttctcgata
    33721 aggtctgcgt acttttttac ggttcttgtg tactcatcgt tactgagcac cggcgttgta
    33781 ataaaaaccg tttcgtaatt gttcataatg gattaatttt aaattccgaa aaatttcgga
    33841 ttgcaaagat agtaattaat tagttaatag ggaaatgata tcgataaata tcgaagtgaa
    33901 aatgaggatt ttatgttaaa ttttagtgat attcttcaaa aaattcttat aaaaccaaaa
    33961 aaatctattt ctcagcatca atcatttgta ttaaatttta tccactcgct tctgatgtcg
    34021 tccgccttca aattccgtag tcaagaaagt atgtaccatt tctgctgctt cgtcaattgt
    34081 gatgaaacgt gctggtaggc aaataatatt ggcatcattg tgctgacgag ctaagctggc
    34141 aatctcattt ttccaacata aagcagctct tattttttgg tgtttgttcg ctgtaattgc
    34201 aacgccattc gcagacccac atatcagtat tccaaacgct acatttgccg tttcaatttc
    34261 gtttgctgcg gcgtgtgcat aatctggata atcacaactg tctaagctat ttgtgcctac
    34321 atcaataatt tccacattat ttttagctaa ataacttttg attgcttctt tatattcaaa
    34381 gccagcatgg tctgagccaa tgacaatttt tgttggtaaa ttcatgcgat aaaattacaa
    34441 ttttagattt aggatttaag atttttagct tattaaatta atatctctta actttcaact
    34501 tgtgaatcag aactccttgc aaacaaaatt agaagcacgt gtcttgttta tagacatgaa
    34561 tagttttttt gcaagttgcg aacaacaaac caactattgg ttgcgtggca gaccggtagc
    34621 cgtttgcgta tatactggta aatatggttg tgtgattgcg ccatctatcg aagcaaaaaa
    34681 acgaggtgta aaattgggca ttcgcctaaa tgaggcaatg aaaatttgtc cagacttagt
    34741 gcctttagaa acaaatccag ataaatacag aacctttcat gcgcgtatca tgaaaattct
    34801 aaaacgctat gccgatgatg tcattccaag aagcatcgat gaagctgtaa tggatttgac
    34861 ttcatataaa aatatttatc cagatgtggt ggaagtggca aagaaaataa aaaaagatat
    34921 cacagaagag gttggcgatt atctgaaatg ttctataggt attgcaccaa atgctttttt
    34981 ggcaaagttg gcatctgata ttcaaaagcc tgatggcttg acgattatta cacctgaaaa
    35041 tattgatcag gttttaaaaa aattggaatt aacagatttg cctggtatag ccaaaggcat
    35101 gaaactgcgc ttaaataaag caggaattta tactccttat gatttacgac attctactcc
    35161 agagaagctg cgagccgctt gcaaaagtat tatcggtttg cattggtatc atcgtttgca
    35221 ttttcaagaa gtggatatgc acacgactga tgagtacaaa aacatgtcgg caatgcgtat
    35281 ggtgtctaaa gagcaacgaa aagaggtgca gcacctcaaa gatatcatga tgatgctttg
    35341 catcactttg gaaaaacgca tgatgaaaaa acatttgttt tgcaagcacg tgcaattttt
    35401 tattcagtat gaagaaatgg aaacaggcta tcacgataat tttcataccg aacaaccttt
    35461 gcaagatggt atggaattat tccatatttt attggatagg atgaaagtat ttgaacaaaa
    35521 aaataaatgc tcacaacaat tactcaataa caatattatt agaattggtg ttgttgtgca
    35581 aaattttatt aatgacgaat ttttacaact taattttttt gacaatacac atatcaaaaa
    35641 caacaaagtg cgaaaagcta tttacgaaat taaagacaag tatggtggca aaaccatcaa
    35701 acgtgcttct gaattaaatg acgaagatac cgctgatgac gtcatcggat ttggaagcgt
    35761 aaaagatgta aacgaaagtg attttggata atttaagaat taatgctgca accaattata
    35821 actaatttgt atctttaaat aaaataaaaa tatcatggga aaatttgtaa ttaaaacaca
    35881 aaaggacgga cagttctatt ttaacttagt tgccggcaat ggacaatcga ttttgaaaag
    35941 cgaagcttat acgactaagc cagcttgcct aaatggcatt aactctgtta aatcaaatgc
    36001 tgctgacgat aatcgttacg aaaaaaatga atcaaaaaac ggcaaacctt attttaatct
    36061 aaaagcagga aatggacaag taattggcac tagcgaatta tatgaaagcg tagccagccg
    36121 cgacaatggc atttctagcg taaaaaataa tgcaccagaa gcaacaattg acgatactac
    36181 tgtctaaaaa ttgtactgta tattttttgt gaaagattcg catcgcgaat ctttttttta
    36241 ttcatttctg aacttagaaa taaatagcat cagcgcaccg cttaataaaa taagagcagt
    36301 tgctagtaag gcttcattgc tgtatggaac aagaatatat tttatggaag ctacaccttg
    36361 taacaacaat ataaattcat ttaaaaaaac acctgctaca aatatccata aacccaatgt
    36421 gctgagcttg gtcactttga tatgtttttc tgtaaaaata aatccaagca aaaacaaaga
    36481 aataacacct aataaaacta agtgtaaata agcaataacc actgatctaa agccaaatgc
    36541 ccattgccct aatgcaggaa tggtagagcc aatttgtaat atcagtttaa tacttaacgc
    36601 cattgcagat aagatgagca cccaagcccc atgtgaagag aatttatctt tgagcaattg
    36661 acggtgttgt aataaaattt gtataaacat ataccatgct actacttgca tgagtgctgc
    36721 tatgatgact attccataga gccaacttgg cattttcatc caaagcgtcg atagtaagta
    36781 ggcaggtaca catgagctgg caaataacca aaatatataa cttaactttt tgtgttgatg
    36841 tacaatacct gctggcaaaa tagattgtaa taaaccagca catgcgaaga aaaaccaacc
    36901 attgtattga aaatgtaaat agtaataaat agaagctaag taccaatttt gatgcgctat
    36961 cctattcgac atcatatagg caagcgcaaa ggtgccgata gatgaaataa cagcaaatat
    37021 caatgcgcct ttgaaccata aatgagtggt gttgtggctt tttattgacg ataaatcttt
    37081 ccagtatttg acagcaaaaa agtaagatac aaaaacagat aaagtagaaa aagtaatgga
    37141 cacaaatcca tatccttgta ttggaaaagc aagcagcatg ccatacgcac taagcaaatt
    37201 gccaatcagt aaatttctat aatcgatgaa tttattttca tttttttgtt tagaaagata
    37261 ggcaaccatt aataccatta atgtctgtgt tacccagcca gcaaatgcaa aatgcgagtg
    37321 gctgtgttgt atatgttttt gatctaaaaa tggcaatgaa tacaatattt tatatcgaag
    37381 tatagtgcca agtagtgcca caatggctat atttaataat gaaattttga gatatcgtcg
    37441 aatgtatgag tcggtgttca tattgtgtgt atttctattg caaagataga agaaacagat
    37501 gtaagatttc aaatactatt ttcattattt aacataaata cctttcctct gtgaatatac
    37561 aactgccctt ttttgctgag gttttttata acacgaatga ctgtttctac tcgaaggcca
    37621 ctcatctcag ctaattgttg tcgagtgaag tctattttaa aatcggcatg tccattgtgt
    37681 cgtgcttctt tttgcttaaa gtgatgtaat aatgtcaata tgcgctgttc tggtgtatga
    37741 taggcaacat cttttaaaag taaaaactta tatcgcattc tttgtgccaa gagtttatta
    37801 aaactaaata gaatatcagt attttctttt agcaataatt ggaaagcact ctttggtaat
    37861 tgcaaaataa tacaatctgt ttcggcaatt gcacttgtag catagggttc gccgtcaaat
    37921 aagggtaact ctccaaaact ttcaccaggt tcaataatcg attgaatgaa aatttttcca
    37981 tcttcatcta agtttaaaca gcttattttt ccttgaacta attgataata gtaatagcat
    38041 tttgcacctt cacgaaagat atattctcct ttttttacgc gcttatatac ggcaccaagc
    38101 gttaataaaa tatcttcatg aatcatttcc atatacagtg tgtctttctc aacaacatat
    38161 tattataaag gtatatagaa acataggtgc taaatatgac attcggcacc caataatatg
    38221 atataaatca tacgccaaaa tggtcaaatt ttgacaaaac atgacgataa tcataattta
    38281 ttttattaac ttgtactaac attgtacacg aaaaaaaata tcaagtatga aaaaattatt
    38341 tttaattatg tctattaccg cagctatggt aattattaat gcatgtaatt caaatccgaa
    38401 caaagatgac ttctctacgg aaggtactga aagcacagaa agtacagaag caccagcaac
    38461 tactgaaaat ggaaatcctt cttacgatcc aaaaagaggt gaaggtaaat tcacaaacgt
    38521 agaagttggc gcaacattag atgcagcatt agcatcaagt ggtgaaaagg tatatggtgt
    38581 taaatgttca tcttgccata aattaactga cgaaaaatta gtgggtccag gttggaaaga
    38641 tgtaactaaa aaaagaacac cagaatggat tatgaacttc attacaaata cagatgttat
    38701 gttggataaa gatccagaag cacaagcaca attggaaatt tgtttagtga gaatgccaaa
    38761 tcaaaatctt tctgatgatg aggcaagaca actttacgag tttatgcgta aaaatgacgg
    38821 cgtaaaatag tcaagacttt ttaaactaaa aataaatcat atgaaaaagg gcatattcgg
    38881 gaccttagtg gtagttgcta tctctatgct aacgttcaca gcatgtaaac caaaaaatgc
    38941 aggaagcgct gtaagtggcg atgctgctca aaaagtgtac gtcgcaccag gaaaatacga
    39001 cgaattctat aacttcgtgt caggtggttt cagcggacaa ttaagtgtgt acggattacc
    39061 aagtggtcgt ctgttacgcg tgattcctgt tttctctgtt gacccagaaa aaggctgggg
    39121 ctacagcgaa gaaacaaaac cgatgttgaa tacatcgcat ggctttgttc catgggatga
    39181 cttgcaccac gttgaaatgt caatgactga tggtgtagct gatggtaagt gggtttttgt
    39241 taatggtaat aacacacctc gtttagcacg tatcgactta aaaactttcc gtacagcaga
    39301 aattttagaa ttaccaaata gtggcggtaa tcatagttca ccgttcggaa cagaaaattc
    39361 cgaatatatt gttgcaggta ctcgctttag cgtgccagca gataatgtaa atggcgacgt
    39421 tcctatcagc acctataaac aaaacttcaa aggtcacatt agctttgttt ctgtaaataa
    39481 agaaaatgga gaaatgaatg ttgctttcca attaaaaacg ccaggtgtta acttcgactt
    39541 agctcgtgct ggtaaaggaa aatcacatgg ctggttcttc ttcagtactt ataatacaga
    39601 acaagcaaat acattgttag aagtaaacgc atctcaaaga gataaagact tcattatggc
    39661 tgtaaactgg aaaaaagctg aagaatatat caaagcagga aaaggcaaga aacaagctgc
    39721 aaaatatgcg cataataaat tcagcgaaga aacacatacc gctacttcag aaatgaaaaa
    39781 tgaagtgacc gtattagatg tggctgaatt ccctgatatc gtatatttta ttccatgccc
    39841 taaatcacca cacggttgcg acgttgaccc aacaggtgag tacattgtag gtagtggtaa
    39901 attagcagct ttattgccag tgtttagctt tgataaaatc caaaaagcaa ttgcggataa
    39961 acagttcgaa gctgaaaaat atgatggtat tcctgtaatt aaatacgaag ctgcattgta
    40021 tggtgaagta aaaaaaccag gcttaggacc tttgcataca gagtttgatg cgaatggtaa
    40081 tgcttatacc tctttcttcg tgtcttctga agtagtgaaa tggaatatta aagacttgaa
    40141 agtaatcgat agacagccaa cttactactc tgtgggtcac ttaatggtgc ctggtggtaa
    40201 tactaaaaaa ccttttggta aatacttggt tgcctacaac aaaattacaa aagaccgtta
    40261 tttacctact ggtcctgagt tgtctcaaag tgcacaattg tatgacatca gtggcgagaa
    40321 aatgaaatta attttagatt atcctactat tggtgaaccg cattacgcgc aagcagtgcc
    40381 tgctgatttg atttctaaaa atcaagtgaa aatctttaaa attgatgaca acaaacatcc
    40441 ttatgtgtca aaaggtgaaa aagaaactag agtaatacgc aacggtaatc gtgttgatgt
    40501 atttatgact gctattcgct ctcacttagc acctgataat attgaaggta ttaaaatggg
    40561 cgatgaagtt tatttccacg ttacaaactt agagcaagat tgggacgttc ctcatggttt
    40621 cgcaattaaa ggagcaaaca atggcgaatt attaatcatg cctggtgaaa cagtaacttt
    40681 aaaatgggta ccagacagaa caggtatttt cccaatgtat tgtacagact tctgtagtgc
    40741 tttacaccaa gaaatgcaag gatatgtacg cgtatcgcca gcaggtagca atgtgccact
    40801 gtctttccat atcggaaaaa cagagccagc accagcacca gctgcgaaat aagaaataag
    40861 tgaacatttt tttaaggagc agcgaaattt catttctctg ctccttttat ttttaatttt
    40921 aaatcagaat catggacaag gtcaatgaac aaaaaatatc caatttgtct cggatattat
    40981 tatttctttc ttcagttttt ttagtcattt ccattttcgt accgctttgg cgaattgatt
    41041 tagatgcacc gcagtatcca gaaggcttac aattattgat ttatacgtat aaacttggtg
    41101 gcaatgttga gattataaat ggacttaatc attacattgg tatgaaaact ttacatgctg
    41161 atgattttat tgaattcact gtattgccat atattattgg cgctttcgca ttatttgctt
    41221 tgattactgc actcattggt aaaaagaaat tggtgtatat tttatttata gcatttgcat
    41281 tatttggtgt actttctatg atagattttt atagatggga atatgattat ggccacaacc
    41341 tcgacccaaa tgcagcgatt gttgtgccag gcatggcata ccaaccaccg ctaatcggtt
    41401 ttaaacaatt attgaatttt ggagcttttt ctattccatc ttatggtggc tggttgttta
    41461 ttatttgtgg cgtgtttatg ttagcagcag taagcatcga ggcaggatgg atttctaaat
    41521 ttaaaaaaca aaaacctgtt gtaagcatat tgctttcgct cttatctatt gcgctactta
    41581 cttcatgcgt aagcacagaa ccgcaagcaa taaaattaaa tacagataat tgtgattatt
    41641 gtaaaatgtc aatcgccgat ggtcgatttg gcacagaagt cattacaaaa aaaggaagag
    41701 catataagtt tgatgatatc tcttgtttac atggctatat cacagaaaat aaacaagtgc
    41761 cgataggagc gatgtatgtt gcagactttg caacacctac cttgttaaca ccagtagaaa
    41821 ctgcatttta tgtaaaacat gctgatgtta aaagtcctat gggcggtaat gttgctgcat
    41881 tttctgacaa ggcgaaagca gaagaatatg caaaaaaatt aaacacaagt gtattggatt
    41941 ggaagtcctt aaatttataa attgaataga tttttctata tcgttttttt aattttttta
    42001 gtacataatg tacttggaaa aactattgtt gtagggaaag gacagccata tacaaaaata
    42061 aaaactgctg tacatgttgc atcgcctggc gattcgataa ttgtattaaa aggtgtatat
    42121 aaagaaggta atattgtcat tgataaaccg ctaagcttag tagggaaaaa tctgccaatt
    42181 cttgatggac aaaaaaagta tgaagtactt tctgttaaat caaataatgt tacagtagat
    42241 ggctttgtaa tacaacgctc aagcgtggct acattagacg atcctggagg cataaaagta
    42301 tataacagca attatgtggt gatacaaaac tgcatactag aagataattt tttcggaatt
    42361 tatattcaat atggcaagca ttgcatcatt agaaataaca aaataaaagc gtatggcatc
    42421 gaagaacaac aaatcggaaa tggtattcat tgttggaaaa gcgatagcct tttgataata
    42481 ggtaataaag tagatggaca tcgcgatggc atttattttg aatttgtgac aggctctact
    42541 atctggcgga atattgccaa caacaacatt agatatggct tgcattttat gttctctaac
    42601 gatgatgcat acatttgtaa tgtctttaaa aacaatggtg caggtgttgc cgtgatgttt
    42661 acaaaaaatg tgaaaatgta taacaattac ttttctgaaa attggggtga tgcagcgtat
    42721 ggtttattgc ttaaagaaat ttcggatagc tatatcattg gcaaccattt tgaaaataat
    42781 acaacaggca tttttatgga aggcacaagc cgtatcattg ctgaaaaaaa cagcattaag
    42841 caaaatggct ggggaatgaa aatacaagca agttgtatgg acaacaccat taagaaaaat
    42901 aactttattg ccaacacctt tgatattagt actaacggta cattggtgct caatacattc
    42961 gataataatt attgggacaa atacgaaggc tatgatttaa ataaagataa aattggagat
    43021 ttgccttacc atcctttaag cttgttttct gttatagtgg aaaaaaatcc accagccatg
    43081 ctactattta gaagctttat ggtaacactt ttagataaat cagaaaaaat tattcctagt
    43141 cttactcctg ataattttat tgataattca ccgctaatgc gaccactatt gctatgataa
    43201 acatccaaca aatatcaaaa cgtttttcca aactcgaagt gttaaaacac atcgatttgc
    43261 aatgcaacaa aggcgaatgt attgcactga ttggcccaaa tggctgcgga aaaacaacgc
    43321 tcatcaaatc tattttagga atggtaattc ctgatagtgg cgaaatcatt gtaaacggcg
    43381 aaaatacaaa agccaatttc gaatatcgta aacatattgg ctatatgccg caaattggta
    43441 ggtatccaca aaacatgacg attggccaag tcttacaaat gatgaaagaa atcaggcaaa
    43501 caacatcaac actagacgaa gatttgataa agtcttttga attggaacat ctttatggca
    43561 aagcaatggg aacactttct ggaggcacta cacaaaaggt aagcgcagct ttagcatttt
    43621 tatttaatcc tgatatttta atcttagatg aaccaacggc agggctcgat cctttggctt
    43681 cagaaatctt aaaagaaaaa attataacag aaaaaaacaa aggaaaacta attctgatta
    43741 catcacattt acttagtgaa ttagatgatt tgattacgca aattattttc atgcaagatg
    43801 gaagagtaga atttcatcat agcgtagaac agcttagaaa tgatactgga gaaacaaaaa
    43861 tatcgaaagc aattgccttt atcttaaaaa ataaaatcgg aaataaagat gtaatcacac
    43921 aatctacaat taatcaaagt gtcaagtcat gaacagaata ataaaatatg taatcatcga
    43981 tattctcaaa aataaaatag ttgtagcata tactatcatg ttggctgttt tgtcatggag
    44041 tgctttcagc ttagaagata gcagtactaa aggcatgctt accttgttaa atattatttt
    44101 actgacagtt ccgttggtgt ccatcttatt tacaactatt tatatttata acagtgcaga
    44161 gtttattgaa ttattattga gtcagccaat agctagaaaa aaaatttggt ttagtctttt
    44221 tatcggcttg tctatgtcgt ttatcgtttc atttatcatt ggtgcgggaa ttccattatt
    44281 aatttatgca ccaataaata caggcgttat gatgttgctc ttaggtgtaa taatttcagt
    44341 tgtgtttatt tccattgcct tcttaagttc tattacgaca cgcgacaagg caaaaggtat
    44401 aggaatgtcc atcatgttat ggttgtattt ttgtttgttg tttgatggta ttgtgttgtt
    44461 cttattattt caattttctg attatccaat tgagaaaata atggtcatta ttacagcact
    44521 cagtcctgtc gatttagcaa gaattttgat attacttcat ctagatgtct cagcaatgct
    44581 aggttatact ggtgccattt ttaaagattt ctttggcact actttaggat tccttatcag
    44641 tttgtgtttg ctctgtttgt ggtttattgt tccctattgg atttcattag caagatttag
    44701 aaaaaaagat ttataataat tgagcagctt ttataaaata taaaagtgaa ataaaatgaa
    44761 aaaagacata aaaaatagaa aagatatcga aaaattggtt aatgcgtttt atgcaaaaat
    44821 caaagaagat aaactattgc aatctttctt cacgaaccct gaagggttta attgggaaaa
    44881 gcacttaaat acgatgtata atttttggga aaatatgcta ttcttctctg gaaattatga
    44941 aggtaatcca cttcatttac atcgacattt acatcaattg acaaatatta aaaaatcaca
    45001 tttccaacgc tggaataagt tgtttgcaga tacagttgat gaattatatg caggcaaaaa
    45061 tgcagattta attaaaaaac gttccatcaa tatatccaac atcattcaaa aaaatatcat
    45121 accaaaagag atgtcagaat gatgtttgaa tataatgttg attttttcat cgagtaataa
    45181 aaacaagaga caatagaaaa taatttttaa cataaataca taaaaaaaca aattatgaac
    45241 ggaggcgaaa tcattgcaaa agttttacaa aatcatggcg taaaatttct ttttacactt
    45301 tgtggaggac atatttctcc catctttgtt agtgcacaaa aaataggcat tcgcgttgtt
    45361 gatactagac acgaagtaaa tgcagttttt gcagcagatg cggtatctcg attgactggc
    45421 gtgcctggag tagcagctgt tacagcagga ccagggttaa ccaatacgat tactgcagtt
    45481 aaaaatgcat acatggctca atcgccatta atcgtattag gtggtggtgc agcaacgatt
    45541 ttaaaaggta gaggctcatt acaagatatc gaccaaatga gcttgatgaa accagtagta
    45601 aaatgggctg ctcgtatcac aaaagtaaaa gaaattgctc ctacattaga gcgtgcattt
    45661 gcagtagcac aagaaggcat tccaggtcct gtatttgttg agtgtccaat cgatacttta
    45721 tataacgaag aattggtgcg cgaatggtat ggcgcaaaaa gcaaagacgt gccaccacgt
    45781 aatatgcaag agcgtgtcat ccaatggtat gtaaatagac atgctcgtct cttatttgaa
    45841 gataaagaca aaattcgttt tcacgaacca caatcgttta caattccaaa tcatcaatct
    45901 tctgacattg ctaaagcggt agctatgcta aaagaagcga aaaatccatt gatgattatt
    45961 ggtagcggtg cattaatgca acctaataaa gcagatgaat tagccgcagc agtagtaaaa
    46021 ctaggcattc cagtgtattt aagtggtatg gcgagaggtt tgttaggcaa agatgccaac
    46081 ttgcaaatgc gccataaacg taaagaggca atcaaagaag cagacttaat tttgttagct
    46141 ggcgtgccta atgattttag attagactat ggaaatcaca taggacatcg tccttttatt
    46201 tcaatcaata gaagcacaga agatttatac aaaaataaaa ggccaacaat tggaatacat
    46261 gccgatcctc aagattttat tatagaatta gctaagaatt atgcaatcaa taatccagaa
    46321 tggataagca aacttcagaa aagagatgaa gaacgtgaac gcaatattga tgcgcaagcg
    46381 aaagataaat tgaatggcat aaatccagta caactgttca gatcactaga tacgttatta
    46441 agtgataata ctattttagt tgctgatggc ggcgactttg tggcaacatc agcatatact
    46501 ctaaaagcga gatcgccact ttcatggtta gatccaggcg tatttggaac gctcggcgtt
    46561 ggtgctggtt tcgcattagg tgcaaagtta gtttatccag aaaaagatgt ttggattatt
    46621 tatggcgatg gaagcgctgg ttatagctta atggaatatg atacattcgt tcgacataat
    46681 ttgccagtga tttcacttat tggaaatgat gcttgttggg cacagatagc tcgtgaccaa
    46741 gtagatttct tagaaagcga ttgcgctgta acattagcac attccaacta tgaaatgata
    46801 ggcaaagcat ttggtgcagc tggcgatcgc gtagaaacga tggaagcatt taatgaatct
    46861 gtgttggctg cattaaaaaa cactagaaaa ggtatgcctt atattattaa tgctattatc
    46921 ggcaaaagcg aatttagaaa aggttctatt tcaatgtaat tgctatgaaa aatatcggat
    46981 taataaacaa agaaacaact gcattagtag tcattgatat tcaagacaaa ttaatgcctg
    47041 taatgcaaca agcggaactc atatttgcca acgcaaaccg cttgttaaaa ggtgcatcaa
    47101 ttctgcaaat tccaagctac atcacagagc aatatccagc aggtttagga aatacttgca
    47161 cagcgataga gatgccttct gatattactt tagtagaaaa aattgcattc agttgtatgc
    47221 agtcacagca attattagaa agctggaaga aaaacaatat caatacattg attctatgtg
    47281 gtgttgaatc gcatatctgt gtgctaaaaa cagcattaga cgcactcgct gcgggctttg
    47341 atgttcatgt agttgcagat gcggtttcct ctcgaactgc cgagaataaa aatttggcac
    47401 tcgagagaat gcgtcagtct ggtgctttta ttgtatctac agaaatgttg ttatttcaat
    47461 taattcaaga agcaggcaca ccagcattta aagcgattag caaattaata aaataaaata
    47521 ttcatacata acctgactaa aatggaattt aaacgcttat tcgacttatt gaccaatcat
    47581 gtcaatgaaa atccaaataa cttattcctt gcttcaaaag taaataataa atggaagcat
    47641 tattcatttc aagaagtgca acaagtagcc aataaagtaa gccaattgct attatcattg
    47701 aacttgcaga aagatgataa aattgctgtg attgcgaaca atagacccga atggaatttt
    47761 acagattttg gtgcacaaca aattggcgtt actgtcgtgc cgatgtatcc aactatttca
    47821 gagcatgatt atgaatatat ttttaatgat tcagaaatta aatatgcttt tgttggcgat
    47881 aataccatat tacaaaaggt aaaaacactg ctgcaaaaat gcacttcatt aaaaggaatt
    47941 tatacttttg atgaaaccga agatgccaaa tcattttggt ctgcattgcc tgaaaatatt
    48001 gatattacag caatcgaaaa tattaaagca acagtgacag aagatgactt ggctaccatt
    48061 atctacactt ctggcactac aggaaatccg aaaggagtga tgctcacgca taaaaatatt
    48121 gtgtcaaatg taatcagtgt gaaagaggtt acgccgtttg tgataggaga aaaatctttg
    48181 agcttcttgc ctttgtgtca tagctttgag agaatggtgt tttatgccta tttatgtttt
    48241 ggaattcata ttcattatgc cgagagcttg gaaactattg cagataattt gcaagaagta
    48301 aagccatatt gttttaccac agttcctcga ttattggaaa aagtatatga gaaaattatg
    48361 gcgaaaggtg cggcattaac gggcgctaag aagttattgt tcgattggtc gatggaatta
    48421 ggcgctcaat acgaaatagg cacaaacaaa ggctggtgtt ataatacaca attggcaata
    48481 gcaaggaaat tagtattcag caaatggcaa gaagccttag gcggaaatgt tcattttatc
    48541 atcacgggtg cggcagcaat gcaaccacga ttaattacgc tgtttactgc agctggcgta
    48601 aatgtattag aaggctatgg tcttacagaa acatcgccag ttttaagttg taatttagtc
    48661 gatgaaaaac aacgttgtat tggcactgtt ggtattccaa ttcctggcgt ggaaattaaa
    48721 atggcagaag atggtgaaat tattgccaaa ggtccaaata ttatgaaagg ctattataaa
    48781 cgacctgatt taacagcaga agtaatcgat aaagatggtt ggtttcatac aggcgacatt
    48841 ggaacctgga tagaaaaaaa tggcaataaa tttttaaaaa ttactgacag gaaaaaagag
    48901 ctttttaaaa cagctggagg aaaatatatt gcaccacaag tgatagaaaa taaaatgaaa
    48961 gaaagcccat atatcgaaca gataatggta gttggtggcg atgataaaaa atatatctcg
    49021 gcattgatag tgccatcttt tgtgcagcta acagcatggg ctgagaaaaa tggtgtagtt
    49081 gtattgtcaa atgaagagtt gataaaagac aaacaagttt tatcattatt agataaagaa
    49141 ataaaaaaat acaatgataa ttttggccaa tgggaacaga ttaaaaagtt tgtcgcactg
    49201 ccaaatgaat ggtcagtaga aagtggagaa ctgacgccta caatgaagcc aaaacgaaaa
    49261 attatagcgg agcgttatag caaagaaata aattcgattt acgcagcaga ctaaaagaat
    49321 atgcgccttt gaagaattca aaggcgcatc aattaaaccc atttattatt tcaaaagatt
    49381 attttgtcgg tggaagcaat acctcattaa ccacatgtat catgccattt gaagtaggta
    49441 tagatgcaac aatttccgca gtgccattca cataagtttt tccgtctttg acagatattg
    49501 tcaccttttg tgcgttcact tgttcaaaag attgtccatc ttggaaatac tctgctttta
    49561 aagagccaac ataagtatga tactctaaga tgttttttaa atcttctttt ttctcaggtt
    49621 ttaaaagctc ttcaacagtt cctttcggca acttttcgaa agcagcattc gttggcgcaa
    49681 atacggtgaa tggaccagca ttacttaatg cgtcaaccaa ttctgctgct ttaacagcag
    49741 ccactaaagt gctgtggtct ttgctgccag cagccacctg aacgatgtta ggattggata
    49801 catcatcttg cacacctgat tgtccgacag caggagaaga ctcttcactt gcagcactag
    49861 catcgcctgc tgcttcattg ttcttgcatg cagtaaatag taacattgta actgcaaata
    49921 gcattaaaag ataatttttc atttttgttg aatttggtta aaaattacat tacaatttta
    49981 agccaaaagg agcgaagttg agatgcgtaa tgtcatgctt tataacgttt tattatgata
    50041 gatgtcataa aatgcatact taaataattt cctatcgttt ttctaaaaaa ccaatgattt
    50101 attatttcaa tataatataa agcatacgaa aatatttgcg ttctttttac aaaattttaa
    50161 ttgtaagaca gagcgcgtta attaggtttt tcatggatta gcttaaatac gagtgatttt
    50221 tatatttttc ttggaatttc attatagatg tcgtttttat tattttacgc ttaaaattaa
    50281 taatatctaa aatctaaaaa aaacttcact ttttgtgaac tatttttaat atctttgcat
    50341 ttatacaatg atagtcagac taattaagaa gaaaactatt gaagattttg ctttacaaaa
    50401 tgccaatagc agaagttctt tcaagttgtg gttatctatc attaaactgg ctgattggga
    50461 aactccagat gatataacag caacttttgg ttctgcagat ttattaggaa atgggagtaa
    50521 tagagttgtt tttgatatag gaggtaataa tttcagaatg atttgtaaat accatttttc
    50581 caagacatta gtgcatttat atatcaaatg gataggttct catagtgaat atgataaact
    50641 ttgtgataag aataaacaat atactataga catcttttaa ttattaaaca tgagcacatt
    50701 acaatataaa atcatcaaaa ctgaaacaca gtataaaaag tattgtgata ttcttgaatc
    50761 attggttgat agtggtaaaa acactaaagc tgttcaagat gaaatagaat tgctatcatt
    50821 attaattgaa aagtatgatg aagagcataa ctcatttgat gaccttgacc ccattcagtt
    50881 attaaaagga ttgatgaaag agcataaaat gaaagctgtt gatttatcaa atcttttaaa
    50941 tgtaagtgat ggcttggttt ctgatatttt gaattacaag aaaggactat ctaaagaaac
    51001 aattagagtt cttgctgaca ggtttaaact taatcaaaaa gcatttaata gaccttatag
    51061 tttgaaaatt gcaactacaa agaaagagtt agcttttgct taaagggtta caattttcct
    51121 cgcaattatt ggtgtttagt ttacgaagct aatgaggagt atatgccatc aataactttt
    51181 atactacgca aaaagaaaac aaaaaagcct cgcattgctg cgaagctttt tcttggttag
    51241 tcgggatggc tggattcgaa ccaacgacct cctggtccca aaccaggcat tctaaccgga
    51301 ctgaactaca tcccgattgg ttggattgtt tgctgcggtg agggagggat tcgaaccctc
    51361 ggtacagttt cccgtacgtc agtttagcaa actggtggtt taagccactc acccacctca
    51421 cctccttatt tctttacaag aactttggga cggcaaagat aggtttttta aagaactttg
    51481 caaatatact gcaaaaaaaa tctgcattta ttgctttaat aaattgataa tgtttttttc
    51541 aaaaaaaaat cctgcttagc aggatccttt ttttctataa acttgatttt ataaactcaa
    51601 ttcaattcgt tttttcaatt cttctactga gtctctaaat tcttcatctg tatcgataag
    51661 attgcgcacg gcttggcaag aatgtataac ggtgctgtgg tcgcggccgc caaattgatg
    51721 cccgattgct ttcaatgatg acttggtata ttcttttgca aaaaacatag ataattggcg
    51781 tgcttgcaca aactgtcttt tacgggttgg ttcttttagt ttttctacag gcacttcaaa
    51841 gaaatcgcat accgtttttt gtatcatgtc taatgaaatt tctttcgttg catgttttac
    51901 aaagttgatg actgttttct tcgctaagtc taatgtaata tcttgtctat tgaaagtagc
    51961 ttgtgccaaa atagacgtaa gcacaccttc caattcgcga atatttgttt tgatgtttgt
    52021 tgcaataaat cgtgttacat cttcattcat ctcgatgcca tcagcatgta atttcttttg
    52081 taagatgcta atgcgtgtat cgtagtctgg tgtttgtaag tcagtagtca agccccattt
    52141 aaaacgagac aagagacgtt cttccatatt ttctaaatct tttggcgcac ggtctgatgt
    52201 taatatcagc tgttttccat tttgatgtaa gtgattaaaa atatggaaga aaatatcttg
    52261 tgtttttgtt ttgttggcga agaactgaat atcgtcaata attaaaatat ctatttgctg
    52321 atagaaatga ataaaatcat ttgtgttatt atcttttata gcatttaaaa attggctagt
    52381 aaatttctct gagcttacat ataaaacagt cttagttgag ttattgactt ttatataatt
    52441 gccaatcgct tgtgcaagat gtgttttacc taagccaacg ccaccatata ttactaaagg
    52501 attaaaggct gtgccaccag gcgatttggc aactgccata cctgcgcttc ttgcaaattt
    52561 attacaatca ccttctataa atgaatcaaa agtatagttt ggattgagtt gcgagtcaac
    52621 atgtatcttc ttaaggccag gaattacaaa tggatttttt accattggac tcgagaagat
    52681 tgttggcata gatacttcgt ttgtgccata cacaccttct ctatgattgg gcacatcgat
    52741 agtcttaggt gcagttttgg tgccattaga ggtatccatt aagatgcgat attctagttt
    52801 agcatcatca cctaattctc tcttaattgt tttgcgaagc aaagtgacat aatgttcttc
    52861 tagccattcg taaaaaaact ggcttggtac ttgtattgtt aaactgttag catcgagttt
    52921 tacaggtttt ataggttcga accaggtttt aaagctttgt acattaatgt tatctttgat
    52981 aattttgaga caattttccc acacaagttc atgcttgttt ttcatacgct ctattaggtt
    53041 ttttaaaaat taaaaatgga taactttgat gaatctaagt tgagaaaaat ttccttaata
    53101 aaaaaattat tttcctcttg tttttttatc aacaattaca gtttgagtgt ttgataactt
    53161 tttattgaat tttttttcga aaagataatc tagctgtccg atttgaatac cagcccaacc
    53221 cacttgatta atatatatcg ttttgccgtc tttatttttt tgacttactg gtgcatctaa
    53281 aaaggtatgt gtatgtccgc caatgataac atctatatga tttgtttgtt gtgctaaaat
    53341 aatatcagat atttttgttg attgatattg atagcctaaa tgtgataggc aaataatcaa
    53401 atggcatttt tcattatgtt ttaaaatatc tacctgttct tgcacacatg aaattggatc
    53461 taaatactta gtagaagtat ataatttatc tggcactaag ccgtcgagct caacaccgca
    53521 acctgtaatg ccgattttta atttgccttt tttgatgata atatgcttgt gcactttgcc
    53581 atgtacaggc gtgccggtaa aatcataatt gcaatttaca aatgggaaat ttgctctagt
    53641 aatttgtttt gcaagaccat caataccacc atcgaaatca tgattgccga ttgtggcagc
    53701 atcatagccc attttattca tcagatcgat ttcgatatca ccattaaaaa aattaaaata
    53761 gggtgttcct tgaaagatat cgccagcatc caataggagt acgtgttcct tgttttttcg
    53821 tatttcatgt acgatagcgg ctcgctgcgc tgcgccacct aatccaggat attttttatc
    53881 gtcatcagca aatggttcta tgtgactgtg cacatcgtta gtatgtaaaa cagtgagtgc
    53941 actttttgta ttatcagcca ataaggaaag cggcgagcct acggcagtaa gcatggcacc
    54001 agaaagtaca ctttgtttga taaatgttct tctattctgc atattgaatt ctattttcca
    54061 gattagattg aattttctta cctgctttag tttgctcttt ccagtattct atgatggcat
    54121 ctctatataa tttcccagtt tgaatttgcg gtattgtttt taaaaatgtc attccatcgc
    54181 caccatttgc caagtagtca tttgtggcaa cgttgtaatt ttttttcaaa tcaattggaa
    54241 tgccgtttac gcgaatgtca cttgcttttt tatttttgat aacaaaggca ataccagcaa
    54301 caggccatcc gcctttgata gccaaagagt cgcaataatc gctgagttgc tgtcctgtta
    54361 aaatttgttc taccaaataa ttatcaaatg gcattagcaa ataagcatgc tctaccatta
    54421 atgtaccttt gctgagtgat ggtgtgcgta tgccaccgta atttaaaata gcaacgtcaa
    54481 ttgtttgatt cgtatatgca gccgttttta attttaatat atcagccatc atatttccca
    54541 atgtgctttc aggcattttt ttagaaagat cttccgcaag ttctgccaat ggaattttca
    54601 ttattttgtc taaagaatct ttgtaaggca agtaataggc gagcgttgca ctatcggcaa
    54661 ttatggtttt atttatttta tttagactaa atttttcttg tattggaacg taggttttgc
    54721 atgctgtaaa aactacaatt gcaagcatcc aaaatgcgat atatttagaa taatttttta
    54781 acatcacttt ttacataatt aatggcaatt tgtacaggca catctttatc gctttgcata
    54841 taataattaa agattgcttt gtttaattca atgccgtctg cgtcatcagg taatttagaa
    54901 gcaaataaat ttatcacttt tattgtttct tcttttgctg ttgacaccat ttgccacgca
    54961 tcatcattga tatacatttg ttgcgataag ttatattcat attcgatgcg tatgcttttg
    55021 attaattcta actgtaattc tgccaccaac atattcggtt gatatactct ggtgagcaag
    55081 ctaaatggat gaattctttc tagtaaaaca agaatgcgct cataagcttg caagcgcaat
    55141 ggcaaagaca ctttgcgttg ttcaaataac agagatttat ttatttcttg atttcttcgt
    55201 ttgttttcat tttctataaa tactttgata attgaataaa caccgccaaa cacgactaat
    55261 gaaggaacag tatatttaag aatttctaaa atggtagtaa gcatgtgttt gttgttttcg
    55321 aattgatgtt aatgttatgt aaaataggaa tctaaaaata caaattttat tgcaatctat
    55381 gtttgtgaat ttactttcct tgttaaaacg aataataaat gtatttattg aactaacttt
    55441 gcttaatatt gttaaatagc tatgatgatt gatactgtaa ataaattacc aatttcgctg
    55501 acgcagaatg cggtacaaga aatattgcac ttgtataaaa atggaaacta cgaagccgga
    55561 tttggattgc gcattggcgt gaaaggtggt ggttgtgccg gattttctta tgtattggaa
    55621 tttgatgcac agaaagaaaa tgacaatgtg tattccatta attcaattcc tttatatgta
    55681 gataagtcgc aagaacttta tttacttgat tgtgaaattg actttaaagg tggtttggat
    55741 aacagaggtt ttatttttaa taatccaaat gctacatcaa cttgtggttg tggcacctct
    55801 ttttcggcat aaagtcagta atagcaagca ctttgaggca taattttaca caagcctcca
    55861 tttttactaa cattttcgct taattatttg gataattaaa taaacaattt aactttgtgt
    55921 cataatgttt ttttaaccat taaataagaa aataatgaac aaaggagatt tagtaaaagt
    55981 aatcgctgat gcagcaggtg taacaaatgc agcagctaat gacacattga acgctgtatt
    56041 agacagcatt cgtaaaacgt tgaaaaaagg cgacaaagtt acgttaattg gttttggtac
    56101 tttcagcact tctaagagag aaaaaagaga aggaagaaat ccagcaactg gtgctaaaat
    56161 taaaattcct gcaaaaacaa tcgtgcgttt caaagcaggt aaagaattat ctgaagcagt
    56221 gaaaaaaaat aaataatcaa actgctaagt aataaaataa gcctccatgt gaggcttatt
    56281 tttttttcta aaagtaagta caaaacatta aggagagagt cgctcgattt tccaatcttg
    56341 atttgcttct aaagaatatt gaattctatc atgtaatctg ctcattctgc cttgccaaaa
    56401 ttcaaacaat gtaggcacca ccaaatagcc gccccaataa tcaggcaatg gcactacttt
    56461 attagcgtat gctttgtagg ctgtatcata tttttcatct aaaatttctc gactctcaat
    56521 tatgctactt tgcaaggaaa cggtagcacc tatttggctt tcaattggtc gagaatgaaa
    56581 gtattgcatg gcactttctc tatctaattt ttgtacagct ccttcaattc gaatttgtct
    56641 ttgttgccac caaaataata aggctacctt ttcattttct tgtatttgct ttcctttatg
    56701 gctttcataa ttgctataaa atacaaatcc atttttgtcg aaacttttaa gcaacacgat
    56761 tcgagcagat ggctgaccgt catggccaac cgttgccagt gtcatggcat taggttctat
    56821 aatattttgt tgaattgcat cttgaaacca agcatcaaat tgttgaaatg gattggtatg
    56881 gcagtttgct tctaataaga tatcagcgtc atactgctca cgcatattgt ttaaatctcg
    56941 ttgtggcata actgaaaatt agctattata tttgaaacac agaaaccaat tttttgcaaa
    57001 taatgaaaaa taacaatata gacaatcaag catcacaaaa ttttaaaaga gcagccttgc
    57061 ctttaattat tttcaccaat ttatttccaa tatatggtgt gataacatac aattggacaa
    57121 ttttttccat tgtctatttg tattggttag aattattaat tgtcagcaca ttccaattgc
    57181 tgaaaatatt gacagccgaa ggtgataaag aaatcacaat gattggcaaa ctgtttcttg
    57241 ccattcgttt tttcttattt cgaactggta ttttcctttt ctatttactt tttatagtta
    57301 ctttcttagg attgctaatt acgaacaaag aaaattcaga tagtgaatta ttgactttcg
    57361 cacatgcatt gacgctgaaa ggtagttttt acaggattac tttaactagt ttttttattt
    57421 ataatttggt agaatatctg gtgcaattta ggttgaatgg taaatataag acatcaacgc
    57481 cgaatgataa ttttacgcta ctcgacccac atattattgt cgtgcatatt gtagttgtat
    57541 taggtacttt tctctatgca ggtgtcaccg aaaaattatc attagaccat cacacagcta
    57601 tcattgcctg tgccatatta tttgctatcg taaaaataat agcagatgtt gtcaggtatc
    57661 aaatgtctga caaatctgct actatttcgt cggaaaaata tatctaaatt tatagatttt
    57721 ttactaagtc ggctttttcg atatagcctt tatgtaccca agtttctttc ccttttacaa
    57781 aggtttttaa aattggaatt tctgcaattt ttaattgctg tgcgagttgt ttattttcat
    57841 taatatctaa tcgaataact tttacttttc cggcatattc tttttcaatt tcttccaaca
    57901 ttggcttcat cttcatgcat ggcgcacacc atggcgcaaa ataatctact aatactagcg
    57961 tgtctgatgt tatcatttgc tgatacattt ccatagtgat tttatctgct gctgctttgc
    58021 tgttttctgt cacgattggc aagttggcag attgccatgc taagatgccg ccgtcaagat
    58081 taacgacatt agcaaagctg tttttgcgca ttacacttgc cgctgacgca ctgcgtttgc
    58141 ctgacagaca atacacgaag taggttttat tcttatcgag ttttgaaatt tcttcttcaa
    58201 aagcggcact attgtagtcg atgttttgtg cgttttttat aaagccacca gcatattctt
    58261 ctggtgttcg aacgtctaaa atttcagcat cacttgtcgt atttattttt tcgttaaatg
    58321 cagtaggtgt caatacttgt ccagcatttt gtgagttgca cgatgtagaa ataaaagaga
    58381 ttgttagtaa tagtgctata aaatgtagtt tcatttttta ttgtttaatg gtgatagtag
    58441 gattgaaaac gatggtagat tttattgagt ttgaaattaa acaggctctt tctgatttga
    58501 ttaaaacacg ctctgctttt tctttatctt gttcattggc aatcgtaatt tctggttgta
    58561 gttccacttc actcatcatg tattttccat ctatcatttc cagttttccg atcgcttttg
    58621 agtcaaaact aagatactct aattttgagt tctctgcaat agctaaaaat gtggtcatca
    58681 agcaactgtt
//
LOCUS       DAOQQV010000003        55015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_158_length_55015_cov_7.538246, whole genome shotgun sequence.
ACCESSION   DAOQQV010000003 DAOQQV010000000
VERSION     DAOQQV010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 55015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55015
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_55015_cov_7.538246"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLL59_00670"
     CDS             <1..1373
                     /locus_tag="PLL59_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMY22340.1"
                     /translation="LRKAMGKKDRAKLDSLKPMFIDGCKAKNLDEKICEKIWADWEKF
                     ASYAFNKSHSTCYAFVAFQTAYLKAHYPAEYMAAVLSHNMSDIKQINFFLSECRRMQV
                     ITLGPDINESDVKFSVNKAGEIRFALSAIKGVGEAAVTDILLERDKNGAFKNIFDLTK
                     RVNSRTVNKKNIESLAQAGAFDSFPNVHRAQYFYKYKDESSFIELAIRYGNNVKDQNA
                     SAAFSLFGGTSDAEIPEPEIPQTHPWQSFEKLQKEKEVTGIYISGHPLDDFELEIKTF
                     CNATFEIIENFRESEVTIACMVSSVNKRIAKNGKPWANVLFEDLDSTMEIAFFNENYT
                     KYLGFLEQGNRLFIKGIYKQSFRDQNKYELRITQIDFLQDVLERYGKGLRILMHYKDM
                     NEAKVDKIYNIVSKYKGKKPFSFYLYDDNEQPLTFQSTKVGVKLENELINQLKYIPNI
                     VVKLLS"
     gene            1477..2283
                     /locus_tag="PLL59_00675"
     CDS             1477..2283
                     /locus_tag="PLL59_00675"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HMY22341.1"
                     /translation="MKKIFTLAFSIIISSTLFASGEFFIKLNSNGNYTVSLNNQIITN
                     TGSVFRFFDLYSGNYNLKVYENGFNGRTIFEQTVNVLDGYRIVAELDRYTGLKIIEKI
                     PFTQSSWYVDHLQNYNNNWYPNNPKPSRPKCGNNWNHNWNNQQGWNNNWNNGWGNGSN
                     NNYPNYPGNYGYGNVLEDNELQSLLSVIKNSAFDDKKIEIIKSALKNRMLKTDHVSQL
                     MQQFSFEQNKLTIAKYCYDKTMDKNNYYTLYDDFAFSNSSSQLAKYIESK"
     gene            2465..3772
                     /locus_tag="PLL59_00680"
     CDS             2465..3772
                     /locus_tag="PLL59_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMY22342.1"
                     /translation="MQKFATIICLFILVNTFAQPLYFPPKNNSTWDTISPRTLGWCND
                     DIDSLYTYLGNANSDAFIYLKNGKIVLEKYFGSFTADSIHYWASAGKSLTAMMIGIAQ
                     QKGKLNINDTVSKYLGTSWTSESLEKENKITIKHLLTMTSGLDDNPTQPCDNLSQTAT
                     CLQYKADAGTRWAYHSGAYRKLEDVITAANGTNYNTASYDWLESKTGMNGFWFNSVYY
                     SNARDMARYGLLTLNRGIWQADTVLNDTSYFRAMINTSQNKNLSYGYLWWLNGKNSVM
                     LPGTQTVFPTKLIPNAPDDLYCALGKNDQKIYVIPSTKTVIVRFGDSNFGNDASSPFD
                     TMLWNYINRLDNSCLTTAVIQQNKSNTVIKLFPNPANNFISFSGFVSNNNIHYAIANM
                     LGEIVKEDVTLNHSIDISDLPSGQYYMLLNDDMQQASKQFIKL"
     gene            complement(3847..4986)
                     /locus_tag="PLL59_00685"
     CDS             complement(3847..4986)
                     /locus_tag="PLL59_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMY22343.1"
                     /translation="MEFNRIQQEALEKYIKPQKVTLLLGARRVGKSILIEQFLSQYKK
                     PYLFLNGEDETTLELLAVKSVKNYTRLLQGNDLLVIDEAQNIPDIGKKLKLMVDNIKG
                     LKVIVSGSSAFDLLNLSGEPLTGRSYTLNLYPLHQSEIAQQENAIQTKQNLEERLIYG
                     AYPELWHLDSLNEKRNYLNSLVHSYLLKDILVLDGIRNASILHNLLKLIAYQVGKEVS
                     YNELGNALGVSKNTVEKYLHLLTKVFVLYKVGAYSNNLRKEISKSQRWYFLDNGIRNA
                     VIGDFNPLALRRDDEKGALWENYIFSERLKHTSYSQKFVNFYFWRTYDQQEIDLIEED
                     NKKISAFEFKYSDKKKPKVPIAFAQAYPKAPFSVVNSSNYLDYIL"
     gene            complement(5091..5741)
                     /locus_tag="PLL59_00690"
     CDS             complement(5091..5741)
                     /locus_tag="PLL59_00690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMY22344.1"
                     /translation="MKQLLLYFLLMFYSVSSVKAQTYKAKIITNYGTMTVVLFDDTPK
                     HRDNFIKLVKDKFYDSLLFHRVIKGFVIQGGDPNSKHAADTVFLGEGDLGYTIPAEFR
                     TSHFHTKGMLAQARDNNPEKASSACQFYIVQGKIADDSTFIKAKQRTGNDTPESNKIV
                     YRTIGGIPHLDMNYTIYGEVVKGLKVLDKIASVKTDKNDRPLLPVRIKTIKLRPVN"
     gene            5791..5997
                     /locus_tag="PLL59_00695"
     CDS             5791..5997
                     /locus_tag="PLL59_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HMY22345.1"
                     /translation="METFEIKDDIILLNQLLKAIAWCENGAHANAVIEEGLVKVNGKI
                     ETRKRNQLTRGTIVSFDGNTVKIV"
     gene            complement(6016..8028)
                     /locus_tag="PLL59_00700"
     CDS             complement(6016..8028)
                     /locus_tag="PLL59_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMY22346.1"
                     /translation="MEHQHKYDNQGKQLCCNQVEKIYDNAGAKSLLHHSHDEHGHSHD
                     HDGHTSNIRLFLPTVLSLLLLLCAICFDHFLQASWFVGYIRIAWFFIAYLLVGFSVLK
                     EAFESIVKGDIFSEFLLMGIATIGAFAIGEFEEGVTVMLFYSIGEIFQTIAVKRAKLN
                     IKTLIDQRPDTANILVNNQIQSLKAEDVNIGDIVQLKPGEKLALDGELLSDNASFNTA
                     ALTGESKPDTKVKGDVVLAGMINLHTIAQVKITTSYTDSKLSKIINMIQDATTHKAPT
                     ELFIRKFARIYTPIVVLLAVMICFLPAIFINNYQFKDWLYRSLIFLVISCPCALVISI
                     PLGYFGGIGAASRNGILFKGSNFLDKLASIQHVVMDKTGTLTKGVFKVQEVQISPAFN
                     KQEILDLVNAIESHSTHPVASAIHQFVGEINSTIQLQQVEEISGHGLKALHNNKMLLV
                     GNFKLLNKFNIAHTVDPNAITDTVIAIALDNLFVGFITIADEIKDDAQQAIKRLHQMQ
                     VQITMLSGDKSTVVQRVASSLQIKNAFGDLLPEDKVNKVKEIKSKNESVAFVGDGVND
                     APVVALSDVGIAMGGLGSDATIETADVVIQNDEPSKIATAIQIGKQTKKIVWQNISLA
                     FAVKLIVLLLGAGGVATMWEAVFADVGVALLAIVNAVRIQQMKFDK"
     gene            complement(8040..8393)
                     /locus_tag="PLL59_00705"
     CDS             complement(8040..8393)
                     /locus_tag="PLL59_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22347.1"
                     /translation="MKQYFLTLLITISLLLNVQYSIAQSTVTSASIKTNIYCDHCLEC
                     PTCGILLLNKLKQIKGIRQVNINTTLKLIKVSYEPSVIKINSIREHIAALGYDADAIK
                     ASISAYNSLDACCKK"
     gene            complement(8413..9708)
                     /locus_tag="PLL59_00710"
     CDS             complement(8413..9708)
                     /locus_tag="PLL59_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMY22348.1"
                     /translation="MKFIIKSKNIHCLIFALFFALISCNTHHEDDGHTHANSASSQGE
                     NNNVNANSIETEPTITALTDEQINSVGIIFGKVEQKQLTATLKANGALRVPNNNKANA
                     TSLYGGVIKSIHVQVGSFVKSGQVIATIVNPQFIQLQEEYLTVSSKIQYAEQELSRQK
                     LLNDGNAGALKNYQSANAELKILNTRKASLQEQIQLMGINPATVNNTNLRTALVVKSP
                     ISGTISNVFSKIGSYVDVSSPVVEIVDNTQLHLDLNIFEKDLPILKVGQVIHFTLTNN
                     PIHEYDAKIYSIGSSFENDSKTIPIHCTVQGNKTGLIDGMNITAIVSLNNVTTNAVPN
                     DAIVDANGKSYIFMITSKKPDEHHDEDEHSHAASEIKKSSDKNTTNFERIEVVKGVSN
                     VGYTAITPISNLPSNAQIITKGAFFVNAKLTNQGEAHSH"
     gene            complement(9714..14072)
                     /locus_tag="PLL59_00715"
     CDS             complement(9714..14072)
                     /locus_tag="PLL59_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMY22349.1"
                     /translation="MLDKIIYFSIKNKFVVAVMTMLLIVWGIWSASKLPIDAVPDITN
                     NQVQIITLCPTLAGQEVEQLVTFPIEQSIANLPDIIESRSISRFGLSVITVVFDEDVD
                     IYFARQLINERLKEAEEKIPNGIGKPELAPVSTGLGEIYQYVLHPKKGSEQKYSTMDL
                     RTMQDWIVARQLNGTKGVAEVNSFGGKLKQYEVSINPFRLQAMGITIPDVFNALEKNN
                     QNTGGAYIDKKPNAYFIRGIGLVTSLEDVENIVVKSNANSVPVFIKDVAKVQFGNAIR
                     YGALTYNGEVDAVGGVVMMLKGENSNEVVQRIKEKMIVIQKALPADVIIEAYLDRTDL
                     IKRTIATVEKNLIEGALIVIFVLVLFLGNFRAGLIVASAIPLSMLFALSLMKLFGISA
                     NLMSLGAIDFGLIVDGAVIIVEATLHHLGLRQSMRKLTAAEMDEEVYVSASKIRSSAA
                     FGEIIIMMVYIPILSLVGIEGKMFKPMAITVGFAILGALILSLTYIPMMCTLFLPKQA
                     MPQHTFSDKMMAFFHKLYLPLLQKAIRFKYLLIGCTTTLFFISLFLFSRMGGEFIPTL
                     QEGDFAFHCMLPQGTSLSQSLETSMQASRIIKQFDEVKMVIGKTGTAEVPTDPMPPEV
                     TDMIIILKPQSEWKQEKSYDELADEIYEKLDAIPGVFFEKSQPIQMRFNELMTGIRQD
                     VAVKVFGENMDTLLAYANKVGSVIQTVEGTTAPMVERVEGLPQINIEYDRMRLANYGL
                     SVEEVNDIVSTAFAGKSTGVVYENERKFDLVVRLDSTFRNSIEDVSSLFIPTSQGNQI
                     PLSQVANISYKLGAAQISREDGKRRVVVGFNVDGRDVQSVVKEIQEKLYNKFHLPTGY
                     YLTFGGTFENLQQATNRLLIAVPASLFLVFVLLFFTFNSIKQALLIFTAIPMSAIGGV
                     FALLIRDMPFSISAGIGFIALFGVAVLNGIVLISTFNQLEKSGDNDVIHRILEGTKIR
                     LRPVLMTATVASLGFLPMALSNSSGAEVQKPLATVVIGGLISATFLTLFVLPLLYIIV
                     NGKFSKINSNMKTVVSSLVLMLLSAHISHAQTKVMTVDEITAMAIQNSYKLKAEDLNI
                     QSANSLIRSANEMPKLNVNVQFGQYSSIKFDNAFSITQTIPFPTLFAAKRNAIKAAVS
                     SKELDKQIAKNEIVLKVQSYFYQLLYLENYNQKLQYLDSLYQDFVATSSLRYKSGETK
                     QLEKTTAEAKRNEIMLQLSQNEFEIAICYKTLKLYMDNNDSFAIHRDAMYTPIIVSAL
                     ANTVQMEQHPTIQKLQQEAIVVEQNRKIEKANGLPDFTLSYANQSLIGFQNINNQDVF
                     FNAAKRFHYGAVGIDVPLTYGATKSKMKSLEIQKQSLQATAQFERRELEVQLLNAQQQ
                     YFQYLSQYRFYIDNALPSAQEIIKAAKLGYTSGEVNYIEFLLALENKTEVELKCMQTI
                     QQINQIALNINYLTSK"
     gene            complement(14160..14471)
                     /locus_tag="PLL59_00720"
     CDS             complement(14160..14471)
                     /locus_tag="PLL59_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22350.1"
                     /translation="MKLFTILFSMYVLALSFAPCADELECNEIYPTESSHSKHTHEKE
                     NCTPFCICACCGQHVVNKFNYFTPQNKIKTAVIEEYKVIVNQTFISQFNANIWQPPKI
                     S"
     gene            complement(14515..16497)
                     /gene="acs"
                     /locus_tag="PLL59_00725"
     CDS             complement(14515..16497)
                     /gene="acs"
                     /locus_tag="PLL59_00725"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HMY22351.1"
                     /translation="MQHKITSFDQYQKTYQYSVNEPEQFWNEIADSFVWKQKWNKTLE
                     WNFKTPDVKWFLNGKLNITENCLDRHLEKNGNKPAIIWESNDVNEANRTLTYNELHAE
                     VCRLSNVLQNNGVVKGDRICLYMPMIPELAIAVLACARIGAIHSVVFAGFSSKSIADR
                     INDASCKLLLTADGLYRGNKTINLKEICDEALASTPSIEKVIVYQRTHQKIDMQIGRD
                     VWWHSEMQKAATHFDAVEMDSEDPLFILYTSGSTGKPKGVVHTQAGYMVYTYYTFLNV
                     FQYEEDDIFWCTADIGWITGHSYIVYGPLLNGATTVMFEGIPTYPDAGRFWATIDKLK
                     VTKFYTAPTAIRSLMANGIEFVTPYKLDTLRVIGSVGEPINEEAWHWYHHHIGKEKCP
                     LVDTWWQTETAGILISPLAFITPTKPTYATLPLPGVQPVLVDAQGSILQGNDIEGFLC
                     MQFPWPGIIRTTYGDHERCRQAYFASFDNLYFTGDGCRRDADGYYRIIGRVDDVMNVS
                     GHRLGTAEVENAINEHPNVIESAVVAYPHDIKGQGIYAFVIVDKQSIDQGVVDTDAPF
                     EEGAGIDNSLQLLNRAAFENEIRNIVSKIISPIAKPDKIQIVSGLPKTRSGKIMRRIL
                     RKIAEGDISNLGDTTTLLDPNVVEEIKQGAGISTKN"
     gene            16597..18732
                     /locus_tag="PLL59_00730"
     CDS             16597..18732
                     /locus_tag="PLL59_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HMY22352.1"
                     /translation="MKYFGLILITLIAHTVYSKKEKSKKLNIPYPTTIKTNHTDEYFG
                     TIVEDPYQWLEDDTCKATTDWVSMQNKVTYSYLSNIPYREEIKKRYTELYDYAKLDNP
                     IIIGNKLIFEKKEGTQNQAILYIQEIANGKPEIFINPNELDEKGLTTYSISGVSTDDK
                     YIAISSHKSGSDWSEMYIYEVATKKRLDDKLEWLKSSAASWDKNGFYYSRYPKPEAGK
                     ELSSKNEYLSVYYHQLGTPQEQDILIYSDKNDPNSFNAAYVSEDGAYLYLYKFKGTYG
                     NELYFKKISEPGWNFTPLFTSYKNSYTLINNIQDNFLILTDDNASNKQLILVDPKNND
                     IKQWKKIIAEKKDALIEDIGISNGKIIVKYLQNAACKLMTYDLNGKNEKEIALPGIGN
                     IEIYYGKQNSTFFSYTYSSFNYPNSIFIHNLNNGYSSLFLKPDLKFNPEDYVSKQVWY
                     KSKDGTKVSMFITHKKDIILNGKNPTYLYGYGGFNSNETPRFSASRILLLENGGVFAL
                     PNLRGGGEYGQAWHEAGMLMKKQNVFDDFIYAAQYLIKQKYTSKEKLAIAGGSNGGLL
                     VGACMTQKPELFKVAFPAVGVMDMLKYQHFTVGHGWVVEYGSSEQSKEMFEYLYRYSP
                     YHNIKKDIKYPATMITTADHDDRVVPAHSFKFAARLQEYGNKSVPLLIRIGINAGHGK
                     GKPTSMIIEEVADQWSFFFWNVGVKSLIK"
     gene            18734..19609
                     /locus_tag="PLL59_00735"
     CDS             18734..19609
                     /locus_tag="PLL59_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HMY22353.1"
                     /translation="MTAKKKYIAFCNQHADIIPVFLRAIWLDAVAEKWNVILQEEDNE
                     IKAALPYCTKGNILTNRIYLPDVSFYQSIIFLQHYTAKQQQAISENLFRQLPHTLKSY
                     FKFLPEYSNIDLHALKYKSEVYPTYCIKKKSEISLSTNHARNVQKGVKNQYIISESKN
                     ISISYALLASTFIRQNLQIKLSFELYKKLQHITQKNGMGKTLDCIDQRNNVLASILIV
                     EDAKCVYYLYGGYDTSFKNSGAMTFLLHYIIQDTIQRNKIFNFCGSSKKSIANFFEGF
                     GAKKTSIPIWKKSIY"
     gene            complement(19606..20520)
                     /locus_tag="PLL59_00740"
     CDS             complement(19606..20520)
                     /locus_tag="PLL59_00740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY22354.1"
                     /translation="MEKIFSFIIISYNRAEDTIAAVKNVLQLHDVENWRKEIIVLNNG
                     STQDYSAFHFFLNTLNEVERKWIHYINSSVNLGVAGGRNHCIKIAQGEYLLFMDDDAE
                     IIQQNAISIILDKYKKYKQENLAIIGFLGKNPFTGKLETPLKNQDLIKGKQEVFYNLF
                     FGYGHVFPKKLIDDTGLYQEDFFYGMEEYDLSYATVKAGYSILFTQDIVVMHKQNPNG
                     REPSNTTYARMFANKIIVLYKHMPMLYVCTHFILWSAFFLVKSRFDIRLYFATLHSLR
                     QRIRVAPRHVMNENGMKYLRKVSARIWY"
     gene            complement(20522..23155)
                     /locus_tag="PLL59_00745"
     CDS             complement(20522..23155)
                     /locus_tag="PLL59_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22355.1"
                     /translation="MSKKQAPKPVAQKAKSSSAPLLQLSNKIMIPAVMLLFLVLTFLY
                     CKPLLEGMQLSTHDSNQYIALNKESADLKATTGHVTMWSSRMFSGMPAYLMGGLEFSK
                     VIENSPRTTLYKIASAIPDPAFEIMLMLLGAFIGFYVLTAKKSYAFLGAIAIGFCTYN
                     FISLDAGHITKVKAIAMFMPLFAATWLTYQRKYIWGIILFLIFSYEIIAARHVQIAYY
                     GFILIGVYALFEIISQSIRGEMKQAATSSALLAVAITITIMMNFDNYFINDFSKDTTR
                     GGDILNAAKMNPGEPSAPTSHEKGVGFDYATNWSLGFEEIGSLFVPNFAGGSSAAGLD
                     ENSAVYKTLSAKGVSPQQASQFVQRMPLYWGSEPFVQGPIYLGAIIVFLFVFACFAYK
                     GKLKGWIIGGVIFTLLISLGKNMEFFYRLLYDFLPGFNKFRAPTMILALTQVLMVIMG
                     VLGLKDFFDNAIYNKENRIKILKLSTGITAGVLIFFIALGSVFSFQSKAIQNGKTTDD
                     QFKEQLVQMTGDQNFANDIYSSLKNDRASAMRSDAMRSLLFVVLVAAVLFVFAFEKFN
                     TPSYIVLAISVLVLADFWTVNKRYLNDDDFEDKSAIEANAFPQTPADAAILAQNKDGA
                     RMADFTLDIFNSASPAYYHRTIGGYSPAKLRRYQDIIEHGLSYDFQQINKSGFAHANY
                     INMLNARYIKQAPEASGVFVNHYALGNAWMVNTLETVNSPEEEILKIRTIDPAKVALV
                     NKEFANYIQDVKPNADTIINDMRYVKQIYTKDPMKLEYTFKSPKDEFVVFSEVIYRPN
                     EDWISYIDGKPADHIRVNYILRGMKVKAGEHKITFEFKPKLYAMTNNVLLLGNVLFYV
                     VIAGLVFLYVRKRKSVSNPTA"
     gene            complement(23179..24066)
                     /locus_tag="PLL59_00750"
     CDS             complement(23179..24066)
                     /locus_tag="PLL59_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HMY22356.1"
                     /translation="MVKVALTGGLGNQMFQYAFARNIAVKNETTLCLQTSYLQSKLPF
                     KNLATQMRYELDIFNIHAQISHNFFQSKYAYPFAKIEHILKDLWNSNKLNEVSEGHFH
                     FQPALLHTKDNCYIKGNFQSHKYFIEIEDIIRNDFSFKNKLRNENLAWQHQIENSNAV
                     SIHFRRGDYITLKQNVNKFAQIPFSYYQNAIQHIAEKIENPVFFIFTDDVHWIKSNFT
                     TEFPYYIVENNNTATTSYLDMQLMSMCKHNIICNSTFSWWAAWLNANKNKIVIAPNRW
                     FTDDKINSNDIYPSEWIKL"
     gene            complement(24125..24802)
                     /locus_tag="PLL59_00755"
     CDS             complement(24125..24802)
                     /locus_tag="PLL59_00755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY22357.1"
                     /translation="MGAVSFSIPKKLVTSLVNDYKIKNFVETGTFRGDSSIWAADYFE
                     KVYTIEINEEIFTSTSNRPDRKPNIEFLQGNSKDRIPEIIEKINDKSLFWLDGHWCVG
                     AGGKEHECPLEDELLAISKIGDNAVVLIDDARCFLGKLPPPHKSEDWPTISEIFKLFF
                     QHFPNHNVTIIQDVIVAVPKQMQKTIDTYWMQNYADFYSDAATINKYSLYKIIKLKIL
                     HLLRKFK"
     gene            complement(24815..25522)
                     /locus_tag="PLL59_00760"
     CDS             complement(24815..25522)
                     /locus_tag="PLL59_00760"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMY22358.1"
                     /translation="MKRLIIKIFNFLGFDIYQKPGKFYKNKLKLLTSHQVNCILDVGA
                     NEGQYALKLRKYGYKGKIISFEPQLDTFKKLEANAQRFSNWTTHHYALGASDETTEIN
                     VSQNTVSSSFLEIKDIHVAAAQDAKYVRKESIAIKRLDSIFDTLELHNQNIFLKIDTQ
                     GFEMEVLKGATSLFDKIIGFQIEMSLVQLYENEILFDEIIAYLKQHNFGLYQIETGVV
                     DETTGRMLQIDGIFFKN"
     gene            25587..26309
                     /locus_tag="PLL59_00765"
     CDS             25587..26309
                     /locus_tag="PLL59_00765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY22359.1"
                     /translation="MSYRPKISIITPVFNNEMEIGRCIDSVFGQTYTNIEHIIIDGKS
                     TDNSLSIIKEKAAHFDIKYISEKDEGIYDAMNKGIALANGDYLYFLGSDDVFYANNVL
                     QEIVVKFENNDIIYGNVFFKHAQHIYAGEYNKNRIIDQSICHQAAFFSKRTFKIIGTY
                     DTQLQLAGDWDLFLRCFYHDDINIRYIENIICLYNDLGASGYASENRKTKYRVLYNRL
                     GINGILKYVSIQIKKKLSIPFY"
     gene            complement(26292..27218)
                     /locus_tag="PLL59_00770"
     CDS             complement(26292..27218)
                     /locus_tag="PLL59_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22360.1"
                     /translation="MKKINQLFALSFSYIVLLVLIPIRKKCTITCAGKTDGMGAQVQA
                     IYSAMLYANAVGVKYCHTPIHSVEHNYNNTENFNTQVEDFFSLSLGEQNADEFYTSST
                     TIYLDNFSWRTIVDTAKYLLGIGNSFLFVKSHFHNYANLHTHQFTDIKARLRNKYYHN
                     KQQTNHNNEILQVIFHVRRGDVSATANNERFTSNSEIYDKIIALREMLDKQQLSYSIS
                     IYSQGNKNDFDNLDTVAQLHLNGDVFSDFSDLVNADILVTSKSAYSYAAAILSNAIII
                     YEPFYHKKLPDWILYSTGIFNTKEFISLIKRN"
     gene            complement(27259..28704)
                     /locus_tag="PLL59_00775"
     CDS             complement(27259..28704)
                     /locus_tag="PLL59_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMY22361.1"
                     /translation="MKKIIQHIKQLQFYNTLRHGSVYMLSFVVVQAVMVLSIPVFTKL
                     LTPADFGIYEVYNNTVRFLAVIISVNLYTGFYRFYFEENISKNALMQFLLRTALLSFI
                     IGLAVLLLLQSQVLKITGLPSNLFWWIPIGIFSTIIFNFFTTYNNAQQYSTRAGIWQL
                     VSQIGRVVLAIIFVIYISRDYYGRIAGENILLLIASLLIIFSYFRNYIGLKEQLPDKK
                     EILKYSAGFIPIGLSSYIVSYVDLVLINNMQGSTASGVYSYAYKFAVIYSGFSQAFVT
                     ANRPNLFNLLNENKSEEVITQMRSMFKLITVLSVVFILFAGDAGRILSLKPEFEHALH
                     LLPVLVLAYVFSDMAEVYNFFLHYSKKVKLFYVSFITTAIINFTLNYYLIPIYGYEVA
                     AYTTLFSFMVLFATTYLVCKFYTQLSVPRWTVFLESIILIAAMALISFVLPEFIQQLY
                     LLIATKIILFLILVVYVFYDKIIQVKSLLKP"
     gene            complement(28723..29592)
                     /locus_tag="PLL59_00780"
     CDS             complement(28723..29592)
                     /locus_tag="PLL59_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HMY22362.1"
                     /translation="MIVVRLNGGLGNQLFQYATGRHVAHLNKTELYIDTSAFSDNTVD
                     SKWKYELPAFHINATLANEELLKHFKLNDFSTSGIMLTKLLSFGKYGKYKFNEYGFDS
                     NVLELRGNYYLKGFFQSEKFFKNIAESLKEELVIKENFLPQKTDLLQDISNKNSVSIH
                     IRRGDYIRNLSAMDTHGLCSKDYYTKAIEFIKRELGNDLHFYLFTDDEEWIHREMHWD
                     IDCTLLSGNSTIEDFYLMSQCKHNIIANSTFSWWAAWLNKNPNKKIVIPKHWRTNVKT
                     EDIYLNPPKWFIK"
     gene            29639..31405
                     /locus_tag="PLL59_00785"
     CDS             29639..31405
                     /locus_tag="PLL59_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HMY22363.1"
                     /translation="MLFIGTGKGDITAFFKGIGMLGYGLPQHIMLDIETPMYARAFVF
                     EQTDKSQKVCFVNCELGFITPSLKQGVLNLLTKEHPALQYNTSNLLLSAQHTHCGPSG
                     FSHHVIYNLSTPGFHQGVYDKIVNGIVQSIIEAENNKQKASIQIGKSAFSTDIPVSFN
                     RVIDAYNANPEVTKLTQENRHLAVDREMTLLHFISEHGQALGSVNWFAVHTSNLPNNY
                     YKLCSDNKGFAASYLEDSMKKENKNYVAAFAQGACGDVSARVKYNNNLPATRGKWEGY
                     FEDDLKSAKFNGNLQFEKAKEITHNALETIKQDSIDYISMYVDFSNIQIDSTFTNGKQ
                     DCVTSPSCMGVAFLEGSIMDGPGLPQFLGKIARVVAKRTRKKEIKDAQKIGGEYAQFI
                     ARKNKAQSVKDIGVASGERRFLGNFDADKLPLPGFVDETILNIKKMAKQGGFSSNNPW
                     APQVLTLQILKIGNIGICGFPFEITTVASWRLKKTLADALQSKGIDFVILAPYSNEYN
                     GYITTNEEYQLQLYEAGHNVFGQWSLNALQQKFSELATEFCKNQEDRNTDKHVHPELF
                     TSDELKKQIYFPNKTVQKLNKK"
     gene            31451..32146
                     /locus_tag="PLL59_00790"
     CDS             31451..32146
                     /locus_tag="PLL59_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HMY22364.1"
                     /translation="MSKIFQNATEALYDVGDNMTIILGGFGLCGIPENSIAALVKKGV
                     SGLTCISNNAGVDDFGLGLLLKNHQIKKMIASYVGENAEFERQLLSGELEVELIPQGT
                     LAERCRATAAGIPAFFTPAGYGTEVAEGKEVREFDGKMYIMEKRFHADFSIIKAWKGD
                     TNGNLIFKAATRNFNLPMAGAGKITIAEVEELVPAGELDPDQIHTPGIFIKRIFQGAH
                     FEKRIEKITNRKK"
     gene            32157..32810
                     /locus_tag="PLL59_00795"
     CDS             32157..32810
                     /locus_tag="PLL59_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HMY22365.1"
                     /translation="MLDKNGIAKRIARELQDGFYVNLGIGIPTLVANYVPENIHVTFQ
                     SENGMLGMGPFPFEGEEDADVINAGKQTVTMMPGGVYFDSATSFAMIRGEHVDLTVLG
                     AMEVSETGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKAGESKLLPKCTLP
                     LTGIACVKKIVTELAFLEVTADGFKLIERAPGVSVEYIQKATLGKLIIEGEIPEMVI"
     gene            complement(32816..34105)
                     /locus_tag="PLL59_00800"
     CDS             complement(32816..34105)
                     /locus_tag="PLL59_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22366.1"
                     /translation="MQQRNYKRVEKNADGSFAYKTIDIDSYDIFEEPLEIIVTDINFL
                     PQMGNILDTLGYVPDTGYFFHQCKDIISVPEKHINNKGQGKFAFQAYFHNFPNSVLSI
                     NKDALVVIVLHPQKLDANGKREFNVVGYIHVNLMDVYLEGQRYDAYYYNMLRVSERVE
                     ENRKVYRRKKIFTLMFSIMHSIVGVEENIAFTYGAMGKENQAINEALELNTKLYEKHY
                     EKWAFTNNTKVNKFFGSKDAFKKCIDISDDVEKLKQFYHKLKAQKEHYTFYHLKSEED
                     FIDTLQRIFAYSKSSRVYMVPDSNGNMDAVCFAINWGEYFAFQLENPQGLFKAVANME
                     LTDNILYPIMITGSPENVDVLLKGIASHYNKHYKCKVTNLNSYAGDPYAKTKKSALND
                     EYLFLIICNDVEKLEAMKNRSKGTDGNVKLFIDVPLL"
     gene            complement(34275..35387)
                     /gene="dprA"
                     /locus_tag="PLL59_00805"
     CDS             complement(34275..35387)
                     /gene="dprA"
                     /locus_tag="PLL59_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HMY22367.1"
                     /translation="MSTELFYGIALSQLKGIGDAYAKILISYAGSAENIFKLSKTKLQ
                     KINGIGPKLASIFNDTYEAQKIAEREMAFVEKNNIEILFYYDKRYPKRLLHCADAPLY
                     IFSKGNVDFNKDKFVNIIGTRHATEYGKAITENLINVLAQHQVHIVSGLAYGIDICAH
                     KSALKYDIQNIAVLAHGLDIIYPNQHKNIAERLQQNGALVTTYLSGTIPDKGNFPNRN
                     RIVAGMTDATIVVESAEKGGALITAEIANSYNRDVFAYPGKITDTYSKGCLKLIKQLK
                     AQLVTNADDILQSMNWIANDDTTAKPSQQTQLFLELNEQEQAIVDMLKNADKLHIDVL
                     SASLPITLGKLSSILLQLEMQGVIQSLPGKQYKLIV"
     gene            complement(35452..37065)
                     /locus_tag="PLL59_00810"
     CDS             complement(35452..37065)
                     /locus_tag="PLL59_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22368.1"
                     /translation="MKKAISVIFVFFAVSALAQQLNFPLNYEMQNRLERYIQSSSHFI
                     TSAKPYNLAEVKAAIGDSALQELGYVTDSSKHGFKFGLLKGDMLSVATKKHKFYFAVN
                     PVLDMQVGYDPLAKKLQYTAGYGIKFDANLGKKVSLSFTYQGVTENYLQQVSNYAATY
                     GVLPGYRKATFKGNRINSQLFSGYFSYSPNKYFNLQVGNDKNFWGDGYRSVFLSDNAS
                     NSPYLKLTTNFWRIKYVYLLNVMRYGPVDGFNVERNPSNFRTKYGTFHLISVDVAKWM
                     QFTFFEGVTWFHNDSNRIRGIEFSYLIPIAFIRPVEFALGSPDNVVLGIGMKFKASPK
                     QIFYTQIMLDDMDVGAARKGKGFYRTKVAAQFGYKGYDLFKVKHLDFQTELNIVRPFV
                     YAHKAPEQSYTNFNQSLTHPMGANFLESITFLNFWKNHWTASAKLQYVVQGLDPAFEH
                     DGSNIFISDFMITPNLGTAYGNYFLQGVKTRLVNVEARGGYLINPKINLSVEAFVNYR
                     YLKNKFQSRQNLYFGISLRTNMFNRYTDF"
     gene            37181..37639
                     /locus_tag="PLL59_00815"
     CDS             37181..37639
                     /locus_tag="PLL59_00815"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HMY22369.1"
                     /translation="MISLKKNIKLINFYPPFLGAGISLKSVNEDFTKIEVQLKMRWYN
                     RNAVGTHFGGSLYSMTDPFYMFILMENLGKNYIVWDKAACIKFKKPGLKTVSAIFEIN
                     KDEISNIKQYVDTNGKGDFTFTTTIRNEENEVVAEVEKIVYVRSKTYSNN"
     gene            complement(37650..38078)
                     /locus_tag="PLL59_00820"
     CDS             complement(37650..38078)
                     /locus_tag="PLL59_00820"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMY22370.1"
                     /translation="MLDFDTFFMREALKLANIAFDEDEVPVGAVVVCGDKIIGKGYNQ
                     VEKLNDPTAHAELLAITAACNFLGSKYLDKCTLYVTLEPCMMCTGALRESQIKKVVFA
                     AADTSNRSAAPNLFEVKNGILANEAAALLTSFFKKKRKKI"
     gene            38193..39620
                     /locus_tag="PLL59_00825"
     CDS             38193..39620
                     /locus_tag="PLL59_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMY22371.1"
                     /translation="MKFSYKRIFLLFGIAIVYANGIAQETWNWEKCIDYALKNNLQLQ
                     QADVNIALGEATLKGNKMGYSPMINADVNYNLRIGNNFNFFNSSYERQLVHYQDYGVN
                     LSQPIIDLVTPATVKKSKLELSALKLDQETLKNNIQLQLLTAFLNIMNANEQKEQATQ
                     QHQTTKEQFDRMNTLIASGAAAENAILDIEAQLTNEALSISQIKNQLDIAYLNLKMLL
                     QLDPKQSIQVEIPRIPELLTLDAIADVNTIYEAALGLRPEIKSAQLKARSAQQQINVA
                     KGNYYPSLNFISNVNTFFTTQNKSSQTTTTGNFVPSGAIVDGTFQSVLIPETITTQSK
                     NPYKKQLNQNLSYAFGLTLNVPIFNKFQVQTAVKQSRLNYKMALVSEKQAGLDLFNSI
                     QQAHIKAQAAIENYTAADKNYSIAQKSYDYALERLHAGSISQLEVNTAKNNVAIALSK
                     LTQSKYEYLFNTKLLDFYQGKKIEL"
     gene            39651..41006
                     /locus_tag="PLL59_00830"
     CDS             39651..41006
                     /locus_tag="PLL59_00830"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMY22372.1"
                     /translation="MKNNKLLYILGIITFIALLAAYFIGKNRKANAAKEVEWQAVSTR
                     TIIQTVTASGKIYPEEEVKISSDVSGEIIELYVKEGDSVQRGQLLARIKPESYQAMVE
                     QSVAQLDNTKAQLSTAKARIAQASAVLLQAQAQFDAAQLAYTRAQELFGKKIMSKADL
                     ESAESSFKTSKSAVESAKADVDAAKHNSDAATFTIRATEATLKEAKTNLNKTSIYAPM
                     SGIVSLLNVKKGEKVVGTLQMTGTEMMRIANFDNMEVRVDVSEGEITKVKLNDTASIE
                     VDAYLDRKFIGIVTQVANTSKGAGSALVSADQSTNFLVKIRLLKSSYEDLLVENPKPF
                     LPGMSATVDIKTKTKTGVLAVPIQAVTTKDTVYDGSSHTREIIFLKQQNIAKAIDIKT
                     GIQDDAYIEVTNGLQGKETVIVGPYNVLVKELKNGDKVKLLDKTKKKEDKDKEEDEDK
                     K"
     gene            complement(41017..41739)
                     /gene="rnc"
                     /locus_tag="PLL59_00835"
     CDS             complement(41017..41739)
                     /gene="rnc"
                     /locus_tag="PLL59_00835"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HMY22373.1"
                     /translation="MISFFKKLFSKPATTSSSFIFIDIYQHLNKYPLQSEDNYIRALT
                     HSSFTKKMEEKNERLEFLGDAVINFCVAEVLYHQMHNKDEGTMTKARASIVNRKNLNK
                     VGVDIGIPKYLKHKLLPKQLEEAPDIIGNAFEALIGAYYIDYGMKEMEKLIATLLMKE
                     FDAHNFHKTITDSKSYLLEWSQAQKKHLQFEHKSSVNENGLFEVALLIEGEVRATAIG
                     KNKKEAEQLACKKAIELLQLGA"
     gene            complement(41799..43064)
                     /gene="fabF"
                     /locus_tag="PLL59_00840"
     CDS             complement(41799..43064)
                     /gene="fabF"
                     /locus_tag="PLL59_00840"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HMY22374.1"
                     /translation="MKLNRVVVTGIGAITPLGNTISQFQQRLFEGHSACAPISYFDTT
                     SFRTKIACEVKDFSPASHFDSKELKRLDTFAQYALVATEEAMQDAQLLNYESLNKRRV
                     GVLFTSGFGGVQTFQQELFQYYQNGKNPRFFSPFFVPRTLLDLVGGAISIKYGFKGMN
                     YSVVAACASSTIALIDALNYIRLGKADVLIVGGAEASITEVGIGAFSAMKALSERNDE
                     PHTASRPFDADRDGFVMGEGSACMVLESLEHAQKRGAKIYAELAGGAMNADAYHITAP
                     HPDGDSVIHVMQEAIEDAQLSTDAIDYINVHGTSTPLGDIAEIKAIQQLFGEHAYSLN
                     ISATKSMTGHLMGAAGIVEAVIAVLSLQTQRVPPTINHFTHDSAFDTRLNLTLNTSQA
                     RQIHAVLSNNFGFGGHNASIVFKKFHSSL"
     gene            complement(43132..43365)
                     /locus_tag="PLL59_00845"
     CDS             complement(43132..43365)
                     /locus_tag="PLL59_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HMY22375.1"
                     /translation="MSTIAERVKKIIIDKLNVEDSEVTPEASFENDLKADSLETVELI
                     MEFEKEFNIIIPDEQADKIKTVGEAIAYLEANA"
     gene            43548..43991
                     /locus_tag="PLL59_00850"
     CDS             43548..43991
                     /locus_tag="PLL59_00850"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HMY22376.1"
                     /translation="MARKNLIQASFNFDIWGINSSMEDYRLCLHINEFLKWQLKRVHD
                     VEIFVNDAKNIKYFSAYKYNNDIDLYTVELIQNKKLGNILIPELKNIDYLFLFNGEDD
                     YFDKDAFINSLLNSPGVQSIFPIDVNLLKSRHNLLTRHFNEPSNT"
     gene            43972..45402
                     /gene="pyk"
                     /locus_tag="PLL59_00855"
     CDS             43972..45402
                     /gene="pyk"
                     /locus_tag="PLL59_00855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMY22377.1"
                     /translation="MSQAIRKTKIVATIGPASYSYEKLVELYKSGVNVFRLNFSHGRH
                     DDHEQVIRHIVRLNEAYHAHVSILADLQGPKLRTGLMQNNAIELIEGNSINVTCEEVI
                     GTKELISVTYEHLSTDLKIGEFILIDDGKLELQVAEILDPKTVRCVVISGGILSSKKG
                     FNLPNTKISLPSLTEKDRIDLAFILKQPVNWIALSFVRTAKEINELKDIIQEHKSSLK
                     VVAKIEKPEAVANIDSIIKATDAVMVARGDLGVEVAMEEMPLIQKNIIEKSIQNAKPV
                     IIATQVMESMIDMSRPTRAEITDVANGVLDGADAIMLSGETSVGKNPAKVVQTIDKII
                     RRTEQEDEIYHKHLLPDKSSKTFLSDAICFNACTIASEVGAKAINGMTYSGYTAFMLA
                     CNRPKADIHIFTSNKNLLNTLGLVWGVDVFYYNKFVSTDESIDDIIDILKKEDLVDID
                     DIIVSTASMPIMGRGRTNMLKISKIK"
     gene            complement(45403..46134)
                     /gene="rlmB"
                     /locus_tag="PLL59_00860"
     CDS             complement(45403..46134)
                     /gene="rlmB"
                     /locus_tag="PLL59_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HMY22378.1"
                     /translation="MKQQLIAGRNPVLELLQSKQEVEKVLLSKSASGEVIKQIIQLCK
                     DNEVYFQFVPEVKINSLSKANHQGVLAFTALIKYQEVQNIIDMVFSNGEDPLLLILEN
                     VTDIRNLGALARTALGLGVHAIIFPKKESAAVNDVAVKISAGALLKIPLCRVDNLIAT
                     IKDIKNNGIHLIGLDGTASSFIFEVNTKMPLAIVAGSEDEGISNPVIRLLDKLVKLPM
                     NDALESYNVSVATAMALYEVKRQTI"
     gene            complement(46253..46666)
                     /gene="mscL"
                     /locus_tag="PLL59_00865"
     CDS             complement(46253..46666)
                     /gene="mscL"
                     /locus_tag="PLL59_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HMY22379.1"
                     /translation="MGMIKEFKEFAIKGNLIDMAVGVVMGGAFGKVVSTFIDGMVMPA
                     IGMISGGVDFTQQKIILKDAVPASADGATPAVAEVAVKYGDFISQCITFLVVALVVFV
                     VIKAINKMKKAEAAAPAAPPAQEVLLTEIRDLLKK"
     gene            complement(46711..49803)
                     /locus_tag="PLL59_00870"
     CDS             complement(46711..49803)
                     /locus_tag="PLL59_00870"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HMY22380.1"
                     /translation="MNKQYKLINAVVGFIVFAISLWQYTSTMESAGSLWDCGEFVSCV
                     YKLQVAHPPGAPLFMILGRIFTLFNPSNPVIMVNFLSALMSAACVLFLYFVIAMVAKK
                     LITKAGEEFSIADIIAIVGSASVGALACSFSDTMWFSAVEGEVYASSMFFISIVLWAV
                     MKWNEEADEPHGNRWLVFAAYMIGVSLGVHLLSLLVIPCAVLVYYFKKFKPTILGLII
                     ATFMGFVLLGTVQIGVVLSALKLAGSIEFFSVNILHLPFNIGFVVAWILLFAILIALI
                     YYANKIKHADLQLLSICFLVIFIGFMSYAMVPIRSVANPPINMNAPKDAFSLLSYLNR
                     EQYGSRPLVSGPLYDAQPTGYESKGKDYYPDLKTKKYEAKGEKIEYTYNPEDMMLFPR
                     MGPTNDGESAEALYQYWTGLQGKPTFADNLKYFFHYQLRYMYWRYFLWNFSGRQDDLQ
                     GTPGNERMNGDWLSGIPFIDNIHLGSQDGLPFQILNEKARNKFYMLPLILGLIGIYFM
                     YQRNKQYTMIFGLLFLVTGIALIVYMNQPPREPRERDYGTVGSFFTFCIWIGLAVAAI
                     YDYLKRKLPALPVAVAVTILCLFVPYIMGKAGWDDHNRHGRYMARDFASNYLESCPPN
                     AILFTQGDNDTYPLWYAQEVEGIRTDVRIVNLSLLGVDWYINFLHRAANKSGSIPFYK
                     DFTDAKYRGNTRDVIQFNDQSGFADPSQYYDIQNIMAFVLSDDEQFKAQTQRGEAVNY
                     LPTTKLKLKVDKAAIEKYQVVPAQFKDKVVDEITWDLGKTRIIKFDLALLAMVAAGDW
                     SRPICFSNTVDGSYYNGLDKYLVQEGQILRFVPCRFDENQRGYFAMNTQKSFDIVTKK
                     FKYGGLGEREMFVDENSGRIMNTLKSAHLSIADDLIRNNRKEDAVKVLEQARKEFQYV
                     NAPYYSPNNRFFNILSVQWIDLYYRAGAPDKAKELKDLFIKDLKDCLRFYNLPNNDFA
                     ALYANEKKSAEELVKRMDILALMYKDATFKKQLNEAFPILIPTAALDTTQQIPMQLFR
                     "
     gene            50078..51397
                     /locus_tag="PLL59_00875"
     CDS             50078..51397
                     /locus_tag="PLL59_00875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY22381.1"
                     /translation="MSKKSKNKDYPLATYFGYYNQFLDTKIGKALNLRKISERFVYEG
                     IKNGVKVASNTTRVFKNLTGGNAERLTPKTATDLFDLNYTDEQQIVQESVQQFAVKMR
                     AAAEKADDTASIPDELWQEFNDLQLAYMQVPENLGGMMTQKSTVTQMIIAENLAHGDF
                     GQALAFYTKHSVLNAIIQWGTASQQAALVPAFLGDNPETATIAINEARPLFSPFDLKT
                     KAKKEDKQYCINGRKNMVPLAESAGYILVAADTEQQGIRLFIVDSNAEGVTITADRSM
                     GLNVAALCAIEFDDVMVDESAILGDSDFNYVEFIAYSKLGWCAMAVGCCQAILDYVIP
                     YANDRHAFGEPISHRQAVAFMIADIKIELDGMRMLTQRATALAEQGVAFEREAYLAHV
                     FCSDKAMQIASNGVQLLGGHGFIRDFPVERWYRDIRAVAIGINGIHL"
     gene            51410..52609
                     /locus_tag="PLL59_00880"
     CDS             51410..52609
                     /locus_tag="PLL59_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY22382.1"
                     /translation="MNLEVPSKFKGIQNFAIMAAKTIFRPVSRKYDVEEHAYPVELDL
                     FAAGLKGLNEGGDAPVGGANTKKENVVAGEVRNGANMANCVNIQAMCYGDVGLLLSIP
                     GQGLGNAAISAVANTEQLKRFANTWEAMAITEPNCGSDSSAITTTARKEGDYYILNGE
                     KIFVTAGERCDSVVVWATLDKSIGKAAIKSFVVEKGTPGMDLARLEKKLGIRASDTAA
                     ITFTDCKVPAENLLGSPDIDTKKGFGGVMETFDNTRPFVASMANGVAEAAIERTKELL
                     DDTITLDYKTPINNVKAVEAELYRMEADYEAARLLTLRAAWMADNRKPNSLEASICKA
                     KAGRVTNQIALKCIELCSSLGYSYQELIEKWARDSKILDIFEGTQQIQQLIIARRLLN
                     KSSAELK"
     gene            52612..54438
                     /locus_tag="PLL59_00885"
     CDS             52612..54438
                     /locus_tag="PLL59_00885"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HMY22383.1"
                     /translation="MSQHSSRKNTVQFFELATKIPGLAKDLPKILTALYYNYTVNPTS
                     DITIGQLFQKSVLKYPERPCVIYHDQEWTYREFNLWINRLANHFIKIGIKKGDVVAVL
                     LENRPEILAISLALAKIGGITALLNTSQRNQTLLHSIQLAKPKLLLIGSELTAHYLEI
                     KEELNNNTCQTFLIEHLRVSTPKIKELSVFDTNSKKFAGTEPIIHYHINAKDAVMYIY
                     TSGTTGLPKASIISHLRWIKGYSAFGLTSFRLTPEDRLYVPLPFYHATAMIVCWSSVI
                     AGGASIILKNKFSVKDFWNDIDYYQATGFGYVGEMCVYLLNTEIHPKEKNNTLVKMIG
                     NGLRPEIWKKFKDRFSVEQVCEFYASSEGNIAFFNVFNIDETMGFSITNFAIVAYDQE
                     KEDTVKDKKGYLQKVNTNEVGLLLGEISDRYPFDGYTEKEKSESKVLRNVFKNGDAWF
                     NTGDLVRDMGYLHTQFVDRLGDTFRWKGENVSTIEVEGIINQYENIEECMVYGVEIPN
                     CNGRAGMVNIILKDQKQKFNFEAFYAFIRKELPPYAIPIFIRFSSAIETTTTFKHQKI
                     KLKQEGFDYTLIGNKVYAFIDKKYTLITKKILQDINNGVYRF"
     gene            54591..54661
                     /locus_tag="PLL59_00890"
     tRNA            54591..54661
                     /locus_tag="PLL59_00890"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54623..54625,aa:Gln,seq:ttg)
     gene            complement(54708..>55015)
                     /locus_tag="PLL59_00895"
     CDS             complement(54708..>55015)
                     /locus_tag="PLL59_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22384.1"
                     /translation="NYDAEDKKYYGKYWSAYDVPRHLYHFNTATIVVLMERLGFELLT
                     TYPLTFDAYYIALHSEWYLKSNRFMAIAKALKNGFFANLAASKSGNYSSLMYVFKKK"
BASE COUNT        18649 a         9461 c         9051 g        17854 t
ORIGIN      
        1 aacttcgtaa agcaatggga aaaaaagaca gagcaaaatt agactctcta aaacctatgt
       61 ttattgatgg ttgtaaggcc aaaaatttag atgaaaaaat ttgtgagaaa atctgggctg
      121 attgggaaaa atttgcttcg tatgctttca ataaatcgca ttcaacatgt tatgcttttg
      181 tagcattcca aactgcatat ctaaaagcgc attatccagc agaatatatg gctgcagtat
      241 tgtcgcataa tatgagtgat attaaacaaa ttaatttctt ccttagtgaa tgccgaagaa
      301 tgcaagtgat tacgcttggt cctgacatca acgaaagcga tgtaaaattc tcggtaaata
      361 aagctggcga aattcgtttt gcactttctg caattaaagg tgttggagaa gctgccgtaa
      421 cagacattct gttagaacgt gataaaaatg gagcattcaa aaatattttt gacttaacga
      481 aacgtgttaa ttctagaaca gtaaacaaaa aaaatattga atccttagcg caagcaggtg
      541 catttgatag ttttccaaat gtacatcgag cacaatattt ttataaatat aaagacgagt
      601 cttcttttat cgaattagca attagatatg gcaacaatgt aaaagatcaa aatgcatcag
      661 ctgcattttc tttatttggt ggcacttcag atgctgaaat acctgaacca gaaattccac
      721 aaacgcatcc ttggcaatct tttgaaaaac tgcaaaaaga aaaagaggtt actggtattt
      781 acatttctgg tcatccactc gatgattttg aattagaaat aaaaacattt tgtaacgcta
      841 cttttgaaat tattgaaaat tttcgagaga gtgaagtaac tatcgcatgt atggtttcga
      901 gtgtaaataa gcgcattgca aaaaatggaa aaccgtgggc aaatgtgctg tttgaagact
      961 tagatagcac aatggaaatc gcatttttca atgaaaacta tacaaagtat ttgggctttt
     1021 tagaacaagg aaatcgtttg tttattaaag gcatatataa acaaagtttc cgcgatcaaa
     1081 acaagtatga attgcgaatt actcaaattg atttcttaca agatgtatta gagaggtatg
     1141 gaaaaggact tcgaatactc atgcactata aagatatgaa tgaagcaaaa gttgataaaa
     1201 tatataacat cgtatctaaa tataaaggca aaaaaccatt tagcttttat ttatatgatg
     1261 acaacgaaca acctttaact ttccaatcta cgaaagttgg tgtaaaactc gagaatgaat
     1321 taataaacca actaaaatac attcccaata ttgtagtaaa attattgagt taatgcctaa
     1381 aaacaagttg aattagctta ataattctac taataaacta ttataattac gttttggcac
     1441 ggcgattgta atacgatttt ctataaaata gatcgtatga aaaaaatatt tacactcgca
     1501 ttttccatta ttatcagcag cacactgttt gctagcggtg aattttttat aaaattaaat
     1561 tcaaatggaa attatacagt tagcttaaac aatcagataa tcacaaacac aggtagtgtt
     1621 tttcgttttt ttgatttata cagtggcaac tacaatttaa aagtatatga aaacgggttt
     1681 aatggcagaa caatttttga gcaaacggtg aacgtgcttg atggctatcg aattgttgca
     1741 gaattagaca gatatacagg tttaaaaata attgaaaaaa ttccatttac acaaagcagt
     1801 tggtacgtcg atcatttaca aaactataac aacaattggt atccaaataa tccaaaacca
     1861 tctcgaccaa aatgtggtaa taattggaac cacaattgga ataatcaaca aggctggaat
     1921 aacaactgga ataatggttg gggcaatggc tctaacaata attatccgaa ttatccggga
     1981 aattatggct atggcaatgt attagaagat aatgaattac agtcgctcct cagtgttata
     2041 aagaatagtg cttttgatga taaaaaaata gaaatcatca agtctgcatt aaaaaataga
     2101 atgctaaaaa ctgatcatgt ttctcagtta atgcaacaat tttcatttga acaaaacaag
     2161 cttactattg ccaaatattg ctacgacaaa acaatggata aaaataatta ttacacatta
     2221 tacgacgatt ttgcatttag caactcgagt tcacaattag caaaatatat cgaatcaaaa
     2281 tagggaacaa tgacattaat taaacagata aaataacacg gtgactttag cgttatatac
     2341 ctattctttt gagtaggtat tttttattac atttaataaa aatccggttt gaaaatatag
     2401 cttgtaccaa cattgcaata aattaaacct acgcaacatt agatagtcta acaaaaaatc
     2461 aatcatgcaa aaattcgcta ctataatttg tttatttatt ttggtgaata cctttgcaca
     2521 gccactatat tttccgccaa aaaacaacag cacttgggat actatttctc ctcgtacttt
     2581 aggctggtgt aatgatgaca tagactcgtt atatacctat ttaggaaatg caaattctga
     2641 tgcatttatt tacttaaaga atggaaaaat tgtgttagaa aaatattttg gcagctttac
     2701 ggcagactca atacattatt gggcaagtgc aggaaagagc ttaaccgcaa tgatgattgg
     2761 tattgcgcag caaaaaggaa aattaaatat taatgatact gtatcgaaat atttaggcac
     2821 atcatggacg agtgaaagtc ttgaaaaaga aaataaaatc actataaagc atctactcac
     2881 gatgacaagc ggattagatg acaatccaac acaaccctgt gacaacttaa gtcaaacagc
     2941 aacatgcttg caatacaaag cagatgcagg tactcgttgg gcatatcaca gcggagcata
     3001 tcgcaagctt gaagatgtga ttacagctgc gaatggcact aattacaata cggcaagcta
     3061 tgactggttg gaatctaaaa cgggcatgaa tggtttttgg tttaatagtg tgtattatag
     3121 taatgcacgc gatatggcaa ggtatggatt gctaacctta aaccgcggaa tttggcaagc
     3181 tgatacagtt ttaaatgaca caagttattt tcgtgcaatg atcaatacat cgcagaataa
     3241 aaatttgtcc tatggttatt tatggtggtt gaatggaaaa aactctgtaa tgctgccagg
     3301 tacgcaaaca gtatttccta caaaattaat tccaaacgca cctgatgatt tatattgtgc
     3361 tttaggaaaa aacgatcaaa aaatttatgt cattccaagc accaaaacgg taatcgtgcg
     3421 tttcggcgat tcaaattttg gaaatgatgc ttcttcacca tttgacacta tgttgtggaa
     3481 ctacatcaat cgcttagaca attcatgctt gactacagct gttattcagc aaaataaatc
     3541 aaacactgtt ataaaactat ttccaaatcc tgctaataat ttcatatcat tcagtggctt
     3601 tgtaagcaac aacaacatac attatgcaat agcgaatatg cttggtgaaa ttgtaaaaga
     3661 agatgtcacg ctcaatcatt caattgacat tagtgatttg ccaagtggtc aatattatat
     3721 gctattaaat gatgatatgc aacaagcaag caaacaattt atcaagctgt agaattgaac
     3781 aaaaaatcta aattctgtca gcgtaatgac aatttttagc taaattttgt caatacgatg
     3841 acagatttac agaatgtaat ctaagtaatt actcgaattg actacagaaa atggtgcttt
     3901 gggatatgct tgagcaaatg caatcggcac tttaggtttc tttttgtctg aatacttaaa
     3961 ctcaaatgca cttatttttt tattatcttc ttctattaaa tctatttctt gttgatcata
     4021 ggttctccaa aaataaaaat tgacaaattt ctggctataa ctagtgtgtt ttaatctttc
     4081 tgaaaatata taattttccc acaatgcgcc tttttcatca tcacgtctaa gtgctagtgg
     4141 gttaaaatca ccaattacag cattacgaat gccattatct agaaaatacc aacgttgact
     4201 ttttgaaatt tctttacgta gattattact atatgcacct actttataga gtacaaaaac
     4261 tttagtcaat aaatgcaaat acttttcaac tgtatttttg ctaactccga gtgcatttcc
     4321 gagctcattg tacgatactt cttttcctac ttgataagca atcaatttta aaagattatg
     4381 cagtatgctt gcatttctga tgccatctag cactaaaata tctttcaata aataagaatg
     4441 cactaggcta tttaaataat ttcttttttc atttaaacta tctaaatgcc ataattcagg
     4501 ataagcgcca taaatcaaac gctcttctaa attctgttta gtttggatag cattttcttg
     4561 ttgtgcaatt tcgctttgat gtaaaggata aagatttagt gtataacttc tacctgtcaa
     4621 tggttcgcca gaaagattta ataaatcaaa agcagaagaa ccgcttacaa tcactttcaa
     4681 tccttttatg ttatccacca ttagctttag tttcttgcca atgtccggta tattttgtgc
     4741 ctcgtcaata actaataaat cgttgccttg caataagcgt gtatagtttt ttactgattt
     4801 aactgctaat aattctagcg tagtttcatc ttcaccgttt aaaaacaaat atggcttttt
     4861 gtattgcgat aagaattgtt caatcaatat cgatttacca acacgtctag cacctaacaa
     4921 gagtgttact ttttgtggtt taatatattt ttcaagtgct tcttgctgta ttctattgaa
     4981 ttccatactc aaatttacgt aaattctgtc aatgtaatga caaaattaac gtaaattctg
     5041 tcaacgtaat gacaattttt agctaaattc tgtcaatatg atgacaaatt ttaatttaca
     5101 ggtcgtaatt tgatggtttt aatgcgtact ggtaaaagcg gtctatcatt cttatctgtt
     5161 ttaacagatg ctattttatc taataccttt agtcctttta ctacttcgcc gtagatagtg
     5221 tagttcatat ctaaatgtgg aatgccgcca attgttcgat acacgatttt attggattct
     5281 ggtgtgtcat tgccagttcg ctgctttgct tttatgaaag tagagtcatc tgcaatttta
     5341 ccttgtacta tataaaactg acaagctgat gatgctttct ctggattatt atctcttgcc
     5401 tgtgccaaca tgccttttgt atgaaaatga cttgtcctaa attccgctgg aattgtatag
     5461 cctaaatcac cttcaccaag gaatacagtg tctgctgcat gttttgagtt aggatcgcca
     5521 ccttgaatga caaagccttt aataacacga tgaaacagaa gactatcata aaatttatct
     5581 ttcacaagct taatgaaatt atcgcgatgc tttggcgtat catcaaataa aacaacagtc
     5641 attgtgccat aattggtaat aatttttgct ttatatgttt gtgcttttac tgatgaaaca
     5701 gaataaaaca tcaacaaaaa ataaagtagt aattgtttca tagtattaca ttaagacaat
     5761 aaatttgtat agtattaaat tacacaaaaa atggaaactt ttgaaataaa agatgatatc
     5821 attttactta atcagttatt aaaggcaatc gcttggtgcg aaaatggagc acatgcaaat
     5881 gcagttatag aagaaggttt agtaaaagta aatggaaaaa tagaaactcg aaaacgcaat
     5941 caactgacaa gaggcacaat agtcagtttt gatggaaata ctgtaaaaat tgtttaaaca
     6001 aattcttttg aattattact tatcgaattt catttgttga atgcgtacag catttacaat
     6061 ggccaataag gcaacgccaa catcagcaaa tacagcttcc cacattgtag ctacgccacc
     6121 agcacctaat aataatacaa taagttttac agcaaatgct aaactgatgt tttgccaaac
     6181 tatttttttt gtttgttttc caatttgaat tgctgttgca atttttgaag gttcatcatt
     6241 ttgtattact acatcagccg tttcgatagt cgcatcgctt ccaagcccac ccatagcaat
     6301 tcctacatca ctcaaagcaa caactggcgc atcattaact ccatctccaa caaatgcaac
     6361 agattcattt ttacttttaa tttcttttac tttgtttact ttatcttctg gcaataaatc
     6421 accgaaggca tttttaattt gtaagctact agccacacgt tgcacaacag tagatttgtc
     6481 accactaagc attgttatct gtacttgcat ttgatggagt cgcttaatgg cttgttgagc
     6541 atcatcttta atctcatctg ctatcgtgat aaaaccaaca aatagattgt caagtgcaat
     6601 ggcaatcaca gtatctgtta tcgcattagg atcgactgta tgagcaatat taaacttgtt
     6661 taataattta aaattaccaa ccaacaacat tttattatta tgtaatgctt ttaagccatg
     6721 gccacttatt tcttcaactt gttgcaactg aatagtagaa tttatttctc caacaaattg
     6781 atgaatggca cttgccactg gatgtgtgct atggctttct attgcgttaa cgagatctaa
     6841 tatttcttgt ttattaaatg ctggagaaat ttgtacctct tgcactttaa aaactccttt
     6901 tgtcaaagtg cctgttttat ccatgactac atgttgaata cttgctaatt tatctaaaaa
     6961 attactgcct ttaaataata taccattgcg agaagcagca cctataccac cgaaataacc
     7021 aagcggaata ctaattacca atgcacatgg acaggatata actaagaata ttaatgagcg
     7081 gtacaaccaa tctttgaatt gataattgtt aataaaaatt gcaggtaaaa aacaaatcat
     7141 gactgctaag agcacaacaa ttggagtata aattcttgca aattttcgaa taaataattc
     7201 tgttggtgct ttgtgtgttg tagcatcttg aatcatgtta atgattttcg ataatttgct
     7261 atcagtatat gatgtagtta tttttacttg ggcaattgta tgtaggttaa tcatgccagc
     7321 taaaactaca tcgcctttta ctttggtatc aggcttgctt tcgccagtta atgccgctgt
     7381 attaaacgag gcattatctg ataataattc tccatctaag gctaatttct cacctggttt
     7441 tagttgtaca atatcaccta tattaacatc ttcagcttta agcgactgga tttgattatt
     7501 gacgagaata tttgcagtgt ctggcctttg gtcgatgagt gtttttatat ttaatttagc
     7561 acgtttaaca gcaatcgttt gaaaaatttc gcctatggaa taaaacaaca ttactgtaac
     7621 accttcttca aattcaccaa tagcgaaagc gccaatagta gcaatgccca tcagtaaaaa
     7681 ctctgaaaaa atatcacctt ttacaatgct ttcaaatgct tcttttagaa ctgaaaatcc
     7741 aactaacaaa taggctataa aaaaccaagc aatacgtata tatccaacaa accatgatgc
     7801 ttgtagaaaa tggtcaaagc agatagcaca caacaacaac aacagcgata aaacagtggg
     7861 caagaataat cgtatattac tcgtgtgacc atcatggtca tggctatgcc catgttcgtc
     7921 gtggctgtga tgcaaaagtg attttgcacc agcattatca tatatttttt caacctgatt
     7981 acaacatagc tgtttgcctt gattgtcgta tttatgttgg tgttccataa taataaaatt
     8041 tactttttac aacatgcatc taaagaatta tatgctgaaa tacttgcttt tattgcatct
     8101 gcatcatatc caagtgctgc aatatgctct ctaattgaat taattttaat aacagatggt
     8161 tcataggata cttttataag ctttaaagta gtattgatat ttacttgtct tattccttta
     8221 atctgtttta atttatttag taataatatg ccacaagtag gacattccaa acaatgatcg
     8281 caataaatat ttgttttgat agaggctgat gtgaccgtgc tttgtgctat cgaatactga
     8341 acattaagaa gcaatgagat ggtgataagt aatgttagaa aatattgttt cattgtagat
     8401 tattaagttt gattaatgag aatgagcttc gccttggttt gtcagtttag catttacaaa
     8461 gaatgctcct ttagtaataa tctgagcatt tgatggtaaa ttagaaattg gtgtaatagc
     8521 agtatatcca acatttgaaa caccttttac tacttctatt cgttcaaaat ttgtcgtatt
     8581 tttatctgaa gattttttaa tttcactcgc tgcatggcta tgttcatctt catcgtgatg
     8641 ttcatctggt tttttggaag taatcataaa aatataactt tttccatttg catcaactat
     8701 ggcatcattc gggaccgcat ttgttgtaac attatttaaa cttacaatgg ctgtaatatt
     8761 cataccatca atcaatcctg ttttgttgcc ttgcacagtg caatgaatgg gaattgtttt
     8821 gctatcattt tcaaaagaag aaccaatact ataaatcttg gcatcatatt catgaatagg
     8881 attattcgtt aatgtaaaat gaattacttg accgactttt aatattggta aatctttttc
     8941 aaaaatattc aaatctaaat gtagttgtgt attatctaca atttctacaa caggagacga
     9001 aacatctaca taactaccaa ttttagaaaa cacattgcta atagtgcctg aaattgggct
     9061 tttcaccacc aatgcagtgc gcaaattagt gttgtttaca gttgctggat ttattcccat
     9121 taattgtatc tgttcttgta gtgaagcttt gcgtgtattt aaaattttca attccgcatt
     9181 ggcactttga tagtttttta gtgctcctgc attgccatca tttaataatt tttggcgact
     9241 taattcttgt tcagcatact gtatcttgct actgacagtt agatattctt cttgcaattg
     9301 aatgaactga ggattgacaa ttgtggcaat aacttgaccg ctctttacga aactaccaac
     9361 ttgcacatgg attgatttta ttacgccacc atacaaagag gttgcattcg ctttgttatt
     9421 attaggtaca cgtaatgcac catttgcttt tagcgttgct gttaattgtt tttgttctac
     9481 tttgccaaat ataataccga cagaatttat ttgctcatct gtcaatgcag taatagttgg
     9541 ttccgtttca atcgagtttg catttacatt attgttttca ccttgtgatg atgcagaatt
     9601 tgcatgtgta tgaccatcat cttcgtgatg tgtattacac gatatgagcg caaagaatag
     9661 agcaaaaata agacaatgta tattttttga ttttatgata aacttcatgt gatttattta
     9721 cttgttaaat aattgatgtt aagtgcgatt tggttaattt gttgtatggt ttgcatgcat
     9781 tttaactcca cttctgtttt attttctaat gctaaaagaa attctatata attcacttca
     9841 ccagaagtat aacctaattt cgctgctttt atgatttctt gtgcagacgg taatgcgtta
     9901 tcaatataga atctatattg tgataaatat tgaaagtatt gttgttgcgc attgagtaat
     9961 tgtacttcta attctcttcg ttcaaattgt gctgttgctt gcaatgattg tttttgaatt
    10021 tctaatgatt tcatttttga tttcgtagcg ccatacgtca aaggaacatc tataccaaca
    10081 gcaccataat gaaagcgttt ggctgcatta aaaaacacat cttgattgtt tatattctga
    10141 aaaccaatca gagattgatt agcgtaactt agtgtaaaat caggcaatcc attagctttt
    10201 tcaatttttc tattttgttc tacaacgatt gcttcttgtt gaagtttttg tattgtagga
    10261 tgttgttcca tttgtactgt atttgctaat gcagacacaa taattggcgt gtacattgcg
    10321 tcgcggtgaa tggcaaatga atcattatta tccatatata atttgagcgt tttatagcaa
    10381 atagcaatct caaattcgtt ttgcgacaat tgcaacatga tttcatttcg tttagcttcc
    10441 gcagtggttt tttcaagctg tttagtttca ccacttttat atcttaatga agaagtagca
    10501 acaaaatctt gatatagact atctaaatat tgtagttttt gattgtaatt ttctaaatat
    10561 aataattgat aaaaatagga ttgtactttt agcacaattt cattttttgc aatctgttta
    10621 tctaattctt ttgaagatac tgctgctttt atggcatttc ttttggcagc aaataatgtg
    10681 ggaaatggaa tagtttgagt gatactaaat gcattgtcaa atttaatgct gctatattga
    10741 ccaaactgca cattcacatt caattttggc atttcattag cacttcttat caagctattt
    10801 gctgactgaa tattcaaatc ttcggctttc aatttgtaac tattttgaat tgccattgct
    10861 gtaatttcat ctactgtcat tactttggtt tgtgcatgtg atatgtgtgc actcaatagc
    10921 atcaacacaa gcgaacttac tactgttttc atatttgaat ttattttaga aaattttcca
    10981 tttacaatta tatataacaa tggtagaaca aataaagtta agaaagttgc tgatatcaaa
    11041 ccaccaataa caacggtggc taaaggtttt tgcacctcag cgcctgaact attgcttaat
    11101 gccattggca aaaaacctaa agaggctact gttgctgtca ttaacacagg acgaagtcgg
    11161 atttttgtac cttctaatat gcgatgtatc acatcattat cacctgattt ttccaattga
    11221 ttaaaggtgc ttatcaatac aattccatta agtacagcaa cgccaaataa tgctataaag
    11281 ccaataccag cagaaatgct aaatggcata tctcgaatga gtaacgcaaa tacaccacca
    11341 attgcactca ttggaatagc agtaaaaatt agcaaggctt gttttattga attgaaggta
    11401 aaaaacaaca acacaaaaac taagaacaaa gaagcaggaa cagcgattaa caaacggttc
    11461 gtggcttgtt gtaaattttc aaaagtgcca ccaaatgtaa gataatatcc tgttggcaaa
    11521 tggaatttat tatataattt ctcttgaatt tctttaacca cactttgcac atctcttcca
    11581 tcgacattaa accctacaac tactcttcgt tttccatctt cgcgactaat ctgtgctgca
    11641 cctaatttgt aagaaatatt tgctacttgt gaaagtggaa tttgatttcc ttgtgatgtt
    11701 ggaataaaca agctacttac atcttctata gaattgcgaa atgtcgaatc taagcgcact
    11761 actaaatcaa atttacgttc attctcgtaa acgacacctg ttgattttcc cgcaaatgct
    11821 gtacttacaa tatcgtttac ttcttctaca gacaatccat aattagccaa tcgcattctg
    11881 tcatattcga tattaatttg tggtaatcct tccacacgct caaccattgg tgcagtagtg
    11941 ccttcaacgg tctgtatgac acttccaacc ttatttgcat aagccaaaag tgtatccata
    12001 ttttcgccaa atacctttac agcaacatct tgtctgatgc ctgtcataag ttcattaaat
    12061 cgcatttgta ttggttgact tttttcaaaa aacactcctg gaatagcatc taatttttca
    12121 tatatctcat cagcgagttc atcatatgac ttctcctgtt tccattcact ttgtggtttc
    12181 agaataataa tcatgtctgt aacttctgga ggcattggat ctgttggcac ttctgctgta
    12241 cccgtttttc caattaccat ttttacttca tcaaattgtt taataatacg agaagcttgc
    12301 atagacgttt ctaagctttg actaagcgat gttccttgtg gcaacataca atgaaaggca
    12361 aaatctcctt cttgcaatgt tggaataaat tcaccaccca ttctgctaaa taaaaatagc
    12421 gagataaaaa atagtgtcgt tgtacatcca attaagagat acttaaatcg aattgctttt
    12481 tgtaataatg gaaggtaaag tttatgaaaa aatgccatca tcttgtcgga aaatgtatgt
    12541 tgtggcatcg cttgttttgg caagaataat gtacacatca ttggtatgta tgtaagcgat
    12601 aaaatcaaag cacctaaaat ggcgaaaccc actgtaattg ccattggttt aaacattttt
    12661 ccttcaattc ctaccaagga aagaattgga atatacacca tcataataat gatttcacca
    12721 aatgccgcac tgcttcttat tttagatgct gatacataaa cttcttcatc catttctgca
    12781 gctgtaagtt ttcgcataga ctgtcgcaat cctaaatggt gtaatgttgc ttctactatt
    12841 atcactgcac catctacaat taatccaaaa tcgattgcgc ctaaactcat tagattagcg
    12901 ctaatgccaa atagtttcat taaacttagt gcaaataaca ttgatagagg aatagctgaa
    12961 gctacgatta aaccagcgcg aaaattgcct aaaaataata ccaatacgaa gatgacaatt
    13021 agtgcacctt ctattaagtt tttttcaact gtggcaatcg ttcttttgat taagtctgtt
    13081 ctgtctaaat aggcttcaat gataacatct gctggcaatg ctttttgtat cactatcatt
    13141 ttttctttta tgcgttgtac tacttcattg ctgttttcac cttttaacat catgacaacg
    13201 cctccgacag catctacttc gccattataa gtaagtgcgc catatcgtat tgcgtttccg
    13261 aattgtactt ttgccacatc tttaataaaa acaggtacac tattagcatt acttttaaca
    13321 acaatatttt ctacatcttc aagcgaagta actaagccaa ttcctcttat aaaataggca
    13381 ttgggtttct tatcgatgta tgcgccgcct gtgttttggt tatttttttc gagcgcatta
    13441 aatacatctg gaattgtgat gcccattgct tgcaagcgaa atggattaat agatacttcg
    13501 tattgtttaa gcttaccacc aaagctattc acctctgcaa cacctttggt gccgtttagt
    13561 tggcgcgcta caatccaatc ttgcattgta cgtaaatcca tggtagaata cttttgttca
    13621 cttccttttt ttggatgtaa aacgtactga tatatttcac ctaatccagt gctcacaggt
    13681 gctaattcag gtttgccaat gccatttgga attttttcct cagcctcttt taatctttca
    13741 ttgattaatt ggcgtgcgaa ataaatatcg acatcttcgt cgaaaaccac tgtaattact
    13801 gacaatccga accttgaaat acttcttgac tcaataatat ctggcaaatt agcgatactt
    13861 tgctcaatcg gaaatgtgac taactgttcc acttcttgac ctgccaaagt aggacatagt
    13921 gtaataatct gtacttgatt attggtgata tcaggtactg catcaattgg taatttgcta
    13981 gcactccaaa tgccccaaac tatcaatagc atggtcatta ctgcaacaac aaacttattt
    14041 ttaatactaa aataaatgat cttatctaac ataaataaaa tttaataatc gaaataaatg
    14101 tcattctttc aaaaggaatt gacaggagat attattgcgt gtagcaatat tttcgtcgat
    14161 taagaaatct taggtggttg ccagatattg gcattaaatt gtgagataaa agtttgattt
    14221 acgataacct tatattcttc gattacggcc gttttaattt tgttttgtgg cgtaaaataa
    14281 ttgaatttat tgactacatg ttgaccgcaa caagcacaaa tacaaaatgg tgtgcagttt
    14341 tccttctcat gtgtatgttt actatgtgaa ctctccgtag ggtatatttc attacattct
    14401 aactcatctg cgcaaggcgc aaatgaaagt gcgagaacat acatactgaa taggatagta
    14461 aataatttca cgatgtaaag ttaattaatt tcaataaatc atgtgtagta tttttcaatt
    14521 ctttgtagaa attccagcac cttgtttaat ttcttctacc acattcgggt ctaataaggt
    14581 ggtggtatca cctaaattac tgatatctcc ttctgctatt tttcgtagta ttcgtcgcat
    14641 aattttacca cttctcgttt taggcaatcc tgatacgatt tgaattttat ctggttttgc
    14701 aatgggcgag ataattttgc ttacaatatt tctaatctca ttttcaaaag ctgctctatt
    14761 taataattgc aagctattgt caataccagc accttcttca aacggtgcat ctgtatctac
    14821 gacaccttga tctatacttt gtttatcaac gatcacaaaa gcataaatac cttgtccttt
    14881 aatatcgtgt ggatatgcta ccacagcact ttctattaca ttaggatgtt catttatagc
    14941 gttttccact tctgctgttc ctaatcggtg gccgctgaca ttcatcacat catcgaccct
    15001 gccaataatt cgataatagc catcggcatc acgtcggcag ccatcacccg taaaatataa
    15061 attatcaaac gaagcaaaat aagcttgtcg gcaacgctca tgatcgccgt aagtagtgcg
    15121 tataatacca ggccaaggaa attgcataca caagaatcct tcaatatcat ttccttgaag
    15181 gatagaacct tgtgcatcta ccaatacagg ttgtacacct ggcaaaggaa gtgtcgcata
    15241 agttggtttt gttggcgtta taaatgccaa tggcgaaatt aaaataccag cagtttctgt
    15301 ttgccaccat gtatccacta atggacattt ttcttttcct atatggtgat gataccaatg
    15361 ccatgcttct tcatttatag gctcaccaac ggaaccaatt actctaagtg tatctaattt
    15421 ataaggcgta acaaattcaa ttccgtttgc cattagagaa cgaatcgccg ttggtgcggt
    15481 atagaatttt gttactttta gcttatcgat tgttgcccaa aatcttccag catcaggata
    15541 tgtcggaatg ccttcaaaca ttacggttgt agcaccattt agtaaaggtc cataaacaat
    15601 ataagaatga cctgtaatcc aaccgatatc tgcggtacac caaaaaatat catcttcttc
    15661 atattgaaat acatttaaaa atgtgtagta agtgtacacc atatatccag cttgtgtatg
    15721 aacgactcct ttgggttttc cagtactgcc tgatgtgtat aatataaaca atggatcttc
    15781 gctgtccatt tctacagcat caaaatgtgt tgctgccttt tgcatttcac tatgccacca
    15841 tacatctctg ccaatttgca tatcaatttt ttgatgtgtg cgttgataaa caattacttt
    15901 ttcgattgat ggtgttgatg ctaatgcttc atcacaaatt tcttttaagt taatagtttt
    15961 atttccgcga tataatccat ctgctgttag taataattta catgatgcat cattaattct
    16021 atccgcaatg gatttggagg aaaatcctgc gaaaacaacg gaatgaatag caccaatgcg
    16081 cgcacaagct aaaactgcga ttgccagctc aggaatcatt ggcatataca aacaaatgcg
    16141 atcacctttt acaacaccat tattctgaag tacgttcgac aatcgacaaa cttctgcatg
    16201 caattcatta taggtaagtg ttctgttggc ttcatttaca tcattacttt cccaaattat
    16261 cgctggttta tttccatttt tttcgagatg cctatctaag caattttctg taatattcaa
    16321 tttgccattc aaaaaccatt ttacatcagg tgttttaaaa ttccattcta aagttttatt
    16381 ccatttttgc ttccatacaa atgaatctgc aatttcattc caaaattgtt caggttcatt
    16441 tacgctatat tgatacgttt tttgatattg atcaaatgat gtgattttat gttgcatgtt
    16501 tatattattc gttgttcaat atgctgtaat gtactaattt ttaacctatt ttgacattaa
    16561 aaatcgttaa tttcgttcaa ttaaaaacga agaaagatga aatattttgg attgatatta
    16621 attaccttga ttgcgcacac tgtgtattca aaaaaagaga agagtaaaaa attaaatata
    16681 ccatatccga ctacaataaa aacaaatcac acagatgaat atttcggtac tattgtagaa
    16741 gatccttacc aatggttgga agacgatact tgcaaagcaa ctacagattg ggtaagtatg
    16801 caaaataagg ttacctattc ctacttgagt aacattccgt atcgtgagga aattaaaaaa
    16861 agatacactg aattatatga ttatgcaaag ctcgacaacc caattattat tggcaacaag
    16921 cttattttcg agaagaaaga aggcacgcaa aatcaagcca ttttgtatat acaagaaatt
    16981 gcaaacggca agcctgaaat ttttatcaat ccaaatgaat tggacgaaaa aggattaaca
    17041 acatatagta tttcaggtgt ttctacagac gataaatata ttgccatatc ttcgcataaa
    17101 tcaggtagtg attggtcgga aatgtatatc tatgaagttg cgacaaaaaa aagattagat
    17161 gacaaattag aatggttaaa aagtagtgct gcatcgtggg acaaaaacgg tttctattat
    17221 tcgcgatatc caaagcctga agctggcaaa gaattaagtt caaaaaatga gtatttatct
    17281 gtttattatc atcaattagg aacgccgcag gagcaggata ttttaatata tagcgataaa
    17341 aatgatccga attcattcaa tgctgcatat gtatcagaag atggagcata tctgtatttg
    17401 tataaattca aaggtactta tggcaatgag ttatacttta aaaaaataag cgagccaggt
    17461 tggaatttta caccattatt tacatcatat aaaaattcat acacattaat aaataacatt
    17521 caagataact ttttaattct tacagatgac aatgcctcaa acaagcaatt aatactagtt
    17581 gacccaaaga ataatgacat aaaacagtgg aagaaaatta ttgcagaaaa aaaagatgct
    17641 ttaatagaag atattggcat cagcaatgga aaaattattg tcaaatattt acaaaatgct
    17701 gcatgtaaac taatgacata tgacctaaac ggaaaaaatg aaaaagagat tgcattacct
    17761 ggtattggaa acatagaaat atattatggc aaacaaaatt caactttttt tagctacacc
    17821 tattcctctt ttaattatcc gaattccatt ttcatacaca acttaaataa tggttattct
    17881 agtttattct taaaacctga tttaaaattt aatccggagg attatgtttc caaacaagta
    17941 tggtacaaaa gcaaagatgg cactaaagtt tcgatgttta ttacacataa aaaagacatc
    18001 attttaaatg ggaaaaatcc gacatatctg tatggctatg gcggttttaa cagcaatgaa
    18061 acgccaagat tttcagcctc tcgaatttta ttattagaaa atggtggcgt gtttgccttg
    18121 ccaaacttac gtggtggtgg cgaatatggg caagcttggc atgaggccgg tatgttgatg
    18181 aaaaaacaaa atgtatttga tgactttatt tatgctgcac aatatttaat caaacaaaaa
    18241 tatacatcaa aagaaaagct ggctattgct ggtggctcaa atggcggttt gcttgttggc
    18301 gcatgcatga cacaaaaacc agaattattc aaagtagcat ttcctgccgt tggcgtgatg
    18361 gacatgctaa aataccaaca ttttaccgtt ggacatggat gggttgtcga atatggaagc
    18421 agtgaacaaa gcaaagagat gtttgaatat ctgtatcgct attcaccata tcacaacata
    18481 aaaaaagaca taaaatatcc tgcaacgatg ataacaactg ctgaccatga cgacagagta
    18541 gtgcctgcgc atagttttaa atttgctgct cgactacaag agtatggcaa caaatcagtg
    18601 cctttgttaa ttcgcatcgg tataaatgca ggacatggaa aaggtaaacc aacatcaatg
    18661 attattgaag aagttgctga ccaatggagt tttttctttt ggaatgtagg tgtaaaatct
    18721 ttaataaaat aaattgacag ccaagaaaaa atatattgca ttttgcaatc aacatgctga
    18781 tataattcct gtttttttac gtgcgatttg gttagatgct gtagcagaaa aatggaatgt
    18841 gatattacaa gaagaagaca atgaaattaa agctgcattg ccgtattgta caaaaggaaa
    18901 tattttaacc aatcgtatct atttaccaga tgtcagtttt tatcagtcta tcattttttt
    18961 acaacattac actgctaagc agcaacaagc tattagcgaa aatcttttcc gccaattgcc
    19021 tcacacatta aaatcttatt ttaaatttct gccagaatat tcaaatattg acttgcatgc
    19081 tttgaaatat aaatcggaag tgtaccctac ttattgcata aaaaagaagt cagaaatttc
    19141 attaagtaca aaccatgcac gcaatgtaca aaaaggtgtg aaaaatcaat acatcatttc
    19201 tgaaagtaaa aacatttcca tttcatatgc cttgttagca tcaacattta ttcgccagaa
    19261 cttacaaata aaattgtcat ttgaacttta taaaaaactc caacatatta cgcagaaaaa
    19321 tggaatggga aaaacacttg attgtattga tcaacgaaac aatgtgctag cttctattct
    19381 tattgttgaa gatgcaaaat gtgtttatta tctatatggt ggctatgata cttcttttaa
    19441 aaattcaggt gcaatgactt ttttgttgca ttatattatc caagacacta ttcagagaaa
    19501 taaaattttt aatttctgtg gtagctctaa aaaaagtatc gccaattttt tcgaaggatt
    19561 tggtgccaaa aaaacaagta ttcctatttg gaaaaaatcc atatactaat accaaattcg
    19621 cgcactaact ttacgcaaat acttcattcc attttcattc atcacatgtc gtggtgctac
    19681 acgaatacgc tgacgcaggc tgtgtaatgt tgcaaaatat aatcgaatat caaaacggga
    19741 tttaactaaa aaaaatgcgg accaaagtat aaaatgtgtg cacacatata acatcggcat
    19801 gtgtttatac aatacaataa ttttattggc aaacattctg gcatacgttg tattacttgg
    19861 ctctctgccg ttcggatttt gtttatgcat cactacaata tcttgtgtaa acaagataga
    19921 atagccagct ttcactgtgg cataactcaa atcgtattct tccataccat aaaagaaatc
    19981 ttcttgatac aagccagtgt cgtctattaa tttcttagga aaaacatgcc catatccaaa
    20041 aaataaatta taaaaaacct cttgtttacc tttaattaaa tcctgatttt ttaatggcgt
    20101 ttctaattta cccgtaaatg gattttttcc taaaaaacca ataatagcta aattttcttg
    20161 cttgtatttt ttatacttat ctaaaataat tgaaatggca ttttgttgaa taatttcagc
    20221 atcatcgtcc ataaataaca agtactcacc ttgtgcaatt ttaatacaat gatttcttcc
    20281 gcctgcaaca cctagattaa cagatgaatt gatataatgt atccattttc tttccacttc
    20341 atttagtgta tttaaaaaaa aatggaacgc tgaataatct tgtgttgaac cattattcaa
    20401 tacaattatt tcttttcgcc aattttcaac atcatggagt tgtaatacgt tttttactgc
    20461 agcaatggta tcctctgctc tattataact aatgataata aacgaaaaaa ttttttccat
    20521 tttaggctgt tggattactc acgctttttc tttttctcac gtacaaaaat actaaaccag
    20581 ctattactac ataaaataat acattgccta acaacaaaac attattcgtc atggcatata
    20641 attttggttt aaactcgaat gtaattttat gttcgcctgc tttgactttc atgcctcgta
    20701 aaatataatt gacgcgaata tggtcagcag gtttgccatc gatatatgaa atccaatctt
    20761 cattaggacg ataaattact tcagaaaaaa ctacaaactc atcttttggc gacttgaatg
    20821 tatattccaa tttcattgga tctttggtgt atatctgttt aacatagcgc atatcgttta
    20881 taatcgtatc tgcattaggt tttacatctt gtatatagtt tgcaaactct ttatttacca
    20941 atgctacttt tgctggatct atcgttctaa tttttaatat ttcttcttct ggagaattta
    21001 ctgtctcgag cgtgttcacc atccaagcat tgcctaatgc ataatgatta acaaatacac
    21061 cacttgcttc tggtgcttgt ttgatgtaac gtgcgtttag catgtttata taatttgcat
    21121 gtgcaaatcc agacttattt atttgctgaa agtcatagct taaaccatgc tcaattatat
    21181 cttgatagcg gcgtaatttt gctggactgt aaccaccaat tgttctgtga taataggcag
    21241 gacttgcact gttaaatata tctaaagtaa aatcagccat acgcgcgccg tctttatttt
    21301 gtgctaaaat agcagcatct gcaggcgttt gtggaaatgc attggcttct attgctgatt
    21361 tatcttcaaa atcatcatca ttcaaatagc gtttgttcac cgtccaaaaa tctgctaaaa
    21421 ctaaaacaga aattgccaac acgatatatg aaggtgtatt gaatttttcg aatgcaaaaa
    21481 caaataaaac agcagcaacc aaaacaacaa ataacagtga gcgcattgca tcgctacgca
    21541 ttgctgatgc tctatcattt tttaatgaac tgtaaatatc attagcaaaa ttttggtcgc
    21601 ccgtcatttg caccaattgt tctttgaatt ggtcgtctgt cgtttttcca ttttgtatgg
    21661 cttttgattg gaatgaaaat acacttccta atgcgatgaa aaatatcaaa acgcctgctg
    21721 taattcctgt gcttaatttt aatattttta tacgattttc tttattgtaa attgcattat
    21781 cgaaaaaatc tttcaaccct aaaacaccca taatcaccat taacacttgt gtcaatgcca
    21841 atatcatcgt aggcgctcga aacttattga aaccaggcaa gaaatcatac agcaatcgat
    21901 agaaaaactc catatttttg cctaaagaaa ttagcaaagt aaaaataacg ccgccaataa
    21961 tccagccttt taatttgcct ttataggcga agcaggcaaa cacaaacaag aaaacaatga
    22021 tagcacctaa ataaattggt ccttgtacaa atggctcgct tccccagtat aatggcatac
    22081 gttgaacaaa ctgtgatgct tgctgaggtg aaacaccttt ggcagatagt gttttatata
    22141 ctgctgagtt ttcatctaag ccagcagcag aagagccacc tgcaaaatta ggaacaaaca
    22201 atgagcctat ctcttcaaat cctaaactcc agttagtagc ataatcaaag cctacacctt
    22261 tttcatgtga agtaggtgcc gatggctcac caggattcat ttttgccgca ttgagtatat
    22321 cgccaccacg cgttgtgtct ttagaaaaat cattgataaa ataattatca aaattcatca
    22381 taatggtaat agtaatcgca actgccagta aggcacttga agtggctgct tgtttcattt
    22441 cgccacgaat actttgacta attatttcga ataatgcata cacgccaatt agtataaaac
    22501 cataataagc aatttgcacg tgccgtgctg ctataatctc gtaggagaaa atcaagaaaa
    22561 gaataatgcc ccaaatatat ttgcgctgat aagtcaacca agtagcagca aacaaaggca
    22621 taaacatagc aattgcttta actttagtaa tatgtcctgc atctaagctg ataaaattat
    22681 atgtacaaaa tccgatagca atcgcaccta aaaacgcata tgatttttta gccgtaagca
    22741 cataaaatcc aataaatgca ccaagtagca ttaacataat ttcgaaagct ggatctggaa
    22801 tggcactagc tatcttatac aaggtggtgc gtggcgaatt ttctattact ttactaaact
    22861 caaggccgcc cattagatag gcaggcattc cgctaaacat tcgagaagac cacatggtca
    22921 cgtgccctgt tgttgctttt aaatctgcac tttctttatt aagcgcgatg tattgattgc
    22981 tatcgtgcgt agataattgc atgccttcca agagcggctt gcagtataaa aatgtcaata
    23041 ctaaaaacaa tagcatcacg gcaggaatca tgattttatt ggacaactga agcaaaggtg
    23101 ctgatgatga ttttgctttt tgtgctactg gttttggcgc ttgttttttg gacattatga
    23161 acgatttaag ttactaaatt acaactttat ccattcgctt ggataaatgt cgtttgaatt
    23221 tatcttgtca tctgtaaacc atctgtttgg tgcaatgact attttgtttt tgttggcatt
    23281 taaccaagcc gcccaccagc taaatgtgct attgcaaata atattatgtt tgcacatcga
    23341 cattaattgc atatccaaat agcttgttgt ggccgtatta ttattttcaa caatataata
    23401 tggaaattct gttgtaaaat ttgattttat ccagtgtaca tcatctgtaa aaataaaaaa
    23461 aactggattt tcaatttttt cagcgatgtg ttgaatggca ttttggtaat aggaaaatgg
    23521 aatttgtgca aacttattta cattctgttt taatgtaata taatcaccgc gtctaaaatg
    23581 tatagaaacg gcatttgaat tttctatttg atgttgccat gcaagatttt catttcttag
    23641 tttatttttg aatgaaaaat catttctaat tatatcttca atttctataa aatatttatg
    23701 cgactgaaaa ttgcctttga tgtagcaatt atcctttgta tgtagcaatg ctggctgaaa
    23761 atgaaagtga ccttcactta cttcgtttaa cttattggaa ttccacaaat cttttaaaat
    23821 atgttctatt ttggcaaatg gatatgcata ttttgattga aaaaaattat gagaaatttg
    23881 tgcatggata ttaaaaatat caagctcgta tctcatttgt gtagccaagt ttttaaaagg
    23941 caatttgctt tgcaaatagg aagtttgtaa gcatagcgtt gtttcatttt tgacagctat
    24001 atttcttgca aatgcatatt gaaacatttg attaccaagt ccacctgtca acgctacttt
    24061 taccatgcta tactttataa aaacaagatt ttatttgata aaagataaat caaatcatcg
    24121 atacttattt aaactttcta agtaaatgaa gtattttcaa ttttattatt ttatacaatg
    24181 aatatttatt gatagtagct gcatcgctat aaaaatcagc ataattttgc atccaatatg
    24241 tatctattgt tttttgcatt tgttttggga ccgcaacgat aacatcttga ataatggtaa
    24301 cattatgatt tgggaaatgc tgaaaaaaca acttaaatat ttcgcttatt gttggccaat
    24361 cttcagattt atgcggtgga ggcaactttc ctaaaaaaca acgagcgtcg tcaattaaaa
    24421 cgacagcatt atctcctatt ttactaatgg ccaaaagttc atcttccaaa ggacattcat
    24481 gttctttgcc acctgcgccg acacaccaat gaccatctaa ccaaaacaaa gatttatcgt
    24541 ttattttttc aataatttct ggaattctat ctttgctatt gccctgtaaa aattcaatat
    24601 ttggcttgcg gtcaggtcgg tttgatgttg atgtaaaaat ttcttcgttg atttcaatag
    24661 tgtacacttt ttcaaaataa tcggcagccc aaattgacga atcacctctg aatgtacctg
    24721 tttctacaaa gtttttaatt ttataatcat tgactaaact agtcactaat ttttttggaa
    24781 ttgaaaagct aactgcgccc atttatgtat tgttctaatt tttaaaaaat ataccatcta
    24841 tttgcaacat tcttcctgtt gtttcatcta caacgcctgt ttctatttgg tacaaaccga
    24901 agttatgttg ttttaaataa gctatgattt catcaaataa aatttcattt tcatacaatt
    24961 gaaccaatga catttctatt tgaaagccta ttattttatc gaataaagag gttgcacctt
    25021 ttagcacttc catttcaaaa ccttgcgtat caattttcaa aaatatattt tgattatgca
    25081 attctaatgt gtcaaaaatg ctgtctaatc ttttaatagc tatcgattct ttgcgcacat
    25141 attttgcatc ttgagcagct gccacatgaa tatctttaat ctcgagaaaa gagctgctta
    25201 ctgtattttg agacacattg atttctgtag tttcatcgct tgctcctaaa gcataatgat
    25261 gtgttgtcca attggaaaag cgctgtgcat ttgcttcaag ttttttgaaa gtatcgagct
    25321 gtggttcaaa agaaataatt ttacccttgt agccgtattt tctgagtttt agcgcatatt
    25381 gtccttcatt tgcgccaacg tctaaaatgc aattgacctg atgtgatgtt aataatttta
    25441 acttattttt gtaaaacttt ccaggttttt gatagatatc aaatcctaaa aaattaaata
    25501 ttttaattat taaccttttc aatatactta aattaaagca caaagaaaat aaaaaaaact
    25561 gtattgaatc gtaatttcaa caaaaaatgt cataccgtcc taaaatatca ataattacgc
    25621 ctgtttttaa taatgaaatg gaaataggtc gatgtattga cagtgtattt ggacaaactt
    25681 atactaatat agaacatatc attattgatg gtaaatcgac agacaattcc ttatcaatta
    25741 tcaaagagaa agctgcacat tttgacatca aatatatatc tgaaaaagac gaaggcattt
    25801 atgatgcgat gaacaaagga atagccttag ccaatggtga ttatctatat tttttaggta
    25861 gtgatgatgt attttacgcc aacaatgtgt tgcaggaaat agtagtgaaa ttcgagaata
    25921 atgatatcat atatggcaat gttttcttta aacatgcaca acatatttat gctggtgaat
    25981 acaataaaaa tagaattata gaccaatcta tttgtcatca agctgctttt tttagtaagc
    26041 gtacatttaa aattattgga acgtatgaca cacaattaca acttgccggc gattgggatt
    26101 tattcttgcg ttgtttttat cacgatgata taaatattag atatatcgaa aatatcatct
    26161 gtctttacaa tgatttaggt gcaagtggat acgcctcgga aaatagaaaa accaagtatc
    26221 gtgttttgta taatcgatta ggaattaatg gaattctaaa gtatgttagt attcaaatca
    26281 aaaagaaatt atcaattcct ttttattaaa gaaataaatt cttttgtatt aaaaatacca
    26341 gttgaataca aaatccaatc tggtaatttt ttgtgataaa aaggttcata aattataatt
    26401 gcattcgata aaattgctgc tgcatagcta tatgcacttt tggaagtcac taagatatca
    26461 gcatttacca aatcagaaaa atctgaaaac acatcaccat tcagatgtaa ttgtgctact
    26521 gtatctaagt tgtcaaaatc attcttattt ccttgagaat aaatagaaat agaatacgat
    26581 aattgttgct tgtcgagcat ttcgcgcaag gctataatct tatcgtatat ctctgaatta
    26641 cttgtaaatc tctcgttatt tgcggttgca gacacatcgc cacgtctaac atgaaatatc
    26701 acttgtaaaa tttcattatt gtgatttgtt tgctgtttat tgtgataata tttatttctt
    26761 agtcttgctt taatatcagt aaattggtgt gtatgcagat ttgcataatt atgaaaatga
    26821 gatttaacaa ataaaaatga gtttccaatt cctaataaat atttcgctgt atcaactatg
    26881 gttcgccaag aaaaattatc taaataaatt gtagtgctcg atgtataaaa ttcatctgca
    26941 ttttgttcac ctaaagaaag tgaaaagaaa tcttctactt gagtgttaaa attttctgta
    27001 ttattataat tatgttctac tgaatgaatt ggtgtatggc aatacttaac acctactgca
    27061 tttgcataca acatggctga ataaattgct tgcacttgtg cgcccattcc gtctgttttt
    27121 cctgcacaag tgattgtaca tttttttcta attggaatta gtaccaataa aacgatatag
    27181 gaaaatgaca atgcaaatag ttgattaatt ttcttcattg tattaatgat gttgtcaaat
    27241 ataattcaac agtatggttt atggttttag taatgattta acttgaatta ttttatcgta
    27301 aaaaacataa actactaaaa ttagaaacag aataattttt gtagcaatca atagatataa
    27361 ttgttgaata aactcgggaa gcacaaatga tattagtgcc atagcggcaa tcaaaataat
    27421 actttctaaa aaaactgtcc aacgtggcac gctcagctgt gtataaaatt tgcacactaa
    27481 ataggtagta gcaaataaga ccataaaaga aaataaagtt gtgtaagcag caacttcata
    27541 gccatatatt ggaatcaaat aatagtttaa agtaaaattg attatagctg ttgttataaa
    27601 actaacataa aataatttta cttttttaga gtagtgtaag aagaaattat agacttcagc
    27661 catatcagaa aagacataag ctaaaacaag cactggcaat aaatgtaatg catgttcgaa
    27721 ttcaggcttt aaggataaaa tccttccagc atctccagca aatagtataa aaacgactga
    27781 taataccgtt attaatttaa acatactacg catttgtgtg ataacttctt ccgatttatt
    27841 ctcatttaat aaattaaata aattagggcg attggcagtt acaaatgctt gagaaaatcc
    27901 cgaatatatg acagcaaatt tataggcata actatatacg ccagatgctg tgctgccttg
    27961 catattattg ataagtacta aatcaacata actaacaata taactgctga gtccaatggg
    28021 aataaatcct gcagaatatt ttaatatctc ttttttgtca ggtaattgtt cttttaagcc
    28081 aatataattt ctgaaataag aaaaaataat gagcaatgat gctatgagca ataaaatatt
    28141 ttcaccagca attcttccat aataatctct actaatataa ataacaaaaa taatggcgag
    28201 cactactctt cctatttgcg aaactaattg ccatatacct gctcttgtgc tatattgttg
    28261 tgcattatta taggtcgtaa aaaaattgaa aattatagtg gaaaaaatac caattggaat
    28321 ccaccaaaac aaatttgaag gcaggcccgt tattttcaaa acttgtgatt gtaataacaa
    28381 caatactgcc aagccaataa taaaagacaa gagcgctgta cgtagtaaaa attgcatgag
    28441 tgcattttta ctgatgtttt cttcgaaata aaaacgataa aaaccagtgt ataaattaac
    28501 agaaataatc actgctaaaa agcgaactgt gttattatac acttcataaa taccaaaatc
    28561 tgctggtgtc agtagtttag taaaaacagg tatcgacaat accattactg cttgcaccac
    28621 tacaaaagac aacatataca ccgagccatg tcttagtgta ttataaaact gcaattgctt
    28681 tatatgttgt ataatttttt tcaaatattg gttctacaga tgctatttaa taaaccattt
    28741 tggcggattc aaataaatgt cttccgtttt tacatttgtt cgccagtgct taggtataac
    28801 tattttttta ttaggatttt tattcaacca agctgcccac caactaaaag tgctattggc
    28861 aataatattg tgtttgcatt gactcattag ataaaagtct tctatcgtag aatttcctga
    28921 caatagtgtg caatcaatat cccaatgcat ctcgcgatgt atccattctt catcatctgt
    28981 aaataaatag aaatgtaaat cattgcctaa ttcgcgttta ataaattcaa tggcttttgt
    29041 ataataatct ttcgaacaca aaccatgcgt atccatagca gataaattac gaatataatc
    29101 accacgacga atatgaatag aaacagaatt tttatttgaa atatcttgca ataaatctgt
    29161 tttttgagga agaaaattct ctttgatgac taattcttct tttaaagatt ctgcaatatt
    29221 cttgaaaaat ttctctgatt ggaaaaatcc tttcaaataa taatttcctc tcaattctaa
    29281 aacattacta tcaaatccat attcattaaa tttgtacttg ccatatttcc caaacgacaa
    29341 aagtttagtc aacatgattc ctgaagttga gaaatcattt aatttaaaat gtttcaataa
    29401 ctcctcatta gcaagtgttg cattgatatg aaaggctggt aattcatatt tccatttcga
    29461 atcaactgta ttatctgaga atgcgctagt atcaatatac agctctgttt tattcaaatg
    29521 cgctacatgt ctgcctgtag catattgaaa aagttggttg cccaatccac catttaatct
    29581 tacaactatc attataatat ttgcgtacga atttgtattt tgctgtaaat ttacaaaaat
    29641 gttattcatt ggtacaggaa aaggagatat tactgcattt tttaaaggca taggcatgct
    29701 tggttatggc cttccgcaac acattatgct tgatattgaa acgccaatgt atgcacgtgc
    29761 ctttgttttt gagcaaactg acaaaagtca aaaagtgtgt tttgtgaatt gcgagctggg
    29821 atttataacg ccttcattaa agcaaggcgt tcttaatttg cttactaaag agcatccagc
    29881 actacaatac aatactagta atttattatt gtctgcacaa catacgcatt gcggaccaag
    29941 cggattttcg catcatgtaa tttataattt atctactcca ggttttcatc aaggtgtata
    30001 tgacaaaata gtaaatggca tagtgcagtc tattattgaa gcagaaaaca acaaacaaaa
    30061 agcctctatt caaattggaa agagtgcatt ctcaacagat attcctgtaa gttttaatcg
    30121 tgttattgat gcgtataatg caaatcctga agtgacaaaa ctaacacaag aaaataggca
    30181 tttagcagtc gatagagaaa tgaccttatt gcattttata agtgaacacg gacaggcatt
    30241 aggtagtgtc aattggtttg cggtacacac atcaaacttg ccaaataact attacaagct
    30301 atgttctgac aataaaggat ttgctgccag ctatttagaa gatagcatga aaaaagaaaa
    30361 caaaaattat gtagcagcat ttgcgcaagg tgcatgtggt gatgtttctg cacgtgtaaa
    30421 atacaataat aacttacctg caacacgtgg taaatgggaa ggatatttcg aagatgattt
    30481 aaaaagcgct aagtttaacg gcaatttgca atttgaaaaa gcaaaagaaa ttacacataa
    30541 cgcattagaa acaattaagc aagacagcat tgattatatt tcaatgtatg tagattttag
    30601 caatattcaa atagactcaa catttacaaa tggaaaacaa gattgcgtta caagtccttc
    30661 gtgtatgggc gttgctttct tagaaggctc gatcatggac gggccaggat taccacaatt
    30721 tttaggaaaa atagctagag ttgttgcgaa aagaacgcgc aaaaaagaaa ttaaagatgc
    30781 tcaaaaaata ggtggcgaat atgctcaatt tatcgctaga aaaaacaaag cgcaatctgt
    30841 taaagatatt ggtgtagcaa gtggcgaacg tagattttta ggcaattttg atgccgataa
    30901 attgccattg cctggatttg ttgacgaaac aattttaaat atcaaaaaga tggcaaaaca
    30961 aggtggattt tcttccaaca atccttgggc tcctcaagtt ttgacattgc aaattttaaa
    31021 aattggaaat ataggaatct gtggttttcc ttttgaaata acaacagttg ccagctggcg
    31081 attaaagaaa acattagcag atgcattgca aagcaaaggg attgattttg tcatcttagc
    31141 gccatactct aatgaataca atggctacat taccaccaat gaagaatacc aattgcaatt
    31201 gtatgaagct ggacacaacg tgtttggaca atggagttta aatgctttac agcaaaaatt
    31261 ttctgagttg gcaacagaat tttgtaaaaa ccaagaagat agaaatacag acaagcatgt
    31321 acatccagaa ttatttacta gtgatgaatt gaaaaagcaa atctattttc caaataagac
    31381 tgtccaaaaa ctaaataaaa aataaattga ttacatttaa gatgtcaatt taatactaca
    31441 atttttatgt atgagtaaaa tatttcaaaa tgctacagaa gcgctatatg atgtaggcga
    31501 caatatgacc attatcttag gtggttttgg cttatgtggt attccagaaa acagcattgc
    31561 tgcattagtg aaaaaaggag tgagtggttt gacctgcata tctaataatg ctggcgttga
    31621 tgattttgga ttaggcttgc tattaaaaaa tcatcagata aaaaaaatga ttgcttcata
    31681 tgttggtgaa aatgctgaat ttgaacgaca attattgagt ggtgaattag aagtagagct
    31741 aattcctcaa ggaacattag cagagcgctg tcgggctaca gcagctggta ttcctgcatt
    31801 tttcacacca gcaggctatg gtacagaggt tgcagaaggc aaggaagtac gtgagtttga
    31861 tggcaaaatg tacatcatgg aaaaacgttt tcatgctgat ttctctatca taaaagcatg
    31921 gaaaggcgat acaaatggca atttaatttt taaagcagct acacgcaatt tcaatttacc
    31981 aatggctgga gcaggaaaaa ttaccattgc agaagtggaa gaattagtgc ctgccggcga
    32041 attagatcca gaccaaatac acacacctgg catttttata aaacgaattt tccaaggtgc
    32101 gcattttgaa aaaagaattg agaaaataac aaatcgcaaa aaataaaaaa caagctatgc
    32161 tcgacaaaaa tggaattgca aaacgcatcg ccagagaatt acaagatggc ttttatgtca
    32221 acctcggaat aggtattcct accttagtgg caaactatgt tcctgaaaat atacatgtaa
    32281 catttcaatc tgaaaatggc atgttaggca tgggtccatt tccatttgaa ggcgaagaag
    32341 atgcagacgt tatcaatgct ggcaaacaaa ctgtaaccat gatgcctggt ggcgtctatt
    32401 tcgactcggc tactagcttt gcgatgatac gtggtgagca tgttgacctt accgttttag
    32461 gtgcaatgga agtaagtgaa acaggtgata ttgcaaactg gaaaattcct ggaaaaatgg
    32521 taaaaggaat gggcggcgcg atggacttag tagcttccgc aaaaaatatt attgttgcca
    32581 tgcaacacgt caataaagct ggcgaaagta agctattacc aaaatgcaca ctgccactga
    32641 ctggtattgc ttgtgtgaaa aaaatagtaa cagaattggc atttctcgaa gtgacggccg
    32701 acggttttaa gctgatagaa cgtgcaccag gcgttagcgt agaatacatt caaaaggcca
    32761 ccttaggcaa gctaattata gaaggagaaa ttcctgaaat ggtgatataa aaaaactaca
    32821 gtaatggcac atctataaac aacttcacgt tgccatctgt accttttgaa cgatttttca
    32881 ttgcttctaa tttctctaca tcattgcaaa taattaagaa tagatattca tcattcaatg
    32941 cacttttctt agtttttgca tacggatcgc cagcatacga gttcaagttg gtaaccttgc
    33001 atttataatg cttgttgtaa tgtgatgcta ttccttttaa caatacatca acattttctg
    33061 gtgagccagt tatcattatt ggatacaaaa tattatccgt caattccata tttgcaacag
    33121 ctttaaacaa gccttgcgga ttttctaatt ggaatgcaaa atattcgccc caattgatag
    33181 caaagcatac tgcatccata ttgccattac tatctggcac catatataca cgtgatgatt
    33241 tagaatatgc aaaaatgcgt tgtaaagtat ctataaaatc ttcttccgat tttaagtgat
    33301 aaaaagtata atgttctttc tgtgctttta atttatggta aaattgtttg agcttttcta
    33361 catcatctga aatatctatg cactttttaa aagcatcttt actaccaaaa aacttattaa
    33421 cttttgtatt gtttgtgaaa gcccattttt catagtgttt ttcatacaat ttagtattga
    33481 gttctaatgc ttcatttatc gcttggtttt cttttcccat tgcaccatac gtaaacgcaa
    33541 tattttcttc aacaccaact atgctatgca taatgctaaa cattagtgta aatatcttct
    33601 tgcgtctata cacttttcta ttctcttcga cgcgttcgct cacacgcaac atattgtaat
    33661 aatatgcatc gtaacgttga ccttcaagat atacgtccat caagtttaca tgtatgtatc
    33721 cgactacatt aaactcgcgt tttccatttg catctaattt ttgtggatgc aaaacgataa
    33781 ctactaaagc atctttatta atagacaaaa ctgaattagg aaaattatga aagtaagctt
    33841 ggaatgcaaa tttaccttga cctttgttgt tgatatgttt ctctggcact gaaataatat
    33901 ccttgcattg atgaaagaag tatccagtat caggcacata acctaatgta tccaatatat
    33961 ttcccatttg tggtaagaaa ttaatgtctg taacaataat ttctaatggt tcttcaaaaa
    34021 tatcatagga atcaatatcg atggttttat aggcaaatga accatctgca tttttttcta
    34081 ctcgcttata atttctctgt tgcatactat aaattgacat gcaaatttag acttgattat
    34141 tgatgttccc tattaatgtt tgtaaattta ttaatgcatt aacgctcatt tatttataat
    34201 cttaaaaaat accaatttac aagtaatcgc ttgtaaggat aatcgcaata ctaaaaacaa
    34261 aaaatcaatt ttgattatac aataagttta tactgctttc ctggcaaact ttgaattaca
    34321 ccttgcattt ctaattgcaa taaaatactg gaaagctttc cgagtgtaat aggcaggctt
    34381 gcactcaata catctatatg taatttgtcg gcatttttaa gcatatctac tattgcttgt
    34441 tcttgctcat taagttctaa aaataattgt gtttgctgac ttggttttgc tgttgtatca
    34501 tcattggcaa tccaattcat actttgtaat atatcatcag catttgttac aagttgcgcc
    34561 tttaattgtt ttatcaattt aagacaacct ttggaatagg tatccgtaat ttttccagga
    34621 taagcaaaga catctctatt atatgaattt gcaatttcag cggtaattaa tgcgccacct
    34681 ttttccgctg attcgacaac aattgtagcg tctgtcatgc ctgcgacaat tctatttcta
    34741 ttcggaaaat tccctttatc aggaatagtg ccactgaggt atgttgttac taatgcgcca
    34801 ttttgttgaa gccgttctgc gatgtttttg tgttggtttg gataaattat atctaaacca
    34861 tgcgccagta cagcaatatt ttgtatatcg tatttcaagg ctgatttatg ggcacaaatg
    34921 tcaataccat aagccagacc actcactata tgcacttgat gctgagccaa aacatttatc
    34981 aaattttcag ttattgcttt accgtattct gtcgcatggc gtgtgcctat gatattgaca
    35041 aatttatctt tattaaaatc tacatttcct ttactaaaaa tatataaagg tgcatctgcg
    35101 caatgtagta atcgttttgg ataacgctta tcgtaataaa acaagatttc aatattattt
    35161 ttttccacaa aagccatctc tcgctctgct attttttgtg cttcataagt atcattaaat
    35221 atggatgcta attttggacc gatgccatta attttttgca atttggtttt ggacaattta
    35281 aatatattct cagcgctacc tgcataacta atcaatattt ttgcatatgc atcgcctatt
    35341 cctttcaact gactaagtgc aatgccataa aataattctg tactcatctc ctattaagag
    35401 acaagacagt atcaaattcc ataaaattga aagtaaattt ttagaaaaaa attaaaaatc
    35461 tgtgtagcga ttaaacatat tcgtgcgcaa gctgataccg aaatataaat tttggcggct
    35521 ttggaattta tttttcaaat atctatagtt tacaaatgct tctacagata gatttatttt
    35581 aggattaatc aaataaccgc ctcttgcttc tacgttcact aatcttgttt ttacaccttg
    35641 caagaaataa ttgccatatg cagttcctaa atttggcgtt atcataaagt cagaaataaa
    35701 aatattactg ccatcatgtt caaacgctgg gtctaagcct tgcacaacat actgtaattt
    35761 tgcagacgct gtccaatgat tcttccaaaa atttaagaaa gtaatcgact ctagaaagtt
    35821 ggcgcccatt ggatgtgtca gagattggtt gaagttggta taactttgct caggtgcttt
    35881 gtgtgcatac acaaatggtc gcacaatatt taattctgtt tggaaatcga gatgtttaac
    35941 tttaaataaa tcatagcctt tataaccaaa ttgtgcagct acttttgtgc ggtaaaatcc
    36001 ttttcctttt cgtgctgcac caacgtccat atcatctaac atgatttgag tataaaaaat
    36061 ttgttttggt gaagctttga acttcatacc aataccaagc accacgttat ctggcgaacc
    36121 gagtgcaaac tctacagggc gaataaatgc aattggaatt aaatagctga actcaatacc
    36181 tctaatacga ttggaatcat tatgaaacca agtaacacct tcaaaaaatg tgaattgcat
    36241 ccattttgcg acgtcaacag aaattaaatg gaaagtacca tatttagttc taaagttcga
    36301 aggatttcgc tccacattaa aaccgtcaac gggtccatag cgcatcacgt ttaagagata
    36361 tacatatttt atacgccaaa aattagttgt aagctttaga tatggactat tcgaagcatt
    36421 atcactcaag aaaacggaac ggtaaccatc gccccaaaaa tttttatcat tgccaacttg
    36481 taagttaaaa tatttattag gcgaataaga aaaataacct gagaataatt gcgaattaat
    36541 tcgattacct ttaaaagttg ctttgcgata gccaggcaat acgccgtatg tagctgcata
    36601 attgctgact tgctgtaaat aattttctgt aacaccttga taggtgaatg acaaggagac
    36661 ttttttacct aagttggcat caaacttaat tccgtaaccc gctgtatatt gtaatttctt
    36721 ggcaagcgga tcgtagccaa cttgcatatc taaaacagga ttaacagcga aataaaattt
    36781 atgctttttg gtagcgacag agagcatatc tccttttagc aatccaaatt taaaaccatg
    36841 tttgctggag tctgtaacat aacctaattc ctgcaaagca gaatcaccga tggctgcttt
    36901 aacttcagct aaattataag gcttcgcaga agtaataaaa tgcgatgaac tttgtatata
    36961 acgttctaag cggttttgca tttcataatt caacggaaag tttaactgct gtgcaagtgc
    37021 tgaaacggca aaaaatacaa atattacgga aattgctttt ttcattggaa ttctgtctgc
    37081 taaaataatt gtttaaacta taataaaata gaatagcgca aaaaagttat gggcaaaagc
    37141 taaattcgta cgattctaag ctgtaaaata ggaattaatc atgatttcgc taaaaaagaa
    37201 cataaaacta atcaacttct atcctccttt tttaggtgct ggtatttctc taaaatctgt
    37261 aaatgaagat tttaccaaaa ttgaagtgca gttaaaaatg cgctggtata atagaaatgc
    37321 tgtaggcact catttcggtg gctccttata cagcatgact gatccatttt atatgtttat
    37381 attaatggaa aatcttggaa aaaattacat cgtttgggac aaggctgcct gcattaaatt
    37441 caaaaagcct ggtttaaaaa cagtaagtgc tatttttgaa attaataaag atgaaatttc
    37501 aaacatcaaa caatatgtgg acacgaatgg caaaggtgat ttcactttta ctactactat
    37561 ccgaaatgaa gaaaatgaag tagtcgcaga agttgaaaaa atagtgtatg taagaagtaa
    37621 aacatacagc aacaattaaa cttcatttat tatattttct tgcgtttttt cttaaaaaat
    37681 gaagttaata atgctgctgc ttcattcgct aaaattccat tttttacttc aaataaattg
    37741 ggcgctgcac ttcgattcga ggtgtctgct gctgcaaaaa cgactttttt aatttgcgat
    37801 tcgcgcaagg cacctgtgca catcatacaa ggctcgagtg ttacataaag tgtgcatttg
    37861 tctaaatatt tactacctaa aaaattacac gcagcagtaa ttgccaagag ttcagcatga
    37921 gccgttgggt catttagttt ttcaacttga ttataacctt tgccaattat tttgtcgccg
    37981 caaactacca ctgcaccaac aggcacttca tcttcatcaa atgcaatatt agccaatttc
    38041 aaggcctcac gcataaaaaa tgtatcaaaa tccaacattc ttacttttta ataattgaag
    38101 atagaacttt tagctgtact tttatgttgg ttgtaagcgt gtaatatttt acatacattt
    38161 accactaata gacaaaaata tatttcgtta gcatgaaatt tagttacaag cgtatttttt
    38221 tattgtttgg aatagccatt gtttacgcaa atggtatagc gcaagaaact tggaattggg
    38281 aaaaatgcat tgactatgca ttaaaaaaca atcttcaact tcagcaagct gatgtaaaca
    38341 tcgcattagg tgaggcgacc cttaaaggca ataaaatggg atattcgcca atgataaatg
    38401 ctgatgtcaa ttacaactta agaattggta ataacttcaa cttctttaat agttcttatg
    38461 aacgtcagct tgtgcattat caagattatg gtgttaatct atcacaacca ataatagact
    38521 tggtaacgcc tgcaacagta aaaaaaagta aattagaatt aagcgcatta aagctcgacc
    38581 aagaaacatt aaaaaacaac atacagcttc agttgcttac tgcttttttg aatattatga
    38641 atgccaatga acagaaagaa caagcaacac aacaacatca aacgacgaaa gagcaattcg
    38701 atagaatgaa tactttaata gcatctggtg cagcggctga aaatgcaatt ttagacattg
    38761 aagcgcaact cactaacgaa gcgctctcga tttcacaaat caaaaatcaa ttagatatcg
    38821 cctatctaaa cctaaaaatg ttgttacaac ttgaccctaa acaatctata caagtagaaa
    38881 tacctaggat acctgagctg ctaacgctag atgcaattgc tgatgtaaat actatttatg
    38941 aagctgcatt aggcttacga cctgaaataa aaagtgcaca attaaaagca cgaagtgcac
    39001 aacaacaaat caatgttgcg aaaggcaatt attatccttc tttaaatttt attagtaatg
    39061 taaatacttt ttttacaacg caaaataaaa gcagtcaaac tacaacaaca ggaaattttg
    39121 tgccttcagg tgcaattgtt gatggtacat tccaatctgt attaattcca gaaacaatta
    39181 ccacgcaatc taaaaatcca tataaaaagc aactgaatca aaatttaagt tatgcatttg
    39241 gtctaacatt aaacgtgcct attttcaata agtttcaagt gcaaaccgct gtaaagcaat
    39301 cgcgattgaa ttataaaatg gcattggtaa gtgaaaagca agcaggctta gatttattta
    39361 attcgataca acaagcacat ataaaagcac aagctgccat cgaaaattat actgctgctg
    39421 ataaaaatta ttcgattgca caaaagtcat acgattatgc attggaaaga ctacatgctg
    39481 gcagcatcag ccaattagaa gtaaacaccg ctaagaataa tgttgccatc gccttatcta
    39541 aattgacaca atcgaaatac gaatatttat ttaatactaa gttgctagat ttttatcaag
    39601 gcaaaaaaat cgaactataa cataaaccaa tagcatcaaa ataaaagaag atgaaaaaca
    39661 ataaactgct atatattctt ggaatcatta catttatcgc attgttagct gcctatttta
    39721 ttggaaaaaa cagaaaagca aatgcagcaa aagaagtgga atggcaagcc gtaagcacgc
    39781 gtacaattat acaaacagta acagccagcg gaaaaatata tccagaagaa gaagtaaaaa
    39841 taagttctga tgtatctggc gaaatcattg aattgtatgt aaaagaaggc gatagcgtgc
    39901 agcgcggaca actattagct cgtataaaac ctgaaagcta tcaagcaatg gttgaacaaa
    39961 gcgtagcaca gctcgacaat actaaagcac aattatctac tgccaaagca agaatagctc
    40021 aagctagtgc cgtattgctg caagcacaag cacaattcga tgccgcacaa ttagcctata
    40081 cacgtgcaca agaattattc ggtaaaaaaa taatgtcgaa agcagatttg gaaagtgccg
    40141 aatcgagttt taaaacttca aaatctgcgg tagaaagcgc aaaggcagat gttgacgcag
    40201 caaagcataa ttcagatgca gcgacattta caattagagc aacagaagca accttaaaag
    40261 aagcaaaaac aaatctaaat aaaacaagca tatacgcgcc aatgagcggc atcgtttcct
    40321 tgttaaatgt aaaaaaaggc gaaaaagtgg taggtacttt acaaatgaca ggaacagaaa
    40381 tgatgcgcat tgccaacttc gacaatatgg aagtgcgcgt agatgtgagt gaaggtgaaa
    40441 tcacaaaggt aaaattaaac gacacggcaa gtattgaagt cgatgcctat ttagacagaa
    40501 aatttatcgg cattgttaca caagttgcca acacgtctaa aggagctggt tctgctttag
    40561 taagcgctga tcaatcgaca aatttcttag taaaaatcag gctattaaaa tcgtcttacg
    40621 aagatttatt agtagaaaat ccaaaaccat ttttgccagg catgtccgca acggttgaca
    40681 tcaaaactaa aacaaaaaca ggagttttgg cggttccaat ccaagctgtt accactaaag
    40741 atacggtgta tgatggctcg agtcatacgc gtgaaattat tttccttaaa caacaaaata
    40801 ttgcaaaagc aatagatatt aaaactggca ttcaagatga tgcatacatc gaagtaacaa
    40861 acggattgca aggcaaagaa accgttatcg taggtccata taatgtgctg gtgaaagaat
    40921 taaaaaatgg agataaagta aaattattag acaaaacaaa aaagaaagaa gacaaagaca
    40981 aagaagaaga tgaggataag aaataacaca aagtcatcaa gcgccaagtt gcaataattc
    41041 tatcgctttt ttgcaagcta attgttctgc ttctttcttg tttttaccaa ttgctgttgc
    41101 tcttacctca ccttcgataa gtaaggcaac ttcaaacaaa ccattttcat ttacactaga
    41161 tttatgctca aattgcaagt gttttttttg tgcttgcgac cattctaata aataactttt
    41221 actatctgta atagttttat gaaaattatg cgcatcaaat tctttcatca gtaaggtcgc
    41281 aataagcttt tccatttctt tcatgccata atcgatataa tatgcaccaa ttagcgcttc
    41341 gaaagcatta ccaataatat caggtgcttc ctctaattgt tttggcaaaa gtttatgttt
    41401 gagatatttt ggaataccaa tatctacacc tactttattc aaattttttc tattcacaat
    41461 agatgcgcgc gctttggtca tcgtgccttc atctttatta tgcatttgat gatacaatac
    41521 ttcggcaaca caaaaattaa ttactgcatc accgagaaac tctaatcgct catttttttc
    41581 ttccattttt ttggtaaaag aagaatgcgt caatgctcga atataattgt cttctgattg
    41641 caatggatat ttatttaagt gctggtaaat atctataaaa ataaatgaag acgaagtcgt
    41701 tgcaggtttt gagaatagtt tcttaaaaaa tgaaatcata caaacaaatt tagctattag
    41761 ttatgaaaaa aatagctgaa aatttgaaac aagattatct ataaagatga atggaatttt
    41821 ttaaagacaa tagaagcgtt gtggccacca aaaccaaaat tatttgacaa tactgcatgt
    41881 atttgtcgtg cttgtgatgt atttaaagtc agatttaaac gcgtgtcaaa tgctgaatca
    41941 tgcgtaaaat gattgatagt tggtggcact ctttgtgttt gcagcgacaa cactgcgata
    42001 acggcctcca cgataccagc ggcacccatt aaatgaccag tcatagactt tgttgcacta
    42061 atatttaaag aataagcatg ttcaccaaat aattgttgta tcgcttttat ttcagcaata
    42121 tcacccaaag gcgtcgatgt gccatgtaca ttaatataat caatcgcatc agttgatagt
    42181 tgtgcatctt caatggcctc ttgcatcaca tgtattacag aatcgccatc tggatgtggt
    42241 gcagtaatat gatatgcatc agcattcatt gcaccgccag cgagttcagc ataaattttc
    42301 gcaccacgtt tttgagcatg ctctaagctt tccaacacca tgcaagcact accttcgccc
    42361 atcacaaaac catctctgtc cgcatcaaaa ggacgcgaag ctgtatgtgg ttcatcattg
    42421 cgctctgaca gtgctttcat cgcactaaat gcgccgatgc ctacttcggt aatcgatgct
    42481 tctgcgccac caacaatcaa gacatcggct tttcctaaac gtatataatt taaagcatca
    42541 atcagtgcaa tcgttgagct ggcacaagca gcaactacag aataattcat tcctttaaat
    42601 ccatatttaa tagaaatggc accacctact aaatcaagta aggtgcgtgg cacaaaaaac
    42661 ggactgaaaa atcttggatt ttttccattt tggtagtatt gaaataattc ttgttggaaa
    42721 gtttgcacac caccaaagcc tgacgtaaaa agcacaccaa cacgtctttt atttagcgat
    42781 tcataattga gaagctgagc atcttgcatg gcttcttcgg tagctactaa tgcatattga
    42841 gcaaatgtat caaggcgttt taattccttg ctgtcaaaat gtgatgctgg tgaaaaatct
    42901 tttacttcgc aagcaatttt ggtacgaaat gaagtggtat cgaaatagga aattggagca
    42961 caagcactat gtccttcaaa taaacgttgt tgaaattgag aaatcgtatt gcctaatggt
    43021 gtgatggcac caatgcctgt taccacaaca cgatttagtt tcatatacaa gatttaaata
    43081 aaaaaatatg gtaaagagga attctttacc aataaataac aaaacttaaa tttatgcgtt
    43141 cgcttctaaa taggcgattg cttcaccaac agtttttatt ttatctgctt gctcgtccgg
    43201 aatgatgatg ttaaattctt tttcaaattc cataatgagt tcaacagttt ctaatgaatc
    43261 tgcttttaaa tcattttcaa aacttgcctc aggtgttact tcactgtctt cgacgttcag
    43321 tttatcaatt ataatttttt ttactcgttc tgcaatggtt gacatagtca atgtatttat
    43381 aaaattgaag tgcaaaatta gataaatttt tataatctgc ctattgccga ctcaaaatta
    43441 atttacacac aaaatactaa taatcaatta gatacgaaat tacacttaaa aatgttgaaa
    43501 aacagcataa ataaacaagt tttccatact taaattgcaa tcactttatg gcaagaaaaa
    43561 atttaataca agcatctttt aatttcgata tttggggcat taattcaagc atggaagatt
    43621 atcgactttg tttgcatata aacgaatttt taaaatggca actaaagcgt gtacacgatg
    43681 ttgagatttt tgtaaatgat gcgaaaaata tcaaatattt tagtgcatat aaatataata
    43741 atgacatcga cttgtacact gtcgagctta tacaaaataa aaaattaggc aacatattaa
    43801 ttccagaatt aaaaaatatc gactatttat ttttatttaa tggagaagac gattattttg
    43861 ataaagatgc ctttataaat tcgttattaa attcaccagg tgtacaatct atatttccaa
    43921 tagatgtaaa tttgctgaag tcaagacata atcttttaac tcgacacttt aatgagccaa
    43981 gcaatacgta aaactaaaat agttgcaacc ataggacctg ccagctacag ctacgaaaaa
    44041 ttagtagaat tatacaaaag tggtgtcaat gtttttcgct taaatttttc tcacggcaga
    44101 catgacgacc acgaacaagt cattcgtcat atcgtacgat taaacgaagc ctatcatgca
    44161 catgttagca ttttggcaga tttacaaggg ccaaaattgc gaactggcct catgcaaaat
    44221 aatgcaatcg agcttataga aggcaatagc atcaatgtta cctgcgaaga agtaattggc
    44281 acaaaagagc ttatttcggt tacatatgaa catctatcta ccgatttaaa aataggtgaa
    44341 ttcattctga ttgatgatgg caaattggaa ttacaagtag cagaaatttt agatccaaaa
    44401 acagtacgct gtgtagttat ttcaggtgga attttgtctt ctaaaaaagg atttaattta
    44461 cctaacacaa aaatatcatt accaagtttg acagaaaaag acagaataga cttggctttt
    44521 attctcaaac aacctgttaa ttggatagcg ctttcatttg ttcgaacagc aaaggaaata
    44581 aatgaactca aagacattat acaagaacat aaatcatcat taaaagtagt tgcaaaaata
    44641 gaaaaaccag aagcagttgc caatatagac agtattatta aagctactga tgctgtaatg
    44701 gtggcgcgtg gcgatttagg cgtagaagtg gctatggaag aaatgccatt aattcaaaaa
    44761 aatatcatag agaaaagcat acaaaatgcc aagccagtaa tcattgcaac gcaagtaatg
    44821 gagtcgatga tagacatgtc gcgaccaaca cgtgccgaga ttacagatgt ggccaatggt
    44881 gtgctcgatg gtgctgatgc tattatgctg agtggcgaaa cttctgttgg gaaaaatccg
    44941 gcgaaagtag tgcaaacgat agacaaaatt attagacgca ctgaacaaga agatgaaatt
    45001 taccataaac atctattgcc agataaatca tcaaaaacat ttttatctga tgctatttgt
    45061 tttaatgcat gcactattgc ttctgaagtt ggcgcaaaag ccataaatgg aatgacctat
    45121 tctggttata ctgcttttat gttagcgtgc aacagaccta aagccgatat acacattttt
    45181 acaagtaata aaaatttgct caacacctta gggctcgttt ggggcgtcga tgtgttttat
    45241 tataataaat ttgttagcac agacgaatct attgacgata tcattgacat attgaaaaag
    45301 gaagatttgg ttgatattga tgacattatt gtaagtacag caagcatgcc tatcatggga
    45361 agaggaagaa ctaatatgct caaaattagc aaaataaaat agctaaatcg tttggcgctt
    45421 tacttcgtaa agcgccattg ccgttgcaac agacacatta tacgattcta aagcatcatt
    45481 cataggcaat ttcaccaact tatcaagcag gcgaattact ggattcgaaa ttccttcatc
    45541 ttctgatcca gcaacaatag ctaaaggcat ttttgtattt acttcgaaaa taaacgaaga
    45601 tgcagtacca tctaatccaa tgagatgaat accattattc ttgatgtctt ttattgttgc
    45661 aattagatta tccactctac ataatggaat tttcagcaat gctccagccg atatttttac
    45721 tgcaacatca ttcacagcag cactctcttt ttttggaaaa atgatagcat gcacacctaa
    45781 tcctaacgct gtacgtgcga gtgcgcctaa gttgcgtatg tccgtaacat tttctaaaat
    45841 caataataat ggatcttcgc cattactaaa aaccatgtca attatatttt gtacttcttg
    45901 gtattttatt aatgctgtaa atgccaacac accttgatga ttagctttcg aaagactgtt
    45961 tatttttact tcaggaacaa attgaaaata tacttcattg tctttgcaaa gctgaatgat
    46021 ttgttttatc acctcgcccg atgcagattt tgacagcaat actttttcta cttcttgctt
    46081 agattgtaat aattccaata ctggatttct gccggcaatt agttgttgtt tcaatttatt
    46141 tttaaacgtt gtagcattat taatgatatc gtgtaaagat aaataaaaaa agctgcctcg
    46201 ccaaagacga gacagcccaa gatagttcta gaccaatggg gttctcttga acctattttt
    46261 tcaataaatc tcttatttct gttaacaata cttcttgcgc tggtggcgca gctggcgcag
    46321 ctgcttctgc ttttttcatt ttgttgatag cttttattac gacaaaaaca accaatgcta
    46381 cgacaagaaa tgtgatacat tgagagatga aatcaccata tttaacagct acttccgcaa
    46441 ccgcaggcgt tgcaccatct gctgatgctg gcaccgcatc tttaagaata attttctgtt
    46501 gtgtaaaatc tacaccacca gaaatcattc caattgctgg cattaccatt ccatcgatga
    46561 atgtagaaac aacttttcca aatgcgccac ccattaccac accgacagcc atatcgataa
    46621 ggtttccttt aatggcaaat tctttaaact cttttatcat tcccatattg tagtattctt
    46681 agctaattta caaaataaaa ttcaaatatc ctacctaaat aattgcattg gtatttgttg
    46741 tgtagtatct aatgctgctg ttggtattaa gattggaaaa gcttcgttta attgcttctt
    46801 gaatgtagca tctttataca ttaatgctaa gatatccatg cgttttacta attcctctgc
    46861 tgacttcttc tcattagcat ataaagcggc aaaatcatta ttaggtaaat tataaaaacg
    46921 cagacaatct tttaaatctt taataaacaa gtctttcaat tctttagctt tatctggcgc
    46981 accagctcta tagtataagt ctatccactg cacacttaga atattaaaga atcgattatt
    47041 cggtgaataa tatggtgcgt ttacatattg gaattccttt ctagcttgtt ctaatacttt
    47101 tacagcatct tcttttctat tgtttctaat taaatcatcg gcaatgctta gatgtgcgct
    47161 tttaagtgtg ttcataattc taccagagtt ttcatcgaca aacatttcgc gttcacctaa
    47221 tccaccatat ttgaatttct tagtgacaat atcaaaactt ttctgcgtgt tcatagcaaa
    47281 atagccgcgt tggttttcat caaatcggca aggtacaaat cgcaaaattt ggccttcttg
    47341 cactaaatat ttgtctaagc cattataata agaaccatca acagtattag aaaaacaaat
    47401 tggtctgctc caatcgcctg cagctaccat tgccaatagt gctaagtcga attttattat
    47461 tctggttttg cctaaatccc aagttatttc atcgaccact ttatctttaa attgagcagg
    47521 aacgacttga tatttttcaa ttgcagcttt atctactttc aattttaatt ttgtggtagg
    47581 caaataattc acggcttcac ctctttgcgt ttgtgcttta aactgttcat catcacttaa
    47641 aacaaaagcc atgatgtttt gaatgtcata atattgagat ggatctgcaa atccagactg
    47701 gtcattaaac tggataacat ctctggtgtt tcctctatat tttgcatctg taaaatcttt
    47761 gtagaaagga attgaacctg atttgtttgc tgcacgatgc aagaaattga tataccaatc
    47821 aacacctaat aagcttaagt tgacgatgcg tacatctgta cgaattcctt ctacttcttg
    47881 agcataccat aatggatatg tatcattatc accttgtgta aataatattg cattgggcgg
    47941 acaactttct agatagttgc ttgcaaaatc gcgagccata tatctaccat gtcgattatg
    48001 gtcgtcccag cctgctttgc ccataatata aggcacaaac aaacaaagaa tagtaaccgc
    48061 tacagctaca ggtaatgctg gtagttttcg ttttaaataa tcatagattg ccgctacagc
    48121 caagcctatc caaatacaga aggtaaagaa tgagccgaca gtaccataat cacgctcgcg
    48181 tggctcgcgt ggtggctgat tcatatatac tatcaatgct atacctgtta ctaaaaacaa
    48241 caaaccaaaa atcatggtgt attgcttatt gcgttgatac ataaaataaa tccctattaa
    48301 tcctaaaatc agaggcaaca tatagaattt atttcttgcc ttttcgttta atatctggaa
    48361 tggaagtcca tcttgtgaac ctaaatggat attatcaata aatggaatac cgctgagcca
    48421 gtcgccattc attctttcat ttccaggtgt tccttgtaaa tcatcttgtc ggccagagaa
    48481 attccatagg aaataccgcc aatacatata tcgcaattga taatggaaaa aatatttcaa
    48541 gttatctgca aatgtaggtt tcccttgaag gccagtccaa tattgatata atgcttctgc
    48601 gctttcacca tcatttgttg gtcccatgcg tggaaacaac atcatatctt ctggattgta
    48661 ggtatattct attttttctc cttttgcttc atattttttt gttttaaggt caggataata
    48721 atccttgcct ttagattcat atcctgttgg ctgagcatcg tataatggac cactaacaag
    48781 aggtcgagaa ccgtattgct cgcgatttaa atatgacaat aagctaaatg catctttagg
    48841 tgcattcata ttgattggcg gattagcaac agaacgaatg ggcaccattg catacgacat
    48901 gaaaccaata aaaataacta agaaacaaat tgaaagtaat tgtaaatctg cgtgtttaat
    48961 tttattagca taataaatta gtgcaatcag tatggcaaat aataaaatcc atgccacaac
    49021 gaatcctata ttgaatggta aatgtaaaat atttacggag aagaactcta tcgaaccagc
    49081 aagctttaat gcacttaaaa caacacctat ctgcaccgtt cctaaaagca caaaacccat
    49141 aaatgtggca ataataagtc ctaaaatagt aggtttgaat ttcttgaaat aatacaccaa
    49201 tacagcgcat ggaattacta ataatgacaa taaatgcacg cctaatgata cacctatcat
    49261 atatgctgca aataccaacc atctattgcc atgtggttca tcagcttctt cattccattt
    49321 cataaccgcc cacaatacaa tagatataaa gaacatggaa gatgcgtaca cctcaccttc
    49381 tactgcggaa aaccacatcg tatcagagaa tgaacatgct aaggcgccta cagatgctga
    49441 accaacaatg gcaataatat ctgcaatcga aaactcttct cctgctttag tgattaattt
    49501 ctttgccacc attgcaataa caaagtataa gaacaacaca catgcagcag acatcaacgc
    49561 tgataggaaa ttgaccatta ttactggatt cgatgggttg aataaagtaa aaattctacc
    49621 taatatcatg aatagtggtg cgcctggcgg atgcgctact tgcagtttat acacacagga
    49681 aacaaattca ccacaatccc acaagctacc cgcagattcc atggttgaag tgtattgcca
    49741 aagagaaatt gcaaaaacga taaagccgac aactgcattt atcaatttgt attgtttgtt
    49801 cattcaacag attttggaca aaaatataaa aaaatagccg ttcattggca tttattgatt
    49861 gtgaataaat gtaaatgtta ataaagtaat ggcggtgaaa tattttatat ttcttaaaaa
    49921 caaacttatc ttatgaacct atgaacaaaa taatttagca aaacaagtaa acctatgtaa
    49981 ataaatgcca tttttactaa aactattgac atctttttag gctataaatt attttactat
    50041 cttagaaatt ttatgtttgc atttagatat atcaactatg agtaaaaaaa gtaaaaataa
    50101 agattatccg ctagccacct attttggtta ttacaatcaa tttttagata caaaaattgg
    50161 caaagcatta aatctcagaa aaatatctga gcggtttgtg tatgaaggca ttaaaaatgg
    50221 cgtaaaagtt gccagcaaca ccacacgtgt ttttaaaaac ttgactggtg gtaatgcaga
    50281 acgattaaca cccaaaacag caacagattt atttgattta aactatacag acgagcaaca
    50341 aattgtacaa gaatctgtgc aacaatttgc tgtcaaaatg cgtgctgcgg ctgaaaaagc
    50401 agacgacacc gccagcattc cagacgaact ttggcaagaa ttcaacgatt tacaattagc
    50461 ttatatgcaa gtgccagaga atctaggtgg catgatgaca caaaaatcta cggtgacaca
    50521 aatgattatt gccgaaaacc ttgcacatgg cgattttgga caagcactcg ctttttatac
    50581 caaacatagt gttctcaatg caattattca atggggaaca gccagccagc aagctgcgtt
    50641 agtgccagcc tttttaggag acaatccgga aactgctact attgccatca acgaagcaag
    50701 accattgttc tcaccatttg acctaaaaac aaaagcgaaa aaagaagaca agcaatattg
    50761 tatcaatgga agaaaaaaca tggtaccatt agcagaaagt gctggatata tattagtggc
    50821 ggcagacaca gagcaacaag gcattcgtct ttttatagta gatagcaatg ctgaaggtgt
    50881 aactataact gctgatagaa gtatgggcct aaatgtagca gctttatgtg caatcgaatt
    50941 tgatgatgta atggtcgatg aatcagcaat actcggcgat tccgatttta attatgtcga
    51001 atttattgct tatagtaaac ttgggtggtg cgcaatggct gtcggatgtt gccaagccat
    51061 tttagattat gtaattccat acgcaaatga cagacatgca ttcggcgagc ctatcagcca
    51121 tcgtcaagcc gttgctttca tgattgctga tataaaaata gaactagatg gcatgcgtat
    51181 gctaacacaa agagcaacag cacttgcaga gcaaggtgtc gcttttgaac gagaagctta
    51241 tttagcacat gttttctgta gcgataaagc tatgcagata gctagcaatg gcgtgcaatt
    51301 attaggaggc cacggtttta ttcgcgattt cccagtagag cgttggtatc gcgatattag
    51361 agctgttgca attggtatta atggtattca tctataaaaa aataaaacaa tgaatttaga
    51421 agttccaagt aagtttaaag gaatacaaaa ctttgctatc atggctgcaa agacaatatt
    51481 tagacctgta tctagaaaat acgatgtgga agaacatgca tatcctgtag aattggattt
    51541 atttgctgca ggattaaaag gattaaatga aggtggcgat gcgccagttg gtggtgctaa
    51601 tacaaaaaaa gaaaatgtcg tagctggcga agtgagaaat ggcgccaata tggcaaattg
    51661 tgtcaatatt caagcgatgt gttatggtga tgtcggttta ttgttatcga taccagggca
    51721 aggcttaggc aatgctgcta taagtgccgt tgccaataca gaacagctaa aacgctttgc
    51781 taatacttgg gaagccatgg caattacaga gccaaattgt ggttctgact cttcagctat
    51841 cacaactact gcacgcaaag aaggtgatta ttatatctta aatggcgaga aaatatttgt
    51901 tacagcaggc gagcgctgcg actctgtagt cgtgtgggca accttagata aatcgatagg
    51961 aaaagcagcc atcaaatcat tcgtggtgga aaaaggaaca cctggaatgg atttggcacg
    52021 tctcgaaaaa aaattaggca tacgagcatc agatacggct gctatcactt ttacagattg
    52081 taaagttcct gctgagaatt tattaggctc accagatatc gatacaaaaa aaggctttgg
    52141 tggtgtcatg gaaacatttg acaatactcg tccttttgtg gcatctatgg ctaatggcgt
    52201 tgctgaagct gcaatagagc gtacaaaaga attattagat gatacaatta cattggatta
    52261 taaaacacca atcaacaatg taaaggctgt ggaagctgaa ttgtatagaa tggaagctga
    52321 ttatgaagcg gcacgtttgt tgacacttag agcggcttgg atggctgaca atagaaaacc
    52381 aaattcatta gaggcttcta tttgcaaagc taaagcagga cgagtgacga atcaaatagc
    52441 attaaaatgt atcgagttgt gcagctcttt aggttactct tatcaagaat taattgaaaa
    52501 atgggcaaga gattctaaaa ttttagatat ttttgaaggt acacagcaga ttcaacaatt
    52561 aatcatcgca agaagattat tgaacaaatc atctgctgag ctaaaataat tatgagccaa
    52621 cattcctcaa gaaaaaatac tgttcaattt tttgaattag caacaaaaat tccaggatta
    52681 gcaaaggatt taccaaaaat tctaactgct ttatattaca attacacagt aaatcctacg
    52741 tcagatatta ctattggtca gctatttcaa aaatctgtat taaaatatcc agaacggcca
    52801 tgtgttattt accatgacca agaatggact tatcgtgaat ttaatttgtg gattaatcga
    52861 ttagcaaatc actttattaa aattggcatt aagaaaggtg atgttgttgc cgttttatta
    52921 gaaaacaggc cagaaattct tgctatttca ttagccttag caaagattgg tggcattaca
    52981 gcgctactca atacatcaca aagaaatcaa acacttttac atagtattca acttgccaag
    53041 cccaaacttt tattaatagg ttctgaatta acggcacatt atttagaaat aaaagaggaa
    53101 ttaaacaata atacttgcca aactttttta atagaacatt taagggtaag cacgccaaaa
    53161 ataaaagagc ttagtgtctt cgatactaac agtaaaaaat ttgcaggaac agagccaatc
    53221 attcattatc atatcaatgc caaagatgcc gttatgtata tttatacatc aggtactacc
    53281 ggtttgccaa aggcatcaat cattagccat ttaagatgga taaaaggata tagtgcattt
    53341 ggacttacat cgtttcgact cacgccagaa gatagattat acgttccgct gccattttat
    53401 catgcaacag caatgatagt ttgttggtca tcagtgattg caggtggcgc atcaattatt
    53461 ttaaaaaata aatttagtgt aaaagatttt tggaatgata tagattatta ccaagccaca
    53521 ggatttggat atgtcggcga aatgtgtgtc tatttattaa atacagaaat tcatccaaaa
    53581 gagaaaaaca atacgcttgt caagatgatt ggcaacggac ttcgacctga aatttggaag
    53641 aaattcaaag atagattttc tgtagagcaa gtttgcgaat tttatgcatc aagtgaagga
    53701 aatattgcct ttttcaacgt ctttaatatt gacgaaacca tgggattttc cattacgaat
    53761 tttgcgatag ttgcttatga tcaagaaaaa gaagataccg taaaagataa aaaaggttac
    53821 ctacaaaaag taaatactaa cgaggttggt ttgctgcttg gcgaaatctc agaccgttat
    53881 ccatttgatg gttatacaga aaaagagaaa tcagaaagta aggtacttcg aaatgtattt
    53941 aaaaacggcg atgcatggtt caatactggc gatttagtca gagacatggg ttacttgcat
    54001 acacagttcg tcgatagact tggcgatact tttagatgga aaggcgaaaa tgtatccacc
    54061 atagaagttg aaggtattat taatcaatat gaaaatattg aagagtgcat ggtgtatggt
    54121 gttgaaattc ccaattgtaa tggaagagct ggcatggtca atattattct aaaagatcaa
    54181 aaacaaaaat tcaatttcga ggcattctat gcttttattc gcaaggaatt accaccttat
    54241 gcaattccta tttttataag attttcttct gcaatcgaga ctacaacaac atttaaacat
    54301 caaaagataa aattaaaaca agaaggtttt gattacacgc taataggaaa taaagtatat
    54361 gcttttatcg ataaaaaata tacattaatc acaaaaaaaa tattgcaaga catcaataat
    54421 ggcgtctata gattctagta ttttataaaa tggaaataaa aaagtattga ttaacacact
    54481 caaacacaag gaattcggct tcaatttatc ttggtaaaat tttattaata tttttgagga
    54541 agaatattct tgtttttttg aaaaaagtac ttatttttgc gttccgaaac tgaccgatgg
    54601 tgtaacggta gcacagaagt ttttggtgct tttagttgag gttcgaatcc ttgtcggtca
    54661 acaagtcctg aattttttca ggactttttt tttataaaaa acaagattta tttcttctta
    54721 aatacataca tcaaactgga ataatttcca ctttttgatg cagcaagatt ggcaaaaaag
    54781 ccgtttttaa gtgctttcgc tattgccata aagcgatttg atttcaaata ccattcgcta
    54841 tgcaaggcta tataataagc atcaaaagtt aatggatatg tcgtaagcaa ttcaaatcct
    54901 aagcgttcca ttagaacaac aatcgtagct gtattaaaat gatacaaatg tcgaggcaca
    54961 tcataagccg accaatattt gccataatat ttcttgtctt ctgcatcata atttg
//
LOCUS       DAOQQV010000004        52543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_176_length_52543_cov_8.572645, whole genome shotgun sequence.
ACCESSION   DAOQQV010000004 DAOQQV010000000
VERSION     DAOQQV010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 52543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52543
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176_length_52543_cov_8.572645"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLL59_00900"
     CDS             <1..690
                     /locus_tag="PLL59_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMY22385.1"
                     /translation="RELYANIPESKIRGYKNGRFSFNVIGGRCEECQGGGMKLIEMNF
                     LPDVYVQCPKCLGRRYNRETLEIKYKGKSIYDILEMSIEEAVHFFEHIPSIYNKIKTL
                     NDVGLGYVRLGQAATTLSGGEAQRVKLSEELSKRDTGKTFYILDEPTTGLHFEDIHVL
                     LNVLQKLVDKGNTIVVIEHNLDVIKVADHIVDLGPEGGRGGGEIIATGTPEMIVKNKK
                     SITGQFLKDEL"
     gene            771..1208
                     /locus_tag="PLL59_00905"
     CDS             771..1208
                     /locus_tag="PLL59_00905"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HMY22386.1"
                     /translation="MKLLSFYLLVFNFQLHNASETPNVIGIWTCSDKDCKVEIYKNGN
                     SYEAKLIWARKEIDEKGKPKLDIKNPDPTKRNLPIIGSKTLWNAQYNPATGYFENATA
                     YRNGRYFCGKFKLNNNGSLTIIGYHCNFKFLKFSDTWTRVNTH"
     gene            1342..2211
                     /locus_tag="PLL59_00910"
     CDS             1342..2211
                     /locus_tag="PLL59_00910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMY22387.1"
                     /translation="MTASNTAHNLKVRNVHFNFDKVPKHWILGSSVATHFINSMHIVF
                     PEGEKFFVRSVRHFAKDIKQEELRTEINSFCGQEGVHAREHQRFWEVMEEQNLKPKNF
                     ANFLKTTMFAGKYSYEKISTKLLNTISPQLGDKFQLSVTTALEHYTAIIANALFLEPL
                     ATNKNIAPQMLELLHWHASEEIEHKAVCFDVLKEVDDSYLLRVSGMGVASILLWSSIA
                     TGQVYFIIMDKDKSIIKMPFEIFILFNAIVFGEVGKNLSKHFLDYFKPNFHPNDIDDR
                     YLIEKFFKDKTYA"
     gene            complement(2208..2615)
                     /locus_tag="PLL59_00915"
     CDS             complement(2208..2615)
                     /locus_tag="PLL59_00915"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HMY22388.1"
                     /translation="MTNRKPIKSVFFLRCPSCGIGSLFKSKHSYELNKILDMHEHCEH
                     CHEDFEVETGFYQGAMYMSYIITCALCLAILPIYYLFNNTREAFLDNAMYYLIACFAI
                     LIFTAPYVIQLSRAVWLKMHVKFFKSKTEENHH"
     gene            complement(2631..3251)
                     /locus_tag="PLL59_00920"
     CDS             complement(2631..3251)
                     /locus_tag="PLL59_00920"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMY22389.1"
                     /translation="MSDLKCSKDKAPSHLDATFWDNLWQNKITGWDLNAVSPPLQLYF
                     DSMTCKNKSILIPGCGNAYEAIYLLENGFKNVTVIDIAPTLVQQLLSKLAKYAGKELT
                     IICGDFFKHTGQYDFIIEQTFFCALDRNLRTAYAIKMKELLKETGKLAGLLFDKEFKD
                     GPPFGGSKTEYEQLFNEHFNIQQMDVCMSSVKPRLGAELFFELTKK"
     gene            complement(3248..4051)
                     /locus_tag="PLL59_00925"
     CDS             complement(3248..4051)
                     /locus_tag="PLL59_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMY22390.1"
                     /translation="MEILGYIAALFIGLSLGLIGSGGTILALPILVYLFKINDMQTAT
                     HYSMFVVGITALIGAFKKYNEKLVDIKTAIYFLIPSLISLFLTRKVLLPYFPNNFFNI
                     GNVQITKDIFLLLLFAIVMLISSVSMIKNAQQEDVDCFAKIPNANANHTKTTLVAMLI
                     GMLTALLGAGGGFLIIPTLVLLKEHCMKRAIGTSLVIITVNSLIGFFGDLQLSVDWKL
                     VLSFSSIAVLGMLLGIKLSDKIAGATLKKAFGILVLIMGFFIIIKELVL"
     gene            4111..5196
                     /locus_tag="PLL59_00930"
     CDS             4111..5196
                     /locus_tag="PLL59_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HMY22391.1"
                     /translation="MLQNKTVYGIGLMSGSSLDGLDICYVKFEQQKSSICYTILQATC
                     IAYDKQQTEKLRKAAQLSAFEFAALHTEMGKFFGSLTHQFIQQHQIEKLDFIASHGQT
                     IFHQVATGFTTQIGCGAQIAAQTGHKVVCDLRTADVAYGGQGAPIVPIAEKYLFPDFS
                     FFLNIGGIANIAIHNKEKTTIHAYDICAGNTMLNYLAKQKNLDFDDNGNLARNGKVIN
                     ELLEKLNAIPFCKQAAPKSLGTEHIYETWIPLFDECDSTIEDKLATAVEHIAMQVGKE
                     INNLIPHHNSSLFITGGGALNTYLIERIQQHTTYKTIVPDMLTVQYKEALAMAFFGLL
                     RIIEQPNCLASVTGAQKNVIGGAVYLP"
     gene            5254..6231
                     /locus_tag="PLL59_00935"
     CDS             5254..6231
                     /locus_tag="PLL59_00935"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMY22392.1"
                     /translation="MTGASGFLGGELVKQLVAQGESVRIIKRASSSLTHLQSIIHQLD
                     IVEADILDIPSLEYACEGVEKIYHCAAVIGYDSSYYNAMYKCNIEGTANVVNVALSKN
                     IQKLIHVSSIAAIGGKPNELITEETKWEKNEWTTHYGITKMLAEREIWRGIQEGLVAA
                     IINPGIIVGIGHEHKSTMRIFKNIQQQKMPFYTNGINGFIAVEDVANLCVQLMNSDVQ
                     AERFIAIEGNYSFKTYFDSIATALQVSPPKKALNKTLGNFFLLLDYLASLFTNRKRGL
                     TKENMTVSMEAFTYSNEKIKQQFNYTFQPLQEKINQIAQQLTSYERSNS"
     gene            6212..7246
                     /locus_tag="PLL59_00940"
     CDS             6212..7246
                     /locus_tag="PLL59_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HMY22393.1"
                     /translation="MNEATRRKILGVWFIIGVGMLLGQIILGGITRLTGSGLSITEWK
                     AILGFLPPMNEQEWQLAFIKYQQFGQFKLLNTTMTLSQFKSIYFWEWFHRLWARLIAL
                     AAIIPLVYFIIKKIALKEDIIKILIAILLGGVAGAVGWIMVQSGLKQDAVLVNPVNLM
                     SHIVVATIIVAYVLRVGLEYLYPKQILPFDNNKRKLFSILIILVFIQIALGALVAGSK
                     AALVCTSFPLMNGNYFPTLFGNHETDVQLILQFIHRNVAYFIFFYVLFVFVRTKKVDA
                     QFQFHRTRYLLLAMVLIQVTLGVLTLLYSQGKVPVTLGVLHQFGAFLLLIFSIEGHYF
                     IKYRSAKNER"
     gene            7288..7854
                     /locus_tag="PLL59_00945"
     CDS             7288..7854
                     /locus_tag="PLL59_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22394.1"
                     /translation="MQLKVSSITNLTDARFFSAIGAHYLGFCFDVLNVNNIHPKKANE
                     IIAWLHEPVVVAEFGAHQTKEEIEYIASQIDISEIQIPISNNDIGNLHFDKFIEIELT
                     DIEKTLPASDYYVLKTEQINTEDLALKEFIAHHKVFIDTAFTKENIEEIVAKLQPYGI
                     QITCKREEKTGLSSVDEYAEITAAIGFS"
     gene            complement(7864..8511)
                     /gene="trmB"
                     /locus_tag="PLL59_00950"
     CDS             complement(7864..8511)
                     /gene="trmB"
                     /locus_tag="PLL59_00950"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HMY22395.1"
                     /translation="MASKSKLQKFAELADNPIVFQKNPILKGKWHADYFKNNNPIVLE
                     LACGKGDYTLGMAKLFPEKNFIGVDIKGNRIWSAARIANLEGLQHVAFIREQIDLLYN
                     YFEEAEVDEIWITFSDPFLRDGDAKKRLTSTKFLPIYKQILKPGGLIHLKTDSDVLYA
                     FTKETLAEFPSSIVKDYSDVYAMQKNDELHGIQTYYEKMHLKDKRTIKYLCFQLQ"
     gene            complement(8558..8797)
                     /locus_tag="PLL59_00955"
     CDS             complement(8558..8797)
                     /locus_tag="PLL59_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22396.1"
                     /translation="MQQNSTLDLLIKHLYQETDTDENAIVQQLLASDTKLQEQYQQLC
                     ATKNAIDEHGGDGPNDAIIQQILSYSQQKEHSITY"
     gene            complement(8890..9423)
                     /locus_tag="PLL59_00960"
     CDS             complement(8890..9423)
                     /locus_tag="PLL59_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22397.1"
                     /translation="MIAYSIGTKIEHPTFGKGIIIDDSDEYVYNLYFPEHGEKEISKR
                     FEGFVLIKMIEKDTNSVSLEDIKSVLSDIIAPLTEPIEDCKIAEKWEGGNLILQPKDK
                     SLQAKEIPIEGFFHKIVMTRDRLRVMEQKINASKLSDAEKVELQQYITRIYGSLTTFN
                     VLFRFKDDYFKGTGGTD"
     gene            9559..10935
                     /locus_tag="PLL59_00965"
     CDS             9559..10935
                     /locus_tag="PLL59_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY22398.1"
                     /translation="MKRYLFLSIGFIFCFNLQSFAQWGSIGDRIVDGLSNKAQQKIES
                     EISNGADRAYEKSKSSVKESVKNSGSKSKNSKSSSSNSNDNNNEDNDGQSNSSASSSK
                     NTSSSSSLKAYGKFDFIPGEKVIAEENFSQDAIGDFPAKWNTNGTGELVTIDGQQGKW
                     LKFGPESIIYPEFVTNLPENFTVEFMLASSQPFSYYSTAFNFFFAPLANPAKNYVNWK
                     RFGGDKKNGVLVALQPEAAGGNAMGQKWINTFDETGDEIIKNQTDFPEFNHENKNVVK
                     ISIWRQKQRLRVYVNETKIWDIPKAFSATAKYNFVGFRTDGYHNDNDAYYLSNLRVAI
                     GAPDTRHKFLEIGKYSTTGIKFDVNSDKIKGESYGTLKDFAAVMTENPDVKVKIVGHT
                     DSDGDDASNMTLSKKRADAVKAALNKEFGIDNSRMETEGKGESQPAEPNTTAEGKANN
                     RRVEFIKL"
     gene            10947..11738
                     /locus_tag="PLL59_00970"
     CDS             10947..11738
                     /locus_tag="PLL59_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22399.1"
                     /translation="MKKSTLILAVLISIFSVSCKKENLGIDYSKIRVKQIATNNNGSS
                     MDTIRFFYTNDNVKTVTNYSANPTFLYTFNYIKNGNKYDVSSIYGTVNTHEGFYNINS
                     QGFIDSSRFTNIITSAFNNRDKYYYNSDGYNTRSITNYNTYENDVTKYYTVEGDFRYW
                     IYDFKHFTTPASSYIDSIAFDYYNDENHVAFEYSLQDKFGKLNKHLVKKRYNYDRTTG
                     ILKRTIEYQYELDSKGLVTRRISSYYNQPGNILSSTVDSRYTYYE"
     gene            11767..12564
                     /locus_tag="PLL59_00975"
     CDS             11767..12564
                     /locus_tag="PLL59_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22400.1"
                     /translation="MKKTTLILAVFISIFSVSCKKDKLDSDCNIASITINDNPSFKST
                     FKYDNQNRISEYTEEGGAVVYKFEYTGLSGKLKLFLADTLYETKNLTFDSKGRLTSFE
                     DYLPFGGDLLRYFYLFTYNSDGYLASTIQTLTGGSYDSYYRDSLIYSGGNLIKNFRKH
                     RNNTIQTTTEFSYGSNTNKHWNFYWNAFTEPFSILSGYTFLYPLLGKTSTNLPTSVVV
                     TEGSFIDNFSYAYFINSAGNATEYNEMRSNTVTGNTSSNYKLTYTCD"
     gene            12624..13124
                     /locus_tag="PLL59_00980"
     CDS             12624..13124
                     /locus_tag="PLL59_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22401.1"
                     /translation="MKKISTNICIVLSIALFFVACKKDDNGKVRKQHISLMADGVAFS
                     VTTDGVDVEEADAHIRDGNKFQLDATTSTKDFSINNKNVPSTGNYVMNGAGTVFDFAQ
                     WEDGPALFSSLGSPKSHFTFNISNIAGSPSDYVRYVEGTFSGVLYNNTQTDSVIITNG
                     QIHIID"
     gene            complement(13176..13949)
                     /locus_tag="PLL59_00985"
     CDS             complement(13176..13949)
                     /locus_tag="PLL59_00985"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMY22402.1"
                     /translation="MEINVNNTFFTGKFLLHLPSVDSTNNFAKNHIANNSPIDGTVIF
                     ADEQFAGRGQSGNKWQTEAHKNLTFSIIYQTSFLPATAQFLLNKAISLGVWAMLDAYF
                     RLEKNKPIVEVKWPNDIFVNNKKIAGILIENTIAGMNLKHSVIGIGLNVNQCVFADGV
                     NATSMQLVLEKSLDRNQILNDILQHIEKYYLLLKQQRNEVLDKEYLANLYQKEKWTFY
                     KKGEEIFEGKIIGIDQFGKLILETMTGKESFAFKEIQFL"
     gene            13997..14377
                     /gene="rsfS"
                     /locus_tag="PLL59_00990"
     CDS             13997..14377
                     /gene="rsfS"
                     /locus_tag="PLL59_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HMY22403.1"
                     /translation="MEKVKTKKKAKTISADALNELVVSLIQDKKGNNIVSLDLRHIPE
                     AITDFFVICHALSTTQVRAILDHLEEEVKNQTSIKPYHLEGRANGEWCLIDFGDVVAH
                     IFQEEKRDFYQLEDLWSDAIIKHY"
     gene            14475..16421
                     /gene="ftsH"
                     /locus_tag="PLL59_00995"
     CDS             14475..16421
                     /gene="ftsH"
                     /locus_tag="PLL59_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HMY22404.1"
                     /translation="MDFLPENEEPKKKKGGFNSYWLFAILIVGLLAAQFFSLNFQIKQ
                     ISEDEFFRKLVPSGDVAKITIVNKELVEVFIKEDSLKAKKQYADIKLNKLKSINKGPH
                     YSFEITSIEAFKQKLDAIQANTPYESLIKVNNEIRKGIFDIIGWLLPFILIIGIWMFL
                     MRKASGGMGGAGGQIFNIGKSKANLYDKDMKVNVTFDDVAGLDEAKEEVKEVVDFLKN
                     PKKYTALGAKIPKGVLLVGPPGTGKTLLAKAMAGEAQVPFFSISGSDFVEMFVGVGAS
                     RVRDLFKQAREKAPCIIFIDEIDAVGRARGKNMMQSNDERESTLNQLLVEMDGFSGDK
                     GIIMLAATNRPDVLDSALLRPGRFDRQISIDKPDQKGREQIFKVHLKKLTKLGPDVTP
                     EKLSSLTPGFAGAEIANVCNEAALVAARKEKTQVEVEDFNEAIDRSIGGLEKKNKIIS
                     PQEKEIIAYHEAGHAICGWYLEHAHPLLKVTIIPRGIAALGYAQYLPKEQYLYRTEQL
                     MDEICMTLGGRAAEEIVFGKISTGAQNDLQRITQLAYAMVTVYGMNDKVGNVSFYDPQ
                     QDTTFTKPYSDETAKVIDEEVRKLIDTAYQRTLNLLTEKREQLNTIAKSLLEKEILFK
                     SDLEELIGKRSFDEEPLPVAISID"
     gene            16487..17305
                     /locus_tag="PLL59_01000"
     CDS             16487..17305
                     /locus_tag="PLL59_01000"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HMY22405.1"
                     /translation="MSNKTILKIYSIALLTTGMMFLVFLYLNQSFDGHFWQSMQQSKS
                     ALCAEYCELDQPTHFFRQTINSYSNLMYFFLGVIVIAVAIYDLTDKRANKNPLQRFPA
                     LSFLFGICLLYLCFGSTFFHASLTWLGQRVDMNATYSICLTLIGISSYRYYIKQTENI
                     ANKAVIIIGLISLIFLFIPLHLLVSSSILLPLLISIAVFFVAKNYTNNKEKYKLHFAI
                     LSLLLVIGAFILRTLDVKKIVCNPTSIYQAHALWHVFTGMSAFLLYWFYRSEQA"
     gene            complement(17306..18445)
                     /gene="bshA"
                     /locus_tag="PLL59_01005"
     CDS             complement(17306..18445)
                     /gene="bshA"
                     /locus_tag="PLL59_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HMY22406.1"
                     /translation="MNIGIVCYPTFGGSGVVATELGKGLAMKGHNIHFITYKEPARLL
                     DTFYENIFYHEVRLNDYPLFDYAPYETALASKLVDVAINAKLDIFHVHYAIPHASAAY
                     MARQILLQKGMNVPVITTLHGTDITLVGRDATYEPVVTYSINQSCGVTAVSESLRQDT
                     FSHFDIKNEIEVIPNFIDFSRFKKTNKEHFKKAIAPNGEKIIVHTSNFRKVKRVEDVI
                     HVFCKVSKQIPSKLLMIGDGPERQYAETLARNLCTIMDVRFLGKQEAVEELLAIADVF
                     LLPSESESFGLAALEAMACEVPVVSSNAGGIPEVNIDGETGFLLNVGDIQGMADKTLS
                     LLQDESMLLQFKKNALAQAQKFDISVILPQYEKFYQRIKEGGNCK"
     gene            complement(18593..20611)
                     /locus_tag="PLL59_01010"
     CDS             complement(18593..20611)
                     /locus_tag="PLL59_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HMY22407.1"
                     /translation="MIHSNWSIPSFLQDAMQTLALKKIWIPLLLLLAPLLSNAAFEQH
                     LTWTHAVEKIGENEYKVKFICTLEEDWHTYSQFTKEGGPLPTQFRFAKNNDIELIGKV
                     EEIGKKKTAFDELFGVDVTTFLGDVTYVQKVKLKHSKAVLKGEFDGMVCKDEFGCMPY
                     GPEKFAISFDGTAATTVAVKKQDTLSQAKTGDTTATAVVSTVPASTKFDFSTAKNACS
                     ADAQTTTKSWLWIFIAGFIGGLLALLTPCVFPMVPLTVSFFTKGGKEQKTGISKALVY
                     GFSIIIIYVSLGLIITSVFGSDALNAMSTNVWFNLLFFVVFIIFALSFFGLFEITLPS
                     SWANKTDSLSGKGGYLGVFFMAFTLALVSFSCTGPIIGTLLVMGGSSLFFGLLGFGIA
                     LALPFTLFALFPQWLHSLPKSGGWLTTVKVILGFIELALAFKFLSTADMTMNWNILRF
                     EPFMIIWILIFVIMAFYCLGIFFKTKHNTIVKSLGVATLLFAFYLIYALINYQPVGLL
                     SGIIPPTSYNFKTKNETKFVHFKDYDEGIAYAKKHDMPVLLDFTGFGCVNCRKIEDNV
                     WTDEQVIERLKEYVIISLYVDDRKALPENEWFVSTATGKEREVKTVGQKWSAFQAEHF
                     KTNSQPLYVIINTKEQLLNQPVAYDFSKDKGNYISFLECGLKMNKQLK"
     gene            complement(20618..21319)
                     /gene="purQ"
                     /locus_tag="PLL59_01015"
     CDS             complement(20618..21319)
                     /gene="purQ"
                     /locus_tag="PLL59_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HMY22408.1"
                     /translation="MKFGVVIFPGSNCDADVIHVCSKVLQCETRILWHKDTHLQDLST
                     EDCIMIPGGFSYGDYLRCGAISRFSPIMEKVIEHANNGGYVFGICNGFQVLCESHLLP
                     GALLMNNHQKFVCQNQYLLPQTKDSAITRHLDLQQALNIPIAHGEGRYYADEQTLQSL
                     NQNNQILFRYSDASGNINEAANPNGSIENIAGICNAGRNVFGMMPHPERAAEEVLGNT
                     DGKKLFESLLNLVNT"
     gene            complement(21379..22350)
                     /locus_tag="PLL59_01020"
     CDS             complement(21379..22350)
                     /locus_tag="PLL59_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22409.1"
                     /translation="MLSFFKSQQPPAILAFFALFILLKIPFFIIGVVPVFPVTNLWNS
                     CDIIFPMISWWNVLLAQLCLFVQAIWFNYLFHKSDYHESSTMLPALYFTLLSSCFSAF
                     NVFSIYTIVVFILLMLHQTFITITVKESTRLECFNVGALGGLLCLIDVHFIVFIPFLF
                     LILLVLKPFRFNEYLMLIFGILFPVYMALSYSYLTDTFLNIANFNFSILPQIILPKSK
                     IDIAMLIIVILYLFFSLISIRDIFYSIGFRRRKNLYMLFVSFIGFLFVIISNKLNDLA
                     AISVLLIPMSCLLALLMLRIRKKRIGEILNAIFVIVIIVTNIIRAVN"
     gene            complement(22374..23900)
                     /locus_tag="PLL59_01025"
     CDS             complement(22374..23900)
                     /locus_tag="PLL59_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22410.1"
                     /translation="MKRYSSEIKDNKTISNPRTTLLSCFAFILFCCFNHQTTAQTIIK
                     SSAEKVSNRFSDYEILGKNDIGLLVHYFGQNESELVAYDGKLKVSNRRELPFKGKAAT
                     VESFILLKNKILVFYTTNSENYQYLKLKVLDEKLSIPVESILLDSIPLISIGKGKAFY
                     VKTSPDKSKILTFNMLKTKAAYFVRFTILNDSLQILNRNIFTNTDGGNIALKSIKIND
                     NGNVIAVTGDENNSNDDYAYNKYTMFLFNRTTNTIAEQVMQQSDHVFKNLITEVSSKR
                     DVVYVVAAYKSTANKSDIGMFYQIIDFRTNTVLLNSKLPFSDAMLAKSQTKEFKTWTD
                     KAVLIKPKRIIPRSDGGFVVITEGENKITRTERIANNNYGYYNATPYMPAVRYIDQNF
                     FYNISLFSINTDGTLDWQTDMPKAQVSENDDGYYSSFAFFEANNALKFLYNEDFYTIG
                     NFVEYSINANGLSKRNSVMNSEKQDLVMVPLKAKQLDANSIVIPSEQKRNLQFVLFQY
                     "
     gene            complement(23907..25142)
                     /locus_tag="PLL59_01030"
     CDS             complement(23907..25142)
                     /locus_tag="PLL59_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMY22411.1"
                     /translation="MNTNQEQKQDYWSYVKRQFKKNKRALFSFYVVLFMLVIAIFADF
                     LANEKPIVCTYQGKTYFPILKEYAVKLKLGKFPPELNNIIWSETKFDFAIRPPIPYSP
                     TTQDILNSGFVSPYGKQDVPSIRWRHWLGTEGIGRDILSALIHGTRIAFIIGLISMSI
                     ASVIGILLGSLAGFFGDKGIQISRAALLMGILCSMLGLFYANAVGGLFTGIAVFFACI
                     LIGYLVSRVLKKIPFLAPKTSLPIDIIVQRMIEVLVSIPRLFLIIAVVAISKPSIFLV
                     MVVIGLTSWTEIARFIRAELLKIRSLEYIEASEALGYPKWRILLKHAIPNALSPVLIT
                     IAFGIAAAILTESFLSFIGLGIPPETLTWGKLLSLAQGGRSQLWVAIFPGFAIFVTVT
                     LFNLIGEGLTDALDPRQKK"
     gene            complement(25149..26567)
                     /locus_tag="PLL59_01035"
     CDS             complement(25149..26567)
                     /locus_tag="PLL59_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMY22412.1"
                     /translation="MLKYIFKRILLFIPTLFMISLLTFFLSTNVPGDPVEQMLNSNSE
                     MGSSAKAQAAEKAYIDKRKQLGLDLPTFYFSITNSATPKDLYTIPKKFHRENLSKLIE
                     QYGNWKQINAYYLSLKKLEESLGNVTSDSVNADALIAMKSDVQQLYIEPEDKTINLLF
                     KDMLAQLHVADNLKVLETDIVNSSKLYADVTQHATTYKKFIPAIQWYGTKNQYHQWIT
                     KFMVGDFGISYQDEKPVKTVIWEAIRWTVLLSIISILLTYLIAIPLGIVSAANKGGIK
                     DQTISTTLFILYSLPSFWVATLLIMFFGGGDFFNFFPSFGVPQATENMSFFTKFTNYV
                     WHLTLPIICYTYGGLAFISRQMRGAMVNSLSQDYVRTARAKGLDEEKVLWKHAIKNSL
                     LPIITLFANVFPLAISGAVALELIFSIPGMGKTAYEALVARNYPVVYTIVMFSAILTL
                     VGYLVADILYAVVDPRISYNKK"
     gene            complement(26585..28363)
                     /locus_tag="PLL59_01040"
     CDS             complement(26585..28363)
                     /locus_tag="PLL59_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMY22413.1"
                     /translation="MSIKNILYTTLVVFLLIVVFSAVLSNSGNDSKTYKDELVIHTLS
                     DAQGLNPVTISDATSRRYVMPNIMQSMLYFDLKTMVLKPVLAKSMPEVKEVGDLMEIT
                     YEIRPEATWDNGKSVTVDDVLFSYKSILCPKVNSDNVKSSVDYVVDIKTYPDNPRKYT
                     VICEKNIGAVEGTGYEVVILPEYVYDAKGLLKKYTISELTVENPKCADDKNVKEFADF
                     FNSQQTLRDPAFVQGSGAYVLKSWETGQQLTLERKKNWWGDKINKENIFFEAYPKRLV
                     FVTINNFTTALTALKNEKLDFIFVTPVKDYIDLDNSPKFKKNFYKAEPDQLAYQAIGI
                     NHKDKILSDVKVRQALCYLIDVDQIIEKVIYNKGIRTNSAILPMKKDIINPAIKPFPF
                     DINKAKSLLTEAGWKDSDGDGILDKVIEGKKTNLEITYNYNSGNSLREMVGLLMQRTF
                     QQVGIEFTIKSLEWSLYLDELKKHKCQLWYQGWVTSPGLDDNKQLFHTSAANGGSNYM
                     SFGNAKTDKILDEIRVEFDVNKRKELYYKWQEIEHEQIPYIYLYVQKYKNCIHNRFEN
                     INSGPSAYPGVWFAGFKVKRGYKVEN"
     gene            28593..29885
                     /locus_tag="PLL59_01045"
     CDS             28593..29885
                     /locus_tag="PLL59_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HMY22414.1"
                     /translation="MYKLINLSLLVIFFAFLQACSNSGKKAGDTDGFVYDEKIEKEFQ
                     EKLILVEDGGTVEFPEGKFLFKKDLSMEGKKNVTIKGAGAGKTILSFLGQTEGAEGIN
                     ITNCQNVTIEQFTIQDAKGDNLKLKGCDGVIIRHMNSTWTTGADTSNGNYGYYPVECK
                     NVLMEHCEVSYCADAGVYVGQSTNVIVRHCYAHHNVAGIEIENCINSDVYKNKAENNA
                     GGILVFDLPKLFQGNGRNAKIWDNDCLNNNFKNFGKPGSIVASIPPGSGLIIMATDSV
                     DVFNNRIINNKTVGISTISYHLTGKPINDTTYGPYCTAIYIHDNKFDRSGLSLTEKVP
                     DLSTDLGKLLTATFKGAVDILVDGSSDPKIRDKNGQLPEGKRICVRNNGKVTFGNLNA
                     WKASGIKDIMNYKDDDITKFDCSYTGIRGNNTDPASAK"
     gene            29892..30965
                     /locus_tag="PLL59_01050"
     CDS             29892..30965
                     /locus_tag="PLL59_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HMY22415.1"
                     /translation="MKKITLFSVLSISISCLFLLNQCKTSSNKVNLDINAKPLEKLSE
                     YHFFTGALKDLNPNDKVLPYDINTALFTDYAHKARFVWMPEGTSAKYNKEEALDFPVG
                     TVLIKNFFYENDETNASKGRRIVETRLLIKRADKWDANAYVWNDEQTEAFLNIAGDSK
                     KVDFVNLHGKNIHVDYSVPNKNQCKNCHNLNNVLLPIGPKVRYLNKDFKYADGSKNQL
                     EKWAAVGYLKGYNKEENLTNKMADAFDVKSGTLEERAKSYLEINCAHCHRPEGSANTS
                     GLFLLTKDNNPESWGIMKSPVAAGAASGDNLFDVVPGKPDESILVYRLSENDPEIRMP
                     ELGRSIQHVEGVALIREWISEMK"
     gene            31110..32348
                     /locus_tag="PLL59_01055"
     CDS             31110..32348
                     /locus_tag="PLL59_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HMY22416.1"
                     /translation="MKHLFILVLCVSVFSCKQNKENNTFVVDEKLQKELQEQFILVED
                     GGTIELPEGNYKFSKSLSMEGKKNITIKGKGVDKTIFSFLGQIEGAEGMNFSNCNAIT
                     LENFTIQDAKGDNIKLKGCTDVVIRHMNSTWTTGADSANGNYGYYPVECRNVLIENSE
                     VSYCADAGVYVGQSYNVTVRNCVAHHNVCGIEIENCINVDVHDNLSHNNTGGFVIYDL
                     PGIPMKNGRNCRVYNNIVRENNFKNFAKPGSYVADIPPGSGIIVMAYDSVEVFNNTFE
                     NNKTLGCCAVSYYATGRPFTDSIYTPYCTSIYIHDNIFKRKTMLIPDLSTNLGKLIVA
                     MSKGPADIIYDGNYSKEMSNTDGTLQSQYKSCFRNNGDIKFFNLNAYKSPVIENVLHT
                     YDNNLATFDCSLTNISGNGK"
     gene            32577..33200
                     /locus_tag="PLL59_01060"
     CDS             32577..33200
                     /locus_tag="PLL59_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22417.1"
                     /translation="MLQSQTGGCCSTPNPPSQTPTATGTLYLPRLQPPNPLWDNPSCN
                     STPFYQQTLPNGTTMYPLDVDIASQITFYFKVRVTTIDGCYIQNTYLNKNNRVALSGI
                     GIKINYPTTKPYVVEVWAQADGTFFSCSNTIPGASPEWYCKFANNENGQQIFGEGTSD
                     NPYNLTFHPFVVANIVFAHNNGGNGSGAVEPKFKYNGNVPFGTNVLN"
     gene            33215..33982
                     /locus_tag="PLL59_01065"
     CDS             33215..33982
                     /locus_tag="PLL59_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22418.1"
                     /translation="MKTTTILFSFLLIGMTIACKKEIKNTTDTSKFSYTDDNITPAEI
                     AANFMARADGGSFTVGANGLEGGYNGESIDINAKRTSVDINGRKFTPAANTIWLGRYY
                     VPDYLANTEQEINEIYSYFTNVLRGPKTNFTLYDTQNKKLVGFTIRNSIPITLTIDNQ
                     NYREAPAGSHIVLHWNADSENNLGVAIALSGDKGLRYIHADETGTLDITDEALEIAGN
                     DRISVTLTRGVIMLGKGADGRDYKLTNCISKTEIVYI"
     gene            34079..35182
                     /locus_tag="PLL59_01070"
     CDS             34079..35182
                     /locus_tag="PLL59_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMY22419.1"
                     /translation="MSIESESPEEMGYASIQYNLAESSVRDIYFRDLQINLDEVFLCY
                     AEHRGNIALRNEIVKNEKDLSADDVLVCPSAATALFIISTTLLNDKDHLVVLRPNYAT
                     NIETPRAINCEISFIDLQFESGFQLDIEQIKNAVKPNTKLISLTSPHNPTGVVYDNKI
                     LEEIIAFATERNINVLVDETYRYLNFQRDMMPYYAAKSDKVISVCSLSKAHGTPGIRT
                     GWLITKNKKLQHDFLAAKEQIIICNSVVDEAIALHILQQENYLQKTHEHIRKNFSVLK
                     NWMDIEQHYLEWIRPNAGVVCFPRIKKEYTVDTKKFYTILYEHFKTFVGAGHWFEQAD
                     HYFRLGFGYPTEEELQQGLKNIDASIEASIVSS"
     gene            complement(35193..35876)
                     /locus_tag="PLL59_01075"
     CDS             complement(35193..35876)
                     /locus_tag="PLL59_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22420.1"
                     /translation="MKQLLLALCTVLIFTACKNNTTTAEKSTTENVISEVKEAITNEG
                     LKAAIYQTETVMPGGMGTTSSTVSFDDYGKKSHTVVVAAISFGGRSMNTTTNSLRLDG
                     YVYSWQTGNKSGMKFKIDESKFDPNNLDFSSMNEKLRKELNYKDEGTETVDGKECRVS
                     SFNSEQMKGKIWMWKQVPIKMEMSVVGKSVTTTLKRLDENPSFSAETFALPSDVEFKE
                     MTMPSTAAN"
     gene            36102..36431
                     /locus_tag="PLL59_01080"
     CDS             36102..36431
                     /locus_tag="PLL59_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HMY22421.1"
                     /translation="MHWHELSTETKEQLEQLKLQQMAKLSVDEKVICVTRLEDGFFGM
                     HNTCPHAGAQLHHGHCNKKGIISCPLHGYKFDIRTGKSADGNNYVVRKYQFKIEDNKL
                     WIGWKNY"
     gene            36459..38921
                     /locus_tag="PLL59_01085"
     CDS             36459..38921
                     /locus_tag="PLL59_01085"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HMY22422.1"
                     /translation="MKYTFTEINNIVNGSILQFDEDTMLTLACIDTRQITNAATSLFF
                     CLIGRNNGHQYIDIAYEKGIRNFIVSEEIDFTKYAAANFCRVENTNAALQQLAIYHRQ
                     QFTIPIIGITGSNGKTIVKEWLFQLLSTEKNIVKSPKSYNSQIGVPLSVLLIESTHEL
                     AIFEAGISTSGEMDKLRNIIRPSIGILTNIGSAHDAGFNDRNEKLAEKLKLFENCQQL
                     IYCDKYVDESQLSNLNCQQISFGEQEKSIYRIIHTEKKNSSTIIDIISQRKEKEKWQF
                     IIPFSDDASVENCLHCIIVLLQLGFSAAVIQTKISMLRNLPMRLELKYGINNCSIIDD
                     SYSADFHSLQIALDFYRQQDTHKKRTLIISEFIDSGLSEKEFILRLKSILQHTKFDKI
                     IGVGKAFIHEIESLTGVVKEWYVFESTEKLIAQFNVLHFDNELVLLKGARLFSFEKIT
                     AQLIGQTHATVLEVNLNALSNNLQVYKSYIPKACGVIAMVKAFSYGSGSIEVARLLEK
                     EKIDYLAVAYADEGVELRKNNIQSPIMVMNPDAVDFDRMLEYHLEPEIYSISILQQLI
                     DCIGNRKIDIHIKVETGMNRLGFEASAISELIKLLHQHQNISVKTIFSHLAASEDAHF
                     DNFTKQQIAQYSQIANTFCAAFNYPIKKHLANSSGILRFPEAHFDFVRLGIGLYGVDS
                     ASNIQDKLQVIGKLKTRIAQIKHVQAGETVGYSRKGVVQKAMKIAVLAIGYADGYDRR
                     FGNGIGEVFIAGQRAKIIGNVCMDMCMADITHIDDVKDGDECEIYGKEISIIEQANKI
                     GTISYELLTRISSRVKRLYYLD"
     gene            complement(38974..40218)
                     /locus_tag="PLL59_01090"
     CDS             complement(38974..40218)
                     /locus_tag="PLL59_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22423.1"
                     /translation="MNTRLVLWGEIGTDRKALIAIQLDEETSKIHTYAFPKEMVTKEI
                     QDALFVEWKNGGEYTFPEGAIQWEIDANSDSILPEEVKVERLDVVLQSQHKWSKKLMS
                     SKINQLLEDEVKLLEEKASVVAEYDQTLWDKAKTQWEKIASYQKKNEISWEQTTALKD
                     KINAVFDALKAVKRINHENEDQANNVLVKNYKKRLEDLQAKLIYNDQWKHIADELKKI
                     QAELKDTAIKWNHKRAIYNQINDIFDNLKKYRMTEVISKTQGRISQLNKILHGLKDSI
                     ARDMESYNQQVEKMQHYTRGKLSADELKNRFGYLLDKTKEKEEKAAGILQTITQLKND
                     IEKEKKQEQERAEKLLKRQQEEEQKAQNKAEETAINQAEIQAPISETTANEINHDDKE
                     EIVAVSSAVEAETPTNTSELAS"
     gene            complement(40372..41058)
                     /locus_tag="PLL59_01095"
     CDS             complement(40372..41058)
                     /locus_tag="PLL59_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22424.1"
                     /translation="MTAYKQTTLAFIIALLITISAQAQNTFVTNEVRQFMSKGEQNGI
                     EIILKGSSVNKAKEGLKSLAKKYKAKITSSKKSPETFIDNAAINTVSANTVDIYAIVT
                     PIENGSKVTFFTDLGGAFITSAAYGTQYAGMDAVIKDFAQSQALSAVGDELKSEEKTL
                     KTLEKELKSLKKNKEKTLKEIEKAKAKIATWESDIVRNTNDQAAKQQQISLQQQIIDT
                     VKSKRSSLKN"
     gene            complement(41071..41754)
                     /locus_tag="PLL59_01100"
     CDS             complement(41071..41754)
                     /locus_tag="PLL59_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22425.1"
                     /translation="MKNFIKTTAVAAVLLASATSVFAQSQFTTNEVRQFMSKGEQNGI
                     EINLNGTKPGDAKDAVEKWGKKMKAKIVTDKKNPEIFIDNAQMPTVSANTLDIYAIVT
                     PIENGSKLTIYTDLGGAFVSSAAYGTQYAGLDAALKKFAKDQAIVVVEDQQKAEEKIL
                     KSLNGDLKDLGKDKDGYLKDIEKAKELIQKREQEILKNDADQKAKQQQISLQQQIIET
                     VKQKRATLN"
     gene            complement(41769..44582)
                     /locus_tag="PLL59_01105"
     CDS             complement(41769..44582)
                     /locus_tag="PLL59_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22426.1"
                     /translation="MKKHFFISCMLITIFANAKKWDASYISKLVAKGEIDKVIEFYKT
                     RYDSDKRDPQDAFRIAELYVKKKEYATALSWYDKESQLVYSSKINLFNYATTNRLMGE
                     YQKALDAYLMYAALTGDVNKVMDLANQCERVIRSSALAYNYKLENYTYNTADNEAYIA
                     VLRTNPVYITTKNNITPEDRTTNEIQQIVRAYENFAPPVKAYMNNVPNMTITGLSYTQ
                     DGNNVVFSAIDEKSASKKHKSKLEKIYIADNLGGNFLNVKLLPIDVDGYSLKNPSFNK
                     NGTTIYFSSNMPGGFGGYDIWQTTLKNNAWSTPKNLGKLVNSSADEINPFMPQDGADN
                     ILYYSSNREGGFGGFDIYSVIKTAAIWEGAVLQPAPINSAGDDNSIIYDNETKTGYFT
                     SNRSGGKGNFDVYRYTPFNLQLIVAAKDTFSEKPIDYALVQVIDAGDKLFEGVTNENG
                     ISAFQINKNKNYTLKVSKDAYRPALIKVNSFGKANGDSIFIDVPLKPDSQFSSTKGAS
                     NTISLDNYIVFTGKVISAATNKPAKNIKMRMVNYTTQKLREVDIDSTGRFEIKLLLNN
                     NYKVIFESPDNKVTDELTTLGTARNSIKVRDYLLSGSKLKLLENKVYTIATLPSDIKL
                     SNVLTQEQKIEVANTTIIHEPICQEKIDSLIRVIAEQNPINTNKGASAKLVQQETSST
                     PKKNILEEKKGTKIEASSEVNKGTDVLHVENINSDKQDKVSSQNIIITKTITVDTIAK
                     ISQPLKVENESIALAADMVDNEKSKKIKVLHQKNNDTEIEKVVPDEIKIEQSSSTITS
                     INPTMVETKPQAKLQTPVAAQTNTNDLPKNDNTMVAEMPNIPAAKTDILIAKPEQAAP
                     IAENYYKVQLASYDEGNIQFPEFAHLGKVEEVIAYNRFIYRLGDFDNLEKAKEVLTYV
                     REQGYFVAFILQYNKNKITGIVK"
     gene            complement(44592..47423)
                     /gene="polA"
                     /locus_tag="PLL59_01110"
     CDS             complement(44592..47423)
                     /gene="polA"
                     /locus_tag="PLL59_01110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HMY22427.1"
                     /translation="MNKLYLFDSFALIFRAYFAFQKAPLINSKGFNVSAIQGFLNTLW
                     EIKTKYNPSHYAIVFDAAAQTDRQAEHEFYKANRQETPDDIVASIPYIKEIVKAMNMP
                     VVEIAGYEADDLIGTLAVKAAAQGFECYIVSHDKDLGQVVADNIFLHKPPFMGKPAEI
                     VGVQELIKKWEIKEPKQIIDILGLWGDAVDNIPGVKGIGEKGAKMLIRQFGSIENIYE
                     NIDEVKGAMKEKLIEHKEMALISKKLATIITDAPIDWNENDFQLKAFNKEKLAQLFSE
                     LEFRTLGKKMIGDDYSANDSIKNITNPKTETPKNTAQTSMFASEELDAISTMPPTQKL
                     QATSFGKNINNTTHHYTLIENDAQLQALMQQLNTATHICFDTETTNVDANNCELVGIS
                     FSIKPTEAYYIPIDADRTSAIAQLQHIKPILENDKIIKIGQNIKYDQLVLKWYDIEVK
                     GVLFDTMLAHYLIDADTKHNMNVLAENYLGYTPVSIEELIGKKGPKQGTMRDVPLEKI
                     KEYAAEDADITLQLHNVFKKEIENSHLHKLFYEVETPLIPVLTDMEFEGVKIDIPFLK
                     AYSNEIGADIVKLKDEILNICGVQFNIDSPKQLGDILFEKMGIKYPGKKTKTGQYSTD
                     EETLQKIASEHPIAAQLLDYRELTKLKSTYVDALPSLLNAKTNRIHTTFNQAIAATGR
                     LSSVNPNLQNIPIRTERGRKIRQAFIHKDENHVLLSADYSQIELRIVASISEDANMID
                     AFAKNLDIHAATAANVFNVPLEAVTSEMRRQAKMVNFGIIYGISAFGLAQRLGIPRTE
                     AAQLIEQYFEKYAGIKNYMDSTIHNARKNGYVETLLGRRRYLRDINAGNFTIRGFAER
                     NAINAPIQGSAADMIKIAMINIHKQLKAHKFQSKMILQVHDELLFDVFLPEKQEIQHI
                     VIREMQNALPLQVPIEVSTGYGDNWLEAH"
     gene            complement(47482..47835)
                     /locus_tag="PLL59_01115"
     CDS             complement(47482..47835)
                     /locus_tag="PLL59_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMY22428.1"
                     /translation="MEKAVKEFNDYRAKMNEVILAQQNLTLNRFFNLDMHAYAEGAID
                     AKTKEMLGLVASMVLRCDDCIKYHLGKSYEAGISTAQVYEVFAIANLVGGSICIPHTR
                     RAAEYWQALQSNSCV"
     gene            complement(47908..48240)
                     /locus_tag="PLL59_01120"
     CDS             complement(47908..48240)
                     /locus_tag="PLL59_01120"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMY22429.1"
                     /translation="MENFEFNTEKIEKAAELLRAVAHHLRLKIIKLIHEKKEVNVNVI
                     YNTLKIEQSITSQHLKILRGVDVVRTRRDGKKIYYSLNYERLEAMHRGIDLFEEWTQN
                     RKAAKKKK"
     gene            complement(48374..48706)
                     /locus_tag="PLL59_01125"
     CDS             complement(48374..48706)
                     /locus_tag="PLL59_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMY22430.1"
                     /translation="MKSMTVNNNNHVQPELKIDLQILRKSVLVLRSVNHKLRQEIIQL
                     IDKNGKMTVTDLYIKLRIEQSVASQHLALLRRAGVVVTSRDGKYIYYSVDHERLDEIS
                     RLLSELVK"
     gene            complement(48823..49524)
                     /gene="tsaB"
                     /locus_tag="PLL59_01130"
     CDS             complement(48823..49524)
                     /gene="tsaB"
                     /locus_tag="PLL59_01130"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HMY22431.1"
                     /translation="MHTNEHPIYILCIETANAVTSVAIAKNDICFAVREINTSNKAAD
                     TLHKLIEELLLSCALTFNDIHAIALSSGPGSYTGLRIAAAAAKGYAFALQIPIISIST
                     FQAMLFGIQHRYHQLAYDVYVPMIDARRMEVFTSFFKADGQLVQAYSSLIIDDEFNTL
                     LDKTKKYIFFGNGAFKVPLANIIPTNVVYTEYTPSAADVCALAYKHYTTQQFVDIAYF
                     EPNYTKAVYTTVSKH"
     gene            complement(49524..51167)
                     /locus_tag="PLL59_01135"
     CDS             complement(49524..51167)
                     /locus_tag="PLL59_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMY22432.1"
                     /translation="MKNAITIFCLCLLLNATAADQNRYFEIAKNMELFTQLYKELNTY
                     YVDDVDPNKLMTDGINKMLDGLDPFTNYITGADLDEYQFQTTGKYGGIGARISKIGDG
                     VIITDPYEGFPAQKAGLQAGDLIVEIDGKVATKYNSDEVSQLLKGEPGTKIVVKVKKA
                     ISNEEKTYTFNRQEIKIENVPYYGMVNDKIGYIKLISFTDNAAQEVQDALRSLKKNPN
                     IKGVILDVRGNGGGLLNEAINICNTFVERGVNIVNTKGKIQEANFQYSTTGKPVDIDI
                     PLAVLTDAGSASASEIVSGSIQDLDRGLIIGQNTYGKGLVQVTKPVAYKAKLKVTISK
                     YYIPSGRCIQRIDYSHRSADGIASALPDSLRKIYKTKSGRIVKDGAGIEPDIKTEEEK
                     VAPVTIALFTNNHFFHFANQYKYDHQTLRDGNNFSLSDEEYTQFTDYLKGKDYEYTTP
                     SEVKLNDLKKTIKEDSYSETVQQVITDLESKIKHDKSKDLIKYKDEIKHLLEQEIVSR
                     YTYEKGRIANSLKDDVEIKKAIEVLSDVAKYKSMLSAQK"
     gene            complement(51263..51493)
                     /gene="yidD"
                     /locus_tag="PLL59_01140"
     CDS             complement(51263..51493)
                     /gene="yidD"
                     /locus_tag="PLL59_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HMY22433.1"
                     /translation="MKLKQILIFPFIALIKIYKYSISPLLGENKCRYQPSCSTYAIEA
                     LQKHGLFKGGYLAIKRILSCHPWGGSGYDPVP"
     gene            complement(51740..52345)
                     /locus_tag="PLL59_01145"
     CDS             complement(51740..52345)
                     /locus_tag="PLL59_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22434.1"
                     /translation="MEGTIDSICYYVDAPIHTTLYINNDAEKAFQFYLHEGKYILNID
                     CKNNTATVVGSSLNDEYKEMMRINDSMFKKYNIMHAILYPYNGMDRDSAHLLLQKYLP
                     LCNELSDKHVKLFYETHLSSFLTLEYIYSALKYTFEDLGYDTIEYDIKKLKTLFDRLD
                     PKLKRYKMYDECIEMFNRERVNQTELPKPLFFYDEKINQPR"
BASE COUNT        16962 a         9076 c         8966 g        17539 t
ORIGIN      
        1 agagagttgt atgccaatat tcctgagtca aaaattcgtg gttataaaaa tggaagattc
       61 tcttttaatg taattggtgg cagatgcgaa gaatgccaag gtggtggcat gaagttgata
      121 gaaatgaact ttttgcctga tgtgtatgtg caatgtccaa aatgcttggg cagacgctac
      181 aatcgagaaa cattggaaat aaaatataaa ggaaaaagta tttatgatat tttagaaatg
      241 tctattgaag aggcggttca tttttttgaa cacattcctt ctatttacaa taaaattaaa
      301 acactgaacg atgtcggctt aggctatgtt cgcttagggc aagcggctac aacattgagt
      361 ggcggtgagg cccagcgcgt aaaactttca gaagagctta gcaaacgcga tacaggaaaa
      421 acattttata ttttagacga acctacaaca ggcttacatt ttgaagatat ccatgtatta
      481 ttgaatgtgc tgcaaaaact agtggacaaa ggcaatacta tcgtagtcat tgaacacaat
      541 ttagatgtca tcaaagtggc tgatcacatc gtagatctag gaccagaagg tggccgaggt
      601 ggtggcgaaa tcatagccac cggaacgcca gaaatgattg tcaaaaataa aaaaagcatc
      661 actggtcagt tcttaaaaga cgaattataa tagaaagtga aaaatagaat gctattctag
      721 cataataatt tcacatctac ttcgctataa tttattatat ttaattatag atgaaactac
      781 tttctttcta tcttttagtt ttcaatttcc aattgcacaa tgcaagtgaa acaccaaatg
      841 taattggcat ctggacctgt agcgacaaag attgcaaagt agagatttac aaaaatggaa
      901 atagctatga agccaaatta atatgggcaa gaaaagaaat agatgaaaaa ggaaaaccaa
      961 aattagacat aaaaaatcct gacccaacaa aacgtaattt acctattata ggcagcaaaa
     1021 cactatggaa tgctcaatat aatccagcta ctggctattt tgaaaatgct actgcataca
     1081 gaaatggcag atatttttgt ggcaaattta agctgaacaa caatggctca ctcaccatta
     1141 tcggatatca ttgtaacttt aaatttctta aattttcaga tacatggaca agggtaaata
     1201 cacattagta taacttttta gtataacaat tgcagaactt agtaaaggcg ctttgcttgt
     1261 caaagctatt atctcactta tttatctttt gaaaagccaa tcctatttaa tacctactac
     1321 ttttacatca aacaaaaaat tatgacagca tcgaatacag cacacaattt aaaagtacgc
     1381 aatgttcatt ttaactttga caaagtgcct aaacattgga tattgggaag ttctgtggca
     1441 acacacttta tcaacagtat gcatattgtt ttccctgaag gtgaaaagtt ttttgtacga
     1501 agtgttcgcc actttgctaa agatatcaag caggaagaat tacgcactga aataaacagt
     1561 ttttgtggtc aagaaggtgt gcatgcgcgc gagcaccagc ggttttggga agtaatggaa
     1621 gagcaaaatt taaaaccaaa aaatttcgct aatttcttaa aaactaccat gtttgccggt
     1681 aaatatagct atgaaaaaat aagcacaaaa ttactcaata caatcagtcc gcaattaggt
     1741 gacaaattcc aattatcagt aacaacagca ctggagcatt atactgccat cattgccaat
     1801 gcattatttt tagaaccatt agcaaccaac aaaaacattg cgccacaaat gctggaatta
     1861 ttacattggc acgcatcaga agagatagaa cacaaggccg tttgcttcga tgtactgaag
     1921 gaagtcgacg acagctacct cttacgcgtg tctggcatgg gtgtggcttc cattttgcta
     1981 tggagctcaa tcgccactgg tcaagtatat tttattataa tggacaaaga taaaagcatc
     2041 ataaaaatgc catttgaaat tttcatctta tttaatgcta ttgtttttgg tgaagttggt
     2101 aaaaatttat caaaacattt cttagattat ttcaaaccca attttcaccc taacgacatt
     2161 gacgacagat atttaattga aaaatttttc aaagataaaa cctatgctta gtggtggttt
     2221 tcttcggttt tacttttaaa aaactttaca tgcattttta accatacggc tcttgacaat
     2281 tgaattacat aaggtgccgt aaaaatcaag atagcaaaac aagcaatgag ataatacatg
     2341 gcattgtcta aaaatgcttc tcttgtgtta ttaaacagat aatatatagg caaaatagcc
     2401 aaacacaatg cacaggtgat gatgtaactc atatacatcg caccttgata aaatcctgtt
     2461 tctacttcaa aatcttcatg gcaatgttcg caatgttcgt gcatgtctaa aattttattt
     2521 agttcataag aatgcttgga tttaaacaag cttccgatgc cacacgatgg acatctcaaa
     2581 aaaaatacac tttttatagg cttcctattt gtcatcttaa aattataatt ctattttttt
     2641 gttaattcaa aaaataattc agcaccaagt cttggcttta cagagctcat acaaacgtcc
     2701 atttgttgaa tattaaaatg ctcattaaac aattgctcat actcggtttt actaccacca
     2761 aatggcggac catctttaaa ttctttgtcg aataataaac cagcaagctt tccagtttct
     2821 ttcaacaatt ctttcatctt aatagcatat gctgtgcgaa gatttctatc taatgcacaa
     2881 aaaaatgttt gttcaataat aaaatcgtat tgacctgtat gtttaaaaaa atctccacaa
     2941 ataatcgtca attctttacc tgcatatttt gcaagcttgc ttaataattg ttggacaagt
     3001 gttggagcaa tatcaatgac agttacattt ttaaatccat tttcaagtaa atatatcgct
     3061 tcataggcat tgccgcagcc aggaattaga atcgatttat ttttgcacgt catgctgtca
     3121 aaatacaatt gcagaggcgg agaaacagca ttcaaatccc agcccgtaat tttgttttgc
     3181 catagattgt cccaaaatgt tgcatctaaa tgactaggtg ctttgtcttt actgcacttc
     3241 aaatcgctca taatactaat tctttaatga tgataaaaaa acccattatc aataccaaaa
     3301 taccaaatgc ttttttcaat gttgcacctg ctattttatc tgacaatttg attcctaata
     3361 acattcctaa cactgctatg ctactaaatg acaataccaa tttccaatcg acagacaact
     3421 gcaaatcacc gaaaaatccg attaaagaat tgacggtaat aatgactaat gatgtgccaa
     3481 tagctcgctt catgcagtgt tctttaagca ataccaatgt tggaataatt aaaaaacctc
     3541 caccagcacc aagcaatgcc gtaagcatac caatcaacat cgctaccaaa gttgttttag
     3601 tgtgattcgc atttgcattc ggaatctttg caaagcaatc tacgtcttct tgttgcgcat
     3661 tttttatcat cgacacggac gaaatcaaca tcacaatagc aaataataat agcaaaaaaa
     3721 tgtcttttgt tatttgcaca ttaccaatat taaaaaaatt atttggaaaa taaggcagca
     3781 agacttttct tgtcaaaaac aaacttatca atgatggaat taagaaataa attgccgttt
     3841 tgatatccac taatttttca ttgtactttt taaatgcacc aattaatgca gtaatgccaa
     3901 cgacaaacat cgagtaatgt gttgccgttt gcatgtcgtt tattttaaag agatagacca
     3961 atattggcag tgctaagata gtacctccac ttcctatcaa tcctaatgac aatccgataa
     4021 acaatgccgc tatgtagcca agtatttcca actaaggtgt cgctattttg ttgtaaattt
     4081 acggcttatt gtactacttt tctgtgattt atgttacaaa ataagactgt ttatggtatt
     4141 ggattgatga gtggctcctc tttagatggt ttagatattt gttatgttaa atttgaacaa
     4201 caaaaatcaa gcatttgcta tactatactt caagctacat gtatagcata cgacaaacaa
     4261 caaacagaga aattacgtaa ggctgctcaa ctttccgctt tcgaatttgc cgcattacat
     4321 actgaaatgg gaaaattctt cggctcactt acacatcaat ttattcaaca acaccaaatt
     4381 gaaaagcttg actttattgc ttcacatggt caaactattt ttcatcaagt agctactgga
     4441 tttacaacac aaatcggctg tggtgcacaa attgccgcac agactggaca caaagtagta
     4501 tgtgatttac gaactgctga tgttgcctat ggcggacaag gagctccaat tgtacctatt
     4561 gctgaaaaat atttatttcc tgatttctct ttttttctca acattggtgg catcgccaac
     4621 attgccattc acaacaaaga aaagactaca attcatgcgt atgatatatg tgctggcaat
     4681 acgatgctaa actatttagc aaaacaaaaa aatcttgatt ttgacgacaa tggcaacttg
     4741 gccagaaatg gaaaagtcat taatgaatta ttggaaaaat taaatgctat tcctttttgc
     4801 aagcaagcag caccaaagtc attgggaact gaacatattt atgaaacatg gattccattg
     4861 tttgatgaat gcgatagtac tattgaagat aaattagcga cagcagtaga acatattgct
     4921 atgcaagtcg gaaaggagat aaacaatctt attccgcatc ataattcatc gctttttatt
     4981 actggtggtg gtgcgctgaa tacctatttg atagaacgta tccaacaaca tacaacatac
     5041 aaaacaatag tgcctgatat gctgacagtc caatacaaag aagcattagc aatggcattt
     5101 tttggcttat tacgcattat agagcaacct aattgcttag cttctgttac aggtgcccaa
     5161 aaaaatgtga ttggtggcgc tgtgtatttg ccttaacaaa ataatagtat ataacaatta
     5221 actgattact tttaccttta caccatggtt ttggtgacag gagcttcggg ttttttaggt
     5281 ggcgagttgg tgaaacaact tgtagcacaa ggcgagtccg tgcgtattat taaacgcgcc
     5341 tcttcttcgc ttacgcattt acaatctatc attcatcaac ttgatattgt tgaggctgac
     5401 atcttagata ttccatcatt agaatatgca tgtgaaggtg tcgaaaaaat atatcattgt
     5461 gctgctgtga ttggttatga tagctcttac tataatgcga tgtataagtg caatattgaa
     5521 ggcacagcaa atgtggtcaa cgttgcacta tcaaaaaaca tacaaaaatt aatacacgta
     5581 agttctattg ctgcaatcgg cggcaagcct aatgagttaa taacagaaga aacaaaatgg
     5641 gaaaaaaatg aatggacaac acattatggc attacaaaaa tgctggcaga gcgcgaaatt
     5701 tggcgcggca tacaagaagg cttagtggct gccattatca atcctggaat tattgttgga
     5761 attgggcacg aacataaatc gacaatgcgt atttttaaaa acatacaaca acagaaaatg
     5821 ccattttata ccaacggaat aaatggattt atcgcagtag aagatgtcgc aaacttgtgt
     5881 gtgcaactca tgaatagcga tgtgcaagca gagcgtttta ttgccatcga aggcaactat
     5941 tcttttaaaa catatttcga ttccattgct actgcattgc aggtgtcacc acccaaaaaa
     6001 gcactgaaca aaacattggg caatttcttt ttattattag attatttagc ttctttattt
     6061 acgaatcgga aacgtggact taccaaagaa aacatgacag tatccatgga agcattcact
     6121 tattctaatg aaaaaataaa acaacaattc aattatacat ttcaaccact gcaagaaaaa
     6181 atcaatcaaa ttgctcaaca acttacaagc tatgaacgaa gcaactcgta gaaaaatatt
     6241 aggcgtctgg tttatcattg gcgtaggcat gctattgggt caaatcattt taggtggcat
     6301 tacccgacta acaggaagcg gtctttcaat tacagaatgg aaggcaatat taggattttt
     6361 accacctatg aatgagcaag aatggcagct tgcatttatt aaataccaac aatttggaca
     6421 attcaaattg cttaatacaa caatgacatt atcacaattc aagtctatct atttttggga
     6481 atggtttcac cgattatggg cacgtttaat tgccttagca gccattattc ctttagtata
     6541 tttcatcatc aaaaaaatag ctttaaaaga agatatcatc aaaatactaa ttgctatttt
     6601 attaggtggc gttgctggtg ctgttggttg gataatggta caaagcggat taaaacaaga
     6661 tgccgtattg gtcaatccag tcaatttgat gtcgcatatt gtagtggcta caattattgt
     6721 tgcctatgtg ttgcgagttg gattggaata tttatatcct aaacaaatat tgccttttga
     6781 caacaacaaa aggaaattat ttagcatact cattattttg gtgtttattc aaattgcatt
     6841 aggtgcttta gttgcaggct ctaaagctgc attggtttgc acatcatttc cgctgatgaa
     6901 tggcaattat ttcccgacac tatttggcaa tcacgaaact gatgtacaat taatcttaca
     6961 attcatacac agaaatgttg cctactttat cttcttttat gtcctctttg tgtttgtgcg
     7021 tacaaaaaaa gtcgatgctc aatttcagtt tcaccgcaca cgatatttat tattggcaat
     7081 ggtgttgata caagtaacct taggtgtgct cactttactc tattcacaag gcaaagtgcc
     7141 tgtaacattg ggcgttttac accagttcgg tgcttttcta ttgcttattt tttccattga
     7201 aggtcattat tttatcaaat acagaagtgc aaaaaacgaa aggtaattct actgtttttt
     7261 tcaaacatca atttcaataa taaagatatg cagctcaaag tttcttcgat taccaaccta
     7321 actgatgctc gtttttttag tgccattggc gcacattatt taggtttttg ttttgatgtg
     7381 cttaatgtga ataatataca tccaaaaaaa gcaaatgaga ttattgcatg gttgcacgaa
     7441 cctgttgttg ttgctgagtt tggcgcacat caaactaaag aagaaataga atatattgca
     7501 tcgcagatag acattagcga aatacaaatt cctatttcca acaacgatat cggcaactta
     7561 cattttgata aatttattga aatagaattg acagacattg aaaaaacatt gcctgcatct
     7621 gattattatg tattaaaaac ggaacaaata aacacagaag atttagcttt aaaagaattt
     7681 attgcacatc acaaagtctt tatcgacacg gcattcacca aagaaaatat tgaagaaata
     7741 gtggcgaaac tacaacctta tggcattcaa attacttgta aacgcgaaga aaaaacagga
     7801 ttaagctccg ttgatgaata cgccgaaata acagcagcca taggcttttc atgagattgc
     7861 aaattattgc aattggaaac acaaatattt aattgtgcgc ttatctttta aatgcatttt
     7921 ctcgtaatag gtttgaatgc cgtgcaattc atcattcttt tgcatggcat acacatcact
     7981 gtaatctttt acaatcgaag agggaaattc tgctaaagtt tctttagtga atgcatacaa
     8041 cacatcgctg tctgtcttca aatgtattaa accgcctggc tttaatattt gcttgtaaat
     8101 gggtaaaaat ttggtagaag taagtcgctt tttcgcatcg ccatcacgca gaaatggatc
     8161 ggaaaatgtt atccatattt cgtcaacctc agcttcctca aaataattat acaataaatc
     8221 aatttgctca cgaataaatg cgacatgctg caagccttct aaattagcaa tacgagcagc
     8281 gctccatata cgattgcctt taatatccac accaataaag tttttttcag gaaaaagctt
     8341 tgccatgcct aatgtataat cgcctttacc acaggcaagt tctagtacaa tcggattgtt
     8401 atttttaaaa taatcagcat gccatttgcc tttcaatatt ggattttttt gaaaaactat
     8461 tggattatca gccagctcgg caaacttctg taattttgat ttagaggcca cttatttttt
     8521 ttgcaaaaat acatttttgc aatgatatgc tctgctatca ataagtgatg ctgtgttctt
     8581 tttgttgaga ataagataaa atctgctgaa taattgcatc attaggaccg tcgcctccgt
     8641 gttcatcaat cgcgtttttt gtggcacata attgttgata ctgctcttgg agttttgtat
     8701 cacttgctaa aagctgttgt acaatggcat tttcatctgt atccgtttct tggtaaagat
     8761 gtttaataag caaatcgagt gtagaatttt gttgcataaa ttatatatta tttacactgt
     8821 ataacgaaag ttagtacata ttatttgaca tcacaagaaa ttttaatata atttttgcac
     8881 tttttgtgct tagtctgtac caccagtacc tttaaaatag tcgtctttaa aacgaaataa
     8941 cacattaaac gttgtcaaac ttccgtaaat tcttgttata tattgctgta attcaacctt
     9001 ttctgcatct gacaatttag aagcattaat tttttgctcc attacacgta agcggtcacg
     9061 agtcatcacg attttatgaa aaaatccttc aatcggaatt tcttttgctt gcaagctttt
     9121 atctttaggc tgtaatatca agttgccacc ttcccatttt tcagcaattt tgcaatcttc
     9181 aatcggttca gtaagtggcg caataatatc cgatagtact gattttatgt cttctaaaga
     9241 aacgctattc gtatcttttt caatcatttt aatcaataca aaaccttcaa atcttttgga
     9301 tatttctttt tctccatgct caggaaaata aaggttgtaa acatactcgt cgctatcatc
     9361 aataatgatg cctttgccaa atgttggatg ttcgattttg gtgcctatgg aatatgctat
     9421 catacttttt ttgacgaaat taaaaaacac attttacttt tccaattgta caaattctca
     9481 ctcacaatgc ccgaatgcta attttttatt gatattcatc aatacattct ataattttag
     9541 cgtataaaag ttagtaaaat gaaaaggtat ttatttttaa gcattggatt tattttttgt
     9601 tttaatctgc aatcatttgc gcaatggggc agcattggcg atcgcatcgt tgatggattg
     9661 agtaataaag cacaacaaaa aatagaaagt gaaattagca atggcgctga tagagcatac
     9721 gagaagtcaa aaagcagcgt aaaggaaagt gtaaagaaca gtggtagcaa atctaaaaat
     9781 tctaaatcat cttcaagtaa ttcgaatgac aataataatg aggataatga tggacaaagc
     9841 aacagcagtg cctcctcatc aaaaaataca tcctcatcaa gtagcttaaa ggcttatggg
     9901 aaatttgatt ttattcctgg tgaaaaggta attgctgaag agaatttttc gcaagatgct
     9961 attggcgatt ttcctgcgaa atggaataca aatggcacag gcgaattagt cacaattgat
    10021 ggtcagcaag gaaagtggtt gaaatttgga ccagaaagca ttatctatcc tgagtttgta
    10081 actaatctac ctgaaaattt tactgtagaa tttatgttgg cgagctctca gccttttagt
    10141 tattactcta ctgcctttaa tttctttttt gcgccattgg ctaatcctgc aaagaactat
    10201 gtcaactgga aaagattcgg tggcgacaaa aaaaatggtg tattggtggc attgcaaccg
    10261 gaagctgctg gcggcaatgc aatgggacaa aaatggataa atacatttga tgaaactggc
    10321 gatgagatta taaaaaacca aactgacttt cctgaattta atcatgagaa taaaaacgtg
    10381 gtgaaaattt ccatttggcg acaaaaacag cgcttacgtg tatatgtgaa tgaaacaaaa
    10441 atttgggata ttccaaaagc atttagtgca acggctaaat ataattttgt tgggtttaga
    10501 acagatggat atcacaatga taatgacgct tattatttaa gcaatttacg tgtagctata
    10561 ggcgcaccag atacacgaca taaatttttg gaaatcggaa aatactctac tacagggatt
    10621 aagtttgatg tcaacagcga taaaataaaa ggagaatcgt atggtacctt aaaagacttt
    10681 gctgccgtga tgaccgagaa tccagatgtg aaagtgaaga tagttggcca caccgacagc
    10741 gatggcgatg atgccagtaa tatgacgctt tccaaaaaaa gagcagatgc cgtaaaagca
    10801 gccttaaaca aagagtttgg tattgacaat agccgcatgg aaacagaagg caaaggcgaa
    10861 tcacagccag cagaaccgaa cacaacagca gaaggcaaag ctaataacag aagagtggag
    10921 tttattaaac tttaaaagat aacatcatga aaaaatcaac attaattctt gccgtgctta
    10981 tttcgatatt ctcggtaagt tgcaaaaaag agaatctcgg tatagattat agtaaaataa
    11041 gagtaaaaca aatagcaaca aataacaacg gcagtagcat ggataccata cgtttttttt
    11101 atacaaacga taatgtcaaa acggtgacca attactctgc aaatcctaca tttctatata
    11161 cgtttaatta tattaaaaat ggaaataaat acgatgtatc aagtatatat ggcactgtaa
    11221 atacccacga aggattttat aatataaatt cacaaggatt tatagatagt tctaggttta
    11281 cgaatataat tacatctgca tttaacaata gggataagta ctattataat agcgatgggt
    11341 acaacacaag atcaattacc aattataata catatgaaaa tgacgtaaca aaatattaca
    11401 ctgtggaagg agattttaga tattggatat acgacttcaa acattttaca acaccagcaa
    11461 gctcatatat tgactctatt gcattcgact attataatga tgaaaaccat gttgcatttg
    11521 aatattcatt gcaagataaa tttgggaaat tgaataaaca cctcgtcaaa aaaaggtaca
    11581 attatgacag aacaacaggt atattaaaac gaactataga ataccagtat gaattagata
    11641 gtaagggttt agttacacgc agaattagta gttactataa tcagccagga aacatattga
    11701 gttctacagt ggattcaagg tacacctatt atgaataaac aagctaatac actaaaaaat
    11761 aacatcatga aaaagacaac attaattctt gctgtgttta tttcgatatt ctcggtaagt
    11821 tgcaaaaaag ataaacttga tagcgattgc aatatcgcat caattactat aaatgacaat
    11881 ccatcattta aatctacgtt taaatatgac aaccagaata gaatttctga atacacagaa
    11941 gaaggtggtg ccgttgttta taaatttgaa tacacaggat tgagtggaaa gttgaaatta
    12001 tttttagctg acacattata tgaaaccaaa aaccttacgt ttgatagcaa gggcaggttg
    12061 acttcttttg aagattattt accatttgga ggtgatttgc tgagatattt ttatctattt
    12121 acttacaata gtgatggtta tttagcatca acgatacaaa cattgactgg cggaagctat
    12181 gatagttatt acagagattc attaatttat tctggcggta atcttattaa aaattttaga
    12241 aaacacagaa ataatactat ccaaacaaca acagaattta gctatggaag taacactaat
    12301 aagcattgga atttttattg gaatgcattt acagagccat ttagtatttt atctggttat
    12361 acttttttgt atccattgtt gggtaaaact agcactaatc ttccgactag tgttgtcgtt
    12421 acagaaggta gtttcataga taatttttcc tatgcatatt tcataaattc ggctggcaat
    12481 gcaacagaat ataatgaaat gcgatccaat acagtgacag gaaatacgtc ttcaaattac
    12541 aagcttacct acacatgtga ttaatcaaaa aaaaatttta cttttatttc tttctatata
    12601 aacagcattt taaaaaataa aacatgaaaa aaataagcac taatatttgt atcgttttaa
    12661 gcattgccct tttctttgtt gcctgcaaga aagatgataa tggcaaagta cgcaagcaac
    12721 acatttcatt aatggcagat ggcgttgcat tttctgtaac tactgatggc gtggacgtag
    12781 aagaagccga cgcacacatt cgcgatggca ataaattcca actagatgcg accaccagca
    12841 cgaaagactt ttccatcaat aataaaaatg tgccaagcac gggcaattat gtaatgaatg
    12901 gcgcaggtac tgtttttgat tttgctcaat gggaagatgg ccctgcatta tttagctcac
    12961 tcggctcacc taaatcgcat tttacgttta atatttctaa tattgctggc agtccttcag
    13021 attatgttcg ttatgtagaa ggcacttttt ctggagtcct ttacaataac acacaaacag
    13081 attctgttat tattacaaat ggtcaaattc atatcataga ttaaagaaaa taagctatct
    13141 ataacgccat ttacctacgg taaatggcgt tattattata aaaattgaat ttctttaaaa
    13201 gcaaaacttt ccttgcctgt catagtttca agtatcaatt tgccgaactg gtctatgccg
    13261 ataatttttc cttcaaaaat ttcctctcct tttttataaa aagtccattt ttctttttgg
    13321 tacaaatttg ccaaatattc tttatctaac acttcattgc gttgttgttt tagcagcaaa
    13381 taatactttt cgatatgctg caagatatca ttcaaaattt ggtttctgtc taaagatttt
    13441 tccaaaacca actgcatgct cgtagcatta acaccatcag caaatacaca ttgattgaca
    13501 tttaaaccaa tacctattac ggaatgcttc aaattcatgc cagcaatcgt attttctatt
    13561 aatatgccag caattttttt gttgttgaca aaaatgtcat tcggccactt cacttctaca
    13621 attggcttat ttttttcaag tcggaaataa gcatctaaca ttgcccaaac gccaagtgaa
    13681 atcgctttat tcaagagaaa ttgtgccgtt gccggtaaaa aagaggtttg ataaataata
    13741 ctaaaagtca ggtttttatg agcttccgtt tgccatttat taccagattg tcctcttcca
    13801 gcaaattgtt catctgcaaa aatgacagtt ccatcaattg gactattatt tgcaatatga
    13861 tttttagcaa aattattagt agaatctact gaaggcaagt gcaacaggaa ctttccagtg
    13921 aaaaaagtgt tatttacatt aatttccaaa atttagtagc tttgtatgtt tgagtgaaat
    13981 tataaataaa aaacatttgg aaaaagtaaa aactaagaaa aaggcaaaga caataagcgc
    14041 tgatgcttta aacgagctgg ttgtatctct cattcaggat aaaaaaggga acaatatcgt
    14101 cagtttagat ttgagacata taccagaagc aatcaccgat ttcttcgtaa tttgtcatgc
    14161 actttcaaca acacaagtgc gcgcaatatt agatcatttg gaagaagaag tcaaaaacca
    14221 aacatcaata aaaccctatc acttagaagg tcgtgctaat ggcgaatggt gtttgataga
    14281 ctttggcgat gttgtagcgc atattttcca agaagagaaa agagattttt atcaattaga
    14341 agatttatgg agtgacgcaa ttattaagca ttattgacca tttcaattat taacaattaa
    14401 aatacattta attgcgtacc ttaaacaaaa tacgcacgaa cttgtattaa gaacatcata
    14461 caataaaata tacaatggat tttttaccag aaaacgaaga accgaaaaaa aagaaaggtg
    14521 gatttaacag ctattggctg tttgcaatat taattgttgg tttattagct gcacaatttt
    14581 ttagcttaaa ctttcaaatt aaacaaatct cagaagacga gttttttaga aaattagtgc
    14641 cttctggcga tgttgcaaaa atcacaattg tcaacaaaga attagtagaa gtttttatca
    14701 aagaagattc tctaaaagca aaaaagcaat atgctgatat aaaactcaac aagcttaaat
    14761 ctattaacaa aggaccgcat tattctttcg aaatcacatc gattgaagcg ttcaaacaaa
    14821 agctagatgc catacaagcc aacacgcctt atgaaagttt gataaaagta aataacgaaa
    14881 tacgaaaagg tatttttgat atcataggtt ggttgttacc gtttattcta attattggaa
    14941 tttggatgtt cttaatgcgc aaagccagcg gtggcatggg cggcgctggt ggacaaattt
    15001 tcaatatcgg aaaatcgaaa gctaacttgt acgacaaaga catgaaagta aatgttacat
    15061 tcgacgatgt tgcaggatta gacgaggcaa aagaagaagt aaaagaagta gtagattttc
    15121 taaaaaatcc gaaaaaatat acagcacttg gtgcaaaaat tccaaaaggt gttttattag
    15181 taggtcctcc aggaacaggt aaaaccttac tcgcaaaagc aatggcaggc gaagcacaag
    15241 tgccattctt ctctatatca ggctcagatt tcgtggaaat gtttgtaggc gtgggcgcat
    15301 cacgagttcg cgatttattt aagcaagcac gcgaaaaagc gccttgtatc atctttatag
    15361 atgagataga cgcagtaggt cgtgcgcgcg gcaaaaatat gatgcaaagc aatgatgagc
    15421 gcgaaagcac actcaaccaa ttattagtag aaatggatgg ttttagtggc gacaaaggca
    15481 tcatcatgct ggcagctacc aaccgacctg atgtattgga ctctgcatta ttacgtcctg
    15541 gccgcttcga cagacagata tcaatcgaca agccagacca aaaaggtaga gagcaaatat
    15601 ttaaagtgca tttgaaaaaa ctaaccaaac tcggacctga tgttacgcct gaaaagcttt
    15661 catcgctgac gcctggtttc gctggtgccg aaattgccaa cgtctgcaat gaagcagcat
    15721 tagttgcggc tcgcaaagaa aaaacacaag tagaagttga agactttaat gaagctatcg
    15781 accgaagcat cggtggttta gaaaagaaaa ataaaattat ttcgccacaa gaaaaagaaa
    15841 tcattgctta tcacgaagct ggtcatgcta tttgcggttg gtatttagaa catgcacatc
    15901 cattattaaa agtaacaatc attcctcgtg gtatcgctgc attaggttat gcgcagtatt
    15961 taccaaaaga acaatactta tacagaacag agcaattgat ggacgaaatt tgcatgacat
    16021 tgggcggtcg tgctgcggaa gaaattgtat tcggtaaaat ttcaacaggt gcgcaaaatg
    16081 acttgcaacg tattacgcaa ttagcctatg cgatggtgac tgtttatggt atgaacgaca
    16141 aagttggaaa tgtgtcattc tacgatcctc agcaagacac aacatttacc aaaccttact
    16201 ccgacgaaac agcgaaagta attgatgaag aagtgcgcaa attaatcgac actgcttatc
    16261 agcgaacttt aaatctattg acagaaaaac gcgaacaatt aaatactata gcaaaatcat
    16321 tattggaaaa agaaattctt ttcaaatcag atctagaaga gttaattggc aaacgctctt
    16381 tcgatgaaga gccattacca gtggctattt ccatagatta acgctaatac attattcata
    16441 aagagcatcg atgattacga tgctcttttt ttttgtacat tcgttggtga gcaataaaac
    16501 aattttaaaa atttatagca tcgctttgct tactacaggc atgatgtttt tggttttttt
    16561 gtatttaaat caatcatttg atggtcactt ttggcaatcc atgcaacaga gcaaatctgc
    16621 cttatgtgcg gagtattgcg aactcgacca gccaacccat ttttttaggc aaaccattaa
    16681 ttcctattca aatttgatgt attttttttt gggtgtcatc gtgattgcag tcgctatata
    16741 tgaccttaca gacaaacgag caaataaaaa tccactacaa cgatttccag cactttcttt
    16801 tttatttggc atttgtttgc tgtatttatg ttttggcagc accttttttc atgcatcatt
    16861 gacgtggctc ggtcaacgtg tggacatgaa cgcaacctat agtatttgtt taacactgat
    16921 tggtattagc agctatcgtt attacataaa acaaacagaa aatattgcga ataaagcagt
    16981 aattattata ggcctaatta gtttgatttt tttatttatt ccattgcatt tgcttgttag
    17041 cagtagcata ttattgccat tactaatcag tatcgctgtt ttttttgtgg ctaaaaacta
    17101 cacgaacaat aaggagaaat ataaattaca ttttgcaata ctcagtctgc tattggtaat
    17161 tggtgcattt atacttcgaa cattagatgt aaaaaaaata gtgtgcaatc caacttctat
    17221 ttatcaagca catgcattat ggcatgtgtt tacaggcatg agtgcatttt tattatattg
    17281 gttttaccga agcgagcaag cctaatcatt tacaatttcc accttctttt atgcgctgat
    17341 aaaatttttc gtattgtggt aaaatcacag aaatatcaaa cttctgtgct tgcgcgagtg
    17401 cattcttttt aaattgcaat aacatacttt catcttgcaa aagcgacaag gttttatctg
    17461 ccattccttg tatatcacct acatttagca aaaatcctgt ttcgccatct atatttacct
    17521 ctggtattcc acctgcatta cttgaaacta cgggcacttc gcaagccatt gcttctaatg
    17581 cagccaagcc gaaactctca ctttcagacg gcaataaaaa cacatcagca atagccaata
    17641 attcttctac tgcttcttgc ttacctaaga aacgaacatc catgattgta cataaattac
    17701 gtgctaatgt ttctgcatat tggcgctctg gaccatcgcc aatcattaat aatttagatg
    17761 gtatttgttt ggatacttta caaaaaacat gaatgacatc ttccactcgt tttacttttc
    17821 taaagttaga agtgtgtaca ataatttttt cgccattcgg tgcaattgct tttttgaaat
    17881 gctccttatt tgttttttta aaacgagaaa agtcgatgaa attaggaatt acttcaatct
    17941 cattcttgat atcaaaatgg gaaaatgtat cttgacgcaa gctttcagaa accgcagtaa
    18001 cgccacaaga ttgattaatc gaataggtga ccacaggctc gtacgtagca tcacgaccta
    18061 ctaaagtaat atctgtgccg tgcaatgtag taatcacagg aacattcatg cctttctgca
    18121 ataaaatttg tcgtgccata taggcagctg atgcatgcgg aatagcataa tgtacatgaa
    18181 aaatatccaa ttttgcattt atggcaacat cgactagctt actggctaat gctgtttcgt
    18241 acggagcata atcaaacaaa ggataatcat tcaggcgaac ttcgtgataa aaaatatttt
    18301 cgtaaaaagt atccaacaat cgtgcaggct ctttatacgt aataaaatgt atgttgtggc
    18361 ctttcatcgc taggcctttt ccgagctcgg ttgccaccac gccactgcca ccaaatgttg
    18421 gatagcaaac aattcctata ttcatagtga tgcaaatgta gtaatgtaat aatgaaattt
    18481 gggaataata gttcaattat tatccaaaaa acaaagcaaa gatattgcag gattcacaaa
    18541 ataaacactt tatacacttc gttcaagtgt actctatgat tacaaaacaa cactatttta
    18601 attgtttatt catcttcaaa ccacattcta aaaaagagat ataatttcct ttatctttag
    18661 aaaaatcgta ggcaactggc tggtttaata attgttcttt tgtattgatg atcacataaa
    18721 gtggttgtga gtttgtttta aaatgctccg cctgaaatgc gctccatttt tgaccaactg
    18781 tttttacttc gcgttctttt cctgttgcag ttgaaacaaa ccattcattt tctggcaatg
    18841 cttttctatc atctacatac aaagaaataa ttacatactc ttttaagcgc tcaattactt
    18901 gctcgtccgt ccatacatta tcttctatct ttctgcaatt cacacagcca aagcctgtaa
    18961 aatcaagcaa cacaggcatg tcgtgttttt ttgcataagc tatgccttcg tcataatctt
    19021 tgaaatgaac aaatttagtt tcgttcttag ttttaaaatt ataagacgtt ggcggaataa
    19081 tgccactcaa taagccaaca ggttggtagt taattaaggc atagattaaa taaaatgcaa
    19141 acaataaagt agcaacgcca agtgatttaa ctattgtgtt gtgttttgtt ttaaagaaaa
    19201 tacctaagca ataaaatgcc attatcacaa aaatcaaaat ccaaataatc ataaatggtt
    19261 cgaagcgcaa gatattccaa ttcatggtca tatcggcagt tgataaaaac ttaaaggcaa
    19321 gtgctaattc gataaatcct agaataactt ttactgttgt gagccagcca ccagactttg
    19381 gtaatgaatg taaccattgc ggaaacaaag caaataaagt gaatggtaat gccaatgcga
    19441 ttccaaaacc tagcaaacca aagaaaagcg atgaaccgcc cattactaaa agtgttccaa
    19501 taattggacc tgtgcaagaa aaagatacta aagccagtgt aaacgccata aagaatacac
    19561 ctaaatatcc gcctttgcca gaaagactat ctgttttatt tgcccatgaa gatggtaaag
    19621 ttatttcgaa caaaccgaag aaagacaaag cgaaaatgat aaaaacgaca aagaacaaca
    19681 agttaaacca cacatttgtg gacatagcat tgagtgcatc tgagccaaaa acactcgtga
    19741 taattaaacc taatgataca taaataataa taatagaaaa tccatatacc aatgcttttg
    19801 aaattcccgt tttttgttct tttccgcctt ttgtaaagaa gctaacggtt agtggtacca
    19861 ttggaaatac gcaaggcgta agcaaggcca ataagccacc aataaagcct gcgataaaaa
    19921 tccatagcca gctttttgtc gttgtttgcg catctgcact gcatgcattt tttgcagttg
    19981 aaaaatcaaa ttttgtagat gcaggaactg tcgatactac cgcagttgct gttgtatcgc
    20041 ctgtttttgc ttgtgataat gtatcttgtt tttttacagc tactgttgtt gctgctgtgc
    20101 catcaaatga gatagcaaat ttctctggac cgtaaggcat gcacccaaat tcatctttgc
    20161 aaaccatacc atcgaattcg ccttttaaca ccgctttaga atgcttgagt tttacttttt
    20221 gtacataagt tacatcaccg agaaaagtag ttacatcgac gccaaacagt tcatcaaaag
    20281 ctgttttctt tttaccaatt tcttctacct ttccaattaa ttcgatatca ttatttttag
    20341 caaatcggaa ttgtgtagga agtggtccac cttcttttgt aaattgagaa taagtatgcc
    20401 aatcttcttc gagcgtacaa atgaatttta ctttgtattc attttcgcct attttttcga
    20461 cagcatgtgt ccatgtcaaa tgttgctcaa atgctgcatt ggataagagt ggtgccaata
    20521 acaatagtaa aggaatccat atttttttta gcgctaaagt ttgcattgca tcttgcaaaa
    20581 acgatggtat tgaccaatta ctatgtatca ttattaatta tgtatttact aaattcaata
    20641 agctttcaaa taattttttt ccgtcggtat tgcctaatac ttcttcggca gcacgctcag
    20701 gatgtggcat catgccaaaa acatttctgc cagcattaca aataccggca atattttcta
    20761 ttgaaccatt tggattagct gcttcgttaa tgtttccaga tgcatcacta tacctaaaca
    20821 agatttgatt attttggttc aatgattgta aggtttgttc atcagcatag tatctgcctt
    20881 cgccatgtgc tattggaata ttcaaagctt gctgtaaatc taaatgacgt gtaattgcgc
    20941 tatccttggt ttgtggcaat aaatattgat tttggcaaac aaatttttga tgattattca
    21001 tcagcaaggc gcctggtagc aaatggctct cgcacaacac ttggaagcca ttacaaatgc
    21061 caaatacata accaccatta ttcgcatgtt ctattacctt ttccataatt ggcgaaaagc
    21121 gagaaatggc accacatcgc aaataatcgc catacgaaaa accaccagga atcatgatgc
    21181 aatcttctgt acttaaatct tgcaaatgtg tgtctttgtg ccacaatatg cgtgtttcgc
    21241 attgtaacac tttactgcat acatgaatta catctgcatc gcaattagaa cctggaaata
    21301 taactacacc gaatttcata gaaatttaga ttttgcactg cgattcataa tgtatttttc
    21361 acaatgctga tgacaaaatt agtttactgc tcttataata tttgtaacaa ttattacaat
    21421 cacaaagata gcattcagaa tttcgccaat acgcttttta cgaattctta acataagtaa
    21481 ggcaagtaaa caactcattg gaatcaacaa gactgaaatc gcggctaagt cattgagttt
    21541 attgcttata atcacaaata aaaaacctat aaatgacaca aaaagcatgt ataaattttt
    21601 tcttcgacga aagccaatag aataaaaaat atctcttata ctaatcaatg aaaagaatag
    21661 atacaatatg acaattatta acatcgctat atcaatttta cttttcggca atataatttg
    21721 tggcaatatt gagaagttga aattagcgat attcaaaaat gtatccgtta aataactgta
    21781 tgacaatgcc atataaacag gaaaaagaat cccaaatatt agcatcagat actcattgaa
    21841 ccgaaatggt tttaacacta ataaaataag aaaaagaaaa ggaataaaaa ctataaaatg
    21901 cacgtctatc aggcacaaca gaccacctaa agcacctaca ttaaaacatt ccaaacgcgt
    21961 gctttctttc acggttatag tgatgaaagt ttgatgtaac attaacaaga taaaaacaac
    22021 aatcgtgtat atactaaata cattaaacgc tgagaagcac gagctcagca gtgtaaaata
    22081 caaagcagga agcatcgtac tgctttcgtg atagtctgac ttgtgaaaca aataattaaa
    22141 ccaaattgct tgtacaaata agcaaagctg cgctaataac acattccacc aacttatcat
    22201 tggaaaaatt atatcgcatg aattccatag gtttgttact ggaaaaacag gcacaacacc
    22261 aatgataaaa aatggtattt ttagcaatat aaataaagca aaaaaagcaa ggatagcagg
    22321 cggttgttgt gatttgaaaa aggaaagcat attaaagaca cctacttatt ttattaatat
    22381 tgaaagagta caaattgaag atttcgtttt tgctcgctag gaataactat cgagttagca
    22441 tctaattgtt tggcttttaa tggcaccatc accaagtctt gtttttcgct attcataaca
    22501 ctattgcgct tactaagtcc gttagcatta atagaatatt ctacaaaatt tcctatcgta
    22561 taaaagtctt cattgtatag gaatttaagt gcattattgg cttcaaaaaa tgcaaaagaa
    22621 gagtaatatc catcgtcatt ttcagatact tgcgctttcg gcatatccgt ttgccaatct
    22681 aatgtaccat cagtattgat ggagaacaag ctaatattat aaaagaaatt ttgatcgatg
    22741 tagcgcacag ctggcatata tggtgtggca ttataatagc cataattatt attggcaatg
    22801 cgttctgttc tggtgatttt attttcacct tctgtaatga ctacaaagcc gccatcgcta
    22861 cgcggaatta tacgctttgg cttaattaag accgctttgt ccgtccatgt tttaaactct
    22921 ttggtttgcg actttgccag catagcatca ctaaaaggca gtttactgtt gagcaatacc
    22981 gtattcgttc taaagtcgat aatttggtag aacatgccga tatcagactt gtttgccgta
    23041 cttttatagg cagctacaac gtacacaaca tcacgttttg agcttacttc cgtaatcaaa
    23101 tttttaaaca catgatcact ttgctgcatc acttgctctg caatagtatt cgttgttcta
    23161 ttgaatagga acatcgtgta tttgttgtat gcatagtcat cattgctgtt attttcgtca
    23221 ccagttacgg ctattacatt gccattgtca tttattttta tcgattttaa agcaatattt
    23281 ccaccatctg tattggtgaa tatgtttcta tttagaatct gtaaggaatc atttaaaata
    23341 gtgaatctga caaaatatgc tgcttttgtt ttaagcatat taaatgtcaa tatctttgat
    23401 ttatctggag aagttttgac ataaaatgcc tttccttttc caatacttat taatggaata
    23461 gaatccaata aaatagactc cacaggaatg cttagttttt catctaatac ttttaatttg
    23521 aggtattgat aattttctga attggtggta taaaagacca atattttatt tttgagtaaa
    23581 ataaaacttt ctactgttgc cgcctttcct ttaaatggta attctctacg attgcttact
    23641 tttaattttc catcatatgc aactaattcg ctctcatttt gaccaaagta atgcactaac
    23701 aaaccaatat cattttttcc taaaatttca tagtcagaaa atcggttaga gactttttcg
    23761 gcagaagatt taattattgt ttgtgctgta gtttgatgat tgaagcaaca aaataagata
    23821 aaggcgaaac aagatagcaa agtagttctt gggttgctta ttgttttatt atcctttatt
    23881 tcgctactat atcgcttcat tatacgttat tttttttgtc ttggatccaa tgcatctgtt
    23941 aagccttctc caattaagtt aaaaagcgta acagtgacga agatggcaaa acctggaaat
    24001 atggctaccc ataattgcga cctgccacct tgtgccaagg ataacaattt tccccaagtt
    24061 aatgtttccg gcggaatacc taatccaata aacgataaaa acgactctgt tagtatggct
    24121 gctgctatac caaaagcgat ggtaattaat acaggagaca aagcatttgg aattgcatgc
    24181 tttaataaga tgcgccattt cggatatcct aatgcttctg atgcttcgat atattctaag
    24241 cttctaattt ttaataactc tgctctgata aatcttgcaa tttcagtcca tgaggttaaa
    24301 ccaatgacaa ccattaccaa aaatatactt ggtttagaaa ttgccactac tgcaataatt
    24361 aaaaataacc ttggaataga taccaatact tcaatcatac gctgcacgat gatatcaatc
    24421 ggcaagcttg ttttgggtgc caaaaatggg attttcttaa gcactcgcga cacgagatag
    24481 ccaatgagaa tgcatgcaaa aaaaacggca atacctgtaa acaaaccacc aaccgcatta
    24541 gcataaaaca gacccaacat ggagcaaaga atgcccatta ataaagcagc tctggaaatt
    24601 tgtatgcctt tatctccaaa aaaaccagct aatgaaccga gtaaaatacc aataacactg
    24661 gcaatcgaca tcgatatcaa accgatgata aatgcgatgc gtgtaccgtg gattaatgct
    24721 gataatatat cacgtccgat gccttctgtg cctaaccaat ggcgccatct gattgaagga
    24781 acatcttgtt taccataggg cgaaacgaaa ccagagttta aaatatcttg cgtagtcggt
    24841 gaataaggta ttggtggcct aatagcaaag tcaaatttgg tttctgacca aataatatta
    24901 ttcagttcag gtggaaattt tcccagcttg agttttactg catattcttt caaaatggga
    24961 aaataggttt tgccttggta ggtgcaaact atcggttttt cattcgccaa gaaatcggca
    25021 aatatggcta tcaccagcat aaatagcaca acataaaaag aaaacaaagc gcgtttattc
    25081 tttttaaatt ggcgttttac atacgaccaa taatcttgtt tctgttcttg atttgtattc
    25141 aaagtcgttt attttttgtt ataagaaatt cttggatcta ctacagcata taaaatatca
    25201 gccactaaat aaccaactaa agttaagata gcggaaaaca tgactatcgt atatacaact
    25261 ggataattac gggcaactaa ggcttcatag gcggtcttgc ccatacctgg aatggaaaat
    25321 attaattcta atgcaacagc accacttata gccaagggaa acacattggc aaacaaagtg
    25381 ataattggca ataatgaatt tttgatagca tgtttccata atactttttc ctcgtctaat
    25441 ccttttgcac gtgctgtacg cacataatct tgcgacaagg aattgaccat tgcgccacgc
    25501 atttgccttg aaataaatgc taagccacca taagtataac agataatggg caaagtaaga
    25561 tgccaaacat aatttgtaaa cttagtaaag aaggacatat tttccgttgc ttgcggcaca
    25621 ccaaatgatg ggaaaaaatt gaaaaaatct ccgccgccaa aaaacataat taacaaagta
    25681 gccacccaga aacttggcaa tgaatacaaa ataaataatg ttgttgatat ggtttggtct
    25741 ttgatgccac ctttgttcgc tgccgataca atacctaatg gaatggcgat gagataggtt
    25801 aataaaatgg aaattatact caacaaaaca gtccagcgta tagcttccca aataaccgtt
    25861 tttacgggct tttcatcttg ataagatatg ccgaagtcac ctaccataaa tttggtaatc
    25921 cattggtgat attgattttt tgtgccatac cattgtatgg ctggtataaa ttttttatat
    25981 gtagttgcat gttgtgtaac atcagcatac aattttgatg aattgacaat atcagtttct
    26041 aacactttaa gattgtcagc aacgtgcaac tgtgctagca tgtctttaaa taacaaattg
    26101 atagttttat cttcaggctc gatatataat tgttgtacgt cgcttttcat tgctataagc
    26161 gcatcagcat ttacagaatc gcttgttaca tttccgagtg actcttcgag ttttttgagt
    26221 gacaaataat aagcattgat ttgtttccaa ttgccatatt gctcaataag tttagacaaa
    26281 ttttctcgat gaaatttttt aggaatcgta tataaatctt ttggcgttgc actattagta
    26341 atagaaaagt agaaagtcgg caaatctagt cccagttgtt tgcgcttatc gatataagct
    26401 ttttctgcgg cttgagcttt tgcagagctg cccatttcgc tgttggagtt tagcatttgt
    26461 tctactggat cgccaggcac gttagtactc aagaaaaacg tcaacaacga aatcatgaat
    26521 aaggtaggaa tgaatagtaa tattcgttta aatatgtatt tcagcatact gtatattctt
    26581 aaaattagtt ttctacttta tagcctcttt ttactttaaa accagcaaac cagacgcctg
    26641 gataggcaga tggacctgag ttaatatttt caaatctatt gtgaatacaa tttttatatt
    26701 tttgcacgta taagtatatg taaggaattt gttcgtgttc aatttcctgc catttatagt
    26761 acaattcttt ccttttgttc acatcgaatt ctaccctgat ttcatcgagg attttatcgg
    26821 ttttcgcatt tccaaaactc atatagttgg agccaccatt agctgctgat gtgtgaaaca
    26881 attgcttatt atcatctaat ccagggctgg ttacccaacc ttggtaccaa agttggcatt
    26941 tgtgcttttt caattcatct aaatacaatg accactccaa ggatttaata gtaaattcga
    27001 tgcctacttg ctggaacgtg cgctgcatca acaagcccac catttctctc aagctattgc
    27061 cactattata gttatatgta atttctaagt tggttttttt gccttcaatc actttatcta
    27121 aaatgccatc accatcgcta tctttccaac cagcttctgt tagtaaggat tttgctttgt
    27181 tgatgtcgaa cggaaatggt tttatcgcag gattgataat atcttttttc atcggcaaaa
    27241 tagcgctatt ggtgcgtatg cctttgttgt aaattacttt ttctatgatt tgatcaacat
    27301 ctatcagata acatagcgcc tgacgaactt tcacatcgct tagtattttg tctttatgat
    27361 ttataccaat agcttgatat gctaactggt caggttctgc tttataaaag ttctttttga
    27421 attttggaga attatctaag tcgatataat ctttaactgg cgtaacgaaa ataaaatcta
    27481 gtttttcatt ttttaatgca gttaatgcag ttgtgaaatt attaattgtt acaaatacca
    27541 atcgctttgg ataagcttca aagaaaatat tttctttatt gattttgtcg ccccaccaat
    27601 ttttctttct ttctaatgtt agctgctgac ctgtttccca actcttcagc acataagcgc
    27661 cagagccttg cacaaaagct gggtcgcgca aggtttgttg tgagttaaaa aaatcagcaa
    27721 attcttttac atttttatca tctgcacatt ttggattttc aaccgtcagc tcgcttatag
    27781 tatatttttt taacaagcct ttagcgtcat aaacatattc tggcaatatg accacttcat
    27841 aaccagtgcc ttccactgct ccaatatttt tttcacagat aacagtatat tttctaggat
    27901 tatctggata ggttttaata tccacaacat aatcaaccga ggatttgaca ttatctgaat
    27961 ttacttttgg gcaaagaatc gatttgtatg aaaacaacac atcatctact gtcaccgatt
    28021 tgccgttgtc ccaagttgct tctggtctaa tctcataagt aatttccatt aaatcgccta
    28081 cttctttaac ttccggcata ctcttagcca aaacaggctt taataccata gttttcaagt
    28141 cgaagtacaa catagattgc ataatgtttg gcattacata tcttcttgac gtagcatcag
    28201 aaattgtaac tggatttaaa ccttgcgcat cggaaagtgt atgtatgact agttcatctt
    28261 tatatgtttt agaatcgttt ccactgttgc taaggactgc actgaacaca acaatcagta
    28321 agaaaacaac taatgtagta taaagtatat ttttaattga catattgcaa ttgaattatc
    28381 ttacaaatgt aagagatttt agagacaatt gtatggaatt aattcgtaat aaatgaatta
    28441 tagatggcat agacgcatga gaattttctt gtgtcttgcg tattatataa aatatcaaat
    28501 ctcaataaat acaagctcct taagaataaa taacagttca atacttttat tacattttat
    28561 tttcttattt tcatgcatac aatttccaac ctatgtacaa gttaattaat ctatcattat
    28621 tggtgatatt tttcgccttt ctacaagcat gttcaaatag tggtaaaaaa gctggcgaca
    28681 ctgatggctt cgtttacgat gaaaaaattg aaaaagaatt tcaagaaaag ctgattctgg
    28741 tagaagatgg tggtacggta gaattccctg aaggcaaatt cttatttaag aaagacttat
    28801 caatggaagg caagaaaaat gtaaccataa agggagcagg tgccggcaaa actattttat
    28861 catttcttgg acaaacggaa ggtgcagaag gtattaatat caccaattgc caaaatgtga
    28921 ccattgaaca attcactatt caagatgcaa aaggcgataa tcttaagctt aaaggttgtg
    28981 atggtgttat cattagacac atgaactcta cttggacaac tggcgccgac acgtctaatg
    29041 gtaattatgg ctattatcca gttgagtgca aaaatgtatt gatggaacat tgcgaagtgt
    29101 cttactgtgc agatgcaggt gtgtatgttg gacaatctac taatgttatc gtacgacact
    29161 gttatgcaca tcataatgta gctggaattg aaattgaaaa ctgtattaat tctgacgtat
    29221 ataaaaataa agcagaaaat aatgcgggtg gaattttggt tttcgatttg ccaaaattat
    29281 ttcaaggaaa tggcagaaat gctaaaatct gggacaatga ttgtttgaac aacaacttta
    29341 aaaactttgg aaaacctggc agtattgttg catcgatacc tccaggttct ggtttaatta
    29401 ttatggcaac agatagcgtt gatgtattca acaatagaat cattaacaat aaaactgttg
    29461 gcatatctac tatcagctat caccttacgg gcaagcccat aaatgacact acgtatggcc
    29521 cttattgtac tgccatttat atacatgata ataagtttga cagaagtgga ttgtccttaa
    29581 cagaaaaagt gcctgactta tctacagatt taggcaaact attgacagca acattcaaag
    29641 gtgcggtaga tattttagta gatggctcct cggatccaaa aatcagagat aaaaacggac
    29701 aattgcccga aggaaaaaga atatgtgtaa gaaataatgg caaggtcaca tttggcaatc
    29761 ttaacgcatg gaaagcaagt ggcatcaaag acatcatgaa ttacaaagat gatgacatca
    29821 caaaatttga ttgcagttac acaggtatta gaggcaataa cacagatcca gcaagcgcaa
    29881 aataaataaa catgaaaaaa attaccctat tcagcgtact tagtatttct ataagctgct
    29941 tatttctatt gaatcaatgt aaaacttcga gcaataaagt aaaccttgat ataaacgcca
    30001 aaccactgga gaagctttct gaatatcatt tctttacagg cgcacttaaa gatttgaatc
    30061 caaatgataa agttttgcct tatgacatca ataccgcatt atttacagac tatgcacaca
    30121 aagctcgttt tgtatggatg ccagaaggca caagtgcaaa gtataacaaa gaggaagcat
    30181 tagattttcc tgtaggcaca gttttaataa aaaatttctt ttatgaaaac gatgaaacga
    30241 atgcctcaaa aggaagacgt attgtcgaaa cacgattatt aattaaacgt gccgacaaat
    30301 gggacgctaa tgcgtatgtt tggaatgacg aacaaacgga agcattctta aacattgcag
    30361 gagacagcaa aaaagtagat ttcgttaatt tacatggtaa aaatatacat gttgattatt
    30421 ccgttcctaa taaaaaccaa tgtaaaaact gtcataactt aaacaatgta ttattaccta
    30481 tcgggccgaa ggttcgatat ctgaataagg attttaaata tgctgatggt tcaaaaaatc
    30541 aattggaaaa atgggcagca gttggttatt taaaaggcta taataaagaa gaaaacttga
    30601 caaataaaat ggcagatgct tttgatgtga aaagtggcac cttagaagaa agagcaaaat
    30661 catatcttga aattaattgc gctcactgcc accgtccaga aggtagtgct aatacttcag
    30721 gtttgttttt attaacgaaa gacaataatc cagaaagttg gggcattatg aaaagcccag
    30781 tagctgctgg tgcagcaagt ggagacaatc tatttgatgt tgttccaggc aaaccagatg
    30841 aaagcatttt ggtgtatcga ttatccgaaa atgatccaga aatacgtatg cctgaattag
    30901 gcagaagcat tcaacatgta gaaggtgttg cactcatcag agaatggata agtgaaatga
    30961 aataaaacat acctaatatt gttacaaaaa atgtcattcc actttcgtgg gaatcaaaat
    31021 attatttatg agattccatg tttcaagaga atgacgcttg ttttttattt tcttattaac
    31081 aaagaaaata aagttctatc tttatttcca tgaagcattt atttatttta gtactatgtg
    31141 taagcgtatt ttcgtgcaag caaaacaaag aaaacaatac attcgttgta gatgaaaagt
    31201 tacagaaaga gttacaagag caatttattc ttgtagaaga tggaggcact attgaactac
    31261 cggaaggcaa ttacaaattt agcaaatcgc tttctatgga aggcaaaaaa aacattacca
    31321 taaaaggaaa aggtgtcgat aaaactattt tttcatttct tggacaaatc gaaggcgctg
    31381 aaggcatgaa ctttagcaat tgcaatgcca ttacacttga aaattttacc atacaagatg
    31441 ctaaaggcga caatatcaaa ttaaaaggct gtacagatgt agtgattcgc cacatgaact
    31501 ctacatggac aactggtgcc gactcagcga atggaaacta tggatattat cctgttgagt
    31561 gtagaaatgt attgatagaa aattcagaag tttcctactg tgcagatgca ggtgtgtatg
    31621 ttggtcaatc ctataatgta acggtgcgca actgcgttgc gcaccacaat gtgtgcggta
    31681 ttgaaattga aaactgtatc aatgtagatg tccacgataa tctatcgcac aataatactg
    31741 gcggttttgt aatatatgac ttacccggaa ttccgatgaa gaatggtaga aattgccgtg
    31801 tatataacaa catagtacga gaaaataatt ttaagaattt tgcgaagcca ggcagctatg
    31861 ttgccgatat tccacctggc agtggcatta ttgttatggc atatgatagt gtagaagtat
    31921 tcaataacac attcgaaaac aataaaacat taggctgttg tgcggtcagc tactatgcaa
    31981 caggcaggcc gtttacagac agtatttata caccttattg tacttctata tacatacatg
    32041 ataatatttt caaacgcaaa accatgttga ttcctgactt aagcacaaat ttaggaaagc
    32101 tgattgtagc catgtctaaa ggaccggcag acatcattta cgatggcaat tattccaaag
    32161 agatgagtaa tactgacggt acattacagt cgcaatacaa atcctgtttc cgcaacaacg
    32221 gcgatattaa attctttaat ttaaacgcgt ataaatcacc agtaatagaa aatgtattac
    32281 acacctacga caacaacctc gctacatttg attgtagttt gacgaatatt agtggaaatg
    32341 ggaagtaaga atttaaacac ataatattcg ttacagcata agttatctta aaaaataata
    32401 gataaataat tgcatttgta aaatttttat gttatattta aataactaaa tccattttat
    32461 gaaaataaaa caagcattcg caagcattcg caagcattcg caagcattcg cgcatagtgc
    32521 gtatggcgag aaaccacacc gttctcttta gctttatgtt aatagcaatg gcattaatgc
    32581 tacaatcaca aactggtggt tgttgtagta ccccaaaccc accgtcacaa acacctactg
    32641 ccactggcac actttaccta ccaaggttac aaccaccaaa tccactttgg gataatccaa
    32701 gttgtaacag cactccattt tatcaacaaa cattacctaa tggtacaaca atgtatcctc
    32761 tagatgtaga tattgcttcg caaattacat tttattttaa agtaagagtt accactatag
    32821 atggttgtta tatacaaaat acttacttaa ataagaataa cagagtagca ctaagtggca
    32881 taggcataaa aataaactat ccaactacta agccttatgt tgttgaagtt tgggcgcaag
    32941 cagatggtac attttttagt tgttccaaca caatccctgg tgcaagccca gaatggtatt
    33001 gtaaatttgc aaataacgag aatgggcaac aaatatttgg tgaaggaaca tcagataatc
    33061 catataatct aacatttcat ccatttgtag ttgcaaatat tgtttttgca cataataatg
    33121 gtggaaatgg ttctggagct gttgaaccta aatttaaata caatggcaat gttccatttg
    33181 gtactaatgt tttaaactaa aaattaataa atttatgaaa acaacaacta tattattctc
    33241 gttcttatta ataggaatga caatagcctg taaaaaggaa ataaaaaaca ctactgatac
    33301 tagtaaattt agctataccg atgacaacat tacaccagca gaaatagcag caaactttat
    33361 ggcaagagca gatggtggta gttttactgt tggtgcaaat ggtcttgaag gtggatataa
    33421 tggtgaaagt attgatataa atgcaaagag aacctctgta gatataaatg gtagaaaatt
    33481 tactccagct gctaatacta tttggttagg tcgttattat gtacctgact atcttgcaaa
    33541 cactgaacaa gagataaatg aaatctatag ttactttaca aatgtactta ggggaccaaa
    33601 aactaatttt acactttatg atacacaaaa caaaaaatta gttggtttta ctatacgtaa
    33661 ctctatacca ataactttaa ctattgataa tcaaaattat agagaagcac cagctggtag
    33721 ccatattgta ctgcattgga atgctgactc agaaaataat ttaggtgtag ctattgcatt
    33781 aagtggagac aaaggattac gttatataca tgcagatgaa acaggaacat tagatattac
    33841 tgatgaagca ttagaaattg ctggaaacga tagaatttct gtaacattaa cgcgtggcgt
    33901 aattatgctt ggcaaaggtg ctgatggacg tgattataaa ctgacaaact gtataagtaa
    33961 aactgaaatc gtttatattt aatgaaacag atagtttcac tacgttcgca atgacgtata
    34021 caataaatcc acgatgcaag tcatggattt tttatatttg tatatgcaat acaaaagaat
    34081 gtccatagaa tcagaatcgc ctgaagaaat gggctatgcg tcgatacaat ataatttagc
    34141 agaaagttct gttcgcgata tttatttccg tgatttacaa atcaacttag acgaagtatt
    34201 tctttgttat gcggaacatc gaggaaatat tgcattgaga aatgagattg ttaaaaatga
    34261 aaaggaccta agcgccgatg atgtcttagt ttgtccaagt gcggcaactg ctttatttat
    34321 tatttctact actttattaa acgataagga ccatctggtg gtattgcgac caaattatgc
    34381 tactaacata gaaacaccaa gagctatcaa ttgtgagatt tcatttattg atttacaatt
    34441 tgaaagtggt tttcaattag atattgaaca aataaaaaat gcagtaaaac caaacactaa
    34501 attaatcagc cttacttcgc cgcacaatcc gacaggtgta gtttatgaca ataaaatatt
    34561 ggaagaaatt atagcatttg ctacagaaag aaatatcaac gtattggttg atgaaacata
    34621 ccgttacttg aatttccagc gtgatatgat gccttattat gctgctaaaa gtgacaaggt
    34681 aatttctgtt tgttctttat caaaagcaca tggcacacca ggcattcgaa ctggctggct
    34741 gattactaaa aacaaaaaat tacagcacga ttttctcgca gcaaaagaac aaattatcat
    34801 ctgcaattct gttgtcgatg aagccattgc actgcatata ttacaacaag aaaattatct
    34861 tcaaaaaacg cacgagcata ttagaaaaaa ctttagtgta cttaaaaatt ggatggacat
    34921 tgaacaacat tatttagaat ggatacgacc aaatgctggt gttgtatgtt ttccaagaat
    34981 taaaaaagaa tatactgttg acaccaagaa gttttataca atattatacg aacatttcaa
    35041 gacttttgtg ggcgcgggtc attggtttga acaagcagac cattatttcc gattaggatt
    35101 tggctatcca actgaagaag aactacaaca aggtttaaaa aatatagacg cttcaataga
    35161 agcgtctata gtatcgtctt agaagctatt tattagttgg ctgctgtcga tggcatggtc
    35221 atttctttga actctacatc acttggcaat gcgaatgttt ctgccgaaaa gcttggattt
    35281 tcatctaatc ttttcaaggt cgtcgttact gattttccga ctacggacat ttccatttta
    35341 ataggaactt gtttccacat ccatattttg cctttcattt gttctgaatt aaagctcgac
    35401 actctgcatt cttttccatc tactgtttct gtgccttcat ctttataatt cagttctttt
    35461 ctcaattttt cattcatgct tgaaaaatca agattattag ggtcgaattt agactcatct
    35521 atcttaaatt tcatgcctga tttattgcct gtttgccagc tatacacata gccatcaagc
    35581 cttaaactat tggtcgttgt gttcatactt ctgccaccaa aactaatcgc agcaacgact
    35641 accgtatgcg atttttttcc ataatcatca aacgaaacag tagatgatgt cgtgcccatt
    35701 ccgccaggca ttactgtttc cgtctggtaa attgccgctt ttaggccttc atttgtgatt
    35761 gcctctttga cctcgctgat tacattttct gtcgtagatt tttctgctgt tgtcgtgtta
    35821 tttttacaag ctgtaaaaat tagtacggta cataatgcta aaagtagttg tttcattaat
    35881 gttgttttat agttttaaaa attatttatg tctatctgtt taaagttgtc accaatggca
    35941 tcaagtaaaa aacgtattta aaagtaagca aaaaattgat gctccgtgtg atttagtatg
    36001 taatttgatt tcttacagta aaaataatca ctcacaaaat gatgatactg tgtatttaga
    36061 tgtgacaatt atctattttt gtagaaggaa ttttttaata catgcattgg cacgaactca
    36121 gtacggaaac taaagaacaa ttagaacaac ttaagctgca acaaatggca aaacttagtg
    36181 tagatgaaaa agtgatttgc gtaacgcgat tagaagatgg tttttttggc atgcacaata
    36241 cgtgtccaca cgctggcgca caattacacc acggacattg caataaaaaa ggaattatca
    36301 gttgtccgct gcatggctat aaattcgata tcagaacagg aaaaagtgca gatggcaata
    36361 actatgtcgt gcggaaatat caatttaaaa tagaagacaa taaattgtgg attggttgga
    36421 aaaattatta actcaatttt gtcctttatc atttgtctat gaaatataca tttacagaaa
    36481 taaataacat tgtaaacggc agcattttgc agtttgacga agatacgatg ctcacgctcg
    36541 cctgcatcga cacacggcaa attactaatg cagcgactag tttgtttttt tgtttaatag
    36601 gtagaaacaa tggccatcaa tacattgata ttgcttatga aaaaggcata cgcaacttta
    36661 ttgtttctga agaaatagat tttacaaaat atgctgctgc gaatttttgc agagtggaaa
    36721 acactaatgc ggcattacaa caattggcca tttatcatcg acaacaattt actattccta
    36781 taattggcat cactggtagt aatggaaaaa caatcgtgaa agaatggctg tttcaattat
    36841 tatctacaga aaaaaatatt gtaaagagtc caaaaagtta caattcacaa attggcgttc
    36901 ctttatctgt tttactgatt gaatcgacgc atgagcttgc catttttgag gctggcattt
    36961 ccacgagtgg cgagatggac aagctgagaa acatcatacg gccaagcatt ggaatattaa
    37021 caaacattgg ctctgcgcat gatgcaggat ttaatgatag aaatgaaaaa ctggcagaaa
    37081 agctaaaact gtttgaaaat tgccaacagc ttatttactg tgataaatat gtagatgaat
    37141 cgcaattatc aaatcttaat tgccaacaaa tctcatttgg tgagcaagaa aaatctatct
    37201 atagaatcat acatacagag aagaaaaata gttccacaat cattgatatc atttcgcaaa
    37261 gaaaggaaaa agagaagtgg caatttataa ttccatttag cgatgatgct tctgtagaaa
    37321 attgtctgca ttgcattatt gtacttttgc aattaggatt tagtgcagct gttattcaaa
    37381 caaaaatttc gatgttgcgc aatctgccga tgcgactaga attaaagtat ggcatcaata
    37441 attgcagtat tattgacgat tcatatagtg ccgattttca ttctttgcag atagcattag
    37501 atttttatcg acaacaagac actcataaaa aaagaacgct tattatttca gagtttatag
    37561 attctggttt atcagaaaag gaatttatat taagattaaa atctatttta caacatacaa
    37621 aatttgataa aataattggt gttggtaaag catttattca cgaaatcgaa tcattaacag
    37681 gcgtagttaa agaatggtat gtttttgaaa gcacagaaaa actgatcgca caatttaatg
    37741 tgttgcattt cgacaatgaa ttggttttat tgaaaggcgc tcgtctattt agttttgaaa
    37801 aaattacagc gcaacttatt ggacaaacac atgctacagt gttagaagtc aacctaaatg
    37861 cgctttcaaa taacttacaa gtttataaaa gttatatacc aaaagcgtgt ggtgtaattg
    37921 ccatggtgaa agcattttct tatggcagtg gcagcattga agtagcgcga ttattagaga
    37981 aagaaaaaat agattatttg gcagtcgctt acgctgatga aggtgtggaa ctacgtaaaa
    38041 acaatataca atcgccaatt atggtgatga atcctgatgc agtagatttt gatagaatgt
    38101 tggaatatca tttagaacct gaaatatatt ctatatctat tttgcagcag ctcattgact
    38161 gtattggaaa tagaaaaatc gatatacata tcaaagtaga aaccggtatg aatcgtttag
    38221 gttttgaggc cagcgcaatt tctgaattaa tcaagctttt gcatcaacat caaaacatta
    38281 gcgtaaaaac tattttctcg catttggctg ccagcgaaga tgcacatttt gacaacttca
    38341 caaagcaaca aattgcacaa tattcacaaa tagccaatac cttttgcgct gcctttaatt
    38401 atcctattaa aaagcatctt gcaaattcga gtggcatctt gcgatttccg gaagcacatt
    38461 tcgattttgt tcgtttaggc ataggtttat atggtgtgga tagtgcttcc aacatacaag
    38521 acaagctaca agttatcggc aaactaaaaa cacgtatagc acaaataaaa catgtgcaag
    38581 caggcgaaac ggttggatat tcacgcaaag gtgtagtgca aaaagcgatg aaaattgcag
    38641 tgctcgctat cggctatgcc gatggttatg acaggcgctt tggcaatggt attggcgaag
    38701 tttttattgc tggccagcgc gctaagatta ttggcaatgt ttgtatggat atgtgtatgg
    38761 ccgacatcac gcacatcgat gatgtgaaag atggcgacga gtgtgaaatc tatggaaagg
    38821 aaatttcgat tattgaacaa gccaataaaa taggcactat ttcttatgag ttgctgacgc
    38881 gcatttcttc gagagtaaaa cgtttgtatt atctagatta aaaaaaatcc gtctaacgac
    38941 ggatttttga atttcgtaat ttttgaaact ttattaactt gccaattcgc ttgtatttgt
    39001 tggagtttcc gcttctactg cacttgatac tgctactatt tcttccttgt catcgtgatt
    39061 gatttcattt gcagtcgttt cacttattgg tgcttgtatt tccgcttgat ttattgcagt
    39121 ttcttctgct ttgttctgtg ctttctgctc ttcttcttgt tgacgtttta gcaatttttc
    39181 cgctcgctct tgttcttgtt tcttttcttt ttcgatgtca ttttttaatt gcgtaatcgt
    39241 ttgcaaaatg cctgcagctt tctcttcttt ttcttttgtt ttgtctaaca aatagccaaa
    39301 tctgtttttc aattcatcag cagagagttt accacgcgtg tagtgttgca ttttttcaac
    39361 ttgttggttg tagctctcca tatctcgtgc gatagaatct ttaagaccgt gcaaaatctt
    39421 attgagttgc gaaattctgc cttgcgtttt cgaaatgact tctgtcattc tgtatttttt
    39481 caaattatca aaaatgtcat ttatttggtt ataaatggct cgtttatgat tccatttgat
    39541 tgcagtatct ttcagttcag cttgtatttt tttaagttca tctgcaatgt gtttccattg
    39601 gtcgttgtag atgagttttg cttgtaaatc ttctaatcgt tttttataat tcttgacaag
    39661 cacattattt gcttggtctt cattttcgtg gttaatgcgt tttactgctt ttaatgcatc
    39721 aaataccgca tttattttat cttttagtgc tgtagtttgt tcccagctga tttcattttt
    39781 cttttgatac gatgcaattt tttcccattg ggtttttgct ttgtcccata atgtttggtc
    39841 atattctgct actaccgaag ctttttcttc gagtaatttc acttcatctt ctaatagctg
    39901 attgattttg gaactcatca attttttgct ccacttatgt tgtgattgca acacaacatc
    39961 taagcgttct actttaactt cttctggcaa aatagaatcg ctgttagcat ctatttccca
    40021 ttggattgca ccttctggaa aggtgtattc gccaccattt ttccattcta caaataaagc
    40081 gtcttgaatt tctttagtca ccatttcctt tggaaatgca tacgtgtgga tttttgatgt
    40141 ttcttcatca agctggatag cgattaatgc ttttctgtct gtgccaatct cgccccataa
    40201 aactaatcgt gtgttcatgt ttagtcgtca attgtaagtc atacatcgta aaaatatgct
    40261 gaattaacct tgcctatttg tacgagaatc tttttgtaaa ttaataatat ttaataaaaa
    40321 aaacagtttg caaaatacat gcaaactgtt gtacaaaaat aagattttta tttaattttt
    40381 caatgatgaa cgcttggatt taacagtgtc gatgatttgt tgctgcaaag aaatttgttg
    40441 ttgttttgcg gcttgatcat ttgtatttct tacgatatcg ctttcccaag ttgcgatttt
    40501 tgctttagct ttttcaattt cttttagtgt tttttcttta ttttttttca aagattttaa
    40561 ttctttttct aatgttttta gtgttttttc ttctgatttt aattcatcgc caacggctga
    40621 taaagcttgc gactgtgcaa aatctttgat aacagcatcc atgccagcat attgtgtgcc
    40681 gtaggctgcc gatgtaataa atgcgccacc taagtcagta aaaaaagtaa cctttgaacc
    40741 attttctatt ggtgtgacaa tggcataaat atctaccgta tttgcgctta ccgtattaat
    40801 agcagcattg tcaataaatg tttcaggcga ttttttactg gaagtaattt tagctttata
    40861 tttctttgct aagcttttta ggccttcttt tgctttgttt acactagagc ctttcaagat
    40921 aatttcgatg ccgttttgct cgcctttgct cataaattga cgcacttcat tggtgacaaa
    40981 tgtgttttgt gcctgcgcag aaatagtgat tagcaaagca atgatgaatg caagtgttgt
    41041 ttgtttgtac gctgtcatga tatactgaaa ttaatttaaa gttgctcgtt tttgtttaac
    41101 ggtttcaata atttgttgtt gcaaagaaat ttgttgttgt tttgcttttt gatcagcatc
    41161 atttttaaga atttcttgtt cacgcttttg aatgagctct tttgcttttt caatatcttt
    41221 taaatagcca tctttatctt ttcctaaatc ttttaaatcg ccatttagtg atttaagtat
    41281 tttttcttct gctttttgct ggtcttctac taccacgata gcttggtctt tagcaaattt
    41341 tttcaaggct gcatctaagc ctgcatactg tgtgccataa gccgctgatg acacaaatgc
    41401 gcctcctaag tccgtataga tggttaattt agagccgttt tcaattggtg tcacaatggc
    41461 atagatatct aatgtattcg cacttaccgt aggcatttgc gcattgtcga taaaaatttc
    41521 tgggtttttc ttgtctgtaa caatttttgc tttcattttt ttaccccatt tttcaactgc
    41581 atctttagca tcgccaggct tggtgccgtt taaattaatt tcgataccat tttgctcgcc
    41641 tttactcata aattggcgta cttcgtttgt tgtaaattga gattgtgcaa atacactcgt
    41701 tgcagaggca agtaatactg ctgcaactgc tgttgtttta ataaaattct tcatattggt
    41761 tgtatttatt atttaacaat acctgttatt ttgtttttat tatactgcaa aatgaatgcc
    41821 acaaaatagc cttgttcgcg aacgtatgtt aacacttctt ttgctttttc taaattatca
    41881 aaatctccaa gtctgtagat aaatctatta taggcaatca cttcttctac tttgcctaga
    41941 tgtgcaaatt ctggaaattg aatattgcct tcatcataac ttgctaattg aactttataa
    42001 taattttcag caattggtgc tgcttgttct ggttttgcaa ttagtatgtc tgttttggca
    42061 gcaggaatat ttggcatttc tgctaccatt gtattatcat tttttggtaa atcatttgta
    42121 tttgtttgtg ccgcaactgg tgtttgcaat tttgcttgtg gctttgtttc caccattgtc
    42181 ggattaatgc ttgttattgt cgatgatgat tgctcaattt ttatttcgtc tggaacaact
    42241 ttctcgattt ctgtgtcatt atttttttga tgtagtactt ttattttttt agatttttca
    42301 ttatccacca tatctgctgc taatgcaata gattcatttt cgacttttaa tggctggcta
    42361 atttttgcaa tcgtatcaac tgtaattgtt ttcgttataa ttatattttg tgaacttact
    42421 ttatcttgtt tgtcggaatt aatattttca acatgaagaa catcagtgcc tttgttaact
    42481 tcggaacttg cttctatttt agttcctttc ttttcttcta atatattctt tttgggtgtt
    42541 gatgatgttt cttgttgtac aagttttgca ctcgctcctt tatttgtatt gattggattc
    42601 tgttcagcta ttacacgtat taaagagtct attttttctt gacaaatagg ctcatgaatt
    42661 atcgttgtat ttgccacttc aattttttgt tcttgcgtta aaacattgct gagcttaatg
    42721 tcgcttggca aagtagctat tgtatatact ttattctcaa gcaatttaag tttagagcca
    42781 cttaataaat aatctctgac tttgatgctg tttcttgcag tgcctaatgt tgttaactca
    42841 tctgttactt tattatcagg tgattcaaaa attactttat aattattgtt caataataat
    42901 tttatttcaa atctgcctgt gctgtctata tctacttcgc gtaatttctg tgtggtataa
    42961 ttcaccatac gcattttgat gtttttcgca ggtttgtttg tagccgcgct aattactttt
    43021 ccagtaaata caatataatt atctaatgat attgtatttg acgcaccttt tgtactacta
    43081 aattggctat ctggcttgag tggcacatca ataaatattg aatcaccatt agctttgcca
    43141 aatgaattca cttttatcaa tgcaggacga taagcatctt tcgatacttt cagcgtatag
    43201 tttttgtttt tatttatttg gaaagcagaa atgccatttt cgttagtaac gccttcaaat
    43261 aatttatcac cagcatctat tacctgaacg agtgcatagt ctattggttt ttccgaaaaa
    43321 gtatctttcg cggcaactat caattgcaag ttaaatggtg tatatctata cacgtcaaaa
    43381 ttgcctttac caccacttct attcgatgta aagtagcctg tttttgtttc attatcatag
    43441 ataatgctat tgtcgtcgcc tgccgaattg attggtgctg gctgcaacac ggcgccttcc
    43501 caaattgctg ctgtttttat cacactataa atatcaaaac cgccaaaacc accttctcta
    43561 tttgatgaat aatacaatat gttatcggca ccatcttgtg gcataaatgg attgatttca
    43621 tcagcagatg agttgaccaa tttgcctaaa ttttttggtg ttgaccaagc attatttttt
    43681 agtgttgttt gccaaatatc gtagccgcca aatccacctg gcatattcga tgaaaaataa
    43741 atggtagtgc catttttatt aaaagatgga tttttaagcg aatatccatc aacatcgata
    43801 ggaagcagtt ttacgtttaa aaagttgcca cctaaattat cggctatata aattttttcc
    43861 agttttgatt tatgcttttt tgatgctgac ttctcatcta tagctgaaaa aacaacattg
    43921 ttaccatctt gtgtataaga caaacctgta atcgtcatat tcggcacatt gttcatatat
    43981 gcttttactg gtggtgcaaa attttcgtaa gcacgcacaa tctgctgtat ttcatttgta
    44041 gttctatctt ctggagtaat attatttttt gttgttatat acacaggatt ggtgcgcaac
    44101 acggcgatgt atgcttcgtt gtcagctgtg ttgtaagtat agttttctaa tttataatta
    44161 taagcaagtg ctgaagagcg tattactctt tcacattgat tagcaagatc cattacttta
    44221 tttacatcac cagtcaaagc agcatacatt aaataagcgt ctagggcttt ctgatattcg
    44281 cccatcaatc gattggttgt agcgtaatta aataaattaa ttttagaaga atacaccaat
    44341 tggctttctt tatcatacca tgacaatgca gttgcatact cttttttctt tacataaagt
    44401 tcagcaatac ggaatgcatc ttgtggatcg cgtttatcgc tatcatatct tgttttataa
    44461 aactcgataa ctttatctat ttcgcctttg gcaacgagct tactgatata agatgcgtcc
    44521 catttttttg cgtttgcaaa aattgtgatc agcatacaac taataaaaaa gtgtttcttc
    44581 atattgttca attaatgtgc ttccagccaa ttatcaccat agccagttga tacttctatc
    44641 ggcacttgta aaggtaatgc attttgcatt tcacgaataa cgatgtgttg tatttcttgt
    44701 ttttcaggca gaaacacatc aaataataat tcatcatgca cttgcaatat catcttagat
    44761 tgaaatttgt gtgcttttag ttgcttgtga atattaatca ttgcaatttt tatcatatct
    44821 gctgcgctgc cttggatagg cgcattgatt gcattgcgct ctgcaaaacc tctgatagta
    44881 aaatttccag cgttgatatc gcgtaaataa cgtcttctgc cgagtaatgt ttcaacatat
    44941 ccattttttc tggcattatg aatcgtgctg tccatataat ttttgatgcc agcatacttc
    45001 tcaaaatatt gttcaattaa ttgtgctgct tctgtacgtg gtatgcctaa tcgctgtgct
    45061 aagccaaaag cagaaatacc ataaatgatg ccgaagttaa ccatcttagc ttggcgacgc
    45121 atttctgatg ttactgcttc caaaggcaca ttaaacacgt tggctgccgt tgctgcatgt
    45181 atgtccaaat ttttagcaaa agcatctatc atgtttgcat cttcgctgat tgaggcaacg
    45241 atacgcaatt ctatttgtga ataatctgct gagagcagta catgattttc gtctttatga
    45301 ataaatgctt gtctgatttt tctgccacgt tccgttcgaa ttgggatgtt ttgtaagttt
    45361 ggattaacag aacttaacct gccagttgct gctatggctt ggttgaaggt agtatgtatg
    45421 cggtttgtct ttgcattaag caaacttggc aaagcatcta cataagttga ttttagtttt
    45481 gtcagctctc gatagtcaag caattgagct gctattggat gttcacttgc tattttttgc
    45541 aaggtttctt catctgtaga atactgacct gtttttgttt ttttgcctgg atatttaatg
    45601 cccatttttt cgaacaatat atcgccgagt tgttttggag aatcgatatt gaactgcaca
    45661 ccacaaatgt tcaatatttc atctttcaat tttacaatat ctgcacctat ttcgttggaa
    45721 tatgctttca aaaatggaat atcaattttt acaccttcga attccatatc agtcaataca
    45781 ggaatcaatg gtgtttctac ttcataaaat aatttatgta gatgtgaatt ttcaatttct
    45841 tttttaaata cgttatgtaa ttgcaatgta atatcagcat cttctgctgc atattcttta
    45901 atcttttcca aaggcacatc acgcatagtg ccttgttttg ggcctttctt tccgattaat
    45961 tcttcaatag aaaccggtgt atagcctaaa taattttctg caagcacatt catgttatgc
    46021 ttcgtgtccg catctattaa ataatgtgcg agcatcgtat caaacaatac gccttttacc
    46081 tcgatgtcat accatttcaa taccaattgg tcgtacttaa tattttgtcc gattttaata
    46141 atcttatcgt tttccaatat tggtttaatg tgttgcaatt gtgcgattgc tgatgtccta
    46201 tcagcgtcga taggaatata ataagcttct gttggtttta tgctaaatga tatacctacc
    46261 aattcgcaat tattggcgtc aacgtttgtt gtttctgtat cgaaacaaat atgtgttgct
    46321 gtatttaatt gctgcattaa tgcttgcagc tgcgcatcat tttctattaa agtataatga
    46381 tgtgtggtgt tattgatgtt ttttccaaat gaggtagctt gcaatttttg tgttggtggc
    46441 attgtagaaa tcgcatctaa ttcttcgctt gcaaacattg aagtttgtgc tgtgtttttt
    46501 ggcgtttctg tttttggatt ggtgatgttt tttatgctat cattagcaga ataatcatct
    46561 ccaatcattt tttttcctaa tgttctgaat tcgagttcag aaaaaagttg agcgagtttt
    46621 tctttattaa aagctttgag ttgaaaatca ttttcattcc aatcaattgg cgcatcagta
    46681 atgatggtag ctaatttttt tgaaatcaat gccatttctt tatgttcgat gagtttctct
    46741 ttcatggcac cttttacctc atcgatattt tcgtaaatat tttcgataga accgaattgt
    46801 cgaatcaaca ttttggcacc tttttcgcca atccctttca caccaggaat attatctaca
    46861 gcatcgcccc acaatccaag aatatcgata atttgctttg gctctttgat ttcccatttt
    46921 tttatcaact cttgcacacc tacgatttct gctggctttc ccataaatgg tggcttgtgc
    46981 aaaaagatat tatcggccac aacttgccct aaatctttgt cgtgagaaac aatataacac
    47041 tcaaaccctt gagctgctgc ttttacggct agtgtgccaa ttaaatcatc tgcttcatag
    47101 ccggctattt ctacaactgg catattcata gcttttacaa tttccttgat gtaaggaata
    47161 gaagcgacaa tatcgtctgg tgtttcttgt cgatttgctt tataaaattc atgttccgct
    47221 tgtcggtcag tttgagcagc agcatcaaat acaatagcat aatgtgaagg attgtatttt
    47281 gtttttattt cccaaagtgt gtttaaaaag ccttgtattg ctgacacatt aaagcctttg
    47341 gagtttatca aaggtgcttt ttgaaaagca aaataggcac gaaagatgag tgcgaaagaa
    47401 tcgaatagat agagtttgtt catcgttaaa tatgagtata tgcgttaatg aatttatgcg
    47461 tttatgatca agtattaaat cttacacgca tgaattactt tgtaatgcct gccagtattc
    47521 tgctgctctg cgagtgtgag gaatgcaaat actaccacca actaagttag caattgcaaa
    47581 cacttcatac acttgtgctg ttgaaatgcc agcttcatac gattttccaa gatgatattt
    47641 tatacagtcg tcgcaacgca acaccataga tgccaccaag ccgagcattt cttttgtttt
    47701 tgcatcgatt gcaccttctg cataggcatg catatctaaa ttaaagaacc gattgagtgt
    47761 taaattttgt tgtgctaaaa tcacttcgtt cattttagca cgatagtcat taaattcttt
    47821 tactgctttt tccataaaat aaaaaaacta ttatctgtct aaagtacgac aaataatagt
    47881 ttaaatgttc agctaaaaag aaattactta ttttttcttc ttcgctgctt tgcgattttg
    47941 tgtccactct tcgaacaagt cgataccgcg atgcattgct tctaaacgtt catagtttag
    48001 tgagtaataa atctttttgc catctcttct tgtgcgaact acatctacac ctcttagaat
    48061 ttttaggtgt tgtgatgtga tagactgttc aattttaagt gtgttgtaaa ttacgtttac
    48121 attcacttct tttttttcat ggatcagctt aatgattttt aatcttaagt gatgagccac
    48181 ggctctcaaa agctctgctg ctttctcgat tttctcggtg ttaaattcaa aattttccat
    48241 aaaatctttt tttatgattt agttcactac aaatatatag aattatttgt aataaaatat
    48301 aaaatatttt gaggtttctc aaaatattta atttaaacaa attatggata gtaatgttta
    48361 taaaagtaga atattacttt actagttctg agagtaatct cgaaatttcg tcgagacgtt
    48421 cgtggtcaac agaatagtag atatattttc cgtcgcggct ggtgacaact acacctgcac
    48481 gtctaagcaa cgctaaatgt tgtgatgcta ctgactgctc tatacgtagt ttaatgtata
    48541 agtctgtgac agtcattttg ccatttttat ctattagttg tataatttcc tgacgaagct
    48601 tatggttgac agaacgcagc actaaaacag atttacgcaa aatttgtaag tcaattttta
    48661 gttcaggttg tacatggtta ttattattta ctgtcataga tttcatttat gtattttttc
    48721 tttatccgaa tattcaaatc ttataccaat tttttataag aaataaattt tggggaaaag
    48781 gttgcattgg ttataatata ttttccataa aatctacata aattaatgct ttgaaactgt
    48841 tgtatataca gcctttgtgt aatttggctc gaaataagct atatctacaa attgttgcgt
    48901 agtatagtgc ttgtaggcaa gcgcacatac atcagcagcg ctcggcgtat attctgtata
    48961 gacaacattt gtaggtataa tatttgccag tggcacttta aatgcgccat ttccaaagaa
    49021 aatatatttt ttcgttttat cgagtagtgt attaaattca tcatcaataa taagcgatga
    49081 ataggcttga acaagctgtc catctgcttt aaaaaaagac gtaaaaactt ccattcttcg
    49141 tgcatctatc atgggcacat agacatcata agctaattga tggtaacggt gttgtatgcc
    49201 aaacaacatc gcctgaaaag tggaaatgct gattatagga atttgcaatg caaaagcata
    49261 gcctttagca gcagcagcag caatgcgcaa gccagtgtaa gaacctggtc ctgagcttaa
    49321 agctatggca tgaatgtcat taaaagtgag cgcgcacgat agcaacaatt cttcaataag
    49381 cttatgcagt gtgtcggcgg ctttatttga tgtattgatt tctcttacag caaagcaaat
    49441 atcatttttt gcgatagcaa ctgaggtaac agcattggcc gtttcgatac ataagatata
    49501 aatagggtgc tcgtttgtat gcattatttt tgagcagaaa gcatggattt atatttcgct
    49561 acgtcagaca aaacttcaat ggcttttttg atttctacat catctttcaa actattggca
    49621 attcttcctt tttcataggt atatctgctt actatttctt gttctaataa atgttttatt
    49681 tcgtctttat atttgattaa atctttcgat ttatcgtgtt ttatttttga ttctaagtct
    49741 gtaattactt gctgtactgt ttctgagtaa ctatcttctt tgattgtttt tttcaaatca
    49801 tttaacttca cttcgcttgg tgttgtatat tcataatctt tgccttttag ataatcagtg
    49861 aactgcgtat attcttcatc agacaaggag aaattatttc catctcttaa tgtttgatga
    49921 tcatatttat attgatttgc aaaatggaaa aagtgattat tggtaaaaag tgcaatagtg
    49981 actggtgcta ctttttcttc ttctgttttg atatctggtt caatgcctgc accatcttta
    50041 acgatgcgtc ctgattttgt tttataaatc ttacgtaaag aatcaggcaa tgcacttgcg
    50101 atgccatctg cacttctgtg cgaatagtcg atacgttgta tgcatctgcc actaggtata
    50161 taatattttg aaatggtcac ttttaatttg gccttgtatg ccactggttt tgttacttgc
    50221 accaagcctt tgccataggt attttgtcca ataataaggc cacgatctaa atcttgaatc
    50281 gaacctgata cgatttctga tgctgaagct gaaccagcat cggtcaacac tgccaatgga
    50341 atatcgatgt ccacaggttt tcctgttgtt gaatactgaa aattggcttc ttgtattttt
    50401 ccttttgtat tcacgatgtt tacgcctctt tctacaaagg tgttgcaaat attaatggct
    50461 tcattcaaca agccaccacc attgccacgc acatctaaga ttacgccctt aatgtttgga
    50521 ttttttttca agctacgcaa tgcatcttgt acttcttgtg ctgcattatc agtgaaggaa
    50581 atcaatttga tgtagccgat tttatcattg accatgccat agtaaggcac gttttcaatt
    50641 tttatttctt gacggttgaa ggtgtatgtt ttttcttcgt tggaaattgc tttttttacc
    50701 ttcaccacaa tttttgttcc cggttctcct tttaataatt ggctgacttc gtcagagttg
    50761 tattttgtcg ccactttacc atcgatttct acaattaaat cgcctgcttg caagcctgct
    50821 ttttgagcag gaaagccttc ataaggatcc gtaataataa caccatcgcc aatcttagaa
    50881 atacgagcgc caatgccacc atatttgcct gtcgtttgaa attgatattc atctaagtcg
    50941 gctcctgtga tgtaatttgt aaatggatct aagccgtcga gcattttatt aatgccatct
    51001 gtcattaatt tatttggatc tacgtcatca acatagtagg tatttagctc tttatacaat
    51061 tgtgtaaaca attccatgtt tttagcaatt tcaaagtagc gattttggtc tgctgcagta
    51121 gcatttaaca acaagcaaag acaaaatatg gtgatagcgt ttttcatatt cttttattta
    51181 gcgtatgcat ttatttcaat gatattaaac cttaaagcta ctaaaaaatt acagtaacaa
    51241 tggttaaaaa atacaaagag aattagggta caggatcgta gcctgagccg ccccaagggt
    51301 ggcaggataa aatccgtttt attgccagat atccgccttt aaataatcca tgtttttgca
    51361 aagcttctat ggcataagta gaacaggatg gttgataacg acatttattt tcacctagga
    51421 gtggagaaat agaatattta tagattttta tcaatgcgat aaatggaaat atgagaattt
    51481 gtttgagttt cattgttaag ataaatataa taataaatta tcttacattg tcaatttgtc
    51541 acaaattatt ctacttaatt tcttttgctt ctttactgac tatttctaaa cattctataa
    51601 gtattgtata tgatttgaat acaatcggta atatttcaaa aaaggtattt caattgctaa
    51661 gttgataaga aattataggt gcttcttata cgtgatgatt attgttgcat ttatggcttg
    51721 aacttagttg acaataaaat tatcttggct gattgatttt ttcatcataa aaaaaaagag
    51781 gttttggtag ttctgtttga tttactcttt ctctattaaa catttcaata cattcatcat
    51841 acattttata tcttttgagc tttgggtcta atctatcaaa caatgttttc aactttttga
    51901 tatcatactc tatagtatcg tagcctagat cttcaaaggt atattttagt gcagaataaa
    51961 tatattcaag cgtaagaaat gaacttaaat gtgtttcgta aaataattta acatgtttgt
    52021 ctgatagttc attgcataaa ggcaaatatt tttgtaataa tagatgcgca ctgtctctat
    52081 ccataccatt gtatgggtag agaattgcat gcataatatt gtatttttta aacatactat
    52141 cgtttatgcg catcatttct ttgtattcat catttaaaga agaacccacc acagtcgcag
    52201 tattattttt acaatctata tttaatatgt atttgccttc atgtaaataa aattgaaaag
    52261 ctttttctgc atcattattg atatacaatg tagtatgaat cggtgcgtct acataataac
    52321 aaatggaatc tattgtacct tccattcttg atgtataata aaaagtagtt tcgctgctta
    52381 tataaaaaaa agtatttgtg tctgtattcg acagatttaa ttttattcta cagttacttt
    52441 catttgcatt tatatttgaa atacaaatca ttagaaagta agctaatatt attctattca
    52501 tagattaata aaatctatct ttttatattc taattaacaa gga
//
LOCUS       DAOQQV010000005        44156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_231_length_44156_cov_7.352940, whole genome shotgun sequence.
ACCESSION   DAOQQV010000005 DAOQQV010000000
VERSION     DAOQQV010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 44156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44156
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231_length_44156_cov_7.352940"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="rocD"
                     /locus_tag="PLL59_01150"
     CDS             complement(<1..795)
                     /gene="rocD"
                     /locus_tag="PLL59_01150"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HMY22435.1"
                     /translation="MNPLHSQQLIDLELKYGAHNYHPLPVVLSKGKGVHVWDVEGKQY
                     LDFLSAYSAVNQGHCHPRIIKVMQEQAAVLTLTSRAFHNNLLGVYAEYITTLLGYDKV
                     LPMNTGVEAAETAFKLARRWGYDVKGIEKNKAIIIVCENNFWGRSVAAISSSTDPSSY
                     GGFGPYVPGIVKIPYNDIAALEAALQDKNVAAFMAEPIQGEAGVVVPDEAYLSNVKAL
                     CSKHNVLFIADEVQTGIARTGKMMACDYESVKPDILVLGKALSGGTM"
     gene            1083..2804
                     /locus_tag="PLL59_01155"
     CDS             1083..2804
                     /locus_tag="PLL59_01155"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMY22436.1"
                     /translation="MKINYILSFPAPHTHYVEVEMHISDIANNNLQLKMAVWTPGSYL
                     IREFQKNIDFIEYKAENNLWKRLEKSDKNTWLLNVENRNEITLRYAVYSFEYSVRTNF
                     VDTTHALINGASTFLYIDGAEDTALNINIHPFDEWQQISTALPQAGDNKWLRTAANID
                     ELIDSPIEIGNHTSYFFQAASIQHELAIYGESNIDIEKIIEDLKKIIEEEVKIFGSHP
                     CEKYVFIIHHTDSSYGGLEHLNSSVNHITRWSYDKENYQRSISLLAHEYFHLWNVKRI
                     RPSTLIPYNYNSENYTDLLWFFEGITSYYDDYVCYRAGVTPLDSFLKIIEKNINDVIN
                     NTGFNVQTLSESSFDTWLKYYRRNENSINAHVNYYTQGALITMLIDLSIIHASNGQKS
                     LDDVMRTLYDAYLKQNYKGITEEDIILAIDSVCPINSKNLFDQYLRSTTPMHVIDFFE
                     NIGIEANEKSDENVIYLGLGFSWKENKLMITQVDKQYGASIGGLSAEDEIIAIDGFRV
                     NKNYKNIYAHKKVNDTIEVTIARQGILKTLQVPLTADRFKKITLQKLSNPNESQQIKL
                     EKWLGKN"
     gene            2995..4362
                     /locus_tag="PLL59_01160"
     CDS             2995..4362
                     /locus_tag="PLL59_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22437.1"
                     /translation="MKKSKLLIIMLFACNGVFGQSFLGGHFSTREALLSNTLNPAAGI
                     AGEMKWQVNFLGLSSEVGNNYFSINGSLKGIANNWDKEKYIGQNLDGKRKELHANIGL
                     VGPSGFVRIKQNNAIHFGIRARAVLTVNDINQDFVYSMYNHFEEILQWLPNFNDERAT
                     AAANVYSEIYAGYARTIKIGERHAVHLGLTAKMNSNIFNAQFVANDIDFNKIVTSPTD
                     SFINVGNSKFDLFVSDVIDDGFKYKFGANGFGVDLDIIYEYKLKNSNEHFVMAGISLN
                     DFGFNTYKLGKNSRSFVGNGRDIPASHLTDAFGETNNIDEVLDSLGTRTVPTGTKKIK
                     TPATVNVFADVRVVKMFYINANMQFNPYSFKKGTAVANLPIDITVTPRFEHRIASVYL
                     PINYNQYSGFNMGAGFRIGQFTLGSSTIITSFAKKKFTGVDFYMNIGFGASEKIKKSK
                     KKEKA"
     gene            4533..4712
                     /locus_tag="PLL59_01165"
     CDS             4533..4712
                     /locus_tag="PLL59_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22438.1"
                     /translation="MKGVFILIAVILLSIFTTDSFAKCKKYKKSKSSSNIYVSKKYKG
                     HSCPKPRKIINAVYF"
     gene            complement(4709..5494)
                     /gene="trpC"
                     /locus_tag="PLL59_01170"
     CDS             complement(4709..5494)
                     /gene="trpC"
                     /locus_tag="PLL59_01170"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HMY22439.1"
                     /translation="MNILDKIVAHKKNEIVQAKSLTSIKQLEESIFFERPTLSLSQKL
                     IQENATGIIAEYKRKSPSKGIINDKYTTLDIVKQYEAAGVSGSSILTDNLFFGGTVQD
                     LISIRNAVNIPLLRKDFMIDEYQFYEAKSIGADVVLLIAAILTPQEVKNFTTIAHSLN
                     LEVLLELHDESELQHVCADVDMVGINNRNLKTFEVDIEQSISMSEKLGNSFVKVAESG
                     ISSVATLKQFKQHGFQGFLIGENFMKTENPGFACQQFITELLA"
     gene            complement(5533..6522)
                     /gene="trpD"
                     /locus_tag="PLL59_01175"
     CDS             complement(5533..6522)
                     /gene="trpD"
                     /locus_tag="PLL59_01175"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HMY22440.1"
                     /translation="MKETLQYLIDGNVLTAQEAYSVMKDIASGKQNEIQIAAFLTIFN
                     MRLPTAEELNGFSKAMLELATPIKFKSNNVLDIVGTGGDGKNTFNISTLACFVCAGAG
                     VKVAKHGNYSVSSVSGSSNVLEHAGISFAKNQSELNEQLEAANITFLHAPLFHPAMKN
                     VANVRKQLQVKTIFNILGPLVNPAKPKVQVIGVYSDSIAKLYREVLKLRDVNFAIVHA
                     LDGYDEISLTADTKILYRKNIQVSSAHDLGFKNSKAAELFGGTTVSQNAKHFINILKG
                     NGTEAQNNVVIANAALGISLYEQNSYSAAIQQARHSLLSGSAYDCFLKLKSFK"
     gene            complement(6588..7151)
                     /locus_tag="PLL59_01180"
     CDS             complement(6588..7151)
                     /locus_tag="PLL59_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HMY22441.1"
                     /translation="MKDILVLDNYDSFTYNLVHYIESFNVKCDVFRNDKITLADVDKY
                     DKILLSPGPGIPEEAGVLLDLIKAYAPTKSILGVCLGHQAIGQVFGAELYNVGQVIHG
                     KPKNTKVIAHDILFDGLDIEFQSGRYHSWAIKNVISPLKVTAIDDDGIVQAICHETYN
                     VRGVQFHPESVMTPQGKIIIENWLFKC"
     gene            complement(7188..8591)
                     /locus_tag="PLL59_01185"
     CDS             complement(7188..8591)
                     /locus_tag="PLL59_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HMY22442.1"
                     /translation="MKHNFKTSHKKIIADMLTPVSIYLRLRAKYKNCILLESSDYHGD
                     EHNFSYICFQPIAGFKVESDKFIINLPDRNSEIISLKENKLCDLISDFISKFSNFSYS
                     ELPIITNGLFGYMTYDVVQYFEDIQLNKPAPAIPIAQYFCYQYIIAFNHFNQELFIIK
                     NEIEQVTNDTSTLDDIESFIKYKPLQLDTFACDAEKTSNLNDAEYLEMVNKGKQSCYR
                     GDVFQIVLSRQFQQNYRGDDFNVYRALRSVNPSPYCFYFDLESFRLMGSSPESQFIIK
                     NNKVTIHPIAGTFRRTGDDVKDAALAKKLADDPKENAEHIMLVDLARNDLSRGGKNTT
                     VEVYREVQYYSHVIHLVSKVTSEIDENANKIKMIGDTFPAGTLSGAPKYKAMQLIDNY
                     EKTSRSFYGGCIGHLNFNGDFNHAIMIRSFLSKDNTLTFQAGAGVVADSVPESELQEV
                     ENKLAALNKAIALANTL"
     gene            complement(8866..9996)
                     /gene="hisB"
                     /locus_tag="PLL59_01190"
     CDS             complement(8866..9996)
                     /gene="hisB"
                     /locus_tag="PLL59_01190"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HMY22443.1"
                     /translation="MQKILFIDRDGTLIEEPKDNFQVDSFDKLKFLPQVNTYLKKIVD
                     ETDYKLVMVTNQDGLGTKSFPEKTFWPVHNLMLSVFETEGIIFDDIFIDKTFAKDNAP
                     TRKPNTGLLTKYLDASKYDLKNSFVIGDRLTDIQLAKNIGAKGILIGRSADKTDDKKL
                     NNKELNTFIALKTNNWKSIYELLTTPQRIATIQRNTNETKIAIKLNLDGNGKSNIKTG
                     LGFFDHMLEQLSKHANCDIDIKVKGDLHIDEHHTIEDTAIALGEAFLQALGNKKGIAR
                     YGFLLPMDDVLAQTAIDFSGRPWLVWKVKFNREKVGDMPTEMFFHFWKSFADAAKCNL
                     NIKAEGDNEHHKIEAIFKSVAKSIKMAVHRDGNELPSTKGML"
     gene            complement(10005..11066)
                     /gene="hisC"
                     /locus_tag="PLL59_01195"
     CDS             complement(10005..11066)
                     /gene="hisC"
                     /locus_tag="PLL59_01195"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMY22444.1"
                     /translation="MKNSTIDNLIRPHIVKMKPYSSARAEYTGDDGIFLDANENPIGS
                     VCNELHNRYPDPLQQNLKNALASIENLKANHIFIGNGSDECIDVLIRTFCESKEDAVV
                     VCPPVFSMYEHAAHAQNIQVHEVLLTENFQLNIEALLPLISKENNIKIIFICSPNNPT
                     GNLIKENDIEIILNHFKGIVVIDEAYQDFSEKVSWTNRLSEFHNLVVIKTFSKAYGMA
                     DARIGMLYADDAIIHYMNTIKMPYNVNQYSQTLALTALHHIDKKNEFVATLNAGRKYI
                     QDELAKTSFIQQVFASDANYVLFKVENANDLYKYLIANKIIVRNRDSAPLLKGCLRVS
                     VGTRAENEKFIEVLRNYIV"
     gene            complement(11056..12339)
                     /gene="hisD"
                     /locus_tag="PLL59_01200"
     CDS             complement(11056..12339)
                     /gene="hisD"
                     /locus_tag="PLL59_01200"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HMY22445.1"
                     /translation="MQVIKYPSKETWQQLSQRPTQSLEEIEKVVSPILDDIKLRGDAA
                     LFEYAEKFDKARISNLLVTEEEIATAIATVDVGLKNAIDIAYKNILYFHQQQQTSAEI
                     IETSAGVFCWRKSLPIDKVGVYIPGGTAPLFSTVLMLAIPAKIAGCSEVVLCSPPNQD
                     GTINPVILYAANLCGVHKIFKVGGAQAIAAMAYGTQTIPQVYKIFGPGNQYVTAAKQL
                     VNKSGTAIDMPAGPSEVLVIGDDESRPDFIAADLLAQAEHGIDSQVIFVTTEEMYLNL
                     VHFAIDEQLKILARKSIAEKALENSKMILLKNLDDAIAFSNVYAPEHLILQTRNAEEL
                     ANQVSNAGSVFIGHYTPESVGDYASGTNHTLPTNGFAKAYSGVSLDSFQKKITFQKME
                     KAGLENIGPAVELMAENEGLTAHKNAVTIRLKNEK"
     gene            complement(12391..13248)
                     /gene="hisG"
                     /locus_tag="PLL59_01205"
     CDS             complement(12391..13248)
                     /gene="hisG"
                     /locus_tag="PLL59_01205"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HMY22446.1"
                     /translation="MQKLKLAIQKKGRLSDESIKLINECDISFPTSSGKLVTAAYDFP
                     IELLFLRDDDIPDYVQDGVADIGIVGENVLVESDKKVDEVKKMGFGKCRLSLAVPRTM
                     NFNSANDLSGKSIATSYPKILQNYLDKNNIHAEIHIISGSVEIAPSIGLADAICDIVS
                     TGSTLLSNGLKEVEQIFQSEAVLIKNKSLSTEKQEILQQLLFRLEAVKRAQKNKYILL
                     NAPNESLDKIISLLPGISSPTVQALAKEGWSSVHSVITEHDFWEKIQQLKEAGAEGIL
                     VMPIEKLIY"
     gene            13539..15266
                     /locus_tag="PLL59_01210"
     CDS             13539..15266
                     /locus_tag="PLL59_01210"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HMY22447.1"
                     /translation="MKKILLIANLLIYISLSSFAMQNDVLNFDNYKEEFTKRLWKQYP
                     DWATGIGYHEYDNVLFVPNALQRTKEKAFFEQELSKLHSFSLSTLTDANKIDYKILED
                     FFHYNIFLIDTYKSWQWNPAEYNVGASFSYILSENYEPLSKRLNSFYQKLKNVPAYYT
                     AAKLNIQHPTKEHAQLAIDQNEGTLTIFEKDFIDSVKTMHLKPAVEEAYLKRAEAAAK
                     SIRSYLHYLKNYKNETPRSFRIGSDLYAERFKYEIQSKYTVDEIYLAAESRKEYLHDE
                     MEKLAIKLWPKYFGDKPQVKDKLQLIKMVIDTISDQHVEPALFKQEIEKQLPELAAFV
                     EKKNLIYLDPSKPLKVRNEPGYMAGVAGASMSSPGPYEKNGTSYYNVGTLDGWSAERS
                     ESYLREYNKYVLQILNIHEAIPGHYVQLVYSNKSPSIIKSILGNGAMIEGWAVYSELM
                     MMENGYGNNEDEMWLMYYKWNLRTVCNTILDISVHTKDMSKEAALDLLINQAFQQQAE
                     AEGKWKRVQVTSVQLTSYFTGFYEILQLREAYKLQKGAQYSVKEFNEKILSYGSAPVK
                     YIAEMMLKK"
     gene            15449..16636
                     /locus_tag="PLL59_01215"
     CDS             15449..16636
                     /locus_tag="PLL59_01215"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HMY22448.1"
                     /translation="MKPIFILFFSVIIFCFSCKKENSNDRKIPIPDQTEINKLKINQL
                     QILGSHNSYHKHMDNRLFAFLGNINFLLPEKYKVDALDYMHESIPDQLNKYGMRSFEI
                     DIYNDPNGGRFYNRNGNRFVHMTVESHIADLQQPGLKILHIPDIDYETHYYTFKKMLT
                     ALNEWSDAHPNHLPLFILVETKEETVGDVLGFLGFQQAIKYTPALASAIDDEIKSVFG
                     ADLKKIITPDQIRGTYATLEEAVLAKNWPTVGASRGKFIFVMQGGAEDEYLVAHPSLQ
                     GRAMFIMSEPGKPEAAFIGYDNPIEKQAETIQAVQAGYMVRTRSDEPNVQNKNGSYEQ
                     QHAAFSSGAQMISTDYYRPDTRHASSPRMFTNYSCNFPNNELARINPISAADKQNIGI
                     IAE"
     gene            16660..17796
                     /locus_tag="PLL59_01220"
     CDS             16660..17796
                     /locus_tag="PLL59_01220"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HMY22449.1"
                     /translation="MRIIFYVFILLTANGIAQENKIIEQLKINQIQTIGSHNSYHLRA
                     NKFVLRFLKGLAAILPKEYNPKDLDYAHEPLFTQLDSFHLRSFEIDVYADPNGGHFYK
                     RKKNNLLGKPKISKITALKEPGFKVMHIPDIDYNTHYFTFKSMLSDFKKWSDSHPNHL
                     PIYIMIECKEETIADKIKSFHFTKAIQFTPALCDDLDNEVKEIFGESLQKVITPDEVR
                     DTFATLRDAVLAVNFPTLDKARGKFIFIVMQQRDTYTKNHFSLKDRAMFVFSDANAAE
                     AAFIKYDDALHDENEIKKAVQIGFIVRTRADGPNIQNRSGDYSQQDAAFRSGAQIIST
                     DYYRPDIRHQKKPRKFTNYATRFIGNDIARINPINSTNYTIEEK"
     gene            complement(17801..19036)
                     /locus_tag="PLL59_01225"
     CDS             complement(17801..19036)
                     /locus_tag="PLL59_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY22450.1"
                     /translation="MRRFFTLLFFCISLSAFSQIRYKDEMFSISKHTDVMYGRNYDNK
                     NQLTDLLMDVYEPQSDTAALRPIIFFVHGGSFVGGDRQDQSIDEIAEFFAKKGYVTAN
                     IEYRVQQTTLISPFVDFADMYNWYRAITRASHDLKAAIRYVKKDVATNYNQYKVDTNS
                     IFIYGSSAGAITALHTVFLDDTLEMNPQFRVAFREMGGLDGNSGNLGYSMKGIKAIVS
                     CSGAIADLNYMNNNRDIEYLGFHNNPDLTVPFDVGCFVTVACWLGNFYGANKIFPKIR
                     NNGTYAEFYPVNRLGHPVDQVSDTVARPMILQKTTDFLYRIMNQQIVTSIRNNVVQSF
                     ELFPNPSKGNFTIQLPRTLQYKAVRVVITDVVGKNVFSVLVENKENIDINVDLANGIY
                     VVSIIADEQQYLSKLTIQQ"
     gene            complement(19233..20540)
                     /gene="thrC"
                     /locus_tag="PLL59_01230"
     CDS             complement(19233..20540)
                     /gene="thrC"
                     /locus_tag="PLL59_01230"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HMY22451.1"
                     /translation="MKLYSTKHISPDVDLKEAVLKGLPSDNGLYMPYEINPLPQKFWD
                     NLDNYTFCEMAFHVAKHLIGDAVPDEALNKIIQDAYNFDAPLIEVGDYSVLELFDGPT
                     LAFKDFGARFMGRLMGYFLKDNDKEVNILVATSGDTGSAVAHGFLNVEGIHVTILYPA
                     GKVSDIQEKQFTTLGANITAIEIDGTFDDCQALVKQAFLDKELNEKLTLSSANSINIA
                     RLIPQSFYYFYAYKQLDDKSLPLYFSVPSGNFGNLTAGLFAKKMGLPITKMIAANNSN
                     DIFTVYHETGVFEPKRSVQTLSNAMDVGNPSNFARMMDIFANDVQKVREEIAAYSFNN
                     EQTASCISNIFEEYNYIMCPHTAVGYLGMEEFVLDNDLNPYYINGVILSTAHPVKFGE
                     LVAPLIGEEVAIPERLQSIINGKKLSIPMSANFDVFKAWLMSK"
     gene            complement(20647..21594)
                     /locus_tag="PLL59_01235"
     CDS             complement(20647..21594)
                     /locus_tag="PLL59_01235"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HMY22452.1"
                     /translation="MKVGDKIRVFAPATVANVACGFDVLGFAIDKPGDELIMEITAEK
                     GVVIEEIEGDDGVLPRDAKKNSATVAIQDYLDFIEADFGCKIWLKKMMPSGSGLGSSA
                     ASAVAGVFAVNMLCNEKLTKQELMPFLLHAEKAACDAAIADNVAASLFGGFILVRSYE
                     PLDILQIPVPEDLWCAVINPHVIVLTKDAREILPKNIPLAHSLRQSANVGGMMVGLLR
                     GDYDLIGRSLVDYIAEPYRSKLIPGFYEMKNAAKNAGALGGSISGSGPSVFALCHGKE
                     IAYKVGEAMKNVMNEMNIGADVYVSKVNNQGPKVLPLID"
     gene            complement(21655..24120)
                     /gene="thrA"
                     /locus_tag="PLL59_01240"
     CDS             complement(21655..24120)
                     /gene="thrA"
                     /locus_tag="PLL59_01240"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HMY22453.1"
                     /translation="MKVLKFGGSSVGSTDNIKRIIEIVRAELDKNEEVVLVCSAFSKV
                     TDQLINAGKLAEKKDNKTYLEIFESFKERHRLAIAELVSDEEIKAVMMEEFVESMLRV
                     EDILNGINLLGELSDKTLANLVSYGERFSSFIVAGALRSAGLNGIYVNATKLIRTDSN
                     YLSAKVNFEKTNKNILDFFEAVNGVAVVTGFIGCNEHGDVTTLGRGGSDFTAAIFGAA
                     LKADAIEIWTDVDGVLTADPRRVEQAFTIPTLSYKEAMELSHFGAKVIYPPSIQPAYI
                     KNIPLVIKNTFNPTHPGTFVSKDSGKSNNDIKGISSISDIAVLRMEGTGMVGVVGIAS
                     RLFGCLSRAGINIIFLTQGSSEHSICFGIVPNEIKKAQKSVEEEFRYEIQNRQIAPLV
                     VETNNSIIAVIGENMSNVPGVSAKMFKALGKNGINVNAIAQGSSELNITAVIDKHNEA
                     KALNALHEIFFESDVHSVNIFLVGPTGLIGKTLLKQIHNQFEYLKKEKSMQINVTGII
                     NSNKMLIDSNGIDLSNWETTLVEKGEKSDLDNFTNKIIDLNFANSVFVDCSASKNVVE
                     FYEKLLKKSVSIVTPNKIANTLSQDKYVRLRELAKKSNAQFMYETNVGAGLPVIGVLH
                     SLLNSGDKILKIEAVLSGTLSFIFNTYKGDLKFSDVVLDAKAKGFTEPDPRDDLSGLD
                     VARKALILSRDSGAQMELSDIKVENLVPENLREVDVKTFLDKLPELDALYESMKKDAE
                     SKGNVLRYMAVIENGNAAIELKQVDAQHPFYNLSGSDNMIVFTTERYKNNPLVIKGPG
                     AGAEVTAAGVFAEIITIGNYMAD"
     gene            complement(24441..25469)
                     /locus_tag="PLL59_01245"
     CDS             complement(24441..25469)
                     /locus_tag="PLL59_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMY22454.1"
                     /translation="MKNIILGALLLILAYQSRSQDINSSQFYALPSNVNPAFTGFFNN
                     DYYVAAAYKNQWSSVSAPFQTVGATGQVSLLKNKRPNSIVGVGVDIIHDRAGSTSFNT
                     SLFNLNVSYLQVLDVKRQHIIGVGFQNSLVMRRFDLSKATFGNQFNGYDGFDQGINPG
                     ESGLNTKQLDYNLGIGGLYTYSPKEHNNVYVSFSAFNLTRPNVSFYDNTEHRLYTRFT
                     AFAGGEVSLKGSWSLLPSAIFQKQGPSMEIMVGSFARYGLVRNRKEMLGINFGVWYRV
                     NDAVVPAVKVEYKGFNITVNYDINVSKLTKVSRFNGSGEISISYSGLLFKEHKKPTKP
                     IFCPTAGF"
     gene            complement(25515..30011)
                     /locus_tag="PLL59_01250"
     CDS             complement(25515..30011)
                     /locus_tag="PLL59_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMY22455.1"
                     /translation="MSKDKGCAPLYVTFNNTSTGNQDSCFWDLGINGNTADECNPSAI
                     FNQPGTYNIKLTIYKGTQTSTITKVVTVFRDPISRFDAAPRTGCVPFNVQFSDLSTVG
                     DAPITSWLWDMGDGRTETVKNPLHTYTFSGNLTVSLIVTDANGCKNTETVRDFIKKAT
                     PPTVDFTVNKSQTCLLPFPAIFQSTVTSASPVTYTWNFGNGDASSIANPTYNYTNIGT
                     YNVSLTVKDQNNCTATKTIPSAVKIERFKVNASVPNPLCTDQAVTPSVNSTYNPIFCN
                     WNFGDGNISNIVFPNISYANTGNYTINLKATNLEGCRDSMTQNVTVNLSPKAAFSADV
                     LKSCMPYTVNLSNSSQNGSSYKWFISGPNGFFQTYTSASPSVNLPNRGSYDVKLEVTS
                     ASGCTDVLNMPQYLWIGPDQINATADKMEGCEDLKVFFNANLTHNWTPRSIVWDFGDG
                     TTGTGENPVHTYMNPGDYTAKVTVTYDAPCESLVDVVGPIHVGGKYPFNGTMDYDKVC
                     VNKEMVTYTATGGIPTTEFIWIYGDGTGQGRNTTHIYTEPSQPRKYNVQLIAINNTCR
                     DTLDVQEIFVAYPKAAFNYSSTCNSQKVDFVNLSKGHTAATWDFGDGTILSTLDKNLS
                     HTYAPGTTQVTASLVVRNDSTGCIDTLVKLIKFSNIDSFRFTTSTKMGCKPLTVTLTA
                     VEDTNIVAYLWDIGNGTLVFGNNYVAKYFNEGKFVVKMFVKYRNGCVMESTQKDTITV
                     IGVKPNFNFDKASGCVPAIINLKDTSQSRFSPLSNYTWNFHDGSTASGSEVSHTFNAL
                     GSFPVKLTVENQYGCKDSITKPVTISEVKADFDIDVNNVCGGKPIKFINQSSANATTF
                     LWDFGDGTTSTDTFPIHTYSQERNYTIKLTVTDGNGCSNTMVKPGFVKIKNIHVNFTA
                     SPTFKTCPDLITDFQLQNPNNLQLSSVQWDFGNGNTSNDNNYNPQGVYTRSDSFDVKL
                     IVTDNNNCVDTVFKPKYIIVSGPSGSFNFMPDTGCAPFNVTFDAQFKNTTTTIWDFGN
                     GDTREDRTLANQSTYTYRREGEFTPTLVLKDDYGCTVNIISTKKVNVARMVPYFGVDK
                     TSVCSGSGSVAINDSLYTSPNSPIKEFYWTFTDSTNIPTRGVSDTFIPVGMGSYKINF
                     FAENTFGCIVKDSVRVGVYSTPVIAAIDDKLICKGEEIALQVEGNPSRISWSPNRFLN
                     VNNSQNVIAKPDSSTQYIIKAYNYPQCPVYDTVNITVKTKLAARAYPDTNICIGDTVQ
                     LHALAENTSLNTTKITWMSSPTLTTNTLDPYAFPRTNTTYYALFENGKCQMQKLPVVV
                     NVKPLPTVTAGQDHIIIKGQEVQVDATSPNNVSYLWSPDYKLSCTQCPTPMASPEVDT
                     FYYVTAITEYGCKATDKLRIRVIEDCAGKMVYVPNTFTPNGDGQNDIFKVYGPGVASV
                     KELRIFNRWGQIVFETNDPENIGWFGTFNGQELNPGVFVYYMDVECINGERTIKKGDI
                     TLLR"
     gene            30294..30980
                     /locus_tag="PLL59_01255"
     CDS             30294..30980
                     /locus_tag="PLL59_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HMY22456.1"
                     /translation="MNGICHLSVVPMRKEAADASEMVNQLLFGETFDIIEKTEKWSKI
                     RIHQDKYEGWIDNKQYLEQEKKKNTNKHCVSNLLLNKKGIRYPLGSYVAFDTANNNND
                     IIKTAKLFLNTPYLWGGKTCLGIDCSGFTQVVFRVHQIQLLRDAYQQATQGKKIAFAN
                     CISGDLAFFKNANNRIIHVGIIIKKQNKLEIIHASGCVRIDTLDEQGIFNHTLERHTH
                     QLDCIRRVLK"
     gene            31000..31689
                     /locus_tag="PLL59_01260"
     CDS             31000..31689
                     /locus_tag="PLL59_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HMY22457.1"
                     /translation="MDKKNIVILSGAGISAESGIKTFRDGDGLWENHRIEEVATYDAW
                     LNNRDLVLEFYNQRRKQLLTCEPNDAHRQLVILEEKFNVQIITQNVDDLHERAGSKNV
                     LHLHGELLKVRSTADERLVYDWKIDLVNGDKCEKQSQLRPHIVWFGEPVPMMDAAAKL
                     AAKADIFIVIGTSLVVYPAAGLLHYTDYTVPKYVIDPNRPVVSNIQPIEYITEKASIG
                     VRKLVERLMVE"
     gene            complement(31692..31946)
                     /locus_tag="PLL59_01265"
     CDS             complement(31692..31946)
                     /locus_tag="PLL59_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22458.1"
                     /translation="MQDSIFLHFNIKDKNLQSYKIILFNQYTKKIWYKEEANVTSADF
                     NFSKKIFFELQADTTAYVNVLGVDKNGNTGVAGHAFYLKK"
     gene            32138..32623
                     /gene="cdd"
                     /locus_tag="PLL59_01270"
     CDS             32138..32623
                     /gene="cdd"
                     /locus_tag="PLL59_01270"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HMY22459.1"
                     /translation="MQNLVKIEINYICYTSWHEVPEKYQHLMEEAKKAKEGSYAPYSN
                     FNVGAALLLNNGKIIHGSNQENASYPIGFCAERTALSAAASLASHEKISAIAITCSSI
                     KNPVTKPAAPCGICRQTIFEAECKHHQNIEIILMGESGEIFHFKSIKDLLPLHFDADF
                     L"
     gene            complement(32633..33319)
                     /locus_tag="PLL59_01275"
     CDS             complement(32633..33319)
                     /locus_tag="PLL59_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22460.1"
                     /translation="MKKLLLPIALLLSFIIGIAAVKGGNPKVPPTPTKDPKAEKRKVD
                     SLQFVNFTIDSKDHTFQNTTNEVLLNNGMKVQIVKLTRFPIPRGVAVTIPDDISQRFA
                     YELAEVEIKATNTTSADVKLSAAASEALFNSLKFYAAETGSKAFLSQYPLSFGSIYPK
                     MEPAQPEKLTPVFKSTQAMINDTYKPGESKTSKGIIICIAKAAKTVDKIIVINQEFGT
                     NKSYACPAKL"
     gene            33400..34284
                     /gene="bla"
                     /locus_tag="PLL59_01280"
     CDS             33400..34284
                     /gene="bla"
                     /locus_tag="PLL59_01280"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HMY22461.1"
                     /translation="MPKIFTFLFLFFVTYLGFSQENEIIKQLDLLTQQAKGKLCVYAY
                     HFEDSASINYYGDEMCVQQSVFKFPIALAVLEKIDKGDFSLQHKIHITAKDMQQETWS
                     PLRDSFPNGNINVTFENLLKYMVSQSDNIACDVLIHHLGKAKSIEKFVRKFDIDDIKI
                     RYNEEKMQKNWKNQYKNIGTPKAYVQLLQQFYDGKIISKPSTDFLYKVMVETTTGKNR
                     IVNLLPENCIVAHKTGSSGTNKDGMTAATNDVGIITLPNGKHIGIAIFVQDAFAAYNI
                     IEKTIAETALLIVNHYNK"
     gene            complement(34303..35535)
                     /locus_tag="PLL59_01285"
     CDS             complement(34303..35535)
                     /locus_tag="PLL59_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HMY22462.1"
                     /translation="MSDNKIKCPNCNTSIDIDVVLNQQAEDKYKKAFDEKLQLANVEF
                     VKQKEALALQQKDFEDKKAKENELFQKRLDQKIEEEKVRISAEQGKKIKEEFEQQLKS
                     QTEELEKRKQENVALKQKELDFLKRENELKEKEQEIELQLQRKLNEERELLKEKIAKQ
                     EQEKNDLRFREYEKKLEQTQKALEEAQRKASQGSMQLQGEVQELALESLLKEAFPFDL
                     IEEVGKGSKGADCVQTVRNNFGQVCGKILFESKRTQAFGGDWIEKLKADMRAITADIA
                     VIVTQTMPKDMEQFGEKNGIWICTFSEVRALVHVLRSTILKVFAVSKSQENKGDKMQL
                     LYDYLTSNEFGEQWKAVREGFLAMRHSIDTERTQMEKLWKAREKQLDKILLNASHISG
                     SVEGIAGTENIDIKLLDE"
     gene            complement(35554..35832)
                     /locus_tag="PLL59_01290"
     CDS             complement(35554..35832)
                     /locus_tag="PLL59_01290"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HMY22463.1"
                     /translation="MSIETYTCAYLITINAISFIAYALDKLFAMTKSQRISERALLIL
                     ALVGGFVGACMSMIIFRHKIKDITFLPYLLLISLSWIIGIIVFLSIYF"
     gene            35924..37885
                     /locus_tag="PLL59_01295"
     CDS             35924..37885
                     /locus_tag="PLL59_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HMY22464.1"
                     /translation="MAEKQIHGLHKLSAAGVLISLGIIFGDIGTSPIYVLKAVMNMAT
                     GGNHKITEDLVLGAVSCVFWTLTIVVTIKYVILALNADNKGEGGIFALYAIVRRYKAK
                     WTIIPALIGCASLMADGFITPPISISSAVEGLTILYPGIQTVPIVITILAGIFLAQQF
                     GTNRIGSLFGPIMFVWFSMIGTLGFFQIIQNPSVIKALNPMYAINLVLNYPGGFWFLG
                     AVFLCSTGGEALYSDLGHCGKQNIRVSWTFVKIALVLSYLGQAAFIINHKDFDYENIA
                     PFYAVMPEWFLKIGIVIATMATIIASQALITGCFTLINEAMKLKLWPNQKIIYPSIVQ
                     GQIYFPFINWMLFLGCVAVVLIFRESGKMEAAYGLAISLNMIMTTSLLVYYMYVKRKP
                     KYFIYGFIAVYVWIEFTYLVANLSKFSHGGWFAVLIAVVIFVLMYLYNEGKKLRKKHI
                     EFVEIKDFLDDITDLQNDTTIPKEATNLVFMCNANDKKHIDSNIIYSIFRKKPKRADT
                     YWLVHVDITNEPYGASYSVDTIIPKKCFFIRLKFGFKVEHKVHVMFTKIVEDMESRGE
                     IDLMSHYPSLRKHGYPADFKYVIMNPLVSIDNKLNPFEQFLIKSYNWFKSISLSTQED
                     FGLEKTNCRVENVPIRIAQKTHIEIRRED"
     gene            complement(37910..38617)
                     /locus_tag="PLL59_01300"
     CDS             complement(37910..38617)
                     /locus_tag="PLL59_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22465.1"
                     /translation="MKYLKIARFIGVAIIITGVVLLFTNPKAENNLMPGFKTPIIAFE
                     FINSKEAVLQYFTVQNPEQYEQQMLLGNKIDYLFMCLYSSFIFCIAMFIKKSTSSKLM
                     NIAILMSVLMLLGDALENSQIHQLIIKRNIDLNAINPIDNYALYGVHLSLLHIFTWIK
                     WSAIACSFVVFATYFIKGNLFYKTIAMAAVSSFIIGIATFVNPGFSSELFSTNVLIVF
                     VMLIVFTFTNNLKPEIA"
     gene            complement(38744..39352)
                     /locus_tag="PLL59_01305"
     CDS             complement(38744..39352)
                     /locus_tag="PLL59_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HMY22466.1"
                     /translation="MKKIELEIINITQGFTHHHSYAVVLGEIKGKRRLPIVIGGTEAQ
                     AIAIAMENMPPARPLTHDLFKTVMDTFHVTLSEIIITNLLDGVFYSKLICIFNGEEYE
                     IDSRTSDAIALAIRFNCPIYIYGNILDNSGLILMEETNPEIDEIEDVILDAVSSVSEK
                     DKTDYTIYTYKELSKMLDEALANENYEKAATIRDELNKREKQ"
     gene            complement(39475..40443)
                     /locus_tag="PLL59_01310"
     CDS             complement(39475..40443)
                     /locus_tag="PLL59_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HMY22467.1"
                     /translation="MAILFLVEIADGKVKKASFEAASYATAYAKQTGKDAVGLALGTI
                     DDASLSALGIYGAKKIIHANNAGFDNFDASAYAKAVAEVATQVNADTIVISQTLTGKA
                     VAPVVSARLKAGIVSGAVGLPDADFVVKKAAFSGKAFAFVKVNTAVKVVAVVPNSFGA
                     TKGEGTATVEASTVAPAASRITVKEVTRQTGDVAPLPEAELVVSAGRGMKGPENWGII
                     EDLASALGATTACSRPVADVHWRPHHEHVGQTGVAIRPNLYIAIGISGAIQHLAGVNQ
                     SKCIVVINKDPEAPFFKAADYGIVGDAFEIVPKLTEAVKALKASQH"
     gene            complement(40446..41189)
                     /locus_tag="PLL59_01315"
     CDS             complement(40446..41189)
                     /locus_tag="PLL59_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HMY22468.1"
                     /translation="MKFLVCISLVPDTTTKISFVDNDTKFNNDKVQWILNPYDEWYAL
                     VRALELKEANGGTVTVVNVGPAENDQVIRKALAIGADDAIRIDVVGDADSFAIASEIA
                     AVAKSGAYDVVLLGKETINYNGSNLGGMLAELIDLPFVSYASKLDMAGNTATIEREIE
                     GGKEVLSVDTPFVISCAKGMAEARIPNMKGIMSAKTKPLAVVAPQGAASLTSVVKYEL
                     PPAKGACKMISADQPEELIRLLHEEAKMI"
     gene            41252..41578
                     /locus_tag="PLL59_01320"
     CDS             41252..41578
                     /locus_tag="PLL59_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22469.1"
                     /translation="MHTNRIHRLESMIVENPRDTFALFALAKEYEKTNNYIKSVELFE
                     RLLVIDSNYLGAYYHLAKTYEQIGEVKKALNIYETGISLAIEQNNTHTLSELRNAKMN
                     LEIELS"
     gene            41704..43056
                     /gene="tilS"
                     /locus_tag="PLL59_01325"
     CDS             41704..43056
                     /gene="tilS"
                     /locus_tag="PLL59_01325"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HMY22470.1"
                     /translation="MLLRIQENIIHNNLANTGTPILVACSGGIDSMVLCDVLLRLNFK
                     IAIAHCNFQLRGKESNADEKFVAQFAKQHQLEFHSIKFDTTNYKKNNSISTQMAAREL
                     RYNWFEQIRKENKYHSIATAHHLDDQVETLLLNITKGAGIKGLTGIPAKNGFIIRPLL
                     KISKQEILDYASLNKIAFREDSSNASDDYQRNLIRHQITPYLEKINPSFHNSIDEFIG
                     RMNDYQALTNQQIESIKKKCLREKNGIIEIKLGYIKAHFAGATVLFHLIKEFGFNIDV
                     VQNILKVKESGKQFFSETHRIIIDRKNLFIATKNPERDSIIILEKIPSQIIFNNYKIQ
                     CTSIPIQEVNIKTSNRYAYFDADKIELPLIIRYAKEADYFYPFGLSKPKTPGKAGKKK
                     LSKYFKDEKFTLLEKENTPIIFSGEKVIYLVKHRIDDRFKISEKTKTVLKMVIIDDSI
                     "
     gene            43158..>44156
                     /locus_tag="PLL59_01330"
     CDS             43158..>44156
                     /locus_tag="PLL59_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HMY22471.1"
                     /translation="MQIFNYGIDTLSVAKALALANGNAKGVINDAAKEKIKTSQQNVQ
                     QIVENHDTVYGINTGFGILSNKKISEEDTQTLQYKILQSHSVGVGNAIPNHIAKLMLI
                     TKLQSLAQGFSGVQLSTLERILWHIDNDIIPIVPEKGSVGASGDLAPLSHLFLPLIGL
                     GEVMYKGKTVKTSMLLKQENLSPITLGPKEGLALINGTQFILSFAVAAIQRMHNCLEV
                     ADIIGAMSLEALQGTKAPFDERLHQLRPFKGNQLVAQRLRLLLHNSEIMQNHEDCERV
                     QDPYSLRCMPQVHGASRNAWLHLKELTEIELNAVTDNPIIFNADDTISGGNFHGQPL"
BASE COUNT        14828 a         7709 c         7102 g        14517 t
ORIGIN      
        1 catcgtgccg ccacttaatg ctttgccgag cactaaaata tctggtttta cagactcata
       61 atcgcatgcc atcattttac cagtacgcgc aataccagtt tgcacttcat cagcaataaa
      121 aagtacatta tgtttagaac aaagtgcttt tacattagac aaataagctt catcaggcac
      181 gacaacgcca gcttcaccct gaataggttc agccataaaa gctgctacat ttttatcttg
      241 caatgctgct tccaatgcgg caatatcatt atagggaatt ttgacaattc caggcacgta
      301 aggaccaaaa ccaccatagg aagacggatc tgtagatgat gaaatagccg caacagatcg
      361 accccaaaaa ttattttcac aaacaatgat tatcgcttta tttttctcga tacctttaac
      421 atcataaccc caacgacgtg ctaatttgaa ggctgtttca gcagcttcta cacctgtgtt
      481 catgggcaat actttgtcat atcctaaaag tgtagtgatg tattctgcat aaacacctaa
      541 tagattatta tgaaacgctc tagaagtaag agttaacact gctgcttgct cttgcatcac
      601 ctttattatt cttggatggc aatgaccttg gttgacggct gagtatgcag ataaaaaatc
      661 taaatattgt tttccttcaa catcccaaac atgaacgcct ttgcctttag acaatacaac
      721 tggtaaagga tgataattat gtgcaccata ttttaattcc aaatcaataa gttgttgtga
      781 atgtaatgga ttcatagcta taacttttta tatacaaata tatactaaaa aaaagagaat
      841 agttagttgt tataacaact atattttatt caaaatgaaa aaaactcaaa gtgaaaacga
      901 aaaaataaaa acataaaaca atatataaaa tcgtcgaaaa aataggataa gcaaagaaaa
      961 ctataatact tattcgttaa ttgtgcgtaa ttttatattg tacaagtttg caatagctta
     1021 ttattttgta gtataaatgt tatatttcaa ttgaattaat cgtaaagtta cgcttgaaaa
     1081 aaatgaagat caattacata ctttcctttc ctgcaccaca tactcattat gttgaagtag
     1141 agatgcatat tagtgatatc gcgaataata atttacaatt aaagatggca gtttggacgc
     1201 ctggcagtta cctcattcgc gaatttcaaa aaaacataga cttcattgaa tataaagctg
     1261 aaaataattt atggaaacgt ttagaaaaat ctgataaaaa cacatggttg ctcaatgtcg
     1321 aaaataggaa tgaaatcacg ttgagatatg ctgtttatag ctttgaatat tctgtacgta
     1381 caaattttgt tgacacaaca catgctttaa ttaatggcgc ttccactttt ttatatattg
     1441 atggtgcaga agataccgca ttaaatataa acattcatcc ttttgatgaa tggcaacaaa
     1501 tttccacagc tttgccacaa gccggcgaca acaaatggct gagaacggca gcaaacattg
     1561 atgaattaat agattcgcca atagaaattg gcaatcacac atcatatttt tttcaagctg
     1621 cttccattca gcatgagttg gccatctatg gagaaagcaa tattgacatt gaaaaaataa
     1681 tcgaagattt aaaaaaaata atcgaagaag aagtaaagat atttggcagt catccatgtg
     1741 aaaaatatgt ttttatcatt caccatacgg atagtagtta cggcggttta gagcatttaa
     1801 actcatctgt aaatcatatt acaagatgga gttatgacaa agaaaattat cagcgttcta
     1861 ttagtttatt agcacatgaa tattttcatt tgtggaatgt aaaacgcatc agaccttcga
     1921 cattaattcc atataattac aactcggaaa actataccga tttactttgg ttttttgaag
     1981 gcataacaag ttattatgac gattatgtat gctatcgtgc aggtgtaact cctttagata
     2041 gctttttaaa aattattgaa aaaaacatta atgacgtaat taacaacaca ggatttaatg
     2101 tacaaacact gtcagaatcg agctttgaca cttggctgaa atactacaga agaaatgaaa
     2161 acagtattaa tgctcatgtc aactattaca cacaaggtgc tttgattaca atgcttatag
     2221 accttagtat tatccatgct tcgaatgggc agaaatcttt agatgatgtt atgcgcacgc
     2281 tatatgatgc atatcttaaa caaaattaca aaggcattac agaagaagat attatactag
     2341 caatcgatag tgtttgtcct attaattcca aaaatttatt cgaccaatat ttaagaagca
     2401 caacaccaat gcatgtaatc gacttttttg aaaacatagg aattgaagca aatgaaaaaa
     2461 gtgatgaaaa tgtaatatat ttaggtttag gtttttcatg gaaagaaaat aagctcatga
     2521 taacgcaagt tgacaaacaa tatggtgcat caattggtgg attaagtgca gaagatgaaa
     2581 ttattgctat tgatggtttt agagttaata aaaactacaa aaacatatat gcccacaaaa
     2641 aagtgaacga cacaattgaa gtaacaattg cgaggcaagg catactaaag acattacaag
     2701 tgccgttaac agcagacaga tttaaaaaaa ttactttaca aaaactaagc aatcctaacg
     2761 aatcacaaca aatcaaactt gaaaaatggt tagggaaaaa ttaagcatat gagaataaaa
     2821 ctattaattt taatcattat ttcatttcta tttacagaaa taccaacagc aaatgctaga
     2881 ccgagaaggc ccaaaagttg tgtaaaaaaa gtattgttgc ctggtcacag caaacctgta
     2941 aaatgcagta aagctcgtaa aattaaaaat agatattttt aaaaaacata aactatgaaa
     3001 aaatcaaaat tactcatcat aatgctattc gcatgcaatg gtgtattcgg acaatcattt
     3061 ttaggtggac acttttccac gagagaagca ttattgagca acaccttaaa tccagcagct
     3121 ggtatagcag gagaaatgaa atggcaagtc aattttttag gattaagctc agaagttggc
     3181 aacaactatt tttccatcaa cggtagtcta aaaggtattg ccaacaactg ggacaaagaa
     3241 aaatacatag gacaaaactt agatggcaaa cgcaaggaat tgcacgcaaa cattggtctc
     3301 gtagggccaa gtggctttgt gcgtattaaa caaaataatg caattcattt tggcatacgc
     3361 gccagagccg tattgacagt aaatgacatc aaccaagatt ttgtctattc tatgtataat
     3421 cattttgaag aaatattaca atggttgccc aacttcaatg atgagcgtgc tactgcagcg
     3481 gccaatgttt attcagaaat atatgctgga tacgcacgca ctataaaaat tggagaacgc
     3541 catgctgtac atctaggact taccgcaaaa atgaattcca atattttcaa tgctcagttt
     3601 gttgcaaacg acatcgactt taataaaatt gttacaagtc caacggacag ttttatcaat
     3661 gtaggtaatt ctaagttcga tttatttgtt tcagatgtca tcgacgatgg attcaaatac
     3721 aaattcggtg caaatggttt tggcgttgat cttgatatta tctatgaata taaattaaaa
     3781 aatagcaatg agcattttgt tatggctggt atctcattaa atgattttgg atttaacacg
     3841 tataaactag gcaaaaacag tcgttcattt gttggcaatg gtcgcgacat tcctgcatca
     3901 catctaactg atgcatttgg cgaaacaaat aatattgatg aagtattgga ttcattaggc
     3961 acacgtaccg tgccaactgg tactaaaaaa ataaaaacac cggctacagt aaacgttttt
     4021 gctgatgttc gagtggtaaa aatgttttac atcaatgcca atatgcaatt caatccttat
     4081 agctttaaaa agggaactgc tgttgccaat ttaccaatag acattacagt tacaccacgt
     4141 tttgaacaca gaattgcatc tgtatatttg ccaatcaatt acaatcagta cagtggtttt
     4201 aacatgggcg caggttttcg cattgggcag tttactttag gcagtagtac tattattact
     4261 tcgtttgcca aaaagaaatt tacaggtgtt gacttctaca tgaatatcgg atttggtgct
     4321 tcagaaaaaa taaagaaaag taaaaagaaa gaaaaagcct aaagcatcgc tcaaattata
     4381 aggtaaggtt tcatctatgt taaaaaacag taagttataa ggatttaaca cttatattaa
     4441 cacatatcat caaaacttgt attaagtttg aataagattt ctatgtcagt agaaatataa
     4501 tcaccctttc atcgccgtac aatatttatt ttatgaaagg agtttttatt ttaatcgctg
     4561 ttattttact aagtattttc actactgata gctttgctaa atgcaaaaaa tataaaaaat
     4621 caaaatctag tagcaatatt tatgtgagca aaaaatacaa aggacattcc tgtccaaagc
     4681 cacgtaaaat aatcaatgct gtttatttct aagctaataa ttctgttata aattgctggc
     4741 aagcaaaacc aggattttca gttttcataa agttttcgcc aataagaaaa ccttgaaagc
     4801 catgctgttt aaattgtttt aaagtagcga cagaagaaat accactttct gcaactttaa
     4861 caaatgaatt tccaagtttt tctgacatgc tgatagattg ttcaatatct acttcaaatg
     4921 ttttcaaatt tctgttgtta atgcctacca tatcaacatc agcacatacg tgctgtaatt
     4981 ctgattcgtc atgtagttcc aacaatactt ctaaattaag gctatgtgca atggtagtaa
     5041 aatttttcac ttcttgcggt gtaagtatcg cagcaattaa aagtacaaca tcagcaccaa
     5101 ttgattttgc ctcataaaac tggtactcat caatcatgaa atctttgcgc aaaagtggaa
     5161 tattaaccgc atttcttata gaaattaaat cttgtactgt gccaccaaaa aacaaattgt
     5221 ctgtaagtat agaactacct gaaacaccag ctgcttcata ttgtttgact atatcaagtg
     5281 ttgtatattt atcatttata atgccttttg aaggtgactt tcgtttatat tctgcaataa
     5341 tgccagtagc gttttcttgg ataagttttt gacttaaaga aagtgttggt ctttcgaaaa
     5401 aaatactttc ttccaattgt tttattgaag tcaaagattt tgcttggaca atttcatttt
     5461 ttttgtgtgc gactatttta tcaagaatat tcatataacg aaatgaaaaa ttaagacgtt
     5521 taaattatct ttttatttaa atgattttaa tttcagaaaa caatcataag cactaccact
     5581 tagcaagcta tgcctagctt gttgtatcgc tgctgaatat gaattctgct catacaaaga
     5641 aattcccaaa gcagcattgg caatgactac attattttgt gcttctgtac catttccttt
     5701 cagtatgttt ataaaatgct ttgcattttg tgacactgtt gtaccaccaa acaattcagc
     5761 tgctttgctg tttttaaatc ctaaatcatg tgcactcgac acttgtatat tttttcgata
     5821 cagaatttta gtatcggctg ttagcgatat ttcatcgtaa ccatctaatg catgtactat
     5881 agcaaaattt acgtcacgta attttaaaac ctcacgatat aattttgcaa tcgaatcact
     5941 atacacaccg atgacttgca ccttgggttt tgcaggattt accaaaggtc ctaaaatatt
     6001 aaatattgtt ttcacctgca gttgttttct aacattagca acattcttca tagcaggatg
     6061 aaataaaggt gcatgtaaaa aagtaatatt tgcagcctct aattgctcgt ttagctcact
     6121 ttgattttta gcaaaagaaa tacctgcatg ctctaaaaca ttagaagaac cagacacaga
     6181 tgacacactg tagttgccat gtttggctac tttaacgcct gcgccggcac aaacaaaaca
     6241 tgctaaagta gaaatattaa aagtattttt tccatcgcca ccagtaccaa caatatctaa
     6301 cacattattt gatttgaatt taatcggtgt tgccaactcg agcatagctt tgctaaaacc
     6361 attcaactct tcggcagtcg gcagtcgcat attaaatata gttagaaaag cagcaatttg
     6421 aatttcgttt tgctttcccg atgcaatgtc tttcattacg ctataggctt cttgtgccgt
     6481 taacacattg ccatcaatta gatattgtaa ggtttctttc atttcagatt tttattctat
     6541 cgtataaatt taattccgca aattattttc aatcaagcat aaatgactca acatttaaac
     6601 aaccaatttt caataataat ttttccttgt ggcgtcatta cactctctgg atgaaactgt
     6661 acaccacgaa cattataagt ttcgtgacaa attgcttgca caataccatc atcatcgatg
     6721 gctgtcactt ttaaaggtga aattacattt ttaattgccc acgaatgata tctaccactt
     6781 tgaaattcaa tatctaagcc atcaaataaa atatcatgtg ctattacttt tgtattttta
     6841 ggtttgccat gtataacctg accaacattg tacaattcag caccaaacac ctgacctatt
     6901 gcttgatgtc caaggcacac acctaagatt gattttgttg gtgcgtatgc tttgataagg
     6961 tctaataaaa caccagcttc ttctggaatt cctggccctg gtgacaagag aattttatca
     7021 tatttatcga catctgctaa tgtaatttta tcattgcgaa acacatcaca ttttacgtta
     7081 aatgattcaa tataatgcac caaattatag gtaaatgaat cgtaattatc taaaactaaa
     7141 atatctttca taatttttat ttattcaata taagcttacc tatagtgtta taaagtgttt
     7201 gctaatgcta tcgctttatt taatgctgct aatttatttt caacttcttg taactcactt
     7261 tcaggtactg aatctgctac aacacctgct cctgcttgaa atgttagtgt attgtcttta
     7321 ctcaaaaaac tacgtatcat aatagcatga ttgaaatcac cattaaaatt aagatgtcca
     7381 atacaaccac cataaaaact gcgtgacgtt ttttcataat tatcaatcag ctgcattgct
     7441 ttatatttcg gtgcgccact caaagtgcca gcaggaaacg tatcgccaat catctttatt
     7501 ttattggcat tttcatcaat ttctgaagtg actttagata ctaaatggat tacatgtgag
     7561 taatactgta cttcgcgata tacttcgact gttgtatttt ttccaccacg cgacaaatcg
     7621 ttccgcgcca aatcaactaa catgatatgt tctgcatttt ccttcggatc atctgctaat
     7681 ttttttgcca gtgctgcatc tttaacatcg tcgccagtgc gtcgaaacgt gcctgcaatt
     7741 ggatgaatag ttactttatt atttttaata atgaattgtg attctggtga tgaacccatt
     7801 aatcggaatg attccaaatc aaaataaaaa caatatggag atggattgac tgaacgtaaa
     7861 gcacgataaa cattgaaatc atcgcctcta taattttgtt gaaactgacg cgacaacaca
     7921 atctgaaata catctccacg atagcaggat tgtttgcctt tattaaccat ttcgagatat
     7981 tcagcatcgt tcaaattaga tgttttctcc gcatcacatg caaaggtatc taattgcaag
     8041 ggtttgtatt tgataaaact ttctatatca tctaaagttg acgtatcatt ggtaacttgt
     8101 tcaatttcat ttttaataat gaacaactct tgattaaaat gattaaaagc aatgatatat
     8161 tggtagcaga aatactgtgc aattggaatt gctggtgctg gtttatttaa ttgaatatct
     8221 tcaaaatatt gcaccacatc ataagtcata taaccaaata aaccattagt aataattggc
     8281 aattccgaat aactaaaatt ggaaaatttt gatataaaat cagaaattaa atcacataat
     8341 ttattttctt tcaaactaat tatttccgaa tttctatctg gcaaattgat tataaattta
     8401 tcagattcca ctttaaatcc tgcgattggt tgaaagcaga tataggaaaa attatgctca
     8461 tcaccatgat aatcggagct ttccagcaaa atgcaatttt tatatttcgc acgcaaacgc
     8521 aggtaaatac taacaggtgt cagcatgtct gcaattattt ttttatgtga tgttttaaaa
     8581 ttatgtttca tttattataa acattaatgt atcattatac aaatgctgtg cgttcacttt
     8641 aaaatcgtat aaaaaaagct cgcttttaaa ggcgagctaa tataatacat ttggaaatag
     8701 cattagtaca cgcctattgt cgtttactat accaccacca aaatttattt attgtcaatc
     8761 cagcgttcat taagacaaat atatacaaag gaaattaaaa agcaaattta tttagatttt
     8821 attaatgtag aaaaatctat acacgattga agttctgtta gtaaactaaa gcattccttt
     8881 cgtacttggc aactcattgc catctcgatg cacagccatt tttattgatt ttgcaaccga
     8941 tttgaaaatt gcttcaatct tatggtgttc attatcgcct tctgctttaa tattcaaatt
     9001 acatttggct gcatcagcaa atgatttcca aaaatggaaa aacatttcag ttggcatgtc
     9061 tcctactttt tctctattga atttgacttt ccaaaccagc caaggtctgc ctgagaaatc
     9121 aattgctgtt tgcgctaaca catcatccat tggcaataaa aaaccataac gagcaattcc
     9181 ttttttatta cctaaagctt gtaaaaatgc ttcgccaagt gcaattgcag tatcctcaat
     9241 agtatgatgt tcgtcgatat gcaaatcgcc ttttactttt atgtcaatat cgcaattagc
     9301 atgcttagat aactgttcga gcatgtgatc aaaaaagcct aaacctgttt taatattcga
     9361 cttaccattg ccatctaaat ttaatttaat tgcaatcttt gtttcatttg tattccgttg
     9421 aatagtcgca attctttgtg gtgtagtcag tagttcatat atgcttttcc aattgttggt
     9481 ttttaaagca ataaatgtat tgagttcctt attatttaat tttttatcgt ccgttttatc
     9541 agcacttcta ccaattaaaa ttccttttgc accaatattt tttgccaatt gtatgtctgt
     9601 taacctatcg ccgattacga aagaattttt taagtcatat tttgaagcgt ctaaatattt
     9661 tgtcaacaag ccagtattcg gcttgcgtgt aggtgcattg tcttttgcaa aagttttatc
     9721 tataaaaata tcatcgaata taattccttc tgtttcaaaa actgacaaca ttaagttgtg
     9781 aacaggccaa aaagtttttt caggaaatga tttagtacct aatccatctt gattcgtaac
     9841 cattactaat ttatagtctg tttcgtcaac aatctttttc aaataagtat taacctgtgg
     9901 aaggaatttt aatttatcaa aactgtccac ctgaaaattg tcttttggtt cttctattaa
     9961 agtaccatct ctatcgataa aaagtatttt ttgcatacac taaattaaac tatataatta
    10021 cgtaaaactt caataaattt ttcgttctct gctcttgtgc caacagacac gcgcaaacag
    10081 ccttttaaca atggtgcact atctctattg cgcacaataa ttttatttgc aatgagatat
    10141 ttatacaaat catttgcatt ttctacttta aataaaacat aattcgcatc acttgcaaat
    10201 acttgttgaa taaaagaagt ttttgccaat tcatcttgta tatattttct ccctgcattt
    10261 aaagttgcaa caaattcatt ttttttgtca atatgatgca aggctgtcaa tgctaatgtt
    10321 tgtgaatatt gattgacatt atatggcatt ttaatcgtat tcatataatg aataatagca
    10381 tcatcagcat agagcattcc tatgcgagca tcagccattc cataagcctt agaaaatgtc
    10441 tttatcacta ccaaattatg gaattcactc aaacgatttg tccaacttac tttttcagaa
    10501 aaatcttgat atgcttcatc gattacaaca atacctttaa aatgattcaa tattatttcg
    10561 atatcatttt ctttaattaa attacctgtc ggattatttg gagagcaaat aaaaataatt
    10621 ttgatgttat tttctttgct aatgagcggc aatagtgctt caatatttaa ttgaaaattt
    10681 tcagttaaca atacttcatg cacttgtatg ttctgtgcat gtgcggcatg ctcatacatt
    10741 gaaaacacag gcggacaaac cactactgca tcttctttcg attcacaaaa ggtgcgtatc
    10801 aaaacatcaa tacattcatc gctaccatta ccaataaaaa tatggtttgc ttttaaattt
    10861 tctattgatg ctaatgcatt tttcaaattt tgttgcaaag gatctggata acgattgtgt
    10921 aattcattac aaacagagcc aattggattt tcattagcat ctaaaaaaat gccatcatca
    10981 cctgtatatt cagcacgtgc agatgaatat ggtttcatct ttacaatatg cggtcgaatg
    11041 agattatcta ttgtactatt tttcattttt taatcggata gtgactgcat ttttatgcgc
    11101 tgttaaacct tcattttctg ccattaattc tactgctggt cctatgtttt ccaagccagc
    11161 tttttccatt ttttgaaatg ttattttctt ttggaagcta tctaaggaaa caccgctata
    11221 tgctttagca aagccatttg tcggcaaggt atgattggtg ccgcttgcat aatcgccaac
    11281 gctttctggc gtgtaatgac caataaacac agaacctgca ttgcttactt gattcgctaa
    11341 ttcttccgca ttacgtgttt gcaaaatcaa atgttcaggc gcatacacat tgctaaatgc
    11401 aatagcatca tcaagatttt ttaacaatat cattttgcta ttttctaaag ctttttctgc
    11461 aatcgatttt cgtgctaata tttttagttg ttcgtcgatg gcgaaatgta ctaagtttaa
    11521 atacatttct tctgtggtaa caaaaatcac ttggctatcg atgccatgtt ctgcttgtgc
    11581 caataaatct gctgcaataa aatcaggtcg tgattcatca tcgccaataa ctaatacttc
    11641 acttggtcca gctggcatat cgattgctgt gccactttta ttgactaatt gtttagcagc
    11701 tgtaacatat tgattgccgg gtccaaatat tttatatact tgtggtatcg tttgtgtacc
    11761 atatgccatt gccgcaatcg cttgcgcgcc accgacttta aaaattttat gaacgccaca
    11821 taaatttgca gcgtataata ttactggatt tattgttcca tcttgatttg gtggcgaaca
    11881 caataccact tcgctacaac ctgcaatttt tgccggaatt gccagcatca acactgtaga
    11941 aaataatgga gcagtaccac caggaatata cacacctact ttatcaatcg gcaacgattt
    12001 acgccagcaa aaaacaccag cacttgtttc aattatctcg gcgcttgttt gttgttgttg
    12061 atgaaaatat agaatatttt tatacgctat gtcgattgca tttttcaaac caacatcaac
    12121 agtagcgata gcagtagcaa tttcttcttc cgtgaccaaa agattagaaa tacgagcttt
    12181 atcaaatttc tcagcatact caaacaatgc agcgtctcca cgcaatttga tatcatctaa
    12241 aatcggactg accactttct ctatttcttc tagagattgt gttggtcgtt gagaaagttg
    12301 ttgccaagtt tctttacttg gatattttat tacttgcatt accatttata tttaaacata
    12361 cagacaaatt tctagtatgc tatgcatgaa ttaataaatt aatttctcta ttggcatgac
    12421 caaaatacct tcagcaccag cctctttgag ttgttgtatt ttttcccaaa aatcgtgttc
    12481 tgttataacc gaatgcaccg aactccaacc ttcttttgct agcgcctgca cagtaggtga
    12541 gctaattccg ggcaacaagg atatgatttt atctaagctt tcgtttggcg catttaacaa
    12601 aatatattta tttttttgcg ctcgttttac tgcttctaaa cggaataata actgttgtaa
    12661 gatttcttgt ttttcagtcg atagtgattt attttttatt aaaacagctt ctgattgaaa
    12721 aatttgttct acttctttta aaccattact caacaaagtt gatcccgttg aaacgatatc
    12781 acaaatggca tctgccaatc caatgctagg tgctatttcg acagaacctg aaatgatatg
    12841 tatttctgca tggatattat ttttatccaa ataattttgc aatatttttg gataggaagt
    12901 ggctatagat tttcctgata aatcattggc agaattaaaa ttcatagtgc gaggaacggc
    12961 aagcgacaat ctgcatttac caaagcccat tttttttact tcatccactt ttttatccga
    13021 ttctactaat acattttcac caacaatacc gatatcggca acaccatctt gcacataatc
    13081 tggaatatca tcgtcgcgta aaaacaatag ttcgattggg aaatcataag cagcagtgac
    13141 tagcttacca gagcttgttg gaaatgaaat atcacattcg tttatcagct tgatagactc
    13201 atcgcttaag cgaccttttt tttgtatggc tagttttaat ttttgcataa aaacaaaatt
    13261 acttttttaa aaattaagat aatatgatgc tttaattcaa aagcataaat aaataaaata
    13321 acaaattgga aaaataatat agcacactcc tacttggagt gatgacgaaa gtgagcgatt
    13381 gtttgtaatg agaacatcat ttaatcaaag ataatgaatt aatttattca gcatattaag
    13441 tctatgttag aaatatacta tagaaaaata gaatcaaatg gcaagaaata cacaagaaat
    13501 acgcaataaa atgtaatttt atatacacaa ttaggcttat gaaaaaaata cttttaattg
    13561 ccaatttact tatttatata tcgttgagtt cttttgcgat gcaaaatgat gtccttaatt
    13621 tcgacaatta caaagaagaa tttactaagc ggctttggaa acaatatcct gactgggcaa
    13681 ctggaattgg ctatcatgaa tatgacaatg ttttattcgt tccgaatgca ttacagcgca
    13741 caaaagagaa agcatttttt gaacaggaat taagcaaact tcatagtttt tcgctgagca
    13801 cattaactga tgcaaataag attgattaca agattttaga ggactttttc cattacaata
    13861 tttttctaat tgacacctac aaaagttggc aatggaatcc agcagaatat aatgttggtg
    13921 catcattttc ttacatatta tcagaaaact acgaaccatt aagtaagcga ttaaatagtt
    13981 tttatcagaa acttaaaaat gtaccagcct attatacagc agccaaatta aatatacaac
    14041 atccaacaaa agaacatgcg caactcgcca ttgaccagaa tgaaggcact ttaacaattt
    14101 ttgaaaaaga cttcatcgat tctgtgaaaa cgatgcattt aaaacctgct gtggaagaag
    14161 cttatttaaa aagagcagaa gctgccgcaa aatcgattcg atcttatcta cactatctaa
    14221 aaaactataa aaacgaaacg ccaaggagtt ttcgtatcgg cagcgattta tatgcagagc
    14281 gttttaaata tgaaattcaa agtaaatata cagttgatga aatatattta gcagcagaat
    14341 cacgcaaaga atatttgcat gacgaaatgg aaaaattagc cattaaacta tggccaaaat
    14401 attttggtga taaaccgcaa gtaaaggaca aactccagct cataaaaatg gtaattgata
    14461 ctatttctga tcaacatgtt gaaccagcat tgtttaaaca agaaatagag aagcaactac
    14521 cagaactcgc tgcatttgta gagaaaaaaa atctcattta cttagatcct agcaagcctt
    14581 tgaaagtccg aaatgaacct ggctatatgg ctggcgttgc aggtgcatct atgagttcgc
    14641 caggtccata tgaaaaaaat ggaacatctt attataatgt tggcacgctt gatggctgga
    14701 gtgcagaacg ttcggaaagt tatttaagag aatacaataa atatgtattg caaattttaa
    14761 atatacacga agcaattcct gggcattatg tacagttagt gtatagcaat aagtcgcctt
    14821 ctatcatcaa atcaattttg ggaaatggag caatgattga aggctgggca gtttattccg
    14881 agttgatgat gatggaaaat ggctatggca ataatgaaga tgagatgtgg ctaatgtatt
    14941 acaaatggaa tttaagaaca gtatgcaata ccattttaga tatcagcgtg cacaccaaag
    15001 acatgagtaa agaagccgca ttagatttat taataaatca ggcatttcag caacaagcag
    15061 aggcagaagg caaatggaaa agagtacaag ttacaagtgt gcaactaaca agttatttca
    15121 ctggatttta tgaaatattg caattacgtg aagcttataa attgcaaaaa ggagctcaat
    15181 attccgtaaa agaattcaat gaaaagattt taagttatgg aagtgcaccc gtaaaatata
    15241 tcgcggaaat gatgttaaag aagtaaaaaa ataatttgtt aaatatttgc agaattcaaa
    15301 aaaaataatc aacttaaagt cataaaaaga ttgcagtctt tattagttaa aagcagacta
    15361 aaatagttct aaagcgccta atgggcgctt ttcttttttt ctattttttt gagaaaatgt
    15421 tatatcttca aggaaaccaa atccaacaat gaaaccaata ttcattttat ttttttctgt
    15481 tattattttt tgtttctcct gtaaaaaaga aaactcaaat gatagaaaaa ttccaattcc
    15541 agaccagaca gaaataaata aattgaagat taatcaattg caaattttag gcagccataa
    15601 tagttatcac aaacacatgg acaataggct gtttgcattt ttaggtaaca tcaatttttt
    15661 attgcccgaa aaatataaag tagatgcatt agactatatg cacgaaagca ttccagacca
    15721 attgaacaaa tacggcatgc gcagttttga aattgatatt tataacgatc cgaatggtgg
    15781 aaggttttat aataggaatg gcaatagatt tgtacatatg accgttgaat cgcatattgc
    15841 agatttacag caaccaggtt tgaaaatatt acacattcca gatattgact acgaaacgca
    15901 ttattacact ttcaaaaaaa tgttaaccgc attaaatgaa tggtctgatg cacatcccaa
    15961 tcatttgcca ttgtttattt tagtggaaac gaaagaagaa actgttggtg atgttttagg
    16021 atttttagga tttcaacaag caattaaata cacgcctgcg cttgcgagtg ctatcgacga
    16081 tgaaataaaa agtgtgtttg gtgctgactt aaaaaaaata attacgcctg accaaatacg
    16141 aggtacttat gctacactag aagaagctgt gctggcaaaa aattggccga cagtaggcgc
    16201 cagcagaggc aaatttattt ttgtgatgca aggtggtgcc gaagacgaat atcttgttgc
    16261 acatccttcg ttgcaaggca gagccatgtt tataatgtct gagccaggca agcctgaagc
    16321 cgcatttatc ggatatgata atccaattga aaaacaagct gaaactatac aagctgtgca
    16381 agcaggatat atggtgcgca ctagaagtga cgagcctaat gtgcagaata aaaatggaag
    16441 ctacgagcaa caacatgctg catttagcag tggcgctcaa atgatttcga cagattatta
    16501 cagaccagac acaagacatg catcttcgcc aagaatgttt actaattatt cgtgtaattt
    16561 tccgaataat gaacttgcac gaattaatcc tatttcggca gctgacaaac aaaacatagg
    16621 aattattgca gaataattta taaatgcatc aaactattta tgagaattat cttttatgtt
    16681 ttcattttat taactgccaa tggcattgct caagaaaata aaattattga gcaattaaag
    16741 ataaatcaaa ttcaaacgat aggaagtcat aatagttatc atttacgagc caataaattt
    16801 gttcttcgtt ttctgaaagg ccttgccgcg attttaccaa aagaatacaa tccgaaggat
    16861 ttagattatg cacatgaacc actatttacg caattagata gttttcattt gcgtagtttt
    16921 gaaatcgatg tatatgctga tccaaatggt ggacattttt acaaacgcaa aaaaaataat
    16981 ttattaggca aacctaaaat atcaaaaata acagcgctaa aagagccagg atttaaagtg
    17041 atgcatattc ctgatattga ctataacaca cactatttca cattcaaaag tatgttgagt
    17101 gattttaaaa aatggagcga ttcgcaccct aatcatttgc ctatttatat tatgattgaa
    17161 tgcaaagaag aaactattgc cgataaaata aagtcatttc atttcacaaa agccatacaa
    17221 ttcacgccag cattatgcga tgacttagac aatgaagtaa aagaaatttt tggtgaaagt
    17281 ttgcaaaagg taatcacacc tgatgaggta cgcgatacgt ttgctacact aagagatgca
    17341 gttttagcag ttaattttcc gacattagat aaagcacgtg gcaagtttat attcatagtc
    17401 atgcagcaaa gagatactta tactaaaaat catttttcat taaaagacag agccatgttt
    17461 gtatttagcg atgccaacgc tgctgaggct gcatttatca aatatgatga tgcattacat
    17521 gatgaaaatg aaatcaaaaa agctgttcaa ataggattta ttgtgcgtac aagagctgat
    17581 ggtcctaata ttcaaaatcg aagtggtgac tattcacaac aagacgcagc ctttcgcagt
    17641 ggcgctcaaa taatttctac agattattat cgaccagata ttcgacacca aaaaaaacct
    17701 aggaaattca ctaattatgc tacgcgattt ataggaaatg atattgcaag aattaatcca
    17761 atcaacagca ccaattatac catagaagaa aaataaatgc ttactgttgg attgtaagtt
    17821 ttgacaaata ttgttgttca tcagcaataa tactaacaac atatatgcca tttgctaagt
    17881 caacattaat atctatattt tctttatttt ctacaagtac tgaaaaaaca tttttcccta
    17941 caacatcagt aataacaaca cgtacagctt tatattgtaa tgtacgtggc agttgaattg
    18001 taaaatttcc tttagaagga ttgggaaata attcaaatga ttgtaccaca ttatttctaa
    18061 tagaagtcac gatttgttga ttcatgattc tatataaaaa atcagttgtt ttctgcaata
    18121 tcataggtcg agctacggta tcactaactt gatcaacagg atgacctaat cgatttacag
    18181 gataaaattc agcataagtt ccattgtttc tgattttagg aaaaatttta ttagcgccat
    18241 aaaaattacc caaccaacat gccactgtaa cgaagcaacc cacatcgaaa ggcactgtca
    18301 aatctggatt attatgaaaa ccgagatatt caatatctct attgttattc atgtaattta
    18361 agtcagcaat tgcgccactg caactcacaa tcgcttttat tcctttcatc gagtatccaa
    18421 gatttccact atttccatcg agtccgccca tttctctgaa tgccactcta aattgcggat
    18481 tcatctctaa tgtgtcatct aaaaatacgg tatgtaatgc agtaattgcg ccagcagatg
    18541 agccataaat aaatatggaa ttggtatcca ctttatactg attataattt gttgcgacat
    18601 ctttttttac ataacgaatt gcggctttta aatcatgaga ggcgcgtgta atggcacgat
    18661 accaattata catatcagca aaatcaacaa atggagaaat aagtgtagtt tgttgcacac
    18721 ggtattcgat attggctgtg acatagcctt tctttgcaaa gaattcagca atctcatcaa
    18781 tagattgatc ctgtctatca ccgcctacaa atgagccgcc atgtacaaaa aaaataattg
    18841 gacgtaatgc tgctgtatca ctttgtggtt catacacatc cattaataaa tcggtcaatt
    18901 gatttttatt atcgtaattg cgaccataca ttacgtcagt atgttttgag atactaaaca
    18961 tctcatcttt atatctaatt tgagaaaaag cagataagga gatacagaaa aataataatg
    19021 taaaaaaacg tctcatcttt attgattaca caataaatat aagattttgt ttattgaaaa
    19081 tgaaattaaa aattgatagc acagctttca ttttccattt ttaaaaaaaa ataataagtc
    19141 gtgtaaatta gtattgtgtg ttatacacca agtagctttt ttggaaatat ttaagcaaat
    19201 aaaaaaaatg ttatttcaaa agaaaaagtc aattatttgc tcatcaacca cgctttgaaa
    19261 acatcaaaat ttgcagacat tggaatggac aactttttgc cattaattat ggattgcaat
    19321 ctttctggta ttgctacttc ttcaccaatt aatggtgcaa caagttcgcc aaatttaaca
    19381 ggatgcgcag tcgataaaat cacaccattg atataatatg gattcaaatc attatctaat
    19441 acaaactctt ccatgcctaa atatccaact gctgtatgag ggcacataat ataattatat
    19501 tcttcaaaga tatttgaaat gcagcttgct gtttgctcgt tattaaatga atatgcggca
    19561 atctcttcgc gaactttttg tacatcattt gcaaatatat ccatcattcg tgcaaaattg
    19621 cttggatttc caacgtccat tgcattcgac aaagtttgga cagaacgctt aggttcaaaa
    19681 acaccagttt cgtgatatac tgtaaagatg tcatttgaat tatttgctgc aatcattttt
    19741 gtgatgggca agcccatttt tttagcaaac aatcctgctg ttaaattgcc gaaattacca
    19801 gaaggaacac tgaaatacaa tggcaatgat ttatcatcga gttgtttata cgcataaaaa
    19861 tagtagaatg attgcggaat caaacgtgca atatttattg agtttgcaga cgacaaagtt
    19921 aatttctcat ttaattcttt atctaaaaaa gcttgtttta ctaatgcttg gcaatcatcg
    19981 aaagtgccat caatttcgat agcagttata tttgcgccaa gtgttgtaaa ttgtttttct
    20041 tgaatatcgc tcacctttcc tgctggatat agaatggtaa cgtgtatgcc ttctacattc
    20101 agaaagccat gtgccaccgc cgaacctgta tcgccagaag ttgccactaa aatattgact
    20161 tctttatcat tgtcttttaa gaaataaccc atcaatctgc ccataaatct tgcgccaaaa
    20221 tctttgaacg ctaatgttgg gccatcgaat aattctaata cagaatagtc accgacttca
    20281 attaaaggag catcaaagtt gtaagcatct tggataattt tattcagtgc ttcatcaggt
    20341 actgcatcgc caatgagatg tttggccaca tgaaatgcca tttcacaaaa tgtataatta
    20401 tctaaattat cccaaaattt ctgaggtaaa ggatttattt cataaggcat atacaagcca
    20461 ttatccgatg gaaggccttt taatacggct tctttcaagt caacatctgg agaaatgtgt
    20521 tttgtggaat ataatttcat gtgtatattt ttattgtatt tttaaatgtt gcacttcgta
    20581 ttcgctaaca ataattttat tttttttgcg aacattgttg ctcatttatt ataattgatg
    20641 gaaaaattaa tcaataagtg gcaatacttt tggtccttgg ttatttacct tgcttacata
    20701 aacatcagca ccgatattca tttcgttcat gacgtttttc atcgcctctc caactttata
    20761 tgctatttct ttgccatggc acaaggcaaa cacacttggt cctgagccgc taatgctacc
    20821 gcctagagcg cctgcatttt ttgctgcatt tttcatttca taaaaaccag gaataagctt
    20881 gcttctataa ggttctgcaa tgtaatctac taagcttctg ccaattaaat cgtaatcgcc
    20941 gcgcaacaaa ccaaccatca tgccaccaac gtttgcactt tgacgcaagg aatgtgccaa
    21001 cggaatattt ttaggtaata tttcgcgtgc atcttttgtc agaacaatca catgcggatt
    21061 aataacagcg caccacaaat cttcgggcac tggaatttga aggatatcta aaggctcata
    21121 actacgaacc aaaataaaac caccaaataa gcttgctgca acattatcag caatggcggc
    21181 atcgcatgct gctttttctg catgcaataa gaatggcatc aattcttgtt tcgttagttt
    21241 ctcattgcac aacatattga ctgcaaacac acctgctact gcacttgctg cactgctacc
    21301 taaaccactg ccacttggca tcatcttttt caaccaaatt ttgcaaccaa aatccgcttc
    21361 tataaaatct aaataatctt gaatcgcaac agtggcagag ttctttttag catcgcgtgg
    21421 caacacacca tcatcacctt ctatttcttc aatgacaaca cctttttctg cagtaatttc
    21481 catgattagt tcatcgcctg gtttgtcaat ggcaaatcct aacacatcaa aaccacaagc
    21541 cacattggct actgttgcag gtgcaaatac tcttatttta tctccaactt tcattatact
    21601 taagtattta gcaatgcttt taaaaattaa atctcattgc tttttctcta aaatttaatc
    21661 agccatataa tttccgatag taataatttc tgcgaaaacg ccagctgctg tcacttctgc
    21721 accagcgcca ggtcctttaa ttactaaagg attgttttta taacgctctg tagtaaacac
    21781 aatcatgtta tcgctaccac ttaggttgta gaatggatgt tgggcatcga cttgctttaa
    21841 ttcgattgct gcattgccat tttcaatcac ggccatatag cgcaaaacat tacctttgct
    21901 ttctgcatct tttttcattg actcatacaa tgcatcaagt tctggaagtt tatcaaggaa
    21961 agttttcaca tctacttcgc gtaaattttc aggcactaaa ttttctactt tgatatcgct
    22021 taattccatt tgtgcaccgc tatctctgga aagaattaat gcttttcgtg cgacatctaa
    22081 acctgataaa tcgtcacgtg gatcaggctc tgtaaagcct tttgctttag catcaagcac
    22141 aacatcagaa aattttaagt ctcctttata cgtattaaaa ataaaggaca aagtgccaga
    22201 aagcacagct tctattttta aaattttatc tcctgaattc aataaagaat gcaatacgcc
    22261 aataactggt aaacctgcac caacattggt ttcgtacata aactgagcat tagatttttt
    22321 tgctaattca cgtaggcgaa catatttatc ttgactaagc gtattggcaa ttttattagg
    22381 tgttacaatt gacacgctct ttttcaaaag tttttcataa aactctacca catttttgct
    22441 ggcagaacaa tcaacaaata cagagttggc aaagtttaag tctattattt tatttgtaaa
    22501 attatctaaa tcagacttct ctcctttttc gacgagtgta gtttcccagt ttgataaatc
    22561 gatgccattg ctatctatca acattttatt agagttgata ataccagtaa catttatctg
    22621 catggatttc tcttttttga gatattcaaa ctgattgtga atttgtttta ataaagtttt
    22681 gccaatcaag cctgttggtc cgaccaagaa aatatttaca gaatgtacat cactttcaaa
    22741 aaagatttcg tgtaatgcgt ttaatgcttt tgcttcattg tgtttatcaa taacagcagt
    22801 aatatttaat tccgatgagc cttgtgcaat tgcatttacg ttaataccat tttttccaag
    22861 tgctttaaac attttagcag atacgcctgg cacgttggac atattttcgc cgataacggc
    22921 aattatagaa ttatttgttt cgactactaa tggtgcaatt tgtctatttt gaatttcata
    22981 cctaaattct tcttctacac ttttttgtgc tttttttatt tcattaggca caataccaaa
    23041 acaaatcgaa tgttcagatg agccttgtgt caggaagatg atattaattc ctgcgcgaga
    23101 caagcaacca aataatcttg atgcaatacc cactacacca accataccag tgccttccat
    23161 gcgcaacacg gcgatatcag aaattgacga aataccttta atgtcattat ttgacttacc
    23221 agaatcttta gaaacgaaag tacctggatg cgtaggattg aaagtatttt taatgaccaa
    23281 aggaatattt ttgatataag caggctgaat acttggcgga taaataacct ttgcaccaaa
    23341 atgtgataat tccatggctt ctttataaga caaagtagga atagtgaaag cctgttctac
    23401 acgacgaggg tcggcagtta gaacgccatc aacatctgtc catatttcaa tggcatctgc
    23461 ttttagtgca gcgccaaaaa ttgctgccgt aaaatctgag cctccacgac cgagcgtggt
    23521 tacatctcca tgttcgttac agccaataaa accggtgact acggcaacgc cgtttactgc
    23581 ttcaaagaaa tcaagaatat ttttattagt tttttcaaaa tttacttttg ccgataaata
    23641 attagaatct gtgcggataa gttttgttgc atttacataa atgccattta agccagcact
    23701 tcgaagagcg ccagcaacga tgaatgatga gaaacgctcg ccatacgaca ctaagttggc
    23761 taatgttttg tctgataatt cgcctaacaa attaatgcca ttcaagatgt cttctacacg
    23821 cagcatgctt tcgacaaact cttccatcat cactgctttg atttcttcgt cgctaaccaa
    23881 ctctgcaatt gctaatcgat gacgttcttt aaaactctca aaaatttcga gataagtttt
    23941 attgtctttt ttttctgcca gttttccggc attaattaat tgatctgtaa ctttactaaa
    24001 tgcagaacat actaatacga cttcttcatt tttgtctaat tcagcacgta ctatttcgat
    24061 aatacgtttt atattgtctg ttgaacctac ggaagagcct ccaaatttta aaaccttcat
    24121 gttaaactaa ttgcattgtt tgaatgattt agcagctaaa tcaaggaaac aaaaataagt
    24181 aattggcaat actattctta tataaaaaaa ctacacagcg ttaagtatta attaaatgtt
    24241 gaatagaatg atagcatttt ggataaatct caataaaaaa tgaagaaaaa agaataatat
    24301 aacatattta aaattataac aaaaaaagta accgagttga tgtaggaaca tacaactcgg
    24361 tactgaaatt gcccatcaaa ccccaataaa aacgatgggc ttttcgataa atttatttat
    24421 tttctaatac atacattaaa ttagaatcct gctgtaggac aaaagattgg ttttgtaggt
    24481 tttttatgtt ctttgaataa taaaccagaa taagaaatag aaatttcacc actgccatta
    24541 aatctgctta ctttggtaag tttagaaaca ttgatatcgt agttaacggt gatattaaat
    24601 cctttatatt ccacttttac agcaggcacg acggcatcat tgactcgata ccacacacca
    24661 aaattgatac ctaacatttc ttttctattg cgcacaagtc catatcttgc aaagctgcct
    24721 accatgattt ccatactagg gccttgtttt tgaaaaatag cagaaggcaa taaagaccag
    24781 ctacctttta atgaaacttc accaccagca aatgcagtaa aacgcgtgta taatctgtgc
    24841 tcagtattgt cataaaatga aacatttgga cgtgtcaaat taaatgctga aaaacttaca
    24901 taaacattat tgtgttcttt tggcgaatag gtatataaac caccaattcc taagttataa
    24961 tctaattgtt tagtatttaa accagactct cctggattaa taccttgatc aaatccgtcg
    25021 tatccattga attgattacc aaaagttgct ttacttaaat caaatctgcg cataacaagg
    25081 ctattttgaa aaccaacgcc aattatatgt tgtcgtttta cgtctaatac ttgcaaatac
    25141 gaaacattca aattaaacaa gctcgtatta aagctagtag agcctgctct atcatgaata
    25201 atatctacgc caacacctac aatagagtta ggtcttttat tttttagcaa agaaacttgt
    25261 cctgtagcac ctactgtttg aaaaggtgcg gacacgctac tccattggtt tttatatgcc
    25321 gcagcaacat aataatcgtt gttgaagaag ccagtaaatg ctggatttac atttgatggc
    25381 aaggcataaa attgtgacga attaatatcc tgtgatcttg actgataagc caaaatcaac
    25441 agcaaagcgc ctaaaataat atttttcatg tttcaaaaat tagggttata aaatttatgt
    25501 ttcaagggtt gtttttatct caacaaggtg atgtcacctt tcttaatggt acgttcgccg
    25561 ttgatacact ctacatccat ataatagaca aacacaccag gatttaattc ttgtccgttg
    25621 aatgtaccaa accagccaat gttttctgga tcgtttgttt caaacacgat ttgtccccaa
    25681 cgattaaaga tgcgtaattc ttttaccgat gctacacctg gcccatacac tttaaaaata
    25741 tcattttggc catcgccatt tggtgtaaaa gtattaggca catataccat ttttccggca
    25801 caatcttcta ttactctaat gcgtaactta tctgtggctt tgcaaccata ttcagtaatt
    25861 gcggtaacat aatagaaagt atctacctca ggagatgcca ttggtgttgg acattgcgtg
    25921 caagaaagct tatagtcagg actccataaa tacgaaacat tatttggcga agtggcatct
    25981 acttgtactt cttggccttt gatgatgata tgatcttgac cggccgttac agttggcaat
    26041 ggttttacat taaccacaac aggtaatttt tgcatttggc attttccatt ttcaaacaaa
    26101 gcataatacg tagtatttgt tctcggaaat gcgtatggat ctaaagtatt tgtcgtcaaa
    26161 gtcggtgaag acatccaagt aattttagtt gtattcaatg atgtattttc agctaatgca
    26221 tgcagctgaa ctgtatcgcc aatacaaata ttagtgtctg gataagcacg tgcagcaagc
    26281 ttggttttta cggtaatatt taccgtatca tacacgggac attgcggata attataagct
    26341 ttgatgatat actgcgtact agaatcaggt ttagcaatta cattttggct attattaaca
    26401 tttaaaaatc tattcggaga ccaagatatt ctagatggat taccttctac ttgcaaagct
    26461 atttcttctc ctttacaaat taacttatcg tcgatagctg caattactgg cgttgaataa
    26521 acaccgactc gaacactgtc ttttacgata caaccaaaag tattttctgc aaaaaagttt
    26581 attttatacg aacccattcc cactggaata aatgtatcac taacaccgcg agttggaata
    26641 tttgtagagt cagtaaatgt ccagtagaat tcttttattg gcgaatttgg agaagtatat
    26701 aatgagtcat taattgccac actacctgaa ccactacaaa cacttgtttt atctacaccg
    26761 aaataaggta ccatacgagc gacatttact ttttttgtag agatgatatt cacagtgcaa
    26821 ccataatcat cttttaaaac taaagttggc gtaaactcac cttcgcgtct ataagtataa
    26881 gttgattggt tggctaatgt tctatcttca cgcgtgtctc cattaccaaa atcccagatt
    26941 gttgttgttg tatttttaaa ttgagcatca aaagttacgt taaacggtgc acaacctgta
    27001 tctggcataa aattaaaact accagatggg cctgacacga tgatgtattt tggtttgaat
    27061 accgtatcca cgcaattatt attgtcagta acaatcaatt ttacatcgaa tgaatctgaa
    27121 cgtgtataaa caccttgcgg attgtagtta ttgtcattac ttgtatttcc attaccaaaa
    27181 tcccattgaa cagatgacaa ttgtagatta tttggattct gtaattggaa atctgtaatt
    27241 aaatctggac atgttttaaa agttgggctt gcagtaaaat tgacatgtat attcttgatt
    27301 ttcacaaagc ctggttttac catagtattt gaacaaccat ttccatcagt aacagtcagt
    27361 ttaatggtat aatttctttc ttgtgagtat gtatgaatag gaaaagtatc tgtagaagta
    27421 gtgccatcac caaagtccca aaggaatgtt gtcgcatttg cagatgattg attaatgaat
    27481 tttattggtt taccaccaca tacattattt acgtcaatgt caaaatctgc tttcacttca
    27541 gatattgtca caggtttagt tatagaatct ttacagccgt attgattttc tactgttaat
    27601 ttaactggaa acgagcctaa ggcattaaaa gtgtgagata cttcacttcc acttgcagtg
    27661 cttccatcat gaaaattcca agtataattt gacaatggag aaaatctaga ttggctagta
    27721 tcttttaaat ttataattgc tggcacacaa ccactcgctt tatcaaaatt aaaattaggt
    27781 ttaacgccaa tcacggtaat ggtatctttc tgtgttgatt ccatgacgca tccatttcta
    27841 tatttgacaa acattttcac aacgaatttt ccttcgttaa aatatttagc tacatagtta
    27901 tttccgaaca ctaatgttcc attgccaatg tcccataaat aagctacaat attggtatct
    27961 tcaacagcgg tcaacgttac tgttaatggt ttgcaaccca tttttgtaga tgtcgtaaat
    28021 cggaaagaat cgatattaga aaatttaatc agtttcacta aagtatcaat acaacctgtc
    28081 gagtcattcc gtacaactaa agacgcagtt acttgtgtag ttcctggcgc atatgtatgt
    28141 gataaatttt tatctaaagt tgacagaata gtgccatcac caaaatccca agtagcagca
    28201 gtgtgtcctt tagaaaggtt aacaaaatct accttttgac tattacatgt agaagaataa
    28261 ttaaatgccg cttttggata agctacaaaa atttcttgaa catctagtgt atctctacaa
    28321 gtgttattga tagcaataag ttgcacattg tattttcttg gttgagaagg ttccgtataa
    28381 atatgagtag tatttctacc ttgacctgta ccatcaccat aaatccaaat aaattctgtc
    28441 gtaggaatac caccagtagc ggtataagtt accatttctt tgtttacaca aactttatcg
    28501 taatccatag tgccattaaa aggatattta ccacctacat gaattggacc aacaacatca
    28561 accaatgatt cgcatggcgc atcataagta actgttactt tggctgtata atcacctgga
    28621 ttcatatagg tatgaacagg attttctcca gtacctgtag tgccatcgcc aaagtcccaa
    28681 acaatgcttc ttggagtcca attgtgagta agattggcat tgaagaaaac ttttaaatct
    28741 tcgcaacctt ccattttatc tgcagtggca ttgatttggt cagggccaat ccataaatat
    28801 tgaggcatat ttaaaacatc agtacaaccg cttgcagatg taacttccaa cttaacatca
    28861 tacgaacctc tattgggtaa atttacagaa ggtgatgcag atgtgtaggt ttgaaaaaaa
    28921 ccattcggac cactaataaa ccatttataa gaactgccat tttgtgagct attgcttaga
    28981 ttaaccgtat atggcataca agattttaac acatcagctg aaaatgcagc ttttggtgaa
    29041 agattaacgg tcacattttg cgtcattgaa tcgcggcaac cttctagatt tgtagctttt
    29101 agattaattg tataattacc tgtatttgca tagctaatgt ttggaaagac gatatttgag
    29161 atattaccat cgccaaaatt ccaattacaa aaaataggat tataagttga gtttacactt
    29221 ggcgttactg cttggtcagt acataatgga ttgggcacag aagcatttac tttaaagcgt
    29281 tcaattttta ctgcacttgg tattgttttc gttgccgtac aattattttg atctttaact
    29341 gttaatgaga cattgtatgt accgatattg gtatagttgt aagttggatt tgcaatagat
    29401 gaggcatcac catttccaaa attccaagta taagtaacag gcgaagcaga ggtaactgta
    29461 ctttggaata ttgctggaaa aggaagcaaa caagtttgag atttatttac cgtaaaatct
    29521 acagttggtg gtgttgcttt tttgatgaaa tctcgaacgg tttctgtgtt tttacaacca
    29581 tttgcatctg taacaataag agataccgta agattaccgc taaaagtata agtatgtaaa
    29641 gggtttttca cggtttctgt tctaccatcg cccatgtccc acaaccatga agtaattggt
    29701 gcatcaccta cagttgataa atctgaaaac tgaacattaa aaggcacaca acctgtacgt
    29761 ggtgctgcat cgaaacgaga tattggatct ctaaaaactg ttactacttt agtgatagtc
    29821 gaagtttgtg ttcctttata aatcgtcaat ttaatattgt atgtaccagg ctggttaaaa
    29881 atggcactcg gattacactc atcagcagta ttaccattaa tacctaaatc ccaaaaacag
    29941 ctatcttggt ttccggttga agtattatta aaggtaacat acaatggtgc acaaccttta
    30001 tctttactca tagtaaagtt agcattgagc tgtgcatacg aacttaaatg gcttaacata
    30061 agaatgctaa gcacgattaa agtagataca cttctaactg aagacaaaat tagtcttcgc
    30121 gtatagcgaa ataagaaata cattggacat ggggtttatg ttcaatgcaa gtttaaatta
    30181 attattccta attgtcaata gcagattaaa aaaaagttaa ctatatgaat aaaaacttga
    30241 tgtaagtcaa gccaaataaa tagaaatgac aaattattta agatatttgc aacatgaatg
    30301 gaatatgtca tttgtcagtt gtgccaatgc ggaaagaagc tgccgatgct tcggaaatgg
    30361 taaaccaatt actatttgga gaaacatttg acatcataga aaagacagaa aaatggagta
    30421 aaataagaat acatcaagat aaatacgaag gctggattga caacaaacaa tatcttgaac
    30481 aagaaaagaa gaagaacaca aacaaacatt gtgtttctaa tttactttta aacaaaaaag
    30541 gcatacgtta tccattaggt agttatgtag cattcgacac tgctaacaat aataatgaca
    30601 ttattaaaac tgccaaatta tttttgaata cgccttattt atggggcgga aaaacttgtt
    30661 taggtatcga ctgttcaggc tttacacaag ttgtatttag agtacatcaa atacaattat
    30721 tgagagatgc atatcaacaa gccacacaag gaaaaaaaat tgcatttgct aattgtatta
    30781 gtggcgattt agcttttttt aagaacgcaa ataatcgcat tatacatgta ggtattatta
    30841 ttaaaaaaca aaacaagtta gaaattatac acgcatctgg ttgtgtacgt atagatacac
    30901 ttgatgaaca aggcatcttt aatcatactt tagaaagaca cactcatcaa ctcgattgta
    30961 tcagacgagt attgaagtaa gttaataaaa caaaacacct tggataaaaa aaacatagtg
    31021 atattaagtg gcgcaggaat tagtgctgag agtggtatta aaacttttag agatggtgat
    31081 ggcttatggg aaaaccatcg cattgaagaa gtggcaacct atgatgcttg gttaaataat
    31141 cgcgatttgg ttttagaatt ttacaatcaa cgtcgaaagc aattattgac ttgtgaaccc
    31201 aatgatgctc atcgacaatt ggtaatatta gaagaaaaat ttaatgtaca aattatcact
    31261 caaaatgtcg atgatttaca tgaacgcgca ggctcgaaaa acgtattaca tttacacggt
    31321 gaattattaa aagtaagaag cactgctgat gaacgattag tttacgattg gaaaatcgat
    31381 ttagtaaatg gtgataaatg tgaaaaacaa tcgcaattgc gaccgcatat tgtatggttt
    31441 ggtgagcctg ttcctatgat ggatgcagca gctaaattag ccgctaaagc tgatattttt
    31501 attgtaatcg gtacttctct agtcgtttat ccagcagcag gcttgttaca ctatacagac
    31561 tatacagtac caaaatatgt tattgaccca aacagacctg ttgtttcgaa tattcaacca
    31621 atagaataca ttacagaaaa agcaagcatt ggcgttcgca agcttgttga acgactcatg
    31681 gttgaataat cttatttttt tagatagaaa gcatgaccag caacaccagt attgccattt
    31741 ttgtctacac ctaacacatt gacataagct gttgtatctg cttgaagttc aaaaaatatt
    31801 tttttagaaa aattaaaatc agcacttgtt acatttgctt cctctttgta ccatattttc
    31861 ttagtgtact gattgaacaa aataatttta taagattgaa gatttttgtc ttttatatta
    31921 aagtgtaaaa aaatcgaatc ttgcataaac acagtgtctg caataatagg tgatacaatg
    31981 tttatgacag gagcacttgt ttcccatttt ttacaactta agaaaaacag acagacaaaa
    32041 cagtaaaata ttttatttgg catttaaaaa atatattttc aaagttagct aaaaaaacta
    32101 tctttaaatt attcaaaaga aacaagctat ataaaacatg caaaatttag tgaaaattga
    32161 aatcaactat atatgctata caagttggca tgaggtgcca gaaaaatatc agcatttgat
    32221 ggaagaagct aaaaaagcaa aagaaggaag ttatgcgcct tattctaact ttaatgtagg
    32281 tgctgcgtta ttattgaaca atgggaaaat aattcatgga agcaatcaag agaatgcctc
    32341 ctatcctatc ggattttgtg cagaacgaac ggcattgagt gctgcagcat cacttgcatc
    32401 gcacgaaaaa ataagtgcca ttgctatcac ttgtagtagc atcaaaaatc ctgtaacgaa
    32461 gccagctgct ccttgtggca tttgtcggca aacaattttt gaagcggaat gtaaacacca
    32521 tcaaaatatc gaaataatat taatgggcga aagtggcgaa atatttcatt ttaaatctat
    32581 aaaagactta ttgccattgc attttgatgc tgattttttg tagtaaattt ctttataatt
    32641 ttgctggaca agcatacgat ttattcgtac caaattcttg attgatgaca attattttgt
    32701 ccactgtttt tgctgctttt gctatacaaa ttattattcc tttacttgtt ttactttcac
    32761 caggtttata agtatcatta atcattgctt gtgttgattt aaaaactggc gtcaactttt
    32821 ctggttgagc aggttccatt tttggataga tagagccaaa cgacaatgga tactgtgata
    32881 aaaatgcttt cgatccggtt tctgctgcat aaaattttaa agaattgaat aatgcttctg
    32941 aagcagctgc gctcaatttc acatcagcgc ttgttgtatt cgttgccttg atttctactt
    33001 ctgccaactc gtatgcaaat cgctgtgaaa tatcatcagg aatagtaaca gccacaccac
    33061 gaggaattgg aaatcgtgtt agtttaacaa tttgcacttt cataccatta ttcaacagca
    33121 cttcattagt tgtattttgg aaagtatgat cttttgagtc tatagtaaag ttgacaaatt
    33181 gcaatgaatc gacttttcgt ttttctgctt ttggatcttt tgttggtgtt ggtggcactt
    33241 ttggatttcc tcctttaacg gctgcaattc caatgataaa tgaaagcaat aaagcgatag
    33301 gtagcaataa ttttttcatg tcaatatttt tatactttac aaagcaagtt acaaaaaata
    33361 gcgaattact attattatct ttgtaaacaa acaaaatgca tgcctaaaat tttcactttc
    33421 ttattcttat tttttgtgac atatcttgga ttttctcaag aaaatgaaat catcaaacaa
    33481 cttgatttgc taacacaaca agccaaagga aaattatgtg tctatgcata tcactttgaa
    33541 gacagcgcat ctataaatta ttatggtgat gaaatgtgtg tgcagcaaag tgtttttaaa
    33601 tttccaatag ccttagctgt tttagagaaa atagacaaag gcgatttttc attacaacat
    33661 aaaattcaca ttactgcaaa agacatgcaa caggaaactt ggagtccgct acgtgactcg
    33721 tttccaaacg gcaatatcaa tgttacattt gaaaacctat tgaaatatat ggtatcgcag
    33781 agtgataata tcgcatgcga tgtattaatt caccatcttg gcaaggcaaa aagtattgaa
    33841 aagtttgtac gtaaatttga tattgatgat ataaaaataa gatacaacga agaaaagatg
    33901 cagaaaaact ggaaaaatca gtataaaaat attggcacac caaaagcata tgtacaatta
    33961 ttgcaacaat tttatgatgg caaaattatt tccaaaccaa gcacagattt tctatacaaa
    34021 gtaatggtgg aaacgacaac gggaaaaaat agaattgtga atttgctgcc tgaaaattgt
    34081 atcgttgcgc ataaaacagg atcatctggc acgaataaag acggcatgac agcagctacc
    34141 aatgatgttg gcataatcac tttaccaaac ggcaaacaca ttggtattgc tatctttgtg
    34201 caagatgcat ttgcagcata taatattatt gaaaaaacaa ttgcagaaac ggcattgctg
    34261 attgtaaatc attacaataa ataattttcg aatttcgata aattactcat cgagtaattt
    34321 aatgtctata ttttcggtac ctgcaattcc ttctacagaa ccagaaatat gagaagcatt
    34381 caataagatt ttgtctaatt gtttttcacg tgctttccat aatttttcca tttgcgttcg
    34441 ttctgtatca attgaatggc gcattgccaa aaagccttcc cgcactgctt tccactgttc
    34501 gccgaattca ttactcgtta aataatcata gagcaattgc attttatcgc ctttattttc
    34561 ttgcgatttg ctcacggcaa atacttttaa aattgtacta cgcagcacat gtacgagtgc
    34621 gcgcacttcg ctaaacgtac aaatccaaat gccatttttt tcaccaaact gttccatatc
    34681 ttttggcatt gtttgtgtca ctatcacagc aatatcagca gttatcgcgc gcatatctgc
    34741 ttttagtttt tcaatccaat cgccaccaaa tgcttgagtg cgcttgcttt caaacaaaat
    34801 ttttccgcaa acctgaccaa aattattgcg cactgtctgc acacaatcgg cacctttgga
    34861 gcctttccca acctcttcga taaggtcaaa tgggaatgct tcttttagca atgactctaa
    34921 cgccaattct tgcacttctc cttgtagctg catgcttccc tgcgatgctt tgcgctgtgc
    34981 ttcttctaat gctttttgtg tttgttctaa ttttttttca tattctctaa agcgcaagtc
    35041 atttttttct tgctcttgtt tggctatttt ttcttttaat agttcccgtt cttcattcaa
    35101 tttacgttgc aattgtaatt ctatttcttg ttctttctct tttaattcat tttcacgttt
    35161 taagaagtct aattcttttt gttttaatgc aacattttct tgtttgcgtt tttctaattc
    35221 ttccgtttgt gattttagtt gctgttcaaa ttcttcttta attttcttgc cttgctcagc
    35281 actaattctt actttttctt cttctatttt ttggtctaat cgtttttgaa aaagctcatt
    35341 ttcttttgct ttcttatctt caaaatcttt ttgctgtaag gctaatgctt ccttttgttt
    35401 tacaaattca acatttgcta attggagttt ttcatcaaat gcttttttat atttatcttc
    35461 tgcttgttgg ttgagcacaa cgtcaatatc tattgatgta ttacagtttg gacactttat
    35521 tttgttatca ctcattttaa taatttttct attctaaaaa tatatcgata aaaatacaat
    35581 aattccgata atccaactta aagaaatcaa tagtaaatag ggcaagaaag taatgtcttt
    35641 tattttatgt ctgaagataa tcatcgacat acaagcacct acaaaaccac ctaccagcgc
    35701 gagtattaga agcgctcgtt ctgaaatcct ttgtgattta gtcatggcaa aaagtttatc
    35761 taaggcataa gctataaaac tgatggcatt aatggtgatt agatatgcac aagtataagt
    35821 ttctatgctc attttttgtt agcttaaaac tgttaaaata gtaataaaca atcacaaatt
    35881 agtttaaatt tgcaccgctt ttgcattttg taaaacaata agaatggcag aaaaacaaat
    35941 tcacggatta cacaaactat cagcagctgg tgttttgata tccttaggca ttatttttgg
    36001 agacattgga acgtcgccaa tatacgttct aaaagctgtg atgaacatgg caaccggcgg
    36061 caaccacaaa ataacagaag accttgtatt aggagctgtc tcttgtgtat tttggacact
    36121 tactattgtt gttacgatta aatatgtcat tttggcttta aatgcggaca acaaaggtga
    36181 aggtggaatt tttgctttat atgcaattgt cagaagatac aaggctaaat ggacaattat
    36241 acctgcatta atcggatgtg cttcgttgat ggcagatgga tttataacac caccaatttc
    36301 catttcgtct gcggttgaag gtttgacaat cttatatcca ggaattcaaa ctgtgcccat
    36361 tgttatcacc attttagctg gtatattttt agcacaacag tttggaacta atagaattgg
    36421 ctctttattt ggaccaatca tgtttgtgtg gttttcgatg attggaacat taggtttttt
    36481 tcaaattatt caaaatcctt ctgtaattaa agcgctcaat ccaatgtatg ccatcaattt
    36541 ggtgttgaat tatcctggtg gattttggtt tttaggagcc gtatttttat gtagtacagg
    36601 tggtgaagca ttgtattcag acttaggtca ttgcggtaaa caaaacatta gagtaagctg
    36661 gactttcgtg aaaattgctt tagtattaag ctatctcgga caagctgcat ttatcatcaa
    36721 tcataaagat tttgactatg aaaatattgc accattttat gcagtaatgc cagaatggtt
    36781 tttaaaaatt ggaattgtca ttgccactat ggccactatc attgcatcac aagccttaat
    36841 cactggctgt tttactttaa tcaatgaagc gatgaaatta aaattatggc ccaatcaaaa
    36901 aattatttat ccgagtatag tacaaggtca aatatatttt ccttttatca attggatgct
    36961 gtttttaggt tgtgtagctg tagtgctcat atttagagaa tcaggaaaaa tggaagctgc
    37021 gtatggtctt gctatttcat taaacatgat tatgaccacc agcttattgg tgtactatat
    37081 gtatgtaaaa cgtaaaccga aatattttat ttatggtttt attgcagtgt atgtttggat
    37141 agaatttacc tatttagtcg ccaacttaag taagttctcg catggtggct ggtttgctgt
    37201 gttaatcgca gttgtgattt ttgtattaat gtatttatac aacgaaggaa aaaaattacg
    37261 taaaaaacat atcgaatttg ttgaaattaa agacttctta gacgatatca cagatttgca
    37321 aaacgacact acaattccga aagaggcaac aaacctcgtg tttatgtgta atgcaaatga
    37381 caaaaaacat atcgactcaa atatcattta ttctatattt cgaaaaaaac caaaacgtgc
    37441 tgatacgtat tggttagttc atgttgatat tacgaatgag ccttatggcg caagttattc
    37501 tgtcgatacg attattccta aaaaatgctt ttttatacgt ctaaaatttg gctttaaagt
    37561 agagcataaa gtacatgtca tgtttacaaa aatagtagaa gatatggaaa gccgtggtga
    37621 gattgattta atgagccatt atccttcatt gcgcaagcat ggctatccgg cagactttaa
    37681 atatgtaatc atgaatccat tagtatctat cgataataaa cttaatccat tcgaacaatt
    37741 tttgataaaa tcttacaatt ggtttaagtc aattagttta tctacacaag aagattttgg
    37801 tttggaaaaa acaaactgcc gtgtagaaaa tgtgcctatt cgtattgcgc aaaaaacaca
    37861 tattgaaata agaagagaag actaataaaa gaaaatttat ttttatttgt taagcaattt
    37921 ctggttttaa attgttggtg aatgtaaata caatcagcat cacaaaaacg atcaacacgt
    37981 ttgtagaaaa aagttcgctt gaaaatccag gatttacaaa ggtcgctatt cctattataa
    38041 agctacttac agcagccatt gctatcgttt tataaaacaa gtttcctttt ataaaataag
    38101 tagcaaacac tacaaacgaa caagcaattg cactccattt tatccaggtg aatatgtgta
    38161 ataagctcaa atgaacacca tacaaagcat aattatcaat tggattgata gcatttaagt
    38221 cgatgtttct ttttataatt aattgatgga tttggctatt ttctaaggca tcgccaagca
    38281 acatcaacac tgacataagt attgcaatat tcataagctt tgaagaagta ctttttttga
    38341 taaacatggc aatgcaaaaa ataaaactac tgtataagca cataaataaa tagtcaatct
    38401 tattgcctaa tagcatttgt tgctcgtatt gttctggatt ttgaacagta aaatattgca
    38461 gaacagcctc tttgctgtta ataaattcga aggcaataat tggtgtttta aagccaggca
    38521 ttaagttgtt ttctgctttc ggattggtaa ataacaaaac cactccagta atgatgatgg
    38581 caacgccaat aaatcttgca attttcagat atttcatttt ttttagattt tataaaaaat
    38641 attgatatta atcaatacat caataaaatg tttgtcataa ctttaattta acaaacataa
    38701 ataccctacg aaaaagaaaa tggctgccta agcagccatt tttttattgt ttctcgcgtt
    38761 tatttaattc atcgcgtatt gttgctgctt tctcataatt ttcatttgct aatgcttcat
    38821 caagcatctt cgataattct ttataggtgt aaattgtgta atctgtttta tctttttctg
    38881 agacactcga tacggcatct aaaataacat cttctatttc atcaatttca gggtttgttt
    38941 cttccatcaa gattaatcca gaattatcta ggatatttcc ataaatataa attgggcaat
    39001 taaatctgat tgctaaggca attgcatctg atgttctaga gtcaatttca tattcttcgc
    39061 cattaaatat gcaaattaat ttggaataga aaacgccgtc caaaagattg gtgattataa
    39121 tttcgctaag cgttacatga aatgtatcca ttactgtttt aaacaaatca tgcgtcaatg
    39181 gtcttgccgg cggcatattt tccattgcaa ttgcaattgc ttgcgcttca gtgcctccta
    39241 ttacaatcgg caaccttcgt ttgcctttaa tttcgcctaa cacgactgca taagaatggt
    39301 gatgcgtaaa accttgtgta atattaataa tttctaattc aatctttttc acgaagtaaa
    39361 tatactaaaa acgaagtttt acttattaaa aatttttcta caattaaaca aaaaagccac
    39421 caaaatcttg gtggctttta tctaagtact aaatactaag tactcaatag tattctaatg
    39481 ttgactagct ttcaatgctt taactgcctc cgttaatttc ggtacaattt cgaatgcatc
    39541 tccaacgatt ccataatcag cagctttgaa aaacggtgct tctggatctt tattgatgac
    39601 aacgatacat tttgattggt ttacaccagc caaatgttga atagcacctg aaataccaat
    39661 agcaatatat aagtttggac gaatagcaac acctgtttgt ccaacgtgtt cgtgatgagg
    39721 tctccaatga acatcggcaa ccggacgaga acaagccgtt gttgcgccta aggcacttgc
    39781 taagtcttcg ataattcccc aattttctgg acctttcata ccacgaccag cagacactac
    39841 tagttctgct tctggtaatg gagcaacatc acctgtttgg cgtgtcactt cttttacggt
    39901 aatgcgactt gctgctggtg ccacagtaga tgcctcaact gttgcagtac cttcgccttt
    39961 tgttgcacca aatgaatttg gcactacagc aacaacctta acagctgtat ttacttttac
    40021 aaaagcaaat gctttaccag agaaagctgc ttttttcact acaaaatcag catctggcaa
    40081 accaacggca ccagaaacga taccagcttt taagcgagct gaaaccactg gcgcaactgc
    40141 ttttcctgtt aaagtttgtg agataacaat tgtatctgca tttacttgag tagctacttc
    40201 tgccactgct tttgcataag cacttgcatc aaaattatca aatcctgcgt tatttgcatg
    40261 aatgattttt tttgcaccat atattcctaa tgcacttaat gatgcatcat caattgttcc
    40321 taatgctaaa cctacagcgt ctttacctgt ttgttttgca tatgcagtag cataagaagc
    40381 agcttcgaag cttgcttttt ttactttacc atctgctatt tctactaaaa ataaaattgc
    40441 cataattata tcatttttgc ttcttcgtgt aacaatcgga ttaattcttc aggttgatct
    40501 gctgagatca ttttacaagc acctttagct ggtggtaatt catatttcac cactgatgtt
    40561 aatgatgctg caccttgtgg cgccacaact gccaaaggtt tagttttagc actcataatt
    40621 cctttcatat ttggaatacg agcttctgcc atacctttcg cacatgaaat tacgaatgga
    40681 gtatcaacgc ttaaaacttc tttaccacct tcgatttcac gttcaatagt agctgtatta
    40741 cctgccatat ctaatttaga tgcgtaagaa acaaaaggta agtcgattaa ctctgctaac
    40801 ataccaccta aatttgaacc attatagttg attgtttctt tacctaataa aactacatcg
    40861 taagcacctg attttgcaac agctgcaatt tctgaagcaa ttgcaaatga atcagcatca
    40921 cctacaacat caattctaat tgcatcatca gcgccaattg ctaaggcttt acggattact
    40981 tggtcgtttt ctgctggacc aacgtttacc accgttacgg taccgccatt tgcttctttt
    41041 aattctaaag ctcgaactaa ggcataccat tcatcgtaag ggttcaagat ccactgtact
    41101 ttatcgttgt tgaattttgt atcattatca acaaacgaaa ttttagtagt agtatcagga
    41161 actaaactta tacatactaa gaatttcata aatttgttat ttagtttaaa aatttgcgat
    41221 gctaatttac tcattttaat taattcaatg catgcataca aatagaatac atcgattaga
    41281 atctatgatt gttgaaaacc ctagagatac ctttgcgtta tttgcattgg caaaagaata
    41341 tgaaaagaca aataattaca taaaaagtgt ggaactattt gaaagactgt tagttataga
    41401 cagcaattac cttggtgcat attatcattt agcgaaaact tatgaacaaa tcggcgaggt
    41461 taaaaaagca ttaaatattt atgaaactgg aatttcgtta gcaatagaac agaataatac
    41521 acatacatta agtgaattaa ggaatgcaaa aatgaatttg gaaattgaat tgtcataaat
    41581 tgtattaatt taaatacctt aaagactgat agatagaaaa tttcaagtaa ggaaagtatt
    41641 agaacctatt gcaaaattaa tttgcaaaaa ttaaaatcat ttttattatc gtatttttga
    41701 agcatgttac tacgtattca agaaaatatc atacacaata atctcgcaaa tactggcaca
    41761 cccatcttgg tggcttgtag tggcggcatt gattcaatgg tgctttgtga tgttttacta
    41821 cgtttgaatt tcaaaattgc aattgcacat tgcaatttcc aattacgtgg aaaagaaagt
    41881 aatgcagatg aaaaatttgt tgcacaattt gcaaaacaac atcagttaga atttcattcg
    41941 ataaagtttg atacaactaa ttataaaaaa aataattcca tttcaacgca aatggctgct
    42001 cgcgaattgc gatacaattg gtttgaacag ataagaaaag aaaataaata tcacagcata
    42061 gccacagctc accatttaga tgaccaagta gaaacactcc tattgaacat cacaaaaggt
    42121 gctggcataa aaggtttaac aggcattcca gcaaaaaatg gatttattat tcgaccgctt
    42181 ttgaaaatta gcaaacagga aatattggat tatgcttcat tgaacaaaat tgcatttcgg
    42241 gaagacagtt caaatgctag tgatgattat cagcgcaatt taattcgtca tcagattaca
    42301 ccatatttag aaaaaattaa tccatcattt cacaattcca ttgatgagtt tattggcaga
    42361 atgaatgatt atcaagcttt gacaaatcag caaattgaaa gtataaaaaa gaaatgctta
    42421 agagaaaaaa atggaattat tgaaataaaa ttaggttata taaaagcaca ctttgcaggt
    42481 gctactgtct tatttcatct aataaaagaa ttcggattta atattgatgt cgttcaaaat
    42541 atattaaaag taaaagaaag cggtaagcaa tttttttcag agacacatag aattatcata
    42601 gacaggaaaa acttgtttat tgctacaaaa aatccagaaa gagatagcat tattatatta
    42661 gaaaaaattc catcacaaat tattttcaat aattataaga ttcaatgtac atcaattccg
    42721 atacaagaag taaacataaa aacttcaaat cgatatgctt attttgatgc tgacaaaatt
    42781 gaattgccct taataattcg ctatgctaaa gaagctgatt atttttatcc atttggatta
    42841 agcaagccca aaacaccagg gaaagcaggc aaaaaaaagc tctctaaata tttcaaagac
    42901 gaaaaattca cattactcga aaaagaaaat acgcctatta tattttcagg cgagaaagta
    42961 atttatttag tcaagcacag aattgatgac agattcaaaa tttctgaaaa gaccaaaact
    43021 gttttaaaaa tggtcattat agacgattct atctaataca cttagtacaa catcaagcaa
    43081 tgtacattat tactttttaa aacattcaaa agcaatatct aatatttcac agtgaataaa
    43141 aatgtgtaaa ttagattatg caaatattta actacggtat tgatacatta agtgttgcaa
    43201 aagcattagc attagcaaat ggtaacgcaa aaggagttat aaatgatgct gctaaagaga
    43261 aaatcaaaac atcacagcaa aacgtacagc aaattgtaga aaaccacgat acggtgtacg
    43321 gaatcaatac aggtttcgga attttatcca acaaaaaaat atctgaagaa gatacacaaa
    43381 cattgcaata taaaattttg caatcacata gtgttggagt tggaaatgcc attcctaatc
    43441 acattgcaaa gttaatgctt atcacaaaat tacaatcttt agcacaagga ttttctggtg
    43501 ttcaactaag tactttagag agaattttat ggcacatcga taatgatatc attccaattg
    43561 taccagaaaa aggcagtgtc ggcgcatctg gcgatttggc accactatcg catttgtttt
    43621 tacctctaat tggcttaggt gaagtaatgt ataaaggcaa aactgttaaa acttcaatgc
    43681 ttttaaaaca agaaaatctt agcccaatta cactaggccc aaaagaaggt ttggcattaa
    43741 ttaatggtac tcaatttatt ttatcatttg ccgtcgcagc aattcagcgc atgcataatt
    43801 gcctcgaagt tgccgatatt attggtgcaa tgagcttaga agctttgcaa ggcaccaaag
    43861 caccatttga tgaacgttta catcaactac gaccttttaa aggaaatcaa ttagtagcac
    43921 aacgtttgcg tttgttatta cataattctg aaattatgca aaaccatgaa gattgcgagc
    43981 gtgtgcaaga tccatatagt ttgcgttgta tgccgcaagt tcacggtgca tctagaaatg
    44041 catggttaca tttaaaagag ttgactgaaa tcgagttaaa tgctgttacc gataatccga
    44101 ttattttcaa cgcagatgat accataagcg gtggaaattt tcatggtcaa ccattg
//
LOCUS       DAOQQV010000006        42481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_248_length_42481_cov_7.811649, whole genome shotgun sequence.
ACCESSION   DAOQQV010000006 DAOQQV010000000
VERSION     DAOQQV010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 42481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42481
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_248_length_42481_cov_7.811649"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1282)
                     /locus_tag="PLL59_01335"
     CDS             complement(77..1282)
                     /locus_tag="PLL59_01335"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006090.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HMY22472.1"
                     /translation="MAEAYIYDCVRTPRGKGKKNGALHEVTPTELLAQMLKALQQRNN
                     LDTTLVEDVIVGCVTPIGDQGGNIAKTAVMMADYATTVPGFQLNRFCASGLEAVNLAA
                     MKVRSGWQDLIIAGGVESMSRVPMGYDGGAWVLDMRVNNHTDFVPQGISADLIATIEG
                     FSRTDCDNYALRSQKLAGIAKAEGRFKKSIVPIVDINGLAILEEDEYNRPDTTLEGLS
                     SLTPSFQMMGEMGFDNTALSKYTTLEKINHIHTPGNSSGIVDGASLVLIGSKEIGEKL
                     GLKPRAIIRSVAVTSTEPTIMLTGPGPASKIALQKAGMTINDIDLVEMNEAFASAVLR
                     LQRDINVPDEKLNVNGGAIALGHPLGATGAMILGTLVDELERTNKSTGLATLCVGGGM
                     GIATIVELV"
     gene            1651..2817
                     /gene="purT"
                     /locus_tag="PLL59_01340"
     CDS             1651..2817
                     /gene="purT"
                     /locus_tag="PLL59_01340"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HMY22473.1"
                     /translation="MKKILLLGSGELGKELTIALKRLGQYVIAVDSYNDAPAQQLADE
                     REVINMLDGEALDQIVAKHQPDLIVPEIEAIRTERFYDYEKQGFTVVPSAKAANFTMN
                     RKAIRDLAAKELGIKTAKYEYATSLEELIAATIRIGMPCVVKPLMSSSGKGQSVIKTK
                     EDIEKAWSYAQAGKRGDQIEVIIESFVDFYAEITLLTVTQKNGNTLFCPPIGHRQERG
                     DYQESWQPAYVKIEDLLEAERMAAVVTQALSGAGIWGVEFFLTNNGVYFSELSPRPHD
                     TGMVTLAGTQNFSEFELHARAILGYPIPEITLERVGASAVILSESDGNNPRYFGVEDA
                     IKQTKSDVRIFGKPIMRPYRRMAVALAYDVLGSDVEIVKNRAIEIANKISVKNI"
     gene            2871..3350
                     /locus_tag="PLL59_01345"
     CDS             2871..3350
                     /locus_tag="PLL59_01345"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HMY22474.1"
                     /translation="MIYNAYFKSPIGILEIKSSATHILSIEFVEESDFEKQQINDLIH
                     DCILQLTAYFSHLLHQFELPLAFNGSAFQNKVWQELQQIPFSTTCSYLQFAKKLGDAK
                     CIRAAAAANARNPFAIVVPCHRVIGSNGKLVGYAGGLWRKQWLLEHEQAISLQTKLF"
     gene            3458..4660
                     /locus_tag="PLL59_01350"
     CDS             3458..4660
                     /locus_tag="PLL59_01350"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HMY22475.1"
                     /translation="MGKRVLIIGAGGVGNVVVRKCASVPEVFSEIMLASRTKSKCDAI
                     AADIKHMNIKTAALDADNVPEIVALIKEFKPDLVMNIALPYQDLTIMDACLETGVHYL
                     DTANYEPKDEAKFEYSWQWAYQERFKEKGIMALLGCGFDPGQSQIYVAHAAKHHFDEI
                     HYLDIVDCNAGDHGQAFATNFNPEINIREITQDGKYWEDGEWKIIPAMSIHKAIDYPN
                     IGPKESYLLYHEELESLVKNFPTIKRARFWMTFGAQYLKHLEVMQNIGITSIKPIMFQ
                     GREIIPIEFLKALLPEPGSLGENYSGETSIGCQIKGIKDGKDKTYFIWNNCKHQDAYN
                     DAKAQGVSYTTGVPAMLGAKLMLEGIWMKPGVYNTEEMDPDPFMSQIGGYGLPWHEEI
                     NPVLPHEY"
     gene            4771..6372
                     /gene="ade"
                     /locus_tag="PLL59_01355"
     CDS             4771..6372
                     /gene="ade"
                     /locus_tag="PLL59_01355"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HMY22476.1"
                     /translation="MQIKSNYIDIFNQQIFAAEISIENGKIKSIVKINEAVETYILPG
                     FVDAHVHIESSMLVPTEFAKIAVIHGTVATVSDPHEIANVLGKAGVYYMLENAKKSPL
                     KFYFGAPSCVPATTFETAGAVVNADDIDELMSLDDIKYLAEMMNYPGVLHRDKLVMDK
                     ITIAQKHNKPIDGHAPGLTGHEALQYISAGITTDHECFTFEEAKFKLEHGMKVLIREG
                     SAAKNFNALIDLLHEYPDDMMFCSDDKHPDDLLFGHINDLVKRAVNKGVSLFNVLKAA
                     CVNPVLHYQLNVGLLRVGDEADFIRINNLEDFSIVETYIGGHCVAKNKQDFIETKPEK
                     IVNNFSVKTKQKEDFNLLVKQEKVRVIEAIDGQLITQEYIGKIVVDNDCYAHANIDDD
                     ILKMVVVNRYHDAPIAVAFVKNFGLKNGAIASSVAHDSHNIIAVGVNDDCICRAVNTI
                     IAAQGGLAVVDEENCDILPLPVAGLMSNMSCIAVAEKYTTLVNKSKSLGCTLHSPFMT
                     LSFMALLVIPNLKLSDKGLFDAIRF"
     gene            6426..7550
                     /gene="nspC"
                     /locus_tag="PLL59_01360"
     CDS             6426..7550
                     /gene="nspC"
                     /locus_tag="PLL59_01360"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HMY22477.1"
                     /translation="MTYDHIPSPSFVLEENKLIKNLQLLKSIQDEADVHIICALKGFS
                     FHAVFPTVKKYIHGATSSSLHEAKLVYEKMNVRAHTYCPVYIPKEFDEIQQISSHITF
                     NSLSQYNTYKHKMIPDISYGLRVNPGYSEITTELYNPASPNSRLGIARNLIGDVLPAG
                     IEGLHFHALCENTSYTLERVLESFIKLYHPLIQQAKWVNFGGGHLITRKDYEPQHLIQ
                     ILKVFKSKYPNLQEIILEPGSAIGWQTGVLVSTVLDIVPNGNIPTAMLDVSFTCHMPD
                     CLEMPYKPNVLSAVENGTFEYNLGGMSCLAGDFIGTWKFENELKIGDRIVFDDMIHYT
                     MVKTSTFNGINLPSIGVMNENGKFELIRSFSYADYKNRLS"
     gene            7568..7951
                     /locus_tag="PLL59_01365"
     CDS             7568..7951
                     /locus_tag="PLL59_01365"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HMY22478.1"
                     /translation="MQKILDNLYRQAERIISSDTLVSKLIDTVFLKLGEASESFYKIQ
                     DNIIALTRMLRAWVKGDYKNISTASIIAVVAALLYFINPLDIIPDFIPIIGQLDDIFV
                     LSYLIKTLNKEIERFMAWEEEQASH"
     gene            complement(7943..8596)
                     /locus_tag="PLL59_01370"
     CDS             complement(7943..8596)
                     /locus_tag="PLL59_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMY22479.1"
                     /translation="MQEINVFVVDDHQIFLDGIVSLLDDEPNIKIVGTAHNGKQAIDR
                     IKTQKVDVVLMDINMPEMDGIEATKQLKKINPNIKILMLTMHSEPRFIKECLEIGAKG
                     YVLKNISKDDLLKAIDSVYQDKSFLDQEAQEKLINSISNSDEVDDRNYDELAAQITQR
                     ELEILQLISLGLTSQDIATKLFISKNTVETHRKNMLAKLNVNNTAALLKIAYKKGLV"
     gene            complement(8654..8995)
                     /locus_tag="PLL59_01375"
     CDS             complement(8654..8995)
                     /locus_tag="PLL59_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22480.1"
                     /translation="MWWIIISPNIIKYFTPKKIAGITLFPFIIVEKKDLKYDKTFINH
                     ELIHIYQQMELFVILFVILYYIEFFILLLKYKNVTQAYRNISFEREAYENETNYNYLE
                     TRKWFSFIKYW"
     gene            complement(9035..9712)
                     /locus_tag="PLL59_01380"
     CDS             complement(9035..9712)
                     /locus_tag="PLL59_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMY22481.1"
                     /translation="MQSLISYFFLLTLKYWSKLFYTIQITWPSTPINWDNTKLILLLN
                     HTTLYEFIFLGFLPNHFLRKLSKRMVAPGADKSLNRPLVGIFFKLFSPGMISITRKRD
                     DSWDNFLESIYEDSIILIAPEGRMKRKNGLDLYGNKMTVKSGVVDILNGLDKGEMVIA
                     YSGGLHHVQVPGEGLPKLFKTVKLHLESLDIPSYKKMFNTTIGSEQWKQAVLADLQWR
                     LENKVPV"
     gene            complement(9879..11132)
                     /locus_tag="PLL59_01385"
     CDS             complement(9879..11132)
                     /locus_tag="PLL59_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMY22482.1"
                     /translation="MSTPKKDPYIALRYKEFRAYVLARFLITIAVTMQAIIIGYEIYE
                     LTNSKLLLGFIGLAEAIPAIGNALYGGYIADKSDKRTMLVMFVGIYTILCATLLFFTH
                     TSMIASIGKDNVVIIIFVIIFLTGIARSFSGPASFGLAAQIVPRDVYQSAITWSSSAW
                     QLGAVLGPGIGGLLLGILGITHTLSIIVIILSIAVLAILYIEKKPVQFVPQNESVVES
                     ALQGLKFVFHNKVILSCITLDLFAVLFGGAIALLPVYAKDILHVGAEGLGFLRAAQAI
                     GAIIMLMYLAYFPIKKNAGIILLLAVFGFGVFIILFGISKIFWFSMFCLMMSGALDGI
                     SVSIRHTIVQLSTPDEMRGRVSAVNSMFIGSSNEIGEFESGATAAAMGTVPAVIFGGC
                     MTCLVVIVTYFISPSIRKMELNDAT"
     gene            complement(11161..12015)
                     /locus_tag="PLL59_01390"
     CDS             complement(11161..12015)
                     /locus_tag="PLL59_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HMY22483.1"
                     /translation="MSKIINLTSIPSAATLYAGIATTFIKKGKGDLPDIHVHLDKAVI
                     QASEMSKYNKVCGFDNQRTVSSTYLHTFVFPLQMKLLSQPEVPFPLAGLVHFANSIKQ
                     YRPLYVGEEFSATCKLGNLIAHEKGQAFEIISIVDVNGTRIWEDTSIYLYKGKEGIGS
                     VLEWHQPELSANSIKSTWSLPTNLGFEYAKASGDFNPIHLHSLSAKLFGFPGHIIHGM
                     WNLGKIVSEFESRVPENYELTVLFKLPIFLPANVIFRHEKTNLGFDFDIVDKTQEKPH
                     LKGYMNKL"
     gene            complement(12156..12566)
                     /locus_tag="PLL59_01395"
     CDS             complement(12156..12566)
                     /locus_tag="PLL59_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22484.1"
                     /translation="MFFVKVLLISILSAVSTMFLPWYIPFVICFIIGFILSNRPGNNF
                     IAGFIGVGLFWLIYALFLDIKNQHVLSAKVAQLFSDSLQTSFTGAVLIMITTFLGALL
                     GGLSTMAGAMIVDDGSRKRLRKAVKSGRYTLKMK"
     gene            complement(12577..15012)
                     /locus_tag="PLL59_01400"
     CDS             complement(12577..15012)
                     /locus_tag="PLL59_01400"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMY22485.1"
                     /translation="MIKKVSGILIAIIVAIGIFFLNKPMGELPALGQFFSPTHGFWQN
                     ASTDNNKTSSIVIKCINGEATVVYDEYNIPHIFADNEDDLVYAQGYVEAKDRLWQMEF
                     QTHAAAGRLTEILGNRIISGHSVLDADRFSRRIGILRAAHNSVKEMEKDPISKKIMES
                     FSAGVNAYIKQLKKADYPIEYKLLGYQPEAWSPLKCALLMKYMAKDLTYYDTDIENTN
                     ALRAFGEEVYKKMYPDFPPPALDPIIPVNTKWNFTSVDTFSSKNTATTKTALLYSNPQ
                     KALYANKFYGSNNWCINGNKTKSGKPILCGDPHLALNLPSIWYAIQLHCPTINVEGVT
                     IPGAPGIVIGHNDSIAWSVTNSERDVINNFAVEYSADKKSYKLGNAYVPFEYDVQVFK
                     IKGSKDVVDSIRMTKVGSIVYDENYGETNDKKHIATYWRAEEPSNELMTFYYLNKAKN
                     HQDYLAALDYFSCPGQNFIYADAQNNIAIKQQGNFMLRTQFTDGIFVTPLANVDITKL
                     KARIPNAQNPHILNPERNFCSSANQNPVDNNYPYSTSGHYENYRNRVINSVLTSTVQS
                     SWKDMAALQSNNLSLLAKETLPTLLATIVSNNSETSNQILRALKSWNYEANINLTAPS
                     YFYKLWSYIEETTYDELLKTKSSFRLPENYTTANLILHEPDFVLFDIKNTANIEKAQD
                     VVQLAFQKTVAFFEDYAKNKDKNLQWSHYKNTTVTHLARIPAFSVSDIPIGGYNNIVN
                     ATSEGAGPSWRMIVDFAQGKPTCYGIYPGGQSGNVGSIKSTNYIDKWSKGEYNNLHFF
                     ANKEEALKIINKQ"
     gene            15104..16717
                     /locus_tag="PLL59_01405"
     CDS             15104..16717
                     /locus_tag="PLL59_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMY22486.1"
                     /translation="MTNNEIAKHFSLLADLMELHNENAFKIKSYAFAGRTLNKRNEAL
                     STLTLDELENVEGIGKAIAAKILQLTQTGKLDLLENYLAKTPIGVVEMLQLKGIGPKK
                     IAQLWKELEIESIGELEYACQENRLVELKGFGTKTQENILQQIQFLQQNAHKFLWANL
                     EELANEILAEIQNAYPEILVSTCGDYRRKSIIVERVDFIIATTQNEIQEALVEKYKQY
                     PVHFYFCDTKHFYWNLLQLSSDKEHAGFLSFKIDTNKSFTSEKEIYVEAGFPFIAPEL
                     RDNKYEWKLIEENNLHHLVELQDIKGIVHTHTKFSDGANSLAEMASYCKEQGFEYVVI
                     SDHSKSAFYANGMQEERIFQQHREIDKLNEQLAPFKIFKSIESDILYDGNLDYDDDIL
                     STFDLIIASVHSQLKMNEERAMLRLIKAIENPYTTILGHLTGRLLLSRNGYPIDHQKI
                     IDACAANKVSIELNANPYRLDIDYKWIAYCQEKNVPICINPDAHNLRGVHDIRYGVFA
                     ARKGGLLKANTLNTLTKDNFEKYLSHTKR"
     gene            16753..17292
                     /locus_tag="PLL59_01410"
     CDS             16753..17292
                     /locus_tag="PLL59_01410"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMY22487.1"
                     /translation="MKKCFLFLVFIAFASFVFVQCKFKDDKNEIIVETFKHDSILANN
                     PVLKKADSMAKITAQTEEERLKTVTSIKFEKEVYDFGTCNEGDKITRVIEFTNTGKLP
                     LVINQAYGSCGCTVPKYDKEPVMPGKKGKLSVEFDSKNKPGANTKSIMIEANTNPPIT
                     TVAFSVKVKESKSKKSWFK"
     gene            17300..18232
                     /locus_tag="PLL59_01415"
     CDS             17300..18232
                     /locus_tag="PLL59_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HMY22488.1"
                     /translation="MKNYLSLVKFSHTVFALPFAMIGFTLGIYYFFALYKLANPFDAF
                     QPAFLLNTYTKYPLPFFIIYKLVFVLICMVTARNAAMAFNRYIDRKYDEKNPRTAIRE
                     IPAGIITSKNAMIFIVINCILFVVATYCINTLCFYLSPIALLVILGYSLTKRFTFLCH
                     LILGVGLGLAPIGAFLAVTGKFEILPLFFSFAVLFWVAGFDIIYALQDEQFDKENKLY
                     SIPSYFGQDKAKWIARLLHVASILCLLGAGIYGNFVFTYWLGFAVFVALLFYQHFLVL
                     QYGLEKIDLAFFTTNGIASILFSLCVITEIIMHI"
     gene            18271..18681
                     /gene="ruvX"
                     /locus_tag="PLL59_01420"
     CDS             18271..18681
                     /gene="ruvX"
                     /locus_tag="PLL59_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HMY22489.1"
                     /translation="MSKILAIDYGAKRTGFAISDESKIFAFGLSTQDTKNALQYIGQI
                     IPKEKIDTIVIGIPKKLNNEHALLVPQIQSFIKKITQQFPDIQIVEIDERFTSKMAFQ
                     SMIDSGLKKKDRQNKALIDEISATIILQNYLEQQ"
     gene            18748..19311
                     /gene="def"
                     /locus_tag="PLL59_01425"
     CDS             18748..19311
                     /gene="def"
                     /locus_tag="PLL59_01425"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMY22490.1"
                     /translation="MLLPVTLYGDPVLKRKADEIAADYPNLKDMIKNMFQTMYAASGV
                     GIAAPQVGVSIRVFVVDTMQLDEKRKSSPNTGIKKAFINATILEETGEEWKYEEGCLS
                     IPGIREDVYRNAQVKIKYFDEEFNEIIETYDGINARVIQHEYDHIEGILFTDKLKPLK
                     RKLLIPKLNKIARGEVDVDYRVKAYKK"
     gene            complement(19319..19942)
                     /locus_tag="PLL59_01430"
     CDS             complement(19319..19942)
                     /locus_tag="PLL59_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMY22491.1"
                     /translation="MKKLYLFLVLNCLLSVVAFSKDIIQFSGFVRDAESNAVIPFCAV
                     YIQNENRGTITGYDGFFTFAAGKGDTILVKSLGYKSFKIAIPKDLELTSFVKDVSLER
                     ETYELKGVTVRPLPTPEKLRYAVLNLDIPDNLRDLAQQTIENSILNDEISSKTNFDGK
                     ENFNQYVQSQAGYYYNRYGNQRPGISLTDPFAWARFIKDIKNKKKKK"
     gene            20177..21568
                     /locus_tag="PLL59_01435"
     CDS             20177..21568
                     /locus_tag="PLL59_01435"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HMY22492.1"
                     /translation="MQYIKESIALNKERFVEELIDWLKIPSVSADPKFKDEVIKAADY
                     LKTQFEKIGMDNAQLLPTAGYPVVYADKITDINKPTVLVYGHYDVQPADPYELWTSPP
                     FEPVVKTTAVHPQGAIFARGACDDKGQVYMHVKALEMMLSNNDLPCNIKVIIEGEEEV
                     GSAHLGDFLKAYKEKLSADVILISDTGIINNETPAITNGLRGLCYMEVEVVGPNRDLH
                     SGIYGGGVDNPLNVLCKLIGSLKDDNGHITVEGFYGDVIELSEEEKKQINAVPVTESE
                     FEQSIGLPKSFGEQGYTLLEQLSIRPTLDVNGIWGGYIGEGAKTVLPSKANAKISMRL
                     VPNQSSKKIAQLFAEHFHKISPNTVKVTVTEHHGGEPYVTPVDSVEFLAAYKAMETTF
                     GKKPLAMRNGASIPIVALFEDILGLKSVLMGFGLDSDAIHSPNEHYGLFNYFKGIETI
                     PYFYKYYAELKQL"
     gene            21606..21827
                     /locus_tag="PLL59_01440"
     CDS             21606..21827
                     /locus_tag="PLL59_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22493.1"
                     /translation="MISIKEDIITKIQKADDAQLLAEIHRILDANDYIFTAEDVASLR
                     KADEEIDNGNYKTQQQVKSNIQQWINNLK"
     gene            21806..22099
                     /locus_tag="PLL59_01445"
     CDS             21806..22099
                     /locus_tag="PLL59_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMY22494.1"
                     /translation="MDKQFEIIWSPQAELDLHNILLFWLEHNKSITFPEKLYNEILET
                     TESLVQNPYIGKNIQIEDYRRILVRYYLIVYRVEKTEIRILRILDGRQKELKA"
     gene            22189..24141
                     /gene="dnaG"
                     /locus_tag="PLL59_01450"
     CDS             22189..24141
                     /gene="dnaG"
                     /locus_tag="PLL59_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HMY22495.1"
                     /translation="MITRDTIAKILDTAQIEDVVRDFVNLKKTGSILKGNCPFHQEKT
                     PSFVVNPNKNIFKCFGCGVGGDSVSFIMEHEKFSFPEALRYLAQKYHIEIEETHVSNE
                     DKQVQDEKESLLIALNYAAKFYQTQLFETETGEAIGLSYFKERGFLEETIRTFQLGYS
                     QDSFDSFYKQAIADGYNPEVLAKAGLIKEKNDKHYDFFRDRVMFPIHNVSGKVVAFGG
                     RILKSNKTETNTSVYNPKYINTAETDVYHKSQVLYGISHAKADIRKNDVCYLVEGYTD
                     VISLHQAGIKNVVASSGTALTKEQVQLIKRFTQNMVILYDGDAAGIKAAIRGLDIVLE
                     QGLNVKLVLLPDGEDPDSFVSNNGTDFTLDFIARKQQDFILFKASQGIEEAKNDPVRK
                     TEIIKDIISSIAKVDDTIKRQLYIKQCATIVDIPENILTNETNKIRTQLFRKNIEISQ
                     EDATNLIKHVSAEIDHHQQVSKTPHLYFQERDIIRILIEHGDKEMTEEHSVAEYVIFE
                     LVDIEFNNATFGKIIKLFLDAYQNDIPFDKLKDISTIEDKEVKDCLIELMSSPYELSP
                     NWFDKHEIVVKDVNRTFKTDVVSVMLRFRYFKLMEAIQMIDAKIKSSEENGQFEEMLA
                     TMMKKMEYNDEKKKLAKEIQTVIHPF"
     gene            24285..25661
                     /gene="rseP"
                     /locus_tag="PLL59_01455"
     CDS             24285..25661
                     /gene="rseP"
                     /locus_tag="PLL59_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HMY22496.1"
                     /translation="MLLLAISGTATKVILLILSLSILVVLHELGHFIPAKLFGTKVHK
                     FYLFFDFLFPFPNIANFSLFKKKIGDTEYGIGWFPFGGYVQIAGMADETQDAEELKNS
                     TPQPWEFRSKPAWQRLIIMIGGVTVNMVLAMIIFTGLLWKYGEEYLPFKNMTNGIMVV
                     DSLAYEVGLQNGDKIIAIDHQPAGNFEEFQKDLVLNEAKNITIERNGQQQDIQFKDGF
                     LSKLMKQKKATFVLPRVPSIVAQVQDGSGAAKAGLKIGDKITAVNNVPTPFYDQVKNE
                     ITANKGKQIAMTVERNGKPLVLQGTVSPEGLFGFGTQLYSELLKTDRVNYNFAAAVPA
                     GIEKSISTLANYFKSLKMLFTSKEVKVKDSLGGFISMGKMFPDTFDWEHFWILTATIS
                     VILAFMNILPIPMLDGGYVVFLLYEMLRGKPAPEKVQQVAQSIGMFIILGLLLFANGM
                     DFIRAIFN"
     gene            25773..25852
                     /locus_tag="PLL59_01460"
     tRNA            25773..25852
                     /locus_tag="PLL59_01460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25807..25809,aa:Leu,seq:caa)
     gene            26139..26897
                     /locus_tag="PLL59_01465"
     CDS             26139..26897
                     /locus_tag="PLL59_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMY22497.1"
                     /translation="MKKLLLVLLLLGVIVHESDAKIQAGVGIKVGPAMGRWNNGVLNP
                     KLSTAGGTVGFQPGVHAGIQARIWFNKFVGINLAGEFNMQGSTFTRVSGVNSQKVTHK
                     ENQITLPLSVMVGWGNERLRVFGNFGGYFGYNINGKDKEAIYLNGSEQPNSGFTKADY
                     KEKYNSIDVGVRVGGGIQVYVDKKLKSCVTFDINYDYGLMKTFREEEPSYFSNPEKIK
                     ITPSKLLIGVGYSYTFGKSQAEEKPRRAGDMITD"
     gene            complement(26937..27917)
                     /gene="hemB"
                     /locus_tag="PLL59_01470"
     CDS             complement(26937..27917)
                     /gene="hemB"
                     /locus_tag="PLL59_01470"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HMY22498.1"
                     /translation="MLQRPRRNRKSEAIRGLTIETKLHVNHLVQPLFLVDGQKKKNAI
                     KSLTNIHQLSIDNALKEIEACMQVGVNSFILFPAVEDALKNKTATYSYSSKNFYLKAI
                     KEIKKKFPESCLISDVAMDPYSSDGHDGYVENGKIINDKTLPILAKMSLAQAEAGIDI
                     LGPSDMMDGRVAYIREQLDLKGFTDTSIMSYTAKYASAFYGPFRDALDSAPKSGDKKT
                     YQMNPGNKREALIEAELDYTEGADFMMVKPATLYLDIIQDLKQNFPLPIAAYHVSGEY
                     AMLQAAAKNGWLDYDKCLAETLLSIHRAGADVIISYGAKDYATLHKKGKA"
     gene            complement(27930..28859)
                     /locus_tag="PLL59_01475"
     CDS             complement(27930..28859)
                     /locus_tag="PLL59_01475"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMY22499.1"
                     /translation="MEAPFHFPHQKSFYKGKVRDVYNIDDQYLVMIVSNRISAFDVVL
                     PKPIPYKGQVLNQIAANFLAQTSDIVPNWVIDTPAPNVTIGKVCEPFRVEMVIRGYLA
                     GSAWRAYKNGERTLCGVALPEGMIENQKFDTPIITPTTKAVVGHDEEISKEEIIARGI
                     VSKSDYEQLEHYTQQVFQRGTEIAAKQGLLLVDTKYEFGKIGDTIYLIDEIHTPDSSR
                     YFYADGFETRLKNNEPQKQLSKEFVREWLIDNNFMGKEGQTVPEMTDEIVQNISNRYI
                     ELYELILGKKFEKADTDDTEQKIRLSLEKLGLK"
     gene            29040..30179
                     /gene="dnaX"
                     /locus_tag="PLL59_01480"
     CDS             29040..30179
                     /gene="dnaX"
                     /locus_tag="PLL59_01480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HMY22500.1"
                     /translation="MENFIVSARKYRPAKFLDVVGQEQVSETLKKALSSGKLAQSFLF
                     CGPRGVGKTTCARILAKAINCENPGADFEPCNQCTSCVSFSQNASFNIFELDAASNNS
                     VDDIRTLVDQVRFAPQSGKYKVYIIDEVHMLSPGAFNAFLKTLEEPPSYAIFILATTE
                     KHKIIPTILSRCQIFDFKRISDKDIVRQLQKICDSEGVQAEPSALHIIAQKADGGLRD
                     ALSLFDKIVSYAGKQVSYQDVISNLNILDYEYYFKITNFFMLEDAASVLNAFNEIINN
                     GFDGEMFLLGMAEHFRNLLISKDEKTVPLLEAAEDVKAKYLAQAKLLNETFLLNALSI
                     INQCDIEYKTTKNKRLHVELSLLKICYLNTQIESTISLILGEKKN"
     gene            30227..30868
                     /locus_tag="PLL59_01485"
     CDS             30227..30868
                     /locus_tag="PLL59_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22501.1"
                     /translation="MQLEDESVNSLHTKTIQAVIADTNVLKQHVDNTTTSQENKQQAT
                     LERTELNTNAIASVTAQKSKRELPKTSSIGSMFKNLEKQNVEKKVEAKLELTPENTIV
                     IWNKFLNENKANLHSSFLSAAQSHPPVLEDDKIVFTASNNVTLEMLQLHKMDITAFFR
                     LRTTSASVVPVFVLNRAESPIKDYKTNKDRLKDMIDSNASIMKLIEKFDLNME"
     gene            30884..31246
                     /locus_tag="PLL59_01490"
     CDS             30884..31246
                     /locus_tag="PLL59_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HMY22502.1"
                     /translation="MNKKTVVIGASAKQDRYSNKAVRMLQYHHHDVIALGFENAQIND
                     ISIQTIWQHYDNVHTISLYLNPQRQKEYYSYILSLQPKRIIFNPGTENDELQQLATQQ
                     NIDTLEACTLVMLTTEQY"
     gene            31317..31769
                     /locus_tag="PLL59_01495"
     CDS             31317..31769
                     /locus_tag="PLL59_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22503.1"
                     /translation="MKKQILMLFAVVFSFTAMSFAQGNPNAKMKPVKLQKVSVEQKAI
                     NATDTMQKVAQLSKEQYQQILAINQDFFKQQHTIKSEMRKDTSANDIAFKEQLIQLSK
                     SRKASINKLLTETQKTVWKDYKKINMPNKKNKSSKQDMFIDDESLDEM"
     gene            31853..33268
                     /locus_tag="PLL59_01500"
     CDS             31853..33268
                     /locus_tag="PLL59_01500"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HMY22504.1"
                     /translation="MLTEQDKIKHLYNRAGFGYALSDIQSGRSLSTTIEKLFSNKTYR
                     SLQTVTFTEVESAKEKMKSSPKDAAKEIKKLLKDNISDLNNLWFQEMIVSDAQLQEKM
                     ALFWHSHFACRSVNPYFDQQYLDIIRKHALGDFSTLLFEISKTPAMLQFLNNQQNKKD
                     HPNENFAREVMELFTLGRGNYTETDIKEAARAFTGYGFDKVGSFKFKKQLHDFGVKTF
                     QGKTGNFNGDDILKIILGNKNCSTFLVRKIYSFFVNEQVDEQIVQSLSAQFFQSNYNI
                     KQLMKTIFSSSWFYDEKNIGNKIKSPIVLLTGMFRLVPTTFTKPENVLFLQRSLGQVL
                     LFPPNVAGWKEGKTWIDSSSLLFRMQLPQIIYFDKSLDMEPKEEIPEMYAAMQKIKSD
                     YMKSFIAKKISVASDWTAIISHFMLSKSVTNDAAYFVLQQPADAMTLKAVVANSDFSS
                     KQNEIKSTIINMMSLPEYQLC"
     gene            33293..34483
                     /locus_tag="PLL59_01505"
     CDS             33293..34483
                     /locus_tag="PLL59_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HMY22505.1"
                     /translation="MKRRTFLQTSGLATMSMLVPKFLKARENINSISSNKILVVVQLS
                     GGNDGLNTVVPYENDLYYQARPSIAIKKSEVLKLDTLLGLHPNLHGFKKLYDAGNMCL
                     INNVGYPDPDRSHFRSTDIWQTASNSNEYLSTGWLGRYLDVVCNPCERPTQAIEIDDT
                     LSLALKGTQAKGLAFKDPKKWYNTTTDNFFKLLSKQHSEAHEHDTAGYLYKTLSETIS
                     SASYIYNTSKIYQSSSVFPNHAFGKNLKTIAELIVSGVQTQVYYVTLTGFDTHNNQQK
                     RQGVLLQQLADTIDVFFNDLKKSGRADDVLLMTFSEFGRRVQENASNGTDHGTANQIF
                     LFGNKLKKQGIYNEAPNLSSLDDGDLKYTVDFRNVYATILHNWLQTNEHLILSNSPSL
                     LNFI"
     gene            34610..35293
                     /locus_tag="PLL59_01510"
     CDS             34610..35293
                     /locus_tag="PLL59_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22506.1"
                     /translation="MISLHFSVSAQDVIEIVDPYEMKASRSKEADTFYFTQNTNAILK
                     KKLDTLRTYDVFLEERLTPFGKAYMCNGNEVTKQKYLEFKRFWGASAACTPCLLYTYN
                     DKNELKYVAYQYEECLCGSYKEYYTDGTLKVEGQFKQNTTGSWENLKSKNVCNVRDGI
                     WIYYTETAVEEKRETYIDGKLKGTTFSPSQTSPVKQQAAKQIEDSTQTNEPKKNWFKK
                     LKDKNTDEQ"
     gene            35465..38674
                     /locus_tag="PLL59_01515"
     CDS             35465..38674
                     /locus_tag="PLL59_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMY22507.1"
                     /translation="MKSVNKIILFFVLCISSFTLLAQTKIKGKVTDGKEPIIGANITI
                     KGTTEGTVSDLDGKFELSTNQATPLTLVISMVGMATSEVKFDGSQEEISVSMSEETSL
                     LNDVVVAASRVEEKILESPVTIEKMDPKAIKTAAAADYYDDLAKLKGVQTINGSMTLT
                     SVNTRGFGGISNTRFVQLMDGMDNAAPLLNFPTGNIVGIGELDINNVELVPGAASALY
                     GPNAFNGILLMNSKNPFEQPGFSMQVKGGFAQANNGYGVKPLGSAALRIAHAFKSKKT
                     GQDFAAIKANFSIFQGTDWYANDYTTDRSLGTQDNVGNPNFDGLNLYGDEARIFVPYG
                     AVRGNLIGSASNSLQNSPLFQAGVTAAVTAQVNAQLPAITTVVTATVRQQAYDNAIAG
                     GADVATANTMADAYIASPAGQATIQAHVQAQVQALIDSNVATQLPIQANARATSKVDS
                     ISSLLSLDLRRSGLSEDRLLDNHKAQSIKGDFGAYFRPFKDKSYEFSYNYRIGFGNSV
                     YQGSERYALRNFLQQFHKAEIKSKDFFIRSYMSQTKDGDSYNLSALGAYSNEAVSPSG
                     SQWVPTYLSSYAGPLIGAALQTSAANVLANAAALTSMAHDTARARADRPWNNLTAAEQ
                     QAVIESVRTGLFQRGGAGFIDNSRLFHTEAMLDLTRWTGKWIDIQIGGNHRLYSLSTQ
                     GTVFNEDPNGTGQYKRIKINEYGAFLQLQRKFYKDMFKIQASVRFDKNQNFKLIISPR
                     AAFVATLGKDRNHNIRVSYQTGFRNPDSQAQYIYFPTTNILLGGTKQNAERYGIYNGG
                     AYTMDSYNEFVATALAGAPDVTKLVVSDFDYVKPEQLQVVELGYKSVLFKKLMIDVNG
                     YFNIYKDFITQITVVSIDSTYHKGVLLPGVAQVISGSARTATTWRPYVNIPGKTFSYG
                     AGIGIGYALPKNIMARVSYNYMDYKIKGGKPSVGTANDLGFNSSKHQVFVGFSGDRVW
                     KGLGFAIDYRWQSAIDWSSDFADGTVKARGMLDASLSYFVEKAMTTFKIGATNIAGPT
                     YRTNVGGPFIGRTFFAAITFDQNKLWKEHHNEVTGAKEF"
     gene            38890..40065
                     /locus_tag="PLL59_01520"
     CDS             38890..40065
                     /locus_tag="PLL59_01520"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMY22508.1"
                     /translation="MQEVFIVSMARTPIGALSSTLSGLNVIELGKTVVKAAMERAGVN
                     GDQIDEVYLGNVLQANVGQAPAQQVSIAAGINTKTPCTTINKVCASGMKAIMLGAQSI
                     MLGDNHIVVVGGMESMSNAPHYLPSGRTGIRYGNGEILDAISRDGLQDPYKKYMMGNA
                     GEVCAKHYSFTREDQDAYAIASYKRAQDAYANGYFKDEIIPVVIASRKGDITISEDEE
                     YKKFDEAKVSALKPAFEKEGTITAINASKINDGASAMVLMSGDKVKELGLTPLAKIIS
                     FADASQEPEWFTTTPSKAIPKALQKANLTTEQVDFYEINEAFSVVALANMKEMNIAHD
                     KINVFGGAVALGHPIGSSGCRIVATLISVLKNKNGKIGAAGICNGGGGASAIVIEKV"
     gene            40242..40916
                     /locus_tag="PLL59_01525"
     CDS             40242..40916
                     /locus_tag="PLL59_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22509.1"
                     /translation="MKNLMKLFTVLVVVMFSTSTFAEELGLKVANMNLYAPTAKGKSS
                     SGGGKAFEGKGSKQISIGVGLSSYIGAFGKGGFYSYTGSRGGYYWGRGAFWYSGTLSV
                     QAEFGVHDYVGVGLVTGIGGRAVGGGGVLYVPVGILANFHFFQLVADKKGLGIGDALD
                     LYVGLNFGTGLGASLPKGSGNNVSGIIFAGPQFGVKYFFNPKIGVYGEFGYGKSVFEA
                     GVSIKL"
     gene            41004..41660
                     /locus_tag="PLL59_01530"
     CDS             41004..41660
                     /locus_tag="PLL59_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22510.1"
                     /translation="MRLPLYFICCVVLLFSAVAVYAEENISSIDKEKKEKKQKNKADD
                     GVEGAAAKGDFVIQPAINFGFHSGFVKQYSGFGYGYAYTGLIPGVTLNLDYAAHDYAS
                     VGLYYGVSFQKFTVSKVSLLHHTFGARVQFHWWQMLADRSGKELLADKIDFDIHAHIG
                     GYYLTEKNLISDQKLKHLGVNAGGGIGFKYYFVKHFGVALDAGYEEASWLKLGLAIKI
                     "
     gene            complement(41657..>42481)
                     /locus_tag="PLL59_01535"
     CDS             complement(41657..>42481)
                     /locus_tag="PLL59_01535"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HMY22511.1"
                     /translation="IPQANATGNLTIETLHKVDSVRQLSNKNYELTVHKINEQGETVA
                     VKIYTCKYLFINAGVVGTMNILLKSKHLGGLANLSDEVGKHWGNNGNIMAMRSNLRED
                     TGAEQGSVPVSAYGDLNNPITPLLAEQAPFPIGLELRQLLALAITKNPSRGHFKYNPA
                     TDNADLYFDKSQMEISRQAMKSFVDRLNAANGGTLEQFWYFNGKGYGDDFTYHPLGGA
                     VYGLASDTTGRLKGYSNLYCLDGAAMPGFSCCANPALTIAALAERNMEKILAEDFS"
BASE COUNT        14415 a         7048 c         7578 g        13440 t
ORIGIN      
        1 taattaagta attaagtaat gtaataatga attttgaaaa gtatacattg ttacattgtt
       61 ttattatttc attctactat accaattcca caattgttgc aattcccatt ccgccaccaa
      121 cgcaaagcgt tgctaagcca gttgatttat tagtacgttc taattcatct actaatgttc
      181 ctaatatcat tgcacctgtt gcgccaagcg gatgtcctaa tgcaattgca ccgccattta
      241 cattcagttt ttcatctgga acattaatgt ctctttgcaa gcgtaacacg gcagaagcaa
      301 aggcttcatt catttctact aaatcaatat cattaatagt catacccgct ttttgtaagg
      361 caatttttga agctggtcca gggcctgtca gcatgatagt tggctcggtg gaagtaacag
      421 cgactgaacg aataatagcg cgtggcttca agcctaattt ttcaccaatt tctttagagc
      481 caattaatac caagctggca ccatctacaa tgccagaaga attgcctggc gtatgtatat
      541 gatttatttt ttcgagcgtt gtatattttg ataatgcggt attatcaaat cccatttcgc
      601 ccatcatttg aaaagacggc gttaatgatg aaagaccttc taaagtagta tcaggtcggt
      661 tatattcatc ttcttctaaa attgccaagc cattgatgtc aacaattgga acgatagatt
      721 ttttaaatcg tccttctgct ttggcaatgc cagccagttt ttgagagcgc aatgcataat
      781 tatcgcaatc tgttctagaa aaaccttcta tagttgcaat taaatcggca ctgatacctt
      841 gtggtacaaa atcggtatga ttgttaactc tcatatctaa aacccaagcg ccgccatcat
      901 atcccattgg cactcttgac atagattcaa caccgccagc aatgattaaa tcttgccagc
      961 ctgaacgcac cttcattgct gctaagttga ctgcctctaa accagaagcg cagaaacgat
     1021 ttaattgaaa tccaggaact gtagtggcat aatctgccat cattactgct gttttagcga
     1081 tgttgccacc ttggtcacca attggtgtta cacatccaac aataacatct tctactaatg
     1141 tagtatctaa attattacgc tgctgtaatg cctttagcat ttgtgctaat aattctgtag
     1201 gtgtgacttc atgtaatgca ccgttttttt tgccttttcc gcgtggcgta cgcacgcaat
     1261 cgtaaatgta agcttctgcc attgtattta ttttaagttt ataaatttat aattttgtca
     1321 tcatcagcct ttaaaaagtc gagatgtaaa ttatgtcaaa tatttattag gaaaatcaac
     1381 atttacgcca attaaccaaa cgttcgtttg ataaatgtaa aaaactaatt ttaaaaaaca
     1441 atgcttacat tgattttctt atcaaaagtt cagctacaaa atactatgta ttcgtaatcc
     1501 attctgctac gaattcaaac ttaattttac atgatgatag tcttgcttat tatctatttg
     1561 tgatgaaata atggaatgta gctttttgtg tgataaaata ttattttagg tgacgcaaga
     1621 tatttcactt aatgaattaa caataaaccc atgaaaaaaa tattgctact tggttctgga
     1681 gaattaggaa aagaattgac aattgcatta aaaagattag gacaatatgt aatcgctgtc
     1741 gattcatata atgatgcgcc agcacaacag ctcgctgatg aaagagaagt cataaatatg
     1801 ttagatggtg aagcattaga ccagattgtt gcaaaacatc aacctgattt aattgttcca
     1861 gaaattgagg cgattcgtac agagcgattt tacgattatg aaaaacaagg ttttacggtg
     1921 gtgccaagcg caaaggcagc caattttact atgaaccgta aggcaattcg tgatttagca
     1981 gctaaagaat taggcataaa aactgcaaag tatgaatatg caactagctt agaggagttg
     2041 atagctgcaa caattagaat cggaatgccg tgtgttgtaa aacctttaat gagttcttct
     2101 ggtaaaggac aatctgttat aaaaacaaaa gaagatatcg aaaaggcatg gagctatgca
     2161 caagcaggaa aacgcggcga tcagatagaa gtaatcatag aatcatttgt tgatttttat
     2221 gcagaaataa ccttattgac agtcacccaa aaaaatggaa atactttatt ttgtccacca
     2281 atcggacata gacaagaacg tggtgactat caagaaagtt ggcagcctgc atatgtgaag
     2341 attgaagact tattagaagc agaacgaatg gcagcagtgg ttacacaagc attaagtggt
     2401 gcaggtattt ggggtgtcga attttttctt accaataatg gtgtttattt ttcagagcta
     2461 tcgccaagac cacacgacac aggcatggta accttggcgg gcacacaaaa tttctctgaa
     2521 tttgaattgc atgcacgtgc catattaggc tatcctattc cagaaattac tttggaaaga
     2581 gtaggtgcta gtgctgttat tttgtctgag agtgatggga ataatccaag atattttggt
     2641 gtagaagatg ccattaaaca aacaaaatca gatgtacgta tttttggaaa accaataatg
     2701 cgcccttatc gtagaatggc tgttgcatta gcgtatgatg tgcttggaag cgatgttgag
     2761 atagtgaaaa atcgtgcgat tgaaattgca aacaagataa gtgtgaaaaa tatttaataa
     2821 gtccgagaga tgctaaattt gtattgtgcg attaaaaaat ctaaaaatac atgatataca
     2881 atgcttattt taaatcgccc ataggcatac tcgaaatcaa atcgtcagcg acacatatac
     2941 tttcgataga attcgtagaa gaatcagatt ttgaaaagca acaaattaat gatttaattc
     3001 atgattgcat actacaacta acagcttatt tttcccacct acttcatcag tttgaattgc
     3061 cattagcatt taatggttct gcttttcaaa ataaagtatg gcaagaactt caacaaattc
     3121 cgtttagtac tacatgctcg tatttacaat ttgccaaaaa attaggtgat gccaaatgta
     3181 ttcgagctgc cgccgctgca aacgctagaa atccatttgc gattgttgtg ccttgtcatc
     3241 gtgttattgg tagtaatggc aaactagttg gttatgctgg tggcttgtgg cgaaaacaat
     3301 ggcttttaga acacgagcag gcaatatcat tacaaacaaa acttttttga ttttatctac
     3361 agcagtatta ttaaaattta actcaggaac ttttcttgct ttttgtacat ttgtactatt
     3421 ttaattattc aatcatcaca cataaaccaa tgtaaaaatg ggtaaaagag ttttaattat
     3481 tggagccgga ggcgtaggaa atgtagttgt tagaaaatgc gcatcagttc cagaagtttt
     3541 tagtgaaatt atgttggcaa gccgcaccaa gtctaagtgc gatgcaattg cagctgacat
     3601 caagcacatg aacataaaaa cagcagcatt agatgctgat aatgtaccag aaatcgtggc
     3661 acttatcaaa gagtttaagc cggatttagt gatgaacata gccttgcctt accaagattt
     3721 aaccattatg gatgcttgtt tagaaacggg cgtacattat ttagatacgg caaattatga
     3781 gcccaaagat gaagcaaaat ttgagtatag ctggcaatgg gcttaccaag agcgctttaa
     3841 agaaaaagga ataatggctt tgttaggttg tggtttcgat cctggtcagt ctcaaatata
     3901 tgtagcgcac gcagcaaagc atcatttcga cgaaattcat tacttagata ttgtggactg
     3961 taatgccggc gaccacggac aagcttttgc gactaatttc aacccagaaa ttaatatcag
     4021 agaaataacg caagacggta agtattggga agatggtgaa tggaaaatta ttccagccat
     4081 gtctattcat aaagcaattg attatccaaa tatcggtcct aaagaatcat atttgttata
     4141 tcacgaagaa ttagaatcat tggtaaaaaa tttcccaaca ataaaacgtg cgcgtttctg
     4201 gatgacattt ggtgctcaat atctaaaaca tttagaagtg atgcaaaata ttggtatcac
     4261 ttctattaaa ccaattatgt tccaaggtag agaaattatt ccaatcgagt ttttaaaagc
     4321 attattgcca gagccaggtt cattaggtga aaattattca ggtgaaacct caattggttg
     4381 ccaaattaaa ggtataaaag atggtaagga taaaacgtat ttcatctgga ataactgtaa
     4441 acatcaagat gcttataatg atgcaaaagc acaaggcgtg agctacacta cgggcgtgcc
     4501 tgccatgcta ggtgctaagt taatgttaga aggcatttgg atgaaaccag gtgtgtacaa
     4561 cacagaagaa atggatccag atccattcat gtcgcaaatt ggtggctatg gtttgccttg
     4621 gcacgaagaa ataaatccag ttttaccgca tgagtattag ttcaatttaa ttaactagaa
     4681 tattgaaact tcacagtttt ctgtgaagtt tttttatttt ataaaattga atttatatca
     4741 ttggtcaaaa aaatcgtaat ttgtataagc atgcaaataa aatctaacta tatagatatt
     4801 tttaatcagc agatttttgc tgctgaaatt tctattgaaa atggaaaaat aaaatcgatt
     4861 gtaaaaataa atgaagctgt agagacgtat attttacctg gctttgttga tgcgcacgta
     4921 catattgaaa gttcgatgtt ggtaccaaca gaatttgcaa aaatagcagt catacacgga
     4981 actgttgcca ccgtttctga tccacatgaa attgcaaatg tactcggtaa agcaggtgtt
     5041 tattatatgt tggaaaatgc caaaaaatca ccattaaaat tttatttcgg tgcaccatca
     5101 tgcgtgcctg caacgacatt tgaaactgct ggagcagtag taaatgctga tgatatagat
     5161 gaattaatgt ctttagatga tattaaatat ttagcagaaa tgatgaatta tcctggtgta
     5221 ctacatcgcg ataaattggt gatggataaa attacaattg ctcaaaaaca caataaacca
     5281 attgatggcc atgcgcccgg attgacaggt cacgaggcat tgcaatatat tagcgctggt
     5341 attactacgg atcatgaatg ctttacattt gaggaagcaa aattcaaatt agaacatggc
     5401 atgaaagtgc tgatacggga aggaagtgcc gccaaaaatt ttaatgcatt aattgattta
     5461 ttgcacgaat atcctgatga tatgatgttt tgtagtgatg ataaacatcc tgatgattta
     5521 ttatttggcc atatcaatga tttggtgaaa cgtgcagtaa acaaaggagt ttcattgttt
     5581 aatgtattga aagcagcatg tgtaaatcct gttttacatt atcaattaaa tgtaggtttg
     5641 ctacgagttg gtgatgaagc tgattttata cgcattaata atttagaaga tttttccata
     5701 gtagaaacat acattggcgg tcattgcgta gcaaaaaata aacaagattt tatcgaaaca
     5761 aaaccagaaa aaatagtcaa taattttagt gtaaaaacaa agcaaaaaga agattttaat
     5821 ttgcttgtaa aacaagaaaa agttcgtgta attgaagcaa ttgatggtca acttataaca
     5881 caagaatata taggcaaaat agtagtagat aatgactgtt atgcacatgc aaatattgat
     5941 gacgatatct taaaaatggt agtagttaat cgttaccatg acgcaccaat tgctgtagca
     6001 tttgtcaaaa atttcggttt gaaaaatggt gctatagcct cgtctgttgc acatgattct
     6061 cacaatatca ttgctgttgg tgtaaatgat gattgcattt gtagagctgt aaacactatt
     6121 attgcagcgc aaggtggtct tgcagttgtc gatgaggaga attgtgatat tctgcctttg
     6181 ccggttgccg gattaatgag taatatgagc tgcatagcag tagcagaaaa atatacgaca
     6241 ttggtcaata aatcaaaatc gcttggctgt acattacatt cgccatttat gacattatct
     6301 tttatggcat tgttagtaat tcctaattta aaactaagtg acaaaggttt atttgatgcc
     6361 atcagattct aattttgcga tatagaaata aaagattagt acatttgtaa ttgaatatta
     6421 taatcatgac ttacgaccat ataccatcgc cgtcttttgt gttggaagaa aataaattaa
     6481 taaaaaatct tcagctccta aaaagcattc aagatgaagc agatgtgcat ataatttgtg
     6541 cattgaaagg attttcattt catgctgttt ttccaacagt aaaaaaatat attcatggag
     6601 caacatctag ttcactacat gaagcaaaat tggtatatga aaaaatgaac gttagagcac
     6661 atacttattg tcctgtttat ataccaaaag aatttgatga aatccaacaa atatcatcac
     6721 atattacttt taattcctta tcgcaataca atacatacaa acataagatg atacctgaca
     6781 tctcttatgg attgcgagta aatccaggct attcagagat tacgacggaa ttgtataatc
     6841 ctgcttcgcc taactcacgt ttgggcatcg ccagaaattt aataggagat gttttgccag
     6901 caggaatcga aggattgcat tttcatgcgc tatgtgaaaa tacatcttac actttagagc
     6961 gcgtgctgga gtcatttatc aaattgtatc atccattaat tcagcaagcg aaatgggtaa
     7021 actttggtgg aggccattta attactagaa aagattatga gccacaacat ttgatacaaa
     7081 ttttaaaagt ttttaaatcg aaatatccta atctacagga gataattctt gagcctggaa
     7141 gtgctattgg ctggcaaact ggtgtgttgg tgtccactgt tttagatatt gttccaaatg
     7201 gaaatattcc aacagcaatg ctagatgttt cttttacttg tcacatgcct gattgcttgg
     7261 aaatgcctta taaacctaat gttttgagtg ccgtagaaaa tggtacattc gaatataact
     7321 taggtggaat gagctgtttg gctggcgatt tcattggcac ttggaaattt gaaaatgagc
     7381 ttaaaattgg agataggatt gtgtttgacg atatgataca ttatacgatg gtaaaaacat
     7441 cgacatttaa tggtattaat ttaccgtcaa ttggtgtgat gaatgagaac ggcaaatttg
     7501 agctaattcg ctcttttagt tatgctgatt ataaaaatag attgagttaa agtattaaat
     7561 ttatactatg caaaagatat tagataatct atacagacaa gcagagcgaa ttatttcgtc
     7621 agatacctta gtgtcgaaat tgattgatac tgtattttta aagcttggcg aagcatcaga
     7681 atctttttat aaaatacaag ataacattat agcgcttaca cgtatgttgc gtgcttgggt
     7741 aaaaggcgat tataaaaata tttctacagc atcaatcatt gctgtggttg ccgccttatt
     7801 atattttata aatccgctgg atatcattcc agattttatt ccaatcatcg gacaattaga
     7861 cgatatcttt gtgttgagtt atctcatcaa aacactcaat aaagagattg aaagatttat
     7921 ggcttgggaa gaagaacaag catcacacta atcctttctt gtacgcgatt ttaagcaagg
     7981 cagcagtatt gtttacgttt aatttagcca gcatattttt acgatgtgtt tccaccgtat
     8041 ttttactgat aaaaagtttt gttgcaatat cttgagaagt aagacctaat gaaattaatt
     8101 gcaaaatctc taactcacgt tgtgtgattt gtgccgctaa ctcatcatag tttctgtcat
     8161 caacttcatc gctattggaa atagaattga ttagcttttc ttgtgcttct tggtctaaaa
     8221 atgatttgtc ttggtacacg ctgtcaattg cttttagcaa atcatcttta gaaatatttt
     8281 taagcacata acctttagca ccaatttcta aacactcttt tataaaacga ggttcgctgt
     8341 gcatggtcag catcaaaatt ttaatgttag gattgatttt ctttaattgt tttgtggcct
     8401 ctatgccgtc catttcaggc atgtttatat ccatcaatac aacatctact ttttgagttt
     8461 ttattctgtc aatagcttgt ttgccattat gtgctgtgcc tacaattttt atatttggtt
     8521 catcatctaa taaagaaact attccgtcta aaaaaatttg gtggtcatct accacaaata
     8581 cattgatttc ttgcatacgc tgttgtctaa ttattttaca aagttatttc taattcatca
     8641 attaaaagcc gatttaccaa tatttaataa atgaaaacca tttcctagtt tctaagtagt
     8701 tataattggt ttcattttca tacgcttcgc gctcaaatga aatatttcga taagcttgtg
     8761 ttacattttt gtattttaaa agtaatataa aaaactcaat ataatatagt attacaaata
     8821 gtattacaaa taattccatt tgctggtaga tatgtattaa ctcgtgattg ataaatgtct
     8881 tgtcatattt taaatctttt ttttctacaa ttataaaagg aaataaggta ataccagcta
     8941 ttttttttgg cgtaaaatat ttaataatat taggactgat gataatccac cacatgtatt
     9001 tcttttaaga aagatacata aaataagcaa gcaattacac aggtacttta ttttcgagtc
     9061 gccattgtaa atctgccaaa actgcttgtt tccattgctc acttccaatt gttgtgttaa
     9121 acattttttt atagcttgga atatctaatg attctaaatg taattttaca gtcttgaata
     9181 attttggcaa tccttctcct ggcacttgta catggtgcaa tccaccactg tatgcaatca
     9241 ccatttcacc tttatcgagt ccatttaata tatcgactac accagatttt acagtcattt
     9301 tatttccgta caaatctaat ccattcttgc gtttcatgcg tccttctggt gcaattaaaa
     9361 tgatagaatc ttcataaata gattctaaaa aattatccca actgtcgtct ctttttcttg
     9421 taatcgaaat cataccagga ctaaataatt taaaaaatat gcctactaat ggcctgttta
     9481 atgatttgtc ggcaccaggt gctaccatgc gttttgacaa ttttctaaga aaatgatttg
     9541 gcaaaaatcc aagaaatata aactcgtata atgtcgtatg attgagcaat aaaatcaact
     9601 ttgtattgtc ccaattgatt ggcgttgaag gccaagtaat ttgtatggta taaaataatt
     9661 tagaccaata ttttaaagtt agaagaaaga aataactaat taaactttgc atcttaaaag
     9721 ctggaattca atatgcaagc tatgaaaaat tatactgtta aaatagtata gtgcaacatt
     9781 tatttttcgt gatatattga tgtagttttg aaagattgat ttacaagata tcttattatt
     9841 gttggtaaaa ataaaatatc attatcatga aaacgatttt atgtcgcatc atttaattcc
     9901 atttttctaa tagaaggtga aataaaataa gttacgatta ccaccaagca agtcatacaa
     9961 ccaccaaaaa ttactgcggg cactgtgccc attgcggcag ctgtggcacc gctttcaaac
    10021 tctccaattt cattggaaga gccaataaac atagagttta cagccgatac acgtccacgc
    10081 atctcatctg gtgtggagag ctgaacaata gtatggcgaa tggaaacgct gatgccatct
    10141 aatgcaccac tcatcatcag acaaaacatc gaaaaccaaa atattttgct aatgccaaat
    10201 aggataatga acacaccaaa tccgaatact gctaataaga gaataatgcc agcatttttc
    10261 tttataggaa aataagcaag atacatcagc attataattg cgccgatggc ttgcgctgct
    10321 cttaaaaatc cgagcccttc agcacccaca tgtaagatgt cttttgcata cacaggcaac
    10381 aaggcaatgg cacctccaaa tagcactgca aataaatcca aagtgatgca tgataggata
    10441 accttattat gaaatacaaa ctttaatcct tgcagcgcgc tttctactac agattcgttt
    10501 tgcggtacaa actgaacagg ttttttttct atatataata tagccaatac tgcaatgctt
    10561 agaatgatta caataataga aagcgtatgt gtaattccta aaatacctaa tagcaagcca
    10621 ccaataccgg gtcctaatac tgcacctaat tgccatgcag aactactcca agtaatggca
    10681 ctttgataaa catctcttgg cacaatttga gcagctaaac caaatgatgc aggacctgaa
    10741 aaacttcttg caatgcctgt tagaaagatg atgacgaaaa taatgatcac gacattgtct
    10801 ttgccaatgc tagcaatcat tgaagtgtgt gtgaaaaata gcaaagtagc acataaaatg
    10861 gtatatatac caacaaacat gactaacatg gttcttttgt cagatttgtc agcaatgtag
    10921 ccgccataca aagcatttcc aatggctggt atagcttcgg caagacctat aaatccgagc
    10981 agtaatttag aatttgtgag ttcataaatt tcataaccaa taataattgc ttgcatagtg
    11041 acagcaatcg ttatcaaaaa tctggctaat acgtatgctc tgaattcttt gtaccgtaat
    11101 gctatatatg gatctttctt tggcgttgac atgaattgaa tctaattttt tctgctaata
    11161 ttatagctta ttcatgtatc cttttaaatg tggtttttct tgtgttttat caacgatatc
    11221 aaaatcgaat ccaagatttg ttttttcatg acggaatata acattggcag gcaagaaaat
    11281 aggcagttta aacaatacgg tgagttcata attttctgga actctacttt caaattcaga
    11341 aacaattttg ccaagattcc acattccgtg aatgatatgt cctggaaatc caaataattt
    11401 tgcggacaga gaatgtaaat gaattggatt gaaatcgcca gatgctttag catattcaaa
    11461 tcctaaattt gttggaagac tccaagttga ttttatactg tttgcgctaa gttctggttg
    11521 atgccattct aaaacgctgc cgataccttc ttttcctttg tataaataga tgcttgtgtc
    11581 ttcccaaatg cgtgtgccat ttacatctac aatactgatg atttcaaagg cttgcccttt
    11641 ttcgtgtgca attaaattac ctaatttgca agttgcagaa aattcttcgc caacatataa
    11701 tggtctgtat tgcttgatgg aatttgcaaa atgtactaat ccagctaaag gaaatggaac
    11761 ttcaggttgg ctgagtaatt tcatttgcaa tggaaaaaca aatgtatgta agtaggtcga
    11821 agatactgtt ctttgattgt caaaaccaca gactttatta tatttcgaca tttctgaagc
    11881 ttgtataact gctttatcca aatgcacatg gatatctggc aaatcgcctt ttcctttttt
    11941 tataaatgta gtggcgatgc ctgcatataa tgttgctgct gacggaatcg acgtaaggtt
    12001 aattattttt gacatgttaa tattttattg cgaaatatac acaacaaaaa tagcaaacat
    12061 catagtaaaa agataaaatt tgtgatgtaa taaaacttta atctaaactt aaatggaaat
    12121 cagcataatc aatatataaa aagcaatgaa aatgcttatt tcatttttaa tgtatagcgt
    12181 ccgcttttta ctgctttgcg caatcgtttt ctgctgccgt catcaacaat catagcgcca
    12241 gccattgtag ataatccacc taaaagtgca cctaaaaatg tagtaatcat gatgagcaca
    12301 gcgccagtaa agctggtttg tagtgaatct gagaataatt gcgccacttt tgcactcagc
    12361 acgtgttggt tttttatgtc taaaaataaa gcataaatca gccaaaataa tccgacacct
    12421 ataaagccag caatgaaatt atttccaggt ctgttagaca aaataaaacc tattataaaa
    12481 caaataacaa aaggtatgta ccacggcaga aacatcgtag atactgcact taaaatacta
    12541 atcaataaaa cttttacaaa aaacatagct tggattttat tgtttattta taatttttaa
    12601 agcttcttct ttgttggcaa aaaagtgcaa gttgttgtat tcacctttac tccatttgtc
    12661 aatataattt gttgatttga tactgccaac attaccactt tgaccacctg gataaatgcc
    12721 atagcatgtt ggttttcctt gtgcaaaatc aacaatcatg cgccatgaag gtcctgcgcc
    12781 ttctgatgtt gcattgacaa tattgttata accgccaatc ggaatatcgc ttactgaaaa
    12841 tgctggaata cgtgctaagt gtgtaacagt cgtattttta taatgactcc attgcaaatt
    12901 tttatctttg ttttttgcat aatcttcaaa aaatgctaca gttttttgaa aggcaagttg
    12961 tacgacatct tgcgcttttt caatgtttgc cgtgtttttt atatcaaata aaacaaaatc
    13021 tggttcgtgc aaaatcaaat tagcggtagt ataattttct ggcaatctaa atgagctttt
    13081 tgtttttaat aattcatcat atgttgtttc ttcgatataa ctccagagtt tatagaaata
    13141 actaggtgca gttagattta tatttgcttc gtaattccaa gattttaagg cacgtaaaat
    13201 ttgattcgaa gtctcactat tgttgctgac aatagtagcc aatagcgtag gaagtgtttc
    13261 tttggccaat agacttaaat tattgctttg caatgctgcc atatctttcc atgatgattg
    13321 taccgttgaa gtcaatacgg aattgattac tctgtttcta taattttcgt agtgaccact
    13381 tgttgaataa ggataattat tgtccacagg attttgattg gcactactac aaaaattacg
    13441 ctcaggattt aagatatgag gattttgtgc attcggaata cgcgctttta gttttgtaat
    13501 atcaacattt gctaatggtg ttacaaaaat gccatctgta aattgcgtgc gtagcataaa
    13561 atttccttgt tgttttatag caatattatt ttgcgcatca gcataaataa agttctgacc
    13621 tggacaagaa aaataatcta atgctgcaag ataatcttga tgattttttg ctttatttaa
    13681 ataataaaat gtcattaatt cgttcgatgg ttcttcggca cgccaatatg tggcaatgtg
    13741 ttttttgtcg tttgtttcgc cataattttc atcatacacg attgagccaa ctttcgtcat
    13801 tcgtatgcta tcgactacat ctttactgcc ttttatttta aatacttgca cgtcatattc
    13861 aaacggcaca tacgcattgc ctaacttata acttttctta tcagcgctat attccacagc
    13921 aaaattatta atgacatcac gctctgaatt agtaacgctc catgcaatgc tgtcgttgtg
    13981 tccgataaca attccaggcg caccaggaat ggtaacacct tcaacattta tcgttggaca
    14041 atgcagttga atggcatacc aaattgaagg taagttcagt gctaaatgtg gatcgccaca
    14101 caatatcggc tttcctgatt ttgttttgtt tccatttata caccaattat tactgccata
    14161 aaatttattg gcatataacg ctttttgcgg attgctatat aataatgcag tctttgtcgt
    14221 tgctgtattt tttgaagaaa aagtatcgac agatgtaaaa ttccattttg tattaacagg
    14281 aataattgga tcaagagcag gtggcggaaa atcaggatac atttttttat atacttcttc
    14341 gccaaaggct cgtaaagcat ttgtattttc gatgtctgtg tcgtaatagg tcaagtcttt
    14401 cgccatgtat ttcatgagca aagcacattt taatggcgac catgcttctg gttgatagcc
    14461 aagcaattta tattctattg gataatctgc tttttttaat tgtttgatat aagcattgac
    14521 gccagcagaa aaactttcca taattttttt acttatcgga tctttttcca tttcttttac
    14581 agaattatgt gcagcgcgca gtatgcctat ccttcttgaa aatctatcag catctaaaac
    14641 ggaatgaccg ctgatgattc tgtttcctaa tatttctgtt aatcttccag cagcagcatg
    14701 tgtttgaaat tccatttgcc acaatctatc tttcgcttct acataacctt gcgcatacac
    14761 taagtcatct tcgttgtcgg caaaaatatg cggtatgttg tattcatcat aaacaacagt
    14821 agcctcgccg tttatacatt ttattacgat acttgatgtt ttattattat ctgtgctagc
    14881 attttgccaa aaaccatgtg taggtgaaaa aaattgccca agagcaggta actcacccat
    14941 tggcttgttt agaaaaaaaa taccaatcgc tacaattata gcaatcaata ttcctgatac
    15001 tttctttatc ataccttttt tatgtttatc taatttacat aaaagttgtt aaggaaaagc
    15061 ttttttcctc taaattcaat ttggcattgt ttatttttga tgtatgacaa ataatgagat
    15121 agcaaagcat ttttctttac tcgctgattt aatggaattg cacaatgaaa atgcatttaa
    15181 aattaaaagc tatgcttttg caggacgtac attaaataaa agaaatgaag cattgtcaac
    15241 attaacttta gatgagctag aaaatgtaga aggcatagga aaagcaatag ctgcaaaaat
    15301 tctacaacta acacaaacag gaaagcttga tttattagaa aattatttag caaaaacacc
    15361 aataggcgtc gtcgaaatgc ttcagttaaa aggaattggt ccaaagaaaa tagcacaact
    15421 ttggaaagaa cttgagattg aaagtatagg cgagttagaa tatgcttgtc aagaaaatag
    15481 attggtagaa ttaaaaggat ttggcactaa aacacaagaa aatattttac aacaaatcca
    15541 atttttacaa caaaatgcgc ataaattttt atgggcaaat ctcgaagagt tagccaatga
    15601 gattttagca gaaattcaaa atgcttatcc tgaaatatta gtatcaacct gtggtgatta
    15661 tagaagaaaa tcaatcattg ttgaacgtgt agattttatt attgccacca cgcaaaatga
    15721 aatacaagaa gctttagttg aaaaatacaa acagtatcca gtgcattttt atttctgtga
    15781 taccaagcat ttttattgga atttattgca attgtcgagc gataaagaac atgcaggatt
    15841 tttatcattc aaaatagata ccaataaatc ctttacttct gaaaaagaaa tttatgtaga
    15901 agctggcttt ccattcatcg cgccagaact acgcgataat aagtatgaat ggaaattaat
    15961 agaagaaaat aatttacatc atctcgtcga actccaagat atcaaaggca ttgttcatac
    16021 acatactaaa tttagtgatg gtgcaaactc tttagcagaa atggcaagct attgcaaaga
    16081 acaaggcttt gagtatgtag tgatttcaga ccatagcaaa agcgcatttt atgcaaatgg
    16141 catgcaggag gaacgcatct ttcagcaaca tcgtgaaata gataaattaa acgagcagct
    16201 tgctccattt aaaatattta aaagcataga atcagatata ttatacgacg gaaatttaga
    16261 ttatgatgac gatatattat caacttttga tttgataatt gcttctgttc attctcaatt
    16321 aaaaatgaac gaagaacgtg ccatgctgcg attgattaaa gcgattgaaa atccatatac
    16381 gaccatctta ggacatctta ctggcagatt gttattgtcg cgaaacggct atccaataga
    16441 ccatcaaaaa attatcgatg catgtgcagc aaacaaagtg agtatcgagc tgaatgcaaa
    16501 tccatatcgt ttggatatcg actataaatg gattgcatat tgtcaagaaa aaaatgtacc
    16561 tatatgtatt aatccagatg cacataactt acgcggtgtg cacgatatac gttatggtgt
    16621 gtttgctgca agaaaaggtg gacttttgaa ggcaaatacc ttgaatacgt taacgaaaga
    16681 taattttgaa aaatacctat cacatactaa acgctgatgc tattttagta tcttcgcctg
    16741 aaattttttg acatgaaaaa atgctttttg tttttagttt ttattgcctt tgcttccttt
    16801 gtttttgtac aatgtaaatt taaagacgac aaaaatgaaa taattgtaga gactttcaaa
    16861 catgattcta ttttggctaa taatccagtt ttgaaaaaag cagattcaat ggctaaaata
    16921 acagcacaaa ctgaggaaga aagactgaaa acggtgacct ctattaagtt tgaaaaagaa
    16981 gtgtatgatt tcggaacgtg taatgaaggc gataaaatca cgagagtaat tgaatttacc
    17041 aacactggaa aattaccatt agtgattaat caagcttatg gaagttgtgg ctgcacggtg
    17101 ccaaagtatg acaaagagcc tgtaatgcca ggtaaaaaag gtaaattgtc tgtagaattc
    17161 gattcaaaaa acaaacctgg tgctaacaca aaaagtatta tgatagaggc aaatacaaat
    17221 ccacccataa ctactgttgc ctttagcgtg aaagtgaaag aatcgaaatc gaaaaaatct
    17281 tggtttaaat aataaatcca tgaaaaacta tctttcttta gttaagtttt cacacacagt
    17341 ctttgcatta ccatttgcaa tgattggttt tacattaggc atctattatt tttttgcttt
    17401 atataaatta gccaatcctt ttgatgcgtt ccagccagca tttttattaa atacatacac
    17461 aaaatatcca ttgccttttt tcattattta taaattagta tttgtgttaa tttgcatggt
    17521 tactgctcgt aatgcggcaa tggcatttaa tcgttacatc gatagaaaat acgatgaaaa
    17581 aaatccgaga accgctattc gtgaaattcc ggcaggcatc atcacctcta aaaatgcaat
    17641 gatttttatt gttatcaatt gcatactatt tgttgtagct acatattgta ttaatacact
    17701 ttgcttttat ttatcgccaa ttgcattgtt agtcatttta ggctattcac tcaccaaaag
    17761 atttaccttt ttatgtcatt taattctagg tgttggattg ggcttagcac caataggcgc
    17821 atttttggct gttacaggca agttcgaaat tttgccattg tttttctcgt tcgcagtact
    17881 attttgggta gccggttttg acatcattta tgctttgcaa gacgaacaat ttgacaaaga
    17941 aaataaactg tattcaattc catcatattt tggtcaagat aaggctaagt ggatcgcacg
    18001 tttattacat gttgctagta ttttatgttt gcttggcgca ggaatttatg gcaattttgt
    18061 atttacttat tggctaggat ttgccgtgtt tgttgctttg ttgttttatc agcatttttt
    18121 agtgttgcaa tatggtttag aaaaaataga ccttgctttt tttacaacga atggtattgc
    18181 aagtattctg ttttctctat gcgtaattac cgaaattatt atgcatattt aatcatacat
    18241 ttaagagcca ctttattttt ataatcaata atgtcgaaaa ttttagccat agattatggt
    18301 gccaaacgca caggatttgc tatttcagat gaaagcaaaa tatttgcatt tggactttcc
    18361 acacaagaca ccaaaaatgc acttcaatat atcggacaaa tcattcctaa agaaaaaatt
    18421 gatacaatcg ttatcggaat tcccaaaaaa ttaaataatg agcatgcatt gctagtgcca
    18481 caaatacaat cgtttattaa aaaaataacg caacagtttc cagacataca aatagttgaa
    18541 atcgatgaaa gatttacttc gaaaatggca tttcaatcga tgatagacag cggcttgaaa
    18601 aagaaagacc gacaaaataa agctttaatc gacgaaattt ccgcaactat catcttgcaa
    18661 aattatctcg aacaacaata gcttgtaata ttttactaac tttgcacaac ttattcattc
    18721 agattatctt ttaattaatc aataaacatg ctacttccag taacattata cggcgaccca
    18781 gttttgaaaa gaaaagcaga tgaaattgct gcagattatc ctaacctaaa ggatatgata
    18841 aaaaatatgt tccaaacaat gtatgccgca tctggtgtcg gaattgcagc gccacaagta
    18901 ggtgtttcta ttcgtgtttt tgtcgtggat accatgcagc tcgatgaaaa acgcaaatca
    18961 tcacccaata caggcatcaa aaaagcattt atcaatgcaa ctattttaga agaaacaggc
    19021 gaggagtgga aatacgaaga aggctgctta agcattccag gtatacgcga agatgtgtac
    19081 agaaatgcac aagttaaaat aaagtatttc gatgaagagt tcaacgaaat tatcgaaacc
    19141 tacgatggca ttaacgcacg tgtcatccaa catgaatatg atcacatcga aggcattttg
    19201 tttacagata aattaaaacc cttaaagcgc aaattactca ttcctaaatt aaataaaata
    19261 gcacgcggcg aggtcgatgt cgattatcgt gttaaagcat ataaaaaata ggaaaatcct
    19321 attttttctt cttcttattt ttaatatctt ttataaaacg agcccaagca aacggatccg
    19381 tcaatgaaat accaggtctt tgattaccat atctattata gtagtagcct gcctgcgact
    19441 gcacatattg attgaaattt tccttaccat caaaattcgt ttttgacgaa atctcatcat
    19501 ttaatatcga attttctatg gtttgctgtg ccaagtcgcg caaattgtca ggaatgtcta
    19561 aattcaatac cgcataacgc aacttttcag gcgtaggcaa gggcctcacc gtcacgcctt
    19621 taagctcata tgtttccctt tccaaactca catctttcac aaaacttgta agctctaagt
    19681 cttttggaat cgctatttta aaagacttgt agcctaatga tttcaccaaa atagtatcgc
    19741 cttttcctgc cgcaaaagta aaaaatccat cgtaaccagt aatcgttcct cgattttcat
    19801 tttgaatata caccgcacaa aacggaatca cagcattgct ttctgcatcg cgcacaaaac
    19861 ctgaaaattg gatgatatct ttcgaaaatg caacaacact cagcaggcaa ttcaacacca
    19921 aaaacagata taattttttc atcatcataa acttccaaaa ataataccaa ttattggtta
    19981 atactactat ttacaatatt ataatgtttt ttaacagatt ttggcgtgcc ctcgcttagg
    20041 cgaggtcagg ctgtccgcta caatcttttt tttgccacgc tacaaaatac ctatgctcaa
    20101 aaaagtattt ccgcttccat ccttcacgca ttacaacaaa tcacgtatct ttagcactca
    20161 tttttaattc tatcatatgc aatatatcaa agaaagtata gcgctgaata aagaacgctt
    20221 cgtagaagaa cttatagact ggttgaaaat tccatctgtc tccgctgatc caaaattcaa
    20281 agatgaggtc ataaaagcag cagattattt aaaaacacag tttgaaaaaa tcggcatgga
    20341 taacgctcaa ctgctaccta cagctggtta tccagtggta tatgccgata aaattaccga
    20401 tataaataaa ccgacagttt tagtatatgg ccattatgat gtgcaacctg ccgatcctta
    20461 tgaattatgg acttctccac catttgagcc cgtcgtaaaa acaacagctg tgcatccaca
    20521 aggagccatc tttgctcgtg gcgcctgcga tgataaaggt caagtatata tgcatgtcaa
    20581 agcattagaa atgatgttat ccaataatga tttaccgtgc aatatcaaag tgattataga
    20641 aggagaagaa gaggtaggct cggcgcattt aggcgatttt ctaaaagcat ataaagaaaa
    20701 actaagtgct gatgtaattc ttatttcaga tacaggcatt ataaataatg aaacaccagc
    20761 aatcactaat ggtttgcgtg gcttgtgcta tatggaagtt gaagttgtag gcccaaatcg
    20821 cgatttgcac tcaggtattt acggcggtgg cgtggataat ccattaaatg tgttatgcaa
    20881 attaataggt tctttgaaag atgataatgg tcacattacg gtagaaggtt tttatggcga
    20941 tgtaatagaa ttaagcgaag aagagaaaaa acaaattaat gcagtgccag taacagagag
    21001 tgagttcgaa caaagcattg gcttgcctaa gtcatttggc gaacaaggct ataccttatt
    21061 agaacaattg agtattcgac ctacattaga tgtaaacgga atttggggtg gctatatagg
    21121 tgaaggcgcc aaaacagtat tgccatcaaa agcaaatgca aaaataagta tgcgattagt
    21181 tcctaatcaa tcatctaaaa aaatcgcaca attatttgcc gagcatttcc ataaaatttc
    21241 acccaataca gtgaaagtta ccgttaccga acaccatggc ggtgagccgt atgtaacacc
    21301 tgttgactcg gtcgaatttc tagcagccta caaggcgatg gaaacgacat tcggcaaaaa
    21361 acctttggcg atgcgcaatg gtgcaagtat tccaatagtg gcattattcg aagatatttt
    21421 aggactaaaa tcagtgttga tgggcttcgg attagatagc gatgctattc attcacctaa
    21481 tgaacattat ggcttattca actatttcaa aggcattgaa acgattccgt atttttataa
    21541 atattatgcg gagttgaaac aattgtagaa aggaataatt taattttttt ttgtaatttt
    21601 aaaatatgat ttcaataaaa gaagatatta ttaccaaaat tcaaaaagca gacgatgcac
    21661 agttattggc agaaattcat agaattttag atgccaatga ctatattttt acggctgaag
    21721 atgttgcctc tttgagaaaa gcggatgaag agatagataa tggaaattat aaaacacaac
    21781 agcaggtaaa aagcaatatc caacaatgga taaacaattt gaaataatct ggtcgccaca
    21841 agctgaatta gatttacaca atattttatt attttggttg gaacacaata aatctattac
    21901 ttttcctgaa aagctataca atgaaatttt ggaaacaacc gaaagtttag ttcaaaatcc
    21961 atacatagga aaaaatatac aaatagaaga ttacagaaga attttagtca ggtattattt
    22021 aatcgtttac agagtagaaa aaacagaaat aagaatatta agaattttgg acggccggca
    22081 aaaggaattg aaagcgtaaa atgcatcgag acgattccat atttttacaa gtattatgca
    22141 gaattaaaat agcataaaaa taacaatcga aaaacaagtt taataggttt gataactcga
    22201 gacaccatag caaaaatttt agatacggct cagattgaag acgtagtgcg tgattttgtc
    22261 aatctcaaaa aaacaggttc tatactcaaa gggaattgtc catttcatca agaaaaaacg
    22321 ccatcgtttg tggtgaatcc gaataaaaac atttttaaat gttttggctg tggcgttggt
    22381 ggcgatagcg taagttttat tatggaacac gaaaaatttt cgtttccaga agcattgcgc
    22441 tatctcgcac aaaaatatca tatcgaaatt gaagaaacac atgtttccaa tgaagacaaa
    22501 caagtgcaag atgaaaagga aagcctgctg attgcattaa attatgctgc taagttttat
    22561 caaacacaat tgttcgaaac agaaactggc gaagccattg gattgtccta ttttaaagaa
    22621 cgtggctttt tagaagagac tattcgcacc tttcaattag gctactcgca agatagcttt
    22681 gatagttttt acaaacaagc cattgcagat ggttataatc ctgaagtatt agccaaagca
    22741 ggattgataa aagaaaaaaa tgataaacat tacgactttt tcagagacag agtgatgttt
    22801 ccgatacaca atgtgtctgg aaaagtagtc gcatttggtg gcagaatttt aaaatcaaat
    22861 aaaacagaaa ccaacacaag tgtatataat ccgaaatata tcaatactgc ggagactgat
    22921 gtttatcata aaagccaagt gttatatggc atttcacacg ccaaagcgga cattcgtaaa
    22981 aacgacgttt gttatttagt ggaaggctat acggatgtga tttccttaca tcaagcaggc
    23041 atcaaaaatg tagtggcatc aagcggaact gcattgacga aagagcaagt gcaactcatc
    23101 aaacgtttta cacaaaacat ggtgatcttg tatgacggcg atgcagcagg cattaaagca
    23161 gcaattcgtg gcttagatat cgtgctcgaa cagggtttga atgtaaaact ggtgctattg
    23221 cctgatggtg aagatcctga ctcttttgta tctaataatg gaactgattt tacattagat
    23281 tttattgcac gcaaacaaca agattttatc ttgtttaaag catcacaagg cattgaagaa
    23341 gccaaaaatg atcctgttag gaaaacagaa attattaagg atatcattag cagtatagcg
    23401 aaagtagatg ataccatcaa acgccagttg tatatcaaac aatgtgctac aattgtcgat
    23461 attccagaaa atattttaac taatgaaacg aataaaattc gtacacagct atttagaaaa
    23521 aatattgaaa tttcgcaaga agatgcaacg aatttaatca aacatgtaag tgcagagatt
    23581 gaccaccatc agcaagtgtc aaaaacacca cacttgtact ttcaagagcg tgatattatt
    23641 cgcattttga tagaacacgg cgataaagaa atgactgaag aacattctgt tgccgaatat
    23701 gtgatttttg agttggttga tatcgaattc aataatgcaa cttttgggaa aattataaaa
    23761 ttatttttag atgcttatca aaacgatatt ccgttcgata aactcaaaga tataagcacc
    23821 atcgaagaca aagaggttaa agattgtctt atcgaattga tgtcctcacc atacgaactg
    23881 agcccgaatt ggttcgacaa acatgaaatt gtcgttaaag acgtaaacag aacttttaaa
    23941 acagatgtcg tttctgtgat gttgcgtttc cgatatttca aattgatgga agccattcaa
    24001 atgatagatg caaaaattaa atcatcagaa gaaaatggac agtttgaaga aatgttggcc
    24061 actatgatga aaaaaatgga atataacgat gagaaaaaga aattagccaa ggaaatccaa
    24121 accgttattc acccatttta ggcagattcg tcttaaatac tattaacaaa tagcgaattc
    24181 aaacaaatac ggaacgaatt tcgtaacttc gcggtttaat ttgtaaaaaa aacaaagtat
    24241 ttagcgtatt ctcatttttc aaataataaa ccttcaatta atatatgtta ttattagcca
    24301 taagcggaac agcaactaaa gttatattac ttattttaag cttatccata ttagtagttt
    24361 tacatgaatt aggtcacttt attccagcaa aactatttgg tactaaagtg cataaatttt
    24421 atctattttt cgatttctta tttccatttc ccaatattgc taatttcagt ctattcaaaa
    24481 agaaaatagg cgacacagaa tatggcatcg gctggtttcc atttggcgga tatgtccaaa
    24541 ttgctggtat ggcagatgag acacaagatg ctgaagaact aaaaaactca acgccacagc
    24601 cttgggaatt tcgtagcaaa ccagcttggc aaagattaat tattatgatt ggaggcgtaa
    24661 ctgtaaatat ggttttagcg atgattattt ttacaggctt actttggaaa tatggcgaag
    24721 aatatttgcc attcaaaaac atgaccaatg gtatcatggt ggtagattca ttggcttatg
    24781 aagtaggatt gcaaaatggc gataaaatta ttgctataga ccatcagcca gcaggtaatt
    24841 tcgaagaatt tcaaaaagat ttagtgctga acgaagccaa aaatatcacg atagaaagaa
    24901 atggacaaca acaagacata cagtttaaag atggattttt gtctaagttg atgaaacaga
    24961 aaaaagccac tttcgtcttg ccacgcgtac caagcatcgt tgcgcaagta caagatggtt
    25021 ctggagcagc taaggcaggt ttgaaaattg gcgataaaat aactgcagtc aataatgtgc
    25081 caacgccttt ctacgaccaa gtaaagaatg aaatcacagc caataaaggc aaacaaattg
    25141 caatgacagt agagcgcaac ggcaaaccac ttgttttgca gggcactgtt tcgcctgaag
    25201 gtttgtttgg cttcggcaca caattatatt cagaacttct aaaaacagat agagtaaatt
    25261 ataattttgc agcagcggtg cctgctggta tcgaaaaatc gattagcaca ctggctaatt
    25321 atttcaaaag cttgaaaatg ctattcactt caaaagaagt aaaagtgaaa gatagcttag
    25381 gtggttttat ttccatggga aaaatgtttc ctgacacttt tgattgggag cacttttgga
    25441 tattaacagc aacaatctct gtgattttgg catttatgaa tattctaccg attccaatgt
    25501 tagatggtgg ttatgttgtg tttttattgt atgaaatgct acgcggaaaa ccagcaccag
    25561 aaaaggtaca acaagtagca caaagcatag gtatgttcat cattttaggt ttattgctgt
    25621 ttgccaatgg tatggatttt ataagagcaa tttttaatta aaatattttg tcaaacatag
    25681 ttgtgaattt gcttttttca ctatctttgc caaccgttct tttaaccttt aataaaacga
    25741 cacatttaat gatgtagttt taattaatac cagccgaagt ggcggaactg gtagacgcgc
    25801 acgactcaaa ctcgtgtacg caagtgtgtg ggttcgagtc ccaccttcgg tactaaaaaa
    25861 tgcaaccttt gtgttgcatt ttttttattt ttttagttca aaattatcac tcaaaattta
    25921 tcatactttt tcgtatttag ccaatcttat aaaagtataa tacaagcata aatatgtgta
    25981 aattaataga attaagacct ctacttcact aaaatcgctt acttacattt cttttgagtt
    26041 aaatattgag cattaaacta aaaaatatta aatctatttt gtaatttaat gtcgattttg
    26101 aataatcata acatttatcc aaccaaaaac aacgaaaaat gaaaaaatta ttactggttt
    26161 tattgcttct tggagtaata gtacatgagt cagatgcaaa aattcaagct ggcgtaggca
    26221 tcaaagtagg tcctgcaatg ggcagatgga ataatggcgt tttgaatccc aaactatcta
    26281 cagcaggcgg tacagtaggc ttccaacctg gtgtgcatgc aggtattcaa gcgagaatat
    26341 ggttcaataa atttgtaggt attaatttag ctggagaatt taatatgcaa ggtagcacat
    26401 ttacacgtgt gtcaggtgta aatagccaaa aagtaactca taaagaaaac caaataacat
    26461 tgcctttgtc tgtaatggtt ggttggggaa atgaacgctt acgtgttttt ggtaacttcg
    26521 gcggatattt tggttataat attaatggca aagacaaaga agcgatttac ttaaatggca
    26581 gcgagcaacc taacagtggt tttacaaaag ctgattataa agaaaaatat aatagcatcg
    26641 atgttggtgt gcgtgtaggt ggtggtatcc aagtgtatgt tgacaaaaaa ttgaaaagct
    26701 gtgttacttt cgatatcaac tatgattatg gcttgatgaa aacatttaga gaggaagaac
    26761 cttcttattt ctctaatcca gagaaaataa aaataactcc ttctaaattg ttaattggtg
    26821 ttggatattc ttatacattt ggcaaaagcc aagcagaaga aaaaccaaga agagcaggtg
    26881 atatgattac agactaattc agaaagtaat agatatgtat ataaaaaagg gaaagcttaa
    26941 gctttccctt ttttatgtaa tgtagcataa tcttttgcgc cataacttat aattacatcg
    27001 gcaccagcac gatgaatact taataaggtt tcagccaaac atttatcgta atcgagccag
    27061 ccatttttag cagcagcttg caacatagca tactcgccac taacgtgata tgcagcaatt
    27121 ggaagtggaa aattttgttt caaatcttgt ataatatcta aataaagtgt tgcaggtttc
    27181 accatcataa aatcagcacc ttctgtatag tcgagttcgg cttcaattaa ggcttctcgc
    27241 ttattgcccg gattcatttg gtaagttttc ttatcgccag acttaggcgc agagtctaaa
    27301 gcatctctga atggaccata aaatgcactt gcatattttg ccgtgtaaga catgatagaa
    27361 gtatccgtaa aaccttttaa gtctaattgc tcgcgaatgt atgcaacgcg gccgtccatc
    27421 atgtcgcttg gtccaagaat atcgatgcct gcttctgctt gtgccaatga catttttgct
    27481 aaaattggca atgttttatc attgataatt ttaccatttt caacataacc atcatgacca
    27541 tctgaagagt atggatccat ggcaacatca ctaattaaac atgattcagg aaatttcttt
    27601 ttaatttctt ttattgcttt taaataaaaa tttttagaag aataactata agtagctgtt
    27661 ttatttttaa gcgcatcttc aactgcggga aatagaataa agctatttac acctacttgc
    27721 atgcatgctt cgatttcttt taaagcatta tcaatgctga gttggtgaat atttgttagt
    27781 gacttgatgg catttttttt cttttggcca tctaccaaaa ataatggttg tactaaatga
    27841 ttaacatgaa gtttggtttc gatagttaaa cctcgtatag cttcactttt tcggtttctt
    27901 cgtggacgtt gtaacatgca gcaaagttac tacttaaggc ctaatttctc caatgaaagc
    27961 ctaatttttt gctccgtgtc gtcagtgtca gctttttcaa attttttacc taaaataagc
    28021 tcgtacaatt cgatatatct attcgaaata ttttgaacaa tttcgtcagt catttcagga
    28081 acagtttgcc cttctttgcc catgaaatta ttgtcaatta accactcgcg tacaaattct
    28141 ttggaaagtt gtttttgagg ttcattgttt tttaatcgtg tttcaaatcc atcagcataa
    28201 aaatagcgcg aagaatctgg tgtatgtatt tcatcgatta agtaaatagt atcgccaatt
    28261 tttccaaatt catatttagt atcaaccaat agcaaacctt gctttgcagc tatttctgtg
    28321 ccacgttgaa atacttgttg tgtatagtgt tctaattgtt cataatctga cttggaaaca
    28381 ataccgcgtg caatgatttc ttctttagat atttcttcat catgacctac caccgctttt
    28441 gtagttggtg taataatcgg tgtgtcaaat ttttgatttt ctatcatgcc ttctggcaat
    28501 gcaactccac ataatgttcg ttctccattt ttatatgctc tccatgccga cccagcaagg
    28561 taaccgcgaa taaccatttc tacgcgaaac ggttcgcaaa cttttccaat cgtaacatta
    28621 ggtgctggag tgtcaatcac ccaattggga acgatgtcac ttgtttgtgc taaaaaatta
    28681 gctgcaattt ggttcagcac ttgtccttta tatggaattg gttttggcaa tacgacgtca
    28741 aatgctgaaa ttcgattgct aacaatcatt accaaatact gatcatcaat attatataca
    28801 tctcgtacct ttcctttata aaaactcttt tgatgtggaa aatgaaatgg tgcttccatg
    28861 gaagtattta ttttttacaa aaatacgcta aagcaaatgg aaatacataa gtatgtttcc
    28921 acaggtatag ttttgttaac agcaaataaa agattgtttt ttcacagcag gctattttgt
    28981 ttaaaacttg ccattatatt ccatttaatt gcttttagaa atttgtacat ttgtacacca
    29041 tggaaaattt catcgtttct gctcgcaaat atcgtcctgc taaattctta gatgtagtag
    29101 ggcaagagca agtttctgaa acgctgaaaa aagcattgtc cagcggcaaa ttagcacaat
    29161 catttttatt ttgcggacca cgtggtgtag gtaaaacaac gtgtgctcgt attttagcga
    29221 aggcgataaa ttgcgaaaat ccaggagccg attttgaacc ttgcaatcaa tgtacttcgt
    29281 gtgtgtcatt ttcccaaaat gcttctttta atatattcga attagatgca gcatcaaaca
    29341 atagtgttga tgatattcga actttagttg accaagttag atttgcacca caaagtggaa
    29401 aatataaagt gtatatcatc gatgaggtgc atatgttatc gcctggtgcc tttaatgcct
    29461 ttttaaaaac attagaagag ccaccaagct acgccatttt cattttagcg actacagaaa
    29521 agcataaaat tattccaaca attttatcgc gctgtcaaat atttgatttc aaaagaattt
    29581 cggataaaga tattgttagg caattgcaga aaatttgcga tagtgaaggt gttcaagctg
    29641 aaccatcagc attacatatc attgctcaga aagcagatgg tggattgcgt gatgctttat
    29701 cgctgttcga taaaatagtg agctatgcag gtaagcaagt cagctatcaa gatgtaattt
    29761 ctaatctcaa tattttggat tatgaatact atttcaaaat taccaatttc tttatgttgg
    29821 aagacgcggc aagtgttttg aatgctttca atgaaattat taataatggc ttcgatggtg
    29881 aaatgttttt gttaggcatg gcagaacatt ttagaaattt gctcatcagt aaagatgaaa
    29941 aaacagtgcc attattggaa gctgcagaag atgtaaaagc aaaatattta gctcaagcaa
    30001 aattgcttaa tgaaacattt ttactcaatg ctttaagtat tatcaatcaa tgtgatattg
    30061 aatataagac aacaaaaaat aaacgcctac atgtagagtt gtcattgttg aaaatttgtt
    30121 atttgaatac acaaatagaa agtacaatct cattgatttt aggtgaaaaa aaaaactaaa
    30181 atcacttctt cagttttata agcaacagaa acaacaacag gttgatgtgc aattagaaga
    30241 tgaatctgta aatagtttac atactaaaac gattcaagct gtaattgctg atacaaacgt
    30301 gctaaaacaa cacgtagaca atacgactac ttcgcaagaa aataaacaac aagctacact
    30361 tgaacgcaca gaattaaata caaatgctat tgcatctgta acggcccaaa aatcaaaacg
    30421 cgaactgcca aaaacctcga gcattggtag tatgtttaaa aatttagaaa aacaaaatgt
    30481 cgaaaaaaaa gtagaggcaa aactggaact tacaccagaa aatacaattg taatttggaa
    30541 taaatttttg aatgaaaata aggcaaactt gcacagttca tttttaagcg cagcgcaaag
    30601 ccatccacca gttttggaag atgataaaat tgtatttaca gcatcgaata atgttacctt
    30661 agaaatgtta caattgcaca agatggacat tactgctttt tttagattgc gtacaacttc
    30721 agcatcggta gtgcctgtgt ttgtcctaaa tcgcgccgaa tcaccaatta aagattataa
    30781 gactaacaaa gatcgtttaa aagatatgat tgattcgaat gcctccatta tgaaattaat
    30841 tgaaaaattc gatttaaata tggaatagaa attagaagat attatgaata aaaaaacagt
    30901 agtgataggt gccagcgcaa aacaggatag atacagcaat aaagccgtac gtatgttgca
    30961 atatcaccat catgatgtga ttgcacttgg ttttgaaaat gcgcaaatta atgatatttc
    31021 tatacaaact atatggcagc attacgataa tgttcataca atatctttgt atttgaatcc
    31081 acaaagacaa aaagaatatt attcctatat tttatccttg cagccaaaac gcattatttt
    31141 taatcctggt acagaaaatg acgaactgca acaattagca acacagcaaa atattgacac
    31201 attggaagcg tgtacactag taatgctaac aaccgagcaa tattaatttt tgttattttt
    31261 ataatgccat taaaccttgg cacaattttt atactctaat gataaaataa atgtcaatga
    31321 aaaaacaaat tctaatgctg tttgccgtcg tgtttagttt cacggcgatg agctttgcac
    31381 aaggaaatcc gaatgctaaa atgaaacctg tgaaattgca aaaagtatct gttgaacaga
    31441 aagcaatcaa tgcaacagat acaatgcaaa aagtagctca attaagtaag gaacaatacc
    31501 agcaaatatt ggcaataaat caggattttt tcaaacagca acacactata aaatcagaaa
    31561 tgcgtaaaga tacttctgca aatgatatcg catttaaaga acaattaata caattatcaa
    31621 aatcaagaaa agcgagcatc aataaactgc tcacggaaac acaaaagact gtatggaaag
    31681 attataaaaa aatcaacatg ccaaacaaga aaaataaatc atcaaaacaa gatatgttta
    31741 tagatgatga aagtcttgat gaaatgtaaa aaatattgat gtgttaaatg ccttacaaaa
    31801 ctgtaaggca tttttgtttt aaacttttac aatttttata agtctaatta ttatgcttac
    31861 ggaacaagat aaaataaaac atctttacaa cagagctggt tttggctatg ctttgagcga
    31921 tatccaaagt ggtagatctt tgtcaacgac gatagaaaaa ttattttcta ataaaacata
    31981 tcgaagttta caaacagtta catttactga agtagaatcg gcaaaagaaa aaatgaaatc
    32041 atcgccaaaa gatgccgcta aagaaattaa aaaattattg aaagacaata tctctgattt
    32101 gaataattta tggtttcaag aaatgattgt aagcgatgca caactacaag aaaaaatggc
    32161 attattctgg catagtcatt tcgcctgtcg ttctgtaaat ccatattttg atcagcaata
    32221 tttagatatc attcgcaaac acgctttagg tgatttttca acacttctct tcgagatttc
    32281 caaaacacct gccatgttac aatttttgaa taatcaacaa aataaaaaag atcatcctaa
    32341 tgagaatttt gctagagaag tgatggaact ttttacatta ggtagaggca attatacgga
    32401 aacagatatt aaagaagcag cgcgcgcatt tacaggctat ggatttgata aagtcggaag
    32461 ttttaaattc aagaaacaat tgcatgattt tggcgtaaaa acatttcaag gaaaaacagg
    32521 aaattttaat ggtgacgata ttttaaaaat aatactaggc aataaaaact gttcaacatt
    32581 tcttgttaga aaaatttata gttttttcgt caatgagcaa gtagatgaac aaatagttca
    32641 atctttatcg gcgcagtttt ttcaatctaa ttacaatatc aaacaactga tgaaaactat
    32701 attttcatca tcatggtttt atgatgaaaa aaatattgga aataaaataa aatctccaat
    32761 agtattatta acaggcatgt ttcgcttggt gcctacaaca tttacaaagc ctgagaatgt
    32821 attatttcta caacgctcac ttggccaggt gctactcttt ccgccaaacg ttgctggttg
    32881 gaaagaaggc aaaacttgga tagatagctc ttctttatta ttcagaatgc aactaccaca
    32941 aattatttat tttgataaat ctttagacat ggaaccgaaa gaagaaattc cggaaatgta
    33001 tgcagcaatg caaaaaatta aaagtgatta catgaaaagt tttatcgcaa aaaaaataag
    33061 tgtagcatct gattggacag caatcattag tcattttatg ttgagtaaat cagtgacaaa
    33121 tgatgctgct tattttgttt tacaacagcc agcagatgca atgactttaa aagcagtcgt
    33181 agcaaattct gatttttcat caaaacaaaa tgaaataaaa agtacgatta tcaatatgat
    33241 gtccttgcct gaatatcaac tttgttaagc aaaaacttgt aaacacatga ccatgaaaag
    33301 aagaacattc ttacaaacat caggcttagc aacaatgtca atgctagtgc caaaattttt
    33361 gaaagctaga gaaaatataa attcaattag ctcaaataaa atattggttg tcgtgcagct
    33421 gagcggtggc aatgatggct taaacacagt tgttccttat gaaaatgatt tatattatca
    33481 agcacgacca agcattgcca ttaagaaaag cgaagtactg aaattagata cactactcgg
    33541 ccttcatcca aacctgcatg gatttaaaaa actatatgac gcaggcaata tgtgtttgat
    33601 taataacgtt ggctatccag atcctgatcg ctctcatttt cgctcgactg atatttggca
    33661 aacggccagt aatagcaatg aatacttgag cactggctgg ctgggcagat atttagacgt
    33721 tgtatgtaat ccgtgcgaac gaccaactca agccatagaa attgatgata ctttaagttt
    33781 agcacttaaa ggtacacaag caaaaggatt agcttttaaa gatcctaaaa aatggtataa
    33841 cacaactaca gataatttct tcaaattatt atcgaaacaa cattcagaag cgcatgaaca
    33901 cgatactgca ggttatttgt ataaaacact ttcagaaacc atttcctctg cttcttatat
    33961 ttataacaca tctaaaattt atcaatcatc atctgtgttt ccaaatcatg catttggcaa
    34021 aaatttaaaa acaattgctg agttaatcgt gtcaggcgta caaacacaag tgtattatgt
    34081 taccttaaca ggctttgata cacataataa tcaacagaaa agacaaggtg ttctgcttca
    34141 acaattggca gatactattg atgttttctt taatgactta aaaaaatctg gcagagccga
    34201 cgatgtgtta ttaatgacat tttcagaatt tggcagacgt gtacaagaga atgcaagcaa
    34261 tggtactgac catggcactg ctaatcaaat tttcttattt ggaaataaac tgaaaaaaca
    34321 aggcatttat aatgaagcgc caaatttatc atcactcgat gatggcgatt taaaatatac
    34381 agttgacttt agaaatgtgt atgcaacaat tttacataat tggctgcaaa caaatgaaca
    34441 tttaattcta tctaattcac catcattatt gaattttatt taacgctatt ttgcacatct
    34501 gttagctatt ccatctgaat acttcgtcat taacttgtta tttttgaaga caagtttagt
    34561 tatgaaaaaa aatccgttta gcttttgcac tttctttatg ctttgtgcaa tgatatcact
    34621 tcatttttct gtttcagctc aagatgttat tgaaattgtt gatccgtatg aaatgaaagc
    34681 gtcaagaagt aaagaggcag atacatttta ttttacacaa aatacaaatg ctatacttaa
    34741 gaaaaaactg gacaccttac gaacgtatga tgtcttttta gaagaaagat taacgccttt
    34801 tggaaaggct tatatgtgta atggaaatga agtaacaaaa cagaaatatt tagaatttaa
    34861 aagattttgg ggcgcttcgg cagcatgtac accttgcttg ctttacacat acaatgataa
    34921 aaatgagttg aagtatgttg cttatcaata cgaagaatgt ttatgcggaa gctataaaga
    34981 atattataca gatggtactt taaaagtaga aggtcagttt aagcaaaata ctacaggaag
    35041 ttgggaaaat ttaaaatcaa aaaatgtgtg taacgtgcgc gatggtattt ggatttacta
    35101 cactgaaact gccgtagaag aaaaacgaga aacatatatc gatggcaaat tgaaaggtac
    35161 tacattctca ccatcacaaa catcacctgt caaacaacaa gcagccaaac aaattgaaga
    35221 ttccactcaa acgaatgaac ctaaaaagaa ttggtttaaa aaattaaaag acaaaaacac
    35281 agacgagcaa taagcactta gtagaatagg tgctattcca attgcatttg gtctttattc
    35341 tctctaaatt attcatttcc gctcaccatt tattgtgatt tagcttagta acaaacaatt
    35401 gttaagaaaa ttgttactat ttgttaaatt aatttagtta atttggaaat aaaattatac
    35461 tagtatgaaa tccgtaaaca agattatcct gttttttgtt ttatgcatta gtagttttac
    35521 gcttttagca cagactaaaa ttaaaggtaa agttacagat ggtaaagagc caataattgg
    35581 agcaaacatc accattaaag gcacaaccga aggcactgtg agtgacctcg atggaaagtt
    35641 cgaactttct acaaaccaag caacaccact caccttagtt atttctatgg tcggtatggc
    35701 aacgtctgaa gtgaaatttg acggtagcca agaagaaatc agtgtaagca tgtctgaaga
    35761 gactagtctc ttaaatgatg ttgttgttgc tgcatctcgt gtggaagaga aaattctcga
    35821 atcgcctgtc accattgaaa aaatggatcc aaaagccatc aaaacagcag cagcagctga
    35881 ctattatgat gatttggcaa aattaaaagg cgtacaaaca attaatggtt caatgacact
    35941 cacttctgtc aacacgcgtg gttttggtgg tatctccaat actcgctttg tacaattgat
    36001 ggatggaatg gataatgcag caccattatt gaatttccca acaggtaata tagttggtat
    36061 cggcgaatta gacatcaata atgtagagct tgttcctggt gctgcttccg cattatacgg
    36121 tccaaatgct tttaatggta tcttattgat gaattcaaaa aatccattcg aacaacctgg
    36181 atttagtatg caagtaaaag gtggtttcgc acaagcaaac aacggctatg gtgttaaacc
    36241 tttgggttca gctgctttaa gaattgctca tgcattcaaa tctaaaaaaa caggtcaaga
    36301 ttttgccgca atcaaagcta acttcagtat tttccaaggt actgactggt atgcaaatga
    36361 ttatactaca gatagaagct taggcacaca agataatgta ggcaatccaa attttgacgg
    36421 acttaactta tatggagatg aagctagaat tttcgtgcct tatggtgctg ttcgtggaaa
    36481 tttaatcggt agtgcttcta atagtttaca aaattcacca ctcttccaag ctggcgttac
    36541 tgcagccgtt acagcacaag taaatgctca attgcctgct attacaacag ttgtaactgc
    36601 aactgtaaga caacaagcct acgacaatgc aattgctggc ggtgctgatg ttgcgactgc
    36661 aaatacaatg gctgatgcgt atatcgcttc tccagctggt caagctacta tacaagcaca
    36721 tgtacaagca caagtacaag cacttattga ttctaatgtt gctacgcagt tgccaataca
    36781 agcaaatgct cgtgctactt caaaagtgga ctctattagc tcattattat ctttagattt
    36841 aagacgttct ggtttgtcag aagatagatt attggataat cacaaagcac agtctattaa
    36901 aggcgacttt ggtgcttatt tcagaccgtt taaagataaa tcttatgaat tttcttataa
    36961 ctatagaata ggctttggta atagcgttta tcaaggtagt gaacgttatg ctttaagaaa
    37021 tttcttacaa caattccaca aagcagagat taaaagtaaa gactttttta tacgctctta
    37081 tatgtcacaa accaaagatg gtgattctta caacttgtct gcattaggag catattctaa
    37141 tgaagcagtt tcgccttctg gtagtcaatg ggtgcctaca tatttaagtt cttatgctgg
    37201 tccattaatc ggtgctgcac ttcaaacttc agcagcaaat gtattagcaa atgctgctgc
    37261 tttaacttca atggcacatg atacagcaag agcaagagca gacagacctt ggaataattt
    37321 aacagctgct gaacaacaag cagtgataga aagtgttcgt actggcttgt tccagagagg
    37381 tggcgctggt tttatcgata actctcgttt gttccacact gaagcaatgt tagatttaac
    37441 acgttggact ggtaaatgga ttgatattca aatcggtggt aaccacagat tatatagctt
    37501 atctactcaa ggtacagtat tcaatgaaga tccaaacgga acaggacaat acaaaagaat
    37561 aaaaatcaat gaatatggtg cattcttgca attgcaacgt aaattctata aagatatgtt
    37621 caagattcaa gcttctgtac gtttcgacaa aaaccaaaat tttaaattaa tcatctcgcc
    37681 tcgtgctgct tttgttgcta cattaggtaa agatagaaat cacaatattc gcgtatctta
    37741 ccaaacaggt ttcagaaatc ctgactcgca agcgcaatac atttatttcc caacaacaaa
    37801 tattttgtta ggtggtacaa aacaaaatgc agagcgttat ggtatatata atggcggtgc
    37861 ttatactatg gattcttata atgaatttgt ggcaacggct ttagctggcg cacctgatgt
    37921 tacgaaatta gttgtatctg attttgatta tgtaaaacca gaacaattac aagttgttga
    37981 attaggttac aaatcagtat tattcaaaaa attgatgatt gatgtaaacg gatatttcaa
    38041 tatctataaa gattttatta cacaaattac agttgtaagt atcgatagta cttaccacaa
    38101 aggtgtttta ttgccaggtg ttgcacaagt aattagtggt tctgcgcgta cagctacaac
    38161 ttggagacct tatgtaaaca ttcctggaaa aacattctct tatggtgcag gtattggtat
    38221 tggttatgca ttgcctaaaa atattatggc gcgtgtaagt tacaactaca tggattataa
    38281 aattaaaggt ggcaaacctt ctgttggtac tgctaatgac ttaggtttta actcttctaa
    38341 acatcaagtg ttcgttggtt tctctggcga tagagtttgg aaaggacttg gtttcgcaat
    38401 cgactacaga tggcaatcag caattgactg gagctctgac tttgctgacg gtactgtaaa
    38461 agcacgcggt atgttagatg caagtttaag ctacttcgta gagaaagcaa tgactacttt
    38521 taaaattggt gctactaata ttgctggtcc aacttacaga acaaatgttg gtggtccatt
    38581 tattggcaga acattcttcg cagccatcac atttgaccaa aacaaattat ggaaagaaca
    38641 ccataatgaa gtgactggtg ctaaagaatt ttaataattt ttcatactaa tagaaatgcc
    38701 tcgatttttc gaggcatttt ttttatacgt tctttgttat attcaaataa catgcgttta
    38761 agtaaatatt aattgatatt acttagataa tgatatttca ataactgctg acaaatccgt
    38821 atttttacaa ctgataatag catactaaac ttatatgcta ttcacttgtt aattattaaa
    38881 aataaaaaaa tgcaagaagt atttatcgtt tcgatggcac gcacgccaat aggtgcatta
    38941 agtagcactt tgtctggatt aaatgttata gaattaggaa aaacagtagt caaagcagcc
    39001 atggagcgcg ctggtgtaaa tggcgaccag atagatgaag tttatttagg aaatgtatta
    39061 caagctaatg tgggtcaagc acctgcacaa caagtgtcta tagctgcggg catcaacacg
    39121 aaaacaccat gtacaaccat caataaagta tgtgcttctg gtatgaaagc gattatgtta
    39181 ggagcgcaat caatcatgtt gggtgataat catattgtag ttgtaggcgg aatggaaagc
    39241 atgtcgaatg cgccgcatta tttaccttca ggtagaacag gcattcgcta tggcaatggc
    39301 gaaattttag atgctatctc tcgcgatggc ttacaagatc cgtacaaaaa atatatgatg
    39361 ggcaatgctg gtgaagtatg cgcaaaacat tattccttta cacgtgaaga ccaagatgcg
    39421 tatgcaatag catcatataa acgtgcacaa gatgcgtatg caaatggcta ttttaaagat
    39481 gaaataattc ctgttgtaat tgcttctcgc aaaggagata tcaccatcag tgaagacgaa
    39541 gaatacaaaa aatttgatga agcaaaagta agtgcattaa aaccagcttt cgaaaaagaa
    39601 ggaacaatta cagcaattaa cgcttctaaa attaatgatg gagcaagtgc aatggtatta
    39661 atgagtggcg ataaggtaaa agaattaggc cttacacctt tagccaaaat catctcattt
    39721 gctgatgctt cacaagagcc agagtggttt acaacaacac cgtcaaaagc aattcctaaa
    39781 gcattgcaaa aagcaaacct aactactgaa caagttgatt tttatgaaat caatgaagca
    39841 ttctctgtag tagcattagc caatatgaaa gaaatgaata tcgctcacga caaaataaat
    39901 gtatttggcg gtgcagtagc attaggtcat cctattggct cttctggttg ccgtatcgtg
    39961 gctacactta tttctgtttt gaaaaataaa aatggaaaaa tcggtgctgc aggcatctgt
    40021 aatggcggcg gtggcgcaag tgctatcgta attgaaaaag tataattatt acacataaat
    40081 gatattaaaa atgcaatagt atttctattg cattttttta tttcaatgta gagttgtgtg
    40141 gaaaatttgt gcacaactta gtattcggtc gtttttactt attattaggt atataaggta
    40201 tctgatttaa taataaattt gtagcatcaa aattatctat tatgaaaaat cttatgaaat
    40261 tatttacggt gctggtagta gttatgtttt ctacaagcac atttgcagaa gagttaggct
    40321 taaaagtagc taacatgaat ttatacgcgc caacagctaa aggcaaaagc tcgtcaggtg
    40381 gtggtaaagc cttcgaagga aaaggatcaa aacaaatctc catcggtgtt ggtctttcat
    40441 cttatatagg tgctttcgga aaaggtggct tttattctta cactggatca agaggtggct
    40501 attattgggg tcgcggtgca ttttggtatt ctggtacttt aagtgtacaa gcagaatttg
    40561 gtgtgcatga ttatgtaggc gttggtctag ttactggtat tggcggtaga gctgtcggtg
    40621 gcggtggagt actatatgta ccagttggca tcttagccaa tttccacttc tttcaattag
    40681 tagctgacaa aaaaggctta ggtattggtg atgcattaga cttgtatgtc ggtttaaact
    40741 tcggtacagg tttaggtgct agtttaccta agggttcagg aaataatgtt agcggtatta
    40801 tttttgctgg tccacaattt ggtgttaaat acttcttcaa tcctaagatt ggcgtgtatg
    40861 gcgagtttgg ctatggcaaa agtgtattcg aggcaggtgt ttctataaaa ttataattga
    40921 aactacaaag tatatcaagt ccgccatagt gcggactttt tttattgcat gaattaatag
    40981 tatttttgaa cgaaattatt aatatgcgtt taccacttta ttttatctgc tgtgttgtcc
    41041 tattgttttc agctgttgct gtatatgcgg aagaaaatat tagttctatt gataaagaaa
    41101 agaaagagaa gaaacaaaaa aataaagcag atgatggtgt agaaggtgca gctgcaaaag
    41161 gcgattttgt cattcagcct gctattaact ttggtttcca ctctggattt gtgaagcaat
    41221 acagtggctt tggttatggc tatgcttaca ctggacttat tccaggtgta acgctcaact
    41281 tagattatgc tgcgcatgat tatgcatcgg taggcttgta ttatggtgtt tccttccaaa
    41341 aatttaccgt atcaaaggtg tcattattac atcatacatt tggcgcacgc gtgcagtttc
    41401 attggtggca aatgttggca gacaggtcag gcaaagaatt attagcagat aaaattgatt
    41461 tcgatataca cgcacacatt ggtggatatt atttgacaga gaaaaatctg attagcgatc
    41521 aaaaactaaa acatcttggc gttaatgctg gcggtggaat tggattcaag tattactttg
    41581 taaaacactt cggcgtagcc ttagatgcag gctacgaaga agcgtcttgg ttaaaacttg
    41641 ggttagctat aaaaatttaa gaaaagtctt ctgctaatat tttttccata ttgcgttctg
    41701 ctaatgcagc aatcgtcaag gctggattgg cacagcaaga gaaaccaggc atagcagcac
    41761 cgtcaaggca gtataaattg ctatatcctt tgagacggcc tgttgtgtcg ctggctaaac
    41821 catacacagc accacctaat ggatgataag taaagtcatc gccatagcct ttgccattga
    41881 aataccagaa ttgctctaaa gttccaccat ttgcagcatt taatctgtca acaaaacttt
    41941 tcatcgcttg tcttgatatt tccatttgcg atttgtcgaa atataaatca gcattatcag
    42001 tagcaggatt gtatttgaaa tgacctctac ttggattttt tgtaatggct aatgccaata
    42061 attgtcttaa ttctaaacca ataggaaatg gtgcttgttc tgctaataat ggtgttatag
    42121 gattgtttaa atcaccatac gctgaaacag gtacagagcc ttgctcagcg ccagtatctt
    42181 cgcgtaagtt ggaacgcatg gccataatgt ttccattatt tccccaatgt ttacctactt
    42241 catcgcttaa attagccaag ccacctaagt gttttgattt taataaaata ttcatcgtgc
    42301 ccacaacacc agcattgatg aataaatatt tacaagtata aatttttacg gcaacagttt
    42361 cgccttgttc attgatttta tgaactgtga gctcataatt tttattgctt aattgtctaa
    42421 cagaatcaac cttgtgtaag gtttcaatag ttagatttcc tgttgcattt gcttgtggaa
    42481 t
//
LOCUS       DAOQQV010000007        42231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_250_length_42231_cov_8.813899, whole genome shotgun sequence.
ACCESSION   DAOQQV010000007 DAOQQV010000000
VERSION     DAOQQV010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 42231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42231
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_42231_cov_8.813899"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1471)
                     /locus_tag="PLL59_01540"
     CDS             complement(215..1471)
                     /locus_tag="PLL59_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HMY22512.1"
                     /translation="MSKQQKIELMAPAGSYEAMMAAIHAGCDSVYFGVEQFNMRARAT
                     MNFTLEDLDKIGNICKENNVRSYLTLNTIVYDHDLSAIKTVLEKAKSSGITAVIASDQ
                     AVIASARAIQLEVHISTQVNITNIETVRFYALFADTVVLSRELSLIQVKKICDQIEKE
                     NICGPSGRLIEVEIFVHGALCMAVSGKCYLSLHTANASANRGACVQNCRRSYTVIDNE
                     DGHELAIENEYIMSAQDLCTIDFLDQVIDSGVKVMKIEGRGKGADYVKTTVECYREAI
                     NAYYDNTYTKEKVANWMERLQRVYNRGFWSGYYLGQELGQWNDAAGSKATTRKIYLGK
                     GSNYFDKIQVAEFKLESFELSVGDSVLITGPTTGAIETTVEELHTANGAVQTAKKGEN
                     CAFKLTTPIRSSDKLYKIVNATEPAH"
     gene            complement(1619..4957)
                     /locus_tag="PLL59_01545"
     CDS             complement(1619..4957)
                     /locus_tag="PLL59_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMY22513.1"
                     /translation="MQEATPYQPKNKIRIVTAASLFDGHDAAINIMRRIIQASGAEVI
                     HLGHNRSVQEIVECAIEEDANGIAFTSYQGGHVEFLKYMYDLLREKGCGHIKIVGGGG
                     GTILPTEIDELHAYGIDKIYSPDDGRALGLQGMINDLLQICDFPSGKNVSFDASKLQA
                     KNAHQIAQLISCAENFPIEYEQLESQISNLKSDTPILGITGTGGAGKSSLTDELVRRF
                     LLDFPDKTLAIISVDPSRRKTGGALLGDRIRMNAINNPRVFMRSMATRQTNASISKNI
                     SDAIAILKAAAFDLIIIETSGIGQSGTEIVDIADTFLYVMTPEFGAASQLEKIDMLDF
                     ADVVAINKFDKRGAQDALRDVKKQYQRNRNLWQQKPDEMPVYGSIASQFNDPGTNELY
                     KAVIQKVIEKSNLDWKSSLNLRTGNAEKIYIIPPSRVRYLSDITTANRNYDKWVNKQA
                     EIANNLYAFDRLKNLLKQDEVESLEKQFTLDAECKEILDTWKEKKDNYRNEYYVFKVR
                     DKEIKIKTHTESLSHLPIPKVCLPKYEAYGDILKWNLQENVPGEFPFTAGVFPFKREG
                     EDPARMFAGEGGPERTNKRFHYVSAGLPAKRLSTAFDSVTLYGNNPDVRPDIYGKIGN
                     SGVSICTLDDAKKLYSGFDLCSPNTSVSMTINGPAPTICAFFMNAAIDQECEKYIVQH
                     KMEAEVNKKIDQIYAAKNAKRPVYTGDLPEGNNGLGLLLLGVTGDQVLDKEVYEKIKA
                     NALSQVRGTVQADILKEDQAQNTCIFSTEFSLKLMGDVQEYFVQHQVKNFYSVSISGY
                     HIAEAGANPISQLAFTLSNGFSFVEYYLSRGMHIDDFAPNLSFFFSNGIDPEYAVIGR
                     VARRIWAKAMKNIYNGNDRSQKLKYHIQTSGRSLHAQEIDFNDIRTTLQALYAIYDNC
                     NSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNENPLQGAFIIEELTDLVEE
                     AVYAEFDRITERGGVLGAMETMYQRSKIQEESMYYETKKHTGELPLIGVNTFLSSKGS
                     PTVLPQEVIRSTQEEKDKQISNLKALHTRNSDKANLHLQQLQQAALNNQNLFDVLMDT
                     VKYCSLGQITNALFEVGGQYRRSM"
     gene            5108..7756
                     /locus_tag="PLL59_01550"
     CDS             5108..7756
                     /locus_tag="PLL59_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HMY22514.1"
                     /translation="MRKIALGLLCFVFFLMASAIIIPYFFKDKIIAKAKEEISNQVNA
                     KVDFKDIDLSILKNIQNFPNIALGIDNLTIIGIPPFAGDTLLTIGKTNASLDLMSVIN
                     DEQYKIEAITLKNATINALVNKEGIANWNILKPSEEKKESKPFALALNKLKLEDININ
                     YDDLKGGTVLKIVNFNHEGKGDFAAEILDYESSTAIEKVSLAQGIVPYLKSVAINLDS
                     KVNIDQKQNKYSFQQSKLTLNRLALLLNGFVQIPDSTKTILDISFNAEKTDFKSLLSL
                     IPAIYSKDFTDIVSSGILKLDGFAKGTLEGNIYPAFALNLKVDNGKFRYPKLPTDVTD
                     VFIDAHVKNSGGSLDNTVVDVPDFRLKLANEPIVAKLKVSTPISDPNIDLEAKGKVNL
                     GDVQKYYPIKDVQKLSGIANVDLALKARQSDIINKRYQNINAAGNISANAIEYASKDV
                     PKPISVKQLVLNFSPQYVDIAQCNAVIGNSDFDIKGKMENVIAYVMSKDAVITGSANI
                     ISNKLDANEFLPDSTTAKKSNAQKAKEVVRLPKNIDFTGTASIGELLYDQLSIKNLLG
                     NIHLQDEQLNLNNLQGNLLGGSIIASGFYNTKTDIPTANLSYNIKDFDIQEVYKFVSS
                     AKQAAPIMKFVTGAFLSNMNMKMSLNPDLSPDLITLDGIAGFKMPLANVKGVPALQQI
                     VAQTKLKQLENLRIENLDVKATIANGRVLVAPFETKVNNMKMTIGGSQGLDQTMDYAV
                     AIDVPWKDLGQATSFAEGLLAKNPIKQLNGMVPEIVRINLKVGGTFSKPIISVGKPDG
                     TNGVGSMKDVVKEQVQQQAQQLKEEVKTQAIQTLDTIKTQVKEEAKNKLQEILSGQKN
                     PNDTTPKKSLQDNVKDQLKEKFKWPR"
     gene            complement(7897..8199)
                     /locus_tag="PLL59_01555"
     CDS             complement(7897..8199)
                     /locus_tag="PLL59_01555"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMY22515.1"
                     /translation="MVRIKWLKSAKDDLKEIYDYVAFDSKRYARLQVEKIRDKTEILR
                     TQIKAGKINEEINQESVREIIEGNYRIIYRIIAKDEVHILMVHHGARDLTRRIKGL"
     gene            complement(8187..8369)
                     /locus_tag="PLL59_01560"
     CDS             complement(8187..8369)
                     /locus_tag="PLL59_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22516.1"
                     /translation="MITKAKLQGYIQDFPDEISIDELIDRLVFIDKLENRIRESNSNE
                     TIEEIELKTEMEKWFE"
     gene            8893..9678
                     /locus_tag="PLL59_01565"
     CDS             8893..9678
                     /locus_tag="PLL59_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22517.1"
                     /translation="MKNIIIIFLVSIINIQNVFSEDSKHQVAYKKHELNIALHYAQHK
                     NGDHPLEQITIFSPDRLYIADYQKVGKKVGRGISMRAQYNYFFKRKIFFATSINMFFY
                     SPMTVHYDWTKNSNTYITRDIFHPILSKSDNKGKLNFTANQFYADFGLGFVPIIKNRF
                     SWRLGLGFCLGYHSFTDPIVSWTYDYIYLPEDDQTYLNFHGFRSNTLSGVMVGGYGQT
                     SFNVDIVPDKIAIGLDYKFTITNSWFKNGVFPQNVGVQFNVKL"
     gene            10031..10870
                     /locus_tag="PLL59_01570"
     CDS             10031..10870
                     /locus_tag="PLL59_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HMY22518.1"
                     /translation="MQIAELKDTATQVRRDIVRMVHQCKSGHPGGSLGCADFLTALYF
                     EIMKVDTNFKMDGVGEDLFFLSNGHISPLFYSVLARRGYFDVKELGTFRHINSRLQGH
                     PTTHEGLPGIRIASGSLGQGISVAIGAALTKKLNGDDKTVFVLTGDGELQEGQIWEAL
                     LFAAAKKVDNLIVTVDWNDAQIDGSTQHVCDLGDLKAKFTAFGWDVLEMKGNVMDEVV
                     FTLTEAQLRTKKGKPVAILMRTEMGFGVDYMMGTHKWHGSAPNDEQLAKAMEQLKETL
                     GDY"
     gene            10893..11162
                     /locus_tag="PLL59_01575"
     CDS             10893..11162
                     /locus_tag="PLL59_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HMY22519.1"
                     /translation="MAILSMFYGLIVSMYYFDIQQHNLPHIHVKYQEQEGIFNIITAE
                     LIAGSLPIPKTRLVQAWIEIHKDELMANWELAVSGENIFKIEPLK"
     gene            11168..11410
                     /locus_tag="PLL59_01580"
     CDS             11168..11410
                     /locus_tag="PLL59_01580"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HMY22520.1"
                     /translation="MNPRVINVQSKENYTLLLTFDNSEQKVFDVKPFLEKGIFRELKD
                     ENKFKQVKPFLGSIQWQGGQDFCADTLYENAIPLVR"
     gene            11577..11765
                     /locus_tag="PLL59_01585"
     CDS             11577..11765
                     /locus_tag="PLL59_01585"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HMY22521.1"
                     /translation="MKYHKQLIIRNKKVVNAYRLSKEQIKELNKRHKEYIEGKGKSYT
                     WEEVEEYFKSKNTSKNKS"
     gene            11787..12749
                     /locus_tag="PLL59_01590"
     CDS             11787..12749
                     /locus_tag="PLL59_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMY22522.1"
                     /translation="MSLQNITYTDKKDTRSGFGVGIYEAGKENPNVVALCADLIGSLK
                     LEKFIENFPERFVQTGIAEANMIGIAAGLTIGAKIPVATTFANFGTGRVYDQIRQSVA
                     YSDKNVKICCSHAGLTLGEDGATHQILEDIGLMKMLPHMTVVVPCDYNQTRLATKAII
                     ERHGPTYLRFGRPVWPIFTPAEDETFQIGKAQFLSQGSDVTIIACGHLVWHAVLAGQE
                     LEKRGISVELINMHTIKPLDVDAILQSVKKTGCVVTAEEHQMAGGLGESVASVLSQQY
                     LAPLEMVAVKDTFGESGTPDQLLEKYGLMPKDIIAAVEKVISRK"
     gene            12948..13559
                     /locus_tag="PLL59_01595"
     CDS             12948..13559
                     /locus_tag="PLL59_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22523.1"
                     /translation="MSLIFIYSKGNEIASIYFKGKIFLLNGDTLNTKIHLSYYKGNFV
                     KNLYLDNFGHYLDAKNKVRGLNANKINGFEVKIDGVVYTFVSKNKSKNDDKKIFYHLL
                     NNKGSTVRLYEFYNKAIMASALAVGVIVASELADVLYMIEYNNGADKEILYFPKRKNF
                     RYDKLAFILSDNKDLEKKIEDMTYKYDDMPLIIAEYNNWYSSR"
     gene            complement(13562..14161)
                     /locus_tag="PLL59_01600"
     CDS             complement(13562..14161)
                     /locus_tag="PLL59_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22524.1"
                     /translation="MNKKEAIQSILQSYHYFEQQSAAYKSWGKVEVVQIAMRLANNEI
                     IYTQKNKPLSAITEQDIITEKSDSIFTNIFAKRKSVQVIFITQQEFASQVKEEIPPIL
                     DDQAQLLGVSVRIAKGDNDILHSLNGRFASILLNGKSICLGTSIDDAYVAAQLLEKTS
                     KAFVEAKYLGGAKSINRIEAWLMQKFYQFKYSKEAKKNK"
     gene            complement(14183..14764)
                     /locus_tag="PLL59_01605"
     CDS             complement(14183..14764)
                     /locus_tag="PLL59_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HMY22525.1"
                     /translation="MNIEQIKEEVCNAGKRLLKEGLVARTWGNVSIKVNDTQMVITPS
                     GRPYDELTPNEMVLVDFHTLKFDGNIKPSSELKLHCEVYKTRPHIKAVIHTHQMYASI
                     VAAAQKDVMVLNEEHQKILGAKIIKAAPYALPNTKKITVETARAIEQSNAALMSNHGV
                     VCIGEDLEKTFEVARTLEHACALFIESKKKSAC"
     gene            complement(14761..16290)
                     /locus_tag="PLL59_01610"
     CDS             complement(14761..16290)
                     /locus_tag="PLL59_01610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HMY22526.1"
                     /translation="MNCFVAYDVGTSSVKSILVDASGNILASAIEEYPLQMPNPGWVE
                     QIPHDYWSAICKATKRLVSSANIDKQTIKGIAFTTQAMGVIPMSKEGEVLYPNITWVD
                     GRAEKQAQQIMRKLGGKKIFKSIIGVEITGKDVIPKIIWLKENKPEIFKKTYKILDVN
                     AYLKFMCTGKMVAEWSGACSYSFDLKKKDWDRLIFKFTGAGTKKLPDLVRSIDEVGKL
                     TTQAAAEMGLHENIGVYGGCDDTQSAALGTTAISEGEAHIYVGTSAWVGVSTAKDKGF
                     KNGIFCLQSADPTKNIVVGITESAGVNTEWLLNTFYKNERATMSESELFTYLENEIKA
                     VPAGAEYLVMTPWFLGERCPVSTTTTRSTIFNLSHLHTRAHIARAHFEGIAYNLKWSI
                     ENLEKDFGFTIKELKITGGGGSQSKLWMQTIADITDRKIITTSQPKNAGALGAAMCAM
                     VGSGFLSDFQDIKKLIKTVDTFVPNEAHKSIYNELFQTYKSIYTSLKNTYANANQNRF
                     Q"
     gene            complement(16397..17851)
                     /locus_tag="PLL59_01615"
     CDS             complement(16397..17851)
                     /locus_tag="PLL59_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMY22527.1"
                     /translation="MSQTGFSISEYHDIKPIYQKLDKLVSMPMQHIEPNALEKYLDYY
                     KTKCTQSKAIYEKAQQYIPGGVQHNLAFNHPFPLTFTKAEGAYLYDADGNKYIDFLQA
                     GGPTVLGSNYPLVREKAIALINECGPSTGLLHEYEYKLAELICQNIPSVERFRMLGSG
                     TEAVMGALRLARIKTGKSKVIKIGGAYHGWSDQMVYDLRIPGTRFFDAKGIPFMMHWH
                     TQAVPPNDIDALESKLKWNRFRGGTAAVILEPVGPESGTYPVYKEFAQQARQLCDKYG
                     TLLIFDEVVTAFRIGMSGAQGYFGVKPDLTIFGKVVAGGYPSAGGIGGKAEYMDGLAA
                     GLQGGKKVKVGGTMAANPLSSCAGYYTLLEIKNTNACEKAGKAGDRITKGLNELIDKY
                     GLPFVAYNQGSICHLETAGTLFVKIKLSKIKSVLAEINERKKLMEEYGAAYMAEGIVT
                     LAGSRLYCSMADTDDVIDDALNRFERVFKNVKKA"
     gene            17949..18524
                     /locus_tag="PLL59_01620"
     CDS             17949..18524
                     /locus_tag="PLL59_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY22528.1"
                     /translation="MSRHLTFTKFLEQFGDFSEEIHSAIESKLQRIELPKRHILLKKG
                     KVCNHLYFMEKGLARNYYEEDNKELTNDITVEGELLVSFSSFISRSPSLETIELLEDS
                     TLTAFHYDDLNWLYEKYPEMERFGRLVAEYNYNSLVLKSYELKFKSSTERYEELFNSK
                     IELIRRAPIGIIASYLGMSIETLSRIRSKIN"
     gene            18580..19113
                     /locus_tag="PLL59_01625"
     CDS             18580..19113
                     /locus_tag="PLL59_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMY22529.1"
                     /translation="MKFTDEEILALFKQSDTKEKAFRMLLSAYQERLYYHVRRYVHNH
                     EDANDILQNTCIKVWNAIDNFRADCALYSWLYRIAGNEAITFINKQKKRNEIDIEQSS
                     AQYRSASDAIGADAMTQKLQRAIDTLPDKQKQVFIMRYYDEMPYEKMAEISETSVGAL
                     KASYHHAVKKIEEILTA"
     gene            19176..19805
                     /locus_tag="PLL59_01630"
     CDS             19176..19805
                     /locus_tag="PLL59_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22530.1"
                     /translation="MSSQQDILNELKEIAPLLLKAKENERPLIIPANYFQDFADSCMS
                     EMKISTSILSAIKKDEIEVPETYFNTFSDTIMAKIKQEETHISNAQPIATKTNKNTFV
                     LTLFQRVALAASIIGAVFVVKEVNIPIAPAQDCADGIACLTREEIYQYMNANANAFDL
                     QQIEKAVAPSIEQQKTTQEIQDITEKDIDQYLEQNNNIIATEDLATDIF"
     gene            19824..20291
                     /locus_tag="PLL59_01635"
     CDS             19824..20291
                     /locus_tag="PLL59_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22531.1"
                     /translation="MKKILFLMAIVLAGILQLQAQDGNAKIEALKIAFITKRLNLNAD
                     EAQKFWPLYNQYEAEKKQVRQSTIGGVKEMKEDGDFTNAEAEQAITKYIEFKAKDLDI
                     TKKYVAEFRKIIPATKIAKLVTAEEHFKKMLTKQAQQGGPKSGNGQGGKPWKQ"
     gene            20467..21423
                     /gene="metF"
                     /locus_tag="PLL59_01640"
     CDS             20467..21423
                     /gene="metF"
                     /locus_tag="PLL59_01640"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HMY22532.1"
                     /translation="MKVTDILAENKEKTLVSFEILPPLKGKSIDSITKVLDPLMEFKP
                     PFIDVTYHREEFEYKQTAEGYFEKVAVRKRPGTVGICAAIKYRYNVEAVPHFLCGGFS
                     KEETENALIDLGFLGIHNILALRGDARKLEKKFVPEPDGHPFALDLVQQINDMNCGKY
                     LDHDLINPVKTDFCIGVAGYPEKHFEAANYEQDLQYLKQKIDAGGDYIVTQMFFDNKA
                     YFKFVEDCRKVGINVPIIPGIKPITKQGQLNSIPAAFFINLPEDLVREVRKCKDDTQV
                     KEVGIEWCIAQSKELVQFGVPCLHYYTMGDVKTISTICKELV"
     gene            21627..22097
                     /locus_tag="PLL59_01645"
     CDS             21627..22097
                     /locus_tag="PLL59_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMY22533.1"
                     /translation="METNMFMKRAIELSKMALQTGKGGPFGCVIVKDGKIVGEGHNQV
                     TSTNDPTAHAEVVAIRNACKNLNSFQLEGCEVYTSCEPCPMCLGAIYWARPSKVYFAN
                     TREDAKQIGFDDSFIYDEIPLSISQRKIPMLSMNRNQALLVFEEWKNKSDKTEY"
     gene            complement(22135..22752)
                     /locus_tag="PLL59_01650"
     CDS             complement(22135..22752)
                     /locus_tag="PLL59_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HMY22534.1"
                     /translation="MSFIYAIDLAGTFVFTISGALAASDKKHHHDIFSVFFTGFITAI
                     GGGTIRDVTLGAYPVVWINDMNYLLSIFAGVLFTLLFRRYIIRISRTLSIFDTIGMAI
                     YVVLGVQKSIVYDVNLLAAIVLGMVSGIFGGVIRDTLLNEIPLIFRKEMYATPCLLGS
                     LLFVILYVLQIDSNLNFMLCVLFIVLFRFFAVRYEWSLPRLNFKE"
     gene            complement(22787..23467)
                     /locus_tag="PLL59_01655"
     CDS             complement(22787..23467)
                     /locus_tag="PLL59_01655"
                     /inference="COORDINATES: protein motif:HMM:NF025660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HMY22535.1"
                     /translation="MRNCIVIFVVMLQLSAMAHAPEKAPKKNAKPKPTYEKPMPINTH
                     VWTVSAQRASCVGVGEMHCLLVKQANEKNFQLFYDNIEGFEYTEGYEYVIWVRKELKS
                     PPIPADASIYKYVLVKIVSKTSVKAQNENTLNIENASTTDTTTIVVHEKKAPCEGNNN
                     AMCLQIKKAGVSNFEVFFQNISGFSFKDGIRQTILVSMRKAENPMVKQVQPIYTLIKV
                     LKSEIIDK"
     gene            23557..24288
                     /locus_tag="PLL59_01660"
     CDS             23557..24288
                     /locus_tag="PLL59_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase"
                     /protein_id="HMY22536.1"
                     /translation="MKTLRIHHLQHVAFESLGMLQDWIDAHRFHQSATHLYKQETLPN
                     VDEFDVLIVLGGPMSVHDEQEFPWLKQEKTLIKQAIEHEKYVIGICLGAQLIANVLGA
                     RVYPNNEKEIGFFPVQKVDVSAEKQSEKILQLQALLHQQTVFHWHGETFDLPINTKHI
                     LQSAACLHQAFLLDDNVLGLQFHIEMNEAAIRTIIAHCKTELIDSNYIQSEALMLNEM
                     EKYIANNRTILFAVLDAILFSTPKL"
     gene            complement(24269..25054)
                     /locus_tag="PLL59_01665"
     CDS             complement(24269..25054)
                     /locus_tag="PLL59_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HMY22537.1"
                     /translation="MKKKTIFLIALVVFFPLNNLPTYASEKTSADVGSALAYLAKDNL
                     QKDALLEEAAVNSFLDCFSFVSDDAMKYALTGYAKLNNEGKINKRDIITIVDFSKPST
                     EERLFVINLRTKQVIAKSLCAHGRNSGEVWAEKFSNNAESYQSSLGFYICNETYDGKN
                     GFSLKLDGQEVGINDKARNRGVVIHGADYVSKQFIANNGKLGRSQGCPALPLEKNEKI
                     INLLKGGSCLFIYHPNKYYKKHSSILKNYNESCLAEVLTTLEY"
     gene            complement(25278..26186)
                     /locus_tag="PLL59_01670"
     CDS             complement(25278..26186)
                     /locus_tag="PLL59_01670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY22538.1"
                     /translation="MKKIGLITITYNSAHLIEPFLKCVLAQTHTNFILYIIDNISSDN
                     TLERIAKYKDERIVPIANTTNVGVAAGNNQGIKMAMNDNCDELLIINNDVEFENTLLE
                     KLTAQLIELQCSLIAPKMMYHPETHLIWWAGTKFRFIDGCMTNHIGIQEEDKGQYNTI
                     EKMDYAPTCCVLLKKEVISDIGIFDEKYFAYYDDTDFFYRIYKQQKHQLYYYPFVQFY
                     HKVGGLSNMKNGNAQKFKFNDFYIRLFTRNHVYYLKKQKNLIAYLNIIYFYFRMNLRF
                     LFSGKYHRNWKTFRLLQQSYFQGVKM"
     gene            complement(26188..27330)
                     /locus_tag="PLL59_01675"
     CDS             complement(26188..27330)
                     /locus_tag="PLL59_01675"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMY22539.1"
                     /translation="MKNILITGGAGFIGSNLSLHLLQKGYAITVLDNLHPQIHGDNPD
                     KTSPIYQSIKEKVRFIKGDVSNKSDWHSALQNQHIVVHFAAETGTGQSMYEISRYTHV
                     NVQGTALLLDYLANEKHHIEKVIIASSRAVYGEGKYFSETEQQYYYPKDRAEQDLQNG
                     IFELRNPNNADEFLKVVATDEESKIQAKSIYGLTKYHQEQALMICCQSLNIPCVAFRY
                     QNVYGPGQSLSNPYTGILSIFSTRILQHKDINIFEDGNESRDFVYIDDVVLATTAGIE
                     KEEANFQVFNVGTGTATSVLTVTQALMKNYGIEIPLAISGQYRKGDIRHNFADIQLLK
                     NILGFTPNIDFETGITKFCNWVKAQEIQQDKYEQSLAELKAKGLFK"
     gene            complement(27391..28326)
                     /locus_tag="PLL59_01680"
     CDS             complement(27391..28326)
                     /locus_tag="PLL59_01680"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY22540.1"
                     /translation="MQQTVQILLATYNGERFLHEQLDSLFNQTFQDFSVLVRDDQSTD
                     NTLHIIQEYQLKYADKIKIITDEKKNVGATQNFNLLLANTNADYIFFCDQDDIWLPHK
                     MEKSLALISTIEMDKNMPCLIYADMKSINDLGEVTAKSVWKQLHLSPKYFTLNRLLIQ
                     NIPHGCTMLINKAMRDMAFPIPETALLHDHWIALLAATCGQAIAIEEPLLLLRNHSEN
                     VTRKQTNFSDKMKRYSNNAISSNMYEYFIRIRVQQAKALQSRIGAKASEHVQLAIQNF
                     IALETTKGIRRKLIFLQNKFYRTTFWHTFKMIVRA"
     gene            complement(28326..29246)
                     /locus_tag="PLL59_01685"
     CDS             complement(28326..29246)
                     /locus_tag="PLL59_01685"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HMY22541.1"
                     /translation="MTLLISLDYSLFYVVKKFMFILVQMSQQPVIILGMHRSGTTMIT
                     KLLENLGLFVGKEKEINHEALFFWEINNWIFDIHTATPEKPHNLRYKNPACAQVIYES
                     LEYFIQSSKRKQYLGKHASAYQNIKDIDFPFGWKDPKNTFTIDFWKHIFPNPKIIHIY
                     RNPIDSVSSYIERDLVLKNKFEWNWKKKLKRQFLVSKNFHENFRLYNIEQGYELWQEY
                     VSTALSLQHTHENYISIKYEEFLSNPKEQLQQLCEYCGLQTTDEQIAKEIASIKTDRA
                     YAFLSNEDYTAYYQKIKQFPLMQTLGYHQL"
     gene            29311..30015
                     /locus_tag="PLL59_01690"
     CDS             29311..30015
                     /locus_tag="PLL59_01690"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMY22542.1"
                     /translation="MEKVLEILYEDNHIIAVNKRSGDIVQADETGDTPLSEIVKSYIK
                     EKYQKPGDVFLGVVHRIDRPVSGIVLFARTSKALTRLNELFKNKTIQKTYWCVVKQKP
                     KLNEATLQHYHIKDAKQRKAKLYDKEVAHSKKCVLHYKLLVSSDNYHLLAVQLETGRF
                     HQIRAQLAKIGSSIKGDVKYGFERPNENARSIHLHARKIEFEHPVKKEKIEIIAPLPD
                     DKIWKYFDEKMLHYQQ"
     gene            30077..30430
                     /locus_tag="PLL59_01695"
     CDS             30077..30430
                     /locus_tag="PLL59_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22543.1"
                     /translation="MKNSIFCIAIIAIFFTACKKEESATADVKGTWNLQLWDGVPASG
                     TLVFTATTLDFNCSTYSISEQTTYTNSGNTYVFTKTGGPSAVIAAGNNWHMDTLTANV
                     LRMTSHFGLIVRATK"
     gene            complement(30433..31263)
                     /locus_tag="PLL59_01700"
     CDS             complement(30433..31263)
                     /locus_tag="PLL59_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMY22544.1"
                     /translation="MKIIFHFCKVKEISSSANPLIKKIVHLQEKSRARKEENIFVIDG
                     WKETKMALENGFSIETILCQQSAYHTSWSEEFKNTIAPLSYNIESIIVTDDVFDKISY
                     RGNTSKVLAIAQQKSHQLSSIEKEKNPVFLILDGIEKPGNLGAILRIADATDITAIIC
                     CDTKTDIYNPNVIRSSVGCIFTQQIIICEKEAALQFLENNKVNIFTTSLKAAQNYLHC
                     DYKQASAFVFGTEATGVDAFWENHSRQNIIIPMRGQNDSLNVSNTVAIVLFEALRQRG
                     "
     gene            31274..31852
                     /locus_tag="PLL59_01705"
     CDS             31274..31852
                     /locus_tag="PLL59_01705"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HMY22545.1"
                     /translation="MKSTKEIIRCGWCVGKPLYEQYHDDEWGKPVHDDKKQFEFLVLE
                     SAQAGLSWYTILQRREAYRKAFANFDYKKVAKYDDKKVELLLQDASIIRNKAKITAAI
                     NNAKKFIAIQKEFGSFSAYIWNFVGGKPIVNKWQSLKEVPATTIESDALAKDMKKRGF
                     QFLGSTTLYAHMQATGLVNDHLLTCHAYKKCK"
     gene            complement(31853..32428)
                     /locus_tag="PLL59_01710"
     CDS             complement(31853..32428)
                     /locus_tag="PLL59_01710"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMY22546.1"
                     /translation="MTKLPKKIIETERLLLQPYHINLYKEVYVLIQKNKARLMHSFPN
                     MLKATQTMENTKDYVQQKSFDWNNNKAFAYLLIDKNSQALIGHFNIKDIDWKKKEAEL
                     AYFIDIDYEKKGLITEAMQLIIRLCFTYLPFEHIFARIVIDNLSSQKVVEKSGLKYEG
                     AFYNAYTTYDNMTVDTYRYGISKNDYIKMKA"
     gene            32491..33531
                     /locus_tag="PLL59_01715"
     CDS             32491..33531
                     /locus_tag="PLL59_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HMY22547.1"
                     /translation="MNLAYFSENSILPLVITPTEGESKKETLVQWIADNKASVESKLT
                     DSGAILFRGFQINTPQDFEDVAKAIDADLKDDYMGTSPRDKKSEYAFSASELPAHYPI
                     MQHCEMSFLPSAPRRLFFYCHVEPEYGGETPICDFRKVYQDLDPKIRAEFERKGVKHI
                     RNYSSPNEAARSGFQLKKWTDLFHTTDKKVIEEKCKENDIICEWKANDGLRMTNKNAG
                     IKEHPVTGEKVWFNHTQVFHISAAADEYYRIHKRQRRWETFKTSMFLELMTWYKKLTK
                     NPNEQSMHVTFGDDSEIPDDYVEHIQEVIWKHMVIQAWKKGDIICIDNFSTSHGRLPY
                     YGPREIMVCWTS"
     gene            33562..34458
                     /locus_tag="PLL59_01720"
     CDS             33562..34458
                     /locus_tag="PLL59_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22548.1"
                     /translation="MNSLENIQINGHGDSLLLLKIILAFILFSLAIDIKKEDFKVLFS
                     NPKSVIIGCISQIIILPLFTFILILILKPSASIAMGMLLVAACPCGNMSTYVSYLAKG
                     FIPLSISITAISTLLAGITTPFNFYFYASQYAPAKAVLHEIHISFLYLLSSIALLLLL
                     PLSAGITLKQYFPSFINRINKPVKLLALLFFIAFLVGAFITNSKIFIENLSQIFWFVF
                     LQNTLAFFIGYVFPKLFKLPASHCRSISIETGIHNSGLGLILVLAFFKGNGAMALIAA
                     WWGIWHIIAGMAIAWYWNKQLR"
     gene            complement(34450..35868)
                     /locus_tag="PLL59_01725"
     CDS             complement(34450..35868)
                     /locus_tag="PLL59_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22549.1"
                     /translation="MDKWFTHDDPVQFLFLNDLLLAPIFILITIILAKRYIKKKKNPA
                     YKKYFLPALYVKFVCAILMALVYQLYYEGGGDTHTYLTYSLRLREVLHENPIDYLKLL
                     FFTPDDVFLVEKYLGVGSAFYLNESSSLVIRITTLLSYPLFNTYLLISFVYTLFCFYG
                     CWKIFSLFSQLYPHLEKGFAVACLFLPSVCFWGTGILKDPICMGALGALTYHVYKLFF
                     EKTKIFRRLIFIFICLYLLKVIKVYILLSFLPAYSFWIAFRYKETIGSNLLKSMFGPV
                     IFVFATIIGVFILHKIATYSQRYSFESIVRTAKDTQNWLYYSSLKQGGSGYSLGNIDY
                     TLTGIIKVVPKAINVSIFRPYLWEAKKPILIPAAIEGLLFLFFTIRLLYKTGFIRFTR
                     LILTNPEVQFCLVFSLIFAFSVGFTSYNFGSLVRYKIPLLPFYFIALLILADKDKTAI
                     PELKKKPIKKKPELKLANLPLT"
     gene            36011..37300
                     /locus_tag="PLL59_01730"
     CDS             36011..37300
                     /locus_tag="PLL59_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22550.1"
                     /translation="MFAISSGWLSGVPSANVMLSNVLYGKLLSTLFSLNNSINWYTLI
                     LQLIFAICWVYLLCYFLFLKKNTLIISVFISAILLLGFIVAAITRPQFTSVALSCMVA
                     ALFTLYQSQHTTYRFAWFVFFIIVAILIRAESFFIFLVFALPIYFWNKSNRKQILRWI
                     AISILLFFVFYFVHYVFAIRPHEQNYMTLQTIDIIAAKPISIKEKILFQNNFSVQDIH
                     LIQSWLMADDAYNTQKMHALATALQSNRNCAQVFFEWKKFAQEERYLLVFYLFSLIAI
                     LFFAKKYRKFVLWNLILFLTVVLYLSITSRIPHRVTYPLLCYLVLFNIFHIIKSDFVI
                     KIKYAILSIFLLLSLYKFYCVLLLIPLHQSYQRQFEKTVSLIRQHDDLLFIGADAFPL
                     QYMNAWQAPSPSLLGNNLMLAGMQPHQITNYYYHNIS"
     gene            37336..37482
                     /locus_tag="PLL59_01735"
     CDS             37336..37482
                     /locus_tag="PLL59_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22551.1"
                     /translation="MIFLSASSSLQAAYIQVMKERYGIACHFEPIINKGYPVDSKRLV
                     FDAY"
     gene            complement(37476..38699)
                     /locus_tag="PLL59_01740"
     CDS             complement(37476..38699)
                     /locus_tag="PLL59_01740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY22552.1"
                     /translation="MPINSLYISYDGMTDPLGQSQVIPYLIGLTKNGYHISLISCEKA
                     ENFALYQSKIATLLSENNIQWHPISYTKKPPILSTLYDVFRIKKLATQLHHKHHFSII
                     HCRSYIAALVGLQLKKKLGLKFIFDMRGFWANERVDGNIWHLNNPIYKLVFNYFKKKE
                     IEFLENADYTISLTHNAKEEILSWKNIKNNPIKIEVIPCCVDLQLFNKTQIQQEKLYT
                     LQNELQIRQGDYVLLYLGSIGTWYMLDEMMEFFSVLKQKKEHAKFLFVTKEEHVRIKQ
                     TAAKYRVEDAIIIRPGIREEIPYLIALCQLSIFFILPSYSKKASSPTKQGELMAMGIP
                     IVCNTNVGDTDKVVIDFKSGILVNAFTQAEYINAIEQIDNKFDTAQIIKGAHDYFSLE
                     KGVEKYVSVYKNITQ"
     gene            complement(38699..40558)
                     /gene="asnB"
                     /locus_tag="PLL59_01745"
     CDS             complement(38699..40558)
                     /gene="asnB"
                     /locus_tag="PLL59_01745"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HMY22553.1"
                     /translation="MCGISGWLSLNKSLDKDAFKQATNKLAHRGPESFGFYFNENNTL
                     ALGHRRLKIIDLSDASAQPFYSACGRYVINYNGEIYNFKEIARDLQLTLKTSGDTEVI
                     IEAFAKIGTAAFTLLNGIFAFSIYDIHADKLYLCRDRIGIKPLYYYFDGIQFAFASEI
                     KALKAMPDLHMPINNAALAEFLHIGFLTEPDTAFQQVWKFPAGNFVEIDVKSLNNNTI
                     FPFTAYWNLHQQVLPNTHSNEAEVEKKLEELLIASIASQMISDVPLGTFLSGGIDSSL
                     ITALASKIKKDKIKTFSIGFQNSHFDETIYAEKVAKELGTDHFAYRMGEVDLEEILHE
                     IIDTYDEPFADSSAFPTMFVSKQAKKQVTVSLSGDGGDELFMGYGMHQWASRLDNPFV
                     RVFRKPFYMASQCMSFRYKRAAWLLNYDNHDHVASHIFSQEQYLFSEKDLKHYLVNPT
                     FNFDDINAIATSKRNLSSKERQSFWDLQYYLKDDLLVKVDRATMLYSLESRVPFLDND
                     VIDYTININEHLKYKNGEAKYILKKILYKYLPKELFNRPKKGFAMPLSHWLSANFKFL
                     IDKYLSPIVINRYDIVSNLHVAHLKNRFFAGEDYLYARIWSLVILHWWLEENA"
     gene            complement(40574..41521)
                     /locus_tag="PLL59_01750"
     CDS             complement(40574..41521)
                     /locus_tag="PLL59_01750"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMY22554.1"
                     /translation="MKSFLQYNNRILVTGGAGNVGSALVHRLIQDANNYVIIVDNLST
                     GTIEKLPTIGNNWKFIKCDANIYDDIMPIFLSYNIDYVFHYAATVGVQRTLDNPVSVL
                     HDIEGIKNILNLSKNTPVKRVFYSSSSEVYGEPVEFPQNEQTTPLNSKLPYAIVKNIG
                     EAFLKSYKQEFNLDYTIFRFFNTYGPNQSQDFVVAKFIKLALQNKDIPIYGDGSQSRT
                     LCYIDDNIEATINAFLSSNWENTTINIGNDNETTVLDIAKKIIEITNSKSNIIHLPAL
                     KEGDMTRRLPDISNMKTLLNNRPLVSLEDGIQKILEKWS"
     gene            complement(41533..>42231)
                     /locus_tag="PLL59_01755"
     CDS             complement(41533..>42231)
                     /locus_tag="PLL59_01755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY22555.1"
                     /translation="INEERAAARNRGTQLASGDYVTFFDSDDYLYPNHLSEAIKLIEK
                     HNNPEMIYLAYNVKDINHKVIKEQKIAKGTNFNKDLINGNYLSCNGVFLRRDVALQHP
                     FNETRALSASEDYDLWLRLAARYTIYYSDVITNTILNHDERSVLMIKKEPLIARINLL
                     IQTCTQDPMIQKVYAGQLDKFTAKCYLYVVLHLAMSSHKKTALSYFWKALTIHPLSIF
                     DIRVYGIIKCLIIY"
BASE COUNT        13634 a         7273 c         7298 g        14026 t
ORIGIN      
        1 atgacttgta taatatttac aggacaagct ttagcggcga gctgattgct ttctaattca
       61 tcttctcgca cttttacact ccaaaatcct tttttatctt ttgcgtcaag caatacactt
      121 ttgccgtctt tgcggctcat gcgccagcgc tgcggagcca actctacaca atagttgcaa
      181 ccaatgcatt ttgctctttg ctgtataata gtaatcaatg tgcaggctca gtggcgttta
      241 caattttata taatttatcg ctactgcgta ttggcgttgt caatttgaat gcacaatttt
      301 ctcctttttt tgcagtttgt acagcgccgt ttgctgtatg caactcttca actgttgttt
      361 ctatagcgcc agtagtgggt ccagtaatca atacgctatc gccaacactc agctcaaatg
      421 attccaattt aaattccgca acctgaattt tgtcgaaata attgctgcct tttcctaaat
      481 aaattttacg cgttgtcgct tttgagcctg ctgcatcatt ccattgtcct aactcttgac
      541 ctaaataata accactccaa aatccacgat tatatacacg ctgtaatcgt tccatccaat
      601 tagccacttt ttcttttgtg taagtgttgt cataatacgc atttatagct tcgcgataac
      661 actctactgt tgtctttaca tagtctgcac ctttgcctct gccttcgatt ttcatcactt
      721 ttacaccact atcaattact tggtctaaaa aatctatcgt acataaatct tgtgccgaca
      781 tgatatattc gttttcgatt gccagctcat ggccatcttc attgtcgatt acggtataac
      841 ttcttctgca attttgcaca caagcaccac gattcgcaga tgcatttgcc gtatgtaggc
      901 ttaaataaca ttttccagaa acagccatgc acaaggcacc atgcacaaat atttcgactt
      961 ctattaatct acctgatggt ccgcagatat tttctttttc gatttggtcg caaatctttt
     1021 ttacctgtat caaactcaac tcgcgagaaa gcacaaccgt gtcggcaaaa agtgcataaa
     1081 aacgcactgt ttcgatattg gtgatattga cttgtgtcga aatatgaacc tctaactgta
     1141 ttgcacgtgc cgatgcaata actgcctggt cgctagcaat tacagcagtg atgccactgg
     1201 atttagcttt ttccaatacc gtttttatcg ccgataaatc gtggtcatat acaatagtat
     1261 tcaaggtaag atagctgcga acattatttt ctttacagat atttccaatt ttatctaaat
     1321 cctctaaggt gaaattcatc gttgcacgtg ctcgcatgtt aaactgttcc acaccgaaat
     1381 ataccgaatc gcaaccggcg tgaatagcag ccatcattgc ttcatagctg cctgctggcg
     1441 ccatcaattc tattttttgc tgtttgctca tgatagtaca aagatacgac atcttctatt
     1501 tagccattgt aataaactgt tattattctt gtaattataa agcattgcaa tgcttttatg
     1561 taatatgttt aagtacttta tctgtcaaat taatttctaa aaataaagag ataaaatcct
     1621 acatacttct tctgtattgt ccgcctactt caaataaagc attagtaatt tgtccaagtg
     1681 agcaatattt gaccgtatcc atcagcacat caaataaatt ttgattgttt aaagcagctt
     1741 gttgtaattg ttgtaaatgt aagtttgcct tatccgaatt tcgagtgtgt aatgccttta
     1801 agttgctgat ttgtttgtct ttttcttctt gtgtactgcg aatcacttct tgtggcaaga
     1861 cggttggcga gccttttgat gacaaaaatg tattgacacc aattaatggc aattcaccag
     1921 tatgtttttt ggtttcatag tacatacttt cttcttgtat tttgcttcgc tgatacatgg
     1981 tttccatagc acctaagacg ccaccacgtt ctgtaattct atcaaattct gcatagacag
     2041 cttcttccac caagtcggtc aattcttcga taataaaagc accttgtaaa ggattttcat
     2101 tttttgccaa acctaattct ttattaataa ttaattggat ggccatagca cgacgcacac
     2161 tttcctctgt tggtgtggta atggcttcgt catacgcatt ggtgtgtaaa gaattgcaat
     2221 tatcataaat cgcatacaat gcttgtagtg ttgtgcgaat gtcgttaaag tcaatttcct
     2281 gtgcgtgcaa acttctgcca gatgtttgaa tatggtattt taatttctgg cttctgtcgt
     2341 ttccattata tatatttttc atggcttttg cccatattct acgcgccaca cgaccaatta
     2401 ctgcatattc tggatcgatg ccattgctaa agaaaaaaga aagattagga gcaaaatcat
     2461 caatgtgcat gcctcgcgac aaataatatt ctacgaatga aaaaccgttt gacaaagtga
     2521 atgctaattg cgagatagga ttggcgccag cttctgcaat atgatagcct gaaatagaaa
     2581 cagaataaaa attcttcact tgatgttgta caaaatattc ttgtacgtcg cccattaatt
     2641 ttaacgaaaa ttctgtagaa aaaatacaag tattctgcgc ttggtcttct ttcaaaatat
     2701 ctgcttgtac ggtgccacgc acttgcgata atgcattcgc ttttattttt tcgtacactt
     2761 ctttgtccaa tacttgatca cctgtaacac ctaagagtag taagcctaag ccattattgc
     2821 cttctggcaa atcaccagta tatactggtc ttttggcatt ttttgcagca taaatttgat
     2881 ctattttttt attgacttca gcttccattt tgtgttgaac gatgtacttt tcacattctt
     2941 ggtctatggc tgcattcata aaaaaagcac atatcgttgg tgctggacca ttaatcgtca
     3001 tggaaaccga agtgtttggc gaacataaat cgaaaccaga atacaatttt ttcgcatcat
     3061 ctaaggtgca aatagaaacg ccagagtttc caatttttcc ataaatatcg ggccgcacat
     3121 ctggattatt gccatataaa gtaacactat caaatgctgt tgacaatcgt ttggcgggca
     3181 atccagcaga tacataatga aagcgtttgt tggtgcgctc aggaccgcct tcgccagcaa
     3241 acatacgcgc agggtcttct ccttcacgct tgaacggaaa cacaccagct gtgaatggaa
     3301 attcgccggg aacattttct tgcaagttcc attttaaaat atcgccatac gcttcatatt
     3361 ttggcaaaca cactttagga atcggtaaat gcgacaagct ttccgtatgt gtttttatct
     3421 ttatctcttt atctcgaact ttaaacacat aatattcatt gcggtaattg tctttctttt
     3481 ctttccaagt atccagtatt tctttgcatt ctgcatcgag tgtaaattgc ttttctaaac
     3541 tttctacttc atcttgtttt agcagatttt ttaagcggtc gaaagcatac aaattattcg
     3601 ctatttctgc ttgcttattt acccatttgt cgtaattgcg atttgccgtt gtgatgtctg
     3661 ataaataacg aactctactc ggtggaatga tatatatttt ctctgcattt ccagtgcgta
     3721 aattcaggct agatttccag tctaaatttg atttttcaat tactttttgt attacagctt
     3781 tatatagttc attcgttcct gggtcgttaa actgtgaagc aatactgcca taaactggca
     3841 tttcatcagg cttttgctgc cataaatttc ggttgcgttg gtattgtttt ttcacatcgc
     3901 gcagtgcatc ttgtgcacca cgtttatcga atttattgat ggccacaaca tcggcaaaat
     3961 cgagcatatc tattttttct aattgcgatg ccgcaccaaa ttctggtgtc atgacgtata
     4021 agaaggtatc cgcaatatca acgatttccg taccactttg tccgatgcct gatgtttcaa
     4081 taataattaa atcaaaggct gctgctttta atatcgcaat agcatcgcta atatttttcg
     4141 atatcgacgc attggtttgt cgtgttgcca tcgagcgcat aaacacacgt ggattgttga
     4201 tagcgttcat cctgattctg tcgccgagca atgcacctcc tgtttttcga cggcttggat
     4261 caactgaaat aatggctaat gttttgtctg gaaaatccag taaaaaacga cgtactaatt
     4321 catctgttaa gctcgatttt ccagcgccgc cagtgcctgt aatacctaag ataggcgtgt
     4381 cagatttgag atttgagatt tgagattcca actgctcata ttcgatagga aaattttcgg
     4441 cacaagaaat caattgtgcg atttgatgcg cattttttgc ttgtaatttt gatgcgtcaa
     4501 aggatacatt ttttccgctt ggaaaatcac agatttgcaa taaatcattt atcatgcctt
     4561 gcaagcctaa agcacgtcca tcatcaggtg aataaatttt atcaatgccg taagcatgta
     4621 attcatctat ttctgttggc aaaatagtgc caccaccacc gcctacaatt ttgatatgtc
     4681 cgcaaccttt ttcgcgcaat aaatcgtaca tgtattttaa aaattctaca tgtccacctt
     4741 gatacgatgt gaatgcaatg ccgttagcat cttcttctat ggcacattct acaatttctt
     4801 gtacacttct attatgtcca agatgtatga cctcagcgcc acttgcttga ataattctgc
     4861 gcatgatgtt gatagcagca tcgtgtccat cgaacaagga agctgcagta acaattctga
     4921 ttttattttt cggttgatat ggtgtagcct cttgcataaa aatatattaa taacaaattt
     4981 acgaaatgtt gcttacagtt tgcaatatgt tgtaagccaa acatattagc attaaaaata
     5041 tctcctaaaa aatgtaaaaa taaattcaca gccatcatta ttatctattt ttgtggaaat
     5101 tattgctatg agaaaaattg ctttaggttt attgtgtttt gttttcttct taatggcttc
     5161 tgctataata ataccctact ttttcaaaga taaaattatt gctaaagcaa aagaagagat
     5221 aagcaatcaa gtcaatgcaa aagtggattt taaagatatt gacttgtcga tattaaaaaa
     5281 cattcaaaac ttcccaaata ttgcactcgg catcgacaat ctgacaataa taggaattcc
     5341 tccttttgca ggcgatacct tactaacaat tggcaaaacg aatgcatcgc ttgatttgat
     5401 gagcgttatc aatgacgaac aatataaaat agaagcaatt actttaaaaa atgcaacaat
     5461 aaacgcatta gtgaataaag aaggtatagc taattggaat attttaaaac cttcagaaga
     5521 aaaaaaagaa agcaagccat ttgctttggc tttaaataaa ttaaaattag aagatattaa
     5581 tatcaactat gatgatttaa aaggcggaac agtattgaaa atagtaaatt tcaatcacga
     5641 aggtaaaggt gattttgccg ctgaaatatt ggattacgaa tccagtacag caatagaaaa
     5701 agtgagcctt gcacaaggaa tagttcctta tttgaaatcg gttgccatca acttagacag
     5761 taaggtgaat atcgatcaaa aacaaaataa atattcattt cagcaaagta aacttacatt
     5821 aaatcgatta gcattattat tgaatggttt tgtgcaaatt ccagattcga caaaaaccat
     5881 cttggatatc tcatttaatg cggaaaaaac agatttcaaa tctttgttgt cgctgatacc
     5941 agctatttat tccaaagact ttacagacat cgtttcttcg ggcattttaa agctcgatgg
     6001 ttttgcgaaa ggaacgcttg aaggaaatat ttatccagca tttgcattaa acttaaaagt
     6061 agataacgga aaatttagat atccaaaatt gcctacagat gtgacagatg tatttattga
     6121 tgcacatgtt aaaaattctg gtggaagcct agataatact gttgttgatg tgcctgattt
     6181 tcgtttgaaa ttagcgaatg agcctattgt agcaaaatta aaagtaagca ctccaatttc
     6241 tgacccaaat atagatttag aggcaaaagg aaaagtaaat cttggcgatg tgcagaagta
     6301 ttatccaata aaagatgtac aaaaattaag tggcattgcc aatgtagatt tagccctaaa
     6361 agccagacag tctgatataa taaataagcg ctatcaaaat attaatgctg ctggaaacat
     6421 aagcgcgaat gcgattgagt atgcatctaa agatgtgcca aagcctatat ctgtaaaaca
     6481 attggtgctt aatttttcgc cacaatatgt tgatatagca caatgcaatg ctgtgattgg
     6541 caacagtgat ttcgacatca aaggaaaaat ggaaaatgta atagcttatg taatgtcaaa
     6601 agatgcagta attaccggct cagcaaatat tatatcgaat aaattagatg ctaacgagtt
     6661 tttaccagat tctacaacag caaaaaaatc gaatgcacaa aaagcaaaag aagtagttcg
     6721 tttgccgaag aacatagatt ttacaggcac tgcaagcatt ggcgaattgc tgtatgatca
     6781 attaagtatt aaaaatttgc ttggaaatat tcatttacaa gatgaacaat taaacctcaa
     6841 taatttgcaa ggaaatttac ttggcggttc aataattgca agtggttttt acaacaccaa
     6901 aacagatatt ccaacagcaa atcttagcta caatatcaaa gatttcgata ttcaagaagt
     6961 atataaattt gtaagctccg ccaagcaagc tgcaccaatt atgaaatttg ttacgggtgc
     7021 ttttttgtcc aatatgaaca tgaaaatgtc actcaatcct gacttatcgc ccgacttaat
     7081 aacacttgac ggtatagctg gatttaaaat gccattagca aacgttaaag gtgtgccagc
     7141 gttacagcaa attgtagcac aaacaaaatt gaaacaatta gaaaatctgc gtatagaaaa
     7201 cttggatgtg aaagcaacga ttgctaacgg cagagtttta gtagcgccat ttgaaaccaa
     7261 ggtcaacaac atgaaaatga ccattggtgg ctcacaaggc ttagatcaaa cgatggatta
     7321 tgcagtggct attgatgtgc catggaaaga ccttggacaa gctacatctt ttgccgaagg
     7381 cctattggca aaaaatccta taaaacagtt aaatggcatg gttccagaga ttgtaagaat
     7441 aaacttaaaa gtgggtggca cattttccaa accaatcatt agcgttggca aacctgatgg
     7501 aacaaatggt gttggctcta tgaaagatgt agtgaaagaa caagtgcaac aacaagccca
     7561 acaactaaaa gaagaagtga aaacacaagc aatacaaact ttagatacga ttaaaacaca
     7621 agtgaaagaa gaagcaaaaa ataaattgca agaaatactt tcaggacaaa aaaatccgaa
     7681 tgacaccacg ccgaaaaaaa gcttgcaaga caacgtaaaa gaccaattga aagaaaaatt
     7741 caaatggcct cgatgacaga tgtcatttcc ttctgtctgt cagcagacaa atgtaaaagt
     7801 taatcttatt gagttttaat agactatatg cgaactcttg tgcgaaagat aaacttctaa
     7861 ttattaaaat ggaatgagcc aataatttag atgctgttat agtcctttta ttctcctagt
     7921 taaatctctt gctccatgat gcaccattag tatatgcacc tcatctttgg caataattct
     7981 atagattatt cgataatttc cttcaattat ttctcttact gattcttgat taatttcttc
     8041 gtttattttg cctgctttaa tttgtgttct gagtatttct gttttgtctc ggattttttc
     8101 tacttgtaat cttgcatatc gtttagaatc aaaggcaaca taatcgtaga tttctttcag
     8161 gtcatctttt gcagacttca accattttat tcgaaccatt tttccatttc tgtttttagt
     8221 tctatctctt ctattgtttc gttgctattg gattctctaa ttctattctc cagtttatca
     8281 ataaaaacta acctgtctat aagttcatca atcgaaatct catctggaaa atcttgaata
     8341 tagccttgta actttgcttt ggtaatcatg gagtattttt atactgcaat ttaaataaaa
     8401 tcagtaaaag ataaaattaa attacctagg taattttttt acataattac agatattaat
     8461 acatctaaaa agtgtaaaac aaaccttagc catggtttgt gtcctcacga acacgttaca
     8521 aagcaattaa ggaatttgat tttttaaagg attttttttc agcagagcct ctcatagaga
     8581 actctgcgta attgttatga actaccgtag ttacacattg ccagtttcat gagttacagc
     8641 agggaaataa ataaccaaca aatctaatgt taattttgca attagtacaa aaagcatgca
     8701 ttttcatatc tatatttata attaatattt atataacata ccaaaaacgt ttaaacttct
     8761 aattttgtgg tctctaaaaa tgaataactc attaatctat tagccctggt ttgtgtccta
     8821 tcgaaccaca ttacaaagaa cagaccacaa tgatttaatc ttaacttata ataattatta
     8881 aagaacatac ttatgaaaaa tattattatt atatttcttg tttctatcat aaacatacaa
     8941 aacgttttct cagaagatag taaacaccaa gtggcttata aaaaacacga actaaatatc
     9001 gctttgcatt acgcacagca taaaaatggc gatcatccat tagagcagat tactattttt
     9061 tcaccagaca gattgtacat agcagactac cagaaggttg gtaaaaaagt aggacgtgga
     9121 atttctatga gagcacagta taattatttt tttaagcgaa aaatattctt tgctacatcc
     9181 ataaatatgt ttttctacag tccaatgaca gtgcattacg attggacaaa aaactcaaat
     9241 acttacatta cacgagatat tttccatcct atattatcta aaagtgataa taaaggtaaa
     9301 ttgaatttta cagcaaatca attttatgca gattttggtt taggctttgt accgattatc
     9361 aaaaatagat ttagttggag gttgggtttg ggattctgtt taggatatca ttcttttaca
     9421 gacccaatag tttcatggac ttatgattat atatatcttc ctgaagacga tcaaacttat
     9481 ttgaatttcc acgggtttag gtctaatact ttgagtggtg taatggtggg cggctacggt
     9541 caaacctctt ttaatgttga tatcgtccca gataaaatag ctataggttt agattataaa
     9601 tttacaatca caaatagctg gtttaaaaat ggtgtttttc ctcaaaatgt aggcgtacaa
     9661 tttaatgtga aactatagat tttttctcag cagagtctct cgtagaggac tctgtgtact
     9721 cagccatggt ttgtgtcctc acgcaccacg ttacaaagca attaaagaat ttgatttttt
     9781 aaagaattta tagaagcgtt ttaataatga tgcttcgtga ggacacaaaa catggcagct
     9841 tgcctcgata gtaattatta ttttatagca aacatagtat cttaagattt acagggtttt
     9901 tcatttctac tttagcaaaa tgatattcat cgttaacagc aacacaataa ttaatcattt
     9961 ttattttctt atttttgtaa agttgtaagt attagcttac agcggattgc tcatcactta
    10021 ttcttatcac atgcaaatag cagaactcaa agacaccgcc acacaagtac gtcgcgacat
    10081 cgttcgcatg gtacatcaat gcaaatcagg tcatccagga ggctccttag gttgcgccga
    10141 tttcttgaca gctttatatt tcgaaatcat gaaagtggac accaatttca aaatggacgg
    10201 tgttggcgaa gatttattct ttttgtcaaa cggacacatt tctcctttgt tttatagcgt
    10261 tttggcacgt agaggttatt ttgatgtgaa agaattaggc acatttagac acattaattc
    10321 ccgattacaa ggccatccaa ccacacatga aggcctgcca ggtattcgta ttgcgagcgg
    10381 ttctttagga caaggaattt ctgttgccat tggtgctgca ttgacaaaaa aattaaatgg
    10441 cgatgacaaa acggtgtttg tgctaactgg cgatggcgaa ttgcaagaag gacaaatttg
    10501 ggaagcattg ctatttgccg ctgcaaaaaa agtagataat ttaattgtta ccgtagattg
    10561 gaatgacgct caaatcgatg gttccacaca acatgtatgc gacttaggcg atttgaaagc
    10621 gaagtttacg gcatttggct gggacgtgtt ggaaatgaaa ggcaatgtaa tggacgaagt
    10681 agtttttacc cttacagaag cgcaattacg aacaaaaaaa ggcaaaccag tcgcgatatt
    10741 aatgcgtaca gaaatgggct ttggagtgga ctatatgatg ggcacacaca aatggcacgg
    10801 cagcgcacct aacgacgagc aattagcaaa agcaatggaa cagttaaaag aaactttggg
    10861 cgattattag aaatatgttt taattttgaa taatggcaat tctttcaatg ttttacggat
    10921 taattgtgtc tatgtattat tttgatatac agcaacacaa tttaccacat attcatgtaa
    10981 aatatcaaga acaggaaggt atttttaata taattaccgc agaattaatt gcaggcagtt
    11041 tgccaattcc aaaaacaaga ttagtacaag catggattga aattcataaa gatgaattga
    11101 tggcaaattg ggaattggct gttagcggag agaatatttt taagatagaa cctttaaaat
    11161 aattaccatg aatccaagag tgataaatgt tcagtcaaaa gaaaattata cattattgct
    11221 tacgtttgat aacagtgaac aaaaagtatt tgatgtaaaa ccatttttag agaaaggcat
    11281 ttttagagaa ctaaaagatg aaaataaatt taaacaagta aaacctttct taggcagtat
    11341 acaatggcaa ggaggacaag atttttgtgc agatacatta tatgaaaatg cgattccttt
    11401 ggtgcgataa aaagaaactt tgggtgatta ttgaagataa taagaataat aaggactgga
    11461 aaaattgtaa atcatatttt cacagtaaat gtagagttta attctccatc acaagcagct
    11521 acagttattt taggacgctc tgctaatggc aaaaaagagt ttaatataaa tagattatga
    11581 aataccacaa acaattaata attcgaaata aaaaagtggt caatgcctat agattatcta
    11641 aagaacaaat aaaagaatta aataaaagac acaaagaata tattgaagga aaaggtaaat
    11701 catatacttg ggaagaagtt gaggaatatt ttaaaagtaa gaatacatca aaaaataaat
    11761 cataattctg aaatcataaa tacaaaatga gtttacaaaa catcacttac acagacaaaa
    11821 aagatacacg ctcaggtttt ggcgttggta tctatgaagc aggaaaagaa aatcctaatg
    11881 ttgtagcact atgtgctgat ttaatcggct cgttgaaatt ggaaaaattt atagaaaact
    11941 ttccagaacg ttttgtgcaa acaggtatcg ctgaggccaa tatgattggt attgctgctg
    12001 gtttgaccat tggtgcaaaa attccagtag ccacaacatt tgcgaatttt ggcacaggtc
    12061 gtgtctatga tcaaatccgc caaagcgttg cctattctga taaaaatgta aaaatctgtt
    12121 gctcacatgc tggtcttact ttaggcgaag atggcgccac gcatcagata ttagaagata
    12181 ttggcttaat gaaaatgttg ccacacatga cagtagtagt gccttgcgat tataaccaaa
    12241 cacgcttagc aaccaaagcc attattgaaa gacatggacc aacgtatttg cgttttggtc
    12301 gtccagtgtg gcctattttt acgccagccg aagatgaaac tttccaaatc ggaaaagcac
    12361 aatttttatc acaaggtagc gatgtaacta tcatcgcttg cggacattta gtatggcatg
    12421 ccgttttagc tggacaagaa ttagaaaaac gcggtataag tgttgaactg atcaatatgc
    12481 acactattaa accgctagat gttgatgcta ttttgcaatc tgtaaagaaa actggttgtg
    12541 tggtgactgc agaagaacat caaatggcag gtggactagg cgaaagcgta gcatctgtgt
    12601 tgtcgcaaca atatttagcg ccattagaaa tggtagctgt aaaagatact ttcggtgaaa
    12661 gtggaacacc agaccaatta ttagaaaaat atggcttgat gccaaaagat attatcgcag
    12721 cagtagaaaa ggtgatctct agaaaataag atgttctcac agattacacg tatttctctg
    12781 aaacaaaatc tatggatatc tgtgatatct gtgagaaaaa atctctattc ttatttttac
    12841 acaatttcga tagcaattag acgcaacagt tttaaggagc aatgaaaaaa atataagtat
    12901 ttaagataaa taaaatggtt aacaaaaaat ttctacctat attttttttg tctttaatat
    12961 ttatctattc taaaggaaat gaaatagctt ctatttattt caaagggaaa atttttttat
    13021 taaatggcga tacattaaat actaaaattc atctaagcta ctataaaggt aactttgtaa
    13081 aaaatttata cttagataat tttggacatt atttagatgc taaaaataaa gtgcgaggtt
    13141 taaatgccaa taaaataaat ggttttgaag taaagataga tggtgttgtt tatacatttg
    13201 tatctaaaaa taaaagtaag aacgacgaca aaaaaatatt ttatcattta ttaaacaata
    13261 aaggtagcac agtacggtta tacgaatttt ataacaaggc catcatggct tctgctttag
    13321 ctgttggtgt aattgttgcg tcggaattgg ctgatgtact ttatatgatt gaatacaata
    13381 atggcgctga caaagaaata ctctattttc caaagcgtaa aaatttcagg tacgataaat
    13441 tagcctttat cttatcagac aataaagacc ttgaaaaaaa aatagaagac atgacataca
    13501 aatatgatga catgccatta attattgctg aatacaacaa ttggtattct tcaagataaa
    13561 actacttatt cttttttgct tccttagaat atttgaattg gtaaaatttc tgcatcaacc
    13621 atgcttctat gcggttgatg gatttggcac cacctaaata ttttgcttcg acaaatgctt
    13681 tcgatgtttt ctctaataat tgtgcggcaa cgtaagcatc atctatactt gtgcctaaac
    13741 aaatgctttt tccattcaat aaaatagaag caaatctacc gtttaatgaa tgcaaaatat
    13801 cattgtcacc tttggcaatg cgaacggaaa cacctaaaag ttgtgcttgg tcatctaaga
    13861 taggtgggat ttcttctttc acttgcgatg caaattcttg ttgcgtaata aaaattactt
    13921 gaacagattt tctttttgca aaaatgtttg tgaaaataga atctgatttt tctgtaataa
    13981 tatcttgttc cgtgatagcc gacagtggtt tgtttttttg tgtatagatg atttcattat
    14041 tagccaaacg cattgcaatt tgtactactt ctactttccc ccaactttta taagccgcac
    14101 tttgttgctc aaaatagtgg taagattgta agatagattg tatagcttct tttttgttca
    14161 tgtgtaaatt tttagatgca aattagcaag cacttttctt ctttgattcg ataaataatg
    14221 cgcacgcatg ctctaaagtg cgtgctactt caaatgtttt ttccaaatct tcgccaatgc
    14281 acacaacacc atgattagac atcagcgccg cattggactg ttctatcgct cgagcagttt
    14341 ctaccgtaat tttttttgta ttgggcaatg catatggcgc agcttttata atttttgcac
    14401 ctaatatttt ttgatgttcc tcatttaata ccataacatc tttttgcgca gctgctacta
    14461 ttgatgcata catctgatgc gtatgaataa cagccttgat gtgtggacgt gttttataca
    14521 cttcacaatg caatttcaac tcagaagaag gtttgatatt tccatcaaat tttaaagtat
    14581 gaaaatcaac caacaccatt tcattaggtg tcagctcgtc ataaggtctg ccacttggcg
    14641 taatcaccat ttgcgtgtca tttactttga tgctcacatt tccccaagtg cgcgctacta
    14701 aaccttcttt gagcaatcgt ttgcctgcat tgcaaacttc ttcttttatt tgttcgatgt
    14761 tcattgaaat ctattttgat tggcattggc atacgtattt ttcaaacttg tataaattga
    14821 tttataggtt tgaaaaagct cattataaat tgatttatgt gcttcattcg gcacaaaagt
    14881 atctacagtt tttattaatt ttttgatgtc ttgaaagtca gataaaaatc cgctgccgac
    14941 catggcacac atcgcagcgc ctaatgcacc tgcatttttt ggctgtgaag tggtaatgat
    15001 ttttcgatct gtaatatcgg caatcgtttg catccataat ttactttgcg atcctcctcc
    15061 accagtgatt tttagttctt taatggtaaa tccaaaatct ttttctaaat tttctatcga
    15121 ccatttaaga ttataagcaa tgccttcaaa atgggcacga gcaatatgtg cacgtgtgtg
    15181 taagtgtgat aaattgaaaa tagttgaacg tgttgtggtc gtacttaccg gacaacgttc
    15241 ccctaaaaac caaggtgtca tcactaagta ttcagcacct gctggaactg ctttgatttc
    15301 attttctaaa taagtaaaca attcagattc tgacattgtg gctctctcgt ttttgtaaaa
    15361 tgtatttaac agccattctg tgttaacacc agccgattct gtgataccta cgacgatatt
    15421 ttttgtagga tcagcacttt gcaagcagaa aattccattt ttaaaccctt tgtcttttgc
    15481 tgtagaaacg ccaacccaag cagatgtgcc tacatagata tgtgcttccc cttcgctgat
    15541 agccgtagtg cctaatgctg cactttgagt gtcatcgcaa ccaccataaa cacctatgtt
    15601 ttcatgtaaa cccatttcgg cagcagcttg tgttgtaagt tttccaactt catctattga
    15661 tcttactaaa tcaggaagct ttttagtgcc agcacccgta aatttaaaaa tcaaacgatc
    15721 ccaatctttc ttttttaaat caaatgagta ggaacatgcg ccagaccatt cagcaaccat
    15781 tttaccagta cacatgaatt ttaaatatgc attgacatct aaaattttat atgttttctt
    15841 gaaaatttca ggtttgtttt cttttagcca aataattttt ggaatgacat ctttccctgt
    15901 aatttcaaca ccaattatag atttgaatat ttttttacca cccaattttc gcatgatttg
    15961 ctgtgcttgt ttttctgcac gaccatcaac ccatgtgata ttaggataaa ggacttcacc
    16021 ttctttgctc ataggaataa cacccatggc ttgtgttgta aaggcaattc cttttattgt
    16081 ttgtttgtcg atatttgctg aagatacgag tcgcttagta gccttacaga tagccgacca
    16141 ataatcatgt ggaatttgtt ccacccaacc tggattgggc atttgcaatg gatattcttc
    16201 aatggctgaa gccaaaatat ttccgcttgc atctactaaa atagatttta cactacttgt
    16261 gccaacatca tatgctacaa aacagttcat ttttttcttc ttagtgatgc aatcaacacg
    16321 tcattcagtg ctatataaag acagttacaa tttagtagat gattgaaagt aatatgctat
    16381 tcagctacaa atattattac gcctttttta cgtttttgaa aaccctctca aatcggttta
    16441 aggcatcatc aattacatca tcagtgtcgg ccatgctgca atacaagcga ctaccagcca
    16501 aggtaacgat accttcagcc atatacgcag cgccatattc ttccatcaat tttttgcgtt
    16561 cattgatttc tgctaatact gattttattt tcgacagttt gatttttaca aataatgttc
    16621 cagcggtttc taaatggcaa atagaacctt gattatatgc cacgaatggt aaaccatatt
    16681 tgtcaataag ctcatttaat ccttttgtaa ttctatcacc agcttttcct gctttctcgc
    16741 aagcgttggt gtttttgatt tctaataatg tgtaatatcc tgcgcagcta ctcaaaggat
    16801 ttgcagccat cgtgccacct actttcactt ttttgccacc ttgcaagcct gcggcaagtc
    16861 cgtccatata ttctgctttt ccgccaatgc caccagcaga aggataaccg ccagctacca
    16921 cttttccaaa tatagttaag tcgggtttta caccaaaata gccttgggcg ccactcattc
    16981 cgatgcggaa tgcggtgacc acttcgtcaa atattaataa tgtgccatat ttgtcgcaca
    17041 attgacgagc ttgttgtgca aattctttat agactggata agtaccgctt tcaggaccta
    17101 ctggttctaa aataacggca gcagtaccgc cacggaatcg attccatttt agtttgcttt
    17161 ctaaagcatc aatatcattt ggtggaacag cttgtgtgtg ccaatgcatc atgaaaggaa
    17221 tacctttcgc atcgaagaaa cgcgtgcctg gaatacgtaa gtcatatacc atttgatcgc
    17281 tccagccgtg ataggcacca ccgattttta ttactttaga tttgccagtt ttaatacgcg
    17341 ccaatcgcag tgcacccata actgcttctg tgccacttcc taacatacgg aatcgttcta
    17401 cagaaggaat attttggcaa attagttccg ctaatttata ttcatactcg tgtaataaac
    17461 ctgtagaagg gccacattca ttgattaaag caattgcttt ctcgcgtact aatggataat
    17521 tgctacccaa caccgttggg ccgccagcct gtaaaaaatc gatatattta ttgccatcgg
    17581 catcatacaa ataagcacct tccgccttgg taaatgttaa tggaaatgga tgattgaatg
    17641 ctaaattatg ctgcacacca cctggaatat attgttgtgc tttttcatag atggctttag
    17701 attgggtaca ttttgtttta taataatcta aatatttttc caatgcattg ggttcgatat
    17761 gttgcattgg cattgacacg agtttatcta gcttctgata tataggtttt atgtcgtggt
    17821 actcgcttat tgaaaatcct gtttgactca ttttataatt ttctttatct ttgtatttga
    17881 tattgccgta caaagttgcg gctaataaga tattgatttt ttgataataa tcaaaatata
    17941 agatggaaat gtcgagacat ctaacattta caaagtttct ggagcaattt ggcgactttt
    18001 cggaagaaat acatagtgct atcgaaagca aattacaaag aattgagttg ccgaaaaggc
    18061 atattttact aaagaaagga aaggtttgta atcatcttta ttttatggaa aaaggcttgg
    18121 cgcgtaatta ttatgaagaa gataataaag agctaaccaa tgatattacc gtggaaggtg
    18181 aattattggt ttcattttca agctttattt ccagaagtcc ttccttagaa accattgagc
    18241 ttttagaaga tagtacttta acggcatttc attatgatga cttgaattgg ttatatgaaa
    18301 aatatcctga aatggagcga tttggtaggt tggtggcaga atataactac aattctttgg
    18361 ttctaaaaag ttacgaattg aagtttaaaa gctcgacaga acgctatgaa gagttattca
    18421 attcgaaaat agagttgata agacgtgctc caattggcat tattgcttcc tatcttggta
    18481 tgagtataga aacattaagc agaataagaa gtaagataaa ttaaaccttt ttgtttttaa
    18541 tgagtctaac atactattga tatgtaacaa tagcattgaa tgaaatttac tgacgaggaa
    18601 attttagcat tattcaaaca gtctgatacc aaagaaaaag catttcgcat gttgctgagt
    18661 gcttatcagg agcgcttgta ttatcacgtt cgacgatatg ttcacaatca cgaagatgca
    18721 aatgatatct tgcaaaatac ttgtataaaa gtatggaatg caatcgataa ttttagagct
    18781 gactgtgcat tatattcttg gttatatcgc attgctggca acgaggccat cacatttatc
    18841 aataaacaga aaaaaagaaa tgaaattgat atagagcagt catcagcgca atataggagt
    18901 gcaagtgatg ccataggtgc agatgcgatg acacagaaat tacagcgtgc gattgataca
    18961 ttaccagata aacaaaaaca agtatttatt atgcgatatt atgatgaaat gccttatgaa
    19021 aagatggcag aaatttcaga aacctctgtg ggtgcattaa aggcgtctta tcatcatgca
    19081 gtaaaaaaaa ttgaagagat attaaccgct tagcatcaac acataaaaat taaagtatga
    19141 ttaaaccttt atgtaaataa atagtctaac taaaaatgag tagccaacaa gatatattaa
    19201 acgaattgaa agaaattgct ccattacttt taaaggcaaa agaaaatgag cgtcctttaa
    19261 taattcctgc gaattacttt caagattttg cagatagttg tatgtctgaa atgaaaatat
    19321 caacaagtat tttgtcggct attaaaaaag atgaaataga agtgcctgaa acttatttta
    19381 atacgttttc ggacacgatt atggctaaaa taaaacaaga agaaacgcat atttccaatg
    19441 ctcagccaat tgcgacaaaa acaaataaaa atacttttgt gttgacacta ttccagcgag
    19501 ttgcattagc tgctagcatt attggcgctg tatttgttgt taaagaggta aatattccta
    19561 tcgcacctgc gcaagactgt gccgatggca ttgcctgctt gacaagagaa gagatttatc
    19621 aatatatgaa tgccaatgcc aatgcttttg atttgcaaca aattgagaaa gcggtagcgc
    19681 catcaattga acagcaaaaa acgactcaag aaatacaaga tattacggaa aaagatatag
    19741 atcaatattt agaacaaaac aacaacatca tagcaacaga agaccttgct acagatattt
    19801 tttaaaaaat taaaacaagc aaaatgaaaa aaatactctt tttaatggct attgttttag
    19861 caggaatttt acaactacaa gcacaagatg gaaatgcaaa aatcgaagca ctgaaaattg
    19921 catttattac caagcgctta aatttgaatg ccgacgaagc tcaaaaattt tggcctttgt
    19981 ataatcaata tgaagcagag aaaaaacaag tgcgccaaag cacaatcggt ggtgtaaaag
    20041 aaatgaaaga agatggagat ttcacaaatg cagaggccga acaagccatc accaaataca
    20101 tcgaatttaa agctaaagat ttagacatta ccaaaaaata tgttgcagaa tttagaaaaa
    20161 taattccggc taccaaaatt gctaaattag taactgccga agagcacttt aaaaaaatgc
    20221 tgactaaaca agcacaacaa ggcggaccaa aatctggcaa tgggcaagga ggaaagccat
    20281 ggaaacaata atgattttta tgtaatttac aggagccgaa ccaaaagttc ggcttttcta
    20341 tgttttattt tacctttcga atccattcca ttgacattgc gcaatcacta ttacatttag
    20401 taattgtact ttttatgata ttactattct atttactatt ttcgttgtcc atttgaaaaa
    20461 gtcgaaatga aagtaactga tatactagca gaaaacaaag agaaaacctt agtgtcattt
    20521 gaaattctgc cacctttaaa aggaaaaagc attgattcta ttacgaaagt tttagatcct
    20581 ttgatggagt ttaagccacc atttatcgat gttacctatc atcgcgagga atttgaatac
    20641 aaacaaacag cagaaggcta ttttgaaaaa gtggccgttc gcaaaaggcc tggcacagta
    20701 ggcatttgcg cagctattaa atacagatat aatgtagaag cagtgccgca ttttttatgt
    20761 ggaggcttta gcaaagagga aacagaaaat gcacttatag atttaggatt tttaggcatt
    20821 cacaatatct tagcacttcg tggcgatgca cgtaagttgg agaaaaaatt tgtaccagag
    20881 ccagatggac atccatttgc attggatttg gtgcaacaaa tcaacgacat gaattgtggc
    20941 aaatatttag accacgattt aatcaatcct gtaaaaacag atttctgcat cggcgttgct
    21001 ggctatcctg aaaaacattt tgaagcagcc aattatgaac aagacctgca atatctgaaa
    21061 caaaaaatag atgcaggtgg cgattacatt gtaacgcaaa tgttttttga taataaagcg
    21121 tatttcaaat ttgttgaaga ctgtagaaaa gtcggcatca atgtgcctat tattccaggc
    21181 attaaaccga ttacaaaaca aggccaatta aacagtattc cagctgcttt tttcatcaat
    21241 ttgcctgaag atttggttcg tgaggtgcgc aaatgcaaag acgatacaca agtgaaagaa
    21301 gttggcattg agtggtgcat tgcgcaaagt aaagaattag tgcaatttgg tgttccgtgt
    21361 ttgcactatt atacaatggg cgatgtgaag acgatttcta ctatttgtaa agaattggtg
    21421 taaattaaat taggaatggg caatccattc aaccggtata aaatagaaaa gcacatctta
    21481 atcgatgtgc tttcattttc atagtttttg ttttattttg tttttgtaat gtaaatatag
    21541 tagcaatctg attttctgtg aatataaatt agcaaacggt cgtttttaat gtgtattcgg
    21601 tagaatttag cttaaattta aaaaagatgg agacaaatat gtttatgaaa cgcgcaatag
    21661 aactgtctaa aatggcactt caaactggca aaggtggccc ttttggctgt gttatagtga
    21721 aagatggaaa aattgtagga gaaggtcata accaagttac gtccaccaat gacccaactg
    21781 cacatgcgga agttgtggcc attagaaatg cttgtaaaaa cctgaattca tttcagttag
    21841 aaggttgtga agtgtatacc tcctgcgagc cttgcccgat gtgcttgggc gctatttatt
    21901 gggcgcgccc ttcaaaagta tatttcgcca acacacgtga agatgccaag caaataggtt
    21961 tcgacgattc ttttatttat gatgaaattc ctttatcaat ttcgcaaaga aaaattccaa
    22021 tgctcagtat gaatcgcaat caagcacttt tggtttttga agaatggaaa aataaaagcg
    22081 ataaaacaga atactaataa ctacctgtgt tgcttgttta tgtattcatt tttcttactc
    22141 tttaaaattt aatcttggca agctccattc atagcgcacg gcgaaaaatc taaataggac
    22201 aataaacagc acgcacaaca taaaattgag attgctatca atttgcaaca cgtataaaat
    22261 aacaaataat aaggagccta aaagacaagg cgtggcatac atttcttttc tgaaaattaa
    22321 aggaatttcg ttgagcaaag tgtcgcgaat aacaccaccg aaaataccag aaaccattcc
    22381 taacacaatt gctgccaaca aatttacatc atacacaata cttttttgta cacctaatac
    22441 aacgtaaatg gccataccaa tcgtatcgaa aatactgagt gttcgcgata tgcgaatgat
    22501 atatcttctg aataaaagtg taaacaaaac acctgcaaaa atggataata aataattcat
    22561 gtcattaatc caaacaactg gataagcgcc gagcgtcaca tctctaatag tgccgccacc
    22621 aattgcagta atgaatccag taaaaaatac agagaaaata tcgtgatggt gttttttatc
    22681 ggacgctgct aaagcaccag aaatagtaaa tacaaaagtt cctgctaaat cgatggcata
    22741 tataaacgac atgatttaaa gataaggaat cctactaaat attaaattat ttgtctataa
    22801 tttcgctttt taaaactttg ataagcgtat aaattggctg tacttgtttt accatcggat
    22861 tttctgcttt gcgcatactg accaagatag tttgccgtat gccatctttg aaactgaatc
    22921 cacttatgtt ttggaaaaaa acttcaaaat tgcttacgcc agcctttttt atttgcaaac
    22981 acattgcatt gttattgcct tcgcaaggtg cttttttttc atgtacgaca attgttgtcg
    23041 tatctgttgt agatgcattc tcaatgttta aagtattttc gttttgagct ttaactgatg
    23101 tttttgagac tattttcacc aaaacatatt tataaataga agcatctgct ggtattggcg
    23161 gtgatttcaa ttctttacgc acccaaatca catactcata accttctgta tattcaaagc
    23221 cttcgatatt gtcatagaat aattggaaat ttttttcatt tgcttgtttt actagtaagc
    23281 aatgcatttc gccaacacca acacagcttg cacgttgtgc acttactgtc cagacatgcg
    23341 tatttattgg cataggcttt tcgtatgtcg gttttggttt tgcatttttc ttaggtgctt
    23401 tttcaggagc atgcgccatg gcagacagtt gcaacatgac aacgaaaatg acaatacaat
    23461 ttctcatttc tacattattt atttaaacat aaaatacgaa gtaaatatct atccaatttc
    23521 tcgactatct ttgttaatat atattaaaat caaagcatga aaacattgcg cattcaccat
    23581 ttgcaacatg tcgcattcga atctttagga atgctacaag attggataga tgctcatcgt
    23641 tttcatcagt cggctactca tttatataaa caagaaacat tgccaaatgt tgacgaattt
    23701 gatgtgttga ttgtattggg tggaccgatg agcgtgcatg atgaacagga atttccttgg
    23761 ctgaaacagg aaaaaacact gataaaacaa gcgatagagc acgaaaaata tgttattggc
    23821 atttgcttag gtgcacaatt aattgccaat gttttaggag ctcgtgtgta tcctaataat
    23881 gagaaagaaa taggattttt tccagtacaa aaagttgatg taagcgcaga aaaacaatct
    23941 gaaaaaatac ttcaattaca agcactatta catcaacaaa cggtttttca ttggcacggc
    24001 gaaacttttg atttgccaat caacacgaag catatattgc agtcagcagc atgtttacat
    24061 caagcatttt tgttggatga taatgtgtta ggtttacaat ttcacatcga gatgaacgaa
    24121 gctgcgatac gcacaattat tgctcattgt aagactgaat taattgattc gaattatata
    24181 caatcagaag ccttgatgct aaatgaaatg gaaaaatata tcgcaaataa tagaacgatt
    24241 ttgtttgctg tattagatgc aattttgttt agtactccaa agttgtgagc acttctgcca
    24301 gacaagattc gttgtagttt tttaagatgc ttgaatgttt tttgtagtat ttatttggat
    24361 gataaataaa caaacaagaa ccacctttta ataggtttat tattttctcg tttttttcta
    24421 atggcaatgc tgggcagcct tggcttctgc ccaattttcc gttgttggca ataaattgtt
    24481 tgctaacata atcagcacca tgaatcacaa cgccgcgatt gcgtgctttg tcgttaatgc
    24541 caacttcttg accgtctaat tttagggaaa atccattttt gccatcataa gtttcattgc
    24601 aaatatagaa acctaaacta ctttgatatg attcagcatt attagagaat ttttctgccc
    24661 aaacttcgcc actatttctg ccatgtgcac ataggctttt ggcgatgact tgttttgtgc
    24721 gcaagttgat gacaaacaaa cgctcttcag tagaaggttt gctgaaatct actatggtga
    24781 taatatctct tttgttgatt tttccttcat tgttcagttt tgcatatcct gtaagtgcat
    24841 atttcatcgc atcatctgac acaaatgaaa aacaatctaa aaaactgttc accgcagctt
    24901 cttctaaaag cgcatctttt tgtaaattat cttttgccaa ataagccaaa gcactaccaa
    24961 catctgctga tgttttttcg cttgcataag taggcaagtt atttaatgga aaaaaaacca
    25021 ctaaagctat caagaagata gttttctttt tcatacaaaa tattcttaac tatttacgtg
    25081 ttgtagaagg aagcattacg gctgcttttc tatatataaa aatacatcgt tttgcgcttt
    25141 gtacttactt ttggttacat gaaaatgctt aaaaacttgt taaatgctat ctttttagca
    25201 attttttaag agcaggcttg atatatgtca agcggtttat gttttaaaaa aagtaatgat
    25261 gatggcaata agtgcaatca catttttacg ccttggaaat aagattgttg taataagcga
    25321 aatgttttcc aattgcgatg atattttcca ctaaataaaa atcgaagatt cattcggaaa
    25381 tagaaataaa taatattgag gtaagcaatt agattttttt gttttttcaa ataatacaca
    25441 tgattgcgcg taaacaatcg aatataaaag tcattaaact taaatttttg agcattgcca
    25501 tttttcatgt tagaaagccc gccgacttta tgataaaact gcacgaaagg atagtaataa
    25561 agttgatgtt tttgctgttt ataaatgcga taaaaaaaat cggtatcatc gtaataagca
    25621 aaatatttct catcaaaaat gccaatgtca ctgatgactt cttttttcaa aagtacacag
    25681 cacgttggcg cataatccat tttttcaatt gtattgtatt ggcctttgtc ttcttcttga
    25741 atgccaatat gattggtcat acagccatca ataaaacgaa atttagtgcc agcccaccag
    25801 attaagtgag tttctggatg atacatcatt tttggtgcta tcaaactgca ttgtagctca
    25861 attaattgtg ctgttaactt ttcgagcaat gtgttctcaa attcgacatc attgttaatg
    25921 ataagtagtt catcacaatt atcattcata gccattttta taccttgatt attgccagca
    25981 gccacgccaa catttgtagt attggcgata ggcactattc tttcatcttt atattttgca
    26041 atgcgttcca acgtattgtc agatgaaata ttatcgatga tgtataaaat aaaatttgtg
    26101 tgcgtttgtg ccagcacgca tttcaaaaaa ggctcgataa ggtgtgcgct gttgtaggta
    26161 atcgtaatta agcctatttt tttcatatta cttaaataag ccttttgctt ttagctctgc
    26221 taaagattgt tcgtatttat cttgctgtat ttcctgtgct ttcacccaat tacaaaattt
    26281 tgtaatacca gtttcaaagt cgatgtttgg cgtaaaacca agtatatttt ttaaaagctg
    26341 aatgtctgca aagttatgtc tgatatcacc ttttctatat tgacccgaaa ttgctaatgg
    26401 aatttctatg ccataatttt tcatcaatgc ttgtgttaca gttaaaacgg aagtagcggt
    26461 gccagtgcca acattaaaaa cttggaagtt tgcttcctct ttttcgatgc cagccgttgt
    26521 tgcaagcact acatcatcaa tatatacgaa gtctctgctt tcatttccat cttcaaaaat
    26581 attgatgtct ttgtgttgta aaatccgagt ggaaaaaatt gataatatgc cagtatatgg
    26641 attgctaagc gattgacctg gtccataaac attttgatag cgaaatgcta cgcatggaat
    26701 gttcaacgat tggcagcaaa tcatcaacgc ttgttcttga tgatatttag ttaaaccata
    26761 aatcgacttt gcttgtattt tactttcttc atctgtggcg actactttca aaaattcatc
    26821 ggcattattt ggatttctga gttcaaatat gccgttttgt aagtcctgct ctgcacgatc
    26881 ttttggataa taatattgtt gttctgtttc tgagaaatat tttccttcgc catatacggc
    26941 acgtgaactt gctataatta ctttttcaat atggtgtttt tcattcgcca aataatcgag
    27001 taatagtgcc gtgccttgta cgttgacgtg cgtatatctc gatatctcat acatgctttg
    27061 acctgttcct gtttctgctg caaagtgtac gacgatatgt tgattttgca atgcactatg
    27121 ccaatcactt ttgttgctga catcaccttt tataaatcgt actttttctt ttatggattg
    27181 ataaatcgga gaagttttat caggattatc gccatgaatt tgcggatgta aattatctaa
    27241 aacagtaata gcatatcctt tttgtaataa atgtaaggat aggttggagc ctataaatcc
    27301 tgcgccgcca gtaatgagta tatttttcaa ataatttacg attgtatgct tcaaaaatag
    27361 ctaaaataat acaaagaata cacagtgctt ttaagcacgt acaatcattt taaatgtatg
    27421 ccaaaatgta gttctataaa atttgttttg caaaaaaatc agtttacgtc gtatgccttt
    27481 tgtcgtttct aaagcaataa aattttgaat ggccaattgc acatgttcac tagctttagc
    27541 acctattctg ctttgcagtg cttttgcttg ctgcacacga atgcgaataa aatattcata
    27601 catgttgcta gaaatcgcat tattagaata tcgtttcatt ttatcgctga aattggtttg
    27661 ttttcgcgtt acattttccg aatgatttct gagtaataaa agtggttctt caatggcaat
    27721 tgcttgtccg caagtagctg ctaataaagc tatccaatgg tcgtgtagta atgctgtttc
    27781 cggaatcgga aatgccatat cgcgcatcgc tttattgatg agcattgtgc agccatgcgg
    27841 aatattttga atcaacaatc tgtttaaagt aaaatatttt ggacttaagt gcaattgttt
    27901 ccaaactgat tttgcagtta cctcgccgag gtcattaatg cttttcatat cagcatatat
    27961 taaacaaggc atatttttat ccatttcaat agtcgaaata agtgctaatg atttttccat
    28021 tttatgcggc aaccaaatgt catcttggtc acaaaaaaaa atataatcag catttgtatt
    28081 tgctaataat aaattgaaat tttgtgtggc acctacattc tttttttcat cagtaataat
    28141 ttttatttta tcagcatatt taagttgata ttcttgtata atatgtagtg tattgtctgt
    28201 cgattgatca tcgcgcacca gcacgctgaa atcttgaaac gtttgattaa aaagactgtc
    28261 taattgttcg tgcaaaaagc gttcgccatt atacgttgcc aataatattt gtacggtttg
    28321 ctgcattata attgatgata gccaagtgtt tgcattaaag ggaattgttt tattttttga
    28381 tagtatgctg tataatcttc attcgacaga aaagcatacg ctctgtctgt tttgatactc
    28441 gcaatttctt tagctatttg ttcatctgtc gtttgcaatc cgcagtattc acaaagctgt
    28501 tgcaactgtt ctttagggtt ggataaaaat tcttcgtatt ttatggaaat ataattttca
    28561 tgagtatgtt gtaatgacaa agcagtgctt acatattcct gccaaagttc atagccttgt
    28621 tctatattgt acaacctgaa attttcatga aagtttttgc ttacaagaaa ttgccttttt
    28681 aatttctttt tccaattcca ttcaaactta ttttttaata ccaaatcgcg ttcgatatag
    28741 cttgacacag agtcgatagg atttctataa atatgtataa tttttgggtt tggaaaaata
    28801 tgcttccaga aatctatagt gaatgtattt tttggatctt tccaaccaaa aggaaaatca
    28861 atgtctttta tattctgata agcagatgca tgtttgccaa gatattgctt acgtttgctt
    28921 gattgtataa aatactccaa tgactcataa attacttgcg cacaagccgg atttttatag
    28981 cgtaaattat gtggtttttc aggtgtagcc gtatgaatat caaaaatcca attattaatt
    29041 tcccaaaaaa ataaagcctc gtgattgatt tctttttcct tgcctacaaa caagcctaaa
    29101 ttttctaaaa gctttgtaat catcgtcgtg ccgctacgat gcatacctaa aataatgact
    29161 ggctgttgtg acatctgcac taagataaac ataaactttt taacaacata aaacaagcta
    29221 taatctaatg aaattagtag cgtcatttta tgttgatgat tttgtaaaaa tgctgtttca
    29281 taaaaaaaat ctgtgtatct atgtgataaa atggaaaagg tactcgaaat attgtatgaa
    29341 gacaatcaca tcattgccgt taataaacgt agtggtgaca ttgttcaagc agatgaaact
    29401 ggagacacac ccttgtcgga aatcgttaaa tcatatatta aagagaaata ccaaaaacca
    29461 ggtgatgtgt ttttaggtgt ggtgcatcgc atcgacaggc ctgtttcagg cattgtattg
    29521 tttgctcgca catcaaaggc gctgacacga ttaaatgaac tttttaaaaa caaaactatt
    29581 caaaaaacgt attggtgtgt ggtgaagcaa aagcctaagt taaatgaagc gacattgcaa
    29641 cattaccata taaaagatgc gaaacagcgc aaggcaaaat tatatgataa agaagtagca
    29701 cattccaaaa agtgtgtatt gcattataaa ttattggtga gttctgataa ctatcattta
    29761 ttagccgttc agttagaaac aggtagattt catcaaattc gtgcacagtt ggcaaaaata
    29821 ggctcgtcta taaaaggcga tgtgaaatac ggatttgagc gtccaaacga aaacgcgaga
    29881 agcatacatt tacatgcacg aaaaattgaa tttgaacatc ctgttaaaaa ggagaaaata
    29941 gaaatcatag caccgctacc tgatgataaa atatggaaat attttgacga aaaaatgcta
    30001 cactatcagc aataaatacc acagatttgg tatttttttg aaaacaatct tttttagatt
    30061 tataaaaaaa tattcaatga aaaattcaat tttttgtatt gccattattg ccatattttt
    30121 tacggcttgt aagaaagagg aatcggctac tgcagacgta aaaggcactt ggaatcttca
    30181 actttgggac ggcgttccag caagcggaac cttagttttt actgctacaa ccttagattt
    30241 caattgtagc acgtatagta tttccgaaca aaccacttac acgaactcag gtaacacgta
    30301 tgtttttact aaaacaggcg gaccttctgc agttattgcc gcaggtaata actggcacat
    30361 ggacacgctt actgctaatg tattgcgaat gacgagtcat tttggtttga tagtaagagc
    30421 aacaaaataa gattatccac gttgtcttaa ggcttcaaat aaaacaatag ctactgtatt
    30481 ggaaacattt agcgagtcgt tttgtccgcg cataggaatg atgatattct gtctagaatg
    30541 attttcccaa aaggcatcaa caccagtggc ttccgtgccg aacacaaaag cacttgcttg
    30601 tttataatca cagtgtaaat agttttgcgc agcctttaat gaagtggtaa aaatatttac
    30661 tttattgttt tctaaaaact gtaaggctgc ttctttttcg cagataataa tttgttgggt
    30721 aaatatacaa ccaacagatg agcgtattac attcggattg taaatgtctg tttttgtgtc
    30781 gcaacaaata attgcagtaa tatccgttgc atcggcgatg cgtaaaatag caccgagatt
    30841 gcctggtttt tcaatgccgt caagtattaa aaaaacagga tttttttcct tttctatgct
    30901 ggataattga tgcgattttt gttgtgcaat cgcaagtact ttactcgtat ttccacgata
    30961 agaaatttta tcaaaaacat catcagtcac aatgatgctt tctatgttat aggaaagcgg
    31021 tgctattgta tttttgaatt cttcagacca tgaggtatga taggcagatt gctgacataa
    31081 aatggtttca atcgaaaagc cattttccaa tgccatttta gtttctttcc agccatcgat
    31141 aacaaaaata ttttcttctt tgcgcgcacg agatttctct tgcaagtgca ctattttttt
    31201 gattaatgga ttagcactgg agctgatttc tttcacctta caaaaatgaa aaattatttt
    31261 cataaattag ataatgaaat ctacaaaaga gataattcga tgtggctggt gcgtaggcaa
    31321 acccttgtat gaacaatacc atgatgacga atggggaaaa cctgttcatg acgataaaaa
    31381 acagtttgaa tttttagtat tagaaagcgc acaagcaggt ttaagctggt acacaattct
    31441 acagcgaaga gaagcttatc gcaaagcatt cgccaatttc gattataaaa aagttgccaa
    31501 atatgatgac aaaaaagtgg aactattatt acaagatgca agtataattc gcaataaagc
    31561 aaaaattaca gcagccataa ataatgcaaa gaaatttata gctattcaaa aagaattcgg
    31621 tagtttttct gcatacattt ggaattttgt cggtggcaaa cctattgtga ataaatggca
    31681 atcattaaaa gaagtaccag ccaccacaat tgaatccgat gctttggcaa aagacatgaa
    31741 aaagagaggc tttcagtttt taggttctac aacattgtat gcgcatatgc aggcgacagg
    31801 cttggtaaac gatcatttgc tgacgtgtca cgcatataaa aaatgcaaat agctatgctt
    31861 tcatttttat ataatcattt ttagaaatgc catatcgata tgtatcaaca gtcatattgt
    31921 cgtaagtagt gtatgcatta taaaacgcac cttcatactt taatcctgat ttttctacca
    31981 ccttctgact tgataaatta tcgataacga tacgtgcaaa tatgtgttcg aagggaagat
    32041 aagtaaaaca caatcgtatt atcaattgca tagcttctgt aatcaagcct tttttctcat
    32101 aatcaatgtc gataaaatat gctaattcgg cttctttctt tttccagtcg atatctttaa
    32161 tattaaagtg tccaattaat gcttgtgagt ttttgtcaat aagtaggtat gcgaatgctt
    32221 tgttattatt ccaatcaaat gatttttgtt gcacatagtc ttttgtgttc tccatcgttt
    32281 gtgtcgcctt caacatattt gggaaactgt gcattagtcg tgctttgttt ttttgaatta
    32341 atacatacac ttctttatac aagttgatgt gataaggctg aagcagtagt ctttcggttt
    32401 caattatttt ttttggcagt tttgtcattt atatatttat gtaataaaaa gaaaatacta
    32461 tatttaatta caccaagcag aaacaagata atgaacttag cgtacttttc agaaaattcc
    32521 atattacctt tggtaataac gcctacagaa ggcgaatcga aaaaagaaac tttagtacag
    32581 tggattgctg ataacaaagc ttcagtagaa agcaagctta cagattcagg tgcgatatta
    32641 tttcgtggtt ttcaaattaa tacgccgcaa gattttgaag atgtggcaaa agccatagat
    32701 gccgatttga aagatgacta tatgggcact tcgccgcgcg acaaaaaatc ggaatatgcg
    32761 ttttctgcca gcgagttgcc agcacattat cctattatgc aacattgtga aatgagcttt
    32821 ttaccttctg ctccacgccg tttatttttc tattgccatg tagaacctga atatggcggt
    32881 gaaacgccaa tttgcgattt tagaaaagta tatcaagatt tagacccgaa aataagagct
    32941 gaatttgaaa ggaaaggcgt aaaacatatt cgcaattatt catctccaaa tgaagcagca
    33001 agaagtggct ttcagttgaa aaaatggacg gatttatttc atacaacaga taaaaaagta
    33061 attgaagaaa aatgtaaaga aaatgatatt atctgtgaat ggaaagcaaa tgatggattg
    33121 cgcatgacca ataaaaatgc tggaataaaa gagcatcctg tcacaggcga gaaagtttgg
    33181 tttaaccaca cacaagtgtt tcatatcagc gcagctgccg acgaatatta tcgcattcac
    33241 aaacgacaaa gacgatggga aacatttaaa accagtatgt ttttagagtt aatgacttgg
    33301 tataaaaagc taacaaaaaa cccgaatgaa caaagcatgc atgtgacttt tggtgatgac
    33361 tcggaaattc ctgatgacta tgtagagcat atacaagaag tgatatggaa acacatggta
    33421 attcaagcat ggaaaaaagg cgatatcatt tgtatagata atttttccac atcacatgga
    33481 cgattgcctt attatggacc tcgtgaaatt atggtttgct ggacatcgta aaataaggct
    33541 atttttttag tataatattg catgaattca ttagaaaaca ttcagataaa tggacatggc
    33601 gacagtttgt tgctactgaa aattattttg gcatttatat tgttttcttt agcaattgat
    33661 atcaaaaaag aagattttaa agtcttattt tccaatccaa agtccgtcat tattggctgt
    33721 atatcgcaga taattatctt gccacttttt acttttattc taattcttat cttgaaacca
    33781 tctgcatcaa ttgccatggg catgctttta gttgccgcct gtccttgtgg caatatgtcc
    33841 acttatgttt cttacttagc aaaaggattt ataccattgt cgatttctat cacagcgatt
    33901 tctaccttat tggcaggaat tactactcca ttcaattttt atttttatgc atcacaatat
    33961 gcaccagcca aagccgtgct acacgaaata catatttcat ttttgtattt actcagctct
    34021 atagccttgt tgttattatt gcctttgtcc gcaggaataa cattaaaaca atattttcca
    34081 tcgtttatca atcgaataaa caagccagta aagttattag cattgttatt tttcattgca
    34141 tttttagttg gcgcatttat taccaatagc aaaattttca tagagaattt atctcagata
    34201 ttttggtttg ttttcttgca aaatacactt gcatttttca ttggctatgt atttccaaaa
    34261 ttattcaaat tgccagcgtc gcattgtaga agtatttcta ttgagacagg aatccacaat
    34321 tcaggacttg gtttgatatt agtgttagcc ttttttaagg gaaatggtgc aatggcatta
    34381 attgcagcat ggtggggaat ttggcacata attgcaggca tggcaattgc ttggtattgg
    34441 aataaacaac tacgttaacg gcaaattagc aagcttgagt tcgggctttt tctttattgg
    34501 ttttttcttt agttcaggaa ttgcagtttt atctttgtcg gccaaaatca acaaggcgat
    34561 gaaatagaat ggcagtaatg gaattttata gcgcaccaaa gaaccaaaat tataactcgt
    34621 aaatccaacc gaaaaagcaa atataagcga aaacactaag cagaattgca cttcaggatt
    34681 tgttagaatc aatcgcgtaa atcgtataaa ccctgtttta tacaaaagcc gaatggtgaa
    34741 aaataaaaat agtaaacctt caatcgctgc tggaattaaa ataggttttt tcgcctccca
    34801 taaataaggc cgaaaaatag acacattaat agccttagga acaactttaa taattcctgt
    34861 tagtgtataa tcaatattgc ccaacgaata gccagaacca ccttgtttta aggaggaata
    34921 atagagccag ttttgtgtgt cttttgcagt gcgcacaata ctttcaaaag aataacgctg
    34981 agaatatgtt gcaattttat gtaatataaa tacaccaata atcgttgcaa aaacaaaaat
    35041 tacaggacca aacattgatt ttaaaagatt actgccaatt gtttctttat atcggaatgc
    35101 gatccagaat gaatatgctg gtagaaaaga cagcaatata tacaccttta tcaccttcaa
    35161 aagataaaga caaataaaga tgaaaattaa acgtcgaaat atttttgttt tttcaaagaa
    35221 tagcttgtaa acatgatatg tcagcgcgcc caatgcgccc atacaaatcg gatcttttaa
    35281 gatgcctgtt ccccaaaaac atacagacgg caaaaacaag caagcaacag caaatccttt
    35341 ctcgagatgc ggataaagtt gactaaataa agaaaatatc ttccagcatc cataaaaaca
    35401 aaacaatgta tatacaaaag agattagtag atatgtattg aataacggat agctgagcag
    35461 tgttgttatt ctaattacga gactagagct ttcattgagg tagaatgcac taccaacgcc
    35521 taaatatttc tctaccaaaa aaacatcatc tggcgtaaaa aataataatt tcaaatagtc
    35581 aatcggattt tcgtgtaata cctcacgcaa tcgaagtgaa tatgtcaaat aagtatgcgt
    35641 gtcgccaccg ccttcgtaat ataattgata aaccaatgcc attaatattg cacaaacaaa
    35701 ttttacatat agtgcaggca aaaaatactt tttatatgca ggattttttt tcttttttat
    35761 atatcgctta gcaagaataa tggtgattaa aataaaaatt ggtgctaata ataaatcgtt
    35821 tagaaataaa aattgtacag gatcatcatg tgtaaaccac ttatccattc attaatatct
    35881 ttatatttac agcgatgtat ttggaatgat gtataaatat aaatatttcc ttgctctttt
    35941 cactattatt gccatacttc ttttatggtt ttactttcca atttgttttg acaacaatga
    36001 tgatcaggca atgtttgcca ttagttctgg ctggctcagc ggcgtgcctt ctgctaacgt
    36061 aatgctatct aatgtgttat atggtaaatt actaagcact ttattttctt taaataatag
    36121 tataaattgg tacactctta ttttgcaatt aatttttgcg atttgttggg tttatcttct
    36181 ctgttatttt ctttttctca agaaaaatac gctaataatt tctgtattta taagtgctat
    36241 tttgttgtta ggatttattg tcgctgcaat tacacgtcca cagtttacat ctgttgcatt
    36301 atcctgtatg gttgcggctt tgtttacgct gtatcaatca caacatacaa catatagatt
    36361 tgcatggttt gtgtttttta ttattgtggc aatacttatt cgggcagaat ctttttttat
    36421 ttttcttgtt tttgcattgc caatatattt ttggaacaaa tccaatagaa aacaaatact
    36481 tcgatggata gctatatcaa tattgctttt ctttgttttt tattttgtgc attatgtatt
    36541 cgctattcgg ccacatgagc aaaattatat gactttgcaa acaattgata tcatcgcagc
    36601 aaagcccatt tctataaagg aaaaaatatt atttcaaaat aatttttcag tacaagatat
    36661 acacttgatt caatcatggt tgatggctga tgatgcatat aacacgcaga aaatgcatgc
    36721 tttggcaacg gcattgcagt cgaatagaaa ttgtgctcaa gtattttttg aatggaaaaa
    36781 gtttgcgcaa gaagaacgtt atttacttgt tttttatctt tttagcttga tagcaatcct
    36841 gttttttgct aaaaaatatc gaaaatttgt actgtggaat ttaatattgt ttttaactgt
    36901 tgttctttat ttgtcaatta catcacgcat tccacatcgt gtgacttatc cattactttg
    36961 ctatcttgtt ttgttcaata ttttccatat tatcaaaagt gattttgtaa ttaaaataaa
    37021 atatgcaatc ctgagtatat tcttactttt gagtttatat aaattttatt gtgtactact
    37081 attaattcca ttacaccaat cgtatcaacg acaatttgaa aaaacagtat cattaattcg
    37141 ccaacatgat gacctgttgt ttattggtgc tgatgcattt ccattgcaat atatgaatgc
    37201 atggcaagcg ccttctcctt cactattggg aaataatctt atgctggctg gtatgcaacc
    37261 acaccagatt accaattact attatcacaa catcagctaa aaaatctgac aaatgcatta
    37321 tctaaaaaat caaatgtgat atttctgagc gcttcgtctt ccttgcaagc tgcttatatc
    37381 caagtgatga aagaacgata tggaattgca tgtcatttcg aaccaataat aaataaaggc
    37441 tatccagtcg attcgaaacg attagtgttt gatgcttatt gagtaatgtt tttgtaaacg
    37501 cttacatatt tttccacacc tttttctaaa gaaaaatagt cgtgcgcacc ttttataatt
    37561 tgtgcagtgt cgaatttatt gtctatttgt tcgatagcgt ttatgtattc agcttgtgta
    37621 aaagcattaa caagaatacc acttttaaaa tctatgacca ctttgtctgt atctccaaca
    37681 ttggtgttgc acacgattgg aattcccatg gccatcagtt cgccttgctt ggttggtgat
    37741 gatgcttttt tagaataaga aggtaaaata aaaaatatcg ataattggca aagcgcaatt
    37801 agataaggta tctcttctct tattccaggt cgaataataa tagcatcttc gacacgatat
    37861 tttgcagcag tttgcttgat acggacatgc tcttcttttg ttacaaataa gaatttagca
    37921 tgttcttttt tttgttttaa tacactaaaa aattccatca tctcatctag catgtaccat
    37981 gtgccaatag agcctaaata cagtagcaca taatcacctt gtctgatttg taattcgttt
    38041 tgtagcgtat atagtttttc ttgttgtatc tgtgttttgt tgaagagctg caaatccaca
    38101 caacatggaa tcacttcaat ttttatagga ttgtttttga tgtttttcca tgataaaatt
    38161 tcctcttttg cattatgtgt caaactaatt gtataatctg cattttctaa gaactcaatt
    38221 tcttttttct tgaaataatt aaaaaccagc ttgtatatag gattgtttaa atgccagata
    38281 ttgccatcaa cacgctcatt tgcccaaaat ccacgcatat caaaaatgaa cttcagtcct
    38341 aattttttct ttaactgcaa acctactaac gccgcgatat agcttctgca atgtataata
    38401 gaaaaatggt gtttatgatg taattgcgta gctaattttt ttatacgaaa tacatcgtat
    38461 aatgttgaca gaattggcgg ttttttggta tagctaatcg ggtgccactg tatgttattc
    38521 tcagataata atgtcgcaat ttttgattga tatagtgcga agttttcagc tttttcacag
    38581 ctgattaatg aaatatgata tccatttttc gtcaaaccaa tcaaataggg aattacttgc
    38641 gactgaccaa gtggatctgt catgccatca tacgaaatat atagactatt aataggcatt
    38701 atgcgttttc ttctaaccac caatgtaata ttacaagact ccaaattctt gcatataaat
    38761 aatcttcacc agcaaaaaat ctgtttttta aatgcgctac atgtagatta gaaacgatat
    38821 catatctatt aataacaatt ggcgataagt atttgtcgat taagaattta aagtttgcac
    38881 ttaaccaatg tgacaatggc atagcaaatc cttttttagg tcgattaaat aattctttag
    38941 gcagatattt gtaaagaatt ttcttgagga tatattttgc ttcgccgttt ttgtatttta
    39001 agtgttcgtt gatgtttatg gtgtaatcta ttacatcatt atccaaaaaa ggcacacgac
    39061 tttccaaaga atacagcatt gttgctctgt caactttcac caataaatca tcttttagat
    39121 agtattgtaa atcccaaaac gactgacgtt cttttgaaga taagtttctt tttgatgtgg
    39181 caatggcatt gatatcatca aaattaaaag taggattgac taggtagtgt ttcaaatctt
    39241 tttcgctaaa aagatattgc tcttgcgaaa atatatgcga ggctacatgg tcatgattgt
    39301 cgtaatttaa taaccaagca gcacgtttat accgaaatga catacactgc gatgccatat
    39361 agaaaggttt tcgaaatacg cgaacaaaag gattgtctaa acggcttgcc cattgatgca
    39421 taccatagcc catgaacaac tcatcgccac catcgccact cagtgaaact gttacttgtt
    39481 ttttagcttg tttagaaaca aacatagttg gaaaggcaga gctatcggca aatggctcgt
    39541 cgtaggtgtc aataatttca tgtaaaatct cttccaaatc aacttcgccc attcgataag
    39601 caaagtggtc agtgcctaat tcttttgcta ccttttcagc ataaattgtt tcatcaaagt
    39661 gactgttttg aaagccaatg ctaaaggttt ttattttatc tttcttgatt tttgatgcaa
    39721 gtgcagttat caaacttgag tcgatgccgc cactcaaaaa tgtacctaat ggcacgtcgc
    39781 ttatcatttg tgatgctatg gaagcaataa gcaattcttc caattttttt tctacttcag
    39841 cttcattgct atgcgtgttt ggtaatactt gctgatgcaa gttccaatat gctgtgaatg
    39901 gaaatattgt attattgttt aaagatttga catcaatttc tacaaaattt ccagcaggaa
    39961 acttccacac ttgctgaaac gcagtgtctg gctctgttaa aaaaccgata tgcaaaaatt
    40021 cggcaagtgc tgcattgttt attggcatat gtaaatcagg cattgccttt aatgccttta
    40081 tttccgaggc aaaagcgaac tgtatgccgt cgaaataata gtaaagtggt ttaataccga
    40141 ttctgtctct acacaaataa agtttgtctg catgaatgtc ataaatagaa aatgcaaaaa
    40201 tgccattgag caaagtaaaa gccgctgttc caatctttgc aaatgcttct atgataactt
    40261 ctgtgtcgcc actcgttttt agtgtaagct gtaaatcgcg tgcaatttct ttaaaattgt
    40321 aaatttcacc gttataatta attacataac gaccgcatgc cgagtagaac ggttgagcag
    40381 aagcatctga taagtcaatt atttttaaac gacgatggcc aagtgcaagg gtgttgtttt
    40441 cattaaaata aaatccaaaa gactcaggcc cacgatgtgc gagtttattt gttgcctgtt
    40501 taaaggcatc tttgtctaaa gatttattta aggaaagcca tccgcttatg ccacacatat
    40561 actttgtatt attttatgac catttttcta aaattttttg aataccatct tctaaggaaa
    40621 ccaaaggtct attgttcagt aatgttttca tgttagaaat atcaggaaga cgtcgagtca
    40681 tatcgccttc tttcaatgct ggcagatgta taatgtttga tttagaattg gtgatttcta
    40741 taattttctt ggcaatatcc aataccgttg tttcattgtc atttccgata ttgatagtcg
    40801 tattttccca gtttgaagat aaaaaggcat ttatagttgc ttcgatatta tcatcgatat
    40861 agcataaagt tctactttgt gagccatcgc catagatagg aatatctttg ttttgcaaag
    40921 caagtttaat aaattttgcc acaacgaaat cctgcgattg attagggccg tatgtattga
    40981 aaaagcggaa aatagtataa tctaaattaa actcttgctt gtaagatttt agaaaagcct
    41041 ctccgatatt ttttacgatg gcatacggaa gctttgaatt gagtggcgtt gtttgttcat
    41101 tctgaggaaa ctctacaggc tcgccataca cttctgatga ggaagagtag aacacgcgtt
    41161 ttacaggcgt atttttagag agattcaaga tatttttaat gccttctata tcgtgcaata
    41221 cactgacagg attatctaag gtgcgttgca cgccaacagt agcagcataa tggaatacat
    41281 agtcgatatt atacgataaa aagataggca taatatcgtc gtaaatattt gcatcgcatt
    41341 ttataaactt ccaattgttg ccaatagtgg gcagtttttc aattgtgcca gtagataagt
    41401 tatcgacgat aatgacataa ttattagcat cttgaattag tctgtgtact aaagcacttc
    41461 ctacatttcc agcaccgcca gttactaaaa ttctattatt gtattgtaaa aaagacttca
    41521 ttttatgtga aattagtaga tgattaaaca ttttatgatt ccatatacgc gtatatcgaa
    41581 tattgataat gggtgtattg tcaatgcttt ccaaaaataa gataaggcag tttttttatg
    41641 gctagacatg gcaagatgta agacaacgta gagatagcat ttggcagtaa atttatctaa
    41701 ttgtccagca tacaccttct gtatcattgg atcttgtgtg caagtttgaa tgagcaagtt
    41761 tattcttgca ataagtggtt ctttttttat catcaacaca cttctttcgt cgtggtttaa
    41821 aattgtgttg gtgatgacat cagaataata aatggtataa cgtgcggcaa ggcgcaacca
    41881 caaatcgtaa tcctcggagg cagacagtgc tcgtgtttca ttaaaaggat gctgcaatgc
    41941 cacatctctt cttaaaaaaa caccattaca acttagataa tttccattta ttaaatcttt
    42001 attgaagttg gtgcctttgg caattttttg ttctttaatt actttatgat taatgtcctt
    42061 gacattatat gccaaataaa tcatctcagg attattgtgc ttttctataa gctttatggc
    42121 ttctgatagg tggtttggat atagataatc atcggagtcg aaaaaggtga cataatcgcc
    42181 agaggcaagc tgcgtacctc tgtttctggc agcagcacgc tcttcattta t
//
LOCUS       DAOQQV010000008        39975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_277_length_39975_cov_9.001954, whole genome shotgun sequence.
ACCESSION   DAOQQV010000008 DAOQQV010000000
VERSION     DAOQQV010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 39975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39975
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_277_length_39975_cov_9.001954"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLL59_01760"
     CDS             <1..279
                     /locus_tag="PLL59_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HMY22556.1"
                     /translation="APSAELRDGQKDSDSLPDYAILDKVLFQYIEQHQGPDELEAMGF
                     DEKLITRILRMVNRNEFKRHQTPPILRVSPKAFGSGRRLPIVGKYLES"
     gene            284..1147
                     /locus_tag="PLL59_01765"
     CDS             284..1147
                     /locus_tag="PLL59_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22557.1"
                     /translation="MHIHLIKKQTINYPSGSAMEFYNNILYIIGDDVNYILCLDEAWK
                     EIKRIELFAFTGTRIPKPEKPDLECATIIGNKLYVVGSGSLSPQRDVAFIVYLEEDKV
                     KRISTAAFYALFRDRNLVAEMNIEGFTDCKDKLLFFNRANTQQANQLLITDHKIIKKQ
                     FPDKFKVLPVEIANLRQLPLGISGACYDDKNDILFLSASAENTSNAYDDGEIEGSAIG
                     VVFNAYQKLNDKKYTIDKLICLDDVHQDFQQQKIESICILRQSNNTYRMAMVADNDDG
                     KSVLFEVEITL"
     gene            complement(1195..3804)
                     /locus_tag="PLL59_01770"
     CDS             complement(1195..3804)
                     /locus_tag="PLL59_01770"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HMY22558.1"
                     /translation="MKLFQRITILASIFLLLSMNIFAKKEKKKTDKDTVKTMTDSLKT
                     TAKDSTSKKDSKPQFKAIKEVTEKCRKTAGLFPIYQDTTNGKTYLEISEEKIGKEYIY
                     FAYVLDGYLDVGFARGGYKDNSIFKVEKYFDRIDFVLQNTNYYFDEKNNLSKAKDANI
                     NQSLFLSEKIVAATGKTYLIDADNLFLSESVTQIKPSKMPGERPDAFSLGTLNSKKSK
                     YLAVKNYPDNTAVSVEYVFDNLAPANYGSNEVTDARFVSVKMQHNLIEVPNNDFRPRA
                     DDPRVGYFLEKVDDQTSASATPWKDLIHKWNLQKKNPEEKLSEPVKPIVWWIEKTTPA
                     EFKPIIKNAVLAWNEAFEQAGFKNAVACYEQPDTASWEAEDIRYNVLRWTSSPRPPFG
                     GYGPSFVNPRTGEILGADIMLEFIYMTNRLPLEKLYDIAALDHMQSENPLQHSNCAFG
                     ETMHQNVLYGTQLLAANGFSDIDKDEFLKQALYDLVLHEIGHTFGLMHNFIASNLHTN
                     DEMQDPVLGATVGLTASVMDYTIPNISPDKNKQGLFFDVKPGLYDKWAIQYGYTNIAN
                     EDEENTALKKILSESDKRAYRFRNDADDMRSTGKGIDPRGNLFDMSSDAVAYAVSSIK
                     MVNDAMPKLLTKYSTTGNSYHELRKAYLSLSGHYARSLNVISRYIGGVYVNRNFVGQD
                     AKEKPFTPIPLSEQKNAMQAITKYAFSKNAFTAPTELYNFLQMQRRGQDFRKETEDPK
                     IHERILNIQRGILDQLLNSTVLQRITDTKLYGNKYTLNAVLKDITNGIFIEDLGASVS
                     SIRQNLQEEYVSRLLSIVDTKSAYAYTSKSAAFGEVKRILDWMNAYSGGDAATKAHRA
                     HLAFVINKALEDK"
     gene            complement(3909..4244)
                     /locus_tag="PLL59_01775"
     CDS             complement(3909..4244)
                     /locus_tag="PLL59_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HMY22559.1"
                     /translation="MKTKILSIASAIILFFSGNMMAKDTLTKASFKVSGNCDMCKERI
                     EKAAKSAGVKHADWSEDTHILTVSFAVEKTNLEKIQQNVANAGYDNEKFKATEEAYKK
                     LPKCCQYDK"
     gene            complement(4256..4567)
                     /locus_tag="PLL59_01780"
     CDS             complement(4256..4567)
                     /locus_tag="PLL59_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22560.1"
                     /translation="MKQTTTILALVMVLAIAIFGFTACKNNCKDGEKCEQKMSCCKDG
                     DKCCDKCSNDCTADKKCCDKCQAGEEKACCKKDEKDCSKDSTAASGKKEKCCKEDSTK
                     H"
     gene            complement(4588..6786)
                     /locus_tag="PLL59_01785"
     CDS             complement(4588..6786)
                     /locus_tag="PLL59_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY22561.1"
                     /translation="MKIINNWVFAFVFVAFQYTGFAQEKQKIKIKVSDRTTGEMLYGA
                     NVQWLDTTIGATTDEYGIAELNVAGALPKKIIVSYIGYQDDTIEVHHQTEMNITLDPA
                     IALEEVKIKGKRQTNFISSINPLKTEMIDAGELKKAACCNLSESFQTNASVDVNYSDA
                     VTGAKEIKLLGLNGTYVQNLLEGIPVMRGVTATFGLDNIPAPWIKSISVSKGVPSVKN
                     SYEGITGSMNVEFKNSFNDSTKLFFDMFGNQNARIETNLLINHKINKKLGTMLMANGA
                     FVPVKQDMNHDGFLDQPLMKQYNLLNRWNYHGENIEGQYMVKVLSENRTAGQITAHHE
                     NDTLPLFGIGIKTKRVEAFAKTGYIFNTSKNNSIGTQFSGVYHQQNSNYGQQLFNTKQ
                     ASFTGTVLYQTDIKNENHTFVAGANFIYDKITEKLDTIQFNRNDFVPGIFAEYTYKWT
                     NRVTLVTGLRGDYHTRGGFQVNPRIHAKFSLTNNTTLRVAAGSGFRVPNILSENQSLL
                     ASSRTIVFQDFPSKEIAWNYGISLTQKFFLKNREANFTVDFFRTDFTKQAIVDIDNAD
                     GLATISNLKGKSFSNSLLLELNMEVAKGLDVKMAYRLEDVRATYHGKLLQKPLQALNK
                     GLIALSYKTPNEQWQFDINTSINGKRRLPNSFEGDAGKRFSPIYALLNAQIMKIFKHG
                     EIFIGCENITNYRQKNVILGTPFSTVFDTYQVYAPIMGANVYGGFRLFIK"
     gene            complement(6835..7275)
                     /locus_tag="PLL59_01790"
     CDS             complement(6835..7275)
                     /locus_tag="PLL59_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22562.1"
                     /translation="MNKRINISALLLALLVLVSSNGIALFEHICDSSKSKDYSFFSEI
                     NCEKEVKTTSCCNKPAIEESSDCCSHSQFFSKLSIEGFTAKQLKINSVEDFLIPFFNA
                     LAINILPSYIAQLHTGLPPPDNLYTTIIKSHLAPSSVKLQVFRC"
     gene            7401..8231
                     /locus_tag="PLL59_01795"
     CDS             7401..8231
                     /locus_tag="PLL59_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMY22563.1"
                     /translation="MTFKEIIQQITNPKEVTQSHEFISKFPNKVGSYGYDAWGFNING
                     IKPFVGMGRFFYENFFRVEVHGLENIPKEGRCLIIANHSGQLPIDGMMLGYAVVTNPH
                     APRAPKGMYERFVPQVPFISSIFSQWGGTVGDPENCIKMLENDEAVIVFPEGARGISK
                     SYKKKYQLQRFGLGFMYMAIQTKSPIIPVGMVGFEESMINFGNIDFLERFLKFPAAPA
                     LVPFVFPSKVIINIGKPMTFEGDISREYQVNEKVDEVKKEINRLIDVGLQQRKGIFSK
                     "
     gene            complement(8285..10714)
                     /gene="lon"
                     /locus_tag="PLL59_01800"
     CDS             complement(8285..10714)
                     /gene="lon"
                     /locus_tag="PLL59_01800"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HMY22564.1"
                     /translation="MFQKIELQNIIEEDVEMVPLLSIEEDDNTNEENLPKVLPILPLR
                     NNVLLPGVVIPISVGREKSIKSVKKAYKGNKYIGVIAQRDIKVEEPEPSDLYTIGTIA
                     RIIKMLSMPDGGNTLILQGIRRFAVKQNVTSEPIIESEVEYLADIPMTDKQQEKAMMD
                     NLKDMANKIIELSPNIPNEAKIVLNNINNIKFLANFITSNLNIELAQKQQILETNDTH
                     ERVQQVLKYLHQELQLQELKANIHNKARTDIDKQQREYFLNQQMRAIQEELGGDMYQE
                     ELNRLKEKAKEKKWSEAVAKAFDKETERIKRMNPAAAEYSVILNYLDLMVELPWNETT
                     QDNFDLKRGKKILDEDHYGLDKIKDRILEYLAVLKLKGDMKSPILCFIGPPGVGKTSL
                     GKSIARALDRKYVRMSLGGLHDESEIRGHRKTYIGAMPGRIIQSLKKVQSSNPVFILD
                     EIDKVGKDHRGDPSSALLEVLDPEQNTTFYDNYLELEYDLSKVLFIATANSISEIQPA
                     LRDRMEIIQLSGYSLEEKTEIAKKHLVAKQREAHGLKSNQVKLNDKAIQYLIHNYTYE
                     SGVRELDRVMASIMRNVAKSVATEEEYNVSISTDDITRILGKPKYNHDKYSENNPCGV
                     AIGLAWTPFGGDILFIESSVNKGKGNLMITGNLGNVMKESFNTALTYIKSKADDLKIP
                     AQAFTDWNLHIHVPEGAIPKDGPSAGIAILTALTSLFTQRKIKAYTALSGEITLRGKV
                     LPVGGIKEKILAAKRAGIKEIILCKDNEKDVDEVQSDFIKGLKFHYVETMEEVLLLAL
                     DKKKVNNAIAF"
     gene            complement(10977..11288)
                     /locus_tag="PLL59_01805"
     CDS             complement(10977..11288)
                     /locus_tag="PLL59_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMY22565.1"
                     /translation="MSITKIISEYLEKKGVSQTALGEKMGKNRQYINGLLTKNKDLNS
                     HIIKSISIALEYDFFAELSKELRKEHKEIKSSLNKETNTSEFEDAMIEVLKKRFPNIF
                     R"
     gene            11413..11691
                     /locus_tag="PLL59_01810"
     CDS             11413..11691
                     /locus_tag="PLL59_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22566.1"
                     /translation="MNLNAKTKPKAKQKKYVAITEYRYNIKRRLSNYSANQKSRIMHH
                     VQQETQIGLRTIYNVINEEKGNFKHNNINILLAFARVFNCAIDDLINR"
     gene            11821..12081
                     /locus_tag="PLL59_01815"
     CDS             11821..12081
                     /locus_tag="PLL59_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22567.1"
                     /translation="MKLNEFLEKKQKMHQLNEAICYRKNFIEKNYNVIKFRKMMLNFR
                     REILAFEIKAKHLNEDIEAYEDRFCRLLDIQVLLNSLNVQEK"
     gene            12078..12305
                     /locus_tag="PLL59_01820"
     CDS             12078..12305
                     /locus_tag="PLL59_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22568.1"
                     /translation="MKDSCSMPHVVRRLSDEKLFYMRCCEVFNKDTNEHDIEFQVFKN
                     GKWINDSSICSPNYNFYVPDNEIKNLQLFLL"
     gene            12302..12658
                     /locus_tag="PLL59_01825"
     CDS             12302..12658
                     /locus_tag="PLL59_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22569.1"
                     /translation="MNPNYNQIEFKEYERRMMAVLAKMKIDLDEYKKKENANAAYIQR
                     NENILKHLLEYVAEIVPEYIEILEQQNKNSYRAGYNAGAADAEKKYTWKPDMQDKENF
                     RTYWTLEQKKILPNLY"
     gene            12663..13952
                     /locus_tag="PLL59_01830"
     CDS             12663..13952
                     /locus_tag="PLL59_01830"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE family protein"
                     /protein_id="HMY22570.1"
                     /translation="MSKKIDLEDLNKFSDTAVSYAVEKPKLIRIKEHIFSLYDFRYNK
                     VRLLLEYRKKNEKDWIDFDEDLINDIWINFELNNEFHSKEKPSTRYINTVLFSSFVAK
                     YNPIHDYFSNLKYDGNSYISQLIQCVEIEPLRVNDDYTLDMFFPLLFKRWLVACVACA
                     LGYNANHVLFLLIGGQGTFKTTFLKHLAPKAIDDYCFTGQIDLDIKNKDTLNYLAEKF
                     IINLDDQLQSLYKKDFNVIKTLITASDITNRKAFRRDDKKRKRIANWCGSIDKEKIFL
                     DEKNRRYLSFKIKKIDLAKFQKINIDNVWAEAYDLLKKGERFFFDSEEVNYINQINRL
                     FSIETPEEEWLLALFNNADKTDSPAEVVYLMFNEILSIMQRASGLRMEAKLVTIAMKK
                     LDWSEPISQRIGIDKTPRKVYAVVRKFYEEQGKIIMK"
     gene            14115..14555
                     /locus_tag="PLL59_01835"
     CDS             14115..14555
                     /locus_tag="PLL59_01835"
                     /inference="COORDINATES: protein motif:HMM:NF021634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMY22571.1"
                     /translation="MIVISDTNIIVSALISPNGAVANILNSKRKFKIIAPDFLLDEID
                     KHFDKILINSILTERKELKALISYYKEKITFIPIDEIPQKDRLNAYKLVNDIDIDDLP
                     FVALSIHKKCLLWTSDIKLINGLKKKGFTKVITTSELKKFIYKK"
     gene            14722..15099
                     /locus_tag="PLL59_01840"
     CDS             14722..15099
                     /locus_tag="PLL59_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22572.1"
                     /translation="MQSQKKTATKAQGASNATEQESKPAAAKAYTIFAEPTDDFIFDK
                     LFDEIYQLIQECIKEATPMNANFINETQQKHAIYSILYVCLEKQYPYKTVLQNLQCFF
                     HNIDPEYIMQACTNVYSNRFLTA"
     gene            15494..15949
                     /locus_tag="PLL59_01845"
     CDS             15494..15949
                     /locus_tag="PLL59_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22573.1"
                     /translation="MTTENEILNDLPETETTVSEVETNTANWIKPKMTLSFSEKNATR
                     VQSFVNGLQDSKRVNNISKLFMLLLETAEKQAETESVQFTKMEKQAETISKQAETIAQ
                     LEAQLKHAEANRKQTEYRTETKGAPAVNQQKTNNKPAETTKSGYANFWQ"
     gene            15971..16393
                     /locus_tag="PLL59_01850"
     CDS             15971..16393
                     /locus_tag="PLL59_01850"
                     /inference="COORDINATES: protein motif:HMM:NF037917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22574.1"
                     /translation="MKKEIKNLVQKALTGSNKHMELTINYLPKYQAQIIEKIIGIDLN
                     GVSRSIDTYGIKHTIKEHGSERTELARGQLPVNIDDFELIPEILKTDIVKYGGKNSLK
                     QDVFVFERRIGNLYFVVEAVRFSKKGNKLVFQTLYKRK"
     gene            16498..16848
                     /locus_tag="PLL59_01855"
     CDS             16498..16848
                     /locus_tag="PLL59_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22575.1"
                     /translation="MENKLETQQETVTIPRYVYRRLLDQRSELQQTVIFSTDVILIFV
                     DLLGGKLPQSKKDIILLLPKLPKLIDSLSDEKKRSYLQEAFKNILETAPKYITEKQVE
                     KLKAMDLLPLQENL"
     gene            16852..17487
                     /locus_tag="PLL59_01860"
     CDS             16852..17487
                     /locus_tag="PLL59_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22576.1"
                     /translation="MENTPILTETETIIGPELNEDIFDIETELSKPTQHRSFDFDAVS
                     NEAENMISETETVFNDDIDTEKLKETLENKVADNFEAVYDSSTLAPAIIDGADAIIQQ
                     VFPILYEKTAFSEDERKLIKILAHKQKNKKETTLTDEDLSLMSACAEYENYIESLPFT
                     ENEKKQILKPLSELLKDVNLQTTPGNALIIAVLIIMIPRLMPIGINKFFKN"
     gene            17497..18930
                     /locus_tag="PLL59_01865"
     CDS             17497..18930
                     /locus_tag="PLL59_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22577.1"
                     /translation="MKTLKLFCIAVLMCYATSTSKAQTLQAQHEIVKNLQDRLNQNTE
                     YAGFQTGKQEYFHKFYKYISRTYPGETNSNYINTAAYLTSISASFSSPNFIYDSSFYA
                     LLSLYRNNWQYLSNKYPNTNNNKIIAAAYMVKDIGTEYFSNSLFSSSTAVLDSFIRIN
                     PDYINIDYTEDSILITDFGQNDVPINFLGEYEVEFDWVNGAEVIINENDCNKHFYYKP
                     DLDCIDDTIYYAYKICDSSFNLVNSYNDGENRIVIHTNYDLYQAAITSIDTVLNYYDT
                     LIIDSRNFVNSALPFYVSNDYSNENIIIIDSFSFKYIVPNSIDTVYSFIPIHYSCNDN
                     TFDSLPIFITRPNFANKPFNDDPNLVFDYENAQGRIYWQFVPQTFIDSIQNFTITDAF
                     LIVPNASPEFLQDAVITQSPTDMFVPNASMKNGLQNNPNNMNAILVNATWSRYGINYS
                     GTFTYKLPAFNTSNDFSTTIISTFSEN"
     gene            18920..19471
                     /locus_tag="PLL59_01870"
     CDS             18920..19471
                     /locus_tag="PLL59_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22578.1"
                     /translation="MRINHVNITIGKRGCGKTTKTINLIEKSNLKTLVVDTFDHENYR
                     KFRAIQPVMINDKIWKAGNVRCYGHDFKDIFYQISNKISNAMIVLEDCTKYLKHPLNQ
                     DALRFIVDSKQKNIDLVFMFHGFGMVMPDLFRLADSLTIFKTNENIDTYRNKIPNFDS
                     VKKEFTAIQKSQNKYIHKTIKIN"
     gene            19473..19658
                     /locus_tag="PLL59_01875"
     CDS             19473..19658
                     /locus_tag="PLL59_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22579.1"
                     /translation="MSNFVIKTYSIVELSFKYGVCVRTFKKEIIKANIDIELNKYKKI
                     FFPFEVIKIIEKLGVPE"
     gene            19917..20576
                     /locus_tag="PLL59_01880"
     CDS             19917..20576
                     /locus_tag="PLL59_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22580.1"
                     /translation="MTNSLVPTMADKEYIEKMAHQLSQFDEDNFDDDNDNYVGRVRPK
                     LTQGVMVLNISSSATTTRDFFIFSGYLIDSMSFAATFGSVSTTASYGQIVDGGFKSIG
                     SATADLMAFSDMGASSINDWIAYIKNFKGNKITKIVVRSSNPAVHSQRFVITKKDPLN
                     QLADRTIRLSTWRNPMQQDLNTTIINETIDLGLQDFVKFSIPPSVTDLVIELHMNGYA
                     S"
     gene            20616..21320
                     /locus_tag="PLL59_01885"
     CDS             20616..21320
                     /locus_tag="PLL59_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22581.1"
                     /translation="MDIFNHLATQHRNSVAFVFSKYGIKKPVTPKNVQRAILAGKNTP
                     FYQDLMNAICLQALGKNYYEDDFDYNIENMDTENFEAETVGIIDTFEFDYLDRAGRKA
                     RRKQKKIVRKQKKQAKKASKNSVRPNPNASIQDAEFSDNGSGNFGSSIADKVETGLNV
                     VGQLATAAGGIAGQFLGNKDNDAATADSNSNEDEAATATATANSGVKKYLPYIIGGVA
                     LLAVVGVLIFVLKKKK"
     gene            21323..22267
                     /locus_tag="PLL59_01890"
     CDS             21323..22267
                     /locus_tag="PLL59_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22582.1"
                     /translation="MVFTSVKYVNEFPNGNNSPAAVNRKTGELFINKKRWSNIKAEHR
                     VFILLHELAHCILDTSDEMAVDALAHTWYLQLGYSLSESVFALTKVLHADSDMNKKRS
                     WAQYTRAKEIDNRNDVLNSYDGYAEICSNPCNLITDISNFDDNSILVQYDQYDFDINA
                     ELANVKGLSRKEYRKKKRAARLYKRLGRGKKKHAIANDIQAGADTRKTYADKGMYVPT
                     RAESIAKGVGGTIQGIASAIGGTMGKGAVQSSYTEQEPTEQEQNYSNTNTARKKQTAT
                     AISEPVNNSTTSSNSKKIIIIGGFALMVLGIVVFSIKK"
     gene            22272..22730
                     /locus_tag="PLL59_01895"
     CDS             22272..22730
                     /locus_tag="PLL59_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22583.1"
                     /translation="MKTNYFKIGLYIVGTVVVIAAVFMIIRAFGRAKQGVSSIGDIIQ
                     DNKVNTDIAQAAGVPVQDVDNARKIASDLATELELRKGLTWWEKQKGIVTDSEILDIA
                     SQIKTAAQMRVVAAIFQNELTYNKNLYTELKRELSGSNLSKVPFIETILK"
     gene            22735..23037
                     /locus_tag="PLL59_01900"
     CDS             22735..23037
                     /locus_tag="PLL59_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22584.1"
                     /translation="MKAFITTILTLLFFTATAQDSSAVNNTIHVFSVLELINNAPYWL
                     TTAVIITGALLTATQTILSRFPTEKSVKIKGVIGKILDVLALFPPDNKKGGGTHFK"
     gene            23054..23236
                     /locus_tag="PLL59_01905"
     CDS             23054..23236
                     /locus_tag="PLL59_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22585.1"
                     /translation="MKNYDKITPIEAIGLAQTIIAYLIKGFENIGFRKNTRKDIEALK
                     ELALLQEKRIQALENK"
     gene            23239..23400
                     /locus_tag="PLL59_01910"
     CDS             23239..23400
                     /locus_tag="PLL59_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22586.1"
                     /translation="MEQKNTKPALLSSLIGTPEIKVSLSNKTIALFFGALVASLIIIF
                     LAHAIIKKQ"
     gene            23486..23947
                     /locus_tag="PLL59_01915"
     CDS             23486..23947
                     /locus_tag="PLL59_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HMY22587.1"
                     /translation="MSVENNNVQAFLKMIRYAEGTAGENGYRTLFGGKLFNDFSKHPN
                     IKVPFFNKLKGKTDYSTAAGAYQFIRPTWLEVSTKLGLSDFSPASQDKAAVEKIRQRG
                     GLDYVIAGEFDKAVDKVKLEWASMPGAGYKQPEKKLAELKKIYVQHGGKFA"
     gene            23928..24188
                     /locus_tag="PLL59_01920"
     CDS             23928..24188
                     /locus_tag="PLL59_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22588.1"
                     /translation="MVENSHSNTVIQHLTDAFTDSVNVKNMLAASLLLMSKSMETLPD
                     LSIICKNITTVSTSIGAVLLLAYNGVKFYKELKNTNNDNSKK"
     gene            24169..24624
                     /locus_tag="PLL59_01925"
     CDS             24169..24624
                     /locus_tag="PLL59_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22589.1"
                     /translation="MITVKNRILDSATRRPMNNVNVFISDSNGNEVSRNGLPNIGRAS
                     DKNGQVIIPVANESDFVTYSFVGYQKLTIPGVTAQKKSNIIMRTVAQDLPETEIKYVP
                     RYEPTADKPTISNNNYKAGNNKKSWLIIGGVVGGLAIVGLLFYAIKNKK"
     gene            24621..24971
                     /locus_tag="PLL59_01930"
     CDS             24621..24971
                     /locus_tag="PLL59_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22590.1"
                     /translation="MKKYSAIIFITLFIVLMVAGLMIYRKKLLNDRLEKIVGFDFFAG
                     TQFKKGQPVRLNQNSVLNQVFESDGEMFVSGKITVKTSDKLTYVGKYNSLNVVKKKGW
                     FFDRYFLVTNDLII"
     gene            24968..25321
                     /locus_tag="PLL59_01935"
     CDS             24968..25321
                     /locus_tag="PLL59_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22591.1"
                     /translation="MKILKFLAITVGIVFLLGIILKWLEKSTKTTIKILSIDKINKTM
                     QVQVMANDSQTALHIFTFSEMFRKIYKANDYNLEVSAAGDIVFLRVLNEQGKEMALRV
                     INIKGTTAPIIIYSE"
     gene            25334..25576
                     /locus_tag="PLL59_01940"
     CDS             25334..25576
                     /locus_tag="PLL59_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22592.1"
                     /translation="MITQLSLFDKPKRKRKKAKKSIKKISNRGLLRSTITAQKKTISK
                     LEKQVKAEKKKETALKKKLTKVRRTAKPTTSRKRKK"
     gene            25595..25972
                     /locus_tag="PLL59_01945"
     CDS             25595..25972
                     /locus_tag="PLL59_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22593.1"
                     /translation="MKANHIARNASIKFLGNKYELFFQAINCDHIADNYRGFFSSHDV
                     KFVEIVNLLKGDIWVQNTKQTNKYKIISKYKGKYYRTIVTLSERIDLNKAFIVVISCY
                     ICNDKTEQNAYETYTIENKKRYR"
     gene            complement(26171..27268)
                     /locus_tag="PLL59_01950"
     CDS             complement(26171..27268)
                     /locus_tag="PLL59_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMY22594.1"
                     /translation="MDKTIEKKFFLQNKGYTLYSNNGCTSKRWYFLYYLNQAGVLFPY
                     KLYGGINRYSNADERFYAAKRLLNTLDVSNPKIEFDIEDLQNIFDVALKKRIPYLRKK
                     SIATYQSKINDFLDFCRRNRIDKLRKIDKKIATKYLQELLQKNNTTVNSYIITFKRLF
                     KDFVKENKIRFNPFEEIKKLPENRKGKMYFNTNQIEVLKSEIIKQNRILWFACQLQYY
                     CFIRPNEIRFLKIEHINTVDGTIKIDASFSKNKKTQVVVIPNCFLSDFIALSNKNEND
                     YLIQINNEQLKRDYLSKEHKKILAKLGFSNRYSFYSWKHTGAYMAVKGGIKLKDLQLQ
                     LRHHSLDQVNEYLREMGVLDCDDLKNKYPAL"
     gene            complement(27409..27495)
                     /locus_tag="PLL59_01955"
     tRNA            complement(27409..27495)
                     /locus_tag="PLL59_01955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27459..27461),aa:Ser,seq:gga)
     gene            complement(27590..28738)
                     /locus_tag="PLL59_01960"
     CDS             complement(27590..28738)
                     /locus_tag="PLL59_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMY22595.1"
                     /translation="MNFQLTEEHQQIREMARNFTQNVLKPGVIERDRDMIHPTEFLKQ
                     MGELGFMGMMVDTKYGGGGMDTLSYCIAMEEISKLDASCSVAMSVNNSLVCWGLEAYG
                     TEEQKQKYLAPLAKGEKLGAFALSEPEAGSDATSQKTTAEDKGDYYLLNGTKNWITNG
                     GKADTYLVIAQTHPEKGHRGINVLIVEKGMPGFEIGAKEDKLGIRASDTHSLLFTDVK
                     VPKENRIGEDGFGFKFAMKTLEGGRIGIAAQALGIAAGAYELAVEYSKVRKAFGTEIS
                     NHQIIQFKLADMATQIEAARLLVYKAAWLKDQHQDYAIASATAKLYASEVAMRVTTEA
                     VQVHGGYGYVKEYHVERLMRDAKITQIYEGTSEVQRIVISRGVLSGKI"
     gene            complement(28807..29262)
                     /locus_tag="PLL59_01965"
     CDS             complement(28807..29262)
                     /locus_tag="PLL59_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HMY22596.1"
                     /translation="MLNSKKHITDVQQLLSYLDRENKKIVFTNGCFDLLHQGHLHLLK
                     EAKKLGDKLIVAINNDASVRKLKGETRPIETLEIRIEKLIALDDVDYVISFSDDTPLL
                     LIEQIQPDVLVKGGDYKIDEIIGRQFAKNITIIPLLSGFSTTKIIGERK"
     gene            complement(29249..30265)
                     /locus_tag="PLL59_01970"
     CDS             complement(29249..30265)
                     /locus_tag="PLL59_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMY22597.1"
                     /translation="MKKHIGNLLKFIISLGIGVGLVFWSLKDVSTAQKQMIVDTFKKA
                     DYFWLLLLMIFGLGSNFFRAQRWRLLLKPIGYSPNYWNTFSAVMAMFFFNLLFPRLGE
                     IMRCTILYKYEKVPVEKSIGTMVLERVIDLFCIMILGVLILILEYQRFYSFFAEIAAK
                     INFIKLGIAGGIAIIIGGIVLYFLNKKKAVHDEIVREEHVIIAKLKGLWQGLISIKDL
                     EEKWEFIFHTFAIWICYILMPYFGFRCMEETAHLGFTAAMASVFFGGFAMVLTQGGVG
                     AFQIVIQKILVSYGIGEIIALSYGWISWSVQTFFVIIGGILSLIFLAIYNKSKKQTTE
                     HAQQ"
     assembly_gap    30401..30500
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30501..30802)
                     /locus_tag="PLL59_01975"
     CDS             complement(<30501..30802)
                     /locus_tag="PLL59_01975"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HMY22598.1"
                     /translation="MLVHVFRAKLHRVVVTEANLNYMGSITIDENLLDASGILPHEKV
                     QIVNINNGERFETYVITGERGSGQICLNGPAARKVQLGDKLIVIAYGMLPLEEAKS"
     gene            complement(30876..31727)
                     /gene="panC"
                     /locus_tag="PLL59_01980"
     CDS             complement(30876..31727)
                     /gene="panC"
                     /locus_tag="PLL59_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HMY22599.1"
                     /translation="MRIFQTVAEIQKYLLEINELRHTFVGFVPTMGALHDGHLSLIAA
                     SKTKTAITVCSIFVNPTQFNDKQDFDKYPIQIDRDLELLLSAGCDVVFVPTVEEIYPN
                     GTLEKTGIDLGFIGKTLDAAHRPGHFEGVLQVVKRLLDIVQPDILFIGQKDYQQCMVL
                     TKLVEHYQLPVQIEICPTLREQDGLAMSSRNARLNAEERIAAVQLSKALFTIKEHIHH
                     ADLLALIKEQLETLHQNRLISVEYIVAVNGKTLQTIDSYQENTPITLLIAAKVGDVRL
                     IDNVIVS"
     gene            31828..32637
                     /locus_tag="PLL59_01985"
     CDS             31828..32637
                     /locus_tag="PLL59_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HMY22600.1"
                     /translation="MEKKKLLYVTQEMDPYLVLTDAAHVVKKLAPYINDNGLEVRVLM
                     PRFGIINERRNRLHEVVRLSGINIIIDNDDYPLILKVASLPGARIQVYFLDNEEFFKR
                     KTVFNDEDEKFFDDNAERMVFFCKAALETVKKFGWHPNIIHCHGWMTSLIPVYMKTQY
                     AKDPVFSTTKMVYSVYEKTFSEKLHKNFLKLALIGDDIKDKDLAAFKDLTNEALTLGA
                     VQFADAIIQGSESIKELAAIKKMAGKKPFLTYQEMDNILPNYNEFYQSLLQ"
     gene            32656..34050
                     /locus_tag="PLL59_01990"
     CDS             32656..34050
                     /locus_tag="PLL59_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HMY22601.1"
                     /translation="MKKCSFLTIITLLVFISSCKKDTNIGADILPPDDLLNVKFTDTL
                     TLYSKTLADTFLRTDKLAKNYLGVINDIKFGTQKASIVMELDKPTSVYDDTLNTSYTI
                     DSVVLFLKYSSIYGDTTEPQSFDVSTIGNKINENSAYYYNTTQFPSTGLIGSVSNYLF
                     TPTTNKVHTTISDTVGVAGILRIKLDNSVGNTIINLGQSVLRDSAAFKNAFPGIRIEN
                     SNNLGRAMAEIDLNSLYSSIAIFYKDKYGTKKDMRLFTSIVKYTNGVAGTRVNGINLF
                     SNTLSSALQNTIASGELSDSISYVLGQGGATIKISMPTISNLGNIAVNKAVVAMAQII
                     PNASNLNTPYYLVLLKHNNDGHLDVLPTGDGVGILDTTTTDGFGNKIARYNFNITKYV
                     QAVANGTISNTDLYLGTYRFAGTDGTVNVLNSIVNGTVLSIGYSPARVIVAGANHSDA
                     RYKMKINLTYTKIN"
     gene            34118..35965
                     /gene="glmS"
                     /locus_tag="PLL59_01995"
     CDS             34118..35965
                     /gene="glmS"
                     /locus_tag="PLL59_01995"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HMY22602.1"
                     /translation="MCGIVGYIGKKQAFPIIINGLKRLEYRGYDSAGIALLSDQNALN
                     IYKKKGKVANLEEVAEANDVSGNIGIGHTRWATHGEANDVNAHPHFNATEDLAVIHNG
                     IIENYYSLKTELQSRGYVFRSETDTEVLVHLIEEIKKNVGCPLEEAVRLALNEVIGAY
                     AIVVISKKENNKLITAKLSSPMVIGFGENELFVASDGSPLIEHTKKVSYLEDGQMAVL
                     SIDGTVEVKSIKDNTSYVPYIDELQLKLEQIEKGGFDHFMMKEIHEQPQAIKNSIRGR
                     IDSQQFWVNLGGVREHELRFKNASKITIVACGTSWHSALIGEYLIEELARINVEVEYA
                     SEFRYRNPIITENDVVIAISQSGETADTRAALELAKAKGALTYGICNVVGSSISRMTD
                     AGSYTHAGPEIGVASTKAFTTQVSILALLALQLAELKGTLSQTKFHEYITELEALPSK
                     IESILKAEPYIKKIADTFQNATNFLYLGRGFNFPVALEGALKLKEISYIHAEGYPAAE
                     MKHGPIALIDENMPVVVLAPHTQHYEKVLSNIEEVKTRKGQLIAVVTEGDAEISKIAE
                     YVIQIPDTPEPFTPILSVIPLQLLAYHIAVMRGCDVDQPRNLAKSVTVE"
     gene            35965..36444
                     /locus_tag="PLL59_02000"
     CDS             35965..36444
                     /locus_tag="PLL59_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22603.1"
                     /translation="MQIIKTLSFLFFLYISFVSHAQTIPYNVQKKKYPLGEQFQNLLP
                     LKLEKWNRYAYHDFVPNHETGYVYYRKDNQQVYLTFGKAYSQSGLNAEWSKIYDDAKE
                     SKPGKLVHHNPATTATKYLYVNGNKTCFFAWTRNFYYFTVEAKSKTLVDEFMKVFPY"
     gene            36508..38319
                     /locus_tag="PLL59_02005"
     CDS             36508..38319
                     /locus_tag="PLL59_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMY22604.1"
                     /translation="MQTNSSVFLGDKFYNREHLNFILFDVLKAERVTAYEYFSDHDKE
                     TVQLYLDASDQFAKEYLFPHVVEMDRVQPELVDGRIRVHRAHKDIMRISGENGWISIL
                     ASKEVNGMQMPSVFSTAASFILGAANYSGCVFPGLTMGAAHLIETFADKKYHAIYLPK
                     MFAGEWQGTMALTEPNAGSSLSDIVSSAEKQADGTYKIVGQKIFISCGDSDAVENVVH
                     LLLARIKGAPTGTKGISMFIVPRERINENGALEYNDVTSIGVYHKMGYKGAPIAHLSF
                     GENNDCKAWLVGEENKGLTYMFQMMNEARISVGLHATSIASAAYYASLKYANERLQGR
                     PITEKNPELPQVPIITHADVKRMLLFQKTFIDGALCLELQCSYFADMERVTEGDEKEK
                     YNLLLELLTPVAKSYPAEMSNFSTSTALQIFGGYGFTKDFIAEQYYRETRIHAIHEGT
                     TAIHGLDLLGRKVMMKNGKAVMYLTQEVMQAIAKAKKHENSKEQAENLEKQLAKLQEL
                     SMHLMQVAQQKGVEAYLSDATLYLELFGILVLAWQWIKMANTCNENESIDKHFKESKF
                     LCFNYFFEYELPKVQGMFTRLKSTNHITVGIDSSLID"
     gene            38419..38784
                     /locus_tag="PLL59_02010"
     CDS             38419..38784
                     /locus_tag="PLL59_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMY22605.1"
                     /translation="MTTIQQISLYIMAAFYAGGGINHFVNPKFYLGITPKFFPIPNVL
                     NYVSGVAEIVLAIGLLFASTRQISAYLIIAMLILFFAVHISHLFHPPKMAQGKEWFLY
                     VRLVLQFALIYWAWRVGKY"
     gene            complement(38754..39266)
                     /locus_tag="PLL59_02015"
     CDS             complement(38754..39266)
                     /locus_tag="PLL59_02015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY22606.1"
                     /translation="MKIRIAFVNDIPKIIEVAAATWKQTYAPILSQEQIDFMYERMYT
                     SEAILQQMQNGNTFLICSENEKILGFISYILREDNSICIPKIYVKPDTHGKGIGKLLL
                     QEVEKIAQKNKLDFIELNVNRKNPAMYFYKKMGFELHEKVDIAIGKYWMNDYVLRKQI
                     SQYLPTRQAQ"
     gene            39342..39584
                     /locus_tag="PLL59_02020"
     CDS             39342..39584
                     /locus_tag="PLL59_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22607.1"
                     /translation="MSATEILKTKLHTFIENISDEKILNAHAVLLQNEIEQQTEDWNT
                     LPAELKEALQQSISEFDNGNYEPIEDVLTKLKKNGI"
     gene            39574..39864
                     /locus_tag="PLL59_02025"
     CDS             39574..39864
                     /locus_tag="PLL59_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMY22608.1"
                     /translation="MAFKIIISKSATQSLQDILDYTSLNFGKKKHDILLKKIEDAIFT
                     ISEHPLLFPKIKETIHRVLIINEISMFYQIKSTSIRILLFWNNRQNPNRLRL"
BASE COUNT        13701 a         6533 c         6838 g        12803 t
ORIGIN      
        1 gctccgagtg ccgagttgcg cgatggacaa aaagattcgg attctttgcc tgattatgct
       61 atattggaca aagtgttatt tcaatacatt gagcagcatc aaggaccaga tgagctggaa
      121 gcaatgggtt ttgatgaaaa gttgataaca agaattttaa gaatggtcaa ccgtaacgaa
      181 tttaaacgac atcaaacgcc accgatatta cgcgtatcgc caaaagcatt cggctctggc
      241 agaagattgc caattgtcgg aaaatattta gaaagctaat cgcatgcata tccatctcat
      301 taaaaaacaa acaatcaact atccatctgg ttcagcgatg gaattttata ataatatact
      361 ctatatcatc ggcgatgacg tcaattatat cttgtgttta gatgaagcgt ggaaggaaat
      421 aaaacgcatt gaattgtttg cttttacagg cacacgcatt ccgaagccag aaaaaccaga
      481 ccttgaatgt gctacaatta ttggcaataa gttatatgtc gtaggttctg gctcactttc
      541 accacagcgc gatgttgctt ttatagtcta cctcgaagag gataaagtaa aacgaatcag
      601 cacggcagca ttttatgcac tttttcgcga tagaaattta gtggcagaaa tgaatatcga
      661 aggttttaca gactgcaaag ataaacttct gttttttaac agagccaaca ctcaacaagc
      721 caaccagctt ttaattacag atcataaaat catcaagaaa caatttcctg ataaattcaa
      781 agtgctgcct gttgaaatag caaatcttcg acaacttcct ttaggtattt ctggtgcttg
      841 ctatgatgat aaaaatgata ttttattcct atctgcatct gccgaaaata cgagcaatgc
      901 ttatgatgat ggcgaaattg aaggaagcgc cataggtgtt gtttttaatg cctatcagaa
      961 attaaatgac aaaaaatata ctatcgacaa actgatttgt ttagacgatg tgcatcaaga
     1021 ttttcagcaa caaaaaatag agtctatttg tatattacgt caatccaata atacatatcg
     1081 aatggcaatg gtggcggata atgacgatgg gaaatcggta ttatttgagg tggaaattac
     1141 actataaaaa agaccttcat tacgaaggtc ttttctagcg taagattata tttgctactt
     1201 atcttccaat gctttattga tgacaaatgc caagtgtgct cgatgtgctt ttgttgccgc
     1261 atcgccgccg ctgtaagcat tcatccaatc caaaatgcgt tttacttcgc caaacgcagc
     1321 agatttagac gtataggcat atgctgattt tgtatcgact atcgataata atctgcttac
     1381 atattcttct tgtaaatttt gtcgaatgct agaaacagaa gcacctaaat cttcgatgaa
     1441 aataccattg gtgatatctt ttaacacagc atttagtgtg tatttattgc catataattt
     1501 agtatctgtt atacgttgta atacagtgct attgagcaat tggtctaata tgccacgttg
     1561 tatatttagt atgcgttcgt gtatttttgg atcttctgtt tccttgcgaa aatcttgtcc
     1621 tcttctttgc atttgcaaga aattatataa ttctgttggt gcagtaaatg cattttttga
     1681 aaaggcatat ttagtaattg cctgcattgc atttttttgt tcgcttaatg gtatgggtgt
     1741 aaatggcttt tcttttgcat cttgcccaac aaaatttcta ttgacatata caccaccaat
     1801 gtatctgctg atgacattta aactacgtgc ataatgacca gacaatgata aatatgcttt
     1861 tcgcaattca tgatatgaat tgcccgtagt actatatttt gttagtagtt ttggcatggc
     1921 atcattcacc atttttatac tgctaacagc ataagcaact gcatcactag acatatcaaa
     1981 tagattgccg cgcgggtcta tgccttttcc cgttgagcgc atatcgtcag catcgttgcg
     2041 gaagcgatat gcacgtttgt cagactcgga taatattttt ttcaatgctg tattttcttc
     2101 gtcttcattg gcaatatttg tatatccata ttgaattgcc catttgtcat acaagcctgg
     2161 tttaacatca aaaaacaagc cttgtttgtt tttgtctggc gaaatattag gaatggtgta
     2221 gtccattaca gatgctgtaa gtccaacagt agcacctaaa acaggatctt gcatttcatc
     2281 attagtatgt aagtttgatg cgatgaagtt gtgcattaat ccgaaagtat gtcctatttc
     2341 gtgcaatact aaatcatata atgcttgttt caaaaactca tctttatcga tgtctgaaaa
     2401 accatttgct gctaacagtt gtgtgccata taatacgttt tgatgcattg tttcaccaaa
     2461 agcacagttg ctatgttgca gtggattttc tgattgcatg tggtctaatg cagcgatgtc
     2521 atatagtttt tcgagtggca aacgattggt catgtaaatg aattccaaca tgatatctgc
     2581 gcctaagatt tcaccagttc ttggattgac aaagctcggt ccgtagccac caaatggtgg
     2641 tcgtggcgaa gatgtccaac gcaacacatt gtatctaatg tcttctgctt cccagctggc
     2701 agtatcaggc tgctcgtagc acgcaactgc atttttaaaa cctgcttgct cgaatgcttc
     2761 attccaagcc aaaacggcat tttttataat tggtttaaat tcggcaggtg ttgttttttc
     2821 tatccaccat acaattggtt ttacaggttc tgacagtttt tcttcaggat ttttcttttg
     2881 taaattccat ttgtgaatta agtctttcca cggcgttgca ctcgcagagg tttggtcgtc
     2941 cactttttct aagaaatagc ctacacgtgg atcatctgct cttggtctaa aatcattatt
     3001 gggaacttct attaagttat gttgcatttt tacggaaacg aaacgagcgt ctgttacttc
     3061 attgctacca tagtttgcag gcgctaaatt gtcaaataca tattctacgg aaacggcagt
     3121 gttgtcaggg taatttttta ctgctaaata ttttgatttt ttgctattca gtgtgcctaa
     3181 tgaaaaagca tctggtcttt cgccaggcat ttttgatggc ttaatttgag taacactttc
     3241 acttaggaat aagttgtctg catcaattaa ataagttttt ccagttgctg caacaatttt
     3301 ctcagagagg aataaggatt gattaatgtt cgcatctttt gctttactca agttgttttt
     3361 ctcatcgaaa taatagtttg tattttgtaa gacaaagtct attctgtcga aatatttttc
     3421 tactttaaaa atggaattgt ctttatagcc accacgtgcg aagccaacat ctaaataacc
     3481 atctaacaca taagcgaaat aaatgtattc tttgccgatt ttctcttcgc taatttcaag
     3541 atacgttttg ccgttggttg tgtcttgata aataggaaac aaacctgcag tttttctgca
     3601 tttttctgta acttctttaa tagccttaaa ttgaggcttg ctgtcttttt ttgaagtgct
     3661 gtcttttgct gttgttttta aagaatctgt catggttttg acagtatctt tgtcagtttt
     3721 tttcttttct ttctttgcaa agatattcat gctcaaaagc aaaaaaatac ttgctaaaat
     3781 ggtgatgcgt tgaaatagct tcatgttaat ttttttacgc tgtgaaaata ctgtttttta
     3841 tgcttttaat aataaaaaag ccactctgat tagtagagtg gctagatgaa atattacaaa
     3901 agcgatgctt atttgtcata ctgacagcat ttcggaagct ttttataggc ttcttctgtt
     3961 gctttgaatt tctcgttgtc gtatcctgca ttggcaacat tctgttggat tttctccaag
     4021 tttgtttttt ccactgcaaa tgacactgtt agtatgtgcg tatcttcgct ccagtcagca
     4081 tgctttacgc cagccgattt tgctgctttt tcaatgcgct ctttacacat gtcacagttg
     4141 ccagatacct taaatgatgc ttttgtcaat gtgtcttttg ccatcatatt tcctgaaaag
     4201 aataagataa tggcacttgc tatactaaga atttttgttt tcatgatttg taattttaat
     4261 gtttagtaga atcttcttta cagcattttt ctttttttcc acttgctgct gtggagtctt
     4321 tgctacagtc tttttcgtct tttttgcagc atgctttttc ttcgccagct tggcatttgt
     4381 cgcagcattt tttgtcggca gtacagtcgt tgctacattt atcacaacat ttgtcgccat
     4441 ctttgcaaca gctcattttt tgttcacatt tttcaccgtc tttacagttg tttttacatg
     4501 cagtaaatcc gaagatggca attgctaata ccatcactaa agccaagatt gttgttgttt
     4561 gtttcatttt gtttgttttt aatttgttta tttaataaat aatctaaatc cgccatatac
     4621 atttgcaccc ataataggag catatacttg ataagtatca aaaaccgtgc taaacggtgt
     4681 acctaaaatt acgttttttt gtctgtagtt tgtgatgttt tcacagccta taaaaatctc
     4741 gccatgtttg aatattttca ttatttgtgc attgagcaat gcataaatag gagagaatcg
     4801 tttgcctgca tcgccttcaa aactattagg caatctgcgt ttgccattga tgctggtgtt
     4861 gatgtcaaac tgccactgtt cattaggagt tttgtagctt aatgctatta aacctttgtt
     4921 taatgcctgc aaaggttttt gtaacaattt tccatgataa gtagcacgga catcttctaa
     4981 acgataggcc atttttacat caagaccttt tgccacttcc atattgagct caagtaataa
     5041 gctattagaa aatgatttgc ctttcaagtt tgaaatagtg gccaagccat cagcgttatc
     5101 gatgtcaacg atagcttgtt ttgtaaaatc tgttctgaaa aaatcgactg taaaattagc
     5161 ttctctattt ttcaaaaaga atttttgtgt caatgatatc ccgtaattcc aagcgatttc
     5221 ttttgatgga aaatcttgga aaacaatagt tcgcgagctg gctaacaaag actgattttc
     5281 cgacagaata ttaggcacac gaaaaccact tccagctgca acacgcaatg tagtgttgtt
     5341 ggtcaaggag aatttagcat gaattcttgg attaacctga aatccaccac gcgtgtgata
     5401 atcgcctcta agacctgtaa ctagcgttac tctgttagtc catttgtaag tgtattctgc
     5461 aaaaattccc ggcacaaaat catttctgtt gaattgtatc gtgtcgagtt tttctgtgat
     5521 tttatcatag ataaaattag cacctgcaac aaaagtatgg ttttcatttt tgatatctgt
     5581 ttgatataaa actgtcccag tgaaactggc ttgtttggta ttaaacaatt gttgaccata
     5641 attggagttc tgttgatgat acacacctga aaactgagtg ccaatgctat tgttttttga
     5701 agtattgaat atataaccag tctttgcaaa agcttccact ctttttgttt taataccaat
     5761 tccaaataaa ggtaaagtat cattttcgtg atgagcagta atttgtcctg ccgttctgtt
     5821 ttcgcttaat acttttacca tgtattgtcc ttcaatattt tcgccatgat aattccatct
     5881 attgagtaaa ttatattgct tcatcaatgg ctgatctaag aaaccatcgt ggttcatatc
     5941 ttgttttaca ggaacaaatg caccatttgc cattaacata gtacctaatt ttttattgat
     6001 tttatggtta atcaataagt tagtttcgat acgtgcattt tgattgccaa acatatcaaa
     6061 gaacaatttt gtgctatcat taaagctgtt tttaaactcg acattcatag agccagtaat
     6121 accttcataa ctgtttttta cggagggcac acctttggaa acagaaattg attttatcca
     6181 aggtgctgga atattatcta atccgaaagt cgccgtaact cctcgcatga caggaattcc
     6241 ttctagtaaa ttctgcacat aagtgccatt caaacctaaa agttttatct ctttagcacc
     6301 agtcacagca tcactgtaat ttacatctac tgaggcattg gtttggaagc tttccgaaag
     6361 attacagcag gcagctttct tgagctcgcc ggcgtctatc atttctgttt ttaatggatt
     6421 gatagaggaa ataaaatttg tttgccgctt gccttttatt tttacttctt ctaaagcgat
     6481 agctggatcc aatgtaatat tcatttctgt ttgatgatgc acttctatcg tgtcatcttg
     6541 atagccgata taactgacga ttattttctt tggcaaagcg ccagctacgt ttaattcggc
     6601 aatgccatat tcgtcggtgg tggcaccaat tgtagtatct aaccactgaa catttgcgcc
     6661 atacaacatt tcgcctgttg ttctgtcgct gacctttatc ttaattttct gtttttcttg
     6721 agcaaagcct gtatattgaa atgctacaaa tacaaatgca aaaacccaat tattgattat
     6781 tttcattgtt tgttattttg atgaataaaa ttgtactaca caattttatt atcgctaaca
     6841 acgaaatact tgaagcttaa cactcgatgg tgctaaatga gattttataa tagtagtata
     6901 taaattgtct ggaggcggta agcctgtatg aagttgtgca atatatgaag gcaatatgtt
     6961 tattgccaaa gcattgaaaa aaggaattag gaaatcttca acactattta ttttcaattg
     7021 ttttgctgta aaaccttcta ttgataattt agaaaagaat tgagagtgcg aacaacaatc
     7081 agatgattct tcaatagcag gtttgttaca acaagacgta gtttttactt ctttttcaca
     7141 attaatttct gagaaaaaag aatagtcttt tgatttcgaa gagtcgcaaa tatgctcaaa
     7201 caaagcaata ccattactag atacaagtac cagtaatgct aacaataagg cagatatatt
     7261 tattctcttg ttcatctatt tgcaacacaa aattactaca aatattgata taaagctgaa
     7321 ataaattgtt tatacttagt taaaaataat tatcaattgt tagtgttgac ttaatataaa
     7381 aatcattaag tttgaataat atgacattta aagagattat tcagcaaatt accaatccta
     7441 aagaggttac tcagtcacat gagtttatta gtaagtttcc gaataaagtt ggtagctacg
     7501 gttatgacgc ttggggcttt aatattaatg gtattaagcc atttgtaggc atgggtcgat
     7561 ttttttacga aaattttttt agagtagaag tgcatggctt agaaaacata cctaaagaag
     7621 gaagatgttt gattattgca aaccacagcg gacagcttcc gatagatggc atgatgttag
     7681 gatatgctgt tgttaccaat ccacatgcac cacgagcacc caaaggtatg tatgagcgct
     7741 ttgttccgca agtgcctttt ataagttcaa tattttcaca gtggggcggc acggttggtg
     7801 atccagaaaa ttgtattaaa atgttggaaa acgatgaagc agttattgtt tttcctgaag
     7861 gcgctagagg tatttctaaa tcctataaaa agaaatatca attacagcgc tttggattag
     7921 gatttatgta tatggcaata cagactaaat cgccaataat tccagtaggc atggtcggtt
     7981 ttgaggagtc gatgattaac tttgggaata tagacttttt agaacgtttt ctgaagttcc
     8041 cagcagcgcc agccttagtg ccatttgtgt ttccatcaaa agtcattatc aatattggaa
     8101 aaccgatgac atttgagggt gatatttcaa gagaatatca agtgaatgag aaagtagatg
     8161 aagtgaaaaa agaaattaat agactgatag atgtaggctt acaacaacga aaaggaatat
     8221 ttagtaaata attgtttcaa aaaatcaaag agatatacag acttgcttgt atatctcttc
     8281 ttatttaaaa cgcgattgca ttatttactt tcttcttatc taatgcaagc aataacacct
     8341 cttccatagt ttctacataa tgaaatttta agccttttat aaaatcagat tgtacttcgt
     8401 caacatcttt ttcattgtct ttgcataaga tgatttcttt tataccagca cgttttgctg
     8461 ccaatatttt ttcttttatt ccaccaacag gcagtacttt tcctcgcaat gttatctcgc
     8521 cgcttaatgc agtatatgct ttaatttttc tttgtgtaaa taaagaagtt aaggccgtta
     8581 agattgcaat acctgcactt ggaccatctt taggaatagc gccttctggc acgtgaatat
     8641 gtaaattcca atcggtaaat gcttgagcag gaatttttaa atcatcagct tttgatttga
     8701 tataagtaag tgccgtgttg aatgattctt tcatgacatt acctaaattt ccagttatca
     8761 tcaggttgcc tttcccttta tttacagaac tttcgataaa taatatatcg ccgccaaatg
     8821 gtgtccatgc caatccaatg gcaacgccac aaggattatt ttctgaatat ttatcgtgat
     8881 tgtacttcgg ttttccaaga atgcgtgtga tgtcatcagt agagatgcta acattgtatt
     8941 cctcttctgt ggcaacgctt tttgctacgt tgcgcatgat agaagccatc actctgtcca
     9001 attctcgaac accgctttcg tatgtgtaat tgtgaattaa atattgaata gctttgtcgt
     9061 tcagtttaac ttgatttgac ttaaggccat gcgcttcacg ctgcttagct actaagtgtt
     9121 tttttgctat ttctgttttt tcttctaagg aataaccact aagttgtatt atctccatac
     9181 ggtcgcgcaa tgcaggttgt atttcgctga tgctattagc agtagcaata aataatactt
     9241 ttgataagtc gtattctaat tctaaataat tatcgtagaa agttgtgttt tgctctgggt
     9301 ctaatacttc taacaaagca gaagaaggat cgccacgatg gtcttttcct actttgtcaa
     9361 tttcatctaa aatgaaaaca ggatttgaag attgtacttt cttcaaagat tgaatgattc
     9421 gaccaggcat agcaccgata tacgttttgc gatgaccgcg aatttcgctt tcgtcgtgaa
     9481 gaccacctaa agacatacgc acatatttcc tgtcgagtgc acgcgcaatg cttttaccta
     9541 aggacgtttt tccaacgcct ggaggaccta taaagcataa gatgggcgat ttcatatcgc
     9601 cttttaattt taatactgct aagtattcta aaattctgtc tttaatttta tctagtccgt
     9661 aatggtcttc atctaatatt tttttaccgc gttttaaatc gaaattatct tgcgtagttt
     9721 cattccacgg caattcaacc atcaaatcta aataattcaa aataacggaa tattcagctg
     9781 ctgctggatt cattcttttt atacgttccg tttctttgtc aaaagctttt gcaacagcct
     9841 cactccattt tttctcttta gctttttcct tcaaacgatt caactcttct tgatacatat
     9901 cgccaccgag ttcttcttga atggcgcgca tctgttgatt taagaaatat tcacgttgtt
     9961 gtttgtcaat gtcggttctt gctttgttat gaatatttgc ttttaattct tgcaattgca
    10021 attcctgatg caaatatttt aatacttgtt gtacgcgctc gtgcgtatcg ttagtttcta
    10081 aaatttgttg tttttgtgcg agttcaatat tgagattaga agtgatgaaa ttggctaaga
    10141 attttatatt gttgatattg tttaaaacaa tttttgcttc attaggaatg tttggagaaa
    10201 gctcaatgat tttattggcc atgtccttaa ggttgtccat cattgctttt tcctgctgtt
    10261 tgtctgtcat tggaatgtcg gcaagatatt ctacttccga ttcaataatg ggctcagatg
    10321 taacattctg tttgacggca aatcgtctta taccttgcag gattaatgtg tttccgccat
    10381 ctggcatcga cagcattttg atgattcttg caattgtacc aatcgtatag agatcacttg
    10441 gttctggttc ttctactttg atatcacgtt gtgcgataac accaatatat ttattgcctt
    10501 tataggcttt ttttaccgat ttaatggatt tttctctacc tactgagata ggaatgacca
    10561 caccaggtaa taaaacattg ttgcgcaagg gtaatattgg caatactttt ggtagatttt
    10621 cctcattggt attgtcatct tcttcgattg acaacaatgg taccatctct acatcttctt
    10681 ctattatatt ttgcaattcg attttctgga acatagttaa atttaattca atttcgacaa
    10741 tatgtcatgc gccttattgt taataaggaa taatagctaa tgcaagatgt atgccgaatt
    10801 gcttaaagta gaaataaatg gaaataatgg cagattagga gcaataaaaa aacagtaaaa
    10861 tgctcggata cattattaag tatcattttt tttgcggaga cggcgagaga gattttaatt
    10921 actaattgtt tggttttgtg ctttttataa tttctcactt gttaatcagc tttttattat
    10981 ctaaaaatat tcggaaatcg tttttttaat acttctatca ttgcatcttc aaattcagat
    11041 gtattagttt ctttgttcaa tgagcttttt atttctttat gctctttgcg caattctttg
    11101 cttaattcag caaaaaaatc atactctaat gcaatagaaa tagattttat aatatgacta
    11161 tttaaatctt tatttttggt tagtagtcca ttaatgtact gtctgttttt tcccattttt
    11221 tcgcctaatg ccgtctggga aactcctttt ttttccaaat attctgatat tattttagta
    11281 atactcataa tcaaattttt acataagtag ttaaaaaaca tacacttgta gtttttttag
    11341 aattttgtag tcaattttaa tacactgtgt agtttatttt actacatttg tattgaaata
    11401 caattacact ttatgaactt aaatgcaaaa acaaagccaa aagcaaagca aaaaaagtat
    11461 gtagctatta ctgaatatcg ctacaatatc aaaagaagac tgtctaatta ttcagccaat
    11521 caaaaaagca gaataatgca ccacgtgcag caggaaacgc aaattggttt acgcactatc
    11581 tacaacgtaa taaacgagga aaaaggaaat tttaagcaca ataacatcaa tattttattg
    11641 gcgtttgcac gtgtttttaa ttgtgctatc gacgatttaa ttaacagata aatagaatag
    11701 catgcaaaac gattattata aaacaaaaga gcgaaaaacg ctgaatgtgt ttaaaatttt
    11761 ctacttcgct actattataa tactactcat tttatcatta atcataaaaa tttttgaggc
    11821 atgaaactaa acgaattttt agaaaaaaaa caaaagatgc accagctgaa tgaagcaatt
    11881 tgctacagaa aaaattttat cgaaaaaaat tacaacgtca ttaagttcag aaaaatgatg
    11941 ctgaattttc gccgcgaaat attggctttt gaaataaaag caaaacatct aaacgaagat
    12001 atagaagcgt atgaagatcg tttctgcagg ttgctggaca tacaagtatt gctaaacagt
    12061 ctaaatgttc aagaaaaatg aaagatagtt gcagtatgcc acatgttgtt agaagattat
    12121 ccgatgaaaa attattttat atgcgctgct gtgaagtatt taataaagat acaaatgaac
    12181 atgatattga atttcaagtc tttaaaaatg gaaaatggat aaatgatagt tcaatatgtt
    12241 cacctaatta taatttttat gttcctgata atgaaattaa aaatttacaa ctttttttat
    12301 tatgaaccca aactacaatc aaatagaatt taaagagtac gaacggcgta tgatggcagt
    12361 attggctaaa atgaaaatag acctggacga atacaaaaaa aaggaaaatg caaatgctgc
    12421 atacattcaa cgcaatgaaa atattttaaa acatctatta gaatatgttg ctgaaatagt
    12481 gccagaatat attgaaatat tagagcagca aaacaaaaac agttatcgtg ctggctacaa
    12541 tgctggcgca gctgatgcgg aaaaaaaata cacatggaaa cctgacatgc aagacaaaga
    12601 aaattttaga acctactgga ccttagagca aaaaaaaata ctaccaaacc tttattaata
    12661 ctatgagcaa aaagatagat ttagaagatt taaacaaatt ttctgataca gccgtaagtt
    12721 acgctgtaga aaaaccaaag ttaatacgca taaaagagca tattttttcg ctatatgatt
    12781 ttagatacaa caaagtgcgt ttgctattgg aatatcgcaa gaaaaatgag aaagactgga
    12841 tagactttga cgaagatttg attaatgata tttggataaa ttttgaatta aataacgaat
    12901 ttcattctaa agaaaaacct agcacacgtt acataaacac ggtgcttttt tctagttttg
    12961 ttgctaagta taatcctata cacgattatt tttctaattt aaaatatgat ggaaacagct
    13021 atataagtca gcttatacaa tgtgtagaaa ttgagccgct aagagtaaac gacgattata
    13081 cattagacat gttttttcct ttattgttca aacgctggct ggtggcttgt gtggcgtgtg
    13141 cgctaggtta caatgcaaac catgttttat ttttactcat tggtggacaa ggcacattta
    13201 aaactacatt tctaaagcac ctggcaccaa aagcaataga tgattattgc tttactggcc
    13261 agatagattt agacataaaa aacaaagata cactgaacta tttagccgaa aaatttatta
    13321 ttaatcttga tgatcagttg caaagcttgt acaaaaaaga ttttaatgtc ataaaaactt
    13381 tgattacagc atctgacatt actaacagga aggcatttag acgagacgac aaaaaacgta
    13441 aacgtatagc gaactggtgc ggatctattg acaaggaaaa aatattcctg gacgaaaaaa
    13501 acagacgtta tttatctttt aaaataaaaa agatagattt agccaaattc caaaaaataa
    13561 atattgataa tgtttgggcc gaagcatacg atttactaaa aaaaggcgaa cgctttttct
    13621 ttgatagcga ggaagtaaac tacattaatc aaataaatag actgtttagt attgaaacac
    13681 cagaggaaga atggctactg gcattgttta ataatgcaga taaaacggac agtccagcag
    13741 aagttgttta tttaatgttc aatgaaattt tatctattat gcagcgcgct agcggtttgc
    13801 gcatggaagc aaaactggta acaatagcca tgaaaaaact agactggagc gaaccaataa
    13861 gccagcgcat aggcattgac aaaacaccac gtaaagtata tgcagtagtg cgcaagtttt
    13921 acgaagaaca aggcaaaatt ataatgaaat agcaattaaa tgaatttttt agtaaatttg
    13981 atgtatgata atgcaaatat taaatattga caatcccaaa cctcgcaaat atgcctctaa
    14041 aaatgaagct ataaaagaaa tatccaaaga aataaaaaga agttgggcga aaaaaaattt
    14101 aaaaaaattt cttgatgata gtaatatcag acaccaatat aatagttagt gcattaatta
    14161 gcccaaatgg tgcagttgct aacatactta attcaaaaag aaaatttaaa ataattgctc
    14221 cagacttttt gttagatgag attgacaaac attttgacaa aatattaata aactccattt
    14281 taacagaaag aaaggagtta aaagcactca tatcctatta caaggaaaaa attacattta
    14341 ttccaataga cgaaatacca caaaaagaca gattaaatgc ttataaattg gtaaatgata
    14401 ttgatataga tgatttgcct tttgttgcac tttctattca caaaaaatgc ctcctttgga
    14461 cttccgatat aaagctcatt aatggactaa agaaaaaagg ttttactaaa gtaattacta
    14521 cttcggagct taaaaaattc atctacaaaa aataaatttg ctttttggca taaactttgc
    14581 tacttttgaa gtgcagttac aaatgtatga gaaatcaaac acgaaaattt attaacaata
    14641 agccaggcga gagttttaga aactcacctt ttgctcatgc atctgtgact gcagcgcctg
    14701 gcccttttaa tttttttaaa tatgcagtca cagaaaaaaa cggcaacgaa agcgcaaggc
    14761 gcaagtaatg ccacagaaca agaaagcaaa ccagcagcag caaaagccta caccattttt
    14821 gcagagccta ccgacgattt tattttcgac aaacttttcg acgaaattta ccagctgatc
    14881 caggagtgta taaaagaagc tacaccaatg aacgcgaatt ttataaacga aacacagcaa
    14941 aaacatgcta tatatagtat attgtatgtt tgcttagaaa aacaatatcc ttacaaaaca
    15001 gttttacaaa atttgcaatg cttttttcat aatatcgatc ctgaatacat tatgcaagca
    15061 tgtactaatg tgtattccaa tcggtttttg acggcctaaa aacaccaaaa aacaccaaaa
    15121 acgctccatt tcggagcgtt tttttatttt gcttactaca aaagtagttt tgcttactac
    15181 aaaaaaaatg agttttttaa caaaaaacag cctaaaaatg ccatttttta gctaaagcga
    15241 caaaatttag ttttttgctt actacactta ctacaatata attaattgat tatcaatata
    15301 aagttgtagt ttatttgtag taagtgtagt aagtgtagta atgtagtaat gtagtttgca
    15361 aattttaaag ttttgcgccg tgaatttttt tcattttcta aatgattttc catgaaaaaa
    15421 tactttgcat ctgaattaca cttttaaaaa tgatattggt ttactattta tgtttgtaaa
    15481 taaaatagta acaatgacta cagaaaatga aattttaaac gatttaccag agacagaaac
    15541 tactgtttct gaagtagaaa ccaatactgc aaactggata aaacctaaaa tgacattatc
    15601 attttccgaa aaaaacgcta cacgtgtaca atcgtttgta aatggtttgc aagatagcaa
    15661 gcgagtaaac aacattagca aactgtttat gttgctattg gaaacagcgg aaaaacaagc
    15721 ggaaaccgaa agtgtacagt ttacaaaaat ggaaaaacaa gcagaaacca ttagtaaaca
    15781 agcagaaacc atagcgcaat tagaagcgca gctaaaacat gccgaagcaa accgtaaaca
    15841 aacggaatac cgcacggaaa ccaaaggcgc accagccgta aaccaacaaa aaacgaataa
    15901 taaaccagca gaaaccacaa aaagtggcta tgcaaacttt tggcaataat ggaaaataaa
    15961 ctggaaacta gtgaaaaaag agattaaaaa tttagtacaa aaggcattaa cgggaagcaa
    16021 taagcatatg gaattaacta ttaattattt gccaaagtat caagcacaaa ttatcgaaaa
    16081 aattattggt atagatttga atggagtatc acgaagtatt gatacttatg gaataaaaca
    16141 tactattaaa gaacatggaa gtgagcgtac agaattagct agaggtcaat taccagtaaa
    16201 tattgatgat tttgaattaa taccagaaat attaaaaaca gacatagtta aatatggtgg
    16261 taaaaactct ttgaaacaag atgtatttgt atttgaaagg agaattggca acttatattt
    16321 tgttgtggaa gccgttagat tttctaaaaa aggaaacaaa cttgtatttc aaacacttta
    16381 taagagaaaa taaaaggcgg cggttgagtg ccaaaggttt tatccttgcg tctttcaaac
    16441 gtccgaaacg ctccgcccta atacaaaatt aataaaaaaa tctaactttt ggcaataatg
    16501 gaaaataaat tggaaacaca acaagaaacc gtaacaatac cacgatatgt atatcgaaga
    16561 ttgttagatc agcgcagcga attacagcag actgtaattt tttcgactga tgttatttta
    16621 atatttgtcg atttattagg cggaaaatta ccacaatcga aaaaagacat cattctatta
    16681 ttgccaaaat tgccaaaatt aatagatagt ctttcagacg aaaaaaaacg cagctacttg
    16741 caagaagcgt ttaaaaacat tttggaaaca gcacctaaat acatcaccga aaaacaagta
    16801 gaaaaactaa aagcaatgga tctattgcca ttgcaagaaa atttataaaa tatggaaaat
    16861 acaccaattt taacagaaac agaaaccata attggaccag aactaaacga agatattttc
    16921 gacattgaaa cggaattatc aaagccaaca caacaccgtt catttgattt tgatgcagta
    16981 agcaacgaag cagaaaatat gatttctgaa acggaaactg tttttaatga tgacattgat
    17041 acggaaaaat taaaagaaac gctggaaaat aaagtagcgg acaattttga agccgtttac
    17101 gatagttcca cactggcacc agccattatt gacggtgcag atgctattat acagcaagta
    17161 tttcctatac tgtatgaaaa aacagccttt agcgaggacg aacgcaaact aataaaaata
    17221 ttagcacaca aacaaaaaaa caaaaaagaa accacgctaa cagatgaaga cttgtcctta
    17281 atgtcagcat gtgcagaata tgaaaactac attgaaagtt tgccatttac tgaaaatgaa
    17341 aaaaagcaaa ttttaaagcc actatcagaa cttttaaaag atgtgaattt gcaaaccacg
    17401 cctggcaatg ctttaattat tgccgtattg attattatga taccacgttt gatgcctata
    17461 ggaataaaca aattttttaa aaactaaaat aacaaaatga aaacactaaa acttttttgc
    17521 atagccgtat taatgtgcta tgccactagc accagcaagg cgcaaacatt acaagcacag
    17581 catgaaattg taaaaaactt acaagacaga ttaaaccaaa atacagagta tgcaggcttt
    17641 caaactggaa agcaagagta tttccataaa ttctataaat acattagcag aacttatcca
    17701 ggcgaaacaa acagcaatta tataaatact gctgcatatc taacaagtat atcagcttct
    17761 ttttcttcac ccaattttat atacgacagt tcattttatg cgctactttc attgtataga
    17821 aataattggc aatatttgag taataaatat ccaaatacga acaacaataa aattattgca
    17881 gctgcctaca tggttaaaga tataggtaca gaatattttt caaattcatt gtttagcagt
    17941 tcgacagctg tattagatag ctttatacga ataaatcctg actacataaa tatagactac
    18001 acagaagaca gtattttgat aactgacttt ggtcaaaatg acgtgccaat aaattttttg
    18061 ggagaatatg aagtagaatt tgattgggta aatggtgcag aagttattat taatgaaaat
    18121 gactgtaata agcatttcta ttacaaacca gacttagact gcattgatga taccatttat
    18181 tatgcatata aaatttgtga tagttcattt aatttagtta atagctacaa tgatggcgaa
    18241 aacagaatag taatacatac taattatgat ttataccagg ctgcaataac aagtattgat
    18301 actgttttaa actattatga tacacttatt attgatagca gaaattttgt aaatagtgca
    18361 ttgccttttt atgtttcaaa tgattacagt aatgaaaata ttataataat agatagtttt
    18421 tcatttaagt atattgtacc taattcaatt gatactgttt atagttttat tcctattcat
    18481 tacagctgta atgacaatac ttttgacagt ttgccaatat ttattacacg tccaaatttt
    18541 gctaacaaac catttaacga cgatcctaat ttagtttttg attatgaaaa tgcacaagga
    18601 cgtatttatt ggcaatttgt accacaaact tttatagaca gcatacaaaa ttttacaata
    18661 acagatgcat ttttaattgt gcctaatgca agtccagaat ttttgcaaga tgccgttata
    18721 actcaaagtc ctactgatat gtttgtccca aatgccagca tgaaaaacgg cttgcaaaac
    18781 aacccaaaca acatgaacgc aattttagtt aatgccacat ggtccagata tggcattaat
    18841 tatagcggta catttacata taaattacca gcgtttaata cttcaaacga ttttagcacc
    18901 acaataataa gcacctttag tgagaattaa ccatgtaaat ataaccattg gtaaacgtgg
    18961 ctgcggtaaa actaccaaaa caataaactt gattgaaaaa tcaaatttaa aaactttggt
    19021 agtcgatacg tttgatcatg aaaattatag aaaattcaga gctatacagc cagtaatgat
    19081 taatgataaa atttggaaag ctggaaatgt gcgttgttat ggtcacgact ttaaagatat
    19141 tttttatcaa ataagcaata aaataagcaa tgcaatgata gtgttagagg actgcaccaa
    19201 ataccttaag caccctttaa atcaagatgc attgcgcttt attgtcgata gcaaacaaaa
    19261 aaacattgat ttagtattta tgtttcatgg ctttggcatg gtaatgcctg atttatttag
    19321 gcttgccgac agtttaacca tttttaaaac caatgaaaat attgatacat acagaaacaa
    19381 aataccaaat tttgacagcg taaaaaaaga gtttacagcc atacaaaaaa gccaaaacaa
    19441 atacatacat aaaaccataa aaataaacta acatgagtaa ttttgtaata aagacctatt
    19501 caatagtaga attatccttt aaatatggcg tttgtgtgcg cacctttaaa aaggaaatca
    19561 ttaaagcgaa tattgatatt gaattaaata agtataaaaa aatatttttt ccttttgagg
    19621 ttatcaaaat aattgaaaag ctaggagttc cagaatagaa atacattaca ttaaaataca
    19681 ttatgttgca ttaaaataca ttatgttaca ataagataca tgcctttaca agcatgtatt
    19741 ttttttgttt catatttgaa aaaaaaaatc aaaaatgaaa aattacgcaa tcggtttaac
    19801 aatcacagtt gtaggcgcat taatagctat gtacctatgg aaaaaaattt ctgaaaaaaa
    19861 agaagataaa aaagcagaag tagcaaaagt ttaatcaaca attaaaaaaa taaaaaatga
    19921 caaattcatt agtaccaaca atggcagaca aagaatacat tgaaaaaatg gctcaccaat
    19981 taagccagtt tgacgaagac aatttcgacg acgataacga taattatgta ggcagagtaa
    20041 gacctaagct aacacaaggc gtaatggttt taaatatatc aagtagcgca acaactacac
    20101 gtgatttttt tatattttct ggttatttaa tcgacagcat gtcttttgca gccacatttg
    20161 gttcagtatc aactactgca agttatggac aaatagttga tggtggcttt aaaagtattg
    20221 gatcagcaac agcagattta atggcattca gcgacatggg cgcatcttca atcaatgact
    20281 ggatcgcata cataaaaaat ttcaaaggca acaaaattac taagatagtt gtaagaagca
    20341 gcaatccagc agttcacagc cagcgttttg tgattactaa aaaagatcca ttaaatcagt
    20401 tagcagacag aactatcaga ttaagcacat ggcgcaatcc aatgcagcaa gatttgaata
    20461 ctactattat caatgagaca atagatttag gcttacaaga ttttgtaaaa ttttctattc
    20521 ctccatcagt aacagattta gtaatcgaat tacacatgaa cggctatgca tcttaataaa
    20581 tgcatgattt tatgaacaac ttaaaaggtt aatacgtgga tatatttaat catttagcaa
    20641 cgcaacaccg aaatagtgtt gcgtttgttt tttccaaata tggaataaaa aaaccagtaa
    20701 caccaaaaaa tgtacagcgt gcaattttgg ctggtaaaaa cacaccgttt tatcaagatt
    20761 taatgaatgc tatctgtttg caagcattag ggaaaaatta ttatgaagat gatttcgatt
    20821 acaatatcga aaacatggac acagaaaatt ttgaagctga aacagtaggc atcattgata
    20881 cttttgaatt tgactacctg gacagagcag gcagaaaggc aagacgtaaa caaaagaaaa
    20941 ttgtacgcaa gcaaaaaaaa caagctaaaa aagcgagtaa aaacagtgta agaccaaacc
    21001 caaatgcaag catacaagat gctgaatttt cagataatgg aagtggtaat tttggtagtt
    21061 caatcgctga caaagtcgaa actggtttaa atgttgtagg tcaacttgca acggcagctg
    21121 gcggaattgc tggacaattt ttaggcaata aagataatga tgcagcaaca gcagacagca
    21181 acagcaatga agatgaagca gcaacagcaa cagcaacagc aaacagcggc gtaaaaaaat
    21241 acttaccata tattattggc ggcgtggcat tgcttgcagt tgtaggcgtt ttaatttttg
    21301 tacttaaaaa gaaaaaataa acatggtttt tacatcagta aaatatgtta atgaatttcc
    21361 aaacggaaat aactcaccag cggcggttaa tcgcaagact ggtgagttat ttattaataa
    21421 aaaacgctgg agcaacatta aagcagaaca ccgtgtattt attttattac atgaattagc
    21481 acattgcatt ttagatacta gcgatgaaat ggcagtagat gcattggcgc acacctggta
    21541 tttacaatta ggttattcac tttccgaaag cgtttttgca cttacaaaag tattgcatgc
    21601 agatagtgac atgaataaaa aaagaagctg ggcacaatac acacgtgcca aagaaattga
    21661 taatagaaat gatgtattaa attcttatga tggatatgct gaaatatgta gcaatccttg
    21721 taatcttatt acagacatta gcaattttga tgataattct attttagtgc aatatgatca
    21781 gtatgatttt gatataaacg ctgaattggc aaatgtaaaa ggacttagcc gcaaagaata
    21841 tcgtaaaaaa aagcgtgccg cacgattgta caaacgctta ggacgtggta agaaaaaaca
    21901 tgctatagca aacgacattc aagcaggcgc agacacacga aaaacatacg cagataaagg
    21961 catgtacgtc ccaacacgtg cagaaagcat agcaaaaggc gttggcggta caattcaagg
    22021 aatagccagc gcaataggcg gtacaatggg aaaaggcgca gtgcaaagca gctacacgga
    22081 gcaagagcca acagaacaag aacaaaacta tagcaataca aatacagcac gaaaaaaaca
    22141 aacagctact gcaatatccg aaccagtcaa taatagcacc acatcaagta acagtaagaa
    22201 aataataatc ataggtggtt ttgcattaat ggttttgggt attgtagttt ttagtattaa
    22261 aaaataaatc tatgaaaaca aattatttta aaataggact ttatatcgtt ggtacagttg
    22321 tcgtaattgc agccgttttt atgattatta gagcatttgg acgcgcaaaa caaggcgtaa
    22381 gctcaattgg tgacattatc caggataata aagtaaatac cgacatagcg caagccgcag
    22441 gcgttccagt tcaagatgta gataatgcca gaaaaatagc atcagattta gcgactgaac
    22501 tagaactaag aaagggttta acctggtggg aaaaacaaaa aggaattgtt acagatagcg
    22561 aaattttaga cattgccagc caaataaaaa cagcagcgca aatgcgtgtt gtagcggcta
    22621 ttttccaaaa cgaactaacc tacaataaaa atctatacac agaattaaaa cgagagcttt
    22681 ccggctcaaa cttatcgaaa gttccattta ttgaaaccat tttaaaatag aaatatgaaa
    22741 gcatttatca caacaatttt gacattatta ttttttacag ccactgcaca agatagcagc
    22801 gcagtaaata acactataca cgtttttagt gtgttggaat taattaataa tgcaccgtac
    22861 tggcttacta cagccgtaat aattactggt gcattgctta cagcaacaca aacaatttta
    22921 agccgtttcc ctacagaaaa aagtgtaaaa ataaaaggag ttatcggtaa aatattagac
    22981 gttttggctt tgtttcctcc tgacaataaa aaaggcggtg gcacacattt taaataatac
    23041 taaaaaacta aaaatgaaaa attacgacaa aataacacct attgaagcaa taggactagc
    23101 acaaacgatt atcgcttatt tgataaaagg ttttgaaaat atcggtttta gaaaaaacac
    23161 acgcaaagac attgaagcac taaaagagct ggcattgttg caagaaaaaa gaatacaagc
    23221 actagaaaat aaatagaaat ggagcagaaa aacacaaagc cagctttact atctagtttg
    23281 attggaacac cagaaataaa agtttcattg agcaataaaa ctattgcttt attctttggt
    23341 gcattggtgg caagtttaat tataattttt ttagcacatg caattatcaa aaaacagtaa
    23401 acgtataatt ataatagtcg ctattggcgt agtgcttata ttatgcgttt atttttttat
    23461 caaaagcata cgtaaaaaag taaacatgag tgtagaaaat aataatgtgc aggcgttttt
    23521 aaaaatgatc agatatgcag aaggaacagc tggagaaaat ggatatcgga ctttgtttgg
    23581 tggtaaatta tttaatgatt tttctaagca ccctaatata aaagtaccat tttttaacaa
    23641 actaaaagga aaaacggact acagtacggc ggctggtgcc tatcaattta tccgtcctac
    23701 atggttagaa gtttctacaa aattaggatt gtcagatttt tcaccagcca gccaggataa
    23761 agcagcagta gaaaaaatta ggcaaagagg cggtttagat tatgtcattg ctggtgagtt
    23821 tgataaagca gtagataaag taaaattaga gtgggccagc atgccaggtg caggatataa
    23881 gcaaccagaa aaaaaattag cagaattaaa aaaaatatac gttcaacatg gtggaaaatt
    23941 cgcatagcaa tacagtaatt cagcatctga ctgacgcatt tactgatagt gtaaatgtaa
    24001 aaaacatgtt agcagcatca ctattattaa tgtctaaaag catggaaacc ttacccgatt
    24061 tatctattat ttgtaaaaat ataacaacgg ttagcacctc aattggtgcc gtattgttgt
    24121 tggcatacaa tggagtaaaa ttttacaaag aactaaaaaa tacaaacaat gataacagta
    24181 aaaaatagaa tactagacag cgcaacacgt cgcccaatga ataatgtaaa cgtatttatc
    24241 agcgacagca acggcaatga agttagcaga aatggtttgc caaacattgg tagagcgtcc
    24301 gataaaaacg gacaagtaat tattccagtt gccaatgaaa gcgactttgt tacctattca
    24361 tttgtaggct atcaaaagct aactattcct ggcgtaacag cgcaaaagaa aagcaatatc
    24421 attatgcgaa cggtagcgca agatttacca gaaacagaaa taaaatatgt acctagatat
    24481 gaacctacag cagacaaacc aactataagc aacaataatt ataaagctgg taacaataaa
    24541 aaatcatggt taattattgg cggagttgta ggcggtttgg ctattgtagg tttattgttt
    24601 tatgctataa aaaataaaaa atgaaaaaat acagcgcaat aatttttatt acacttttta
    24661 ttgtgttaat ggtagctggt ttaatgattt atcgaaaaaa attattaaac gataggcttg
    24721 aaaaaattgt aggctttgat ttttttgctg gcacacaatt taaaaaagga cagccagtac
    24781 gattaaacca aaacagcgtt ttaaatcaag tttttgaaag cgatggtgaa atgtttgtaa
    24841 gtggtaaaat aacagtaaaa acaagcgaca aactaacata cgttggcaaa tataacagcc
    24901 ttaatgttgt caaaaagaaa ggctggtttt ttgatagata ttttttagta acaaatgatt
    24961 taattatatg aagattttaa agtttttagc aataacagtt ggcattgttt ttttactagg
    25021 cattattttg aaatggctag aaaaatcgac aaaaactacc attaaaatac ttagcataga
    25081 caaaataaat aaaactatgc aagtgcaagt aatggcaaat gacagccaaa cagcgttaca
    25141 tatttttaca tttagtgaaa tgtttagaaa aatttataaa gcaaatgatt acaacttaga
    25201 agtaagtgcc gcaggcgaca tagttttctt acgtgtgctt aatgaacaag gtaaagaaat
    25261 ggcattaaga gttataaata taaaaggaac aacagcacca attattattt attcagaata
    25321 aaaaatataa actatgataa cacaattaag cttatttgac aagccaaaaa gaaagcgcaa
    25381 gaaagcgaaa aaatcaatca aaaaaattag taatcgtggt ttattgcgaa gtacaataac
    25441 ggcacaaaaa aagactattt ctaagctgga aaaacaagta aaagcagaaa agaaaaaaga
    25501 aactgcactg aaaaagaaac ttaccaaagt tcgcagaact gcaaagccta ccaccagcag
    25561 aaaaaggaaa aaataaaata caaaatacca cactatgaaa gcaaatcata tagctagaaa
    25621 tgctagtatt aagtttttag gaaacaagta tgagttattt tttcaagcaa taaattgtga
    25681 ccatatagca gataattata gaggtttttt ttcatctcac gatgttaaat ttgtagaaat
    25741 tgtcaattta ttaaaaggcg atatttgggt acagaacacc aaacagacca acaaatacaa
    25801 aataatatct aaatacaaag gaaaatatta cagaaccata gtaacgctat ccgaaagaat
    25861 agacttaaac aaggctttca ttgtcgttat ttcttgttat atttgtaatg ataaaacaga
    25921 acaaaatgct tacgaaacgt acaccattga aaacaaaaaa aggtacagat aagccaacta
    25981 aaagaaatag tcttttgcca aagcctgtta aaatggctgt atctattaaa ggaaaatggt
    26041 atgatataag caatccaaat aaacctattt ttttaggata atatacttga gtgtggtatt
    26101 cgggtttgaa tgtaatatta tgtgtggtag catggtttta ctaaagcgta ctttttcagt
    26161 acgctttttt ttataatgct ggatatttat tttttaaatc atcacaatca agcaccccca
    26221 tttctcgtaa atattcattt acctggtcca acgaatgatg acggagctgt aattgtaaat
    26281 cttttaattt gatgccacct tttacggcca tgtatgcgcc agtatgtttc cagctataaa
    26341 aagaatatct attactaaag cctaatttag ctagtatttt tttatgttct ttgcttaaat
    26401 aatctctttt tagttgctcg ttatttattt gtataagata atcattttca tttttattgc
    26461 ttagtgctat aaagtcagat agaaaacaat taggaattac aacgacttgc gtttttttat
    26521 tttttgaaaa tgaagcatct atttttatcg ttccatctac agtatttatg tgttctatct
    26581 ttaaaaatcg tatttcattt ggacgtataa aacagtaata ttgtaattga caagcaaacc
    26641 ataaaattct attttgtttt attatttcac tctttaatac ttcaatctgg ttagtattaa
    26701 agtacatttt accctttcta ttttctggca gtttttttat ttcctcaaat ggattaaatc
    26761 taattttatt ttcttttaca aagtctttaa acaatctttt aaatgttatt atataactgt
    26821 ttacagtagt gttgtttttt tgtagcaatt cttgtaaata ctttgtcgca atttttttat
    26881 caattttcct taatttatca attctattac gacggcaaaa atctaaaaaa tcatttattt
    26941 tactttgata agtagcaata gactttttac gcaaatacgg tattctcttt ttaagtgcaa
    27001 catcaaaaat attttgtaag tcctctatgt caaattctat tttaggattg gaaacatcaa
    27061 gtgtgtttaa caatctcttt gctgcataaa atctttcatc agcgtttgaa tagcgattaa
    27121 taccaccata taatttataa ggaaataaaa caccagcttg atttagataa tacaaaaaat
    27181 accaacgctt actagtacag ccattattac tgtataatgt gtatcctttg ttttggagaa
    27241 aaaatttttt ttctattgtc ttatccattt taaaatttta aaatgaacca cgcaatagta
    27301 attaattaaa tatcttacct ttttaacaaa tgagaaatta aataaattcg catttgtttt
    27361 gtttttagcc tacaataaat tcattatcaa tgaattgcaa gtaatttgcg gagacggcga
    27421 gattcgaact cgcgatacag tttcccatat acacactttc caggcgtgct ccttcaacca
    27481 ctcggacacg tctcctttat ttagtagtga gtaattagat tttgaaattg gaataagaat
    27541 tcattttcat tctttatatt caatgctcac atctatctct attgctcact tatatttttc
    27601 cagatagtac accacgagaa ataacgatgc gttgtacttc agatgtgcct tcatatatct
    27661 gcgtaatttt tgcatcgcgc attaatcgtt ctacatgata ttcttttaca taaccataac
    27721 caccgtgcac ttgtacggct tctgtcgtta cgcgcatggc aacttcagat gcatataatt
    27781 ttgctgttgc agatgcaatt gcataatctt gatgttgatc ttttaaccaa gcagctttat
    27841 atactaataa tcgtgcggct tcaatttgcg tagccatatc tgccaatttg aattgtataa
    27901 tctgatggtt tgagatttct gtaccaaatg ctttgcgtac ttttgaatat tctactgcca
    27961 attcgtatgc gccagctgca atgcctaatg cttgtgctgc aatgcctatt cgtccacctt
    28021 ctaaggtttt catggcaaat ttaaagccaa agccatcttc accaattcta ttttcttttg
    28081 gcactttcac atctgtaaat aatagagaat gtgtgtctga tgcgcgaatg cctaatttgt
    28141 cttcttttgc accaatttca aagcctggca ttcctttttc tactatcaac acattgatgc
    28201 cacgatggcc tttttcagga tgcgtttgtg caattactaa atacgtatct gctttgccac
    28261 cattggtgat ccagttcttt gtgccgttta ataaatagta atcaccttta tcttctgccg
    28321 tcgttttttg tgatgtggca tcgcttccag cttctggctc gcttaatgcg aatgcgccta
    28381 atttttcacc tttagctaat ggcgctaaat atttttgttt ttgctcttct gtaccataag
    28441 cttccaagcc ccaacatact aatgagttat tcactgacat ggctactgag caagatgcgt
    28501 ctaatttgga aatttcttcc attgctatac aataagaaag cgtgtccatg ccaccgccac
    28561 cgtatttggt gtccaccatc atgcccataa atccaagctc gcccatttgt tttaaaaact
    28621 ccgtaggatg tatcatatcg cggtcgcgct cgataacgcc tggttttaaa acattttgcg
    28681 tgaaatttct agccatttca cgtatttgtt gatgttcttc tgtaagttga aagttcatga
    28741 taagaaattt gagtgtgcaa aaatacgatt taatttgaaa attagcgaat ttgaaaacaa
    28801 gtgaatttat ttcctttcac caattatttt tgtggtggaa aaaccggata ataatggaat
    28861 aatcgtgatg ttttttgcaa attggcgccc aataatttca tctattttat aatcgcctcc
    28921 ttttactaaa acatcaggtt gaatttgttc gatgagcagt aatggtgtat cgtcggaaaa
    28981 tgagataaca tagtcaacat catctagtgc aattaatttt tcaattctta tttctaaggt
    29041 ttctatcgga cgtgtttcgc cttttaattt tctaacagaa gcatcattat tgattgcaac
    29101 gataagttta tctcctagtt tcttggcttc cttaagtagg tgcaaatgcc cttgatgtag
    29161 caagtcgaaa cagccgtttg taaaaacaat ttttttattt tctctgtcga gatatgatag
    29221 taattgctgt acatcagtaa tgtgtttttt actgttgagc atgttctgta gtttgctttt
    29281 tacttttatt atatattgcc aagaagataa gacttaaaat accacctatg attacgaaga
    29341 aagtttgtac agaccaggaa atccagccat aagaaagtgc aatgatttcg ccaatgccat
    29401 agcttaccaa aattttttga ataacaattt ggaatgcacc tacgccacct tgtgtcaata
    29461 ccattgcaaa tccaccaaag aacacagagg ccattgcagc agtaaaacct aaatgagcag
    29521 tttcttccat gcatctaaat ccaaaataag gcattaaaat ataacatatc catattgcaa
    29581 aagtgtggaa tataaattcc catttttctt ctaaatcttt tatggaaatc aatccttgcc
    29641 acagtccttt aagctttgca ataatgacat gttcttcgcg tacaatttcg tcgtgtacgg
    29701 cttttttctt atttaaaaaa tatagcacaa ttccaccgat aattatggcg attccgccag
    29761 cgattccaag ctttataaaa ttaatttttg cagcaatttc tgcgaagaat gaataaaatc
    29821 gttgatattc taaaataaga attagtacac ccagaatcat gatgcaaaat aggtcgataa
    29881 ccctttcgag taccatagtg ccaatggact tttctactgg cactttttca tatttataca
    29941 atatagtgca acgcatgatt tcgcccaagc gtggaaataa aaggttgaaa aaaaacatag
    30001 ccattactgc agaaaaggtg ttccaataat ttggagaata gcctataggt tttagcaata
    30061 aacgccagcg ttgcgctctg aaaaaattac ttcctaatcc aaatatcatt aacagtaaca
    30121 accaaaaata atcggctttt ttgaaggtgt caacaatcat ttgtttttgc gctgtactta
    30181 catcttttaa tgaccaaaag actaagccga cgccaattcc taaagaaata ataaatttga
    30241 gcaggtttcc gatatgcttt ttcaagtaaa aactatttgc tcacaagata agcttaatgt
    30301 tttacttgtt ggttattttc atctacataa actgcaacag gtgtatgtga ttttgcttct
    30361 tcgagtggca acatgccgta tgcgatgacg ataagcttgt nnnnnnnnnn nnnnnnnnnn
    30421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30481 nnnnnnnnnn nnnnnnnnnn tgtgattttg cttcttcgag tggcaacatg ccgtatgcga
    30541 tgacgataag cttgtcgcca agctgtactt tgcgcgcagc cggtccgttt aggcaaattt
    30601 gccctgagcc acgctcacca gtgataacat aagtttcaaa acgctcacca ttattgatgt
    30661 ttacgatttg tactttttca tgtggtaaaa taccggaagc atctaataaa ttctcgtcta
    30721 tggtaatgct gcccatataa ttcaaattgg cttcggtcac tacaacacgg tgtagctttg
    30781 ctctaaaaac gtgtactaac atacctttat aattgagtgc aaagttatgg atatttatga
    30841 atagtcaaat tatggaagga ttaataatta agcacctatg acacaatgac attgtcaatc
    30901 aagcgtacat cgccaacttt tgcggcaatc aagagtgtaa ttggtgtgtt ttcttgatag
    30961 ctgtcaatag tttgtaaagt tttgccattt acagccacaa tgtactctac actaataagt
    31021 cgattttggt gtaatgtttc taattgttct tttatcaacg ccaacaaatc agcatgatgt
    31081 atatgttcct ttatggtaaa aagtgctttt gaaagttgta cggctgcaat tctttcttcg
    31141 gcatttaatc gtgcatttct actgctcatt gccaatccgt cttgctcgcg aagtgtagga
    31201 catatttcta tttgtacagg aagttgatag tgctctacta actttgtgag taccatacat
    31261 tgctggtaat ccttttgtcc tataaataaa atatcaggtt gtacgatatc caataatctt
    31321 ttcacaactt gcaatacgcc ttcaaagtgg ccaggacgat gtgcggcatc taaggttttt
    31381 ccaataaaac cgaggtcgat acctgttttt tctaaggtgc cattcggata aatttcttcg
    31441 acagtgggca caaataccac atcgcagcct gccgatagta atagctctaa atctctgtca
    31501 atttgtattg gatatttgtc aaaatcttgt ttgtcgttaa actgtgttgg attgacaaaa
    31561 atactgcata ctgtaatggc agttttggtc tttgaagctg ctattaaaga caagtgcccg
    31621 tcgtgaagcg cacccatcgt aggaacaaac ccgacaaacg tatgtcgtaa ttcattgatt
    31681 tccaataaat atttttgaat ttcggctaca gtttgaaaaa tgcgcatctt gtctatttta
    31741 cattttttaa acaataatac cactttaaat gcgtaccttt gcaaactatt taaccataac
    31801 ctaaaaaatt taacagcgta gtagcgaatg gaaaagaaaa aattattgta tgtaacgcaa
    31861 gaaatggatc cgtatttagt attgacggat gccgcacatg ttgtaaaaaa attagcacct
    31921 tatataaacg ataatggact agaagtgcgt gtgttaatgc cgcgatttgg cattataaac
    31981 gagcgtagaa accgattgca tgaggttgtt cgtttgtctg gtatcaatat tattatagac
    32041 aatgatgact atccattaat attaaaagta gcctcattgc ctggagcaag aattcaagtg
    32101 tatttcttag ataacgaaga gttttttaag cgcaaaaccg tattcaacga tgaagacgag
    32161 aagtttttcg atgataatgc agaacgtatg gttttctttt gtaaagctgc attagaaacg
    32221 gtgaaaaaat ttggctggca tccaaacatt atacattgcc atggctggat gacaagtttg
    32281 atacctgtgt acatgaaaac acagtacgct aaagatccag ttttttctac aacaaaaatg
    32341 gtgtatagcg tttacgaaaa aacattttca gaaaagctac acaagaattt tttgaaacta
    32401 gcgttaatag gtgacgatat taaagataaa gatttggctg cttttaaaga tttaaccaat
    32461 gaagcactaa cgcttggtgc cgtacaattt gcagatgcta ttattcaagg tagcgaaagc
    32521 atcaaagaat tggcagctat taagaaaatg gcaggtaaaa aaccgttttt aacctatcag
    32581 gaaatggaca acattctgcc caactataat gagttttatc aatcgctttt acaataaatg
    32641 acactattct cttacatgaa aaaatgctct tttctaacaa tcatcacttt acttgttttt
    32701 atttcttctt gtaaaaaaga cacgaatata ggtgcagata ttttgccacc agatgatttg
    32761 ttaaatgtga aattcacaga cacacttacc ttgtattcta aaacattggc agatactttt
    32821 ttgagaacag ataaattagc aaaaaattat ttaggtgtta tcaatgatat aaaattcgga
    32881 acacaaaagg caagcattgt catggaattg gacaagccga ctagtgtata tgacgatact
    32941 ttaaatacaa gctatactat tgattcggtt gtgttgtttt taaagtattc atcaatttat
    33001 ggtgatacga cagaaccaca aagctttgat gtaagtacaa ttgggaataa gataaatgaa
    33061 aattcagcat attattacaa tactactcaa tttccgagta caggcttaat aggtagcgtg
    33121 agcaattacc tttttacacc tactaccaat aaagtacaca caactatttc agatactgta
    33181 ggtgttgctg gaatattgag aattaaatta gataattctg ttggaaatac aattattaac
    33241 ttagggcaaa gtgtgcttcg tgattcagca gcatttaaaa atgcatttcc tggcattcgt
    33301 attgaaaatt cgaacaactt aggacgtgca atggcagaaa tagatctgaa ttctttgtat
    33361 agctccattg ctatttttta taaagataaa tacggtacta aaaaagacat gagattattt
    33421 acgtcaatag tgaaatatac aaatggcgtt gctggcacgc gtgtaaatgg cattaatttg
    33481 ttttctaata cattatcatc agcattgcaa aatacaattg cttctggcga attatctgat
    33541 tcgataagtt atgttttagg acaaggaggc gctaccataa aaataagcat gcctacaatt
    33601 tcgaatttag gaaatattgc agtgaataaa gcagttgttg caatggcaca aattattccg
    33661 aatgcttcga atttaaatac accatattat ctggtgttat taaaacacaa taacgatggt
    33721 catttagatg ttttaccaac tggcgatggc gtaggtattt tagatacgac aacaactgat
    33781 ggatttggca acaaaatagc tcgttataat ttcaatatta caaagtatgt acaagccgtt
    33841 gctaatggca caatatcaaa cacagattta tacttaggaa catatcgctt tgcaggaact
    33901 gatggcactg tcaatgtctt aaatagcatc gtcaatggca ctgtgctgag tataggatat
    33961 tctcctgctc gagtaattgt tgcaggtgct aatcattcag atgcgagata taagatgaaa
    34021 ataaatctga cctataccaa gattaattaa tttcattttc ttatcttttt acattaattt
    34081 ggtacagcat ttgcatcact cttactaata aaaatttatg tgcggaatcg taggttatat
    34141 cggaaagaaa caagcctttc caattattat taatggacta aaaagattgg aatatcgagg
    34201 atatgacagt gctggtattg cattgctcag cgaccagaat gcactaaata tttataagaa
    34261 aaaagggaaa gttgccaatt tagaagaagt agcagaagcg aatgatgtat caggaaatat
    34321 aggcattggc catacacgtt gggcaacgca tggcgaagcg aatgatgtaa atgcgcatcc
    34381 tcattttaat gcaacagaag acttagcagt catacacaat ggcatcatcg aaaattatta
    34441 ttcgctaaaa acagaattgc aatctcgtgg ttatgtgttc cgctccgaaa cggataccga
    34501 agtattggtg catctaatag aagaaatcaa aaaaaatgta ggctgtccat tagaagaagc
    34561 agtgcgcctg gcactcaatg aagtgattgg tgcttatgcc atagttgtga tttcaaaaaa
    34621 ggaaaataat aaattaatta cggcaaaatt aagttcaccg atggtaattg gtttcggtga
    34681 aaatgaactc tttgtggcgt cagatggctc accgttaata gagcatacta aaaaagtgtc
    34741 ttatttagaa gatggtcaga tggctgtgtt gtcgattgat ggcactgtag aagtgaagtc
    34801 tattaaagac aatacatcct atgtgcctta tatcgatgaa ttgcaattaa aattagagca
    34861 aatcgaaaaa ggcggcttcg atcattttat gatgaaagaa atacatgagc agccacaagc
    34921 cattaaaaat agcatacgag gacgtatcga tagtcaacag ttttgggtaa acttaggtgg
    34981 tgttcgcgag catgaattac gttttaaaaa tgcctctaaa attacaatcg ttgcctgtgg
    35041 cacatcttgg cattctgctt taattggcga gtatttaatt gaagaattag cacgtataaa
    35101 tgtagaagta gaatacgctt ctgagttcag atatagaaat ccaattatca cagaaaacga
    35161 cgtagttatt gctatttccc aatctggtga aacagcagac acacgcgctg cattagaatt
    35221 agcaaaagca aaaggtgcat taacctatgg tatttgcaac gtagtaggct cgagtatatc
    35281 gcgcatgacc gatgccggtt cttatacaca tgctggccca gaaattggtg tagcctcgac
    35341 aaaagcattt acaacacaag taagtatctt agcattatta gcactgcaat tggccgagtt
    35401 aaaaggcaca ttatcgcaaa caaaattcca cgaatatatt acagaattag aggctttgcc
    35461 ttcaaaaatt gaatctattt taaaagcaga accatacatc aaaaagatcg cagatacttt
    35521 ccaaaatgcc actaatttct tgtatttagg tcgtggtttt aattttcctg tggctttaga
    35581 aggcgctttg aagcttaaag aaatttccta tatacatgcg gaaggttatc ctgcagcaga
    35641 aatgaagcac ggtccaattg cattaataga tgagaatatg ccagttgtgg tattggcgcc
    35701 tcatacacaa cattacgaaa aagtgttatc aaatattgaa gaggtaaaaa ctagaaaagg
    35761 acaactcata gcagtagtaa cggaaggtga tgcagaaatt agtaagattg cagaatacgt
    35821 aattcaaatt ccagatacac ctgagccatt tacacctatt ttatcagtca ttccattgca
    35881 attattagcc tatcatatcg cagtgatgcg aggttgtgat gtagatcaac cacgaaattt
    35941 agctaaatct gtaactgttg aataatgcaa ataataaaaa ccctaagctt cttatttttt
    36001 ttatacatct cgtttgtttc acatgcgcaa acaataccgt ataacgtaca aaaaaagaaa
    36061 tatccattag gtgagcaatt ccaaaatcta ttacctttaa aattagaaaa atggaatcga
    36121 tatgcttatc acgatttcgt gcctaatcat gaaaccggct atgtctatta tcgcaaagat
    36181 aatcaacaag tgtatcttac ttttggtaaa gcatacagtc agtcaggttt gaatgctgaa
    36241 tggtcaaaaa tttacgacga cgctaaagaa agcaagccag gtaaattagt acatcataat
    36301 cctgcaacta ctgctactaa atatctctat gtgaatggta ataaaacttg tttttttgca
    36361 tggacacgta atttctatta tttcactgtc gaagcgaaaa gcaaaacact tgtcgatgaa
    36421 tttatgaagg tgttccctta ttaaacattg cctttcattt ttttcttgtc aaattaatat
    36481 cttatattgg atggaaaata tcatctaatg caaactaatt cttccgtttt tttaggagat
    36541 aaattctaca atcgcgaaca cttaaatttt attttatttg atgtgttaaa agcagaaaga
    36601 gtaactgctt acgaatattt ctcagaccat gataaagaaa ctgtgcaact ttatttggat
    36661 gcgtcagacc agtttgcaaa agaatatctg tttccacatg tagtagaaat ggatagagtg
    36721 caaccagaat tagtagatgg tagaattcga gtgcatcgcg cgcacaaaga cattatgcgt
    36781 atcagtggtg aaaatggttg gataagtatt cttgcaagta aggaagttaa tggtatgcaa
    36841 atgcccagtg tttttagtac agcagcaagt tttatacttg gcgcagcaaa ctattcaggt
    36901 tgtgtgtttc caggcttaac catgggtgca gcacatttaa ttgaaacatt tgcagataag
    36961 aaatatcatg ccatttattt accgaaaatg tttgctggcg aatggcaagg taccatggct
    37021 ttaactgagc caaatgctgg aagttcatta agtgatatag tgtcttcagc agagaaacaa
    37081 gcagatggta cttataaaat tgttggacaa aaaatattta tttcgtgtgg agatagtgat
    37141 gcagtagaga atgtggtaca tcttttatta gcacgcataa aaggcgcgcc aacagggaca
    37201 aaaggcatct ctatgtttat tgtgccgaga gaaagaataa atgaaaatgg tgcattggaa
    37261 tataatgatg taacgagcat tggcgtgtat cataaaatgg gctataaagg tgcaccaatt
    37321 gcacacttga gttttggtga aaataatgat tgcaaagctt ggcttgtagg tgaagaaaat
    37381 aaaggtttaa cctatatgtt tcaaatgatg aatgaagcac gtatatcggt gggcttgcat
    37441 gcaacttcta ttgcatcagc agcatattat gccagtttaa aatatgcaaa tgagcgatta
    37501 caaggcaggc caattacaga aaaaaatcct gaattaccac aagtaccaat catcacacat
    37561 gctgatgtga aacgcatgtt gctatttcaa aaaacattta ttgatggcgc tttgtgtttg
    37621 gaattacaat gcagctattt tgctgatatg gaacgtgtga cagaaggcga cgaaaaagaa
    37681 aaatataatt tgctgttgga attattgaca ccagtggcaa aatcgtatcc agcagaaatg
    37741 agcaattttt ctacatcaac agcattgcag atttttggag gttatggctt tacaaaagat
    37801 tttattgctg agcaatatta tagagaaaca cgcatacacg ctatacacga aggaacaacc
    37861 gctattcacg gattagattt gttgggaaga aaagtgatga tgaaaaatgg caaagcagta
    37921 atgtatctaa cacaagaagt aatgcaagct attgcgaaag ccaaaaaaca tgaaaattct
    37981 aaggagcaag cagaaaattt agaaaaacaa ttggctaaat tgcaagaact ttctatgcac
    38041 ttaatgcaag ttgctcaaca aaaaggagtg gaggcttatt taagtgatgc tacattgtat
    38101 ttagagttat ttgggatatt ggtgttagct tggcaatgga taaaaatggc aaacacttgc
    38161 aatgaaaatg aaagcataga taagcatttc aaagaaagta aatttttgtg ttttaattat
    38221 ttctttgaat atgaattgcc aaaagtacaa ggtatgttta ctcgtttaaa aagcacaaat
    38281 catattacag ttggaataga tagtagcttg attgattaaa ttgtctgaac tatgatttat
    38341 ctgatttaaa gattaagatg attttgcttt gtgtacttcg cgcaataact ttgtattctt
    38401 tgcaataaaa aatatactat gaccactatt caacaaatat cactttacat catggctgca
    38461 ttttatgcag gcggcggaat taaccatttt gtaaacccta agttttattt aggtataacg
    38521 ccaaaattct ttccaattcc taatgtatta aactacgtaa gtggcgttgc tgaaattgta
    38581 ctagccatcg gcttgctatt tgcttccaca aggcaaattt ctgcatattt aattattgcc
    38641 atgctgattt tattttttgc ggttcatatt tcacatttgt ttcatccacc aaaaatggca
    38701 caaggcaaag aatggttttt gtatgtgcgt ttagtattgc aattcgccct aatttattgg
    38761 gcttggcgcg ttggtaagta ttgagaaatt tgtttccgta acacgtaatc attcatccaa
    38821 tatttaccga tagcaatatc tactttttca tgcaattcaa agcccatttt cttgtagaaa
    38881 tacattgcag gattttttcg attgacattt aattctataa aatcgagctt gtttttttga
    38941 gcaatttttt caacttcttg caataataat tttccgatac cttttccatg tgtgtctggt
    39001 tttacatata tttttggaat gcaaatgctg ttgtcttctc gaagaatata agatataaaa
    39061 ccaagtattt tctcattttc cgaacatatc aagaatgtat taccattttg catttgttgt
    39121 aatatggctt cgcttgtgta catacgttcg tacataaagt ctatttgctc ttgcgaaaga
    39181 ataggtgcat aggtttgttt ccaagtagct gcagctactt caataatttt tggaatatca
    39241 ttaacaaaag ctatccttat tttcatagca taaattattt taacacaaaa aaatagcgct
    39301 attaaatatt ttatcttaat tttaatttaa acaagattaa aatgtcagca acagagattt
    39361 taaagacaaa gctacataca tttattgaga atatttctga tgaaaaaata ttgaatgctc
    39421 atgctgtatt gttacaaaat gaaatagaac aacaaacaga agattggaat accttaccag
    39481 cagaattaaa agaagcatta caacaaagca tttcagagtt tgacaatggc aactatgaac
    39541 ctattgaaga tgtgctgacc aaattaaaaa agaatggcat ttaagataat aatttctaaa
    39601 agtgccactc aatcgttgca ggatatttta gactatacat cactaaattt tgggaaaaag
    39661 aaacacgata ttttattgaa aaaaatagaa gatgctattt ttacaatttc cgaacatcct
    39721 ttgttgtttc ctaaaataaa agaaacaatt catcgagttc taattatcaa tgaaatttct
    39781 atgttctatc aaattaaaag cacatctatt cgaatattat tattttggaa taataggcaa
    39841 aatcctaatc gattaaggtt gtaaaattca ttcaattctc atttttattt actaattttg
    39901 aaaatatact ctaggagtat cttatttttc tttttgagaa atgagattga gaaatatcct
    39961 ttgaacctga tttgg
//
LOCUS       DAOQQV010000009        39709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_280_length_39709_cov_7.487996, whole genome shotgun sequence.
ACCESSION   DAOQQV010000009 DAOQQV010000000
VERSION     DAOQQV010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 39709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39709
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_280_length_39709_cov_7.487996"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /locus_tag="PLL59_02030"
     CDS             <1..1802
                     /locus_tag="PLL59_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY22609.1"
                     /translation="STPKPFYFGAKFVNNTGSTIVSAAVSYRCEQWRRGNSTNGLKDT
                     LFVEYSTDADSVRLGTWTRVPSLTGSSVNTSTTTGALDGNAVFTNVAGNITGMAVPNG
                     ATFWIRFNDYDIPGSEDLLAVDDFSVSFTTGTVAACTEPTNSVSNLVLSATSTTAVTG
                     SFNATVPASDGYLVLLDSNAVTPTITDATAYSNGDLVGTAVVVSNGANTTFNKSGLVQ
                     NTIYYAYVFPYNNAGCLGGPNYKTSAPANDSAKTLVDACPEPATNPTNITFTLVGDNS
                     IEGQFNRTTPPADGYIVVQSLSSNIGYPADSTNYNVGDSIVSGSFKSKVADVSSSAND
                     TTFVITGLASGTKYNVAVIPYNLCGAFKNYRRTASNGVNRDDTTTTGTPPLTDCVQPS
                     GVSNSSIVKLDSTTSTITIKWKNSSNGDSVMIVAAPSSVGSISIRDTMNYPVGTIIPS
                     SNATSAKVYFRGTDSTCVLTGLSQNTLYKIFVIAFNNKNCLNGPNYGGLATAFAKTAL
                     STGVKYNNVEAEFNLFPNPTNTGAIYVKFKSPIKEVATLEVVDILGRKLIAQQLAPGN
                     DMESIDVSSLSKGTYILNIVYKGSNNVSTFIVE"
     gene            1880..2122
                     /locus_tag="PLL59_02035"
     CDS             1880..2122
                     /locus_tag="PLL59_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22610.1"
                     /translation="MNRRFFLYFLLIFLISPLLVKAGPGNYYNIDSSKSCAAFKSILA
                     SRLALGSVSINYGDVDFYFNRTDSKIQSQQNQEVVV"
     gene            2122..3051
                     /locus_tag="PLL59_02040"
     CDS             2122..3051
                     /locus_tag="PLL59_02040"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HMY22611.1"
                     /translation="MVDRYSGERPNGLDSCNYRYDVDFCSSGGTASTQCVCYVKEHSF
                     PKSWFGGNVIPMYSEMHLLLPADNYTNNAKSNFPIGYVKTPSITSYNGTKIGSSDDSL
                     NYGFNATSVFEPIDQFKGDFARIYLYMVTRYESVVASWISNSTANDVMAGNSYPALDP
                     WILKLCVKWHKQDPPDLLERNRNDSVFVIQGNRNPYVDYPHWVEKVFGVDGIDTSCVI
                     TAVRTNSNSFTSAVFPNPANDRLQIQTVLPFPTKEASISVFDYLGRCILSHKINNGTV
                     ENNTINIASLPRGIYLLQIENDGATSMTKFVKE"
     gene            complement(3065..4621)
                     /locus_tag="PLL59_02045"
     CDS             complement(3065..4621)
                     /locus_tag="PLL59_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HMY22612.1"
                     /translation="MKKMLFILQIICVQLLFATYPIKDVQWTSFAHSSTFPASVKKVS
                     IITFGAKADNITDNSIAIKNAIQSFNGNYGIIEIPAGKYLFKKSISIPSNIILRGAGS
                     DKTILEFNLNGNSDLISVQGTTTPISTAITTNTTKNSSTIKVASSDKFAVGDFVVVKQ
                     NATHVLTSNWAYPYFIQILKIKKINGNEISFDANLNYVYSTNNAPTVQKINPAQQVGI
                     EALKIKRTDATSQQTSSIFFNYAVNCYVKGVEMENSNYAHITLQSTANTTISGNYLHD
                     AFDYGSGGKGYGIVLQFGANNNFIYDNIAKHLRHSFLLQAAANGNVIAYNYSYDPYWE
                     QGWFPSNAAGDIVLHGNYSYANLFEGNIIQNLVIDNSHGINGGDNTFFRNRIENYGVV
                     MNNNSGDNMNFIANEITGSGLLKGLFTLSGNQIAIANNIKGDLESGTVTEQSLINKNY
                     ASKIGMPYAVGSWKNDAYIRNTKAVKTIVNTSKAEVSTSNTSISTETKSTEIKKKKCT
                     KKRCKRKHKK"
     gene            4883..5278
                     /locus_tag="PLL59_02050"
     CDS             4883..5278
                     /locus_tag="PLL59_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY22613.1"
                     /translation="MKPYNNIALLLLLLLMSGITYAQSELVAENASFTPSNAVALFKS
                     DDKITISEFVQDLTNANPTVNVKCQIPLAVQVKVFNMDGNMAKLETYQLEKGLTELNL
                     KLDDLAAGSYMVQIYSKEGSAVRRFVKPN"
     gene            complement(5384..6523)
                     /locus_tag="PLL59_02055"
     CDS             complement(5384..6523)
                     /locus_tag="PLL59_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HMY22614.1"
                     /translation="MSRKPQQGPLQGIRILDLSRLLPGPLATQMLADLGAEVIKIEDP
                     KAPDYTRFFPPLKGSQSLNYISINRSKLSVSLDLKSDEGKEKFFKLVKTADIVVDSFR
                     PGVLQKMGIDYAAAKIHNDKIIYVAVTGYGYSGAYKDKAGHDMNYLGYSGILGLTGDK
                     EKPIIPACQVADVAGGSYPAVVACLSALWARERTGKGQFVDVAMTDCVMPLLSMQFGE
                     SLNTNIRHRRGEPMLSGGMANYHLYKCKDEKWIALGSLEPKFWQGVCMMLGKPDWMPR
                     MMPIPEEVEKLKKDLQDIFITKTRDEWSAIAAPLDVCLTPILELEEVENDKHLIERDM
                     FFEVEHADYGKVKGINQPFKFSETPAKPSWAAPLMGEDNALLDNI"
     gene            complement(6668..7393)
                     /locus_tag="PLL59_02060"
     CDS             complement(6668..7393)
                     /locus_tag="PLL59_02060"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HMY22615.1"
                     /translation="MKRFWIIIVVSIFSFGCTTQQIQETVSAVLGTTYGDPSEKEAGD
                     GLKDALGIGITKGMSLLSATDGFLGNDLVKIPWPSEAEKVKDAMLKLGMQKQVDNVTT
                     SLNRAAEKASGVAIDVFLQVLKQMTVQDAIQIITGGNGAGTAYLKKTATPILTEKFRP
                     IVDQSLGQVNATKYWSDATTVYNKIPFVKPVNTDLTGFVTDKALEGVFKMIEKEENNI
                     RANPLSRTTELLKKVFGFADRKK"
     gene            7465..8715
                     /locus_tag="PLL59_02065"
     CDS             7465..8715
                     /locus_tag="PLL59_02065"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HMY22616.1"
                     /translation="MKCTILNIGDELLIGQTLNTNAAWMSQKMNEIGIDVIHHISLSD
                     ERNDIENCLNNALNDSTIVLITGGLGPTSDDITKQVLCDFFGGKLVFNDAAYKNIERI
                     FEHRKKLIDEATKQVAFLPDNCLIIPNNNGTAAGMIFNKDGKTIVSMPGVPHEMKAML
                     DEALIPYLKQTYQLPFILHCNILTAGVGETQLASRLEQFEKNKHSEIKLAYLPSIGKV
                     RLRLTIKGNDKLILEQHLSQAKSEVCHVIDDYIYGFEDDTLEQKIGALLLEKKYTLAT
                     AESCTGGYLAHLITSVAGSSSYYKGSIISYANETKENLLQVHKNTLLEYGAVSKQTVN
                     EMLAGALQQLKTDVAIAISGIAGPDGGTTEKPVGTVFIGVGTHEQKIIKRFALSSHRD
                     RNIQAAGIFALEMLRKFLLNKLNE"
     gene            8774..10198
                     /locus_tag="PLL59_02070"
     CDS             8774..10198
                     /locus_tag="PLL59_02070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HMY22617.1"
                     /translation="MAEYKLLMPKMGESVIEATILAWHKNVGDKINEHDVILEVATDK
                     VDSEVPSPVNGVLKQILHDIDVAVGVGEPLAIIVVDGDAPAQYLPEKTKTETASQKEL
                     PIVKEEIIIENPTPVNIAETKQELVQQIEKTVATAITQTIASTSANTNKYYSPLVLNI
                     AQVENISMQELDTIQGSGIDNRVTKEDILSYIELRKHKPTPKVETTTSVATQQKSSTP
                     ISDKKEEKSTVANTDSTDLSSNIEIIEMDRMRKLIAQHMTNSRKTAAHVSSFVECDVT
                     NIVRWREKNKNAFQKREGFSLSFMPIFVEAITKAIKDFPMINVSVDGDKIIKKKDINV
                     GIATALPNGNLIVPVVKQADQKSLLGIAKAMNEIVLKARKNALQPDDVMDGTYTISNI
                     GTFGNIIGTPIINQPQVAIMAVGAITKKPVVVELDGQDVIAIRHMMYLSHTYDHRVVD
                     GQLGGLFVKKVADYLEKFDLHQEI"
     gene            10223..10816
                     /locus_tag="PLL59_02075"
     CDS             10223..10816
                     /locus_tag="PLL59_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY22618.1"
                     /translation="MKKKLDIKECKEILYNVLKQYSNLEDVILHEMVANFELEVFPKN
                     SVIIPKNCTNNKLFFVLNGLVRIYYEAEGKEITYDFKEENSFFINGYTIFTKLPNFDL
                     HVAIEHTVCLSANYDKMEAIMEKYHQVEHLGRKMVELYYANYLIANYNKMFLSAVERY
                     ELFAKDYSSLMLRLPLRFVASYLGIAPETLSRLRSKY"
     gene            10824..11420
                     /locus_tag="PLL59_02080"
     CDS             10824..11420
                     /locus_tag="PLL59_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY22619.1"
                     /translation="MKRKPINIEEHRGKLFSLFMRLGPKTPANELQELVDQFEIGMFS
                     KREIVLQAGEFSDTVYFIVSGLVRIYYVKEEKEITNWFIKENMMFAATYSILTGKANY
                     SSYEALEDTVVLKINYSTLEKYYAKYHSIEHIGRKLIEYYYGTFMKKTFDVLFLSAEE
                     RYSLFMKDHGDLINRVPLRFIASYLGITQETLSRLRSK"
     gene            11557..12168
                     /locus_tag="PLL59_02085"
     CDS             11557..12168
                     /locus_tag="PLL59_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY22620.1"
                     /translation="MTSQAHNLQEKNARALLRQILSGTAHVDSNKLDALVNLFEMVSY
                     KRNHKFAEEGKLPEHFYFIVKGLVRIYFYLNDKLVIERFEKEEGFFGGSFSHINIRQR
                     LHIYESVEDVTVLRIKYTDLDQLCKVDHTSERVFRIMMEAVHTAYVERLTFFKSLNSE
                     DRYHEFIQKYGDIANRASLKDIANFLEMTPETLSRIRSKYDKR"
     gene            complement(12171..13376)
                     /gene="argG"
                     /locus_tag="PLL59_02090"
     CDS             complement(12171..13376)
                     /gene="argG"
                     /locus_tag="PLL59_02090"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HMY22621.1"
                     /translation="MKDKVVLAYSGGLDTSYCVKYLTEEKNLDVYAITVNTGGFNAAQ
                     LSAIETRAKELGVKEIVIRDVVKEYYEQCIKYLIFGNVLKNNTYPLSVSAERVFQAIA
                     VAEYAKEIGAAYIAHGSTGAGNDQVRFDMVFNIVMPEAKIITPIRDNKLSREEEIAYL
                     KKYGVEIDAKKATYSINQGIWGTSVGGKETLTSRYTLPEEAYPTPLKKDCEPKEISIT
                     FEQGEITALNDKKYEHATDVIIALNEIASAYAIGRDIHVGDTIIGIKGRVGFEAAAPL
                     IIIKAHHLLEKHTLTKQQLFWKDQLSNVYGSNLHEGLFYEPLMRDLEAFMTNTQANVT
                     GKVFIKLQAYRFELVGIESPYDLMSNKFGSYGEMNNAWSGDDVKGFAKIAGNQIMIWK
                     KIKEENKIK"
     gene            complement(13380..14060)
                     /locus_tag="PLL59_02095"
     CDS             complement(13380..14060)
                     /locus_tag="PLL59_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY22622.1"
                     /translation="MKKTSEKFLVQIADSNHISFTQELCHLYEESAKKRGTGIAKRDP
                     EYIKEKILQEKAIIALTSTNKIAGFCYIETWEGKNYVANSGLIVKEEYRHHGLAKRIK
                     KFVFEHTRKKYPNAKIFGITTSLAVMKLNSDLGYKPVTFSELTQDDAFWNGCKSCINY
                     DVLTRTGRKNCLCTGMLYDPKEDKKKEFAKVKKVEKKIPAKLKTPKAQRIKVVKSAGK
                     SKANKVKK"
     gene            14429..15256
                     /gene="lgt"
                     /locus_tag="PLL59_02100"
     CDS             14429..15256
                     /gene="lgt"
                     /locus_tag="PLL59_02100"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMY22623.1"
                     /translation="MNDLLAFIVHWNIKPEIFMITENFGIRWYSLLYGAAFLLGYNIL
                     FWEFEKEGKNTDGADQLLMYMFFAVLIGARLGHVFFYQWDYYKDHLLEIVQIWKGGLA
                     SHGAAICITIALIVYAKLHPEYTFLWIIDRVAIPTAIGGSFIRFGNFFNSEIVGDYTD
                     GSWGVVFERLGETMPRVPIQLFEGFLYVLIFIVLITIYIKKKYKPQEGSLMSIFLIMM
                     FAGRFFLEYWKVDDNPLTMAGITLTHGQWLSFPFIAIAFFIYYLSYKFGKPKETTTA"
     gene            complement(15261..15803)
                     /gene="msrB"
                     /locus_tag="PLL59_02105"
     CDS             complement(15261..15803)
                     /gene="msrB"
                     /locus_tag="PLL59_02105"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMY22624.1"
                     /translation="MKNKKDIVLFLIVVFFLQCQSNPKQTTNSALNQEQKTATMDTST
                     YFKSEEEWKKVLTDEEFHVLREAGTERAYTGKYTDLKDDGNYYCKACGHLLFTSTTKY
                     HSGCGWPAFYDKAGNNAVITRMDYSFGMTRIEVLCKRCESHLGHVFDDGPKDKTGLRY
                     CINSISLTFEKTATDTTKTK"
     gene            15862..17064
                     /locus_tag="PLL59_02110"
     CDS             15862..17064
                     /locus_tag="PLL59_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HMY22625.1"
                     /translation="MKLINSKLCLLLFIFFIGQVIHANEREVKVADLTSSAKVSLLTV
                     GRADHEIWQNFGHTAIRVKDSILGWDLVYNYGTFNFGAPDFMYNFIKGRLLYYESIDN
                     FKDFEQAYREENRSINEQLLNLSQEQKQLIFERLTINAREEYKYYKYDFLYDNCSTRP
                     RNVLFSLFKKVEYAKDKDDDASFRQLIDRNVPNEWLDFGMDLLIGIPTDKKAGYGRTF
                     LPDELMQLCDSAIVDGKPLVISNELILDVIPEYTEKNYITPSLIFWIVFIIVLLLQMK
                     FSFFSRYKIIPVLYFSILGCLGWLFIFMWFFTEHLSTKWNLNILWAMPLNIPLMLFVF
                     REKLPRLVMSYIKFYRILLIMLLIAWWPNPQTYHNAVVPLILLAILFTSLFLQVPTKE
                     DFKKRLFG"
     gene            complement(17068..18279)
                     /locus_tag="PLL59_02115"
     CDS             complement(17068..18279)
                     /locus_tag="PLL59_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMY22626.1"
                     /translation="MESLIGKIKTEYSLYTKEVLAEIGNNKKSLFLGIPKETEYQEKR
                     VPLTPNSVAALVNYGFRISIEKEAGLNSNFSDEEYLNAGADIVFTKADVFKADVILKA
                     GPINQQEINLLNPHQSIFSPILLPNQTKNMVKCMMEKRITAIAFEYVKGDYGTYPFMR
                     SMSEIAGNYAITTAARYLSNEYGKGILLGGIAGQPPAKVMILGAGTVGEAAARSALAL
                     GATVQVFDDNIYRLSRLQHDLGKKIYTSVLDPLNIAKNISRAHVVIGALKPKNGKTPK
                     IVTEDMVRAMKKDSVIIDVSIDQGGCFETSVVTNHDEPIYTKHGVIHYCVPNIASNVS
                     RTASYALSNIIAPILKHAADVGSFENLLKYDEGLRKGAYLYKGALTKQFIAQKFDLKF
                     TDLELLLSADF"
     gene            complement(18283..18744)
                     /gene="tsaE"
                     /locus_tag="PLL59_02120"
     CDS             complement(18283..18744)
                     /gene="tsaE"
                     /locus_tag="PLL59_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HMY22627.1"
                     /translation="MTTPMRFEVNTIDDLDAVSMQITELTASYSIFCFYGEVGAGKTT
                     LIKKICSQLDVIDTISSPTYPIINEYATRNNTIVNHIDLYRLSTIDEALQIGIEEYLD
                     NGNLCFIEWPDNFESLLPTNHIKIFIRKLDTNNRSIEIYNSADVNKTLLKK"
     gene            complement(18757..20313)
                     /locus_tag="PLL59_02125"
     CDS             complement(18757..20313)
                     /locus_tag="PLL59_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HMY22628.1"
                     /translation="MSDIKILWADDEIDLLKPQILFLEQKGYSVEPVSNGHDALDKCK
                     SEHFDLIFLDEHMPGISGLETLSRIKTINKDIPVVMITKNEEENIMEEAIGSQIADYL
                     IKPVKPNQILLAVKKLTENKKLVSEKTATSYRQEFQNIMMELNNVNTHQEWAELYRKL
                     IYWELELEKADNPDMKNILDMQKAEANKEFFKFISKNYLKWLKEPNSDNTPTLSYTLF
                     KNKVFPAIHDDTPIFFLLVDNLRYDHYKVIETLLKEDFRKVSEDYFYSILPTTTQYSR
                     NSIFSGLMPLEIDKKYPEKWFYDDENAGKNLHEEFFLNEQFKRHNKQLKTSYTKITNH
                     KSGKDLEDTILNLMNNHFNTIVYNFVDMLSHARTEMEVLKELASDEAAYRSLTLSWFD
                     HSPLLNALQKLKDKKVRIIITSDHGTMRVQNPSKIIGDKATTTNLRYKTGKNLNFNEK
                     EVFFVKNPEDALLPKSNLSSSYVFAKEDYYFCYPNNYNHFVNYYRNTFQHGGVSIEEV
                     IVPIAIFESK"
     gene            20466..21662
                     /locus_tag="PLL59_02130"
     CDS             20466..21662
                     /locus_tag="PLL59_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMY22629.1"
                     /translation="MNKRKIFNDPVYGFISIPYDIVFDVIEHPYFQRLRRIQQLGLSA
                     LVYPGATHSRFHHSLGAMHLMHRSLEVLKSKGHPISEEESQAAVLAILMHDLGHGPFS
                     HALERVLIENVHHEEISMLMMQQLQLQFGKPLEMAIQIFTNQYPKKFLHQLVSGQLDM
                     DRLDYLNRDSFYSGVSEGVIGYDRIINMLDVHDDNIVVEEKGIYSIEKFIVARRLMYW
                     QVYLHKTSLVADLMLKNAIYRAKFLFANGTLSHSSSSNLDFFLSNAISKNEFALHVDK
                     FAALDDFDIFQALKNWMNVEDKILSQLATSIVQRKLFKIEFLEPAIVQQRYQALQQQY
                     NEEEMQFLVLKGTAENNAYNLTKDNIYIKFKDGSIKEITDIAEQWNIRSLTNAVVKHY
                     IAYPKM"
     gene            21666..22637
                     /gene="lpxD"
                     /locus_tag="PLL59_02135"
     CDS             21666..22637
                     /gene="lpxD"
                     /locus_tag="PLL59_02135"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMY22630.1"
                     /translation="MTIQEIATIIHGDVVGNAHHQIIMPAKIEEATSEHITFLANKKY
                     LDFIANSKAGCVIISKALFSAEMKGNFILVDDAYTAFTKLLSLYVAQQNTEISPQAFI
                     HQSAKIGQQVQLFPFVFIGENVRIGNNCIIYSNVSIYKDCVIGDNCIIHANAVIGSDG
                     FGFAQLSDGSYQKIPQLGNVIIEDDCEIGANTCIDRATLGSTIIRKGVKLDNLIQVAH
                     NVEIGEHTVIAAQTGISGSTKIGHHNRIGGQVGFVGHIHVAPYTQINAQSAVASSVKN
                     EHTAISGTPAIDIKQHFKATVVYKNLPELERTVRNLQAEIEKLKSKD"
     gene            22687..23619
                     /locus_tag="PLL59_02140"
     CDS             22687..23619
                     /locus_tag="PLL59_02140"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HMY22631.1"
                     /translation="MKIQKTITQPIRFSGVGLHTGLMANVVIKAAPENFGIQFCRIDV
                     DPTIYIPAIAANVSNTNRSTTITKYNISVITIEHLMSALYALGIDNALIEIDNKEVPI
                     LDGSAKFYIDNIKSENTIALHQYKKIYTIHDVIEWKDEQQGAEYIFLPHDEFSVTCLI
                     DFPSKMIKHQYAVLNDINDYTNEIATAKTFCFLHEIEFLYANGLIKGGNLNNALVFSE
                     TSLSQEKIKWLASTFHQSANSIPEMGILNPLYQTFDNEAARHKLLDVIGDLALTQTYF
                     KGKLIAYKPGHTSNCKFASYLMKNYVNVTAEHSF"
     gene            23678..24448
                     /gene="lpxA"
                     /locus_tag="PLL59_02145"
     CDS             23678..24448
                     /gene="lpxA"
                     /locus_tag="PLL59_02145"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HMY22632.1"
                     /translation="MTNFIHKDARIANHVEIAHFSTIYEDVEIGENTWIGPNVTVFPG
                     SRIGKNCKIFPGAVIGAVPQDLKFNGEYSLVEIGDNVIVRECATVNRGTAASGTTKIG
                     NHSMLMAYVHVAHDCVIGEHCILANSTNLAGHVTIEDYAYFGGMSGAHQFTRIGKHSF
                     ISGGSMIGKDVPPFCLVMRNPAQYAGINSVGLRRHHFNMEDIHLIQDIYRYIFGNNGL
                     NTTQAIQKIQQEIPESVLRNYIVDFIKTSERGIIKGID"
     gene            24499..25116
                     /locus_tag="PLL59_02150"
     CDS             24499..25116
                     /locus_tag="PLL59_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMY22633.1"
                     /translation="MIIQLENISKRYGFEWIFKKVNCQFEPGFRYAIVGPNGSGKSTL
                     LKVISGGLVPTSGKISYSIPHQRVTDEISAAKEIAYAAPYISLPEDFTLEEFFHFHAS
                     FKKMKVNDVTTFLEIAQLSKHKNKYIKNFSSGMKQRLKLSIAFLTESQVLLLDEPTSN
                     FDHKSVDWYLNMVTEYANDRLLIIGSNQEYEYDFCEKHVSILDYK"
     gene            complement(25120..25710)
                     /locus_tag="PLL59_02155"
     CDS             complement(25120..25710)
                     /locus_tag="PLL59_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22634.1"
                     /translation="MKTSVKFSIILFCIFSLIFSCKKKSLTPNNPDVNSRIIDSVSVV
                     PAGNGRSEAPIDADEDGTTDFYLVSYTDDDAYITSLDAEQVIGDNNFSTDGFNGAAVY
                     NEQAIIDSVSVPKGPRPPKAIWDNLANCSYVAFVGGIEGLAGKGDSYLGFYLEKSDGK
                     HYGWLKLNIPADGKSIKLISIGYHTKPRTAILAGEL"
     gene            complement(25707..25874)
                     /locus_tag="PLL59_02160"
     CDS             complement(25707..25874)
                     /locus_tag="PLL59_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22635.1"
                     /translation="METKEFELPLEGSLSKLALLHQQTTEKPAQIKSQKNKVKNTLHT
                     KSTANFNKKKK"
     gene            complement(25970..27025)
                     /locus_tag="PLL59_02165"
     CDS             complement(25970..27025)
                     /locus_tag="PLL59_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMY22636.1"
                     /translation="MKRITVTFFSVAFCCITFAQSNTQMRYQDLIKWSESIVNSIQSN
                     DIKSIERIANIATLPYDDYCKLFKQTSTPTINIKNSLSAKSLISNDSTTYNQKASDFY
                     FGNLIYSLLNSEQNIAQKNDSIFISIQEENQTIQQSNITKLTLYFINKMEARVLEFPA
                     IIHYNNSFYSISKVAYTTYLNTKETRQYFTPHANSDLVYQYSNNGFVRFPKELENESI
                     DITTSYTEENKVPANSFNTPSSLQNADEVVAFAETMPAFPNGAMGLKRYLKDNLRYPK
                     EASKKGLEGTIYIKFIVDKKGAILSPEVIKDNVGGGCAAEAIRLVSKMPKWWPGKQNG
                     KAVNVYYTLPIKFSIKD"
     gene            complement(27035..29653)
                     /locus_tag="PLL59_02170"
     CDS             complement(27035..29653)
                     /locus_tag="PLL59_02170"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HMY22637.1"
                     /translation="MQELPKNIVYTQIENKWYKHWLDKKYFRSVPDEREPYTIVIPPP
                     NVTGILHMGHMLNDTIQDVLIRRARMQGKNACWVPGTDHASIATEAKVVNLLKEQGIN
                     KSDLTRDQFLEHAWQWKEKYGGIILKQLEKIGASCDWDRTRFTMEADLSDAVIEVFID
                     LYNKGKIYRGVRMVNWDPKGLTAVSDEEVIHKEVNSKLFYIRYKIVDTDDEWITIATT
                     RPETILGDTAICVHPDDERYKHLKGKQALVPLINRSIPIIFDEYIDIEFGTGALKVTP
                     AHDINDYNLGIKHNLEVFDTLNDDGTLSEKAQLFVGEDRFVVRKKIAATLEENGQLQK
                     VEEIKNKVGFSERTDAVIEPKLSMQWFLKMDTLAKPALENVLNDNIKLIPEKFKNTYK
                     HWMENVHDWCISRQLWWGHRIPAWYDEKGNCVVAKTIDEARQIHAAQFPNHNSNNLKQ
                     DDDVLDTWFSSWLWPISVFDGFKNPDGADIKYYYPTNDLVTAPEILFFWVARMIMAGY
                     EYKNEKPFKNVYLTGIVRDKQGRKMSKSLGNSPDLLELIEKYSADGVRTGMLFCSPAG
                     NDLPFDEKLCEQGRNFTNKIWNALRLVKGWTISENHTDNEIVFEWFDNKLELLKENLE
                     KSYASYRLSEALIDLYKFIWDDFCSWYLEFVKPPFGEPIDKKTYDKTISYFEELMKLL
                     HPFMPFISEEIYHHLKEDRTDDICVANYPNNAIYNTNIIHDGEMVKEIISAIRDIRNK
                     NGLSPKVTLSVYVQTNDKRVYEKFGALILKMANTHAIQFVSNDIPNAVAQLIGTDKLF
                     VETGLDIDIESEKKKINEEINYYKGFIESVNKKLQNEKFVTNAKPEIIDNERKKLADG
                     LSKLKSLEDALLALQV"
     gene            complement(29766..30680)
                     /locus_tag="PLL59_02175"
     CDS             complement(29766..30680)
                     /locus_tag="PLL59_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMY22638.1"
                     /translation="MKRKIALLLSVAILFGFRPPKKNENNNQTLELDKALNTNNSLLW
                     QISGNGLTQPSYLFGTIHIISTQDYFLGKNVQKKLENSHELIMEMDLENMDIAALSAI
                     SILDSGKTIKNYLSDSDYQIIQTFMEDSIGVKKFTFEQFYARLKPFYLEQLIYIKYLG
                     QDKESYEDNLKKIADRKKITVSGLETMEEQLQFLEEIPIAVQLKSMIKTIKEYSSETA
                     RLDALIKDYKSENLEVLSKAFEAEEDTIWNDKLLVKRNNNWIPALQMKMEKKSCFIAV
                     GAGHLGGAHGLIALLKAKGYTLEPIASN"
     gene            complement(30748..31311)
                     /locus_tag="PLL59_02180"
     CDS             complement(30748..31311)
                     /locus_tag="PLL59_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HMY22639.1"
                     /translation="MSSFASGPGGPTTKSSTANVSTEGEKVKWLTWDEMVKLNEKNPK
                     NIFIDFYTSWCGWCKVMDRNTFDDPTVAQIMNKYFYSVKFDAERSDTITFLDRKWNFV
                     PSGRKGYHELAAYFMQNELSYPTFCVLTPDFKLITPLKGYIAVPQFEPVISYLGQDFW
                     MPSKNKDIEEYKKSYKSPRTTPWVQPQ"
     gene            complement(31369..31923)
                     /locus_tag="PLL59_02185"
     CDS             complement(31369..31923)
                     /locus_tag="PLL59_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HMY22640.1"
                     /translation="MKKLNFKLLVLMVLSFCFISILYAKNEAAVVVDNQIAWTNIEQA
                     IPQNKISKKKFILINLYTDWCGWCKKMEENTYQDAGIIALMNENFTAAKFNAETTAPI
                     NFNGKTYNFVKTGARGANQLALDLGSVGNKLGYPTIVVLDENGNKLQAFPGFKDVETL
                     SIVLNYFKSGSYKTMDFQQYQSGQ"
     gene            32000..32560
                     /locus_tag="PLL59_02190"
     CDS             32000..32560
                     /locus_tag="PLL59_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HMY22641.1"
                     /translation="MERKIIVAVSGASGSIYTKVLFDKLLHIKQQVSKVGVVMSDNAK
                     DVWKFELGNEDYTSYPFDFYAKNDFMAPFASGSAKYDTMIVCPCSMGTLGRIAGGISN
                     DLTTRAADVMLKERRKLILVTRETPLHLIHINNMKIVTESGGIICPATPSFYSLPNSF
                     EQLAATVIDRVIDLCGFENNSYRWRE"
     gene            complement(32572..34119)
                     /locus_tag="PLL59_02195"
     CDS             complement(32572..34119)
                     /locus_tag="PLL59_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22642.1"
                     /translation="MAKNTAYDIIFILFISIICHLLFSKFGFNPTDEGFVLSASNRLM
                     HGQIPHVDFSSVRPLGYAYLHIPELLFSKTHVFLMSRFIFWLEQVLIAYFWISIIIRY
                     SNTEVTLFLKYTLIIICFLFNVHYFPASVLHTIDGLLLSVLGIYFIISEKKWNYIGYF
                     FIGYAAICKQNYLIILPMTIYLFGRENIIRNMLIGILPIIIYVGYILLNNGWHDLSIQ
                     LSGHNEIVNVGIYSYLSNPLMYIGFISVFVYYKMKINKIYLSILIFSFASILLISNHY
                     HGKFIFLFLGLIAGETTHKIVKQENCILPFIALIIAWSVSISVGYNSPALFAGGMISI
                     LIFMQFNSFKSRIQNKVLLLFVLVGISIFYFVRTNNIYRDMPKKYLRYKLDNIVEGAH
                     GIYTNKNTYSVLLELDSLKKTTQNLVTVPDFTACNILHSHQSKILTEWPNKTEIPNEK
                     ILGKVTSKIKDDTALLFAIPIFQTALLKDGFSPQENKGLSYPIIKFIKENYRKTSFTD
                     YFEIRKK"
     gene            34212..36983
                     /gene="leuS"
                     /locus_tag="PLL59_02200"
     CDS             34212..36983
                     /gene="leuS"
                     /locus_tag="PLL59_02200"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HMY22643.1"
                     /translation="MEYSFSEIEKIARQKWQDNNVYKVTTQSDKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIFSDIYARYKRMKGFNVLHPMGFDAFGLPAEQYAIQTGQHPEITTQK
                     NIETYISQLQKIGFSYDWSRQVSTCEPAYYKWTQWIFLQLFNAYYCTSKQQAFSIANL
                     VRQFEENGNMQNTAYTSADVSPFTALEWNAFSPKQQQDILMEYRLAYQKESFVNWCPA
                     LGTVLANDEIVNGVSERGAHPVEKKKMSQWFLRITAYAERLLQGLETLDWSESMKDMQ
                     RNWIGKSVGAMVSFPLKNFEDKIEVFTTRVDTIFGVSFLVIAPEHELVKQITTDEYKA
                     AVDEYVKVCAAKSDIERQAEKVVSGQFTGAYVLHPFLQTEIPVFIADYVLAGYGTGAV
                     MAVPSGDQRDYLFAKKFELPIIPILDAQQNIEQEADATKEGKYINSDFINGMQYKAAT
                     ALLIEKLEQQKLGYGKINYKMRDAGYSRQRYWGEPFPIYYDNDGIIHTMEDKLPVCLP
                     NVASYKPTGTGESPLANAKDWVTQNGVGTRMETDTMPGYAGSSWYFLRYMDPENPNEL
                     VSKEAQEYWQNVDFYVGGSEHAVGHLLYSRFWHKFLKDIGKVSTEEPFQKMVNQGMIG
                     GRSLLTKKGQIKNVEIALHIPVMLAQQDKLYKNKFQELKDNDNRFENIQIDDIAWETD
                     KDGNDFIALDVQLEKMSKRYLNVVNPDDMVAQYGADTFRMYEMFLGPIDTAKPWDTNG
                     ISGVSNFIRKFWRLFYDELGNAKNIDHKPTQEELKILHKTIKKVNEDIERLAFNTCVS
                     TFMIAVNDLSKLKNVSSVTLLDLIKLIAPFAPHTAEVLWTNLGNNSSVVTCDFPTSDE
                     QYLVESTFNYPIQINGKHRANIEISLETPQEEIQTIVLSDERVTKFTEGKSPKKFIVV
                     QGRIINVVV"
     gene            37189..>39709
                     /locus_tag="PLL59_02205"
     CDS             37189..>39709
                     /locus_tag="PLL59_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HMY22644.1"
                     /translation="MIKKITLTCMLVVAMFIAFAKEHKQAAIDFLQKNLTETKLTSQD
                     IKDLILLDDSYDDFSHINRVWLQQTAYGIPLKNGMLSVHFMNGKVVNYTNNGIQDLKN
                     KISSVTPSITAENAVRFAAAHVGVPSIGKIKLQEKVNKKENLYIFKPIDGLTNLEVHA
                     QLLFTRDKNDKVVLDWEVQFHSLDQYNFWCIDVSAKDGSLIKKTDQVLHCDFGKGEFL
                     GRHTHETEHTSINYRILDENLPSGATDGVSQTSAGSYRVYPFTVESPIYGARQLLVDP
                     HDVVASPYGWHDTNGAAGAEYTYTRGNNVWAYTDKYDADPNTPTSLSTDGGASLVFDF
                     NLDFTTQLDTLTNSKAITTQLFYANNIMHDIFFKYGFNESSRNFQFKNYSGASGGSDL
                     VYAEAHDASLANPRLTNNATFATAPDGTSTPFNRTRMQMYMWDGAPPSSLTYNAPASI
                     AGDIDHGSANGWGPCSYNVTAPVANATSNTAPPSYVCGAVNNPGDIAGKIALIDRGTC
                     NFTEKVYNVQLAGAVGAIIINRQSAGDSLIGMASGTNGGLVTIPAMFVTYADGQKLRD
                     NLATANVTMYRNSTTNCLDLDGSLDFGIVAHEYAHGISTRLTGLSLGTGGTSGCLSNE
                     EQAGEGWGDFFAMALTKKPTDNKNTPRGMGNYAASQDVNGQGIRRYPYSYDMSINPLT
                     YGDLNLNPEKHDIGQVWTSALWDMYWLLVEKHGYSNDLYNGTGGNNRAIKIVVEGLKQ
                     QKCNPGFLDSRNAILKADSILYGYADKCEIWTAFARRGMGASANQGSAGSASDQTEAF
                     DLPAACNTTPTATASFTVSDTTVCVGGSLTFTNTSTASSGSP"
BASE COUNT        12799 a         6574 c         6822 g        13514 t
ORIGIN      
        1 cgtccacgcc aaaaccattt tattttggtg caaagttcgt caataatact ggttctacta
       61 tcgtttctgc tgcagtatca tatagatgtg agcaatggag aagaggaaat tcaacaaacg
      121 gtttaaaaga tacattattt gttgaatact ctacagatgc tgactcagtt cgtttaggta
      181 catggactcg tgttccatcg cttacgggta gttcagtaaa cacaagcact actacaggcg
      241 cattagatgg caatgctgtt ttcactaatg ttgctggaaa tataacagga atggctgttc
      301 caaatggcgc tacattttgg attcgtttta acgactatga tattccaggt tctgaagatt
      361 tattagcagt ggatgatttc agtgtttcat ttacaactgg cactgttgct gcatgtacag
      421 aacctacaaa ctctgtatcg aatttggtgc tatcggcaac aagcactact gccgtaacgg
      481 gctcatttaa tgctacagta ccagcttctg atggctattt agtattgtta gatagcaatg
      541 ctgttacacc aacaattact gatgctacag catattctaa tggtgacctt gttggtactg
      601 cagtagtagt tagtaatggt gcaaacacca cttttaataa aagtggttta gtacaaaata
      661 caatttacta tgcttatgta tttccatata ataatgcagg ttgtttaggt ggtcctaatt
      721 acaaaactag tgcaccagca aatgatagcg ctaaaacatt agttgatgct tgcccagagc
      781 cagcaacaaa tccaactaat atcacattta cattagttgg tgataattca attgaaggtc
      841 aattcaatcg cacaacgcca ccagctgatg gttatattgt tgttcaaagc ttaagttcaa
      901 atattggata tccagctgat agcactaact ataatgttgg tgattctatt gtttctggta
      961 gtttcaaatc aaaagtagct gatgtttcat caagtgccaa tgatactaca tttgttatta
     1021 ctggtttagc atctggcaca aaatacaatg tagctgttat tccatataat ttgtgtggtg
     1081 cattcaaaaa ctatagacga acagcttcta atggtgtaaa cagagacgat acaacaacaa
     1141 caggtacgcc accacttaca gactgtgtgc aaccttcagg tgtgtccaat tcatcaattg
     1201 ttaaattaga ttctactaca tcaaccatta ccattaaatg gaaaaactct tcaaatggtg
     1261 atagtgtaat gattgttgcc gcaccaagtt ctgttggttc catcagtatt cgcgatacga
     1321 tgaactatcc tgtgggcaca attattccat cttcaaatgc tacatcagca aaggtgtatt
     1381 ttagaggaac agatagtaca tgtgtgttga cgggtttatc acaaaatacg ctctacaaaa
     1441 tatttgttat cgcatttaat aacaaaaatt gcttaaatgg acctaactat ggaggcttgg
     1501 caactgcatt tgctaaaacg gcgctctcta caggtgtaaa atacaataat gtggaagcag
     1561 aatttaatct attcccaaat ccgactaaca caggcgcgat atatgttaaa ttcaaatcgc
     1621 caataaaaga agtagcaact ttagaagttg tagatatttt aggtcgcaaa ctgattgcac
     1681 aacaattagc tcctggtaat gatatggaat ctattgatgt aagtagccta tcaaaaggta
     1741 cttatatttt aaatattgtc tataaaggtt caaacaacgt tagcactttt atagtagaat
     1801 aagttaatca ctcaatcata taaacctcgt tttgtttgac tattcaaata aaacgaggtt
     1861 tgtgatttta atagaaaata tgaatagaag atttttttta tactttttac taatatttct
     1921 aataagtccg cttttagtga aggctggtcc tggaaattat tataacattg atagcagtaa
     1981 aagctgtgct gcttttaaat ctatattagc ttctcgatta gcattaggca gcgtttccat
     2041 taattatggt gatgtagatt tttattttaa tagaacagat tcaaagattc aaagccagca
     2101 gaatcaggag gtggtagtgt aattgttgat agatacagcg gtgaacgtcc taatggtctt
     2161 gattcttgta actatcgcta tgatgtagat ttttgttcat caggtggcac tgcttctaca
     2221 caatgtgttt gttatgtaaa agaacattcg tttcctaaat cgtggtttgg aggtaatgtc
     2281 attccaatgt actctgaaat gcatcttttg ttgccagcag ataattatac aaataatgca
     2341 aagagtaatt ttccaattgg atatgtcaaa acaccatcaa ttacttcata taatggaacg
     2401 aaaataggaa gctcagacga ttcattaaac tacggattta atgctacttc agtattcgag
     2461 ccaatcgacc agtttaaagg cgattttgca cgtatttatt tgtatatggt tactcgctat
     2521 gaatctgttg ttgctagttg gatttctaac agtacggcaa atgacgttat ggccggaaat
     2581 agctatcctg ccctcgatcc ttggatactt aaactatgtg tgaaatggca caaacaagat
     2641 ccacctgatt tgttagaacg aaacagaaac gactctgtat ttgtcattca aggcaataga
     2701 aatccatatg tagattatcc gcattgggta gagaaagtat ttggtgtcga tggcatcgat
     2761 acttcctgtg taattacggc tgtacgtaca aattcgaatt cgtttacaag tgctgttttt
     2821 cccaacccag cgaatgatag gttgcaaatt cagacagtat taccttttcc aacaaaagag
     2881 gctagtatca gtgtttttga ttatttagga agatgtattt tatctcataa aataaataat
     2941 ggaactgttg aaaataatac aattaatatt gctagtttgc ctagaggaat ttatttattg
     3001 caaattgaaa acgatggagc aacaagtatg acaaaatttg ttaaagaata aatagccgtt
     3061 ttttctattt tttatgtttc cttttacatc tttttttagt gcattttttc ttctttattt
     3121 ctgtagattt tgtttcagtt gaaatactag tgttacttgt tgatacttct gctttgctag
     3181 tattgacaat ggttttgaca gcttttgtgt ttctaatgta agcatcattt ttccatgaac
     3241 ctactgcata aggcatgcct atctttgatg catagttttt gttgattaat gattgttccg
     3301 taacggtacc gctttctaaa tcacctttga tgttgttggc aatggctatt tgattgccac
     3361 taagcgtaaa caaacctttc aataaaccgc taccagttat ttcgttagca ataaaattca
     3421 tattatcacc actattgtta ttcatcacta cgccataatt ttctattcta tttctgaaga
     3481 aagtattgtc gccaccattg atgccatgtg agttatctat cactaagttt tgaattatat
     3541 ttccttcaaa taaatttgca taagaataat tgccatgcaa aacaatatcg ccagcggcat
     3601 tactcggaaa ccaaccttgc tcccaatatg gatcatacga ataattatag gcgataacat
     3661 tgccgtttgc tgctgcttgc aataaaaatg aatgtcttaa atgcttagca atattatcgt
     3721 aaataaaatt gttatttgcg ccgaattgta aaacgatacc atagcctttt ccaccacttc
     3781 catagtcaaa tgcatcgtgt agataattgc ctgaaatagt agtatttgca gtactttgca
     3841 gtgtgatgtg tgcataattg ctattttcca tttcaactcc ttttacatag caattcactg
     3901 catagttaaa aaatatcgaa gaggtttgtt ggctagtagc atctgttctt tttattttta
     3961 atgcttcaat gccaacttgt tgagctggat ttattttttg tacagtagga gcgttgtttg
     4021 ttgaatatac ataatttaaa ttagcatcaa agctaatttc atttccattt atttttttaa
     4081 tttttaaaat ttgtatgaaa tatggatatg cccaatttga tgtcaaaaca tgtgtcgcat
     4141 tttgtttgac aacaacaaaa tctccaacag caaatttatc agaagatgct actttaattg
     4201 ttgaactatt ctttgttgtg ttagtagtta ttgcagtact tatcggtgtt gtagtacctt
     4261 gcacagatat caaatcgcta ttgccattta gattaaattc caatatagtt ttatcgcttc
     4321 cagcaccacg tagtataata ttcgaaggaa tagaaatact ctttttaaaa agatattttc
     4381 ctgccggaat ttctattata ccataattac cattaaatga ttgaattgca ttttttatcg
     4441 caatggaatt atctgttata ttgtctgctt tagcaccaaa agttatgata gatacttttt
     4501 ttacagatgc tggaaatgta gatgagtgtg caaacgaagt ccactgcaca tctttaattg
     4561 gatacgttgc aaataatagt tgaacacaaa taatttgtag tataaatagc atctttttca
     4621 taatcctttt tttcagtaat gacaaatatt ataccattaa tatcatttat acattcagat
     4681 ttgctgtaag taaaataaat attacctaaa gtttaaaaaa aataccgtaa aggcttgaca
     4741 aattaaaata ttatgtgtaa cattgtattg taaaaccctt agaaccctat tttaaaaaga
     4801 aacaacatag ccaattttct aaaagtttct aattattagt tgagttcaaa gtgtttattg
     4861 ttcatacatg taaaccctta acatgaaacc ttataacaac atcgcattat tgcttttact
     4921 cttattgatg agtggaatta cgtacgcaca aagtgagtta gtagcagaaa atgctagctt
     4981 cacaccaagc aatgcagttg cactttttaa gtctgacgac aaaatcacaa ttagcgaatt
     5041 tgtgcaagat ttaacgaatg ccaatccaac agtaaatgtt aaatgtcaaa ttccattagc
     5101 tgtacaagta aaagtgttta atatggacgg caacatggca aagttagaaa cttatcaatt
     5161 agaaaaagga ttgacagaac ttaatcttaa gcttgatgat ttagcagctg gttcctacat
     5221 ggttcaaatt tattcaaaag aaggcagcgc agttcgacgc ttcgtgaagc ctaattaaga
     5281 tacacgaatt ttagttcgga ttgaattaaa attgatcacc tcgacagttt tcgaaaaacg
     5341 cgcgtaaaat aaattacgtg cgtttttttg tttcaagaat tgattagata ttatctaata
     5401 gcgcattgtc ttcgcccatt agtggcgctg cccagcttgg ttttgctggt gtttcagaga
     5461 atttaaaagg ctgattaata ccttttactt ttccgtagtc agcatgttct acttcaaaaa
     5521 acatgtcacg ttcaattaaa tgtttgtcat tttctacctc ttcgagttct aagataggag
     5581 ttagacatac atctaatggt gctgcgattg cagaccattc atcacgtgtt ttagtaataa
     5641 aaatatcttg taaatctttt tttaattttt caacctcttc tgggattggc atcatacgtg
     5701 gcatccaatc tggtttgcct aacatcatac agacaccttg ccaaaactta ggttctaatg
     5761 agcctaaggc aatccatttt tcatctttac atttatataa atgataattt gccataccac
     5821 cacttagcat gggttcacca cggcgatgtc tgatatttgt atttagtgat tctccaaatt
     5881 gcatagataa caatggcatc acacaatctg tcatggcaac gtcaacaaat tgacctttgc
     5941 cagtgcgttc gcgtgcccat aatgcagata agcaagcaac cactgctgga tagctgccac
     6001 cagccacatc tgctacctga catgctggaa ttataggttt ttctttgtcg ccagttaagc
     6061 ctaatattcc tgaatagcca agataattca tatcatgacc tgccttgtct ttataagctc
     6121 cagaatagcc ataacctgta acagcgacat aaattatttt gtcgttgtgt atttttgccg
     6181 cagcataatc aatgcccatt ttttgtaaaa caccaggtcg aaatgagtcc acaacgatat
     6241 cggctgtttt gaccaattta aagaattttt ctttcccttc atcagatttt aaatctaagg
     6301 atacagatag cttactacgg ttaatactta tataatttaa tgattgtgag cctttcaaag
     6361 gtggaaaaaa tcgagtgtaa tctggtgctt tgggatcttc aattttgatt acttctgcac
     6421 ctaagtcagc caacatttgt gttgctaatg ggccaggtaa taacctgctt aagtctaaaa
     6481 ttcttattcc ttgtaagggt ccttgttgtg gttttcttga catcttttta taattattta
     6541 cacaatgtac gatgtacatt ccttttttaa tattgaaatt tgattgtaac taactattta
     6601 actaaagtta attatacgag actatagaat tgttgataat gataacgtcg aattcagagt
     6661 atgctattta tttttttcta tcagcaaatc caaatacttt tttcaataat tctgttgtgc
     6721 gcgaaagtgg atttgctctt atattatttt cttctttttc tatcattttg aaaacacctt
     6781 caagcgcttt atccgttaca aagcctgtta aatcagtatt tacaggcttt acaaatggaa
     6841 ttttattata aacagtagtt gcgtcagacc agtattttgt ggcgtttact tgccccaatg
     6901 attgatctac aataggtctg aatttttctg ttagaatagg agttgccgtt tttttcaaat
     6961 aggcagttcc tgcaccattg ccaccagtga taatttgtat cgcatcttga acagtcattt
     7021 gttttaatac ttgtaaaaat acatctattg cgacgcctga agctttttct gcagcacgat
     7081 tgagtgatgt agttacgtta tctacttgct tttgcatgcc taatttcaac atagcatctt
     7141 tcactttttc tgcttcagaa ggccatggta ttttcactaa atcattacct agaaatccat
     7201 ctgtggcaga aagcaagctc atacctttcg taatgccgat acctaaggcg tcttttaatc
     7261 catcgccagc ttctttttcg ctaggatcac cataagtagt gcctaatacg gcgcttactg
     7321 tttcttgtat ttgttgtgtt gtacaaccga atgaaaatat agaaacgaca ataattatcc
     7381 agaatctttt catatgcaat ttttaaactt tattctttaa tcgttgtcaa gataataatt
     7441 atattgcaaa ataaatacca aagtatgaag tgtacaattc tgaatattgg cgatgagtta
     7501 ttgataggac agaccttaaa cactaatgct gcttggatgt cgcaaaaaat gaatgaaata
     7561 ggcatagatg taattcatca tatatctctt agtgatgaac gaaatgatat tgaaaattgt
     7621 ttgaacaatg cactcaacga ctctacaatt gtgttgataa cgggtggctt agggccaact
     7681 tcagatgata tcaccaagca agtattatgt gatttttttg gagggaaatt agtattcaat
     7741 gacgctgcat acaaaaatat tgaacgcatt ttcgaacata gaaaaaaact aattgacgag
     7801 gcgacaaaac aagttgcttt tttgccggat aattgtttga ttattcctaa caataatggc
     7861 acagctgcag gcatgatatt taataaagat ggaaaaacta ttgtatctat gccaggtgtt
     7921 cctcatgaaa tgaaagccat gttagatgaa gcactgattc cttacttaaa acaaacctat
     7981 cagttgcctt tcattttaca ttgtaatatt ttaactgcag gtgttggcga aacacaatta
     8041 gcaagcagac ttgagcaatt tgaaaaaaat aaacattcag aaataaaact agcctattta
     8101 ccaagcatag gaaaagttcg attgcgatta acaattaaag gcaatgacaa gttgattttg
     8161 gagcagcatt tatcacaggc gaaatcagaa gtatgccatg ttattgatga ttatatttat
     8221 ggctttgaag atgatacatt agaacaaaaa attggagctt tattgctgga aaaaaaatat
     8281 acacttgcca cagcagaaag ctgtacaggt ggttacctcg cgcatttaat cacttctgtt
     8341 gcaggtagtt catcttatta taaaggaagc attatttctt atgctaacga aacaaaagaa
     8401 aatttattac aggtacacaa aaacacatta ctcgaatatg gagcagttag caagcaaacc
     8461 gttaacgaaa tgcttgctgg tgcattgcaa caattaaaaa ctgatgttgc cattgcaatt
     8521 tctggaattg ctggtcctga tggtggcaca acagaaaaac ctgtaggtac tgtatttatt
     8581 ggtgttggta cacatgagca aaaaattatt aaacgatttg cattaagcag tcatagagat
     8641 agaaatatac aagcagcagg tattttcgct ttggaaatgc ttagaaaatt tttgctgaac
     8701 aaattaaatg agtaagtttg tataggaata gcatgtaaat taattactta ttttaaaact
     8761 aaaattttgt acaatggctg aatataaatt attaatgcca aaaatgggcg aaagtgtaat
     8821 agaagcaact attttagcat ggcataaaaa tgttggagat aaaattaatg agcatgatgt
     8881 tattttagaa gtagctacag acaaagtaga ttctgaagtg cctagtccag taaatggtgt
     8941 gttaaaacaa attctgcacg atatcgatgt ggcagttggt gtaggtgaac cacttgcaat
     9001 aattgtagtt gatggtgatg cacctgctca atatttacct gaaaaaacaa agacagaaac
     9061 tgcatcacaa aaagaattac caattgtcaa agaggaaata attattgaaa atccaacacc
     9121 tgtcaatatt gcagaaacaa aacaagagct tgttcaacaa attgaaaaaa ctgttgcgac
     9181 agcaataaca caaacgattg catcaacaag tgcaaacaca aacaaatact attcgccatt
     9241 agtattgaat attgcacaag tagaaaatat ttctatgcaa gaattagata cgattcaagg
     9301 ctcaggtatt gacaacagag tgacaaaaga agatatttta tcttatattg aattacgtaa
     9361 gcataaacca acaccaaaag tagagacgac tacgtcagtt gctacgcaac aaaaatcatc
     9421 aactccaatt tcagataaaa aagaagagaa atctactgtt gcaaatacgg atagcacaga
     9481 tttatcttca aatattgaaa ttatagaaat ggatagaatg cgcaagttga ttgcacaaca
     9541 catgaccaat tctagaaaaa cagcagcaca tgtttcctca tttgtagaat gcgatgtgac
     9601 gaatattgtc agatggcgcg aaaaaaataa aaatgcattc caaaaaagag aaggttttag
     9661 cttgtcgttt atgcccatat ttgtcgaagc aattacaaaa gctataaaag attttccgat
     9721 gataaatgtg agtgtagatg gcgacaaaat cattaagaaa aaagacatca acgttggcat
     9781 tgcaacagct ttaccaaatg gtaatttgat tgtgccagta gtgaaacaag ctgatcaaaa
     9841 aagcttgtta ggaattgcta aagcgatgaa tgaaattgtg ttgaaagctc ggaaaaatgc
     9901 actacaacct gacgatgtga tggacggcac ttataccatc tccaacattg gtacatttgg
     9961 aaatatcatc ggcacaccta tcattaacca gcctcaggtg gctattatgg ctgttggtgc
    10021 aataacaaaa aaaccagttg ttgtcgaact cgatggacaa gatgtaattg caattcgaca
    10081 catgatgtat ttgtctcata catatgatca tagagtagta gatggtcaat taggaggctt
    10141 atttgtaaaa aaagtagcag attatttaga aaaatttgat ttacatcagg aaatttaata
    10201 aattgctgaa cgaaaccata caatgaagaa aaaattagat ataaaggaat gtaaagaaat
    10261 attgtataat gtgttgaaac aatatagcaa tcttgaagat gttattttac acgaaatggt
    10321 tgcgaatttt gaattagaag tatttcccaa aaattcagta atcattccaa aaaattgcac
    10381 gaataataaa ttattttttg tgttaaatgg tttagtacgt atttactatg aagcagaagg
    10441 aaaagaaatc acctatgatt ttaaagaaga aaattcattt tttatcaatg gttataccat
    10501 ctttacgaag cttcctaatt ttgatttaca tgtagcgatt gagcatactg tttgcttaag
    10561 tgcaaactat gacaaaatgg aagcaatcat ggaaaagtac catcaagttg aacatttagg
    10621 cagaaagatg gttgaactat attatgcaaa ctatttgatt gcaaactaca ataaaatgtt
    10681 tttgtcagct gtagagcgtt acgaattatt tgcaaaagat tatagcagtt tgatgttgcg
    10741 tttgccgttg cgctttgtag catcatattt aggaatagca ccagaaacat taagcagatt
    10801 acgctccaaa tactaaagga aacatgaaaa ggaaaccaat aaatattgaa gaacacagag
    10861 ggaaattatt cagccttttt atgcgtttgg gaccaaaaac acctgcgaat gaattacaag
    10921 aattagtaga tcagtttgag attggtatgt tttctaagcg cgaaatagtc ttacaagcag
    10981 gtgagttttc tgataccgtt tattttattg tgagtggctt ggtacgcata tattatgtca
    11041 aagaagaaaa agaaattact aattggttta tcaaagaaaa tatgatgttt gcagcaacct
    11101 attctatact cactggtaaa gcaaattata gtagttacga agccttggaa gatacagtag
    11161 tattaaaaat aaactattcc actttagaaa agtattatgc aaaatatcac agtattgaac
    11221 acattggcag gaaattaatt gaatactatt acggtacatt tatgaaaaaa actttcgacg
    11281 ttttattcct ttcggcagaa gaacgttata gtttatttat gaaagaccat ggcgatttga
    11341 ttaatcgtgt tcctttgcga tttattgcat cgtatttagg cattacacaa gaaacattaa
    11401 gcagacttcg ttcgaaataa ttaatcttca tttttttttg ccgtattaat tttcgaaaat
    11461 acattttctt ttcaaaatta ataattagtt gacatggtat cattcatcaa atgtagcatg
    11521 tgtcattttt tttaataaat ttgacagtac gtaaacatga catctcaagc ccacaactta
    11581 caagaaaaga atgcaagagc attactcagg caaatattgt ctggcactgc acatgttgat
    11641 tcaaataaat tagatgcatt agtaaatttg tttgaaatgg tttcctacaa gcgaaatcat
    11701 aaatttgccg aagaaggtaa attgccagag catttttatt ttatcgtaaa aggattagta
    11761 cgaatttatt tttatttaaa cgataaattg gtgattgagc gctttgaaaa agaagaagga
    11821 tttttcggag gcagtttttc gcatatcaat attcgccaaa gattacatat ttacgaatct
    11881 gtggaagacg taactgtgtt gcgcataaaa tatacagatt tagatcagtt gtgcaaagtt
    11941 gatcatacat cagagcgcgt gtttagaata atgatggaag ctgtgcatac agcatacgta
    12001 gaacgtttga cgtttttcaa gtctctaaat tctgaagata gataccacga atttattcag
    12061 aagtatggtg atatcgccaa tagagcatct ttaaaagata tcgcaaattt tttagaaatg
    12121 acgccagaaa cactaagtcg tattcgctca aaatacgaca agcgataaac ttatttaatt
    12181 ttattttctt ctttaatttt tttccaaatc attatctgat tgccagcaat ttttgcaaag
    12241 cctttcacat cgtcgccact ccaagcattg ttcatctcgc cataacttcc aaacttatta
    12301 ctcatcaaat catacggcga ctcgatgcct actaattcaa aacgatatgc ctgtagtttg
    12361 ataaatactt ttcctgtaac attcgcttgc gtatttgtca taaatgcttc taaatcgcgc
    12421 attaaaggtt catagaataa accttcatgt aaattgctac catacacatt gctgagttgg
    12481 tctttccaaa aaagttgttg ctttgtaagt gtatgctttt ctaatagatg atgcgcttta
    12541 ataataatta aaggtgcagc cgcttcaaat ccaactctac cttttatgcc gataatggta
    12601 tcgccaacat gtatatctcg accaatggca tatgcactcg ctatttcatt gagtgctata
    12661 ataacatctg ttgcatgttc gtatttttta tcattaagtg cagtgatttc gccttgttca
    12721 aaagtgatgc tgatttcttt gggctcgcaa tcctttttta atggtgtagg atatgcctct
    12781 tcaggcaatg tatatcgtga tgttaaagtc tcttttccac caacgcttgt tccccaaatt
    12841 ccttgattta tggaatacgt tgctttcttt gcatcgattt ctacaccata tttttttaaa
    12901 taagcaattt cttcctcgcg cgataattta ttatctctaa taggtgtaat tatttttgct
    12961 tcaggcataa caatattgaa caccatgtca aaacgcactt ggtcatttcc tgcgccagtg
    13021 ctaccatgcg cgatgtatgc agcgccaatt tctttggcat attctgcaac agctattgct
    13081 tgaaaaacac gctctgcaga aacggataat ggataagtat tgttttttaa tacattgccg
    13141 aatattaaat atttaataca ttgttcgtag tattctttaa ccacatcgcg aattactatt
    13201 tcttttacgc caagttcttt agcacgtgtt tctattgcac ttaattgcgc tgcattaaat
    13261 ccgcctgtgt tgacagtgat agcataaaca tctaaatttt tttcttctgt aagatatttt
    13321 acacaatacg atgtgtctaa accaccacta tatgctaata caactttatc tttcatgatt
    13381 tattttttta ctttatttgc tttgcttttg ccagcagatt tgaccacttt aatgcgctgt
    13441 gctttcggag ttttcaattt cgcaggaatt ttcttttcta cctttttgac tttggcaaat
    13501 tcttttttct tgtcttcctt cggatcgtaa agcatacctg tgcacaaaca attcttcctt
    13561 cctgtgcgtg tgagaacgtc atagtttata cagcttttgc acccattcca aaatgcatca
    13621 tcttgtgtga gctcagaaaa agtaacaggt ttgtagccta aatctgaatt taatttcatc
    13681 acagctaagc ttgtcgtaat gccaaatatt ttcgcatttg gatatttctt tcttgtatgt
    13741 tcgaaaacaa actttttaat gcgttttgct aaaccatgat gtctgtattc ttctttgaca
    13801 attaagccag agttggcaac atagtttttt ccttcccaag tttcgatata acaaaatcca
    13861 gcaattttat ttgtcgatgt caatgcaata attgcttttt cttgtaatat tttttctttg
    13921 atgtactcag gatcgcgttt tgcaattcct gtacctcttt tttttgccga ttcttcatat
    13981 aaatgacata gctcttgcgt aaagctgata tgattgctgt cggcaatttg aaccaaaaat
    14041 ttttcggaag tttttttcaa ttttatgacg tgcttaaaga caaaaatcta acctaacaaa
    14101 aatatagagc attttgtaaa ttgaatgtta ttttttaatg tgaatctact ttgttttgtt
    14161 agcattcatt taaaatactg ccactatttg ctgaaaattt tagattattt tagattattt
    14221 tagtttattg aagaaaagaa aggttttatt ttgtgcttat gcaaattttt tttctaaaag
    14281 tttgtatcta tatagttagt ctatttgctt ctttgctaaa catagacatc acatcattgc
    14341 attttttaat tttcttaact tttgaagcac acactatgca ttaggctaaa taaaataaat
    14401 tactaacttt ggcgcacaaa taaagtatat gaacgattta ttagcattta ttgtacactg
    14461 gaatattaaa ccagagatat ttatgattac agagaatttc ggtattcgtt ggtatagttt
    14521 attatatggt gcagcatttt tattagggta taatattttg ttttgggaat ttgaaaaaga
    14581 aggaaaaaac acagatggtg cagatcagtt gctgatgtat atgttctttg ccgtgttaat
    14641 aggcgcgcgc ttagggcatg tttttttcta tcaatgggat tattataaag atcatttgct
    14701 ggaaattgtg caaatatgga aaggtggttt agcaagtcat ggcgctgcca tttgtatcac
    14761 tatcgcctta atagtttatg caaaacttca tccagaatat actttccttt ggataataga
    14821 tagagtagct attccaaccg ctatcggtgg ttcttttata cgttttggta attttttcaa
    14881 ttctgaaatc gttggcgatt atacagatgg aagttggggc gttgtgttcg agcgattagg
    14941 cgaaacaatg ccacgtgttc cgattcagct ttttgaagga tttttatatg tgcttatttt
    15001 tattgtgtta atcaccattt atatcaagaa aaaatacaaa cctcaagaag gcagcttaat
    15061 gtctatattt ttaattatga tgtttgctgg tcgtttcttc ttggaatatt ggaaagttga
    15121 tgacaatcca cttaccatgg caggcatcac actcacgcac ggacagtggt tgagttttcc
    15181 atttattgct attgctttct ttatctatta tttgtcttac aaatttggca agccaaaaga
    15241 aactaccact gcttagtttt tcattttgtt tttgtcgtgt ctgtggctgt tttctcaaat
    15301 gtcaatgaaa tagaattgat gcaataacga agtcctgttt tatcttttgg gccatcatcg
    15361 aatacatgtc ctaaatgaga ttcgcatctt ttacataata cttcaatacg cgtcattcca
    15421 aagctataat ccatgcgtgt gataacagca ttgtttccag ctttatcata aaacgctggc
    15481 caaccacaac cactatgata ttttgttgtc gacgtaaata ataaatggcc acaagctttg
    15541 caatagtaat tgccatcatc tttcaaatca gtatattttc cagtgtatgc acgttcggtg
    15601 ccagcttcac ggagtacgtg aaattcttca tcagtcaata cttttttcca ttcctcttcg
    15661 cttttgaaat aagttgacgt atccatcgtt gcagtttttt gttcttgatt taatgcagaa
    15721 tttgtagtct gttttggatt cgattggcat tgaagaaaaa agacaacaat taaaaataac
    15781 acaatatcct ttttattttt cataacacta tattacataa actgttttct gaataaaaag
    15841 ttttatctct atcttcgcaa tatgaagtta atcaatagta agctgtgttt gttgcttttc
    15901 atttttttta ttggacaagt cattcatgcc aatgaaagag aagtaaaagt agccgacctt
    15961 acatcttcgg caaaagtgag tttgctgaca gttggtagag cagaccacga gatttggcaa
    16021 aactttggac atacagccat tcgtgtaaag gattctattt taggctggga tttagtctat
    16081 aattatggaa catttaattt cggcgcgcct gattttatgt ataacttcat taaaggaaga
    16141 ttattgtatt acgagtctat cgataatttt aaagattttg aacaagcgta tagagaagaa
    16201 aatcgctcga ttaacgaaca attattaaac ctgtcgcaag aacaaaaaca attaatattc
    16261 gaacgattaa cgattaacgc gcgcgaagaa tataaatatt acaagtacga tttcttatat
    16321 gataattgct ctactcgacc aagaaatgtt ttgttttcat tgtttaaaaa agtcgaatat
    16381 gcaaaggata aagacgacga tgccagcttt agacaattga tagatagaaa tgttcccaat
    16441 gaatggctcg actttggaat ggatttatta attggcattc caaccgataa aaaagctggc
    16501 tatggaagaa cttttttgcc tgatgaatta atgcagttat gcgatagcgc tatagttgat
    16561 ggaaaacctt tagtgattag caatgaattg attttagatg tcattcctga atacacagaa
    16621 aaaaactata taacaccaag tttgatattc tggattgtgt ttattatagt attgctgttg
    16681 caaatgaaat tttcattttt tagtagatat aaaatcattc cagttttata tttttctata
    16741 ttaggatgct taggttggct ttttattttt atgtggtttt ttacagagca tttatctaca
    16801 aaatggaatt tgaatatact ttgggcaatg ccgttaaata ttccgttgat gctgtttgta
    16861 ttcagagaaa aattaccacg actagtaatg tcatacataa aattttatag aatactgttg
    16921 attatgttgc ttatcgcttg gtggcccaat ccacaaacat atcacaatgc tgttgtgcca
    16981 ttgattttat tggctatatt atttacttcc ttatttttac aagtgccaac aaaagaagat
    17041 tttaagaaaa gattattcgg ctaaaaatta aaaatcagcg ctgagtagta attctaaatc
    17101 agtgaatttt aaatcaaatt tttgtgcaat aaattgcttc gttaatgcgc ctttatagag
    17161 gtatgcgcct ttgcgtaagc cttcatcata ttttaataag ttttcaaaac tgcctacatc
    17221 tgcagcatgc tttaaaatag gagcgatgat gttgcttaaa gcatagcttg ctgttctcga
    17281 aacattagaa gcaatatttg gcacgcaata atgaataacg ccgtgtttgg tataaattgg
    17341 ctcatcgtga ttagtaacaa cagatgtttc aaaacaaccg ccttggtcga tggaaacatc
    17401 aatgattacg gagtctttct tcatggcgcg caccatgtct tccgtaacaa tttttggcgt
    17461 ttttccgttt ttaggtttta atgcaccaat gacaacatgt gctcttgata tattttttgc
    17521 aatatttaat gggtctaaaa cagaagtgta aattttcttt cctaaatcgt gttgcaatct
    17581 ggataaacga taaatattat cgtcaaatac ttgaacggtt gcgcctaatg ctaatgcaga
    17641 tcttgctgct gcttcaccta ctgtgccagc gcctaaaatc attactttcg caggtggttg
    17701 tccagcaatg ccacctaata aaataccttt tccatattca ttacttaaat agcgcgcagc
    17761 agtggtaatg gcataatttc cggcaatctc gctcatgctt cgcataaatg gataagtgcc
    17821 gtaatcgcct tttacatact caaaggcaat agcagtaatg cgtttttcca tcatgcattt
    17881 taccatgttt tttgtctgat taggcaataa aataggagag aatatagatt gatgtggatt
    17941 taataaattg atttcttgct ggtttattgg tcctgctttt aatattacat cagccttaaa
    18001 aacatcagct tttgtaaata caatatcagc acctgcattt aaatattctt cgtcgctaaa
    18061 gtttgaattt aatccagcct ctttttcaat gctaatacga aaaccataat tcactaatgc
    18121 tgcaacagag tttggtgtta gtggtactcg cttttcttga tattcagttt cttttggaat
    18181 gccaagaaat aatgactttt tgttattgcc gatttctgcc aacacttctt tggtgtataa
    18241 agaatattcc gttttaattt ttcctattaa agattccatg tattatttct tcagcaaagt
    18301 tttgttgaca tcagcagaat tataaatttc aatacttctg ttattggtat ctaatttacg
    18361 aataaaaatc ttaatatggt ttgtaggtaa caaactctca aaattatcag gccattcgat
    18421 aaaacataaa ttgccattat ctaagtattc ttcaatgcct atttgtagtg cttcatcaat
    18481 ggtggaaagc ctatataaat ctatatgatt gacaatggtg ttattgcgtg tagcatactc
    18541 attaatgatt ggataagtcg gtgaagatat cgtgtctatg acatcgagtt gcgaacaaat
    18601 tttttttatt aatgtcgttt ttcctgcacc tacttcacca taaaaacaaa atatagaata
    18661 gcttgcagtg agctctgtaa tttgcatact tacagcatct aaatcgtcaa ttgtatttac
    18721 ttcaaaacgc attggcgttg tcatggaaat tgaaatttat ttactttcaa atatggcaat
    18781 tggtactatt acttcctcta tagaaacacc gccgtgttgg aatgtatttc tataataatt
    18841 gacaaaatga ttgtagttgt ttggataaca aaagtaataa tcttctttcg caaatacgta
    18901 cgaagaactc aaattagatt tcggaagtaa agcgtcttcc ggatttttga caaaaaatac
    18961 ttccttctca ttaaagttta gattcttgcc agttttgtat cgtaagtttg tagtagtggc
    19021 tttgtcgcca attatttttg aaggattttg tacacgcatg gtgccatggt cagaggtgat
    19081 aatgattctc acctttttgt cttttaattt ttgtaaagcg tttaataatg gcgaatggtc
    19141 gaaccacgat aatgtcagag aacggtaggc agcttcgtcg cttgctagct cttttaatac
    19201 ttccatttca gtacgtgcat gtgataacat atctacaaaa ttatagacaa tagtattgaa
    19261 atgattattc attaaattca gtatcgtgtc ctccaaatct ttgccacttt tatggttggt
    19321 gattttagta taacttgttt ttaactgttt gttatgtcgt ttaaattgtt catttaagaa
    19381 aaattcttca tgtaaatttt tgccagcatt ctcatcatca taaaaccatt tttcaggata
    19441 ttttttatct atttctaatg gcattaatcc agagaaaatt gaatttctag agtattgcgt
    19501 tgtcgttggt aaaatactat aaaaataatc ttcacttact tttctaaaat cctctttcaa
    19561 caatgtttca ataactttgt agtggtcgta tctgagattg tcaactaaaa gaaagaaaat
    19621 aggcgtgtca tcgtgaattg ctggaaatac tttattctta aataatgtgt atgataaagt
    19681 tggcgtattg tcgctgttgg gttctttcag ccacttcaaa taattttttg aaataaattt
    19741 gaaaaactct ttattggctt ctgctttctg catgtccaag atatttttca tgtctggatt
    19801 gtcggctttt tccaattcca attcccagta aatcaacttt cgatataatt ccgcccattc
    19861 ttgatgtgtg tttacattat tcaactccat catgatattc tgaaactctt gacgatagga
    19921 ggtcgctgtt ttttcggaaa ctaatttttt attttctgtt aattttttaa cggctaataa
    19981 aatttgattt ggctttacag gttttataag ataatccgca atttgtgaac caatcgcttc
    20041 ttccataatg ttttcttctt catttttggt tatcatcaca actggtatat ctttgtttat
    20101 ggtttttatg cgtgacaagg tttccaatcc agaaatacca ggcatatgtt catctaaaaa
    20161 tattaaatca aaatgttctg acttgcattt atctaaagcg tcatggccat tgctgactgg
    20221 ctcgacggaa tagcctttct gttcaagaaa tagaatttgt ggttttaata aatctatttc
    20281 atcatctgcc caaagtattt ttatatcact catctgcgtt gttgttgtat gattagaagt
    20341 aattagtttt atgtattttg tgctgtaatt acttatttta tacgcaaatt acaaatttaa
    20401 ttctattaat aaatgtgaat tagtgtaaaa tgaagtagca aatttatacg tatattcatt
    20461 gaaacatgaa caaacgaaaa atcttcaacg atcctgtgta tggttttata tcaatacctt
    20521 atgatattgt atttgatgtt atagaacatc cttattttca gcgtttgcga cgcattcaac
    20581 aacttggcct tagtgctttg gtttatccag gagctacaca ctcgcgattt catcattcat
    20641 taggtgcaat gcatttaatg cacagaagtt tagaagtcct taaatcaaaa ggacatccaa
    20701 tttctgagga agaatcgcaa gcagcagtat tggccatatt aatgcacgat ttaggtcatg
    20761 gtccgttttc gcatgcacta gaacgtgtgt tgattgaaaa tgtacatcat gaagagattt
    20821 cgatgttgat gatgcagcaa ttgcaactac agtttggtaa gccattagaa atggcgattc
    20881 aaatttttac caatcaatat ccaaagaaat tcttacatca gctggtaagc ggacaattgg
    20941 atatggatag attagattac ttaaatagag attctttcta tagtggcgta agcgaaggtg
    21001 tgattggtta cgatcgcatc atcaatatgt tagacgtgca tgacgataat attgttgtcg
    21061 aagaaaaagg aatttattct atcgaaaaat ttattgttgc acgccgcttg atgtattggc
    21121 aagtgtattt acataaaaca agtttagttg ctgatttgat gcttaaaaat gctatttata
    21181 gagcaaaatt tttatttgca aatggcactt tatcgcattc atcttcttcc aatctagact
    21241 tctttttatc taatgcaatt tcaaaaaatg agtttgctct tcatgtcgat aaatttgcag
    21301 cacttgatga ttttgacatt ttccaagctt taaaaaattg gatgaatgta gaagataaaa
    21361 tattaagcca attagccaca tcaatcgtgc agcgcaaact ttttaaaatt gaatttttag
    21421 aaccagccat tgtccaacag agatatcaag cgttacagca gcagtataat gaggaagaaa
    21481 tgcaattttt ggtgcttaaa ggaacagcag aaaataatgc atataatctg acaaaagaca
    21541 atatttatat taaatttaaa gatggttcga ttaaagaaat cacagatatt gcagagcaat
    21601 ggaatattcg ttcactgaca aatgctgtgg tgaaacatta tattgcgtat ccaaaaatgt
    21661 aaacattgac aatacaagaa atagcaacaa ttattcatgg cgatgtagtc ggaaatgcac
    21721 atcaccaaat cataatgcct gccaaaatag aagaggcaac aagcgagcat attacctttt
    21781 tagcgaataa aaaatatctt gattttattg ccaattcaaa agctggttgt gtcattattt
    21841 cgaaggcact tttttcggct gaaatgaaag gtaattttat ccttgttgat gatgcatata
    21901 cagcatttac caagcttttg tctttatatg ttgcacaaca aaatacagaa atttcaccac
    21961 aagcatttat ccatcaaagt gctaaaattg gtcaacaggt acagttattt ccgtttgtat
    22021 ttataggcga aaatgttcgc atcggaaata attgtatcat ttattctaat gtaagtattt
    22081 ataaagattg tgtaatcggc gataattgta taatacacgc aaatgcagtc attggtagcg
    22141 atggctttgg ctttgcccaa ttatcagatg gttcctacca aaaaattcca caattaggaa
    22201 atgtcatcat cgaagatgat tgtgaaatag gcgcaaacac ttgtattgac agagcaactt
    22261 tgggctcaac aataattcga aaaggagtga agctagataa tcttattcag gttgcacata
    22321 atgtagaaat tggcgaacat actgtaattg ctgctcaaac cggaatttct ggcagtacta
    22381 aaattggcca tcataataga attggtggac aagtaggttt tgttggtcat attcatgttg
    22441 caccatacac acaaatcaat gcacaaagtg cagtggcatc gtctgtcaaa aacgaacaca
    22501 ccgcaatttc aggtacacca gctatcgata tcaaacagca ttttaaagca acagtcgtct
    22561 ataaaaattt gcctgaatta gaaagaacag ttaggaatct tcaagcagaa atcgaaaaat
    22621 taaagtcaaa agattaatgt ttattggcat tttcgtatat tcgtatttag tttaaatatt
    22681 tccatattga aaatacaaaa aacgataaca cagccaattc gattttcagg cgttggatta
    22741 cacactggat taatggcaaa cgttgtgata aaagcagcgc cagaaaattt tggaattcaa
    22801 ttttgtagaa tagacgtcga tccaactata tatattcctg ccatagctgc taatgttagc
    22861 aataccaaca gaagcacaac catcactaaa tacaatattt cggtaataac tatcgaacat
    22921 ttgatgtcag cattatacgc tttaggcatc gacaatgcat taatagaaat tgacaataaa
    22981 gaagtaccaa ttttagatgg aagcgccaag ttttatatcg acaacatcaa atcagaaaac
    23041 accattgcat tacatcaata taaaaaaata tacacgattc acgatgtgat agaatggaaa
    23101 gatgaacaac aaggtgcaga atatattttt cttccacatg atgaattttc agtgacttgt
    23161 ctcatcgatt ttccttctaa gatgattaaa catcaatatg ctgtattaaa cgatattaat
    23221 gattatacaa acgaaattgc aacagcaaaa acattttgct ttttacatga aatcgaattt
    23281 ctatatgcta atggtttaat caaaggtggc aacttaaaca atgcattggt tttttcagaa
    23341 acatcattaa gccaagaaaa aattaaatgg ctggcttcaa cttttcatca atcagcaaat
    23401 tcaattcctg aaatgggaat tttaaatcct ttatatcaga cctttgacaa tgaagcggcg
    23461 cgtcacaaat tattagatgt tatcggcgac ttagcgttaa cacaaaccta ttttaaaggt
    23521 aaactaattg cttacaaacc aggacacaca tcaaattgta agtttgcaag ctacttgatg
    23581 aaaaattatg tcaatgttac agccgaacat tcattttagc aattaatgta tatagtacaa
    23641 tctctttccc ttttgtatat ttgcataaat atcttttttg accaatttta ttcataaaga
    23701 cgctcgcatt gctaatcatg ttgaaatagc tcatttctca acaatttacg aagatgttga
    23761 aattggcgaa aatacttgga taggaccaaa tgtaaccgtg tttccaggca gtagaattgg
    23821 taagaactgt aaaatttttc ctggcgccgt tattggcgca gtgccgcaag atttaaaatt
    23881 taatggcgaa tattcattag tcgaaattgg agataatgtg attgtgcgcg aatgtgccac
    23941 tgtcaataga ggcactgctg caagtggcac tacaaaaatt ggcaatcact cgatgctaat
    24001 ggcttatgta catgtagcgc atgattgtgt gattggtgag cattgtattt tggcgaattc
    24061 aacaaactta gctggccacg tgacaataga agattatgcc tattttggtg gcatgagtgg
    24121 cgcacaccaa tttacgcgta tcggaaaaca tagttttatt agcggtggaa gcatgatcgg
    24181 caaagatgta ccaccatttt gtttggtgat gcgaaatcca gcgcaatatg caggcattaa
    24241 ttctgttggt ttacgtcgcc atcacttcaa catggaagac attcacttga ttcaagatat
    24301 ctatcgttat atttttggaa ataatggttt gaatacgaca caagcgattc aaaaaataca
    24361 acaagaaatt ccagaatctg tgcttaggaa ttatattgta gattttataa aaacatcaga
    24421 acgaggtatt attaaaggca tcgattagct caattattaa acagcatatt tttcattcat
    24481 acaatttcat caataacatt gattatacaa ctcgaaaata ttagtaaacg ctatggcttt
    24541 gaatggattt tcaaaaaagt aaactgccaa tttgagccag gatttcgata tgcaatagtc
    24601 ggcccaaatg gaagtggaaa atcaacatta ctaaaagtga tttctggcgg tttagtgcct
    24661 acatcaggta aaattagtta tagcattcca catcaaagag tgacagacga aatttctgca
    24721 gcaaaagaaa ttgcctatgc ggcaccttat atttcattgc cagaagattt tacattagaa
    24781 gagtttttcc attttcatgc ttctttcaaa aaaatgaaag taaatgatgt tactacattt
    24841 ttagaaattg cacaattaag caaacacaaa aacaaataca tcaaaaattt ctcatcagga
    24901 atgaagcaaa gattgaaact gtctattgca tttcttacag aaagtcaagt tttactctta
    24961 gatgaaccaa cttccaactt cgaccataaa tctgtggatt ggtatttgaa tatggtgaca
    25021 gaatatgcga acgacagact attgattatt ggttctaatc aagaatatga atatgatttt
    25081 tgcgaaaaac acgtttcaat tcttgactat aaataagatt tataattcgc ctgctaagat
    25141 agccgttcgc ggttttgtat gatagccaat cgagattagt tttatgcttt tgccatcagc
    25201 aggaatattt aatttcaacc aaccataatg ttttccatcg cttttttcta agtaaaatcc
    25261 aagataggaa tcacctttgc cagccaatcc ttcgatgccg cctacaaatg ctacataaga
    25321 gcaattggcc aagttgtccc aaatggcttt cggcggtcgt ggtcctttag gcacagacac
    25381 agaatctata atagcttgtt cgttatacac tgctgctccg ttaaatccat cagtcgaaaa
    25441 attattatca cctattactt gctccgcatc taaagatgtt atataggcat cgtcatcagt
    25501 atatgatact aaataaaaat cagtggtgcc gtcttcgtca gcatctatag gcgcttcgct
    25561 tcttccattg ccagcaggaa caacagatac gctgtcgata attctgctgt ttacatcagg
    25621 attgtttggc gttaacgact tctttttaca agaaaatatg agtgaaaaaa tacagaaaag
    25681 gataatgcta aattttacgc ttgttttcat tttttctttt tattaaagtt agcagtagat
    25741 ttcgtatgta aagtattttt tactttgttt ttttgagact tgatttgtgc tggtttttca
    25801 gtagtttgct gatgcaataa tgctaactta ctcaaggagc cttctaaagg tagttcaaat
    25861 tctttggttt ccatagctta ggtttctttt gaaataaaat tatgaaaacc aaggtcaagc
    25921 gcgtgttata cttagtattc ggtgcgttta attgagaatt cgtgagattt taatctttta
    25981 tgctgaattt tataggtaat gtataatata catttacggc tttgccattc tgctttccag
    26041 gccaccattt tggcatttta ctcaccaatc gaatggcctc agcagcacag ccaccgccaa
    26101 cattatcttt tatcacttct ggcgataata ttgcaccttt tttatctaca ataaatttta
    26161 tataaatagt tccttccaat ccttttttcg aggcttcttt aggatagcgc aaattatctt
    26221 tcaaataacg ttttaagccc attgctccat ttggaaaagc aggcatcgtt tctgcaaacg
    26281 ctactacttc atcagcattt tgtaacgatg atggtgtgtt aaaactattt gcaggaactt
    26341 tgttttcttc agtgtatgat gttgttatgt caatagattc attttctaat tctttaggaa
    26401 agcgcacaaa tccattattg ctatattgat atacaagatc agaatttgca tgaggagtaa
    26461 aatattgtct cgtctcttta gtattcaaat aagttgtata agcaacttta ctaatgctgt
    26521 aaaaggaatt gttgtaatga attattgcag gaaattctaa tacacgtgct tccattttat
    26581 ttataaaata gagcgttaat ttcgtgatgt tggattgttg aatggtttga ttttcttctt
    26641 gaatgctgat aaaaatggaa tcattttttt gcgctatatt ttgctcgcta tttaacaagg
    26701 agtaaattaa attgccaaaa taaaaatcac ttgctttctg gttgtatgtt gtcgaatcat
    26761 tgctaattaa actctttgca gataaactat ttttgatatt aatagtcgga gttgatgttt
    26821 gtttaaataa tttgcaatag tcatcatacg gcaatgttgc aatatttgca attctctcaa
    26881 tactttttat gtcattggat tgtatgctgt tgacgattga ttcgctccat ttaatcaaat
    26941 cttgataacg catttgtgtg tttgattgtg caaatgtgat gcagcaaaat gctacactaa
    27001 aaaatgtaac cgttattctt ttcatgtgca tgaattagac ttgtaaagca agcagcgcat
    27061 cttccaatga ttttaatttt gagagaccat ctgccaattt ttttctttca ttatcgatga
    27121 tttctggttt tgcattcgtg acaaattttt cgttttgtag ctttttattt acactttcaa
    27181 taaaaccttt atagtaattt atttcttcgt ttattttttt cttttctgat tcaatgtcaa
    27241 tgtcaaggcc tgtttcaaca aataatttat ctgtgccaat tagctgagca acggcattag
    27301 gtatgtcgtt tgaaacaaat tgaatagcat gtgtgttcgc cattttaagt attaatgcgc
    27361 caaatttttc atacacacgc ttgtcgtttg tttgtacata aacagataaa gtaacttttg
    27421 gagaaagtcc atttttattt cgtatatcgc gaatggcaga aataatttct ttaaccattt
    27481 caccatcgtg aataatattc gtattatata ttgcattatt tggatagttg gctacacaaa
    27541 tatcgtcagt tctgtcttct tttagatggt gatatatttc ttcagaaatg aatggcataa
    27601 atggatgcaa tagtttcatc aattcttcga aataggaaat tgttttgtca taagtttttt
    27661 tgtctatagg ctcaccaaaa ggtggtttga caaactctaa gtaccaggaa caaaaatcgt
    27721 cccaaataaa tttatataaa tcaattaaag cttcagataa gcgatatgaa gcatacgatt
    27781 tttctaaatt ttcttttaat aattccaatt tattatcaaa ccattcaaat actatttcat
    27841 tatctgtatg attttcggaa atagtccaac cttttactaa tcgtaaagca ttccatattt
    27901 tattagtaaa atttctacct tgctcacata gcttttcatc aaatggtaaa tcattaccgg
    27961 caggcgagca gaacagcata cctgtgcgca caccatcagc agaatatttt tcgattaatt
    28021 ccaataaatc aggagaattg cccaatgatt tagacatttt tcgaccttgt ttatctctta
    28081 caatacctgt gagatataca ttcttgaaag gtttttcatt tttgtattca tagccagcca
    28141 taatcatacg agccacccaa aagaagagaa tttctggtgc agtcactaag tcattggtag
    28201 gatagtagta tttgatgtca gcaccatcag gatttttaaa accatcaaat acggaaatag
    28261 gccacaacca cgatgagaac caagtgtcga gcacatcatc atcttgtttc aagttattgg
    28321 aattatgatt tggaaattga gctgcatgta tctgtcttgc ttcgtctatt gtttttgcaa
    28381 ccacgcaatt tcctttttca tcgtaccaag caggtatgcg atggccccac caaagctgac
    28441 gagaaataca ccaatcatgt acattttcca tccaatgttt gtatgtgttt ttaaattttt
    28501 ctggaatcag tttgatattg tcatttaaaa cattttctaa ggctggcttt gccaatgtgt
    28561 ccatttttaa aaaccattgc atcgataatt taggttcaat tacggcatct gttctttctg
    28621 aaaaaccaac cttatttttt atttcttcca ccttttgtaa ttgaccattt tcttccaagg
    28681 tagcagcaat ttttttacgt acgacaaacc tgtcttcgcc aacaaatagt tgtgcttttt
    28741 cgctgagtgt gccatcgtca ttaagtgtgt caaaaacttc taaattatgt ttaataccta
    28801 agttataatc gttgatatca tgcgcaggcg ttactttcag tgcgccagta ccaaattcga
    28861 tatcaatata ttcgtcaaaa ataattggaa tactgcgatt gattaaaggc actaaagctt
    28921 gttttccttt taagtgtttg tagcgttcgt catcaggatg cacacagatg gcagtatctc
    28981 ctaatattgt ttctggtcgt gttgtagcta ttgttatcca ttcgtcatcg gtatctacaa
    29041 ttttatagcg aatgtaaaat aatttagaat ttacttcttt gtgaattact tcttcatcag
    29101 aaacagctgt caaccctttc ggatcccagt tgaccatgcg tacgccacga tatatttttc
    29161 ctttattgta taagtcgatg aacacttcaa taacggcgtc acttaaatca gcttccatgg
    29221 taaatcgcgt tctgtcccaa tcgcatgaag caccaatttt ttctaattgt tttaaaatga
    29281 tgccaccata tttttctttc cattgccaag cgtgttctaa aaattgatca cgcgtcaagt
    29341 cggatttatt gatgccttgt tctttcaata aattcaccac ttttgcttca gtagcaatag
    29401 aagcgtggtc agtgcctggc acccaacatg cattttttcc ttgcatacga gctctgcgaa
    29461 tcaatacatc ttgaatcgta tcgtttagca tgtgtcccat gtgcaaaata cctgtaacat
    29521 ttggtggcgg aatgacgatg gtataaggct cgcgctcatc aggaacagag cgaaaatatt
    29581 ttttatctaa ccaatgttta taccacttat tttcaatctg tgtataaact atattttttg
    29641 gtaattcttg catcgctacg aatagttatt tattgtatgt aaagatttat tggcaaaaat
    29701 acgaaatact tactgtacaa cgattagtgt tgtaagctag attgttaagc gacaattatt
    29761 ttttgttaat tactggcaat tggttcaagc gtataacctt ttgccttcag taatgcaatc
    29821 aagccatgtg caccaccaag atgaccagcg ccaacggcaa taaaacaaga ttttttttcc
    29881 attttcattt gcagtgctgg tatccaattg ttgttgcgtt ttaccaataa tttatcattc
    29941 caaatagtat cttcttctgc ctcgaaagcc ttgcttaata cctccagatt ttctgatttg
    30001 taatctttaa tgagtgcatc taatctagca gtctctgacg aatattcttt aatcgttttt
    30061 atcatgcttt ttaattgaac tgcaatagga atttcctcca aaaattgtag ttgttcttcc
    30121 atagtttcta agcctgaaac agtaattttt tttctatcgg caatcttttt taagttgtct
    30181 tcataagact ctttgtcttg cccaagatat tttatataaa tcaattgttc taagtaaaat
    30241 ggttttaatc ttgcatagaa ttgttcaaac gtaaattttt tcacaccaat agaatcttcc
    30301 ataaatgttt gaataatttg gtaatcgcta tcgctcagat aatttttaat tgttttgcca
    30361 ctgtcgagta tgctaatagc agaaagtgca gcaatatcca tgttttccaa gtccatttcc
    30421 atgatgagct catggctgtt ttccagcttt ttttgtacat tttttcccaa aaaataatct
    30481 tgcgtagaga tgatatgaat tgtaccaaat agataagaag gctgagtaag accattgcca
    30541 gatatttgcc aaagcaatga attatttgtg tttagagctt tgtctagttc taatgtttga
    30601 ttattatttt catttttctt tggtggtcta aatccaaaaa gtatggcaac actaagcagt
    30661 aacgctattt ttcttttcat tatttttttg tttttatgta aataaaaagc tgcaataatg
    30721 cagcttataa attgaataaa ttaagtttta ctgaggctgc acccatggtg tcgttcttgg
    30781 gcttttatag cttttcttat actcttctat atccttattt tttgaaggca tccaaaaatc
    30841 ttgacctaaa tagctgatga caggttcgaa ttgcggaaca gcaatgtagc cttttaatgg
    30901 cgtaatgagt ttaaaatctg gtgtaagcac gcaaaatgtt ggataggaaa gctcgttttg
    30961 cataaaatag gcagccaatt cgtgatatcc ttttctaccg cttggcacaa aattccattt
    31021 tctgtctaag aatgtaatag tatcgcttct ttctgcatca aattttacac tataaaagta
    31081 tttgttcatg atttgtgcca ccgtagggtc gtcgaatgtg tttctgtcca tcaccttaca
    31141 ccaaccacac caagaggtgt aaaaatcgat gaaaatattc tttggatttt tttcgtttag
    31201 ctttaccatt tcgtcccaag tcagccattt cactttttca ccctcagtag aaacattagc
    31261 tgtagatgac ttagttgttg ggccaccagg tccactagca aagctagaca tactgataat
    31321 gctaaataag agtattaatt gtattctcat aaaaattgct aagtttattt attgaccaga
    31381 ttgatattgt tgaaaatcca ttgttttata gcttccactt ttaaaataat tcaatacgat
    31441 gcttaatgtt tcaacatctt taaagccagg aaatgcttgt aatttattgc cattttcgtc
    31501 taagactaca atagttggat accctaattt gtttcctaca ctacctaagt ctaatgctaa
    31561 ttgattagca ccacgtgcgc cagtttttac aaaattatat gtcttaccat taaagttaat
    31621 aggagccgtt gtttctgcat taaattttgc agctgtaaaa ttttcattca ttagtgcaat
    31681 aatgccagca tcttgatagg tgttttcttc cattttcttg caccaaccac accaatctgt
    31741 ataaaggttg ataagaataa atttcttttt gctaatttta ttttgaggaa tcgcttgttc
    31801 aatatttgtc catgcaattt gattgtcaac aacaacagca gcttcatttt ttgcatataa
    31861 aatactgata aaacaaaacg ataagaccat taatactaac aacttgaagt ttaatttttt
    31921 catgattatt tttgttatat tcaccaatgt tacaaattta ataccaatta taggaatcgg
    31981 taaatttaaa taattcagcg tggaaagaaa aataattgtt gcagtttcag gcgcaagtgg
    32041 ttcaatttac actaaagtgc tgtttgataa gttgttacat attaaacaac aagtgtccaa
    32101 agtaggtgtg gtgatgtcag ataatgcaaa agatgtgtgg aaatttgaat tgggaaatga
    32161 agattataca tcttatcctt ttgattttta tgccaaaaac gattttatgg cgccatttgc
    32221 ttccggttct gcaaaatatg acacaatgat tgtttgtcca tgttccatgg gcactttagg
    32281 cagaattgct ggtggtatct ccaatgattt aactactcgt gcagctgatg ttatgctgaa
    32341 agaaagaaga aaattaatac tcgttacgcg agaaacgccc ttgcacctta ttcatatcaa
    32401 taacatgaaa attgtgacag aaagtggtgg tattatctgc cctgcaacac ctagttttta
    32461 tagccttccc aattcttttg aacaattagc agcgacggtg attgatcgtg ttattgattt
    32521 gtgtggcttt gaaaataata gctatcgctg gcgagaatga tttctttaaa tttatttttt
    32581 tctgatttcg aaataatcgg taaagcttgt ttttcgataa ttttctttga taaattttat
    32641 gataggatag cttaatcctt tattttcttg tggtgaaaaa ccatctttta aaagtgcggt
    32701 ttgaaaaata ggaatggcaa ataaaagagc agtgtcatcc tttattttag aagtaacttt
    32761 tcccaatatt ttctcgttcg gaatttccgt tttattaggc cattctgtca atatttttga
    32821 ttgatgtgaa tgcaatatat tacatgctgt aaagtctgga acagttacta gattttgtgt
    32881 cgttttttta agtgagtcta attccaaaag tacgctataa gtatttttat tagtgtaaat
    32941 tccatgtgca ccttcaacga tattatctaa tttataacgt agatattttt ttggcatatc
    33001 tctataaata ttgttcgtgc gaacaaagta aaatattgaa attcctacca atacaaacag
    33061 caacagtacc ttgttttgga ttctactttt aaaagaattg aattgcatga aaataaggat
    33121 tgaaatcata ccaccagcaa ataatgcagg cgaattataa ccaactgaaa tggacacaga
    33181 ccaagctatt attaaagcaa taaatggtag aatacaattt tcttgtttta ctattttatg
    33241 tgtggtttca cctgcaataa gtccgagaaa caaaaatata aatttgccgt gataatgatt
    33301 gctgattaat aaaatggatg caaatgaaaa tattaagatt gatagatata ttttatttat
    33361 tttcattttg taataaacga aaacactaat aaaacctata tacatcaatg gatttgataa
    33421 atatgaatag atgccaacgt ttactatttc attgtgacca cttaattgaa tagaaagatc
    33481 atgccatcca ttattcaaaa gaatatatcc aacataaatt attatgggaa gtatgcctat
    33541 caacatattt ctgattatat tttctcttcc aaacaaataa atagtcatag gtaaaataat
    33601 caagtaattc tgtttgcaga ttgcagcata tcctatgaaa aaatagccaa tataattcca
    33661 ttttttttca ctaattataa agtaaattcc gagcacactc agcaacaaac catcaattgt
    33721 atgtaatacg gaagctggaa aataatgcac attaaatagg aagcaaataa ttatcaatgt
    33781 gtatttcaaa aatagtgtca cttctgtatt tgaatacctt ataatgatac taatccaaaa
    33841 ataagcaatc agtacttgct ccaaccaaaa gataaaacgt gacattaaaa acacatgcgt
    33901 ttttgaaaat aacaattcag gaatatgtaa gtacgcatat cctaatggcc tgacagaaga
    33961 aaaatcgacg tgtggaattt gtccatgcat caatctattg cttgctgata acacaaagcc
    34021 ttcgtcagtt ggattaaacc caaattttga aaacaataaa tgacaaatta tcgaaatgaa
    34081 taaaataaaa atgatatcat acgctgtgtt cttcgccatt taaacaaaga taacaacttc
    34141 taacacaatc ataaactatt ctatttttag aagtataatg ctttgcgtac ttttaccgta
    34201 attaatatat tatggaatat agtttctcag aaattgaaaa gattgctaga caaaaatggc
    34261 aagataataa tgtttataaa gtaacaacgc aaagcgataa acctaaatat tatgtattag
    34321 atatgttccc atatccaagt ggtgctgggt tgcatgttgg acatccgctt ggttatattt
    34381 tttctgatat ttatgctcgc tacaaacgta tgaaaggctt taatgtatta catccaatgg
    34441 gttttgatgc atttggttta cctgcagagc aatatgccat tcaaacaggt cagcatcctg
    34501 aaattactac tcaaaagaat attgagacct atatcagcca attacaaaaa attggatttt
    34561 cttatgattg gtcacgtcaa gtaagcactt gcgagcctgc atattacaaa tggacacaat
    34621 ggattttctt gcaattattt aacgcatatt attgcacaag taaacaacaa gcattttcaa
    34681 tagcaaattt agttcggcaa tttgaagaga atggaaatat gcaaaataca gcatatacaa
    34741 gtgctgatgt ttcgccattt actgcgcttg agtggaacgc gttttcgcca aaacaacaac
    34801 aagatatttt aatggaatat cgcttggcat atcagaaaga atctttcgtg aattggtgtc
    34861 cagcattagg cacagtattg gcaaatgatg aaattgtaaa tggtgtgtct gaacgcggtg
    34921 ctcatcctgt cgaaaagaaa aaaatgtcgc aatggttttt acgcatcaca gcatatgccg
    34981 aacgtttgct acaaggccta gaaacattag attggagcga aagtatgaaa gatatgcaac
    35041 gcaattggat tggcaaatct gtaggtgcga tggtgtcatt tcccttgaaa aatttcgaag
    35101 ataaaatcga agtctttaca acaagagtgg atacaatttt tggtgtttca tttttagtaa
    35161 ttgcaccaga acatgaactg gttaagcaaa tcacaacaga tgagtataaa gcagcagttg
    35221 acgagtatgt aaaagtgtgt gcagcaaaat ctgatatcga acgtcaagca gaaaaggtag
    35281 tgtccggaca atttacaggt gcttatgttt tacatccatt tttgcagaca gaaattccag
    35341 tctttatagc agattatgtg ttagctggat atggtacagg tgcagtaatg gcagtacctt
    35401 ctggcgatca acgtgattat ttatttgcta aaaaatttga actaccaatt ataccaattc
    35461 tagatgcgca acagaatatt gagcaagaag ctgatgcaac aaaagaggga aaatatatca
    35521 attctgattt tataaacggc atgcagtaca aagctgcaac ggcattgctt attgaaaaat
    35581 tagaacaaca aaaattagga tatggtaaaa taaattataa aatgcgcgat gctggatatt
    35641 cgcgtcagcg ttattggggc gaaccttttc caatttatta tgataatgat ggcattattc
    35701 atacgatgga agataaatta ccagtttgtt tgccaaatgt tgcttcttat aaaccaaccg
    35761 gaacgggcga aagtccatta gcaaatgcca aagattgggt tactcaaaat ggtgttggaa
    35821 cacgtatgga aacagataca atgcctggtt atgcgggaag ttcatggtat ttcttgcgct
    35881 acatggatcc tgaaaatcct aacgaattag tgagcaaaga ggcacaagaa tattggcaaa
    35941 atgtcgattt ttatgtcggt ggctcagagc acgcagttgg gcatttatta tacagcagat
    36001 tctggcataa atttttaaaa gatattggta aagtaagcac agaagagcca ttccaaaaaa
    36061 tggtcaatca aggcatgatt ggtggtagaa gtttactaac taaaaaagga cagattaaaa
    36121 atgtcgaaat tgcattacat attccagtaa tgctagcgca gcaggataaa ctatataaga
    36181 ataaatttca ggaattaaag gataatgaca atagatttga aaatattcaa attgatgata
    36241 ttgcatggga aacagacaaa gacggcaacg atttcattgc tttagatgtg caattagaaa
    36301 aaatgtcaaa acgctactta aacgtggtaa atcctgatga tatggtggca caatatggcg
    36361 ctgatacttt tcgtatgtat gaaatgtttt taggaccaat agatacagca aaaccttggg
    36421 acaccaatgg catttctggt gtttccaatt tcattcgcaa attctggcgc cttttctacg
    36481 atgaactagg taatgcaaaa aatattgacc ataaacccac gcaagaggaa ttaaaaattc
    36541 tacataaaac aattaaaaaa gtaaacgaag atatcgaacg attagccttt aatacttgcg
    36601 tctctacttt tatgattgct gtaaatgatt tatctaaatt aaaaaatgta tcatctgtaa
    36661 ctttattaga tttaataaaa ttaatagctc cttttgcacc gcataccgca gaagtgttat
    36721 ggacaaatct tggtaataac tcttcagttg taacatgcga ttttccaacg tctgatgaac
    36781 aatatttagt ggaatcaaca ttcaattatc cgattcaaat taatggcaaa cacagagcca
    36841 atatagaaat atcattagaa acgccacaag aagaaattca aactattgta ctgtctgatg
    36901 aacgcgttac caaatttaca gaaggtaaat cacctaagaa atttatagtt gtacaaggtc
    36961 gaataattaa tgttgttgtg taggtatgtg taatgtgttg tttgtgaatg tattatgtgt
    37021 tattttttct acttttttga tttgttttta ataaatcatt tatttatata attacttcat
    37081 gttcaattgt ttatagtttt attgtagatg ttaaatttac acttaagtgt caataaactt
    37141 tgcttagttt tatttaattg ctaaatttac agtattaaag ccaacaagat gattaaaaaa
    37201 attacactaa catgtatgtt agttgtagcc atgtttatcg cctttgctaa agaacataaa
    37261 caggctgcaa ttgatttctt acaaaaaaac ttgacagaaa ctaagcttac tagtcaagac
    37321 attaaagact tgatattact cgatgattct tatgacgatt tcagtcatat taatcgcgtg
    37381 tggttacaac aaacagccta tggcattcca ttaaaaaatg gtatgctctc cgttcatttt
    37441 atgaatggta aagtggtcaa ttatacaaac aatggtattc aggatttgaa aaataaaatt
    37501 tcatctgtta cgccatcaat cacagcagaa aatgccgttc gttttgcagc agctcatgtc
    37561 ggtgtaccta gtatcggtaa aataaaatta caagaaaaag tcaacaaaaa agagaactta
    37621 tacatcttta aacctattga tggtttgact aatcttgagg tgcatgctca attactattt
    37681 acacgtgata aaaatgataa agtggtatta gactgggaag ttcaattcca ttctttagac
    37741 caatataatt tttggtgtat tgatgtaagt gcaaaagatg gcagccttat taaaaaaaca
    37801 gaccaagtat tacattgcga ttttggtaaa ggagaattct taggcagaca tacgcacgag
    37861 acagaacata cttctattaa ttatagaatt ttagatgaaa atttaccaag tggtgcaaca
    37921 gatggcgtat cacaaacttc tgctggttca tatcgtgtat atccatttac tgtagaatct
    37981 ccaatttatg gcgctcgtca gctccttgtt gatccgcatg atgtagttgc ttcaccttat
    38041 ggttggcatg atacaaatgg tgctgcaggt gctgaatata cttacacaag agggaataat
    38101 gtatgggcat atacagataa atatgatgca gatcccaata caccaaccag cttatctact
    38161 gatggtggcg catctttagt ttttgatttc aacttagatt ttactactca attagataca
    38221 ttaactaatt cgaaagcaat tactacacag ttattctatg ctaacaatat catgcacgat
    38281 attttcttta aatatggatt taatgaaagt agcagaaatt ttcagtttaa aaattattct
    38341 ggtgcaagcg gtggttcaga cttagtgtat gctgaagctc atgatgcctc attagcaaat
    38401 cctcgtttga ctaataatgc tacctttgca acagcaccag atggcactag cacgccattc
    38461 aatcgtacta gaatgcaaat gtatatgtgg gacggagctc caccttcttc tttaacttat
    38521 aatgctcctg caagcattgc tggtgatatt gatcatggct cagcaaacgg ttggggacca
    38581 tgttcctata atgttactgc accagttgct aatgcaacca gtaatacagc accaccgagc
    38641 tatgtttgtg gagctgtcaa caatccaggt gatattgctg gtaaaattgc actaatagat
    38701 agaggtactt gtaattttac agagaaagtg tataacgtgc aattagcagg tgcagtaggt
    38761 gctatcatca tcaatagaca atcagcaggt gattcattaa tcggaatggc atcaggtaca
    38821 aatggtggtt tagtaactat tccagctatg tttgttacgt atgcagatgg tcaaaaatta
    38881 cgtgataatt tagccacagc aaatgttact atgtatagaa attcaacaac taactgctta
    38941 gaccttgatg gttctttaga tttcggtatc gttgctcatg aatatgcaca tggtatttct
    39001 acacgattaa caggtttgag tttaggcaca ggtggtacta gtggctgttt aagtaatgaa
    39061 gaacaagcag gtgaaggctg gggcgacttc ttcgcaatgg cattaactaa aaaacctaca
    39121 gataataaaa acacaccaag aggtatgggt aattatgctg ctagccaaga cgtaaatggt
    39181 caaggtatca gacgttatcc atattcttat gatatgtcaa taaatccgtt aacgtatggc
    39241 gatttaaact tgaatcctga aaaacatgat attggtcaag tttggacatc tgcattatgg
    39301 gatatgtact ggttacttgt tgaaaaacat ggttacagca atgatttata taatggtact
    39361 ggtggtaata accgtgcaat taaaattgta gttgaaggtt taaaacaaca aaaatgtaat
    39421 cctggatttt tagattctcg aaatgcaatc ttaaaagcgg actctatatt atatggttat
    39481 gctgataaat gcgaaatatg gacagcattc gcaagacgtg gtatgggtgc ttctgctaat
    39541 caaggaagcg ctggaagcgc ttctgaccaa actgaagcat tcgatttacc tgctgcttgt
    39601 aacaccacac caacagcgac agcgagtttt acggtcagcg acaccacggt atgcgtagga
    39661 ggtagtttaa catttacgaa tacctcaacg gcaagcagtg gcagtccgg
//
LOCUS       DAOQQV010000010        39517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_281_length_39517_cov_7.612285, whole genome shotgun sequence.
ACCESSION   DAOQQV010000010 DAOQQV010000000
VERSION     DAOQQV010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 39517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39517
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_281_length_39517_cov_7.612285"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..179
                     /locus_tag="PLL59_02210"
                     /pseudo
     CDS             1..179
                     /locus_tag="PLL59_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582813.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
     gene            complement(411..1619)
                     /locus_tag="PLL59_02215"
     CDS             complement(411..1619)
                     /locus_tag="PLL59_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMY22645.1"
                     /translation="MKDYSSESLALHEKLKGKISISLKTSLETKDDLTLCYSPGVAEP
                     VRQIANDVEKVWDLTIKANTVAVVSDGSAILGLGNLGPEAAIPVMEGKAMLFKKFADV
                     DAFPICLNTQNTEEIIQTIRNIAPVFGGINLEDISSPRCFEIEERLQTLGIPVFHDDQ
                     HGTAIVVLAGIINACKLLNKKIEDLNVVINGAGAAGLAISKLLMCFEIDKSVCTPVKQ
                     IIMCDTKGIIHKDRADLNDFKREILRHTNPNNIKGTLKTALKNADVFIGVSQANQLTK
                     EDVMSMNPSPIVFPLANPIPEISPDLAYEGGAGVVGTGRSDMPNQINNVLAFPGIFKG
                     ALEIRARRITPRMKVAAAYAIADCVTELSRDKIIPSTLDMSVAEKVAQAVKDAYEEDM
                     KRMETPRHAL"
     gene            complement(1793..2704)
                     /locus_tag="PLL59_02220"
     CDS             complement(1793..2704)
                     /locus_tag="PLL59_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22646.1"
                     /translation="MFYIKTINMKCHRLFLLFFFALIYTSIQATELTLFKALTVEPNV
                     TKDGVKGMTIIYTFNLTPIEDINPKDTVLKNASFVFKTTFSENDKPIKSAKGYNSVSN
                     ETGNVVIERYLSLTETEATKNNQTIRQFVPYAALQLDSGKHTIDVTAVFSGKDGNDIA
                     YKTTLKKPQVQILKPKVKIFTMDIDYIEVNDFNRSGQAWDYAFFKTDAPDVGVNILIG
                     GTSVYKAHVNDTHMFAVGPNSKNIIFAISENDKVKVLIQDIDVMFNDDIANWDFYTSD
                     KKTNTVYTYNKSKGNIKSCNLTFKITD"
     gene            complement(2780..4036)
                     /gene="ilvA"
                     /locus_tag="PLL59_02225"
     CDS             complement(2780..4036)
                     /gene="ilvA"
                     /locus_tag="PLL59_02225"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HMY22647.1"
                     /translation="MTSELPKTKGIIQAKKNLEGIIEVTPLKLNQILSDETGAKVYLK
                     REDLQVVRSYKIRGAYNFISQLTKEQKKLGVVCASAGNHAQGFAYSCQLLKIHGVVFM
                     PSPTPRQKIDAVRRIGKNFIEVILSGDTYDDAKSAAQKYISTHKKILVPPFDHTLIIE
                     GQGTVAMEILEQIGKKKIDYLFVPIGGGGLAAGLITYFKEFSPNTKIVGVEPLGAPAM
                     KVSLEKNKVVTLSQIDNFVDGAAVKQVGQIPFQLCKKNLEEVVLVPEGRVCTKIMQLY
                     NSDAIVLEPAGALSIAALDYYKEKIKGKNVVCVISGGNNDIGRMPDIKERSLIFEGLK
                     HFFIIKFPQRAGALRQFVTKVLSANEDITLFEYTKKNSKESGPALVGIELKYKDDYDL
                     LIKRMKKYKVDFVPLNDNPMLFNFLI"
     gene            4172..4684
                     /locus_tag="PLL59_02230"
     CDS             4172..4684
                     /locus_tag="PLL59_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HMY22648.1"
                     /translation="MNKAGIISILTYLIFISNISFAVESDTIAATIKIDSIYVDKSDR
                     VLIVFSKQQKIKSYSIGLGFQPIGKKQFEGDGKTPEGLYFIDTKSAQSKFHKNLNISY
                     PNNNDKAYAKLQQKNAGGDIKIHGLPNGVEDKHYEVNDWTLGCIALRNKEIDELFKHI
                     RIVCPIYIAP"
     gene            complement(4698..5192)
                     /locus_tag="PLL59_02235"
     CDS             complement(4698..5192)
                     /locus_tag="PLL59_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMY22649.1"
                     /translation="MPLQDRVILDMQIDFEEFFTHKIPAIIDSIKGDEPAKWGLMTVH
                     HMLEHLVLPLNFAIGTLPMSTMIAEEKIPRQLAFLASEYGLPKNFKPAFLPSDKTLPL
                     MYADIVQSKQLLKDTIVKFLASVNATTFANATHPFYGPLDKTGWLQFQYKHFIHHFSQ
                     FGLL"
     gene            complement(5196..5342)
                     /locus_tag="PLL59_02240"
     CDS             complement(5196..5342)
                     /locus_tag="PLL59_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22650.1"
                     /translation="MNAPKSIDTKDSVYYKMPIYKQGFFCTFEDMLNRRKVPVDFSLG
                     NSKY"
     gene            complement(5505..5897)
                     /locus_tag="PLL59_02245"
     CDS             complement(5505..5897)
                     /locus_tag="PLL59_02245"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HMY22651.1"
                     /translation="MPEYLFTYGTLRLNQSHPIATYLAENASLYGLAILPKAKLYKID
                     WYPALIETTNEADEVVGDVFVLNNDTAWSKIDEYEGIGIGEPPYEYRRTKVKIKTDAG
                     ELECWVYFYNLPLPADASCIESGDFLNP"
     gene            complement(5960..6658)
                     /locus_tag="PLL59_02250"
     CDS             complement(5960..6658)
                     /locus_tag="PLL59_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22652.1"
                     /translation="MKTILTIIMLMLLFDVSANNDTSASVDIYQQKRKPLFFKTGGRI
                     RLQFGGQIGANLNYFKYKNGTQRDEAAGYQGGLFLRVSRQMAFVQIEVNMLRSMVFLK
                     NGVFNREIGNNIPFEQLRLKYYTVGIPFIFGAYAVKNPLYKLRFYNGIEAAFITKIKA
                     TIEQNNNEIYRLSKKEKRRIFRPEQFSYQLGMGMDIAMFIFDIKYSVGMRSFFKENYR
                     TQTHLFQFTIGAIF"
     gene            complement(6660..7559)
                     /locus_tag="PLL59_02255"
     CDS             complement(6660..7559)
                     /locus_tag="PLL59_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HMY22653.1"
                     /translation="MQQEDGMVLIAGGDFEMGGNNQQARQDEFPKHKVRISAFFIDKT
                     EVTNAAFKQFVDATNYVTTAEKDFNFKDENGVVVHQKAGSLVFNPIKKGDVANPNTWW
                     KFVEGANWKHPQGPHSTIEGKDNFPVVQVSWYDAEAYCKWANKRLPTEAEWEMAARCG
                     LQNALYTFGNDRHGLFKKMNSWNGDFPYSNTALDGFERCAPVMSFPPNKNQLYDMAGN
                     VWEWCSDWYDEKYYNKCVEFSNFKNPENKIANAALEKSMRGGSFLCNDSYCSGFRVSA
                     RMKSSAETSLEHTGFRCVKDVEQ"
     gene            complement(7697..9337)
                     /locus_tag="PLL59_02260"
     CDS             complement(7697..9337)
                     /locus_tag="PLL59_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMY22654.1"
                     /translation="MSIKSNKKLLIPVILSLFVGIFFVACYPLSTGAIHWRIKFDKEK
                     IAQKKAYLSDTILVNEKQPNIVLIVADDLGLNDVSCYGNSTVQTPNIDALANEGVKCV
                     EGYVTSAVCAPSRCGILTGRYQQRCGFETQETEYYPTNIIEYLTGRYISEKDTNWVVA
                     TKPRYPREWQIAKQGVPPTEITLAELLKKANYSTAIIGKWHLGKNPKFNIPSVRGFDY
                     QYGCYGAFTMYVDNQIQEDIVNYRRQSFSAKYQWDMARRDDAMIYQNNKKIKHEKQYL
                     TDAIRDRSIQFIENNKQNPFFLYIPFTAPHEPYQAPLDLYVKEFEHVKDKKKAVYNAI
                     IRSMDNAIGAIHQKIKDAGLDDNTLIIFLSDNGSASYSGITSNEPLKGGKLTPYEGGL
                     NIPFIFKWKNHLPENVVYRKPIISLDIFSTVAAVSNIALPKDRVYDGVNLMPFFKQEI
                     DTAPHDKLYWRTDHVHAIRKGDYKLIYSTRDKWKELYNLKEDKGERNNLFFSMPEKAA
                     DLENDLMEWEKTLPVKPMWPRIMDYRFMIDGKSYLFPA"
     gene            9370..10002
                     /locus_tag="PLL59_02265"
     CDS             9370..10002
                     /locus_tag="PLL59_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HMY22655.1"
                     /translation="MKIEIKKEIANAITHGIGFVTFATLLPLLFIKASHYDVNYKLVG
                     LIFFAIGLVAVYLSSTIYHSLTNERAKYILRIADHISIYYLIAGTYTALLMQTIPFNK
                     IMWFIIILWSVVLIGTIKKIFLTGKYDTISTIIYVVMGCMGLFQINTIMKFTPDKVLL
                     FILLGGIAYLLGVIFYAWKKFTYHHAVWHLFVLAGSIMHFFGVWYAIEKP"
     gene            10080..11453
                     /locus_tag="PLL59_02270"
     CDS             10080..11453
                     /locus_tag="PLL59_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HMY22656.1"
                     /translation="MHKTANKGIFYYIIGDYLAALLSWLCFFIFRKLQIENVTHSISR
                     ILQDEKIIHGLVLIPICWLIYYFYDNTYTSLLKKSRLHEIQQTAKQTLIGGILLFFSL
                     MLNDFIKKYNDYYWLFVGFLAFHFFTTILLRLLILGIVKNRISNGRLTIPTLLIGNFE
                     NCQKVEQEIEHSKVKLPYKIQEKIIIDNANLDSITPKNKDFENVILAFGTTSAQHFEK
                     IIFHFLNQGISVQLLPDELDILSGKFKTTSVFGSPLLEIPTTLLKPWEKTSKRAADIT
                     LSFLGLFFLLPFLVFIAIRVKLSSKGNIFFMQERVGLNGNIFRIIKFRSMVEDAENGT
                     PRLSSEHDERITPFGRTMRKYRIDELPQLWNVLIGDMSLVGPRPERQFFIDQIIQTAP
                     QYQLLQRVKPGITSLGMVKFGYASNLTEMLQRMRYDLLYIENISLQMDIKIMIYTVII
                     LWKGKGK"
     gene            complement(11473..11904)
                     /locus_tag="PLL59_02275"
     CDS             complement(11473..11904)
                     /locus_tag="PLL59_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMY22657.1"
                     /translation="MLSLTCKAAIKAVVCLGSNKESHKKLSIREIAQQIDENEHTVGK
                     LLQKLVKDKIINSTKGPNGGFFISENQVHLPVIKVIESIDGMRVFKECGLGLDHCSDD
                     RPCPMHEDFKPIRDMFTQMCSKKKIIDMYEPVNNGLVYLLG"
     gene            complement(11911..12234)
                     /locus_tag="PLL59_02280"
     CDS             complement(11911..12234)
                     /locus_tag="PLL59_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HMY22658.1"
                     /translation="MHIETNNENLSAIALEGLKAVIDPEIGLNVVDLGLIYSLLFDET
                     AKRINCVMTLTSQFCPMGESIIEDVNNSLQSSFDSYGIDVNLTYEPTWNADMISEEGQ
                     LFLGR"
     gene            complement(12236..13522)
                     /locus_tag="PLL59_02285"
     CDS             complement(12236..13522)
                     /locus_tag="PLL59_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HMY22659.1"
                     /translation="MFVAQGNTTLAKTTSYKIVLPFYLYAAVSFLLATVLLVFSSAQF
                     LIHYFHPTILAITHTMALGWATMIIIGACHQLVPVLIEGELYGNKLAYATFVLMAIGI
                     PLLVHGFYVFNVGVFTQGGGLLIIFALLLFLYNIGKSITQSKHENIHAIFVFTAIIWL
                     LLTAILGWILLFNFTTMVLPYDSLHYLSLHAHIGIAGWFLLLIIGVASRLIPMFLISK
                     YNNTNLLWWIYACINLGLLLFGVLFFLTAKLIYYAFPIFMVSSGIFIFMFYCLRAYQQ
                     RIRKQVDAQMKLSVFSVMIMAMPIVFLILLLLLMTYSNVNQIRLATAYGFVLFFVWIT
                     SIILGMTFKTLPFIVWNKVYHHRARIGKTPNPKDLFDDNIFKLMSFCYLIASVLLLSG
                     ILLSNVILISCGAVGLLCTAILYNYNVFILLKHKPV"
     gene            complement(13524..14330)
                     /locus_tag="PLL59_02290"
     CDS             complement(13524..14330)
                     /locus_tag="PLL59_02290"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HMY22660.1"
                     /translation="MKINKSTKISAIIAHNKSAIDEIAAINPHFKKLQNPILRKILAS
                     RVTIEDAAKIGKCTVEDFYTVLSKIGFEIEEINQASINTQTSDKQDIKQALANKKLVA
                     LDVRQELENGIDPFNLIMQHIGKLQSDEVLQIINTFEPTPLIAILRKKGFIAVVETIN
                     ENLIHTYFYKTDTTSKVETVDIQQASDFDDVLQKYQQKLTTIDVRDLEMPLPMHTILS
                     ALDHLASDSALFVYHKRIPVFLLPELADRKFEYRVKEISANEVHLLIFKA"
     gene            complement(14374..14850)
                     /locus_tag="PLL59_02295"
     CDS             complement(14374..14850)
                     /locus_tag="PLL59_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22661.1"
                     /translation="MGISYIISVYLHILFAAIWIGGMLFLPLVLLPSIKQVSNKIDIL
                     YATGIKFRVYGWMSIFGLILTGAYNMYAKGIPFSITYLATDTYGKLFGTKLLLFSCML
                     TIVTVHDFYIGNKAIDQMRIDNSTKFRIIARWSGRINLMLSLAIAFLGVAISRGGF"
     gene            complement(14851..15810)
                     /gene="ric"
                     /locus_tag="PLL59_02300"
     CDS             complement(14851..15810)
                     /gene="ric"
                     /locus_tag="PLL59_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HMY22662.1"
                     /translation="MNTTAENVLNVTLLEPRMKHPTIFARFDELLEGESLTIHNDHDP
                     KPLYYQLLGERGNVFSWEYLENGPIWWKVRIGKNKTGEAAETLGEIVTKDLRKAQIFK
                     KFGLDFCCGGKKTVKEACAEKGIDVTLVETELLQASRTVAGRALPYNEWSLDFLADYI
                     VQTHHAFVKNTLPDMKMYAAKVAKVHGAQHSELARIEALVEEISAEFESHMDKEENVL
                     FPYIKAMVMAKNTLSPLKAASFGSVKSPITMMEMEHENAGKIMEEIRTLSNDYQLPQD
                     ACASYGLLYKLLSDFEEDLHLHVHLENNILFPKAQELEKTLLA"
     gene            complement(15953..16363)
                     /gene="rnk"
                     /locus_tag="PLL59_02305"
     CDS             complement(15953..16363)
                     /gene="rnk"
                     /locus_tag="PLL59_02305"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HMY22663.1"
                     /translation="MSRLIVNRLDYARIKKSISDARDFKSINKSESDKLMKELDSAEI
                     VEPEAIPSNVVTMNSIVKLSFLNNDRQIQFQIVYPEQANMKENKISIFSPIATALIGY
                     KVKDEIEWIVPAGLTKIRIDEIIYQPEAAGDYNL"
     gene            complement(16533..17507)
                     /locus_tag="PLL59_02310"
     CDS             complement(16533..17507)
                     /locus_tag="PLL59_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMY22664.1"
                     /translation="MSELTIKLETVDPVEFFGFNNNKLKILKKEFNRLNISARGNKII
                     VAGSQEDLEVFSDKVHQLVEYLERYGKISDQHLEEILHGRNPYETNFPQFQDNVLVYG
                     NNGTLVRARTENQQKMVDLSEDNDILFAMGPAGTGKTYTAVALAVRALKNKSVKKIIL
                     TRPAVEAGENLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLAYYMQNRIIEVAPLAY
                     MRGRTLDNAFMILDEAQNATTLQLKMFLTRIGPSAQCIITGDMTQIDLPKNQQSGLRK
                     TMQILDGIEGIGMIHLSADDVVRHRLVKDIIKAFDKDAEREQQFKNNN"
     gene            17603..18370
                     /locus_tag="PLL59_02315"
     CDS             17603..18370
                     /locus_tag="PLL59_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HMY22665.1"
                     /translation="MAVITLLTDFGERDYYVGLFKGDLYTSSPSAKIVDISHSIPPYD
                     IVTAAFFLKNVYAHYPKGTIHIARVFEQGLGTQKILAAKYQNYFFIAPDNGLLTLVFD
                     EKPDLIVEIDMKQAKLRTLEEYYCRVVKEITFNNNIGSIGVATSNYVEKRALLPVIEE
                     NRLIGNVIYVDNFGNVITNIHIDDIKRFDTSKQMRINYRKVDFIDRVVENYSDVPRSY
                     SLARFNSIGYLEICINGGNASDLLGLKNGDAIQILFE"
     gene            18367..18663
                     /locus_tag="PLL59_02320"
     CDS             18367..18663
                     /locus_tag="PLL59_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HMY22666.1"
                     /translation="MIIRIVKLTMREEEVATFKNYFATVCETIRAQEGCTMLQAWQDI
                     HQPNIFFTYSIWHTETDLNNYRDSAFFLQFWKTVKPWFAAKAEVWSFDKIVDLP"
     gene            18689..19738
                     /gene="aroB"
                     /locus_tag="PLL59_02325"
     CDS             18689..19738
                     /gene="aroB"
                     /locus_tag="PLL59_02325"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HMY22667.1"
                     /translation="MNTISLNDYSIYLDDSLQSLYRFIEEKNYSALFAIVDENTKQHC
                     LPIISEHIVGLHIIETQSGETNKNLYTCEKIWQQLITKNADRKILIINLGGGVIGDMG
                     GFAASCYKRGVDFINIPTTLLSQVDSSIGGKLGIDFKYGKNLIGLFQNPKAVFISSVF
                     FNTLPQRQFINGWAEIFKHALIQDSAQWNIYKQYDILSCHMDEIVFHSLLVKKKVVEI
                     DPYEKGLRKVLNFGHTIGHAIEAYSLEHESTPLLHGEAIAIGMICEAYLSVKKCGLTA
                     DELNEIQTVLLKHFPKHDISTFDMNKIIESMRIDKKNEGDTIMAALLSAIGNSVYDIP
                     LTTEDIQESIAFYIQ"
     gene            19764..20255
                     /gene="tpx"
                     /locus_tag="PLL59_02330"
     CDS             19764..20255
                     /gene="tpx"
                     /locus_tag="PLL59_02330"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HMY22668.1"
                     /translation="MATITFKGNEVHTAGNLPAIGDSLSNFSLVASNLSEKTLADFSG
                     VQLILNIFPSIDTGICAASVRKFNQEAAALANTKVLCISKDLPFALSRFCGAEGIENV
                     ETLSAFRSHDFDYVLMADGPLKGLLSRCVIVVDADGKVVYAEQVAEIATEPDYTAALA
                     SLG"
     gene            complement(20264..20947)
                     /locus_tag="PLL59_02335"
     CDS             complement(20264..20947)
                     /locus_tag="PLL59_02335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY22669.1"
                     /translation="MNIVITGASRGIGKSIAAKFFNNGHSVIVCSRDANKLRQLQIEC
                     PSILTFVCDVSVKKEIDNFADFVLSNFKTIDVLINNAGVFIPGKITEEEDDVLEKTLQ
                     SNVYSAYYLTKKLAPTFIRQASGYIINMCSIASIMAYENGGSYSISKFALLGFSKTLR
                     EELKPYNIKVTAILPGATLTDSWQGTDLPESRFSKPEDIAELVYTITQLSEFSVVEEL
                     IIRPQLGDI"
     gene            21042..21806
                     /locus_tag="PLL59_02340"
     CDS             21042..21806
                     /locus_tag="PLL59_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMY22670.1"
                     /translation="MAEYIHASGGPLFHIGRYILMVKRCFVKPERAAMYWKETLRQMA
                     NIGVGSVMIIILISFFIGAVSTVQFAYNLSQSMVPMYYVGYVVKESMILELAPTISCL
                     ILAGKVGSNLSSELGTMRLTEQIDALEIMGVNTISYLIGPKIAAAITMIPLLVVVSAL
                     TGIWGGMLAGDLTGYVSGVDYTQGILMNFVAKNIYIMLTKAAVFGFILTSISCYQGFY
                     AYGSSIEIGKASTRAVVLSSIMVLIADFVLAWMLLN"
     gene            21811..22593
                     /locus_tag="PLL59_02345"
     CDS             21811..22593
                     /locus_tag="PLL59_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMY22671.1"
                     /translation="MIEVKDVHKSFGSQHVLKGVSSLFEAGKNNLIIGKSGSGKTVLL
                     KCIVGLLNPTEGHVLFDGKDYVAMNIEQKNEIRRELGMLFQGGALFDSLTVEENVRFP
                     LDMFTKMAMSDKKDRVNEVLKRVNLVNANAKYPSQLSGGMQKRTGIARAIVMNPKYLF
                     CDEPNSGLDPQTSIVIDELIMEITEEYKITTITNTHDMNSVITNGDKIIFLHEGNKHW
                     EGDKISLFESDNKELIDFIFASEFLKEARDARHKKLLSNLSK"
     gene            22596..23468
                     /gene="sucD"
                     /locus_tag="PLL59_02350"
     CDS             22596..23468
                     /gene="sucD"
                     /locus_tag="PLL59_02350"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HMY22672.1"
                     /translation="MSILVNKNSKVIVQGFTGKEGTFHATQMIEYGTNVVGGVTPGKE
                     GSTHLDKPVFNTVENAVKATGANVSIIFVPPAFAADAIMEAADAGIQVIVTITEGIPV
                     QDMIKAKKYVTSKGVRMIGPNCPGIITADEAKIGIMPGFVFKKGKVGIVSKSGTLTYE
                     AADQVVKAGFGVSTAIGIGGDPIIGTSTKEAVELFMNDEETEAIVMIGEIGGTMEVDA
                     AMWLKDNMKKPVIGFIAGQTAPKGRRMGHAGAIVGGANDTAAAKMKIMGECGIHVVDS
                     PAFIGKKVAEVLAK"
     gene            23596..25698
                     /locus_tag="PLL59_02355"
     CDS             23596..25698
                     /locus_tag="PLL59_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22673.1"
                     /translation="MNTTFIAAYPWWFILFCIALGVLFSYILYGKKKFIFTENESKWW
                     KYGLATLRFLSTSLIAFLLLSLLIKSKHTEEEKPLILLLQDNSSSLHISFGNFSKEKY
                     ATQLSALQEKIREKYDIISYNFGNTLSNFKTPNYTEKETDISNAIDELYMRHGTQNIG
                     AIILASDGIFTKGNSPIYNKNALTIPFYTIALGDTSIKKDALIKSIRYPEIVYLGDQF
                     TINIQIEANHLQGQTTVLEVIAPDGHIILSKPIEITEDRFSIQSDAIAEATKPGILRY
                     QIRLKTLTGETIVENNTDIAYIEVLDGRQKILLLYDAPHPDVKAFKNAIEQNKNYQFE
                     SADIKNYNANIADANLIILHGLPSTDAISKINKIQEIANSNLPILWILSANTNVAQLN
                     SIQKIVQISGASLNGNDVFPIYQNSFSKFTINAPSLQTINALPPLLAPYGRYTLASTA
                     DVLFRQQIGTIPTDNPLIVFNDINGKKTGFICGEGIWRWRMNEYAQTNTFMATDELIN
                     KCVQFLTVKGDKRRFRVHTPKSIYNANEAIQLDAELYNESYVLVNDVDATCVVKGDNQ
                     KEYTYTFDKTINAYTLNAGILPVGNYTVSAAAHYKGKKNTAACQFSVRPIIAESLNTQ
                     ANHGLLQDIATQSGGKFYYPKDMQHIIDDIEKNDKVKSILYDSFNTQPLIDKKILFFI
                     ILLLLISEWFIRKYNGNI"
     gene            25738..26349
                     /locus_tag="PLL59_02360"
     CDS             25738..26349
                     /locus_tag="PLL59_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22674.1"
                     /translation="MQRIIKIAIIVFLSTQSINMFAVEKGDFHVNLTTNLGHHSFIPT
                     YQLKHYNAAKSKFEKLGFIPGVTFNMDFAASPYFSIGGWFTFSGKQFTKDSSSVLFDL
                     KYRSFGIGVRGVFHLYQLISEKGNAKLDADKFDVYLPLAIGGGFRLKSKESSLPEYKK
                     FKGGAIVGAGIGMTYYFVEHIGANLEAGYCEGSYAKIGLAFKF"
     gene            26541..27476
                     /gene="mdh"
                     /locus_tag="PLL59_02365"
     CDS             26541..27476
                     /gene="mdh"
                     /locus_tag="PLL59_02365"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HMY22675.1"
                     /translation="MKVTVVGAGAVGATVADNIARKELCHELVLLDIKEGVAEGKSID
                     MMQTAALLGFDTTITGSTNDYTKTANSDVVVITSGLPRKPGMTREELIGVNAGIVKGV
                     AENILKYSPNTIFVIISNPMDTMTYLALKATGIPKNRIIGMGGALDSSRFRYYLSQAI
                     GCSPSQLNADVIGGHGDTTMIPLTRLATLGSTPVSELLDAETLAKVAADTMVGGATLT
                     KLIGTSAWYAPGAAGMAIVESILRDEKKVITSCVYLEGEYGQTDICLGVPVVIGKNGW
                     EKIINYKLNEEEQAAFNKSADAVRAMNDVLKTLSI"
     gene            27582..28277
                     /locus_tag="PLL59_02370"
     CDS             27582..28277
                     /locus_tag="PLL59_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22676.1"
                     /translation="MKFAGKLLFSICIFLSACKKNDTVIVQFHITQTKHTPNNRSYDL
                     DLSKLKILFSNFTLIDVNGKSRVVKDIFLYGNGNNNFSFKMPKGNYTAFTFALGVDAA
                     TNNLMPASFPPSHPLSVENGLYWDMLKYRFIVAEGNIDDSALKNEIPDAPFSMHLGSD
                     TLYTIINASAIPYDGTSVFINLNLDKLFVLDSDPFQITNFSNHSEASEIAKGIAIKDS
                     FVDGITTSVVLPE"
     gene            28277..29353
                     /locus_tag="PLL59_02375"
     CDS             28277..29353
                     /locus_tag="PLL59_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMY22677.1"
                     /translation="MRKFFISCIGGLLFCSLLFHACNKVETYTPTPYIAPNIQNFRPL
                     PIDANNPMTVEGIALGKKLFFDPILSRDSSISCASCHKQEHSFSDDRIRSIGVDGLPG
                     ARHAMPLVNLAWQKDRFFWDGRTSSLPEQALKPIEDPLEMHIALPEVVRRLQNNSEYV
                     DLFWKAFNTKIITKELMAKAIAQFENTLISYNSKFDKYARGEVALTDAELRGFDIFKT
                     EKGDCFHCHSTSAPEVFISPDRTFANNGLDSVSSALNFIDFGLGKFTGNMADNGRFKI
                     PTLRNLAFTAPYMHDARFATLDEVIDFYNDGPKGSPTLEPIMREKANKRLETQFHWGL
                     NLSATEKADLKAFLLTLSDSSFIQ"
     gene            complement(29363..31513)
                     /locus_tag="PLL59_02380"
     CDS             complement(29363..31513)
                     /locus_tag="PLL59_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY22678.1"
                     /translation="MAKLPKHIKSKNEVIRKATPRLQNVSRINFNFEKWLPIIIAAFA
                     FILYANTLTHDYVLDDETVMAKNSMVTKGIKAVPEIMVTAYRAGAQDRQENLYRPLSV
                     AMFAVEWQLSPNQAFLGHLINVLLYALIGFLLYKLLRLWLPTKHSFILFAIALLFVAH
                     PLHTEVVANIKSRDEILGMLFGILSLQFFFYYANDKNILSIKNLLLGLLFFFLAMLSK
                     ESTITLFAVIPFSIYFFTLQHDFKKIFIAITSLTIAVIAYFILRHISIGGLVTFNEVS
                     ILNNSLMGVSSPVDRFATAIVLIGYYLKLFFFPFPLSFDYSLNTIPNASLSDVRFLLT
                     MLIIIAMAAYVLLQLKKKDIIAFSLIFFATTLSVVTNIFVLIEATLAERFMFLPSLGL
                     CMALILLLEKYLHYFAEADYSLLSIVKQNKLFTSLVFAFLLVFTTKSFLRNFDWKDNY
                     TLFSIDKDHNPNSYRTLSAYCNALFVEKIKPLQDGEQQKLDFCREAIFYTHKSLSITQ
                     DNFSAWSLLSYCYLQIKDYPNAIAAFENGIKFYSTKAPALDNFYMLGANAYYYSGNLQ
                     QAVSTAKAGLAINDTNAMLLNTYGMVLTETNDLKTAKMALEKSIQLDSSNADTWYNYG
                     NWYAKYNDFQTALNCYKKIESRNAATLTKINAYNNSGNCYAVLKQYDNAISYYKKVLT
                     LDAKNKSALQNLVLTYNNIGQPDVAASYQAQLNQ"
     gene            complement(31531..32058)
                     /locus_tag="PLL59_02385"
     CDS             complement(31531..32058)
                     /locus_tag="PLL59_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMY22679.1"
                     /translation="MKTKQEIKIELNAVFAQLTELVNTTDANRFKKSKNGKWNIAQNI
                     DHLTLSNNITMMALHTPKMALKQLYNTNNRTNWNYEEVVWKYQLQLNTGAKASLPFVP
                     KLGYIPVKSILVRLWKLSCVALLKSLDAWSENDLDTYIIPHPIIGKITARELLYFTIY
                     HCKHHLQTMQHLASA"
     gene            32085..34550
                     /gene="priA"
                     /locus_tag="PLL59_02390"
     CDS             32085..34550
                     /gene="priA"
                     /locus_tag="PLL59_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HMY22680.1"
                     /translation="METFHTYNIYVQVILPLSLPKLYTYAVPISLESEIQIGKRIEVQ
                     FGKQKLYAAIIYSISTSAPTAYVPKELLAVIDEQAIVSQEQLNFWTWIANYYMCNIGD
                     VMQAALPAYFKLSSDTYFVKNPNYEGDILALDDDEYQIASAFEHQEEITLNDIFAILQ
                     KKTVSKPVKNLMAKKIIYVKETLHEKYTPKFDTFVLLNTEISNNKQLLKATFDSISKY
                     PKQEILLLAYLDLSKQKEYIKRAELLKKADVSADTLKSLVKKNIFTLEERIVDRIHTK
                     DAEQLNTYELSIEQNQALVSIHNYFLEKEVCLLQGITSSGKTLVYIDLIKEQIAQGKQ
                     VLYLLPEIALTAQLVQRLEKTIGAVAVYHSKYNNAERIEIWNKVLRNESKIILGARSS
                     VFLPFQNLGLIIVDEEHDTSYKQYEPAPRYHCRDAAIYLAKVYQAKVLLGSATPSIES
                     YANAKAGKYGLVKLNNRYGDVQPPQIQFISLNEAHKKKEITTGITYLLRDEIQKTLDK
                     KEQVILFQNRRGYAPYIACSNCNWIPQCKNCDVSLTYHKYTDDLRCHYCGYTQTVVRK
                     CGACGTPELQQKGLGTERIEEDLKIMFPSAKIGRMDYDTVKSKHGHQKIISALEDGEL
                     DILVGTQMVTKGLDFNNVSLVGVLNADALLYYPDFRAIERAYQLLMQVSGRAGRREKR
                     GNVYIQMGNVHHVIVDYILEDNFEKFYSTQLQERMQYHYPPYTRLIQITLKHKDIKNT
                     IEAANKLYTYLDTKFQGWVIGPNAPIIQKVNNYYIRDILIKILRDTKELSRIKRDIQQ
                     AIEGLYQFQSFKQLRITIDVDVY"
     gene            complement(34567..35025)
                     /locus_tag="PLL59_02395"
     CDS             complement(34567..35025)
                     /locus_tag="PLL59_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMY22681.1"
                     /translation="MKRIEDEIKQKQFQNIYVKTDVNLLYTSGWLQNEKARFFKRFDL
                     SMQQFNVLRILRGQHPRPVMLNQITERMIDKMSNATRLVDKLNQKGLSTRELNPNSRR
                     QVDICITEKGLELLKEIDTAMNEQFPPAQYTNITEAELEQLNHILDKIRS"
     gene            complement(35031..35342)
                     /locus_tag="PLL59_02400"
     CDS             complement(35031..35342)
                     /locus_tag="PLL59_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HMY22682.1"
                     /translation="MENNNNQNQINIELPEDIADGEYANLAIITHSNQEFVVDFVRLT
                     PGVPKAKVKSRIILTPQHAKRLMMALGENVRKFEQAHGEIKEFEQIAFPMNFGPPTTQ
                     A"
     gene            complement(35442..>39517)
                     /gene="rpoC"
                     /locus_tag="PLL59_02405"
     CDS             complement(35442..>39517)
                     /gene="rpoC"
                     /locus_tag="PLL59_02405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HMY22683.1"
                     /translation="DRCGVEVTEKKVRRERMGHIKLVVPVVHIWYFKSLPNKTGYMLG
                     LSSKNVEAIVYYERFVVVQAGITEDGVPVNGESKKTKYLDLLTEEEYLDILDALPKDN
                     QLLSDDDPNKFIAKMGGDAIHALLSRIDLKKLSGELRHKASHESSQQRKSEALKRLSV
                     VEAFREANEHVENRPEWMVVQYLPVVPPELRPLVPLDGGRFASSDLNDLYRRVIIRNN
                     RLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKAEGGRALKSLSDVLKGKQ
                     GRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPKDMAAELFKPFIIRKLLERGIVKT
                     VKSAKKLVDKKEAVIWDILENVMKGHPVLLNRAPTLHRLSIQAFQPKLIEGKAIQLHP
                     LVCTSFNADFDGDQMAVHVPLSNASILEAQLLMLASHNILNPQNGTPITIPSQDMVLG
                     LYYLSKGKKTVGDDKVRGEGKVFYSAEEAIIALNEGQADLHANVKCRVRVRENNEFKY
                     KVIDTTVGRLIFNEVVPEECGYVNDILTKKNLKNVIGDVLVHTNIPTTVKFLDDIKEL
                     GYRQAFKGGLSFNIDDLIIPEVKQKLLTSATEEVDEVWSNFNMGLITNNERYNQIIDI
                     WTRVDTRITETLMKEISSHKQGFNSVYMMLDSGARGSKQQIKQLGGIRGLMAKPRKSG
                     SSGGEIIENPILSNFKDGLNVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQD
                     VVINGVDCGSLRGLTTTALKENEEVIESLFDRIVGRTALLDIVHPLTGEVIVKDGDLI
                     LEAQAAIIEASPIESVDIRSVLTCEMKRGVCAKCYGRSLTNNRPAQFGDAVGIVAAQS
                     IGEPGTQLTLRTFHTGGTAASVATESQMLAKFDGKLEFDSIKPVKQEVEGGEVRNVVI
                     GRTGEINIIDEATDRVLITNNIPYGATLAVKNGQKVKKGDLICSWDPFNNVIISEFKG
                     AIKYENVIEGITYREELEEQTGHREKVTIESRDKKKSPSLLIVDEKGKELYSYSIPVG
                     AHISVEDGQEIIPGAIIVKIPRILSKVRDITGGLPRVQELFEARNPSNPAKVSEIDGQ
                     ATFGKIKRGNREVTITSKDGVQRKYLVPLTKHILVQDGDFVKAGMPLSDGAITPSDIL
                     KIKGPSALQAYLVNEIQEVYRLQGVRINDKHIEVIVKQMMSNVLISDAGDTKFLEGET
                     VNKFDFIEQNDHIYDKKVITEAGDSEQLVVGQIVTLRQIREENSFLKRNDRKPVEARD
                     AIPATASPMLQGITRASLGTHSWISAASFQETTKVLNQAAINGATDLMRGLKENVIVG
                     HRIPAGTGVREFERVSVSSKKEYERMMAKKSMIEDEE"
BASE COUNT        12897 a         7058 c         6571 g        12991 t
ORIGIN      
        1 tgcaaaattt taaaaatggt gccattcgtg tgttagtagc aacggatatt gcagcgcgtg
       61 gcattgacat tgatgaactt tctcatgttt ttaattttga attaccagaa gtaccagaaa
      121 cttatgttca tcgcattggc agaactggta gagcaggaaa ctcaggcatt gcgctttcat
      181 tttgtgatgt tgaagaaaaa ttgctattgc gcgatataca aaaactcatt ggcaagcaaa
      241 ttcctgtaag aatcgaccat ccttatttaa taccacaagc tatcttagaa ccgaaggttt
      301 ttcaaacttt accaaaggaa ttgcaaaagc aaaaaaatcc agcaccgcaa aagaagaaga
      361 aaaaatggta tagaaaacca agtgtcaaaa aataaaatca atgtatttga ctataaagca
      421 tgacgtggtg tttccatgcg cttcatgtct tcttcatatg catcttttac agcctgcgcc
      481 accttttcgg ctacactcat atcaagtgta gatggaatga ttttatcgcg actgagttcg
      541 gttacacagt cggcaatagc ataagctgca gcaactttca tgcgtggtgt tatgcgtctt
      601 gctctgattt ctaaagcacc tttgaagata ccaggaaatg ccaaaacatt attgatttga
      661 ttaggcatat cgcttctgcc ggttccgaca acgcctgcac caccttcgta tgctaaatct
      721 ggtgaaattt ctggaattgg attggctaaa ggaaaaacaa ttggtgatgg attcatcgac
      781 atcacatctt cttttgtcag ctgatttgct tgacttacac caataaacac gtcggcattt
      841 ttcaatgccg ttttcaaagt gcctttaata ttatttggat tcgtatgtct gagtatttct
      901 cgtttgaaat catttaagtc tgcacgatct ttatgtataa ttcctttggt atcacacatg
      961 atgatttgtt tcactggtgt acaaacgctt ttatcaattt caaagcacat caataatttt
     1021 gagattgcca aacctgcggc gcctgcgcca ttgatgacaa cattcaaatc ttcgattttt
     1081 ttgtttaata acttacatgc atttataatt cctgccaata caacaatagc tgtaccatgt
     1141 tggtcatcat gaaagacagg aatacctaat gtttgcaggc gctcttcgat ttcgaaacat
     1201 cttggcgatg agatgtcctc caaatttata ccaccaaaaa cgggtgcgat attgcgaata
     1261 gtttggataa tttcttctgt attttgcgta tttaaacaaa taggaaaggc atcaacatcg
     1321 gcaaattttt tgaataacat ggccttgcct tccattactg gaattgcagc ttctggcccc
     1381 aaattaccta ggcctaaaat ggctgaacca tctgatacaa cagcaacagt attcgcttta
     1441 atggttaaat cccaaacttt ttctacatca ttagcaattt ggcgaactgg ctcagctacg
     1501 ccaggtgaat agcataatgt caaatcatct tttgtttcta aactcgtttt taaagaaata
     1561 gaaattttgc ctttcagctt ttcgtgcaac gctaacgatt ccgaagaata atccttcata
     1621 aaaaaaattg tattggataa aatgtggaca gctaatatac ttactatcaa gcattatgca
     1681 tacaattatg acaacaaaaa acagtttgat aatatgcaaa tactgactta catcaaacaa
     1741 ttcagtttat ataaaaaatg tataacaaaa ccatgaaacg atatgaatgt cgttaatctg
     1801 ttattttgaa cgtcaaatta catgatttta tatttccttt tgatttattg taggtataaa
     1861 ccgtgtttgt ttttttatcg ctggtgtaaa aatcccaatt agcaatatca tcattaaaca
     1921 tcacatcaat atcttgtatg agtactttca ctttatcatt ttcagaaatc gcaaagatga
     1981 tattttttga atttggacca acggcaaaca tatgagtatc attaacatga gctttataaa
     2041 cactagtgcc accaattaaa atattgacac ctacatctgg cgcatccgtt ttaaaaaagg
     2101 cataatccca cgcttgacca cttctgttaa agtcgtttac ttcaatgtaa tcgatatcca
     2161 tcgtaaagat ttttactttt ggttttagta tctgaacttg cggtttcttt agtgtcgttt
     2221 tataagcgat atcatttcca tctttgccag aaaaaacagc agtgacatcg atagtatgtt
     2281 ttcctgaatc gagttgcaaa gctgcataag gcacaaattg tcgaattgtt tgattgtttt
     2341 ttgttgcctc tgtttctgtt aatgataaat atctttcaat aacaacattt cctgtttcgt
     2401 tgctaacact gttatagcct tttgccgatt ttattggttt atcattttca gaaaatgtgg
     2461 ttttaaaaac aaacgatgca ttcttcagca ccgtatcttt tggatttata tcttcaattg
     2521 gcgtaaggtt aaaagtataa ataatagtca tgccttttac gccatctttt gttacattag
     2581 gttctaccgt taatgcttta aaaagtgtta gttcagtggc ttggatagaa gtataaataa
     2641 gtgcaaaaaa gaagagcaaa aacaagcgat ggcatttcat attaatggtt ttaatgtaga
     2701 acatctaaaa tataaaataa attccagaag caatatttat tacattgcta taacacttat
     2761 taagaattga cgtcttatat taaatcaaga aattaaacaa cattggatta tcgtttaatg
     2821 gcacaaaatc aactttgtat tttttcatac gcttaataag taagtcataa tcgtctttat
     2881 attttaattc tatacctact aatgctggac cagattcttt gctgttcttc tttgtatatt
     2941 caaacaaggt aatatcttcg ttggcagaaa gtactttggt tacaaattgg cgcaatgcac
     3001 cggcgcgctg tggaaacttg atgatgaaga aatgtttcaa gccttcaaaa atcagcgaac
     3061 gctctttaat atcaggcatt ctaccgatgt cattattacc accactgata acacaaacaa
     3121 cgtttttgcc ttttattttt tctttataat aatctaatgc agcgatagac aatgcacctg
     3181 ctggttctaa aactattgca tcagaattat acagttgcat aattttcgta cacactcttc
     3241 cttctggcac caatactact tcttctaagt tttttttaca taattggaat ggaatttggc
     3301 cgacttgttt aacggcagca ccatcgacaa agttatctat ttgcgacaat gtcaccacct
     3361 tatttttttc aagagacact ttcattgcgg gcgctccaag tggttcgaca ccaactattt
     3421 ttgtatttgg tgaaaattct ttaaaataag taattaagcc agctgctaaa ccaccaccac
     3481 caattggcac gaacaaataa tctattttct ttttgccaat ttgttctaaa atttccatgg
     3541 ccaccgtgcc ttgtccttca ataattaaag tatggtcaaa tggtggaaca agtatttttt
     3601 tatgtgtgga aatatatttt tgagcagcac ttttcgcatc gtcatacgta tcgccagaca
     3661 atattacctc aataaaattt ttacctattc tgcgtacggc atctattttt tgtcgtggcg
     3721 taggtgacgg cataaaaacg acaccatgaa tttttagcaa ctgacaagaa taggcaaatc
     3781 cttgtgcatg atttccagca gaagcacaca cgacacctaa ttttttttgt tctttagtca
     3841 attgagaaat aaagttatat gcaccacgta ttttataaga acgcacgact tgtaaatctt
     3901 ctcttttgag gtacactttc gcgccagttt cgtcagacaa aatttggttc aatttcagtg
     3961 gcgtcacctc aatgatacct tctaaatttt tctttgcttg tataatgcct ttcgtttttg
     4021 gtaattccga tgtcatagtg cgaaaatagg aataacttgc gaaataaaag ctattgtaac
     4081 aaacgaacta caatagaatt ctcttaatcg ttaaatttgt ttaatattat tgacatatac
     4141 actatttttt ttgaaatgat gttcaattat tatgaataaa gctggcatca ttagtattct
     4201 aacatatctg atttttataa gcaacatttc atttgcagtt gaatccgaca caatagctgc
     4261 gacaataaaa attgacagta tttatgtaga taaatcagac agagtgctta ttgtattttc
     4321 gaagcaacaa aaaataaaat cttattcgat tggattagga tttcaaccca ttggcaaaaa
     4381 acaatttgaa ggtgatggca aaacacctga aggcttgtat tttattgaca cgaaaagcgc
     4441 tcaaagtaaa ttccataaaa acctaaatat ttcttaccct aataataatg ataaagcata
     4501 tgcgaaatta caacaaaaaa atgcaggtgg cgatatcaaa atccatggct tgccaaatgg
     4561 tgtggaagat aagcattacg aagtaaatga ctggacttta ggctgtattg ctttaaggaa
     4621 taaggaaatt gacgaacttt ttaaacatat tcgaatagtt tgcccaatct atatcgctcc
     4681 ttgattatgt aggtctatta caacaagcca aattgtgaaa aatggtgaat gaaatgttta
     4741 tattgaaatt gcaaccagcc tgttttatct aaaggtccgt agaatggatg cgtagcattt
     4801 gcaaatgttg ttgcattaac tgatgccaag aatttaacaa tggtatcttt taatagttgt
     4861 ttggattgta ctatatcagc atacatcaag ggcagcgttt tgtcacttgg taaaaatgca
     4921 ggtttaaaat ttttaggcaa tccatattcc gatgccaaaa acgcaagctg tcttggtatt
     4981 ttttcctcag caatcatagt gctcatcggc aaagtgccga tagcaaaatt aagaggcaac
     5041 accagatgtt caagcatgtg atgcaccgtc atcaagcccc attttgcagg ttcatcgcct
     5101 tttatactat cgataattgc tggaattttg tgcgtgaaaa attcttcaaa atcaatttgc
     5161 atatctagta ttactctatc ttgtaatggc atacattagt attttgaatt tcctaaagaa
     5221 aaatctacag gcactttgcg tctgtttagc atatcttcga atgtacaaaa aaatccttgt
     5281 ttatagatgg gcattttata ataaacggaa tcttttgtat caatactttt aggtgcattc
     5341 ataaatgttt gcacatttaa aggcaaagag gtattgctgt tttgtgctag tatttttaaa
     5401 aaaataaaac agagtaatac aaagatgcaa tttgattttt tcacaacaat acaacaagat
     5461 ttttgcagaa aggttgttga aaaaaaatga aataaatata tcgattatgg atttaaaaaa
     5521 tcaccagatt caatacatga tgcatcagca ggaagtggca agttataaaa atagacccag
     5581 cattctaatt ctcctgcatc ggttttaatc ttcacttttg ttcgccgata ttcataaggc
     5641 ggctcgccaa taccgatgcc ttcatattca tcaatcttag accatgctgt atcattattc
     5701 aacacaaaaa catcgccaac aacttcatct gcttcatttg ttgtttcaat caatgctgga
     5761 taccaatcaa ttttatacag tttagctttc ggcaatattg ccaaaccata taaagacgcg
     5821 ttttctgcta aataagtagc aataggatgg ctttgattta aacgtaatgt accgtatgta
     5881 aataaatatt caggcatgtt acaaatttaa gatgttgttg tttatttctt ttattggcat
     5941 tttaataaaa aattatgaat taaaatattg cacctatggt aaactgaaaa aggtgtgttt
     6001 gtgttcgata attttcttta aaaaagcttc gcatgccgac actatatttt atatcgaaaa
     6061 taaacatggc aatatccatt cccataccta gctgatagct aaattgttca ggtctaaaaa
     6121 tcctgcgttt ttcttttttg ctcagtctat atatttcatt gttattttgt tcaatagttg
     6181 cctttatttt agtaatgaat gctgcctcga tgccattata gaatcgcaat ttatataaag
     6241 gatttttcac tgcataagcg ccaaaaataa aaggtattcc gactgtataa tattttaatc
     6301 tgagttgttc aaatggtata ttatttccaa tttccctatt aaaaacacca tttttcaaaa
     6361 acaccataga gcgtaacata ttcacttcta tttgcacaaa tgccatttga cgagatactc
     6421 ttaaaaataa gccaccttga taaccagcag cctcatctcg ttgagtgcca tttttatatt
     6481 tgaaatagtt aagattggcg ccaatttgac caccaaattg caatcgaatc ctgccgccag
     6541 ttttaaagaa aagtggtttt cgtttttgtt ggtaaatatc aacgcttgct gatgtgtcgt
     6601 tgttcgcaga aacatcaaac aataacatca acattattat agttaatatg gttttcattt
     6661 tattgttcaa catcttttac acatctaaat cctgtatgct ctaaacttgt ttccgcactt
     6721 gatttcatac gtgccgacac tcgaaatcct gaacaataac tatcattaca caagaacgaa
     6781 ccaccgcgca tcgatttttc taaggcagcg tttgctattt tattttcagg atttttaaaa
     6841 ttactaaatt ctacacattt gttataatat ttttcatcat accaatcgct acaccattcc
     6901 caaacattgc ccgccatgtc atacagttga ttcttatttg gaggaaaaga cataacaggt
     6961 gcacaacgtt caaaaccatc taacgctgta ttagaatatg gaaaatcgcc attccagcta
     7021 ttcatttttt tgaataaacc atgtctatcg ttgccgaaag tgtagagtgc attttgcagg
     7081 ccacatcgtg ccgccatttc ccattctgct tccgtaggca atcgtttgtt tgcccacttg
     7141 caatatgctt ccgcatcata ccacgacacc tgcaccaccg gaaaattatc tttgccttca
     7201 atagtagaat gtggcccttg cggatgtttc caatttgcgc cttccacaaa cttccaccat
     7261 gtatttggat tggcgacatc accttttttg attggattaa acactaaaga gcctgctttc
     7321 tgatgcacca ccacaccatt ttcatctttg aaattaaaat ctttttctgc tgttgttaca
     7381 taatttgtcg catcaacaaa ctgtttaaat gctgcattag tcacttctgt tttatcgata
     7441 aaaaaagcag aaatacgtac tttatgttta ggaaattcat cttggcgcgc ttgttgatta
     7501 ttgccgccca tttcaaaatc gccacctgca atcagcacca ttccatcttc ttgttgcatg
     7561 gtttgctgac aagatgtaaa aattataatt gtactaaaaa acaaaataaa atcaactaca
     7621 ttccttttac tcaggaaaaa agttgaaata agtatgttgt tctttttcat gagtaatgct
     7681 attagctcca tcgatattaa gcaggaaata aatacgattt accatctatc ataaagcggt
     7741 agtccatgat gcgtggccac ataggtttta ctggcaatgt tttttcccat tccatcaaat
     7801 cattttctaa atcagctgct ttttcaggca tgctaaagaa taagttatta cgttctcctt
     7861 tgtcttcttt taaattatat aattctttcc atttatcgcg tgtgctataa atcaatttat
     7921 aatctccttt tctgattgca tgtacatgat cagtgcgcca gtataattta tcgtgtggag
     7981 ctgtgtctat ttcttgtttg aaaaatggca ttagattaac accgtcatac actctatctt
     8041 ttggcaatgc aatattagaa acagcagcta ccgttgaaaa gatatccaag gaaataattg
     8101 gtttccgata aactacattt tctggcaaat gatttttcca tttaaaaata aaaggaatat
     8161 ttaaaccacc ttcataaggc gtaagcttac cgccttttaa tggttcatta cttgtaatgc
     8221 ctgaatacga tgccgagcca ttatcactca aaaagattat taatgtattg tcgtctaaac
     8281 cagcatcttt gattttctga tgaatggcac cgatagcatt atccatagag cgaataatcg
     8341 cattatagac ggctttcttt ttgtctttta catgctcaaa ttctttcaca tataaatcta
     8401 gtggcgcttg gtagggctca tgtggtgccg taaaaggaat gtataaaaag aaaggatttt
     8461 gcttattgtt ttcaataaac tgtatcgacc tatctctgat agcatctgtc aaatattgtt
     8521 tttcgtgttt tattttttta ttattttgat agatcattgc atcatcgcgg cgagccatgt
     8581 cccattgata ttttgcagaa aaagattgac gtctgtagtt gacaatatct tcttgaattt
     8641 gattatctac atacatagta aatgcgccat aacaaccgta ttggtaatca aatccacgaa
     8701 cagaaggaat attaaattta ggattttttc ctaggtgcca tttaccaata attgcggtgc
     8761 tgtagttcgc tttttttagt aattccgcta atgtaatttc tgttggtggc acgccttgtt
     8821 tggctatttg ccattcgcgt ggataacgag gcttggtggc aacgacccaa tttgtatctt
     8881 tttcggaaat atatcgccct gttagatact cgataatatt agttggataa tattctgttt
     8941 cttgtgtttc aaaaccgcag cgttgctgat atcgaccagt aagaatgcca cagcgcgatg
     9001 gcgcacaaac tgctgaggtg acatatcctt caacacattt cacgccttca ttggccaagg
     9061 catcaatatt tggtgtctgc accgtcgaat taccataaca gctaacatcg ttcaatccaa
     9121 ggtcgtcagc aacaataagc acgatattag gttgtttttc atttactaat attgtatcag
     9181 aaaggtatgc ttttttttgg gcgatttttt ctttatcaaa cttaatgcgc cagtgaatag
     9241 cacccgtaga aagtggatag catgcaacaa aaaatatacc tacaaataaa gataggataa
     9301 caggtataag caattttttg ttggacttta tcgacatttt gccttaattt tatagaagct
     9361 aaatttacca tgaaaatcga aataaaaaaa gaaatcgcaa atgcaattac acatggaatt
     9421 gggtttgtta catttgccac cttattgcca ttacttttta taaaagcatc gcactatgat
     9481 gtcaattata aattagtcgg tttaattttc tttgcgattg gcttggtagc tgtttattta
     9541 tcatctacga tttatcactc actcacaaac gagagagcca aatatatttt gcgtattgcc
     9601 gaccacatca gtatttacta cttaattgct ggcacctata ctgcattatt aatgcaaaca
     9661 attccattta ataaaattat gtggttcatt attattttat ggtctgttgt attaattggt
     9721 accataaaaa agatttttct aactggcaaa tacgatacga tttctaccat aatatatgta
     9781 gtaatgggct gcatgggttt atttcagata aatacaatta tgaaatttac accagacaaa
     9841 gtattgctct ttattctgct tggtggaatt gcctatctat taggtgtgat tttttatgct
     9901 tggaaaaaat tcacttacca tcatgccgtt tggcatttat ttgtattagc aggcagcatc
     9961 atgcatttct ttggcgtttg gtatgccata gaaaagccat agtcaatagc acaacaatag
    10021 cttccttttt atttctgttt ttaaaataga ttacttacgt aatatttgta cttttgttta
    10081 tgcacaaaac ggcaaataaa ggtatttttt attacattat cggcgattat ttagctgcac
    10141 ttttatcgtg gttatgtttt tttattttta gaaaactaca aatagaaaat gttacgcatt
    10201 ccatttcaag aatactacaa gatgaaaaaa taatacatgg cttagtgctt attccgattt
    10261 gctggctaat ctattatttt tatgacaata cctatacaag tttattaaaa aaatcgagat
    10321 tacatgaaat tcaacaaaca gctaaacaaa ctctaattgg aggaatatta ttgtttttca
    10381 gtttaatgtt aaatgatttt attaaaaaat acaatgatta ttattggttg tttgtaggtt
    10441 ttttagcatt tcattttttc actaccattt tattgcgttt acttatttta ggaatagtga
    10501 aaaatcgcat tagcaatgga aggcttacta taccgacttt attaattggt aattttgaaa
    10561 attgtcagaa agttgaacaa gaaattgagc attcaaaagt aaaattaccc tataaaattc
    10621 aagagaaaat tatcattgac aatgctaatc ttgacagtat cacgccaaag aataaagact
    10681 ttgaaaatgt catattagca tttggcacaa catcggcaca gcattttgaa aaaatcatat
    10741 ttcatttttt gaatcaagga attagtgtac aattattgcc cgatgaattg gatattttat
    10801 ctggaaaatt taaaaccaca agcgtctttg gctcgccatt attagaaatt ccgacaacgc
    10861 tattaaaacc ttgggaaaaa actagcaagc gagctgctga tatcacactt tcatttctag
    10921 gattattttt tcttctgcca tttttagtgt ttattgctat tcgcgtaaaa ctttcctcca
    10981 aaggaaacat cttttttatg caagaaagag taggattaaa tggcaacata tttcgaatta
    11041 tcaagtttcg ctcgatggta gaagatgccg aaaatggcac accgcgctta tcatctgaac
    11101 acgatgaacg catcacacca tttggtcgca ctatgcgaaa atatagaatc gacgaattgc
    11161 cgcaactttg gaatgtgctt atcggcgaca tgagcttggt tggtccacga cctgaacgtc
    11221 agtttttcat tgatcaaatt atccaaaccg caccacaata ccaattatta caacgtgtta
    11281 agcctggtat cacctcatta ggtatggtaa aatttggcta tgcatcgaat cttacggaaa
    11341 tgttacaacg catgcgttat gacttgctat acatagaaaa tatttcctta caaatggata
    11401 taaagattat gatttatact gtcattatat tgtggaaagg aaaaggaaaa taaggtttga
    11461 aatacaactc aatcaaccta ataaatatac taatccatta ttaacaggct catacatatc
    11521 aatgattttt ttcttgctac acatttgtgt aaacatatca cggataggtt taaaatcttc
    11581 gtgcattggg caaggtctat catcagagca atggtctaaa ccaagtccac attccttaaa
    11641 aacacgcata ccatcaatag attcaattac tttgataaca ggcaaatgca cctgattttc
    11701 gcttataaaa aaaccaccat ttggaccttt ggtgctgttt attatcttgt cttttaccag
    11761 tttttgcaac agttttccaa cggtatgttc attctcatct atttgttgtg caatctcgcg
    11821 aatgctcaat ttcttgtgtg attctttatt agaacctaaa caaacgaccg cttttattgc
    11881 tgctttacat gttaaactta gcatatatcc ttatctacct aaaaataatt ggccttcttc
    11941 tgaaatcata tctgcattcc atgttggctc atacgttaaa tttacatcaa ttccgtaaga
    12001 atcaaaagaa ctttgcaagg aattattaac atcttcaata atagactcac ccatagggca
    12061 aaattggctg gtcaatgtca tcacacaatt tatgcgtttt gcagtttcat caaacagtaa
    12121 actatatatc aatcctaagt ccactacatt taaaccaatt tctggatcaa ttaccgcttt
    12181 caaaccttct aaagcaatgg cactcaagtt ttcattgttt gtttcaatat gcatattata
    12241 caggtttatg ttttaataga ataaagacat tataattata tagtattgcg gtgcaaagta
    12301 gtccgactgc accgcatgaa atcaaaatta cgtttgacaa caaaattcca ctcagcaata
    12361 atacactcgc aattaaatag caaaaactca ttaatttaaa aatattatca tcaaataagt
    12421 ctttcggatt tggtgtttta ccgattcgag cacgatggtg atatacttta ttccatacga
    12481 taaatggtaa agtcttaaat gtcattccaa ggataataga tgttatccaa acaaagaaca
    12541 atacaaaacc ataagccgtt gccaatctta tttgatttac atttgaatag gtcattaaca
    12601 atagtagtaa aattaaaaat acaattggca tagccattat cataacagaa aaaacggaaa
    12661 gtttcatttg tgcatctact tgtttccgta ttctttgttg ataagcacgc aagcaataaa
    12721 acataaaaat aaatatgcca ctggaaacca taaaaattgg aaaagcgtaa taaatcaact
    12781 tagcagttaa aaaaaataaa actccaaaaa gtagcaagcc aagatttatg caagcataaa
    12841 tccaccaaag taagttggta ttattatact ttgaaataag aaacattgga attaaacgtg
    12901 atgcaacgcc aatgattagt aataaaaacc aacctgcaat tccgatatgt gcatgcaagc
    12961 tcaagtaatg caaggaatcg tatggcagca ccattgttgt aaaattaaaa agtaaaatcc
    13021 aacctaatat agcagtaagt aatagccaaa tgatggcagt aaatacaaaa atggcatgta
    13081 tattttcatg cttactttgc gtaatacttt ttccgatatt atataaaaat aatagcaaag
    13141 caaatatgat taacaagcct ccaccttgtg tgaaaacacc tacattgaat acataaaatc
    13201 catgtactaa tagtggaata ccgatagcca tcaaaacaaa cgtagcataa gcaagcttgt
    13261 tgccatatag ctcgccttca atcaatacag gcaccaactg atggcaagca ccaatgataa
    13321 tcatcgttgc ccagcctaat gccattgtgt gtgtaattgc taatatagtt ggatgaaaat
    13381 agtgtattaa aaattgtgct gatgagaaaa caagtaaaac agtagccaac aaaaacgaga
    13441 cggccgcata caaataaaat ggcaatacga ttttataaga tgtagttttt gcaagcgtag
    13501 tatttccttg tgcaacaaac atgctaagct ttaaagatta gtaaatgtac ttcattcgca
    13561 cttatttctt taacgcgata ttcaaatttt ctatcggcaa gttctggcaa caaaaaaaca
    13621 ggaatgcgtt tatgataaac aaataaagca ctatcacttg ctaaatgatc taaagccgat
    13681 aaaatagtgt gcataggcaa tggcatttct aagtcgcgca catcaattgt tgtcagtttt
    13741 tgttgatatt tttgaagaac atcgtcaaaa tctgatgcct gttgtatatc tacagtttct
    13801 acttttgatg ttgtatctgt tttatagaaa taggtatgaa taaggttttc atttattgtt
    13861 tcaacgactg ctataaatcc ttttttacgc aatatagcaa taagcggtgt tggctcaaaa
    13921 gtattgataa tctgaagcac ttcatctgat tgtaatttgc caatatgctg cataatcaag
    13981 ttaaatggat cgataccatt ttccaactct tgtcgcacat ctaatgcgac caatttttta
    14041 ttggctaatg cttgttttat atcttgttta tctgatgttt gcgtgttgat tgatgcttga
    14101 tttatttcct caatttcgaa accaattttc gataaaacag tataaaaatc ttcgactgta
    14161 cattttccta tttttgctgc atcttcaata gtaactcgcg atgctaaaat ttttcgaagt
    14221 attggatttt gtaatttttt gaaatgagga ttgatggctg caatctcatc aattgcggat
    14281 ttattatgtg ctataatagc cgatatcttt gtggacttat ttattttcat cttctttttt
    14341 ttcagatata caagaaagct aaaaaataat tcattaaaaa ccacctctgg aaatggcaac
    14401 acctaagaag gcaattgcca gcgatagcat caagtttatt cgacctgacc agcgtgcaat
    14461 tattcgaaat tttgtactat tatcaatacg catttggtcg atggctttat tgccaatata
    14521 aaaatcatga accgtaacaa tggttaacat gcatgaaaac aatagcaatt ttgtaccaaa
    14581 taatttacca tatgtatcgg tggccaagta tgtaatacta aatggtattc ctttggcata
    14641 catattatat gcaccagtca atatcaaacc aaaaatcgac atccagccgt acacccgaaa
    14701 tttaattcct gtggcataca agatgtcgat tttattgctt acttgtttaa tacttggcaa
    14761 tagcacaagc ggtagaaata acattcctcc tatccaaatg gcggcaaata atatgtgtaa
    14821 ataaaccgat attatatagg aaattcccat ttatgctagc aaggtttttt ctaattcttg
    14881 tgcttttgga aacaagatat tattttctaa gtgaacatgc aaatgcaaat cctcttcgaa
    14941 atcggacaat aatttatata agagaccgta gctggcgcaa gcatcttgtg gcaattgata
    15001 atcattgctc aaggtgcgaa tttcttccat tatttttcca gcattttcat gctccatttc
    15061 catcattgta attggacttt taaccgagcc gaatgatgct gccttcaaag gtgaaagcgt
    15121 attttttgcc attaccatag cttttatata aggaaataat acattttctt ctttgtccat
    15181 gtgcgattca aattctgcgc taatttcttc aactaatgcc tcaatgcgcg ctagttctga
    15241 gtgttgtgca ccatgaactt ttgcaacttt agcggcatac attttcatat ctggtaaggt
    15301 attttttaca aatgcatgat gtgtttgcac gatataatct gctaaaaaat ccaagctcca
    15361 ttcgttataa ggcaatgctc gtccagcaac agttctgcta gcttgcaaca attcagtttc
    15421 taccaaagta acgtcgatac ctttttcggc gcaagcttct tttactgttt ttttgccacc
    15481 acaacaaaaa tcgaggccaa attttttgaa tatttgtgcc ttacgcaagt ctttagttac
    15541 tatctctcct aatgtttcgg ctgcttctcc tgttttgttt tttccaatac gtaccttcca
    15601 ccaaattgga ccattttcaa gatattccca cgaaaaaaca ttgcctcgtt cgcctaacaa
    15661 ttgatagtat aaaggtttgg gatcatggtc attgtgtata gtcaaacttt ctccttctag
    15721 taattcgtcg aagcgagcaa atatagtcgg atgtttcatg cgaggctcta atagcgttac
    15781 atttaataca ttttctgctg tagtattcat aatacaagtt ttatttgatt tataatacac
    15841 aaaattaaaa cattcacgca ataaaagtat ttaattactt ttattattgt gttatgaatg
    15901 ttagtttcta tactgaattc tagttagaaa aaaactaaat atagtcgtta aattacagat
    15961 tatagtcgcc tgctgcttct ggctgataaa taatttcgtc aattctaatt ttagtcaagc
    16021 cagctggtac tatccattca atctcatctt tcactttata gccaattaat gctgttgcaa
    16081 taggtgaaaa aatagaaatt ttattttctt tcatattagc ttgttctgga tatacaatct
    16141 ggaattgaat ttgtctatcg ttatttaaaa agcttagttt cacgatagaa ttcatcgtta
    16201 ctacattaga aggaatagct tctggctcaa caatttcagc agaatccaat tctttcatca
    16261 gtttatctga ttctgattta tttatcgatt taaaatctct cgcatcgcta atactttttt
    16321 taatgcgtgc atagtccagt ctgtttacaa ttaatcggct catcattcac ttatttacta
    16381 ttttataata ttgattaaat ttaatgaaat ttgtttggaa ttcaaaaaat caaaaatgca
    16441 gcaaaaagca agccaaagta ttaaaaatca ttataatgca gataaaaaag aggcaaatta
    16501 aactttgcct cttaaaatgt gataactatt ttctaattgt tatttttaaa ttgctgttct
    16561 cgctcagcat ctttatcaaa tgctttgata atatctttta ctaatcgatg gcgaacgaca
    16621 tcatctgctg ataaatgtat catgccaata ccttcaattc catctaaaat ttgcatcgtt
    16681 ttacgcaagc ctgattgttg gtttttaggc aaatcgattt gtgtcatatc acctgtaatg
    16741 atacattgtg cagatggacc gatacgcgtt aaaaacattt taagttgtaa agtagttgcg
    16801 ttctgtgctt catctaatat cataaatgca ttatctaaag ttcgaccgcg catataggcc
    16861 aatggcgcaa cttctatgat tctattttgc atataataag caagtttatc tgctggtatc
    16921 atatcatcca atgcatcgta aagcggtcgc aagtaaggat ctattttttc tttcaaatca
    16981 cctggtaaaa aacctaaatt ctcgcctgct tccactgctg gacgagtaag gattattttt
    17041 tttacagatt tgttttttaa tgcacgcacg gctaaggcaa cggctgtata tgttttacct
    17101 gtacctgcag gtcccatcgc aaacaagatg tcattatctt cagagagatc gaccattttt
    17161 tgttggtttt cagtacgcgc acgcaccagc gttccgttgt tgccatacac aagtacatta
    17221 tcttgaaact gcgggaaatt agtttcgtat ggattgcgac catgtaatat ttcttcgaga
    17281 tgctgatccg aaatcttacc atatcgttct agatattcta cgagctgatg cactttgtcg
    17341 ctaaagactt ccaaatcttc ttgagaaccg gcaacgataa ttttgttgcc tcttgcactg
    17401 atattaagcc tgttaaattc ttttttcagg attttcagtt tgttgttgtt gaagccgaag
    17461 aattctaccg ggtcaacagt ttctagttta atcgttagtt cgctcaatta ttttatattt
    17521 ttgtttccag atatttagcc ttacattcgt agtctagata tcaataattt tttatcaatt
    17581 tgaaaataac ataaattttt aaatggcggt aataacgtta ttaacagatt ttggagaaag
    17641 agactattat gttggcttat ttaaaggtga tttatataca tcgagcccat ctgctaaaat
    17701 tgtggatatc tcacattcta ttccacctta tgacattgta actgctgcat tttttttaaa
    17761 aaatgtatat gcacattatc ctaaaggtac tatccatatt gctagagtat tcgaacaagg
    17821 cttaggcacg caaaaaatat tagcagcaaa atatcaaaat tactttttta tagcacccga
    17881 caacggacta ctcacgcttg tatttgatga aaagccagat ttaattgttg aaatagacat
    17941 gaaacaagca aaactgcgta cgctggaaga atactattgt agagtggtca aagaaattac
    18001 atttaataac aatattggtt ctattggtgt agcaacttct aattatgtag aaaaacgtgc
    18061 gcttttgcca gttattgaag aaaatagact gattgggaac gtcatttatg tcgataattt
    18121 tggcaatgtg atcactaata tccatattga tgacatcaaa cgctttgata ctagcaaaca
    18181 aatgcgcatc aattatcgaa aagtagattt tattgatcgt gtagtagaaa actattcaga
    18241 tgtacctcgc tcttatagtt tggctcgatt taactccatt ggttatttag aaatatgtat
    18301 aaacggcggt aacgcatctg atttattagg attaaaaaat ggtgatgcta tacaaatttt
    18361 atttgaatga ttattagaat tgtaaaactt acaatgcgtg aagaagaagt agcgacattt
    18421 aaaaattatt tcgcaacagt ttgcgaaact atacgtgctc aagaaggttg tacaatgcta
    18481 caggcatggc aagacataca ccagccaaat atttttttca catatagtat ttggcataca
    18541 gaaacagact taaacaatta cagagattct gctttcttcc ttcaattttg gaaaacagta
    18601 aaaccatggt ttgctgcaaa agcagaagtt tggagttttg ataaaatcgt agatttacct
    18661 taattattga tgttttgaca caaaagtgat gaatacaatt tccttaaacg actattcaat
    18721 ttatttagac gacagcctac aatctttata ccgttttatc gaagaaaaaa attacagcgc
    18781 attatttgcc attgttgatg aaaatacgaa acaacattgt ttgcctatca tcagtgagca
    18841 tatagttggc ttacatatca tcgaaacgca aagtggagaa accaataaaa acttgtacac
    18901 ttgcgaaaaa atatggcaac aacttatcac aaaaaatgca gatagaaaaa tattaatcat
    18961 caacttaggt ggtggtgtta ttggcgacat gggtggcttt gctgcaagtt gctacaaacg
    19021 tggtgtagat tttataaata ttccgactac tttattgtca caagtcgatt cctctatcgg
    19081 aggcaagctt ggcatcgatt ttaaatatgg aaaaaacctt attggtctat tccaaaatcc
    19141 aaaagctgtt tttatctcat ctgttttttt taatacgcta ccacagcgac aattcatcaa
    19201 tggttgggca gaaattttca aacacgcact cattcaagat agcgcacaat ggaatattta
    19261 caagcaatat gatattttat cttgccatat ggatgagatt gtttttcatt ctttacttgt
    19321 taaaaagaaa gttgtcgaaa tagaccctta tgaaaaagga ttgcggaaag tcctaaactt
    19381 tggacacacc attggacacg ctatcgaggc ttattctttg gaacacgaat caacaccatt
    19441 gttacacggc gaagctattg ctataggcat gatttgcgaa gcctatttat ctgtaaaaaa
    19501 atgtggattg acagcagatg aactaaacga aatccaaact gttttattaa aacattttcc
    19561 taagcacgat atctccactt tcgatatgaa taaaataatt gaaagtatgc gcatcgataa
    19621 aaaaaatgaa ggtgatacca tcatggcagc tttgctttct gctattggaa atagtgtata
    19681 tgacattcca ttgactacag aagacataca agaaagcatt gccttctata ttcaataatt
    19741 aaaacaatat ttaaataaaa tttatggcaa ctattacttt taaaggaaac gaagtgcaca
    19801 cagcaggcaa tttacctgca attggcgaca gcttaagcaa ttttagttta gtagcaagca
    19861 atctatcaga aaaaacatta gctgatttta gtggcgtgca attaatactc aatatcttcc
    19921 caagcatcga cacaggaatt tgtgctgcat ctgtgcgtaa attcaatcaa gaagcagctg
    19981 ctctagccaa cacaaaagta ttatgcatat caaaagattt accatttgct ttaagtagat
    20041 tttgtggtgc ggaaggcatt gaaaatgtag agacattatc agcattcaga agccatgatt
    20101 ttgattatgt attaatggct gatggtccat taaaaggttt attaagcaga tgtgttattg
    20161 tagtagatgc agatggaaaa gttgtatatg cagagcaagt agcagaaatc gctactgagc
    20221 cagattatac cgctgcatta gcaagcttag gataattcct ttttcagata tcacctaatt
    20281 gtggtcgaat aattaattct tctacaaccg aaaactcaga gagttgcgta attgtataaa
    20341 ccaattccgc aatatcttct ggtttactaa atctgctttc tggcaaatcc gtgccttgcc
    20401 acgaatccgt gagtgtcgca ccaggtagta tagcagttac ttttatgttg tatggcttta
    20461 attcttcgcg cagtgtttta gaaaaaccta ataacgcaaa ttttgaaatg gaatagctac
    20521 caccattttc atatgccata atactagcaa tagagcacat attaatgata taaccacttg
    20581 cttgtcgaat aaaagtaggt gctaattttt ttgtgagata ataagcacta tatacattag
    20641 attgtagtgt tttttccaaa acatcgtcct cttcttctgt aatttttcca ggaataaaaa
    20701 caccagcatt attaattaaa acatcgatag ttttaaaatt agacaagaca aaatcagcaa
    20761 aattatctat ctcctttttt acagacacat cgcaaacaaa agtcaatata cttggacatt
    20821 caatttgaag ttgacgcaac ttattagcat ctctcgagca tactattacg gaatgaccat
    20881 tgttgaaaaa ttttgctgca atggacttac caattcctct acttgcacct gtaataacga
    20941 tattcatgtt gtaaatgtat tattttttat catttcactc atcacttttg gctaatcact
    21001 aaaaatattc tatttttacc aattcttagg aagaataata catggcagaa tacattcatg
    21061 cgtcaggcgg tcccttattt catataggta gatatatact aatggtgaag cgatgtttcg
    21121 ttaaacctga acgtgccgca atgtattgga aagaaacatt acgacaaatg gccaacattg
    21181 gcgttggttc tgtaatgatt attattttaa tctccttttt tatcggtgca gtttctaccg
    21241 tacaatttgc ctataacctt tcacaatcaa tggtaccgat gtattatgta ggttatgtgg
    21301 ttaaagaatc tatgatatta gaactagctc ctaccatttc ttgtttaatt ttagcaggaa
    21361 aagtaggctc taatctctct tcagaattag gcacaatgcg cttaacagaa caaattgatg
    21421 cgctcgaaat aatgggcgtt aacacgattt cttatttaat tggaccaaaa atagcagctg
    21481 ccatcacgat gattccatta ttagtggttg tatctgcttt gacaggtatt tggggtggca
    21541 tgcttgccgg cgatttaaca ggttatgttt ctggcgtaga ttatacgcaa ggcattttaa
    21601 tgaattttgt ggcaaaaaat atatacatca tgctcactaa ggcagctgta tttggtttta
    21661 tacttacttc tatttcttgt tatcaaggat tttatgctta tggcagttct atagaaattg
    21721 gtaaagctag tacaagagcc gtggtgctca gtagtattat ggtattaatt gccgatttcg
    21781 ttttggcgtg gatgttatta aattaaaata atgattgaag taaaagatgt acataaatca
    21841 tttggctcac aacatgtgtt gaagggtgtt tcgtcattat ttgaagccgg aaaaaacaat
    21901 ctaatcatag gcaaaagcgg ttcaggaaaa accgtattat taaagtgtat tgtaggctta
    21961 ctcaatccaa cggaaggcca tgtactattc gatggcaaag attatgtagc catgaatatc
    22021 gaacagaaaa atgaaatccg acgagaatta ggaatgcttt tccaaggtgg cgcactgttc
    22081 gactctttaa ccgttgaaga aaatgtacgt ttcccactag atatgtttac caaaatggct
    22141 atgtctgaca aaaaagacag agtcaatgaa gtattaaaac gagtaaattt agtcaatgca
    22201 aatgccaaat atccatcgca actttcagga ggcatgcaaa agcgtacagg tatcgcacgt
    22261 gccattgtga tgaatccaaa atatttattt tgcgacgaac ccaattctgg tttagaccca
    22321 caaacttcta tcgtcattga tgaattaatc atggaaatta cggaagaata taaaattaca
    22381 accatcacca atacgcatga tatgaacagc gtaattacca atggcgacaa aattattttc
    22441 ttacacgaag gcaataaaca ttgggaaggc gacaaaattt ccttgtttga gtcagacaat
    22501 aaagaattaa tcgactttat atttgcatct gagtttttaa aagaagccag agatgcaaga
    22561 cacaaaaaat tattatccaa tttatccaag taattatgag cattttagta aataaaaact
    22621 caaaagtaat tgtgcaagga tttaccggta aagaaggtac attccatgca acacaaatga
    22681 tagaatacgg cactaatgtt gttggtggtg taactccagg caaagaaggc agtacacatt
    22741 tagacaagcc tgtattcaac acggtagaaa atgcagtaaa agctactggc gcaaatgttt
    22801 caatcatatt tgtaccacca gcttttgcag cagatgctat tatggaagca gcagatgcag
    22861 gcatacaagt cattgttact ataactgaag gcatacctgt tcaagatatg ataaaggcaa
    22921 aaaaatacgt cacctcaaaa ggcgtgcgta tgataggtcc aaactgccct ggaattatta
    22981 cggcagacga ggcaaaaatt ggtattatgc caggatttgt tttcaaaaaa gggaaagtag
    23041 gcattgtttc taaatcagga actttgacgt atgaggctgc tgaccaagtg gtaaaagctg
    23101 gttttggtgt atctactgct atcggtattg gtggcgaccc aattattggc acctcaacta
    23161 aagaggcggt tgaattattt atgaatgatg aggaaacaga agccatcgtc atgattggcg
    23221 aaatcggcgg cacgatggaa gtcgatgctg ctatgtggtt aaaagacaat atgaaaaaac
    23281 ctgtaatcgg ttttatcgct ggacaaactg ctcctaaagg ccgaagaatg ggacatgcag
    23341 gcgcaattgt tggtggtgca aacgacaccg ctgccgcaaa aatgaaaatt atgggcgaat
    23401 gtggtatcca tgttgtagat tcaccagcat ttatcggtaa aaaagtagca gaagtcttag
    23461 caaaataagc actattcaaa catatttcaa aagcagtttg tatgcacaaa ctgctttttt
    23521 gttttattaa ctttgtcgcc aaataacttt acattatttt gtgtagaaat tacctatcat
    23581 tatagcaaac aatttatgaa tacaactttc attgcagcat atccttggtg gttcatattg
    23641 ttttgcattg ctttaggtgt actcttctcc tatattttat atggtaaaaa gaaattcatt
    23701 ttcaccgaaa atgaaagtaa atggtggaaa tatggtttag caacgctgcg ttttttatcg
    23761 acatcattaa ttgctttttt gttgctatcg ctgttaatta aatcaaaaca tacggaagaa
    23821 gaaaagccgc taattttatt attacaagac aattcatcat ctctacatat atcattcgga
    23881 aatttctcaa aagaaaaata tgccactcaa ttatcggctt tacaagaaaa aatcagagaa
    23941 aaatatgata tcatttctta taattttggc aatacattaa gtaattttaa aacacccaat
    24001 tacacagaaa aagaaacaga catctcaaat gctattgatg aattatatat gcgtcatggc
    24061 actcaaaata ttggcgcaat tatattagcc agcgatggaa ttttcacaaa agggaattca
    24121 ccaatatata ataaaaatgc attaacaatt ccattttata caattgcatt aggagataca
    24181 tcgataaaaa aagatgcttt aatcaaaagc attcgatatc ctgaaatcgt atatcttggc
    24241 gaccaattca cgataaatat acaaatcgag gccaaccact tacaaggaca aacaacagtt
    24301 ttagaagtga ttgcgccaga cggtcatatt attttatcaa agccaattga aatcacagaa
    24361 gatcgttttt ccatacaaag tgatgccatc gctgaagcaa caaaaccagg tattttacgc
    24421 tatcaaatta gattaaaaac acttactggc gagactatcg ttgaaaacaa tactgacatc
    24481 gcatatattg aagttttaga tggccgacaa aaaatattgc tattatacga tgcgccacat
    24541 cctgatgtga aagcgtttaa aaatgctatc gaacaaaata aaaattatca atttgaatca
    24601 gcagatatca aaaactacaa tgctaatatt gccgatgcca atctcattat tctacatggt
    24661 ttaccttcta ctgatgccat atccaaaatc aataaaatac aagaaatagc aaacagcaat
    24721 ttaccaatat tatggattct ttcagccaat acaaatgtgg cacaattaaa cagcattcaa
    24781 aaaatcgtac aaatatcagg tgcttcttta aatggcaatg atgtgtttcc catctatcaa
    24841 aattcatttt caaaattcac tattaatgca ccttcgctgc aaacaataaa tgcattgcca
    24901 ccattgttag caccttatgg cagatacacg cttgcctcaa ctgctgatgt attatttcgt
    24961 caacaaatcg gcactattcc gacagacaat ccattgatag tatttaacga tatcaacggc
    25021 aaaaagacgg gatttatttg tggtgaaggc atttggcgtt ggcgaatgaa tgaatatgca
    25081 caaacaaata catttatggc aactgatgaa ttgattaaca aatgcgtgca gttcttaact
    25141 gtaaagggcg acaaacgaag gtttcgtgtg catacaccaa aatccattta caatgcaaac
    25201 gaagccatac aactcgacgc agaattatac aatgaaagtt atgtattagt caatgatgta
    25261 gatgcaacat gcgtagtgaa aggagataat caaaaagaat acacatacac tttcgacaaa
    25321 actatcaacg cctatacttt aaatgctggc atcttacctg ttggcaatta cactgtttct
    25381 gctgctgctc attataaagg aaagaaaaat acggctgctt gccaattttc tgttcgacca
    25441 atcattgcgg aatcattaaa cacacaagca aatcatggat tattacaaga tatcgcaaca
    25501 caaagtggtg gaaaatttta ttatccaaaa gacatgcaac atatcattga tgatatagaa
    25561 aaaaatgata aagtaaaatc gattctgtat gattcattta acacacagcc actcatcgat
    25621 aaaaaaatct tgttttttat tattttgtta ctgttgataa gtgaatggtt tattcgtaaa
    25681 tacaatggaa atatttgata tttttgatgt aacttttaaa atcttaaaaa agtatttatg
    25741 caaagaataa ttaaaattgc aatcattgtt ttcttatcaa cgcaatcgat aaatatgttt
    25801 gctgttgaaa aaggcgattt tcatgtcaat ctaaccacca acttaggaca tcatagtttt
    25861 attcctacat atcaattaaa acactacaac gctgcaaaat ctaaatttga aaaacttggt
    25921 tttattccag gtgtgacctt taatatggac tttgcggcaa gcccatattt tagtattggt
    25981 ggttggttta cattttctgg caaacaattt accaaagaca gcagtagtgt gctatttgat
    26041 ttaaaataca gaagctttgg tatcggtgtt cgcggtgttt ttcatttata tcaattaata
    26101 tctgaaaagg gaaatgcaaa attggatgca gataaatttg atgtgtattt accacttgct
    26161 atcggtggtg gttttcgctt aaaaagtaaa gaaagctcat tgccagaata taaaaaattt
    26221 aaaggtggtg ctatagtggg cgctggcatc ggtatgacct actactttgt agaacacatt
    26281 ggcgccaatt tggaagctgg ctattgcgaa ggctcatacg ccaaaattgg attagcattt
    26341 aaattctaaa aacttctatt ttacaaaaaa atgctattcg tacgaatagc attttttatg
    26401 tatagacatt catgttaatg aattgttatg agtaattaat tttcatataa acctaagttc
    26461 atactactca ttacttctac tcattactta ttagtatctt agcaccgatt tttacatttc
    26521 taatttttaa atagtataaa atgaaagtta ccgttgtagg tgcaggagca gtaggcgcaa
    26581 ccgttgccga taatattgca cgcaaagaac tatgccatga attggtatta ttagacatta
    26641 aagaaggtgt tgcagaagga aaatcaatcg acatgatgca gactgctgcc ttattaggat
    26701 ttgacacaac aatcacaggt tctacgaatg attacacaaa aactgcaaac agtgatgttg
    26761 tcgtaatcac atctggtctt ccaagaaaac caggcatgac gcgtgaggaa ttaattggtg
    26821 taaatgcagg tatcgttaaa ggtgttgcgg aaaacatctt aaaatattca ccaaatacca
    26881 tttttgttat catctctaat ccaatggata caatgacata cttggcgcta aaagcaacag
    26941 gtattcctaa aaatagaatc atcggtatgg gtggcgcttt agatagctca cgtttcagat
    27001 attatttatc tcaagcaatc ggctgttctc catctcaatt aaatgctgat gttatcggcg
    27061 gtcacggcga tacaacaatg attccattaa cacgtttggc aacattaggt tctacaccag
    27121 ttagcgaatt attagacgca gaaacattag caaaagtagc tgcagacact atggttggtg
    27181 gtgctacttt aacaaaatta attggcactt ctgcttggta tgctccaggt gctgctggca
    27241 tggctatcgt agagtctatc ttacgcgatg agaaaaaagt aatcacttct tgtgtttatt
    27301 tagaaggcga atacggacaa acagacatct gcttaggcgt tcctgttgtt atcggaaaaa
    27361 atggttggga aaaaatcatc aactataaat tgaacgaaga agaacaagcg gctttcaaca
    27421 aaagtgctga cgcagttcgt gcaatgaacg atgttttaaa aacattatct atctaataga
    27481 taaactcttt atatttcaat aggcatttgc tttttgcaag tgcctatttt tttttatttg
    27541 ctttagcaac aataagcatg atatatcgtt atatttaatg tatgaaattc gcaggaaaac
    27601 tcctcttctc tatttgcatt tttctttccg cttgcaaaaa aaatgatact gttatcgtac
    27661 aatttcacat tacacaaact aagcacacgc caaacaatcg tagctatgat ttagatttat
    27721 cgaaattaaa aatcttattt tccaatttta cgcttataga tgtaaatgga aaatcaagag
    27781 tagtgaaaga tatcttttta tatggcaatg gaaataataa ttttagtttt aaaatgccaa
    27841 aaggcaatta tactgcattt acttttgcat taggcgtaga tgctgctacc aataatttaa
    27901 tgcctgcttc atttccacca tcgcatccat taagcgtaga aaacggttta tactgggaca
    27961 tgttgaaata tcgatttatt gttgcagaag gtaatatcga tgattctgct ttaaaaaacg
    28021 aaataccaga tgcaccattt tctatgcact taggtagtga tactttatat accatcatta
    28081 atgcaagtgc aataccttat gatggcacaa gtgtatttat aaacttaaat ctagataagc
    28141 tttttgtatt ggacagcgat ccctttcaaa tcacaaattt cagtaatcat agtgaagctt
    28201 cagaaattgc gaaaggtatt gccataaaag acagctttgt ggatggaatt acaacaagcg
    28261 tagtattgcc agaatgatga gaaaattttt cataagctgt ataggtggtc tattgttttg
    28321 tagcttgctt tttcatgctt gtaataaagt agaaacctat actccgacac catacatagc
    28381 accaaatatt caaaacttta ggccattgcc aatagatgca aacaatccga tgactgtaga
    28441 aggaattgca ttaggcaaga aattattttt cgatcccatt ttatcgagag actcgagcat
    28501 ctcgtgtgca agttgccaca agcaagaaca ctcgttttct gatgacagaa ttagaagcat
    28561 tggtgtcgat ggcctgccgg gtgctcgcca tgcaatgcct ttagtcaatt tagcatggca
    28621 gaaagatcgt tttttttggg acggcagaac aagctcattg ccagaacagg cattgaaacc
    28681 catagaagat ccattggaaa tgcatattgc attgccagaa gtcgtgcgta gattacaaaa
    28741 caattcagaa tatgtcgatt tattttggaa agctttcaat actaaaataa ttactaaaga
    28801 attaatggca aaagccattg cacaatttga aaatacactc atttcttaca attcaaaatt
    28861 cgacaaatat gccagaggcg aagtagcatt aacagatgca gaattgcgtg gcttcgatat
    28921 ttttaaaaca gaaaaaggtg actgtttcca ttgccatagc acttcggcac cagaagtatt
    28981 tatttcacca gacagaactt ttgccaataa cggattagac tctgtatcat ctgcattgaa
    29041 ttttattgat tttggcttgg gaaaatttac aggaaatatg gcagacaacg gaaggtttaa
    29101 aattccgacc ttgcgcaact tggcatttac tgctccttac atgcatgatg cacgatttgc
    29161 aacattagat gaagtcatcg atttttacaa tgatggacca aaaggctcgc caacattgga
    29221 gccaatcatg cgcgagaaag ccaacaaacg cttagaaaca cagtttcatt ggggactgaa
    29281 tttaagcgca acagaaaaag ctgatttgaa agcattctta ttaactttat ccgactccag
    29341 ctttattcaa taaaatattt aatcactgat tcaattgtgc ttgataactt gctgcaacat
    29401 caggctggcc tatattatta taagtaagca ctaaattttg tagtgcactt ttgtttttag
    29461 catctaatgt caatactttt ttgtagtagg aaatggcatt atcatattgt tttaaaacag
    29521 cataacaatt accactatta ttgtaagcgt taatttttgt aagcgttgcc gcatttcttg
    29581 attctatttt tttatagcaa tttaatgctg tttgaaagtc gttatatttt gcataccaat
    29641 tgccataatt ataccaagta tctgcgttgc tactatctaa ttgtatagac ttttctaatg
    29701 ccatttttgc ggttttcaaa tcattcgttt cagtaagcac catgccatac gtattcagta
    29761 gcatggcgtt cgtatcgtta atggcaaggc ctgcttttgc tgtagataca gcttgttgta
    29821 aatttcctga ataataataa gcatttgcac ctaacatata aaaattatct aaagcaggtg
    29881 ctttagtgct gtaaaatttg attccatttt caaacgctgc gattgcattt ggataatctt
    29941 taatttgcaa ataacagtag ctaagcaaac tccacgcgct gaaattatct tgtgtaatag
    30001 acaagctttt atgtgtataa aaaattgctt ctctgcaaaa gtctaatttc tgttgctcac
    30061 catcttgtaa tggttttatt ttttcgacaa ataaggcatt gcaataggca gataaagttc
    30121 tgtaactatt gggattatga tctttgtcta tactaaacaa agtataattg tctttccaat
    30181 caaaatttcg gagaaacgat ttagttgtaa aaacaagtag aaacgcaaac acaaggcttg
    30241 taaacaattt gttttgtttt acaattgaca acaaagaata atctgcttct gcgaaatagt
    30301 gtaaatattt ttccagcaac aatatcaatg ccatacacaa acctaatgaa ggtaaaaaca
    30361 taaaacgctc agccaatgtt gcttctatca acacaaaaat atttgttacg acagacaatg
    30421 tcgtagcaaa gaaaataaga ctaaatgcga tgatatcttt tttcttaagt tgtagcaaca
    30481 catacgcagc catcgcaata ataattaaca tcgtcagcaa aaaacgaaca tcacttaagc
    30541 tcgcatttgg aatagtattt aatgaataat caaaagaaag tggaaatgga aaaaagaaca
    30601 gtttcaaata atatccaatc aatacaattg ccgttgcaaa tctatctact ggagaactta
    30661 cgcccattaa agaattattt aaaatgctta cctcattaaa agtgactaat ccaccgatgg
    30721 aaatatgacg cagaatgaaa taagctatca cggcgatagt tagcgatgta attgcaataa
    30781 atattttctt aaaatcatgt tgtaatgtaa aaaaataaat agaaaatgga atgaccgcaa
    30841 atagtgttat cgtgctttct ttggatagca tggctaagaa aaaaaacaac aaacctagca
    30901 gtagattttt gattgacaga atatttttgt cattagcata ataaaagaaa aattgcaaag
    30961 acaatatgcc aaacaacatt cctaatattt catctctact tttaatattc gcaacaactt
    31021 ctgtgtgtaa tggatgagca acaaacagca acgcgattgc aaacaagata aatgagtgtt
    31081 ttgttggtag ccataatctt aataatttat acaataaaaa tcctattagc gcatatagca
    31141 acacattgat aagatgtcct aaaaatgctt ggtttggcga caattgccat tcaacagcaa
    31201 acatagcaac agacaatggt ctgtatagat tttcttgtct atcttgcgca cctgcacgat
    31261 aggcagttac catgatttct ggaactgctt tgatgccttt cgtcaccatg ctattttttg
    31321 ccatcacggt ttcatcatct aacacataat catgcgtaag cgtattggca tacaatataa
    31381 aagcaaatgc agcaataatt attggcagcc atttttcgaa attaaaatta atgcgagata
    31441 cattttgcaa ccttggtgtt gcttttcgaa ttacttcatt ttttgattta atatgctttg
    31501 gcaatttcgc cattagaagt ccgatttagt ttatgcagaa gctaaatgtt gcattgtttg
    31561 taaatgatgc ttgcaatggt aaatagtaaa atacaacaat tcacgcgctg taatttttcc
    31621 aattatcgga tgcggaatga tataagtatc taaatcattt tcgctccatg catctagtga
    31681 ttttaataat gcgacgcaag acaatttcca taaacgaacg agtattgatt ttactggaat
    31741 atatccaagc ttgggtacaa agggcaagct tgctttcgcg ccagtattta gctgcaattg
    31801 atatttccaa actacttctt cataattcca atttgtacga ttatttgtat tgtataattg
    31861 ttttaatgcc atttttggcg tgtgaagtgc catcatagta atattgttcg acaaggtcaa
    31921 atggtctata ttttgagcaa tattccattt gccatttttt gattttttaa atctatttgc
    31981 atctgttgta tttactaatt ccgtgagttg cgcaaaaact gcatttagct caatttttat
    32041 ctcttgcttt gttttcattt tagtattatt gtagtgtaaa aatcgtggaa acttttcata
    32101 catacaacat ctatgttcaa gtaattttac ctttatcatt acctaagctc tatacgtatg
    32161 ctgtaccgat ttcgctagaa agtgaaatac aaataggcaa acgaattgaa gtgcaatttg
    32221 gtaagcagaa gttgtatgca gcaataatct attcaattag cacatcagca ccgacagcat
    32281 atgtacctaa agaattatta gctgttattg atgaacaggc tattgtatcg caagagcaac
    32341 tcaatttttg gacttggata gccaattatt acatgtgtaa cattggcgat gttatgcaag
    32401 ctgcattgcc tgcttatttt aaattatcat ctgataccta tttcgttaaa aaccctaatt
    32461 acgaaggcga tatcctcgca ttagatgatg atgaatacca gatagcatcg gcatttgaac
    32521 accaagaaga aataacactc aatgacattt ttgccatctt gcaaaaaaaa acagtaagca
    32581 agcctgtaaa aaatctaatg gcaaaaaaaa tcatctatgt aaaagaaaca ttacatgaaa
    32641 aatacacacc aaaatttgac acttttgtct tgttaaacac tgaaatatcc aacaacaagc
    32701 aattactaaa agcaacattt gacagcattt caaaatatcc aaagcaagaa attttattat
    32761 tggcatatct tgatttatct aagcaaaaag aatacatcaa acgtgctgaa ctattaaaaa
    32821 aagctgatgt ttcggctgat acattaaaat cattagtaaa gaaaaatatt tttacattag
    32881 aagaaagaat tgttgataga atacatacaa aagatgcgga acaattaaac acatacgaac
    32941 tttctattga acaaaatcag gctttagttt ctatacataa ttatttcttg gaaaaagaag
    33001 tttgcttatt acaaggcatt acttcgagtg gcaaaacatt agtctatatc gatttaataa
    33061 aagagcaaat agcgcaaggc aaacaggttt tatatttatt acctgaaata gcattaactg
    33121 cacaattggt acaacgatta gaaaaaacca ttggcgctgt tgcggtctat cactcgaaat
    33181 acaacaatgc ggagcgtata gaaatatgga ataaggtttt gcgcaatgaa tcaaaaatta
    33241 tattaggagc acgctcttct gtatttctgc ctttccaaaa tttgggttta atcattgtag
    33301 atgaagagca cgacacgtca tacaaacaat acgaaccagc accacgctat cattgtagag
    33361 atgctgccat ctatttagct aaagtgtatc aagctaaagt gttattaggc tctgcaacgc
    33421 ctagcattga aagctatgcc aatgcaaaag caggtaaata tggcttagta aaattaaaca
    33481 atagatatgg cgatgtgcag cctccacaaa tacaattcat cagcctcaac gaggcacaca
    33541 aaaaaaagga aattacgaca ggcattactt atttacttcg tgatgaaata caaaaaacat
    33601 tagacaaaaa agagcaagtt atattgttcc aaaatcgtag aggctatgcg ccttatattg
    33661 catgtagcaa ctgcaattgg attccacaat gtaagaactg tgatgtaagt ttaacgtatc
    33721 acaaatatac agacgacttg cgctgtcatt actgtggtta tacgcaaact gtagtgcgta
    33781 aatgtggtgc atgtggtact cctgaattac agcaaaaagg acttggtacg gagcgtatcg
    33841 aagaagattt aaaaatcatg tttccaagtg caaaaattgg aagaatggat tacgacacag
    33901 taaaatcaaa acatggtcat caaaaaatca tcagcgcatt agaagatggt gaattggata
    33961 ttttagtagg cacgcaaatg gtgacaaaag gattggattt taacaatgta agtttagttg
    34021 gcgtgttgaa tgcagatgcg cttttgtatt atcctgattt cagagcgata gagcgagcat
    34081 accaattgct gatgcaagta agtggtagag ctggcagacg cgagaagcga ggtaatgtgt
    34141 atattcaaat gggcaatgtg catcatgtta ttgtcgatta cattttagag gataattttg
    34201 aaaagtttta tagcacacaa ttacaggaac gcatgcaata tcattatccg ccatacacaa
    34261 ggttgataca aattacacta aagcataaag acataaaaaa cacaatcgaa gcagcaaata
    34321 aattgtacac ttatttagat acaaaatttc aaggctgggt cattggacct aatgcgccca
    34381 tcattcagaa agtaaacaat tattacattc gcgacatttt aataaaaatt ctgcgtgaca
    34441 caaaagaatt atcgcgcata aaaagagaca tacaacaagc tattgaaggc ttgtatcaat
    34501 ttcaatcttt caaacaatta agaataacaa ttgatgtaga tgtttattag gaaaagcaag
    34561 agaaaatcag cttcgtattt tatccaagat atgatttaat tgttccaatt cagcttccgt
    34621 aatatttgtg tattgtgctg gcggaaattg ttcgttcata gcggtatcaa tttcctttaa
    34681 taattccaaa cctttttctg taatgcaaat atcaacctgt cttcgactat taggattaag
    34741 ttcgcgtgta cttagtcctt tctggttgag tttgtccacc aagcgtgttg cattggacat
    34801 tttatctatc atgcgttcag taatctgatt caacattaca ggtcttggat gttgaccgcg
    34861 aagaattcgc aaaacattaa actgttgcat agacaaatcg aagcgcttaa aaaaacgagc
    34921 tttttcattt tgtaaccaac cagatgtgta caataaattc acatcagttt tcacataaat
    34981 attctgaaac tgcttttgtt ttatttcgtc ttcaattcgc ttcatatcaa ttatgcttga
    35041 gttgttggtg gtccaaaatt cataggaaat gcaatttgct caaactcttt aatttcgcca
    35101 tgtgcttgtt caaattttct aacattctca cctaatgcca tcatcaaacg tttggcgtgt
    35161 tgaggtgtta aaataattct tgattttact tttgctttcg gcacgcctgg agtaagacga
    35221 acgaaatcca ctacaaactc ttggttagag tgtgtgataa tggctaagtt tgcatattca
    35281 ccatcagcaa tatcttctgg caattcaata tttatttgat tctggttgtt attattttcc
    35341 atatcaatga cttgtagtta gttaaacaaa tgtacaaaaa tagcgctata aaaaaaacag
    35401 gctgtcacga ggacagccta tttatttatt aaagaagttg attattcttc atcttctatc
    35461 attgactttt tagccatcat acgttcgtat tcttttttag agcttacaga aaccctttcg
    35521 aattcgcgaa caccagtacc tgctggaata cgatgaccaa cgataacgtt ttctttcaaa
    35581 cctctcatta agtcagtagc accattgatg gcagcttggt tcaatacttt cgttgtttcc
    35641 tggaacgatg ctgctgaaat ccagctgtgt gtacctaatg atgctcttgt aataccttgt
    35701 aacattggac ttgccgttgc aggaatcgca tcacgcgctt caactggttt tctatcgttg
    35761 cgtttcaaga acgaattctc ttcacgaatt tgacgtaatg ttacaatttg acctacaact
    35821 aattgctcag agtcacccgc ttctgtgata acttttttat cgtagatatg gtcattttgt
    35881 tcgatgaagt caaatttatt cacggtttca ccttctaaga atttagtatc accagcatcg
    35941 ctgattaata cattactcat catttgtttt acaattactt cgatgtgttt atcattgata
    36001 cgtacgcctt gtaagcggta aacttcttga atttcattta ctaagtaagc ttgcaatgca
    36061 gatggacctt taattttcaa gatatcactt ggtgtaattg caccatcaga caatggcata
    36121 cctgctttta caaagtcgcc atcttgaacg aggatatgtt tagttaaagg caccaaatat
    36181 ttacgttgaa cgccatcttt agaagtaatg gttacttctc ggttaccacg ttttattttt
    36241 ccgaatgttg cttgaccatc aatctcagaa actttggcag gatttgatgg attacgtgct
    36301 tcgaataatt cttgaacacg aggaagacca cctgtgatat ctcgaacttt acttaagata
    36361 cgaggtattt tcacgatgat agcacctgga ataatttctt gtccgtcttc tacagaaata
    36421 tgtgcaccta ctggaataga ataagaatat aattctttgc ctttctcatc aacaattaat
    36481 agagatggcg attttttctt atcacgtgat tcgatagtta ctttttcgcg gtgaccagtt
    36541 tgctcttcca attcttctct ataagtaata ccttcaatta cgttttcgta tttgatagca
    36601 cctttaaact cagagataat aacattgttg aacggatccc aagaacaaat caaatcacct
    36661 tttttcactt tttgaccatt tttcacagcc aatgtagcac cataaggaat attgttggta
    36721 attaatactc tatctgttgc ttcatcgatg atattgattt cgccagttct accaataacc
    36781 acatttctga cctcaccacc ttctacttct tgttttactg gtttgatgct atcgaattct
    36841 aatttaccat caaacttcgc taacatttgt gattcagtag caacagaggc tgctgtacca
    36901 cctgtgtgga aagtacgaag tgtcaactgt gtacctggct caccgataga ctgtgcagca
    36961 acgataccta ctgcatcgcc gaattgcgct ggtctgttat tggttaagct tctgccatag
    37021 cattttgcac acacaccacg tttcatttcg caagttaata ccgagcgaat atctacgctt
    37081 tcaattggag aggcttcgat gattgcagct tgtgcttcta atattaaatc accatctttt
    37141 acaatcactt cgcctgttaa tggatgtact atatctaata aggctgtacg gcctactatt
    37201 ctatcgaata atgactcgat aacttcttcg ttttctttta atgctgttgt tgtcaaacca
    37261 cgcaatgaac cgcagtccac accattaata acaacatctt gagctacgtc cactaaacga
    37321 cgtgttaaat aaccagcatc tgctgttttt aatgccgtat ccgctaaacc tttacgagca
    37381 ccgtgtgtag agatgaagta ttctaatacg ttcaagccat ctttgaaatt agataagatt
    37441 gggttctcga taatctcgcc acctgaagaa ccagatttac gaggttttgc catcaaacca
    37501 cgaataccac ctaactgttt aatttgttgt ttagaaccac gcgctccaga atctaacatc
    37561 atgtacacag agttaaaacc ttgtttgtgt gatgaaattt ctttcatcag ggtttctgta
    37621 attcttgtgt ccacacgtgt ccagatatcg ataatttggt tataacgctc gttgtttgta
    37681 attaaaccca tattgaagtt agaccaaact tcatctactt cttctgtagc ggaagtcaac
    37741 aatttctgtt ttacttctgg tataattaaa tcgtcgatgt tgaacgacaa accacctttg
    37801 aatgcttggc gatagcctaa ttctttgata tcatctaaga attttactgt agttggaatg
    37861 ttagtatgca ccaatacatc accaattaca ttttttaaat tctttttcgt taaaatatca
    37921 ttgacataac cacattcttc aggcaccact tcattaaata ttaagcgacc aacagtggta
    37981 tctattactt tatatttaaa ctcattattt tcacgaacgc gcacgcgaca ttttacattc
    38041 gcatgtaaat cagcctgacc ttcatttaat gcgataattg cttcttcggc tgagtagaat
    38101 acttttcctt cgcctcttac tttgtcgtcg ccaactgtct ttttgccttt tgataaataa
    38161 tataaaccta ataccatgtc ttgtgacgga atggtgatag gtgtaccatt ttgtggattc
    38221 aagatattgt gcgaagccaa cattaacaat tgtgcttcta agatagatgc attgctcaat
    38281 ggtacgtgta ctgccatttg gtcaccgtca aagtcggcgt tgaatgatgt acaaactaat
    38341 ggatgcaatt gtatcgcttt tccttctatt aattttggtt ggaatgcctg aattgacaag
    38401 cggtgtaatg ttggtgcacg gtttaataaa acaggatgac ctttcattac gttttccaaa
    38461 atatcccaaa taaccgcttc ttttttatct actaatttct ttgcagattt tacagtttta
    38521 acgataccac gctctaataa tttacgaatg ataaacggtt tgaataactc agccgccata
    38581 tcttttggca aaccacattc gtgaagtttc aactcaggac caaccacgat aaccgaacga
    38641 cctgaatagt caacacgttt acctaataag ttctgacgga aacgaccttg tttacctttt
    38701 aatacatctg ataaagattt caacgcacga ccaccttctg cttttactgc atttgattta
    38761 cgtgaattat caaataatga gtcaacagct tcctgcaaca tacgtttttc gttacgtaag
    38821 attacttccg gtgctttgat ttcgattaat ctttttagac ggttatttct gataatcaca
    38881 cgacgatata agtcgttcaa atctgatgat gcaaaacgac cgccatctaa tggaactaat
    38941 ggacgcaatt ctggtggaac tactggtaaa tactgcacta ccatccactc aggtctattt
    39001 tctacatgct catttgcttc acggaatgct tcaactacac tcagacgttt taacgcttct
    39061 gatttacgtt gttgtgaaga ttcatgtgat gctttatgac gcaattcgcc tgataatttt
    39121 ttcaaatcaa ttctagacaa taaagcatgg attgcatcgc cacccatttt tgcaatgaat
    39181 ttatttggat catcatctga taataattga ttgtcttttg gtaaggcatc taaaatatct
    39241 aagtactctt cttctgttaa aagatctaag tattttgttt ttttgctttc gccatttact
    39301 ggaacgccat cttctgtaat accagcttgt actactacga aacgctcgta gtaaacaata
    39361 gcttctacat ttttagaaga taaacccaac atgtaacctg tcttattcgg caaggatttg
    39421 aaataccaga tatgaactac aggcaccacc aatttgatgt ggcccattct ttcgcgacgt
    39481 acttttttct ctgttacttc cacaccgcag cggtcgc
//
LOCUS       DAOQQV010000011        39316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_284_length_39316_cov_7.309442, whole genome shotgun sequence.
ACCESSION   DAOQQV010000011 DAOQQV010000000
VERSION     DAOQQV010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 39316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39316
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_284_length_39316_cov_7.309442"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLL59_02410"
     CDS             complement(<1..403)
                     /locus_tag="PLL59_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22684.1"
                     /translation="MKKLSLLIAMGIACSIAIAKPTLNASTFETYSAQMQQMSEKYIA
                     AKQYGNANKTIDVWLSTYQSLSDKDKLANAARYAEIMYNRACSLTQTNEMFQALKALK
                     EAVKSGFNNRDKAENDPNLSKLKTYEEFGRIL"
     gene            complement(514..2322)
                     /locus_tag="PLL59_02415"
     CDS             complement(514..2322)
                     /locus_tag="PLL59_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HMY22685.1"
                     /translation="MIKTFNINLAGQIFNINEDAYEQLSAYFNSLRAFYANESDKDEI
                     IRDIEARFAELFLAKGKNYIVTKEDATTVINLMGNPQEFDEENSQNNSNNTSNNNSTT
                     ITEFTNQVGGKKLYRDFDNGLITGVCAGLSAYFGINDAIWLRLLFIGLTIIGFGSPII
                     VYIVLSLIMPKAETAAQKLEMKGEPINLSNIVKNINEEQIDVKSKGFFNRIIAFFGAG
                     IMLFFKALLWIGIACAILIGSIVIISLLITLVVISCLALFGIPVANSYFFTNTSDGWF
                     FGIGALLACIIPIIFSILAIVHVLSKTLKPLNKKTLFPLLGLFLFGILLLNISGYNAK
                     KLVQEKKKIIQSYPLNYAYQSDTLQIAMNPEIQDEDYDNININGVSDLLEFVSDHEDN
                     LFPVEIEVYPSRNDSFFVVKEYAANGKDMKDAVINATSFKHNIRQVNNKLLIDPYIQF
                     DNNKIKYRNQKLKIKVYVPEGKVIRWDERTEGYMNMGKIAINWEPIRNGSTIIPPVPP
                     LPPLPPTATKSSHKVVIKNGDTSSSSKIIINIEDDNGDITEALDKAQEKLDAARERLE
                     DANIRLDDSMQEIFENRLSREHYIFKMVNGELVAID"
     gene            complement(2359..2739)
                     /locus_tag="PLL59_02420"
     CDS             complement(2359..2739)
                     /locus_tag="PLL59_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HMY22686.1"
                     /translation="MTTTSSSAFSIENTKVQMRKGILEFCILGIISRGEVYASDILDA
                     LKNANMLVVEGTVYPLLTRLKNAGLLTYEWKESTSGPPRKYFTLTLEGQTALEELKQT
                     WNELSEAADKATHYSKPEADNISI"
     gene            complement(2792..3145)
                     /locus_tag="PLL59_02425"
     CDS             complement(2792..3145)
                     /locus_tag="PLL59_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22687.1"
                     /translation="MNNLIIAALTFVMLPALENNVTAKSIKSEKAAQKEIVVEKMDFA
                     SAFDKNTDLEKNYTPSVVDVDYIIEENGKAMITYINSDNQEAKENVVKFIESATYSQN
                     INPGKLYSMKLMITK"
     gene            3359..4969
                     /locus_tag="PLL59_02430"
     CDS             3359..4969
                     /locus_tag="PLL59_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMY22688.1"
                     /translation="MRRVNLSIIFIFFLQFLAIDVFSTDGEVNHSPYDVVYNHIHFLK
                     KGSYDELQSAASFDIPNKKNRIDAAITLKEILDAKIDPSALLNKIPDDPSFTDSTSKR
                     HIYVLYDKLPQVYVELKGNKWYYSQATVDALPQLHRKIFPFGTNIWAKWFPIKNNNEF
                     LRLYTWQWIGIGIILLAFILCFTLLKYLFRFIFHKILFKKYINEINDIDKLRTSANLF
                     SVWIGFKLFQLFIPTLFINPKYAMPLITGVNFVAASIIVLVVYKLVELIIFYSKNYVQ
                     NTATQWDDQIVMVLQKFLKFIVIFLGLFYVLNTLDVNIATIIAGLSVGGLALALAAQD
                     TVKNFIASVMIFIDKPFKIGDTIKGDNFEGAVQEVGFRSTRIKTAEDSLVYIANAKLS
                     EMIIDNKGFRVFKKYKTELAIAHATPLYKIEQFVAGIRTILLKYPYTKNSTIDVFLSN
                     IQSAGLSITLSYTYKVYSQREELQHREFILLKIIALAELLQIKLFETSPVVLANIDNN
                     KADENIESVKQKLDQFFTDLDTQISKTK"
     gene            complement(4993..9471)
                     /locus_tag="PLL59_02435"
     CDS             complement(4993..9471)
                     /locus_tag="PLL59_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY22689.1"
                     /translation="MQQSINWTLNGNVVSNTATLNESVCPTSNNNFIANLVNDRCDGS
                     TVVVSDNFNFAVNQKPTADAGPDKVIGCASPSVQIGTPAVAGHTYTWSPNSNLNNAGI
                     AQPLATANGTYTLTVRNTATGCTATDAVTVTSNNTPPVANAGPDKVLSCSGTTIGTAG
                     VAGNSYSWSPSTGLNDPNIAQPNVTLANTYTLTVTNIANGCITTDAVIVTGSILLPTA
                     DAGPDKEITCTNPTRQIGTAAVAGNTYSWSPTTGLSDPLIAQPIASLPNTYTLTVTKT
                     SNGCFTTDAVLVTRDIVAPTANAGADKILTCTATTQQIGTPAIAGHTYSWSPATGLSN
                     ALIAQPLASQAGNYTLTVTNTANGCIDTDIVVVTENKTPPIANAGADKVLTCAVTSQQ
                     IGTAAIAGNTYAWSPSTNLSNDAIAQPIASAVGNYILTVTNNTNGCTATDVVSITQNI
                     TPPLANAGANKILTCANPTQQIGTAAIAGNTYAWSPAAGLSSSSIAQPNASNIGTYTL
                     TVTNTANGCTATDLVTVTENRTAPSANAGIDKEITCAINSVLIGTPATAGNTYSWSPI
                     TGLSDPLIAQPTTNTNGNYTLTVTNTVNGCKATDVVSVTKNTTAPTANAGNNKVLTCT
                     NTTQQIGSPAIAGNTYAWSPAIGLSNDLIAQPIASQANTYTLTVTNTANGCTATDVVS
                     VTQNITPPIADAGANKILTCANPTQQIGTPTIAGNTYAWSPTFGLSSNSIAQPTANAI
                     GNYTLTVTNNVNGCTASDVVGVTQDISTPIANAGADKLLTCAVTSQQIGSTPIAGNTY
                     SWSPTLGLSDADIAQPLSTNPGNYTLTVTKNSNGCKATDVVTVTQNITPPTADAGANK
                     VLTCAITTQQIGSPAIAGNTYAWSPNIGLSNYGIAEPIANAIGNYTVTVTNTANGCKA
                     TDVVNVSQNISSPIANAGADKVLTCAITSQQIGTAAISGNTYAWSPSANLSNDAIAQP
                     TASAPGNYTVTVTNNTNGCSSTDVVAITQNTTPPTANAGSNVLLTCTNPLLQIGTAAI
                     AGNTYTWSPAIGLSSTSSAQTMVNKAGTYTVTVKDNSNGCTASDQLVINIDTIKPFAD
                     AGISKTLTCNQTAHTIGSSAIAGHTYSWSPGVGLNNANIAQPIASSMNTYTVTVTRLS
                     NGCIAKDSVFINQNITTSMAAAGDDLSITCTKQSHVIGTPATPNSTYSWSPAIGLNNP
                     NIAQPTVNKPGTYSLTVTNTITGCISNDVVNIPIDTLPPNANAGSDKTLNCLEKKVSI
                     GNAGLNGMSYTWSSNGVVFANTAQPQVSNTGTYVMTITKNASGCKNTDSMQVFIDTIK
                     PIADAGVDQTFDCPNIPLVLGVAPQSNTTYQWFGKRISDVLAAQPTTDTSGVFSLIVT
                     NATNHCKSKPDTVLIFPRNCDCLFFIPTAFSPNNDGYNDILQPLKYCDDYSDLIFSVF
                     NRWGELVFKSTSIDIGWNGEFKNESQLVDNYIWTLEYYDVLNQTNRFTKGVTTLLK"
     gene            complement(9456..10277)
                     /locus_tag="PLL59_02440"
     CDS             complement(9456..10277)
                     /locus_tag="PLL59_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22690.1"
                     /translation="MIKKFYTLLILITLGFCYQESKAQLPYIKPCYGINAGNDTAVCS
                     NVCTNATRVAIPQYDNRATNTYLVQQMNMPTPAYNTTGNNVIVDLDDIWSSIVNIGFN
                     FCFFGATYNKIVIGANGLVSFNTAYANAYCQWSYTGGIPDVAKPINSIMGPYMDIDPS
                     IGFNANRINYYTEGTAPCRRFVINFFQVPLFSSSCSTLKLTSQIILHEAYNIIDIAIT
                     NKPACTSWNSGNTIIGIQNAAGTSVFNAPLVTILAQHHLQINAGAFYQMAHCNNL"
     gene            10604..11332
                     /locus_tag="PLL59_02445"
     CDS             10604..11332
                     /locus_tag="PLL59_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMY22691.1"
                     /translation="MRKEAQYFDDIVQARRSIRIYDDKATFDETAVDRSLERALLSPN
                     SSNLQLWEFYRVKSKAKKNELAEYCLGQNAAKTANELIVFVTRKDKWKERARFVLAEA
                     KKEFPATLSKKHKLIIEYYDKLIPTLYFSDPVDIAGGFKKIASVVSGTFQPIPREVTA
                     TDMRIVVHKSVALAAQTFMLSMKAEGYDTCPMEGFDSVRVKKFLNLPRGAEICMIVSV
                     GTAKPEGVYGKRVRIPKEQVIFEI"
     gene            11332..11934
                     /locus_tag="PLL59_02450"
     CDS             11332..11934
                     /locus_tag="PLL59_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HMY22692.1"
                     /translation="MEPLIEGLIAGITVSFLIGPIFFALAEITMTKGWRCGLAYVFGV
                     IISDVVLIYAIEKVLNIFPFDDALKFKIGIIGGILLIVFGLSVFLTKANIKQYDVTNV
                     KTLLGAFAKGVTINVFNPFVTVWWITMYSTISINYIALNDKIVFYFAILLMVFLFDLL
                     KMRFAYYLKQKLTVQKLSIVKKGVGICLFIFGLAMIIKVS"
     gene            complement(12000..12899)
                     /locus_tag="PLL59_02455"
     CDS             complement(12000..12899)
                     /locus_tag="PLL59_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY22693.1"
                     /translation="MKILIIGASGLVGNNCLRYFKQKDWNCVGTYFSYEAANTVFFDT
                     LNLDNENNFNVTQFKPNVIVHCGALTHVDYCEQNEAESYEKTVVSTKNVITLANELQA
                     KLVFISTDYVFDGENGPYDEEAIVRPISVYGKHKLEAEQAVLAANAQNLVLRITNVYG
                     DEERGKNFVSRIIDQIFEQQKLTLRLPIDQYATPINAADIARCMYLLLRDNKSGIYNI
                     AGTDYMNRVQLALTILKYFPEAEYELIPQTTTQIAAPAARPLQGGLKNKKFMSEYPDF
                     RFNTVDDYVNHKSIMLDDMEDAE"
     gene            complement(13002..14039)
                     /locus_tag="PLL59_02460"
     CDS             complement(13002..14039)
                     /locus_tag="PLL59_02460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY22694.1"
                     /translation="MQKVSVVILNYNGKNWLEKFLPNVLENTTYPNAEIIVADNASTD
                     DSIPFLQASFPSVKIVQNSVNTGYAGGYNEALQHIKSNYYVLLNSDVEVTPNWIENII
                     SYMQLDTNIAACQPKILSYHEKNKFEYAGAAGGFIDKYGYPFCRGRIFDTCEIDAGQY
                     DNDGEIFWASGACLFIQADLFHKVGGFDADFFAHMEEIDLCWRLKNLGYKIAYCSQSK
                     VYHVGGGTLNKSNPRKTLLNFRNNRKLLRKNLTENELQSIYFTRNRLDQLAAVVALAK
                     GNIKEAQAIRQGLNEYKSSIEKWNKKRSENEKLQTTIAIGKPNKYGILKESIIWLYFV
                     KSIKKFNQIKW"
     gene            complement(14113..14994)
                     /locus_tag="PLL59_02465"
     CDS             complement(14113..14994)
                     /locus_tag="PLL59_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMY22695.1"
                     /translation="MQWLLTKIGIFYARLLSYFPLNLLFTLADIIYFIFNRIYAYRKT
                     IIDLNLMNAFPHKKSEELWFIRSNYYRHLADLIIETIKSISISKNELKARLQLQHDSY
                     ALLKNYENRGQHIFVVLGHYGNWEWASLLAGITSKLPSYALYSPPSNKTFEHFLLKNR
                     ARFGCQLVAANQLKTLYANLLKQTSLVAILADQTPVDTENAYWTKFMSINTPFYKGFD
                     TLARRTDAIVLYASIRKINRAYYELSFETITENASQTAENEIVEKYVRLLESDIQQRP
                     EYWLWSHRRWKRAGISY"
     gene            15136..15762
                     /locus_tag="PLL59_02470"
     CDS             15136..15762
                     /locus_tag="PLL59_02470"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HMY22696.1"
                     /translation="MSAILIESQYIGSCTYWHCLLQATSIVIDQEEHYVRRSFRNRAH
                     ILGANGLLRLSIPLERGKHQHSKMKDVKISYQENWQALHWHSITSCYRRSPYFEYYED
                     YFKPFYTEKQELLLDYNLSFMQTIAGLLKIKLPISKSDKYYTSADFIGIDARSYFLPT
                     KNITIDFPTYPQVFNDRFPFVKDLSLLDVLFNVGPNTSNYISSLPFKF"
     gene            complement(15752..16585)
                     /locus_tag="PLL59_02475"
     CDS             complement(15752..16585)
                     /locus_tag="PLL59_02475"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMY22697.1"
                     /translation="MKKTTSLLIGIFFLFVAATTPSATSKIGGIGGQAVFDSTAQRFK
                     VKSLEINHYINSKTLKPGDLILEIDKIPIHKMGYGDVLKLVQGNVGTPMSLKVLRYNA
                     IEKYYEINRIRVTLDMNPTWWSVPDYQYFNFLDAVNYSIGQLKVNGRNSIDSVKIPNA
                     KDRVYCNFNIKGAYESVYINNGKGRYYYNCSFVKTEDRSKAEGMYNYLVLQLRNYNIK
                     NVQLTKAENIVQDSKSYNLKPKEVADFSLVNLNIDVKLKKEFDSDEKKELWKVEMDIK
                     I"
     gene            complement(16686..18206)
                     /locus_tag="PLL59_02480"
     CDS             complement(16686..18206)
                     /locus_tag="PLL59_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY22698.1"
                     /translation="MSALSYYFNEDHETFRKSIKDFLEKEATPHIDNWEKDGKIPREF
                     WKKFGEMGYFGLAYPAAYGGSDLDFFYTVVMIEEISKIFSGGFAITAAVQTCMSMPYI
                     LHHGSDYLKETFLKPGIAGEKIGCIGITEPGAGSDVQNIQTKAIKEGDNYIVNGSKTF
                     ITNGIYGHFVILVCKTNPAAGAAGVSLLVVDLDSEGISKNKLNKLGWHASDTAELHFD
                     NVKVPTKNLLGEEGKGFYYLMGGLQLERLAGSIMGYSACEGMLQYGMEYMSQREAFGR
                     PINKFQVLRHRVAQLAAEIEATKFYVLQTCRMHNEKKYAVKESSIAKLLASELADKTA
                     YQVLQFFGGYGYMEDFKIARAFRDSRIGTIGGGTSEIMREIIAKMVIDDANYNRATSE
                     NSQQATLQIPTDIDGIFNTLPSRFKKEKANGKNINVLFEFENDLRYAVMIENGNISIR
                     KEKNLDTYDLIITTQTETYIAVETGKINPQEAFMSGKIQVSDLMKMMEFGALFKKL"
     gene            complement(18279..18629)
                     /locus_tag="PLL59_02485"
     CDS             complement(18279..18629)
                     /locus_tag="PLL59_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMY22699.1"
                     /translation="MEKKDNPILNLTFKFSVQIVLYCELLETRKKYVISKQLLKSGTS
                     IGANIREAQNAESKSDFIHKIKVALKEVEETEYWLEICKAVESYPDCDNLITNLHEIK
                     KILAKIISTSKKIT"
     gene            complement(18637..20160)
                     /locus_tag="PLL59_02490"
     CDS             complement(18637..20160)
                     /locus_tag="PLL59_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY22700.1"
                     /translation="MTALEQYYLTEEHQLFRKTLRDFLDKEAMPNIEKWEKDGFLPRE
                     IFKKFGEMGFFGITQEEKYGGANLDFWYDVIFIEETSKCWSGGFGASISAHPYLSMSH
                     LKHEASEQLKEKYLPKAIRGEIVGALAITEPHAGSDVAGIKTSAIKQGDKYIINGSKC
                     FITNGVLADYIIVACKTANTGSSGISMILVEGNSKGLTRNNLNKLGWKASDTAEIAFD
                     NVEVPIGNLLGEENKGFYYIMQRFELERLTLALGALSSSELMLQYTLQYMSERSAFGK
                     PINKFQVLRHKIADLFAEVESVKAYTYLVCQMHNDKKYCVKEASMSKLLATELSDKLA
                     YQCLQMFGGYGYMEEYKAAQFFRDSRLGTIGGGSSEIMREIIAKMVIDDIDYEKREVR
                     NEIKNITIDELFATLPARFKKEKVNENEMNVLFEFEENLHYSILINKEDLSIQKGKNQ
                     SNFDLIITTNTATYIEVETGKLNPQEAFMQGKIQVSDLSKMMQFGTMFRKFNNESMK"
     gene            complement(20166..21029)
                     /locus_tag="PLL59_02495"
     CDS             complement(20166..21029)
                     /locus_tag="PLL59_02495"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HMY22701.1"
                     /translation="MSNKKLILSAALTGAATNRAHCPAIPYTPKELGEEAKRAVDAGA
                     SIVHIHAREDNGIPSWRTEVFEEIKAEVRKHSADVIINFSTGAIGLSVEERIKHLPVT
                     KPDMAAFNMGSMNYAIFSKKQKQFYWNAVFENSFDTMIKVVKTMNENNICPEMECFDT
                     GHIYNVEPLREMGLIPDNACYSLVMGVLGGIPATTENLIHQIKQVPEGAFWQVIGISR
                     KQWQLAATACTMGGNFRVGLEDNFYLPNGEMAKSNGECVEWGAKLARMLDREIASISE
                     TREMLKLQKIN"
     gene            complement(21062..21886)
                     /locus_tag="PLL59_02500"
     CDS             complement(21062..21886)
                     /locus_tag="PLL59_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMY22702.1"
                     /translation="MIYTQEQTSNFTKQNFAYIIVEEKDNVLTITLNRPEKRNAFHAP
                     LANELAYALAYAHYNNAIWCVVLKANGPTFCAGADLKAFAGDDTTEKQSTIPMPKDMV
                     KLGDEFNGLHKPCIAQVHADVYAGGFLMIGGCTHVIAVESAKFGLPEVKRGIFPFQVM
                     ATLEPLMSARQLLDLCIRAKILNAKEAQEIGIVSEVVKEENLASRVQELIAEIKEQSP
                     TAIRLGLKAFQEMKTKNADEKHKYLHAMLMQCLQSKDAAEGITAFKEKRKPEWKGE"
     gene            complement(22092..22439)
                     /locus_tag="PLL59_02505"
     CDS             complement(22092..22439)
                     /locus_tag="PLL59_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMY22703.1"
                     /translation="MKINKKEPCIYIISNTFGNVIYIGVTSDLWNRILQHKQGDGSVF
                     TKKYKINKLIYFEEFTNMMEAIEREKQLKKWNRKWKDELIDTMNIERIDLAKDWYSEK
                     DIIEYNQSRKYFI"
     gene            complement(22661..24694)
                     /locus_tag="PLL59_02510"
     CDS             complement(22661..24694)
                     /locus_tag="PLL59_02510"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMY22704.1"
                     /translation="MSQYSILNKQYSINKLLVANRGEIAIRIFRTCKEMGISTVAVFS
                     DADANSLFVKMADESVRIGGKQPNESYLVIDKIIEAAKKTGANAIHPGYGFLSEQVEF
                     AQRVISEGIIWVGPHIKAIEVMGSKIGAKKIMQQHNVPTIPGYQGDDQSDATIKTKAI
                     EIGFPVLLKASAGGGGKGMRIVREEKELDKAIAEAKSEALNGFGDDTLLIEKYFDTSR
                     HIEFQIFGDKHGNALHLFERECSIQRRYQKVVEESPSPFISDETRQKMSSAAIAACKA
                     INYDNAGTVEFIVAPDQSFYFLEVNTRLQVEHPVTEEVTGLDLVRLQIEVAQGNPIPF
                     QQEVIKQNGHAIEVRLYAEDPSNNFLPVNGKILDWIPAINDGLRYDTGIESGAEISTY
                     YDPMIAKIIAKGKNRIETIQKLKSALQETVCSGFTTNKNFLISILENQDFQNGNFDTH
                     FLEKQFNYKGEQYAQETLDILTCALIHFRYLEREENRNIAQGVPSGWRNNFYQALQEK
                     YSFQGFEFPISYRNINNELHISFAEKDFISTHRQSTKGIQQSSNAWCPQQPYTFEINN
                     IRRNFYISQNGHQYFVQSPQLGQIVLHSIDRFPAPQTEIMKGAYIAPMPGDITTVLVK
                     QGNQVKSGDALLKMISMKMESTIEAHSDGEVEEIYVTEKQFVESGTLLLKMKE"
     gene            complement(24735..25094)
                     /locus_tag="PLL59_02515"
     CDS             complement(24735..25094)
                     /locus_tag="PLL59_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMY22705.1"
                     /translation="MNSTELKNRTKKFAVNTIKFTESIKPTYSNVIIIKQVIRAATSV
                     GANYRSSLRGKSNPDFINKINIVQEEADECIYWFELLGETNYKLKDLDILLKEANELT
                     AIFSAILKTLKLKSVKK"
     gene            complement(25144..26751)
                     /locus_tag="PLL59_02520"
     CDS             complement(25144..26751)
                     /locus_tag="PLL59_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMY22706.1"
                     /translation="MSILQTKINTNSAAYKDNYQKMLSKIEEMNKHLKQSLFQGEEKH
                     ISKAKSQGKLLARERIEMLLDQDSPFLELCPLAGLGVKGGFGTGGTMVAGIGFVSNKM
                     CMITANVGTNKGGAIDIATLKKAIRISEIASENNLPCINLVESAGANLPDQAQVFNLG
                     GNNFKNITRHSKKGLPTISIVFGNSTAGGAYIPGMSDYTIFIKDKAKVFLAGPPLVKM
                     ATNEIVDDETLGGAEMHSKISGVSDYLAQDELDGIRIARELMSNLNLKSEIINLKSEI
                     SYKEPNYPIDELLGIVSVDVKIPFDCREVIARIVDGSEFHEFKKEYGTTLVCGFAKIH
                     GYSIAIIGNNGVLFNDTANKGAHFIQLCNMNHTPILFLQNITGFMVGKQYEQNGIIKD
                     GAKMINAVSNSEVPMFTLMMGASYGAGNYAMCGRAYNPRFLFSYPNSMIAVMGAEQLA
                     GVMQMVSKEAAIKSGQQYDEAQATQMKEFMKAEIEKQSNAFFASGQLWDDGIIDPRDT
                     RNVLGLVLALTYMNEVKGTSSYGVYRM"
     gene            27003..27329
                     /locus_tag="PLL59_02525"
     CDS             27003..27329
                     /locus_tag="PLL59_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HMY22707.1"
                     /translation="MTAKEIMLGLPARLKTEEAAGQSGTFHFQLEGETGGDFTVSVAD
                     GACTVTEGLSGEADCVISAAANDFEDAEEGRVNKQMAVMMGKIKISNLGAMLKFMGLF
                     SDLEAA"
     gene            27480..28799
                     /locus_tag="PLL59_02530"
     CDS             27480..28799
                     /locus_tag="PLL59_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22708.1"
                     /translation="MKTRTKINVLVLFIALITSLFTVSCKKEKALDSKDYSNIKIANW
                     RDYIGDEFGIEGYIIDEGNGHPQIISDDENYLFDGLFPESEYLHLDMASRNDDLRRFH
                     GRKVKIKGILEANTDQSIVGTASFLGDPSLATLRVSSINAIPIDSVSYFRPTTNWNFC
                     ERYPNVCSTFVTPIGIKCAILYSGGINASKAYTRYWNDIKFMYNILINNGYLAANIRV
                     VYRDGIGNDGSIPVHYAATPTGFNDAVEYMKTKLNSQSKLFLMFNNHGGGYETSTGNN
                     YGVLDANNDEAEASNKTDENYCYYGVSAPFLDDTIAAKINRLPFYELIAVVKPCFSGG
                     LIWDLRGANRTIITSGTEFQVTYPNPGGQFGELSYNFFGAVTGMTPDGVVVDADINND
                     GKVSMYEAYIFCRDNERRNEQPQFGNSASGTPTTTPSSSGYGSNVFL"
     gene            28896..29594
                     /locus_tag="PLL59_02535"
     CDS             28896..29594
                     /locus_tag="PLL59_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22709.1"
                     /translation="MESFQIKYSEGRNLAIIIITLMLVIAALVGVMLFAFLKMDIDGD
                     IGAIIFGVIITLCSILLGVALIVMFKVKGVVSFSENLIEINLNKSTLLYKNTNIKIPF
                     SNIKNAVLENDTYNRKVISLSIVAPSKTIYLAPISNDENDNFDQFWNNLDALIQKYNQ
                     SQKNIGQQIQHKSMYEQWWAKLLAIICAVLAIGICIAKIVDQDAISTWRLIAFLAYTI
                     PFCFIVYQANKKKK"
     gene            29597..30463
                     /locus_tag="PLL59_02540"
     CDS             29597..30463
                     /locus_tag="PLL59_02540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY22710.1"
                     /translation="MKDKIVVITGSNSGIGRVAATEIAKMGAQVIMVCRNEAKAKAVQ
                     EDINAVTGLNNCDLFICDFSSHASIKQFAKAIRMKYNQIDVLINNAGVIIGEREENEE
                     GMEMMFATNHLGYFLVTHYLLDLLKASDKARIVSVASLAHRFTNMKWNNLQGEQSFNS
                     WIQYGHTKLMNILFNKELAYQLKKNTNITANCLHPGNINSNFGQSGNPLMKFLMSNFG
                     FVLTTPEKGAETTIYLATSPEVQGKTGLYWYSKNPTFPSLEAISSENSRRLWEISHKL
                     SGVKEFGVVNLD"
     gene            30480..31217
                     /locus_tag="PLL59_02545"
     CDS             30480..31217
                     /locus_tag="PLL59_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22711.1"
                     /translation="MDIFSFFTTNFPPLSLIARLFPISILWSFAALTFAGYCKKKFHF
                     KTGYTRKIFHFLIFISAVFFQIFYSLQGVFILGWSVTVVLIFACYKGNGFMLYEAIAR
                     EKDAPYRTKYIVYSYLATFLGGVFSNILFGQYAIFGYAITGVADAIAEPIGTRFGKHQ
                     YRVFSFDKMKVSYRSIEGSLSVLVATLLVIVLLATTFFSVSIPFYYICSIAILCMLVE
                     AVSPSGFDNLLLQLCSSYACYFIIQLL"
     gene            complement(31214..32650)
                     /locus_tag="PLL59_02550"
     CDS             complement(31214..32650)
                     /locus_tag="PLL59_02550"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMY22712.1"
                     /translation="MKPLFTLLFITLFTTITFAQENNFNITITIKGMENQDGLLANYY
                     GDKKYLKDTLHFNEKGVAQIKGNKNIPAGVYLMAFPSMRYASFDFIIKETAFSIQTDT
                     SNFIKHAVIKNSVENKQLFEDMNYMIPLGVQNDSLQKLLKPLEKNSDAYKKIADQLDN
                     ISEDINSHRKKIAKKYPTTFYSKLIKAMLELEIPEAPKNAKGELIDSFYTFHYTKQHY
                     FDHIDFADSGFIRSPIFQGKLLKYFDVYTFPQPDSIIQSIDFVLDKAQANAEMFQFCL
                     NEIFLKYAKSEIMGHDAIYVYMSDKYFFTGKAWWASEKSLSELRDRVEAIRPTLIGKI
                     APNFIVQDTSGKNQIFHDFISKNKYTVLVFWNSDCGHCQKEIPLLKQIYTDSLKQFGL
                     DVFSVSTEQTDSSFRAFAAKNCSTDWITCADMRGVSAFRKEYDVIATPKVFIIDRNYK
                     IVAKNIPVEKLYDFITFIDKNNAKRKDD"
     gene            32744..33121
                     /gene="crcB"
                     /locus_tag="PLL59_02555"
     CDS             32744..33121
                     /gene="crcB"
                     /locus_tag="PLL59_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HMY22713.1"
                     /translation="MLKYLYIAGGGALGTICRYWLVTCLEQKYTSTFPFGTLTVNLFG
                     SLMIGFLFGLMSANGIIEEKWRLLLFIGFLGGFTTFSSFSLENMKLLQNGFSSTAIVY
                     IIASNLFGILLAFLGYFISLKFK"
     gene            complement(33170..33979)
                     /locus_tag="PLL59_02560"
     CDS             complement(33170..33979)
                     /locus_tag="PLL59_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMY22714.1"
                     /translation="MKKLTSLIVFATLFLANNSFAQNVKEMIPKVMAAMGNAKTMTYH
                     FYAQERMHDGKYSKSDVEFTVIASPLKIYANAHKPQSAQLFYDAAVSTDVKVKKGFKL
                     NLSPTNSLLMKGVHNPVTRAGFGQIKKILETSMAQRKGEDLTTYVKVLGTVTYDNQEC
                     WKIEINDADYKIIDYTVAAGEKSVWQIGKKLAISEYKIKELNDIDDDVKPGQKLKIPS
                     SYAKKTTLYIDKSNLLPIYHKMEDEKGIYEIYEFKDLKLGVNLTDANFQFK"
     gene            complement(34035..35381)
                     /gene="argH"
                     /locus_tag="PLL59_02565"
     CDS             complement(34035..35381)
                     /gene="argH"
                     /locus_tag="PLL59_02565"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HMY22715.1"
                     /translation="MAKLWQKGNLDENELAKQVETFTVGDDRNLDTLLAKADVLGSLA
                     HTKMLAAVGLLPQENLAPIHQELKCIYAIIEKGNFSIHKDSEDIHSDIEFRLTEKLGN
                     LGKQIHSGRSRNDQVLLDLKIFTRFEIERIANATQQLFDTLLKLSIQHKDKLLPGYTH
                     LQLAMPSSFGLWFGAYAESLVDDMETMYAAFTLANKNPLGSGAGYGGSFPLNRKMTTE
                     LLGFADLNYNVVYAQMNRGKMEKTVVAAIANIAATLAKLAMDACLYLNQNFGFISFPD
                     VLTTGSSIMPHKKNPDVFELIRAKCNKLQSLPNQFNLILSNLPSGYHRDLQIIKEDYM
                     HSFSTIISCLEMSNIMLSHIEIKDNILEDEKYKLLFTVETVNQLVNEGMPFRDAYITV
                     GKQVETGNYKKPSVIHHTHEGSIGNLCHEEIEAMMQNTMAQFPFQKINSTIEKLLA"
     gene            complement(35441..36205)
                     /locus_tag="PLL59_02570"
     CDS             complement(35441..36205)
                     /locus_tag="PLL59_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HMY22716.1"
                     /translation="MEEIKLSKIATVAPKSLDKELTKVKTKELILQIAELQDVLIASQ
                     KRSLLVILQGMDASGKDGAIKKVFSGINPAGLRVQTFKRPTEEELAHDFLWRVHQVAP
                     QKGEITIFNRSHYEDVLIQRVHNWIDENTVKQRFDAINNFEKLLEQNGTKIIKCYMHI
                     SPEAQLERLEERKVNPAKMWKHRDNDWKERELWKQYRNAYEDAFRNCSKAAKWNIIAV
                     DQNWYKEYQIALLVRDALKAMKLEYPALETAVAPKK"
     gene            complement(36230..37792)
                     /locus_tag="PLL59_02575"
     CDS             complement(36230..37792)
                     /locus_tag="PLL59_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HMY22717.1"
                     /translation="MASQEEFIQTIQQGYQFKGTSITVGGAMLDAKSIANTLINIPLK
                     TLNRHGLIAGATGTGKTKTIQTISEKLSENGVSVLMMDIKGDFSGIAEPGVSNQKITD
                     RVVAIGNTWNPKAYPVELMSISSQDGVRLRATVTEFGPVLISKILDLNDTQQGVVAMV
                     FKFCDDQGFGLVDLPDFKKVLQFLTNEGKDIFEKEYGAISPATVGTILRKVVELEQQG
                     AAMFFGETSFNVNDLLRKDENGYGYVNIVRLTDIQDKPKLFSTFMLSLLAEVYAKFPE
                     RGDMDQPELVIFMDEAHLIFDNASKALQDQLEAIIKLIRSKGVGIFFCTQNPNDIPES
                     ILAQLGLKVQHALRAFTAKDRQAIKLVAQNYPETSYYKVEDLITQLGIGEAFVTALNE
                     KGIPTPLVHALMTPPQSRMDILNEEEMKTLIQKSNLYKKYKDTIDPESAYEILNKKIE
                     QAQQIEQQQEVAEQQAKEAEKQAKIDAKVAKTQKSTLDKVLNSTVTKQIARTATTTIT
                     RGLLGALKGMFK"
     gene            complement(37923..>39316)
                     /locus_tag="PLL59_02580"
     CDS             complement(37923..>39316)
                     /locus_tag="PLL59_02580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMY22718.1"
                     /translation="DMKLKICGAAQTVTGSCHLLTLEDGFTILLDCGLYQGHEDEFED
                     FNSKWYFNPSAIDVLILSHAHIDHCGRIPKLVKDGFRGKIYCTSATRDLAAIMLLDSA
                     HIQESDAAYASKKNRKPQKPLYTQLDAKNCLKYFRTVEYDIWFSIKNNVKVFFADAGH
                     ILGSASVTLNIKEKNEEITFGFSGDIGRYNRPILKDPSPMPALDYLICESTYGGKKHE
                     ETPESDEHFLQIIVETCIEKRGKLIIPAFSVGRTQELLYRLDKLYTAGKLENIPVFVD
                     SPLAMNATEIFVVHPECFDEEIHQYMRTDENPFGWNNMHYTRDAEHSKRLNASDEPCI
                     IISASGMANAGRIKHHLLHQIDKAENTILIVGYCAVGTLGQQLTQKPERIKIFGEEKK
                     VKAQIEIMSSMSAHADEPELLQFLANQDKKKLKKIFLVHGEPKRQEAFKTTLLQENYK
                     SIEIPELGAHFEL"
BASE COUNT        11715 a         6691 c         6579 g        14331 t
ORIGIN      
        1 gtaatattct gccgaattcc tcataagttt taagcttact taagttagga tcattttcag
       61 ctttgtcgcg attattaaat cctgatttta cagcttcttt taatgctttc aatgcttgaa
      121 acatttcatt agtttgcgta agcgaacaag cacgattgta cataatttct gcatatcttg
      181 ctgcattggc taatttgtct ttgtcagaca aactttgata cgtgctcaac caaacgtcaa
      241 tggttttatt agcattgccg tattgttttg cggcaatgta tttttctgac atttgttgca
      301 tttgcgcaga gtacgtttca aatgttgatg cgtttaatgt tggttttgcg attgcgatag
      361 aacaggcgat gcccatggct atcaaaagtg ataacttttt catgtgattt tagaattaat
      421 tgttaaaaat taaagtttat ttagttttat tttattggtg ttaaagacaa acaatatctt
      481 acaatattgt ctgccattaa catattttaa taattaatcg atggcgacga gttcaccgtt
      541 gaccatttta aaaatataat gttctctgct aagtctgttt tcaaatattt cttgcataga
      601 atcatctaat ctgatatttg catcttccaa acgctcacgt gcagcatcta atttttcttg
      661 tgctttatca agtgcttcag tgatgtcgcc attatcatct tctatgttaa ttataatttt
      721 agatgatgaa ctagtatcgc cgtttttaat cactacttta tggctgcttt tagttgctgt
      781 tggcggcaat ggtggcaaag gaggtactgg tggaattata gttgagccat tgcgaatagg
      841 ttcccagttg atagcaattt ttcccatgtt catatatcct tctgtgcgct cgtcccaacg
      901 aattactttt ccttcaggta catacacttt gattttcaat ttttgatttc tgtatttgat
      961 tttattattg tcaaattgaa tgtatggatc gattaataat ttattgttga cttgtctgat
     1021 gttgtgctta aatgatgttg catttattac tgcatctttc atgtcttttc cgtttgctgc
     1081 atattctttt acaacaaaaa atgaatcatt tctgcttgga tatacttcta tttcaacagg
     1141 aaataaattg tcttcatgat cagaaacaaa ctctaataag tcactgacac cattgatatt
     1201 gatattgtca tagtcttcat cttgaatttc aggattcatt gcaatttgta atgtgtctga
     1261 ttgataagca tagttcagtg gatagctttg aataattttc tttttttctt gtactaattt
     1321 cttagcatta tagccactga tattgagcaa taagatacca aacaagaaca atcctaaaag
     1381 cggaaataat gtttttttgt ttaatggttt taaggtttta gataaaacat gaacgatagc
     1441 aagaatactg aaaataatag gaatgataca agcgagcaat gcaccaatac cgaaaaacca
     1501 accgtcagaa gtatttgtaa agaaatagct attggcaaca ggaataccga ataatgctaa
     1561 gcaggaaatc acaaccaaag tgatgagcaa actaatgatg acaatgcttc caattaaaat
     1621 tgcacaagca atgccaatcc ataataatgc tttaaaaaac aacattatac ctgcaccaaa
     1681 gaaggcgata atgcgattaa aaaagccttt tgatttcaca tctatttgtt cttcgtttat
     1741 gttttttaca atgttggata aattgattgg ctcgcctttc atttccaatt tttgcgctgc
     1801 tgtttccgct tttggcataa tgagactcaa tactatatac actattattg gagaaccaaa
     1861 acctatgatg gttaaaccga taaaaagtaa acgtaaccaa atcgcatcgt taataccaaa
     1921 gtaagcactt aaaccagcac aaacacctgt aatcaaacca ttgtcgaagt cgcgatacaa
     1981 ttttttaccg ccaacttgat ttgtaaattc tgttattgta gtgctattat tattacttgt
     2041 gttattgcta ttgttttgcg aattttcttc atcaaattcc tgtggatttc ccattaagtt
     2101 gattactgtt gtcgcatctt ctttagtaac aatatagttt ttgccttttg ctaaaaataa
     2161 ttcagcaaaa cgagcttcta tatctcggat tatttcatct ttatcagatt cgtttgcata
     2221 aaatgcacga agtgaattga aataagcgga gagttgttcg tacgcatctt cattaatatt
     2281 gaagatttgt ccagccaggt ttatattgaa tgtcttaatc atgacgtatt tttttaagtt
     2341 tagttgattt ttgatttttt aaatggaaat attgtctgct tctggtttag aataatgagt
     2401 ggctttatcg gctgcttcgg aaagttcatt ccaagtttgt tttaactctt ccaaagctgt
     2461 ttgaccttct agagtaagtg taaagtattt tctaggcgga ccagatgtag attctttcca
     2521 ttcataagtg agcaatcctg catttttaag gcgtgttaat aatggataaa cggtaccttc
     2581 cactacgagc atattggcgt tttttaaagc atctaaaata tcagatgcat acacctcacc
     2641 acgtgaaatg ataccgagaa tacaaaactc caatatgcct tttcgcatct gtactttagt
     2701 attttcgata ctaaacgctg agcttgatgt tgttgtcata tttgtaatta tttttaaatg
     2761 ttagtgatta tgttgctgcg ttgcattgtt cttattttgt tatcatcaac ttcattgagt
     2821 acaactttcc aggatttata ttttgactat atgttgcact ttcaataaat ttgactacat
     2881 tttcttttgc ctcttggttg tcgctattga tgtaagtaat catagctttt ccattttcct
     2941 cgatgatgta gtcaacatca actacgctag gtgtgtagtt tttttcaagg tctgtgtttt
     3001 tatcaaacgc agaagcaaaa tccatttttt ctaccactat ttctttttgt gctgcttttt
     3061 cgctttttat acttttagca gttacgttgt tttcgagtgc aggtaacatt acaaatgtca
     3121 aggctgcgat aattaaattg ttcataattt tattttttaa ttgttatact tatttatttt
     3181 accaagtaat tgatgataca aagataagta caaaacaatg tattgtgcaa tacatagtac
     3241 taatatttaa cattaataaa tctaaataat tgataatcaa tactaaaaat tttatttaat
     3301 ttttgttatt tatgctaaat tgtaataaaa ttgtattttt ttagcgtatt tttgaattat
     3361 gagacgagtc aatcttagca ttatttttat ttttttcttg caatttttag caattgacgt
     3421 attttctact gatggagaag tcaatcattc accatatgat gtggtgtata atcatattca
     3481 tttcttgaaa aaaggtagtt atgatgagct ccaatctgct gcttcttttg acatacctaa
     3541 taaaaaaaat cgcatagatg cagcaattac tttgaaagaa atattagatg ctaaaattga
     3601 tccatctgca ttattgaata aaataccaga tgatccaagc tttacagatt ctaccagcaa
     3661 acgccatatt tatgtgcttt acgataaatt gccgcaggtt tatgtcgagc tgaaaggcaa
     3721 taaatggtat tattcacaag ctactgttga tgcattacca caattacatc gtaaaatatt
     3781 tccatttggt actaatattt gggcaaagtg gtttcctatt aaaaacaata atgaatttct
     3841 gagattatat acctggcaat ggattggaat cggcattatt ttattagctt ttatactgtg
     3901 ttttacactg cttaaatatc tatttagatt tatttttcac aagattttat ttaagaaata
     3961 catcaatgaa attaatgata tagataaact gcgcactagt gccaacttgt tttcagtctg
     4021 gattggattt aaacttttcc aattatttat tccgactcta tttatcaatc caaaatatgc
     4081 aatgcctttg attacgggtg tcaattttgt agcagcatca atcattgttt tagtggtgta
     4141 taaattggta gaactaatta ttttctattc caaaaattat gtgcaaaaca cggcaacaca
     4201 atgggacgac caaatcgtaa tggtgcttca aaaattccta aaatttatcg tcattttttt
     4261 aggtttattt tatgtgctaa ataccttaga tgtaaacatt gcgaccatta ttgctggttt
     4321 atccgtcggc ggtttagcct tggcattagc agcacaagat actgtaaaaa acttcattgc
     4381 ttccgtgatg atttttatcg acaagccatt taaaataggc gatacaatta aaggagataa
     4441 ttttgaaggt gctgtgcagg aggttggttt tcgttctaca cgtatcaaaa cggcagaaga
     4501 ctctttggtg tatattgcta atgccaaatt atccgagatg attatcgaca acaaagggtt
     4561 tcgtgtcttc aaaaaatata aaacagaatt agccattgct catgccacac ctttatataa
     4621 gattgaacaa tttgttgctg gtattcgaac gatattattg aaatatcctt atactaaaaa
     4681 ttcaacgatt gatgtgtttt tgtcaaatat tcaaagtgca ggtttaagca ttacactaag
     4741 ctatacttat aaagtgtata gccaacgcga agaattacaa cacagagaat ttattctgtt
     4801 aaaaataatt gcattggctg aattattaca aataaaactt tttgaaacgt caccagtggt
     4861 gttagcaaat atagacaata ataaagctga tgaaaatatt gaatctgtga aacaaaaatt
     4921 agatcagttt tttacagatt tagatacaca aatttctaaa acaaaataat tgttgcttga
     4981 atttacaatt gtctatttca ataatgttgt aactcctttc gtaaatcgat ttgtttgatt
     5041 aagtacgtcg taatattcta atgtccagat gtaattatca actaattgtg attcgttttt
     5101 gaattcgcca ttccaaccta tatcaatgct ggtcgattta aagactaatt cgccccatct
     5161 attaaatact gaaaagatta agtcactata atcatcacaa tattttagtg gttgcaatat
     5221 atcattatag ccatcattgt tgggtgaaaa tgcagttggt ataaaaaata aacaatcgca
     5281 atttcgtgga aaaataagta ccgtatcagg tttggattta caatgatttg tagcatttgt
     5341 aacgattaat gaaaacacgc ctgatgtatc agttgttggc tgtgctgcaa gtacatcact
     5401 tattcttttt ccaaaccatt gatatgtagt gtttgattgt ggtgcaactc ctaacaccaa
     5461 tggtatgttt ggacaatcaa atgtttgatc cactccggcg tcagcaatag gttttatggt
     5521 atcaataaat acttgcatac tatctgtgtt tttgcaacca cttgcatttt ttgtaatagt
     5581 cattacgtat gttccagtgt tacttacttg tggctgcgct gtattcgcaa atactactcc
     5641 attacttgac cacgtataac tcatgccatt taatcctgca tttccaatag atactttttt
     5701 ctctaagcaa tttagcgttt tatcgcttcc agcattggca ttgggtggta aggtgtctat
     5761 cggaatatta acaacatcat tagaaataca accagtaatt gtattggtta cagttaatga
     5821 ataggtgcct ggtttattta ctgttggctg tgcaatattt ggattgttca atcctattgc
     5881 tggcgaccat gagtaagtac tgtttggtgt tgcaggtgtg cctatgacat ggctttgctt
     5941 ggtacaagta atactcaaat cgtcaccagc agcagccatt gatgttgtga tattttgatt
     6001 gatgaaaaca ctgtcttttg ctatacaacc atttgacaat cgtgtaacag ttacagtata
     6061 tgtattcata ctgctggcta ttggctgcgc tatgtttgca ttgtttaatc caactcctgg
     6121 agaccaagaa taggtatgac ctgcaatggc gcttgaaccg attgtgtgtg ccgtttgatt
     6181 acaagttaat gtttttgaaa ttcctgcgtc agcaaatggt tttatggtat caatattaat
     6241 aacaagctga tcacttgctg tgcaaccatt actattgtct ttaacagtga cagtatatgt
     6301 gcccgcttta ttaaccattg tctgtgctga tgatgttgaa gatagaccga ttgctggcga
     6361 ccatgtataa gtatttccag caatcgctgc agttccgatt tgtagcaatg gattagtaca
     6421 tgttaataaa acattgcttc ctgcattggc tgttggcgga gtcgtatttt gtgtgatggc
     6481 aacaacatct gttgaagaac aaccattggt attattagta acagttactg tgtaatttcc
     6541 aggtgcgctt gcagttggtt gagcgatggc gtcatttgat aaatttgcag aaggtgacca
     6601 agcgtaggta ttaccagaaa ttgccgcagt gcctatttgt tgtgatgtga tagcacaagt
     6661 taatacttta tctgcacctg cattagcaat tggtgacgag atattttgcg atacattgac
     6721 tacatcagtt gctttgcaac cattagctgt attagttaca gttacggtgt aatttcctat
     6781 ggcatttgca attggttcag caattccata attagacaat ccaatgtttg gcgaccatgc
     6841 ataagtattt ccagcaatcg ctggcgaacc aatttgttgc gtcgtaatag cacatgtcaa
     6901 tactttattt gcgccagcat cagcagttgg cggtgtgatg ttttgtgtaa cagtaaccac
     6961 atctgttgcc ttacaaccat tagaattttt tgttacagta agcgtgtaat ttccaggatt
     7021 agtgcttaat ggttgtgcaa tatcagcatc tgataaacca agtgttggcg accaagaata
     7081 agtattgcca gcaataggtg tggagccaat ttgttgagaa gtaacagcac aagtcaatag
     7141 tttatctgca ccagcattgg caattggtgt cgaaatatct tgtgttacgc caacgacatc
     7201 acttgctgtg caaccattta cattattggt cactgttagc gtatagtttc caatagcatt
     7261 tgctgtaggt tgtgcaattg aattagatga taaaccaaaa gttggcgacc atgcatatgt
     7321 gttgcctgca attgttggcg ttccaatttg ctgtgtcggg tttgcacatg ttaatatttt
     7381 atttgcacca gcatctgcaa ttggtggcgt tatattttgt gtaacagaaa ctacatctgt
     7441 tgcggtacaa ccatttgccg tattggtgac agtaagagta taagtattgg cttggctggc
     7501 aatgggctgt gctattaaat catttgacag accgatagca ggtgaccaag cataagtatt
     7561 tccagctata gcaggagaac caatttgttg tgtcgtattg gtacaagtca atactttatt
     7621 atttccagca tttgcagtgg gtgcagtcgt attttttgta acactgacca catctgtagc
     7681 tttgcaacca ttaacagtat tagttaccgt aagtgtataa tttccatttg tgttggtagt
     7741 tggttgtgca attaatggat cagataatcc agtaataggt gaccatgaat aggtatttcc
     7801 agcagtagct ggtgttccga taagtacgct atttattgca caggtaattt ctttatcaat
     7861 accagcatta gcacttggtg ctgtccgatt ttctgttaca gttacaaggt cagtcgcagt
     7921 acaaccattg gcagtgtttg ttacagtaag tgtataagtg ccgatattac ttgcattagg
     7981 ttgagctatg gaagagctag ataatccagc agctggcgac caagcataag tgtttccagc
     8041 aattgctgct gttccgattt gttgtgtagg atttgcgcaa gtcaatattt tatttgcacc
     8101 tgcattggct aatggcggtg ttatgttttg tgtaattgat acaacatcag tagcagtaca
     8161 accattagta ttgttggtaa cagtaagtat ataatttcct actgcacttg caatcggctg
     8221 cgcaatggcg tcatttgata aatttgtaga aggcgaccac gcgtaagtat taccagcaat
     8281 tgcagcagtg ccgatttgtt gtgatgtaac agcacaagtt aatactttat ctgcgcctgc
     8341 attagcaatt ggtggtgttt tattttctgt tacaacaaca atatctgtat caatacaacc
     8401 attagctgta ttggtgactg taagtgtgta gttgccagcc tgactggcta atggttgtgc
     8461 tataagcgca ttggataaac cagttgcagg cgaccacgag taggtgtgac cagcaattgc
     8521 aggagttcca atttgttgtg ttgttgccgt acaagttaat attttatcag cacctgcatt
     8581 tgctgttggt gctactatat cacgcgtaac cagcacagcg tcggtcgtaa agcaaccatt
     8641 tgaagttttg gtcactgtca aagtatatgt atttggcaag ctagcaattg gctgtgcaat
     8701 taaaggatca gataagcctg ttgtaggtga ccatgaatag gtatttcctg caactgcagc
     8761 ggttccaatt tgtcgtgttg gattagtaca agtgatttcc ttatcaggtc cagcatctgc
     8821 cgttggtaat aagatactgc cagttactat aacagcatct gttgttatac aaccattagc
     8881 gatattggtt actgttaatg tataagtatt tgctagtgta acattgggtt gtgcaatatt
     8941 tgggtcattt aaaccagttg aaggactcca agaatagcta ttcccagcaa caccagctgt
     9001 gcctattgta gttccgctac aagacagcac tttatctggg ccagcatttg caactggtgg
     9061 tgtattattg ctcgtcacag taacagcatc tgtagctgta cagccagttg ctgtattgcg
     9121 gacagttagc gtataggttc catttgcagt ggcaagtggt tgtgcaatgc cagcattatt
     9181 taaattgcta ttaggcgacc aagtataggt atgaccagca acagcaggcg taccaatctg
     9241 cacggaaggg cttgcacaac cgattacttt atctgggcca gcatctgctg tcggtttttg
     9301 attgacagca aaattgaaat tatctgatac aacaacggtg ctgccatcac atctgtcatt
     9361 gactagattg gcaataaaat tattgttgga agtcgggcaa acactttcat ttaatgttgc
     9421 tgtatttgac acgacatttc cgtttagtgt ccaatttata gattgttgca atgcgccatt
     9481 tggtaaaaag cgccagcatt tatttgtaaa tgatgctgtg ccaggattgt aaccagaggc
     9541 gcgttaaata ctgatgtccc tgctgcattt tgtatgccta taatcgtatt tccactattc
     9601 cacgatgtac atgctggttt attagtaata gcaatatcaa taatgttata tgcttcatgt
     9661 agaatgattt gtgatgttaa ttttaatgta ctacatgaag aactaaataa aggaacttga
     9721 aagaaattga ttacaaacct tctacaagga gctgttcctt ctgtgtaata attaatacga
     9781 tttgcattaa aaccgatact tggatcgatg tccatataag gccccataat ggagttaata
     9841 ggtttggcaa catctggaat tccacctgta tatgaccatt ggcaatacgc atttgcatat
     9901 gctgtattga aagatactaa tccattcgca ccgataacga ttttattata agttgcacca
     9961 aagaaacaaa aattgaatcc gatgtttaca atagaactcc aaatatcatc taagtcaaca
    10021 ataacattat ttccagttgt attataagct ggcgttggca tattcatttg ttggacgagg
    10081 taggtattcg tcgctctatt gtcatactgc ggtatggcta cacgtgttgc attagtgcat
    10141 acgttactac atacagcggt atcattacca gcattaatgc cataacatgg cttgatgtaa
    10201 ggtagttgcg ctttagattc ttggtaacaa aagccaagtg taattaaaat aagtaaagtg
    10261 taaaattttt taatcatcat tcaataactt aataaatgaa atgtaatttc caatcagatg
    10321 cgtaataaat tataaacaca tttatgcgat aataattcca ataaaacagt aaacgttgcg
    10381 tgcgttatat tatacaatga atttagcaat tttcttgata aatgttaata tttcttaaga
    10441 atgtagcttg aaaattaaca attacttgaa ctattacagt taaatagggt tatgtacctt
    10501 aattgaaaat gataatgcga acaaatagga ttgataaatc aacaaaaaat atactaagtg
    10561 aagaactctt atttagcttt gcaacattat aaaaaaacaa tacatgagga aagaagcaca
    10621 atattttgat gatatcgtac aagcacgacg ttctattcga atttatgatg ataaggccac
    10681 atttgacgag acagcagttg acagaagttt ggagcgcgct ttactttcgc caaattccag
    10741 caatttacaa ttatgggaat tttacagagt gaaatcaaaa gccaagaaaa atgaacttgc
    10801 agaatattgt ttaggtcaaa atgctgctaa aacagccaat gaactgattg tatttgtgac
    10861 cagaaaagat aaatggaaag aacgtgcaag atttgtttta gccgaagcaa aaaaagaatt
    10921 tccagctaca ctgagtaaaa aacacaagtt aattattgag tattatgaca agctgattcc
    10981 gactttatat ttttcggatc cagtagatat tgcaggtggt ttcaaaaaaa ttgcttcagt
    11041 agtcagtggt acatttcaac ctataccacg cgaggtgaca gcaacagata tgcgaattgt
    11101 ggttcataaa agtgttgcat tggctgcgca aacctttatg ctcagcatga aagctgaagg
    11161 ctacgacacc tgtccgatgg aaggctttga tagtgtgcgt gtgaagaagt ttttgaattt
    11221 accacgtggt gccgaaatat gcatgatagt atccgtaggc actgccaagc ctgaaggtgt
    11281 atatggcaag cgcgttcgta ttcctaaaga gcaagtaata tttgaaattt aatggaacca
    11341 ttaatcgaag gtttgatagc tggcataacc gttagctttc tcataggtcc aatatttttt
    11401 gcattagcag aaattacgat gacaaaaggc tggcgttgtg gattagctta tgtttttggt
    11461 gtcatcatta gtgatgtagt attgatttat gccattgaaa aagtactgaa tatatttcca
    11521 tttgatgatg ccttaaaatt taaaattgga ataattggtg gcatcttgct tatcgtattt
    11581 gggctatctg tgtttttgac caaagccaac ataaaacaat atgatgtgac caatgtaaaa
    11641 actttattag gtgcatttgc caaaggtgta actatcaatg ttttcaatcc ttttgtgacg
    11701 gtgtggtgga taacgatgta ctctacaatt tcaattaatt atattgcgtt gaatgataaa
    11761 atcgtatttt attttgctat actattgatg gtttttttgt ttgatttatt gaaaatgcga
    11821 tttgcctatt atctaaaaca aaagctgacg gttcaaaaat taagtatagt aaaaaaaggt
    11881 gtaggcattt gtctattcat ttttggcctt gctatgatta ttaaagtatc atgattaaaa
    11941 tggtgttctt aatattgtaa tttcaagttt cttttataaa caaaaaaaga tgcattacat
    12001 tactcagcat cttccatatc atcaagcatg atgcttttat ggttgacata gtcatcaacg
    12061 gtgttgaatc gaaagtctgg atactcgctc ataaattttt tattctttaa accaccttgt
    12121 agcggtcgtg ctgcaggtgc tgcaatttgt gtcgtagttt gtggtattaa ttcatattct
    12181 gcttctggaa agtattttaa aatcgtcaat gccaattgca cacgattcat ataatcagtg
    12241 ccagcaatat tataaattcc agatttgttg tcgcgcaata ataaatacat acaacgtgct
    12301 atatcagcag catttattgg tgttgcgtat tggtcaatag gcaaacgcaa tgtaagcttt
    12361 tgctgttcaa aaatttgatc gatgatacgt gatacaaaat tttttccacg ctcttcatcg
    12421 ccatatacat tagtaattcg caatactaag ttttgtgcat ttgcagcaag tacagcttgc
    12481 tctgcttcta atttatgctt accataaaca gaaattggtc gcacaattgc ttcttcatca
    12541 tacggaccgt tttctccgtc aaatacataa tctgtagaaa taaaaacaag cttagcttgt
    12601 aattcattag caagtgtaat cacatttttt gtggaaacaa cagtcttttc atagctttca
    12661 gcttcgtttt gctcgcaata atcaacatgc gtgagtgcgc cacaatgcac gataacgtta
    12721 ggtttgaatt gtgttacgtt aaaattattt tcgttatcga gattcaaagt gtcaaaaaat
    12781 acagtattgg cagcttcata agagaaatat gtaccgacac aattccaatc tttttgttta
    12841 aaatatcgta aacaattatt gcccactaat cctgatgcgc caatgatgag tatcttcatt
    12901 tttatttatt tattttatat tttgagtaga tgtaatgaga tatttgaatt gtataatgaa
    12961 ataaggaatt atacatgcac tttatacctt acaatactaa actaccattt tatttgatta
    13021 aattttttaa tacttttcac aaaatacaac catataatac tttccttgag tatgccatat
    13081 ttattaggct ttccgatggc gattgtggtt tgcaattttt cattttcact tcgtttttta
    13141 ttccattttt cgatgctact tttgtactca tttaatcctt gtcgaatagc ttgtgcttct
    13201 ttgatatttc cttttgctaa tgccactact gctgccaatt ggtcgagcct attgcgagtg
    13261 aaataaatag attgtaattc gttttctgtc aaattctttc ttaataattt tctgttgttt
    13321 ctaaaattca acaaagtttt tctaggattt gatttgttta atgtgccacc gccaacatga
    13381 tacaccttac tctgtgaaca atatgcaatt ttataaccta agttttttaa tcgccaacat
    13441 aaatcaattt cttccatgtg cgcaaaaaaa tcagcatcaa agccaccaac tttgtgaaat
    13501 aaatctgctt gtataaataa acaagcacca cttgcccaaa atatttctcc atcgttgtcg
    13561 tattgccctg cgtctatttc acaggtgtca aatattctac ctctgcaaaa cggataacca
    13621 tatttatcaa taaatccgcc tgcagcgcct gcatattcaa atttattttt ctcgtgatag
    13681 gataaaattt ttggttgaca tgcagcaata tttgtatcca gctgcatata gctaatgatg
    13741 ttttctatcc agtttggtgt aacttctacg tcggaattta gaagtacata atagttgctt
    13801 tttatgtgtt gcaaagcttc attgtaacca cctgcatagc cagtgtttac tgagttttgt
    13861 acaattttta cgctgggaaa tgatgcttgc agaaatggta ttgaatcatc tgtagatgcg
    13921 ttatctgcaa ctataatttc agcatttgga taagttgtat tttctaaaac atttggcaga
    13981 aatttctcta accaattttt tccattataa tttaatatga cgactgaaac tttttgcaat
    14041 tgcgatttta atttttagta atacaatatg gacaaagata cgatacaaaa attcgaatat
    14101 caataaaata aactaatagc ttatgccagc tcgcttccat cttcgatggc tccaaagcca
    14161 gtattctggt ctttgttgaa tgtcgctttc taataatcgc acgtattttt ctacgatttc
    14221 attttctgct gtttgactgg cattttctgt tatcgtttca aaacttagtt cataatatgc
    14281 tctatttatt tttcgtatcg aagcgtataa aacaatagca tcagtacgac gtgctaaggt
    14341 gtcaaatcct ttataaaatg gtgtgttgat gctcataaat tttgtccaat aagcattttc
    14401 tgtatcaaca ggagtttgat cggcaagaat ggccactaat gatgtttgtt ttaacaaatt
    14461 tgcatatagt gtttttaatt gatttgcagc gactaattga cagccaaagc gagcacgatt
    14521 ttttaataaa aaatgttcga atgtcttatt gcttggaggt gaatacaatg catatgatgg
    14581 taatttgctt gttattccag caaggagaga agcccattcc caattgccat aatggccaag
    14641 tacaacaaaa atatgttgac ctctattttc ataatttttt agcaatgcat aactgtcatg
    14701 ttgcagttgt aatcgtgctt ttaattcgtt ttttgaaatc gaaatacttt tgattgtctc
    14761 tataattaaa tcagcaagat gcctataata attgcttcta ataaaccaaa gttcttcact
    14821 ttttttatgt ggaaatgcat tcattaaatt caaatcgatg attgtttttc ggtaagcata
    14881 tatgcgatta aagatgaaat aaataatgtc agccagtgta aacaataagt ttaatggaaa
    14941 ataggaaagg agtcttgcgt agaatattcc aatcttagtg agtagccatt gcatttgaca
    15001 aaattaagga taaaaaatgt atctgttact ttcgttgccg aaatcgaaaa tatttatgat
    15061 tgtattgcac attagtttta ttaatttaat cgtatatagc atcgtataaa gttaaaatgc
    15121 taatatcttt gccgtatgag tgctatttta atcgaatcgc aatatatagg atcttgtacc
    15181 tattggcatt gtttgctaca agctacatca attgtcattg accaagaaga acattatgtc
    15241 agaagaagct ttcgcaatcg agcacatata ttaggtgcaa acggattatt gcgtttaagt
    15301 attccattag aaagaggcaa acatcaacat tctaaaatga aagacgtcaa aatttcttat
    15361 caagaaaatt ggcaggcatt gcactggcat agcattacat cgtgttatag acgttctcca
    15421 tattttgaat attatgaaga ttattttaaa ccattttaca cagaaaaaca agaattgtta
    15481 ttggattata atttaagttt tatgcaaacg attgctggtt tattaaaaat aaaattgcct
    15541 atatcaaaat ctgataaata ttatacaagt gctgatttta ttggcattga cgcaagaagt
    15601 tattttctac caactaaaaa tataactatt gattttccaa catatcctca agtatttaat
    15661 gatagatttc cttttgtgaa agatttaagt ttgttagatg tgttgtttaa tgttggaccc
    15721 aatacatcaa actatatttc atctcttcct tttaaatttt gatatccatt tctactttcc
    15781 agagttcctt tttttcgtcg ctgtcaaatt cttttttcaa ctttacatcg atgttaaggt
    15841 tgaccaatga aaagtcggct acttcctttg gttttagatt atatgatttc gagtcttgca
    15901 caatattttc agcctttgtc aattgcacat ttttgatatt gtaatttcgc aattgtaata
    15961 ccaaataatt gtacatacct tctgctttag atctgtcttc tgtttttacg aagctacaat
    16021 tgtagtaata acggcctttt ccgttgttta tatatacaga ttcgtaagcg cctttgatat
    16081 taaaattgca atacactcga tctttggcat ttggtatttt tacagagtca atcgagtttc
    16141 tgccatttac tttcaattga ccaattgaat aatttactgc atctaagaaa ttgaaatatt
    16201 gataatcagg cactgaccac catgtcggat tcatgtctaa agtaacacga atacgattga
    16261 tttcgtagta tttttcaatc gcattatagc gcaacacttt taggctcatt ggtgtgccaa
    16321 cattgccttg taccaattta agcacatcgc cgtagcccat tttatgaatc ggaattttat
    16381 caatttctaa aattaaatcg cctggtttta gtgtttttga attgatataa tgattgattt
    16441 ctaatgattt tactttaaac cgttgtgctg tagaatcaaa taccgcttgt ccgccaatgc
    16501 caccaatttt tgatgttgca cttggcgtag tggcagctac aaacaaaaag aaaattccaa
    16561 taagaaggct agtagttttt ttcattgctt acaaactgca aatttaattc caaatgctta
    16621 caaaagaaag ccttcaatgc ttaattgaag gctatttgtt tgaatttcaa ggttttttca
    16681 catttttata actttttaaa caatgcaccg aactccatca tcttcattaa atcagaaact
    16741 tgtatttttc cgctcataaa tgcttcttgc ggatttatct ttcccgtttc cactgcgatg
    16801 tatgtttctg tttgagtagt aattattaag tcgtaagtat caagattttt ttcttttcta
    16861 attgatatat ttccattttc tatcataaca gcatatcgca aatcattttc aaattcaaac
    16921 aatacattta tgttttttcc atttgctttt tcttttttaa atcgagatgg cagcgtgtta
    16981 aaaattccat caatatctgt cggaatttgt aatgtagctt gttgtgaatt ttcagaagtt
    17041 gctctgttgt aattggcatc atcaattacc atttttgcaa ttatctcgcg cataatttct
    17101 gatgtgccac cgccgattgt accaatccta gaatcgcgga atgctcttgc aattttgaag
    17161 tcttccatat agccgtatcc accaaaaaat tgtaatactt gataggctgt tttgtcggct
    17221 aattctgaag ctaataactt ggcaatggaa ctttctttaa cggcatattt tttttcatta
    17281 tgcattctgc aagtttgcaa tacataaaac tttgttgctt caatttctgc tgctaattga
    17341 gccactcgat gacgcaacac ttgaaattta ttgattggtc tgccaaatgc ttcgcgctgg
    17401 ctcatatatt ccatgccata ttgcagcatg ccttcacacg ccgaataacc cattatggag
    17461 ccagctaatc tttctaattg caagccgccc attaaatagt aaaacccttt accttcttcg
    17521 cctaataaat tttttgtagg tacttttaca ttgtcaaaat gcaattcagc agtgtcagaa
    17581 gcatgccaac ctaatttatt taatttattt tttgaaatgc cttcgctgtc taaatccacc
    17641 acaagcaaac ttacgccagc tgcacctgca gctggatttg ttttgcaaac taagattaca
    17701 aaatggccat aaataccatt tgtgatgaag gtcttactgc cattgacgat ataattgtcg
    17761 ccttctttta ttgctttagt ttgtatgttt tgcacgtcgc ttcctgcgcc tggctcagtg
    17821 atgccgatgc aaccaatttt ttcaccggca atacctggtt tcaaaaatgt ttctttcaaa
    17881 taatcactac cgtgatgtaa aatgtaaggc atgctcatac aggtttgcac agcagccgta
    17941 attgcaaaac cacctgagaa tatttttgat atctcttcaa tcatgacaac cgtataaaaa
    18001 aagtctaaat cactaccgcc ataagctgct ggataagcta atccgaaata gcccatttcg
    18061 ccgaattttt tccaaaattc gcgtggtatt tttccatctt tttcccaatt gtcgatatgc
    18121 ggtgtggctt ctttttctaa aaaatctttt atagattttc tgaatgtttc atggtcttcg
    18181 ttaaagtagt aagataatgc tgacatattt aatgaaataa tttagtaatg aaataattta
    18241 agaatgaagt gatttttaat gatataattt aataattatt acgttatttt ttttgatgta
    18301 cttatgattt tagctaatat tttcttgatt tcatgaaggt tggtaattaa attatcacaa
    18361 tcaggataac tctcaaccgc tttacaaatt tctaaccagt attctgtttc ttctacttct
    18421 tttaatgcta cttttatttt atgaatgaaa tcggatttac tttcagcatt ttgtgcttct
    18481 ctgatatttg cgccgataga agttccagat tttaataact gttttgaaat aacatatttt
    18541 tttctcgttt ctaataattc acaatacaaa actatttgaa cagaaaattt aaatgttaga
    18601 tttagaatag gattatcttt tttctccatt attcaattat ttcatcgatt cattattaaa
    18661 ttttcggaac attgttccga actgcatcat ttttgataaa tctgatactt gtatttttcc
    18721 ttgcataaat gcttcttgtg ggtttaattt tcctgtttcc acttcgatgt atgttgctgt
    18781 atttgtagtt ataattaaat caaaatttga ttgatttttc cctttttgaa ttgataaatc
    18841 ttctttatta attaaaatag aataatgtaa attttcttca aattcaaata agacattcat
    18901 ctcattttca tttacttttt cttttttaaa tcgtgctggc aaagtcgcaa acaattcatc
    18961 aatagtaata tttttaatct catttctcac ttcccgtttc tcataatcta tatcatcaat
    19021 taccattttt gctattattt cgcgcataat ttcagatgag ccgccaccaa tagtacctaa
    19081 tcgtgaatct ctaaaaaatt gagcggcttt atattcttcc atatagccat agccaccaaa
    19141 catttgcaaa cattgataag ctaatttatc tgatagttct gttgctaaca gtttcgacat
    19201 ggaagcttct tttacacaat attttttatc gttatgcatt tgacaaacca gataagtgta
    19261 tgcttttacg ctttctactt cagcaaataa atcagctatt ttatgtctca atacttggaa
    19321 tttattaatt ggttttccga atgcactacg ttcgctcatg tattgcaaag tgtattgtag
    19381 cattagctcc gacgaagaca atgcacctaa cgctaatgtc aatctttcta attcgaagcg
    19441 ttgcatgata taataaaaac ctttgttttc ttcgcctaat aaatttccta ttggtacttc
    19501 gacattgtcg aaagcaatct ctgcggtatc actggctttc cagcctaatt tatttaagtt
    19561 atttcttgtt aatccttttg aattaccttc caccaatatc atagaaattc cacttgagcc
    19621 tgtatttgct gttttacatg ccacaatgat ataatctgca agtacgccgt ttgtgataaa
    19681 acatttgctt ccattgatga tatatttatc gccttgtttt attgctgatg ttttgatacc
    19741 tgcaacatcg ctacctgcat gaggttcagt aattgctaat gcacccacaa tttcgccacg
    19801 aatggcttta ggtaaatatt tttcttttaa ctgttcagat gcttcgtgtt ttaaatgaga
    19861 catcgataaa tatggatgtg cagaaatgct tgcaccaaag ccaccactcc agcatttact
    19921 ggtttcttcg atgaatatga catcatacca aaagtcgaga ttagcaccgc cgtatttttc
    19981 ttcttgcgtg ataccgaaga agcccatttc gccaaatttt ttaaaaattt ctcttggcaa
    20041 aaaaccatct ttttcccatt tttctatatt aggcattgct tctttatcca aaaaatctcg
    20101 taatgttttt ctgaatagtt gatgctcttc tgttaaatag tattgttcta atgctgtcat
    20161 tgtttttagt ttattttttg caatttcaac atttctcttg tttctgaaat gctggcaatt
    20221 tctctatcta acatacgtgc taattttgcg ccccattcta cgcactcacc atttgacttt
    20281 gccatttcgc catttggtaa ataaaaatta tcttctaagc caacacgaaa atttccgccc
    20341 atcgtacaag cagtagccgc caactgccat tgttttctgc taatgcctat cacttgccaa
    20401 aacgcacctt ccggcacttg cttgatttga tgaatcaaat tttctgttgt tgcaggaatg
    20461 ccacctaaga cgcccatcac taagctataa caagcgttgt caggaattaa gcccatttct
    20521 cgaagaggct caacattata aatatgtcca gtgtcaaaac attccatttc aggacaaata
    20581 ttattttcat tcattgtttt taccactttt atcatcgtgt caaatgagtt ttcaaacacg
    20641 gcattccaat aaaattgttt ttgttttttt gaaaagatag catagttcat acttcccata
    20701 ttgaatgctg ccatatctgg ttttgttact ggtaaatgct ttattctctc ttctacggaa
    20761 agtccaatag caccagtaga aaagttgata attacatcag cagaatgttt gcgtacttct
    20821 gcttttattt cttcaaacac ttccgttctc cagctcggaa taccgttgtc ttctcttgca
    20881 tgaatatgaa cgatgcttgc gcctgcatct acagcacgtt tggcttcttc gcctaattct
    20941 ttcggtgtgt aaggaattgc tggacaatgt gctcggttag ttgcagcacc agtaagtgca
    21001 gcactcaaga ttagtttttt gtttgacatg atgttatttt atttcttcgt ggtttaaata
    21061 attattcgcc tttccattct ggttttcttt tttctttaaa tgctgtaatg ccttctgctg
    21121 catctttact ttgtaaacac tgcattagca tagcatgtaa gtatttgtgt ttctcatcag
    21181 cattttttgt tttcatttct tggaatgctt ttaatcctaa gcgtatcgct gttggcgatt
    21241 gctctttaat ttctgcaatt aattcttgta ctctcgatgc taaattttct tcttttacaa
    21301 cttcagatac aattccaatt tcttgtgcct ctttagcatt caaaatctta gctcttatgc
    21361 ataaatctaa tagctgtctt gccgacatta aaggctctaa tgttgccatt acttggaaag
    21421 ggaagatgcc gcgttttact tctggtaatc caaattttgc tgactcaaca gcaataacat
    21481 gagtgcatcc accaatcatt agaaagccgc cagcatatac atctgcatgc acttgtgcaa
    21541 tgcatggttt gtgtaaacca ttaaattcat cgccgagttt taccatgtct tttggcatag
    21601 gaatggtcga ttgtttttct gtagtatcat cgccagcaaa agcttttaaa tcggcgccgg
    21661 cacaaaatgt tggaccatta gcttttaata ccacacacca aatagcattg ttataatgtg
    21721 cgtaggcgag tgcatacgcc aattcattag ctaatggagc atgaaatgca tttcgtttct
    21781 caggacgatt caatgtaatc gttagcacat tgtctttttc ttcaacgatg atgtaagcga
    21841 agttttgttt ggtgaagttt gatgtttgtt cttgagtgta tatcattatg atttattgtt
    21901 ttaatttatc atgtcattct cgcgaaagcg ggaatctcat tgtatgttgt ttaatttatt
    21961 ttttttattt cttttttgtg agattccctt ttacaaggga atgacattcg tttaaattca
    22021 aaatttattt ttttatgtat caattttttt gttgttaatg tcattctcgc gaaagcggga
    22081 atctcataaa tctatataaa atattttctt gattgattgt attcaattat atctttttcg
    22141 ctataccaat cctttgctaa atcaattcgt tctatattca ttgtatctat taactcgtct
    22201 ttccattttc tattccattt ctttaattgt ttttctcttt caattgcttc catcatattt
    22261 gtaaattctt caaaataaat tagtttatta attttatatt ttttcgtaaa aacagaacca
    22321 tcgccttgtt tatgttgtaa aattctattc cataaatcag atgtaactcc aatataaatt
    22381 acatttccga atgtattgga aataatataa atacatggtt cttttttatt tattttcatt
    22441 tcctattttt ttatgagatt cccttttaca agggaatgac atacgtataa attcactatt
    22501 cgtatttata tctaccaatg tttttttctt tcgtgtcatt ctcgttaaag tgggaatctc
    22561 attgtatttt gtttgttgat ttctttgagt ttcccttctt caatggaata acgttgctat
    22621 tttttacaat gctacattat tacattactt cattactgca ttattctttc attttcaaca
    22681 acaatgttcc actttctaca aattgttttt ctgttacata tatttcttct acttcgccgt
    22741 cgctgtgtgc ttcaatggtg ctttccattt tcatagaaat catttttaaa agagcatcgc
    22801 cactttttac ttggttgcct tgttttacta atacagttgt aatatcgcca ggcataggcg
    22861 cgatgtatgc acctttcatt atttctgtct gtggtgctgg aaatcggtct atgctatgta
    22921 atacaatctg acctaattga ggtgattgta caaaatattg atgtccgttt tgcgagatgt
    22981 agaaatttct acgaatgttg ttgatttcaa acgtatatgg ttgctgagga caccaagcat
    23041 tgctcgattg ttgaatgcct tttgttgatt gtcgatgagt agaaataaaa tctttttctg
    23101 caaatgaaat atgaagctca ttgtttatat ttctataaga aataggaaat tcgaaacctt
    23161 gaaaactata tttttcttgt agcgcttgat agaaattatt gcgccatcca ctaggtacac
    23221 cttgtgctat attccgattt tcttcgcgct ctaaatatct aaaatgaatt aaggcacacg
    23281 ttaaaatatc aagcgtttct tgtgcatatt gttcgccttt ataattgaat tgtttttcta
    23341 aaaaatgtgt atcaaaattt ccattttgaa aatcttgatt ttctaaaatg gaaatcaaaa
    23401 agtttttatt ggtagtaaat ccactacaaa ctgtttcttg taaggcagat tttaattttt
    23461 gaatggtttc tattctattc tttccttttg caattatttt tgcaatcatt gggtcgtaat
    23521 aggtagaaat ttcagcacca ctttcgattc cagtatcata acgcaatcca tcgttaatag
    23581 caggaatcca atctagtatt tttccattta ctggtaaaaa attattactc ggatcttctg
    23641 catataatcg cacttctata gcatgaccgt tttgttttat tacctcttgt tgaaaaggaa
    23701 taggattgcc ttgtgcaact tcaatttgta agcgtactaa atctaagcct gttacttctt
    23761 ctgttacagg atgctcgact tgtaaacgtg tgtttacttc caaaaaataa aaggattggt
    23821 ctggagcaac aataaattct actgttccag cattgtcata attgattgct ttgcatgcag
    23881 caattgcggc actactcatt ttttgtcgag tttcatcaga gataaatggc gatggacttt
    23941 cttctactac tttctgataa cgacgttgaa tactacattc tctttcaaat aaatgcaatg
    24001 cattgccatg tttgtcgcca aaaatttgaa actctatatg tcgtgatgta tcgaaatatt
    24061 tttctatcaa tagcgtatca tcaccaaagc catttagcgc ttctgatttt gcttcagcaa
    24121 ttgctttgtc caattctttt tcttctcgca cgatgcgcat acctttgcca ccaccaccag
    24181 cactagcttt caataaaaca ggaaatccaa tttctattgc tttggttttt atagtagcat
    24241 cgctttggtc atcgccttga tagccaggaa tagttggtac attatgctgt tgcatgattt
    24301 tttttgcgcc aatttttgag cccattactt caattgcctt tatatgagga cctacccaaa
    24361 taatgccttc ggaaataaca cgctgtgcaa attcgacttg ctcactcaaa aagccatagc
    24421 caggatgaat ggcattagcg cctgtttttt ttgcagcttc aattatttta tctatcacca
    24481 aataagattc gttaggttgt ttaccaccaa tacgcactga ttcgtcagcc atttttacaa
    24541 acaagctatt ggcatcagca tcagaaaata cggctacagt agaaatgccc atttctttgc
    24601 atgttctgaa gatacgtatt gcaatttcac cacgattggc gacgagtagt ttgtttattg
    24661 agtattgttt attgagtatt gagtattgag acatttgatt tcagatttca gatttcagaa
    24721 ttatgatttc gtacttattt ctttacagat tttaatttca aagtttttag gattgcacta
    24781 aatatagctg ttaattcgtt tgcttcttta agtaatatat ccaaatcctt taatttataa
    24841 ttagtttctc ctaataattc aaaccagtaa atacattcgt cagcttcttc ttgtacaatg
    24901 tttattttat tgataaaatc aggatttgat tttcccctta atgaagaacg ataatttgcc
    24961 ccaacagatg tagcagctct tatgacttgt ttaataatta taacatttga ataagttggc
    25021 ttaatacttt ctgtgaactt tatggtattt actgcaaatt tctttgtcct attttttaat
    25081 tctgttgagt tcattatact aaatcttaac tcttaattct gaaatcataa ataataaatt
    25141 ccattacatc ctataaacac cataactact tgttcctttc acttcattca tatatgttaa
    25201 tgctaatacc aaacctaata cattgcgtgt gtctcttggg tcgatgatgc cgtcgtccca
    25261 gagttgtcct gatgcaaaaa aagcattact ctgtttttca atttctgctt tcataaattc
    25321 tttcatttgc gtagcttgtg cttcatcgta ttgttgtccg ctttttatag cagcttcttt
    25381 gctgaccatt tgcattacgc cagctaattg ttctgcgccc attacagcga tcatggaatt
    25441 aggataagag aataaaaagc gaggattgta tgcacgtccg cacatcgcat aatttcctgc
    25501 gccgtaagaa gcacccatca ttaatgtaaa cataggtact tcggaattag aaactgcatt
    25561 tatcatttta gcgccgtctt tgatgatgcc attttgttcg tactgttttc cgaccataaa
    25621 tcctgtaata ttttgcaaaa acaaaatagg tgtgtggttc atgttgcaca attgtatgaa
    25681 atgcgcacct ttattagctg tatcattgaa caatacacca ttattgccaa ttattgcaat
    25741 agaataacca tggatttttg caaaaccaca tactaatgtt gtaccatatt cttttttgaa
    25801 ttcatgaaat tcggagccat ctactatgcg agcgatgact tcacgacaat caaatggaat
    25861 ttttacatct acagatacga taccaagcag ttcatcaatt ggataatttg gctctttata
    25921 agaaatttca gatttcagat ttatgatttc agattttaaa tttaaattag acatcagttc
    25981 tcttgcaatt ctaattccat ctaattcatc ttgtgctaaa taatcagaaa caccggaaat
    26041 tttagaatgc atttcagcac cacctaaagt ttcatcatca acaatttcat tagtcgccat
    26101 ttttaccaat ggaggtcctg ctaaaaatac ttttgcttta tcttttataa aaattgtgta
    26161 atcgctcatg ccaggaatat aagcaccgcc ggccgtagaa ttgccgaaaa caatagatat
    26221 tgttggcaat ccttttttag aatgtcgtgt aatattttta aaattattac cacctaaatt
    26281 aaatacttgc gcttggtcgg gtagattggc accagcactt tccactaaat ttatgcaagg
    26341 caaattattt tcgcttgcta tttcgctgat tcggatggct ttttttaaag tggcaatatc
    26401 aatagcacca cctttgtttg tgccaacgtt agcagtaatc atgcacattt tattggaaac
    26461 aaatccaatt ccagcaacca tagtaccacc tgtgccaaat ccacctttca caccaagtcc
    26521 agctaaagga caaagctcta aaaatggaga atcttgatcg agtagcattt ctatgcgttc
    26581 tcttgccaac aattttcctt gcgattttgc tttagaaata tgtttttctt caccttgaaa
    26641 aagtgattgt ttcaagtgtt tattcatttc ctctattttt gacaacatct tctgataatt
    26701 gtctttatag gctgctgaat ttgtatttat tttagtttgt aagatggaca tgttatcgtt
    26761 ctttttttgt attataactt ctatttttaa gaattttgta ttaaacaatt atacaaaaaa
    26821 tatttgataa acgaacgttc gtttggtatg tatttttaat cattatttca ttttcatttt
    26881 taataaacag ataatcaggt gtttatgatt taatttaaac aaacgtttgg taaaagtttg
    26941 ttagtttcag atttttattt tacatttgat aataaacttt ttaatactta aaattaaata
    27001 aaatgacagc aaaagaaatt atgttaggat tgcctgcgcg tttaaaaaca gaagaagcag
    27061 caggacaaag cggaactttt cattttcaat tagaaggtga aacaggtggc gattttacgg
    27121 taagcgttgc agatggtgca tgtacagtta cggaaggctt atcaggagag gcagattgtg
    27181 taattagtgc agcagcaaat gattttgaag atgcagaaga aggaagagta aataaacaga
    27241 tggccgtaat gatgggcaaa ataaaaataa gcaacttagg tgctatgctt aaatttatgg
    27301 gtttgtttag cgatttggaa gcagcataat cctaatttag gatgcgaatt aaaaaggaca
    27361 attcaagctt gaattgtcct tttttttata caaattctca ctcagaagta tcgtttacta
    27421 gtttattgtt gacaaatttt caaaaacatt gaaactttgt tgtacaaaat tataaaacga
    27481 tgaaaacaag aacaaaaatt aatgtattag tgctgtttat agcattgatt acatcacttt
    27541 ttacagtatc ctgtaaaaaa gaaaaagcac tagattcaaa agattattca aacatcaaaa
    27601 tcgctaattg gcgagattat attggtgatg aatttggcat tgaaggctac atcattgacg
    27661 aaggaaatgg tcatccgcaa attatttcag atgatgaaaa ttatttgttt gatggattat
    27721 ttccagaatc agaatattta cacttagaca tggccagcag aaacgatgac ttaagacgtt
    27781 tccatggtcg taaagtaaaa attaaaggta tattggaagc caacacagat caatcgattg
    27841 taggcactgc aagctttttg ggcgacccat cattagccac tttacgtgtt agctctatta
    27901 atgcaattcc tattgatagt gtatcctatt ttagacctac tactaattgg aatttctgcg
    27961 aaagatatcc taatgtttgc tctacttttg ttacaccaat tggtattaag tgtgcgattt
    28021 tatatagtgg tggtataaat gcttcgaaag catatacacg ttattggaat gacatcaaat
    28081 ttatgtataa tatcttaatc aataatggtt atttagcagc taatatccgt gtagtatata
    28141 gagatggtat aggaaatgat ggttctattc cagttcatta tgcagccaca ccaacaggct
    28201 ttaatgatgc ggtagaatac atgaaaacta aattaaattc gcagtctaaa ttatttttaa
    28261 tgtttaacaa tcacggtggt ggttatgaaa catcaacagg aaacaattat ggcgtgcttg
    28321 atgccaataa tgacgaagca gaagctagca ataaaactga tgaaaactat tgttattacg
    28381 gcgtttctgc accgttttta gacgatacga ttgctgcaaa aatcaataga cttccatttt
    28441 atgaattaat tgcagtagtt aaaccatgtt tcagtggtgg tttaatttgg gatttgcgcg
    28501 gtgctaatag aacaatcatt acttcaggta ctgagtttca ggtgacttat ccaaatcctg
    28561 gtggtcagtt tggtgaattg tcgtataatt tctttggcgc agttacaggc atgacaccag
    28621 atggcgttgt agtagatgca gatataaata atgacggaaa agtatcgatg tatgaagctt
    28681 atattttctg tagagataat gaaagacgta atgagcaacc acaatttgga aatagtgctt
    28741 ctggcacacc aacaacaaca ccttcttcgt ctggttacgg tagtaatgtg tttttataag
    28801 atttgagaaa tagataattt caatgcagca atttattgct gcatttttta tattagatac
    28861 aattgatttt gtactttcgt gttacataca aatagatgga atcttttcaa ataaaatatt
    28921 cagaaggtag aaatttggca ataatcataa taacgttgat gttagtaatt gctgcattag
    28981 taggtgtaat gttgtttgct tttctgaaaa tggatattga tggtgatatt ggagcaatta
    29041 tatttggtgt cattataaca ttatgttcaa tcttattagg tgtcgcactt atagttatgt
    29101 ttaaagtaaa aggagttgtt tctttttctg aaaatcttat agaaattaat ttgaataaat
    29161 caacattgtt atataaaaat acaaacataa aaataccatt ttccaatatt aaaaatgcag
    29221 tgttagaaaa cgacacttat aataggaaag ttatttcctt gtcaatagtt gctccatcaa
    29281 aaacgattta tttagcgcca attagtaatg atgaaaacga taattttgat caattttgga
    29341 ataacctaga tgcattaatt caaaaatata atcaatcgca aaaaaacata ggacaacaaa
    29401 tccaacataa aagtatgtat gagcaatggt gggcaaaatt attagccatc atttgcgctg
    29461 tattagctat tggtatatgt attgcaaaaa tagttgacca agatgcaata tcaacatggc
    29521 gtttaattgc ctttttagcc tatactattc cattttgttt tattgtgtac caagcaaata
    29581 aaaagaagaa ataaatatga aagataagat tgtcgtaatt actggaagca actctggaat
    29641 tggtagagtt gcagcaacag aaattgcaaa aatgggcgct caagttatta tggtatgtcg
    29701 caatgaagca aaagcaaaag ctgttcaaga agatatcaat gcagtaacag gtttgaataa
    29761 ttgtgattta tttatttgtg atttcagctc acacgcatca ataaaacagt ttgcaaaagc
    29821 cattagaatg aaatacaatc aaattgatgt gttgattaat aatgctggtg taataatagg
    29881 tgagcgcgaa gaaaatgaag aaggcatgga aatgatgttt gccaccaatc atttaggtta
    29941 ttttttagtg acacattatt tattagactt attaaaagct tctgataaag ctcgtattgt
    30001 aagtgtagct tctttggcac atcgttttac taatatgaaa tggaataatc tacaaggaga
    30061 gcaatcattt aattcttgga tacaatacgg acataccaag ctgatgaata ttttattcaa
    30121 taaagaatta gcatatcagc ttaaaaaaaa taccaatatt acggccaact gtttacatcc
    30181 tggaaacatc aattcgaact ttggacaaag tggaaatccg ctgatgaagt ttttaatgtc
    30241 gaatttcggt tttgtattga ctacaccaga gaaaggagca gagactacaa tatatttggc
    30301 gacatcgccc gaagtacaag gaaaaacagg cttatattgg tatagcaaaa atccgacttt
    30361 tccatctttg gaagccatta gctctgaaaa ttcgcgacga ctttgggaaa ttagccataa
    30421 actttctggt gtgaaagaat ttggtgttgt taatttagat taaacaatga caatataaaa
    30481 tggatatttt ttcatttttt acaacaaatt ttccgccact ttcattaatt gctcgcttgt
    30541 ttccaatttc catactttgg agctttgcag cattaacttt tgcaggatac tgcaaaaaga
    30601 aatttcattt caaaacagga tatacaagaa aaatattcca ttttttaatc tttatttccg
    30661 cagttttctt tcaaatattt tattctttac aaggcgtatt tattttaggc tggtcagtga
    30721 ctgtagtatt gatatttgcc tgttacaaag gcaatggttt tatgttatat gaagccatcg
    30781 cacgcgaaaa agatgcacct tatcgaacaa aatatattgt gtattcttac ttggctacat
    30841 ttttaggtgg cgtgttttca aatattttat ttggtcaata tgctatcttc ggctacgcaa
    30901 taactggcgt tgccgatgcc atcgcagagc caataggcac gcgctttggc aagcatcaat
    30961 atcgtgtttt tagcttcgat aaaatgaagg tttcttacag aagtatagaa ggtagtttgt
    31021 ctgtgttggt ggccactttg cttgttattg tattgctggc aacaacattt ttttctgtct
    31081 ctattccatt ttattatatc tgttcaattg ctattttatg catgctggta gaagctgttt
    31141 cgccaagtgg ttttgataat ttattattgc aattatgtag cagttatgct tgttatttta
    31201 ttatacagct actttaatca tctttgcgtt ttgcattatt tttatcaata aatgtgataa
    31261 agtcatacag tttttctaca ggaatatttt tggcaacaat tttataattt ctgtcgataa
    31321 tgaaaacttt tggagtagca ataacatcat attcttttct aaatgcactt acgccgcgca
    31381 tatctgcgca ggtaatccaa tctgtagaac aattctttgc agcaaatgct ctaaatgagc
    31441 tgtctgtttg ctctgtagat acactgaaaa catctagacc aaattgtttt aaagagtcag
    31501 tataaatttg ttttaacaaa ggaatttcct tttggcaatg gccacaatct gagttccaaa
    31561 aaactaaaac ggtgtatttg tttttgctaa taaagtcatg aaaaatttga ttctttccgc
    31621 ttgtatcttg tacaataaaa ttgggtgcaa tttttcctat taatgttggt cgaatggctt
    31681 cgactctatc tctcaattcg cttaagcttt tttcgctcgc ccaccaagcc ttgcctgtga
    31741 aaaaatattt gtcagacatg tacacatata ttgcatcatg tcccataatt tcacttttcg
    31801 catacttcaa gaaaatttca ttcaaacaaa actgaaacat ttcagcattt gcttgtgctt
    31861 tatctaatac aaaatcgatg gattgtataa tagaatcagg ctgtggaaag gtatatacat
    31921 caaaatactt caacaatttt ccttgaaaaa ttggacttcg aataaatcca gaatcagcaa
    31981 aatctatgtg gtcgaaataa tgttgtttag tatagtgaaa tgtataaaac gaatctatta
    32041 actcaccttt tgcatttttt ggtgcttcag gtatttccaa ttctaacatt gcctttatca
    32101 gcttagaata aaaagttgtt ggatattttt ttgctatttt ttttctatgc gaatttatgt
    32161 cttcacttat attatctaat tgatcggcta tttttttata ggcatctgaa tttttttcta
    32221 atggttttaa caacttttgc aaggaatcat tttgcacacc taagggtatc atataattca
    32281 tatcttcaaa caattgcttg ttttcaacag aatttttaat tactgcatgt ttgatgaaat
    32341 tgctcgtgtc tgtttgaata ctgaatgctg tttctttgat gatgaaatca aaagaagcat
    32401 aacgcatact tggaaatgcc attaaataca cgccagcagg tatattttta ttgcctttga
    32461 tttgtgctac gcctttttca ttaaaatgca atgtatcttt caagtatttt ttgtcaccat
    32521 aataattagc gagaagacca tcttgatttt ccattccttt aatggtgatg gtgatattga
    32581 aattattttc ttgtgcaaaa gtgatagtag tgaaaagtgt tataaaaagt aatgtaaaaa
    32641 gtggcttcat catgcgtttt tcaaattgat ttcaaaattc gttccgaaat tactcattcg
    32701 actataatta attgtataaa tctttgttaa tttgtataaa acaatgttga agtatttata
    32761 catagcaggc ggtggcgcac tcggtacaat ttgcaggtat tggctggtaa catgcttaga
    32821 gcaaaaatat acaagtacat ttccttttgg cacactcacg gttaacctat ttggatcact
    32881 tatgattggt tttttattcg gtctaatgtc agcaaacggc attattgaag aaaaatggag
    32941 attgctattg tttattggat ttttaggtgg ttttaccaca ttttcttctt tctctttaga
    33001 aaatatgaaa ttgttgcaaa atggtttttc gagtacagcg attgtctata ttatagccag
    33061 taatcttttt ggtattttgt tggcatttct tggctatttc atttcattga aatttaaata
    33121 aaaagcgaaa ctcaaagagt ttcgcttcct gtataatatg tgttaatact tatttgaatt
    33181 gaaaatttgc gtcagttagg tttacaccta attttaaatc cttgaattca taaatttcat
    33241 aaattccttt ctcgtcttcc attttgtgat agataggcaa taagtttgat ttgtcgatat
    33301 ataaggttgt tttctttgca taagaagaag gaattttcaa tttttgacca ggttttacat
    33361 catcgtcaat atcatttaat tctttgattt tatactcaga gatagccaat tttttaccaa
    33421 tttgccatac cgatttttcg cctgctgcta ctgtataatc aatgatttta taatcagcat
    33481 cgttgatttc aatcttccag cattcttggt tgtcataggt aactgtacct aaaactttta
    33541 cataagtagt taagtcttct cctttgcgtt gtgccatgct tgtttctaaa atctttttaa
    33601 tttgtccgaa acctgctcta gtaactggat tatgaacgcc tttcattaat aagctattgg
    33661 ttggacttaa attcaattta aaaccttttt tcacttttac atcagtagat acagcagcat
    33721 cataaaacaa ctgtgctgat tgtggtttgt gtgcattagc atatattttt aaaggcgaag
    33781 cgattactgt aaattcaaca tcacttttgc tgtattttcc atcgtgcata cgctcttgcg
    33841 cataaaaatg ataggtcatt gttttggcat ttcccatagc tgccatcacc ttaggaatca
    33901 tttcttttac attttgagca aaagaattat ttgccaaaaa aagtgtggca aacactataa
    33961 gactggttaa tttcttcata ctttttaaat tattatggac taaaatacgt tgttttgacg
    34021 agatattaaa atttttaggc aagaagtttt tcaatcgttg aatttatctt ttgaaaaggg
    34081 aattgcgcca ttgtattctg catcatcgct tcaatttctt catgacataa gttgccaata
    34141 cttccttcgt gcgtatgatg aatcacactt ggttttttgt aatttcctgt ttctacttgt
    34201 ttgccaaccg taatatatgc atcacggaat ggcattcctt cgtttactaa ttgattcacc
    34261 gtttctaccg taaataaaag tttgtatttt tcatcttcta gtatgttgtc ttttatttca
    34321 atatgtgaca acatgatatt gctcatttcc aaacaggaaa taatagtaga aaaactatgc
    34381 atataatctt cttttatgat ttgtaaatca cgatgatatc ctgatggtaa gttggagaga
    34441 atcaaattaa attgatttgg taggctttgc agcttattac attttgccct aatcaactca
    34501 aaaacatctg gatttttttt atgtggcata atagaagaac cagtagtcag cacgtcagga
    34561 aaggaaataa atccaaagtt ttgattcaaa tacaaacagg catccattgc tagttttgca
    34621 agcgttgcag caatatttgc aatagcagca acgacggttt tttccatttt tcctctattc
    34681 atttgagcat acacgacatt gtaattcaag tcggcaaaac ctaataactc agtggtcatt
    34741 tttctgttta atggaaatga gccgccatat ccagcacctg aacctaaagg atttttatta
    34801 gccaatgtaa atgcagcata catcgtttcc atgtcatcta ctaagctttc ggcataagca
    34861 ccaaaccaca agccaaagct actaggcatg gctaattgta aatgcgtata accaggcaat
    34921 agtttgtctt tatgttggat agataattta agaagcgtgt caaataattg ctgtgttgca
    34981 tttgcaattc gctctatttc aaaacgagta aatattttta aatccagtaa aacttgatcg
    35041 tttctgcttc tgccactgtg aatttgtttt cctagattgc caagtttttc tgttagtcta
    35101 aactcgatat ctgaatggat atcttcactg tctttgtgaa tgctaaaatt tcctttttca
    35161 atgatagcat aaatacattt tagttcttga tgtattggtg ctaggttttc ttgtggcaac
    35221 aaaccaacag cagccagcat ttttgtgtgt gctaatgaac ctaatacatc ggcttttgca
    35281 agtagcgtat ctaaatttct atcatcgcca accgtgaatg tttctacttg ctttgctaat
    35341 tcgttttcgt ctaaattgcc tttttgccaa agttttgcca tgtcaataaa tcttcttata
    35401 aagatgcgaa gaaataaaat agaatcaggt aaagaaatat ttatttttta ggtgctactg
    35461 ctgtttctaa agcaggatat tcgagcttca tagcttttaa agcatcgcgc actaataatg
    35521 caatctgata ttctttgtac caattttggt caacagcaat gatattccat tttgcagctt
    35581 ttgaacagtt tctaaatgca tcttcatatg cattgcgata ttgcttccaa agctcgcgtt
    35641 ctttccagtc attatcgcga tgtttccaca tttttgcagg attgactttg cgttcttcta
    35701 atcgttctaa ttgagcttca ggcgaaatat gcatatagca tttaatgatt ttggtgccgt
    35761 tttgttctaa taatttctca aaattattaa tcgcatcaaa gcgttgtttt accgtgtttt
    35821 cgtctatcca attgtgcaca cgttgtatga gcacatcttc gtagtgtgag cgattaaaga
    35881 ttgtaatttc gcctttttgt ggtgctactt gatgcacacg ccatagaaaa tcatgtgcta
    35941 attcttcttc tgttggtcgt ttaaaagttt gtacgcgcaa gccagcagga ttgattccgg
    36001 aaaatacttt ttttatagca ccatctttac cgcttgcgtc cattccttgc aaaatcacga
    36061 gcagacttct cttctgggat gcgatgagta catcttgtaa ttctgcaatt tgcaaaatca
    36121 actctttagt ttttacttta gtcagttcct tatctaggga tttaggtgca actgttgcta
    36181 ttttcgagag tttaatttct tccataatta ctatttagct gaatctatat tatttgaaca
    36241 ttccttttaa tgctcctaat aaaccacgtg taatggtagt tgtagcggtg cgtgcaattt
    36301 gcttagtgac tgtgctattt aatactttat ctaaagtgct tttctgagtc ttagctactt
    36361 ttgcatcaat ttttgcttgt ttttctgctt cttttgcttg ctgctctgcc acttcctgtt
    36421 gttgttcaat ttgctgtgct tgctcaattt ttttatttaa aatttcataa gcactctctg
    36481 ggtcgattgt gtctttgtat tttttataaa gattggattt ttgaatcaat gttttcattt
    36541 cttcttcatt caaaatatcc atacgcgatt gtggcggcgt cattaaagca tgaactaatg
    36601 gcgtaggaat acctttctca ttcaatgcgg ttacaaatgc ttcaccaata cctagttgcg
    36661 taatcaaatc ttctactttg taataacttg tttctggata gttttgtgcg accaacttaa
    36721 tggcttgtct gtcttttgca gtgaaggcac gcaatgcatg ttgtactttt aatcctaatt
    36781 gagctaatat actttctgga atatcatttg gattttgtgt acaaaagaaa ataccaacac
    36841 cttttgagcg gatgagtttg atgatagctt ctaattggtc ttgcaatgct ttgctggcat
    36901 tgtcgaaaat caaatgtgct tcgtccataa aaataaccaa ctcaggttga tccatgtcac
    36961 cacgttctgg aaatttagca tatacttctg ctaataaact caacataaat gttgaaaata
    37021 attttggttt atcttgtatg tctgtaagtc gaacaatgtt gacatagcca taaccatttt
    37081 catctttgcg caataaatca ttgacattaa aagacgtctc accaaaaaac atggctgcac
    37141 cttgttgttc taattcaacc acttttcgca atattgttcc tactgtagca ggagagatag
    37201 caccatattc tttttcaaaa atatcctttc cttcatttgt caaaaattgc aatacttttt
    37261 taaagtcagg caagtcaact aatccaaaac cttgatcgtc acagaattta aaaaccatag
    37321 caacgacgcc ttgttgcgtg tcgtttaagt ctaatatttt tgaaattaaa acaggaccaa
    37381 attcagtaac tgttgctctt aaacgaacac cgtcttgtga cgaaatactc attagctcaa
    37441 ctggatatgc ttttggattc cacgtgtttc caattgctac aactctgtct gttatttttt
    37501 gattggaaac acctggttct gcaatgccag agaaatcacc ttttatatcc atcattaaaa
    37561 cagaaacacc attttctgat aatttttcag agatggtttg tatggttttt gtttttccag
    37621 ttcctgttgc gccagcaatt agtccatgtc tattgagtgt ttttaatgga atatttatca
    37681 aagtatttgc tatcgatttt gcatctaaca tagcaccacc aacagttatt gatgtgcctt
    37741 tgaattggta tccttgttgt attgtctgaa taaattcttc ttgacttgcc attttgtttt
    37801 ttttggtaaa tgtaaggaaa tgttgtaatt cattaaaaat tataatcaat atattataaa
    37861 aaatgaaatt aacattagca ttactccaag taggaaatga atgctacatt tcttcctgtg
    37921 ttttataatt caaaatgtgc acctaattca ggtatttcta ttgatttata attttcttgt
    37981 aataatgttg ttttaaatgc ttcttgacgt ttgggttcgc catgtactaa aaatattttt
    38041 tttaattttt ttttatcttg atttgctaga aactgcaata attcaggctc atcggcatgt
    38101 gcactcatgc tactcattat ttcaatttgc gctttcactt ttttttcctc accaaatatt
    38161 tttatacgtt ctggcttttg tgtgagctgt tgacctaaag tacctacagc acaatatcca
    38221 actattaaaa tggtgttttc tgctttgtct atttggtgca acaaatgatg tttaattcgt
    38281 ccagcatttg ccatgcctga tgctgaaata atgatacatg gctcgtcaga tgcatttagg
    38341 cgttttgagt gttctgcatc acgtgtgtaa tgcatgttat tccagccaaa tggattttca
    38401 tcagtgcgca tatattgatg tatttcttca tcgaaacatt ctggatgcac gacaaatatc
    38461 tcagtggcat tcatagccaa tggactgtca acaaatacag gaatgttttc taattttccg
    38521 gcagtatata gcttgtctaa gcgatataat agttcttgcg ttcgtccaac actaaatgct
    38581 ggaataatta atttaccacg tttttcaata catgtttcta cgattatttg taaaaaatgc
    38641 tcatcgctct ctggcgtttc ttcatgtttt tttccgccat aagttgactc acaaatgaga
    38701 taatcgagtg caggcattgg cgatggatct tttagtattg gacgattgta tcgaccaata
    38761 tctcctgaaa atccaaacgt gatttcctcg tttttttctt taatgtttag tgttacagat
    38821 gcactaccta agatatggcc agcatcagca aaaaatactt tcacattatt tttaatgcta
    38881 aaccaaatat catattcaac agtgcgaaaa tatttcaaac aatttttagc atctaattgt
    38941 gtatataaag gtttttgtgg ttttctattt ttcttggaag cgtaagcagc atcactttcc
    39001 tgtatgtgtg cactgtctaa caacataatt gccgctaaat cgcgtgttgc agaagtgcaa
    39061 tatattttac ctcgaaatcc atcttttacc aatttaggaa ttcgaccgca atgatcaatg
    39121 tgcgcatgag ataaaatgag aacatcaatt gcagaaggat taaaatacca ctttgaatta
    39181 aaatcttcaa attcatcttc atgtccttgg tataaaccac aatccagtaa aatggtaaaa
    39241 ccatcttcga gtgtcaatag atgacagctg cctgttactg tttgagctgc accacatatt
    39301 tttaatttca tatcat
//
LOCUS       DAOQQV010000012        37396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_313_length_37396_cov_8.458183, whole genome shotgun sequence.
ACCESSION   DAOQQV010000012 DAOQQV010000000
VERSION     DAOQQV010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 37396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37396
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_37396_cov_8.458183"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1076)
                     /locus_tag="PLL59_02585"
     CDS             complement(69..1076)
                     /locus_tag="PLL59_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMY22719.1"
                     /translation="MKVAVYLPETTPYSMKHCAWNIMSILQEKEKVHFVTITTLEDVA
                     TTDADIYWDPRCGGGIAPALKFRSLARPLILTVHGMAMFTLPIDTFYFSWQQKISGQI
                     KCWKERLKWTLMQKHIAHIMTVSEYTKSELIQTIDFPTAKITAVWNGIDHHKFKPAQK
                     IETTSPYFLTIISYQKKKNFERLLEAYQLLDEASRPRLVALVKPYEVPHTVKQIKGLE
                     IINYAIDEQKIIELYQQALGLVFVSLHEGFGLPIVEAMACGTPVITSKTTSCKEIAGD
                     AAYLVDPTNTVAIRDALQSLALNKTLREELSKKGIDRAQLFSWEKSAAAFYKILLSCK
                     I"
     gene            complement(1078..2430)
                     /locus_tag="PLL59_02590"
     CDS             complement(1078..2430)
                     /locus_tag="PLL59_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HMY22720.1"
                     /translation="MAFSNYKEKFQSALRDKNFSFVLKGSLSTAITLFFVQGLRFVSG
                     VLIGRFYGADASGKLTLVVTVMGIFAIFVNFGVKEALQKLIPELREKYNLKTAYAVFL
                     KGNQIILLLSFISAIILYFISPWLANYWNEPDMVNLFRISSLFIPFFVLGELNYFSLR
                     AALKVHTANMSLVVPTIIRIAVLLLITFYFFNTYNPIYLHWSTLCVLPWLFSLIPVYQ
                     FFHKPSAAIDTVSETSHQQIITVAFPMLMTYLAFAVNNSADVFILKANDIGTDAVGIY
                     KTCTNISMLAATLLVALNTTVQPKMTQLYTQQNYDEVRRISVKSSKLIFLLSLPVFIV
                     LLFFSKYVMCLYGDEFMKGAMCLSVLTLGQIVNTVCGPVAQLMNATGHHRQFRNISFI
                     GMAINILANLLLIPQFGILGAAIANSVSMALWNIVATFYIKKHFGFYIVYIPFINLNK
                     "
     gene            2533..4044
                     /locus_tag="PLL59_02595"
     CDS             2533..4044
                     /locus_tag="PLL59_02595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMY22721.1"
                     /translation="MLFNSLHFLLFFPIFCYLYFSVAKKNQWKILVAGGYYFYGNFSP
                     FFAIILFVITCNDFLAGKMMDKASEQSKKKKWLFLSMAGNLGILFTFKYLDFADNNIR
                     ELLAVFNIPWFVPEPYFNSWIVPVGVSFLTFQSLSYTIDVYKGVTKAEQHFGYFAGFT
                     AFWPVMVNGPIERAKNLLPQIKEEHRFDYDRMRHGLFRMAWGFFKKAVIADRLAYFVD
                     NMYLHYHEISGWTTIIGGFFFFMQVYCDFSGYSDIALGAAKVIGIDVMENFKRPLFAK
                     NLADIWTRWHISLSTWLTDYVFFYLGAYKASGAKVIFNVIFVLAICGLWHGANWPMVL
                     AFTMVGVFMAIRYWWQANVVRAIKPSNSYRLFDKYFPNWLHSVITVALFVFCFLLFRV
                     HTAQLAYNSSHPNAPVEWASMAKTIYYNLFKIHTAGYFHGWITHQGIVQFCVVVFFML
                     ILISTEALIGDRKIETVVLAKNKQQRWTIYILLLISIVWFGIFNNTSFVYFQY"
     gene            4048..5127
                     /locus_tag="PLL59_02600"
     CDS             4048..5127
                     /locus_tag="PLL59_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22722.1"
                     /translation="MFKKTIIHIAIIVLILFISAAIVRFFMPFYWGDATQTVKYEYYE
                     KNAEKYNAVYLGGSLEYRHIDPSIIDSVAQKNGIDFHSFNLGIDGHGIIQQLNDLEGL
                     LKIRNPNLKYVFLSISSESYFFKANLHTPKWISWHTATSTYNALNLTSTLDDPLKFRT
                     KFSGFWLSSWIENLFNIGTVPDMLKYKFDKSYLDTSYVGKNKDGFYPYDYEESHLFME
                     YKWEDTLLLESRHRFEKSDPYTDTLNKSVLNSFTNYKTTDAPNKVMTALYLDAYKKCK
                     KKGIQVFFVLPPKARTSYSLLLPVFNAMPEGSKIELADIRKYPKFYSIEYGYNFHHLN
                     YKGARLCSQELAKQLIPLLKSEENN"
     gene            complement(5124..5624)
                     /locus_tag="PLL59_02605"
     CDS             complement(5124..5624)
                     /locus_tag="PLL59_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HMY22723.1"
                     /translation="MLRAIAKIIRDILLTIRRWFFMFIPEQSYLYMACGGINLVFDMS
                     LFFISYHFIFHERNFVTPFFTFSPHIAALFFSFMISTGTGFFLSRYVVWTNSLVRGRN
                     QMINYLTVVALCVAMNTGFLKLFVEVFQFRLFMARMLTAAIVITTSYFLQRYFAFKVH
                     KAPKMV"
     gene            complement(5658..6326)
                     /locus_tag="PLL59_02610"
     CDS             complement(5658..6326)
                     /locus_tag="PLL59_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HMY22724.1"
                     /translation="MNNKIDIFYIQHTQELIDAVFQRQLVGLPQIFQNEINAYKHMES
                     AQASLLGKLLLKHAFQNLQVQYSLHDVVIGRKDRPMLNDALDFNISHSGEFVICAIAQ
                     DAKVGIDIEKHRNINIELFKKYFNDEEWNEIKTAKNQNLSFFDMWSLKESAIKCDGRG
                     VEILSKTHKQYTATDEHQIVCDDVLFHYKKLQIVEQYSAFVCSNMPFSLKITNLHYAD
                     LLSS"
     gene            complement(6355..7059)
                     /locus_tag="PLL59_02615"
     CDS             complement(6355..7059)
                     /locus_tag="PLL59_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMY22725.1"
                     /translation="MKAILLHHAGGDKYAFRNLQQNLLPEIESIAFELPGRSDRISEP
                     FIKNIYQAVDDIYEQLIRKMPNEYCIIGNSMGAAIAYLLAHKLQQENKQMPKHLFLSS
                     RLSPDAYKQEPNIVGISSTDFWKVVQQYDGVPAQLLQHKELMEFYEPILRTDFELLQQ
                     FNESFESIAAIDVPASILFGDQDVRNVSNEKMQGWRKFFVNNIDIKSFEGKHFFLYEN
                     ENVATYIKMKMNVVSR"
     gene            complement(7067..10324)
                     /locus_tag="PLL59_02620"
     CDS             complement(7067..10324)
                     /locus_tag="PLL59_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HMY22726.1"
                     /translation="MSEQLQQMQEILQKSLIKIKKLELELAQAKENIALPKEPIAIIG
                     TGVRLAGSIDSTKKLWNLLSAQRSVFSKIPKDRFDVDAIYSADVSIPGKTVAQFGAFL
                     DNDVRAFDADFFGISPREAKSIDPLQRMILEVVYEAIENAGIASDTLKGSNTGVYIAL
                     GNSDYIQARFRSGSLDTVDVYDTTGIPFGTACGRVSYLYDLKGTSFALDAACASSLLA
                     VHLANEDLNKKIIDTAIVASANLLLTPEPFVGLSKLGSLSPNDACNAFANDANGYVRG
                     EGCGVVLLKRLQDATNDNDNIEAIIKGSAVKHNGRSNGFTAPNPEIQIETIKEALKNA
                     ELSVNDIDYVEAHGIGNRFTDALEIQAIQDAYTGRTKPIYVGSVKANIGHLEAGTGMP
                     MIFKVIEILKHQQIPAQINIAALNEDVDWNTIQAKVNTESIKWQSDIAKPMLAAINLS
                     GYSGTNVHMIFEAAPPKSKSDEVPSPLHFVLSAKDAHALKSLANKYLSETDWQENSLT
                     EICFTLQNHRNNFDYKLSIVANNKQAIIEGLTDFVQDIKSEKYAVSQPSLNRNKEVVF
                     MFTGQGAQYFGMCKSYYDTFDIFKQTIDECDEMMKSALQISIKDILWNDSIDKNLIHQ
                     TKYTQAALFVVEYASAKLWMSFGVHASALIGHSIGEIVALTIANAIDLKDAVQLVIAR
                     AALMNSLPMHEGSMASVFCNEETLQPFLENSNVNLAAVNSIKNCTISGKKEDVATFVA
                     MLQEHKIKAVPLQVSHAFHSYLMEPIVEKFTIFAQQFSFKQPAITVVSNLTGKEITKA
                     DLSAAYFANHIRHAIQFAKGVDYLQKNNYEVYIEVGPNPVLISLAKQSNTNSNALWLP
                     SAKREENDITYFHKTLQQLYCADIKINWSIFYLHKKINRVALPSYAWNQKVYWENPVR
                     QAIHFAHSSNTHTSNNKIEIHDSNYQTASSNTQAIATRETLLAYMQIEAAKVLGLEAG
                     QKVDINKPYREQGFDSMMSGEYLSLMEKHIGAELKMEIIHQYNTPKDLHQYLIDTYYG
                     GGTVDTTQAITMADIMFNQEMEHSFQGDWHEIQATDNALMRWFKKIDKKLPTVKN"
     gene            complement(10362..14903)
                     /locus_tag="PLL59_02625"
     CDS             complement(10362..14903)
                     /locus_tag="PLL59_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HMY22727.1"
                     /translation="MENIDAYQQQLSELRQQISVLQSALENEKNASNEPIAIIGMAMR
                     LPGKIKNAKDLWRVLVNGIDCIEEIPANRWDKDALFDADPNAPGKLYVKEGGFIEDIE
                     YLDGAFFNISPIELESTDPQQRILLEVTHEAFENAGIDVNKLVGSDTGVFIGIDNVDY
                     EAKQIRSKDYKLISGYCYTGTGPTGASGRISYTMGLRGPCMTIDTACSSALTCTHVAI
                     QALRNKDCKIAVVGAASIISEPDQSLNFCRLGALSPEARCKSFDDAANGYIRSEGVCA
                     MIIKKLSDAQNDDDNILAIIKGSAINQDGKSKRLTAPSTAAQGLMHTAALKNAQLQPT
                     DIDYIEAHGTGTKVGDPVEVRGLMLAYEKFRTKEKPLIIGAIKSNIGHMECNAGMASM
                     FKVVLGLQHNIIPKSIHFHTPNTLIDWENIPIKVAKDNIEWKRENDKIRYAGVSGFGA
                     TGSNAHIIIGDAPKKINTTQKDELRHDVYVLPLSAKSEHALFDLAKLYATFIQESEYK
                     LEDICAMTSLRRAHFDLRETFVATTKAELIEKLNDYAENGNYENKKKFDTKEKIKTVF
                     IFPGQGAQWITMGKMLMQHEPVFKSALEECAQVFSYFVDWNIIDEINKAEASSRLSEI
                     DIVQPVLVAMEIALANLWMSKGVFPDSVVGHSMGEVAAAYVAGMLTLHDAAKIICTRS
                     KLMKQLSGKGEMGATDLTAEEANEILKAYENRLSVAVMNSKNSTVISGDPAALDEVFA
                     KLEAQGRFNRKVKVNVASHSTQMDEIKQDLFDALQTIQPQNSTIHFYSTALNEIIEGD
                     KLKADYWVKNLRNPVQFGNAIRAIVQQEQVAFIEMSPHALLTHAINENIQDKPAIVVA
                     SITRDKNELIEFYSNIASLEASHYPFDWKNIYPNLGAFVQLPNYAWQKERYWFDQQPE
                     YSINTSIVKKDISKNLYNINWHEIQVAPNIEAKKILIVKDNYGIAHAVENVLRQHACI
                     VTTIDAQDDFENIDVDIVIHVGSLYNDNPFLLNLENGILSLQKMLHQFNRSNKSPKIC
                     VVTNGAFTLLNDTAANLNSSMMTGVLRTLENEHEEINFLQIDIANELKDIDSKQLASL
                     VFVDDVYKEIAIRKQTCYASNIEKISTLPKRSKSIKQDKTYVVVGGTSGLGLYTVNWL
                     VQQQAKNILVLSRSGAKEEAKSIFEKYKSEGVYINDVIADVSNFASLQDAISSAPSVA
                     GVFYAAGILDDAAFENLSKAQFESVITTKAIGAWNMHELTKNQSLDFFVLYSSAAGIV
                     GSAGQANYNAANTFMDSLAHYRSANQLEALAVDFGTIAAIGLAARQENRADRLAEQGV
                     TAIQPNELYAYFDTLFLSDATQIMAMEIDFSKWSNFNHAVLHNHFYSKVVQATHEEQQ
                     AESISFANIDEYKKHIKEHIKQHISTATKLLVSKIKEDETFKALGIDSLHALQLKNKL
                     QADFNLTFNVAVVWQYPTVQKLADFIANELKLNEQFANESSDVISSTPSSAGLDDIET
                     QVKNLSLEDLMKELSSKVD"
     gene            complement(14973..16061)
                     /locus_tag="PLL59_02630"
     CDS             complement(14973..16061)
                     /locus_tag="PLL59_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22728.1"
                     /translation="MQQQFVEEDIRLVDVLESFRSYLIYLLKKWYISIVGVVALTAGG
                     YYFAKLSAPQYVANISFNALDSRASAMGGLMSMMGVSFAGGSSNDVLTGIFTSRNIFL
                     NSMIEDVEINGKKDKMANFYFHALKYDDGFEKDSVLKDFKFSANSVEEISKTEMDFFG
                     MMYDDYFDGLLTAEYDVMSGVIRAELETPDYEVSRTLGSVLLKKTTEFYQARQLENAT
                     TTFKSTSKRLDSISGEIAYRQRLIAQSQDQNIFNRKRIETVDQQKIMQELAVLGALYN
                     DASSSKENAKVGLTPASNIVRVIDDPMFSTAPKRKSKLLFAAVGFAVSILLIILPLIM
                     QKAINDGREEDKVKAAKLAAQETPATTT"
     gene            complement(16069..18495)
                     /locus_tag="PLL59_02635"
     CDS             complement(16069..18495)
                     /locus_tag="PLL59_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HMY22729.1"
                     /translation="MKKLVSLCLLLCMIYTSFAQNLGNLTGAMTGTSITTAAANAAKQ
                     LQTLTSTSATSNPNLSQLQLLGLDPSTIQKYLKQKSEADNAGSQGQMQDVLQDLLQMR
                     IRQDSMQVMMERMEKAKAMTQDVKPNEIFGHDFFGSGKLALFSKSADSKAPDSYILDV
                     NDEINIAVWGYADYNNKLKVNEDGFIQIPEFGRIYVKGLTFGAVKAQIGKRLATFINP
                     SNTKYEITLNYSRTIDVNIVGEVDMPGTYQIPAINSVYNAINAANGITEIGSVRDIQV
                     RRDGKTIKRFDVYEFLFNPLLQENFFLQKGDFIYVSTQSKLVKIAGAIRRPAKYELLK
                     GESLNDLIKFAGGLSSNAYTKSIQVSRINNDKAEIIDIDYDQLVNSNFELKDGDVVTI
                     STIPNTVENTVSIVGSVRYAGKYELKAGYRISDVIKIAGGIRLETYLERAYIKRKLDD
                     NTYVNKKFSLKNILIDENSPDNVLLQSFDQIQLFSKDEFIERFFVGIDGSVIKPTILE
                     YTEGITLNDLLFYAGGLKKEAANSKIEISRVINIDSSAEQIFVPQRTVVQTVTVGVNL
                     EIDEASKAYVLSPMDRVFVRKTYGFDDQLVVTLKGEVKYPGVYPILDKNEKVLDVIER
                     AGGLTPYAFIKNARLTRPDNSLNQSIFHLKDAFADTASRANLILKGGDIIEIPTVNQM
                     VSIKGAIRYPGLDSTLTISGKFVPGKSARWYIKNYAGGFKKGALKKSTMVIYPNRKVD
                     MTRSFIGIKNYPTVDNEGALITVDMKVKKPKPPRGPESALNWNIVLPSVIAALTSIAS
                     TITLVFVLKK"
     gene            18711..20102
                     /gene="mnmE"
                     /locus_tag="PLL59_02640"
     CDS             18711..20102
                     /gene="mnmE"
                     /locus_tag="PLL59_02640"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HMY22730.1"
                     /translation="MTYHTDTIVALSTPPGLGAVGVIRISGTRAIEIVNAVFKGKNLL
                     QQASHTLHFGKIVHEQQIIDEVIVGIFLAPTSYTKENIAEISCHGSPYILQKVIEVLI
                     KNGARSAKPGEFTMRAFLNGRFDLSQAEAVADLIAADSEIAHKTAMQQMRGGFSLEIK
                     RLREELIHFASLIELELDFGEEDVEFADRMALSATIEQLLRTIYALSASFKVGNVIKK
                     GVPVVLAGRPNAGKSTLLNALVNEERAIVSNIAGTTRDIIEVPINIDGLTFRLIDTAG
                     IREASDTIEAIGVKKTFEQIENAAILLYIFDANDLSELEVLEDINKLNNSKLTIVLVA
                     NKCDLCQEHTLGTLENSINKLTKNAFPIFEISAHKQQNIHQLKAYLTSALGINSYLND
                     NTLVTNIRHFEALNNCYLSLEKVSLALQNKITTDFLAQDIRHALSALSEITGSITTED
                     LLDNIFSKFCIGK"
     gene            20273..21058
                     /locus_tag="PLL59_02645"
     CDS             20273..21058
                     /locus_tag="PLL59_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMY22731.1"
                     /translation="MAQAQTKKNSGGLGAGLVVLIAIIICNILFYTVFKSQLDEKGHE
                     KNIIGLFYKGGVIVPFLMATFITCITFAIERLLTISKAAGTGSLDTFLQNIKSKLVSN
                     DINGALAECSKQKGAVANVVQAGLHKYAAMEKDTELNGEQKIMNIQKEVEETTALELP
                     ALEKNMWILSTIASVGTLIALLGTVIGMIKAFAALAVNGNPDPAALSSGISEALVNTA
                     LGIFTSALAIIFYNLCTQKIDKLTYGIDEMGYSISQTFASRKH"
     gene            21083..21721
                     /locus_tag="PLL59_02650"
     CDS             21083..21721
                     /locus_tag="PLL59_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMY22732.1"
                     /translation="MPKFKVKRGSTWVDMTAMCDVSFLLLTFFILTAKFRPAEIVPIT
                     TPTSRAENAVAEDMVTFSIDKDGKAYMSIGKPERKTQIIEKLVSLSQNKYGNIDENTQ
                     KLFTRLELVGVPIQQLPQAVKRSTAELTEMRVAGQLSGIPMDSTNNQLGDWVQAVRYV
                     YAETDKLSNPPVAIKGDRASNIKGIKRLIEIFKEKDVYKYKLVTSLENKVEK"
     gene            21733..22302
                     /locus_tag="PLL59_02655"
     CDS             21733..22302
                     /locus_tag="PLL59_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMY22733.1"
                     /translation="MADIDTSSGGGHGKHKGGVRSKKLSTRVDLTPMVDLAFLLISFF
                     MLTTQLSKSVAMDLAMPKKPDNVEVKPEPVAGSKVLNLIADKDDVLWYYPDVVVGGLK
                     KTEYTPSGLRQVILKRQAEVKAKWGLDNDGDSKMVVLIKLTDDANYKNMVDILDEMEL
                     TKTKIYAIQDVNDLELEAIKNGGNANISE"
     gene            22324..23160
                     /locus_tag="PLL59_02660"
     CDS             22324..23160
                     /locus_tag="PLL59_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMY22734.1"
                     /translation="MSNNNNFDYKNASWDDIIFDNLNKDYGAYVIRKLVNKHTLRGFG
                     IALAGFLIIILSLRFNIFSFKNKKEKIVDLEMTDVQLEQAPPPVEAPPPPPPPPPPPP
                     VRPTIKFIEMVAAKDEEVPEEREPPKQDNLTKDDQNVGEKDQKGSLDASPLVDKGTDN
                     PAPAPEPEQPKEPEIFDRAEVMPTYPGGVPELMKFLMKNIRYPSLARENGLEGKVIVK
                     FYIDTDGTVKDPTVLKDNVGGGCGDEAIRVVKAMPKWTPGSQRGKAVKVYYTLPVTFK
                     LQ"
     gene            23221..23427
                     /locus_tag="PLL59_02665"
     CDS             23221..23427
                     /locus_tag="PLL59_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22735.1"
                     /translation="MNSFLQPAFLLRFAIAIAYIILGIVLIVLPIDIKLLNNTTKPLF
                     SLLLIAYGTFRLYRAFQLLKEEEK"
     gene            23429..24349
                     /locus_tag="PLL59_02670"
     CDS             23429..24349
                     /locus_tag="PLL59_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMY22736.1"
                     /translation="MQYFTYFSRFISIIILLLTSSCNTQNKTEQQHINERHKGNVVIT
                     ADINLRYILEQLASIYHIYYPDANISFKYTTEDDAVSNFKLKKNDIIAVEKLLSPDEI
                     ANAQFHHQAKVLENIFAYDAIAIISNKQNSDSIFILDKLENYLNDKSKKIVFDNKQSG
                     IAKQLMQYSNTSAEAFKNAYAVNSVNEVLQFIEHNNDAIGFIPYNSFSNRNEKTAQQI
                     RTQFNILPISYHDTMYTLSQESIATQNYPLIRPIVLYIGNCPELVAQGFANFIFGKQI
                     SKALLLSGLVPKNIPIREVIVTEEFHPNKK"
     gene            24425..25681
                     /locus_tag="PLL59_02675"
     CDS             24425..25681
                     /locus_tag="PLL59_02675"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HMY22737.1"
                     /translation="MSEDKVTITLGGKTTECPVLVGTENEYAINIDKLREDTGFVTLD
                     FGYKNTGATTSKITFLDGEEGILRYRGYEIADLAEKSNFTEVAYLLLYGELPTAAQQA
                     EFNQKLKDHSDVPAEVGAILKALPKGTHPMAQLAAVTVVLSGVYTDGAKLSDENFINI
                     LAKFPVLVAMVIRNSQGLDFIANDKSLDYVSNFLHMTFGTKASAVLVEAMDKLLILHA
                     DHEQNCSTSTVRMVGSSNANIYASISAGIGALWGPLHGGANQAVLEQLEAIEKDGGDT
                     AKFVAMAKDKTSGFRLMGFGHRVYKNFDPRARIIKKSCDEVLNDLGVQDKKLTIAMEL
                     EQAALNDDYFVSRKLYPNVDFYSGIIYKAMSFPTDTFTTLFALGRLPGWVSQWKELKE
                     NKEPIGRPRQIYLGATVRPYTDINNR"
     gene            25794..26978
                     /gene="hflX"
                     /locus_tag="PLL59_02680"
     CDS             25794..26978
                     /gene="hflX"
                     /locus_tag="PLL59_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HMY22738.1"
                     /translation="MHEVNPIIEERAVLVGIIRDNQSAEQVEEYLDELAFLAETAGAI
                     TVKTFTQKLKHPDVRYFVGSGKLEEIKTYKEYADINTVIIDDEISPAQQRNLESELKC
                     KILDRTSLILDIFAMRAQTAQARTQVELAQMQYLLPRLKGLWTHLERQRGGIGMRGPG
                     EKEIETDRRVINDKIAKLKHELEILDKQEATRRKTRGELIRVALVGYTNVGKSTIMNL
                     MSKSEVLAENKLFATLDTTVRKVVIENIAFLLSDTVGFIRKLPHHLVESFKSTLDETK
                     ESDIIVHVVDASHENFRDHINVVNETLSDLSVTKQPRLVVFNKMDLYREKYFDRFLPE
                     EIKKELSEDFEKNMRAWMNCNCVFISATNKENIEELRNTMILMVQDMYHKRYPYKSSF
                     YG"
     gene            complement(27016..27672)
                     /locus_tag="PLL59_02685"
     CDS             complement(27016..27672)
                     /locus_tag="PLL59_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family metalloprotease"
                     /protein_id="HMY22739.1"
                     /translation="MGTIELDSSAKVIIISSAIICYGQSATYELSQHIADEISTMWNE
                     ANGKVVLNENMFSVRFNITFQYNNWIRANSILQNTNPRNNYIRIEEFSQLNISFVDAI
                     GSNTGYFMLDNLYSGSTTCAHEYGHSLGLPHPTELNIIGKGAPGIMYPRGTLVDSEFQ
                     YDTNAQPGGPGGTLHPKYRKVKQDDIDNLQIAELIVKDIFVLGKFTNKYHEAHVKQDV
                     "
     gene            27739..28317
                     /locus_tag="PLL59_02690"
     CDS             27739..28317
                     /locus_tag="PLL59_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HMY22740.1"
                     /translation="MQSKIQDILKASILTKQQVLENENLLKTMQTVTDKIVDCFKHDG
                     KLLFCGNGGSAADAQHLAAEFSGRFYSDREPLDAEALHVNTSYLTAVANDYSYNEVYA
                     RIVKAKGRKGDILIGLSTSGNSGNIIEAFKVAKERGLIIIGFTGETGGKMAAYCDYLI
                     NIPSTDTPRIQECHITVGHIICQLVEEKFFEL"
     gene            28363..29022
                     /locus_tag="PLL59_02695"
     CDS             28363..29022
                     /locus_tag="PLL59_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMY22741.1"
                     /translation="MLAGGFGTRLSTVVKDVPKPMAPIQGKPFLHYIFKELQHQNIEN
                     VVLSVGYLKELIQDYFQDNYLGINIQYAIEHEPLGTGGGIKNAFSFVEDDAFVLNGDT
                     YFDIELSKIKNQECDIVLALKPMFEFDRYGTVELNDENKIISFNEKKYCEHGLINGGV
                     YYFHKSLFDKIDCDEKFSFEKDVLEKHLNDLNIQGKIFDNYFIDIGIPEDYNKAQIDF
                     R"
     gene            29051..29674
                     /locus_tag="PLL59_02700"
     CDS             29051..29674
                     /locus_tag="PLL59_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22742.1"
                     /translation="MKYILLIFLLFISVSAFCNDGLSWDDITKEEDGIVTEVDGNTIS
                     GIIKIKPCAEKVIISTKDSIIKINIVGIKNIQKGNHHISMLYFNDSVDFFFGHPIDTI
                     HTLKFYKGYNCDIYAGGGGWNGAMVTGMSINTRFSESYIVSLDGKDGVDFKRSVNGLK
                     FLEIDNYKRILKKTFIDNPKIISYIDTYKKVHFDDIPKVVEYINSIY"
     gene            29679..30218
                     /locus_tag="PLL59_02705"
     CDS             29679..30218
                     /locus_tag="PLL59_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HMY22743.1"
                     /translation="MPINIDNTWTLFLDRDGVINKNIDGSYVLDWEQFEFLDGVLETM
                     PKLAKLFSNIIIVTNQQCIAKGLLTHEQLNEIHQNMMNMIELNGGRIDAIYYAPDFAS
                     EENTLRKPKNGMAFLAKDDFPEIDFSKSIMVGDKPSDMEFGKSIGMTNIYISKNATQY
                     SLADYTITQFSDLLQVIKC"
     gene            30321..30692
                     /locus_tag="PLL59_02710"
     CDS             30321..30692
                     /locus_tag="PLL59_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY22744.1"
                     /translation="MRRTFTLILLLFASSLFFPRLSQAFSVANISAEAKFGEKDLIKL
                     YPNPMTTDATIKISSDIDVERSKVAVVFYNMIGSEVYRIAQVKEYDLKINRDIFKNAG
                     IYFFQLKIDESVVSTGRITVK"
     gene            complement(30745..31824)
                     /locus_tag="PLL59_02715"
     CDS             complement(30745..31824)
                     /locus_tag="PLL59_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMY22745.1"
                     /translation="MNHEGLKQAVYKINTASGSGTGFYLKSQNIIVTNYHVIDGNKQV
                     SIENQLQDRYIADVVYVNPEADIAFLRGKNYQPDAFIPFETVETVHSRDVVYVLGFPF
                     GMPYTITEGIVSNEKQLLDGRHFIQTDAAVNPGNSGGPVVNADGLLIGVTTAKFTEAD
                     NVGFAIPASVLKEELDTFATNTSGAYSLKCNSCKSLIVEKTDYCPNCGANIDEKIFDD
                     KEISKLSLYIETAIKNMGIDPVLARCGNEYWEFHSGSALVRIFVYDKNYLYATSPINN
                     LPQGNLESLLRYVNSNPVPPYRLAVYNNQIYISYRVRMSDVFSANATIIQQNLSELII
                     KANEMDDFFVQEYACEKTNYAKLNG"
     gene            complement(31834..33027)
                     /locus_tag="PLL59_02720"
     CDS             complement(31834..33027)
                     /locus_tag="PLL59_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22746.1"
                     /translation="MQKNLSFHQTIAGSLQSSIDPSIWDKVLAAYDKGDYGDAILNCI
                     NYINPSIEQKYANADKTTYVIPHGSILVELQITKDSFVVNAPFLYVEGAKIVPLLRQV
                     AQLNFTPLSISSIVLENEKLYFKYSCPLNVAEPYKVYDVLREICINADNYDDEFINKF
                     NAKRIQEPRVTPYTAEQKEQAWQKIQQYIVEAFDAYDQLESKRLTSYLWDVLMISILK
                     IDYYCAPQGTLRSEIEKTLSFLNSKEDYYQRLSKGKEFLKKLQQYERAKFDDDLYKIE
                     VFVPYKFRTNLDGVRNILKYAYETSEKEIKSLDFIGASCTLEYGILNLFYNHNIEDAI
                     ANELSNAMENAAGKSMQETATLLFDAVKKVMTSDAFLQSVAALPKTDDINPSPKKGFF
                     NKLFG"
     gene            33125..33784
                     /locus_tag="PLL59_02725"
     CDS             33125..33784
                     /locus_tag="PLL59_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMY22747.1"
                     /translation="MIKILIADAQYLIRIGLRNLLSSNKDFKIIGEASNSAELLQLSK
                     EYQPTVIIFDYNSDKNFVLQDLESIRKISPKSNLLVISADDSKANIYKAIEYGVISFL
                     TKECDKEEIIAAIYAAAKNEKFLCHKVIDIIIEKHMHVDNDNCKAFNLSSRETEIIKL
                     TAKGWTAKTIAGHLFLSTHTVYTHKKNIMKKLKINSSSEMIVYAIQNGLISKEEFSEA
                     N"
     gene            33891..35015
                     /locus_tag="PLL59_02730"
     CDS             33891..35015
                     /locus_tag="PLL59_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22748.1"
                     /translation="MYLLNLRNACIFAIAVLFVSCKEEKAPLIIPTTYDGANFDANVS
                     DESSLRTQLSALSSYMKRAENVLNKLEADSLTKYYANNGTPSLKSITATSYSNNIENS
                     YFPTMVTCSQNVYDVALGAVALNGGVYGARLLDKRGKETLQEVEKGLYEAALYNHLVT
                     LSQGTITTATVDKMIRIYGAHANFPNTNTIANTSTPDAFIALYTARRDKNDGTGFYTK
                     IRDQFIKLQAAVKAGSEYNDERNEAIASIKLLIEKSIMATVINYSYAAIPKFTSGTPT
                     ASSNASAIHDISESIGFIHGFKAIPAAHRKISDAQIDELLGLFYAPSNADASVYKFVT
                     NPVTETTRISDVITKIQSIYGFTNAELIDFKTNWISAQGR"
     gene            35135..36214
                     /locus_tag="PLL59_02735"
     CDS             35135..36214
                     /locus_tag="PLL59_02735"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HMY22749.1"
                     /translation="MCFAFAIACKGPESTDEFGKKEMFINYADNIILPAYTSYGTTLN
                     NLQTAFTTFSTNTDATNLSALQTAFLNAYAAWHTCEIYEKTPPAENAMAIDNNNYFPT
                     RKDSIEAYIGRNSTSLSFVQSRIKSDKGLPAMEYLLFSRTLTPTQILERFTIHPNAVA
                     YKAYLGSLISNCINVQASIVTEWATYRDTYINSLGTDAAGSISYMVNSFAQRVDDFKR
                     HQIGIPAGYQGNVATININPQAAQAYNANKSIEYMLLTLNNLKDVLNGKGAVDGKGLN
                     DYIRTLNVTSTFGGNLADDILAQIDVCIDKVNACGVDYANTITTDKPKADALFLEAKK
                     LLVLMKVDVPSALGVSITYTDSDGD"
     gene            36270..>37396
                     /locus_tag="PLL59_02740"
     CDS             36270..>37396
                     /locus_tag="PLL59_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HMY22750.1"
                     /translation="MIANVKNFLYRPVSIAPLASFRILFGAMLLFSSVRFVAKGWVDL
                     LYIQPQFFFAYEGFEFIKPLLGNGMYFVFGGMILAAICILFGLFYRVASIAFFFLFTY
                     VELLDKTNYLNHYYFVSIISLMMCILPANKHASLDVKLGITSTSTYIPNWCIFILQLQ
                     IACVYFFAGIAKINPDWLLHAQPLTIWLKSRDHYPIIGFLFNYSIVPYLFSWFGMLFD
                     ISIAFFLWNNKTRPFAYIAVVVFHVITSYLFQIGVFPFVMIVATWIFFSANFHQKFLS
                     IFSIAPIQNKVIYIPKFNSFFVALLCFHFLIQLLLPFRYALYPNKLFWTEQGYRFSWR
                     VMLMEKNALTTFVVKDANGKIEEVCNRNYLTPQQEKQMSTQP"
BASE COUNT        12175 a         6622 c         6324 g        12275 t
ORIGIN      
        1 ctattcttat aatacaacgt aatcttccga acacttatgg tgaggacacg aaccacgtta
       61 tatgaatatt atattttgca agacaagaga atcttataaa aagcagctgc acttttttcc
      121 caagaaaaca gctgtgcgcg gtcaattcct tttttagata attcttctcg aagcgtttta
      181 ttcaaagcaa gactttgcag tgcatctctt attgctactg tatttgttgg atcgaccaaa
      241 tatgctgcat cgccggcaat ttctttacaa gaagttgttt tcgaagtaat cacgggcgtg
      301 ccacatgcca tcgcttcgac tattggcaag ccaaagcctt catgaagtga gacaaaaacc
      361 aagcctaatg cttgttgata aagttcaatt attttttgtt cgtcgattgc ataatttata
      421 atttccaaac cttttatttg ctttactgta tgtggtactt catacggttt taccaaagca
      481 actaatcgtg gtcggcttgc ctcatctaat agttgatagg cttcgagaag tcgttcaaaa
      541 tttttcttct tttgataaga gatgattgtt aaaaaataag gtgaagtcgt ttcaattttt
      601 tgcgctggtt taaacttatg gtggtcgata ccattccaaa ctgctgttat ttttgctgtc
      661 ggaaaatcga tagtttgtat aagctcactt ttggtgtatt cgctcaccgt catgatgtgt
      721 gcaatatgtt tttgcatcaa tgtccatttc aatcgttctt tccagcattt tatttgtcca
      781 cttatttttt gctgccacga aaaataaaat gtatctattg gcaaagtaaa catagccatt
      841 ccgtgtacgg taagtattaa tggacgcgct aagcttctga attttaaggc tggcgcaatg
      901 cctccgccac aacgcggatc ccaataaata tcagcatctg tagttgctac atcttctaat
      961 gtagttattg ttacaaaatg tactttttct ttttcttgca atatgctcat tatattccat
     1021 gcgcagtgtt tcatgctata gggcgtagtt tccgggagat atactgctac tttcatgcta
     1081 cttattcagg ttgataaatg gaatataaac gatataaaat ccaaaatgtt tcttgatata
     1141 aaatgttgcg acaatattcc ataatgccat acttacacta tttgcgatag ctgcgccaag
     1201 aatgccgaat tgtggaataa gcaataaatt ggccaatata tttatagcca ttccaataaa
     1261 agagatattt ctaaactgac gatgatgacc agtcgcgttc ataagttgtg ccacaggacc
     1321 acatactgta tttactatct gtcctaaagt aagtacgctt aagcacattg cacctttcat
     1381 aaattcatca ccatacaagc acattacata tttagagaaa aataacaaaa caataaaaac
     1441 gggaagactc aataaaaaaa tgagctttga tgattttact gaaatacgtc tgacttcgtc
     1501 ataattttgt tgtgtatata attgcgtcat tttcggctgc actgtagtat ttaatgcgac
     1561 tagcaaagta gcggccaaca tagaaatatt tgtacatgtt ttataaatac caacggcatc
     1621 ggtgccgata tcatttgctt ttaaaataaa aacatctgca ctattattta ctgcaaatgc
     1681 taaataagtc atcaacatcg gaaaagctac agttataatt tgctgatgtg acgtttcaga
     1741 aactgtatca atagcggcgc ttggtttatg aaaaaattga taaactggaa ttaaagaaaa
     1801 caaccaaggc aaaacgcata gcgtactcca atgtaagtaa atcggattat aggtattgaa
     1861 aaaataaaat gttatcagca agagcacagc tattcgaata attgtgggca ctactaatga
     1921 catatttgct gtgtgtactt ttaaagcagc gcgcaagctg aaataattta attcacctaa
     1981 aacaaaaaat ggaataaata aactagagat tcgaaataaa ttcaccatat ctggctcatt
     2041 ccaatagttg gcaagccaag gtgagataaa atataaaatg atagctgaaa taaaagacaa
     2101 tagcaaaata atttgattgc ctttaagaaa aacagcatat gccgttttta aattatactt
     2161 ttctcttagc tccggaatta gtttttgtaa tgcctctttc acaccaaaat tgacaaaaat
     2221 ggcaaatatg cccattaccg ttaccaccag cgtcaatttt ccagatgcat cagcgccata
     2281 gaatcggccg atgagcacgc ctgaaacaaa acgcaagcct tgcacaaaaa acaaagtaat
     2341 cgcagtagaa aggctgcctt tcagcacaaa actgaagttt ttatcacgca atgcgctttg
     2401 aaatttttct ttataattgc taaatgccaa tgctatcgtt tacgcacaaa aatagtcatt
     2461 gtattttatg aatagtacat ggtttatttt tattattttt gaaaacagaa attgtatcgc
     2521 taaaaccaag gcatgttatt caactcttta cattttttac tttttttccc aatattctgt
     2581 tatctctatt tttctgtagc taagaaaaat cagtggaaaa tattagttgc tggcggatat
     2641 tatttctatg gtaattttag tccatttttt gccattatct tatttgttat tacctgtaat
     2701 gattttttag caggaaagat gatggacaaa gcaagtgaac aatccaagaa aaaaaagtgg
     2761 ttgtttctga gtatggctgg caatttaggt attcttttta cttttaagta tttagatttt
     2821 gcggacaata acattcgcga attgcttgcg gtatttaata ttccgtggtt tgtacctgag
     2881 ccttatttta attcatggat agtgccagtt ggcgtgtctt tccttacttt tcagtccttg
     2941 agttacacga tagatgtata taaaggcgtc acaaaagccg agcaacactt tggatatttt
     3001 gctggcttta ctgctttttg gccagtgatg gtgaatggtc caattgagcg cgccaaaaat
     3061 ttattaccac aaataaaaga agaacatcga ttcgattatg acagaatgcg tcatggctta
     3121 tttcgtatgg cttggggatt ttttaaaaaa gctgttattg cagataggct cgcttatttt
     3181 gtggacaata tgtatttgca ttatcatgaa atttcaggct ggacaactat tatcggtggg
     3241 ttcttctttt tcatgcaagt ttattgcgat ttttcaggat attccgatat cgctttaggt
     3301 gctgcaaaag tgataggcat tgatgtgatg gaaaatttta aaagaccttt gtttgccaag
     3361 aatttagcag acatctggac gcgctggcat atttcacttt ccacgtggct aacagactat
     3421 gtcttctttt atttaggcgc atacaaagca agtggtgcta aggttatttt caatgtcatt
     3481 tttgtattag ccatttgtgg actatggcat ggtgccaact ggcctatggt gttagcattt
     3541 acaatggttg gcgtatttat ggctattcga tattggtggc aggcaaatgt tgttcgggct
     3601 atcaaaccta gtaatagcta tcgcctgttt gataagtatt ttccaaattg gctacacagc
     3661 gttattactg tagctttatt cgttttttgt tttctattat ttagagtaca caccgcacaa
     3721 ttagcctaca acagctcgca tccgaatgca cctgtagaat gggcaagcat ggcaaaaact
     3781 atatattata atcttttcaa aatacataca gcaggctatt tccatggctg gattacacat
     3841 caaggtatcg tgcagttttg tgtagtcgta ttttttatgc ttattttaat cagcacagaa
     3901 gcactcatcg gcgacagaaa aatcgaaact gtagtgcttg caaaaaacaa acaacaacgt
     3961 tggacaattt atattttatt gctgataagt attgtatggt ttggcatttt taataacacc
     4021 tcatttgttt attttcaata ttaagcaatg ttcaaaaaaa caattataca tatcgccatt
     4081 atcgtactta tattattcat aagcgctgca atcgtgcgct tttttatgcc gttttactgg
     4141 ggcgacgcca cgcaaactgt gaagtatgaa tattatgaaa aaaatgcaga aaaatataat
     4201 gctgtttatt taggtggtag tctcgaatat cgacacatcg acccttcaat tatagatagt
     4261 gttgcacaaa aaaatggaat tgattttcat tcttttaatt taggaattga tggccatggc
     4321 atcatacaac aactcaatga tttagagggt ttgctaaaaa tcagaaatcc aaatttaaag
     4381 tacgtattct taagcattag cagtgaatct tatttcttta aagcaaactt acatacacct
     4441 aaatggatat cttggcacac tgcaacgtca acttataatg ccttaaacct tacatcaacc
     4501 ttagatgatc ctttaaaatt tagaactaag tttagcggat tttggttgag tagctggata
     4561 gaaaacttat ttaatatcgg cactgttcct gatatgctta aatacaaatt tgataaatct
     4621 tatttagaca ctagttatgt tggcaaaaat aaagatggtt tttatcctta tgattatgaa
     4681 gagagccatt tgtttatgga atataaatgg gaagacacct tgcttttaga atcgagacat
     4741 cggtttgaaa aaagcgaccc atacacagat actttaaata aaagtgtgct taactctttt
     4801 actaattata aaacaactga tgctccaaac aaagtaatga cagccttata cttagatgcg
     4861 tataagaaat gtaagaagaa aggaatacaa gtgttttttg tgttgcctcc aaaagcacga
     4921 acttcctaca gcttgctatt gcctgtgttt aacgccatgc ctgaaggaag caaaattgag
     4981 ctggcagaca ttagaaaata tccaaaattt tattcgatag aatatggtta taatttccat
     5041 catctcaact acaaaggcgc tcgattatgt agccaagaat tggcgaaaca attgattcca
     5101 ttattaaaaa gcgaggaaaa taactaaacc atctttggtg ctttatgcac tttaaatgca
     5161 aaatatctct gaagaaaata ggacgttgta ataacaattg ctgcggtcaa cattctcgcc
     5221 ataaaaaggc gaaactgaaa tacttcgaca aataatttta agaatccagt attcattgca
     5281 acacataatg ccactactgt taagtagttt atcatttgat ttctgccacg cactaaagaa
     5341 tttgtccata ctacatatcg acttaaaaaa aatcctgtgc ccgtgctaat cataaatgaa
     5401 aaaaacaagg ctgcaatatg cggactgaaa gtaaaaaatg gtgttacaaa attccgttcg
     5461 tgaaaaataa aatgatagga gataaaaaat aaagacatat caaaaacaag atttatgccg
     5521 ccacatgcca tatataaata gctttgctct ggaataaaca taaaaaacca tcgtctgatg
     5581 gtcaataaaa tatctcgtat tattttggca attgctcgta acactttcaa caaatataca
     5641 atttttgtat gatatgctta tgaggaaagc aagtctgcat agtgcaaatt tgtgattttt
     5701 aacgaaaatg gcatattgct gcatacaaat gcgctatact gttctactat ttgtaatttt
     5761 ttgtagtgga ataagacatc atcacagaca atttgatgtt cgtctgttgc cgtgtattgt
     5821 ttgtgtgttt ttgataatat ttcaacacct ctgccatcac atttaatagc gctttctttt
     5881 aatgaccaca tatcgaaaaa acttaggttc tggttttttg ctgtctttat ctcattccat
     5941 tcttcatcat taaaatattt tttaaataat tcaatattga tgtttcgatg tttttcaata
     6001 tcaatgccca ctttggcatc ttgtgctatt gcacaaatca caaattcgcc ggaatgggaa
     6061 atattaaagt caagagcatc attaagcatt ggcctgtctt tcctaccaat cactacgtca
     6121 tgcaatgaat attggacttg taaattttga aatgcatgtt tgagcaataa tttcccaagc
     6181 aaagatgctt gtgcgctttc catgtgtttg taggcattta tttcattttg aaaaatttga
     6241 ggcaacccaa ctaattgtcg ttgaaaaaca gcatcaatta attcctgagt gtgctgtata
     6301 tagaatatgt caatcttgtt attcatttca tttatacagc atttaaaaaa tgatttatct
     6361 actaacaaca ttcattttca tctttatata agtggctaca ttttcatttt catacaaaaa
     6421 aaaatgttta ccttcaaatg atttgatgtc tatattattt acaaaaaact tacgccaacc
     6481 ttgcattttt tcattcgata catttcgtac atcttgatct ccgaataata tagacgctgg
     6541 aacatcaata gcagcaatac tttcaaacga ttcattaaac tgctgcaaca gttcaaaatc
     6601 tgttcgtaat ataggctcat agaattccat caactcttta tgctgcaaaa gttgtgcggg
     6661 aacgccatca tactgctgca cgactttcca aaaatctgtt gaagaaatac ctacaatatt
     6721 aggttcttgt ttataagcat caggacttaa tcgcgatgac aaaaataaat gctttggcat
     6781 ttgtttattt tcttgttgta atttatgtgc caataaatat gcgattgcag cacccatact
     6841 attgccaata atacagtatt cattaggcat ttttcttatc aattgctcat aaatatcatc
     6901 aactgcttgg taaatatttt taataaacgg ctcactgatt ctgtcactcc ttccgggcaa
     6961 ctcgaatgcg atgctttcaa tttctggtaa taaattttgc tgtaaattac gaaatgcata
     7021 tttgtcaccg ccagcatgat gcaagagtat cgctttcatc tactatttag ttttttacag
     7081 ttggcaattt cttatcaatt tttttgaacc agcgcatcaa tgcattatct gttgcttgaa
     7141 tttcatgcca atcaccttgg aaagaatgtt ccatttcttg gttaaacata atatctgcca
     7201 ttgtgatggc ttgtgtagta tctacagtgc ctccaccata ataagtatca atcaaatatt
     7261 gatgcaaatc ttttggcgta ttgtattggt gaatgatttc cattttcaat tctgcaccta
     7321 tatgcttttc cattaacgac aaatactcac cactcatcat gctatcaaat ccttgctcgc
     7381 gataaggttt attgatgtcc actttttgtc cagcctctaa gcctaaaact tttgcagctt
     7441 caatttgcat atatgccaac aaggtttctc gtgttgcaat tgcttgcgtg ttgcttgatg
     7501 cagtttgata atttgaatcg tgtatttcaa ttttattatt gcttgtatgc gtattcgatg
     7561 agtgtgcaaa gtgtatcgct tgtcttaccg gattttccca atatactttt tgattccacg
     7621 catacgatgg caaggcaact ctatttattt ttttatgtag atagaatata gaccaattta
     7681 tttttatatc agcacaatat aattgttgaa gtgttttatg aaaataagtg atatcgtttt
     7741 cctctcgctt ggcagaaggc aaccacagcg cgtttgagtt cgtatttgat tgtttcgcca
     7801 agctaattaa cacaggattt ggtcctactt ctatatatac ttcgtaatta tttttttgaa
     7861 gataatcgac accttttgca aactgaatgg catgtctaat atgatttgca aagtaagcag
     7921 cagacaagtc tgctttcgtt atttctttgc ctgtcaaatt ggaaactaca gtaattgctg
     7981 gctgtttgaa agaaaattgc tgtgcaaata ttgtaaattt ttctacaatc ggctccatca
     8041 aataagaatg aaatgcgtgc gacacttgca aaggaactgc ttttattttg tgctcttgca
     8101 gcattgcaac aaaagtagca acatcttctt ttttaccaga tatggtacaa ttttttattg
     8161 aatttactgc ggctaaattt acatttgaat tttctagaaa aggttgcaat gtttcttcat
     8221 tacaaaacac ggacgccatg cttccttcat gcataggtag tgaattcatc aaggctgcgc
     8281 gtgcaataac taattgcact gcatctttca aatcaatagc atttgcgata gtcaatgcga
     8341 caatttctcc tatgctatgc cctattaatg cagatgcgtg cacaccgaag ctcatccaca
     8401 attttgcgct agcatattcc actacaaaca atgctgcttg tgtgtatttt gtttgatgaa
     8461 ttaggttttt gtctatgctg tcattccaca aaatatcttt aatcgaaatt tgcagagctg
     8521 acttcatcat ctcatcacat tcatcaatag tttgcttgaa tatatcaaag gtgtcataat
     8581 aacttttaca cataccaaaa tactgtgcgc cttgacctgt aaacataaaa acgacttcct
     8641 tatttctatt caagcttggt tgtgagacag catatttttc cgattttata tcttgtacaa
     8701 aatccgtcaa accctcaatg attgcttgtt tattgttagc tacaatgctt aatttataat
     8761 caaaattatt tctatgattt tgcaatgtaa aacagatttc tgttaatgaa ttttcttgcc
     8821 aatctgtctc cgacaaatat ttatttgcta aactttttaa agcatgtgca tctttagcgc
     8881 tcaacacaaa atgcaatggc gaaggcactt catcgctttt tgattttggt ggtgctgctt
     8941 caaaaatcat atgtacgttg gtgccagaat aaccagataa attaattgca gccagcattg
     9001 gctttgcaat atcgctttgc catttgatac tttccgtatt tacctttgct tggatcgtat
     9061 tccaatcgac gtcttcattt aaagctgcta tatttatttg cgctggtatt tgttgatgtt
     9121 ttaaaatttc gattacttta aaaatcatcg gcattccagt acctgcttct aaatggccta
     9181 tatttgcttt tactgaacct acataaatcg gctttgttct gccagtataa gcatcttgaa
     9241 tggcctgtat ttccaatgca tctgtaaaac gattgccaat gccatgtgct tccacatagt
     9301 cgatatcatt tacagaaagt tctgcatttt tcaatgcttc ttttatcgtt tcgatttgta
     9361 tttccggatt tggcgcagta aagccattgc ttctaccatt gtgttttacg gctgagcctt
     9421 taataattgc ctcaatgtta tcattgtcat ttgtagcatc ttgcaaacgc tttaacaaca
     9481 ccacaccaca accttcgcca cgtacataac cattggcatc attggcaaat gcattacatg
     9541 catcatttgg cgaaagcgaa cccaatttag acaagccgac aaatggctct ggtgtcaaca
     9601 ataaatttgc agaagcaaca attgctgtgt caatgatttt cttattcaaa tcttcatttg
     9661 ctaaatgcac ggctaataaa gaagaagcgc aagcagcatc taatgcaaaa ctagtgcctt
     9721 tcaaatcata taaataggaa actctgccac aagcagtacc gaaaggaatg cctgtagtat
     9781 catatacatc gacagtatct aaactgccac tgcgaaaacg cgcttgaata taatcagaat
     9841 tgccgagagc aatatacaca cctgtgttgc tgcctttcaa tgtatctgat gcaatgcctg
     9901 cattttcaat tgcttcatac acgacttcta atatcatgcg ttgtaacgga tcgatggact
     9961 ttgcttcacg cggtgatatg ccaaaaaaat ctgcatcgaa tgcacgtaca tcattatcca
    10021 aaaatgcacc aaactgcgca actgtttttc caggaatcga aacatctgca ctataaattg
    10081 catctacgtc aaatctatct ttcggaattt ttgaaaatac gcttcgttgt gctgataata
    10141 aattccataa tttttttgtc gaatcgatgc tacctgccaa gcgaacgcca gtgccaataa
    10201 tagcaatcgg ctcttttggt aatgctatat tttctttagc ttgtgctaat tctaattcca
    10261 gcttttttat ttttatcaga gacttttgta atatctcttg catttgttgt aattgttcgc
    10321 tcatagtgtt taacgtttat ctgaattaaa agcaaaatca tttaatcgac tttgctgctt
    10381 aattctttca tcaaatcttc taatgataaa tttttcactt gtgtttcaat atcatctagt
    10441 ccagcgctag aaggtgttga tgatattacg tctgaagatt cattggcaaa ttgctcattt
    10501 aattttagtt cattggcaat aaagtctgct aatttttgca cagttggata ttgccaaaca
    10561 acagcaacgt taaacgtcaa attaaaatca gcttgtagct tgttttttaa ttgcaaagca
    10621 tgcaaggaat cgatgcctaa tgctttaaat gtttcatctt cctttatttt agaaacaaga
    10681 agtttcgtcg ccgtagaaat atgttgtttg atgtgttctt taatgtgttt tttatattca
    10741 tcaatatttg caaacgagat actttccgct tgctgttcct cgtgtgtggc ctgtactact
    10801 ttactataaa aatgattgtg caacacggcg tgattaaaat tcgaccattt agaaaagtct
    10861 atttccattg ccatgatttg tgttgcatca cttaaaaata aagtatcgaa ataagcgtat
    10921 agctcatttg gttgaatagc ggtaacacct tgttcagcga gtctatctgc acgattttct
    10981 tggcgtgccg ccaatccgat agctgctatt gttccaaaat ctactgctaa tgcttcgagc
    11041 tgattggcgc tacggtaatg cgctaaacta tccataaaag tattggccgc attgtagtta
    11101 gcttgtccgg cagaaccaac aatgcctgct gcagacgaat acaaaacaaa gaaatctaaa
    11161 gattgatttt ttgtcaactc gtgcatattc caagctccta ttgctttggt agtaattact
    11221 gattcgaatt gagctttgga taaattttcg aacgcagcat cgtctaaaat tcctgctgca
    11281 taaaaaacac cagctactga aggtgctgaa gaaatggcat cttgcaagga agcaaaatta
    11341 cttacgtcag caataacatc attaatataa acaccttctg atttatattt ttcaaatatt
    11401 gattttgctt cttcttttgc accactgcga ctcaagacca aaatgttttt agcctgctgt
    11461 tgcactaacc aatttacagt atataaacct aagccgcttg tgccacccac cacgacatac
    11521 gttttatctt gtttgatgct ttttgagcgc ttaggcaatg ttgaaatttt ctctatgttt
    11581 gatgcatagc aagtctgttt tctgatggca atttctttat atacatcatc tacaaaaacc
    11641 aagctagcaa gttgcttact atctatatct tttaattcat tagcaatatc gatttgtaaa
    11701 aagtttattt cttcatgttc attctctaat gtacgcaaaa cacctgtcat catagatgaa
    11761 ttcaggtttg cagctgtatc attcagcaaa gtaaaagcac cgttcgttac aacgcaaatt
    11821 tttggacttt tgttgcttct attaaactgg tgtagcatct tctgtagtga taatatgcca
    11881 ttttctaaat tcaaaagaaa aggattgtca ttatacaaac tgcctacatg tatcacgata
    11941 tctacatcaa tattttcaaa atcatcttgt gcatcaatcg ttgtgactat gcacgcatgt
    12001 tgtcgcaaaa cattttctac agcatgtgct attccataat tatcttttac gattaatatt
    12061 ttcttagctt caatatttgg tgctacttgt atttcgtgcc aattgatatt atataaattt
    12121 ttggaaatat ctttttttac aattgaagta ttgatagaat attcaggttg ttggtcgaac
    12181 caataccttt ctttttgcca tgcataattg ggcaattgta caaatgcgcc aagatttgga
    12241 taaatatttt tccaatcaaa tggataatgg cttgcttcga gtgatgcaat attagaataa
    12301 aactctatta attcattttt atcgcgtgtt atggacgcta ctactatggc aggcttatcc
    12361 tgaatatttt cgttgatagc atgtgtcaac aaagcatgtg gactcatctc tataaatgct
    12421 acttgttctt gttggactat agcacgtata gcatttccaa attgtacagg attgcgcaaa
    12481 ttttttaccc aataatccgc ttttaattta tcgccttcta tgatttcatt gagtgcagtt
    12541 gagtaaaaat gaatcgttga attttgtggc tgaatcgttt gtaatgcatc aaataaatct
    12601 tgttttattt cgtccatttg tgttgaatgc gatgctacat ttacctttac ttttctatta
    12661 aaccttcctt gtgcttccaa ttttgcaaat acttcatcta atgctgctgg atcaccagaa
    12721 ataacggtag aatttttact attcattaca gcaacggaca gtctgttctc ataagctttt
    12781 aatatttcat tcgcttcttc tgctgtcaaa tctgttgcac ccatttcacc ttttccgctg
    12841 agttgtttca tcagcttact gcgtgtgcaa ataattttag cagcatcatg caatgtcaac
    12901 ataccagcaa catacgctgc cgcaacttcg cccatgctat gtccgacaac actatcagga
    12961 aaaacacctt tgctcatcca cagatttgcc aatgcaattt ccattgccac taaaacaggc
    13021 tgtacaatat ctatttctga caatctacta gaagcttccg ctttattaat ttcatcaatt
    13081 atattccaat caacaaaata agagaatact tgtgcacact cttctaaagc acttttgaat
    13141 acaggttcat gttgcattag catttttccc attgtaatcc attgtgcacc ttgtcctgga
    13201 aagataaaaa cggtttttat tttttctttt gtgtcaaatt ttttcttgtt ttcataattg
    13261 ccattttcag cgtaatcatt taatttttca attaattctg ctttagtagt ggccacaaat
    13321 gtttcgcgca aatcaaagtg tgcacgacgc aagctggtca tcgcacaaat gtcttccaat
    13381 ttatattcgc tttcttgaat aaaagtggcg tatagtttgg ctaaatcaaa taatgcgtgt
    13441 tcacttttag cagacaatgg caacacataa acatcatggc gtaattcatc tttctgcgtt
    13501 gtatttatct tcttgggtgc atcgccaatt atgatgtgag cattagagcc agtagcacca
    13561 aaaccactca cgcctgcata tcgaatttta tcattttcac gcttccattc tatattatct
    13621 tttgctactt taattggaat attttcccaa tcaatcaaag tatttggcgt gtggaaatga
    13681 atagactttg gaattatatt atgctgcaaa cctaagacta ctttaaacat actcgccatg
    13741 ccggcattgc attccatgtg tccgatattg gactttattg cgccaataat taatggtttt
    13801 tcttttgtgc gaaatttttc ataagccagc attaaaccac gcacttcaac tggatcgcct
    13861 acttttgttc cagtgccatg agcttcaatg taatcgatgt cagtaggttg aagttgcgca
    13921 tttttgagtg ctgctgtatg cattaagcct tgtgctgctg tgcttggcgc agtaagtcgt
    13981 ttgctcttgc catcttggtt tatggcagag ccttttatga tggctaaaat attatcatcg
    14041 tcattttgag catcacttaa tttttttatt atcatagcac acacgccttc cgagcgaatg
    14101 tagccattgg cggcatcgtc aaatgatttg caacgtgctt ctggtgagag tgcacctaag
    14161 cggcagaaat ttaaagattg gtctggctct gatataatac ttgctgctcc aacgacggca
    14221 attttacaat ctttgtttcg caatgcttgt attgcgacat gcgtacaagt cagcgctgac
    14281 gagcacgctg tatcgatagt catgcacgga ccacgcaagc ccatggtata ggaaattcta
    14341 ccagatgcgc ctgttggccc tgtgcctgta taacaatatc ctgaaataag tttatagtct
    14401 ttagatcgga tttgtttggc ttcataatct acattatcta taccaataaa tacgccagta
    14461 tccgagccga caagtttatt gacgtcaata cctgcatttt cgaacgcttc atgtgtaact
    14521 tctaataaga tacgttgttg cggatcggtg ctttctaatt cgattggaga aatattaaaa
    14581 aatgcgccat ctaaatattc tatatcttcg ataaatccgc cttctttcac atagagttta
    14641 cctggtgcat ttgggtcggc atcgaataac gcatctttat cccagcgatt tgcaggaatt
    14701 tcttctatgc agtctatacc attaacgagt actctccaaa ggtctttagc attttttatt
    14761 ttccctggca agcgcatagc catgcctata atagcaattg gctcattgct agcatttttt
    14821 tcattttcta atgccgattg taatacagat atctgttgtc ttagttcaga aagttgttgc
    14881 tgatatgcgt caatattttc cattgtttat tatatagcag aaaagataaa gttatgcaat
    14941 ataaccttat caaacaataa acaacataaa aactaagtag ttgttgcagg cgtttcttgt
    15001 gcagccaatt ttgctgcttt tactttatct tcctctctgc catcattaat tgctttttgc
    15061 ataattaatg gcaaaatgat taacagaata cttactgcaa agccaacggc agcaaacaag
    15121 agtttacttt tacgttttgg tgctgttgag aacattggat catcaattac acgaacaatg
    15181 ttgctagcag gtgtaagacc cacttttgca ttttcttttg aacttgatgc gtcattgtac
    15241 aatgcaccta atacggcaag ttcttgcatt attttttgtt ggtcgaccgt ttcaattcgt
    15301 tttctgttaa aaatattttg atcttgcgat tgcgcaatta atcgttgtct atatgctatt
    15361 tctccagaaa tggaatcgag tcttttgctt gtgcttttaa atgttgtggt tgcattttcg
    15421 agctgtcttg cttgataaaa ttcagtagtt tttttcaata aaacagaacc taatgttcta
    15481 gaaacttcat agtctggtgt ttctaattcc gcacgaataa cgcctgacat tacatcgtat
    15541 tcagcagtaa gcaaaccatc aaaataatcg tcatacatca taccgaagaa atccatttct
    15601 gttttagaga tttcttcaac agagtttgcg ctgaatttaa aatctttcag cactgaatct
    15661 ttttcaaatc cgtcgtcata ttttaatgca tggaaataaa aatttgccat tttatctttc
    15721 tttccgttga tttcaacgtc ttcaatcata gaattaagaa agatatttct agaagtaaat
    15781 atacctgtca acacatcatt agaagaacca cctgcaaacg aaacgcccat catagacatt
    15841 aagccaccca tagcgcttgc ccgtgagtcc aacgcattaa aagaaatgtt tgccacatat
    15901 tgcggtgcag acagcttagc aaaataataa ccaccagctg ttaatgccac gacaccaact
    15961 atagaaatgt accatttttt taatagatat attaggtagc ttctgaatga ctccagaaca
    16021 tcgaccagtc taatatcttc ctctacaaat tgttgttgca tgtgcttatt attttttcaa
    16081 gacaaatacc aatgtgattg ttgatgcgat ggaagtaagt gcggcaataa cacttggcaa
    16141 tacgatattc caatttaatg ccgattcagg accacgaggt ggttttggtt ttttcacttt
    16201 catgtctacc gtgattaacg caccttcatt atctactgtt ggataatttt ttattccaat
    16261 aaatgagcgt gtcatatcta cctttcggtt tggataaatc accattgttg acttcttcaa
    16321 tgctcctttt ttaaagccgc ctgcataatt tttaatatac caacgtgcgc tttttcctgg
    16381 cacaaatttt ccactaatgg taagtgtgct atctaatcca ggataacgaa ttgctccttt
    16441 gatgctcacc atttgattta cagttggaat ctcaatgatg tcaccacctt ttaatattaa
    16501 gttcgcacgt gatgcggtgt cagcaaaagc gtcttttaaa tgaaatatag attgatttaa
    16561 tgaattgtct ggccttgtta atcttgcatt tttaataaaa gcataaggtg ttaaaccacc
    16621 agctcgttca atgacgtcta atactttttc atttttatct aaaattggat acacacctgg
    16681 atacttcact tcgcctttta atgtgacgac taattgatca tcaaagccat aagttttgcg
    16741 aacaaaaact ctatccatag gtgataaaac ataagctttt gatgcttcat caatttctaa
    16801 attaacgcct acggttacgg tttgtacgac agtacgctga ggtacaaaaa tttgttctgc
    16861 tgaagagtca atatttatta cacgagagat ttcgatttta gaattggctg cttctttttt
    16921 taaaccacct gcataaaata gtaggtcgtt taatgtgatg ccttctgtat attctaatat
    16981 agtaggttta atgacagagc catcgatgcc gacgaaaaat ctttcaataa actcgtcttt
    17041 tgaaaacagt tgaatttggt cgaagctttg caacaatacg ttatcaggcg agttctcgtc
    17101 tattaaaata tttttcaaag aaaatttctt attgacataa gtattgtcgt ctaattttct
    17161 tttaatgtat gctctctcta aatacgtttc taagcgaata ccacctgcta tttttatgac
    17221 atctgaaatt cgatagccag cttttaattc atattttcca gcataacgca cagaacctac
    17281 aatactcacc gtattttcta cggtgtttgg aatagtggaa atagtaacga catcaccatc
    17341 ttttaattca aagtttgagt taacaagctg gtcgtagtcg atatcaataa tttctgcttt
    17401 atcgttgtta atccttgaaa cttgtatcga tttcgtatat gcatttgatg acaaacctcc
    17461 tgcaaatttg attaagtcat taagactttc acctttcagc aattcatatt ttgctggtcg
    17521 gcgaatggca cctgctattt ttacaagttt gctttgtgta gatacataaa tgaaatctcc
    17581 tttttgtaaa aagaaattct cttgtaataa tggattaaac aaaaactcat aaacatcaaa
    17641 tcgtttgatt gttttgccat ctcttcttac ttgaatgtcg cgaacgctac ctatttccgt
    17701 aatgccattt gccgcattta tcgcattata cacagagttg attgctggaa tttgatacgt
    17761 tccaggcata tccacttcac ctacaatatt cacatctatt gtgcgagagt agttgagtgt
    17821 tatttcatat tttgtatttg atggatttat aaatgtagcc aaacgttttc caatttgtgc
    17881 ttttacagcg ccaaacgtta atccttttac ataaattcta ccaaattcag gaatttggat
    17941 aaatccatct tcatttactt tcaatttatt attatagtca gcataacccc aaactgcaat
    18001 attgatttca tcatttacgt ctaaaatata agaatctggg gctttcgaat ctgcagattt
    18061 agaaaacaat gccaattttc cacttccaaa aaaatcatgt ccaaatattt catttggttt
    18121 tacatcttgc gtcatcgctt tcgctttctc catgcgttcc atcatcactt gcatagaatc
    18181 ttggcggatt ctcatttgca ataaatcttg caatacgtct tgcatttgac cttgtgaacc
    18241 tgcattatct gcttcggact tttgttttag atatttttga atagtgcttg gatccaatcc
    18301 taatagttgc aattgtgaca aatttggatt tgacgtggct gatgtactcg ttaaagtctg
    18361 caattgttta gctgcatttg ccgctgcagt agtaatagac gtaccagtca ttgcgccagt
    18421 tagatttcca agattttgag caaaacttgt atatatcatg cacaatagaa gacacagcga
    18481 aactaatttt ttcattatgg tgggttgttg aacggacaaa ggtaataaac tatcggaaat
    18541 ttatatagtg aaatgtaaat taagcgatta ggtctttcat ttattaagat atattaagaa
    18601 aattgaaaaa accgctcgat ttttgcttta gtcaatgcca aaataaattc aaaataagcg
    18661 taattttaca tccattttcc ttttgttaaa tacattatta cgtttgaaat atgacttatc
    18721 atactgacac aatagttgcg ctctcgacgc cgccaggctt aggcgctgta ggtgtaattc
    18781 gtatttcggg cacacgagct attgagattg ttaatgctgt ttttaaaggt aaaaatttat
    18841 tacaacaggc atcacacact ttgcatttcg gtaaaatagt gcacgagcag caaattatcg
    18901 atgaggtgat agtcggtata tttttagcgc caacatccta tactaaagaa aacattgctg
    18961 aaatatcttg ccatggctct ccttacattt tacaaaaagt aattgaagta ctgataaaaa
    19021 atggcgcacg ttctgccaag cctggcgaat ttacgatgcg tgctttttta aatggaagat
    19081 tcgacctttc gcaagcagaa gctgtggcag acttgattgc agctgacagc gaaattgcac
    19141 acaaaaccgc tatgcagcag atgagaggcg gattttcatt agagataaaa agactaagag
    19201 aagagcttat tcattttgct tcattgatag aattagagct cgattttggg gaagaagatg
    19261 tagagtttgc cgaccgcatg gctttaagtg ctacaattga acaattgctc cgcacaatct
    19321 acgcactttc cgcatctttt aaggttggaa atgtcattaa aaaaggtgta ccagtagtat
    19381 tagcaggtcg gccgaatgct ggcaaatcca cattgctcaa tgcgttggtc aatgaggaaa
    19441 gagccatagt aagcaatatc gcaggaacga cgcgcgacat cattgaagtg cctataaata
    19501 ttgatggtct tacttttaga ttaatcgaca cagcaggcat tcgcgaagca tcggatacca
    19561 ttgaggcaat tggtgtgaaa aaaactttcg aacaaatcga aaatgcagct atattactat
    19621 atatattcga tgctaatgac ttgtctgaat tagaagtttt agaagatatc aataaactaa
    19681 acaatagcaa acttaccatt gtattagtag caaataaatg cgatttgtgt caggaacata
    19741 cattgggtac acttgaaaac tcaatcaaca aattgacgaa aaatgccttt cctatttttg
    19801 aaattagtgc acataaacaa caaaatatac atcaattaaa agcatactta actagcgcat
    19861 taggcattaa ttcttactta aatgacaaca cattagttac taatattcgt cattttgaag
    19921 cattgaataa ttgctattta tcactggaaa aagtaagcct tgcactacaa aataaaataa
    19981 cgacagattt cttagcgcaa gatattaggc atgcactttc agcactttca gagataacag
    20041 gcagcatcac aacagaagat ctattagaca atatcttctc aaaattttgt atcggaaagt
    20101 aacatttctt aagtattggc taagctaaaa tgctaataaa tgcaatatag ttgcaataaa
    20161 aacttcacat tttcgttaca atacatagaa ataaagaaaa aaatagcata tttttacgac
    20221 cttattaaat taaaattaaa atcaatatca ttttaaactt aaattgacaa acatggctca
    20281 agcacaaaca aaaaagaatt ccggtggatt aggtgccggt ttagtagttc ttattgcaat
    20341 catcatttgc aatattttgt tctacaccgt ctttaaatct caactcgatg agaaaggaca
    20401 cgaaaaaaat attattggtt tattctataa aggtggtgtt atcgtacctt tcttgatggc
    20461 aacatttatt acttgtatta ctttcgcaat tgagcgtttg ttaactattt ctaaagcagc
    20521 aggaactggc tctttagaca catttttaca aaacataaaa tctaaattag taagtaacga
    20581 tatcaatggc gcacttgctg aatgcagtaa acaaaaaggt gcagtagcaa acgttgttca
    20641 agctggctta cataaatatg cggctatgga aaaagacaca gagctaaatg gcgagcaaaa
    20701 aatcatgaac attcaaaaag aagtagaaga aactactgct ttagaattac cagcattaga
    20761 aaaaaatatg tggattttat ctactatcgc atcagtagga acattaatcg cattattagg
    20821 aacggtaatc ggtatgataa aagcgttcgc agcattagcg gtaaacggta atccagatcc
    20881 agctgcttta tcatcaggta tttccgaagc tttagtaaat acggcattag gtatctttac
    20941 ttctgcatta gcgattatct tctataacct ttgtacacaa aaaatcgata aattaacata
    21001 cggcatcgac gaaatgggtt acagcatttc tcaaacattt gctagtcgta aacactaatt
    21061 catcactcgg taaaagatta aaatgccaaa atttaaagta aaacgtggaa gcacttgggt
    21121 ggacatgaca gccatgtgtg acgtctcgtt cttattatta acgtttttta ttttaacggc
    21181 taaattccga ccagcagaaa ttgttcccat tacaacgcct acatcacgtg ctgaaaacgc
    21241 agtagcggaa gatatggtaa cgttctccat cgacaaagat ggaaaagcat atatgtctat
    21301 cggaaagcca gaaagaaaaa ctcaaattat tgaaaaattg gtttctcttt cacaaaataa
    21361 atacggaaat atcgacgaga acacacaaaa attatttact cgtttggagt tagtcggtgt
    21421 acctatccaa caattaccac aagctgtaaa aagaagcact gctgaactta cagaaatgcg
    21481 cgtagcaggc cagcttagtg gtattcctat ggatagtacg aacaaccaac ttggcgattg
    21541 ggtacaagct gttcgctatg tctatgcgga aaccgataaa ttatcgaatc caccagtagc
    21601 cataaaaggc gacagagcgt ctaacataaa aggaattaaa agattaattg aaatttttaa
    21661 agaaaaagac gtgtacaaat ataaactcgt tacttcttta gaaaataaag ttgaaaaata
    21721 aaataaaata agatggcaga tatagatacc tcgtccggtg gcggacacgg caaacataaa
    21781 ggcggtgtcc ggtccaaaaa attatctacg agagtcgatt taacaccaat ggtagatttg
    21841 gcgtttttat tgatatcttt cttcatgctt acaactcaat tgtcaaaatc tgtggctatg
    21901 gatttagcga tgcctaaaaa gccagacaat gtagaagtga aaccagaacc tgttgcaggc
    21961 tctaaggtat taaatctaat agcagataaa gacgatgtgc tttggtatta tccagatgtg
    22021 gttgtaggtg gcctaaagaa aacagagtac acgccaagcg gactacgtca agtcatatta
    22081 aaaagacaag ccgaagtaaa agcaaaatgg ggtttagata acgatggtga tagcaaaatg
    22141 gtagttttaa taaaactaac tgatgatgca aattataaaa acatggtcga tattttagat
    22201 gaaatggaac ttaccaaaac caaaatttat gcaattcaag atgtcaatga ccttgaatta
    22261 gaagccataa aaaatggtgg caatgcaaac atttctgaat aaaaacatcc taaataaaaa
    22321 taaatgagca ataacaataa ctttgactat aaaaatgctt cgtgggatga tataatcttt
    22381 gataatctta ataaagatta tggtgcttat gttattcgta aattagtaaa taaacataca
    22441 cttagaggct ttggtattgc attagccggt ttcttaatca ttattctctc attgcgtttc
    22501 aatatttttt cttttaaaaa taaaaaagaa aaaatcgttg atttagaaat gacagatgtg
    22561 cagctagaac aagctccgcc gccggttgaa gcaccaccac caccacctcc gccgccacca
    22621 ccaccacctg tgagacctac aattaagttt atcgaaatgg tggccgcaaa agacgaagag
    22681 gtgcctgagg aaagagaacc acctaaacaa gataacttaa caaaagacga tcaaaatgtt
    22741 ggtgagaaag accaaaaagg tagcctagat gctagtccat tagtggacaa aggcacagac
    22801 aatcctgcgc cagcaccaga accagaacaa ccaaaagaac cagaaatatt tgatcgcgcc
    22861 gaggtgatgc caacctatcc aggtggcgtg ccagaattaa tgaagttttt aatgaaaaac
    22921 attcgttatc cgtctttggc tcgtgaaaat ggattagaag gtaaggtaat tgtgaaattc
    22981 tacattgaca cagatggaac ggttaaagat ccaactgtat taaaagacaa tgtaggcggt
    23041 ggctgcggcg atgaagccat acgtgtagta aaagccatgc caaaatggac gccaggttca
    23101 caacgtggaa aagcggtaaa agtatattat actttacctg taacgtttaa actacaataa
    23161 ttttaacgaa cattatcaaa aaatcctcaa cttacgttga ggatttttta tttttacaat
    23221 atgaattcct ttctacaacc agcattttta ttacgctttg cgattgctat tgcctatatt
    23281 attttaggaa tagtgctcat cgtattaccg attgacatta aactactaaa caacacaaca
    23341 aaaccgctct tttcattgtt actcatagct tatggcacat ttcgattata tcgagctttc
    23401 caacttttaa aagaagaaga aaaataagat gcagtatttt acctactttt ctcggtttat
    23461 ttctatcatc attctgctat tgacaagtag ctgtaatact caaaataaga cagaacagca
    23521 acatattaat gaaagacaca aaggcaatgt cgtcattact gcagacatca atctacgtta
    23581 catattggaa cagcttgctt caatttatca catctattat cctgatgcca acatctcatt
    23641 caaatacaca acagaagacg atgctgttag caattttaaa ctgaaaaaaa acgacattat
    23701 tgcagttgaa aaactgctat ctccagacga aattgctaat gcacaattcc atcatcaagc
    23761 aaaggtatta gagaatatat ttgcgtatga tgcgattgcc attattagca ataaacaaaa
    23821 tagcgatagc atcttcattt tagacaaatt ggaaaactat ttgaatgata aaagcaaaaa
    23881 aatagtgttt gacaataagc aatcaggcat tgccaaacaa cttatgcaat acagtaatac
    23941 gagtgccgaa gcatttaaaa atgcctatgc tgtcaattcg gtaaatgaag tattgcaatt
    24001 tattgagcac aacaatgatg caattggttt tattccatat aattcattta gcaatagaaa
    24061 tgaaaaaaca gcacaacaga ttcgtactca attcaatatt ttaccaatat cctaccacga
    24121 tacgatgtac acactttcgc aagaaagcat agcaacgcaa aactatccat taattcggcc
    24181 tatagtattg tatattggca attgccctga attagtagca caaggatttg ccaatttcat
    24241 tttcggtaaa caaatctcta aagcactctt attaagtgga ttggtgccaa aaaacatacc
    24301 catcagagaa gtaatcgtca cagaagaatt tcatccaaat aaaaaataaa tcgtgtaatg
    24361 atatcttaac tttcattcat caatcttttt gtatttttat aggcacattt taattaaaga
    24421 tgatatgtca gaagataaag taaccattac gcttggcgga aaaacaactg aatgccctgt
    24481 tttagtaggc acagagaatg aatacgcaat aaacattgat aaattaagag aagatactgg
    24541 atttgttaca ttggattttg gatataagaa cacgggcgca acaacaagta agattacatt
    24601 tttagatggt gaagaaggga ttttgcgcta tcgtggatat gagattgccg atttagcaga
    24661 aaaatcaaat tttacagaag tagcctattt attgctatat ggcgaattgc cgacagcagc
    24721 acaacaagcc gaattcaatc aaaaattaaa agatcattct gacgtaccag ctgaagttgg
    24781 tgctatatta aaagcattgc caaaaggcac gcatcctatg gcacaattgg ccgctgtaac
    24841 agtagtgtta tctggtgttt atacagatgg tgcaaaatta agcgacgaaa atttcatcaa
    24901 tatcttagca aaatttcctg ttttggtggc gatggtcatt cgtaattcac agggcttaga
    24961 ttttattgct aatgataaaa gcttagacta tgtgtctaac tttttacata tgacatttgg
    25021 cactaaggca tcggcggttt tagtagaagc aatggataaa ttattgattt tgcacgctga
    25081 ccacgaacaa aattgttcta catcaacagt gcgcatggta ggttcttcaa atgctaatat
    25141 ctacgcatca atcagcgcag gtatcggcgc actttggggt ccacttcacg gtggtgccaa
    25201 tcaagcagtt ttagaacaat tagaagccat tgaaaaagac ggcggtgata cggctaaatt
    25261 tgtggctatg gcgaaagata aaacatctgg tttccgatta atgggcttcg gacatcgcgt
    25321 gtataaaaac tttgatccac gtgcccgtat catcaaaaaa tcttgtgatg aagtgttaaa
    25381 tgatttaggt gttcaagata aaaaattaac gattgctatg gagttagaac aagcagcgtt
    25441 aaacgacgac tattttgtat cacgcaaact atatccaaac gtggattttt attcaggcat
    25501 tatctataag gcaatgagtt tccctacaga tacatttact acgttatttg cactaggcag
    25561 attgccaggt tgggtatctc aatggaaaga attaaaagaa aacaaagagc cgattggtcg
    25621 tccacgtcaa atttatttag gcgcaactgt tcgcccttat acagacatta ataatcgtta
    25681 agcgataaaa ataaatagta aataaatgga cagccttatt gctgtccatt tttattgtaa
    25741 gttgaatgca agacatttta ataatccata tctttgcatt ttaaatcaat acattgcacg
    25801 aagtaaatcc aataatagaa gagcgcgctg tcttagtagg aatcattcga gataaccaaa
    25861 gcgctgagca agtagaagaa tacttagacg agttggcttt tttggcagaa acagcaggtg
    25921 ccattaccgt taaaacattt acgcaaaaac taaaacatcc tgatgtacgt tactttgttg
    25981 gaagcggaaa attagaagaa ataaaaacct ataaagaata tgccgacatc aatacggtga
    26041 ttattgatga tgaaatttcg cccgcacaac aacgcaattt agaatcagaa ttgaagtgta
    26101 aaatcttaga tagaacgagt ttaattttgg atatttttgc tatgcgcgca caaacggctc
    26161 aagcgagaac gcaagttgaa ttagcacaaa tgcaatactt attaccacgc ttaaaaggtt
    26221 tgtggacaca cttagaaaga caacgtggtg gtattggtat gcgtggacca ggagaaaaag
    26281 aaattgaaac agacagacgt gtgattaatg ataagatagc aaaactgaaa cacgaattag
    26341 aaattttaga caagcaagaa gcaacacgca gaaaaacacg cggcgagttg attagagttg
    26401 cattagtagg ctataccaat gttggcaaat ctacaatcat gaatttgatg agtaaaagtg
    26461 aagtgttggc agaaaataaa ttatttgcaa cgctcgatac aactgtgcga aaagtagtga
    26521 ttgaaaatat cgcattcttg ttaagcgaca ccgttggatt tattcgcaag ttgccacatc
    26581 atttagtcga gagttttaaa tctactttag atgaaaccaa agaatcagat attattgtac
    26641 atgtagtaga tgcatcgcac gaaaatttca gagatcatat taatgttgtc aatgaaacgc
    26701 tatcagattt gagtgtaact aaacagccac gtttagttgt ctttaataaa atggatttat
    26761 atcgtgaaaa atattttgat agatttctgc cagaagaaat aaagaaagaa ctatctgaag
    26821 attttgaaaa aaatatgcgt gcatggatga attgtaattg tgtttttatt tcggcaacga
    26881 ataaagaaaa tatagaagaa ctgcgcaaca ccatgatatt aatggtgcaa gatatgtacc
    26941 acaaacgcta tccatataaa agtagttttt atgggtaaag attgcttata tttttcttgt
    27001 gctatacaat aaacatcata catcttgttt aacatgcgct tcgtgatact tgttggtgaa
    27061 tttacctaat acaaaaatat cttttacaat taattctgct atttgcaaat tatcgatatc
    27121 atcttgcttt acttttctat attttggatg aagtgtgcca ccaggaccgc caggctgtgc
    27181 attcgtgtcg tattgaaatt cagaatcgac caaagtacca cgaggataca taataccagg
    27241 agcacctttg ccaatgatat ttaattctgt tggatgaggc aagcctaatg aatggccgta
    27301 ttcgtgtgca caagtcgtag aaccgctata taaattatct aacataaaat agccagtatt
    27361 actgcctatt gcgtcaacaa acgagatatt gagttggcta aattcttcta tacgtatata
    27421 attatttcta ggatttgtgt tttgcaaaat gctattagca cgtatccaat tgttatattg
    27481 aaatgtgata ttaaatcgca cagaaaacat attttcattc aatactactt ttccatttgc
    27541 ttcattccac atcgtagaaa tttcatcggc aatatgttga ctcaattcat aggtagcact
    27601 ttgtccataa caaataattg ccgatgaaat gataataact tttgcagatg aatctaattc
    27661 aattgtgccc attttaacaa ggtaaaaaaa ctatcgccaa atcaaaaata acaagctact
    27721 tatttctatc tttactgcat gcaatcgaaa attcaagata tattaaaagc ttctatttta
    27781 accaagcaac aggttttaga aaatgaaaat ttactaaaaa ccatgcaaac cgtaacggac
    27841 aaaattgtag attgttttaa acacgatggc aaactattgt tttgtggtaa tggtggttcg
    27901 gcagctgacg cacaacactt ggctgctgag ttttctggca gattctattc cgatagagag
    27961 ccattagatg cagaagcatt gcacgtgaac acatcgtatt taacagcagt tgctaatgat
    28021 tatagttaca acgaagtata tgcacgcatc gtaaaagcaa aaggcagaaa aggcgatatt
    28081 ttaattggct tatctacttc tggaaattct ggaaatatta ttgaagcttt taaagtcgcc
    28141 aaagaacgtg gtttaattat tattggcttt actggtgaaa caggtggaaa gatggcagca
    28201 tattgcgatt atttaatcaa tataccatcg acagacacac cgcgtattca agaatgtcat
    28261 atcacagttg gacacattat ttgccaatta gtagaagaaa aattttttga attataaatc
    28321 tgaaatctga aatctgcatt cataaatagt acaccagtta ttatactcgc aggcggcttt
    28381 ggcacgcgct tgagcacagt agtgaaagat gttcctaagc caatggcacc aattcaaggc
    28441 aaaccattct tgcattatat ttttaaagaa ctacaacatc agaatataga aaatgtagtg
    28501 ttgtctgttg gctatttgaa agagctaatc caagattatt ttcaagataa ctatttagga
    28561 ataaatattc aatatgcaat agaacatgaa ccattaggca ctggcggtgg catcaaaaat
    28621 gcattttctt ttgtggaaga tgatgctttt gtgttgaatg gagacacgta ttttgatatt
    28681 gaattatcaa aaataaaaaa tcaagaatgt gatattgttt tagcgttaaa accaatgttt
    28741 gaatttgata gatatggcac tgttgagctc aatgatgaaa acaaaattat ttcatttaat
    28801 gaaaaaaaat attgcgaaca tggtttaatt aatggtggcg tttattattt ccacaaaagc
    28861 ttatttgata aaatagattg cgatgaaaaa ttctcttttg agaaagatgt cttagaaaag
    28921 catttgaatg atttaaatat tcaaggaaaa atttttgata attattttat tgatataggc
    28981 ataccagaag attataacaa agcgcaaata gattttagat aaaaaaacgt aaaacaaaat
    29041 aatattattt atgaaataca ttttattgat tttcttgtta ttcatttctg tttctgcatt
    29101 ttgcaatgat ggactgagtt gggacgacat tactaaagaa gaagatggca ttgttactga
    29161 agttgacgga aatactatat ctggaatcat caaaataaaa ccatgcgcag agaaagtaat
    29221 catttctacc aaagattcta taataaagat taatatagtt ggaattaaaa acattcaaaa
    29281 aggtaatcat catatttcaa tgttgtattt taatgactct gttgattttt tctttggcca
    29341 cccaatagat acaatacata cacttaaatt ttataaagga tataattgtg atatttatgc
    29401 cggtggtggc ggatggaacg gagcaatggt aactggtatg agcatcaata ctcgattttc
    29461 tgaatcgtat attgtgagtt tagatgggaa agacggtgtg gattttaaaa gatctgtaaa
    29521 tggtcttaaa tttttagaaa ttgataatta taaaagaatt ctaaagaaga ctttcataga
    29581 taatcctaaa attatttctt atatagatac ttataaaaaa gtacattttg atgatattcc
    29641 aaaagtggta gaatacatta attccattta ttaattatat gccaattaat atagataata
    29701 catggacttt atttctcgat cgcgacggcg ttattaataa aaatattgat ggtagttatg
    29761 ttttagattg ggaacaattt gaatttttag atggcgtatt agaaacgatg ccaaaactcg
    29821 caaaattatt ttctaatata attatagtta ccaatcaaca atgcatagca aaaggtttat
    29881 tgacgcacga acaattgaac gaaattcatc aaaatatgat gaatatgata gaattaaacg
    29941 gtggaagaat tgatgccatt tactatgctc cagatttcgc atctgaggaa aacaccttgc
    30001 gaaaacctaa aaacggaatg gcatttttag caaaagatga ttttcccgaa atcgacttta
    30061 gcaaatcaat tatggtaggc gacaaaccat ctgacatgga atttggaaaa tctataggta
    30121 tgacaaacat ctacatttct aagaatgcaa cacaatattc attagcagat tacactatca
    30181 cacagttttc agacctatta caagtaataa aatgctgatt ttggtcaaaa aagttggcac
    30241 agatattgac ttttaaatat caattaacta tatttatggt tgataagatt gtaaatacat
    30301 taaaaattgt gaataagcct atgcgtagaa cgtttacact tatactttta ttgtttgctt
    30361 cgagcctatt ttttccgagg ctcagtcaag cattttctgt tgctaatatt agtgcagaag
    30421 ctaaatttgg cgaaaaggat ttaatcaaac tttatcctaa tccgatgaca actgatgcta
    30481 ctataaaaat tagtagtgac atcgatgtag aaagaagcaa agtagctgtt gtcttttaca
    30541 atatgattgg tagcgaagta tatcgcattg cgcaagtaaa agaatatgat ttaaaaatca
    30601 atcgtgatat tttcaaaaat gcaggtatct attttttcca attaaaaatt gatgaaagtg
    30661 ttgtgagcac aggaagaata accgtgaagt aaccactaac actaactatt atatcgagtc
    30721 caatctttaa cgattggact ttttttatcc atttaatttt gcatagtttg tcttctcaca
    30781 agcatattct tgcacaaaga aatcatccat ttcatttgct tttattataa gttcgcttag
    30841 attttgttgg ataattgtag catttgcaga aaaaacatca ctcatgcgaa ctctatagga
    30901 aatataaatc tgattattat acactgccaa tcgataagga ggcaccggat tactattcac
    30961 atagcgaagc aaagactcca aatttccttg tggtaaatta ttaatcggcg atgtagcata
    31021 tagataattt ttatcataca caaaaatgcg caccaatgca ctaccagaat ggaattccca
    31081 atattcatta ccacaacgag ccaaaacagg atctatgccc atatttttaa tcgctgtttc
    31141 gatgtataaa gaaagtttgc ttatctcttt atcatcaaat attttttcat cgatattcgc
    31201 tccacaatta ggacagtaat ctgttttttc tacgattaat gatttacaag aattacattt
    31261 gagcgaatag gctccacttg tattggtagc aaaagtatcc aactcttctt ttaatacgct
    31321 ggcaggaatg gcaaatccaa cattatctgc ttccgtaaat tttgctgtag ttacaccgat
    31381 taacaaacca tcagcattga caactggtcc gccgctattt cctggattta cggctgcatc
    31441 tgtttgtata aaatggcgac catctaacaa ttgcttttcg tttgacacga taccttctgt
    31501 aatcgtataa ggcatgccaa acggaaaacc taaaacatat acaacatcac ggctatgcac
    31561 agtttccact gtttcaaacg gaataaatgc atcaggctgg tagtttttac ctcgtagaaa
    31621 tgcgatatct gcttcagggt ttacataaac gacatctgct atatatcggt cttgcaactg
    31681 attttcgata gatacttgtt tattgccatc gataacgtga taattcgtaa caatgatatt
    31741 ttggcttttt aaatagaagc ctgtgccact gccactcgca gtatttattt tgtaaacagc
    31801 ctgttttaaa ccttcgtgat tcatatttac attttagcca aataatttat tgaaaaaacc
    31861 ctttttcggg cttggattga tatcatctgt ttttggtaat gctgcaacag attgtaaaaa
    31921 cgcatctgat gtcattactt tttttaccgc atcaaaaagc aatgtcgctg tttcttgcat
    31981 ggatttgcct gcagcatttt ccatcgcatt gcttaattca ttggctatgg catcttcaat
    32041 attgtgatta taaaataaat ttaaaatgcc gtattctaaa gtgcagcttg ctccaataaa
    32101 atctaaagat tttatttctt tttcagatgt ctcatacgca tactttaata tattgcgcac
    32161 accgtctaaa ttggtgcgaa atttataagg cacaaacact tcgattttat ataaatcatc
    32221 atcaaacttt gcgcgttcat attgttgtaa tttttttaga aattctttgc ccttgctcaa
    32281 tcgctgataa taatcttctt ttgaatttaa aaaggataat gttttttcaa tctcactacg
    32341 caatgtacct tgcggtgcac aataatagtc gattttcaaa atagaaatca tgagcacatc
    32401 ccacaaatag ctggttaatc gtttactctc taactggtca taagcatcaa acgcttcgac
    32461 aatgtattgt tgaatttttt gccaagcctg ttctttctgc tcggcagtat atggtgttac
    32521 acgtggttct tgaatgcgtt ttgcattaaa tttattgata aattcatcgt cataattgtc
    32581 tgcattgata cagatttctc ttagcacatc atacactttg tacggctcag ctacatttaa
    32641 tggacagcta tatttaaaat atagtttttc attttccaat actatagaag atatacttaa
    32701 cggcgtaaaa tttaactgcg ctacttgtct taaaagcggc actattttgg cgccttcaac
    32761 atacaaaaac ggagcattta caacaaaact atccttcgtg atttgcaatt ctaccaatat
    32821 cgaaccatgt ggaatgacat aagttgtttt gtcggcattg gcatatttct gttctatcga
    32881 tggattgata taattaatac aattgagaat ggcatcgcca taatcacctt tgtcgtatgc
    32941 agccaacact ttatcccaaa tagaaggatc tatggagctc tgtaaagaac cagcaattgt
    33001 ttgatgaaat gacaaatttt tctgcatata attatattga gcgactaaaa taccatttta
    33061 ttatcttttt ttacctaact taaggatagt gagtattttg tatttatttc ttacttttga
    33121 tgaaatgata aaaattctta tagcagacgc tcaatatcta atcagaattg gattgagaaa
    33181 cctattgtcg tctaataaag attttaaaat tattggtgaa gcttcaaatt ctgcggaatt
    33241 attacaattg agcaaagagt accaacctac agtcattatt tttgattaca atagtgataa
    33301 aaattttgtg ttacaagatt tagaaagcat tcgaaaaatt tctcctaaat caaatttatt
    33361 agtgatttct gccgatgatt caaaagcaaa tatttataaa gctattgaat atggtgtgat
    33421 aagcttttta acaaaggaat gcgataaaga agaaatcatt gctgccattt acgcagccgc
    33481 taagaatgag aaatttcttt gccacaaagt tatcgatatc atcatcgaaa aacacatgca
    33541 tgtagataat gacaattgta aagcatttaa tctttcttca cgagaaacag aaattataaa
    33601 actcaccgcc aaaggttgga ctgcgaaaac cattgctggc catctttttt taagcactca
    33661 taccgtttat acgcacaaaa aaaacatcat gaagaagttg aaaatcaatt cctcttcaga
    33721 aatgattgtc tatgctatac aaaatggatt gattagcaaa gaagaattct ccgaagccaa
    33781 ttaataccta atcttaggta taatatttca aatttttatg atagttttta attgcaaaat
    33841 ggtttatttt tgcattctat tttgaattaa tctaaataaa aacaaaaaaa atgtatctat
    33901 taaaccttcg aaatgcttgc atttttgcaa ttgctgtttt atttgtttct tgtaaagaag
    33961 aaaaagcacc attaataatt cctacaacat acgatggtgc aaattttgat gcaaatgtaa
    34021 gtgatgaaag tagcttaaga acgcaattaa gtgctttatc ttcttatatg aaaagagcag
    34081 aaaacgtatt gaataaatta gaagctgatt ctttgacaaa gtattatgct aataacggaa
    34141 caccttcact taaaagcatt actgccacta gctattcaaa taatattgaa aatagctatt
    34201 tcccaacaat ggttacatgt agtcaaaacg tgtatgatgt tgcattaggt gctgtagcat
    34261 tgaacggcgg cgtttatggt gcaagattat tagacaaaag aggaaaagaa accttgcaag
    34321 aagtagaaaa aggcttatac gaagcagcac tatacaatca tttagttaca ctttcgcaag
    34381 gaactatcac aacagcaact gtcgataaaa tgattcgcat ttatggtgca catgccaatt
    34441 tcccaaatac gaatacaata gcgaatacga gcacaccaga tgcatttatt gctttatata
    34501 ctgcacgtag agataaaaac gatggtactg gtttctacac taaaattaga gatcaattca
    34561 tcaaattaca agcagccgta aaagctggaa gtgaatataa tgatgagaga aatgaggcaa
    34621 ttgcatcgat taaattatta atagaaaaat cgattatggc tactgttatc aattattctt
    34681 atgctgcaat tcctaaattt acaagcggca ctcctacagc atcttcaaat gcaagtgcaa
    34741 ttcatgatat atctgaaagt attggcttta tccatggttt taaagccata ccagcagcac
    34801 atagaaaaat aagtgatgca caaatcgatg aactcttagg tttgttctat gcaccatcaa
    34861 atgctgatgc ttctgtgtat aaatttgtaa caaatcctgt gactgaaact acaagaatca
    34921 gtgatgttat tacaaaaatt caatctatat atggatttac aaatgcagaa ttaatcgact
    34981 ttaaaacgaa ttggatatcg gcacaaggca gataattaat aataaaatac agaattgata
    35041 atgaactatt tgccataaaa aaacaaatag ttcttatctt tcaagacagt ttttatatat
    35101 gaaaatgatg ttgaaatatc gcttaataat gcttatgtgc tttgcatttg ctattgcttg
    35161 taaaggacct gaaagcacgg atgaattcgg caaaaaagaa atgtttatca actacgctga
    35221 caatattatc ttaccagctt atacaagtta cggtacgaca ttaaataatc tacaaacagc
    35281 atttaccaca ttttcgacaa acacagatgc gacaaattta agcgcattac aaacagcctt
    35341 tttaaatgcc tatgccgctt ggcatacttg tgaaatatat gaaaaaacac cacctgctga
    35401 aaatgcaatg gccatcgaca acaataatta ttttcccact agaaaagata gtatagaagc
    35461 gtatatagga agaaattcaa catcactttc atttgtacaa tcaagaataa aatcggacaa
    35521 aggcttgcct gcaatggaat atctcttatt tagcagaaca ctaacgccaa cacaaatttt
    35581 agagcgcttt acaattcatc caaatgcagt agcttataaa gcttatttag gttcactcat
    35641 tagcaattgt ataaatgtgc aggctagtat cgttacagaa tgggcaactt atagagatac
    35701 ttatatcaat agccttggca cagatgccgc aggttctatt agttatatgg tgaactcatt
    35761 cgctcaacga gttgacgatt ttaaacgcca tcaaataggc attccggcag gctatcaagg
    35821 caatgtggca acaatcaata taaatcctca agctgcgcaa gcgtataatg ccaataaaag
    35881 cattgaatat atgttgctta ctttaaacaa cctaaaagat gtgctaaatg gaaaaggtgc
    35941 agtagatgga aaaggattga atgactacat cagaactctc aatgttacta gtacttttgg
    36001 tggcaatctt gctgatgata ttttagcaca aatcgatgtt tgcatcgaca aagtaaatgc
    36061 ctgcggtgtt gactatgcca atacgattac gacagacaaa ccaaaagcag atgcactttt
    36121 tttagaagca aaaaaactac ttgtattaat gaaagttgat gtgccttctg ccttaggtgt
    36181 ttccattaca tacacagata gcgacggcga ctaaacaccg attttaatca tgagaaagca
    36241 aactcaaaaa acattctatt ctttttctta tgattgctaa tgtaaaaaat tttctttatc
    36301 gtccagtttc tattgcacca ctagcatcat tccgcatttt atttggtgcc atgttgctgt
    36361 ttagcagtgt gcgatttgta gcaaaaggct gggtcgattt attatacatt caacctcaat
    36421 tttttttcgc atacgaaggg tttgaattca taaagccact tttaggaaat ggaatgtatt
    36481 ttgtttttgg tggcatgata ctagctgcta tttgcattct atttggcttg ttttatagag
    36541 tagccagtat cgcatttttt ttccttttta cctatgttga attactcgac aaaacaaatt
    36601 atctcaatca ttattatttc gtcagcatca tcagtttaat gatgtgtatt ttgccagcca
    36661 acaaacacgc ttcgcttgat gtaaaattgg gaattacttc aacaagtacg tacattccta
    36721 attggtgtat ttttatttta caattgcaaa ttgcttgtgt atattttttt gctggcattg
    36781 ctaaaataaa tccagattgg cttttgcacg cacaaccatt aacaatatgg ttgaaaagca
    36841 gagaccatta tccaatcatc ggatttttat ttaattattc aatagtgcct tacctatttt
    36901 catggttcgg tatgctgttt gacattagca ttgccttctt cttatggaat aataaaacaa
    36961 ggccatttgc ctatattgcc gttgtcgttt ttcatgtaat cacaagttat ttatttcaaa
    37021 ttggtgtatt tccatttgta atgatagtgg ctacctggat atttttttca gccaatttcc
    37081 accaaaaatt tttatctatt ttctcgatag caccaattca aaataaagta atatatatac
    37141 caaaattcaa ctcatttttt gttgcactac tctgttttca ttttttaatt caattgctat
    37201 taccttttcg atatgcattg tatccaaata aactattttg gacagaacaa ggttatcgat
    37261 tctcatggcg tgtaatgttg atggaaaaaa atgcgctcac cacttttgtg gtaaaagatg
    37321 caaatggaaa aattgaagaa gtatgcaaca gaaattattt gacaccacaa caagaaaaac
    37381 aaatgagcac acagcc
//
LOCUS       DAOQQV010000013        36459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_323_length_36459_cov_7.684842, whole genome shotgun sequence.
ACCESSION   DAOQQV010000013 DAOQQV010000000
VERSION     DAOQQV010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 36459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36459
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_323_length_36459_cov_7.684842"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLL59_02745"
     CDS             <1..211
                     /locus_tag="PLL59_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HMY22751.1"
                     /translation="HQVIAITHLPQVAARANRHFFVYKNHEKEKTFTEIKTLQGNERL
                     HEIAVMLSGNNPPKEALENAKRMLN"
     gene            complement(231..1475)
                     /locus_tag="PLL59_02750"
     CDS             complement(231..1475)
                     /locus_tag="PLL59_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HMY22752.1"
                     /translation="MILNYIWILFFVLSFIIILVKMLIFGDMTVMPASINAITDSSKT
                     AFEISLGLTGVMCFWLGIMKVGENGGAINIFSKLISPFFSRLFPEIPKDHPAMGSILM
                     NYSANMLGLDNAATPLGLKAMKELQEINPNKEQASNSMIMFLTLNTAGFTIIPVSIIA
                     FRATAGAANPTDIFLPLLIATYGGMIAGLILCTFIQKIKWDFVLTAWVLGIVGFIAGL
                     TYLFSTMPKEQMEIVSNVLGGLIILTVIITFIAFAFFKKVNVYEAFIDGAKEGFDITI
                     KIIPYLVAMLVGIGIFRACGGMEIITHGIAYCFNSLGFDTQFVDALPVAIMKPFSGSG
                     ARGLMVDLIKPVTEGGLGADSFAGRLAGIFNGSHDTTFYVLAVYFGAVAIKKTRYVVA
                     TSLFAELVSIILSIVVCYVFFG"
     gene            1554..2018
                     /gene="smpB"
                     /locus_tag="PLL59_02755"
     CDS             1554..2018
                     /gene="smpB"
                     /locus_tag="PLL59_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HMY22753.1"
                     /translation="MSKKYELPEIVNRKAKFNYEFLETYKAGMVLTGTEIKSVKEAKV
                     NIGDSFCYFRQGELWLKNLHISEYKNGSYANHEPLRLRKLLLTKKEIKKLEGKIKEKG
                     LTIIPFKLFFNERGLAKIEIALARGKKSFDKRESIKERDTKKEIQRVQKSMR"
     gene            2215..2415
                     /locus_tag="PLL59_02760"
     CDS             2215..2415
                     /locus_tag="PLL59_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22754.1"
                     /translation="MKQNLITLTLFLLVHLSFADVPIDDNEGTLSMYSNANFLPLILG
                     VATAIAIFVFMYVYKNKVNEKK"
     gene            2402..2962
                     /locus_tag="PLL59_02765"
     CDS             2402..2962
                     /locus_tag="PLL59_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22755.1"
                     /translation="MKKNKLIILFSLLVLKLSAVADIALPKIENLQETKYYFANVDSF
                     ANYTFYVKKNANDKTYRIKQSSAFLLKDNSKIGEEQIEVWAVRNSDKQKSNTFVLKRV
                     KPAIEHGGTTAHIAIMLSFDKKNNLNYKQVVMKPECYKRNYIVPIIPFTFTPTNSLLL
                     LVSIISMLILFSLAMYKPVFRRMKYA"
     gene            2955..3263
                     /locus_tag="PLL59_02770"
     CDS             2955..3263
                     /locus_tag="PLL59_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22756.1"
                     /translation="MLKYLALTIAIEIPIYFLFDRNKIVYSTFVLFLANCFTWPIVNI
                     LFHNTNIHLLILESGVTIVEAIIINIFLQDKFSKSLLISFIQNSITTFIGIWINNIQL
                     "
     gene            3260..4315
                     /locus_tag="PLL59_02775"
     CDS             3260..4315
                     /locus_tag="PLL59_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMY22757.1"
                     /translation="MNNKYKTATLFGMAHGLNDFIAGYLLANLSSHSSDIFTNSLAYL
                     MYSVIAFGGQLPAGIIVDKYKRIKPIASICLLLLILSISVSYASLFMAIFFSAITSAF
                     IHVCGAAACYVSDNKHYTLAGIFTSPGVIGLICGGILGSIDFNYFYLFILPLVVLLLW
                     LMYVEIPSYENKKVNAHSSDTILDTHDMVMLLLLMTIAVRSLFWNVVHMMSFNQQTWL
                     LVLGFSAGAGKLSGAIIADRVGWKKFMYVSIIGAVICLNVDKSNIVLFGIGVFFLQSA
                     VPITLVLVQQYMKQSPATAAGLCLGVLILFAGLPSYFHAFRQLQTHQHINVVLSALFF
                     LSNFCFLLMYQRKAKYQ"
     gene            complement(4302..4691)
                     /locus_tag="PLL59_02780"
     CDS             complement(4302..4691)
                     /locus_tag="PLL59_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMY22758.1"
                     /translation="MKNGTKLIDIGTRIIAAGIFSYSAFMKLTAAPAAVHIFQRINME
                     PAGRFSVAGLEILAIILLLIPKMAWRGALLGMVLMLGAIVVHIITNEFDVLGDGGLMF
                     ASAIVVFFSCISILLYQADMETSVTDI"
     gene            complement(4877..5122)
                     /gene="atpC"
                     /locus_tag="PLL59_02785"
     CDS             complement(4877..5122)
                     /gene="atpC"
                     /locus_tag="PLL59_02785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HMY22759.1"
                     /translation="MTLNILTPELNVFSGEVDSVQLCGSNGKFEILNNHAPMIASLVK
                     GTVRIKSAAGKQEFNLKSGIVEVLKNNVSVLTEGIIE"
     gene            complement(5201..6706)
                     /gene="atpD"
                     /locus_tag="PLL59_02790"
     CDS             complement(5201..6706)
                     /gene="atpD"
                     /locus_tag="PLL59_02790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HMY22760.1"
                     /translation="MANVGKITQIIGPVVDVTFGGEGASLPNILNALIVKKDDGSEVV
                     LECQQHLGEDSVRCVAMDATEGLTRGAAVTDTGNQITMPAGEAIKGRLFNVIGTAIDG
                     IGEVSKEGGYSIHRKPPKYEDLATSKEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVGK
                     TVLIMELVNNIAKGHGGLSVFAGVGERTREGNDLLREFLESDVIKYGEAFKHSMEKGD
                     WDLAAVDKTALLESKATLVFGQMNEPPGARARVALSGLTIAEQFRDGDGDGKGKDILF
                     FVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLSTEMGLMQERITSTKSGSITSVQAVY
                     VPADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILDPQVIGERHYN
                     VAQRVKSILQRYKELQDIIAILGMDELSEEDKLVVHRARRVQRFLSQPFHVAEQFTGL
                     KGVFVSIEDTLKGFEMIMDGQVDEYPEAAFNLVGTIEDAIEKGKKLLAEAK"
     gene            6873..8147
                     /locus_tag="PLL59_02795"
     CDS             6873..8147
                     /locus_tag="PLL59_02795"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMY22761.1"
                     /translation="MFFPKWRPFLLVLVFSKSFISFSHQDSIKCATEFNFINKTIDNQ
                     YVMSGRLAFDQRFRQWQENNIQNRNSSISSLITIPVVVHVIYNTATENISDAQIQSQI
                     DILNKDFRNLNTDKLASTHAFYNLSGDAGIEFCLAKEDPIGNAVSGITRTYTSLQEFG
                     NESNMKYAALGGVDAWDTKRYLNIWVCNLGKQLLGFATYPTAFEYAPNEDGVVLNFTA
                     FGIMGTAKAPYNNGRTATHEIGHWLSLRHIWGDDNNSCDDDDGIDDTPNQFGATAGCP
                     SGVLVDGCTVSSTGVMYQNYMDYSDDACLVMFTKQQIAKMRFVFDEIRTEMLSSPICS
                     RTTGIANQVHSKVNIYPNPIQNYLSIEGLPKTRNKMYSVELFNMIGELVFSTAIPADD
                     TLIEMTELPIGTYVLKIYNNDFTTIHKVTVVK"
     gene            complement(8156..8737)
                     /locus_tag="PLL59_02800"
     CDS             complement(8156..8737)
                     /locus_tag="PLL59_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMY22762.1"
                     /translation="MEINDANNIEENLLLAAKNGHQKAFTALMEKYQKSVYHLILKIV
                     KSPEDAEDLTIVAFSKAFDHIDQYSADYAFSTWLYKISSNTCIDFLRKKNVPKISLDD
                     EAKNITDHLKYSTNSTPEYDMIKAQRIDKLQAAVNTLDTVFSRVLTLRYFKEYSYEEM
                     AEELGVSVGTIKVQLHRAKKKLMENFKTEIESW"
     gene            complement(8728..9807)
                     /locus_tag="PLL59_02805"
     CDS             complement(8728..9807)
                     /locus_tag="PLL59_02805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY22763.1"
                     /translation="MMLFFICCVLFQVIYQCILLCALIKKKNKIIAPLQQFPSVSIVI
                     CARNETENLTKNLPFIFHQQYPDFEIILVDDGSDVPIQLQHERLTIIKIDKNEKIGIG
                     KKYAIQKGISQAKNSLIVLTDADCKPLSNKWITSMVNGISDTHKIVLGISPYQKTDTF
                     LNGIIEYETAQTAWQYCGFALLGMPYMSVGRNVIYDAKLLKSKSWSTQEFAIASGDDD
                     LAIQSLSTAGNTSVSLSLDSYTISEAKQTLKDWYRQKIRHYESGHLYKYAHQLVLGAY
                     LVSKFLMYFLCLIIIISCSVFSLYFLGILIGYFIIITMFNFQLHKKFQLNHRWYFSAI
                     NDILYSIFTTVFGIISFIKSTRDWK"
     gene            9867..10997
                     /gene="tgt"
                     /locus_tag="PLL59_02810"
     CDS             9867..10997
                     /gene="tgt"
                     /locus_tag="PLL59_02810"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMY22764.1"
                     /translation="MKYEILHTDKHSSARAGKISTAHGDIETPIFMPVGTQGTVKAVH
                     FTELETDIKAQIILGNTYHLYLRPGLEILQKAGGLHAFNTWQKPILTDSGGYQVYSLS
                     HRRKLNEQGVTFQSHIDGSRHLFTPENVMDTQRIIGADFIMAFDECTPYPCEYKYAKK
                     SLKITHDWLQRCIHRFDTTKPLYGYEQTLIPIVQGSVYTDLRKESAAFIAAQNRPANA
                     IGGLAVGEPAEMMYEMTEIVCNILPKDKPRYLMGVGTPANILESIALGIDMFDCVMPT
                     RNARHGLLFTTAGIINIKNQKWKDDFSPIDINVPASTSQKHSKAYLRHLFVVEEYLAA
                     QIASIHNLSFYLWLVNQAREKIKEGTFTTWKNEMIVKLSTRL"
     gene            11035..12114
                     /locus_tag="PLL59_02815"
     CDS             11035..12114
                     /locus_tag="PLL59_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMY22765.1"
                     /translation="MKILDRYIIKKFLITFFFAIFLLSAIAIVIDITEKIDEFIDNQA
                     PLHAIVVDYYLNFIPWIGLMLAPIFVFISVVYFTSRLTANTEVICMLNGGVSFYRLLF
                     PYLFTAVLLAMLFDYYNHELLPKNNKVKIKFEEQYASKKNQRSADINYHFQIGKDTFI
                     YTQSFDINANTGFKFTLEEIKNKVQHYKFTSENMSWDTTAKKWRMRNWTFRKSLGNRD
                     SIGKGMDSLMALPVTAKEYAKQNYDIETMTTKELKQFIIDQKHKGNENVKIYEVEKFR
                     RTAIPFSTIILTLIAVAMTTKKVRNGMGLYIVAGMLLSGAYIIIQQFSTVFATKGNLD
                     PLIAVWIPNIIFGIIAALLIKRAPK"
     gene            12214..12477
                     /locus_tag="PLL59_02820"
     CDS             12214..12477
                     /locus_tag="PLL59_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HMY22766.1"
                     /translation="MLFGINLNNLTDKKNITQLQIGESALITAFADGQIAAKLMAMGL
                     LPGTTLALLHKAPFGGAYYIKINNHYLALRVNEAKNVLITSAS"
     gene            12477..14585
                     /gene="feoB"
                     /locus_tag="PLL59_02825"
     CDS             12477..14585
                     /gene="feoB"
                     /locus_tag="PLL59_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HMY22767.1"
                     /translation="MSVQKYKIALLGNPNAGKSSLFNQLTGLRQKVGNFPGVTVERKS
                     GIIKDNAINLEIIDLPGTYSLYPTSLDERIAVHALIDSKNRDFPDAIIYILDVTHLER
                     HLLLLTQISDLQFPIVVGLNMNDIADQQNMYCDEKILSQALGIEVIKINGRTGENMAL
                     LKSKAINLLKQPISNRQVFYTINEQEKNIAKDVALLLPVRNEYHALLLAHHAKRSTFL
                     VQNIKQQLLQINTKHQFNPINNQLDEIMQRYNAFVPIVNKVLKRSFDNAKTLTDKIDR
                     IVTNNYFGPILFFGLMLFVFQAIFAWSAFPMDWIDLQFANIAASLQASLPDNLFTRLL
                     TEGIIPGISGVLVFVPQITILFLIITVLEEVGYMARAVYMFDHIMQKFGLNGRSIVSL
                     ISGGACAIPAIMSTRTISNWKERLTTIMVTPLISCSARIPVYAVLIGFIVPSYKLGIF
                     NAQGLVFMALYLIGIVSALIASLVFKKVLKSTDVSFLMLELPAYRKPHWKNVFFTLYE
                     KVSSFVFGAGKIILIVSVILWFLASFGPSTAMKTAENTAIIESSLMQADSATTQLHIA
                     SKKLEASYAGHLGKCLAPIIQPLGFDWKIGIALITSFAAREVFVGTISTLYSIGKDAD
                     NLTIKQKLQQEKNADGQPVFSMAVALSLVLFYVFAMQCMSTLAAVYRETKSWKWPAIQ
                     FAYMTGTAYLVSLLVYQFLK"
     gene            14725..15288
                     /gene="msrA"
                     /locus_tag="PLL59_02830"
     CDS             14725..15288
                     /gene="msrA"
                     /locus_tag="PLL59_02830"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMY22768.1"
                     /translation="MNTTANSSSSAMDTATLAGGCYWCLEAIFQRLQGVEKVTSGFCG
                     GQVKNPSYKAVCTGTTGHAEVIQLTYDSSVISFAEILKVFFTMHDPTTLNRQGNDVGT
                     QYRSAIFYHNTTQKKEAEEIIHALNMASVYSTPIVTTVEPFDVFYIAEDYHQNYYNDN
                     SNEGYCRYVIQPKLEKFEKVFSDKLKK"
     gene            complement(15302..16099)
                     /locus_tag="PLL59_02835"
     CDS             complement(15302..16099)
                     /locus_tag="PLL59_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HMY22769.1"
                     /translation="METILQDISIQQALIIILNLILIESLLSVDNAAVLATMVMDLPK
                     EQRKKALKYGIIGAYVFRGICLVLAAWLVKIWWLKALGGLYLLYLFIDYFRTKATPKP
                     EDDTLNKEESFIYKYTLGLFGKFWSTVILVEIMDLAFSIDNVFAAVAFTDNIYLIWTG
                     VFIGILAMRFVAQSFVKLMEKYPFLEKAAFIVIGILGVKLLLSIPAHYLKDSTTFKYI
                     ESEHFDLIVSIITVLVFFLPVISSILFNYPKRNLSASNDEQEQSDES"
     gene            16198..19131
                     /locus_tag="PLL59_02840"
     CDS             16198..19131
                     /locus_tag="PLL59_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HMY22770.1"
                     /translation="MKKILLLFAAISIAFFSFSQTKYFPTPGASEIPGSKINGKLDDI
                     TDFSTCTQVPFTMPDGTKLMTDIYLPILQDSFVYNDTLTFSLLPDPFPPIKIPIYAVL
                     LKKGQQYLIYDSINGVPNPNPYQLPLILERTPYDKDGAVEGAAMALLGYAGAVQDMRG
                     RYTSQGAYLPLYSDSWKKWPYHNYKHVLDITDASDPRNGNNHEDGYNTIEFIKNQLVR
                     KYDLNRDGNFETTDLLYNGSIGSFGASALGYNQLQAAAAHKIDPSQPGLKSMFPIVGP
                     LEFYKSTGYQNGCLRDGLVTGWLRGQIKDTRDDLWNIDSGLNDSIHSSKDYNTVDKFE
                     ASNKAIDHFVTVQYENSPAGYYPNSIGRSDMDASKAPVDINGEGAKNGAYSRYKNIEV
                     PTFHVAGWWDIFVDGSLETHRFIQDNITQKKNLQKIIIGPWAHQTIASTKTGDKVYPK
                     NVTDITKIDITNIRGFEDLDIAELAKSELISWFRYNLNDNGYNKVGDPKVLISKTSEW
                     QAPLPQLPQLEVRFPSEDYKIQFADLLNFILGQDNLKQVPVEVRLGGSSGLVIPFKID
                     LPDMGPLIEGFGSNGAFTGIPKYDYTTIPSIRYYVTGASSDDGTVSYAGNYWMGSETF
                     PPPSVQWHSMFLHQNGKLNFTKPTSDEGYRTYVHNPDDPIITVGGGNMLERTPQGDRD
                     AQGQMNLADTNFAKFTMDREGVLKFETDVLQDSLCIIGFPKYKLYAKSNPGNTPSGPT
                     DCDFFVRILDVYPDGKEFFVVEGAVNARAREYAKSIAVGAENDNAPFSNIDIGKIYEY
                     YFQGYPIAYTFAQGHKIKVLISSSNYPRFQACANVPITPNDFFRRKPADGRTYMFNGV
                     EYAPRISVQRIAFSDQYPTQIELPVFGNSSLVTALKNNSTKPTWDINLFPNPSNGAFS
                     VFISKNGKYIANVYNMIGEKILSREIVEHQSFNMKDLAKGQYLLEIFDVKNTDQKQTK
                     SFTIN"
     gene            19161..21986
                     /locus_tag="PLL59_02845"
     CDS             19161..21986
                     /locus_tag="PLL59_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY22771.1"
                     /translation="MHTTGNFFKFFTVLFCLFSNIYLYAQEITISGQVIDKNTKEPLI
                     GANILVKGTTIGATTDIDGNFIIHGDIRQPVVIGSYLGYATQEITVTEGNKKIVIALA
                     PQEIRMSNDVVISASRLSERIQTSPSSIQKLNAKQIQSVASGNFYQSLGNLKEVDITT
                     TSMGFQVFNTRGFNTTAPVRIVQFIDGMDNQAPGLNFPVGNLVGANDLDLESVEIISG
                     ASSALYGANAFQGIVSMQTKNPFDYKNLQVKIKGGNRQLIDAQVRYANAFGKKKYGRD
                     VFGFKVSGSYFRAKDWIADDTIANVYGDIGTDVNVSTVVRKLQYNEDTAIARQFRALN
                     AYLDFFPNAFPGTIHVQAPGYREKDLTDQQTESIKASAALYARPIKDMQIEYQYKFGR
                     GSAVYQGANRYNIKNILFQQHKVQLDYKGLSVKYYTTQENAGKSYDMVFSAINLSKIG
                     ITRYVSNFVKAYFSKLSDFTNTFKDDPEDADVQTSREYALQEALNNAYLQPGTFSFDS
                     AYNIIVDDANLVTGSKFQDKSSLHHVEANYNHSFKHDINLIVGTSYRMYIPRSYGSIF
                     YDTLANRADTLADGSPDLDAKFINLNTWEVGAYTQISADLFKKHLKLVGSARIDKAEN
                     FKVQFSPRISLIGTYKDHTFRFSYQQAFRSPTLQNQYISLDLGAIQLLGNLRGAEGYD
                     FQSVKDFRSNYDVTLEVDPTLLKPIKLNPIKPEQVNSWELGYRGVFARKLYVDVSAYF
                     NRYFNFIGDLRFYMPENKDIVVGTEAGADAMLTGAYNLIQMPTNAKQKVDAFGASLGL
                     NYYIWKSLIGSFNYTYSGINTKNLKDPIIPGFNTPNHKFNIGIAAQRIWKGLGFGVNF
                     KWVSKYNWESTFGDGAVPSYHMLDMQVSYEFPRWFTLQVGGSNIYNNKHIEAYGSPMI
                     GALVYGSMLFDLERKGNVEERWIKKKK"
     gene            22079..22795
                     /locus_tag="PLL59_02850"
     CDS             22079..22795
                     /locus_tag="PLL59_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY22772.1"
                     /translation="MSKKLTIVVPAYNEENTIQNILNRINEVQLIQQIQKEIIVVNDC
                     SKDKTEQKVQAFQQQHPELNIVYKKHEVNKGKGAALRTGFQSATGDFVIVQDADLEYD
                     PNEFNILMKPILDGFADVVYGSRFIGGKPHRILFFWHTIGNKMLTFASNVFTNLNLTD
                     METCYKMFKREIIQSIDLKENRFGFEPEVTAKVAKIAKVRIYEVGISYYGRTYEEGKK
                     IGMKDAFRALYCILRYNLFS"
     gene            complement(22784..23308)
                     /locus_tag="PLL59_02855"
     CDS             complement(22784..23308)
                     /locus_tag="PLL59_02855"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMY22773.1"
                     /translation="MSIALAEKYQEIETIKNKFLQKVANLNAEQLNKVATNGGWSAGQ
                     TLYHCSFAESGTIIVMQKNLAENKVQLQSSIGSVFRNILLVVLLKLPIKFKAPKVVSS
                     VPEHVSIKEIQDYFAKNTNDFKQLLINLPLELEDKYIFKHPRSGWFNIQQVLHFVSEH
                     YAHHERQLDEVLGK"
     gene            23366..24553
                     /locus_tag="PLL59_02860"
     CDS             23366..24553
                     /locus_tag="PLL59_02860"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMY22774.1"
                     /translation="MFLDFFLLLKEEGLPVSIGEYLNLLEALNKKVTQFNVEEFYFLS
                     KTILVKHEQFLDRYDLLFGNYFNGIEISEAEKKEIPADWLQKEIDRLFSEEEKAMIEK
                     MGGLEKLMERLQQLLEEQKEKHQGGNKWIGTGGTSPFGNNGFNPEGVRIGGGGGGRTA
                     VKIWEKRQFQNYAADVELNTRNIKLALKRLRILTREGIEDELDLHTTIEKTSRNGGYL
                     DIEMQPAKKNRVKVLLFFDVGGSMDPYIELCEQLFSAAKSELKHLEFFYFHNCVYESI
                     WKDNNLRYTDRIPTFDILHKFNQDYRVIFVGDATMSPYELTALGGSVEHYNDETGIAW
                     LQRFVTKFPNIVWLNPSNEYYWDGVPTIKAIKEIFKDRMFPMTIDGLSKAMKTLKGKK
                     IIH"
     gene            24846..25700
                     /locus_tag="PLL59_02865"
     CDS             24846..25700
                     /locus_tag="PLL59_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMY22775.1"
                     /translation="MNEFKGTENYIATDELQVAVNAAIALEKPLLIKGEPGTGKTLLA
                     FEVAKALNKQIFTWHIKSTTQAQQGLYEYDAVARLRDSQFGDEKVNDIAHYIIKGKMW
                     QAFESEEQSVLLIDEIDKADIEFPNDLLLELDKMEFYCYELQKTIKAKTRPIVIITSN
                     NEKELPDAFLRRCFFHYIRFPERQTMIDIINVHFPKIEDELMDEALKVFYGLRDIHGI
                     KKKPSTSELIDWIRLLKLGKNTKEDLQKIDYVNAQPPYFGSLLKNEQDYEHISKVKRA
                     GQNTFKRF"
     gene            complement(25710..27152)
                     /locus_tag="PLL59_02870"
     CDS             complement(25710..27152)
                     /locus_tag="PLL59_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22776.1"
                     /translation="MNNVSSQKRIAITIAVLFFVVMIVIAYTYSGTGDEGDSIMHFLF
                     SKYAFEHTHHFFHHWAKPVYVLITCIPAQFGFIGIQVFNIFINALSIYLSFCVAQKLG
                     IKHAWLVPLFLAVFPHQIIYSLSGLTEPLFAFALILLIHLWLYDKKIIALLLLSFLPF
                     IRSEGLLVMVVFFIYLLLKKEWKILPLLMVGHVVYSLLGFIIYDKSLLWIFNENPYAV
                     GTTSYGSGPWDAFINNIRFSTGIILYYILWLGMLLGLIYSILKFILKKEVAFTLEFFF
                     LIYGIFFSVFFFHSFAWYKGIFHSFGLIRVMVGVLPLISIICAKVINDIFLFIPETKY
                     PSHTIGVLIILVVATNFGYTKYAVIPDRDLQARGDQKCQNEIGNYLWQYHRDCYNLPM
                     YYAAPYISIVLNRDYFDQKIHPDFRNDFHNNVMKKPCYVLWDDWYAPVECDVKLEDIE
                     TNPSFKKIKKFWHWDINSGKDRVTILYKIE"
     gene            complement(27255..28322)
                     /gene="pdxA"
                     /locus_tag="PLL59_02875"
     CDS             complement(27255..28322)
                     /gene="pdxA"
                     /locus_tag="PLL59_02875"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HMY22777.1"
                     /translation="MSNQNQKRKVGITLGDVAGIGPEIIIKTFSDELIYKHFTPILYG
                     NPRVLSYYKKMLNIDKFQYSSIKGYGNLSHNSLNIIPVWEEEFQITPGTPTDITGNYA
                     LKSLEAACDALKNKQIDVLVTAPINKNVIHSDKFPYAGHTEYLMNYFGAKDNLMFMVA
                     DDLRVGLVTNHNAIKDVSSKISKEKIVHKLHLMHTSLMQDFGIDKPRIALLGLNPHAG
                     DGGVIGTEEQTIILPAVQEAKNKGIMAFGPYPADGFFASCNYKKFDAILAMYHDQGLI
                     PFKYIAAEDGTNYTAGLDVVRTSPDHGTAEDIAGKGIADETSFRKAIYTAIDIFNQRA
                     NFAEMRENPLVKRAVLHDERI"
     gene            complement(28276..29748)
                     /locus_tag="PLL59_02880"
     CDS             complement(28276..29748)
                     /locus_tag="PLL59_02880"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HMY22778.1"
                     /translation="MLQISAKPAKRAILVLFTKVNLNILPFFDIEEKKAIQQKINDGV
                     TLFSFNKQQKTYILSSIEIDAKIEEDKQHERLRILGNSIFKVCKENKEQDISLEVLGD
                     IHQDSLAAILLGINLSNYEFLKYRTKPKGFTLTVHIDPILISETIVSEINLLTESVRI
                     TKDLVNEPVSFLTATQYSKEIQKLGEAAGFSVTVLDQKEIEAERMGGVLAVNAGSSAP
                     ATFNILEYKPAKHKNEQALVLVGKGVVFDTGGLSLKPTRNSMDMMKSDMAGSAAVVGA
                     MHAIATAKLPYYVIGLIPAVENRPGYEAICPSDVITMYDGTTVEVLDTDAEGRLILAD
                     ALHYAKRFKPELVLDFATLTGAAMRAIGKEASVIMGTAEKSIKKKLKKAANCTGERTV
                     EFPLWDEYTDYMKSDIADLKNLGPMDAGAITAGVFLKHFTDYPWMHFDIAGPAYVSAP
                     YNYRGKWATAYGVRLLFKFVQNLCNDEQPKPEKKSRNNAG"
     gene            29828..30616
                     /gene="rsmA"
                     /locus_tag="PLL59_02885"
     CDS             29828..30616
                     /gene="rsmA"
                     /locus_tag="PLL59_02885"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HMY22779.1"
                     /translation="MQKQRFVTALKSYGQHFLTDKLVLENIIAVIKKYHIDEDVIEIG
                     PGTGALTTHLAPEFSSLRCIEVDKRCIEYLQTTLLKTHQHVQIIDADFLKLDLPTILQ
                     KPTAVVGNFPYNISSQIVFSVLDNYKQIPFMVGMFQKEMAERIASPHGSKEYGVISVF
                     AQLLYDVKLEFDIAPQSFAPPPKVMSSVLSMKRKENIQLDFDFKLFKNIVKSGFNQRR
                     KMLRNGLSGIVAKDMLQHEFFQQRAEQLSVQDFIALTKMIEQSK"
     gene            30613..31539
                     /locus_tag="PLL59_02890"
     CDS             30613..31539
                     /locus_tag="PLL59_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMY22780.1"
                     /translation="MRKVLITAAIDSYIQLRFEALGFCVDVYEEIQRNELLEIIAAYE
                     VLIITTYTKVDKELLDIATNLKIVGRVGSGMENVDVIYARQKNISCVNSPEGNANAVG
                     EHSLAMLLALCNKLYKANNELKKGIFLREENRGFELDGKTIGIIGFGHTGQAFAKKLR
                     GFDVQILVYDKYQKTATNTAQHVSLQEIQKQCDVISFHVPYNAETHHYCNAEFLSKCV
                     RKPVIINTSRGAVVHTLDIKMALENKKINGFCIDVFEDEPISKNVINSKETYQDLLAF
                     DNVVATPHIAGWTIESKYKLAKILMDKIEECL"
     gene            complement(31552..32916)
                     /locus_tag="PLL59_02895"
     CDS             complement(31552..32916)
                     /locus_tag="PLL59_02895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMY22781.1"
                     /translation="MKTPDVTFLSLPKKLWYLLLVAFIVRVLIFLRFEPWQADVIKNQ
                     LLIFDSLGYHNLALCIKNEYSFCGDAFRTPAYPFFVAIVYAIFGEQPYYALIVQIFLN
                     LLSIVLMYKIGKLIFSETAGFIAALLFSLDLHHTIFIYQILTETIYTTVFLTGLWYYI
                     KAIKTNKLNYFIFTGLIYGISTLIRPISQYYIAGVLLFTLMWYFQKAWKNGWKAAIII
                     GIAYFIMIAPWCMRNYKEYNHFALSNIKGYNLLFWNASYYESKRLKLPIDTVNAHFKT
                     ELLKMGWRPDANPFEKEKYDGELASKILKANFPDYLKTHLIGTVKIHLSTGTHILTEV
                     LHIPAKTFSEEEKYTNGVFALIKKFFLEKTIYEIILGLFVALFLLIVYVFAAIGIWRM
                     LKEKQYLLMLFILGSAGYFTLISGIISYARYRLPSIPFYILLASAGIAWWLEKRKTSK
                     KESY"
     gene            33057..33530
                     /gene="greA"
                     /locus_tag="PLL59_02900"
     CDS             33057..33530
                     /gene="greA"
                     /locus_tag="PLL59_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HMY22782.1"
                     /translation="MSEVVYVSEEGLAKMKEELAQLIAVERPNASKAIAEAREKGDLS
                     ENAEYDAAKEAQGLLELRITKLQGDIGNARVLDASKIDTSKVSVLSKVKVLNTKLKKE
                     FTYHLVSEKEADLKQLKISVKSPIGAALLGKTKGEKITIQIPAGAMELQVLDISI"
     gene            33553..33948
                     /locus_tag="PLL59_02905"
     CDS             33553..33948
                     /locus_tag="PLL59_02905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HMY22783.1"
                     /translation="MASIFTKIIKGEIPCYKIAEDEHHFAFLDIRPLHIGHTLVIPKK
                     EIDYIFDLEDAELQNLIVFAKKIAVALKKSIACERIGIAVIGLEVPHAHIHLSPINSI
                     HDLDFKNAKEISKDTMLEIAEKIKANLDL"
     gene            complement(33957..34391)
                     /locus_tag="PLL59_02910"
     CDS             complement(33957..34391)
                     /locus_tag="PLL59_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22784.1"
                     /translation="MKKILLLSLLFLCLLSFGKCHKKKKCCKKKTNTATAVSKINPQE
                     VWMQYDETKCENPWHFNWFQKPTEEQLRSAVKGHLRGQEIAVLEIQSSFNKDIISCDA
                     CHCTNGRHYYVRINKSEIEKLKALKFFEIKEIPKFENIDTTK"
     gene            complement(34433..35329)
                     /locus_tag="PLL59_02915"
     CDS             complement(34433..35329)
                     /locus_tag="PLL59_02915"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HMY22785.1"
                     /translation="MIRPSFLKAGDTIGITCPARKITLAEIQFAIDIFKSWDLNVHIG
                     NTVGKEDNQYAGTDAERRDDLQRMINDTSIKAIIAARGGYGTVRIMDDLDYRALMDSP
                     KWIVGFSDITFLHTLLSCNIGMESLHATMPISFPNNTAAAIQSLKDALFGNSLQYTFD
                     KHSLNRNGIMQGEIVGGNLSILYANLGTKTILDTNKKILFLEDLDEYLYHIDRMMVAL
                     KRAGKLRHLAGLVVGGLSDMKDNTVPYGKCAEEIIYEHVAEFEYPVCFNFPCGHIADN
                     RAVFIGKQATISVADKCSFIQL"
     gene            35476..>36459
                     /gene="metG"
                     /locus_tag="PLL59_02920"
     CDS             35476..>36459
                     /gene="metG"
                     /locus_tag="PLL59_02920"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HMY22786.1"
                     /translation="MSTPKRYTVTAALPYANGPLHIGHLAGAYIAADIYTRYLRQQGN
                     DVIFVCGSDEHGAAITIKAKKENTTPKAIVDKYHAIIRKSFEDFGIAFDIYHRTSEQI
                     HYDTSQDFFRILNDKNAFEIIESEQYYDAEAKQFLADRYILGTCPTCANENAYGDQCE
                     KCGSSLSPTDLINPRSTLTNSTPILKTTKHWFLKLNEQQHDIETWLETKKDIWKNHVY
                     GQCKSWLNEGLKPRAMTRDLDWGIPVPQEIEGSEGKVLYVWLDAPIGYISATKQLFLE
                     KAQQENIFSYNQREFVDVSADDWKKYWQSEDTTLVHFIGKDNIVFHCITFPA"
BASE COUNT        12270 a         6233 c         6432 g        11524 t
ORIGIN      
        1 acatcaggtg attgccatta cacacttacc acaagtggcc gcaagagcga atcgacattt
       61 ctttgtatat aaaaatcatg agaaagaaaa aactttcaca gagattaaaa cattacaagg
      121 caatgaaaga ttacatgaaa ttgcagtaat gcttagtggc aataatccgc cgaaagaagc
      181 attggaaaat gcgaagcgaa tgttgaatta agatttctct ttaaaaaaga ttacccaaaa
      241 aatacataac aaactacgat actgagtatt atactcacca actcagcaaa taaactggtg
      301 gcaacaacat agcgtgtttt ttttatcgca actgcaccaa aataaaccgc caatacataa
      361 aaggtagtgt cgtgactgcc attaaaaatg cccgctaatc taccagcaaa actatctgca
      421 cccagtccac cttctgtaac tggttttatc aaatctacca tcaagccacg cgcaccactt
      481 ccgctaaacg gtttcataat ggctactggt aatgcatcta caaattgtgt gtcaaaacct
      541 aaactattaa aacaataggc aattccatgt gtgatgattt ccatgccacc acaagctctg
      601 aaaataccga tgcctactaa cattgctact aaatatggaa taatttttat cgtgatgtca
      661 aagccttctt ttgcaccatc aataaacgct tcatatacat ttactttttt aaaaaatgca
      721 aaagctataa atgtaatgat gactgttaat ataattaaac cacctaaaac attgctgact
      781 atttccattt gctctttcgg cattgtgctg aataaatacg tcaaaccagc aataaaacca
      841 actattccaa gcacccaagc ggtcagcaca aaatcccatt ttattttttg tatgaacgta
      901 cataatatca aaccagcaat cattccgcca tatgtagcaa ttaacaaagg cagaaatata
      961 tctgtaggat tggcagcgcc agcagtagca cgaaatgcga tgatgcttac tggaataatc
     1021 gtaaacccag cagtattcaa tgtcaaaaac ataatcattg agttcgatgc ctgttctttg
     1081 tttggattga tttcttgtaa ttctttcatc gccttcaaac ctaatggcgt tgcggcatta
     1141 tctaagccaa gcatattggc agaatagttc attaatatgc ttcccatggc aggatggtct
     1201 tttggtattt ctggaaatag acgactaaaa aatggactga ttagtttaga gaaaatattg
     1261 atggcgcctc cattttctcc aactttcatt atgcctaacc aaaagcacat gacgcctgtc
     1321 aatcccaaag aaatttcgaa tgcagttttg gaagaatcag tgatggcatt gatgcttgct
     1381 ggcataactg tcatgtcgcc gaaaatcaac atttttacca aaataataat aaacgacaac
     1441 acgaaaaata aaatccaaat ataattaagt atcatacgaa tgattttgta atcgaatgta
     1501 caaatttaat ttgctaatta gttaattagc taattaatca ataaatttgt tttgtgagca
     1561 agaaatacga attaccagaa attgtaaata gaaaagcaaa attcaattat gaatttttag
     1621 agacgtataa ggctggtatg gtgctaactg gcacagaaat aaaatctgtt aaagaagcta
     1681 aggtaaatat tggcgatagc ttttgttatt tcagacaagg cgaattgtgg ttaaagaatt
     1741 tacatatttc tgagtataaa aatggaagct atgccaatca cgaacccttg cgtttacgta
     1801 aacttctttt gaccaaaaaa gaaataaaaa aattagaagg aaaaataaaa gagaaaggat
     1861 tgaccattat tcctttcaag ctttttttta atgaacgtgg cttggcaaaa attgaaatag
     1921 cattggcacg cggtaaaaaa tccttcgaca aaagagaaag cattaaagag cgcgatacca
     1981 aaaaagaaat acaacgcgtg caaaaatcaa tgcgataaga tagctatcta ttcttgtgaa
     2041 atttgatttt aaatactaaa tttataatat catgatttct gcttttgtat ttttagacgc
     2101 agtatcgcca ataatattag caattcctgc tttgctatta atagtattaa ttgcagtaat
     2161 aacatttggc attctttact tgattgcgta tattcgaaaa agaaaaaata aacaatgaaa
     2221 caaaacctaa ttactcttac cttgtttctt cttgttcatc tctcttttgc cgatgttccg
     2281 attgatgaca atgaaggcac tttaagtatg tattcaaatg ccaacttttt acctttaata
     2341 cttggtgttg ctactgcaat agctattttt gtttttatgt atgtttataa aaataaagtc
     2401 aatgaaaaaa aataaactca ttattctctt cagcttatta gtgcttaaac tctctgctgt
     2461 agctgatatt gcattgccta aaatagaaaa ccttcaagaa acaaaatact attttgccaa
     2521 tgtggactcc tttgcaaatt atacatttta tgttaaaaag aatgcaaatg ataaaacata
     2581 ccgcatcaaa caatccagtg catttttgct aaaagataat agtaaaatag gcgaggagca
     2641 aattgaagtt tgggcagtgc gaaatagtga taaacagaaa tcaaatacat ttgtcttaaa
     2701 gcgcgttaaa ccagcgattg aacatggtgg caccacagca catattgcta ttatgctttc
     2761 tttcgataaa aaaaataatc tgaattacaa acaagtagta atgaagccag agtgttataa
     2821 gcgtaattat attgttccaa taattccgtt tacatttaca ccaacgaata gcctattatt
     2881 gcttgtttca ataatttcaa tgctgatttt attctcttta gcaatgtata aaccagtttt
     2941 tagacgcatg aagtatgctt aaatatttag cattaactat tgctatagaa attccaatct
     3001 attttttatt cgacagaaat aaaatagtgt atagtacatt tgttttgttt ttagcaaatt
     3061 gttttacatg gccaattgtc aatattttat ttcataatac caatattcat ttgctgattt
     3121 tagaaagtgg tgttaccatc gtagaagcca ttattatcaa tattttttta caagataaat
     3181 tttcaaaatc tttattaatt tcattcatac aaaatagtat taccacattt attggcattt
     3241 ggataaataa catacagcta tgaacaataa atataaaaca gctactttgt ttggaatggc
     3301 gcatggcttg aacgatttta ttgcaggtta tttattggct aatttgtcat cacattcatc
     3361 agatattttt accaattctt tagcctactt gatgtacagc gtgatcgcat ttggcggaca
     3421 attgccagca ggaattattg tcgataaata taaacgaatc aagccgattg ctagtatatg
     3481 cttattgctt ttgattttgt caatatctgt ttcctatgca agtttattta tggccatatt
     3541 ttttagtgct attacttcag catttattca tgtgtgtggc gcagcagcct gttatgtttc
     3601 tgataataaa cattatacac ttgctggtat ttttacatca ccaggtgtga taggtttaat
     3661 atgtggtggc atattaggaa gtattgattt caactacttc tatttgttta tcttgccatt
     3721 ggtcgtattg ttgttatggt taatgtatgt agaaattcct tcctacgaaa ataaaaaagt
     3781 gaatgcacac tcatcagata caatacttga cacacacgat atggtcatgc tcttattgtt
     3841 aatgacaata gccgtacgtt cattattttg gaatgtcgta cacatgatga gttttaatca
     3901 acaaacgtgg ctcttagtac taggtttttc agctggtgcc ggaaaattat caggagcaat
     3961 aatcgccgat agagttgggt ggaaaaaatt tatgtatgta agtattattg gtgctgtcat
     4021 ttgcctaaat gtcgataaat ccaatattgt tctatttggt attggtgttt tctttttgca
     4081 aagtgctgtt cctattacct tggttttagt gcaacaatat atgaaacaaa gtccagctac
     4141 tgcggccgga ctttgtttag gtgtattaat tttatttgca ggattaccat cgtatttcca
     4201 tgcatttagg caattacaga cacatcaaca tattaatgtt gtgctaagtg ccctgttttt
     4261 cctatctaat ttctgtttcc ttttgatgta ccagcgcaaa gctaaatatc agtaacagaa
     4321 gtttccatgt cagcttgata gagcaaaata ctgatgcaac taaagaatac gacaatggca
     4381 gatgcaaaca tcagtccacc gtcgcctaac acatcaaatt cattggtgat gatgtgtact
     4441 acaattgcgc cgagcattaa aaccatgcca agcaatgcac cacgccatgc catttttgga
     4501 atcaacaaca agataatggc aagaatttct aaacctgcaa cactaaatct tccagcaggt
     4561 tccatgttga tgcgttggaa aatatgcact gcggccggag cagcagtaag tttcataaat
     4621 gcggaatagg aaaatattcc agcggcaata attcgggttc ctatgtctat caattttgta
     4681 ccatttttca tagatcatag attttattgc atataaattg cacttgtttt gtcgatttgt
     4741 caagttaaat atgccatttg ctggaaagaa tgagtattcg gtcgtttttt acaattaatt
     4801 ggtttgtttt ttattctaaa aaaaagaaag gcgatgcact tgcatcgcct ttctataata
     4861 ttttatagtg aaaatattat tcgataatgc cttctgttaa aacagaaaca ttatttttta
     4921 atacttcaac tataccactt ttaaggttaa attcttgttt tccagcagca gattttatgc
     4981 gaaccgtacc ttttaccaag ctcgcaatca ttggtgcgtg attatttaag atttcaaact
     5041 ttccattgct gccacaaagc tgtacagaat ctacttctcc tgaaaataca tttaattctg
     5101 gcgtcagtat gttaagtgtc atataatgat gtgtttaaga tttggtgagc gaaataacag
     5161 ttttgatgct gttattaaac ccatgaatac tttgttttta ttatttagct tcagctaata
     5221 attttttacc tttttcgatg gcatcttcga tagtacctac taagttgaat gctgcttctg
     5281 gatattcatc tacttgacca tccataatca tttcaaatcc tttcaatgta tcttcaatgg
     5341 aaacgaatac acctttcaag ccagtaaact gttctgcaac gtggaaaggt tgagataaga
     5401 aacgttgaac acggcgtgcg cggtgtacta ctaatttatc ttcttcagac aactcgtcca
     5461 tacctaagat ggcgatgata tcttgtaatt ctttataacg ttgtaagata gattttacgc
     5521 gttgtgcaac attgtagtgt ctttcgccaa ttacttgagg gtctaagata cgtgatgttg
     5581 agtctaatgg atctaccgca ggataaatac ctaactctgc aattttacgg ctcaataccg
     5641 ttgttgcatc taagtgagca aatgtagttg caggagcagg gtcggttaag tcatctgcag
     5701 gtacgtaaac agcttgtact gatgtaatgg atccagattt agttgaggtg atacgctctt
     5761 gcatcaaacc catttctgta gataatgttg gttggtaacc taccgctgaa ggcatacgac
     5821 ctaataaagc agatacttca gaaccagctt gtgtgaaacg gaagatatta tcaacgaaga
     5881 ataagatgtc ttttccttta ccgtcgccat cgccatcacg gaattgctct gcaatagtta
     5941 aaccagataa agccacacga gcacgagcac ctggaggctc attcatctgt ccgaaaacta
     6001 aggtcgcttt tgattctaat aatgctgttt tatctactgc agctaagtcc caatctcctt
     6061 tttccataga gtgtttgaaa gcttcgccat atttaattac atctgattct aagaactcac
     6121 gtaataagtc attaccttca cgagtacgct cacctacacc agcaaatact gacaaaccac
     6181 cgtgaccttt tgcgatattg tttaccaatt ccataattaa taccgtctta cctacaccag
     6241 caccaccgaa taaaccgatt ttaccacctt ttgcataagg ctcaattaag tcgataactt
     6301 tgatacctgt aaatagtact tcttttgaag tggctaagtc ttcatattta ggtggttttc
     6361 tgtggataga ataaccgcct tctttgctca cttcaccaat accatcgata gctgtaccga
     6421 taacattgaa taagcggcct ttaatcgctt cgccggcagg catcgtgatt tgatttccag
     6481 tatcggtaac tgcagcaccg cgagttaaac cttctgttgc atccatagct acacaacgta
     6541 cgctgtcttc tcctaaatgt tgttgacatt ccaacaccac ttcagagcca tcgtcttttt
     6601 ttactattaa tgcgtttaag atatttggca gtgaagcacc ttcgccgccg aacgtaacgt
     6661 ccaccaccgg accgataatt tgggtaatct taccaacgtt tgccataata taatgagtat
     6721 atttgattta aaatgcggtg caaaaatacg gctaagtagc tgtttttcaa aatctacctt
     6781 ttaaagttgt caacatataa agatttggta atttttaatg ataatttcgt ggaaatctta
     6841 ttgtaattgt tagtaaatca gtacatttaa tcatgttttt tcctaaatgg cgaccttttt
     6901 tgttggtttt ggtattttcc aaatccttta tttcattttc acatcaagat tctataaaat
     6961 gcgctacgga attcaatttt attaataaaa ccattgataa tcagtatgta atgtctggtc
     7021 ggcttgcatt tgaccagcga tttagacaat ggcaagaaaa taatattcaa aatagaaatt
     7081 cctcgatatc atccttaatt acaattcctg ttgttgtaca tgtcatatat aatacagcaa
     7141 ccgaaaatat ttcagatgcg caaattcaat ctcaaattga tattcttaat aaggatttta
     7201 gaaatttgaa tacagataaa ttggcatcga cacatgcatt ttataatttg tctggcgatg
     7261 caggcataga attttgtctg gctaaagaag accccattgg caatgcagtg tctggcatta
     7321 cacgtactta tacaagccta caagaatttg gcaacgaaag caatatgaaa tatgctgctt
     7381 taggaggcgt tgatgcatgg gatacaaagc gatatttgaa tatttgggtt tgcaatttag
     7441 gcaagcaatt gcttggattt gcgacctatc cgacggcatt tgaatatgcg ccgaacgaag
     7501 atggcgtagt attgaatttt actgcttttg gtataatggg aacagctaaa gcaccgtaca
     7561 ataatggtag aacagctacg catgaaattg gtcattggtt gagccttaga catatttggg
     7621 gcgatgacaa caatagttgc gacgacgacg acggaattga cgatacgcca aatcaatttg
     7681 gtgctacggc aggatgtcca agcggcgttc ttgttgatgg atgcacagtt tcctccacag
     7741 gtgtaatgta tcaaaactat atggattatt ctgatgatgc atgtttggta atgtttacaa
     7801 aacaacaaat tgcaaaaatg agatttgtat ttgatgaaat taggactgaa atgttgagtt
     7861 cgccaatttg ctcgcgaacg actggcattg cgaatcaagt gcattcaaaa gtgaatattt
     7921 atcctaatcc aatacagaat tatttgtcga ttgaaggatt gccaaaaact agaaacaaaa
     7981 tgtatagtgt agaattattc aatatgattg gcgagctcgt tttttctact gcaattcctg
     8041 ctgatgatac cttgattgaa atgacagaat tgccaatagg gacttatgtt ttaaagattt
     8101 ataacaatga ttttactact attcataaag tgacagtagt gaaatagttt tttcattacc
     8161 aactctctat ttctgtttta aaattttcca ttaatttctt tttagcgcga tgtagttgta
     8221 ctttaattgt gccgacagaa acacctaatt cttcggccat ttcttcatag ctgtattctt
     8281 tgaaatagcg caaagtaagt acacgcgaaa atacagtatc aagtgtgttt actgctgctt
     8341 gtaatttgtc gatgcgctgc gcctttatca tgtcatattc aggtgtgctg ttggtactat
     8401 attttagatg gtctgtaatg ttttttgctt catcatctaa agatattttt ggtacatttt
     8461 ttttgcgcaa aaaatcgatg caagtgtttg aactaatttt atagagccat gtagaaaaag
     8521 cataatcagc agaatattga tcaatatggt caaaggcttt ggaaaatgcc acaatcgtta
     8581 aatcttcagc gtcttcaggc gatttcacaa tttttaaaat gagatgatag acagactttt
     8641 gatatttctc cattaaagca gtaaaagctt tttgatggcc atttttagcg gcgagcaaca
     8701 agttttcttc aatattattg gcgtcgttta tttccaatct cttgtagatt taatgaaact
     8761 aataattcca aatacagtag tgaagatact atataaaata tcattaatgg cagaaaaata
     8821 ccatcggtga ttaagttgaa attttttgtg caattgaaaa ttaaacattg taataatgat
     8881 aaaatagcct attaatatgc caagaaaata aagtgaaaaa acagagcatg aaataattat
     8941 gattaagcat aaaaagtaca tcaaaaattt agaaactaga taggcaccta ataccagttg
     9001 atgagcatat ttatataaat gtccactttc gtaatgtcgt attttttgtc gataccaatc
     9061 ttttaaagtt tgcttagctt cacttattgt gtagctgtca agcgataagg aaacgctagt
     9121 gtttcctgcc gttgacaaag attgtatcgc caaatcatca tcaccagaag caatagcaaa
     9181 ttcttgtgtt gaccaagatt tacttttcaa taatttggca tcataaatta catttctgcc
     9241 tacgctcata tatggcattc ctaataacgc aaaaccacaa tattgccacg cagtttgtgc
     9301 tgtttcgtat tcgataatgc cgtttaagaa agtatctgtt ttttgatatg gtgaaatgcc
     9361 aagtacgatt ttatgtgtgt cacttatgcc atttaccatc gaagtaatcc acttatttga
     9421 aagcggtttg caatcagcat ctgtcaacac aatcaaagaa ttttttgctt gtgaaatacc
     9481 tttttgtata gcatattttt ttcctattcc tatcttctca tttttatcaa ttttaattat
     9541 cgttaatcgc tcgtgctgta gctgtatagg aacatcagaa ccatcgtcaa ctaatataat
     9601 ctcaaagtca ggatattgtt gatggaatat aaaaggtaaa tttttggtta gattttctgt
     9661 ttcatttctt gcacaaatca ctatgcttac ggaaggaaat tgctgcaaag gtgcaatgat
     9721 tttatttttc tttttaatga gtgcgcacag tagtatgcat tggtaaatta cctgaaatag
     9781 cacgcagcaa ataaaaaaaa gcatcataaa tttcacttta taaaagtaag gtttttgtcg
     9841 tacttttgtt ttttaatttt tgctttttga aatacgaaat attacataca gacaaacatt
     9901 cttcagcaag agctggaaaa ataagtacag cgcatggtga cattgaaacg ccaatattta
     9961 tgcctgttgg cacacaaggc acggttaagg cggtacattt tactgaatta gaaacagata
    10021 taaaagcgca aattatatta ggcaatacct atcatttgta cttgcgtcct ggtttagaaa
    10081 ttttacaaaa agctggcggc ttacatgctt ttaatacttg gcaaaagcca atattgacag
    10141 acagtggagg ctatcaagta tattctttgt cgcatcgtag aaagctcaat gaacaaggcg
    10201 ttaccttcca atcgcatatt gatggcagtc gacatttatt tacaccagaa aatgtaatgg
    10261 atacacaacg tatcataggt gccgatttta taatggcatt tgatgaatgc actccttatc
    10321 cttgcgaata taagtatgca aaaaaatctt taaaaattac acatgattgg ttgcaacgtt
    10381 gtattcatcg gtttgataca acgaagccac tttacggtta cgagcaaact ttaattccaa
    10441 ttgtacaagg ttcagtatat acggatttac gtaaggaatc agcagcattt attgcagcac
    10501 aaaacagacc ggcaaatgcg attggcgggc tcgccgtagg cgagcccgca gagatgatgt
    10561 acgaaatgac agaaattgta tgcaacattt taccaaaaga caaacctcgt tatttaatgg
    10621 gcgttggcac acctgccaat atcttagagt ctattgcact cggaattgat atgtttgact
    10681 gtgtaatgcc tactcgaaac gcaagacacg gtttgttgtt tactacagca ggaattatta
    10741 atattaaaaa tcaaaaatgg aaagatgact tctcgccaat cgatataaat gtgcctgctt
    10801 ctacgagcca aaaacactcc aaagcgtact tgcgacattt atttgttgtc gaagaatatt
    10861 tagcagcgca aatcgcaagt attcacaatc tttcttttta cttgtggttg gtaaatcaag
    10921 ctagagaaaa aataaaagaa ggtacattta ccacttggaa aaacgaaatg attgtaaaat
    10981 taagtactag attataaaac aaaacacaca attatattcc atttcttacc gcgaatgaaa
    11041 atactcgaca gatatatcat caaaaaattt ctcatcacat ttttctttgc aatatttttg
    11101 ttgagtgcca tcgcaatcgt gattgatatt actgaaaaga ttgacgaatt tatcgataac
    11161 caagcgcctt tacatgctat cgtcgtcgat tactatttga attttattcc gtggataggc
    11221 ttgatgctgg ctccgatttt tgtttttatt tcagtggtat attttacatc gagattaacc
    11281 gcaaacacag aagtcatttg tatgctaaat ggtggtgtta gcttttatcg attgttgttt
    11341 ccttatctat tcacggctgt tttattagca atgttatttg attattataa tcatgagctg
    11401 ctaccaaaaa ataacaaagt aaaaattaaa tttgaagaac aatacgcatc aaaaaaaaat
    11461 caacgctcag cagatatcaa ttatcatttt caaataggaa aagatacttt tatatacaca
    11521 caatctttcg atatcaacgc taatacaggc tttaaattca ccttagaaga aattaaaaat
    11581 aaagtacaac actataaatt cacaagcgaa aatatgtcgt gggacacaac tgcaaaaaaa
    11641 tggagaatgc gcaattggac atttcgaaaa agtttaggca atcgcgatag tataggaaaa
    11701 ggtatggact cacttatggc attgcctgtg accgcaaaag aatatgcaaa acagaattat
    11761 gatatcgaaa cgatgacgac aaaagaattg aaacaattta tcatcgacca aaaacataaa
    11821 ggcaatgaga atgtgaaaat ctatgaagtt gaaaaatttc gaagaacggc cattcctttt
    11881 tctaccatta ttttaacttt aattgctgta gcgatgacta caaaaaaagt acgcaatgga
    11941 atgggcttgt acattgttgc tggtatgctg ttgagtggtg catatattat cattcaacaa
    12001 ttttcaactg tatttgccac aaaaggcaat cttgatcctt taattgctgt gtggattcca
    12061 aatattattt ttggtattat cgcagcttta ttaataaaaa gagcgcctaa ataaaatgca
    12121 taattatctt ttgtatgctg ataatcacca atcttaggta atctttgtta tcaaattact
    12181 aaaacgcatc tttataagaa taagcacgta tttttgctat tcggaattaa tctaaataat
    12241 ttgacagata aaaaaaatat aacacaacta caaataggcg aaagtgcttt gataacagca
    12301 tttgcagatg gccaaattgc agctaagcta atggcaatgg gcttgctgcc aggaactact
    12361 ttggctttgt tacataaagc accgtttggt ggcgcatatt atatcaaaat caataatcat
    12421 tatttggcct tgcgtgtcaa tgaagctaaa aatgtactca taacttctgc ctcataatgt
    12481 ccgtccaaaa atataaaata gcactattag gcaatccaaa tgcaggtaaa tcttctttgt
    12541 ttaatcagct aaccggtttg cgccaaaaag taggaaattt tcccggcgtt actgttgagc
    12601 gcaaaagtgg tatcataaaa gataatgcta taaaccttga aatcattgat ttgccaggaa
    12661 catatagctt gtatccaact tctttagatg agcgtattgc cgtacacgca ttgattgaca
    12721 gcaagaacag agattttcct gacgctatta tttacatctt agatgttaca catttagaaa
    12781 gacacttatt gttgcttaca caaatttctg atttgcaatt tccgatagtt gttggtttaa
    12841 atatgaatga tattgcagat cagcaaaata tgtattgcga tgaaaaaata ttgtcgcaag
    12901 cattaggaat tgaagtcata aaaattaatg gtagaactgg tgaaaacatg gcactgttaa
    12961 aatcaaaagc aattaattta ttgaaacagc caattagcaa tcgccaagtt ttttatacaa
    13021 ttaatgaaca agaaaaaaat atagcaaaag atgtcgctct gcttttgcca gtgcgaaacg
    13081 aatatcatgc attgctatta gcacaccatg cgaaacgttc aacgttttta gtacaaaata
    13141 taaaacaaca attactgcaa attaatacaa agcatcaatt taatcctatc aataatcagc
    13201 tagatgaaat tatgcaacgt tacaacgcat ttgtaccgat tgtcaataaa gtattaaaac
    13261 gttcatttga taatgctaaa acactaacgg ataaaattga ccgtattgta actaataatt
    13321 attttggacc tattttattt ttcggtttaa tgttgtttgt ttttcaagcc atctttgcat
    13381 ggtctgcatt tccaatggat tggattgatt tgcaattcgc caatattgct gcatctttac
    13441 aagcatcctt accagataat ttatttacaa gattgttgac agaaggcatc attccaggta
    13501 taagcggtgt gcttgtattt gtgccacaaa tcacgatttt atttttaata attaccgtat
    13561 tagaagaagt gggttatatg gctcgtgctg tatatatgtt tgaccatatt atgcaaaagt
    13621 tcgggctaaa tggaagaagt attgtttcgc tcatttctgg tggcgcctgt gctattcctg
    13681 ccattatgag cacgcgaaca ataagtaatt ggaaggaacg tttgactacc ataatggtaa
    13741 caccattaat tagttgttca gcgagaatac ctgtttatgc agtattgata ggctttatcg
    13801 tgccgagcta taagcttgga atttttaatg cacaaggctt agtgtttatg gcgctttatt
    13861 taattggaat tgtatctgca ttaattgcaa gtttggtttt taaaaaagta ctaaaatcta
    13921 cagatgtttc cttcttgatg cttgagttgc ctgcatacag aaaaccacat tggaaaaatg
    13981 tattttttac attatatgaa aaagtaagtt cgtttgtttt tggcgctggt aagataatat
    14041 taatcgtgtc agtcatcctt tggtttttgg ctagctttgg acccagcact gccatgaaaa
    14101 ctgctgaaaa tactgccatt attgagtcat cattaatgca agctgatagc gcgacaacac
    14161 aattacatat tgcttcaaaa aaattagaag catcttatgc tggtcattta ggaaaatgtt
    14221 tagcgcctat tattcagcca ttaggattcg attggaaaat tggtattgca ctcattacgt
    14281 catttgcggc acgcgaagtt tttgtaggca ctatttcaac tttatattct atagggaaag
    14341 atgcggataa cttgacgata aaacagaaat tgcaacaaga aaaaaatgca gatggtcaac
    14401 ctgtattttc aatggccgta gctttatcgc tggtattgtt ttatgttttt gctatgcagt
    14461 gtatgagcac gctggcagca gtttatagag aaacaaaatc gtggaaatgg ccagcaatac
    14521 agttcgccta tatgacaggt acagcatatc ttgtttctct tttagtttat caattcctaa
    14581 aataaacaaa cgccagtttt ttttgttgct atctcaaatg tgtatgctat gaaaatatta
    14641 actacacttt gttgtatgtt gtttttaatt caatgcaata gatgtaatga aaataattcc
    14701 aaaaaaacta gaatacaaac gactatgaat actactgcaa actcatcatc gtcagcaatg
    14761 gataccgcaa ctttagctgg cggttgttat tggtgcttag aagcgatttt tcaacgattg
    14821 caaggtgtag aaaaggttac ttctggattt tgtggcggac aagtaaaaaa tccaagctat
    14881 aaagcggtgt gtactggaac gactggacat gcagaagtaa tacaacttac ttatgatagc
    14941 tctgtaatct catttgctga aatacttaaa gtattcttta ccatgcatga tccaacgaca
    15001 ttaaatcgac aaggaaacga tgtgggcaca caatatcgat cggctatatt ttatcataat
    15061 acaactcaaa aaaaggaagc ggaagaaatt attcatgcat taaatatggc atcagtttat
    15121 tcaacgccaa tcgttaccac agtagagcca tttgatgttt tttatattgc tgaagattac
    15181 catcaaaatt attacaacga caatagcaat gaaggatact gtcgatacgt gattcaacct
    15241 aaacttgaaa aatttgagaa agtattttct gataaattaa aaaaataaaa aatcaaacga
    15301 tctagctttc atcagattgc tcttgctcat cattacttgc cgatagattt ctttttggat
    15361 aattaaataa aatggagcta attacaggca agaaaaatac caataccgtt attattgaaa
    15421 cgattaaatc aaaatgttct gattcaatat atttaaatgt agtgctgtct tttaaataat
    15481 gtgcaggtat ggaaagcaat agctttacac ctaatattcc aatgacaata aatgccgctt
    15541 tctccaaaaa tggatatttt tccatcaact ttacaaacga ctgtgctaca aatcgcatgg
    15601 ctaatatgcc tataaatacg ccagtccaga ttaaatagat attgtcagta aatgcgactg
    15661 ctgcaaaaac attgtctata gaaaatgcca aatccatgat ctcgacaagt ataacagtag
    15721 accaaaattt tccgaataaa ccaagcgtat atttgtagat gaaactttct tctttattga
    15781 gtgtatcatc ttctggcttc ggtgtggctt ttgtcctaaa ataatcaatg aatagatata
    15841 ataggtatag tccgcctaag gcttttagcc accatatttt tacaagccaa gcggctagta
    15901 ccaaacaaat acctctaaat acataagcgc cgataatacc atatttcaaa gctttcttgc
    15961 gttgttcctt cggtagatcc atcaccattg ttgccaatac agcagcattg tccactgata
    16021 atagactctc aattaatatc aaatttaaaa tgattattag cgcttgttgt atagatatat
    16081 cttgaagtat tgtttccatt ttttttataa aatcaagcca caaggtcaaa aaaaaatctt
    16141 aacaaagttt aacttattga ataattaatg taaatttagt gtgaaaactg tcctattatg
    16201 aaaaaaatct tactgctatt cgcagcaatc tctattgctt ttttttcttt ctctcaaaca
    16261 aaatacttcc caacgccagg cgcttctgaa attcctggta gtaaaatcaa cggtaagtta
    16321 gatgacatta ctgacttctc tacttgtacg caagttccgt ttacgatgcc agatggtaca
    16381 aaattaatga ccgatattta tctgcctatt ttgcaagata gttttgttta taatgataca
    16441 ctgacattca gcttattgcc agatccattt ccgccaatta agatacctat ttatgctgtt
    16501 ttactaaaaa aaggccagca atatctaata tatgattcta tcaatggtgt gcctaatcct
    16561 aatccatatc aactacctct tatcttggag cgcacacctt atgataaaga tggcgctgta
    16621 gaaggtgcag ccatggcatt acttggttat gcaggtgcgg tgcaagacat gcgtggacga
    16681 tacacttcac aaggtgcata tctgcctttg tattctgata gctggaaaaa atggccttat
    16741 cacaattaca aacatgtttt agatatcaca gatgccagcg atccaagaaa tggcaacaat
    16801 catgaagatg gttataatac gattgaattt attaaaaatc aattggttcg taaatatgat
    16861 ttgaatagag atggcaattt tgagactaca gatttattat acaatggctc aattggtagc
    16921 tttggtgcat cagcattggg ttacaatcaa ttgcaggccg ccgcagcaca taaaattgat
    16981 ccttcacaac cgggtttaaa atcaatgttt ccaatagtag ggccattgga gttttataaa
    17041 agtacaggtt atcaaaatgg ctgccttaga gatggcttag taacaggttg gttgcgtgga
    17101 caaattaaag atacgcgtga cgatttatgg aatatagaca gtggcttgaa cgatagtatt
    17161 cattcttcaa aagattataa tacagtagat aaatttgaag catctaataa agcaattgac
    17221 cactttgtta ctgtgcaata cgaaaatagt ccagctggct attatccaaa ttctattgga
    17281 agaagtgaca tggacgccag taaagcacca gtagatatta atggagaagg cgcaaaaaat
    17341 ggcgcctatt caagatataa aaatatcgaa gtgccaactt ttcatgtagc aggatggtgg
    17401 gatatttttg tagatggttc tttagaaaca cacagattta tccaagataa tataacacag
    17461 aaaaagaatc tccaaaaaat aataatcggt ccatgggcac atcaaaccat tgcctcaaca
    17521 aaaacaggcg ataaagtata tccaaaaaat gtgacagata tcaccaaaat agatattacc
    17581 aatatcagag gttttgaaga tttagatatt gctgaattag caaaatcaga attgataagc
    17641 tggttcagat ataatttgaa tgataatggt tacaataaag taggcgaccc gaaagtatta
    17701 atttccaaaa caagtgaatg gcaggcacca cttcctcaat taccacaatt agaagtgcgt
    17761 ttcccttcgg aagattataa gattcaattt gcggatttat tgaacttcat tcttggacaa
    17821 gataatttga aacaagtacc agtagaagtt cgattaggtg gaagcagtgg tttagttatt
    17881 ccgtttaaaa ttgatttgcc agatatggga ccacttatcg agggctttgg ttcgaatggc
    17941 gcatttactg gaattcctaa atatgactat acgacaatac cgagtattcg ctattatgtt
    18001 acaggtgctt caagcgatga tggcacggtg tcgtatgcag gaaattattg gatgggctct
    18061 gaaacatttc caccgccaag tgtgcaatgg cattcaatgt ttttgcatca aaatggaaaa
    18121 ttaaatttta caaaaccaac aagcgatgaa ggttatagaa cttatgttca taatcctgat
    18181 gatccaatta ttacagttgg tggtggcaat atgctagaac gcacgccaca aggcgatcga
    18241 gacgcacaag gccaaatgaa tttggcagat acaaactttg caaaatttac tatggacaga
    18301 gaaggcgtat tgaaattcga gactgatgtg ttgcaggatt ctttatgtat tatcggcttt
    18361 ccaaaatata aattatatgc aaaatcaaat cctggaaata caccatcggg cccaaccgat
    18421 tgcgatttct ttgtccgtat tttagatgta tatccagatg gaaaagagtt ttttgttgtg
    18481 gaaggtgctg taaatgctcg tgcgcgcgaa tatgctaaat caatcgccgt tggtgcagaa
    18541 aatgacaatg caccatttag caatatcgat atcggtaaaa tatatgaata ttatttccaa
    18601 ggttatccga tagcatatac ctttgcacaa ggacacaaaa ttaaagtgct gatatctagt
    18661 agcaattatc ctcgtttcca agcttgcgca aacgtgccta tcacaccaaa tgacttcttt
    18721 aggcgaaaac cagcagatgg cagaacatat atgtttaatg gcgttgagta tgcacctcgt
    18781 atttctgttc aacgcatcgc attttctgat cagtatccaa cacaaattga attgcctgtc
    18841 tttggaaatt catctttagt aacagcgttg aaaaataata gcaccaaacc aacatgggac
    18901 attaatttat ttccaaatcc aagtaatggc gcattctctg tttttattag taaaaatgga
    18961 aaatacatcg caaatgtgta taatatgatt ggtgagaaaa tactttcgag agaaatagta
    19021 gaacatcaat cattcaacat gaaagacttg gcgaaagggc aatatttact ggagattttt
    19081 gatgtaaaaa atactgacca aaaacaaaca aagtcattta ctataaacta agaaatttac
    19141 gaaccaataa aatctaagca atgcacacta cgggaaattt tttcaaattt tttacagtat
    19201 tattttgtct attttcaaac atatatttat acgcgcaaga aattacgatt agcggacaag
    19261 ttattgataa aaataccaag gaaccactta ttggcgcaaa catacttgtt aaaggaacaa
    19321 ctattggtgc aacaaccgac attgatggca acttcatcat tcatggagat attagacagc
    19381 cagttgtaat tggtagctac ctaggatatg caacgcaaga aataacagta acggaaggaa
    19441 ataaaaaaat agtaatagcc ttagcgccac aagaaatacg catgagtaat gatgtcgtaa
    19501 tttccgcatc gcgcttgagt gagcgcattc aaacttcgcc atcgtctatt caaaaactta
    19561 atgcaaaaca aattcaatct gttgcatctg gtaattttta tcaaagcttg ggcaacttaa
    19621 aagaagtgga cattacaaca acgagtatgg gctttcaagt gtttaataca cgtggcttca
    19681 atactactgc gccagttcgt attgtgcagt ttatagatgg tatggataat caagcaccag
    19741 gcttaaattt tccagtcgga aatttagtag gcgcaaacga tttagactta gaaagcgtag
    19801 aaataatttc aggcgcttct tcagcattat atggagctaa cgcatttcaa ggtattgtaa
    19861 gcatgcaaac aaaaaatcca ttcgactata aaaatttgca agtgaaaata aaaggcggta
    19921 atcgccaact tatcgatgca caagttagat atgccaacgc tttcggcaaa aagaaatatg
    19981 gaagagatgt gtttggcttt aaagtaagtg gttcttattt tcgcgctaaa gactggattg
    20041 ctgatgatac gattgccaat gtatatggag acataggtac tgatgtgaat gtaagtacag
    20101 tcgttcgtaa actgcaatac aatgaagaca cagcaattgc gcgacagttc agagcattga
    20161 atgcctattt agattttttt ccaaacgcat ttcctggaac tattcatgtg caagcacctg
    20221 gctatagaga aaaagacttg actgaccagc aaacagaaag catcaaagca agtgcagcat
    20281 tatatgcaag accaatcaaa gatatgcaaa tcgagtatca gtataaattt ggaagaggta
    20341 gcgctgtgta tcaaggcgca aacagataca acattaaaaa tattttattc cagcaacata
    20401 aagtccaatt ggattataaa ggcttgtcgg taaaatatta cacaacacaa gaaaatgctg
    20461 gtaaatctta tgatatggta tttagcgcca tcaatttatc caagattgga attacacgct
    20521 atgtgtcaaa ttttgtaaaa gcatattttt ccaaactatc cgattttaca aatactttca
    20581 aagatgatcc agaagatgca gacgtgcaga catctcgcga atatgcctta caagaagcac
    20641 taaataatgc gtatttacaa ccaggaactt tctcctttga ttctgcctat aatatcattg
    20701 ttgatgatgc caatttggtg acaggctcaa aattccaaga taaatcatca ttgcatcatg
    20761 tggaggctaa ttacaatcat tcttttaaac acgacatcaa tttaattgta ggcacttcct
    20821 atcgaatgta tattcctcgt tcttatggtt ctatctttta tgatacctta gccaatagag
    20881 cagatacgct tgctgatggt tcgcctgatt tagatgcaaa atttataaat ctaaacactt
    20941 gggaagttgg agcttacaca caaattagtg ctgatttatt taaaaaacat ctgaaattag
    21001 ttggttctgc acgcatcgat aaagcggaga attttaaagt acaattttcg cctagaatct
    21061 cattgattgg tacttataaa gaccatactt ttaggttctc gtatcagcag gcgtttcgct
    21121 cgccaaccct acaaaatcaa tacatttcac tcgaccttgg tgctatacaa ttactcggca
    21181 acttgcgtgg cgctgaaggg tatgacttcc aatcggtcaa agactttaga agcaattacg
    21241 acgttacctt agaggttgac ccaactttat taaaaccaat aaaactaaat ccgataaaac
    21301 cagaacaagt aaactcttgg gaattgggtt atcgcggtgt attcgctaga aaattgtatg
    21361 tagatgtgtc ggcttatttc aatcgatatt ttaattttat tggcgacttg cgtttttaca
    21421 tgcctgaaaa taaagacatt gttgttggta cagaagcagg tgcagatgct atgttgacag
    21481 gtgcctataa cttaattcaa atgccaacca atgccaagca aaaagtagat gcttttggtg
    21541 cttctttagg tttaaattat tatatctgga aatcattgat aggctcattt aattatactt
    21601 attctggtat taacaccaag aatttgaaag atccaattat tcctggtttt aatacaccta
    21661 atcataaatt taatattggt attgccgcac aacgaatttg gaaaggctta ggctttggtg
    21721 tcaattttaa atgggtaagc aagtataatt gggaaagtac atttggtgat ggcgctgtac
    21781 caagctacca catgctcgac atgcaagtaa gttatgaatt tccaagatgg tttactttgc
    21841 aagttggtgg ttctaatatt tacaataata aacacattga agcttatggc tcgccaatga
    21901 ttggtgcgct ggtttacggc tcaatgttgt ttgatttaga acgaaaaggt aatgtagaag
    21961 aacgctggat aaagaaaaag aaataacaag ataacagaaa tagcagtatg ttgtagctta
    22021 tattaagttg tacgcaagaa ttacatttgt atgataaaat tatttatttt tacgtctgat
    22081 gagcaaaaaa ttaactatag ttgttcctgc ttacaacgaa gaaaatacga ttcagaatat
    22141 cttgaataga attaatgagg tgcaactcat tcaacaaatc caaaaagaaa tcatagttgt
    22201 aaatgattgt tcgaaagata aaacagaaca aaaagtacaa gcgtttcaac aacaacatcc
    22261 tgagttgaat attgtgtata aaaaacatga agtcaataaa ggcaaaggcg ccgcactacg
    22321 cactggcttt cagagtgcca ctggcgattt tgtcattgtc caagatgcag acttagaata
    22381 cgatcctaat gagttcaata ttttgatgaa gcctatttta gatggctttg ccgatgtggt
    22441 gtatggctct cgatttattg gcggaaagcc gcatcgtatc ttgtttttct ggcataccat
    22501 tggcaataaa atgttgacat tcgcttctaa cgtgtttacc aatctgaatt tgacagacat
    22561 ggaaacgtgc tataaaatgt tcaaacgcga gattatacaa agtattgatt tgaaagaaaa
    22621 tcgttttgga tttgagccag aagtgaccgc aaaagtggct aaaattgcta aagttaggat
    22681 ttatgaagtc ggtatttcct actatggtag aacctatgaa gaaggcaaaa aaataggcat
    22741 gaaagacgct ttccgtgcat tgtattgcat tcttagatac aatttatttt cctaatactt
    22801 cgtcaagttg gcgctcgtga tgtgcataat gctcactcac gaaatgtaat acttgttgta
    22861 tgttaaacca gccagaacgt ggatgtttaa agatatactt atcttctaat tcgagaggca
    22921 agttgattag caattgcttg aaatcatttg tgttttttgc aaaataatct tggatttctt
    22981 taatgcttac atgttcagga acactgctga ctactttcgg tgctttaaat tttataggta
    23041 atttcaaaag tacaactaac aaaatatttc gaaacacact gccaatacta ctttgtagtt
    23101 gcactttatt ttctgccaaa tttttctgca tcacgatgat agtaccactt tctgcaaatg
    23161 aacaatgata aagcgtttgt ccagcactcc agcctccatt agttgcaact ttgttcaact
    23221 gttctgcatt caaatttgca actttttgca agaacttgtt ttttatcgtt tctatttctt
    23281 gatatttttc tgctaatgcg atactcatgt aataaattta cacaaatcta actacttatt
    23341 acgttctact tattactttt ataacatgtt tctcgatttc tttttgttgc tcaaagaaga
    23401 aggcttaccg gtaagcatcg gcgagtattt gaatttatta gaagcattga acaaaaaagt
    23461 tacgcagttt aatgtcgaag aattctattt tttatccaaa actattttgg tcaaacacga
    23521 acaattttta gatagatacg atttgttgtt tggtaattat tttaatggaa ttgaaattag
    23581 cgaagcagaa aaaaaagaaa ttccagctga ttggttgcaa aaagaaatag accgtttgtt
    23641 tagcgaagaa gaaaaagcca tgatagaaaa aatgggcggc ttagaaaaat taatggaacg
    23701 cttacaacag cttttagaag agcaaaaaga aaaacaccaa ggtggcaata aatggatagg
    23761 cactggcggc acgtcgccat tcggcaataa tggattcaat ccggaaggag tacgcattgg
    23821 cggcggcggc ggtggcagaa ctgctgtgaa aatctgggaa aaacgtcagt ttcaaaatta
    23881 tgctgctgat gtagaattga atacacgtaa tataaaactg gcgctgaaac gcttgagaat
    23941 actgacacga gaaggcattg aagacgaatt ggatttacat actaccatag aaaaaaccag
    24001 ccgaaatggt ggctatctcg acattgaaat gcaacctgca aaaaagaacc gcgtgaaagt
    24061 tttattgttt tttgatgtcg gtggttctat ggatccttac attgaattgt gtgagcaatt
    24121 attttctgcc gcaaaatccg aattaaaaca tttagagttt ttttattttc ataattgtgt
    24181 gtatgaaagt atatggaaag acaataactt gcgttacaca gataggattc ctacgttcga
    24241 tatcctacat aaatttaatc aggattatcg tgttattttt gttggcgatg ccacaatgtc
    24301 gccctatgaa ttaacagcac taggtggcag cgtggagcat tacaatgatg aaacaggcat
    24361 agcatggttg caacgatttg tgactaaatt tcctaacatc gtttggctca atccaagcaa
    24421 tgaatattat tgggacggcg tgcctacgat taaagcaata aaagaaattt ttaaagatag
    24481 aatgtttccc atgacaattg acggattaag caaagccatg aaaacattaa aaggaaagaa
    24541 aattattcat taaaatctaa ttactaattg tgcggtttat ataaaagaaa aaatgaataa
    24601 aaaagtataa ctagttgaaa tttgtaggat gtaagaagcg tgggacggca aaaaatcgcc
    24661 agcaaagcgt ggctaaagcg tggagcgata tttttgcctt gtcttttttg gttacttttt
    24721 ttgactaaga aaaaaagtga caaaagtttg aacttgataa atagttgaat ataaaaaatg
    24781 caaactaaat ttaaaacgca caacgcgtta ctaattacta attactaact actcaatact
    24841 aaaatatgaa cgaatttaaa ggaaccgaaa attatattgc aacagacgaa ctacaagttg
    24901 ctgtgaatgc tgccattgca ttggaaaaac cattgctgat taaaggcgaa cccggaacgg
    24961 gaaaaacgtt gttggcattt gaagtagcta aagcattgaa taaacaaatt ttcacatggc
    25021 atattaaatc tactacacag gcacagcaag gtttatatga atatgatgca gtagcacgtt
    25081 tgcgcgattc gcaatttggc gatgagaaag taaatgacat tgcgcattac attattaaag
    25141 gaaaaatgtg gcaggctttt gaaagtgaag agcaatcagt tttgttaata gatgagattg
    25201 ataaagcaga tattgaattt ccaaacgact tattactgga attagataag atggagtttt
    25261 attgttatga attgcaaaaa acaatcaaag caaaaacacg acctattgtt atcataacgt
    25321 ctaataatga aaaagaatta ccggatgctt ttttacgcag atgttttttc cattatattc
    25381 gttttccgga gcgacaaaca atgattgata ttatcaatgt gcattttcca aaaatagaag
    25441 acgagttgat ggatgaagca ttaaaagttt tttatggctt gcgcgatatt catggtatta
    25501 aaaagaaacc gtctacatca gaattgatag attggattcg attattaaag cttggaaaaa
    25561 acacgaaaga agatttacaa aaaatagatt atgtaaatgc acaaccacct tacttcggtt
    25621 cactactgaa aaatgaacag gattacgaac acatcagcaa agtaaaacgt gccggacaaa
    25681 atacctttaa gaggttttag cagaatagtt tattctattt tatatagaat cgttacgcga
    25741 tcttttccgc tgtttatatc ccagtgccaa aatttcttta tttttttaaa agaaggattc
    25801 gtttcaatgt cttccagttt aacatcacat tctacaggcg cataccaatc gtcccataat
    25861 acatagcaag gcttcttcat gacgttatta tgaaaatcat ttctgaaatc aggatgtatt
    25921 ttctgatcaa aatagtctct gttcaacaca atactgatat aaggtgctgc ataatacata
    25981 ggcaaattgt aacagtctct atgatattgc cataaataat ttccgatttc attctggcat
    26041 ttttgatcac ctcgtgcttg taagtctctg tcaggaataa cagcatactt agtatatcca
    26101 aaattagtag ctacgactaa aattattaaa acaccaatag tgtgagacgg atatttcgtt
    26161 tcaggaataa ataggaaaat gtcatttatt actttggcac agataataga aattaaaggt
    26221 aaaacaccaa ccattactct aattaaacca aacgaatgaa aaataccttt gtaccaggca
    26281 aatgaatgaa agaaaaaaac gctgaaaaag attccgtaaa tcaaaaagaa aaattctagt
    26341 gtaaatgcaa cctctttttt aaggataaat ttaagtatac tgtaaattag acctaatagc
    26401 attcctaacc ataaaatgta atataaaatg ataccggtag aaaaacggat gttgttaata
    26461 aaagcatccc aaggcccgct gccatagcta gtagtaccta ccgcataagg attctcattg
    26521 aaaatccata acaacgattt atcgtaaata atgaaaccca atagtgaata tacgacatga
    26581 ccaaccataa gcaaaggcaa tattttccat tcttttttca agagcaaata aatgaaaaaa
    26641 accaccataa caagtaatcc ttctgaacgt ataaatggta aaaaggatag taacaataaa
    26701 gcaattatct ttttatcgta taaccacaaa tgtatcaaaa gaattaatgc aaaagcaaat
    26761 agaggttcgg ttaatccaga taaagaatag ataatttgat gcggaaaaac tgccaggaat
    26821 aatggcacaa gccatgcatg ttttatacct aatttttgtg caacacaaaa agaaagataa
    26881 atagataatg cattgatgaa aatattaaaa acctgtatgc cgataaatcc aaattgagca
    26941 ggaatacaag taataagtac ataaacaggt tttgcccaat gatgaaaaaa atgatgtgta
    27001 tgctcaaatg catactttga aaacagaaag tgcataatac tgtctccttc atcgccggtg
    27061 ccggaatacg tataggcaat tacaatcatc accacaaaaa ataaaacagc aattgtaatt
    27121 gctattcttt tttgtgagga aacgttattc ataaacaaat attctgaatt ttatattctc
    27181 tatcatgtcc tgccattgtt ggtatttagt ttacggagcc aaagcgaagt aaacgtcacc
    27241 aacaattcta tgaattagat tctctcatca tgcaataccg cgcgtttcac caaaggattc
    27301 tcacgcattt ctgcaaagtt ggctctttgg ttgaaaatat ctattgcagt gtaaattgct
    27361 tttctgaatg aagtttcatc ggcaatgcct ttgcctgcga tgtcttcagc agtgccatgg
    27421 tcaggtgaag tgcgcaccac atctaagcct gccgtatagt tggtaccatc ttctgccgca
    27481 atatatttaa aaggaatcaa accttggtca tgatacatgg ctaaaatcgc atcaaacttc
    27541 ttgtaattgc aagatgcaaa aaagccatcg gcaggatatg gaccaaatgc cataatgcct
    27601 ttgtttttgg cctcttgcac agctggtaaa ataattgttt gctcttctgt tcctattacg
    27661 ccaccatcac cagcatgcgg atttaatcct aacagagcaa tacgtggttt gtcgatacca
    27721 aaatcttgca ttagtgaagt gtgcatcaaa tgtaatttat gtacaatttt ttctttgcta
    27781 atttttgaag atacatcttt aatggcattg tgattagtaa ctaaaccaac acgcaaatca
    27841 tccgccacca taaacattaa attgtctttt gcaccaaaat aattcatcaa gtattcagta
    27901 tgacctgcat acggaaattt gtcagaatgt atgacattct tattaatcgg cgctgtaacc
    27961 aaaacatcaa tctgcttgtt ttttaatgca tcgcatgctg cttctaatga ttttagcgca
    28021 taattgcctg taatgtctgt tggcgtgcca ggtgtaatct gaaattcttc ttcccaaact
    28081 ggaataatat tcagtgaatt atgacttaga ttgccatatc ctttgataga tgaatattga
    28141 aatttatcga tattcaacat ttttttataa taagatagaa cacgtggatt gccatataaa
    28201 attggcgtga agtgtttata tattagttca tcagaaaatg ttttaatgat aatttcagga
    28261 ccaatgcctg ctacatcacc cagcgttatt cctacttttc ttttctggtt ttggttgctc
    28321 atcgttacac agattttgca caaatttaaa caataatctg acaccatacg ctgttgccca
    28381 ttttccacga taattatagg gtgctgatac atacgctgga ccagcgatat caaagtgcat
    28441 ccaaggataa tcggtaaaat gttttaagaa gacacctgca gtaattgcgc cagcgtccat
    28501 aggtcctaaa ttttttaaat cagcaatatc gctcttcata taatctgtgt attcgtccca
    28561 cagcggaaat tcaacggtgc gctcaccggt gcagttagca gcctttttta atttcttttt
    28621 aattgacttt tctgctgtgc ccataattac gcttgcttcc ttcccaatgg cacgcatagc
    28681 ggcacctgta agcgtagcaa aatccaatac taattctggt ttaaagcgtt tggcataatg
    28741 caatgcatct gccaatatca agcgaccttc cgcatctgta tctaatactt ctaccgtggt
    28801 gccgtcatac attgtaataa catcactcgg acaaattgct tcatagccag gtcggttctc
    28861 tactgccgga attaagccga tgacataata cggtaattta gcagtggcaa tagcatgcat
    28921 tgcacccact acagcagccg agcccgccat gtccgatttc atcatgtcca tgctgttgcg
    28981 tgtaggcttt aatgataaac cacctgtgtc aaaaaccaca cctttgccca ctaaaactaa
    29041 tgcttgttca tttttatgct ttgcaggttt atattctaat atattgaaag tagcaggcgc
    29101 cgaactacca gcatttactg ctaatacgcc acccattcgc tcagcttcga tttctttttg
    29161 atctaagacc gtaacactaa atccagcagc ttcgccaagc ttttgaattt cttttgaata
    29221 ttgcgtagca gtcaaaaatg aaacaggctc attaactaaa tcttttgtaa tgcgtacact
    29281 ttctgttagc aagttgattt cacttacaat agtttccgat atcaatattg gatcgatgtg
    29341 tacagtaagc gtaaagcctt ttggtttagt tcgatatttt aaaaactcgt aatttgacaa
    29401 gttaatacct aataaaatcg cagctaagga atcttggtga atatcgccta aaacttctaa
    29461 gctaatgtct tgttctttgt tttctttgca tactttgaaa atgctattgc caagaatacg
    29521 caatctttcg tgttgcttgt cttcctctat ttttgcatcg atttctatcg atgataatat
    29581 gtaggtcttt tgctgtttgt tgaaactgaa aagcgtaaca ccatcgttga ttttttgttg
    29641 aattgccttt ttttcttcaa tatcgaaaaa tggtaatata tttaagttta ctttagtaaa
    29701 caataccaaa atggcacgtt tggctggttt tgctgaaatc tgtaacataa tatctatctt
    29761 tagggcgcta tgtctctagt tggagagctt agtgtacaaa tttaaacgtt atacccaaaa
    29821 aatctatatg caaaagcagc gctttgtgac agccttaaaa tcgtatggac agcactttct
    29881 gacagataaa ttggtgttgg aaaatatcat tgctgttatt aaaaaatacc atatagatga
    29941 agatgtaata gaaatagggc ccggcactgg cgcacttacg acccatctgg cacctgaatt
    30001 tagcagttta cgttgcatcg aggtggataa gcgttgtatt gaatatttac aaacaacatt
    30061 gcttaaaacg catcagcacg tacaaattat agatgctgat tttttaaagt tggatttgcc
    30121 aacaatacta caaaaaccaa cagctgtagt gggaaatttt ccctataata tatcttctca
    30181 aatcgtgttt agcgtgttgg ataattataa gcaaattcct tttatggtcg gcatgttcca
    30241 aaaagaaatg gcggagcgca ttgcttcgcc acatggcagt aaagaatatg gcgttataag
    30301 tgtgttcgca caattgttgt acgatgtgaa gttggagttt gatattgcac cacaaagttt
    30361 tgcaccacca ccaaaagtaa tgagtagcgt tttgtcgatg aagcgtaaag aaaatataca
    30421 attagatttt gatttcaagt tgtttaaaaa tattgtaaaa tcaggattta accaacgtag
    30481 gaaaatgctg cgcaatggat tgagtggcat cgtagcaaag gatatgttgc aacatgaatt
    30541 ctttcagcaa cgagcagaac agctcagtgt gcaagatttt atcgcactta caaaaatgat
    30601 tgaacaatca aaatgagaaa agtattaata acagctgcca ttgattcgta tattcaactt
    30661 cgatttgaag ctttaggttt ttgtgtagat gtttatgaag aaatacagcg caacgaattg
    30721 ctggaaatta tagcagcgta tgaggtttta atcataacaa cctatacaaa agtagataag
    30781 gaattattag atattgcaac taatcttaaa atcgtcggac gtgttggaag tggtatggaa
    30841 aatgtagatg taatttatgc acgacaaaaa aatataagtt gcgtaaattc gccagaaggc
    30901 aatgccaatg cagtaggaga gcatagttta gcgatgttgc ttgctttgtg taacaagtta
    30961 tataaagcaa ataatgaatt aaaaaagggc atttttttac gcgaagaaaa tcgtggattt
    31021 gagttagacg gaaaaacaat tggcattatt gggtttggtc atactggtca agcttttgct
    31081 aaaaaacttc gtggctttga tgtgcaaatt ttggtgtatg ataaatacca aaagacagca
    31141 acaaacacgg cacagcatgt gtcattgcaa gaaatccaaa agcaatgtga tgtgattagt
    31201 tttcatgtgc catacaatgc tgaaacacat cattactgta atgctgaatt tttatcaaag
    31261 tgtgtcagaa agcctgttat tattaatact tcacgtggtg ctgtagtgca tacattagac
    31321 ataaaaatgg cattagaaaa caaaaaaatt aatggctttt gtattgatgt tttcgaagat
    31381 gagccaatca gcaaaaatgt aatcaatagc aaagaaactt accaagattt attagcattc
    31441 gataatgtag tggctacgcc acatattgct ggctggacaa tagaatcaaa atataaactt
    31501 gccaaaatat tgatggataa aattgaagaa tgcttataaa atatttatag cctaatatga
    31561 ttctttctta cttgtttttc ttttttcgag ccaccatgca atgcctgcac ttgccaataa
    31621 aatatagaat ggaatacttg gcagtctata acgtgcataa gaaatgatac cactgattaa
    31681 tgtaaaatat cctgcagagc ctaaaataaa taacatcaat aaatattgtt tttctttgag
    31741 catacgccaa ataccaattg ctgcaaaaac ataaacaatc aataaaaaca gggcaacaaa
    31801 taagcctaaa ataatttcgt atatcgtttt ttcgagaaag aattttttaa ttaaagcaaa
    31861 aacaccattg gtatatttct cttcctcgct aaatgtttta gctggaatat gcagcacttc
    31921 tgtcaatatg tgagtgcctg tgctgagatg tatttttacg gttccgatta gatgtgtttt
    31981 caaataatcg ggaaagtttg ctttcaaaat tttactggct aattcaccat catatttttc
    32041 tttttcaaat ggattagcat caggtcgcca gcccattttt aaaagctccg ttttaaaatg
    32101 agcatttaca gtatcgatag gcagttttaa tctttttgat tcgtaataac ttgcattcca
    32161 aaataacaga ttgtaccctt tgatattgga caatgcaaaa tgattgtatt ctttataatt
    32221 acgcatacac catggcgcaa tcataataaa ataggcaatg ccgataataa ttgctgcttt
    32281 ccaaccattt ttccaagctt tttgaaaata ccacatcaag gtgaaaagta gaacgccagc
    32341 aatataatat tgcgaaattg gtcgtattaa tgtgctaata ccatatatca gaccagtaaa
    32401 aataaaatag ttaagtttat tggttttaat ggctttgata taataccaca atccagttag
    32461 aaatactgta gtataaatag tttcagttaa aatttgatag atgaaaatgg tgtgatgtaa
    32521 atcgagtgaa aatagcaaag ccgcaataaa tcctgcggtt tctgagaata tcagctttcc
    32581 aattttatac attaaaacaa tagacaataa attgagaaaa atttgtacta tcaatgcata
    32641 ataaggttgt tcgccaaata ttgcatatac gattgccaca aaaaacggat atgctggcgt
    32701 acgaaaggca tcgccacaaa atgaatattc attttttatg cataaagcta gattgtgata
    32761 tcctagagag tcgaaaatta atagctggtt tttgataaca tctgcttgcc atggctcaaa
    32821 tcgtaaaaaa atcaacactc taacgatgaa tgcgacaagg agtaggtacc ataacttttt
    32881 tggcagtgat aaaaatgtga catcaggcgt tttcatttgg taaatgtact tgaattgtta
    32941 aaactatcaa aagctaagtg catattagtt gcttattaag gaaatattat ctaaatttgc
    33001 gacaaggcat gcttcggaag aggcgtgtct atttttttca ctaaaaaatc aacattatgt
    33061 cagaagtagt atatgtatcg gaagaaggct tggcaaaaat gaaagaagaa ttggcgcaat
    33121 taatagcagt agaacgtcca aatgcatcga aagcaattgc agaagcacga gaaaaaggcg
    33181 atttgtcaga aaatgcagaa tatgatgcag caaaagaagc acaaggttta ttggaattac
    33241 gcattactaa attacaaggt gatataggca acgcacgcgt tctagatgct tcaaaaattg
    33301 atacttcaaa agtgtctgta ctttcaaaag tgaaggtgtt aaatactaaa ttgaaaaaag
    33361 aatttactta tcatttagtg tctgaaaaag aagcggattt aaagcaatta aaaatatcag
    33421 taaaatcgcc aattggtgca gcattgttag gtaaaactaa aggcgagaaa attactattc
    33481 aaattccggc tggcgcaatg gaactccaag ttttagatat cagtatttaa tccaaaaatt
    33541 taatcaataa ttatggcatc tatcttcaca aaaataatta aaggcgagat tccttgttat
    33601 aaaatcgctg aagatgaaca tcattttgca tttttggata ttcgaccatt gcatattggt
    33661 cacactttgg tgattcctaa aaaggaaata gattatattt ttgatttaga agatgctgaa
    33721 ttacaaaact taattgtctt tgcaaaaaaa attgcagtag ccttaaagaa atcgattgca
    33781 tgtgagcgca tcggtattgc ggtaattgga ctcgaagtgc cacatgcgca catacacttg
    33841 tcgccaatta attctataca cgatttagat ttcaaaaatg caaaagaaat atctaaagat
    33901 acaatgctag aaattgcaga aaaaataaag gcaaacttag acctctaata ggtattctat
    33961 tttgttgtgt ctatattctc gaattttggt atttccttta tttcaaaaaa tttcaacgct
    34021 tttaattttt caatttctga tttattgata cgtacatagt aatgtcttcc atttgtgcaa
    34081 tgacatgcat cacacgaaat gatatcttta ttaaagcttg attgaatttc taaaacagca
    34141 atttcttgac ctcttaaatg acccttaact gcacttctga gttgctcctc tgtaggcttt
    34201 tggaaccaat taaaatgcca aggattttca catttagttt catcgtattg catccaaact
    34261 tcttgaggat tgattttgct aacagctgtt gctgtatttg tttttttctt acaacatttc
    34321 tttttcttgt ggcattttcc aaaagaaagt aagcataaaa ataataggct gagtaataat
    34381 atcttcttca ttaatttttt atagcaaatg aaataccaat tttttatagt aattataatt
    34441 gaataaaact acatttatca gccactgata tagtagcttg tttaccaata aatacagcac
    34501 gattgtcggc aatatgacca cacggaaaat taaaacaaac aggatattca aattcggcaa
    34561 catgttcgta aataatttct tctgcacact taccataagg tacagtatta tctttcatgt
    34621 cactcaatcc gccgactact aaaccagcca aatgccttag ttttcctgca cgtttgagtg
    34681 ctaccatcat tctgtcaata tgatataaat attcatctaa atcttctaaa aacaatattt
    34741 ttttgttggt atctaatatt gtttttgttc ctaaattggc atataaaata gatagatttc
    34801 caccaactat ttcaccttgc attatgccat ttctgttcaa gctatgtttg tcaaaagtgt
    34861 attgtaagct attgccaaat aaggcatctt ttaatgattg aatcgcagca gctgtgttat
    34921 tcggaaagct gattggcatc gtggcatgca aactttccat accgatattg cacgataata
    34981 aggtgtgtaa aaatgtgata tccgaaaaac caactatcca tttcggcgaa tccatcaagg
    35041 ctctgtaatc taaatcatcc atgattcgca ccgtaccata gccaccacga gcagcaataa
    35101 ttgcttttat agaagtgtca ttaatcatac gctgcaagtc gtcgcgacgc tctgcatctg
    35161 tgccagcata ttgattgtct tcttttccta cggtattgcc aatatgcaca tttaaatccc
    35221 aacttttaaa aatgtcgata gcaaattgta tttctgccaa tgtgattttt cgagctggac
    35281 aagtaatgcc tatggtgtcg cctgctttta aaaatgatgg tctaatcatg agttataagg
    35341 tgcaagctca aaagtacaat attttgattt ttatatggtg taaaagatac taaattttaa
    35401 gctgcttaca caaatgatat aatcatatgt attgttgaat gaaaagcgct ataaaaatat
    35461 tgctatcttt gtggcatgtc aactcctaaa agatacacag ttacagctgc tttgccttat
    35521 gccaatggcc ctttacatat aggacattta gcaggagcct acattgcagc ggatatttat
    35581 acacgttatt taagacagca aggcaatgat gtgatttttg tatgtggtag tgatgaacat
    35641 ggcgcagcga ttaccataaa agcgaaaaag gaaaatacca caccaaaagc cattgtcgat
    35701 aaatatcacg ccatcatcag gaaaagcttt gaagatttcg gtattgcatt cgatatttac
    35761 catcgcactt cagaacaaat tcattatgac acatcacaag atttttttag aatcttaaat
    35821 gataaaaatg ctttcgaaat tatcgaaagc gagcagtatt acgatgctga agctaagcaa
    35881 tttctggctg atagatatat tttgggcact tgccctacat gcgcaaacga gaatgcttat
    35941 ggcgaccaat gtgaaaaatg tggctcatcg ctcagtccaa ctgatttaat caatccacgt
    36001 tctaccttga ctaattctac gccaatttta aaaactacta aacattggtt tttgaaatta
    36061 aatgagcagc aacacgacat agaaacatgg ctggaaacga aaaaagatat ttggaaaaat
    36121 catgtgtatg gccaatgtaa atcatggctg aacgaaggac ttaagcctcg tgctatgaca
    36181 cgtgatttag attggggaat tccggtgcca caagaaattg aaggcagcga aggcaaagtg
    36241 ttatatgtct ggctggatgc acctatcggc tatatttctg caacaaaaca attgttttta
    36301 gagaaggcac aacaagaaaa tattttctcg tataatcaaa gagaatttgt tgatgtaagt
    36361 gcagatgatt ggaaaaaata ttggcaatcg gaagacacga cattagttca ttttattggc
    36421 aaagacaata tcgtttttca ttgtattact tttcctgca
//
LOCUS       DAOQQV010000014        36362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_324_length_36362_cov_8.541879, whole genome shotgun sequence.
ACCESSION   DAOQQV010000014 DAOQQV010000000
VERSION     DAOQQV010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 36362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36362
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_324_length_36362_cov_8.541879"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1926)
                     /locus_tag="PLL59_02925"
     CDS             complement(223..1926)
                     /locus_tag="PLL59_02925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HMY22787.1"
                     /translation="MKTVTRKKRNYLPENLVVHGWQNIESFYQELQQRPIESKEAVLQ
                     WLADRSETDAVVDEEYRWRYIRQTCDTDNETFKDAYEDFIQTIMPNWMSIGNELNKKL
                     ATSAFVKDLDQERFFVYLRHLQAQLQLFREENIPLSQQVQLLSQEYGATIGAMTIEHE
                     GKEFTLPQAGVFLQQQDRNLRKSIFEKVNNRRIQDKEKLNNLFDELLKIRHQMALNAG
                     FTNYRDFMFAELGRFDYDVHACEQFHKAIKSEVIPLVNEIYAKRKQALQVEQLFPYDL
                     EVDVANLPALQPFSTQDELIEKSVKCLTQVDDYFAECIAIMDNMHYLDLNSRKGKAPG
                     GYNMSLPEIGVPFIFMNAAGTHRDLETMVHEAGHAVHSFLMRTLPYNFDQEISSEMAE
                     LASMSMELMTFDGLSAFYNDADKKRAIETHIEGIITMLPWIALIDKFQHWIYTNPNHT
                     STERENKWLELHKELSADIVDWNLYEDFRKILWHKQLHIFEVPFYYIEYGIAQLGAIA
                     VWRNYKQNKAVAIDNYKNALQLGYTKSIPEMYKTAGIQFNFSREYVKELMDFMKNELS
                     N"
     gene            complement(1996..2508)
                     /locus_tag="PLL59_02930"
     CDS             complement(1996..2508)
                     /locus_tag="PLL59_02930"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HMY22788.1"
                     /translation="MEFREIIAVTGVAGLKKLVANRNDGLILSELDDSNKKFYSNRQH
                     MFSPLENISIYTEDDTVLLLDVFIEMKNQKDSTPLVDANASNDDLRAYLGKILPTFDR
                     DRVNISDIKKLVKWYNALEGFDIFKKSAEGEENKSSVKTTTQKHHAPAKDMNVQQQSV
                     AKPRMTKNKV"
     gene            complement(2580..3869)
                     /locus_tag="PLL59_02935"
     CDS             complement(2580..3869)
                     /locus_tag="PLL59_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY22789.1"
                     /translation="MKNPFLLLVLFFTSITVNMYADDCNNGRYIQQVFNRVDITKDVK
                     YARKKQSDGQYIDLKYDVYQPHGDTLAQRPIMLLIHGGAYLKLLDHNSPDIVLMCDYF
                     AKRGYVTVSIDYRQEPNLLGLLSEEVMVKAVSRALIDTKEAVDHLMLTYQNGNPYRID
                     TSRAFIGGVSAGAVSSMFISYLDSLQQLPAQYQQWIVEANGDSADYILRHKFDLVKPK
                     ACISVSGAILDTNWINKNGIDMLIIHGSADEIVPYNYGNPFHIPTLPVLFGGKAQYPV
                     MLRKGIRVEFEDWIGRGHVPFMNLTFPDIIFNFINQPILDSTERHIAHFIYPWLNCNR
                     TTHIKQNLMQEKLAIFPNPSNGQFSIPIPKSASYQNWKVEIYDLIGQQIFQQAVAYPT
                     DFITINTTLAAGNYFVKMFYEQNNENIVYSGKITITQ"
     gene            complement(3885..5204)
                     /locus_tag="PLL59_02940"
     CDS             complement(3885..5204)
                     /locus_tag="PLL59_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY22790.1"
                     /translation="MYRIFYTLLLFLAFQSSIFATDCFNGRYKQKIFQDVTITSDVKY
                     AKLQRSDGAWLDYKYDVYQPKNDTAQNRPLVVLAHGGAYLEIPLLDKKSPDIVELALD
                     LTQKGYVVISIGYRLETNLLSLLSEENMIKAVGRAVMDARLAFCRIVDTTLHYNNPYR
                     IDLQSVFIGGVSAGTVSFLHGMFLDSISQMPNQYRQWIEQVVPNAQQLLDDKFCGANI
                     LGMINVSGAVLDTAWIKPNRQYPALLSQHGTVDVVIPYSYGHPFGLPMLPNLMGSSLI
                     DKRYKHLGLRSELETWVNYNHVPFVGGLNLGALFGQNPLAVVFNPYVLDSTKRHITNF
                     CYSLLTCDALTTGIKQHLMDSPISIFPNPSNGNFQIKIPFEAKYHNGTIDMYDISGKN
                     VYQTNFPVFTENVFIDKHLPNGAYFIKLAYQKDEEEYIYTSKINIVH"
     gene            5356..7383
                     /gene="uvrB"
                     /locus_tag="PLL59_02945"
     CDS             5356..7383
                     /gene="uvrB"
                     /locus_tag="PLL59_02945"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HMY22791.1"
                     /translation="MPFKIHSSYQPSGDQPSAIEQLSEGVLKGEKYQTLLGVTGSGKT
                     FTIANVIEKTQRPTLILSHNKTLVAQLYTEFKEFFPDNKVEFFTSYYDYYQPEAYVAV
                     TDTYIEKDLSINAEIEKLRLSTTTSLMSGRRDVIVVASVSCIYGLGNPAEYKNQIIRI
                     SKGQTLSRNTFLFDLVSILYARTTADFQRGNFRVLGDNVEVFLAYADYAYRITFWGDD
                     IEELESFDPVSGKRIEKMQDIAIFPANIYIAPRDQLPTIIHEIQDELTAHVEYFNKTG
                     KQLEGKRLEERVNFDLEMIRELGYCSGIENYSRFFDRRNPGDRPFCLLDYFPDDFLLV
                     VDESHVTIPQIGGMYGGDRSRKQNLVEYGFRLPSAMDNRPLNFGEFESLLNQIVFVSA
                     TPADYELMKTQGEFVEQVVRPTGLLDPPIDVRPSLNQIDDLLDEIQKRISINERILVT
                     TLTKRMAEELAKYLSKLNIKCKYLHSDVDTMERVEIIQGLRIGDFDVLIGVNLLREGL
                     DIPEVSLVAILDADKEGFLRNERSLTQTAGRAARNANGLVIFYADVMTKSMQKTIDET
                     ERRREKQIAYNIQHHITPKTVLKSKEEILQRASILDIRQTAETKTEKKHREHEESMII
                     AAEEQGEYLSTAQIEKKIAQLKKAMQRASQQMDFMEAARLRDEMFKWQKQL"
     gene            7365..8255
                     /locus_tag="PLL59_02950"
     CDS             7365..8255
                     /locus_tag="PLL59_02950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY22792.1"
                     /translation="MAETIIKNKISMNRKTILITGANSGLGFEAAKQLAKQGHEIILL
                     CRNKEKGEIAVSEIKSYSNNQNIHLYTANLASQKSIETVAQQIKKDISKLDVLLNNAG
                     GVFNNFQLSEDGIEMTIANNHFNYFWLTYYLFDLLKNGKDARIINVASDSHYSAKKID
                     IESFYQKKGNYFVLSAYEQSKLANVLFTYTLAEKANPYNITVNALHPGFVYTPIGEKN
                     GNKFFGMVWSAASKLFGLSVEKGARSHIYLASSDEVKDISGKYFHNCKAKKSSSISYD
                     KNLQAMLWKESERKSGFTFL"
     assembly_gap    8416..8515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8516..8762)
                     /locus_tag="PLL59_02955"
     CDS             complement(<8516..8762)
                     /locus_tag="PLL59_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22793.1"
                     /translation="MAKKTASAKPAAKKAPAKKAAAPKKAAAPKKAAAKKAPAKKAAA
                     PKKAAAPKKAAAPKKAAAPKKAAAPKKAAAPKKAAAPK"
     gene            9411..10391
                     /locus_tag="PLL59_02960"
     CDS             9411..10391
                     /locus_tag="PLL59_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22794.1"
                     /translation="MKKLIVFIFVGVIAFISACSSCNNEKEKTKPVEIDNIKESYKML
                     PSEQQSVIDVVYRWNAAHDSTQIDSLEQFYNDPVFFFKKRIARASVLASKKYKISHAD
                     TYRERIIGRIGIYASDTGDYKCEFLKLVTINSKPNVYHSYLIFKKQADSTWRIIVESD
                     KEADLRPELVSLFEKFEESESTSSGDFNGDGEAEDLIVLKPTQDSSGNYTSKQTKISF
                     TNLDLPEITIQNCVGVNVLNENDVDGDGADDFSVVIKKEDGDVGDVILYSFKRGNWQE
                     LAKFKAPQEEVFSSRQQLIEFAGNGNIKIRSAEKTIDNRDTLVLKTISTW"
     gene            10429..11031
                     /locus_tag="PLL59_02965"
     CDS             10429..11031
                     /locus_tag="PLL59_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HMY22795.1"
                     /translation="MYIPSYYKQKDIDKTIAFMQAYNFASLISIQDNIPIATHLPFII
                     EKESEALVLYSHLSKANPQWKTFSENNILVIFSEPHAYISPSLYSGKQEVPTWNYVAV
                     HAYGKISILPSDEEKLAVLHKQMQSYESEYIAQFKALDHQYIQNLLQGIVAFKLEITD
                     LQAKEKLSQNKNDIDRKNVKQHLLESDDTNKQALGNMMAD"
     gene            11096..12463
                     /gene="hemN"
                     /locus_tag="PLL59_02970"
     CDS             11096..12463
                     /gene="hemN"
                     /locus_tag="PLL59_02970"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HMY22796.1"
                     /translation="MLSYDLVDKYNVPVPRYTSYPTVPYWEEPMNDIDDWKNQIQKAI
                     ACNKTKEGISLYLHLPFCEQLCTYCGCNKRITKNHQVEDKYIAALLTEWEQYKAIFGD
                     ALCIREVHLGGGTPTFFSPKNLYLLLSALFKDVRIHPAHEFSFEGHPNNTTFEHLETL
                     YKLGFRRVSFGVQDTDAKVQIAINRVQPFSKTKQVTEWAREIGYESVNFDLIYGLPFQ
                     TEESIRKTIEAVITLLPNRIAFYSYAHVPWTKKSQRAYDENDLPKGKDKYTLYEVGKD
                     LFLQAGYADVGMDHFALAGDQLLISKQNGTLHRNFMGYTTTNTEVLIGLGVSAISDIF
                     FGFRQNLKTVEEYYEAIEKDTLPIIKGINVTDEDIVYRKHILNIACKGKTDWTNEIEL
                     TDTMIKHLDALQSDGLITWQNASLEVSDLGWSFLRNICSVFDKKMNSTKNDSPSDTPK
                     FSQAV"
     gene            12485..14080
                     /locus_tag="PLL59_02975"
     CDS             12485..14080
                     /locus_tag="PLL59_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HMY22797.1"
                     /translation="MSWQHTTSIYQIYPRSFYDSNGDGIGDLQGIIQKLDYIQQLGFE
                     TIWISPFYKSPQDDFGYDIADYYTISPEYGTLQDAEMLIHEVHKRDMKIVFDMVMNHT
                     SNQHHWFLESKKNKTNPKADWYIWRDQPNNWKSIVGPKAWHYCEERKQYYFASFLPFQ
                     PDLNYRCPEVKNAMFDVCRFWLSKGVDGFRLDIFNCILKDKDFKNNPFSLWHAIPSED
                     YPGGNFQIRKYSLNLPENFMLAKELRQVVDEFPDRLLLGEVFGKHELKKKYLGENNDG
                     LHLIFLFDIVMFTFTANFFRKKILEFEQQYPSPYMPTVVFSNHDQLRSIKRIGNKLEK
                     AKLLALLQFTMRAVPTVYNGEEIGMMDGNIAIKDGKDPVAKVYSWVPQFIADRLPVGI
                     NRDNCRTPMQWNHTEKNAGFSTADTTWLPVNTTIENRNVAQQQADKNSLLHTFKKLIS
                     LRKNWRALHRGNIEIIYTGNKNILAYTRTVEEEKLLVMMNFSNSKISNKINQPIKEIV
                     FSINNISTFDGVLLPYDGVILKI"
     gene            14111..14854
                     /locus_tag="PLL59_02980"
     CDS             14111..14854
                     /locus_tag="PLL59_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22798.1"
                     /translation="MSATQDVHIIEKFREMIGKRYAYHDLINRFEIPASITEEMIKEI
                     ENYFLTIIYPPAPERKELEEAFKDLAAYVKQPRKIWNLFGDMARALFKFGRHFMTALK
                     AGMDALDSFVGAKNFENSMALIANKNGIRPPMSDEDFEDTMYQLPREEVEKFIKDVKS
                     LFGAMVNTTLLSKTLDILDHVIHTMEKKPNVFPQKEVEGIKLGKSLLQKGYDLFSKYD
                     DTTKNLIVDIIYKNEMWYVDYVYKKKEGK"
     gene            14916..15296
                     /locus_tag="PLL59_02985"
     CDS             14916..15296
                     /locus_tag="PLL59_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMY22799.1"
                     /translation="MARKIIYTDDAPEPIGPYSQAVYINGLLFVSGQIPLDPFSGVLI
                     QDGIEEETEQVMQNLKAILHAAEMDFSNVVKCSIFLTDMRDFPKVNEVYGKYFKSQAP
                     ARETVEVSVLPKNAHVEISCIAAR"
     gene            15320..16072
                     /locus_tag="PLL59_02990"
     CDS             15320..16072
                     /locus_tag="PLL59_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22800.1"
                     /translation="MKQLILIIICIASIKQTLTASENIEICNAAKDSAAALFERGGLG
                     FYASSKMNPDLYNYIIITKENYKKTTVRISKSTSDTAKLMKYKLFSTSYDKNADKSQP
                     LDTSLDYKKCYNAAFQAKLDSVFKCDFFRKSDSILKAYDKAGKGYSAVEFKGGPSALQ
                     KYLDKTIVFPKDCKPNDSDKAYRVYYSFFVDENGSISDINLIKSNCKACEASILEAIK
                     KMPAFVPALEAGKPKKSKYILPYLKAYTKPKG"
     gene            16104..17168
                     /locus_tag="PLL59_02995"
     CDS             16104..17168
                     /locus_tag="PLL59_02995"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HMY22801.1"
                     /translation="MKIEEITFDSDDKNLFLIDDIQLKADAIRYSILPKLEVINNELI
                     SRITEMYDFDLFQNCAIGKTPQFRTSKSQRTKPVTLDYKHSIIWINGQRTKNKWKGLE
                     RINNKEAQITPTAIGIRLTTEGISSLFWFGYVKNFTEETYRKFYDFLDREIINVLSLL
                     NRSGCKFQRWYFEIIDIRKTLQQKFENGDYNILIGQPKAFEYPINYEKINEIILSNLM
                     LFPLFNAFLNISLGIEPNIKNDIEKAEKNFQSYYEKYFTEKQEETSVLSESDMQTFRE
                     KADNKIKVQAGIRWQVFKRDNWKCVACGRSAEENIILHIDHILPRSKGGKDEMNNYQT
                     LCETCNIGKSNKDETDLRKK"
     gene            complement(17334..18257)
                     /locus_tag="PLL59_03000"
     CDS             complement(17334..18257)
                     /locus_tag="PLL59_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMY22802.1"
                     /translation="MEVSIWYWIGFNLFVCIMLALDLGVFHKKSHAVKIKEALAWSAV
                     WVCFAMLFNIGVYYSFGKEKAIEFFTGYVIEKALSVDNIFVFVMVFSYFNIPKKYQHK
                     ILFWGIIGALILRAIFIFAGVALISKFHWIIYVFGAFLVITGIKMMVQDEKPLEPDKN
                     PLVRLFKKFMPVTNTLHGAKFFVSIDAVKHATPLFIVLLLIEFTDLIFATDSIPAILA
                     ITNDTFIVYTSNVFAILGLRSLYFALDGITAYFRFLKYGLSLILVFVGTKMCIADFYK
                     IEIVHSLAIILVIITISILASLLIKSKEINE"
     gene            complement(18369..18707)
                     /locus_tag="PLL59_03005"
     CDS             complement(18369..18707)
                     /locus_tag="PLL59_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22803.1"
                     /translation="METFSREVVLLNYTMQCVMSKVVAYLLLIFFFVCTNESNDTLLN
                     NNKTTIFHELQEESTKLQAVLETEQFQFVHQTSKQDYEEIGWFNYTQTAHHFPTGDYT
                     NTLDFPPELV"
     gene            18753..19775
                     /locus_tag="PLL59_03010"
     CDS             18753..19775
                     /locus_tag="PLL59_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMY22804.1"
                     /translation="MQATFNERFRQVVLFVILLALAILIFIQINFVVPGLLGALTLYL
                     LNRKLLFTLSFQKKWNIYIAASLLLLIDVIILAIPFGLAILFVVPKLQNFSSNAQELF
                     QGVKEIVVRIDEATGIELLSKENLSNIPAIVSSHLPDFLSTTASLFFNLSTMFFVLFF
                     MLINATKIESSLIKYIPLKPENKQKITAETKNIVTSYAIGIPILAIAQGFCAYIGYNI
                     FNISDPMLWGFVTCVCSVIPFVGSGLIWIPLVAYLFVGGHESDAIGLAIYCAIVVLNV
                     DNVLRLFLLKAFANIHPLITLFGVITGIKLFGFIGLIFGPLLVSYLLLMIKIYVSEFT
                     SSEVSE"
     gene            complement(19789..20463)
                     /locus_tag="PLL59_03015"
     CDS             complement(19789..20463)
                     /locus_tag="PLL59_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMY22805.1"
                     /translation="MSTKTYFIGGIASDERLFRYQMTAIENAVYLPFPKHDKHDTMET
                     YVKKFIPLIDTSQAFNIVGNSMGGIMTMELIKHIQAEKVVLISSVKCRKEMPFILKQL
                     KYTNLHKVLPGRGFIGSIQFGSLFKREVLKQPAMRKLAISMAKNNDTSFLYWCVNAIV
                     KWQGNEDYRKDILHIHGTKDEMFPYRNIQNAIPVQGGSHAMLLIQHKEVNELLKACLY
                     SENTSK"
     gene            complement(20781..21365)
                     /locus_tag="PLL59_03020"
     CDS             complement(20781..21365)
                     /locus_tag="PLL59_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HMY22806.1"
                     /translation="MKTGRLEAFSDGVIAIIITVMVLNMKAPIGATFSALKVVLPNFI
                     SYIISFIYVGIYWNNHHHLFQAIEHVNGKVLWLNLILLFCLSLLPFSTSWMGDNHFQS
                     IPVLLYGINLLLCAISFILLAKSAAKHEGENSVIGKALKSKNKENISLLLYLFAIAIA
                     TYFPIISLISYTIVAIIWLIPDTRIERQLHTPKK"
     gene            complement(21398..22276)
                     /locus_tag="PLL59_03025"
     CDS             complement(21398..22276)
                     /locus_tag="PLL59_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMY22807.1"
                     /translation="MATKPVEIVVNPREPHFVGDGFRVHNFIPSAYRLDMQRMDPIIM
                     LDYNSKFYFEPSDTPRGVGVHPHRGFETVTIAYKGKVEHHDSSGGGGIIGEGDVQWMT
                     AASGVLHKEFHEREWSKKGGDFQMVQLWVNLPAKDKMSTPKYQAIENATMSRYELEDK
                     GGEIEIIAGEYKGIKGSASTFTPLHLFNAKLHKNALATFDFPKHFNTALLVIEGEIIV
                     NESEPVKEDQFVLMANEGETFTIKATADAIVLVLSGEPIGEPIAAHGPFVMNTKQELI
                     QAFDDFNKGKFGYLEE"
     gene            complement(22344..22934)
                     /locus_tag="PLL59_03030"
     CDS             complement(22344..22934)
                     /locus_tag="PLL59_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY22808.1"
                     /translation="MNSFNFKTYLTSNIALDNDSIDNLMLHCKQQQIYKGDFLLRQGE
                     KCKHSFFVESGLLKQYSIDEKGKEHIIQFAPEKWFISDRESVYFDNPSNYFIQAIEDT
                     KVFVLDELFFQELSLSHPNFLDYNNRLLHNHIRHLQKRITQLLSATAEQRYLDFIQVY
                     PDISLRVPQTMIASYLGITPESLSRVRKELAQQFQK"
     gene            complement(22997..24016)
                     /gene="nadA"
                     /locus_tag="PLL59_03035"
     CDS             complement(22997..24016)
                     /gene="nadA"
                     /locus_tag="PLL59_03035"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HMY22809.1"
                     /translation="MEEIIDLKEKISELAQVGFLDINIDPTIDLFAEIDKLKKEKNAV
                     ILAHYYQEADIQDIADYIGDSLQLAQKAKETTADIILFAGVHFMAETAKILNPTKKVI
                     LPDLKAGCSLADSCPPEDFAVFKKKFPGYAVVSYINCSAEIKALSDFICTSSNAEFIV
                     NSIPKEQGIIFAPDRNLGAYLAKKLNREMVLWDGSCMVHEIFSLDKIRTLKEKHPHAK
                     MIAHPECEAAVLEIADFIGSTTGLLKYTQTDDAQEYIVATETGILHKMQQASPHKTFI
                     PAPPNNSCACNDCPHMKLNTLEKIYLCLKYELPELLMDENLRVQAKIPIDRMLEISAA
                     AGLKG"
     gene            complement(24554..25126)
                     /gene="nadD"
                     /locus_tag="PLL59_03040"
     CDS             complement(24554..25126)
                     /gene="nadD"
                     /locus_tag="PLL59_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HMY22810.1"
                     /translation="MNKIGLYFGSFNPIHIGHLLVATHIKEAANFDEIWFVVSPHNPF
                     KQKNELADENHRINMAKFAIENIPYFKVEDIELHLPQPSYTHRTLEELVKRYPDYEFN
                     IIIGEDNVAKLQEWKEIEWIKKHFYIIVYNRTNKTATKTVQNTLKYYELPLIDISSTE
                     IRKRIQENKQFRFFVPDAVEQYITFHKLYS"
     gene            complement(25119..26171)
                     /locus_tag="PLL59_03045"
     CDS             complement(25119..26171)
                     /locus_tag="PLL59_03045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMY22811.1"
                     /translation="MKKILHINTNMVWRGGEQQVYYIINHQSSQYQHFLFCRENSELY
                     HRNKSITTTTFTMRFGIDITPVFSLLKICKKNEIDIIHVHDSKALTLYIFATFLGLKI
                     PVVVHRRVNFPITNKYKYKHQKIKKIICLSDAVSKNFDNIVPKNKIVCIPPAIDINKY
                     NGEKNNRLKEELQLNEQSKIIGIVAAIEKEKNIEGFIQIANLLLSSNDELHFVIIGDG
                     SLFNEYQKKYSNNNIHFLGFRNNIAQLLSAFDVFVFSSINEGFGQILLEAMALGVPIV
                     CNNFPVAKEIIQSGKNGFIYTSTQEAVEQINNILNNTNQQEEIIATSKKYVQQFDVTL
                     INKKIEQVYQSILNNE"
     gene            complement(26168..27379)
                     /locus_tag="PLL59_03050"
     CDS             complement(26168..27379)
                     /locus_tag="PLL59_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22812.1"
                     /translation="MIKKNTSAFDILVKSALITLVIGLFFSNALKSIGLVVLALSSVF
                     LVKRKKDIILSIKHPLVLSTICIVIIYCVSAMLSSNKLYAWISIKNKLAIVFAIIAIM
                     QCKFDKNEIKYFLFVIIMCALLQSIYASYFYFNIDEWNNMYHVGNVLPTIKIHHVQIG
                     VLIAITILILYHFILKEKNKIHKIAMLIAGIFLFIFLHIFAVRTGIVLLYFMLLFYTI
                     YKCIIEKKWKYIVGIITVLSISSISALQFSDTLKSKLEYMKYDISQFRMNSENMHEYS
                     DARRLLSIKNGIEIWKNNWYLGCGIGDIYDECKKINQIKNPSVNEDLYYLPHSQYLYF
                     YSVFGIILGSIVILCFIFPAFYFIYKKNNLFSSIYVALLIFSIWDAYFGTLFGVCIYT
                     VVIAIGSKKTI"
     gene            27452..28024
                     /locus_tag="PLL59_03055"
     CDS             27452..28024
                     /locus_tag="PLL59_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMY22813.1"
                     /translation="MSTEPSLDKEQLFEQELLPHADALYNFAYHLTYNEEDANDLVQE
                     TFMKAFRFINSFDSGTNAKAWLFKILKNGFINEYRKKKKEPNKVDYEDVLAYQDADED
                     KGAVVFDLREDIFDDMIGDEITIALNKLPIDFKTVILLCDIEGFSYEEISKIIDIPIG
                     TVRSRLHRARNMLKESLSAYASKMGYKKSE"
     gene            28026..28271
                     /locus_tag="PLL59_03060"
     CDS             28026..28271
                     /locus_tag="PLL59_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22814.1"
                     /translation="MNHIRPPHNCNELVKDISLLLDGELDRHTENMLREEIEKCDVCN
                     QYYKSHAAYKMNVSQKITRMSCGENFKDSLRSKIRGL"
     gene            complement(28274..28813)
                     /locus_tag="PLL59_03065"
     CDS             complement(28274..28813)
                     /locus_tag="PLL59_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22815.1"
                     /translation="MVGKNNYWVLILIIIVLSNSKKSYAQEYRGNVGARFGYGIGITG
                     TYNLQNNQAIEFLLRYGYHGVVINKPGGNFQALYQKHWEIRRSNFTVFVGAGPSIGAG
                     KKNNISKQVYFALGISPQIGFDYTARRTKLPLIIALDYKPTFNVDFPLKKKISKKVET
                     DFSYYEIAFSIKFGLKRIR"
     gene            complement(28827..29576)
                     /locus_tag="PLL59_03070"
     CDS             complement(28827..29576)
                     /locus_tag="PLL59_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HMY22816.1"
                     /translation="MSAPFLIHLPQFEGPFDLLLFFIERDELSINDIPIYKITNDFLD
                     YIQHLERLNIEVASEFILVAATLMRIKAKMLLPRKDLDDAGNEIDPREELVQALLEYK
                     RFKEIVEILKQFEDHRALKYSRGNQKNEVEQLVKLLADDAELETVTLFRLLKAYQRSM
                     DKYKTKNEKIVHTVSPFKYSIEEEKDRLASFIKLKTKASFEEIFTTCESKIQAVFIFL
                     GMLELIQQQIISIIIGEGSNNFWLTTDVVEY"
     gene            29614..30894
                     /locus_tag="PLL59_03075"
     CDS             29614..30894
                     /locus_tag="PLL59_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22817.1"
                     /translation="MIVIYTPKVTNRIKYTFDFVFSQYFGIDYTIVHEQITAFVSSNI
                     YINYSKEIKQQYFNIVQHDLLLETHIAPQELFVSRVTNFSVFFKQDNRFDLSFDIFSS
                     IFYLIARYEEYLPHQQDTHGRYLSSNSILAHADFRFAPVVEYWLLYFKQRLEEQFKFL
                     NFKKYHFEYLPTFDIDNAYKFKGRNWFQHFPNFLTSDFWKYICHISKDKYDVYDDIFK
                     LLKETNSSSIFFFLLNDEGKLNSKVSPRADALQQLINTCKERATIGIHPSYLAPTQTV
                     LFDNELDILATYTGRIAVVSRQHFLRVSFPHYYRELSNTVIQTDYSLAYPDVPGFRAG
                     CSRPFYFFDIELDTQTHLKIQASCWMDATYAYYQTLDAEQINLNFLTLFNQLIEISGI
                     LVPIFHNDLLASEKYWGVFKFINKLATSRDELKI"
     gene            30878..31912
                     /locus_tag="PLL59_03080"
     CDS             30878..31912
                     /locus_tag="PLL59_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMY22818.1"
                     /translation="MSLKFKSGLILFGIILIFTFQTNAQDYKFSQFYNSPLNLNPAFT
                     GKINSLYRFVANYRVQYLPLQSPSPYNTLSASADFGVLRDKLKGDIFGVGVVFTNDRQ
                     TAIRTNTMMFSLAYHKSLGKQKNHYLSVGAQVGFLQRSVDLGNLAFASQFTGTGFDLS
                     LNNQENFAGEKYFKPNLNIGLFWSSNFTKSIGAYAGVSVFNIIKPKDSFFKSDNERAM
                     RFNTHAGLFIDVKKIVLISPNGMYMYQAKAQQWIAGSSFSFNVSGKSEPYKTAVSLGV
                     WYDGNGAVIASTGVSFLGLQIGFSYDATVKKDLNKAVKSVGALEASIIYTGRPLDKKK
                     SYSPLLCPKF"
     gene            32490..34352
                     /gene="mnmG"
                     /locus_tag="PLL59_03085"
     CDS             32490..34352
                     /gene="mnmG"
                     /locus_tag="PLL59_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HMY22819.1"
                     /translation="MFQSYDVIVVGAGHAGCEAAVAAAKMGSKVLLATMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDAMGGYSGIVSDKTMIQFRMLNQSKGPAMWSPRVQSDRMQF
                     HLVWREMLENTANIDFWQDMVSNLLVENGSVCGIRTGMGMEIRSKAVVLTNGTFLNGI
                     IHIGEKQFGGGRAGEKASKGITEQLVSLGFESGRMKTGTPPRIDGRSLDYAKMEIQNG
                     DDNIVGFSYKNIEKPKEQRCCWITYTNDKVHEILKTGFEKSPMFQGRIQGLGPRYCPS
                     IEDKINRFADKDRHQIFVEPEGWNTVEIYVNGFSTSLPEDVQYKALTSIPGFEKVKMF
                     RPGYAIEYDYFPPTQLALSLETRLIKNLFFAGQINGTTGYEEAAAQGMMAGINAHRAI
                     HDEAPVVLKRSEAYIGVLIDDLVNKGTEEPYRMFTSRAEFRILLRQDNADLRLTELSN
                     QIGFAEQERMDRVQLKKQASEEIIQFIKEYSVEPAEVDTFLESRGTSALIHKMKLEKI
                     VSRPQIGILDLIANVPSIKKHLDKYEIEFLEQAEIAIKYDGYIAKEYDQVKKMDKIEN
                     VILKPDFDYMSLPSLSMEARQKLSKMKPQTLGQASRISGVSPADISVLMVYLGR"
     gene            34381..36132
                     /locus_tag="PLL59_03090"
     CDS             34381..36132
                     /locus_tag="PLL59_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMY22820.1"
                     /translation="MHQHHYKVKSKLYYYIIVLFQCLILSCAQITAPTGGERDTKAPE
                     IVSISPANKSTNFNNKSIQIVFNEWIQALNNPKSQVIISPNIEPFPVIDIARNDMKIK
                     IKDTLQSNTTYSIFFGDNIKDNNEGNPLPNFTYIFSTGNFIDSLSLKGNINTKLDKFP
                     DNTFLLLYKEMEDSVFTKKRPFYITKIKSDGYFSLENIKEGQYKIFALSDKNNNYYYD
                     LPTEEIAFSDSMTTINANVDSLSLELFLAEEDKLRIIEFDRTLKNGICTVLFNKELSP
                     NKDDITATVVDGSKIDAIAFQDKTAKKLQIYFPKLEKDTNNLIVVLKNNAILIDTLRV
                     QSYQINAKQTINFFTDSTVVPLIETQALQLHAAYYSLSNIDTSKIQIRDSSNTSIPFS
                     ITRKDDLQTYIISALWKPGMRYKLSLQDSVIADLVGNFNKTQEFSFSPMSAKKAGNLL
                     ITYELPQNNVNYIVFLKNSAGKVLDKQIIRDSQRVQINYGLTLAGTYFIEVVEDSNNN
                     GIWNSGSFINKTLPEKIYKEAKPIIIKENWDAEETIKVDFSASKSVGIQNITSPQNTP
                     AFNSPNKTNKGATNTKQ"
BASE COUNT        12165 a         5986 c         6100 g        12011 t
ORIGIN      
        1 atggaaaaaa ccttgataga aaattgaaaa aaataagtta ctaagattcc cactttcgtg
       61 ggaatgacaa taacatatta taatgaaatt acttgtgcgg tttgatttag attgtatttt
      121 tttcactaat tgtcctaaca agcatattgt atcactttat ttcttagcaa aaaaagtaaa
      181 aacgaattgt catattacaa aaatgaatgt cgtttgcgtc tattaattgc tcaattcgtt
      241 tttcataaag tccatcaact cttttacata ttctctagag aagttaaatt gaatgcctgc
      301 tgttttatac atctctggaa ttgattttgt atagcctaat tgtaatgcat ttttataatt
      361 gtcaattgcc acagccttat tttgcttata gtttcgccat actgcaattg cacctaattg
      421 agcaatgcca tattcaatgt agtaaaatgg cacttcaaaa atatgcaatt gtttgtgcca
      481 taatattttt ctgaaatctt catataaatt ccaatctaca atgtctgcac ttaattcttt
      541 gtgtaattcc aaccatttat tttcacgttc tgtgcttgta tgattagggt tggtatatat
      601 ccaatgttgg aatttgtcga ttaatgctat ccatggcaac atcgtgataa tgccttctat
      661 atgtgtttcg atagcacgtt ttttatcggc atcattataa aatgcactta agccatcaaa
      721 ggtcataagc tccatgctca tcgaagccaa ttctgccatt tccgacgaaa tttcttgatc
      781 gaaattatac ggcaatgtgc gcattaaaaa ggaatgcacg gcatggcctg cttcatgcac
      841 cattgtttcc aaatcgcgat gtgtgcctgc ggcattcata aaaatgaagg gcacgccaat
      901 ttctggtaag ctcatgttgt agccaccagg tgctttgcct ttacgcgagt ttaaatccaa
      961 ataatgcata ttatccataa tagcaataca ctcggcgaaa taatcatcta cttgcgttaa
     1021 acatttgaca gatttttcga taagctcatc ttgcgtggaa aatggttgca aggcaggcag
     1081 gttggctaca tcgacttcta aatcataagg aaataattgt tcaacttgta gtgcttgctt
     1141 tcgttttgca taaatttcat ttaccaatgg aattacttct gatttgatag ctttgtgaaa
     1201 ttgctcacaa gcatgcacat cataatcaaa tcggcctaac tcggcaaaca taaaatctcg
     1261 atagtttgta aagccagcat ttaatgccat ttgatgacga attttcagca attcatcaaa
     1321 caaattgttc aacttttctt tgtcttgaat gcgtctgttg tttacttttt caaaaattga
     1381 ttttcgcaaa tttctgtctt gttgttgcaa aaaaacgcct gcttgcggca aggtaaattc
     1441 tttgccttcg tgctcaatgg tcattgcgcc aatagtggca ccatactctt gagacaagag
     1501 ttgtacttgt tgtgaaagtg gaatattttc ctctctaaat aattgtaatt gtgcttgaag
     1561 gtgacgcagg taaacaaaaa atcgctcttg gtctaaatcc tttacgaatg cagatgtagc
     1621 cagttttttg tttaattcat tgccgatgct catccagtta ggcattatgg tttgaatgaa
     1681 atcttcgtat gcatctttaa acgtttcatt gtctgtatca caggtttggc gaatatatcg
     1741 ccagcgatat tcttcatcaa caactgcatc tgtttcactg cggtctgcca gccactgcaa
     1801 tactgcctct ttgctttcga ttggtcgttg ttgtagttcc tggtaaaatg attcgatatt
     1861 ttgccagcca tgcaccacca aattttctgg caaatagttt ctttttttgc gagtcactgt
     1921 tttcatacta tttattaaaa ttaaaaagac ttatcaagca agtgtacttg ataagtctaa
     1981 caattatgta atagattaca ctttattttt tgtcatgcgt ggttttgcca cagattgttg
     2041 ttgcacattc atgtcttttg ctggtgcatg atgtttttga gtcgttgttt ttacactaga
     2101 tttgttttcc tcaccttcag cagatttttt gaaaatatca aatccttcga gtgcattata
     2161 ccatttcacc aattttttaa tatcagaaat gtttacgcga tctctgtcaa acgtaggcaa
     2221 tatttttcct aagtaggcac gcaaatcgtc gttcgatgca tttgcatcaa ctaaaggagt
     2281 gctatctttt tgatttttca tttcgatgaa aacatctaac aacaacacgg tatcgtcttc
     2341 tgtatagatt gaaatatttt ctaaaggtga aaacatgtgc tgacgattag aatagaattt
     2401 tttattagaa tcatctaatt cactcaatat caaaccatcg tttctgtttg ccactaattt
     2461 ttttaaacct gccactcctg taacggcaat aatttctctg aattccattt ttacttttta
     2521 tgtggtaaaa gtaagaaatg ttatggaatg acagaaaaaa aagatgttaa tagaaaagat
     2581 tattgtgtaa tggtaatttt tcctgaatat acaatgttct cattgttttg ctcataaaac
     2641 attttcacaa aataatttcc tgcagcaagt gttgtgttta tagtaataaa atctgttgga
     2701 taggcaactg cttgttgaaa tatttgctga ccaatgaggt catatatttc gactttccag
     2761 ttttgataag atgcactttt cggaattgga attgagaatt gaccattaga aggattaggg
     2821 aaaatagcta gtttttcctg cattaaattt tgcttgatat gagtcgtgcg attgcaatta
     2881 agccatggat aaataaagtg cgctatatga cgttctgtgc tatctaaaat gggttggttg
     2941 atgaagttaa aaatgatgtc tggaaaagtt aaattcataa atggcacatg tcctctgcct
     3001 atccaatctt cgaactctac gcgaatgcct ttgcgcagca ttactggata ttgagctttg
     3061 ccaccaaata aaactggcaa tgtaggaata tggaatggat tgccatagtt gtagggcacg
     3121 atttcatctg ctgagccatg aataatgagc atatcaatgc catttttgtt tatccaattc
     3181 gtatctaata tggcaccaga aacactaata caagcttttg gtttcaccaa atcaaattta
     3241 tggcgtaaaa tataatcagc gctatcgcca tttgcttcaa caatccattg ttgatattgt
     3301 gctggtaatt gctgcaagct gtctaaataa ctaataaaca tgctggaaac agctcctgcg
     3361 gaaacaccac caataaatgc acgtgaagtg tcgatacgat atggattacc attttgatag
     3421 gtcagcatca aatggtccac tgcttctttg gtgtctatca aggcacgaga cactgctttt
     3481 accattactt cctcagagag caagcctaat aaattaggct cttgtctata atcaatcgaa
     3541 acggtaacat agccacgttt agcgaaatag tcgcacatca acacaatatc cggactatta
     3601 tggtctaata atttcaggta agcaccgcca tgaatcagca acataattgg acgttgtgcc
     3661 aatgtatcgc cgtgcggttg atacacgtca tattttaaat cgatatattg accatcactt
     3721 tgtttcttac gtgcatattt aacgtctttt gtaatatcta ctctattaaa tacttgctga
     3781 atatatctgc cgttattaca atcatctgca tacatattga ctgtaatact tgtaaaaaat
     3841 aaaacaagaa gtaaaaacgg atttttcatg tcaataaatt tcaattaatg tacaatattt
     3901 attttgctcg tataaatata ttcttcttcg tctttctggt aagccaattt tataaaatat
     3961 gcaccatttg gcagatgttt atctataaaa acattttctg taaatacagg aaaattggtt
     4021 tggtacacat tttttccgct gatatcatac atatcaattg ttccattgtg gtattttgct
     4081 tcgaatggaa ttttaatttg gaagttacca tttgaaggat ttggaaaaat cgaaatgggc
     4141 gaatccataa ggtgttgttt gataccagta gttaaggcat cacacgttaa tagactatag
     4201 caaaaatttg ttatgtgacg cttggtgcta tccaacacgt atggattaaa aactacagct
     4261 aatggatttt gtccaaagag tgcacctaag tttaagcctc caacaaatgg aacatgatta
     4321 tagttgaccc atgtttctaa ctcagaacgt aagcctaaat gtttatatcg tttatctatt
     4381 aaagagctgc ccattaaatt cggaagcatt ggaagtccaa acggatgtcc ataactatat
     4441 ggaattacca catcaaccgt gccatgctgc gacagtaatg ctggatattg tctgtttggt
     4501 tttatccatg cagtatcgag tacggcacca gaaacattaa tcattcccaa tatattggca
     4561 ccacagaatt tatcatcgag taattgttgt gcattaggta ccacttgttc tatccattgt
     4621 ctatattgat taggcatttg gctaatgctg tccaaaaaca tgccatgtaa aaagcttact
     4681 gttcctgctg aaacgccacc aataaaaaca gattgtaagt ctatcctata cggattatta
     4741 tagtgcaatg ttgtatccac gattctgcaa aatgccaatc gagcgtccat cacggcacga
     4801 ccaaccgctt ttatcatatt ttcctcagat agtaaagaca ataagttggt ctctaagcga
     4861 tagcctatag aaataacgac atatcctttt tgtgttaaat ctaaagccaa ctctacgata
     4921 tcaggacttt ttttatctaa taatggaatt tccaaataag caccaccgtg cgccaatacc
     4981 accaatggtc gattttgagc agtgtcattt tttggttggt aaacatcgta tttataatca
     5041 agccaagcac catcacttct ttgtaacttt gcatatttga catcgctggt aatggtaaca
     5101 tcttggaaaa ttttttgttt atatctacca ttaaaacaat cggtggcgaa aatacttgat
     5161 tgaaaggcta gaaataataa aagcgtgtaa aaaattctat acatctgaat tgtttatcta
     5221 ttaaaattaa tgctattttt taaattattt attgctactt atcgaccaat gtgtgttctt
     5281 aacttttgta tattttatga ttttaataaa caaaatctgt gttaaaacag aatagcacat
     5341 tctgtatatt tgtatatgcc ttttaaaata cactcgtctt atcaaccatc aggagatcaa
     5401 ccatcagcta ttgaacagtt gagtgaaggc gttttaaaag gcgaaaaata tcaaacactg
     5461 ttaggtgtaa ctggctccgg caaaactttt accattgcca atgtgattga aaaaacacaa
     5521 agacctactt taatattatc acataataaa acgttagtag cacaacttta tacagagttt
     5581 aaagagtttt ttccagacaa caaggtagaa ttttttacct cgtattacga ttattatcag
     5641 ccagaagctt atgttgccgt tacggataca tacatcgaaa aagatttatc tatcaatgca
     5701 gaaatagaaa aattgcgact cagcacaacc acatcattaa tgagtggcag gcgagatgtt
     5761 atcgtagtgg cgtcagtgtc gtgtatatat ggtttgggaa atccagctga atataaaaat
     5821 cagattatac gcattagcaa aggacaaaca ctaagcagaa atactttttt atttgattta
     5881 gtaagtattt tatatgcacg cacgacagca gattttcagc gtggtaattt tagagtgtta
     5941 ggagacaatg tagaagtatt tcttgcctat gctgattatg catatcgaat tactttttgg
     6001 ggcgacgata ttgaagagtt ggaatccttt gaccctgtgt ctggaaagcg aatagaaaaa
     6061 atgcaagaca ttgctatctt tccagccaac atctatatcg caccacgcga ccaattgcca
     6121 actattattc atgaaataca agatgagctg acagcacatg tagaatattt caataaaaca
     6181 ggaaaacaac ttgagggaaa acgtttagaa gagcgtgtaa atttcgattt agaaatgatt
     6241 cgcgagctcg gctattgcag tggtattgaa aactattctc gattttttga tcgcagaaat
     6301 cctggcgata gaccattttg tttgttagat tattttcctg atgacttcct gcttgtagtg
     6361 gacgaaagcc atgttactat accacaaatt ggtggtatgt atggtggcga tagaagcaga
     6421 aaacagaatt tggtggagta tggttttcgc ctgcctagtg ctatggataa tagaccttta
     6481 aattttggcg aatttgagtc tttattaaat caaatcgtat ttgtgtctgc aacgccagca
     6541 gattatgaat taatgaaaac acaaggcgaa tttgtagagc aagtcgttcg tcctactggc
     6601 ttattagatc caccaataga tgtacgtcca agtctaaatc aaatcgatga tttattagat
     6661 gaaatacaaa agcgtatctc gattaatgaa cgcatactcg tcactacgct tactaaaaga
     6721 atggcggaag agttagcaaa atatttaagc aaattgaata ttaaatgtaa atacttacac
     6781 agtgatgtgg acacgatgga gcgtgttgaa attattcaag gattgcgtat cggcgatttt
     6841 gatgtgttga ttggtgtaaa tctgttgcgg gaaggcttag atattccaga ggtttctctt
     6901 gtcgcaattt tagatgcaga caaagaagga tttctgcgca acgaacgttc gcttacacaa
     6961 actgcaggtc gtgccgccag aaacgcaaac ggtttagtaa ttttttatgc cgatgtgatg
     7021 acgaaatcta tgcagaaaac tatagatgaa acggagcgtc gtcgcgaaaa acaaattgct
     7081 tataatattc aacatcacat cacaccaaaa actgtgctca aaagcaaaga agaaattctt
     7141 caacgtgctt ccattttaga catcagacaa acggctgaaa cgaaaactga aaaaaaacat
     7201 agagaacacg aagaaagcat gatcattgct gctgaagaac aaggtgaata tttgtcgact
     7261 gcacaaattg aaaagaaaat tgcacagtta aaaaaagcga tgcagcgcgc ctctcaacaa
     7321 atggatttta tggaagctgc tcgtttaaga gatgaaatgt ttaaatggca gaaacaatta
     7381 taaaaaataa aataagcatg aacagaaaaa caatactcat cactggtgca aattctggat
     7441 taggatttga agcggcaaag caattggcga aacaaggaca tgaaattatt ttactttgtc
     7501 gaaataaaga gaaaggagaa atagctgttt ctgaaataaa atcttattct aataatcaaa
     7561 acatacactt atatactgct aaccttgctt cgcaaaaaag catagaaaca gttgctcaac
     7621 aaatcaaaaa agatattagc aagttagatg ttttgttaaa caatgcaggt ggcgttttta
     7681 ataattttca attgtcagaa gatggcatcg aaatgacaat tgccaataat cattttaatt
     7741 atttttggct gacatactat ttatttgatt tattgaaaaa cggaaaagac gcacgtatta
     7801 tcaatgttgc cagcgactca cattacagcg caaaaaaaat tgatatcgaa tcattttatc
     7861 aaaaaaaggg aaattacttt gtgttgagtg cttacgaaca aagtaaattg gcgaatgtat
     7921 tatttactta cacattggcg gaaaaagcaa atccttataa catcacggtg aatgcattgc
     7981 atcctggatt tgtgtacacg ccaataggcg agaaaaatgg aaataaattt tttggaatgg
     8041 tttggagtgc tgcttcaaaa ctatttggtt tgtcagtaga gaaaggtgcg cgctcacata
     8101 tttatttagc aagcagtgat gaagttaaag atatcagtgg aaaatatttt cataactgca
     8161 aagcgaaaaa atcttctagt atttcctatg ataaaaacct acaggcaatg ttatggaaag
     8221 aaagtgaacg taaaagtggt ttcacatttt tataaaatct tctgctattt ataaaaagca
     8281 aaacccgatg ttattgacat cgggtttatt tttttcatcg tttgttgacg attacttttt
     8341 tgctgctgct ttttttgctg ccgctttttt aggagctgct gctttcttag gagctgctgc
     8401 tttcttagga gctgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntctta
     8521 ggagctgctg ctttcttagg agctgccgct tttttaggag ctgccgcttt tttaggagct
     8581 gccgctttct taggagctgc tgctttctta ggagctgctg ctttcttagg agctgctgct
     8641 ttcttagctg gagctttttt tgctgccgct ttttttggag ctgctgcttt cttaggagct
     8701 gccgcttttt ttgctggagc ttttttcgct gctggctttg cgcttgctgt tttctttgcc
     8761 attgttttta tttttttttt gtttcatttt tatacagttg aaacttttct gttggtatat
     8821 tttatttttt ttatgcagat taaaacacac ctttaactat tctgcaatat aaaagtaata
     8881 cataaatcat ttcatgcaac acttgagtta ttcataaatg ttgataattg tggataacgt
     8941 aaaggtgcat tttatatgct aaacacgcaa ataaaaaata caattgaatt ctattttatt
     9001 taacgtgttt ttattttagt tgctataaaa aaatagaaag cctgaaaaac atattcgcca
     9061 aaacatcaat aaacactata tgtttgagtg attatttttt ttatcgtgtt ttatgatgca
     9121 aatgtttttt catgtaacaa aaaataaaat tgaataaata aaatttcatc tctcttaaga
     9181 aaaaaaaata cacttttaaa aatttatgct tttatgaatt gaatatggtg gataaaatat
     9241 ttgatgaaac gaaaaataaa tgtggattat ttttaaaaat agaaatgaaa atgaaaagtt
     9301 ttatttgttg ttggcgctgg aatagcattg aaaaattcaa aaaaaataat agactttttt
     9361 atgctgctcc attttcaatt ccttatcttt cgtgtgaaaa ttattgcttg atgaagaaac
     9421 ttatcgtgtt tatttttgta ggcgtcattg cttttattag tgcatgttct tcgtgtaata
     9481 atgaaaaaga aaaaacgaaa cctgttgaaa tagacaacat caaagaatct tataaaatgt
     9541 tgccgagcga acaacagtct gtcatcgatg ttgtgtatcg ctggaatgca gcacacgatt
     9601 ctacacaaat cgatagcctt gagcaatttt acaatgatcc tgttttcttt tttaaaaaac
     9661 gaattgctcg tgcttctgtg cttgcctcaa aaaaatataa aatctcacat gctgatactt
     9721 atcgagaaag aataatcgga cgcattggta tttatgcatc tgatactggc gattacaagt
     9781 gtgagttttt aaaattagtt accatcaaca gcaagcccaa tgtttatcat tcctatttaa
     9841 tttttaaaaa acaagcggat agcacttgga gaattattgt agagagcgac aaagaagcag
     9901 acctgcgtcc tgagttggtt tctttgttcg aaaaatttga agaaagtgaa agcacatcat
     9961 ctggcgactt taatggagat ggcgaagcag aagatttaat cgtattaaaa ccaacacaag
    10021 actcgagtgg aaattataca agcaaacaaa caaaaatttc ttttaccaat ttagatttgc
    10081 cagagattac tatacagaat tgcgttggtg taaatgtatt aaacgagaac gatgtcgatg
    10141 gtgatggtgc agatgatttt tctgtagtaa taaaaaaaga agatggcgat gttggcgatg
    10201 ttattttata cagctttaaa cgtggcaact ggcaagagtt ggcaaaattt aaagcaccac
    10261 aagaagaagt gttctcctcg cgacaacaat taatagagtt tgccggaaat ggtaatatca
    10321 aaattcgaag cgctgaaaaa acaatagata atagagatac attggtttta aaaacgattt
    10381 ctacttggta aagttttgaa gttattgcta atataatttg tcagctctat gtatattcca
    10441 tcttattaca aacagaaaga catcgacaaa actattgcat ttatgcaagc ttataatttt
    10501 gcgagtttaa tttctattca agacaatatt cctattgcta ctcatttacc ttttatcata
    10561 gaaaaagaaa gcgaggcatt ggtgttgtat tctcatttaa gcaaagcaaa tccacaatgg
    10621 aaaacatttt cggaaaataa tatactcgta attttttcag agccacatgc ttatatttcg
    10681 ccttcattat atagtggcaa acaagaagta cctacatgga actatgttgc cgtacatgct
    10741 tatggaaaaa tttcaatatt gccgagcgat gaagaaaagt tggccgtttt acataaacaa
    10801 atgcaatctt atgaatcgga atatattgca caatttaaag cattagacca tcaatatatt
    10861 caaaacttat tgcaaggtat tgtagcattt aaattggaaa taacagactt gcaagccaaa
    10921 gaaaagctct cgcaaaacaa aaacgatatc gacagaaaaa atgtaaaaca acatttatta
    10981 gagagtgatg acacgaataa acaagcactc ggcaatatga tggcggattg aatctgataa
    11041 ttatcataga aaatattgca taatcatctt aattttgcac tataaatatt gcatcgtgct
    11101 ttcatacgat ttggttgata aatacaatgt tcctgttcca agatacacca gctatccaac
    11161 agtaccttat tgggaagagc cgatgaacga tatagatgat tggaaaaatc aaattcaaaa
    11221 agcaattgct tgtaacaaaa caaaagaagg catcagcctt tatcttcatc tgccattttg
    11281 tgagcaattg tgtacttatt gtggttgtaa taaacgcatt accaaaaacc atcaggtaga
    11341 agataaatac atagcagcac ttttaacaga atgggaacaa tacaaagcca tatttggcga
    11401 cgcattatgc attcgcgaag tgcacttagg cggtggtacg cctacctttt ttagtccaaa
    11461 aaatttgtac ctattactaa gtgcattatt taaagatgtg cgcattcatc cagcacacga
    11521 attcagcttt gaaggccatc cgaacaacac cacatttgag cacttagaaa cattatataa
    11581 gcttggattt cgacgcgtaa gttttggtgt acaagacaca gatgcgaaag tgcaaattgc
    11641 catcaataga gtgcaacctt tttctaaaac aaaacaagtt acagaatggg cgcgcgaaat
    11701 tggttatgag tctgtcaatt ttgatttgat atatggttta ccatttcaaa ccgaagaaag
    11761 catcagaaaa acaatcgaag ctgtcattac acttctgcca aataggattg cattctacag
    11821 ctacgcacat gtgccttgga caaaaaaatc acaacgcgct tatgatgaaa atgatttgcc
    11881 taaaggaaaa gataaataca ctttatacga agtaggcaaa gatttatttt tgcaagctgg
    11941 ctacgccgat gtcggtatgg atcattttgc attggcaggc gaccaactgc ttatcagtaa
    12001 gcaaaatggc actttgcatc gaaactttat gggttacacc actacaaata cagaagtatt
    12061 aattggatta ggtgtttccg caattagcga tatatttttt ggctttcgac aaaacttaaa
    12121 aacggtagaa gaatattacg aagccataga aaaagataca ttgcccatta ttaaaggtat
    12181 caatgttaca gacgaagata tcgtgtatag aaaacacatc ttaaatattg catgtaaagg
    12241 gaaaaccgat tggacaaatg aaattgaact tactgacact atgataaaac acttagatgc
    12301 attacaaagt gatggactca ttacttggca aaatgcatca ttagaagtaa gcgacttagg
    12361 ttggagtttt ttgcgtaata tttgctccgt gtttgacaaa aaaatgaata gcacaaaaaa
    12421 tgattcgcct tctgatacac caaagtttag tcaggctgta taatttttag tattttcacc
    12481 tattatgtct tggcaacaca ctacttctat ttatcaaatt tatccacgct cattttatga
    12541 tagcaatggc gatggtatcg gcgacttaca aggcattatt caaaaattag actacataca
    12601 gcaattaggt tttgaaacca tttggatatc gccattttac aaatcgccac aagacgattt
    12661 tggttatgac attgctgatt attacaccat ttcaccagaa tatggcacac tgcaagatgc
    12721 tgaaatgctg atacacgaag tgcacaagcg cgacatgaaa attgtgtttg atatggtgat
    12781 gaaccacacc tctaatcaac atcattggtt tttagaatct aaaaaaaata agacaaaccc
    12841 aaaagcagat tggtacatct ggcgcgacca gcccaacaat tggaaaagca ttgttggccc
    12901 aaaggcttgg cattattgtg aggaaagaaa gcaatattat tttgcttcat ttttgccatt
    12961 tcaacctgac ttaaattatc gatgtcctga agtaaaaaat gccatgtttg atgtttgccg
    13021 tttttggctt agcaaaggtg tcgatggttt tcgtttagat atttttaatt gtattttaaa
    13081 agataaagat tttaagaaca atcctttttc tttatggcac gccattccga gtgaagatta
    13141 tcctggaggc aattttcaaa tcagaaaata ttcactcaat cttccagaaa attttatgct
    13201 cgcaaaagaa ctgcggcaag tagtggacga atttccagat agacttttat taggtgaagt
    13261 atttggaaaa cacgaattaa agaaaaaata tcttggtgaa aacaatgatg gtttacacct
    13321 catttttctg tttgatattg tcatgtttac attcacagct aattttttta gaaaaaaaat
    13381 tttagaattc gaacagcaat atccatcacc atatatgcct actgtcgtat ttagcaatca
    13441 cgatcaatta cgaagcataa agcgcattgg caacaagctg gaaaaagcaa aattattagc
    13501 attattgcaa tttactatgc gtgctgtacc aacagtatat aatggtgaag aaataggcat
    13561 gatggacgga aacatcgcca taaaagacgg aaaagatccg gttgcaaaag tatatagttg
    13621 ggtgcctcaa tttattgcag acagattgcc tgtaggtata aatagagaca attgcagaac
    13681 gccaatgcaa tggaatcata cagaaaaaaa tgctggattt tctacagcag atacaacttg
    13741 gctgcccgta aacacaacga ttgaaaacag aaatgtagca caacagcaag cagataaaaa
    13801 ttcattgtta cacaccttta aaaagctgat ttcactgcgc aaaaattggc gcgctttaca
    13861 tcgaggcaat atcgaaatta tttacactgg caataaaaac atattggcct acacgcgaac
    13921 tgttgaagag gaaaaattat tggtaatgat gaattttagc aattcgaaaa tcagtaataa
    13981 aattaatcag cctattaaag aaatcgtatt ttcgataaat aatatctcta ctttcgatgg
    14041 tgtattattg ccgtatgatg gtgtgatact gaaaatataa actatttatt acttttataa
    14101 aaattataca atgtcagcaa cacaagatgt ccatattatt gagaaatttc gtgagatgat
    14161 agggaagcgt tatgcatatc acgacttaat caatagattt gaaatacctg cgagcattac
    14221 agaagagatg ataaaggaaa tagaaaatta ttttctaacc attatctatc caccagcgcc
    14281 agagcgaaaa gaattagaag aagcatttaa agatttggca gcctatgtaa aacagcctcg
    14341 aaaaatatgg aatttatttg gcgacatggc acgagcgctt tttaaatttg gtcgtcattt
    14401 tatgacggcc ttaaaagcag gcatggacgc attggattct tttgttggcg ccaaaaattt
    14461 tgaaaattcg atggcgctca ttgctaataa aaatggcatt cggccgccta tgagcgatga
    14521 agactttgaa gacaccatgt atcaattacc acgcgaagag gtggaaaaat ttatcaaaga
    14581 tgtaaaaagc ctttttggtg caatggtaaa cacaacactc ttatctaaaa ctttggatat
    14641 attagaccat gtcattcata cgatggaaaa aaaaccgaat gtttttccgc aaaaagaagt
    14701 agaaggcatt aagttgggaa aatccttgtt gcaaaaaggc tatgatttat tctctaaata
    14761 tgatgatact actaaaaatc ttatcgtcga tattatttat aaaaatgaaa tgtggtatgt
    14821 ggattatgtc tataaaaaga aagaaggaaa ataaacaata attaagaaag tttcacattt
    14881 gcgcaagcac gattttagta tttttaaaag ttgatatggc acgtaaaatt atatatactg
    14941 acgacgcacc agagcctatt ggcccttact cacaagctgt ttacattaat ggtctgttgt
    15001 tcgtttctgg acaaattcca ttagacccgt tttctggcgt attaattcaa gatggaattg
    15061 aagaggaaac cgaacaagtg atgcaaaatt taaaagccat cttacatgct gcggaaatgg
    15121 atttttcgaa tgtagtaaaa tgctctattt tcttgacaga tatgcgcgat tttccaaaag
    15181 taaatgaagt gtatggaaaa tattttaaat cgcaagcacc agcaagagaa accgtggaag
    15241 taagtgtatt gccaaaaaat gcacatgtcg aaatttcatg tatagcagct cgataattta
    15301 ctaaaaaagt aaacacatca tgaagcaact gattttaatt attatatgta tcgcaagcat
    15361 aaagcagacc cttacagcaa gcgaaaatat tgaaatttgc aatgctgcaa aagacagcgc
    15421 agcagcttta tttgagcgtg gtggcttagg attttatgca tcttcaaaaa tgaatcctga
    15481 tttatataat tatattatta tcaccaaaga aaattataag aagacgacag ttagaatttc
    15541 aaaaagcaca agcgacacgg caaagttgat gaagtataaa ttgtttagca cttcgtatga
    15601 taaaaatgcg gataaatcgc aaccattaga cactagtctc gattataaaa agtgttataa
    15661 tgctgcattc caagctaagt tagactccgt atttaaatgt gattttttta gaaaatcaga
    15721 ttctattctg aaggcttatg ataaagccgg caaaggctac agtgctgtag agtttaaagg
    15781 tggtccgagt gccttgcaaa aatatttaga caaaacgata gtgtttccta aagactgtaa
    15841 gccgaatgac agcgataagg cttatcgtgt ttattactcg ttttttgtag atgaaaacgg
    15901 tagcatttct gacatcaatt taatcaaatc gaattgtaaa gcttgcgaag caagtatctt
    15961 agaagccatc aaaaaaatgc ctgcttttgt gcctgcactc gaagctggaa aacccaagaa
    16021 aagcaaatac atcttgcctt atttaaaagc atatactaag ccaaaaggct agttggtagt
    16081 aagcatacag agaaagcata gaaatgaaaa ttgaagaaat aacatttgac agcgatgaca
    16141 aaaatctttt tttaattgac gatatccaac taaaggcaga cgcaattaga tattcaattt
    16201 tacccaagtt agaggttata aacaacgaat taatttcacg aattaccgaa atgtatgatt
    16261 ttgatttatt tcaaaattgt gccataggta aaacgccaca attcaggact tcaaaaagtc
    16321 aaagaacaaa gcctgtaacc ttggattaca aacactcaat tatttggatt aacggacagc
    16381 gaacaaaaaa caaatggaaa ggattagaac gtataaacaa taaagaagct caaataacac
    16441 caactgcaat cggaattcgt ttaacaacag aaggtatatc atcattattt tggtttggat
    16501 atgtaaaaaa tttcacggaa gaaacttata gaaaattcta tgactttttg gacagagaaa
    16561 taattaacgt attatctcta ttaaaccgtt ctggctgtaa gtttcaaaga tggtattttg
    16621 aaattattga tataagaaag acattacagc aaaaatttga aaatggcgat tataatattt
    16681 taatcggaca gcctaaagca ttcgaatacc ctataaacta tgaaaaaata aacgaaatta
    16741 ttttatctaa tctaatgcta tttcctttat tcaatgcttt tctaaacata tcattgggca
    16801 tagaacctaa tattaaaaat gacatagaaa aagccgaaaa gaactttcaa agctattatg
    16861 aaaaatattt tacagaaaaa caagaagaaa ctagtgtttt gtcagaaagc gatatgcaga
    16921 catttagaga aaaagccgac aataaaataa aagtgcaagc aggtatcaga tggcaagttt
    16981 ttaaaagaga taattggaaa tgtgttgctt gtggcagaag tgcagaagaa aatattattt
    17041 tacatatcga tcatatttta cctcgctcaa aaggtggcaa agacgaaatg aataactacc
    17101 aaactctatg tgaaacttgt aacatcggaa aaagcaataa agatgagaca gacttgcgaa
    17161 aaaaataatg cctactacca acatatcatt gcaaaaatgc ggtagtaatc tgccgcactt
    17221 cagcaatact taaccgtaat tactgatatt ttggcaataa tataaaatac aaaaaaaagt
    17281 cattcttttt aacagaatga ctttttttct tgcatggcaa tttttataaa tgcttattcg
    17341 ttaatttctt ttgatttgat gagtagtgat gctaaaatag agatggtgat tatcaccaaa
    17401 ataatggcga gcgaatgcac gatttctatt ttataaaaat cggcaataca cattttggtg
    17461 cctacaaata ctaatatcag cgacaagcca tattttaaga aacggaaata agcggtaatg
    17521 ccatcgagtg caaaatacaa ggagcgcaag cctaaaatgg caaacacgtt tgatgtataa
    17581 acgataaatg tatcattcgt gatggctaat attgctggaa tcgaatctgt cgcaaaaatt
    17641 aaatcggtaa attctattaa tagcaatacg ataaacagtg gtgtcgcatg tttaacggca
    17701 tcgatgctta caaaaaactt agcgccgtgc aaggtgttcg tgactggcat aaatttctta
    17761 aacaatctga ctaaaggatt tttatctggt tctaatggtt tttcgtcttg caccatcatt
    17821 ttaataccag taatgaccaa aaatgcgccg aacacataaa ttatccaatg aaatttagat
    17881 atgagcgcaa cgcctgcaaa tatgaatatt gcacgtaaaa ttaatgcgcc aataattccc
    17941 caaaaaagaa ttttatgctg atactttttg ggaatattaa aatatgaaaa caccattacg
    18001 aatacgaaaa tattatcgac actcaatgct ttttcgataa catatcctgt aaaaaattct
    18061 atggcttttt cttttccgaa ggagtaatac acaccaatat taaacaacat ggcgaagcac
    18121 acccaaactg cgctccatgc cagcgcttct ttaattttta ctgcatgtga ttttttatga
    18181 aacacaccta agtcaagtgc caacataata catacaaaaa ggttaaatcc tatccaatac
    18241 caaatactta cttccattta ttaatttttt aaagaaattt cgggcgagct tcgctcgccc
    18301 gaacacacac tatgaaaaaa aataatgctt ttttgtaaaa gcataactgt ttattatctt
    18361 ataattcatt aaactaattc tggtggaaag tctaaagtgt tagtataatc gcctgttgga
    18421 aaatgatgtg ctgtctgcgt ataattaaac cagcctattt cctcataatc ttgttttgat
    18481 gtttggtgaa caaattgaaa ttgttctgtt tctaatacgg cttgtagctt tgtgctttcc
    18541 tcttgtagct cgtgaaaaat tgtcgtttta ttattattta ataaggtgtc atttgactca
    18601 tttgtacaca caaaaaagaa aattaacagt aaatacgcca ccactttaga cattacacat
    18661 tgcattgtat aattgagtaa cactacttct cttgaaaaag tttccatcga tacctttttt
    18721 ttatcttatt tttgataatc agaaacaata ctatgcaggc tacttttaac gaacgattca
    18781 gacaggttgt cttatttgtc atcttattgg cattagccat acttattttt attcaaatta
    18841 attttgtggt gcctggttta ttaggtgcgc tcactttgta tttactcaat agaaaattat
    18901 tgtttacact cagctttcag aagaagtgga atatatacat cgctgcgagt ttgctcttgt
    18961 tgattgatgt catcattctg gccattcctt tcggattagc aattttattt gtagtgccta
    19021 agttgcaaaa ttttagtagt aatgcacaag aattatttca aggtgtgaag gaaattgtag
    19081 tgcgcattga tgaagctacc ggaatagaat tattgtcaaa ggaaaatttg agcaatattc
    19141 ctgctattgt ttcatcacat ttaccagatt ttttgagcac aactgcttct ttgtttttta
    19201 acttaagcac tatgtttttt gttttgtttt ttatgctaat caatgcgaca aaaattgaat
    19261 cttctttgat aaaatatata ccactcaaac ccgagaacaa acaaaaaatt acggcagaaa
    19321 caaaaaatat tgtaacatca tacgctattg gtattcctat attagctatt gcgcaaggct
    19381 tttgcgctta tattggttac aatattttta atattagcga cccgatgctt tggggttttg
    19441 ttacctgtgt atgttctgtg attccatttg ttggttctgg cttaatttgg attccgctgg
    19501 tcgcctattt atttgttggc ggtcatgaaa gtgatgccat tggcttagcc atatattgtg
    19561 ctattgttgt tttaaatgtg gacaatgtgc ttcgtttatt tttattaaaa gcatttgcca
    19621 acatacatcc attaatcact ttgtttggtg tgataacagg cattaaatta ttcggtttta
    19681 tcggattaat ttttggaccc ttattggtgt cctatctatt gttgatgata aagatttatg
    19741 tcagcgaatt tacgagtagt gaagtcagtg aataaattca tacaacattt atttgcttgt
    19801 attttcacta tacaaacacg cttttaaaag ttcgtttact tctttgtgtt gaatgagcaa
    19861 catcgcatgc gaaccgcctt ggacaggaat ggcattttgt atattgcgat aaggaaacat
    19921 ttcgtctttg gtgccatgta tatgtagtat gtctttacga taatcttcat ttccttgcca
    19981 ttttacaata gcatttacac accaatataa aaatgaagta tcgttatttt ttgccatgct
    20041 tatcgctaat tttcgcatgg ctggttgctt taaaacttcg cgtttaaaca agctgccaaa
    20101 ttgtatagag ccaataaatc cgcgacctgg caacacttta tgtaggttgg tatattttaa
    20161 ttgttttaaa ataaatggca tttcttttct acattttaca gaagaaatta aaaccacttt
    20221 ttctgcctgt atgtgtttaa ttaattccat cgtcataatg ccacccatag aattgccaac
    20281 gatattaaat gcttgtgatg tatcgatgag cggaataaat tttttgacat acgtttccat
    20341 agtatcgtgt ttgtcgtgct ttggaaacgg caaatacaca gcattttcaa tagctgtcat
    20401 ttgatatctg aaaagtcgtt catcagatgc tataccacca ataaaatagg tttttgtgga
    20461 catgtacaaa tgtaatcaat atatgcgatg cttatacagg ttttgcttct ccttcatgtc
    20521 gctaccgcat aacaggttat aattagtggt tttgttactt gatttaacaa taattttaag
    20581 catgtcacaa atcctgattt ataaggattt tatttattcc aaatgtgacg caaatttaca
    20641 ttaacaaaaa cgcaaatata tagagacata aaaatcttag gctttaaaca aagaaacata
    20701 aaaacccaac ccaaagaaaa gaaacataaa catcctccac agaaacgtaa gaacccagag
    20761 agcagcgtca gctgctcttt ttatttttta ggcgtatgca attgtctttc gattctggta
    20821 tctggtatta accaaataat ggccactatt gtatagctga ttaaactaat gataggaaaa
    20881 taggtggcaa tggcaatagc aaaaagatag agtaacaagg aaatattttc tttgttttta
    20941 ctttttagtg cttttccaat aaccgaattt tctccttcgt gttttgccgc tgatttagct
    21001 aataaaataa aacttatcgc acatagaagc agatttatac cataaagcaa aactggaata
    21061 ctttgaaaat gattgtcgcc catccacgat gtgctaaaag gcaacaatga aaggcaaaac
    21121 agcaatatta aattaagcca taataccttt ccgtttacat gttctatcgc ttgaaataaa
    21181 tggtgatgat tattccaata aataccgaca taaataaaac tgatgatata ggaaataaaa
    21241 ttaggcaata ctacttttaa tgctgaaaat gttgcaccga ttggcgcttt catatttaac
    21301 accattaccg taattataat tgcaatcaca ccatcactaa atgcttcgag tcttcctgtt
    21361 ttcaatgcaa tttttttgta aatataagtg tatgtgccta ttcttccaaa tagccaaatt
    21421 tacctttatt aaaatcatca aatgcctgta ttaattcttg tttagtgttc atcacaaatg
    21481 gtccgtgcgc tgcaataggt tcgccaatag gttcgccact caataccaat acgatggcat
    21541 ctgctgttgc tttaatagta aaagtttcgc cttcatttgc cattaataca aattggtctt
    21601 cttttacagg ttcactttca ttgactatta tttcaccttc tattactaat aatgccgtgt
    21661 tgaagtgttt tggaaaatcg aatgttgcca gcgcattttt atgtaattta gcattgaata
    21721 agtgtaaagg tgtaaacgta gaggcacttc ctttaatacc tttatattcg cctgcaatga
    21781 tttcaatttc gccaccctta tcttccaatt cataacgact cattgttgca ttttctatag
    21841 cttgatattt tggtgtgctc attttgtctt tggcaggtag atttacccaa agttgcacca
    21901 tctgaaaatc gccacctttt ttactccact ctctctcgtg aaattcttta tgcaagacac
    21961 cactagcagc agtcatccac tgcacatcac cttcaccaat aatgccaccg ccgccactac
    22021 tatcgtggtg ctctactttg cctttgtagg caatggtgac agtttcaaag cctctgtgtg
    22081 gatgcacgcc aacgcctctt ggtgtgtcgc ttggctcaaa ataaaatttg gaattataat
    22141 ccaacataat aataggatcc atgcgctgca tatctaaacg gtaagcactt ggaataaaat
    22201 tatgcactct aaatccatcg ccaacaaaat gtggctctct tggattaact actatttcta
    22261 ctggtttcgt tgccatattc ttttattttt atatggcaaa attaatacgc ttacttttct
    22321 tacacattga cctatggtaa gatttatttt tgaaattgct gtgctaattc tttgcgcact
    22381 ctactcaaac tttctggcgt gatacctaaa taggaggcaa tcatggtttg tggtacacgc
    22441 aaacttatat ctggatagac ttgaataaaa tctaaatagc gttgctctgc ggtggcgctt
    22501 aatagttgtg tgatccgttt ttgtaaatgt ctgatgtggt tgtgtaaaag tcgattatta
    22561 taatctaaaa aattgggatg tgataacgat aattcttgaa aaaacaattc gtctaataca
    22621 aacactttcg tgtcttctat ggcttgtata aaataattgg aaggattgtc gaaatatacg
    22681 ctttctctat cagaaatgaa ccatttttct ggcgcaaatt gtatgatgtg ttctttgcct
    22741 ttttcgtcta tggagtattg ttttaacagg cctgattcta caaaaaaaga atgcttacat
    22801 ttttctcctt gtcgcaacag aaaatcacct ttatatattt gctgctgttt acaatgtagc
    22861 attaaattat ctatgctatc attgtctaat gcaatattag aagtaagata agttttgaag
    22921 ttgaaactat tcatctgcaa taattaaaaa tactactata cttagtataa tgcttatgaa
    22981 ataacataaa aaaaaactaa cctttcaagc ctgcagctgc gctgatttct aacattctgt
    23041 cgataggaat ttttgcttga acacgtaagt tttcgtccat caataattct ggtagttcat
    23101 acttcaaaca taagtatatt ttttcgagtg tattgagctt catgtgtgga caatcattgc
    23161 aagcgcagct attattgggt ggcgctggaa taaatgtttt gtgtggcgaa gcttgttgca
    23221 ttttatgcaa gatacctgtt tcggttgcga caatatattc ttgcgcatca tcggtttgtg
    23281 tatatttaag caagcctgtg gtagagccta taaaatctgc tatttccaac acggcggctt
    23341 cgcactctgg atgtgctatc attttggcgt gtggatgctt ttctttcagc gtgcgaattt
    23401 tatctaatga aaaaatttcg tgcaccatac aagagccgtc ccacagaacc atttctctgt
    23461 ttaatttttt tgccaaataa gctcccaaat ttctatccgg cgcaaagatg atgccttgtt
    23521 cttttggtat cgaattgacg ataaattctg cattggagct ggtgcaaata aaatcgctca
    23581 gtgctttaat ttcggctgag caattgatat aagaaactac ggcgtagccg ggaaattttt
    23641 ttttgaaaac tgcaaaatct tctggcggac agctatctgc taaagagcaa cctgctttta
    23701 aatctggcaa tatgactttt ttcgtaggat tgaggatttt tgccgtttct gccataaaat
    23761 gcacacctgc aaacaggata atatcggctg ttgtttcctt ggctttttgt gctaattgta
    23821 aactatcgcc aatataatct gcaatatctt gaatatcagc ttcttgataa taatgcgcaa
    23881 gaataacggc atttttttct ttttttaatt tgtcaatttc ggcaaataag tcgattgtcg
    23941 gatcaatgtt gatatctaag aatccgacct gcgccagctc ggaaattttt tcttttaaat
    24001 ctattatttc ttccattttt ttaaatttca aaatttgatg tttcaaaggt aatttgattt
    24061 ttcataaaac taaaattttt taagattgtc cgtttttgaa acctttaata aataaatctt
    24121 tttaaaaaat tttattacta ttatttatgt gtgtgaattc gtggaaaatg atttttatca
    24181 aaatttatac tttattgatt ttttttgtag ctgttctatt tattaacatc ttttacacag
    24241 cttaaagttc ctattttatt agataattgc attttgattc acatttgtta gtacacttat
    24301 ccatgtgtta atgtgggtat tttttttctg ttgctttacg tttttattgt gaatagtatg
    24361 tgtttaaatt tatgaaaact aggcttgttg gtggagatta ttttacagta ctttttaaaa
    24421 gtgtggatac ttaattctat acacaacaat acacataaaa tttaattttc taacatcact
    24481 tcatgtggat aaaatagcac ataaaataag gcttttagcg cttgtactaa catggctgtg
    24541 tataactcaa attttaacta tataatttgt gaaacgtgat atattgttcc acagcatcag
    24601 gtacaaagaa acgaaattgc ttattttctt gaattcgttt tctaatttct gtagaggaaa
    24661 tatctattaa tggcaattca taatatttta atgtattctg cactgttttt gttgctgtct
    24721 tgtttgttct gttatatact attatataga agtgtttttt tatccattct atttctttcc
    24781 attcttgtaa ttttgctaca ttatcttcgc caataatgat gttaaattca taatcaggat
    24841 atcgtttgac taattcttcg agcgtgcgat gtgtataaga aggttgaggt aaatgaagtt
    24901 caatatcttc tactttgaaa tatggaatat tttcgatagc aaattttgcc atatttattc
    24961 tgtggttctc atctgctaat tcattttttt gtttaaacgg attatgtggt gaaactacaa
    25021 accatatttc atcaaaattg gcagcttctt taatatgtgt agcgactaat aaatggccaa
    25081 tatgaattgg attaaaggag ccgaaatata aacctatttt attcattgtt gagtattgat
    25141 tgatagactt gttctatctt tttattgatt aaagttacgt caaattgttg tacatatttt
    25201 tttgaagtcg caataatttc ttcttgttga tttgtattat ttaaaatatt attgatttgt
    25261 tctacagctt cttgtgtgga agtatagata aaaccatttt ttccagattg tataatttct
    25321 tttgccacag gaaaattatt acatactatt ggtacaccaa gcgccattgc ttctaataat
    25381 atttgtccaa agccttcgtt tatgctacta aacacaaata catcaaaggc tgataataat
    25441 tgtgctatgt tatttctaaa gcctaaaaaa tgtatattat tgttgctata ttttttttga
    25501 tattcattaa ataaacttcc atcgccaata attacaaaat gtaattcatc attactactt
    25561 agtagtaagt ttgcaatttg tataaaacct tcaatattct tttctttttc aatagcagca
    25621 acaatgccta taattttgct ttgctcattt aattgtaatt cttcttttaa tctgttattt
    25681 ttttctccat tatatttatt gatatctatt gccggaggaa tgcatacgat tttattttta
    25741 ggaactatat tgtcaaaatt tttactgaca gcatcactta aacaaattat tttttttatt
    25801 ttttgatgct tgtatttata tttattagta atcggaaaat tgactcttcg atgtacaaca
    25861 acaggaattt ttaaacccaa aaatgtagca aaaatatata gcgttaatgc tttactatcg
    25921 tgtacatgta taatatcaat ttcatttttt ttacaaattt taagtagtga aaaaactggt
    25981 gtgatatcaa taccaaatct cattgtgaaa gtagtggtag taatactttt atttcgatga
    26041 tataattcac tattttctct acaaaataag aaatgctgat attgcgaaga ttgatgattt
    26101 ataatataat acacttgctg ttcgccacca cgccacacca tatttgtatt gatatgtaat
    26161 atttttttca tatcgttttt ttactgccaa ttgctattac tacagtatag atacatacac
    26221 caaataaggt tccgaagtaa gcatcccaga tagaaaatat taataaggct acataaatac
    26281 tactgaataa attatttttt ttatagataa aataaaatgc tggaaatata aaacacagta
    26341 taacaatact acctaatatt attccaaaca cactgtaaaa atagaggtat tgactatgcg
    26401 gtaaatagta taaatcttca tttacacttg gattttttat ctgatttatt tttttgcatt
    26461 catcataaat atcaccaata ccgcaaccta aataccaatt atttttccat atttcaattc
    26521 cattttttat cgataataat cttcgtgcat ctgagtattc atgcatgttt tcactattca
    26581 ttctaaattg agaaatatca tatttcatat attccaactt gctttttaat gtatcagaaa
    26641 attgcaaggc actaatacta cttatactta aaacggtgat tattcccaca atatatttcc
    26701 attttttttc aatgatacat ttgtatattg tataaaatag taacatgaaa tacaacaaca
    26761 ctatacctgt gcgcacagca aaaatatgta agaaaataaa taagaaaatg ccagcgatga
    26821 gcattgctat tttatgaatt ttattttttt cttttaatat aaaatgatac aaaataagta
    26881 ttgtaattgc aatcaaaaca ccaatttgca catgatgtat ttttatagta ggcagtacat
    26941 ttccgacatg atacatgttg ttccattcat ctatattgaa ataaaaatag cttgcatata
    27001 tactttgtag taaggcacac ataataatga caaaaagaaa atattttatt tcatttttat
    27061 cgaacttaca ttgcatgatt gcaataattg caaatactat tgctagctta tttttaatac
    27121 ttatccaagc atataattta ttgctactca acattgcaga tacacagtaa ataataacga
    27181 tacaaattgt cgataatacc aatggatgtt ttatagacaa gatgatatct ttttttcgtt
    27241 tcacgagaaa cacactactc agtgctaata cgactaatcc gatactcttt aaagcattag
    27301 aaaaaaatag accaattact aaagtaatta aagccgattt taccagtata tcgaacgctg
    27361 atgtattttt ttttatcatg cagtctgtaa atggaactta ttctacaaat ttactgttat
    27421 ctacctaaat tttactacct ttatgacact cgtgtcaact gaaccatctt tagataaaga
    27481 acagctattt gagcaagaac ttttgccaca tgcagacgca ctttataatt tcgcttacca
    27541 tttaacatat aacgaagaag acgctaatga tttagtgcaa gaaacgttta tgaaagcatt
    27601 tagatttata aattcttttg actcgggaac taatgcaaaa gcttggttgt ttaaaatatt
    27661 gaagaatggt ttcattaacg aatatcgcaa gaagaagaaa gaacctaata aagtcgatta
    27721 tgaagatgtt ttagcatatc aagatgcaga tgaagataaa ggtgctgtag tttttgattt
    27781 gcgagaagat atttttgatg atatgatagg tgatgagatt accattgcac ttaataaatt
    27841 gcctatagat tttaagacgg ttattctatt gtgtgatatt gaaggatttt cgtatgaaga
    27901 aatatctaaa attatagata ttccaattgg aacagtgcgt tccagattac atagagcgcg
    27961 aaatatgctc aaagaaagct taagcgccta tgcatcaaaa atgggttata aaaaaagtga
    28021 atagtatgaa tcatatacga ccaccacaca actgtaacga attggtaaag gatatttctt
    28081 tattattaga cggtgaactg gacagacata cggaaaatat gttgcgagaa gaaatagaaa
    28141 aatgtgatgt atgtaaccag tattataaaa gtcatgcagc ttataaaatg aatgtatctc
    28201 agaagattac aagaatgagc tgtggcgaaa attttaaaga ctcacttcgc tccaaaatta
    28261 gaggtttata attttaacga attctcttca acccgaattt tatagaaaaa gcaatttcgt
    28321 aataagaaaa atcggtttct acttttttac taattttttt ctttaaagga aaatctacat
    28381 taaacgtagg tttataatct aatgcgataa tcaaaggcaa tttcgtacga cgtgccgtat
    28441 aatcgaaacc aatttgtggc gaaatgccta atgcgaaata cacttgctta ctaatattgt
    28501 tttttttgcc agcaccgata gaaggtccag cgccaacaaa aactgtaaaa ttacttctac
    28561 gaatttccca gtgtttttga tacaaagcct gaaaattgcc acctggttta ttgatcacca
    28621 caccatgata accataacgc aacagaaatt ctatggcttg attattttgc agattgtatg
    28681 ttcctgtaat gccaattcca tagccaaacc gagcaccgac attgcctcta tattcttgtg
    28741 catagctttt cttagaattg gacaaaacga taataatgag tattaaaacc cagtaattgt
    28801 tttttccgac cattcagtaa atgtaattaa tattctacaa cgtcggtagt gagccaaaaa
    28861 ttgttgctac cttcaccaat aataatagaa ataatttgtt gttgaattag ttcaagcatt
    28921 cctaagaaaa taaatacagc ttgaatcttg ctttcgcaag tcgtaaatat ttcttcaaat
    28981 gatgctttgg tttttaattt tataaaagag gcaagtctgt ctttttcttc ttcaatagaa
    29041 tatttaaaag gagaaacagt atgaacaatt ttttcatttt ttgttttata cttgtccatg
    29101 ctacgctggt aggccttcaa caatctaaac agggtgacag tttctaattc cgcatcatct
    29161 gctaataatt tcaccagttg ttcaacttca tttttttgat tgcctctcga atattttaaa
    29221 gctctgtgat cttcaaattg ttttagaatt tctacaattt ccttaaatcg cttatactct
    29281 aataaagctt gtactagttc ttcacgaggg tcaatttcat tgccagcatc gtcgaggtct
    29341 ttacgtggca acaacatttt tgctttaata cgcatgagtg ttgcagctac taaaataaat
    29401 tcactggcca cttcaatatt caatcgctct aaatgctgaa tataatctaa aaaatcattc
    29461 gtaattttat agattggaat atcattaatg cttaactcat ctctctcaat aaaaaagagt
    29521 agtaaatcga atggtccttc gaactgcggt aaatgtatga gaaatggcgc tgacatgata
    29581 tgtaaaaata caaatctgct taattaaacc atagtgatag ttatctacac acctaaagtt
    29641 accaacagaa ttaaatatac gtttgatttt gtttttagtc aatattttgg tatcgattat
    29701 accatcgtgc acgaacaaat tacagcgttt gtaagttcaa atatttatat caattattct
    29761 aaagaaatta aacagcaata tttcaatatt gttcagcatg atttattgct tgagacacac
    29821 attgcaccac aagaattgtt tgtttcccgt gtcactaatt tttcagtttt ttttaaacaa
    29881 gataatcgct ttgatctttc ctttgatatt ttttctagta tcttttattt aattgctcgc
    29941 tatgaagaat acctacctca tcaacaagat acgcatggca gatatctttc ctccaattct
    30001 attttagcac atgcagattt tcgctttgct ccagttgtag aatattggct cctttatttc
    30061 aaacaaagat tagaggaaca gtttaaattt ttgaatttta agaaatacca cttcgaatat
    30121 cttcccactt ttgatatcga taatgcctat aagtttaaag gaagaaattg gtttcaacat
    30181 tttccaaatt ttcttacttc agatttttgg aaatatatat gccacatcag taaagataaa
    30241 tatgacgttt atgatgacat ttttaaatta ttaaaagaaa caaattcgtc ttccattttt
    30301 ttctttctat tgaatgatga gggaaaattg aatagtaaag tttctccaag agctgatgca
    30361 ttgcaacaat taataaatac ttgtaaagag cgcgcaacca ttggtattca tccatcttat
    30421 cttgcaccaa cacaaacagt actttttgat aatgaattag acatccttgc aacctatact
    30481 ggtcgtatcg ccgtcgtttc cagacaacat tttttgcgtg tttcctttcc tcattattac
    30541 agagaattat ctaacacagt tattcagact gattattctt tggcatatcc agatgttcct
    30601 ggatttagag ctggttgtag ccgaccattt tattttttcg atatagaatt ggatacacaa
    30661 acgcatttaa aaatacaagc ttcttgttgg atggatgcca cttatgctta ctatcaaaca
    30721 cttgatgcag aacaaattaa ccttaatttc ttaacgcttt ttaaccaact tatagaaatt
    30781 agtggtattt tagttccaat atttcataac gacttactcg catcggagaa atattggggt
    30841 gtttttaaat ttattaataa actagcaaca agtagggatg agcttaaaat ttaagtcagg
    30901 attaatatta tttggtatta tattaatttt tacatttcaa actaatgcgc aggattacaa
    30961 attttcgcaa ttctataatt cgccattaaa cttaaatcct gcatttacag gtaagataaa
    31021 ttctttatat cgttttgtgg caaattatcg cgtgcaatat ttaccattgc aatcgccatc
    31081 gccatataac acacttagtg catcagctga ttttggtgtt ttgcgcgaca aactaaaagg
    31141 agatatattc ggtgttggtg ttgtgtttac caacgataga caaacagcaa ttcgtacaaa
    31201 tacgatgatg ttttcattgg cttatcacaa aagtttaggt aaacaaaaaa atcattatct
    31261 aagtgtaggc gcacaagtgg gttttttaca acgttctgtt gatttaggaa atttggcgtt
    31321 tgcttctcaa tttacaggaa caggctttga tttgtcttta aacaatcaag aaaattttgc
    31381 aggtgaaaaa tattttaaac caaatttgaa tatcggttta ttttggtcat ctaactttac
    31441 taagtcgatt ggtgcgtacg ctggtgtttc tgtgtttaat attattaaac ctaaagactc
    31501 atttttcaaa tcagataatg aacgtgccat gcgctttaat acacacgctg gtttgtttat
    31561 cgatgtaaag aagattgtat taatttctcc aaatggtatg tatatgtacc aagctaaggc
    31621 tcaacaatgg attgctggtt cctctttctc ttttaatgtt tctggtaaaa gtgaacctta
    31681 caaaacagca gtttcattag gtgtgtggta tgatggcaat ggcgctgtta ttgcgtcaac
    31741 aggtgtttct ttcttaggtt tgcaaattgg ctttagttat gatgctactg ttaaaaaaga
    31801 tctgaataaa gcagttaaat ctgtaggtgc tttagaagct tccattatct atactggcag
    31861 accattagac aaaaagaaaa gctattctcc attgctttgt cctaaattct aaacttctat
    31921 aatcaatata atttagctac tcatctgagt agcttttttt gtttcacgtg aaacaaaaaa
    31981 ttaaattcaa atttcttttg aattggtttc acgtgaaaca tactacaatg caaacaaaag
    32041 atgagcatat ttatgttgtt ataattaagc tctctaaaaa atagtgataa gtaaaacccc
    32101 aatacatttc tttaaatacg ctaaacatat tgaggaatat aggcttgctt atacaccata
    32161 atgcacactg cttttatcaa catgtgtatt gttttataga ataacacttt attaagataa
    32221 aatgacctcg tttgtgcacc gtaatgcaca tcatatttat taacatgttt attaatttgt
    32281 agaataacta tacatcgtgt aaggtattct agtaagcata gcttgaaaga aaatgaaaca
    32341 aaataactgg gtttttactg tattttgtag tatgtagaaa tataaatact attcaaagca
    32401 acaacgcgat gtatacatac gacactcagt aactaatttt taatccaact aatatcttat
    32461 aaatatcaac tttcacctac atttgtacta tgtttcaatc gtatgatgta attgtggtcg
    32521 gtgcaggaca cgccggctgt gaggcggcag tagcggctgc caaaatggga agtaaggtct
    32581 tactggccac tatgaatatg caaaccattg cgcagatgtc ttgcaatcca gcaatgggcg
    32641 gagttgccaa aggacaaata gtgcgcgaaa ttgatgccat gggcggttat tctggtattg
    32701 tttctgataa aactatgatt caatttcgca tgcttaatca atcaaaagga cctgccatgt
    32761 ggagcccaag agtgcaaagc gatagaatgc aatttcattt agtttggcgt gaaatgttgg
    32821 aaaacacagc caacatagat ttttggcaag atatggtgag caatcttttg gtggaaaatg
    32881 gtagcgtgtg tggtatcaga acaggaatgg gcatggaaat tagatcaaaa gcagtggtgc
    32941 ttaccaatgg aacatttctc aacggtatta ttcatattgg cgaaaaacaa tttggcggag
    33001 gcagagcagg tgagaaggca tcgaaaggaa ttaccgaaca attggtgagc ctcggatttg
    33061 aaagtggtag aatgaaaaca ggcacgcctc cacgtattga cggcagaagc ttagattatg
    33121 ccaaaatgga aattcaaaat ggagacgaca atatcgttgg tttttcctat aaaaacatag
    33181 agaaaccgaa agagcaacgt tgctgctgga ttacttatac caacgataaa gtacacgaaa
    33241 tactcaaaac aggctttgag aaatcgccca tgtttcaagg tagaattcaa ggtcttggac
    33301 caagatattg tccttctatc gaagataaaa taaaccgttt tgccgataaa gacagacacc
    33361 aaatatttgt agaaccagaa ggctggaaca ccgttgagat ttacgttaat ggtttttcta
    33421 cttccttgcc agaagacgtg caatacaaag cattaacttc cattccagga ttcgaaaagg
    33481 tgaagatgtt taggcctggt tatgccatag aatacgatta ctttccacct acgcaattag
    33541 ccttgtcttt agaaaccaga ttaatcaaaa atcttttctt tgccggtcaa attaacggca
    33601 ctacaggcta tgaagaggca gcagcacaag gcatgatggc tggcattaat gcacatcgtg
    33661 ccatacatga tgaagcgcca gtagtcctta agcgcagcga agcttatatt ggtgttttaa
    33721 tcgatgactt agtgaataaa ggcacagaag aaccttatcg catgttcact tccagagccg
    33781 aattccgtat attattacgc caagataatg ccgacttacg cttaaccgag ctatcgaacc
    33841 aaataggatt tgcggagcaa gaacgaatgg atcgagtaca attaaaaaaa caagccagcg
    33901 aagaaattat ccaatttata aaagaatata gcgtagaacc agcagaagtg gacacctttt
    33961 tagaatcaag aggtacttct gctcttattc ataaaatgaa attagaaaag attgtatcta
    34021 gaccacaaat tggaatatta gatttgattg caaacgttcc atccataaaa aagcatttgg
    34081 ataaatatga aatagaattt cttgaacaag cagaaattgc tataaaatat gatggctata
    34141 ttgctaaaga atatgatcag gtaaaaaaga tggacaaaat cgaaaacgta atacttaaac
    34201 ccgatttcga ctatatgagc ttgccttcat tgagcatgga agctcgtcaa aaattgtcta
    34261 aaatgaaacc acaaacgctc ggtcaagcga gcagaatttc tggcgtttca cctgctgata
    34321 tttccgtttt aatggtatat ttaggtcgat aatcattcta tcaattgaaa taatttatca
    34381 atgcatcagc atcattataa agtaaaaagc aaattatatt attacattat tgtattattt
    34441 caatgcctga ttttgtcttg cgctcagata acagcgccta ctggtggcga gcgcgacaca
    34501 aaagcacctg aaatagtatc aatttctcca gcaaataaaa gtaccaactt taataataaa
    34561 agcatacaaa ttgtatttaa cgagtggatt caagcattga ataatccaaa atcacaagtg
    34621 ataatttcac ctaatatcga accatttcca gtaatcgaca ttgctagaaa tgatatgaaa
    34681 attaaaatta aagacacact tcaatccaat acgacttata gcattttctt tggagacaat
    34741 ataaaagata ataacgaagg caatccattg ccaaatttta cttatatatt ttctacagga
    34801 aattttatag attctctgtc gctcaaagga aatataaata caaagctaga taagtttcca
    34861 gataatacct ttttgctatt gtataaagaa atggaggatt ctgtttttac taaaaagcga
    34921 cctttctaca tcactaaaat taaatcagat ggttattttt cgctagaaaa tatcaaagaa
    34981 ggacagtata aaatttttgc tttaagtgat aaaaacaaca actattatta cgatttgcca
    35041 acagaagaaa ttgcattttc tgatagtatg accacgataa atgcaaatgt tgatagtctc
    35101 tctttggaat tatttttagc agaagaagat aaattgagaa ttatcgaatt cgacagaacg
    35161 ctcaaaaatg gtatttgtac cgtattgttc aacaaagaat tgtcgccaaa taaagacgat
    35221 attacggcta cagttgttga tggtagcaag atagatgcta ttgcatttca agataaaaca
    35281 gcaaaaaaac tacaaatcta ttttcctaaa ttagaaaaag acacgaataa tttaattgta
    35341 gttcttaaaa ataatgccat actcattgat actttacgtg tacaatctta tcaaataaat
    35401 gcgaaacaaa cgattaactt ctttacagac tcgacagtag tgccactcat cgaaacacaa
    35461 gcattgcaat tgcatgcagc ttattatagt ttatcaaata tagatacgag taaaatacaa
    35521 attcgcgatt catcaaatac aagtattcca ttttctatta ctcgcaaaga tgatttacaa
    35581 acatatatta taagtgcctt atggaaacca ggcatgcgat acaaattgag cttgcaagat
    35641 tccgtaattg ctgatttagt tggaaatttc aataaaacac aagaattttc gttttcgcct
    35701 atgtctgcta aaaaagcagg taatctgttg attacgtatg agttaccaca aaataatgta
    35761 aattatatcg tttttctgaa gaatagtgca ggcaaagtac ttgataagca aattatacgc
    35821 gattctcagc gtgttcaaat taactatggt ttaacattgg caggtacata ttttatagaa
    35881 gtcgttgaag atagtaacaa caatggcatt tggaattctg gaagttttat caataaaaca
    35941 ttaccagaaa aaatatataa agaggctaaa ccaattatta taaaagagaa ctgggacgca
    36001 gaagaaacaa taaaagttga tttttctgca tcaaaaagtg tcggcataca aaatattaca
    36061 tcacctcaaa atacaccagc atttaattca cctaataaaa caaataaagg tgctacgaat
    36121 actaagcaat aggtatattt aattataata aagttccacg accaaccatc aagattactt
    36181 atatcagcaa ataagcatgt cacactgatt cacaagaaaa attactttat gcacaatatc
    36241 accatttttg gtgatgcggt gtgtgaattt tatacttaat tttacgataa cataaaaacc
    36301 ctcttatgtt aaaagacata aaacctaaaa ttcattcttt attgatttga taaataaaaa
    36361 aa
//
LOCUS       DAOQQV010000015        35939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_336_length_35939_cov_7.146806, whole genome shotgun sequence.
ACCESSION   DAOQQV010000015 DAOQQV010000000
VERSION     DAOQQV010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 35939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35939
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_336_length_35939_cov_7.146806"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /gene="thiH"
                     /locus_tag="PLL59_03095"
     CDS             <1..380
                     /gene="thiH"
                     /locus_tag="PLL59_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614531.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and iscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HMY22821.1"
                     /translation="LQRTYWQTKYSVSFPRIRPHEGKFIANTHLTEKELLQLICAYRI
                     CFPDVELSLSSRERAGFRDIIFPLGITSMSAGSKTEPGGYSKSEALKQFEVNDERSAQ
                     EVANIIRAKQYEAVWKDWDAILV"
     gene            complement(398..1693)
                     /gene="hemL"
                     /locus_tag="PLL59_03100"
     CDS             complement(398..1693)
                     /gene="hemL"
                     /locus_tag="PLL59_03100"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HMY22822.1"
                     /translation="MKKDISNQLFEKAKTLMPGGVNSPVRAFRSVGGTPVFMKKGKGA
                     YITDEDGNEFIDYCCSWGPLILGHANENTEQAIIETLKNGTSFGAPTALENELAELII
                     SNHSCIEKIRFVSSGTEAVMSALRLARGVTKKNKIIKFEGCYHGHVDSLLVKAGSGLV
                     TFGESVSAGVPDSFANETIVLPLNDEDAVKDCLEKLHQDIAAIIIEPIPANNGLLLQS
                     HKFLLHLRELCTQYNVLLIFDEVISGFRVGFQGAAGLYEIKPDILTFGKIIGGGMPVG
                     AYGASHVIMDHISPLGSVYQAGTLSGNPVAMSAGLAQLKACLQPHFYQDLQHKTDVLV
                     DGVKKHIQAKDYEITIYNIGSIFWFAFSDMTHLTRADQINAGKMSLFAKVYHYLLEHG
                     IYFGPSGYEVGFVSAAHSTADIQKTIQVICDAFDKVMAE"
     gene            complement(1735..5400)
                     /locus_tag="PLL59_03105"
     CDS             complement(1735..5400)
                     /locus_tag="PLL59_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMY22823.1"
                     /translation="MQFLYRHKISIITIIGCVLLSVGLLVDTLQSISPAINRAKSIIE
                     TRLHKTEKKANAILHNQQVLAATNEYAAVRNAIQHLDNDKDILIYIYKDGILNYWSDN
                     SFIPDNIYKLSQHDIYFLKEGNGFYEVLNNKISDGVQLYALIPIYHQYATDNKFLING
                     FVWKHPFLKRFVISSKETKTSSRINSINGTYLFSIRIADDAQKVYNPYIIVVELIGLL
                     LLFWAMYQVIKNAFVKRKNKLAFAFIVLVIVATDVMFVELQLFSLTKTSLLFSSNLYA
                     SAYLGDSLGALLVRVFLLAWSLSFIRFIQTDFNKQYYKVWLLILPIAVYFLLVFIIQS
                     VIQNSVISFNFYLANTLDRYTFISLLVFGFGFSSLLFLMKWIFEHNIDKHFFKYVFVF
                     GVFGLLIGFKLTLFTDVLYACFLLLWLLTFVLFFYYEKSFFQQNKKYKFLSNLILLSF
                     IAFLTSVIIIVNTNQKDIEKRKYRMEELVSERDIGEEYTLTESENNILQDNFVKSYFK
                     NPYLSTVNIENRITSKYFKPLLRRYTINTYAFDKDGMPLIGVTQKEFYKLNNTRHSRK
                     AKSISSQYYYLSIKENGEKYLGYFPIQDDSLNIGYLFVEFIPKIFSSYSAYPELLLKH
                     KNYFDEEFDNFSYAIYDNKYLVKQKGDYEYQVTFNFPVKENKEFSVYKNDDYTHMIYF
                     SKEKQVVLSEKNRPALSTLSVFSYMVIFFLAFFLCMDLFGFSDRFWDETSIRNFFKGN
                     TLQKQIQNSMLALVLFSLAIIGIVTLVYFQYQYNIYHNSKLLKKVNAVMKNTAQYYVE
                     EYPVYGDNTFDKIIQQKIKVLSSIHALDINVYNVKGDLIQSSQPEIFKRNLVSSKMDA
                     DAYYNLIVKEKSKYVHDEKIGKLSYLSAYQPFRSNGKVLGYFNFPYYGKQKSFQEDIS
                     YFLVALVNVYVVFLIAAALLAVFLSRSITNSLTLISDNIKDVQFGKFNKKIIWKNEDE
                     IGLLVHQYNLMLDELEKSADLLAKSEREGAWREMAKQVAHEIKNPLTPMKLSIQHLQR
                     ALKDNSPDITELTSKISHRLIEQIDNLSGIATAFSDFAKMPQGVFKPIDIVPILESTV
                     ELFREEENINISLDYPSTACVVLADKDQLMRVFTNIIKNAIQAIPENKIGLIDIHLLV
                     ASDNYLITVKDNGVGIANEKREHIFEPNFTTKSSGTGLGLAISRNIIERMNGKIWFES
                     IVDEYSIFYIQIPKTIN"
     gene            complement(5407..6186)
                     /gene="mazG"
                     /locus_tag="PLL59_03110"
     CDS             complement(5407..6186)
                     /gene="mazG"
                     /locus_tag="PLL59_03110"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HMY22824.1"
                     /translation="MDNRLVAFARLLNIMDDLREKCPWDKKQTVQTLRKLTIEETYEL
                     SDAIIKEDWKSIKEELGDLFLHLVFYAKIGTEQEQFSVADVLNSVSEKLIYRHPHIYG
                     DVTVEDEEEVKRNWEKLKLKEGKKSVLEGVPNSLPAINKSQRIQDKVRAVGFDWERKE
                     QVMDKVMEEIAELKEAVEQGNKQEIELEFGDVLFALVNYSRFIDVDAEMALEKTNSKF
                     IQRFQWMENYTKEKQLDMHAMNLEQLDEIWILAKKELLNKA"
     gene            6252..6662
                     /locus_tag="PLL59_03115"
     CDS             6252..6662
                     /locus_tag="PLL59_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22825.1"
                     /translation="MKKLIADYKNIHPIKQGVFFLLLISLFAVSCLIFNIKNMMAWNM
                     LILAFMLFCVFNPVISTFQQKLLQYFLFSIPVFIGLLFYVYFVGNFISNINYNQSHEL
                     HFIALLIIIFYALAIMFSLIFRGVLHFLENVDNQ"
     gene            6736..8001
                     /locus_tag="PLL59_03120"
     CDS             6736..8001
                     /locus_tag="PLL59_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMY22826.1"
                     /translation="MTRAAIRNIILLAFISLAGIIATQLFWIKKAIDLKEQQFNHRAY
                     VALKSVADRLVLDTKYRVQLDGINKKASNYYTLEFSSPVNVTMLENYLVIESKNQNLN
                     ADFEYGIFECTTDSFYYGRYVNIKKTGEIERLKVKAKPSENYYIGVLFPKKKTYLQDD
                     LNLLIFFSAVLLVVIGFFTYTILVILKQKKLSEIKTDFVNNMTHEFKTPISTISLSCD
                     VLMRDDITKNPERLKHYATIISEENKRLKSQVESVLQVSQIESHKIALNYSEVNMHAL
                     IENIAKNIKPRLDKQNGHIQLHLYDQKHIVLGDEVHLTNILYNLLDNAIKYCDKIPEI
                     SITTVNKHKGIEIAVKDNGKGIETSSHSMIFEKFFRVPSGNIHDVKGFGLGLFYVKNM
                     IKLHKGKIALHSVPTVGTTFTIWFHTIEK"
     gene            8007..8696
                     /locus_tag="PLL59_03125"
     CDS             8007..8696
                     /locus_tag="PLL59_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMY22827.1"
                     /translation="MDKVKILLVEDDPNLGFVVKDNLSDKGYEVTHCINGLEAERVIY
                     KEKFDIYLLDVMIPKKDGFTLAQELREKKDDTPIIFLTAREMQEDKLKGFSVGGDDYI
                     TKPFNFDELTMRIEAILKRTKSTTPEQKEFNIGKYILKVDELKLINGDEVSSLTKKEA
                     ALLEFLVKHVNQPVKREDMLVKIWKDDSYFAGRSMDVYITKLRKYLKDDSNIEIQNVH
                     GVGFKLIDNKL"
     gene            complement(8760..10778)
                     /locus_tag="PLL59_03130"
     CDS             complement(8760..10778)
                     /locus_tag="PLL59_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22828.1"
                     /translation="MKNKILFLLILSVISGNIKAQDTTNIDSILSVKLDEVAIISKPL
                     INYTKQNKPLSSIDEYLDKSMNVSLVRRGSYAWEPMLNNMSSERLQLTIDGMHIFGAC
                     TDKMDPITSYVDVSNLSDVKVSSGQEGASYGPTIGGAIDLIKKKHDYCHIGWLGSVNF
                     GYESNSNLFTAATRVKYSNDKFFVDADFIFRDADNYKAGKRTLIPFSQFQKYNTSLSI
                     GVKINNKNSLTGSFTFDNAVNIGYPALPMDVALARAYIASVQHSASYINSFIDEWTTR
                     VYFNTITHIMDDTKRPIVPIHMDMPGWSTTSGLYSKINGAHKRQKMALTLNAYWNKSK
                     AEMTMYPNDTSRKSMFMYTWPDVHTIYAGVNLANNFSWKPMHTIKYGASIGFHRNFVA
                     SEFGLNSMRIFYPTMSAGKNRIIVGLQTAYTLAKNNWEYSIAMAYGERAPSISEAYGF
                     YLFNSFDAYDYIGNPYLKNEKSIELHMALAYKMKKIKWNFGLNYFHILDYIIGIPNTN
                     YIAMTLNANGVKEYQALESVNIVSTECGMEYRPHPSVKTRVALSYNFGRDFNKQNMPL
                     VRPFSFKAGLTYNIKGLEMEMNVEGASKQYAHSAFYGEKAKPAYAIVNFAAGYSLPIK
                     AHQLHFRVGIENMADTYYSTYADWNNIPRKGRNFYLNISYTFDNRKME"
     gene            complement(10831..11670)
                     /locus_tag="PLL59_03135"
     CDS             complement(10831..11670)
                     /locus_tag="PLL59_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HMY22829.1"
                     /translation="MKKIIFIAIAMCSIILISCKKETAQQPDELAGLTLLQKMVNDTT
                     EIEVYTRSGTLTVGYNDIFFRVKNKLNDEFVSNAVLDWMPIMHMTMMSHAAPKSPITK
                     VAGKEFLYKGFIVFQMPGNDMEKWTLSIDVQTPSITTAVLDTFAVANSTKKRVASFTG
                     SDSKKYILALVSPENPAVATNDLTLGLFVKETSMSYPAVTDATILFDPRMPDMGNHTS
                     PNNVQPIINTTDNLYHGKVSLSMTGYWKLNFIVKNASDVVLKGEEISPSVESSSLYLE
                     LEF"
     gene            complement(11762..12139)
                     /locus_tag="PLL59_03140"
     CDS             complement(11762..12139)
                     /locus_tag="PLL59_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22830.1"
                     /translation="MKFIIVIIVSFTTLLRPILPVMDYAINYDYIQKVLCINKNNPEK
                     NCHGKCHLNENLNKVFDDKDAKSTTSKNSTEELISLICQKHTNKIFIIKNDISTDIKL
                     GNFIDSYTYTFSSVIFHPPCKIV"
     gene            complement(12241..13170)
                     /locus_tag="PLL59_03145"
     CDS             complement(12241..13170)
                     /locus_tag="PLL59_03145"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HMY22831.1"
                     /translation="MSQLSSKHVIGIEHLTVDDIQLILKTAVQFKEIINRPIKKVPTL
                     RDITIANLFFENSTRSCISFELAQKRLSADIINFSSSSSSVKKGETLLDTVNNILSMK
                     VDMIVMRHASPGAAHYLSERIDANIVNAGDGTHEHPTQALLDAYSMQEKIGDLKNKKI
                     AILGDIMHSRVALSNIFCLKKLGAEVTLCGPATLIPKHIERLGVNVSHDLDETLQWCD
                     VANVLRIQLERQNTKYFPSLREYALYFGVNKQRLDKLSKEIIIMHPGPINRGVELSSD
                     VADSEHSIILEQVENGVAVRMAVMYLLANKQQF"
     gene            complement(13187..13720)
                     /gene="pyrR"
                     /locus_tag="PLL59_03150"
     CDS             complement(13187..13720)
                     /gene="pyrR"
                     /locus_tag="PLL59_03150"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HMY22832.1"
                     /translation="MSDTVLLNGDQIQLTIERLSREVIESIHFTNTVIIGVQPRGVLL
                     AEKIHQKIISLTGVQIPFGKIDTTFHRDDFRRGKSLNAKVTEIDFHIEGKEVLLIDDV
                     LYTGRTIRAAMDALLSFGRPTKIELLVLIDRRFNRELPIQPDFVGSVVDTIESVDVEV
                     HWAGEEQINNKVIFVKK"
     gene            13862..14251
                     /locus_tag="PLL59_03155"
     CDS             13862..14251
                     /locus_tag="PLL59_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HMY22833.1"
                     /translation="MHKTFGIIAALYGGLAVVLGAFGAHALKNKLDAYQLDIFNKGVQ
                     YQFYHALALIAVVLISDKLNTKTLTIAGWNFSLGVLFFSGSLYLLAMRHLLGMEGFAS
                     ILGPITPLGGLCFIIGWIFLLISFVKM"
     gene            14344..16836
                     /locus_tag="PLL59_03160"
     CDS             14344..16836
                     /locus_tag="PLL59_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY22834.1"
                     /translation="MSTTFGQPPQQFGTAAGSISGKVVDAAKSSPLAYSSVTLQSLKD
                     SAIILGSLVDEKGFFEITKIPFGAYQLTVSYIGYSDYKKQPIVVAPPDKMSVNLGTIK
                     VSEDSKLLDEVQITAEKSYMQVNAEKKVFNMDKNILSTGGSAIDALKQVSSVEVDQDG
                     NLSMRGSGNLRVFINGKPSGITVTNTKAILDAIPASQIESIEVINNPSAKFDAEGEVG
                     IINIVLKKNLKNGLNGNITIGYGTKYDANTGATISYRKNNISFTTSYNFRFTESYFKG
                     TSQRFNFFPNQTPFYLNNSDYGKYHTFANTINSSLDWSIKEKNTLSFSVLLSTSNSKT
                     NARTSTHFLDTLVEYVNAYNRYNKDKQFNWNGEINTSYRRTFKDNSNDLVLAANYAYS
                     DRKSSPIYTQKTLDVNDEELENIDLLQRNKHTNITHTAFAQADYTQPFKKSKSKLELG
                     YKYSFRSIATALNGDSLLSSTQQYVYDSSISNQYNYREQIHAAYMIFGGAVKKIFNYK
                     AGIRLENTALNIHQQRDSVIHKQNYFNYFPSASFSFNLPKSHALSVSYSKRINRPSAE
                     QLNPFGNYSDPYNILTGNPNMKPSYTHAVELTHVKNIDIKPSKRDSTFNRSVFFSTTI
                     YFRHAYNVFTRFRTVDSSGHSIVNFDNLSSGQNIGLEFTNRTSLFKWWNFVLTGNFFY
                     NSVKGNVPNGEVDATTNSFQYNLRLMNTFNITPKASLQFMVMYRSKIKFLQGEITPMV
                     FANIGFKYDFLKNNRASIAINISDMFHTQYFGVRTSGNTFTATTSRYWESTVGNIVFT
                     YRFGKSDSKNAISKQKKSNFEDSGNGVEGGGG"
     gene            complement(16839..18011)
                     /locus_tag="PLL59_03165"
     CDS             complement(16839..18011)
                     /locus_tag="PLL59_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY22835.1"
                     /translation="MNKILQVAKLNKAKSYLSKAWIIGLFFCFFLVSNNTFARKKTDL
                     VLEFAKEQIAQKFYQPAISTINSYLESHKNDTVALYWKAFCFYKLKNFQAANENYTLL
                     LKLHPKCYAAYVDMGNMLVLDKKFDDALPYYNKAVKMNDSDINLFNSRGMCYYYADKF
                     ELAIKDFRRVIKLDPNNYIAYNNIGSATYNNQNIAEASVVDLKLAEAQFNKSLELKPD
                     FELANRNRGIVRYHLDKIDEAYNDLLYATQLDPNDESAQYYLGKVLVKQKNYTIAIQF
                     FDNAINLTNYKSEYYLDRGMCKLELENFKSARSDIYKSMQLTNERAYAYYQIARTYAA
                     EGEKNNTFTYLREAKKAGLFNDNRYFGYIAKDKYFVGWAKDEEFKDFMYTLKFGKN"
     gene            complement(17977..20040)
                     /locus_tag="PLL59_03170"
     CDS             complement(17977..20040)
                     /locus_tag="PLL59_03170"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HMY22836.1"
                     /translation="MKFKNVHLSYTTAQNEKFEINTSDFSIIENIHLKIEFILEEIGD
                     DKRYVVYFNAKEKIELQYFYINADFDYSASNSIFCNGFQSWTETKLFNKQEKLQSQRR
                     GVKIIGEAYGDGTFFNYKNKVGVFHSWTYTYIKLPKRKIFFLGSLSDAKSYTIFEHDA
                     SQNNMQIISDCNGMQLAANTSLNIIDVFASNTYEWQCFQNYFEAQHIKPIHAKPAIGW
                     TSWYYYYTKISEKVIVENIKAFVDNKIPISIFQIDDGWQQAVGDWLNINHKFPNGLLP
                     IVEDLHKHKMKAGLWLAPFACEHNSFIVKEKPHWILKDDKNKFLKIGFNPLWSFWFYA
                     LDVYNEEVRTYLRTVFKTILHEWKFDLVKLDFLYGTALIARNGKSRGEIMHDAMAFLC
                     ACVGDKMILGCGVPLSAANGTTEYCRIGPDIHLSWDFSLLKWIRARERPSVLNAIHNT
                     ISRRHLSGKWFWNDPDVFLLRKNKNSLSFEEKYSLLLANLLFGHLLFTSDNIAEYDQE
                     QLFWYKSIFPLMAIKDAQVHEQDNFYTINVHVKDRYYLILINASDSSKMYKLPSNLYF
                     DNVKEDLLKGDKRIEIPKHTSRCLLTVGYTPFAIAGSKGHFFSGTEIENIYITGNNIE
                     LEWTSGLLNEVTLYIKVPSNYEVITINNSGNFKRIEKQDFSIIVVEKHKLSLGEQNIT
                     SSQAE"
     gene            complement(20254..20326)
                     /locus_tag="PLL59_03175"
     tRNA            complement(20254..20326)
                     /locus_tag="PLL59_03175"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20291..20293),aa:Arg,seq:tct)
     gene            20427..21107
                     /gene="ung"
                     /locus_tag="PLL59_03180"
     CDS             20427..21107
                     /gene="ung"
                     /locus_tag="PLL59_03180"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HMY22837.1"
                     /translation="MSEVNIQIEDSWKDALKREFELPYFTELRQFLHTEKQEGKIIYP
                     PGTLIFNAFNTTPLAQVKVVIIGQDPYHGAGQAHGLCFSVQHGIKPPPSLVNIYKELY
                     ADVGFNIPTHGCLQKWAEQGVFLLNAILTVEANKPASHQKKGWENFTNAVIQKLSEQR
                     NGLVFLLWGNYAQQKATLIDEKKHTILKAAHPSPFSAYNGFLGCKHFSKTNEILQSQG
                     IAPIDWQV"
     gene            21204..22121
                     /locus_tag="PLL59_03185"
     CDS             21204..22121
                     /locus_tag="PLL59_03185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY22838.1"
                     /translation="MALKNIRYNFTPVEHCIMCGERVATHKVLGKRMNQSQGKNPKNK
                     VGITTSVLQCSKCHLIYSNPLPVPFDIQDHYGVPPESYWKESYFVIDEKYCQKEMDHL
                     KQLMDIKPGMKALDVGAGLGKGMLAMQRIGLDAYGFEPSEQFYERAIHKMGIAPEKLK
                     IGMIETVDYPENEFDFIMFSAVLEHIYDPAAAIMKAMKWLKPNGIIFIDVPSAHWLTS
                     KILNFYYRITFSDYVCNISPMHDPFHLYEFSIKSFEAHAQKNNYEIISYSYEVCSTYL
                     PKSLDFIIKPIMSKTDTGMQLDVWLRKKK"
     gene            22139..22612
                     /gene="coaD"
                     /locus_tag="PLL59_03190"
     CDS             22139..22612
                     /gene="coaD"
                     /locus_tag="PLL59_03190"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HMY22839.1"
                     /translation="MNKIAIFPGSFDPITIGHVDIVNRAIPLFDEIIIAIGINTQKKY
                     LFSLEQRIAWINKVFANAPNVKVESYTGLTVNYCREKKAKYIIRGIRSSADFEYEKTI
                     AQLNKMMMPELDTFLILSSPELSAISSTIVREIIIGKGDTSKFLPKEVDISNVTI"
     gene            22789..23289
                     /gene="rpsP"
                     /locus_tag="PLL59_03195"
     CDS             22789..23289
                     /gene="rpsP"
                     /locus_tag="PLL59_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HMY22840.1"
                     /translation="MATRLRLQRHGAKGAPFYKIVAADSRARRDGKYIEQVGTYNPTT
                     IPATIILDVDKAVKWLQNGAEPTTTVKAILSYKGALYKKHLLRGVKLGVVKAEDVETK
                     FNEWAAQKDGKVLDHKAKADAKKIEKNLAVVNTPAKVKEVPAEETPATENTEEAGTEA
                     NTETAE"
     gene            23378..23875
                     /gene="rimM"
                     /locus_tag="PLL59_03200"
     CDS             23378..23875
                     /gene="rimM"
                     /locus_tag="PLL59_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HMY22841.1"
                     /translation="MELIKIGKIAKTHGFKGHIKVYIDEFYMNDFEDMQAIFINQLPY
                     FISKKDINSNSQAILLLEDIDTKEKAQKLQGKEIFAKDDDLTEILDGNEYDELINFIF
                     MDKSSGKNGKIIEIIEMPFQFLAKVQFEDRETLIPLNDDFILSINRKKHIVEMCLPEG
                     LLDIN"
     gene            23893..24573
                     /gene="trmD"
                     /locus_tag="PLL59_03205"
     CDS             23893..24573
                     /gene="trmD"
                     /locus_tag="PLL59_03205"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HMY22842.1"
                     /translation="MRIDILSAVPDLMQSFFAHSILQRAKERNLLEIHLHNIHDYSKK
                     KYKQIDDYQFGGGAGMVMSIEPILTCIEKLQAERKYDDIIFMTPDGELLEQKMVNSLS
                     LKQNIIILAGHYKGIDERLREHVITKEISIGSYVLSGGELPAAVLVDAIGRLIPGVLN
                     DETSALTDSFQDNLLAPPVYTRPSDYKGWKVPEILLSGNDKLIAEWRHQQALERTQQR
                     RPDLLEND"
     gene            24631..24987
                     /gene="rplS"
                     /locus_tag="PLL59_03210"
     CDS             24631..24987
                     /gene="rplS"
                     /locus_tag="PLL59_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HMY22843.1"
                     /translation="MEKVKELTKKLTETKQIPDFKAGDNINVHYKIIEGSKERIQIFR
                     GDVIQRKGTGATQTFTVRKISNGVGVERIFPLLSPNIDKIEVNKIGKVRRAKIFYIRE
                     LKGKASRIKELVKTTK"
     gene            complement(25279..26832)
                     /locus_tag="PLL59_03215"
     CDS             complement(25279..26832)
                     /locus_tag="PLL59_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMY22844.1"
                     /translation="MKIHKIISLVALLVAGIQLSDAQQLPVFNNYFLNPFFYNPARAG
                     TDLDGGRINLGFQKQHDRQPDGPITTYGSWDGRIKSGNMALGVTIYHDRTTPQWQNTG
                     GSVAYAYHLPFKKDKTHFLSFGLQAGVNSIGLHNFTPEDANDPLTANGRSVNFDLSLG
                     INYHWKGLNVGFSVPQVLGNKVRFKTTDPQFSHYTRIERQYMFMVSYDAKLGKKKEWT
                     LSPFAQINFIKKSLPVYLDLTLMAQYKKLVWFAVGYKNGGGMKPTGNSDKGWIYSNAA
                     GIHATAGVGIKELVNISYTFKAPVGKKSFNTSFANLGSTHEILLSFRLKRKTDQDEFE
                     KNKKRTDDEIAKVNQKTDDNAKKIDEVDAKVDPLGNALTNVEKTANEAKSASEKNTSD
                     IEKVAQRLDNIVYKKFGSVYFEVDKAELTDEAKASIDAFKGKLAEMKGNYFIYLAGNA
                     SSEGGTTYNQVLSAKRCDAVKRYLEGVGISQRVLLLPYGENSWVTEKQNKEEDRAMNR
                     RVDIYLSGE"
     gene            complement(26875..32526)
                     /locus_tag="PLL59_03220"
     CDS             complement(26875..32526)
                     /locus_tag="PLL59_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY22845.1"
                     /translation="MKKRFLTKTPNWKNYCSLLLLLFMFGLQKPLSAQQFQTITVTWT
                     GGSDDDCSDQGLGGCSSFTGSPDPRWLLEGKLNTDATYSPVYFRGLDNVMAGSHTWGT
                     PQQVFSTTVCGATFVNIRASSWEEDNIACGSNTTYDPGCALPDDDYSGVQLFNIPVAN
                     GAWSRTMANGYTIFGTMSVVNAAAGLPAPTVAAVDVEVCYNATASFSVTSNASVPGNM
                     FAWYDDAALMSPIAYGSQYTTPPLTASTTYYVAEMDQAGGCIGAATAINVTVRPQLPT
                     PDVDVSLACFDGEAQIRINDLGGGELYDVSLNPDFVAITSTVPAGTYFFNLSSTQPTV
                     YYVRVNDGVCTGPVIPAIVDVVDKPEIAIDDAEACEGDPFVDFFVHTTLTGWDPANDA
                     NLVIYDGGFNLFGVLPFDGLDASFSLGLSPAIAPGTYLFYGQLSNSYFDDLDGNPVYC
                     DGDLVPFYLTINDTPDAPTAAVDTVCVGQSTSLVATGEDGATLTWYDDAGLTHAVQVG
                     AEYNTPALSSTTTYYVTAKYGECESEATAVEVFVNPPAEMPYAAQDYTICLGQTVPPG
                     EGLQAACENAGGVVPVITTVSIPGVAGGGGLTIGPDEGYSGSMTFDASAIPAGATIDN
                     VSITLNMRHTWAADVYLRLTSPAATSVDVTNPVWLSTLSSNFGTGNGTVSATYTFDDA
                     GPNTLTPGLGSTYDFPAGTYFPYSALSAFNGEDPSGSWTLDVEDLFGIDAGVIESATL
                     NVSYTSGSSATANIGGTLPSVGFPVPIGPSPNPQSITFDASALPAGAVVTKVTATVEM
                     AHTWAGDVFLSLTSPSAATAEIVNDNGLGSDNYGTAGTDVVTYTFDDAAAATVNPISG
                     SDMDIPAGSYRPNETFTVFNTTPAQGIWTLNIDDLFGGDGGEIASASLEITYSVDNLV
                     AGNVLTWWDSPTGGTQVGTGSPFLPNQYDTLAPGAYTFYAQCDSVSTCSNSRTPVVLN
                     VLPQATAPIVARFDAVCAGSSAILTVTNPIGQVEWYADAALSTLLHIGATYTTQPLNA
                     TTTLYVVNNNGTCLSPTTTVVVTVNERPEMPEAEEGFYVTCWDDATVLFASNSNGDEI
                     HWYLDKGGLDEVTGFFGFDGNGEFTTPEIASWTRFYFDAVDPVTGCHSQMNFVDVYAT
                     PEFPAPRVDDVTVCESDETITLTAHVTFPHDLIDDFFDVFTFAQAIVQFTDNTGTVSG
                     PLTTLDLATAALDPWNGVFESAVSITIPKVGTGPFGEDYDYSVPGTYDIGATVNHVWF
                     NAFTGDPFFCLSDFGTASLVITETPEAPVAEDVTVCEGDDAVLTASCDGEIRWYSDAA
                     LTDMIHVGSVLNVTAPAATTSYYATCTKDNCESAAEEVVLTVTETPATPIINSNTPVC
                     GEGDLVLTCTTVAGTGVQYNWYGPDGSLLGTTTDTTFTITNVTPAQSGLYSVTASIGS
                     CVSEATYTTVVIREKPVAPVLPAGPLSVCERGTVTFCASTPEAGAVYNWSGPNGFIFN
                     GNCVTITDATAAMSGEYVVSVTIDGCTSDSSRVTLIVNAAPTVDSISSNAPLCEHQTL
                     QLTTHVSDGNPYAYQWSGPNGWSSTEQNPSIADVTEVDHQGFYTLVVTDTLTGCTSKV
                     YSELVEIYTFPDKVIADNDGPICEGGVIKLNATNVFGATYTWTGPNGWTATGKNPTLD
                     PASPSQTGTYTVTVTLPGGCADSATTDVIVWANPIAHAGDDTSIIQGTIFQLNGTSDN
                     GPLPILPGITFNWTPNELVNIDNIPNPYVDFTELPVPNPYVMVFTIWDKNGCTDKDTI
                     EITVIPSLDLIIPDIITPNGDGLNDSWFIQHIENLNNAQIPYTVQIYARGGALVYTTT
                     AYDNSNGFDGTYKGTTLPEGAYWFVIVTPNKTYKGALHIKR"
     gene            complement(32658..33476)
                     /locus_tag="PLL59_03225"
     CDS             complement(32658..33476)
                     /locus_tag="PLL59_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22846.1"
                     /translation="MKNFLHLALILFLSTTCKAQLTKEQYKLAAEVLYRKTSFLDQLK
                     IKQETFAELAYLKRTHQLDSFNLQTKSYIANLNDYYYKDFYLYSKLLNENAIITEDCI
                     QEAKLLPIRKLLFYCIYPQSVKLPNDIVEQLEEQATVDEFLGPYYALNNIYFLKKYNS
                     HNLSAQQKSKLEKTEQFFSNLLFNKYVKDKEEWNYHKFLSLKVLRMNKYQPAENINIE
                     PLAYFILKDISTPLALGETDLNDSKLMNSIGYYQTLEMEANALLWIFLLEVDKK"
     gene            complement(33535..34038)
                     /locus_tag="PLL59_03230"
     CDS             complement(33535..34038)
                     /locus_tag="PLL59_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMY22847.1"
                     /translation="MNDINFKSEVKLATTVLKPHAMRLTSNIYDAEDLIQETIVRALV
                     NEDKFQEGTNIKAWLFTIMKNIFINDYRKKSKRNTVIDTTANLYYINTSSTISNAGER
                     AFVMNDIRNALMKISNELRVPFLMHYKGYKYHEIAEQLNLPLGTIKSRIFFARKELKN
                     LLEDDYK"
     gene            34297..35853
                     /locus_tag="PLL59_03235"
     CDS             34297..35853
                     /locus_tag="PLL59_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY22848.1"
                     /translation="MKSLYFTEEHELFRQSVRQFVQKEILPYGDQWEKDEKIPRDLFL
                     KLGEQGFLGINHEEAYGGSKADIFYTCAYLEELAKCGYAGVCAAVSVHQYMATNHLAE
                     AGSHEIKEKFLRPSIEGKKVGAIAVTEPFGGSDVQGMRTTAQKDGEHYIINGSKTFIT
                     NGHFCDFVVVACKTDSNAGINGISLIIVERDTPGFTSSQLKKIGWHSSDTGELAFDNV
                     KVPVSNLIGKEGMGFFYIMESFQIERLVAGIIGIGGGDHCLEETLKYMNEREAFGRPI
                     KKFQVLRHEIVQLYTELEAGKQMTYNTCWLMQNGEVPVKEASMVKLYMTELGNKIVDK
                     CLQMFGGYGYMEDFPIARAYRDARVGTIVGGTTQIMREILSKIIIDDIRYKKVYSNDS
                     SVAAASNSNSTNNEKSWGNPSTAKEIIASIPLRIKKEKAADYSTTFQFDIAGETGGQY
                     TLFVNKGDAKIEAGLNGTPECIITADAKVYEDIELGRMDPTMAFMGGQIKVTNIGAMM
                     QFAKYFHRIQ"
BASE COUNT        12008 a         6376 c         5918 g        11637 t
ORIGIN      
        1 atctacaacg cacatattgg caaacaaaat attccgtttc ctttccgcgc atacggccgc
       61 atgaaggcaa attcattgct aacacacatc tcacagagaa agaattatta caattaattt
      121 gtgcatatcg gatttgtttt ccagatgtcg agttatcttt gtcatcgcgc gagcgtgctg
      181 gatttagaga tattatattt cctttaggca taacatctat gagcgctggc agcaaaactg
      241 aacctggtgg ttattcgaaa tcggaagcac taaaacaatt tgaagttaat gatgaacgca
      301 gtgcacaaga agtggcaaac atcattcgtg ctaaacaata cgaagcagtg tggaaagact
      361 gggacgctat cttagtatag ttaatcttat tcaaaaatta ttctgccatt actttatcaa
      421 aagcatcaca aataacttgt atcgtttttt gaatatcagc cgtgctgtgc gctgcagaaa
      481 caaagccgac ttcataacct gatggtccga agtaaatgcc atgttctaat aaatagtgat
      541 atacctttgc aaacaagctc atttttccag cattgatttg gtcggcacgt gtaaggtgtg
      601 tcatatctga aaatgcaaac caaaatatag agccgatatt atatatcgta atttcgtaat
      661 cttttgcttg aatatgtttt tttacaccat cgactaaaac atctgtttta tgttgtaaat
      721 cttgataaaa atgtggttgt aaacaagctt ttagctgcgc caatcctgca ctcatagcaa
      781 ctggattgcc actcagcgtg cctgcttgat atacgctacc taaaggcgaa atatgatcca
      841 tgattacatg gcttgcacca tacgcaccaa caggcattcc gccaccaata atttttccaa
      901 atgtgagtat atctggtttt atttcataca aaccagcagc tccttggaag ccgacacgaa
      961 agcctgatat aacttcgtca aatatcaata aaacattgta ttgtgtacac aattcacgca
     1021 agtgtagtaa aaacttatgt gattgcaata gcaagccatt atttgctgga attggttcaa
     1081 taattattgc ggcaatgtct tgatgtaatt tttctaaaca atcttttact gcatcttcat
     1141 catttagtgg caatacaatt gtttcgttgg caaaactatc aggcacacca gcagacacag
     1201 attcgccaaa cgtcacaaga ccagagccag ccttcactaa taaggaatct acatgaccat
     1261 gatagcagcc ttcaaacttt ataattttat tttttttagt aacgccacga gccaatcgca
     1321 atgctgacat tactgcttct gtaccgctac tgacaaatct tattttttcg atacaagaat
     1381 gattgctaat aatcagctct gccaattcat tttctaatgc agttggcgcg ccaaacgatg
     1441 ttccattttt tagcgtttca ataatagctt gttctgtatt ttcgttggca tgtcctaaaa
     1501 taagcggtcc ccaactgcaa caataatcaa taaattcatt accatcttca tctgtgatgt
     1561 atgcgccttt tccttttttc ataaaaactg gtgtaccacc aacagaacga aaggcacgca
     1621 caggtgaatt aacaccacca ggcattaaag tctttgcttt ctcgaataat tgatttgaaa
     1681 tatctttttt catctgtata aatttaatgg cattgccatg ttacaaaggt atatttaatt
     1741 tatcgttttt ggtatttgaa tataaaaaat tgaatattca tcgacgattg attcaaacca
     1801 tatttttcca ttcatgcgtt caataatatt tctgctaatg gccaaaccta atcctgtgcc
     1861 acttgattta gtagtaaagt tgggctcaaa aatatgttct cttttttcgt tggcaattcc
     1921 aacgccatta tctttaactg tgattaaata attatctgac gctactaata aatgaatgtc
     1981 aatcaatcca attttatttt ctggaatggc ttgaatagca tttttaataa tattggtgaa
     2041 cacacgcatg agctggtctt tatcagcaag cacaacacaa gcagtgcttg ggtaatcaag
     2101 tgaaatattt atattttctt cttctcgaaa caactcaacc gttgattcta aaatcggaac
     2161 aatatcaatc ggtttaaaaa caccttgagg catttttgca aagtctgaaa atgcagtggc
     2221 aatgccggaa agattatcga tttgctcgat tagacggtgc gaaattttgc tcgtcagttc
     2281 agtaatatct ggcgaattat cttttaaagc acgctgtaaa tgttgaatgc ttagcttcat
     2341 tggtgtcaaa ggatttttaa tttcatgtgc tacttgtttt gccatttcgc gccacgctcc
     2401 ttcacgctct gactttgcta ataaatctgc acttttctcc aattcatcta acatcaaatt
     2461 atattgatgc actaataatc caatttcatc ttcatttttc catataattt tcttattgaa
     2521 ttttccaaat tgaacatctt taatattatc agaaatcaat gtgagtgagt tggtaataga
     2581 gcgtgacaaa aacacggcta gcaaggctgc tgctattaaa aacacgacat acacattgac
     2641 caatgcgacc aagaaataag atatatcttc ttgaaaagat ttttgtttgc cgtaatatgg
     2701 aaaattaaaa taacctaaca cttttccatt acttctgaat ggttggtatg cacttaagta
     2761 tgacaatttt ccaatttttt catcatgcac atatttagat ttttctttta caattagatt
     2821 gtaataggca tcagcatcca ttttcgaaga caccaaattg cgtttaaaaa tttctggttg
     2881 tgatgattga attaaatcgc cttttacatt atatacgttg atatcgagtg cgtgtatgga
     2941 agagagtact tttatttttt gttgtattat tttatcaaat gtattatcgc cataaactgg
     3001 atattcttca acataatatt gtgctgtatt tttcattact gcatttactt ttttcaacaa
     3061 tttgctattg tgataaatat tatattgata ttgaaaataa accaaagtaa caatgcctat
     3121 tatagccaag gagaacaata ctaaagccaa cattgaattt tgaatttgct tttgtaatgt
     3181 attgccttta aaaaaattac gaatagatgt ttcgtcccaa aatctatctg aaaaaccaaa
     3241 taaatccatg cataaaaaaa atgctaaaaa gaaaatgacc atgtaagaaa atacagacaa
     3301 agtgcttaat gcaggtctat ttttttcaga caataccact tgcttctctt tactaaaata
     3361 aatcatgtgt gtataatcat catttttata cacagaaaat tctttattct cttttacagg
     3421 aaaattgaag gtcacttggt attcgtaatc gcctttttgt tttactaaat atttattgtc
     3481 ataaatcgca taagagaaat tatcaaattc ttcatcgaag taatttttat gttttaaaag
     3541 taactcagga taggcactgt aggagctaaa tattttcgga ataaactcta caaataaata
     3601 gccaatattt aaggaatcat cttgtatagg aaaatagcct aagtattttt cgccattttc
     3661 tttaatggac aaataataat attgcgacga aatacttttg gcttttcgag aatgtcgagt
     3721 attatttaac ttataaaatt ctttttgcgt gacgccgatt aatggcatgc catctttgtc
     3781 aaatgcatac gtattaatcg tgtagcgacg aagtagtggc ttgaaatatt ttgaagtaat
     3841 tcgattttca atatttactg ttgacaaata tggattctta aaatacgatt tcacaaaatt
     3901 gtcttgcagt atattatttt ctgattcggt gagtgtatat tcttcgccaa tatcacgttc
     3961 ggacactagc tcttccattc tgtacttccg tttttcaata tctttttgat ttgtattgac
     4021 aataataatg actgaagtta aaaaggcaat aaacgacaat aaaatcaagt ttgacaagaa
     4081 cttgtatttt ttattttgtt gaaaaaatga tttctcataa tagaaaaata aaacaaaggt
     4141 gagcaaccat agcaataaaa aacaagcata taacacatct gtaaataagg tcaatttaaa
     4201 gccaatcaat aatccaaaaa cgccaaatac aaaaacgtat ttaaagaagt gcttgtctat
     4261 attgtgttca aaaatccact tcatcaaaaa caataaactt gaaaagccaa agccaaaaac
     4321 taataaactg atgaacgtgt atctatcgag cgtatttgct aaatagaaat taaagctgat
     4381 gacggaattt tgaatgacac tttgaataat aaaaaccaat aagaagtaaa cggcaatagg
     4441 caatatcaac agccaaactt tgtaatattg cttgttaaaa tctgtttgaa taaaacgaat
     4501 gaacgaaagt gaccacgcca ataaaaacac acgaacaagc aaggcgccta atgaatcgcc
     4561 cagatatgca gaagcatata aattagatga aaaaagcaaa gatgttttag tgagtgaaaa
     4621 caattgcaac tcaacaaaca taacatcagt tgccacaatg actaacacaa tgaacgcaaa
     4681 tgccaattta ttttttcgtt ttacaaatgc atttttaata acttggtaca ttgcccaaaa
     4741 aagtaacaac aagccgatga gttctacaac gatgatatat ggattataca ctttttgcgc
     4801 atcgtcagca atacgaattg aaaataaata agtgccatta atgctattaa ttcggcttga
     4861 agtttttgtt tctttacttg aaatcacaaa acgttttaga aatggatgtt tccatacaaa
     4921 gccatttatc aaaaatttat tatctgtagc atactggtga taaattggta tgagcgcata
     4981 caattgtaca ccatcgctga ttttattatt tagcacttca taaaatccat ttccttcttt
     5041 taaaaaataa atatcgtgtt gcgataattt gtaaatatta tctggaataa agctattatc
     5101 agaccaatag tttaaaattc catctttgta tatatatatc agtatatctt tgtcattatc
     5161 caagtgttga atcgcattgc gcacggctgc atattcattt gtagcggcaa gcacttgctg
     5221 attgtgcaag attgcatttg cttttttttc tgttttatgc aaacgtgttt ctatgatact
     5281 tttagcacga ttgatggctg gcgaaatact ttgcaaagta tctaccaaca atccgacgga
     5341 cagcaacaca caaccaatga tggtgataat gctaatttta tgtcgatata aaaactgcac
     5401 ggtttgctaa gctttgttta aaagttcttt ttttgctaag atccaaattt catctaattg
     5461 ttccaaattc atggcatgca tatctaattg tttttcttta gtataatttt ccatccattg
     5521 aaaacgctgg ataaatttac tatttgtctt ctccaaagcc atttctgcat cgacatcaat
     5581 aaaacgagaa taattaacca gtgcaaacaa tacatcacca aattctaatt caatttcttg
     5641 tttattacct tgctcaactg cttcctttaa ttcagcaatt tcttccatta ccttgtccat
     5701 tacttgctct ttgcgctccc aatcgaagcc aactgcacgt actttgtctt gtatgcgttg
     5761 cgatttattg atggctggca aagaattagg cacaccttcc agcaccgatt ttttgccttc
     5821 tttcagcttc aatttttccc aattgcgttt cacttcctct tcgtcttcaa cagtcacatc
     5881 accataaata tgcggatgcc tataaatcaa tttttctgaa acactattca acacatctgc
     5941 tacggaaaat tgttcctgtt ctgttccaat tttagcataa aatacgagat gcaaaaacaa
     6001 atcgcctaat tcttctttaa tagatttcca atcttcttta ataatggcat cagaaagctc
     6061 gtatgtttct tcaatggtga gtttacgaag cgtttgcacc gtttgttttt tgtcccaagg
     6121 acatttttcg cgtaaatcgt ccataatatt taataaacgt gcaaaagcta ctaatcgatt
     6181 gtccataatt ttctttctac aaaaatacta ctttaaaaca aatagttaat ttagtgctag
     6241 aatttagaaa tatgaaaaaa ttaatagccg actataagaa tattcaccct attaaacaag
     6301 gtgtgttttt tcttttgctt atcagtttat ttgctgtaag ctgcctaata ttcaatatta
     6361 aaaatatgat ggcatggaat atgctcattt tagcatttat gttgttttgt gtgttcaatc
     6421 cagttatatc cacattccaa caaaaattac tccaatattt tctgttttca attcctgttt
     6481 ttattggctt attgttttat gtttattttg tcgggaattt tatctctaac atcaactata
     6541 atcaatcaca tgaattacat tttattgcat tattaatcat catcttttat gcgcttgcca
     6601 ttatgtttag cctaatattc agaggtgttt tgcatttttt agaaaatgta gataatcaat
     6661 aataataaga attaaataaa caattattct attcttgatg tttattttca atacttttat
     6721 catacagaaa agcacatgac aagggcagcc attcgaaata ttattttact agcatttata
     6781 tcactggcag gcattattgc tactcaatta ttttggataa aaaaagcgat tgatttaaaa
     6841 gaacagcaat tcaatcatcg tgcgtatgtt gcattaaaaa gcgtggcaga ccgtcttgtg
     6901 ttagacacca aatatagagt gcaattagat ggcatcaata aaaaggcatc taattattat
     6961 acattagaat tcagctctcc agtaaatgtt acgatgctag agaattattt agtgattgaa
     7021 tctaaaaacc aaaacttaaa tgccgatttt gaatatggta tttttgaatg cactaccgac
     7081 tcattttatt atggtagata tgtaaatatc aaaaaaactg gcgagattga acgtttaaaa
     7141 gtaaaagcca agccatcaga aaattattac ataggtgttt tatttccaaa aaagaaaact
     7201 tatctgcaag atgatttaaa cttacttatc ttcttctctg ctgtgttatt agtggtcatc
     7261 ggatttttta catataccat tcttgttatt ttaaaacaaa aaaaattaag cgaaataaaa
     7321 acagattttg tcaacaacat gacgcatgaa tttaaaacgc caatttctac aatttcgctt
     7381 tcctgtgatg tattaatgcg cgacgatatc acaaaaaatc ctgagcgact gaaacattat
     7441 gcaaccatca ttagcgagga aaacaagcgt ttaaaaagcc aggtcgaatc cgtattacaa
     7501 gtatcacaaa ttgaatcaca taaaattgca ttaaattatt ccgaagtgaa tatgcatgca
     7561 cttatcgaaa atattgccaa aaatattaag ccacgtttag acaaacaaaa tggtcatatt
     7621 caattgcatt tatatgatca aaaacatatc gttttaggcg atgaagtgca tttgaccaat
     7681 attttatata acttgctaga taatgccatt aagtactgcg ataaaattcc tgaaattagc
     7741 attaccacag taaataaaca taaaggaatt gaaatagcag tgaaagacaa tggcaaagga
     7801 attgaaacga gttcgcattc aatgattttt gaaaaattct ttcgtgtgcc aagcggtaat
     7861 atacatgatg taaaaggatt tggattaggc ttgttttatg tgaaaaatat gatcaaattg
     7921 cataaaggaa aaatagcatt acacagtgtt cctacagtag gtaccacttt tactatatgg
     7981 tttcacacta ttgaaaaata aacacaatgg acaaagtcaa aatattatta gtagaagacg
     8041 acccaaatct tggttttgta gtgaaagaca acttgagtga caaaggttat gaagtgacgc
     8101 attgcatcaa tggattggaa gcagaacgcg tgatttataa agaaaaattt gacatctatt
     8161 tgttagatgt gatgattccc aaaaaagatg gttttacatt ggcacaagaa ctcagagaaa
     8221 aaaaagacga cacgccaatt atcttcttaa cagcacgtga aatgcaagaa gacaagctaa
     8281 aaggtttctc tgttggcggc gatgattata tcactaagcc ttttaatttc gatgagctta
     8341 cgatgcgcat cgaagccatt ttgaaacgaa caaaatcaac aactcctgag caaaaagaat
     8401 ttaatatagg caaatatatt ttaaaggtgg acgaactaaa gctcatcaat ggcgatgaag
     8461 tttcgtcttt aaccaaaaaa gaagcagcac tgttggagtt cttagtaaag catgtcaatc
     8521 aaccagtgaa acgcgaagat atgctggtaa aaatatggaa agacgatagt tactttgccg
     8581 gccgaagcat ggacgtttac atcactaaat tgcgtaaata tttaaaagac gattcaaaca
     8641 tcgaaataca aaatgtacat ggcgtcggtt ttaaattaat cgacaataag ttgtaaattg
     8701 tgcgttttat cttttacatt ttacttattt tattacatta tcacacttat cctttcacgc
     8761 tattccatct ttctattatc aaaagtgtaa gaaatattca aatagaaatt tctacctttt
     8821 cttggaatat tattccaatc agcataagtt gaataataag tatcagccat attttcgata
     8881 cctactctaa aatgcaattg gtgcgctttt ataggtaatg aatatcctgc tgcaaaatta
     8941 actatcgcat aagcgggctt tgctttttca ccataaaatg cactatgtgc atattgtttt
     9001 gatgcacctt caacgttcat ttccatctct agtcctttta tattataagt taatcctgct
     9061 ttaaaactaa aaggtcgtac caatggcata ttttgtttat taaaatctct accaaaatta
     9121 taagacaatg ccactctagt ttttactgat ggatgtggac gatattccat gccacattct
     9181 gtgctcacta tatttacaga ttctagtgct tgatattctt taacgccgtt tgcgtttaat
     9241 gtcatcgcaa tataattggt attcggaata ccgatgatat aatctaaaat atggaaataa
     9301 tttaatccga aattccattt tatttttttc attttataag caagtgccat atgcaactca
     9361 attgatttct catttttcaa atacggattt cctatataat cataagcatc aaagctattg
     9421 aataaataaa aaccataagc ctctgatata gatggagcgc gctcgccata tgccatcgct
     9481 attgaatatt cccaattatt tttggctaat gtataagcag tttgcaaacc aacaataatt
     9541 ctattttttc cagctgacat tgtcgggtaa aaaatacgca tactgtttaa tccaaattcg
     9601 ctggcaacaa aatttctatg aaatccaatg gacgcaccgt atttaattgt atgcattggt
     9661 ttccatgaaa agttattcgc taaattgaca cctgcataaa ttgtatgcac atcaggccaa
     9721 gtgtacataa acatactttt gcgtgatgta tcatttggat acatggtcat ttcagctttc
     9781 gatttattcc aataggcatt gagcgtcaat gccatctttt gacgcttatg cgctccattt
     9841 atttttgaat acaaacctga cgttgtgctc caacctggca tatccatgtg tataggcact
     9901 attggacgtt tggtatcgtc cataatatgc gttatggtat taaaatatac tcttgtcgtc
     9961 cactcatcaa taaatgaatt aatgtaggaa gcgctatgtt gtactgatgc aatatacgca
    10021 cgtgctaatg ctacgtccat aggtaaagca ggatagccaa tatttacggc attatcaaaa
    10081 gtaaatgagc ccgtaagtga atttttatta tttattttta caccaataga cagagatgtg
    10141 ttatattttt gaaattgtga aaatggaatc aatgttcttt tgccagcttt ataattatca
    10201 gcatctctga aaataaaatc agcatcaaca aagaatttat cattgctgta tttcactctt
    10261 gtggctgctg taaataaatt actattggat tcatatccaa aattgactga gcctagccaa
    10321 ccgatatgac aataatcgtg tttctttttt atcaagtcaa tagcgccacc aattgttggt
    10381 ccgtacgaag caccttcttg tcctgagctt acttttacat ctgataaatt tgaaacatct
    10441 acataagaag taatagggtc cattttatca gtacaagcac caaaaatatg catgccatca
    10501 attgtcaatt gcaatcgttc gctcgacatg ttattcaaca ttggctccca agcataacta
    10561 cctcttcgca ctaaagacac attcatagat ttatctaagt attcatcgat tgatgagagc
    10621 ggtttgtttt gtttggtata attaatgagt ggcttactaa ttattgctac ttcatctaat
    10681 tttacagata atatagaatc tatgttcgta gtatcttgtg cctttatatt tcctgatatt
    10741 acactcaata tcaaaaggaa taatatctta tttttcataa aaaaaattaa aaaatggagg
    10801 agttggaatc ctccattttg taagaaaaaa ctaaaattct aattctaaat ataggctgct
    10861 actttccaca cttggagaaa tttcttcgcc tttcaatact acatctgatg catttttgac
    10921 aataaaattc aacttccaat agccagtcat gctaagtgaa accttaccgt gatacaaatt
    10981 atcagttgta ttaataatag gttgaacatt atttggtgaa gtgtgattgc ccatgtccgg
    11041 cattctcgga tcgaacagta ttgttgcatc agtcacggca ggatatgaca tacttgtttc
    11101 ttttacaaac aaaccaagtg tcaaatcatt tgttgccaca gctgggtttt ctggcgacac
    11161 taacgctaaa atgtattttt tagaatcgct acctgtaaaa ctcgctactc gtttttttgt
    11221 agaattagca accgcaaatg tatctaacac agcagtagta atacttggcg tttgcacatc
    11281 tattgacaat gtccattttt ccatatcgtt tcctggcatt tgaaaaacga taaaaccttt
    11341 atataaaaat tctttacctg caacttttgt aattggtgat tttggtgcag cgtgcgacat
    11401 catggtcatg tgcataatag gcatccaatc taatacggca ttactgacaa actcatcatt
    11461 taatttattt tttactctaa agaatatgtc attatatcct accgttagcg ttccgctacg
    11521 tgtgtaaact tctatctctg tcgtgtcatt gaccatcttt tgtaataatg ttaaacctgc
    11581 taactcatca ggttgttgag ctgtttcttt tttgcaagaa atcaatatta ttgaacacat
    11641 agctattgct atgaaaatga tttttttcat tggtaattta atttgaatta aaaaataata
    11701 gagataatac atgtatcaac aaggcattta taatgcaatg caatactatt aaaaggctgt
    11761 cttatactat tttacaaggt ggatgaaaaa taacactgct gaatgtatag gtataagaat
    11821 caatgaaatt gcctagttta atatcagtgg aaatatcatt tttaataatg aaaattttat
    11881 ttgtgtgttt ttggcaaatt agtgaaatca gttcttctgt gctatttttt gacgtcgtag
    11941 atttagcatc tttatcatca aaaaccttat ttagattttc gtttaaatgg cattttccgt
    12001 gacaattttt ttctggatta tttttattaa tacacagcac tttttgaatg taatcgtaat
    12061 taatagcata gtccattacc ggtaagatag gtcttaataa tgttgtaaac gaaactatga
    12121 ttacgattat aaatttcaca attcgaaatt agaaataaaa acaagattat tctatgataa
    12181 aagtcagcat gaaagatgag attatacaat taacaacaaa ataacaatag ctgtatctga
    12241 ttagaattgt tgcttgtttg ccaataaata catcactgcc atacgcacgg caacgccgtt
    12301 ttccacttgc tctaagatga tagaatgttc gctatctgcc acatcagatg aaagctctac
    12361 gcctcgatta attgggccag gatgcatgat gataatttct ttggaaagct tatctaatcg
    12421 ttgtttattt acaccgaaat acaatgcata ctcgcgcaat gatggaaagt attttgtgtt
    12481 ttggcgttct aattgaatgc gtaaaacatt ggccacatca caccattgta atgtttcgtc
    12541 taagtcgtgt gacacgttta cgcctaatcg ttctatgtgt ttcggtataa gtgtagcagg
    12601 accacacaga gttacctctg cgcctaattt tttcaaacag aaaatatttg acaaggctac
    12661 tcgcgagtgc atgatatcac ctaaaatggc aattttttta tttttcaaat cgcctatttt
    12721 ttcttgcata gaataggcat ctaacaaggc ttgtgttgga tgttcatgtg tgccatcgcc
    12781 agcatttaca atattggcat ctatgcgttc tgataagtag tgtgctgcac ctggacttgc
    12841 atggcgcatc actatcatat ctactttcat cgacaagata ttatttactg tatcgagcaa
    12901 agtctctcct ttttttacag acgatgatga tgatgaaaaa ttaataatat ctgcagacag
    12961 tcgcttttgc gcaagctcaa atgaaataca tgaacgtgtt gaattttcaa aaaacaaatt
    13021 agcaatcgta atatctcgaa gtgtcggtac tttttttatt gggcgattga taatttcttt
    13081 gaattgcact gctgttttta aaatcaactg tatgtcatct actgttaaat gctcaattcc
    13141 tatcacatgc ttcgaactta actgactcat agttgattaa aatatgctat ttttttacaa
    13201 aaattacttt gttattaatt tgttcttcac ctgcccaatg cacctctaca tctaccgatt
    13261 caatggtatc gactacactg cctacaaaat caggttgaat aggcagctct ctgttaaacc
    13321 gtctatctat tagcaccaac aattctattt ttgttggcct accaaatgac aaaagtgcgt
    13381 ccatcgctgc acgtattgtt cgacctgtat ataatacatc gtcaatcaat aatacttcct
    13441 ttccttcaat atgaaaatca atttcagtta cttttgcgtt cagcgatttt cctcttctaa
    13501 aatcatcacg atgaaaagta gtatcaattt ttccaaaagg aatttgtacg cctgtaagtg
    13561 atataatttt ttgatgaatt ttttcagcca acaaaacacc tcttggctgt acaccaataa
    13621 ttactgtatt agtaaaatga atagattcaa tgacctctct gcttaatcgc tcaatagtca
    13681 actgtatttg gtcgccgttt agaagtacag tatccgacat tgttgcaaag ataatgaaaa
    13741 atcagacaac tttataagga aaaatcatgt atcaatatta acaaattgaa attgatatat
    13801 attaacaact ttctatcgct ttaattcgtg caatgcctta tttttgcaaa aatatttagc
    13861 tatgcataag acatttggaa ttattgctgc actatacggc ggacttgctg tggtactggg
    13921 tgcctttggc gcacatgcgc ttaaaaacaa actcgatgcg tatcagttag atatcttcaa
    13981 caaaggtgtc caatatcaat tttatcatgc tttagcatta atagcagttg tattgatttc
    14041 cgataaatta aatacaaaaa ccttgacaat agcaggctgg aacttttctt taggtgtact
    14101 tttctttagt ggttccttgt atttattagc tatgcgacat cttttaggta tggaaggatt
    14161 cgcttcaatt ctgggaccaa tcactccatt aggtggactt tgtttcatta taggttggat
    14221 atttttattg atttcttttg taaaaatgta agaaaggaaa ctattctgca acttatttcg
    14281 ttacgtttta atactttatt cttatttatg aaagttagct ttactatact tatattctta
    14341 cttttgtcaa ctacatttgg gcagccacca cagcaatttg gcacagcagc tggctcgata
    14401 agtgggaaag tagtggacgc agcaaaatcc tcacctttag cctactcatc agtaacctta
    14461 caaagtttaa aagactctgc aattatttta ggctctttag tagatgaaaa aggatttttc
    14521 gaaattacaa aaattccgtt tggcgcatat caactcaccg tttcatatat tggatatagc
    14581 gattataaga aacagccgat tgtagtggcg ccaccagata aaatgagtgt caacttaggc
    14641 actattaaag taagtgaaga cagtaaactt ttagacgaag tacaaataac agcagaaaaa
    14701 agctacatgc aagttaatgc agaaaagaaa gtatttaaca tggataaaaa catattatct
    14761 actggaggtt ctgcgattga tgccttaaag caagtgtcct ccgtagaagt agatcaagat
    14821 ggtaatttat cgatgcgtgg ctcaggcaat ttgcgcgttt ttataaatgg aaaaccttca
    14881 ggtattaccg tcacaaacac aaaagcaata ttagatgcca ttcctgcatc acaaattgaa
    14941 agcatagaag tcatcaataa tccatctgca aaatttgatg cagaaggcga agttggcatc
    15001 atcaatattg tgctgaaaaa aaatctgaaa aatggattga atggaaatat tacaataggc
    15061 tacggcacta aatatgatgc caatacaggc gccaccatta gctatcgaaa aaataatatc
    15121 agttttacta catcatataa ttttagattt acagaaagct attttaaagg cactagccaa
    15181 cgatttaatt ttttccctaa tcagacgcct ttttatttaa acaatagtga ttatggaaaa
    15241 taccacacat ttgccaacac aattaatagt agtttggatt ggagcatcaa agaaaaaaat
    15301 acgctttcat ttagcgtttt acttagcact tctaacagta aaacaaatgc tagaaccagc
    15361 acacattttt tagacacttt agtcgagtat gtgaatgcct acaatcgcta taacaaagac
    15421 aaacaattca attggaatgg cgaaataaat acttcgtata gacgaacgtt taaagacaat
    15481 tctaacgatt tggttttggc agcaaactat gcctattccg acagaaaatc atcgcctatt
    15541 tacacgcaaa aaacacttga tgtaaatgat gaagagcttg aaaatattga cttattacaa
    15601 cgcaataaac atactaacat tacgcataca gcatttgctc aagctgatta cacacagcct
    15661 tttaaaaaat caaaatcgaa acttgaactt ggttataaat attcatttag aagtattgca
    15721 acagcattaa atggcgattc attgctcagc agcacacagc aatatgttta tgatagttct
    15781 atttcaaatc aatacaatta tcgcgaacaa atacatgctg catatatgat ttttggtggt
    15841 gctgtaaaaa aaatatttaa ctacaaagcc ggcattcgcc ttgaaaatac agctttaaac
    15901 atccatcagc aacgcgattc tgtcatacac aaacaaaatt atttcaatta ttttccttcc
    15961 gcatcttttt cttttaattt accaaaatca catgctttga gtgtgtcata tagtaagcgc
    16021 attaacagac ctagtgcaga gcaattaaat ccgtttggta actacagcga tccatataat
    16081 attcttactg gcaatcccaa catgaagcct tcgtacacac atgcagtgga attgacacac
    16141 gtaaaaaata ttgacataaa accttctaaa cgagattcaa catttaacag aagtgtattt
    16201 ttcagtacaa caatttattt cagacatgct tataatgttt ttacacgctt tagaacggtt
    16261 gatagttctg gacattcgat tgttaatttt gataacttaa gtagtggcca aaatattggc
    16321 cttgaattta cgaatagaac cagtttattc aaatggtgga attttgtatt gacaggaaat
    16381 ttcttttata attcagtaaa aggcaatgtg cctaatggcg aagttgatgc aacgacaaat
    16441 agctttcaat acaatttacg cttgatgaac acttttaata ttacaccaaa agcatcattg
    16501 caatttatgg tgatgtatcg tagtaaaata aaatttttac aaggcgaaat aacgccaatg
    16561 gtatttgcaa atattggttt caaatatgat ttcttaaaaa ataatagagc cagtattgct
    16621 attaatatta gcgacatgtt tcatacacaa tattttggtg tgcgtacaag tggcaacaca
    16681 tttacagcta caacttctcg ttattgggaa tctactgtag gaaatatagt atttacgtat
    16741 agatttggta aaagcgatag caaaaatgcc attagcaaac agaagaaatc aaactttgaa
    16801 gacagtggaa atggtgttga aggtggcggt ggatagcctt agtttttgcc aaactttaaa
    16861 gtgtacataa agtctttaaa ctcttcatct tttgcccagc caacaaaata tttatctttg
    16921 gcgatatagc caaaatacct attatcattg aacaatccag ctttttttgc ttcgcgtaag
    16981 taagtaaatg tgttgttttt ttcgccttca gcagcatagg ttcttgcaat ttggtaatat
    17041 gcatacgcgc gctcatttgt caactgcatc gacttataaa tatcagaacg tgctgatttg
    17101 aaattttcta attctagctt acacatacct ctatctaaat aatattccga tttataattg
    17161 gttaaattaa tagcattgtc aaaaaactga attgcaatcg tatagttttt ttgttttacc
    17221 aaaacttttc ctaaataata ttgagcgctt tcatcattag gatcgagttg tgttgcatat
    17281 aataaatcat tgtaagcttc atcaatctta tctaaatgat atcgaacaat tcctcgattt
    17341 cgatttgcca attcaaaatc tggttttagc tctaatgact tattaaactg agcttctgct
    17401 agtttcaaat ctactacgct tgcctctgca atattttgat tgttatatgt tgccgagcca
    17461 atattattat aggcgatata attatttgga tctagtttta ttactcttct aaaatcttta
    17521 atagccagct caaacttatc tgcatagtaa tagcacatgc cacgactatt aaataaatta
    17581 atatcagagt cattcatctt aactgcttta ttgtaatatg gcaacgcgtc gtcaaatttt
    17641 ttatctagca ccaacatatt tcccatatca acataagctg cataacactt tggatgtagc
    17701 ttgagcagta aggtatagtt ttcatttgct gcttgaaaat ttttcaattt ataaaagcaa
    17761 aatgccttcc aatagagtgc aacagtatca tttttatgcg attctaaata ggaatttatc
    17821 gtggaaatgg caggttgata gaatttttgt gcaatttgtt ccttggcaaa ttctaaaaca
    17881 agatctgttt ttttacgagc gaatgtatta tttgaaacaa gaaaaaaaca aaaaaacaag
    17941 cctattatcc acgcttttga taagtatgat ttagctttat tcagcttggc tacttgtaat
    18001 attttgttca cctaatgata gtttatgttt ttccactaca ataatagaaa aatcttgttt
    18061 ttcaattctt ttaaaatttc cactattgtt tatagtaata acctcgtaat tactcggcac
    18121 tttaatgtat agtgtaactt catttaaaag tcctgatgtc cattccaatt ctatattatt
    18181 acctgtaatg tagatatttt caatttccgt gccagaaaag aaatggcctt tactgccggc
    18241 aattgcaaat ggtgtgtagc caaccgtaag caagcaacgc gaagtgtgct taggaatttc
    18301 aatccgctta tcgcctttca gcaagtcttc ttttacgtta tcgaaatata aattagaagg
    18361 aagtttgtac atttttgaag aatcacttgc attaatcagt attaaataat atctgtcttt
    18421 tacatgaacg tttattgtat agaaattatc ttgttcatgt acttgtgcat ctttaattgc
    18481 cattaatgga aaaatagatt tataccaaaa caattgctct tggtcgtatt cggcaatatt
    18541 atctgatgta aacaatagat gtccgaataa taaattggca agcaacaagg aatatttctc
    18601 ttcaaatgac aagctatttt tatttttcct taagagaaaa acatcaggat cattccaaaa
    18661 ccattttcca gacaaatgcc ttcgggaaat agtgttgtgt attgcattta atactgatgg
    18721 acgctcgcgc gcgcgaatcc attttaacaa tgaaaaatcc cagcttaaat gtatgtcagg
    18781 ccctattctg caatattccg tagtgccatt tgcagcactc aaaggcacgc cgcaacctaa
    18841 tatcattttg tcgcctacgc acgcgcataa aaatgccatc gcatcgtgca taatttcgcc
    18901 tctcgatttt ccatttctgg cgataagagc agtaccatat agaaaatcta attttaccaa
    18961 atcaaatttc cattcgtgta aaatagtttt gaaaaccgta cgcaaatacg tgcgcacttc
    19021 ctcattatat acgtctaatg catagaacca aaagctccac agcggattga aacctatttt
    19081 taaaaattta tttttatcat ctttcaaaat ccaatgtggt ttttccttta caataaatga
    19141 attatgttcg catgcaaaag gtgcaagcca aagtcctgct ttcattttat gtttgtgcaa
    19201 gtcctctact attggcagta atccgttcgg aaatttatga ttaatattta gccaatcgcc
    19261 aaccgcttgt tgccaaccat catcaatttg aaagatagaa atcggaattt tattatcgac
    19321 aaaagctttt atattttcta ctattacttt ctctgatatt ttagtatagt aataatacca
    19381 actcgtccag ccaattgcag gttttgcatg gattggttta atatgttgag cttcaaaata
    19441 attttgaaaa cattgccatt cataagtatt tgaagcaaat acatctataa tattcaagct
    19501 tgtgttggct gccaattgca taccattgca gtcacttata atttgcatgt tgttttgtga
    19561 tgcatcatgt tcaaaaatag tatatgattt tgcatcactc agagaaccta aaaaaaatat
    19621 tttacgctta ggtagtttta tataagtata tgtccaacta tggaatacgc caactttatt
    19681 tttataatta aaaaaagtac cgtcaccata tgcctcacca attattttaa cgccacggcg
    19741 ttgcgattgt aatttctcct gcttgttaaa taattttgtt tctgtccatg attgaaaacc
    19801 attacaaaaa atgctattgc ttgctgaata atcaaaatca gcatttatat aaaagtactg
    19861 aagctcaatt ttttctttag cattgaaata cacgacatac cgcttatcat ctcctatttc
    19921 ttccaatata aattctattt tgagatgaat attttcaata atggaaaaat cgcttgtatt
    19981 aatctcgaat ttttcgtttt gagcagtagt gtaacttaga tgaacgtttt taaatttcat
    20041 gtaagacaaa gataaaatat tccgagttta ttatagcttc gtttatgtag atgaaaaata
    20101 taataatgaa aagcacataa cacttcaata ctgtccactt ttatcttgga attccaatag
    20161 gaactccata aattatttgc agtaaaaaag gcagtaaaat actaaattct tcaaattacc
    20221 aaaaaaaaat aagccaccat gaggtggctt tagtgaccgc ggcacgaatc gaacgtgcga
    20281 ccgactgctt agaaggcagt tgctctatcc cctgagctac gcggccaagt gttgacaaaa
    20341 atcacattaa atatataatt ttcagcaacg gagtgcaaag gtatctataa attgagtaat
    20401 ttcccaaaaa atcactttaa aaaattatgt ccgaagtaaa tattcaaatt gaagacagct
    20461 ggaaagacgc tttaaaaaga gaatttgagc taccctactt tacggagtta cggcagtttt
    20521 tgcatacaga aaaacaagaa ggcaaaatta tttatccgcc aggaacgcta atttttaatg
    20581 cttttaacac cacaccatta gcacaagtaa aggttgttat aattggccaa gacccatatc
    20641 atggcgctgg tcaagcacat ggtttatgtt tttccgtaca acacggaatt aaaccaccac
    20701 cgagtttagt caatatttac aaagaattat atgctgatgt tggatttaat attccgacac
    20761 atggctgcct ccaaaaatgg gcagaacaag gcgttttttt attaaatgct atacttactg
    20821 tagaggcaaa taaaccagca tcgcaccaaa aaaaaggctg ggaaaatttc acaaacgccg
    20881 ttattcaaaa actttcagaa caaagaaatg ggcttgtttt ccttttatgg ggaaattatg
    20941 cacagcaaaa agcaacatta atagatgaga agaaacacac tattttaaaa gcagcacacc
    21001 cttcgccatt ttctgcctat aatggttttt tgggctgcaa acatttctcc aaaacgaatg
    21061 aaatattaca aagtcaaggc attgctccga ttgactggca ggtttaaaat taaattatag
    21121 atacgtagaa atttatatct ttaaagttat atcctttcaa aattacaaaa tctaaaaaga
    21181 aaaaatatta atacatttac aacatggctt taaaaaatat ccgatataat tttacacccg
    21241 ttgagcattg tatcatgtgt ggtgagcgtg tggctacaca taaggttttg gggaaacgca
    21301 tgaatcaatc gcaaggtaaa aatcctaaaa ataaagttgg tattacaaca tctgtactgc
    21361 aatgcagcaa gtgtcattta atttattcta atccactgcc tgttccgttt gacatacaag
    21421 atcattacgg tgtgccacca gaatcctatt ggaaagaaag ctacttcgta atagatgaaa
    21481 agtattgtca aaaagaaatg gatcatctaa agcaattaat ggatattaaa cccggaatga
    21541 aagccttaga tgtaggtgct ggtttaggta aaggcatgct tgccatgcaa cgcattggcc
    21601 ttgacgctta cggattcgaa ccatcagaac agttttatga aagagcgata cataaaatgg
    21661 gaatcgcacc agaaaagcta aaaatcggca tgatagaaac cgttgattat ccagaaaatg
    21721 aatttgattt cattatgttt agtgctgttc tagaacacat ctatgatcca gcagctgcaa
    21781 taatgaaagc catgaagtgg ttgaaaccca atggtattat tttcatcgat gttccatcag
    21841 cgcattggct aactagcaag attttaaatt tctattatag aattactttt tcagattatg
    21901 tatgtaatat cagtccgatg cacgacccat ttcatctgta tgaatttagc ataaaatcat
    21961 ttgaagcaca tgctcaaaaa aataattatg aaatcatttc atattcgtat gaagtatgta
    22021 gtacttattt accaaaatct ttagatttta tcatcaaacc aataatgagc aaaacagata
    22081 caggcatgca attagatgtc tggttgagaa agaagaaata aagtcttatt tttatggcat
    22141 gaataaaatt gctattttcc ctggctcttt cgaccctatc actattggtc acgtcgatat
    22201 cgtcaatcgt gcgattccgc tatttgatga aatcatcatt gcaatcggca tcaacacgca
    22261 gaaaaaatat cttttctctt tagaacaacg aattgcttgg ataaataaag tgtttgcaaa
    22321 tgcgccaaac gtaaaagttg aatcatatac aggattgact gtcaattatt gcagagaaaa
    22381 aaaagcgaaa tatattatac gtggcattcg ttcttcagcc gattttgaat acgaaaagac
    22441 aattgctcag ctcaataaaa tgatgatgcc tgaattagat acgttcttaa ttctctcctc
    22501 gccggaattg tcagccatat catcgacaat tgttcgcgaa atcatcatcg gtaaaggtga
    22561 tacaagtaaa ttcctgccaa aagaagtgga tatttcaaat gtaactattt aaatcgatta
    22621 tgattttgag ctattttcca ttgatttaca aaatggtaat caatcaatta tttttcagta
    22681 ttagtattat taaaaaaaat cactaaattt gcgttccgaa ttttcggata actaaaacct
    22741 gaatgctttt ttagcaagac aggaataaaa actaaaacat atttttatat ggcaacaaga
    22801 ttaagattac aaagacatgg tgcaaaaggt gctccattct acaaaattgt agcggcagac
    22861 agccgtgcaa gaagagacgg taagtacatc gagcaagtag gtacttacaa tccaactact
    22921 attcctgcaa ctatcatctt agacgtagat aaagcagtaa aatggttaca aaatggtgct
    22981 gagcctacta caacagtaaa agctattctt tcatacaaag gtgctttata caaaaaacac
    23041 ttattgcgtg gtgttaaatt aggtgttgta aaagcagaag acgttgaaac aaaattcaat
    23101 gaatgggcag cacaaaaaga cggcaaggta ttagaccaca aagcaaaagc agacgctaaa
    23161 aaaatagaaa aaaatcttgc ggttgtaaac acaccagcta aggtaaaaga agtgcctgca
    23221 gaggaaactc cagctactga aaatacagaa gaagctggca ccgaagctaa cactgaaaca
    23281 gctgaataag caacataaaa ttgtataaaa tcccggcttt atgtcgggat tttttattta
    23341 agttattaac tttaacaaac aaatactgca agtattaatg gaattaataa aaattggaaa
    23401 aattgctaaa acacatggat ttaaaggcca tataaaagtg tatatcgacg aattttacat
    23461 gaatgacttt gaagatatgc aagccatttt cattaatcag ctgccctatt ttatcagcaa
    23521 aaaagatatc aattccaatt cacaagctat tctattgtta gaagacattg atactaaaga
    23581 aaaagcccaa aaactgcaag gcaaagaaat ttttgctaaa gatgatgatt tgacagaaat
    23641 tttagatgga aatgaatacg acgaacttat taatttcata ttcatggata aatcaagtgg
    23701 caaaaacggg aaaatcatcg aaattataga aatgcctttt caatttttag caaaggtaca
    23761 attcgaagat agagaaacct taattccttt aaacgatgat tttatattat caataaatag
    23821 aaaaaagcac atcgttgaaa tgtgtttgcc tgaaggatta ttggacatta attgattaaa
    23881 agaatacaaa aaatgcgtat cgacatactt tctgccgttc ctgacttaat gcaaagcttc
    23941 tttgcgcatt ctattttgca gcgtgcaaag gaaaggaatt tattagaaat acatttgcat
    24001 aacattcatg actattcgaa aaagaaatat aaacaaatcg acgattatca atttggtggt
    24061 ggagcaggca tggtcatgtc aatcgagcca attcttacat gtattgaaaa attgcaagca
    24121 gaacgcaagt acgatgacat catatttatg acacctgatg gtgaactatt agaacaaaaa
    24181 atggtcaatt cgctgtcctt aaagcaaaat atcatcatac tagctggcca ttacaaagga
    24241 attgatgagc gtttgcgcga acatgtcatt actaaagaaa tatctattgg aagctacgta
    24301 ttatctggtg gcgaactacc agcagctgtc cttgtcgatg caattggaag gctaattcct
    24361 ggtgttttaa acgatgaaac ttctgcttta acagactctt ttcaagataa tttattggcg
    24421 ccgccagtgt acacacggcc gtcagactac aaaggttgga aagtgcctga aatactactt
    24481 tccggcaatg acaaactaat tgcagagtgg cgccaccaac aagcattgga acgcacacaa
    24541 cagcgccgac cagatttatt agaaaacgat taaataacat tgcatttcat aaaaatctac
    24601 gtatctttgc acacgctaaa atctacagcc atggaaaagg taaaagaatt gactaaaaaa
    24661 ttgacagaaa cgaaacaaat ccctgatttt aaggcaggcg acaacataaa cgtacactac
    24721 aaaatcattg aaggctcaaa agagcgtatt cagattttcc gcggtgacgt tattcaacgt
    24781 aaaggcactg gtgcaacaca aacattcact gttcgaaaaa tatcgaatgg tgtaggtgtt
    24841 gaacgtattt ttcctttatt atcaccaaac atcgacaaaa tcgaagtaaa taaaattggt
    24901 aaagtacgtc gtgcaaaaat attctacatc agagaattaa aaggtaaagc atctcgtatt
    24961 aaagaattag taaaaacgac aaagtagtat aaactataat aatacaatac gaataaagtc
    25021 ctgctaaatg caggactttt ttattgcaag tatgtgaggt tccctattta agttttaagt
    25081 gaagacttag tttatatatt caagcatttc tcatttttaa ttattaacta ctaatgtacg
    25141 attcatcaat aatagaaaaa gtaatcaaat aaaatattga atacaaggca aaagaaaatc
    25201 ctcaaaagaa taaatagtaa taaaataaaa aatgcctcca tcactggagg catttttatt
    25261 atttgaaaag attatagatt attcaccact taagtaaata tctacacgac ggttcatcgc
    25321 tctatcttct tctttatttt gtttttcagt tacccaagaa ttttcaccat aaggcaacaa
    25381 caatactctt tgagagatac caacaccttc taagtagcgt ttaacagcat cacaacgttt
    25441 agcagataaa acttgattat aagttgtacc accttcgcta gatgcattac cagctaagta
    25501 aatgaagtag ttacctttca tttccgctaa tttgccttta aatgcatcaa tacttgcttt
    25561 agcttcgtct gtcaattcag ctttgtccac ttcaaagtaa acagaaccaa attttttgta
    25621 cacgatatta tctaagcgtt gagcaacttt ttcgatatca gaagtatttt tctcagatgc
    25681 agatttagct tcattcgctg ttttctctac attagttaat gcattaccta aaggatctac
    25741 tttagcatct acttcgtcaa ttttcttagc gttgtcatca gttttctgat ttactttcgc
    25801 aatttcatca tcagtacgtt ttttgttttt ctcaaattcg tcttgatctg ttttgcgttt
    25861 caatctaaag cttaacaaga tttcatgtgt tgaacctaag ttagcaaatg atgtattgaa
    25921 gcttttcttg ccaacaggtg ctttaaatgt atagcttatg tttacaagct ctttaatgcc
    25981 aacacctgca gttgcatgaa tacctgctgc attagagtaa atccatcctt tatcactgtt
    26041 acctgttggt ttcataccac caccattttt gtaacctaca gcaaaccata ccaatttttt
    26101 atattgagcc attaaggtca agtctaaata aacaggtaat gattttttaa taaagttgat
    26161 ttgtgcaaat ggtgacaaag tccactcttt ctttttacct aatttagcat catagcttac
    26221 catgaacatg tattgacgtt caattctagt atagtgacta aattgaggat ctgtagtttt
    26281 gaatctaact ttgtttccta aaacctgagg aacagagaag ccaacattta accctttcca
    26341 gtggtagtta ataccaagag ataagtcgaa atttacactt ctgccattag ctgtcaatgg
    26401 gtcattcgca tcttctggag tgaagttatg taagcctatt gagtttactc ctgcttgtaa
    26461 gccaaaagat aaaaaatgtg ttttatcttt tttgaacggt aaatgataag cgtatgctac
    26521 gctaccgcca gtattttgcc attgaggcgt tgttctgtca tggtagatag ttacacctag
    26581 cgccatattt ccagacttga tacgtccatc ccaagatcca tacgttgtga taggaccatc
    26641 aggttgtcta tcatgttgtt tttggaatcc tagatttatg cgtccgccat ctaaatccgt
    26701 ccctgctcgg gccggattat agaagaacgg attaagaaaa taattgttaa acactggtag
    26761 ctgttgtgca tccgataatt ggattcctgc gactaacaag gctactaagc tgatgatttt
    26821 atgtattttc attgttatga ttttacttag attattaatt cgcttcattc ctaattatct
    26881 tttaatgtgt aatgcacctt tatacgtttt gtttggcgtt acaataacga accagtaagc
    26941 accttcaggt aaggttgtac ctttatatgt accgtcaaag ccattgctat tgtcgtacgc
    27001 tgttgttgtg taaactaatg caccacctct tgcatatatc tgaactgtat atggtatttg
    27061 agcattattc aagttctcga tatgctgtat aaaccatgag tcgttcaatc catcaccatt
    27121 tggtgtaatg atatctggaa taattaagtc tagagaaggt attacagtaa tttctattgt
    27181 atccttgtct gtacagccgt ttttatccca aattgtgaat accattacgt aaggattagg
    27241 aactggtaat tcagtaaagt caacgtatgg attaggaata ttatcaatat ttactaattc
    27301 attaggagtc cagttgaaag taataccagg taatattggt aatggaccat tatcagaagt
    27361 accattcaat tggaagattg taccttgaat aattgatgta tcatcacctg catgagcaat
    27421 tggattagcc caaacaatta catcagtagt agcagagtca gcacaaccac caggtaaagt
    27481 aactgttact gtataagtac ctgtttggct agggcttgca ggatccaatg taggattttt
    27541 acctgttgct gtccaaccgt taggacctgt ccaagtatat gtagcaccaa atacgtttgt
    27601 agcatttaat ttaattacgc caccttcgca aattggacca tcattatcag caatcacttt
    27661 atcagggaat gtgtagatct caactaactc gctgtacact ttagaagtac aacctgttaa
    27721 tgtatctgta actactaaag tatagaaacc ttggtgatca acttccgtta catcagcaat
    27781 tgaaggattt tgttctgttg aactccaacc attaggacca gaccattggt aagcatatgg
    27841 attaccatct gatacatgtg tagttaattg taatgtttgg tgctcgcaca atggagcatt
    27901 actgctaatg ctatcaactg tcggagcagc attaacgata agtgttactc tagaagaatc
    27961 tgaagtacaa ccatcaatcg ttacagaaac aacatactcg cctgacattg ctgcagtagc
    28021 atctgttatt gtaacacagt taccattgaa aataaatccg tttggacctg accagttata
    28081 cacggcacca gcttctggag tagatgcaca gaatgttact gtacctcttt cgcaaactga
    28141 taaagggcca gcaggtaata ctggagcaac tggtttttct ctgattacta ctgtagtata
    28201 agttgcttca gaaacacaac ttccaattga tgcagttaca ctatataaac cactttgagc
    28261 tggtgtaacg tttgtaattg taaatgttgt atctgtagtc gttcctaata atgaaccatc
    28321 tggaccatac caattgtatt gaacaccagt gccagcaact gtagtacaag tcaatactaa
    28381 atcaccttca ccacatactg gagtattgct attgataatt ggtgtagcag gcgtttctgt
    28441 tactgtcaat accacttctt ctgcagcgct ttcacaatta tctttagtac aagttgcata
    28501 gtaagatgtt gtagctgcag gagctgttac attcaataca gaaccaacat gaatcatatc
    28561 tgttaaagca gcatcgctat accatctgat ttcaccatcg caagatgctg ttaatacggc
    28621 atcatcacct tcacaaacgg ttacatcttc agcaactggt gcctctggag tttccgtaat
    28681 aactaatgaa gcagtaccaa agtctgacaa gcagaagaat ggatcacctg taaaggcatt
    28741 aaaccaaaca tggttaactg tagctccgat atcatatgta ccaggtacag agtaatcata
    28801 atcttcaccg aaaggaccag tacctacttt aggaatcgta attgaaacgg cagattcaaa
    28861 tacgccattc catgggtcta atgcagcagt agctaaatct aatgtagtca atggaccgct
    28921 tactgttcct gtattatctg tgaattgtac gattgcttga gcaaaagtaa atacatcaaa
    28981 gaaatcatca attaaatcgt gagggaatgt aacatgagcc gtaagtgtga ttgtttcatc
    29041 tgactcacaa accgtaacgt catcaactct tggagccggg aactcaggtg ttgcatatac
    29101 atcaacaaag ttcatttggc tatggcaacc tgtaactggg tcaactgcat cgaaatagaa
    29161 tcttgtccaa gatgcgattt ctggtgttgt aaactcacca tttccatcaa agccgaagaa
    29221 tccagtaact tcatctaagc cgcctttatc taagtaccag tgaatttcat caccgttaga
    29281 attagaagca aacaatactg ttgcatcatc ccaacatgtt acatagaaac cttcttccgc
    29341 ctcaggcatt tctggacgct cgtttactgt tactacaact gttgttgtag ggcttaagca
    29401 agtaccatta ttatttacaa catacaatgt agttgtagca tttaaaggtt gtgtagtata
    29461 agtagcacca atatgtaata atgtagataa agcagcatca gcataccatt ctacttgacc
    29521 aattgggtta gttactgtta atatagctga agaaccagca caaacagcat caaaacgagc
    29581 aacaatagga gcagtcgctt gaggtaatac gtttaatact acaggcgttc tgctatttga
    29641 acatgtactt acagaatcac attgcgcata gaaagtatat gcacctggcg ctaatgtatc
    29701 atattggttt ggtaagaaag gagaacccgt tcctacttga gtaccacctg ttggtgaatc
    29761 ccaccatgta agcacgttac ccgcaactaa attatctact gagtaagtaa tctctaatga
    29821 agcagaagcg atttcgccac catctccacc aaataagtca tcaatattca atgtccaaat
    29881 accttgtgct ggtgttgtgt tgaatactgt aaatgtttca tttggtctat atgatcccgc
    29941 aggaatatcc atatcagaac cagaaattgg atttactgta gctgctgcag catcgtcaaa
    30001 cgtataagtc acaacatccg taccagcagt accatagtta tcgctaccta aaccattatc
    30061 attaacgatt tcagcagttg cagcacttgg agatgtaagg cttaagaata cgtcaccagc
    30121 ccaagtatgt gccatttcta cagtagctgt tactttagta actactgcgc cagcaggtaa
    30181 tgctgaagca tcgaatgtaa tagattgtgg atttggagaa ggtccgattg gaacagggaa
    30241 gccaactgaa ggtaaagtac ctccaatatt tgcagttgca gaactacctg atgtataaga
    30301 tacatttaaa gtagcacttt caattacacc agcatcaatt ccgaataagt cttctacatc
    30361 taatgtccaa gagccagaag gatcttcgcc gttaaacgca gacaacgcgc tataagggaa
    30421 ataagtacca gcagggaaat cataagtaga acctaaacca ggtgtaagtg tgtttgggcc
    30481 tgcatcatca aaagtatagg ttgctgaaac agtaccatta cctgtaccaa aattactact
    30541 taaagtactt agccaaacag ggttagttac atctacagat gtagctgcag gtgatgttaa
    30601 tcttaaataa acatcagccg cccaagtatg tctcatattt aaagtaatag acacattgtc
    30661 aattgtagca ccagcaggaa ttgctgaagc atcaaacgtc attgagcctg aataaccttc
    30721 atctggtcca atcgttaaac cgccgccacc agcaacgcca ggtatgctta cagttgtaat
    30781 aacaggaaca acaccgcctg cattttcgca agctgcttga agaccctcac ctggaggaac
    30841 tgtttgtcca agacaaattg tataatcttg cgctgcgtaa ggcatttcag ctggaggatt
    30901 aacgaatact tccacagctg tagcttcgct ttcgcactca ccatatttag ctgttacata
    30961 gtaggtagtt gttgaagaaa gtgctggtgt gttatactct gcacctactt gaactgcatg
    31021 tgttaaacca gcatcatcgt accaagttaa tgttgcgcca tcttcaccag ttgctaccaa
    31081 tgaagtagat tgacccacgc atactgtatc tacagcagcg gttggtgcat ctggtgtatc
    31141 atttatagta agataaaatg gtactaaatc gccatcacaa taaacaggat tgccatctaa
    31201 gtcatcaaaa tagctatttg acaattgtcc atagaataaa tatgtaccag gtgcaattgc
    31261 tggagacaaa cctaacgaaa aggaagcatc gagcccatca aaaggaagaa caccgaataa
    31321 gttaaaaccg ccatcataaa tgactaagtt ggcatcattt gctggatccc agcctgttaa
    31381 agttgtatgt acaaaaaaat caacaaaagg atctccttca caagcttccg catcgtcaat
    31441 agcgatttca ggtttatcca caacatctac tattgctgga ataactggtc ccgtacatac
    31501 tccgtcattt actctaacat aatacactgt cggttgtgta cttgacaaat taaagaaata
    31561 tgttccagca ggaactgttg acgtaattgc aacaaaatct ggatttagtg atacatcgta
    31621 aagttcaccg ccacctaaat cattaattct tatttgagct tctccatcaa aacaagctaa
    31681 agaaacatca acgtcaggag ttggtaattg agggcgaaca gtcacattta ttgctgttgc
    31741 cgcaccaata caaccaccag cttggtccat ctctgctaca taataggtag tgctcgctgt
    31801 taaaggaggc gttgtatatt gcgaaccata agcaattgga gacattaaag ccgcatcgtc
    31861 ataccaagca aacatattac ctggcacact tgcattagaa gtaaccgaaa aagatgcagt
    31921 agcattatag catacctcta catcaactgc agcaactgta ggtgctggaa gtcccgctgc
    31981 tgcatttaca acagacatag taccgaagat ggtataacca ttcgccatag ttctagacca
    32041 tgcaccgtta gctactggaa tattaaataa ttggacaccg ctatagtcat catcaggaag
    32101 agcacaacct gggtcataag tcgtgttgct tccgcaagcg atattatctt cttcccagct
    32161 tgatgctctg atgtttacaa atgtggcgcc acaaactgta gtactaaata cttgctgtgg
    32221 tgttccccat gtgtgtgatc cagccattac attgtctagg cctcggaagt acacaggtga
    32281 ataagtagca tctgtgttta atttaccttc gagtagccat ctagggtcag gagaacctgt
    32341 aaatgaacta cagccaccga gcccttggtc ggagcaatcg tcatcagaac caccagtcca
    32401 agtgacagta attgtctgga attgctgtgc actcaatggt ttttgtagcc caaacatgaa
    32461 aagaagcagc aataggctgc aataattttt ccagttaggc gtttttgtta aaaatcgttt
    32521 tttcataggt taataattac gtggtttact aatcataata tataaattaa tcggttttat
    32581 tttttttatt ttgttaattg cttaacaaaa cacagtcgct aatttagcat ttttttataa
    32641 ttgtgtgcaa aataatgcta ttttttgtca acttctaaca aaaatatcca caataaagca
    32701 ttcgcttcca tttctaatgt ttgataatag ccaatactat tcattagttt gctatcattc
    32761 aagtctgttt caccaagcgc taatggagtt gaaatgtctt ttaagataaa gtatgctaat
    32821 ggttcgatat ttatgttttc agctggctga tacttattca tacgaagtac ctttaaagat
    32881 aaaaatttat ggtagttcca ttcctcttta tcttttacat acttattaaa taataaatta
    32941 gaaaaaaact gttctgtttt ttctaattta cttttttgct gagcgctcaa attatgagaa
    33001 ttgtatttct ttagaaaata aatattgttt aaagcatagt aaggtcctaa gaattcatca
    33061 acagtagctt gctcttcgag ttgctctaca atatcattgg gcaattttac ggattgtgga
    33121 tatatacaat aaaatagtaa ttttcttatt ggaagcaatt tggcttcttg aatacaatct
    33181 tctgtaatga ttgcattttc atttaagagt ttactataca aataaaagtc tttgtagtag
    33241 taatcattta aattagcaat ataacttttg gtttgaagat taaaagagtc taattgatgc
    33301 gtacgtttta gataagctaa ttcagcaaag gtttcttgtt tgatttttaa ttgatctaag
    33361 aatgaagttt ttctgtataa aacttcagca gccaatttat attgctcttt agtcaattga
    33421 gctttacaag tagtagagag aaaaagtata agtgctaagt gtaaaaaatt tttcatagaa
    33481 tagttctcgc aaatatgtga gaaatatttt taataaaaaa atttttttta cattttattt
    33541 ataatcgtct tctaacagat ttttcaattc ttttcgcgca aagaatattc tgcttttaat
    33601 agtacctaat ggtagattta attgttctgc aatttcatga tatttataac ctttgtagtg
    33661 cattaaaaaa ggcactctaa gttcatttga aattttcatc aaagcgttgc gaatatcatt
    33721 cattacaaat gctctttctc ctgcattgct aattgttgat gaggtgttta tataatatag
    33781 attcgctgtg gtatcaatga ctgtatttcg ctttgatttt tttctataat cattaataaa
    33841 aatatttttc atgatggtga ataaccaagc tttaatattg gtgccttctt gaaatttatc
    33901 ttcattaact aatgcacgta ctatggtttc ctgaatcaaa tcttctgcat catagatatt
    33961 acttgtcaga cgcattgcat gtggctttaa taccgttgtc gctagtttca cttctgattt
    34021 aaaattaata tcattcatat tgtatatttt aatctttaca aaattatgtc attaaggttt
    34081 tacttagtgt taattttgtc taaataagaa ctattgatgt gcatatagat tttttctatc
    34141 ataaaaaata agactacaaa actctttcta tcaaaatatg taaccattaa atagaaagcc
    34201 taaaatatta ctgttgtaga aaataacata agtaaagaaa attgtgctca caattaaaag
    34261 aaaaaagatt tcactacatt taaaaaaaat aaactaatga aatcacttta ttttacagaa
    34321 gaacatgagt tattcagaca gagcgttcgc caatttgtgc aaaaagaaat attgccttat
    34381 ggcgatcaat gggaaaaaga tgaaaaaata ccacgcgatt tatttttaaa actaggcgaa
    34441 caaggatttt taggcatcaa ccacgaagag gcttacggcg gttcaaaagc agatattttt
    34501 tatacttgtg cctatctcga agaattagca aaatgtggct atgctggtgt atgtgctgct
    34561 gtaagtgtgc atcaatatat ggcaacaaat catttagcgg aagctggcag ccacgaaata
    34621 aaagaaaaat ttttaagacc atctatcgaa ggtaaaaaag taggcgcgat tgctgttact
    34681 gaacctttcg gtggctccga tgttcaagga atgcgcacaa ctgcgcaaaa agatggtgag
    34741 cactatatta tcaatggttc aaaaacattt atcactaatg gtcatttctg tgacttcgtt
    34801 gttgtagcat gtaaaacaga tagcaatgct ggtataaatg gcattagtct tattatcgtt
    34861 gaacgcgaca cacctggttt tacttcatcg caattaaaaa aaatcggatg gcattcatca
    34921 gacactggcg aattagcatt tgacaacgtc aaagttcctg taagcaattt aatcggaaaa
    34981 gaaggaatgg gattttttta tataatggag agttttcaaa tcgaaagatt ggtggctggt
    35041 atcattggta ttggcggtgg agaccactgt ttggaagaaa cattaaaata catgaatgaa
    35101 cgagaagctt ttggacgacc aataaaaaaa ttccaagttt tacgccatga aattgtacag
    35161 ctatatactg aactcgaggc tggtaaacaa atgacatata atacttgctg gttaatgcaa
    35221 aacggagaag ttcctgtgaa agaagcatca atggtaaaat tatatatgac tgaacttgga
    35281 aacaaaattg tagataaatg tttgcaaatg tttggtggtt atggatacat ggaggatttt
    35341 ccaattgcac gtgcatatag agatgcgcgc gtaggtacaa ttgttggtgg aactacgcaa
    35401 attatgcgtg aaatattatc taaaataatc atcgacgata ttcgatataa gaaagtatat
    35461 agcaatgata gttcagtagc agcagcttca aattcaaata gcacaaacaa tgaaaaaagt
    35521 tggggaaatc caagtactgc aaaagaaatt attgcttcca ttccattgcg aataaaaaaa
    35581 gaaaaagctg ctgattattc aacaacattc caatttgata tcgctggtga aacaggcgga
    35641 caatacacat tgtttgtcaa caaaggtgat gcaaaaatag aagctggatt gaacggtact
    35701 cctgaatgca ttattacagc agatgccaaa gtttacgaag atattgaact aggcagaatg
    35761 gatcctacaa tggcatttat gggtggtcaa atcaaagtga cgaatattgg cgcaatgatg
    35821 caattcgcaa aatattttca cagaattcaa tagttagaat tcaattctaa tacaattcta
    35881 caataaaaaa aatctccggt ttatgccgga gatttttttg tgtttatcgt gatgatttt
//
LOCUS       DAOQQV010000016        31070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_421_length_31070_cov_6.951862, whole genome shotgun sequence.
ACCESSION   DAOQQV010000016 DAOQQV010000000
VERSION     DAOQQV010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 31070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31070
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_421_length_31070_cov_6.951862"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3767
                     /gene="porU"
                     /locus_tag="PLL59_03240"
     CDS             <1..3767
                     /gene="porU"
                     /locus_tag="PLL59_03240"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HMY22849.1"
                     /translation="AQQYIDESSIIELHWQQKDKLFSFDNAVLQSEYLGLPKFSKTIK
                     SKTKNDFTVEIVEPSYTPIAANSFSKEQIQLLSSQLQIQQSVGVDRKQFVTTISFLPY
                     TYKDGQVLQLQSFKLKTLPVLTSASREINISSRTYVSNSMLQNGKWFMFGVPNNGVYK
                     LDYSFLKKLGIAVDNIHPNNIRIFGHRGGMLPELAGADKDDDLREFPLKVVTSSPNVF
                     GSNDYVLVYLPGPESWTYNTTKQMFVAKKHTYSDTKNFFITTDAGVGSRMTAINGISL
                     PETNTVNTYNDYAFIEDDRTNLAHSGRVFVGDEFGGVTERSYAFSFPNLLSGQPVKLD
                     ISVVAAADVNGSYFNVSTDGAANANSIYIPRVDDFLGIKDVAERQERQLNLFNVPTNF
                     NVHLSFDRNGDFNTKGWLDYLQANAVSILRYNNQALYFRNISSVGIGNVSKFEIQNMN
                     ANVEIWDVTNPFATKKINYQLLNNIAVFTLATDSLREFVAIENTNNVPIAFGSIANQN
                     LHAMPQQDMLIVTRNAMLPQAQELAQFHRDKQGLNVAVVELNQIYNEFSSGTNDISAI
                     RDFVKMFYDRAAGDSALMPKYLLLFGDGTYNNKSLHDYLLPCYQSQASFESLQTYVSD
                     DYFGLMDDNEGANINNTAAEIMDVAVGRIPADDFTKAQTAVEKIKRYYVNAAYGDWRN
                     QGTFVADDEDRNIHIADANDIADSYAAQVKKINIDKIYLDAYQQQSSTGVASYPAVND
                     AINRKLFTGTLFLNYIGHGGALGLAKERILTAVDIDQWENQNKLPLFITATCEFAPYD
                     KVDENSAGERVLFKPNGGGIALVTTTRVVYSHTNKTMNTNFMTQMKNASVNSSVNLGD
                     IIREAKKYTNTADGNRKFTLLGDPAIRLAFPTYQVKATSINNQAFSSPHDTLKALSKI
                     TIEGEVQDNNNQLFNTFNGTTSIVIFDKSKTINTLVNDPVSINNPDGSLPYDFTLQKN
                     TIYKGKTKVTDGKFKFTFLVPKDIDYSYGFGKMSMYASADTTDASGYTTDIVVGGAYD
                     TLLTDNEGPDVDVFLNDDKFVFGGITDESPKLFAKLKDENGINTSGNSVGHDITAIID
                     NNTQRIYNLNDFYENELDDITQGKVTFPFSGLAKGRHTLEVKAWDVLNNSGIGYTEFI
                     VEEKANLALSHVLNYPNPFTTNTKFMFEHNKPGASLDLKIEIFSVSGKIIKTITKNIN
                     SAGYRVDDVSWDGLDDYGDKIGKGVYIYRVSLKDDSGKKVSQYQKLVVLN"
     gene            3897..5165
                     /gene="porV"
                     /locus_tag="PLL59_03245"
     CDS             3897..5165
                     /gene="porV"
                     /locus_tag="PLL59_03245"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HMY22850.1"
                     /translation="MRYILRPNILIAALLTVSVFFELKAQGNQTINSDLTRVVNTAVP
                     FLRIVPDARGGAMGDVGIATPVDPNATFYNASKLAFAKKRLGLSLSYAPWLRALGITD
                     IFMTHFTGYYKVSSNDVVHTSLKFFSLGDIDFTDAAGNPIGSGRPRELSFDAGYSRKL
                     GKHFALGVAFRYIYSNLASGQSIGSEPIKAGHAFGSDVTATYNLDMTVGKKKMKANFM
                     TGIAVTNMGTKIAYTANAREKDFIPANFGLGVGFKLQPNKYHEWGIYADFNKLLVPTP
                     KVGDKDSNNILDFKEQSSIKGMFTSLGDAPGGFKEEMREWMMGIGTEYWYDHIFAARM
                     GYFLEAKDKGNRRFFSAGVGVKYSVFGLDFSYLVPTNGARNPLDNTFRFTLKFDFAKL
                     GGNKTNDDGEDINDQTDKVDKSTRKKLKQK"
     gene            complement(5240..5896)
                     /locus_tag="PLL59_03250"
     CDS             complement(5240..5896)
                     /locus_tag="PLL59_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMY22851.1"
                     /translation="MDNAKIKTDIAAFCFNPFQENTYVIAHTNKDCWIIDPGCYTLQD
                     QKTLISYLEKNQLKPVKLLNTHCHLDHIYGNKFIADEFNLALGIHEKELPILQRADMA
                     ARMFGAKIPDKCEPGYFIQEGDILTLEDAEFQILFTPGHSPGSICFSNTEEEYVIVGD
                     VLFMGSIGRTDLPGGDYDTLIHSIRTQLLPLASATAVFNGHGPSTTIGEEARSNPFLR
                     "
     gene            6092..9478
                     /locus_tag="PLL59_03255"
     CDS             6092..9478
                     /locus_tag="PLL59_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22852.1"
                     /translation="MQFGQNRVQYHSFDWQYYNSDNFTIYFYPGGQEIGKFVWTTAEM
                     KLKELNKQMDFHYRSQVDILIYNDITDLAQTNIGLGQEDYNIGGTAVLRDNKLFLHYD
                     GNHRHLQRDLMKGLSALYIRQMMAGNSFGQKIQNALFLVMPEWYTEGLSAYIGEPWNT
                     DLDAQLKKYLLTTKKSDFNRLVNINPVLAGQSFWYMINEMYGKNAIPNILYVTRVNHN
                     INKGFVYSVNTSIETLMKDWKEFYLKRFVSDNTDRDSTTAYKAIKIKKRNNNLIGDIK
                     TSFDGKYIAYAAFDGGFYKVFVQDVLTKKTKQVSKGGFRSDQYPFDKNYPILTWTKSG
                     HKLAIISEKKDIIRLKQVDLDKHTTTKNNIIKFKRIYGADFTNNPNLMVVSGQNKGQT
                     DLYLYNTANTNATQLNNDIYDDLNPVSVNWNGNEAVLFSSNRTENKFYLEAIDTVLPT
                     HTLDLFYYDLSKRGTDLIQVTETPFANEKPIGNYNEKYLTFLSDENGISNQYVATIDS
                     FYVGKDSVELYINEFTDSSKLVKQEIDKYVWKGTSFPISNFSKGIEQASNSRYADFLF
                     FGANKRKSYVYTKPLLAEETLLSLKSLGLRATAFRQFYKENVLTASTFKAPNKKNTNA
                     SNIDVDIDDDISLDSLYNAATRYTFESDFNNVLQTDKSIVENNPVNTTKNATIADSSV
                     VGASNDEAPPFLQNSPVPVDRSVNMEKTTGQKIRILPYRARFTSDYVVTQLDNSIMTQ
                     NYQSFNQNLGSYNFPSLSGMINFGVSDLMEDHKIFGGFRIPFDFKGFETYVKYVNLKK
                     RIDKTILYYRRSDKINFNVQDVNGNTLPYIYTGKTRTNFFESAFSFPFDITKSLRWSV
                     SYRNESLDIALTDQVSLALKDVKENWVSGRLEFVHDNSKEIQFNIYNGFRYKVFAEYF
                     YNANEKKSHIANIGFDVRHYQKVYRNIIWANRFAGATSFGPKKILYFLGGVDTWLNPK
                     YADDIPIATDQNYGLQAPVMNMRGLPQNIRNGNNYLLWNSELRIPLFSFFAKKPLKSA
                     FLRDFQLLGFFDAGIAYNFANPFDQKNSQSTEVVTPQPNNNPVVVTVNYYRNPFVFGT
                     GGGFRTNLLGYFLRLDVGFGYDGLQFNKKPMWHFSLSKDF"
     gene            9561..10433
                     /locus_tag="PLL59_03260"
     CDS             9561..10433
                     /locus_tag="PLL59_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HMY22853.1"
                     /translation="MLYSMTGYGKSTFKLGAKSYVIEIKSLNSKSLDLNFKVAAELRG
                     KEIEIRKQIADQLIRGKVDIFFQEDKSTNGNTNLNFNLIESYYQQLQQFTTEKNIPLG
                     DILPAILRFQDINKTELTELNDADVEALLMGMDNALKEVNKFRQKEGAQLERDLQERI
                     NIIQQHKIALLPFEQQRMEKLRSRLLQDLKTNLQENIIDKNRFEEELIYYMEKMDINE
                     EKVRLDAHILYFFEILNEQNKEKGKKLGFVAQEIGREINTIGSKSNDANMQKIVVQMK
                     DELEKIKEQVLNIL"
     gene            10514..11083
                     /gene="gmk"
                     /locus_tag="PLL59_03265"
     CDS             10514..11083
                     /gene="gmk"
                     /locus_tag="PLL59_03265"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HMY22854.1"
                     /translation="MQKVIILSGPSGVGKNTLGDFLLQQFPELSYSVSATSRSIRKGE
                     QDGVDYHFMNNEEFEQKIKEDALLEWQEVYDGMYYGTMKSELDRINSLNKFPLLVVDV
                     FGAINVMKNLKFKPLSIFIQAPSLEILENRLRTRGTDSEEKIAKRLEKASVEMNESAN
                     FDLTIINDDLPMAQKQLEEVVHYFLKFGF"
     gene            11174..11575
                     /gene="apaG"
                     /locus_tag="PLL59_03270"
     CDS             11174..11575
                     /gene="apaG"
                     /locus_tag="PLL59_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HMY22855.1"
                     /translation="MYNKKMVTAITHKIKVSVETFYQSDYSYPMSQHFMFAYRITIEN
                     NNNNTIQLLRRHWYIVDALGQQREVEGEGVIGEQPIIKSGERYEYISGCDLKTEIGKM
                     YGTYLMENLHDGKCFHVVIPEFYMVAPMKYN"
     gene            complement(11580..12809)
                     /locus_tag="PLL59_03275"
     CDS             complement(11580..12809)
                     /locus_tag="PLL59_03275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HMY22856.1"
                     /translation="MQEIKVHPPILNTITLCLKAIFEANQVADKVVANTLQENKNFGA
                     RDRSTIAETVYDIVRWKLKYEYQLAQLGEQFTYYKHLVFVSFVNRNYTIKNIELFDIT
                     NSEVLRLNEIIQKAIDEKYIEQSYPKTFYDFCLDSIGASWHQIAKALNVKPSVYIRVN
                     SLKTSAANLIEILLKEGIEIEAQAKNSYLKILSKNNLKNSLYYKQGLFEFQDIGSQEI
                     GNFLFHAIEDKSKIDNISILDLCAGAGGKSLHLSALLNNRGKIYATDYHAARLKNLQL
                     RATQAGCKNIEIIDFKTVKNLKNIDILLIDAPCSGSGTFKRQADLKYKISVEKINEYT
                     QIQENLLATYISTLKKNGKIVYATCSILPQENQLQIQNFLAKNIAQWQEKQSQQLLPT
                     MYDGDGFFMTLLERKDK"
     gene            complement(12816..13274)
                     /locus_tag="PLL59_03280"
     CDS             complement(12816..13274)
                     /locus_tag="PLL59_03280"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMY22857.1"
                     /translation="MISAKELIQRFGLEPHPEGGYYKRTHFDEHGSFSSIIFLLVKNN
                     FSAFHRIQSDEQWNWYYGDAIIIHEIEKNGDYHQHKLSNHLQQAHFQHIVRKGNWFAS
                     ECIGEYGFALCGCTVIPAFSFEKFELAKCNELVANFPRHHEIIKRLTRKS"
     gene            13297..14382
                     /gene="proB"
                     /locus_tag="PLL59_03285"
     CDS             13297..14382
                     /gene="proB"
                     /locus_tag="PLL59_03285"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HMY22858.1"
                     /translation="MIFNSKISITLDKLSINMQKPILVFKIGTSSITDASGNLDEEVV
                     QNIAKQLATLHKEFQIVIVSSGAVGSGKKFIKNYSGKIEERKAAAAIGNPLLIQKYSA
                     AFSKYKIPIAQSLCERGHFSDRAKFLQLRATYEELWKNGIIPIANENDVVSSRELKFS
                     DNDELATLLAVGFGAQLLLIGTSVKGVLDGEGNLIKRIEKFDDKVMSLATKETSAAGL
                     GGMISKLTFAHMASSLGIKVHIFSAKEKNSLLNAVKEKTGTICIAKNVSKSARHKWLA
                     SGSLVIGSITVDEGAQKALLKRKSLLAVGVTAINTDFEIGEAIEIMDVNANVFAVART
                     KISSKILQKNIKQQNLILAHADDIVLI"
     gene            complement(14385..15383)
                     /locus_tag="PLL59_03290"
     CDS             complement(14385..15383)
                     /locus_tag="PLL59_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMY22859.1"
                     /translation="MLKRATKIIVFLLLILSSAIFLKFIFLGKKLDIATITPYLTKDK
                     LVHDSLSLTYLGCAGFIIENKEKSILCDPFISNPNIFNFSKKFDSWSERISPEKLKNI
                     DMVAISHGHYDHCYDITDLCNYLPSQAQIIADASVHNQLNVIYKAATFRKTALHYTDK
                     QQWIYENDSAFRIFALKSVHSPHIGKIEFFKGHYDTPLAKIPTKPWQWKKGEAYSYLI
                     DILENQRVKYRIVLVNGNLTIDAMQTLKTLSNERKSDIQLQIFWKEKLNIDNLLEVYN
                     ITQPKEIILHHWNNFFVSFDKPLQYMRDAHLPEVLKRLNEQEIPTSMMLPFTEVKL"
     gene            15450..16697
                     /locus_tag="PLL59_03295"
     CDS             15450..16697
                     /locus_tag="PLL59_03295"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HMY22860.1"
                     /translation="MESIQYLLEATNKAAVEVGKLSIVSKHAILKDLAAALLSQTEII
                     IAENAKDLQRMEDNNPKKDRLLLNAQRIKDLSDSLYDILKLEDPSGKVLSDNTMSNGL
                     NIQKIAVPIGVVGVIYEARPNVTIDVAALCLYAGNGVVLRGGSDAEFTNEILVKIIHS
                     VLEKNGVSKDAVSLLPVDRKFVKELLAAVKWVDIIIPRGSQQLIDFVRENSKVPTIET
                     GAGVCHTYIERTADLDMAAQIVVNAKTQRPSVCNSLDTVLVDKDIAKEFLPKLIDGFN
                     AFDVEIFADETAYNIFKDSNYTHLQPANADSFGMEYLSQKCSIKAVESFDDALAHIRK
                     YSSRHSEAIVSKDETLCERFLNEVDAAAVYTNASTRFTDGGCFGLGAEIGISTQKLHA
                     RGPFALEKLTTEKWKVRGSGQTR"
     gene            complement(16698..17573)
                     /locus_tag="PLL59_03300"
     CDS             complement(16698..17573)
                     /locus_tag="PLL59_03300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMY22861.1"
                     /translation="MSIKSKILDFVRIIVCRIPFIEKLILEQLRQGNVIAQKLLAGNH
                     LYPKNSIRHCTRNNINYELDISDYMQHAIYFHLTQHVDFDRRKLYSLIEANFVCIDIG
                     ANIGETTLNFAQIAKNGKVYSFEPVPFLFEQLIKNISLNDYNNIAAFNIAIADNEEDL
                     FFEMPSNKNSAGISLNKQQAGTAKRVVGTTLDNFIRQQNIPHIDFIKIDVEGFEYFVL
                     KGATQTILQHRPILFIEIDNSYLSAKNTSEKEVLSYLQNKLGYTLYRINGIAQEKINN
                     LENTNLHYDVLCVFE"
     gene            complement(17599..18252)
                     /locus_tag="PLL59_03305"
     CDS             complement(17599..18252)
                     /locus_tag="PLL59_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HMY22862.1"
                     /translation="MIQLFSFFTFFLSLFGLQSGNQLNDPKAKTILDAVSKTYKSYNA
                     IEAGFSLTTNNPGQNKSTVQNGNVFLKGEKYKLSLGKQIIYCDKQSVWTYLKDINEVQ
                     INDYEPNKEDITPSNMFTIYQNDFNYMMNGEEAVDKTSCTVIDLMPKDKSKPFFKVRI
                     WIDKNAKYIKRMKVFDKNGTLYTYSVTSFNSKANLEDSFFKFDAAKHPGVHVEDLRM"
     gene            complement(18328..20913)
                     /locus_tag="PLL59_03310"
     CDS             complement(18328..20913)
                     /locus_tag="PLL59_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HMY22863.1"
                     /translation="MAEEVVKEKVKRTKKAPINEAVEESEPAPLIETDTLKTGFGLFF
                     LFLAITLAVAFGSYLFTWKDDQDKVLNFSWKIIFSKEALVENRMGRIGAYLAHVFFYV
                     FFGVASFIIPYLLFCIGINLTFKERIFKLTKIFFSSLFWIVFIATLASFIFSNYDFPF
                     GGGLGRNNYEWLSTLIGNLGLGILLGIIGFSFLGFNLKDIYEKFTRWKQTWKAQYEAE
                     NKNSSTAEIDDFYKEEALKKEDGYDMESIIPEEKEQAIPHHKDETFEILNEQEEVIQS
                     NSKEIKMELIEKETEEIEFENTTIEPTSKKEKFSLEPDFEIEEIQAFGTIDENANAAW
                     THIQKENETDFEADKEHFGLDTNFDPKLELSKYKFPNVDLLEEHGSGQIEINKEELEQ
                     NKNQIVQTLSHYNIGIEKIKATVGPTVTLYEIVPDKGVRISKIKNLEDDIALSLSALG
                     IRIIAPIPGRGTIGIEVPNEKKSIVSMRSLIAGEKFQKSDMDLPIALGKTISNEDFVV
                     DLAKMPHLLMAGATGQGKSVGLNAILVSLLYKKHPAELKFVLVDPKKVELNLYKLIEN
                     HYLAKLPDEEEAIITDNKKVIYTLNALCIEMDTRYELLKAAQVRHIKEYNQKFLKRQL
                     NPNNGHRFLPYIVLVVDEFADLMMTAGKEVEAPIARLAQLARAIGIHLVIATQRPSVN
                     IITGTIKANFPGRIAFKVTAKVDSRTILDASGADQLIGRGDMLLSQSGELTRLQCPFV
                     DTPEVEHIAKFIGTQKGFPTPYELPEYIDPKEADKNGERSFDFSQRDELFTEAARLIV
                     RNQIGSTSLIQRKLALGYARSGRLMDQLEAASIVGPNLGSKAREVYVKTDEELERILQ
                     ELGEQ"
     gene            complement(20995..21234)
                     /locus_tag="PLL59_03315"
     CDS             complement(20995..21234)
                     /locus_tag="PLL59_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HMY22864.1"
                     /translation="MITLIKKYKLTLIGVVLGAMAGYAYYYYVGCLSGTCAITSNPWN
                     SILYGALMGGLFFNIFQEKKEEMKAFENKETHSDK"
     gene            complement(21244..22668)
                     /locus_tag="PLL59_03320"
     CDS             complement(21244..22668)
                     /locus_tag="PLL59_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMY22865.1"
                     /translation="MKIEQIYTGCLAQGAYFIESNGEVAIIDPLREVQPYIGMATKRN
                     AKIKYIFETHFHADFVSGHVDLAKETGATIIYGPTAMETGYAVHKTHDGEVFTLGNAT
                     ITVLHTPGHTMESICLLLTDSNGKKTALFTGDTLFIGDVGRPDLAQHVVSDLTQEKLA
                     AHLFDSLRNKIMPLPDDLIVYPAHGAGSACGKNMSKETSDTLGNQKKTNYALNPSLTK
                     EEFIKQVLDGLVAPPSYFPKNVLLNITGYDSIEKVIDRGTRALTPDEFEAIANETNAL
                     LLDTRSPSEFNKAFIPNSINIGIDGSFAVWVGTLVSDIKQAILLITEPGREEEVITRL
                     SRVGYDNTIGYLDGGIAAWQRVAKETDSITSITADELAQLQSENPALSILDVRKKSEY
                     FSEHIQEAENLPLDFINDTMQQIEKNKTYYVHCAGGYRSMIFTSILRARGYHNLIDVA
                     GGFAAIKKSEKFELTDYVCPTTML"
     gene            22841..24340
                     /gene="glpK"
                     /locus_tag="PLL59_03325"
     CDS             22841..24340
                     /gene="glpK"
                     /locus_tag="PLL59_03325"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HMY22866.1"
                     /translation="MKMQKKYILALDQGTTSSRAILFDANAAIVTVAQKEFTQIFPQP
                     GWVEHDPIEIWQSQIEVAREAVAKANIQPTEIAAIGITNQRETTIVWDKETGKPIYNA
                     IVWQDRRTAGFCDQLKARNLESYVRENTGLVIDAYFSGTKINWILDNVEGARQKAHEG
                     RLCFGTVDTWLIWNLTKGKVHATDYSNASRTLIYNIKDLKWDEQLLHDLDVPKSILPE
                     VLPSSGIFGKTHADVFFDIEIPIAGVAGDQHSALFGQTCFEAGMAKNTYGTGCFMLMN
                     TGTERVLSKHGLLTTIAWSIGGKVEYALEGSVFIAGAAIQWLRDGLKLIDTAPDSEYY
                     AMKVEDTGGVYVVPAFAGLGAPYWDMYARGGIFGLTRGTQKAHLIRATLESLAFQTYD
                     ILEVMEKDADIQLKSLKVDGGACSNNLLMQFQADILEVNVERPKVIETTALGAAYLAG
                     IAVGFYKKENLANNEHIHATFSPKMKSEKREKLIKGWKKAVERSKNWED"
     gene            24437..24631
                     /locus_tag="PLL59_03330"
     CDS             24437..24631
                     /locus_tag="PLL59_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22867.1"
                     /translation="MKKLFVLFAAATLFVGVQACKQKEEAATEEATEVTTEEVVTDSA
                     AVAAPVDSAAAAPADTAATK"
     gene            24770..25048
                     /locus_tag="PLL59_03335"
     CDS             24770..25048
                     /locus_tag="PLL59_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22868.1"
                     /translation="MKKLFVLFAAATLFVGMQACKQTAEGAAEDAAAATDAVTETATD
                     AAATVTETAGAVADSATAAAGAAVDAAAGAAKDAAGAAVDKAAEAVKK"
     gene            complement(25119..25370)
                     /locus_tag="PLL59_03340"
     CDS             complement(25119..25370)
                     /locus_tag="PLL59_03340"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HMY22869.1"
                     /translation="MKKQDITVIGAAFFAVLYLINPTAGFIELIPDNIPLIGNLDEAG
                     AVFILIAALKYFGFEMPSIFKKDNEKKDSDTKPTIIINK"
     gene            25518..25591
                     /locus_tag="PLL59_03345"
     tRNA            25518..25591
                     /locus_tag="PLL59_03345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25552..25554,aa:Met,seq:cat)
     gene            25683..25988
                     /locus_tag="PLL59_03350"
     CDS             25683..25988
                     /locus_tag="PLL59_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HMY22870.1"
                     /translation="MSAYAFHPDSATQEDVDVLEKELLEYSHQLIVYNDDVNTFDWVI
                     ESLMAICKHTHEQATQCALFIHFRGKYAVQHGDELTLIPMKDALLDRGISAAVESCN"
     gene            25979..26668
                     /gene="aat"
                     /locus_tag="PLL59_03355"
     CDS             25979..26668
                     /gene="aat"
                     /locus_tag="PLL59_03355"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HMY22871.1"
                     /translation="MQLTVLPIYLLTEKLVFPPVDGAENGIVAIGGDLSTERLLLAYR
                     NGIFPWYSDEEPIVWHAPDPRFVLMIEDLHVSKSMRRVLNSKKFKVSFDTNFQFVIQQ
                     CATVERVGQDGTWITNAMQNAFINLHKEGFAHSVEVWNQDRIVGGLYGVCLGKVFFAE
                     SMFHTESNASKVALICLVNFLKQIEVQFIDAQVYTSHVESLGAKNISRNAFMELLKSA
                     LNFDTFRGKWI"
     gene            26864..28456
                     /gene="aceB"
                     /locus_tag="PLL59_03360"
     CDS             26864..28456
                     /gene="aceB"
                     /locus_tag="PLL59_03360"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HMY22872.1"
                     /translation="MSTYQTPTGIEINGKYTSEFAEILTPDALAFFAKLQRKHNARRK
                     ELLQKRVDRQVRLDAGELPDFLAETKAIREGAWTVAPCPADIQDRRVEITGPVDRKMI
                     INALNSGAKVFMADFEDSNAPTWENNISGQINLKDANNKTISLESNGKSYKLNDTIAA
                     LFVRPRGWHLLEKHVLVDGEVASGSIWDFALYFYHNAKQRVEKGSGVYFYLPKMESHL
                     EARLWNDIFVDAQNELGIPQGSIKATVLIETIVATFELNEILFELKEHSAGLNCGRWD
                     YIFSFIKRTVKNKQFLVPDRMQVTMTTHFLKSYSDLVIQTCHKREAFAMGGMAAQIPI
                     KNDEEANNTALAKVRADKEREAKAGHDGTWVAHPGLVQIAMDEFNKYMPTPNQIHVKR
                     EDVNVSAADLLQVPVGTITLGGLKNNIDVGIQYLEAWLRGNGCVPIHNLMEDAATAEI
                     SRTQVWQWVHHEDAKLDDGTKVTAELVQQLIDEQKAAYQTTLGDKYVASKFDLATELF
                     TKMSLQDTLEEFLTLEAYNYID"
     gene            28504..29787
                     /gene="aceA"
                     /locus_tag="PLL59_03365"
     CDS             28504..29787
                     /gene="aceA"
                     /locus_tag="PLL59_03365"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HMY22873.1"
                     /translation="MATKQERAETLKKDWATNPRWKDVKRDYTAEEVINVSGSVPVEY
                     TLAKNGAEKLWNKLQSEDWVAGLGAYNGNQAIQEVTAGMKAIYLSGWQVAADANLSAE
                     MYPDQSLYPANSVPMVVKRINNSLMRRDQIEYMSGEPTRDWMVPIVADAEAGFGGNLN
                     AFELMKQMIEAGAAGVHWEDQLASAKKCGHLGGKVLVPTQEAINKLSAARLAADVCGT
                     STLVIARTDAEAANLITSDIDPRDHKFIIEGSRAPEGFYYVKNGLEQGIDRGLSYAPY
                     ADLIWMETGNPDLGMAREFAQGIHAQFPGKMLAYNCSPSFNWAKYMDVKAMTTFREDL
                     AAMGYKFQFITLAGFHANNTVMFELSKAYMERGMAGYSELQQREFALESAGFQAIKHQ
                     SFVGTGYFDAVQQICQQGQSSTTALAGSTEAEQFH"
     gene            complement(29872..30741)
                     /locus_tag="PLL59_03370"
     CDS             complement(29872..30741)
                     /locus_tag="PLL59_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HMY22874.1"
                     /translation="MQIEASELIVNPDGSIYHLNLQADDIADTIFLVGDPDRVSEVSC
                     YFDKIEVKKQKREFITHTGFIGSKRLTCISTGIGTDNIDIVLNELDALVNIDLKTRTI
                     KPNLTALNLIRIGTCGSLQADIEVDSFLASEYGIGLDGLLGFYNFDISESDKQFIEPI
                     IPIVRSLGVHPSISKASEKLLETIGKDMRKGVTLTNCGFYGPQGRTVRIAPKDKHYID
                     ELSAINLPGNRKFTNMEMETSAIYGLGQLLGHHALSCNAILANRVLGTFSADPHKATD
                     NLIRHVLEKISVN"
     gene            30872..>31070
                     /locus_tag="PLL59_03375"
     CDS             30872..>31070
                     /locus_tag="PLL59_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMY22875.1"
                     /translation="MITPHQILKEYFGFVDFRQAQLEIIQSVLAKKDTLALLPTGGGK
                     SICFQVPALMQDGICIVVTPLI"
BASE COUNT        10251 a         5116 c         5578 g        10125 t
ORIGIN      
        1 aagcgcaaca atatattgat gaatcttcaa ttattgagtt gcattggcag caaaaggata
       61 agttatttag ctttgataat gctgtattac aatcagaata tttaggctta cctaaattct
      121 caaaaacaat aaaatcgaaa actaaaaatg atttcacggt agagattgtc gaacccagtt
      181 atacgccaat tgcggcgaat tcattttcta aagaacaaat acaactactg tcttctcaat
      241 tgcaaatcca acaatcagtt ggtgtagata gaaaacaatt tgttactaca atttcttttt
      301 taccttatac atacaaagat ggacaggttt tacagttgca atcttttaag ctgaaaacat
      361 tgccagtatt gacatcagca tcaagagaga taaatatttc aagtcgaacc tatgtgtcca
      421 attctatgtt gcaaaatggc aaatggttta tgtttggtgt gcccaataat ggcgtatata
      481 aattagacta ttcatttctt aaaaagcttg gcattgctgt tgataatatc catccaaata
      541 atattagaat ttttggtcat cgtggcggta tgttgcctga attagcaggc gcagataaag
      601 atgatgatct tagagaattc ccactaaaag tagttacatc gtcaccaaat gtatttggaa
      661 gtaatgacta tgttttggtc tatttgcctg ggccagaatc ttggacttat aatacaacaa
      721 aacaaatgtt tgttgctaaa aaacatacgt atagcgatac taaaaatttc tttattacaa
      781 cagatgccgg agttggaagt agaatgaccg caataaatgg catttcgctg ccagaaacaa
      841 atactgtaaa tacctacaat gattatgcat ttattgaaga tgacagaact aacctagcac
      901 attctggccg cgtgtttgtt ggcgatgaat ttggtggtgt gacagagcgg tcttatgctt
      961 tttcatttcc taatttatta agtggacagc ctgtgaaatt agacatttcc gtagttgcag
     1021 ctgctgatgt gaatggttct tattttaatg tttcaacaga tggtgctgca aatgccaaca
     1081 gtatttatat tccgagagtt gatgattttt taggtattaa agatgttgct gaacgacaag
     1141 aacgacagtt gaatttattt aatgtgccta ctaattttaa tgttcatttg tcatttgata
     1201 gaaatggcga ttttaataca aaaggctggt tggattattt acaagcaaat gctgtttcaa
     1261 tattacgtta taacaatcaa gcgctgtatt ttagaaatat ttcttctgta ggtattggca
     1321 atgtttctaa atttgaaatc caaaacatga atgcaaatgt tgaaatttgg gatgtcacaa
     1381 atccgtttgc aacgaaaaaa attaattacc aattattaaa taatattgca gtgtttactt
     1441 tggctaccga ttctctacgt gagtttgtag cgattgaaaa tactaataat gtaccaattg
     1501 cttttggaag tatagctaat caaaatttac atgcaatgcc acaacaagat atgctaattg
     1561 ttactcgtaa tgccatgttg ccgcaagcac aagaactggc acagtttcat cgtgataaac
     1621 aaggcttgaa tgttgcagtt gttgaattaa atcagatata taatgagttt tcttctggca
     1681 caaatgacat aagtgccata cgtgattttg taaaaatgtt ttacgataga gcggcaggcg
     1741 attctgcgct gatgcctaag tatttattgc tatttggcga tggtacttat aataataaat
     1801 ctcttcacga ttacttgttg ccgtgctatc aaagtcaagc ttccttcgag tctttacaaa
     1861 cgtatgtgtc cgatgattat tttggtttaa tggatgataa tgaaggtgcc aatatcaata
     1921 atacagcagc tgaaattatg gatgttgctg taggacgaat tccagctgat gacttcacaa
     1981 aagcacaaac agcagtagaa aaaattaaac gctattatgt aaatgcagct tatggcgatt
     2041 ggcgtaatca aggcactttt gtcgctgatg acgaagacag aaatattcat atcgccgatg
     2101 caaatgatat agctgacagc tacgctgcac aagtaaaaaa aataaatatc gataaaatat
     2161 atttagatgc ttatcaacag caatcaagca ctggagtagc aagctatcca gccgttaacg
     2221 acgctatcaa ccgtaaatta tttactggaa ctttgttttt aaattatatt ggtcacggcg
     2281 gcgcattagg attagccaaa gaaagaattt taactgcagt agatatcgac caatgggaaa
     2341 accaaaataa attgccatta tttattactg caacatgcga gtttgcacct tatgataaag
     2401 tagatgaaaa ttctgctggt gaaagagtat tgtttaagcc aaatggtgga ggcattgcat
     2461 tagttacgac aacacgtgtc gtgtattcac acactaataa aacgatgaac actaatttta
     2521 tgacacaaat gaaaaatgca agtgtaaata gtagtgtgaa tttgggtgat atcattagag
     2581 aagcaaaaaa atatacgaat actgctgatg gaaatagaaa gtttacttta ttaggcgatc
     2641 cagctattcg attggctttt ccaacttatc aagtgaaagc aacatcgata aacaatcaag
     2701 cattctcatc gccacatgat acattaaaag ctttgtcgaa aattacgatt gaaggtgaag
     2761 tacaagataa taataatcag cttttcaata cctttaatgg tacgacatct atcgttattt
     2821 ttgataagtc aaaaacgata aacacattag tgaatgatcc agtaagtatt aataatccag
     2881 atggtagctt gccgtatgat tttacattgc aaaaaaatac tatttataaa gggaaaacaa
     2941 aagtgacaga tggtaaattt aagttcacct ttttagttcc taaggatatc gattattctt
     3001 atggttttgg taaaatgagt atgtatgcat cagccgatac aacagatgca tcaggttata
     3061 ctactgatat tgtagtagga ggcgcttatg atacgctgct cacggataat gaaggtccag
     3121 atgttgatgt gtttttaaat gatgataaat ttgtattcgg tggaattaca gacgaatcgc
     3181 caaaactatt tgcaaaattg aaagatgaaa atggtataaa cacatcaggc aatagtgttg
     3241 gacacgatat tacagctata atcgataata atacacaacg catttataac ttaaatgatt
     3301 tttatgaaaa tgaattggat gatattacac aaggaaaagt aacttttcct ttctccggtt
     3361 tagcaaaagg aagacatact ttagaagtaa aagcatggga cgttttaaac aattcaggta
     3421 ttggttatac agaatttatt gtagaagaaa aagcaaattt agccttgtcg catgtgctca
     3481 attatccaaa tccttttaca acaaacacaa agtttatgtt tgaacataat aaaccaggcg
     3541 catcgctaga tttgaaaata gaaatcttct cagtttcagg caaaattatt aaaacaatta
     3601 ctaaaaatat taacagtgca ggttatagag ttgatgatgt ttcttgggac ggattagatg
     3661 attatggcga taaaattggg aaaggtgttt atatctatcg tgtgtcctta aaagatgata
     3721 gtggaaaaaa agtgagccaa taccaaaaat tagtggtgct aaattagaat ttttcaaata
     3781 aatttaccat tttaacgttt tgaattctga aatactaaac aacaattgtt aataattctc
     3841 ttatattgca tggcaatagt gaatactgac aaaattattg tataataaaa tcgtacatga
     3901 gatatatttt aaggcccaac atactgatag ctgcattact tactgtgagt gttttttttg
     3961 aattaaaagc acaaggaaat caaacgatta acagtgattt gactcgagtt gtcaatactg
     4021 ccgttccttt tttacgcatt gtgccagatg cacgtggtgg cgcgatgggc gatgtcggta
     4081 ttgccacacc agtagatcct aatgctacat tttacaatgc ctcaaaattg gcatttgcta
     4141 aaaaacgttt agggctttca ctgtcatatg cgccttggct acgtgcttta ggtattacag
     4201 atatttttat gacacatttt acgggttact acaaagtatc aagtaatgat gtagtacaca
     4261 cttcattaaa gttttttagc ttaggtgata ttgacttcac agatgctgcc ggaaatccga
     4321 taggaagcgg tcgtccacgt gagctttcgt ttgatgcagg ttattctcgc aaattaggca
     4381 aacattttgc ccttggcgtt gcttttaggt atatctattc aaatttagca tctggtcaaa
     4441 gcattggtag cgaaccaata aaagctggtc atgccttcgg ttctgatgtc actgctactt
     4501 ataatttaga tatgacagtc ggcaaaaaga aaatgaaagc caacttcatg acaggcattg
     4561 cggtcactaa tatgggtaca aaaattgctt acacagcaaa tgcacgagaa aaagatttta
     4621 ttccagcaaa cttcggatta ggtgttggtt tcaaattgca acctaataaa taccacgaat
     4681 ggggaattta tgccgatttc aataaattat tagtgccgac gccaaaagta ggcgacaaag
     4741 acagtaataa tatattggat tttaaagaac aatcttccat aaaaggaatg ttcacctcct
     4801 taggtgatgc acctggtggc tttaaagaag aaatgagaga atggatgatg ggcatcggta
     4861 cagaatattg gtatgaccat atttttgctg ctcgtatggg ttatttctta gaagccaaag
     4921 ataaaggtaa tagacgcttt ttctctgctg gtgtaggtgt aaaatatagt gtatttggtc
     4981 tagacttttc ttatctagtt cctacaaatg gcgcaagaaa tccattggat aatactttcc
     5041 gttttacctt gaaatttgac tttgctaaat taggtggtaa taaaacgaat gatgatggcg
     5101 aagacatcaa tgaccaaacg gataaagtag ataaatctac acgtaaaaaa ttgaaacaaa
     5161 agtaagcaat actttatatg ttataaaaag cggctgactt ggaacaagtc agccgctttt
     5221 ttagttgtca ctaatttaat taacgcagaa atggattgct ccttgcttct tcgccaatcg
     5281 tggtacttgg tccatgtcca ttaaacacag cagttgctga tgctaatggg agcaattgtg
     5341 tgcgaatgga atgaattaat gtatcgtaat caccgccagg caaatctgtt cggccaatgc
     5401 ttcccataaa cagcacatca ccaactataa catattcctc ttctgtattg gaaaaacaaa
     5461 tacttccagg tgaatgtcca ggtgtgaaaa gtatttggaa ttcagcatct tctaatgtca
     5521 aaatatcacc ttcttgtatg aaatatccag gctcacactt gtcaggtatt tttgcaccaa
     5581 acatacgcgc tgccatgtcg gctctttgca atatgggcaa ttctttttca tgaatgccta
     5641 atgctaaatt aaattcgtca gcaataaact tattgccata tatatggtct aaatgacaat
     5701 gtgtattaag cagttttact ggcttaagtt gatttttttc taaatagctt atcaatgttt
     5761 tttggtcttg taaggtataa cagccgggat cgattatcca acaatcttta ttagtatgag
     5821 caataacata cgtgttttct tgaaaagggt tgaaacaaaa tgcagcaata tctgtcttta
     5881 tttttgcgtt atccataaga tttatttact ttgtaaaagt agtaaattca aataagtata
     5941 gcgaatacag tttggatatt aaaatatgaa tataaattta caaatacaaa gtgccaatac
     6001 ttatacaaag tgttttacaa ttgcaaagtc aattttattt ttttcatgct tgacttttta
     6061 ttctacttta cttttttcgc agactacaca agtgcagttc ggtcaaaatc gtgtacaata
     6121 ccattccttc gattggcaat attataattc agacaatttt accatttatt tttatccagg
     6181 tgggcaagaa ataggaaaat ttgtgtggac aacagcggaa atgaaactca aagagctgaa
     6241 caaacaaatg gattttcatt atcgctcaca agttgatatt ttaatctata acgacattac
     6301 tgatttggca caaaccaata taggcttagg tcaagaagat tataatattg gcggcacagc
     6361 agtacttcgt gataataaac tatttttaca ttatgatggc aatcatcgtc atctgcaacg
     6421 agatttaatg aaaggtctgt ctgccttata catcagacaa atgatggcag gcaatagctt
     6481 tggacaaaaa atacagaatg cactatttct agtaatgcca gaatggtaca cagaaggcct
     6541 gtcggcatac ataggtgaac cttggaacac agacttagac gcacaactaa aaaaatattt
     6601 attaactacc aaaaaatctg attttaatag actcgtaaat attaatccag tcttggctgg
     6661 acaatctttt tggtatatga taaacgaaat gtatgggaaa aatgccattc caaatatatt
     6721 atatgttacc agagttaatc ataatatcaa taaaggattt gtgtattccg taaatacatc
     6781 aatagaaaca ttgatgaaag attggaaaga attttatcta aaacgtttcg tgtctgataa
     6841 tacagataga gatagtacga cagcatataa ggctataaaa atcaagaaac gaaacaacaa
     6901 tttaattggt gatataaaaa cttccttcga tgggaaatat attgcctatg ctgcatttga
     6961 tggcggtttt tataaagtgt ttgtgcaaga cgtattaaca aaaaaaacaa aacaagtttc
     7021 aaaaggtggt tttcgctctg atcaatatcc atttgacaag aattatccta ttttaacttg
     7081 gacaaaaagt ggacataagc ttgctattat ttctgagaaa aaagatatta ttcgtttaaa
     7141 acaagttgac ttagacaaac atactactac aaaaaataat atcatcaaat tcaaaagaat
     7201 ttatggtgct gattttacca ataatccaaa tttgatggtt gtttctggtc aaaataaagg
     7261 tcaaaccgat ttgtatttat acaacaccgc taacaccaat gctactcaat taaacaatga
     7321 tatttacgat gatttgaatc cagtttcagt aaattggaat ggcaacgaag ccgtattatt
     7381 ttcttccaat cgtaccgaaa ataaatttta tctcgaagca atagataccg tattgccgac
     7441 gcatacttta gatttatttt attacgattt atccaagcgt ggaaccgact taattcaagt
     7501 aacagaaaca ccatttgcca acgaaaaacc aataggcaat tacaatgaaa aatatcttac
     7561 ttttttaagc gacgagaatg gtatttctaa tcaatatgtg gctacaatag atagctttta
     7621 tgtaggtaaa gattctgtag aattgtatat caatgaattc actgattcct caaaattagt
     7681 aaaacaagaa attgataaat atgtttggaa aggcacatca tttccaattt ctaatttctc
     7741 aaaaggaatt gaacaagcta gtaattcacg ctatgctgat tttttattct tcggtgcaaa
     7801 taaaagaaaa tcttatgtct atactaagcc tttgctggca gaagaaacat tattgtcact
     7861 gaaatcatta ggtttgcgcg caactgcatt ccgccaattt tataaagaga atgttttaac
     7921 agcgtccaca tttaaagcac ccaataaaaa aaatacgaat gcatcaaata tagacgttga
     7981 catagatgat gacatttctt tagatagttt gtataatgca gctacgagat atactttcga
     8041 atctgatttt aataatgttt tacaaacaga taaaagcatt gtagaaaata atccagtaaa
     8101 cacaacgaaa aatgcaacaa tagctgattc atctgtagta ggagcatcaa atgacgaagc
     8161 gccaccattt ttacaaaact cgccagtgcc tgttgaccgt agtgtaaata tggaaaaaac
     8221 aacaggccag aaaatacgca ttttgcctta cagagcacgc tttacttcag actatgtggt
     8281 gacgcaatta gacaatagca tcatgacgca aaactatcaa agcttcaatc aaaatttagg
     8341 cagttataat tttccgagct taagtggtat gattaatttt ggcgtaagcg atttaatgga
     8401 agaccataaa atttttggag gatttcgtat tccattcgat tttaaaggct ttgagaccta
     8461 tgtgaaatat gtcaacctga aaaaacgtat cgataaaacc attttgtatt atcgtcgctc
     8521 tgataagatt aatttcaatg tgcaagatgt aaatggaaat accttgcctt atatttacac
     8581 tggaaaaact agaacaaact tttttgaatc tgcctttagt tttccttttg atatcacaaa
     8641 aagtttgcgc tggtcggttt cttacagaaa cgaaagttta gatattgcac tgacagatca
     8701 agtaagcttg gcattgaaag atgttaaaga aaattgggtt tctggtcgtt tggaatttgt
     8761 gcatgataat tctaaagaga ttcaatttaa tatttataat ggctttagat acaaagtatt
     8821 tgcagaatat ttttataatg caaacgagaa gaagtcacac attgcgaata ttggatttga
     8881 tgtgcgccat tatcaaaaag tgtatcgcaa tattatttgg gcaaaccgtt ttgctggtgc
     8941 cacatctttt ggtccaaaaa aaatattgta tttcttaggt ggcgttgata catggttaaa
     9001 tccgaaatat gctgatgata ttcccatcgc tacagaccaa aattatggct tgcaagcgcc
     9061 tgtaatgaat atgcgaggtt tgccacaaaa tattagaaat ggcaataatt acttgctttg
     9121 gaattctgaa ttgcgtattc cattgttttc attttttgct aaaaaacctt tgaaatcagc
     9181 ctttttaaga gactttcaac tgttaggttt ttttgatgca ggtattgctt ataattttgc
     9241 aaatccattc gaccaaaaaa attcacaaag cacagaagtg gttacacctc aacctaataa
     9301 taatcctgtc gtagtaacgg taaattatta tagaaatcca tttgtatttg gcactggtgg
     9361 aggctttcga acgaatttac ttggctattt cttgcgctta gatgtaggct ttggctacga
     9421 tggtttacaa ttcaacaaaa aaccaatgtg gcatttttca ttaagtaaag atttttaaga
     9481 attatcaata attaagcaag ttttgacaag atttgcacaa cattttttta tataaatcac
     9541 aaccttcaac tttatttttt atgctatatt ccatgacagg ttacggaaaa tccactttta
     9601 aattaggtgc caaatcatac gtaatagaaa tcaaatcttt gaatagcaaa tcactcgatt
     9661 tgaattttaa agtagctgca gaattgcgtg gtaaagagat agaaattcgc aaacaaattg
     9721 ccgatcagtt aattcgtgga aaagtagata ttttttttca agaagacaaa agcaccaatg
     9781 gaaacactaa cctaaatttt aatttgatag aatcgtatta tcagcaatta caacaattca
     9841 ctaccgaaaa aaatattcca cttggcgata ttcttccagc gatattgcgc tttcaagata
     9901 tcaacaaaac agaattgaca gagttgaatg acgctgatgt tgaggcattg ctaatgggaa
     9961 tggataatgc cttgaaagaa gtaaacaaat ttaggcaaaa agaaggagct caactagaaa
    10021 gagatttgca agaacgaatt aatattattc agcaacataa aatcgcactt ttaccttttg
    10081 aacagcaacg tatggaaaag ttgcgaagta gattactgca agatttgaag acaaatttgc
    10141 aagaaaatat tatcgataaa aataggtttg aagaagaatt gatttactac atggaaaaaa
    10201 tggatatcaa tgaagaaaaa gtgcgcctcg atgcacatat tctttatttt tttgaaattt
    10261 tgaatgaaca aaacaaagaa aaaggtaaaa aacttggatt tgtagcgcaa gaaataggaa
    10321 gggaaattaa taccattggt tcaaaatcaa acgatgcaaa tatgcagaaa attgtggtgc
    10381 agatgaaaga tgaattagaa aaaataaaag aacaagtact gaatatatta tagtatccat
    10441 aactggaagt aaagaaattg aaaaatctcg attttcgcta tatactactc gtttctaatg
    10501 tatatctttg cacatgcaaa aagtcattat actttcaggt ccttcaggtg taggtaaaaa
    10561 tactttaggc gactttttgc tgcaacaatt tcccgaactt tcttattccg tatcagcaac
    10621 atcgcgtagc attcgcaaag gcgaacaaga tggtgtcgat tatcacttta tgaataatga
    10681 agaattcgaa cagaaaataa aagaagatgc attgctcgaa tggcaagaag tatatgatgg
    10741 catgtattat ggcactatga aatctgaatt agatagaata aacagcctta ataaatttcc
    10801 attattagtg gttgatgtat ttggtgcaat caatgtaatg aagaatctaa agtttaaacc
    10861 actttctatt tttattcaag caccaagctt ggaaatattg gaaaatcgat tgcgtacgcg
    10921 cggcacagac tcagaagaaa agatagcaaa acgattggaa aaagcatctg tcgaaatgaa
    10981 tgaatctgcc aatttcgatt taacaatcat caatgatgac ttgccgatgg cacaaaaaca
    11041 attagaagaa gtagtgcact atttcttgaa atttggtttt taatttttac ataattccag
    11101 tttgtttttt tgattattat ctgattttta taaatcataa ggaaacttaa tgtacaaaat
    11161 attcgttata tttatgtaca acaaaaagat ggtaacggct atcacacata aaataaaagt
    11221 cagcgtagaa acattctacc agtcagatta ttcgtatcca atgagccagc attttatgtt
    11281 tgcctatcgt attactattg aaaataataa caataatacg atacagcttt tacgaagaca
    11341 ttggtatatt gttgatgcac ttggccagca acgcgaagta gaaggcgaag gcgtgatagg
    11401 cgaacaacca attataaaaa gtggcgagcg atacgaatac atttcaggtt gcgatttgaa
    11461 aacggaaatc ggtaaaatgt atggtactta tttgatggaa aacttacatg atggtaaatg
    11521 tttccacgtc gttattccag aattttacat ggtagcaccc atgaagtata attaatattc
    11581 tatttatctt ttcgctcaag taaagtcata aaaaaaccat caccatcata cattgttggt
    11641 agaagttgtt gtgactgctt ttcttgccat tgagctatgt ttttagctaa aaaattttgg
    11701 atttgtaatt gattttcttg cggtaaaata ctacaagtag catatactat tttcccattt
    11761 tttttcaatg tagaaatata tgtagcaagt aaattttctt gtatttgtgt gtattcattt
    11821 attttttcaa cgctaatttt atatttcaaa tctgcttgcc gtttaaatgt gccactacca
    11881 ctacagggcg catcaatcaa aaggatatcg atgtttttta aattctttac agttttgaaa
    11941 tctataattt caatattttt acagcctgct tgcgtggcgc gtaattgtaa attttttagc
    12001 cgagctgcat ggtaatctgt cgcatatatt tttccacgat tatttaataa tgcagataaa
    12061 tgcaacgatt ttccgccagc gccagcacat aaatctaaga tgctaatatt gtctattttc
    12121 gatttgtctt caatagcatg gaataaaaaa ttgccaattt cctgcgagcc tatatcttga
    12181 aattcaaata agccttgttt atagtaaagg ctgtttttta gattgttttt tgataatatt
    12241 tttaagtatg aattttttgc ttgtgcttct atttcaatgc cttctttaag caatatttca
    12301 atcaaatttg cagcagatgt ttttaatgaa tttacgcgaa tataaacgct aggtttaaca
    12361 tttaatgctt ttgcaatttg atgccatgat gcaccaatac tgtctaggca aaaatcataa
    12421 aatgtttttg gataagattg ttcaatatat ttttcatcga ttgctttttg aatgatttcg
    12481 tttaatcgca atacttcact attcgtaata tcaaatagtt cgatgttttt tattgtgtaa
    12541 ttccgattga caaaactcac aaacaccaaa tgtttgtaat aagtaaactg ctcacctaat
    12601 tgtgctaatt gatattcgta ttttaatttc caacgcacaa tatcatatac tgtttcagca
    12661 atagtgctgc ggtctcttgc tccaaaattt ttattttctt gtaatgtatt agctactact
    12721 ttatcggcaa cttgattcgc ttcaaaaatt gctttcaaac aaagcgtaat cgtatttaat
    12781 ataggtggat gtacttttat ttcttgcatg caagcttatg atttacgagt taagcgttta
    12841 attatttcgt gatgtcgcgg aaaatttgct acaagttcat tgcactttgc caattcaaat
    12901 ttttcaaatg aaaaagctgg aataacagta cagccacaaa gcgcaaatcc atattcgcca
    12961 atacattcac tcgcaaacca attgcctttt ctaacaatat gttggaaatg tgcttgctgc
    13021 aaatgattgg ataatttatg ttgatgataa tcgccatttt tttcaatttc gtgaatgatt
    13081 attgcgtcgc cataatacca attccattgc tcatcagatt gaatacgatg aaatgcagaa
    13141 aaattatttt tcaccaataa aaatataatc gaagaaaatg aaccatgttc atcaaagtgt
    13201 gtgcgcttgt aataaccacc ttctggatgt ggctctaagc caaaacgctg tattaattcc
    13261 ttcgcactaa tcatttagat taatttaaac tacaaaatga tattcaactc caaaattagt
    13321 attactttgg ataaactttc aataaatatg caaaaaccga tattggtttt caaaatagga
    13381 acctcatcaa tcactgatgc gtcaggtaat ttagatgaag aagttgtgca aaatattgct
    13441 aagcaattgg ccactttaca caaagaattt caaatcgtaa tcgtgtcttc aggtgctgtt
    13501 ggtagtggaa aaaaatttat aaaaaattat tctggaaaaa tcgaagagcg caaggctgcg
    13561 gcagctatcg gcaatccatt gcttatccaa aaatattctg cggcatttag taaatataaa
    13621 attccaattg cacagagttt atgtgaacgt ggacattttt ctgatagagc taagttttta
    13681 caattacgtg caacgtatga agagctttgg aaaaatggca tcattcctat tgcgaatgaa
    13741 aatgatgtgg tcagctcgcg tgagttgaaa ttttccgata atgatgagtt agccactttg
    13801 cttgcagtag gttttggtgc gcagctattg ttaattggta cttctgttaa aggtgtttta
    13861 gatggcgagg gaaatttaat aaagcgcatc gaaaaatttg atgataaagt gatgtcattg
    13921 gctacgaaag aaacatcggc ggctggctta ggtggcatga ttagcaagct tacgtttgca
    13981 cacatggcaa gtagtttagg tattaaagta catatctttt ctgctaaaga aaaaaatagt
    14041 ttattgaatg ctgtgaaaga aaaaacaggc acgatttgca ttgctaaaaa tgtaagtaag
    14101 tcagcacgtc acaagtggct ggcgagcgga agccttgtga ttggaagcat tactgtcgat
    14161 gaaggcgcac aaaaggcatt actaaaacgc aaaagccttt tggcagttgg cgttacagca
    14221 ataaatacag attttgaaat tggcgaagcc atcgaaatta tggatgttaa tgctaatgta
    14281 tttgcggtag cacgtacaaa aatttcttcg aaaatattgc agaaaaatat taaacaacaa
    14341 aacttgattt tagcacacgc agatgatatt gtgttgattt aactttataa ttttacttca
    14401 gtaaaaggca gcatcatact tgttggaatt tcttgctcgt ttaatctttt cagtacttct
    14461 ggcaaatgtg catcacgcat atattgcaaa ggcttgtcaa atgaaacaaa aaaattattc
    14521 caatgatgca gaattatttc ttttggttgt gtgatgttat atacttctaa taaattatcg
    14581 atattcaatt tttctttcca aaaaatttgc aattgtatat ctgatttgcg ttcattactc
    14641 aatgttttta atgtttgcat ggcatcaatt gtcaaattac cattgactaa tacaattcta
    14701 tattttactc gttgattttc taaaatatca ataagatatg aataggcttc gccttttttc
    14761 cattgccatg gttttgttgg tatttttgct aatggtgtgt catagtgtcc tttaaaaaat
    14821 tctatttttc cgatgtgcgg actgtgcaca ctttttaagg caaaaatgcg aaatgcacta
    14881 tcattttcat atatccattg ttgtttatct gtataatgca aggcagtttt tctaaaagta
    14941 gcagccttgt aaattacatt taattgatta tgtacacttg cgtcagcaat aatttgcgct
    15001 tgcgaaggca gatagttaca taaatctgta atgtcataac aatgatcata atgtccatgt
    15061 gaaatggcta ccatgtcaat gtttttaagc ttttcaggac taattctttc cgaccaagaa
    15121 tcaaattttt tcgaaaaatt aaaaatatta ggattgctaa taaatgggtc gcataaaata
    15181 cttttttctt tattttcaat aataaaacca gcacagccta agtatgtgag cgataagctg
    15241 tcatgcacta gcttgtcttt ggtgaggtag ggcgtgattg tggcaatatc taattttttt
    15301 cctaagaata taaatttcag aaaaatagcc gaagataaaa tcaacaatag aaatacaatg
    15361 atttttgtcg ctcgctttaa cattagatta ttttatgcta aaaatactat aaaaacacaa
    15421 atttgctata aattggtaga aacgaattta tggaatctat tcagtattta ttagaagcaa
    15481 caaataaagc agccgttgaa gtaggaaaat tatcaatagt ttccaaacat gctatactaa
    15541 aagatttagc agcggcattg ttgtcacaaa ctgaaataat tatagctgaa aatgccaaag
    15601 acttacagcg catggaggac aacaatccta aaaaagatag attgttgttg aatgcacaac
    15661 gcatcaaaga tttgtcagat agtttgtatg atattctgaa gttagaagat ccaagtggaa
    15721 aagtattgtc agacaatacg atgtctaacg gcttaaacat tcaaaagatt gctgtgccaa
    15781 ttggtgttgt tggcgtgata tatgaagctc gaccaaatgt aaccatagat gttgccgcat
    15841 tgtgtttata tgctggcaat ggcgtggtat tgcgcggtgg tagtgatgct gaatttacaa
    15901 atgaaatatt ggtcaaaata attcactcag tattagaaaa aaatggcgtc agtaaagatg
    15961 ctgtcagctt gttgccagtt gatagaaagt ttgttaaaga gctattggcg gcagtaaaat
    16021 gggtagatat tattattcca agaggttcac aacagctgat tgattttgta agagaaaatt
    16081 ctaaagtgcc aacgattgaa acaggtgcag gcgtgtgcca tacttatatt gagcgcacag
    16141 ctgatttaga tatggcagca caaattgttg taaatgcaaa aacacagcga ccttcagttt
    16201 gcaattcatt agatactgtt ctagtagata aggatatcgc caaggaattt ttgccaaaat
    16261 tgatagatgg ttttaatgct ttcgatgttg aaatatttgc tgacgaaaca gcatacaaca
    16321 tttttaaaga tagtaactat actcacttgc aacctgccaa tgctgacagt tttggaatgg
    16381 aatatctaag tcagaaatgc tcaattaagg cagtagagag ctttgatgat gcattagcac
    16441 atattcgaaa atactcgtca agacatagtg aagcaattgt aagtaaagac gaaacgcttt
    16501 gcgaaagatt cttaaatgaa gtagatgcag cagctgtgta caccaatgct agtactcgtt
    16561 tcacagacgg aggctgtttt ggtttaggtg ctgaaattgg catttctaca caaaaattgc
    16621 atgcacgtgg accatttgct ttggaaaaat taacaacaga aaaatggaaa gttagaggta
    16681 gcggacaaac gcgttaatta ttcaaaaaca cacaaaacat cataatgtag attggtgttt
    16741 tctaaattat ttattttctc ttgtgctata ccattaatac gatataatgt atatccaagt
    16801 ttgttttgta aataggagag tacttctttt tcagatgtgt tttttgcaga taaataacta
    16861 ttatctattt ctataaataa aataggacga tgttgtaata tcgtttgtgt agcacctttt
    16921 agtacaaaat attcaaatcc ttcgacatct atctttataa aatcaatatg cggaatattt
    16981 tgttgcctta taaaattatc taatgtagtg cctacaacac gttttgctgt acctgcttgt
    17041 tgtttgttta atgaaattcc agcactgttt ttatttgaag gcatttcaaa aaacaaatct
    17101 tcttcattgt cagcaatcgc aatattgaat gcggctatat tattataatc atttaaactg
    17161 atgtttttta taagttgttc aaataaaaaa ggaacaggct cgaaactata tacttttcca
    17221 ttttttgcta tttgtgcaaa attcaatgtc gtttcgccaa tatttgcgcc aatgtctata
    17281 caaacaaaat tagcttctat caaggaatat aatttccgtc tatcaaaatc tacatgctgt
    17341 gtaaggtgaa aatagatagc atgttgcatg taatcgctaa tatctaactc gtagttaata
    17401 ttattacgtg tgcaatgcct aatactgttt tttggataaa gatgattgcc agccagcaat
    17461 ttttgtgcaa ttacatttcc ttgccgcagc tgttctaaaa ttagtttttc tataaatgga
    17521 atacgacata caattattct aacaaaatct aaaatctttg attttatgct cataaataaa
    17581 gaagtatgat gtattcaatt acattctcaa atcttcgaca tgcacaccag gatgttttgc
    17641 cgcatcgaat ttaaagaaac tatcttccaa atttgctttt gaattaaatg atgttacact
    17701 gtaagtatat aaagtgccat ttttatcaaa tactttcata cgcttgatgt atttagcatt
    17761 tttgtctatc caaatacgca ctttgaaaaa tggtttgctt ttatcttttg gcattaaatc
    17821 aatcaccgta cagctcgttt tgtcaacagc ttcttcgcca ttcatcatgt aattaaaatc
    17881 attctggtaa atggtaaaca tattagaagg cgtaatgtct tctttgttcg gctcataatc
    17941 atttatctgt acttcattga tgtcttttaa atatgtccat acactttgct tgtcgcaata
    18001 aatgatttgt ttgccaagac ttaatttata tttttcacct tttaaaaata cattgccatt
    18061 ttgtactgtc gatttgtttt gtcctggatt gtttgttgtt aaggaaaatc cagcttcgat
    18121 ggcattgtag cttttatatg ttttgctaac agcatctaag attgtttttg cttttgggtc
    18181 gttcaattgg ttgccagatt gtaaaccgaa tagcgataaa aaaaatgtaa aaaatgaaaa
    18241 aagttgaatc atatatattt aattagcgta gcacttattt ttattatcta aaatggataa
    18301 attttttaca aaaataaaag ataagttcta ttgttcacct agttcctgca aaattcgttc
    18361 caattcttcg tcggttttta catacacttc tctagccttg ctacctaagt taggaccaac
    18421 gatgctggcg gcttctaatt gatccattaa tcgaccagaa cgagcataac caagcgccaa
    18481 ttttctctgt attaatgagg tcgagccaat ttgatttcgt acaattaatc gtgctgcttc
    18541 tgtaaaaagt tcatctcttt ggctaaaatc gaatgaacgt tctccatttt tatcagcttc
    18601 tttcgggtcg atgtattctg ggagctcgta aggcgtcgga aatccttttt gtgtgcctat
    18661 gaacttggcg atgtgttcca cttcaggcgt gtccacaaac ggacactgca agcgtgtcag
    18721 ttcgccgctt tgtgacaaca acatgtctcc acgaccaatc agctggtcgg ctccgctagc
    18781 atctaatatt gttcttgagt ccacttttgc cgttacttta aatgctattc gtccagggaa
    18841 attcgctttt atagtacctg taataatatt cacagaagga cgttgtgtag caatcactaa
    18901 gtgaatgcca atagcgcgtg cgagctgcgc caaacgagca atcggtgctt ctacttcttt
    18961 gccagcagtc atcatcaagt cggcaaactc atcaaccact aatacaatat aaggcaagaa
    19021 tcgatggcca ttattaggat tgagctgacg ttttaaaaat ttctgattgt attctttaat
    19081 atgtcgtact tgtgcagctt ttagcaattc atagcgtgta tccatttcga tgcacaaagc
    19141 atttaaagta taaattactt ttttattatc tgtgataata gcttcttcct cgtcaggcaa
    19201 ttttgctaaa taatgatttt cgataagctt gtataggttc aattctactt tctttggatc
    19261 gactaataca aactttaatt ctgctggatg ttttttgtaa agcaatgaca ctaaaatggc
    19321 attcagacca acagatttac cttgaccagt ggcgccagcc atcagtaagt gtggcatctt
    19381 ggctaagtca acaacaaaat cttcattgga aatagttttt cctaatgcaa taggcaaatc
    19441 catgtcagat ttttggaatt tttctccagc aattaatgaa cgcatcgata cgatactctt
    19501 tttctcattt ggcacttcta taccaatagt acctcttcct ggaattggtg caatgatgcg
    19561 aatacctaaa gcagaaagac ttaatgcaat atcgtcttct aagtttttta ttttagaaat
    19621 gcgaacgcct ttgtcaggga caatctcata taaagtaacg gttggcccaa cggttgcttt
    19681 aattttttca atgccgatat tataatgtga taaagtctga acaatttggt ttttattttg
    19741 ctctaattct tctttgttaa tttcaatttg accacttcca tgttcttcta ataaatcaac
    19801 atttggaaat ttgtacttag atagttctaa tttaggatca aaattagtgt ctaaaccaaa
    19861 atgttcttta tcggcctcaa aatcggtttc attttctttt tgaatatgtg tccatgcagc
    19921 atttgcattt tcgtctattg tgccaaatgc ttgtatctct tcgatttcga aatcaggctc
    19981 taatgaaaat ttttcttttt tcgatgttgg ttcaatagtt gtattttcga attcaatttc
    20041 ttctgtttct ttttcaatca attccatttt tatttctttg gaattgcttt gtattacttc
    20101 ttcctgttcg tttaatattt caaaagtttc atctttatgg tgaggaattg cttgttcttt
    20161 ttcttcagga atgatgcttt ccatatcata accatcttct tttttcaatg cttcttcttt
    20221 gtaaaaatca tctatttctg ctgtgctgct gtttttattt tctgcttcat attgtgcttt
    20281 ccatgtttgt ttccatcttg taaatttttc atagatatct tttaggttaa aacctaagaa
    20341 tgagaatcca ataattccta ataatattcc aagtcctaaa ttgcctatta aagttgataa
    20401 ccattcatag ttattgcgac ctaatccgcc accaaacgga aagtcgtaat ttgaaaatat
    20461 aaatgatgct aaagtggcaa taaaaactat ccaaaacaga gaactgaaaa atattttagt
    20521 aagcttaaaa attcgttctt taaatgtaag gttgatgccg atgcagaata acaaatatgg
    20581 tataataaaa gaagcaacac caaaaaaaac ataaaagaat acatgagcta gatatgcgcc
    20641 aattctgccc attctatttt ccactaaagc ttctttgcta aatatgattt tccaagaaaa
    20701 atttaacact ttatcttgat catctttcca tgtaaataaa taagagccaa aggcaacagc
    20761 aagcgtgatt gccagaaata aaaaaaacaa tccaaagcct gttttaagtg tatctgtctc
    20821 tatgagtggt gcaggttcac tttcttctac agcctcattt ataggcgctt ttttagtgcg
    20881 ttttactttt tcttttacta cttcctctgc cataactctc aaagataaca gatgtcttag
    20941 tttttagcaa aaatgtataa tgcacagctg gtgataactt cgcttataaa attcctactt
    21001 gtcactatga gtttccttat tttcgaaagc tttcatttct tctttttttt cttgaaaaat
    21061 attaaagaaa agtccaccca ttaaggctcc atataatatg ctattccacg gattggaagt
    21121 aatagcacat gtgccactca agcagccaac ataataataa taggcatagc ctgccattgc
    21181 tcctaataca acacctatga gcgtgagctt atatttttta attaatgtaa tcatgttatt
    21241 tgtttatagc attgttgtcg gacacacata atctgtcaat tcgaatttct ctgatttttt
    21301 aatagcggca aatccaccgg caacgtctat taaattatga taaccacgtg ctcttaatat
    21361 cgaagtgaat atcatggagc gataaccacc agcgcaatgt acatagtaag ttttgttttt
    21421 ctctatttgc tgcatcgtat cattgataaa atctaatggc agattttcag cttcttgtat
    21481 gtgctctgaa aaatattcac tcttcttacg tacatctaaa atggataatg ctggattttc
    21541 agattgtaac tgcgctaatt catcagctgt aatagaagta atgctgtcag tttctttagc
    21601 aactcgctgc catgcagcaa ttccaccatc aagatagccg atggtattat cgtaacctac
    21661 gcgcgacaaa cgagttatga cttcctcttc tcgtccaggt tctgtgatta ataaaatagc
    21721 ttgttttatg tcgctaacta aggtgccgac ccaaacggcg aagctgccat caataccaat
    21781 attaatcgag tttggaataa atgccttatt aaattcacta gggcttctgg tgtctaagag
    21841 taaagcattt gtctcgtttg ctattgcttc aaattcatct ggcgttaatg cacgagttcc
    21901 tctgtcaatt actttttcta tgctatcgta gccagtaata tttaataaaa catttttagg
    21961 aaaataactc ggtggagcta ccaatccatc taacacttgt ttgataaatt cttcttttgt
    22021 caacgatgga tttaaagcat agtttgtttt cttttgattg cctaaagtat ccgatgtttc
    22081 tttgctcata tttttgccac aagcactgcc agcgccatgt gcaggatata cgattaagtc
    22141 atctggtaat ggcattattt tatttcttaa tgaatcaaat aaatgtgcgg ctaatttctc
    22201 ttgcgttaaa tcagaaacaa cgtgttgtgc taaatctggc cgacctacat caccaataaa
    22261 taaggtgtcg ccagtaaata atgcagtttt tttgccatta ctatctgtta ataataaaca
    22321 gatgctttcc atcgtatgtc ctggagtatg aagtacggta atcgtcgcat tgcctaacgt
    22381 gaatacttcg ccatcatgtg ttttatgcac tgcatatcca gtttccatag ccgttggccc
    22441 ataaataatt gtcgcgccag tttcttttgc taagtccaca tgtccagata caaaatcagc
    22501 atggaaatgt gtttcgaaga tgtatttaat tttagcattc ctttttgttg ccataccaat
    22561 gtatggttgt acttctcgaa gtggatcaat tatcgccact tcgccattgc tttcaataaa
    22621 ataggcacct tgtgctaagc aacctgtgta aatttgttct attttcatct tctttctttt
    22681 tcttttgcaa tattaagtat ataactagat agcgaccgtt acttttgtta caaatcgcat
    22741 tttatttata attcatacat taaatttctc aatgtcgttg aattcaattt atttgagtgt
    22801 gaaaacaagc tttttataat tatatttagc gcgaatttta atgaaaatgc aaaaaaaata
    22861 tatactagca cttgaccaag gtacaacaag ttcaagagcc attttgttcg atgcaaatgc
    22921 agcaatagtt acagttgcgc aaaaagaatt tactcaaatt ttccctcaac caggctgggt
    22981 agaacatgat ccaatcgaaa tttggcagtc gcaaatagaa gtagccagag aagccgtagc
    23041 aaaagcaaat attcaaccaa ctgaaattgc tgctatcggt attaccaatc aacgcgaaac
    23101 aactattgtt tgggataaag aaacaggaaa accgatatac aatgctatcg tttggcaaga
    23161 tcgtcgtaca gcaggatttt gcgaccaact taaagcaaga aatttagaat cttatgtgcg
    23221 tgaaaatacc ggcttggtaa tcgacgctta tttttcgggc acaaaaataa attggatttt
    23281 agataatgtc gaaggagcac gacaaaaagc acacgaaggc agattgtgtt tcggcactgt
    23341 agatacctgg ttaatttgga acttaacaaa aggaaaagta catgcaacag attacagtaa
    23401 tgcttctcgc acacttatat ataatataaa ggatttaaaa tgggatgagc aattattaca
    23461 cgacttagat gttccgaagt ctattttgcc agaggtgttg ccatcatctg gtatttttgg
    23521 taaaacgcat gctgatgttt tcttcgatat tgaaattcct atcgcaggcg ttgctggcga
    23581 tcaacatagc gcattgtttg gtcaaacatg ttttgaagca ggaatggcga aaaatactta
    23641 cggtacgggt tgctttatgc taatgaatac aggcacagag agagtattgt ctaagcacgg
    23701 attgctaaca acaattgcat ggtcaatagg cggaaaagta gaatatgcat tagaaggcag
    23761 tgtgtttata gcaggtgctg ccattcagtg gcttcgcgat gggcttaaat tgatagacac
    23821 ggcaccagat tcagaatatt atgcaatgaa ggtcgaagac acaggtggcg tttatgtggt
    23881 gccagcattt gcaggcttgg gtgcgcctta ttgggatatg tatgcaagag gtggcatatt
    23941 tggattgaca cgtggcactc aaaaagcgca tctaatacga gcaacattag agtcattggc
    24001 atttcaaacc tatgatatac tagaagtaat ggaaaaagat gccgacattc agttaaaatc
    24061 cttaaaagta gatggtggcg cttgttctaa taacctgtta atgcaatttc aagctgatat
    24121 tttagaagta aatgtagaac gaccaaaagt aatcgaaacc acagcattag gcgcagccta
    24181 cttggcaggt attgctgttg gtttttataa aaaagaaaat ctagctaata acgaacatat
    24241 acatgctacc ttctcgccaa agatgaaatc agaaaaacgt gaaaaactaa taaaaggctg
    24301 gaaaaaagcc gttgagaggt caaaaaattg ggaagattag cactttttca acacttaagt
    24361 atcaatttgt taaaattgga ttaatgatag cttggtgaag attcactatt tttgcaccta
    24421 attaaaccaa tttaaaatga aaaaattatt cgtattattc gctgctgcta cacttttcgt
    24481 aggtgttcaa gcttgcaaac aaaaagaaga agctgctact gaagaagcta ctgaagtaac
    24541 tacagaagaa gttgtaactg actctgcagc tgttgctgct ccagttgatt cagctgctgc
    24601 tgcacctgct gatacagctg ctacaaaata attcagtttc agtacagaat tataaaaagt
    24661 cccactttgt gggacttttt atgttaataa ctcttgacaa attttaacat ttgaatgctt
    24721 ggtatttttc caaattccct tatttttgaa ttaattaaac caatttaaaa tgaaaaaatt
    24781 atttgtatta ttcgctgctg ctacactttt cgtaggcatg caagcttgca aacaaactgc
    24841 tgaaggcgct gctgaagatg cagctgctgc tactgatgca gtaactgaaa cagctactga
    24901 tgctgctgca actgtaactg aaacagctgg cgctgttgct gatagcgcaa ctgctgctgc
    24961 tggtgctgct gtagatgctg ctgctggagc tgctaaagat gctgctggtg cagctgttga
    25021 taaagctgct gaagcagtta agaaataatc aacttagcaa aatgtaagaa agtcctgaat
    25081 ttattcagga ctttcgtttt tttaggctct tttaaatctt acttattgat aataatcgta
    25141 ggtttcgtat cgctatcctt cttttcatta tccttcttga aaatagaagg catttcaaat
    25201 ccgaaatatt taagcgcagc tattaaaatg aatacagcac cagcctcatc taaatttcca
    25261 attaatggaa tattgtcagg aatcaattca ataaatccag ccgttgggtt tatcaaataa
    25321 agcaccgcaa aaaatgcagc tccaattacg gtaatatctt gttttttcat gtttttcatt
    25381 tcgttttgat gaataataaa agcataaacg actattttct tttaaaagtt cgcccaaagg
    25441 aaaaattaac aatagcatta ctctatttca tctgcaaaaa tttattaaaa tattgtacat
    25501 ttgcaccgct taattatggc gaggtagctc agatggttag agcgcaggat tcataaccct
    25561 gaggtcacgg gttcaattcc cgtcctcgct acaaatccaa ctagtatgta ttagttggat
    25621 ttttttattg gcactagctg agatagctca tttttttgta attttgtttt aaatatttcc
    25681 cgatgagtgc ttatgctttt catccagatt cagctacgca agaagatgtt gatgtactgg
    25741 aaaaagaatt gttagaatat agccatcaac ttatagtgta taatgatgat gtgaatactt
    25801 ttgattgggt aatcgaatcc ttgatggcaa tttgtaagca tacgcacgag caagcaacgc
    25861 aatgtgcttt gtttattcat tttagaggca aatatgcagt acaacacggc gatgaattaa
    25921 cattaatacc gatgaaagat gccttgctcg atagaggcat ctctgccgcg gttgaatctt
    25981 gcaattaacg gttttgccta tctatttact tacagagaag ttggtttttc cacctgttga
    26041 tggtgcagaa aatggcattg tcgcaatagg cggcgatttg tccacagagc gattattact
    26101 agcttatcga aatgggattt ttccttggta tagcgatgaa gaaccgattg tatggcacgc
    26161 gccagaccca agatttgtat tgatgataga ggacttacat gtgtcaaaaa gtatgcgccg
    26221 tgtgttaaat tcaaagaaat ttaaggtaag ttttgatacc aatttccaat ttgttataca
    26281 acaatgtgct actgttgaac gtgttggaca agatggcact tggataacta atgccatgca
    26341 aaatgcattt ataaacttac ataaagaagg ctttgcacat tctgtcgaag tgtggaacca
    26401 agatagaata gtaggcggtc tgtacggtgt atgcttgggg aaagttttct ttgcagagtc
    26461 catgtttcat acagaaagca atgcctcaaa agttgcctta atatgcttgg taaattttct
    26521 gaaacaaata gaagttcaat ttattgacgc acaagtttat acaagtcatg tcgaaagcct
    26581 aggcgctaaa aatataagta gaaacgcatt tatggaatta ctgaaatcag cattaaattt
    26641 tgacactttt cgtggaaaat ggatctagtt tgtgattttt aaagttagat atagcatttt
    26701 accatctgtc atctcaattt tataaattta tattcaaaat ttttaaaaaa accaatgctt
    26761 gcattggaaa taacaacttt atagtaaact ttaggtaaca tgttattttc catgttcaaa
    26821 gtacaagttt gtatctttcg agtctaaaat aattccataa ataatgtcaa cttatcaaac
    26881 tccaacagga atcgaaatta atggcaaata cacatcagaa tttgctgaga tattaacgcc
    26941 agatgcattg gcattttttg ctaaacttca aaggaaacac aacgctcgtc gtaaagaatt
    27001 attacaaaaa cgtgtagatc gccaagtgcg tttagatgca ggcgagttgc ctgatttttt
    27061 agcggaaaca aaagctatcc gcgaaggagc gtggacagtt gcgccatgcc cagcagatat
    27121 tcaagataga agagttgaaa tcactggtcc tgttgaccgc aagatgatta tcaatgcctt
    27181 gaactcaggc gctaaagttt ttatggctga ctttgaagat tcaaatgcac caacttggga
    27241 aaataacatt tcaggtcaaa tcaacttgaa agatgccaac aataaaacta tttctttaga
    27301 atcaaacgga aaatcataca aattaaatga tacgattgcc gctttatttg ttcgtccgcg
    27361 tggctggcat ttattagaaa aacatgtatt agttgatggt gaagtagcta gtggttctat
    27421 ttgggatttt gctttatatt tttatcataa tgcaaagcaa cgcgttgaaa aaggaagtgg
    27481 cgtttatttc tatttgccaa aaatggaatc gcatttagaa gcacgtttgt ggaatgatat
    27541 ttttgtagat gcacaaaatg aattaggtat tcctcaaggt tctattaaag caacagtttt
    27601 gatagagact atcgtggcaa catttgaatt aaacgaaata ttattcgaat taaaagagca
    27661 cagcgctggt ttaaattgcg gtcgttggga ttatatattc tcattcatta aacgcactgt
    27721 aaagaacaaa caattcttgg tgccagatag aatgcaagta accatgacta cgcatttctt
    27781 aaaatcatat tctgatttag taatccaaac atgtcataaa cgtgaagcat ttgctatggg
    27841 cggaatggct gcacaaattc caatcaaaaa tgatgaagaa gcaaataata cagcattagc
    27901 aaaagtacgt gccgacaaag agcgcgaagc taaggctggt catgatggta cttgggtagc
    27961 acatcctggt ttagtgcaaa ttgcaatgga tgagtttaat aaatatatgc ctacacctaa
    28021 ccaaattcat gtaaaacgcg aagatgtaaa tgtgagtgca gcagatttat tacaagtacc
    28081 tgtaggcaca attactttag gtggtttgaa aaataatatc gatgtaggta ttcaatattt
    28141 agaagcttgg ttgcgtggta atggttgtgt tcctatccat aatttaatgg aagatgcagc
    28201 aacagcagag atttctcgta cacaagtatg gcaatgggtt caccatgaag atgcaaaatt
    28261 agatgatggt actaaagtaa ctgcagaatt agttcaacaa ttaattgatg aacaaaaagc
    28321 agcttatcaa actacattag gcgataaata cgttgcttca aaatttgatt tagcaacaga
    28381 attatttact aaaatgtcct tacaagatac tttagaagag ttcttaacat tagaagcata
    28441 taactatata gactaatcaa tcaaaataaa ttcttaattc ttaatttcaa taatttaata
    28501 aaaatggcaa ctaaacaaga aagagcggaa acgctaaaaa aggattgggc aacaaatccg
    28561 cgttggaaag acgttaaaag agattacaca gctgaagagg taatcaacgt gagtggttct
    28621 gtaccagtag aatacacttt ggcaaaaaat ggtgctgaaa aattatggaa taaattacaa
    28681 tcggaagact gggtagctgg tttaggtgcg tacaacggta atcaagctat tcaagaagtt
    28741 actgcaggta tgaaagcaat ttacctttct ggctggcaag tagctgctga tgcaaactta
    28801 tcagcagaaa tgtatccaga tcaaagttta tatcctgcta actctgtacc tatggtagtt
    28861 aaacgtatca acaactcttt gatgcgtcgc gaccaaatcg agtacatgag tggcgagcct
    28921 actcgcgact ggatggttcc tatcgttgca gatgcagaag ctggctttgg tggtaacttg
    28981 aatgcattcg agttgatgaa acaaatgatt gaagctggcg ctgctggcgt tcactgggaa
    29041 gaccaattag catctgctaa aaaatgcggt cacttaggtg gtaaagtatt ggtgcctaca
    29101 caagaggcaa tcaataaatt atctgctgct cgtttggcgg ctgacgtttg tggaacttct
    29161 acattagtta ttgctcgtac agatgctgaa gcggcaaact taatcacctc tgatatcgac
    29221 ccacgtgacc ataaattcat catcgaaggt agccgtgctc cagaaggttt ttattatgta
    29281 aaaaatggct tagagcaagg tatcgataga ggtttatctt atgcgcctta tgctgactta
    29341 atttggatgg aaacaggaaa tcctgattta ggtatggctc gtgagtttgc tcaaggtatt
    29401 catgcacaat tccctggtaa aatgttggca tataactgct ctccatcttt caactgggca
    29461 aaatacatgg atgttaaagc aatgactact ttccgtgaag atttagcggc aatgggctat
    29521 aaattccaat tcattacatt agctggtttc cacgctaata acacagtgat gttcgaatta
    29581 agcaaagcgt atatggaacg cggtatggca ggttactctg agttacaaca aagagaattt
    29641 gctttagaat ctgctggttt ccaagctatc aaacaccaat ctttcgtagg tacaggttac
    29701 ttcgatgcag ttcaacaaat ctgccaacaa ggtcaatctt ctactacagc attagctggt
    29761 tctacagaag cagaacaatt tcattaattg attttgtaaa ctttgtttac tatgttatag
    29821 aaaatgcgca acctaagttg cgcattttta tttaattgca tttgtatgaa tttaattcac
    29881 acttattttc tctaatacat gtcgaattaa attatcagtt gctttgtgcg gatctgcact
    29941 aaaagtgccc aagacgcgat ttgctaatat agcattacaa gaaagtgcat gatggcctaa
    30001 taattgccct aagccatata tggcagatgt ttccatttcc atgttcgtga attttctatt
    30061 gcctggtaaa tttatcgctg ataattcatc tatgtaatgc ttgtctttag gtgcaatacg
    30121 cacggtacgg ccttgtgggc cataaaaccc gcaattcgtt agtgttacac cttttcgcat
    30181 atctttacct atagtttcta aaagtttttc tgaggctttt gaaatagaag gatgaacacc
    30241 taaacttcga acgatgggaa taataggctc aataaattgt ttgtcgcttt cagagatatc
    30301 aaaattataa aatccaagca gaccatctag gccaatgcca tattcactag ctaagaaact
    30361 atcgacttcg atgtcggctt gtagtgagcc acatgtgcca attcttatca gatttaaagc
    30421 tgttaaatta ggctttatag ttcttgtttt taaatcaata ttgaccaaag catctaattc
    30481 atttaataca atatctatat tgtctgtgcc aatgcctgtt gaaatacatg ttagtcgttt
    30541 gctgccaata aaaccagtat gtgtaatgaa ttctcgtttc tgtttcttga cttcaatttt
    30601 gtcaaaataa caagacactt cactgactct atcagggtcg ccaacaagaa aaattgtgtc
    30661 tgcaatgtca tctgcttgca aattcaaatg gtatatactt ccatctggat tgacgattag
    30721 ttcactagct tctatttgca tatgtgtttt attaagtaaa acaaaataca aaaaaaatag
    30781 tgtagttgat ttaaaaatat tatgttctaa tattgaaatt gaaaataaag aagatgcatt
    30841 ttagaacata acaaatcgta tttttataca cgtgattacg ccacatcaaa tattaaaaga
    30901 atactttgga tttgtcgact ttagacaagc acagctcgaa ataattcaat cagtattagc
    30961 taaaaaagat accttggctt tgttgccaac aggtggaggc aaatctattt gctttcaagt
    31021 gcctgcgcta atgcaagatg gcatttgtat tgtggtgaca ccattaattt
//
LOCUS       DAOQQV010000017        30222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_442_length_30222_cov_7.671827, whole genome shotgun sequence.
ACCESSION   DAOQQV010000017 DAOQQV010000000
VERSION     DAOQQV010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 30222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30222
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_442_length_30222_cov_7.671827"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2139)
                     /locus_tag="PLL59_03380"
     CDS             complement(<1..2139)
                     /locus_tag="PLL59_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMY22876.1"
                     /translation="MQELAELNESQYNAVTTIKGPIMIIAGPGSGKTRVLTFRIAYLI
                     QNKIDAFRIMSLTFTNKAAAEMRERISHIVGHEARNLYMGTFHSVFARILRGEAHRLG
                     YPSNFTIYDPDDCKSLIKTIIKEEGLNDKLYKPANVFYRISSAKNALLDAKKYAENID
                     LVADDESSGRPKIKEIYAKYQDRCFKAGAMDFDDLLLKTHELLSKFPDVLDKLQNRFQ
                     YFMIDEFQDTNEVQYQIVKLLAAREENICVVGDDAQSIYAFRGATIANILNFERDYPD
                     LKVFKLEQNYRSTEHIVSAANHIIKQNRAQLDKKIWTDNKGGEKIKVFKTASDTDEAR
                     IVADAIFDQKMRQHFTHSEFAILYRTNAQSRTFEEALRKLNIPYKIYGGLSFYQRKEV
                     KDFISYLRVVVNPNDEEALKRIINYPVRGIGNTSIDRLIVTANEHNLPLWHVVEKPEI
                     IQDMQGRAKQAIRDFSIMIKSFRAMLLSKNAYEIAEYIGKQTGLIQELFKDKTVEGIS
                     RYENIQELLNGIKEYTVEEKQEWEEDEVRPENDLAAYLQQISLLTDMDDKNDDNREKV
                     KLMTIHAAKGLEFTSVFLVGLEENLFPSIMSMNTREELEEERRLFYVAITRAKKILTL
                     SFALTRYKFGQLQYQEQSRFIDEISEENKQFFGQKEKPKPEQKSFFGDDNATKWYLSK
                     QYQQKQEAAIPKPTYEAPKHLTKINSAAKST"
     gene            2197..3465
                     /locus_tag="PLL59_03385"
     CDS             2197..3465
                     /locus_tag="PLL59_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMY22877.1"
                     /translation="MEVENIAIFSKTFIMANGNDSPIKKTWKRLLKNKPAIASLIVII
                     IAIILAIVGPSIAPDKTPDADDQILELANVNPGFSIKLLAVQKNREEKSPSFFHKLLF
                     GKENPNEMIPIVSYAFDDSANIVLQTFEGEGRSPQQKILPITDVCQALSITQSDIKIK
                     GDEASFIAFDDKKQTASLSAMKKEIEQNRILQKKYYLGTDAYGRDIMSRLLFGVRVSL
                     SVGLVAVLISLTIGIFMGSIAGFYRGTVDNLIMWFINVIWSIPTILLAMAIRFAIGDK
                     IPSFLAIFIAVGLSMWVEVARIVRGQVLSVREMEYVQAARGLGFTNTRIIIKHILPNI
                     IGPIMVIAASDFASAILIEAGLSFIGIGVKPPTPSWGTMLNDHRVYLLTEGKAFLALA
                     PGISIMVMVLAFNMLGNGLRDAFDVKGKTI"
     gene            3656..4324
                     /locus_tag="PLL59_03390"
     CDS             3656..4324
                     /locus_tag="PLL59_03390"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HMY22878.1"
                     /translation="MKRTYIHKEGWKIVLTTFTIVLLINLLTNYVFHAGQVVSWIIFG
                     FTFSFFCFVTYFFRNPKRNIITDDDMIICPADGKVVVIEEVFEPEYFEDKRLQISVFM
                     SPANVHVNRAPIAGEVKYSKYHDGKYLVAWHPKSSTENERTSIVIDNGSVDVLVRQIA
                     GKVARRIVYYLEEGDSIEQGADFGFIKFGSRVDMFLPLDTKINVVLGQKVKGGITVIG
                     ELPK"
     gene            complement(4328..5776)
                     /locus_tag="PLL59_03395"
     CDS             complement(4328..5776)
                     /locus_tag="PLL59_03395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMY22879.1"
                     /translation="MRFHYSSIVLLLITFVAIVLLYDFPALWQQLPQGSHLWRQADCW
                     AMTENYQQFNLPFLKPEVYNLQAINGKSVGEFPLTYFIAAQFSNAVFALRFLHTFLFF
                     VGIMATYFIAFFFLQRRFLALCASLLLLTSPLLIFYGNNFLTDVPALAFSYIAWAIFL
                     YAVAKQNNLLLLFSFVFFTIAGLLKASQALNIIIASAFFLIQQKEQIRKKPPIAFLLF
                     FMLSFAVLVLWYAFAKRYNYMHHDKYFFLNLFPVWKLSAYDIGLGAWRMAISWSNNYF
                     WRPTLLLLLIGLGYFIYHRNKLDKSLLQIISLSILFTLVYIILFYQKLIGHEYYYTFF
                     FIAFLFIIIGILKTYNTFHAENIFAHTVLFLLLLLNFILCKQEVASKQLDSRNNPFYG
                     TIAFHECLETLGVDENKIILSLPDDSPNKTLSLLRRKGYTAFNDYRSILQNRQADFLI
                     LGNEDWKDNKTLLKYLTDSIGAYQNITIYKLK"
     gene            complement(5857..8781)
                     /locus_tag="PLL59_03400"
     CDS             complement(5857..8781)
                     /locus_tag="PLL59_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HMY22880.1"
                     /translation="MKKLFISLCVVSMATIGFAQQAYEWSTAKTGDYTYQYVKDDPLK
                     TRFYTLKNGFTVIMSVNKDEPRLQTIIATKAGSNTDPANHTGLAHYLEHMLFKGTDKY
                     GSLDWSKEKPYLDQIDDLYEQYNSTTDEVERAKIYAAIDSTSGIAAKYAIANEYDKLM
                     SIIGAKGTNAFTWYEQTAYVNDIPANQISNWLTIESERFRNPVFRIFHTELEAVYEEK
                     NRSLDNDEWKAQEVLLASLFPTHNYGQQTTIGTIEHLKNPSLKEIRKYYEKYYVPNNM
                     CMVLAGDFDPDKVIKEVDEKFGGFVAKPVEPYKAPIETDITAPIEKTVYGPKDEWVDI
                     AYRLPGANSKEAGLLQFIDRILANGTAGLIDININQQQKTLEAVSYAQSMKDYSMYVL
                     EGKPKQGQTLEEVKSLLLHEMENLKKGNFDESLLQAVKNNFEKEKLQKMESNNNRAYN
                     LLDAFIKNVNWKDELSFYEEIKSYNKQDVQAFANKWFGNNYVVVYKKTGMDSSVIKVE
                     KPPIHEVEVNRNAQSDFVKMIIANKVEPITPVFANFKKDIQQGLSNKNAVYSVVNTEN
                     QLFTFYYYFEMGSNHIKKLPIALEYLNYLNTDRYSSEDLKKEFYKLATDYSTQCEPRT
                     TTIQITGLNKNFAKSVELLEHLFVSCQPDQNKLNAFINDYIQARNNDKLSKGKILAGL
                     TNIANYGIKNPYNNKLSDAELKKLRSEELVMILKNLTKYIHKILYYGPEDNKTLVSSL
                     SMYHKNSIATKALPIPIPFKFRKLTTSEVNFAPYNMVQTEISWNRYSAIYNPILIPTI
                     SLFNEYFGSGMSGIVFQTIRESKALAYSTYAQYGTAFKKGEPNLIRAYVGCQSDKMND
                     AIKGMQELLDSLPYSEKFFEVSKTALKNNFATSRIIKQQLLFSFLDAQRHGINYDIRE
                     KTYKMLDKMTFSDINAFHKSMLSRKPYSLNIIGDEKRIDMAALNKYGKVNKYTLQQIF
                     GY"
     gene            complement(8857..10143)
                     /locus_tag="PLL59_03405"
     CDS             complement(8857..10143)
                     /locus_tag="PLL59_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HMY22881.1"
                     /translation="MKSKIHFNKIESHSLEKGNLILFFNKIPAYTRMTGKIFLFLAFV
                     ICQFTSSYSQDSTISYQIQKNIFSDLEKNKIKYIQKLIDSVGIAAPIPNEVLVKHAAF
                     TTSYNPSHRVPNYVVHVIPKDILYGSATRTNDFRPDPAIKQNMSDSADYWYSGFDRGH
                     LAASADFKWNRYALSESYFYSNIIPQNKELNQGAWNKLEMQVREWAIDNNELIVVTGP
                     ILQDNLPKIPQGSFKVSIPEYIFKIVVDYYAPDFKAIAFIYPNKNVPYELDKHVVSID
                     SIEKLSGIDFFPKMENEIENTLEAQHDITEFEKNYFKNSSSKISSKNYGKGKINTTQV
                     KEYIDKQACVCGKVVSTKYNENGKTNPTYINLDKKYPEHVFTIMIFGQDRSHFSYVPE
                     VYLQGKEICVKGKISEYKGSPQIIANKENQIEILSK"
     gene            complement(10292..12055)
                     /locus_tag="PLL59_03410"
     CDS             complement(10292..12055)
                     /locus_tag="PLL59_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HMY22882.1"
                     /translation="MQVKRLFDVLHFQKANHPQQVAIATVKNGEWWKLSTDELIQQSN
                     NMSLGLLKLGINKGDKVAVVTSANRTEWNICDQGIGQIGAINVPLYPTISTKDYEYIL
                     NHAEAKICIVSDAKLYEKVKDLKASVPSLQGIYTFDQVEGAACYEEIIKAGEGGNVAD
                     IKAISDTVDEKDLATLIYTSGTTGTPKGVMLSHQNIVSNVLTVRAELPIEAGHVALSF
                     LPLCHIFERMVIYTYLHTGANVYYSDIDTLKDKLGTVRPHFFTTVPRLLEKVYEGIVN
                     KGLALTGLKKKLFFWALEMADKYDYDQKPSFTFKIADKLIFSKWREGLGGRIMGIVTG
                     SAACPLKMARVFSAAGIPIREGYGLTETSPVLTFNRFAEGGAMLGTVGMPIKDVEIKI
                     AEDGEILAKGPNIMMGYYKEVEKTNEVLTEDGWFSTGDVGTFVTNNAGNKFLKITDRK
                     KELLKTSGGKYVAPAPIESKFRENFFIEQIMVIGESKKFVSALIVPAFPVLEQFATEN
                     DITFASRDELIANPKVQAKYQSIIDEVNPNFSHIEQIKKFKLLKSEWTPETTELTPTM
                     KLKRKVINEKYNKEIDSIYND"
     gene            complement(12063..13832)
                     /locus_tag="PLL59_03415"
     CDS             complement(12063..13832)
                     /locus_tag="PLL59_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HMY22883.1"
                     /translation="MPYSRIFDIIAHQQINSPLEKCLSRREDKKTWTHYSTQTFIETA
                     NQLSQGLLQLGLQKGDTVAIISTSNRPEWHFTDLACMQIGVVDIPVYPTISSKEYEFI
                     FNHAEVKYVFVSDKLMYRKIAQIIPLVPSLKGVFSFDVVDNVTNWKTLLTNDKILVEK
                     VNTIKQSIQASDLATIIYTSGTTGIPKGVMLSHYNIVSNVKDCLKAIPVGKGEVVLSF
                     LPLSHVFERTINYVYFAGSVSIYFADGLETISEHLQDIKPNYFTTVPRLLERVFEKII
                     KKGQTLNGFKKNLFFWSVAQAQNTELGKTRTLAESTNLYIANKLVFNKWREALGGNIK
                     AIICGSAPLQARLATIFTNAGIPILEGYGLTECSPVVSVVPFRQQEFRAGCVGKLLSS
                     VQIKIAEDGEILIKAPNVMLGYYKNEEETKKSFNNEGWLLTGDIGEFTSDGYLKITDR
                     KKELFKTSGGKYVAPSPIENKLKESFYIEQIALQGDGEKFISALIIPNFENLREWAAK
                     NEIKALSNEELIENQQIKELYKRIITELNVNFGKVEQIKHFELLADEWTVENNMLTAT
                     MKLKRKKITEKYKDLIHSFYHKL"
     gene            14024..17128
                     /locus_tag="PLL59_03420"
     CDS             14024..17128
                     /locus_tag="PLL59_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HMY22884.1"
                     /translation="MEKKSSLIIYKASAGSGKTYALAKEYLKIVIQNPYDYKRILAVT
                     FTKKATAEMKSRIVEYLILLEKKSDTVDTLRNSIIDEIKEANGIDVSAVFDKHTRIAL
                     QLILHDYSHFNVSTIDSFFQSIIRAYAKELDLPIGMEVELDTASVIEEAIQAVLKEYK
                     TDNDTFSSWIEAYIFEKIDDDKGWNIEKNIASLAKELLNEDYRLLTEDRQQSFSIDNY
                     KETRLALKKIVANYRQAMDDLVQNFEQEIERQAIDLRLFKGKSTFVSFVSKIKNDAPE
                     FTDTILNMMASDEVLSKETAKDSVLKITMETAWHNTVKPFLLAIANLIENKSKQYHAA
                     IIVLKNIYSLALLEMINEKIKAYKQENNLILISDTNQIVSLIARHEDVPFIFEKSASF
                     LKYILIDEFQDTSTLQWHGLLPLLLEILQSTEGLVLIVGDPKQSIYRWRGGKMELMID
                     GISSAFTDYLEPKNQKTLSVNYRSAQEIVDFNNAFFSTLKNIIPLDNPYFERVLADVE
                     QKAKKTKIKGYVRCQWLDPIKGEDVCLPAILAIIKEVTAYKKHSDIAILVRTNIQGNV
                     VANYLQEQGVPVMSAESLLLNGQANIALLLSALTYILHSDEDFYVIQFNYLLAQYLQK
                     PSLENYLQKQNKQYFFENEFPLFQRKNTSTLRSISINELVFQVAQALQFDTQLDEYLL
                     RFQDVAHQFAQQRSTSLSDFINYWNENEDKLSILPPEGIDAVNIMTIHKSKGLQFPIV
                     IMPYANQELKPKSESLIWINSDEKPFNKLNAFPVNLTSKTKDSLFEAEYLKEFSATYL
                     DNINILYVAFTRAEEQLYVLADASKSDSKDKSPLPNFMNKLIHKVVQSMHLENAQFEA
                     NCFTYGTVNTNKQAPEEINTTAYLSPQHFTDFKTKVPLAITTSYNEAQEKGTKMHEVL
                     AKIKQKNQWKNAIASTVTDDDGYYAQQTQLVLQFFEKQQWFDEKWQHINERSFTFNGE
                     FMRADKILLSADSCIIIDYKTGAKENTHIHQLQNYKAAYATFVPQKVSAFLLYIDTLE
                     LQEI"
     gene            17137..18081
                     /locus_tag="PLL59_03425"
     CDS             17137..18081
                     /locus_tag="PLL59_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HMY22885.1"
                     /translation="MHTIHQQYENIFEIITLANVHHTAKVVVNIGNSLFSYTKNRLEY
                     LHFPFDLQEYANNDKLAGNPFMHPWANRLESNCIRVENLQYNFPIQQQHLLYRDGNQL
                     PLHGLLLKSNKWKTITTQASASQCKHVAAYIFDGEDELSIFPFLHNIQMTHILRDDTL
                     HIETVIHNLDKKDMPISFGFHPYFKKLDAHAKLHLAASNKIETNSLMIPTGQVTAKEK
                     CWNFINHEIAIDDVMFDDGFQDLLFENGMADFSLDNIHVLFCDQYPFAQVYSPSTIDK
                     PYVCIEPMTAPTNALNSNACQLLKSNETFSASFSIKIA"
     gene            18218..18787
                     /locus_tag="PLL59_03430"
     CDS             18218..18787
                     /locus_tag="PLL59_03430"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMY22886.1"
                     /translation="MNKFLLTTAFFFTAFFAVHAQMPAIGTKATELNYKDPSGKEIAL
                     SSLKGNMVLLDFWASWCGPCRRNNPALVALYSKFQNKKWVKGVKGFTVYSLSLDQNAE
                     NWKAAIQQDGLVWPYHVSDLKGWNAEGAAKYGIRSIPQTVLIDETGYVIAINPSHNLV
                     DQLVSKKLSSGTTPSKTKSKTKSSKSIKK"
     gene            18851..19423
                     /locus_tag="PLL59_03435"
     CDS             18851..19423
                     /locus_tag="PLL59_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMY22887.1"
                     /translation="MIEEKETIVDAQTEQQVVEENKNQSESQQESKNAANEFAAEDEL
                     SAFKKEIEEQKDKYLRLFAEFDNFKKRNAKERLELFKTAGQDIVRELLPVLDDFNRAD
                     KVYQQDNNAENFVVGLKLIQEKFHKTLQNKGLKAIDSIGKDFDVAQHEAIAEVPAPTP
                     ELAGKILDEVETGYTLNDTIIRYAKVVVGK"
     gene            19483..20634
                     /gene="dnaJ"
                     /locus_tag="PLL59_03440"
     CDS             19483..20634
                     /gene="dnaJ"
                     /locus_tag="PLL59_03440"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMY22888.1"
                     /translation="MAKRDYYEVLGVAKGASADDIKKSYRKLALQYHPDRNPGDKVAE
                     EKFKEAAEAYDILSNDEKRARYDRFGHQGAAGGFGGGQGMNMDDIFSQFGDIFGGGGD
                     GNPFESFFGGGGRRKERGRGQRGSNLRVKVKMDLEEIANGAQKKIKVKKHIVCDTCSG
                     IGAKDSSSYQTCNTCNGSGVVRKVTQTFLGQMATTGTCPTCEGEGKIITNKCTKCRGE
                     GRVYGEEIVSIDIPAGVSEGIQLSMNGKGNAGIRGGYPGDLLINIEETPHPELKREDT
                     NIIYHLYLNFAEAALGTQAEVPTIGGSAKIKIPKGTQSGKLFRLQGKGIPSLNGYGKG
                     DQIVEVQVFTPQDLTTEETALLEKLRDSKNFNPQVQKEKNKGFFDKFFH"
     gene            20719..21618
                     /locus_tag="PLL59_03445"
     CDS             20719..21618
                     /locus_tag="PLL59_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMY22889.1"
                     /translation="MLDVQHISKTFQQNVALQDVSLSIDKHKIYGLLGPNGAGKTTLI
                     RTITQIFFPDSGHLFFNGEPLAEKHQNLIGYMPEERGLYKKMKVGEQLMYFAQLRNFT
                     KAQATEKINFWLKKLDIESWRNKTIEELSKGMQQKVQFVATVLHNPTLLILDEPFSGL
                     DPINADLIKNEIFELQQAGTTIIFSTHRMENVEEICKDIFLINKGKILLNGNVQAIKQ
                     EYKKHLISVETANKVVLNDAVRSALHVHEQSDKRIVFKLTENQTPNDMLKLLLQQQIE
                     IVAMAEILPTVHEIFIQTVKENS"
     gene            21679..22986
                     /locus_tag="PLL59_03450"
     CDS             21679..22986
                     /locus_tag="PLL59_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMY22890.1"
                     /translation="MNKVWYIIQREYLTRVRKKSFIILTLLLPVLIGLMSVALIEIAS
                     NAQEELTIAVKDDNAWFEDKFRGMDIGDNLRFKYLTNNPQPIDSLKHLYQNIDCDGIL
                     YIPKLDLNQPRGIAYYSENELGMATEKYITEKLSEEIRKRALSQRNYNIDTIEHITQA
                     IDIEKIIDNKKQSGGSEIAGVFGYACGFLIYIFLIVYGAMVMKSVTEEKTNRIVEVLV
                     TTVKPFQLMIGKIIGIGAVGLTQFLLWGILTFGINLLLPFFLGDKMMAMQSMQQTNAM
                     AVSTEESTQLLMNISNAFQTLEIGRIIFSFLFYFIFGYLFYASQFAAIGAAMTDDSDA
                     QSFTFPITIPIIISMVLMSVTLSQPFSSAAFWGSMLPFSSPIIMITRIPFHVPWWEQI
                     LSMFILFLSAMAMVYVAAKIYRVGILIQGKKVSFKEISKWIFY"
     gene            complement(23085..24110)
                     /gene="galE"
                     /locus_tag="PLL59_03455"
     CDS             complement(23085..24110)
                     /gene="galE"
                     /locus_tag="PLL59_03455"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HMY22891.1"
                     /translation="MKVIVTGGTGYIGSHTIVDLIQHGFDVVCIDNLSRSSTYALTGI
                     EKITGKTIPFYRIDLCDANATHDVLSAHQDAVGIIHFAALKSVPESVAAPLLYYKNNL
                     ESLVNILESIEKLGIPNFVFSSSCSVYGNADVLPVTENTPIKNAESPYAHTKQIGEDI
                     IRNFSKRYPAQASILLRYFNPIGAHPSAHIGEIPIDKPNNLVPYITQTAIGKLEQLCV
                     YGNDYNTRDGSCIRDYIHVCDIAHAHTLALQYLVNKRNDSNCEVFNLGSGIGVSVKEA
                     IDAFEKVSLQKLNYVIGERRAGDVAAVYADNTNATTQLGWQCQYSLDDAMLSAWNWEK
                     KMQVLQL"
     gene            complement(24147..25010)
                     /gene="rfbA"
                     /locus_tag="PLL59_03460"
     CDS             complement(24147..25010)
                     /gene="rfbA"
                     /locus_tag="PLL59_03460"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HMY22892.1"
                     /translation="MKGIILAGGSGTRLYPITFAISKQIMPIYDKPMIYYPLSVLMQA
                     GIHEILIISTPQDMPNFQRLLGDGKKYGCHFQYAIQEVPNGLAQAFVIGKSFIGNDKV
                     ALVLGDNIFYGSGLGDLLEKNNDPDGGVIYAYHVSDPERYGVVAFDKKFNAISIEEKP
                     LHPKSNYAVPGLYFYDNEVVSIAENLQPSARGEYEITDVNKIYLERGKLKVGILDRGT
                     AWLDTGTFDSLMQASQFVQVIEQRQGLKVGCIEEVAYRKGFISKEELQAIAQPLLKSG
                     YGQYLLDILNE"
     gene            25164..25850
                     /locus_tag="PLL59_03465"
     CDS             25164..25850
                     /locus_tag="PLL59_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMY22893.1"
                     /translation="MAEKTFKILVVDDEPDILEFLSYNLEKEGFVVETAENGKQALEK
                     AKKNQPDIVLLDVMMPEMDGIEACRTMREMPQFEHTIIAFLTARTEDYSQIAGFETGA
                     DDYISKPIKPRVLVSRLKALLRRYEAKESKSSFLDVGNIQIDRERYLIIFNGKEMAVP
                     RKEFELLYLLASKPGKVFKRDEILNEIWGRDIIVGDRTIDVHIRKLREKLGEDLIKTV
                     KGIGYKFEEI"
     gene            25847..26893
                     /locus_tag="PLL59_03470"
     CDS             25847..26893
                     /locus_tag="PLL59_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMY22894.1"
                     /translation="MRRSASPNIISLLASMAVAIFSTAIFLFITAIFHLKINYSVYII
                     NIFIIFLVTLLLFRFVLVRFIYSKIKIIYKTIGKPLKFQNEVKEGSKNVLKTVERDVA
                     EWAINKNKQIRELRKMEQYRKEFVGNVSHELKTPIFNAQGYIETVLDSDLDDPAFIKQ
                     YLEKANSNIERLETIVTDLLEISKFESGRIELNKEAFDLVKLIKKVFFQYQHIAQQQK
                     ISLKCHSEENAVFVFGDSKRIMQVLENLISNSIKYGKENGHTDIYFHDLEDQYLVEIV
                     DDGPGISSEHLPRLFERFYRADKSRNRKINGTGLGLSIVKNIIEAHEQTINVRSELEN
                     GTTFSFTLQKYIPE"
     gene            complement(26894..28207)
                     /locus_tag="PLL59_03475"
     CDS             complement(26894..28207)
                     /locus_tag="PLL59_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HMY22895.1"
                     /translation="MRKLFTHIIFVISMFLLNITFIQASVKSTFTYANNTNFAGQEEA
                     IKADLYKPNLFSTQRPVLIVYHGGGYAAGDKNLAALKIFADYFTSKNISVILPDFRQG
                     WYESTSKPLCESVTPEYFEDAAHRAYQDNRALIRFCKANANTLGIDSNKIFLMGISSG
                     GFLVLHHLYMQDVLITQERLSRLGSLDLQGNAYRNSTEIAGIISIVGGFYKNDVNIVH
                     PYPTLLFNNSCDGAVDFFNGWLGNCSNTIRSYGPGIFTPILEQQNVPYQFHVFCGYNH
                     GFNSLSAPLGGDADAINYINNKSFDFINNITNGTIETNTSIASDSINARPISECRNFE
                     TFYWCKTDSIAVGSDFISITPNPISCQIAPKLNIRHEKDELLTIEIIDISGRLISSKQ
                     INYNKSNEIIYLQNRDFVLGVNIVLIKDAAKKVLYKTKVMRYCEY"
     gene            complement(28200..28652)
                     /locus_tag="PLL59_03480"
     CDS             complement(28200..28652)
                     /locus_tag="PLL59_03480"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HMY22896.1"
                     /translation="MKVLMVCLGNICRSPLAEGILRHKCAQQKLDWYIDSAGTGKWHI
                     GFGPDYRSVQIANKHGIDISGQRARSVHSADYEEFDLIFAMDTANYKDLLGWALDKKE
                     ESKIKLILNEVYPGENRSVPDPYFDDNGFQNVYDMLDKACDKIIEKYA"
     gene            28717..29550
                     /locus_tag="PLL59_03485"
     CDS             28717..29550
                     /locus_tag="PLL59_03485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMY22897.1"
                     /translation="MKRFILLLFIMFPMFSSTNAKKHWQKTASGICYKLYTKDKSQVR
                     PKIGDYIWMHLVKYSPTKKEIFNTRIFDVGNGVEMQLKAVQKQVDVTEIFTHMRKGDS
                     ALVKIPCHLLDSNGSKKKHYTFRLQLIDFSTKEAYEKVQWEKEFLQAQADSLVMIEYL
                     SMHNMLDALKDAAGNYFVRQIRGNGAQLQIGDSVQLHYIGKLTTDFEFDNSYGRKQTL
                     AFVVGKQQVIEGLDKGICNFYYGDKGILLIPSRLAYGDKSVGKIPANAVLVFDIDILP
                     K"
     gene            complement(29598..>30222)
                     /locus_tag="PLL59_03490"
     CDS             complement(29598..>30222)
                     /locus_tag="PLL59_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMY22898.1"
                     /translation="KEQIDKAGFPSSVKIEYLGEQKNQGDSFGSLGIAMGLGILLVYL
                     IMVALYESTLYPFVVLFSIPVALIGAVLALALTHESLSIFGIIGFIMLMGLVAKNGIL
                     LVDFTNHLKAQGLPLKEALIEAGKERLRPILMTTVAMIFGMLPIALSNGPGAEFKRSM
                     AWVIIGGLSSSLLLTLVVVPTVYYIFDRIKEKIQGKKSEKTVVETIA"
BASE COUNT         9917 a         5159 c         5357 g         9789 t
ORIGIN      
        1 tgtcgattta gcagcgctat ttattttagt taaatgtttt ggtgcttcgt atgtaggttt
       61 tggtatagca gcttcttgct tttgttgata ttgcttgctt aaataccatt ttgttgcatt
      121 gtcatcacca aaaaaagact tctgctctgg ttttggcttt tccttctgac caaaaaattg
      181 tttgttttct tccgaaatct cgtcaataaa acggctctgt tcttgatatt gaagttggcc
      241 aaatttatat cttgtcaaag caaaagataa ggtcaatatt tttttagctc gtgtgatggc
      301 aacataaaac aaacgacgtt cttcttccag ctcttctcta gtattcatag acataatcga
      361 aggaaataga ttttcttcca agccaactaa aaacacacta gtaaattcca aacctttggc
      421 agcgtgtatt gtcattaatt ttactttttc cctgttatcg tcatttttgt cgtccatgtc
      481 agtcagcaaa gaaatctgct gcaagtaggc agccaagtca ttttctggtc gcacctcatc
      541 ttcttcccat tcttgttttt cttcgacggt atattcttta ataccattta aaagctcttg
      601 gatattctca tatcgcgaaa tgccttctac ggttttgtct ttaaataatt cttgaataag
      661 accagtttgt ttgccaatgt attcagctat ttcgtatgca ttttttgaaa gtaacattgc
      721 tcgaaagctt tttatcatga tgctaaaatc gcgaatagct tgcttggctc tgccttgcat
      781 atcttggatg atttcaggct tttcgacaac atgccacaat ggtaaattat gttcattagc
      841 cgtaacaata agcctgtcaa tgcttgtgtt tccaatgcca cgaactggat agttgataat
      901 gcgttttaat gcttcttcgt catttgggtt gaccacaaca cgtaaataag aaataaaatc
      961 tttcacttct ttgcgctgat aaaatgacaa gcctccatat attttatacg gaatatttag
     1021 tttgcgcaat gcttcctcaa atgttctcga ctgtgcgttg gttcgatata aaatagcaaa
     1081 ttcggaatgc gtgaaatgtt gtcgcatttt ctgatcaaaa atagcatcag ctacaatacg
     1141 tgcttcgtca gtatcgctgg cagttttaaa aacttttatt ttttcgccac ctttattgtc
     1201 cgtccaaatt tttttatcaa gctgtgctct attttgtttg atgatatgat tggcagcgct
     1261 aacaatatgt tctgtagaac gataattttg ttcaagctta aacactttca agtcaggata
     1321 gtcgcgctca aagtttaaaa tatttgcaat ggtagcacca cgaaaagcgt aaatgctttg
     1381 tgcgtcgtct cctacaacac agatattctc ttcgcgtgct gccaatagtt ttacgatttg
     1441 atattgcact tcattggtgt cctgaaactc atctatcata aagtattgaa agcgattttg
     1501 tagcttgtct aatacgtctg gaaatttaga taataactca tgcgttttta acagcaaatc
     1561 atcaaaatcc atagcgcctg ctttaaagca tctatcttga tattttgcat aaatctcttt
     1621 gatttttgga cgtccgctgc tttcgtcatc agccactaaa tctatatttt cagcatattt
     1681 tttggcatct aataaggcgt tttttgccga agaaatacga taaaatacat tagctggctt
     1741 atatagcttg tcatttaatc cttcttcttt aattattgtt tttatcaaac ttttacaatc
     1801 atcaggatca taaatagtga aattacttgg atagcctaaa cgatgcgctt cgccacgtag
     1861 tatgcgtgca aacacggagt gaaatgtgcc catatataaa ttacgagcct catgaccaac
     1921 aatatgtgaa atacgctcgc gcatttcagc cgccgcctta ttggtaaagg ttaatgacat
     1981 gatgcgaaat gcatctatct tattttgaat aaggtaagca atgcgaaaag tgagcacacg
     2041 cgttttccca gaaccaggac ctgcaataat catgattggt cctttaatcg tggttactgc
     2101 gttatattgt gattcgttta attcggctaa ctcttgcatg gtgtgcaaag atagtttttt
     2161 attgataaaa aaaattggca cacagcaagt cgcagtatgg aagtggaaaa tattgctatc
     2221 tttagcaaaa cttttatcat ggcaaatggc aatgattcgc ctattaaaaa aacatggaaa
     2281 cgcttgttaa aaaataagcc ggcaattgcc agccttattg tcattattat tgcaataatt
     2341 ttagccattg ttggcccaag tattgctcct gacaaaacac ctgatgctga tgatcaaatt
     2401 ttagaattgg caaatgtaaa tccaggcttt tcaataaagc ttttagccgt tcaaaaaaat
     2461 agagaagaaa aatcgccatc tttttttcat aaattattat ttggcaagga aaatccgaat
     2521 gaaatgattc cgatagtttc ttatgctttt gatgatagtg ccaatattgt attgcaaaca
     2581 tttgaaggcg aaggcagaag tccacaacaa aagatcttgc caattactga cgtctgtcaa
     2641 gcattgtcta ttacacaaag cgacatcaaa ataaaaggcg acgaggcaag ttttattgcc
     2701 tttgacgata aaaaacaaac ggcatcttta tcagcaatga aaaaagaaat cgaacaaaat
     2761 cgaatcctac aaaaaaaata ttacttaggc acagatgctt atggtcgtga tataatgagc
     2821 cgtttgttat ttggcgttcg tgtatcatta tctgttggct tggtggctgt tttgatttca
     2881 ttgaccatcg gcatatttat gggcagtatc gctggcttct atagaggcac tgtcgataat
     2941 cttatcatgt ggtttattaa tgtcatttgg tcgataccga ctatattatt agcgatggcg
     3001 attcgatttg caattggcga caaaatacct tcgtttctcg ctatttttat tgcagtagga
     3061 ttgtcgatgt gggtcgaagt ggcgcgtatt gtgcgcggcc aagtgctcag tgtacgcgaa
     3121 atggaatacg tacaagcagc gcgtggctta ggttttacca ataccagaat tatcataaag
     3181 catattttgc caaatatcat aggtccaatt atggttattg cggcatcaga ttttgcatcg
     3241 gccatcttaa tcgaggctgg attgagcttt attggcattg gcgtaaagcc accaacgcct
     3301 agttggggaa caatgttgaa cgatcaccgt gtttatttat taacagaagg taaagcattt
     3361 ttggcattgg cacctggtat ctctattatg gtaatggtat tggctttcaa tatgttagga
     3421 aatggacttc gagatgcctt tgatgtaaaa ggtaaaacaa tttaatgcta actaatagat
     3481 aatcatataa ttacacttta atctgattat ttatgcacaa atcaaaaaaa tatattgcca
     3541 aacattaaat ttatattgaa ctacgtatgt taaatgcgtg ttctattgct gtattcagta
     3601 tatatactta cttttggtga ctgtttaagt tcatagaacc caaaaatgaa ttgaaatgaa
     3661 aagaacatat atacataaag aaggttggaa aatagtattg actaccttta cgattgtttt
     3721 gctaatcaac ttgctaacga actatgtatt tcatgctgga caagtagtga gctggattat
     3781 ctttggtttt actttttcct tcttttgctt tgttacctat tttttcagaa atccaaaaag
     3841 gaatattatc acagacgatg acatgattat ttgtccagct gacggtaaag tggtagtgat
     3901 agaagaagta tttgagccag aatattttga agataaaaga ttgcagatat ctgtatttat
     3961 gtctccggca aatgtacacg ttaaccgtgc gccgattgct ggtgaagtga aatattctaa
     4021 atatcacgat ggcaaatatt tagtagcttg gcacccaaaa tcatctacag agaatgagcg
     4081 tacttcaatt gttattgaca atggaagcgt agatgtgtta gtgcgtcaga ttgctggaaa
     4141 agtagcgcga cgtattgttt attatttaga agaaggcgac agtatcgaac agggtgctga
     4201 ttttggtttt attaaatttg gctcacgagt agatatgttt ttgccattag acacaaaaat
     4261 caatgtagtc ttaggtcaga aagtaaaagg tggcattaca gtgattggcg aattgcctaa
     4321 gtaattttca ttttagttta taaatagtaa tattttgata agcgccaatg ctgtcagtca
     4381 aatactttag tagtgttttg ttgtctttcc aatcttcatt tcctaaaatc aaaaaatcag
     4441 cttgtcggtt ttgcaagatg cttctatagt cattaaatgc agtatatcct tttctgcgaa
     4501 gtaaggataa agttttattt ggcgaatcgt caggaagtga tagtataatt ttgttttcgt
     4561 caacacctaa tgtttccaag cattcatgaa aggctattgt tccataaaat ggattgttgc
     4621 ggctgtctaa ttgtttgctt gctacttctt gcttgcaaag tataaaattg agcaatagca
     4681 aaagaaaaag cacggtgtgt gcaaatatat tttctgcatg aaaagtatta taggttttta
     4741 agatgccaat aatgatgaac aagaaggcaa tgaagaaaaa tgtgtaataa tattcatgtc
     4801 caattagttt ttgataaaac aaaataatat aaaccaaagt aaataaaatg gacaaggaaa
     4861 taatctgcaa taaacttttg tcaagcttat tacgatgata aataaaatag cccaatccga
     4921 tgagtaataa cagtaaagtt ggtcgccaaa aataattatt gctccaagaa attgccatgc
     4981 gccacgcacc taatccaatg tcgtaggcgc tcagtttcca tacaggaaat aggttcaaga
     5041 aaaaatactt atcgtgatgc atgtaattgt agcgcttagc aaatgcatac cacaatacca
     5101 acacagcgaa agacaacata aaaaacaaca aaaatgcaat tggtggtttc ttgcgaattt
     5161 gttctttctg ttgtattaga aaaaatgcac tggctataat aatatttaaa gcttgacttg
     5221 cctttaataa accagctatg gtgaaaaata caaaagagaa aagtaataat aaattgtttt
     5281 gttttgctac tgcgtataag aaaattgccc aggcaatata ggaaaatgca agtgctggca
     5341 catcagtcaa aaaattatta ccataaaaaa ttaatagcgg tgaagtgagc aataataagc
     5401 tagcgcaaag tgctaaaaat cgtctttgca aaaagaaaaa ggcaatgaaa taggtagcca
     5461 tgatgcctac aaaaaacaaa aatgtatgta aaaaacgcaa ggcaaaaacg gcgtttgaaa
     5521 attgagccgc tataaaatat gtgagcggaa attcaccgac tgatttgcca tttatcgctt
     5581 gcaaattata cacttcaggt ttcaaaaacg gcaaattaaa ttgctgataa ttttctgtca
     5641 ttgcccagca gtcggcttgc cgccaaagat gcgagccttg tggtaattgt tgccataatg
     5701 caggaaaatc atacagtaga actattgcta caaatgtaat taacaatagc acaatggaac
     5761 tgtagtgaaa tctcatacaa taaaaataca gttatataaa ctaaaaacaa aaagccagac
     5821 aaatatgtct ggcttgtaat ggtgattatt tagcgtttaa tagccaaata tttgttgaag
     5881 tgtatatttg tttactttgc catatttatt taaggcagcc atgtctattc ttttttcgtc
     5941 gccaatgata ttcaaactat atggttttct agataacata gatttatgaa atgcgttgat
     6001 atcgctaaat gtcatcttat ccaacatttt ataagttttt tcgcgtatgt cgtagttgat
     6061 accatgacgc tgagcatcta agaaactaaa caataattgt tgtttgataa tacgtgaagt
     6121 agcaaaatta tttttcaaag cagttttgct aacttcaaag aatttttctg agtaaggcaa
     6181 tgaatctaat aattcttgca tgcctttaat ggcatcattc attttatcag attgacagcc
     6241 tacataagca cgtattagat taggttcacc ttttttaaat gcagttccgt actgagcata
     6301 cgtagaatat gcaagtgctt tcgattcacg aatcgtttgg aaaacaatac cagacatgcc
     6361 actgccaaaa tattcgttaa acaatgaaat agttggtatc aaaataggat tataaatcgc
     6421 agaatatcga ttccagctaa tctcggtttg caccatattg taaggagcaa aattgacttc
     6481 gctcgttgtt agttttctaa atttaaaagg tatgggaatc ggcaatgctt ttgtggcaat
     6541 agaattttta tgatacattg acagactaga taccaatgtt ttattgtctt ctggcccgta
     6601 gtataaaatt ttatgaatat acttagtaag gttttttaaa atcatcacta gctcttcact
     6661 tctcaatttt tttaattcag catcagacag tttgttatta tacggattct tgatgccgta
     6721 attagcaata tttgtcaatc cagctaatat ttttcctttc gatagtttgt cgttgtttcg
     6781 tgcttgtata taatcgttga taaatgcatt taatttattt tggtctggtt gacaactgac
     6841 gaataaatgt tcgagcaatt caacagattt tgcaaaattt ttattcaaac cagttatctg
     6901 aattgtcgtt gtacgtggtt cacattgtgt actgtagtct gttgctaatt tatagaattc
     6961 ttttttcaaa tcttcagacg aatatcggtc agtgtttaaa tagtttaagt attctaatgc
     7021 aattggcaat tttttgatat gattgctgcc catttcgaaa taatagtaaa acgtaaaaag
     7081 ctgattttcc gtattgacaa cactgtacac tgcattttta ttagacaatc cttgttggat
     7141 atcttttttg aaatttgcaa aaactggagt tattggctca actttatttg ctattatcat
     7201 tttcacaaaa tcagattgtg catttcggtt gacttcaact tcgtggatag gtggtttttc
     7261 tactttaatt acactgctat ccattcctgt ttttttatat actacgacat aattattgcc
     7321 aaaccattta ttggcaaatg cttgcacatc ttgtttgtta taacttttta tttcttcata
     7381 aaatgaaagc tcatctttcc agtttacatt tttgataaaa gcatctaata aattgtaagc
     7441 tctgttatta ttgctttcca ttttttgcaa tttctctttt tcaaagttgt tttttacggc
     7501 ttgtaataaa ctctcatcaa aatttccctt cttcagattt tccatttcat gcagcaatag
     7561 acttttaact tcttctaaag tttggccttg cttaggtttg ccttctagca catacatgct
     7621 ataatctttc atgctttgtg cataagatac tgcttccaaa gttttttgtt gttgattgat
     7681 attaatatct atcaaacctg ctgttccatt tgccaaaatt ctgtcgatga attgcaatag
     7741 gccagcttct tttgaattag cacctggcaa tctgtaagcg atatccaccc attcatcttt
     7801 aggaccatat actgtttttt caataggtgc agtaatatct gtttcgatag gtgctttgta
     7861 aggttcaaca ggttttgcta caaatccacc aaatttttca tctacctctt ttattacttt
     7921 atcaggatca aaatcgccag ctaataccat gcacatatta ttgggaacat aatatttttc
     7981 gtagtattta cgaatttctt ttaaagaagg attttttaaa tgttcgatgg ttccaatcgt
     8041 agtttgttgt ccataattat gtgttggaaa taggcttgcc aataaaacct cttgcgcttt
     8101 ccattcgtca ttgtctaaag agcgattttt ttcttcgtac accgcttcta attctgtatg
     8161 gaaaattctg aaaacaggat ttctaaatcg ttctgattct attgttagcc aattgctaat
     8221 ttggttggca ggaatgtcat tgacataagc cgtttgctca taccaagtaa aagcattggt
     8281 gcctttcgct ccaatgatac tcatgagttt atcgtattca ttcgcaatcg catattttgc
     8341 agcaatacca ctggtagagt cgatagcagc ataaattttt gcgcgctcca cttcgtcagt
     8401 agtagaatta tattgctcat ataaatcatc aatttgatct aaatagggtt tttcttttga
     8461 ccaatctaat gagccatatt tatcagtgcc tttaaataac atgtgctcga ggtaatgtgc
     8521 caaaccagtg tgatttgcag ggtctgtatt gctaccagcc ttggtggcaa taatggtctg
     8581 caaacgtggc tcgtctttgt tgacactcat gattacagta aatccgtttt tgagtgtata
     8641 aaatcttgtt tttagtggat cgtcttttac gtattgatag gtgtaatcac ctgtttttgc
     8701 agtgctccac tcatacgctt gttgcgcaaa tccgatagtt gccatactga caacacaaag
     8761 ggaaataaat agttttttca taatgatatt taatgatact aagtaaactt agcgacaata
     8821 aagatattgc tattctgtaa tatgatatat taaatattac ttgctgagaa tttctatctg
     8881 attttctttg ttggctatta tttgtggtga gcctttgtat tcgctaattt tccctttcac
     8941 acatatttct ttgccttgta aatatacttc aggtacataa gaaaagtggc ttctgtcttg
     9001 tccaaaaatc attattgtaa atacatgctc aggatatttt ttatctaaat tgatataggt
     9061 tgggtttgtt tttccgtttt cattgtattt cgtgctgacc actttgccac acacacaagc
     9121 ttgtttgtcg atgtattctt ttacttgtgt ggtgttgatt tttccttttc catagttctt
     9181 actactaatt ttgcttgaac tgtttttgaa ataatttttt tcgaattcag taatgtcgtg
     9241 ttgtgcttct aatgtatttt ctatttcatt ttccattttt ggaaagaaat cgatgccact
     9301 taatttttct atactatcaa tactaacgac atgcttatct aactcatagg gcacattttt
     9361 atttggatag ataaatgcga ttgctttgaa atcaggtgca taataatcga caacaatttt
     9421 aaagatatat tcaggaatag aaactttaaa agaaccttgt ggaatttttg gcaaattatc
     9481 ttgtagtatt ggacctgtaa caacgataag ttcattgttg tcaattgccc attcgcgcac
     9541 ttgcatttct aatttgttcc aggcgccttg attgagttct ttattttgag gaattatgtt
     9601 tgaataaaaa tacgattcac tcaatgcata gcggttccat ttaaaatcag cagaagcagc
     9661 taggtgtcct cggtcaaaac cactgtacca atagtcagca gaatcagaca tattttgttt
     9721 tatagcaggg tctggtctaa aatcattggt gcgcgttgca ctgccataca aaatatcttt
     9781 tggaattaca tgcaccacat aattgggaac acgatgtgaa ggattataac ttgttgtgaa
     9841 tgcagcatgt tttaccaaaa cttcatttgg aatcggagct gcaataccaa cagaatcgat
     9901 tagtttctga atatatttta tcttgttttt ttctaagtct gaaaaaatat ttttttgtat
     9961 ttggtaagat attgtagaat cttggctgta ggaagaagta aactgacaaa tgacaaaagc
    10021 caggaataaa aatatttttc ctgtcattct tgtataagca ggaatcttat taaaaaataa
    10081 aatgagattc cctttttcaa gggaatgact ttcaatttta ttgaaatgta tttttgactt
    10141 catattaatt cttcaaaaat aataaagatt cttcactttg ctcagcacca caattggaat
    10201 aaacaaaaaa cgagccaata aaaattgact cgttttattt tgatatttgt gaattataat
    10261 tctttaatag ctcattcaag aataacgaaa tctagtcatt ataaattgaa tctatttctt
    10321 tgttgtattt ttcattgatg acttttcgtt tcaacttcat cgttggtgtc aactccgtag
    10381 tttcaggtgt ccattctgat tttagtaatt tgaatttttt aatttgctca atatggctaa
    10441 aattaggatt tacttcatca ataatacttt ggtattttgc ttgcactttt ggattggcaa
    10501 tcaattcatc tctagaggcg aatgtgatgt cattttccgt agcaaattgc tctaacacag
    10561 gaaacgctgg aacgattaat gcagaaacaa attttttgct ttctccaatc accataattt
    10621 gttcgataaa gaaattttct ctgaacttgc tttcaatagg tgctggtgcc acatatttgc
    10681 cacctgatgt ttttaataat tctttctttc tgtctgtaat cttcaaaaat ttattgccag
    10741 cattattggt gacaaatgtg cccacatcac ctgtgctaaa ccaaccatct tccgttaaaa
    10801 cttcattggt tttttctact tctttataat aacccatcat gatgtttggc ccttttgcta
    10861 aaatttcacc gtcttctgca attttaattt ctacatcttt gataggcata cctaccgttc
    10921 ctaacatcgc gccaccttct gcaaagcgat tgaatgttaa tacaggcgat gtttctgtta
    10981 agccataacc ttcgcgaatt ggaatacctg ccgcagaaaa cacacgcgcc atttttagtg
    11041 gacaagccgc tgagccagta acaattccca taattctacc acctaatcct tcgcgccatt
    11101 tagagaaaat cagcttatct gcaattttaa atgtaaaaga tggtttttgg tcgtaatcgt
    11161 atttgtcagc catttccaaa gcccagaaaa ataatttctt tttcaagcct gttaatgcca
    11221 atcctttatt gacaatacct tcatagactt tttctaataa acgtggtact gttgtaaaga
    11281 aatgcggtcg aacagttccc aatttatctt ttagtgtgtc gatgtctgaa taatatacat
    11341 ttgcgccagt atgcaaatag gtataaataa ccatgcgttc gaaaatatgg cacaatggta
    11401 agaaactcaa agcgacatga cccgcttcga taggcaactc agcacgtact gtcaaaacat
    11461 tggatacaat attttgatga gaaagcatta cgcctttagg tgtgcccgtt gtgcctgaag
    11521 tataaataag tgtcgccaaa tctttttcat ctaccgtgtc ggagatagct ttgatgtcag
    11581 cgacattgcc accttcacct gcttttatta tttcttcgta gcaagcagca ccttccactt
    11641 ggtcaaaagt ataaatacct tgcaaactgg gaacacttgc ttttaaatcc tttacttttt
    11701 cgtacaattt agcatcagaa acgatacaga tttttgcttc cgcatggttt aagatatatt
    11761 cataatcttt agtgctgata gtaggatata atggcacatt gatggcaccg atttgtccaa
    11821 ttccttggtc gcatatattc cattctgtac ggtttgctga tgttactacg gccactttat
    11881 cgcctttatt gatgccaagt ttcagcaagc ctaagctcat attgttggat tgttgaatca
    11941 attcatcagt gcttagtttc caccattctc cattttttac agtagcaatg gctacctgtt
    12001 gcggatggtt cgctttttga aaatgtaaaa catcaaataa tctttttact tgcatgactg
    12061 gtttataatt tatggtaaaa tgaatgaatt aagtctttgt atttctcggt tattttcttt
    12121 ctttttaatt tcatggtggc ggtcagcata ttgttttcga cagtccattc gtctgccagc
    12181 aattcaaaat gcttgatttg ctccactttg ccaaagttaa catttaattc tgtaataatt
    12241 ctcttatata attctttaat ctgttggttt tctattaatt cttcattaga taatgcttta
    12301 atttcgtttt ttgccgccca ttctcttaaa ttctcgaaat ttggaataat taatgcagaa
    12361 atgaattttt caccatcgcc ttgtagggca atttgttcaa tatagaaaga ttctttgagc
    12421 ttgttttcta tgggcgacgg cgccacatac ttgccaccag aggtcttaaa aagctctttt
    12481 ttgcggtctg ttatttttaa atatccatca ctcgtaaatt cgccaatatc acctgtgagc
    12541 aaccagcctt cgttgttgaa cgatttttta gtttcttctt catttttata atagcctagc
    12601 atcacatttg gagcttttat caaaatttca ccatcttcag caattttaat ttgaacactc
    12661 gacaataatt taccgacaca gcctgctctg aattcttgct gacgaaatgg cacaacgctg
    12721 actacaggcg aacattctgt gaggccataa ccttcaagta tcggaatgcc tgcattggta
    12781 aaaattgttg ccaacctcgc ttgcaagggc gcagaaccac aaataattgc tttaatattg
    12841 cctcccagtg cttcgcgcca tttattaaaa acaagcttgt tggcaatata taaattcgtt
    12901 gattctgcta atgtcctcgt tttgcctaat tctgtatttt gcgcttgtgc caccgaccaa
    12961 aaaaatagat ttttcttaaa accatttagt gtttgtcctt tcttgattat tttttcaaaa
    13021 acacgctcta atagccttgg tactgtagtg aagtaattag gcttgatatc ttgtaaatgc
    13081 tcagaaatag tttccaatcc atcagcgaaa taaatggaaa cacttcccgc aaaatagaca
    13141 taattgatgg tacgctcgaa tacatgactt aaaggcaaga agcttaatac gacttctcct
    13201 ttgccgacag gaatagcttt aagacaatct tttacattgg acacgatgtt gtaatgagat
    13261 agcatcacgc ctttaggtat gccagttgta cctgatgtat aaatgattgt cgctaaatcg
    13321 ctagcttgga tactttgttt tatggtgttt actttctcca ccagtatttt gtcattagtc
    13381 aataatgttt tccaattcgt tacattgtca acgacatcaa aactaaaaac tccttttagg
    13441 ctgggaacta gcggaataat ctgtgctatt tttcggtaca tcagcttgtc tgagacgaac
    13501 acgtatttca cttcggcatg attaaagata aattcgtatt ctttgctgct gatagttgga
    13561 tatactggaa tatctactac gccaatttgc atacaggcta agtcagtaaa atgccattct
    13621 ggtctgttgc tagtagaaat gatagcaaca gtatcgcctt tttgtaagcc taattgcagt
    13681 aagccttggc tgagttgatt ggctgtttca ataaatgtct gcgtggagta gtgagtccac
    13741 gtttttttgt cttccctacg cgacagacat ttctctaagg gactgtttat ttgctgatgc
    13801 gcaatgatat caaatattcg actatatggc acgctgttgt attaaagtga atttcaaatt
    13861 tataaaataa attccgttga acaacggctt attctaaaaa gttcttattt caagcaaaag
    13921 tggtgtacac ctttctgttt tatttgtaaa aaccaaataa gacttcgtaa cataatcttg
    13981 tgaatttcca tttttaaaaa ttatattcgt tgtgctcatt tttatggaaa aaaaatcgtc
    14041 attaattatt tataaagcat cggcaggctc tggcaaaacc tatgccttag caaaagaata
    14101 tcttaaaata gtaatacaaa atccttatga ttataagaga atattagccg taacatttac
    14161 gaagaaggcg acggcagaaa tgaaatcgcg aattgtagaa tatctaattt tattagaaaa
    14221 gaaaagcgat acagtagata cgttgcgcaa ctccattata gacgaaatta aagaagcaaa
    14281 tggcattgat gtttctgctg tgtttgacaa gcatactcgc atcgcactgc aacttatttt
    14341 gcatgactat tcgcatttta atgtctctac catagatagc ttttttcaaa gtatcatacg
    14401 tgcttacgct aaagagttag atttacccat tggcatggaa gtggaattag atacagcatc
    14461 ggttattgaa gaagcaatac aagctgtgtt gaaagaatat aaaacggata atgatacgtt
    14521 ctccagttgg atagaagcct atatttttga gaaaatagat gacgacaaag gttggaatat
    14581 cgagaaaaac attgcatcat tagccaagga attgctcaat gaagactatc gattgctgac
    14641 ggaagacagg caacagtctt tcagcattga caactataaa gaaacacgtt tggctttgaa
    14701 aaaaattgtt gcaaattatc gtcaagccat ggatgatttg gtgcaaaatt ttgaacaaga
    14761 aatagagcgg caagccatcg acttaagatt atttaaaggc aaaagcactt ttgtttcttt
    14821 tgtgtcaaaa attaaaaatg acgcgccaga atttacagat accattctga atatgatggc
    14881 gagcgacgaa gtgttatcta aagaaactgc aaaagatagt gtccttaaaa taacaatgga
    14941 aaccgcatgg cataacacgg taaagccatt tttattagcg atagccaatt taatagaaaa
    15001 caagagtaag caatatcatg cagcgattat tgttctgaaa aatatttatt cgcttgcgct
    15061 tctcgaaatg ataaatgaga aaataaaagc ctataaacaa gaaaataatc tcattttaat
    15121 ttctgatact aaccagattg taagcttaat agctcgacat gaagatgtac cttttatatt
    15181 tgaaaaatca gccagttttt tgaagtatat tttaattgac gaatttcaag atacatcgac
    15241 tttgcaatgg catggcttat tgccattatt attagagatt ttacaatcaa ctgaaggatt
    15301 ggtattaata gttggcgatc caaaacaaag tatttacaga tggcgtggtg gaaaaatgga
    15361 attgatgata gatggcattt catcagcatt tactgactat ttagaaccaa aaaatcagaa
    15421 gacactaagt gtgaattaca gaagtgcaca agaaattgta gattttaata atgcattttt
    15481 tagcacctta aaaaatataa ttccactaga caatccgtac tttgagcgcg tactcgccga
    15541 cgtagagcaa aaagcaaaaa aaacaaaaat taaaggatat gtgcgctgtc aatggcttga
    15601 tcccatcaaa ggcgaagatg tgtgccttcc tgctatttta gcaattatta aagaagttac
    15661 tgcatataaa aaacatagtg atattgccat tttagtgcgc acaaacattc aaggaaatgt
    15721 ggtcgcaaat tatttacaag agcaaggtgt tccggtgatg tctgctgaat cattactgct
    15781 caatggccaa gcaaatatag ctttattgct gtcggcatta acctatatat tgcatagtga
    15841 tgaagatttt tatgtaattc aatttaatta tttgctcgca cagtatttac aaaaaccttc
    15901 cttggaaaat tatttacaga aacaaaataa acagtatttt tttgaaaatg aatttccatt
    15961 atttcaaaga aaaaatactt caacattgcg tagtatttct attaacgaac ttgtgtttca
    16021 ggtagcgcaa gcattgcaat ttgatacgca actcgatgag tatttgttaa ggtttcaaga
    16081 tgttgcccat caatttgcac aacagcgtag cacatcgtta agcgatttta ttaattattg
    16141 gaacgagaat gaagataaat tatccatatt gccaccagaa ggtattgatg cagtaaatat
    16201 tatgaccatt cataagtcaa aagggctgca atttcctatt gtgattatgc catatgcaaa
    16261 tcaggaatta aaaccaaaat cggaaagttt gatttggata aatagcgatg aaaaaccatt
    16321 taataagttg aatgcatttc cagtaaatct tacatctaaa acaaaagaca gcctttttga
    16381 agcggaatat ttgaaagaat ttagtgccac ttatttagac aatattaata ttctatatgt
    16441 agcctttaca agagcagaag agcaactata tgtcttagca gatgcatcga agagtgatag
    16501 taaagacaaa tcgccactgc caaatttcat gaataaattg atacataaag tggtgcaatc
    16561 aatgcactta gaaaatgcac aatttgaagc aaattgtttt acttatggta ctgtcaatac
    16621 aaacaaacaa gctccagaag aaataaacac aacggcttat ctatcaccac aacattttac
    16681 agattttaaa acgaaagtac ctttagctat tacgacatca tataatgaag cacaagaaaa
    16741 aggcacgaaa atgcatgaag tactagcaaa aattaaacaa aaaaatcaat ggaaaaatgc
    16801 gatagcttca accgtgacag atgatgatgg ctattacgcg caacagacac aactagtgtt
    16861 gcagtttttt gaaaaacaac aatggtttga tgaaaaatgg caacatatca atgagcgcag
    16921 ttttacattt aatggtgaat ttatgcgcgc agataaaatt ctgttatcag cggatagttg
    16981 tattattatc gactataaaa ctggcgcaaa agaaaataca catatacatc aattgcaaaa
    17041 ttacaaagct gcttacgcta catttgtgcc acaaaaagtg agcgcctttt tactttatat
    17101 cgatacctta gaacttcaag aaatataaaa agcaaaatgc atacaatcca tcaacaatac
    17161 gaaaatattt ttgaaattat tacacttgct aatgtgcatc atacggccaa agttgtagta
    17221 aatattggta attccttgtt ttcctacaca aaaaatagac ttgaatattt acatttccct
    17281 tttgatttac aagaatatgc caacaacgat aaattagcag gaaatccatt tatgcatcct
    17341 tgggcaaacc gtttagaaag taattgcata cgtgttgaaa atttacaata caactttcct
    17401 atacaacagc aacatctatt gtacagagat ggaaaccaat tgccattaca tggtttattg
    17461 ttgaagtcaa ataaatggaa aacgattaca acacaagctt cagcatcgca atgcaagcac
    17521 gtcgcagcgt atatatttga tggtgaagat gaattatcca ttttcccatt tttacacaat
    17581 attcagatga ctcatatttt gcgtgatgat acgctacata tcgaaacagt gattcataat
    17641 ctggataaaa aagatatgcc aattagcttt ggctttcatc cgtattttaa aaagttagat
    17701 gcgcatgcaa aattacattt agcagcatcc aataagattg aaaccaatag cttgatgatt
    17761 ccaacaggcc aggtgacagc gaaagaaaaa tgctggaatt ttatcaatca tgaaattgcg
    17821 attgatgatg taatgttcga tgatggattt caagatttat tatttgaaaa tggaatggca
    17881 gatttttctt tggacaatat acatgttctg ttttgcgatc aatatccatt tgcacaagtg
    17941 tattcgccaa gcaccataga taagccttat gtttgtattg aaccaatgac agcacctaca
    18001 aatgcgctga attcaaatgc ctgccaactg ttgaagtcaa atgaaacttt ttctgcttct
    18061 ttttctataa aaatagcgta gattgtttgg agtactgttt tattgaaaaa catatctcgc
    18121 ttaatcatgt tttattaaaa aaaatcaatg tcagtaaagt aaacagcata gcaattaggc
    18181 acatattttg attatcttcg ttacattaaa tattggtatg aacaaatttc tattaacaac
    18241 tgcatttttc ttcactgctt tttttgcagt acatgcacaa atgcctgcaa ttggcacaaa
    18301 agcgacagaa ttaaattata aagatccatc tggcaaagaa atcgcattgt catcattaaa
    18361 aggtaatatg gttttgctcg atttttgggc atcttggtgt ggtccgtgcc gtagaaacaa
    18421 tcctgcatta gtggccttgt actctaaatt tcaaaataaa aaatgggtaa aaggcgtaaa
    18481 aggctttact gtgtatagcc tttccttaga tcaaaatgca gaaaattgga aagctgccat
    18541 tcagcaagat ggcctagtat ggccttacca tgtttcagat ctaaaaggct ggaatgcaga
    18601 aggagcagca aaatatggta ttcgctctat tccgcaaaca gtattaatcg acgaaacagg
    18661 atatgtgatt gcgattaatc catcacataa tttagtcgat caattagtaa gcaaaaaact
    18721 gagcagcgga acgactccta gcaaaaccaa atcgaaaacc aaatcgtcaa aatcaatcaa
    18781 aaaataagtg ccaaattgac gtaaaatgca attggcaaac aatttgactt agattgttaa
    18841 aatcttagtt atgatagagg aaaaagagac tatagttgat gcgcaaacag aacagcaggt
    18901 cgtcgaggaa aataaaaatc agtcagaaag tcagcaagaa tcaaaaaatg ctgcaaatga
    18961 gtttgccgca gaggacgaat tgtcggcttt taaaaaagaa atagaagagc aaaaagacaa
    19021 atatttaaga ctatttgcag aatttgacaa tttcaaaaaa cgcaatgcca aagaacgttt
    19081 ggagttgttt aaaacagcag gtcaagatat tgtacgcgaa ctattacccg tattagatga
    19141 ttttaacaga gcagacaaag tttatcaaca agataataat gcagaaaatt ttgtcgttgg
    19201 attgaaatta attcaagaaa aatttcataa aactttacaa aacaaagggc ttaaagcaat
    19261 tgatagcata ggtaaagatt ttgatgtggc acagcacgaa gccatcgcag aagtgcctgc
    19321 gccaacacca gagctagcag gaaaaatttt ggacgaagta gaaacaggct acaccttgaa
    19381 cgatacaatt attcgatatg caaaagtggt tgtaggtaaa taaaaataga aacgattaga
    19441 gagttttaac aactgcgaag cagaaaacaa caagcaataa caatggcaaa aagagattat
    19501 tacgaagtat taggtgtagc aaaaggtgcc agtgccgatg atataaaaaa atcataccgc
    19561 aaattagcat tacaatatca tccagataga aatcctggtg acaaagtagc ggaagaaaaa
    19621 ttcaaagaag cagcagaagc atatgatata ttaagtaatg atgagaaacg cgcgcgctat
    19681 gaccgatttg gacatcaagg tgctgctggt ggttttggtg gaggtcaagg tatgaatatg
    19741 gatgatatct tctctcaatt tggcgatatc tttggcggtg gcggtgatgg caatccattt
    19801 gagtcatttt ttggtggcgg tggcagaaga aaagagcgtg gtcgcggaca acgtggaagc
    19861 aatttgcgtg ttaaagtaaa aatggattta gaagaaattg caaatggcgc acagaaaaaa
    19921 attaaagtaa aaaaacatat cgtttgcgat acttgctcag gcattggcgc aaaagatagt
    19981 tcttcctatc aaacttgtaa tacctgcaat ggcagtggtg tggtgcgcaa agtgacacaa
    20041 acttttctgg gtcagatggc aacaacaggc acttgcccaa cgtgtgaagg ggaaggaaaa
    20101 attattacca ataaatgtac taaatgccga ggcgaaggtc gtgtgtatgg cgaagaaatt
    20161 gtgagtatcg atatacctgc tggtgtaagc gaaggtattc aactctcgat gaatggtaaa
    20221 ggaaatgctg gcatacgagg aggttatcct ggtgatttat taatcaatat cgaagaaaca
    20281 ccacatcctg agctgaaacg cgaagacacg aatattattt atcatttata cctgaatttt
    20341 gcagaagcag cattaggcac acaagcagaa gtaccaacaa ttggtggaag cgcaaaaata
    20401 aaaattccaa aaggaacaca aagtggcaag cttttccgtt tgcaaggcaa aggaattcca
    20461 agcttaaatg gttatggaaa aggcgaccaa atagtagaag tgcaagtgtt tacgccacaa
    20521 gatttaacga cagaagaaac agcattgttg gagaaattaa gagattcaaa aaatttcaat
    20581 ccacaagtac aaaaagaaaa aaataaaggc ttcttcgata agttttttca ttaataaaac
    20641 atagtgtcaa aaattctgtt gtaataaatt atctcacaag gatacttata tttacacatt
    20701 aaatttttca atttgtattt gttagatgta caacatatct caaagacgtt tcagcaaaat
    20761 gtagcactac aagacgtgtc tttgtcgata gacaagcata aaatttatgg tttgctcggg
    20821 ccaaatggtg caggaaaaac cacattaata cgaacaataa cacaaatctt ttttccagac
    20881 agtggccatc tttttttcaa tggagaacct ttagcagaaa aacaccaaaa tctgattgga
    20941 tacatgcctg aagaacgtgg cttgtataaa aaaatgaaag taggtgagca gttgatgtat
    21001 tttgcgcaat tgcgcaattt caccaaagca caagcaacag aaaaaatcaa cttttggcta
    21061 aaaaaattag acatagaaag ctggcgcaat aaaaccattg aagaactgtc aaaaggaatg
    21121 cagcaaaaag tacaattcgt cgctacggta ctacacaatc caaccttatt gatattagat
    21181 gagccattct ctggcctcga tccaattaat gcagatctta taaaaaatga aatatttgaa
    21241 ttgcaacaag ccggcactac tattatcttc tcaacacata gaatggaaaa tgtagaagaa
    21301 atttgtaaag atattttcct aattaataaa ggtaagatat tgttgaatgg aaatgtacaa
    21361 gcgataaaac aagaatacaa aaaacattta ataagcgttg agacagcaaa caaggtagtg
    21421 ttgaacgatg cagtgcgttc cgcattgcac gtacacgaac aaagtgataa aagaattgtg
    21481 tttaaattga cagaaaatca aacgcctaat gatatgctaa aactcttatt gcaacaacaa
    21541 atagaaattg tagcgatggc tgaaattttg ccaacagtac acgagatttt tattcaaacg
    21601 gtgaaagaaa attcttaaat aaaaaaggaa atatagttcg ctaaaatagc atcattaata
    21661 agcacaacac caataataat gaataaagtc tggtacatca tacaaagaga atatcttacg
    21721 cgagtaagga aaaaatcttt cattatactc acgcttttgt tgcctgtgtt gattggtttg
    21781 atgagcgttg cgctgattga aattgcaagt aatgctcaag aagagttgac cattgcagtt
    21841 aaagatgaca atgcatggtt tgaggataag tttagaggta tggacattgg cgacaaccta
    21901 cggtttaaat accttaccaa caatccacag ccaatagatt ccttgaaaca tctgtatcaa
    21961 aatattgact gcgatggcat tttgtatatt ccaaaattag accttaatca gccacgtggt
    22021 attgcttact attctgaaaa cgaattaggc atggcaacag aaaaatatat tacagaaaaa
    22081 ttatctgaag aaataagaaa gcgtgcactt tcgcaacgga attataatat cgacactatc
    22141 gaacatataa cgcaagcaat cgacatcgaa aaaattatag acaataagaa gcagagcgga
    22201 ggctcagaaa ttgctggtgt tttcggctat gcttgcggtt ttctgattta catatttcta
    22261 attgtatatg gcgcaatggt gatgaaaagc gtgaccgaag aaaaaacaaa tcgcatcgtc
    22321 gaagtattgg ttacaacagt gaagccattt caattaatga ttggaaaaat tattggcatt
    22381 ggtgcagttg gccttacaca atttttgctt tggggaattt taacttttgg cattaatcta
    22441 ttattgccat tttttttagg cgataagatg atggcaatgc aaagcatgca gcaaaccaat
    22501 gctatggcag tatcaacaga ggaaagtaca caacttttga tgaatatttc taatgcattt
    22561 caaactttag agataggcag aataatcttt agtttcttgt tttattttat ttttggctat
    22621 ttattttatg cgtcgcagtt tgccgctatt ggtgctgcta tgactgacga cagcgacgca
    22681 cagtcattta catttccgat taccattcca attatcatat caatggtgct catgtcagtt
    22741 accttatctc agccgttttc atcagccgca ttttggggtt cgatgttgcc attctcttca
    22801 ccaattatta tgattacacg gataccattt catgtgccgt ggtgggaaca aatattgtcg
    22861 atgtttatct tgtttttatc ggcaatggca atggtatatg ttgccgcaaa aatttataga
    22921 gtaggcattt taatacaagg caagaaagtg tcttttaaag aaattagcaa gtggattttt
    22981 tattgagtaa atgctgcgtc agacaatagt catgtgcagg aaaacttgtg tatttcttct
    23041 tggtgttcct atatttagtt atgccttaat aatcaaatat aaaattacaa ttgcagtacc
    23101 tgcattttct tttcccagtt ccaagcgctc agcatggcat cgtctaagga atattgacat
    23161 tgccagccaa gttgcgttgt cgcatttgta ttatcagcat acacagctgc tacatcgcca
    23221 gcacgacgct cacctattac ataatttaat ttttgcaggc tcactttttc aaaagcatca
    23281 attgcttctt ttacagaaac accaatgcct gaacctaaat taaacacttc acaattacta
    23341 tcgtttctct tgttaactaa atattgtaat gccaaagtgt gagcatgtgc aatgtcacac
    23401 acgtgtatat aatcacgaat gcaactacca tctcgagtat tgtaatcatt gccatacacg
    23461 caaagctgtt ctaattttcc gatggcagtt tgtgtaatgt atggcactaa attattgggt
    23521 ttgtcgatag gtatttcacc aatatgcgct gatggatgcg cacctattgg attaaaatag
    23581 cgcaataaaa tggaagcttg tgcaggatat ctttttgaaa aattgcgaat aatatcttca
    23641 ccgatttgtt tggtgtgtgc gtatggactt tccgcatttt ttataggcgt attttccgtt
    23701 actggcaaaa catcagcatt gccatacaca gaacaagaag aggaaaaaac aaaatttgga
    23761 atgcctaatt tttctatgct ttctaaaata ttgactaatg actcaaggtt gtttttgtag
    23821 tataataaag gcgctgccac cgactcaggc actgacttta atgccgcaaa atggataatg
    23881 ccaacggcgt cttgatgtgc tgataataca tcatgcgtgg cattagcatc acatagatca
    23941 atgcgataaa atggaatggt tttacccgtt attttttcaa tgcctgttaa tgcgtatgtt
    24001 gatgagcgag ataaattatc aatacatacg acatcaaatc catgttgaat taaatctaca
    24061 attgtatgtg agccaatata acctgtgcca cctgtaacta ttactttcat tctctagctg
    24121 atgttttttg aaataagtat ataaaattat tcatttaaaa tgtctaataa atattgacca
    24181 taaccacttt ttagcaaagg ctgtgcgatg gcctgcaatt cttctttaga aataaatcct
    24241 tttctatatg ctacctcttc gatgcaacct acttttaagc cttgtcgctg ctctattact
    24301 tgcacaaatt gtgatgcttg catgagcgaa tcaaaagtgc ccgtgtctaa ccatgcggtg
    24361 cctctgtcta atatgcctac ttttaatttt ccgcgttcta aatatatttt gttgacatca
    24421 gtaatctcat attcgccacg tgcacttggt tgcaaatttt ctgcaataga caccacttca
    24481 ttgtcataaa aatataaacc aggaacggca tagtttgatt ttggatgcag tggtttttct
    24541 tctattgaaa tggcattaaa ctttttatca aacgctacaa cgccatatct ttccggatca
    24601 gaaacgtgat aagcgtaaat cacgccgcca tcagggtcat tatttttttc taataaatcg
    24661 cctaagcctg agccataaaa aatattatca cctaacacta aagccacttt gtcatttcct
    24721 ataaaagact tgccaatcac aaatgcttgt gctaagccat ttggcacttc ttgaatggca
    24781 tattggaaat ggcaaccata ctttttgcca tcgcctaaga gtctttgaaa attaggcatg
    24841 tcttgcggtg ttgaaataat taatatttcg tgaatgcctg cctgcatcaa taccgacaac
    24901 ggataataaa tcatcggttt gtcgtaaata ggcataattt gtttgcttat tgcaaaggtg
    24961 ataggataga gccttgttcc tgagccacca gccaaaataa ttcctttcat agcggtaaag
    25021 taatcagaaa aatcaaattg ttaaaaataa ttgacaaaaa tacatagtaa aacaagataa
    25081 aattaatatg tttgcctaga aaattggaag atttagaaaa ttgtgttaat ttagcgttaa
    25141 cataaataaa atatccgcaa ataatggctg aaaaaacatt taaaattctg gtagtcgatg
    25201 atgagccaga tatcttggaa ttcttgagtt acaatttgga gaaagaaggt tttgtagtag
    25261 aaaccgccga aaatggtaaa caagcattag agaaagcaaa gaaaaaccaa ccagacatcg
    25321 tactgctcga cgtgatgatg cctgaaatgg acggtattga agcttgcaga acgatgcgcg
    25381 aaatgccaca gttcgagcat accattatcg cctttttaac ggcacgaacg gaagattatt
    25441 ctcaaattgc tggttttgaa acaggtgcag atgactacat cagcaagcca ataaaaccaa
    25501 gagttttagt aagtcgctta aaagcgcttc taagaagata cgaagctaaa gaatcaaaat
    25561 catctttctt agatgttggg aatattcaaa ttgatagaga acgttattta attattttca
    25621 acggtaaaga aatggctgtt cctcgtaaag aatttgaatt gctatatcta ttggcttcta
    25681 agcctggcaa agtattcaaa cgcgacgaaa tcttgaatga aatttggggt cgcgatatta
    25741 tcgtaggcga tagaacgata gatgtgcata tcagaaaatt aagagaaaaa ttaggagaag
    25801 atttaatcaa aacagtaaaa ggtatcggat acaaatttga agaaatatga gaagatccgc
    25861 ttctccaaat ataatatcac ttttagcaag catggcagta gcaatttttt ctactgccat
    25921 ctttttattt attactgcca tctttcactt aaaaataaat tatagcgttt acatcatcaa
    25981 tatcttcatt attttcttag taacattact gctgtttcgc tttgttttag tccggtttat
    26041 ttatagcaaa ataaaaatta tttataaaac gataggcaaa ccgctgaaat ttcagaatga
    26101 agttaaagaa ggtagcaaaa atgtattgaa aactgtagaa cgcgatgtgg cagaatgggc
    26161 aattaataaa aacaaacaaa tacgcgagct gcgcaagatg gaacagtatc gcaaagaatt
    26221 tgttggcaat gtgtcgcacg aactgaaaac acctattttt aatgcgcaag gatatattga
    26281 aactgtctta gatagcgact tagacgatcc tgcttttatc aaacaatatt tagagaaagc
    26341 caatagcaat atcgaacgct tagaaaccat tgtaacggat ttactcgaaa tttcaaagtt
    26401 tgaaagtggt agaatagaac taaataaaga agcattcgac ttggtgaaac tgataaagaa
    26461 agtttttttt cagtatcagc acattgctca gcaacaaaaa atttctttaa aatgtcatag
    26521 cgaggaaaat gctgtctttg tctttggcga ttcaaaacgc attatgcagg tgctggaaaa
    26581 tttaatttcc aattctataa aatatggtaa ggaaaacgga catacagata tttattttca
    26641 cgatttagaa gatcaatatt tagtagaaat cgttgatgat ggtccaggta tctcgtcgga
    26701 acatttgcca agattattcg agcgttttta tcgtgccgat aaaagtagaa atagaaaaat
    26761 aaacggaact ggtttgggtt tgtctattgt gaaaaatatt atcgaagcac atgaacaaac
    26821 tatcaatgta agaagtgaat tagaaaatgg tactacattt agctttaccc tgcaaaaata
    26881 tattcctgaa taattaatat tcgcaatagc gcattacttt cgttttataa agcacttttt
    26941 ttgctgcatc tttaattaaa acaatattta cacctaaaac aaaatctcga ttttgcaaat
    27001 aaataatttc atttgattta ttgtaattaa tttgcttaga tgaaattaat cgaccgctga
    27061 tatctataat ttctatcgtt aataattcat ctttctcatg tcgaatatta agtttaggtg
    27121 ctatctgaca tgagatagga tttggagtaa tgctaataaa atcagaacct acagcgatgc
    27181 tatctgtttt acaccaataa aaagtttcaa aatttctgca ttcactaatt ggacgtgcat
    27241 tgatggaatc gctggcaata cttgtgttgg tttcgatagt gccatttgta atattattaa
    27301 taaaatcaaa gcttttatta ttgatataat ttattgcatc agcatcgcca cctaatggtg
    27361 ctgacaagga gttaaatcca tgattgtaac cacagaaaac atgaaattga taaggtacat
    27421 tttgctgttc tagaataggt gtgaatatac ctggcccata agaccgaatg gtgttgctac
    27481 aattgcctaa ccagccatta aaaaaatcaa ctgcaccatc acaactatta ttaaacaata
    27541 atgtaggata aggatgaacg atgtttacat catttttata gaaaccacct acaatactaa
    27601 tgattccagc tatttctgtt gaatttctat aagcatttcc ttgcagatct aagctgccta
    27661 atcgtgataa tcgctcttgc gtaattaata catcttgcat atataaatga tgcaacacca
    27721 aaaaaccacc agaactaatg cccattaaaa atattttatt tgagtcgatg cctaaagtgt
    27781 ttgcattggc tttgcaaaat cgtataagcg cacgattgtc ttgataggca cgatgcgcag
    27841 catcttcaaa atattcaggt gttacacttt cgcataaagg tttcgatgtg gattcgtacc
    27901 agccttgtct aaaatcaggc agaataacgc tgatattttt ggacgtaaaa tagtctgcaa
    27961 atatttttaa tgcggcaaga ttcttatcac cagcagcata accgccacca tggtaaacaa
    28021 taagcactgg ccgctgtgtt gagaataaat ttggtttata taaatcggct tttattgctt
    28081 cttcctgccc tgcaaaattc gtattgttgg cataggtgaa agtagatttt acactcgctt
    28141 gtataaaagt gatatttaac aaaaacatac ttattacaaa gatgatatgc gtaaatagtt
    28201 tacgcatatt tctcgataat tttatcgcac gctttatcta gcatgtcata tacattttga
    28261 aatccattgt catcaaaata aggatcgggc acactcctat tttcgccagg atacacttcg
    28321 tttaaaatta atttgatttt actttcttct tttttgtcta aagcccagcc taataaatct
    28381 ttatagttgg ccgtatccat agcaaatatc aaatcgaatt cttcatagtc ggcgctatgt
    28441 acgctgcgtg cacgttgtcc agaaatgtcg ataccgtgct tgttagctat ttgcacagaa
    28501 cgatagtctg gaccaaagcc aatatgccat tttccagtgc ctgcactatc aatataccag
    28561 tctagttttt gctgagcaca cttatggcgt aagatgcctt ctgctaatgg agagcgacag
    28621 atatttccta aacaaaccat caaaactttc atattctatt tttagaagga tattattaat
    28681 aaaaacaaaa atgattgttt tatcttacaa tgcaaaatga aacggtttat tttattgcta
    28741 tttataatgt ttccaatgtt ttcttcgaca aatgcaaaaa agcattggca aaaaacagct
    28801 agtggcattt gctataaatt atatacaaaa gataaaagcc aagtgagacc taaaattggc
    28861 gattacattt ggatgcattt agttaaatat tcaccaacaa aaaaggagat ttttaataca
    28921 cgtattttcg atgtaggaaa tggtgtagaa atgcaattga aagctgtgca aaaacaagtc
    28981 gatgtgaccg aaatttttac acacatgaga aaaggtgatt ctgccttggt caaaattccg
    29041 tgtcatttgt tggatagtaa tggaagcaaa aaaaagcatt atacttttcg tttgcagctc
    29101 attgattttt ctaccaaaga agcttatgaa aaggtacagt gggaaaaaga atttctacaa
    29161 gcacaggcag attctttggt gatgatagaa tacttaagta tgcacaatat gttagatgct
    29221 ttgaaagatg cagcaggcaa ttattttgta aggcaaatac gtggaaatgg agcgcagttg
    29281 caaattggcg atagcgtaca attgcattat attggaaagc tgaccacaga ttttgaattt
    29341 gataactcgt atggccgaaa gcaaacactt gcttttgtgg taggcaagca gcaggtaata
    29401 gaaggtttag ataaaggtat atgcaatttt tattatggcg ataaaggtat attgctcatt
    29461 ccaagtagat tggcgtatgg cgataagtca gtaggcaaaa taccagcaaa tgcagtactc
    29521 gtatttgaca ttgatatttt acctaaataa aaaaagtgtc gcacatagcg acacttttca
    29581 atagcttttt tagataatta tgcaattgtt tctacaacag ttttctcgga tttttttcct
    29641 tgtatttttt cttttattct atcaaaaatg taataaacag taggaacgac cactaacgtt
    29701 agcaataatg aactgcttag tccaccaatg attacccatg ccatagaacg tttaaattct
    29761 gcacccggtc catttgataa tgcaatagga agcataccaa aaatcatagc aacagtagtc
    29821 attaagattg gacgcaaacg ttcttttccg gcctctatta atgcttcttt taatggcaag
    29881 ccttgtgctt ttaaatgatt ggtaaagtcc actaataaaa taccattttt tgccaccaaa
    29941 cccatgagca tgataaagcc aatgataccg aaaatactta aactctcgtg tgttaacgcc
    30001 aaggctaaca ccgctccaat taaagcaaca ggaatagaga ataaaaccac aaatggatat
    30061 aaagtactct catatagtgc gaccataatt aaatatacca acaaaatacc taagcccata
    30121 gcgattccta agctgccgaa agaatcacct tgattttttt gctcaccaag gtattctatt
    30181 tttacgctag atgggaaacc cgctttgtcg atttgttctt tg
//
LOCUS       DAOQQV010000018        29311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_461_length_29311_cov_7.836649, whole genome shotgun sequence.
ACCESSION   DAOQQV010000018 DAOQQV010000000
VERSION     DAOQQV010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 29311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29311
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_461_length_29311_cov_7.836649"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLL59_03495"
     CDS             complement(<1..208)
                     /locus_tag="PLL59_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22899.1"
                     /translation="MQFQILATKRVAIASAMVLMSGEKVKELGVQPLAKIVSFADASQ
                     DPEWFTTTPAKAIPRALHKANLNVE"
     gene            203..433
                     /locus_tag="PLL59_03500"
     CDS             203..433
                     /locus_tag="PLL59_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMY22900.1"
                     /translation="MQENSFLQYTDVFEDPNMPDVMVTSVYLKQVACGTELKITQENI
                     PAAIPVEMCYLGWQESLEKLKKLVEPNIPDGI"
     gene            complement(581..2380)
                     /locus_tag="PLL59_03505"
     CDS             complement(581..2380)
                     /locus_tag="PLL59_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY22901.1"
                     /translation="MSETLTEVKNLLNGGEFLVKSSNFQDVFTPEDLNEEQLMIVDTV
                     KSFVETEITPIYDRIEHQEPGLTESLLAKAGELGLLGLAIPEEYGGMGKDFNTNSLLV
                     ENLAKSRSFSLSLGAHIGIGTLPIVYFGNEQQKTHYLPKLASGELKACYCLTEPSSGS
                     DALGAKTVAILNEEGTHYIVNGQKMWITNSGFADVFIVFCQIGGDKFSCLILEKDMPG
                     LTLGAEEKKMGIKGSSTRQVFLENVKVPKENLLGEIGKGHLIAFNILNIGRYKLAAGV
                     LGGSKSALEQSLKYAEERQQFKTPISNFGAIQYKLAEMTTRIYALQSACYRASDMIDN
                     KEKELVAQGKPYQEAIMMAAEEYAIECSLLKFIGSEVLDYCVDENVQIHGGMGYSEEL
                     AAAGAYRDARINRIFEGTNEINRLLSVDMLLKRAMSGKIDMMTPALAVQKELMSVPDF
                     SANEDNEPLAAELKAVQNAKKAILMTAGAAVQKLMKQLKNEQEILMNIADMLAEVYAL
                     ESTVLRTQKLIANNGEAAVAVQIDMTKIAISDGMERINLYGKHALQAFGEGDELRIML
                     MGLKRYTKYDIVNTKEARRRVAAKVLEANTYCF"
     gene            2619..>3238
                     /locus_tag="PLL59_03510"
     CDS             2619..>3238
                     /locus_tag="PLL59_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HMY22902.1"
                     /translation="MASYKRIPLKPFPNGWYVLAHSHEVKNGEIITRRFAGKDILLFR
                     TASGKLSVSDPYCPHMGGHLGYGGVVEGDSIKCPFHHFCFDTKGDCTATGYGSKPSSK
                     LKLPVYHFDEKNDLILIWFDEKNAAPTWHVPQEDWSEWTAVRFAEYDFNSHPQETSEN
                     SVDVGHFGIVHGYTGVETLHEMESHEHYLYAKYAMNRVADFVGKKRT"
     assembly_gap    3239..3338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3542..4144
                     /locus_tag="PLL59_03515"
     CDS             3542..4144
                     /locus_tag="PLL59_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22903.1"
                     /translation="MKNYVFLLLSLTLLTTSTFAQENWKLEKDKNGIKVWNRKVAGTA
                     IKEFKVSTIINTSTEKMLAFLKNTAKYDQWMYKVDEGSVKVLKRVSDNDYYTYMTISA
                     PFIKTRETITHMTFNSQDAKGAILINLDAAPNLLPLNDKYVRIPKMKAYFKIVPLGNG
                     QIELIHQALGSSGGNIPEALINLTSVDCPFYMFTKIKSMI"
     gene            complement(4128..5375)
                     /locus_tag="PLL59_03520"
     CDS             complement(4128..5375)
                     /locus_tag="PLL59_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HMY22904.1"
                     /translation="MSLIYKITKPSSDCIQGEVQLNGSKSISNRVLIIDALCNHQLQL
                     ENISNADDTVFLQQILQSNDAVLDAGAGGTTFRFLTAYLSTQEGREVVLTGSERMQQR
                     PIKILVDALLQLGADISYVNKEGFPPLRIRGKKLKGGKISLPADTSSQYITALLLIAP
                     TLQEGLTLELIGKIVSVPYIKMTLKIMEYFGVKSTFTNNIITVESSQYEPKPFYIEGD
                     WSAASYYYSIAILAKEATITLKGLTAQQIQGDAVIADIATKFGVQTTYNNKAVTLTKT
                     AQVQPAHFQYDFLECPDLAQTVVSFCAALQTAVNCNGLQTLRIKETDRIAALDIELQK
                     LGLASLVEIDSNAWKLNILDQSQININLPIHTYEDHRMAMAFAPLVIKTNEILIEEPN
                     VVTKSYPLFWKDLAALGFTISSI"
     gene            complement(5394..5561)
                     /locus_tag="PLL59_03525"
     CDS             complement(5394..5561)
                     /locus_tag="PLL59_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22905.1"
                     /translation="MHAQCAMCKGTVQTSDYAKSINLGIEYMLIVPIVLISLIAYLWM
                     KNKDKFLSKES"
     gene            complement(5669..6223)
                     /locus_tag="PLL59_03530"
     CDS             complement(5669..6223)
                     /locus_tag="PLL59_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HMY22906.1"
                     /translation="MNNNVFQAMITKNDKLAYIVIGFFSLLIFFAITALARVKIDVNL
                     GFDPHIFATINASINFMVSLLLPTGIYFAKQKKYDAHRKVMVIAIVLSSLFLISYVLH
                     HLLTNSTPYPKDAGNIKYFYYFILITHIILAGIILPFVLLTAYRAATAEFPKHKKIAR
                     ITFPIWWYVSITGVIVYCMISPYY"
     gene            complement(6270..7016)
                     /locus_tag="PLL59_03535"
     CDS             complement(6270..7016)
                     /locus_tag="PLL59_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMY22907.1"
                     /translation="MNNSKKTGFIAAVVVVALFPLLFFLFFNKLNNHRPTLETDRCLP
                     IYGPKQAFPSTDYRGRDIIDTAYFKVPDFSFLDQNGDTVTQKLMNGKVTVVDFFFTTC
                     KNICIDMTSNLHKVQETFITDRDVLILSHTVDPESDSVQQLFHYAVEKDVNSKMWRLL
                     TGDKKAIYKQARVGYFITADQGDGGPSDFIHDQKFVVIDKEKRIRGYYDGTDKAAVDQ
                     MIKDIQMLLVSYIVPMKKDDPKYKKTKNRK"
     gene            complement(7019..7345)
                     /locus_tag="PLL59_03540"
     CDS             complement(7019..7345)
                     /locus_tag="PLL59_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HMY22908.1"
                     /translation="MSGHMTDEQYKSAVKAVWSATIILSIVTVVEVVVALAVGHILPK
                     IILNSFFVLASVAKAFFIVGEFMHLKYEKRAFMLTLGVPLAFLVWAIIALAVEGHYWN
                     ILNYPK"
     gene            complement(7380..8129)
                     /locus_tag="PLL59_03545"
     CDS             complement(7380..8129)
                     /locus_tag="PLL59_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMY22909.1"
                     /translation="MAEVVDIHEQHVENQHHLPSPDNGEGGEKSAMNMSYGKTMMWYF
                     LLSDAFTFSAFLISYACIRLAQPWWPNPNSVFKSFPGLGDAYVPLGFVSVMTFILILS
                     SVAVVRAVQEGHRMNKKGVMLWMIGGIVGGVMFLGCQAWEWNHLIHEGLRPFYNPFGP
                     DANGLTHLVHEKMSVSPGPILFGALFFGITGFHGFHVFTGICINIWAFFATANGTFEK
                     RGHYEMIEKVGLYWHFVDLVWVFVFLAFYLM"
     gene            complement(8147..8776)
                     /locus_tag="PLL59_03550"
     CDS             complement(8147..8776)
                     /locus_tag="PLL59_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMY22910.1"
                     /translation="MNLNASLKYKVNNQGAHRFTMYLFIITTSMIFAGFTSAFLVRKG
                     VGGAWSSFGIPSVFIISTVLILLSSVCLHLAHLANKKQNNVMTTIGLFATLVLGALFC
                     YFQIQGWKALNDAGVYLSFNPNPAGPYFLVITFVHAVHVIFGLLFLLIAFFHSLLLLR
                     RKESTHVIDQIESSEKGIFIIRTDLLTLFWHFMGILWVYLYFFLTFNLK"
     gene            complement(8773..9678)
                     /gene="cyoE"
                     /locus_tag="PLL59_03555"
     CDS             complement(8773..9678)
                     /gene="cyoE"
                     /locus_tag="PLL59_03555"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HMY22911.1"
                     /translation="MKAQELHIKDLTLGRLITLKIKDILELTKFRLSFLVVFSAVMAY
                     LIANTGLINWAKVTLLFLGGFFVTASSNAINQLLEKDTDKLMDRTKNRPLPAGRMKPI
                     EASLIAGIFAVAGIFILGVLFNPISGLLGALSLISYAFIYTPFKKISPIAVFIGAIPG
                     SMPLLIGWTAATGSIGIGGIILFAIQFFWQIPHFWAIAWLLNEDYKKAGYYLLPSYEG
                     KTRATALQNIPYLVCLVAVSALPYVLGMTGIISLIISLIAGILFLIQGIQLATDISDK
                     SARKLMFASFIYIPIVFISFVIDKL"
     gene            complement(9699..11519)
                     /locus_tag="PLL59_03560"
     CDS             complement(9699..11519)
                     /locus_tag="PLL59_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMY22912.1"
                     /translation="MQTTTTSEQHVHENAHAHASGHHHTETFVSKYIFSLDHKTIGKQ
                     FLTMGMIWAIIGGFMSVVFRIQLGWPDETFPILERIFSSWFTDGKLNPESYYALITMH
                     GTIMVFFVLTASLSGTFANILIPLQIGARDMASPLMNALSFWLFFFAGVIMFASLFLP
                     TGAASAGWTIYPPLSALPQTSPGSGNGMTFWLIAMVLFVASTLLGGLNYVSTILNMRT
                     KGMTMYRMPLTVWALFFTAIIGILSFPVLVSCVVLLIFDRGLGTSFYLSNIVIGGKLL
                     PYSGGSPILFQHLFWFLGHPEVYIIILPAMGIVSEVLAVHSRKPIFGYKAMIFSILGI
                     TVLSFIVWAHHMFLSGMNPFLGSIFTLFTLLIAIPSAVKVFNWLTTLWRGNIRINPPS
                     MFAIGFVSMFISGGLTGIWLGNSTVDIQLHDTYFVVAHFHIVMGIAAFFGMFAGVYHW
                     FPRMYGRMMNETMAYIHFAITFIGAYLIFWPMHFIGLAGVPRRYYSFNEIEAFAQYGD
                     LNKLISIATIVVFLGQLLFLVNFVYSIYRGKKSTQNPWQANTLEWTSPIEGIHGNWDG
                     ELPTVHRWPYDYSRNPNSDVDFIPQTVPLTEEEKKDKIHH"
     gene            complement(11604..12572)
                     /locus_tag="PLL59_03565"
     CDS             complement(11604..12572)
                     /locus_tag="PLL59_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HMY22913.1"
                     /translation="MSIILGLLTLVLLYIVVVQISKASDLIKSLRSDDEQEESFSNGA
                     LFVAVIGFGVLVYSIVSCFIYGPTILPKPASEQGVWIDNLLKITIVLTGIVFVITQTL
                     LFWFAYKYQYKKDRKAYFFADHNKLEIAWTIIPAAVLTLLIVFGIQKWFKIFSPTPKE
                     AIRIEVVGKQYAWSVRYAGADKKLGKRDFSLVNPDNELGINWNDASSHDDFMVDEIVL
                     PVGKPVSFDIGALDVIHNFWLPHFRLMMSAVPGVPTHLWFRPTVTTEEMKKETNNPNF
                     EYVVACNKLCGSGHYNMKKVVKVVSQEEYEKWFRSQKSYYYSVVKQ"
     gene            complement(12585..13886)
                     /locus_tag="PLL59_03570"
     CDS             complement(12585..13886)
                     /locus_tag="PLL59_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22914.1"
                     /translation="MNTYVFDSSTKKNIFTAFGIGVLLLVLGIFTFNGGQSSNHDAHH
                     ESEKHATAKADNHDAAAEGHGHEAPTVKTALIANSHNALYFFFYIALGAIFFLAATNI
                     AWGGWQIQIQKIPLAMATTIIFFLVGLCIVFALFNHDLFHWTHESLYAKGTATFDEVL
                     NTKRDFLNMKTFWSFTIVISIACALMVIKFWNTFSKLDTNPTRQLFSSSRVISAVSIV
                     VISFLINTFATWLWSMSIQPHWYSTMFTWNTMAGACVTTLSIIILFIHYLKAKGYLPQ
                     VNDNHIHDVAKLMFAISIFWCYTWFAQYMLIWYANIPEETEYFRLRRDFDNYGVLFHG
                     SWVLNFVIPFFILMTRNAKRSVNITIFCAIVIILGQYAAFFLMNCPPLLPKGGFGLIS
                     FGLLLIFGSIFVYLTLMMLAKVKDLTSSTHPYVKESYNHHI"
     gene            complement(13910..14515)
                     /locus_tag="PLL59_03575"
     CDS             complement(13910..14515)
                     /locus_tag="PLL59_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMY22915.1"
                     /translation="MLKKYHFILLILVLGFTACIRNDKKPGREYMPDMSAHIAYEAGS
                     DNPFFKDGKTNQLAPDGSMARGKYIYPLSDTDYEKSASLITNPFQFTSDEINNEGKHL
                     YKVNCAICHGDKGDGQGHLVQINKFPPPPSYLTEPLLSLPDGKRYHTVMYGKGLMGSY
                     ASQLNHKERWLVLSYVKKNLQGISTPAPATTATATDSTKTN"
     gene            complement(14520..15050)
                     /locus_tag="PLL59_03580"
     CDS             complement(14520..15050)
                     /locus_tag="PLL59_03580"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HMY22916.1"
                     /translation="MSDTKKYLVGIFDDDDVLMHAVKDIRKAGYEITDAYTPFPVHGL
                     EHALGLESTRLHTAGFFFAATGFTVALSMITFINMFNYPTDFGGKPYWGLLAYIPITF
                     EHTVLYSAIGMTVSFYYLCKLWPGKTPKIIDERTTDHLFALTFELDGSKDASYINTVS
                     ELLKKEGAIEVYQKEV"
     gene            complement(15120..16559)
                     /gene="nrfD"
                     /locus_tag="PLL59_03585"
     CDS             complement(15120..16559)
                     /gene="nrfD"
                     /locus_tag="PLL59_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HMY22917.1"
                     /translation="MASHFESPLREPLINGNKTYKDITNDIVKPTTGKIQTKWAIYTA
                     ISAVFAGLFVFCIAWEVMFGIGTWGANKTVGWAWDITNFVWWIGIGHAGTLISAILLL
                     FRQKWRTGVNRAAEAMTIFAVVCAGQFPIFHMGRVWDAFFIFPYPNTRGPLWVNFTSP
                     LLWDVFAISTYFTVSLLFWYTGLVPDFGTIRDQSKTSGRWKFYNFLSFGWIGTAKHWE
                     RWESLSLILAGLSTPLVLSVHTIVSFDFATSVVPGWHTTIMPPYFVAGAIFSGFAMVL
                     TLMIVARHIMNLQDYITLAHVESMNKIMLMTGTMVGIAYLTELFVAWYSGNMYEAYAF
                     ANRVSGPYWWAYWSMMFCNLVSPQVFWFRKLRRNIAVTFFMSILINIGMWFERFVIIV
                     ISLHRDYLPSSWLYYSPTWVEVGIFVGTIGLFMFLFLLFCRFFPAIAIAEVKAILKIS
                     GNQYREKQLEEVKDMEVYKETSVKAGTWD"
     gene            complement(16598..19699)
                     /locus_tag="PLL59_03590"
     CDS             complement(16598..19699)
                     /locus_tag="PLL59_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HMY22918.1"
                     /translation="MSNKKYWTSLDELNNTPQYQESAAVEFKDATENDIAEEQSQGGT
                     NRRDFLKVMGFSVSAAALASACKIPTHKAIPYTIDLRKQVPEVVPGVADYFASTFTDG
                     TDFVNILVKTREGRPIKIEPNNDSPLGGTTAHSEASVLSVYDTTRLATPTQKNKKADW
                     KSVDADVTQKLNAITTSGGKIAIVTGTITSVVTKKAIEKFVAKYPSTTVVAYDAVSNY
                     ATRKANQISLGAAIIPTIHFDKAEVIVGFNSDFIGTGISALSNVKKYIANRVPSKENP
                     KMSRHHQFQSNVTITGSNADYKYPIKPSEEKQALINLYAEIGGSISGVKTLSGKDAND
                     GIKKVAKELLAAKGKSVVISDTNDVDVQLLVNAINATLGNYGTVIDTANTSNLKAGND
                     EALAALANDLKAGAYKGIIFLNANPVYNTPYADIFKEAIKKAELSISTADRPDETAQF
                     CTYVCPDNNYLESWDIISPVTGFYGFVQPAISPLFDTRQTAASLLTWAGDTTPIAEVV
                     QAEVATITGANYRAAVQAGFYGSIGVAGGASANAASAIAALSTATASAANGLEVVFYE
                     KSGVGDGSFANNPFLQEMPDPITKVTWDNYVCVPYSFAKEKGIDAQPAEKNVPIAKVT
                     INGKTLELPVVVQFGQAQNTLAIAVGYGREKAGPAGDAVGKNVYTLLKAVNGATSFTT
                     SGATFELTGKEYQLGLNQTYHTLMEDNALPMREKRYRGSIVKETTLSDYAKNEKSGNE
                     DREHIKEHFSHSLYDGYKPNDYKQGHHWGMVVDLNACIGCGACVVACSIENNVPVVGR
                     RQVYRSQEMHWLRIDRYYSGNPDNPDVAFQPMMCQHCNNAPCENVCPVNATNHSSDGL
                     NQMAYNRCIGTRYCANNCPYKVRRFNWLDYQGVDSFGTFNDGKMSFTDTQAQSYMFED
                     LTRMVLNPDVVTRSRGVIEKCSFCIQRIQEGKLTAKKEGRPVKDGDIKSACQTACSTN
                     AIIFGDQNDKESVVAKTFETNGRNYFVLEEQHFLPAVGYQVKVRNKDEQPAEFFIDKP
                     EKA"
     gene            complement(19760..21049)
                     /locus_tag="PLL59_03595"
     CDS             complement(19760..21049)
                     /locus_tag="PLL59_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMY22919.1"
                     /translation="MHSCKPIRKILALLVFLLLLTQSTSTFAAFAVPDVAATDAAGVE
                     KVKSGMTLFKANCTACHALDRKVVGPALAGVWEKWESEEKFVHWVQNPAKLIASGDAY
                     ANKIFNENNKVMMTAFPTLSADQIKDILLYLKNPAAAEPAKAPAADATASSSSGAATG
                     DKGSSNWYVWILAAFLAVLALLLWSVTSKLDKAVKEKAGEKVEDESLTKKLFSNKTLK
                     VLLLIGSILLVFNIFNGAINLGRSQGYAPDQPIKFSHALHVGQNQIECQYCHTGVEKS
                     RHASIPGTQICMNCHKYVQQGPKFGKDEIAKIYDYSGWDPDKGKFVKAPKNIEWVKIH
                     NLPDHVYFNHSQHVKVGGIDCKTCHGKVEEYEVMKQFAPLSMGWCINCHRQTEVKFAD
                     NNYYSIFQKYKAELKKGTKAKVTVADIGGTECQKCHY"
     gene            21369..22517
                     /locus_tag="PLL59_03600"
     CDS             21369..22517
                     /locus_tag="PLL59_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22920.1"
                     /translation="MTIVIFNSCQSIKKSFESKDYDKVITLFLKENNFDDNELSMFEQ
                     SYNAAFERDKQQVLLLKSINNGERWEEIFNLYTKINNRQNEVLRLLPLHYTNGEEAKI
                     ETFNFGNVLEESKLNAAQYYYDQGLKLYNSGKKSAIRESVDYFTASTKFFINYKDVNE
                     LIEQAKIRGKNHVLILIDKNPALLVPPAFEQQILNNIKITKNNTWVEIENYKNSNKEY
                     DYVVKLNIYDIQVSPDILKEYNYTEEKTIADGWQYVLDDRGNVKKDSLGNDIKVQKYK
                     KISCQVRETRMSKTAQVFGDATIYNASTRQIQKQDRCTGNTAFNYSYYQYNGNRDALS
                     SSTLSKLNIPPTMFPSSLDMVGQSASEITRCYSDFINKNYSLFSYTAP"
     gene            22527..23120
                     /gene="rsmG"
                     /locus_tag="PLL59_03605"
     CDS             22527..23120
                     /gene="rsmG"
                     /locus_tag="PLL59_03605"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HMY22921.1"
                     /translation="MQIIKKYFPNLTPTQLHQLEQLLPLYREWNEKINVISRKDVANF
                     YERHVLHSLSIAKVIQFEKGEKIVDIGTGGGFPGIPLAILFPESFFILSDSISKKILV
                     VNEVVSALSLQNVEAITCRAESIQQDIDYVVTRAVAPMKDIIFWSKKMHAKKIIALKG
                     GDLKEELEGIKKNIRIYDLKDQFEEDFFETKKIVVCS"
     gene            complement(23182..24618)
                     /locus_tag="PLL59_03610"
     CDS             complement(23182..24618)
                     /locus_tag="PLL59_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMY22922.1"
                     /translation="MHNRIKIFGLLSIIILLFTSCNIPKVVQRPENTTTPLTYNNKNS
                     SDTINTATLSWRTFFADSNLVHLIDTALVYNQELMKTLQEIEIAKNDVRRKKGALLPS
                     VNYGFDVDADKPGRYTSQGAGDEATNITENKKIPVVLPNVKLGLDMEWEIDIWKKLRN
                     SKKAAIQRFLASVEGKNFVLTNLIAEIANSYYELLALDNQLVVIHQNITIQKNALEVV
                     KIQKDAARTTELAVQKFEAEVYGTQSKEFEILQQIRETENRINLLIGRFPTTIPRSSE
                     NFETMVPMFVSEGIPAQLLQNRPDLKQAALELSATKLDVKVAKAEFYPTLKISASLGL
                     EAFNPAFWIKLPASIFANLAAGLLGPLFNRNAITAEYLNANAEQKKAVYNYEQHILSA
                     YLEVSTQLYAIDNLQKNYSYKSLQIGALNKSIDIANDLFKNARADYFEVLMTQRDALE
                     TKLELIETKKEQLNAVVNLYKSLGGGWH"
     gene            complement(24611..27775)
                     /locus_tag="PLL59_03615"
     CDS             complement(24611..27775)
                     /locus_tag="PLL59_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMY22923.1"
                     /translation="MFSKFIQRPVLSIVISLIIVFLGVIAITQLPVTQFPSISPPKVN
                     ILTEYPGANGELMIKSVIIPLERALNGVPGMKYMTSDAGNDGEANIQIVFNLGTDPNV
                     AALNVQNRVASVTNKLPPLVIREGVKVSREESNMLLYVNLFSKDTKADQRFLFNYADI
                     NLLPELKRVNGVGYADILGNREYAMRIWLKPDRMLAYKISTEEVMEALSSQSLEASPG
                     KTGESSGKRSQAFEYVIKYTGRFTTEEQYKNVILRANADGELLRLKDIADIEFGSSMY
                     DIYSNLNGHPSAAIVLKQSYGSNASQVIKDVKAKLKELQKSFPKGMEYEISYDVSTFL
                     DASIEKVVHTLIEAFILVGIVVFLFLGDWRSTLIPALAVPVSLIGTFAFMQLFNITLN
                     LITLFALVLAIGVVVDDAIVVIEAVHANMETKHLSARKATAEALKEISGAIIAITFLM
                     AAVFIPVAFMSGPVGLFYRQFSITMATAIILSGIVALTLTPALCAMILKNNHANKKKA
                     TLVTKSLFYFNNAFDKSASKFVQFLSKVVNRRRITALVLLLFCVFTFLINKTVPAGFI
                     PNEDQGMFYAIIQTPPGSSLERTNEIAERLQKIAEHIDGIKSVSALAGYEILTEGTGA
                     NSGTCLINLKDWSERKHNVVEIIQELEEKTKDISGATIEFFQPPAVPGYGAAGGFELR
                     LLDKTGNNNYKEMENVNNDFVKALSKRPELTSVFSFYSASFPQYMLKVDNDIAQQKGV
                     TIENAMSTLSTLMGSNYEISFIKFGRMYKVIVQAAAQYRALPEDILKLYVKNNKDEMV
                     PFSDFVSMEKIYGLSETTRHNMYNSSEISGAAAPGYSSGEAIDVIKEVAEKNLPRGYG
                     IDWAGISADEVAQGNQALYIFLICLGFVYLILAGQYESFILPLSVILCLPAGIFGAFL
                     FLKIFGLENNIYAQVAMVMLIGLLGKNAVLIVEFAALRHSQGLSIYKATLEGVKVRFR
                     PILMTSFAFIAGLIPLMFATGPGRIGNRTIGTAAAGGMFIGTLFGLFLIPGLYFIFGT
                     IAAKYQHIKDEEENPLTEEIDNHA"
     gene            complement(27793..28872)
                     /locus_tag="PLL59_03620"
     CDS             complement(27793..28872)
                     /locus_tag="PLL59_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMY22924.1"
                     /translation="MTRSFYYLLFVIPFLFTNCNSTTVEKEEVIKYVVTTPIQTDTSS
                     SKEYVAQIRSFRNIEIRTQEKGFLQNVYIDEGQSVKAGQVLFRIMPKIYEAEMMKEQA
                     EVNAAEIEVSNAKALVDKNVISKNELLLAQAKLGQAKAEATLARVHLSFTEIRAPFDG
                     IIDRLPKKIGSFLDEGEFLTSLSDNSKMFAYFNVSEPEYLNYKMQAKNNNTQVELKLA
                     NNEILPAKGKIETIEAEFNNETGNIAFRATFPNADRLLKHGQTGKIIMTVPLKNAILI
                     PQKATYEIQDKKYVFIVDKNNIVHSKNITISGELPDIYVVSEGLKSSDKILLEGIQKV
                     KDDEKIQYTFDDANNVLKHLKLKAE"
     gene            complement(28959..>29311)
                     /locus_tag="PLL59_03625"
     CDS             complement(28959..>29311)
                     /locus_tag="PLL59_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22925.1"
                     /translation="MKSKVFILFTLLLFVFQKGNAENHAINKSTYKLTEISNPSIGIP
                     TNVLPTIGSFLFENNQIAEEVDAPQVKFLHSFSLSTFYIHSILTLFLFLLFIVASINI
                     AKDKIYIFFRVFRI"
BASE COUNT         9671 a         5248 c         4730 g         9562 t
ORIGIN      
        1 cttccacatt caggtttgct ttatgcaacg ctctcgggat tgcttttgcc ggtgtggtgg
       61 taaaccattc cggatcctga gaagcatctg caaaagatac aatctttgcc aatggttgca
      121 cacccagttc ctttaccttt tctccactca tcagcaccat ggcactggca atagccactc
      181 ttttggtggc caatatttgg aattgcaaga aaattctttt ttacaataca cagatgtttt
      241 tgaagaccca aatatgcctg atgtaatggt tacttctgtt tacttaaaac aagttgcctg
      301 tggcactgaa ttaaaaatta cacaagaaaa tatacctgca gcaattcctg tagaaatgtg
      361 ctacttaggc tggcaagaat cgcttgagaa attaaaaaaa ttagtagaac caaacattcc
      421 tgatggaata tgatggcaat aaattaattc tataaattaa tttacatact atagcaaaca
      481 gacacgacct atccttaatc atctatcaac aaataataat aaaaaatgct tggcatctac
      541 caagcatttt ttattataaa aagtgtcaaa catattgaga ttaaaaacaa taagtatttg
      601 cttctaacac ttttgctgca acacgtctgc gtgcttcttt tgtattcaca atgtcgtatt
      661 ttgtataacg ttttaagccc attaacataa tacgcaattc atcaccttcg ccaaaagctt
      721 gtaaggcatg tttgccatat aaattgattc tttccatacc atcagaaatt gcaattttcg
      781 tcatatcaat ttgtacagca acagcagctt cgccattatt tgcaattagt ttttgtgtgc
      841 gtaatacagt tgactccaag gcatacactt ccgccaacat gtccgcaata ttcattaata
      901 tttcttgttc atttttgagt tgtttcatca acttttgaac ggcggcacca gctgtcatta
      961 aaatagcttt tttagcattt tgtactgcct ttaattccgc agccaatggc tcgttatctt
     1021 cattggcact aaaatctggc acactcatta attctttttg cacagctaat gccggcgtca
     1081 tcatatctat tttgccactc atagcgcgct ttaacaacat atctacagag agcaaacgat
     1141 tgatttcatt ggtaccttca aagattctat tgatacgtgc atctctgtat gcgccagctg
     1201 ccgctaattc ttcagagtaa cccattccac catgaatttg tacattttca tcgacacaat
     1261 aatctaaaac ttcagaacct atgaatttta ataatgaaca ttcaatagca tattcttcag
     1321 ctgccatcat gatggcttct tggtaaggtt tgccttgtgc tactaattct ttttctttat
     1381 tgtcaatcat atcagaagca cgataacacg cactttgtaa tgcataaata cgtgtagtca
     1441 tttccgctaa tttgtattga atagcaccga aattagaaat cggtgtttta aattgttggc
     1501 gctcttctgc atattttaaa gattgctcta aagcagattt tgaaccacct aacacgcctg
     1561 ctgctaattt ataacgacca atatttaaaa tattaaatgc aatcaaatga cctttaccaa
     1621 tttcacctaa taaattttct ttcggtactt ttacattttc caaaaacacc tgacgtgttg
     1681 aagaaccttt aatgcccatt ttcttttctt ctgctcctaa tgtcaagcct ggcatatctt
     1741 tttctaaaat taagcatgaa aatttatcgc caccaatttg acagaaaacg ataaaaacat
     1801 cagcaaatcc agagtttgta atccacattt tctgaccatt aacaatgtaa tgtgtgcctt
     1861 cttcatttaa aattgctact gtttttgcac ctaatgcatc agagccagaa ctaggttccg
     1921 ttaagcaata acaagctttt agctcgccac tcgctaactt cggcaagtag tgtgtttttt
     1981 gttgttcgtt gccaaaatag acaatcggca aagtgccaat gccaatatgt gctccaagtg
     2041 ataatgaaaa agaacgagat ttagctaaat tttcgaccaa caatgaattg gtattaaaat
     2101 ctttgcccat gccgccatat tcttcaggaa ttgccaaacc caacaatcct aattcaccag
     2161 cttttgctaa taatgattct gttaaaccag gctcttggtg ttcaattctg tcgtagattg
     2221 gtgttatttc agtttccaca aatgacttca ccgtatcaac tatcatcagt tgttcttcat
     2281 tcaaatcttc tggtgtaaat acgtcttgga aatttgatga tttaactaaa aactcaccac
     2341 cattcagcaa gttttttact tcagttaatg tttcgctcat tgtacttttt ttagtttatt
     2401 tttaaattca ttttcctacc atttatagca gaaagtaata cgctataaga tgatactttt
     2461 tatttttatg taattaaaaa tacaatgcat gcattgaatt accaaacgtt tggtcaaaat
     2521 gattcgttaa aaatttgtta tcaaattagt catgatggaa atcataacaa gaattgcaat
     2581 tttggtttat ttttgtaaaa aactacacta ttcgaactat ggcatcgtac aaacgcattc
     2641 ctttaaaacc atttccaaac ggctggtatg tgctagcaca ttcacatgaa gtaaaaaatg
     2701 gtgaaattat cacacgaaga tttgctggga aagatattct gttatttcga acagcaagcg
     2761 gcaaactaag tgttagcgat ccttactgtc cgcacatggg tggtcattta ggctatggtg
     2821 gcgttgttga aggcgacagc ataaagtgtc cattccatca tttttgtttc gatacaaaag
     2881 gcgattgtac ggctactggt tatggttcta aaccatcttc aaaattaaaa ttacctgtat
     2941 atcattttga tgagaaaaat gatttgattt taatttggtt tgatgaaaaa aatgcggcac
     3001 caacttggca tgttccacaa gaagattgga gcgagtggac agcagtgcgt tttgcagaat
     3061 atgatttcaa ttcgcatcca caagaaacct cagaaaacag tgtagatgtt ggacatttcg
     3121 gcatcgtaca tggttacact ggcgtagaaa cattgcatga aatggaaagt catgaacatt
     3181 acttatatgc caaatatgca atgaatcgcg tcgctgattt tgttggaaaa aaaagaacnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca acaattttat tatgatgatt
     3361 cgacacaagt caactatatt ccgcaagaaa tagtataaaa tactccatac ttttttatta
     3421 attgtacatc aatatttttg atgaaagaaa acaattctca aacaattatt atttttgttt
     3481 gttatcaaaa aaagctatca aaataggcat gttatttgta taaattagtc aaattgaaaa
     3541 aatgaaaaac tacgtatttt tattgttatc cttaacctta ttgacaacat caactttcgc
     3601 gcaagaaaat tggaaattag agaaagacaa aaatggtatt aaagtatgga atagaaaggt
     3661 tgctggcaca gcaattaaag aatttaaagt atcaactatt atcaatacat caacagaaaa
     3721 aatgttggca tttttaaaaa acacagctaa gtatgaccaa tggatgtata aggtagatga
     3781 aggcagcgta aaagtgttga aacgtgtcag tgacaacgat tactatactt acatgacgat
     3841 ttctgcgcca tttataaaaa caagagaaac tataacacac atgactttta attcgcaaga
     3901 tgcgaaaggt gctatcttaa ttaatttaga tgctgcaccc aatttattac cattgaatga
     3961 taagtatgtt cgtattccga aaatgaaagc atatttcaaa attgttccac ttggcaatgg
     4021 tcaaattgag cttatacatc aggcgttggg ttcgtctggt ggcaacattc ctgaggcttt
     4081 aataaatcta acttctgtag attgtccttt ttatatgttt accaaaatta aatcgatgat
     4141 atagtaaaac ctagtgctgc cagatctttc caaaataaag gatatgattt tgtaacaaca
     4201 ttcggttctt cgattaatat ttcatttgtt tttatgacta gtggtgcaaa tgccattgcc
     4261 attctatgat cttcgtaagt atgaataggt aagtttatat ttatttgcga ctgatctaaa
     4321 atattaagct tccaagcgtt gctatcaatt tcaacaagtg aagctaaacc taatttttgc
     4381 aactcaatat ccaaggctgc aattctatct gtttctttga tacgtagtgt ttgcaatccg
     4441 ttacaattca cagcagtttg caatgcggca caaaagctca ctacggtctg tgctaaatcg
     4501 ggacattcca aaaaatcata ttggaaatgt gctggctgta cttgtgctgt tttagtcaat
     4561 gtaacagcct tattattata agtagtttgc acaccaaatt tagttgcaat atctgcaatt
     4621 acggcatcac cttgtatttg ttgagccgtc aaacctttta aagttattgt agcttctttt
     4681 gctaaaatgg caatggaata gtagtaagaa gcagcactcc aatcgccttc aatataaaaa
     4741 ggttttggct cgtattggct actttctact gtaattatat tattagtaaa tgtcgatttt
     4801 acaccaaaat attccattat tttgagtgtc atttttatat aaggtacaga aactatttta
     4861 ccaattaact ccaatgttaa accttcttgt aaagtaggtg caattaataa tagtgcagtg
     4921 atatattgtg aagaagtatc agcaggcaaa ctaatttttc ctccttttaa ttttttacct
     4981 cttattcgaa gtggtggaaa tccttccttg tttacataag aaatatctgc acctaattgc
     5041 aataaagcat ctactaaaat ttttattggg cgctgttgca tgcgttcgct acctgtcaac
     5101 acgacttctc taccttcttg cgtggacaaa taagccgtta aaaagcggaa tgtagtgcca
     5161 ccggcaccag catctaaaac agcatcatta gattgtaata tttgttgtaa gaaaactgta
     5221 tcatctgcgt tagaaatatt ttctagttgc aattgatgat tgcataaggc atcaattatc
     5281 aaaacgcgat tgctgatact cttagagcca tttaattgta cttctccttg aatacaatca
     5341 gaagaaggtt ttgttatttt ataaattaat gacatagtgg aaagtgcgat attttagctt
     5401 tccttagaca ggaatttatc tttattcttc atccataaat acgcgataag actgattaaa
     5461 acaatcggca caatcaacat atattcgata cccaagttga tacttttagc atagtcagag
     5521 gtttgcacgg tgcctttaca cattgcacac tgtgcatgca taaagaaact attagcactc
     5581 aatagcaaga caagaattaa gctagtatat tttttaatga acatattcaa aagtacaaaa
     5641 aaaggaattt ataaaaatga atatagcgtt aataatatgg cgaaatcata caatatacaa
     5701 tcacgccagt gatgctcaca taccaccata ttggaaaagt aattcgtgca atctttttat
     5761 gttttggaaa ttcagcagtt gcagcacgat aggctgttag caaaacaaat ggcaatataa
     5821 tgcctgccaa gatgatatgt gtaattaaga taaaatagta gaaatactta atgttgccag
     5881 cgtctttcgg atatggcgta gaatttgtca ataaatgatg taatacataa cttatcagaa
     5941 acaatgaaga taatacgatg gcaattacca ttaccttacg atgagcatca tacttttttt
     6001 gtttcgcaaa atagatacca gtaggcaaca ataaggagac cataaaatta atggaagcat
     6061 ttattgttgc aaaaatatgt ggatcaaacc ctaagtttac atctattttt acgcgtgcca
     6121 atgctgtgat agcgaaaaaa atcagtaagg aaaaaaaacc aataacgata tatgctaatt
     6181 tatcgttttt tgtaatcatt gcttgaaata cattattatt cataagctta attaaataaa
     6241 aataaattgg aattacaaaa gataaaacac tatttcctgt tttttgtttt cttgtattta
     6301 gggtcgtctt ttttcattgg aacgatgtag ctgaccaata gcatttgaat atcttttatc
     6361 atttggtcaa cggctgcttt gtctgtgcca tcgtaatagc cacgaattct tttttcttta
     6421 tcgatgacca caaatttctg atcgtgtata aaatccgaag gaccgccatc accttggtcg
     6481 gctgtgataa aatagccaac acgtgcttgt ttataaatag cttttttatc gccagttaat
     6541 aaacgccaca ttttagaatt tacatctttt tcaacagcat aatggaataa ttgctgtaca
     6601 gagtcacttt ctgggtcaac tgtatgcgat aaaattaaaa catctctatc tgtaataaaa
     6661 gtttcttgta ctttatgcaa attcgatgtc atatcgatac aaatattttt acaagtagta
     6721 aaaaagaaat ctactacagt aacttttccg ttcattaatt tctgtgtaac ggtatcgcca
     6781 ttttggtcaa ggaaagaaaa atcaggaact ttaaaataag ctgtatctat gatatctcta
     6841 cctctataat ctgtagatgg aaaagcttgt tttggcccat aaataggtaa acatctatcc
     6901 gtttctaagg tcggtctgtg attattgagt ttattaaaaa acaagaaaaa caataatgga
     6961 aacagcgcta caacaacgac agcggcaata aatccagttt tcttgctatt attcactact
     7021 atttaggata attcaatata ttccagtagt gtccttcaac agctaaagca atgattgccc
     7081 aaactaagaa agctaaagga acacctaagg ttagcataaa tgcacgcttt tcatatttta
     7141 agtgcataaa ctcacctacg atgaaaaatg ctttcgctac acttgctaaa acgaagaatg
     7201 agtttaaaat aattttgggt aaaatgtgac ctacagctaa ggctactacc acttctacaa
     7261 cagtaacaat acttaaaatg attgttgcag accaaactgc ttttactgcc gatttatatt
     7321 gttcatctgt catgtgaccg ctcatataat tagattttga gagttttaaa taattaatgt
     7381 tacattaagt agaatgctaa gaatacgaat acccaaacta aatctacaaa gtgccagtat
     7441 aaaccaactt tttcaatcat ttcgtagtga ccgcgttttt caaaagtacc atttgccgta
     7501 gcaaaaaatg cccaaatgtt tatacaaatt cctgtgaata cgtgaaaacc gtgaaagcca
     7561 gtaataccaa agaatagcgc accaaacaag attggaccag gagaaacaga cattttctcg
     7621 tgtactaagt gtgtcaaacc attagcatct ggaccaaaag gattataaaa cggacgtaag
     7681 ccttcatgta ttaaatgatt ccattcccaa gcttggcagc ctaagaacat cacaccacca
     7741 acgataccac caatcatcca aagcataacg ccttttttat tcattctatg gccttcttga
     7801 accgcacgaa caacagctac tgagcttaga attaaaataa atgtcattac actcacaaag
     7861 cctaatggaa cgtatgcatc tcctaaacca gggaatgatt tgaatacgga gttaggattt
     7921 ggccaccatg gttgcgccaa acggatacaa gcgtaagaga ttaaaaatgc agaaaatgta
     7981 aacgcatctg aaagcaagaa ataccacatc atggttttgc cataactcat attcattgct
     8041 gatttttcac caccttcgcc attatcaggg cttggtaaat gatgctgatt ttctacatgc
     8101 tgctcgtgta tgtctactac ttctgccatt attgaaattt tattgtctat tttaaattaa
     8161 aagttaagaa gaaatacaaa tatacccaaa gtattcccat gaaatgccaa aacaaagtca
     8221 gcaaatcagt acgtatgata aatattcctt tttctgatga ttctatttga tcgattacat
     8281 gcgtgctttc ttttcttctt aaaagtaata aagaatgaaa aaatgcgatt aacaaaaata
     8341 acaatccaaa aataacgtgt acagcatgta cgaatgtaat tactaagaaa tagggtccag
     8401 ccggattggg attaaaagac aagtaaacgc ctgcatcatt taatgctttc cagccttgaa
     8461 tttggaaata gcaaaacaaa gctcctaaaa ctaatgttgc aaataatcct attgttgtca
     8521 ttacattgtt ttgtttttta ttggcaagat gtgcaagatg caagcataca ctgcttaata
     8581 aaatcaaaac tgtagaaatg ataaatacag atggaatacc aaaagacgac caagcaccac
     8641 caacaccttt gcgcactaaa aacgcactcg tgaaacctgc gaaaatcatt gatgtggtga
     8701 tgatgaatag gtacatcgtg aagcgatgtg ctccttgatt gtttacttta tattttaaag
     8761 atgcatttaa attcataact tatctattac aaatgaaata aatactattg gaatatagat
     8821 aaaagaggca aacatcaatt tacgtgcact tttatcagaa atatctgttg ctaattgtat
     8881 gccttgtatt agaaataaaa taccggcaat taaactaata attaatgaga tgataccagt
     8941 catacctaat acatacggca aagcactcac cgcaactaag cacactaaat aaggaatatt
     9001 ttgtaaagca gttgcacgtg tttttccttc ataagatggc aacaagtaat agcctgcttt
     9061 tttataatct tcatttagca accatgcaat agcccaaaaa tgaggaattt gccaaaaaaa
     9121 ttgaatcgca aataagataa ttccaccaat tccgatagaa ccagttgctg ctgtccaacc
     9181 aattaataaa ggcatggaac ctggaattgc accaataaaa actgcaatag gacttatttt
     9241 tttaaatggc gtataaataa aagcatacga aataagcgac agtgcaccta ataaaccact
     9301 gataggatta aatagaacac ctaaaataaa aatgccagct acagcaaaaa taccagcaat
     9361 taaagatgct tctattggct tcattcttcc agcaggcaat ggacgatttt ttgttctgtc
     9421 catcaatttg tctgtgtctt tctctaataa ttgattaatt gcattagaag aagctgttac
     9481 aaaaaaacca cctaaaaaga gtaatgttac ctttgcccaa ttaatcaaac ctgtattagc
     9541 aattaaatat gccattacag cagaaaatac cactaagaac gataatcgga attttgtcaa
     9601 ttccaatata tccttaattt tcaaggtaat taatctacca agagtcaaat ctttaatatg
     9661 taattcttgc gctttcaaaa tattaaaatc aactacaatt aatgatgaat tttatctttc
     9721 ttttcttctt ctgttaaagg aacagtttga ggaatgaaat ctacatcgct atttggattg
     9781 cggctataat cgtatggcca acgatgaact gtaggcaatt cgccatccca gtttccgtgg
     9841 ataccttcaa ttggagatgt ccattctaaa gtatttgctt gccaaggatt ttgagtagat
     9901 tttttacctc tataaatact gtaaacaaag tttaccaaga ataacaattg acctaagaaa
     9961 acaacgatgg tagcaatact gattaattta ttcaaatctc cgtactgagc aaatgcttca
    10021 atttcgttga aagaataata acggcgagga acacctgcta aaccgatgaa atgcattggc
    10081 caaaaaatca agtaagcacc aataaatgta atagcaaagt gaatgtaagc cattgtctcg
    10141 ttcatcattc taccatacat acgtgggaac cagtgataca caccagcgaa cataccaaag
    10201 aatgctgcaa tacccattac aatatggaaa tgtgcaacaa cgaaataagt atcgtgtaat
    10261 tgaatatcta cagtagagtt acctaaccaa atacctgtta aaccaccaga gataaacatg
    10321 gatacgaatc caatagcaaa catagatggt ggattaattc tgatattacc acgccataaa
    10381 gtggttaacc agttgaatac ttttacggct gatggaatcg caattaataa agtaaataat
    10441 gtaaaaatag agcctaagaa tggattcata ccagacaaga acatgtggtg tgcccaaacg
    10501 ataaagctta atactgtaat acctagaatt gagaaaatca tcgctttgta accgaagatt
    10561 ggcttacgag aatggacagc taatacttca gaaacgatac ccattgctgg caagatgatg
    10621 atatatactt cagggtgacc taagaaccaa aataagtgtt ggaataaaat aggtgaacca
    10681 cctgaatatg gcaataattt accgccaata acgatattag ataagtagaa actagtacct
    10741 aatcctctat caaaaatcaa caaaactaca catgatacta atacagggaa cgataagata
    10801 ccgataatag cagtaaagaa caatgcccat actgttaaag gcattctata cattgtcatg
    10861 cctttagtac gcatatttaa aatggtagaa acatagttaa gaccacctaa taatgtagaa
    10921 gcaacgaata acaccattgc aattaaccaa aaagtcatac cattaccaga accaggtgat
    10981 gtttgaggca aagcactcaa aggaggatag attgtccagc cagcagatgc agcacctgta
    11041 ggtaagaata aggaagcaaa cataatcacg cctgcaaaga aaaataacca gaacgatagc
    11101 gcattcatta atggagaagc catatctcta gcgccaattt gtaatggaat aaggatattg
    11161 gcaaaagtac cgcttaagct cgctgttaat acgaagaaca ccataattgt accatgcatt
    11221 gtaattaatg cataataaga ttctggattt aattttccat ccgtgaacca agaagagaat
    11281 attctttcta agattgggaa tgtttcgtct ggccagccta attggatacg gaaaaccact
    11341 gacataaatc caccgatgat tgcccaaatc atacccatcg ttaaaaattg cttaccaatt
    11401 gttttatggt ctaagctgaa aatgtattta gaaacaaacg tctccgtatg gtgatgaccg
    11461 gaagcatgtg catgtgcgtt ttcatgtaca tgttgctcag atgtggtagt tgtttgcata
    11521 tttttagttt atactaatga attatacttg ttacttattt ttcaaaatat ctagctgata
    11581 gaaatcaaca gaaatggaat agattattgt ttaactacgg aataataata tgatttttga
    11641 cttctgaacc atttttcata ttcttcttga gaaactactt ttaccacttt tttcatgtta
    11701 tagtgaccag aaccacataa tttgttacaa gcaacaacat attcaaagtt tggattattc
    11761 gtttcttttt tcatttcttc tgttgtcact gttggtctaa accataaatg cgtaggcaca
    11821 ccaggaactg ctgacatcat taagcggaag tgaggcaacc agaaattatg aattacgtct
    11881 aatgcaccga tgtcaaaaga aacaggtttg ccaactggta aaacgatttc atcgaccata
    11941 aaatcatcat ggctagatgc atcgttccaa ttaattccta attcattatc aggatttact
    12001 aaagaaaaat ctcgtttacc taatttttta tctgcacctg cgtaacgaac agaccaagca
    12061 tattgtttac caacaacttc aatccgaatc gcttcttttg gtgttggtga aaatattttg
    12121 aaccattttt ggataccaaa tacaattaat aaagtcaata cagcagctgg aataattgtc
    12181 caagcaatct ctaatttatt gtgatcagca aagaaatatg cttttctatc ttttttatat
    12241 tgatatttat aagcaaacca gaataataat gtttgtgtga taacgaaaac aatacctgtt
    12301 aaaacaatag taattttcaa taaattatca atccaaacac cttgttctga cgcaggtttt
    12361 ggtaaaatag taggaccata aataaagcag ctaacaatgg aatatactaa aactccaaag
    12421 ccgattactg ctacaaataa tgcgccattg ctaaaagatt cttcttgttc atcatccgat
    12481 cgtaatgact ttattaaatc ggacgctttg ctgatttgta caactacgat gtacaacaac
    12541 actaacgtta ataggcctaa tataattgac atttgtgact atttttaaat atggtgatta
    12601 taactttctt ttacatacgg atgtgtgctt gaagttaagt ctttaacttt tgccaacatc
    12661 atcagtgtta aataaacaaa tatacttcca aaaatcaata acaaaccaaa gctaattaaa
    12721 ccgaagccac cttttggtaa taatggagga cagttcatta agaagaatgc tgcatattga
    12781 ccaagaataa taacgattgc acaaaagatg gtgatattta cacttctttt tgcatttcta
    12841 gtcattaaaa taaagaacgg aataacaaag tttaaaaccc aagaaccatg gaatagtaca
    12901 ccataattat cgaaatctct gcgtaaacgg aaatattcag tttcttctgg aatattagca
    12961 taccaaatta gcatgtattg tgcaaaccaa gtatagcacc agaatatact aatagcaaac
    13021 atcaatttag ctacgtcgtg gatatggtta tcatttactt gaggtaaata gccttttgct
    13081 tttaggtaat gtataaataa aataattatg gaaagtgtgg taacacaagc accagccatc
    13141 gtattccacg taaacatagt actataccaa tgaggttgga ttgacatcga ccacaaccat
    13201 gttgcaaaag tatttattaa gaaagaaatg acaactatag atacagcaga aattacacgg
    13261 ctactagaaa ataattgtct cgtagggtta gtatctaatt ttgaaaatgt attccaaaac
    13321 ttaattacca taagtgcaca tgcaatagaa ataacaatgg tgaaagacca aaatgttttc
    13381 atattcaaga agtctctttt ggtgtttaat acttcatcaa atgttgcagt tcccttagca
    13441 tataaggatt catgtgtcca atggaaaagg tcatggttaa atagcgcaaa tacaatacat
    13501 aaacctacta agaagaatat gattgttgta gccattgcca atggaatttt ttgtatctgt
    13561 atttgccagc cgccccaagc aatattagtt gcagccaaga aaaatattgc acctaatgcg
    13621 atataaaaaa agaaatataa agcattatga gaatttgcaa ttaatgctgt ttttactgtt
    13681 ggcgcttcgt gtccatgacc ttccgctgca gcgtcgtgat tatcagcttt tgctgttgca
    13741 tgtttttccg actcatgatg tgcatcatgg ttactagatt gtccaccatt gaaagtgaaa
    13801 atacccaaca caagaagcaa cactcctatt ccaaaggcag taaaaatatt tttcttggtg
    13861 ctcgaatcaa aaacgtatgt attcattgcg agtgtctatt taatttggtt taattagttt
    13921 tagttgaatc cgttgctgtt gctgtagttg caggagcagg cgttgaaata ccttgtaaat
    13981 tctttttcac atagctcaat actaaccaac gttctttatg gtttaattga gaagcatacg
    14041 agcccatcaa tccttttcca tacattacag tatgatatct ttttccatct ggtaaagaca
    14101 ataatggttc cgtaaggtaa gaaggtggcg gtggaaattt attaatttga actaaatgac
    14161 cttgaccatc acctttatcg ccatggcaga ttgcacagtt tactttgtac aaatgttttc
    14221 cttcgttatt gatttcatcg cttgtaaact ggaaaggatt tgtaattaat gaagcagatt
    14281 tctcataatc tgtatcagac aaaggataga tatattttcc tcttgccata cttccatctg
    14341 gagccaattg atttgttttt ccatctttaa agaaaggatt atcagaacca gcttcgtaag
    14401 caatatgagc tgacatatct ggcatatatt ctctaccagg ttttttgtcg tttctaatac
    14461 aagctgtaaa gccaagtacc agaattaata gtatgaaatg atattttttc aacatgatat
    14521 tatacttctt tttgataaac ttcgattgcg ccttcttttt tcaataattc agaaactgtg
    14581 ttgatatagc ttgcatcctt tgatccatct aattcaaatg ttaaagcaaa taagtggtca
    14641 gtagtacgct catcgataat ttttggcgtt tttcctggcc atagcttaca taagtaatag
    14701 aaagaaaccg tcataccaat ggcagagtaa agcactgtat gttcaaatgt aattggaata
    14761 taagctaata aaccccaata tggtttacca ccgaaatctg ttggataatt gaacatattg
    14821 ataaatgtta tcatacttaa tgcaacagta aaacctgtag cagcaaaaaa gaaacctgcg
    14881 gtatgcaacc ttgttgattc taaacctaag gcatgttcta atccgtgaac aggaaacggc
    14941 gtataagcgt cagttatttc ataaccagct ttacgtatat ctttaacggc gtgcattagc
    15001 acatcatcat cgtcaaaaat tcctactaaa tatttttttg tatcgctcat attttttagt
    15061 attaagtagt aagtaaaaag tattaaggct ttatttactt tatactataa atttatttat
    15121 taatcccaag ttccagcttt tacagaagtt tctttatata cttccatgtc ttttacttct
    15181 tctaattgtt tttcgcggta ttgatttcca gatattttta aaatcgcttt cacctctgca
    15241 attgcaatag cagggaagaa tctacagaac aataagaata agaacatgaa taaaccgatt
    15301 gttcctacga agatacctac ctctacccaa gttggagaat agtataacca gcttgatgga
    15361 agataatctc tatgcaaaga aataacaatg attacgaaac gttcgaacca catacctata
    15421 ttaattaaga tagacatgaa gaaagtaaca gcaatatttc ttcttaattt tctgaaccaa
    15481 aatacctgtg gagatactaa gttacagaac atcattgacc aatatgccca ccaataagga
    15541 ccagaaacac ggttagcaaa agcataagct tcatacatat ttcctgaata ccaagccaca
    15601 aataattctg ttaagtaagc aatacctacc atggtaccag tcattaacat gattttattc
    15661 atggactcta cgtgcgccaa tgtaatgtag tcctgtaggt tcataatatg acgtgctacg
    15721 atcattaatg ttagcaccat tgcgaatcca gagaaaatag caccagcaac aaagtaaggt
    15781 ggcatgattg tcgtatgcca accaggtaca actgatgttg cgaagtcgaa agatacaatt
    15841 gtatgtactg ataatactaa aggtgtagat aaacctgcta aaattaagga tagtgattcc
    15901 cagcgttccc aatgttttgc agtaccaatc caaccgaagc ttaagaagtt ataaaacttc
    15961 catctgccag atgtttttga ttggtcacga atggtgccaa aatctggcac taaacctgtg
    16021 taccaaaaca acaatgatac tgtgaaatag gtagatatcg caaacacgtc ccataataat
    16081 ggagaggtaa agtttaccca tagtggacct cttgtatttg gataagggaa gataaaaaat
    16141 gcgtcccaaa cacgacccat gtggaaaata ggaaactgac ctgcgcagac aaccgcaaag
    16201 atggtcattg cttctgctgc acggttaaca cctgtacgcc atttttgacg gaataataat
    16261 aagatagcag agataagcgt acctgcgtga ccgataccaa tccaccaaac gaagttggtg
    16321 atatcccatg cccaacctac tgttttattg gcaccccaag taccgatacc aaacattact
    16381 tcccatgcga tacagaaaac aaatagacca gcaaatacag cagaaatagc tgtataaatt
    16441 gcccatttag tttgtatttt acctgtcgta ggttttacga tatcattcgt aatatcctta
    16501 taggtcttat tgccgttaat taacggttcg cgtagtggtg attcaaaatg acttgccatt
    16561 atatcttgct ttactacaaa ttttatttac aattatgtta tgctttttcc ggtttatcga
    16621 taaagaattc tgccggttgt tcgtctttat ttctaacttt tacttgatag cctactgctg
    16681 gtaaaaagtg ttgttcttct aatacgaagt aatttcttcc attagtttca aatgtttttg
    16741 ctactacact ttctttatca ttttggtcac cgaagatgat tgcatttgtt gaacatgctg
    16801 tttgacatgc agatttgata tcgccatctt taactggacg accttctttc ttagctgtca
    16861 atttaccttc ttggatacgt tggatacaga atgagcattt ttcgataaca ccacgtgagc
    16921 gcgtaacaac atctggattt aataccatac gtgttaaatc ttcaaacata taagattgag
    16981 cttgtgtatc tgtgaatgac attttaccat cattgaatgt accgaaacta tctacacctt
    17041 ggtaatctaa ccagttaaaa cgacgtactt tatatggaca gttgtttgca caatatctag
    17101 taccgataca tctgttatag gccatttggt tcaaaccatc agaactatgg tttgttgcat
    17161 ttactggaca aacattctca caaggagcat tgttacagtg ttggcacatc atcggttgga
    17221 aggccacatc tggattatct ggatttccac tataatatct atcgatacgt aaccagtgca
    17281 tttcttgaga gcggtaaact tgtcttctac caacaacagg aacgttgttt tcgatgctac
    17341 aagcgaccac acaagcacca caaccgatac atgcattcaa atcaaccacc ataccccagt
    17401 gatgaccttg tttgtagtca tttggtttat agccgtcgta taatgagtga ctgaaatgtt
    17461 ctttgatgtg ctctctgtct tcgttaccag atttttcatt tttagcataa tctgataatg
    17521 ttgtttcttt aacgatagag cctctatatc ttttttcacg cattggaagt gcattatctt
    17581 ccattaatgt gtgataggtt tgattcaaac ctaattgata ttctttacct gttagttcaa
    17641 atgttgcacc acttgtagta aatgatgtag cgccattcac tgcttttagc aatgtataga
    17701 catttttacc aacagcatcg cctgcaggac cagctttttc gcggccataa ccaacagcga
    17761 ttgctaatgt attttgagct tgaccaaatt gaacaacaac aggtaactct aatgttttac
    17821 cattaattgt aacttttgca attggtacat ttttttcagc tggttgtgca tcgatacctt
    17881 tttcttttgc aaatgaataa ggaacgcaaa catagttatc ccaagttact ttagtaattg
    17941 gatctggcat ttcttgtaag aatggattat ttgcaaatga accatcgcca acacctgatt
    18001 tctcatagaa tactacttct aaaccgtttg cagcacttgc tgttgcagta cttaaagctg
    18061 caattgcact cgctgcgttt gcacttgcgc caccagctac gccaatacta ccatagaaac
    18121 ctgcttgaac agcagcacga tagttagcac ctgtaatagt agccacttcc gcttgtacta
    18181 cttcagcgat tggagtagta tcaccagccc aagttagcaa agatgcagct gtttggcgag
    18241 tatcgaataa tggagaaata gcaggctgaa caaaaccata gaatccagta actggagata
    18301 tgatgtccca agattctaaa tagttattat ctgggcaaac ataagtacaa aactgtgctg
    18361 tttcatctgg tctgtcagcg gtagatattg ataattcagc ttttttgata gcctctttaa
    18421 aaatatctgc gtatggtgta ttataaacag gattagcatt taagaagatg atacctttgt
    18481 atgcacctgc ttttaagtcg ttagcaagcg ctgctaaagc ttcatcatta cctgctttta
    18541 agttagatgt atttgctgta tctattactg taccgtaatt tcctaatgtt gcattgatag
    18601 catttactag caattgtaca tcaacatcat ttgtatcaga gataacaaca gattttcctt
    18661 ttgctgccaa taattctttc gccacttttt tgataccatc atttgcatct tttccgctta
    18721 gtgttttaac accagaaata gatccaccaa tttcagcata caagttaata agtgcttgtt
    18781 tttcttctga aggtttaatt ggatatttat aatctgcgtt agagccagtt attgtcacat
    18841 tagattggaa ctggtggtga cgcgacattt taggattttc cttacttggt actctgttag
    18901 cgatgtattt ttttacatta gaaagtgcag atatgccagt accaataaaa tctgagttaa
    18961 aaccaacaat tacttctgct ttatcgaagt gaattgtagg aataattgca gcgccaagac
    19021 ttatttgatt tgctttacga gtagcataat tagatacagc atcataagcc accactgtag
    19081 tgcttggata tttcgctaca aatttctcga tagctttttt cgtaactaca gaagtaatag
    19141 tacctgtaac gatagcaatc ttgccaccac tagttgtgat agcatttaat ttttgtgtta
    19201 catcagcatc gactgatttc cagtccgctt ttttattttt ttgtgttggc gtagccaatc
    19261 ttgttgtatc gtatacactc aacacagatg cttcagaatg tgctgttgta ccacctaatg
    19321 gcgagtcgtt attcggctca attttaatag gtcttccttc acgtgttttt actaaaatat
    19381 ttacaaagtc agtgccatct gtaaatgtag aagcaaaata atcagcaaca cctggaacta
    19441 cttctggcac ttgctttctc aaatcgattg tataaggaat tgctttgtgt gtaggaattt
    19501 tacaagcaga tgccaatgca gctgccgaca cactaaaacc cattactttt aagaagtcgc
    19561 gacgattagt accgccttgg ctttgttctt cagcaatatc gttttctgtt gcatctttaa
    19621 attcaactgc tgctgactct tggtattgag gagtattgtt taactcatct aaactcgtcc
    19681 aatatttttt gttactcatt tatatataat gttataacgt ttatatttaa tttatatgaa
    19741 ggcgtatttc gtctttattt taatagtgac atttttgaca ttccgtacca ccgatatctg
    19801 ctactgttac ttttgctttt gtaccttttt tcaattctgc tttatatttt tggaatatag
    19861 agtagtaatt attatctgca aatttcactt ccgtttgtct gtggcaattg atacaccagc
    19921 ccattgatag aggagcaaat tgtttcatta cttcgtactc ttctacttta ccgtggcaag
    19981 ttttacaatc gattccgcca acttttacgt gttgagagtg gttgaagtaa acatggtctg
    20041 gtaagttatg aattttcacc cattcgatat tttttggtgc ttttacgaat ttacctttat
    20101 caggatccca gccagaatag tcataaattt ttgctatttc atctttacca aattttggtc
    20161 cttgctgaac atatttgtgg cagttcatac aaatttgcgt accaggaata cttgcatgtc
    20221 ggcttttttc aacacctgta tgacaatatt gacactcgat ttgattttga cctacgtgta
    20281 atgcatgtga gaatttgatt ggttgatctg gagcatatcc ttgagagcga cctaaattta
    20341 ttgcgccatt aaagatattg aacactaata aaattgaacc aatcaacaat aatactttaa
    20401 gtgttttatt gctaaataat tttttagtta atgactcgtc ttcaactttc tcgccagctt
    20461 tttctttaac cgctttatct aattttgatg taacactcca taataataat gctaaaacag
    20521 ctaagaaagc tgccaaaatc caaacatacc aattagaaga acctttgtca ccagtagcag
    20581 caccacttga acttgaagct gtagcatcag cagcaggtgc ttttgctggc tctgctgcag
    20641 ctggattttt taagtataat aaaatgtctt tgatttgatc agcactcaat gtcgggaatg
    20701 cagtcatcat tactttatta ttctcgttaa agattttatt cgcatacgca tcgccgcttg
    20761 caataagttt tgccggattt tgtacccagt gaacaaattt ttcttcactt tcccattttt
    20821 cccaaacacc tgcaagtgct ggaccgacaa cttttctgtc tagtgcgtga caagctgtac
    20881 aattcgcttt aaatagcgtc atacctgact tcactttttc aacacctgca gcatcagtag
    20941 cagccacgtc tggcacagca aatgctgcga atgtagatgt actttgagta agaagcagta
    21001 ggaatactaa tagagcgagg atttttctga ttggtttaca agaatgcatt atttatctaa
    21061 tttttctcgg ttgcaaatat agtattcagc tactaaaact cctaaagtgt tgataattta
    21121 ctatttagaa tcaatataaa taatatacac tttattgatt atcaatattt atagaatgac
    21181 ttgtgattat aaaaaaacag aatttaatgt actcttaaca tttatttagg ttttgtgtta
    21241 atattcggga actttattat tcaaatttca gttttagcgt ctatttttta taatttggta
    21301 cgatttttaa cagtctattt taagaataaa aaatgatgaa aaacatttgc agctccactt
    21361 tgctttttat gactattgtg atatttaatt cctgtcagag tataaagaaa agttttgaaa
    21421 gtaaagatta tgataaagtg attacacttt tcttaaaaga aaataatttt gatgataatg
    21481 agctgtccat gtttgagcaa tcctataatg ctgcattcga acgcgacaag cagcaagtac
    21541 ttttattaaa atctatcaat aatggtgagc gatgggaaga aattttcaat ttatacacaa
    21601 aaataaataa tagacaaaat gaagtattgc gtctattacc attacattac acaaatggag
    21661 aagaggcaaa aatagagact tttaattttg gaaatgtatt agaagaatca aaacttaatg
    21721 ctgcacaata ttattacgat caaggattga aactttataa cagtggaaaa aaaagtgcga
    21781 tacgtgagtc tgttgattat ttcactgcct ctacaaaatt ttttatcaat tataaagatg
    21841 taaacgaatt aattgagcaa gcaaaaatta gaggaaaaaa tcatgtattg atacttatag
    21901 ataaaaatcc agctttatta gtgccaccag cgtttgaaca acaaatatta aacaatataa
    21961 aaattacaaa aaacaatact tgggtagaaa tagaaaatta taaaaacagc aataaagaat
    22021 atgattatgt agtgaagtta aatatctacg acatacaagt atcacctgat attctcaaag
    22081 aatacaatta taccgaagag aaaacaattg ctgatggttg gcaatacgta ttagacgaca
    22141 gaggcaatgt aaaaaaagat tctttaggta atgacataaa agtgcagaaa tataaaaaaa
    22201 tatcatgcca agtgcgtgaa acacgtatga gcaaaacggc acaagtattt ggtgatgcta
    22261 ctatatataa tgcatcgact agacaaatac aaaagcaaga cagatgcaca ggaaatactg
    22321 cttttaatta ttcctactat caatacaatg gcaatagaga tgcattaagt tcgagtactt
    22381 taagcaaatt aaatattccg ccaacaatgt ttccttcatc tcttgatatg gtaggtcaat
    22441 ctgcatcaga aataacaaga tgctattctg attttataaa caagaactat tctctatttt
    22501 cctacactgc accataaatt aatagtatgc aaatcatcaa aaaatatttt cctaatttaa
    22561 ctccaacaca acttcatcaa ttagagcaat tattgccatt atatcgtgaa tggaatgaaa
    22621 aaataaatgt aatatctagg aaagatgtag ctaattttta tgaacgacat gttttgcatt
    22681 ccttatccat tgccaaagtt atacaatttg aaaaaggtga aaaaattgta gatattggca
    22741 caggtggcgg atttccaggc attccactag ccattctttt tccagaatca ttttttattt
    22801 tatcagattc tatttcaaaa aaaattctag ttgtcaatga agttgtcagc gcattatctt
    22861 tacaaaatgt agaagccatt acctgcagag cggaaagcat acaacaagat attgactatg
    22921 tggtaacacg tgctgtggcg ccaatgaaag atataatttt ttggagcaaa aaaatgcatg
    22981 ctaaaaaaat tattgcactg aaaggcggcg atctgaaaga agaattagaa ggcatcaaaa
    23041 aaaacatacg catctatgat ttaaaagatc aatttgaaga agattttttt gaaaccaaga
    23101 aaatagtagt ctgtagttaa tagaaaaatt agtcctttta tataaaaaat attacgttgt
    23161 agatatcaaa caaatcgtaa actaatgcca gccgccacct aaacttttat aaagatttac
    23221 tacggcattt aactgttctt tcttagtttc gataagttct aattttgttt ccaaagcatc
    23281 gcgttgggtc atgagcactt caaaataatc tgctcttgca tttttaaata aatcattagc
    23341 tatatcaatt gatttgttta aagcacctat ctgcaaagat ttatatgaat agtttttttg
    23401 caaattatca attgcataaa gctgtgtaga aacttctaag taggccgaca agatatgttg
    23461 ttcataatta tacacagctt ttttctgttc tgcgtttgca tttaaatatt cagctgtgat
    23521 ggcatttcta ttaaaaagtg gtcccaacaa accagctgct aaattggcga atatagaagc
    23581 aggtaattta atccaaaatg ctggattaaa tgcttctaaa cctaaacttg ccgaaatttt
    23641 tagtgttgga taaaattcag ctttcgccac ttttacatct aatttagttg ccgacaattc
    23701 caaggctgct tgttttaagt caggtctatt ttgcaagagt tgtgcaggaa taccttcact
    23761 aacaaacata ggcaccattg tttcaaaatt ttcagaagat cttggtattg ttgtcggaaa
    23821 acgacctatg agtaagttga tgcgattctc tgtttcgcga atttgttgta gtatttcaaa
    23881 ttctttactc tgtgtgccat atacttctgc ttcaaatttt tgaacagcca actcggttgt
    23941 tcttgctgca tccttttgta ttttcactac ttctaaagca tttttttgaa tcgtgatatt
    24001 ttgatgtatc actactaatt gattatctaa tgcaagtaat tcataataag aatttgcgat
    24061 ttcagcaatt aaattagtca atacaaaatt cttaccttca acggaagcca gaaagcgttg
    24121 tatggctgct ttttttgaat ttctcaactt cttccaaata tcaatttccc attccatatc
    24181 caatcccaat ttgacgttag gcaatactac tggtatcttt ttattttctg tgatatttgt
    24241 agcttcatca ccagcacctt ggctcgtata tcgtccaggt ttatctgcat caacatcaaa
    24301 accatagttt acacttggca gtaatgctcc tttttttcta cgcacatcat tttttgcaat
    24361 ttctatttct tgcaaagttt tcatcaactc ttggttgtac actaatgcag tatcgattag
    24421 atgcactaaa ttagaatctg caaaaaaggt acgccaagac aatgttgctg tattgatagt
    24481 atctgacgaa tttttattat tgtaggtcaa tggcgtagtc gtgttctcag gacgttgtac
    24541 tacttttgga atattgcaac ttgtaaaaag taaaataatt atacttaata aaccgaaaat
    24601 ttttattcta ttatgcatga ttgtcaattt cttctgttaa tggattttct tcttcgtctt
    24661 ttatatgttg atattttgcg gcaatcgttc caaaaataaa gtacaaacca ggaattaaaa
    24721 acaaaccaaa gagagtaccg atgaacatac cacctgctgc tgcagtacca attgttcgat
    24781 taccaattct gccaggacca gtagcaaaca ttaatggaat taaaccagct ataaatgcaa
    24841 atgatgtcat taagatgggt ctgaaacgta ctttcacacc ttctaatgtt gctttataaa
    24901 tagataaacc ttgactatgt cttagtgcgg caaactcaac aattaagaca gcatttttac
    24961 ctaataaacc aatcaacatg accatggcaa cttgtgcgta aatattgttt tctaagccaa
    25021 atattttcag aaacaagaac gctccaaaaa tgccggcagg caaacacaag atgacagaaa
    25081 gtggtaaaat aaaactttca tattgaccag ctaaaatgag ataaacaaag cccaaacaaa
    25141 tcaagaaaat atatagtgcc tgattacctt gtgctacttc atctgcggaa ataccagccc
    25201 aatcgattcc ataaccacga ggcagatttt tttctgctac ttcttttatc acatcaatgg
    25261 cttcgccgct gctataacca ggagctgcag caccgcttat ttctgaagag ttgtacatat
    25321 tatgtcgtgt agtttcagac aagccatata ttttttccat ggaaacaaaa tcagaaaaag
    25381 gcaccatttc gtctttatta tttttgacgt acaatttcaa aatatcttca ggcaatgcac
    25441 gatattgtgc agcagcttgt acaataactt tatacattct accaaatttt ataaaactga
    25501 tttcgtagtt gctgcccatt aatgttgaaa gcgtgctcat ggcattttct atagttacac
    25561 ctttttgttg tgcaatatca ttatccactt ttagcatata ttgaggaaaa cttgcactat
    25621 aaaagctaaa cacagatgtc aactcagggc gtttggaaag tgctttgacg aaatcattat
    25681 ttacattttc catttcttta taattattgt ttcctgtttt atctagcaat cgcaattcaa
    25741 agccacctgc agctccataa ccaggaacag ctggtggttg aaaaaattcg atagtagcac
    25801 cactgatgtc ttttgttttt tcttcaagtt cttgaataat ttctacgaca ttatgcttgc
    25861 gctcactcca atcttttaaa ttgattaaac aagttccaga atttgcacct gttccttcgg
    25921 ttaatatttc atatcctgca agcgctgaaa cagatttgat gccatcaata tgttccgcaa
    25981 ttttctgtaa acgttcagca atttcattag ttcgttctaa tgaagaacct ggtggtgttt
    26041 gaatgattgc ataaaacata ccttgatctt cattaggaat aaaacctgct ggcacagttt
    26101 tatttattaa aaaagtaaac acacaaaaca ataatagaac cagtgcagta attctacgtc
    26161 tattgactac tttacttaag aattgtacaa attttgatgc tgatttatcg aaagcgttat
    26221 tgaaataaaa taagcttttt gtgaccaaag ttgctttctt tttattagca tgattatttt
    26281 tcaaaatcat agcgcaaagt gctggcgtta gcgttagtgc cactatacca gaaagtataa
    26341 ttgcagtagc catggtgatg gaaaattgcc tataaaacaa gccgactgga ccagacataa
    26401 atgcgacagg aataaatact gctgccatca aaaaagtgat agctataatg gcgccagaaa
    26461 tttcttttaa tgcttcagca gttgcctttc gggcggaaag atgcttggtt tccatatttg
    26521 catgtactgc ttcaattaca acaattgcat catcgactac tacaccaata gctaatacta
    26581 aagcgaataa ggtaatgaga ttaagtgtga tattaaataa ttgcataaat gcaaatgttc
    26641 cgattaatga tacaggaact gccaatgctg gtattaaagt ggaacgccaa tcaccaagaa
    26701 aaagaaatac aacgatgcct actaatataa atgcttcaat caaagtgtgt actactttct
    26761 ctatggaagc atctaagaat gtagatacat catagctaat ttcatattcc atgccttttg
    26821 gaaaactttt ttgcagttct ttcaattttg cttttacatc tttaataact tgactagcat
    26881 tgcttccata cgattgtttg agtacgattg cagcagatgg gtgtccattc aaattcgaat
    26941 agatgtcgta catagagctg ccaaactcaa tgtcagcaat atctttaagt cgcaataatt
    27001 caccatcggc atttgctctc agaattacat ttttatattg ttcttccgtc gtaaatctgc
    27061 cagtatattt tatgacatat tcaaaagctt gtgagcgttt tccagaactt tctcctgttt
    27121 ttcctggtga tgcttctaag ctctgactcg acaatgcttc catcacttct tctgttgaga
    27181 ttttataagc taacatacgg tcaggtttta accaaatacg catggcatat tctctgttgc
    27241 ccaatatatc agcatatcca acgccgttta cacgttttaa ttcaggcaat aaattaatat
    27301 cggcgtagtt gaataaaaat cgttgatctg ctttagtgtc tttactaaat aaattgacat
    27361 ataaaagcat attcgattcc tcacgtgata cttttacgcc ttctcgaata actaatggtg
    27421 gtaatttatt agttacagat gcaacgcgat tctgaacatt taatgctgcc acgtttggat
    27481 ctgtgcctaa attgaaaact atttgaatat tggcttctcc atcatttcct gcatcagaag
    27541 tcatatactt cataccaggc acaccattca atgcgcgctc taatggtatg ataactgatt
    27601 taatcatcaa ttcaccatta gcacctggat attctgtaag aatatttacc ttaggtggtg
    27661 agatagatgg gaattgtgtg acaggtaatt gtgtaatagc gatgacacct aaaaacacaa
    27721 taataagtga tattacaata gacagtactg gtctttgtat gaatttacta aacatgattt
    27781 ctgattgtta aattattctg cttttaattt taaatgtttg agcacattgt ttgcatcatc
    27841 aaatgtgtat tgaatttttt catcatcttt gactttctga attccttcta acaatatttt
    27901 atcagatgat ttcaatcctt ctgacacgac ataaatatca ggcagttcgc cacttatcgt
    27961 tatatttttc gaatgcacga tattgttttt atctacaata aaaacatact ttttatcttg
    28021 aatttcgtaa gttgcttttt gtggtattag aatagcattt ttcaaaggca ccgtcataat
    28081 tatttttcca gtttgaccat gctttagcaa tctgtctgca ttaggaaatg tagcacgaaa
    28141 agcgatattg ccagtttcat tattgaattc agcttctatt gtttctatct taccttttgc
    28201 tggaagtatt tcattgttag ctaattttaa ttcaacttgt gtgttattgt ttttcgcttg
    28261 cattttataa tttaaatatt caggttctga cacattgaaa taagcaaaca ttttactgtt
    28321 gtcggataaa cttgttagga attcaccttc atctaaaaag cttccaattt tcttcggcaa
    28381 tcgatcaata atgccatcaa aaggtgcacg aatttctgtg aatgataaat gcactcgagc
    28441 taatgtagct tctgctttag cttgtcctaa ttttgcttgt gctaataaca attcattttt
    28501 tgaaatcaca tttttgtcca ctaatgcttt tgcattggat acttcaattt cagcagcatt
    28561 aacttctgcc tgttctttca tcatttctgc ttcataaatt ttaggcataa tacgaaataa
    28621 gacttgtccg gcttttacac tttgtccttc atcgatataa acattttgca aaaagccttt
    28681 ttcttgcgtt ctaatttcaa tatttctaaa agaacgaatt tgtgcgacat actctttgct
    28741 agacgatgta tctgtttgaa ttggtgttgt aactacgtat ttaattactt cttctttttc
    28801 tactgttgta gaattacaat ttgtaaataa aaaaggaatg acaaataaaa gataatagaa
    28861 tgaccttgtc ataatagata aataattttt tagatgatgt atgaaaatag agccaatcaa
    28921 acagaagttt gattgtaaag tgtgagttgg aaatggtgtt atattctaaa taccctgaag
    28981 aaaatataga tcttatcttt agcaatattt attgacgcta caataaatag caaaaacaaa
    29041 aatagtgtga gaattgaatg aatataaaat gtagataaag agaatgaatg caaaaacttt
    29101 acctgtggag catctacttc ttctgcaatc tgattatttt caaatagaaa tgaacctatt
    29161 gtcggtaaca catttgttgg tattccaata gacggattac tgatttctgt aagcttataa
    29221 gtcgacttat tgatggcatg attttcagca ttgccttttt gaaaaacaaa taataataag
    29281 gtgaaaagta taaagacttt tgatttcatt t
//
LOCUS       DAOQQV010000019        29241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_468_length_29241_cov_8.930686, whole genome shotgun sequence.
ACCESSION   DAOQQV010000019 DAOQQV010000000
VERSION     DAOQQV010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 29241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29241
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_468_length_29241_cov_8.930686"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLL59_03630"
     CDS             complement(<1..193)
                     /locus_tag="PLL59_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22926.1"
                     /translation="MLHQINFKKYGLWSMLCALFLFTACRYSKEPKTFENKDISIQFP
                     SYLFEADDVYPTKNSILQAK"
     gene            complement(211..942)
                     /locus_tag="PLL59_03635"
     CDS             complement(211..942)
                     /locus_tag="PLL59_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HMY22927.1"
                     /translation="MSINIYNSAALPVPVKIIVEARRSSRVSLGKNEVIVRLPKHISV
                     ADKEKIVKQFLMWAKQQIQDNNYYQFHQNKIEVYHNKQLHIFDTPFTIELEYIASGRN
                     KLSYRGDHILKIYLLQNIPEVEQKHHIQRFLLRFTEKYFLSKIQERTIYWNERYFKEK
                     IEKIDIKHTVSRWGSCSSSRKLSFATKLLLLPCEIIDYVIVHELAHLKEMNHSPKFWK
                     HVENAMPAYQTHRKWLQQHGSKIDF"
     gene            complement(1000..1665)
                     /locus_tag="PLL59_03640"
     CDS             complement(1000..1665)
                     /locus_tag="PLL59_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HMY22928.1"
                     /translation="MNNLSAVEENYLKYIYQLGETQNGIVKTNDLAYKLEHSAASVTD
                     MLKRLSDKKLVSYKKYYGVSLTKIGQQIAIQVMRKHRLWETFLAKNLKFTWDKIHDIA
                     EQLEHIQSDELVDKMDEYLGYPKFDPHGDPIPDKQGNILVPNVICLTDAKLKKLYILQ
                     NVNDDSASFLKYLNKIQLQLNDKIKIIEKEEFDETMQIIVNDKRQITISNDAAQNMFV
                     KSV"
     gene            1739..3067
                     /locus_tag="PLL59_03645"
     CDS             1739..3067
                     /locus_tag="PLL59_03645"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HMY22929.1"
                     /translation="MWNNHSNWKFERTTNSLPEVFSSIDVPKNVQGWRKLLAFTGPGL
                     MVAVGYMDPGNWATDIAGGSSFGYTLLSVILISNLFAILLQHLCVKLGVVTGRDLAQA
                     CKDSYSKPVSFLLWIICEIAIAACDLAEVIGSAIAINLLFGIPLTVGIIITILDVLVL
                     LMLQNKGFRYLEAFVGSLIFIIFCCFAYELFMAKPAIAAFMNGLLPTTEILTNADMLY
                     IAIGILGATVMPHNLYLHSSIVQTRNFERNDEGKKMAIKYATIDSTASLLMAFFINAA
                     ILILAASAFHYNGHKEVADIHDAYKLLEPVLGVKMASTLFAIALLASGQNSTLTGTLA
                     GQIVMEGFLNIRLKPWLRRLITRLIAVVPALLVSILYGENGTGKLLVLSQVILSVQLS
                     FAVIPLLQITSNKTKMAQFANSAFLHYTAWIIALIIIIFNAVMLWNMFFA"
     gene            complement(3079..3543)
                     /locus_tag="PLL59_03650"
     CDS             complement(3079..3543)
                     /locus_tag="PLL59_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22930.1"
                     /translation="MSNKIILFPFWLNTILYVVSLAITGFYCSQWKIFSSEKKEDKKV
                     SSIFNGVLAIVLAFVLLSAFRLPINFVIQQQAKKNEIITAVCPITFYSIGLKNQTKKH
                     ISFSFQNKSIRMKVRPSTMAKIKSLSTPNKHITLQLRKSILGTYVVENYDVY"
     gene            complement(3615..4148)
                     /locus_tag="PLL59_03655"
     CDS             complement(3615..4148)
                     /locus_tag="PLL59_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HMY22931.1"
                     /translation="MLDYNYIKALHIIFVVNWFAGLFYMPRLFIYTAEANDKMEPEKS
                     ILIAQFKIMQRKLWYIITWPAAIITLIFGVLLLIQSGFLSQTWMHIKLAFVLGLYVYH
                     FSLHRIFSQQQKNIFKYTSTQLRIWNEVATIFLFAIVFLVVVKTNLSFLTGLIGLFIL
                     VILLMLGIKLYKLLRKE"
     gene            complement(4290..5624)
                     /locus_tag="PLL59_03660"
     CDS             complement(4290..5624)
                     /locus_tag="PLL59_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22932.1"
                     /translation="MKKIFLVVNVLLLTWSVANAENCARYIDGDSTKTTPAYSIYREF
                     FKKGLYADALPQWRNMFLKAPGFRKQTHLDGITIYSALIQKENDKTLRQAYIDTLFQI
                     YNKQIECFGDDEYVLGKKAMDLQKYGKNTDLPEVLSLLDKYIKITGEKAFYYYVQTYF
                     KVLVNQVGKNGITEEYAINKYDEITTIVDKNIANTSSKYNKEYKEVKSVIDDLYTTNF
                     ADKSNPEDCAKLLEIYLKKYKANPNDLATIKSVYLKTKGCADSTTNVELLEKLNKLAP
                     NYTFATRLANIYIKYKMYDTAIVLYQNALAVESDTLKKADLNYYISFMKYAKEDFPAS
                     RDFAKEAIKYNPLHSRAYNLIGLLYLSSGPLCGPGTGFQSQIVLWPAFDYFNKVIEIN
                     ADADLVTEAQKYISDYSKYLPTKAEITAKKLTVGGPYTVKCWINEETTVKVK"
     gene            complement(5641..7038)
                     /locus_tag="PLL59_03665"
     CDS             complement(5641..7038)
                     /locus_tag="PLL59_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22933.1"
                     /translation="MRKIFLLFILAITLQQVDAQEHTPYSRYGLGHAFDNNTAQSAQM
                     GGLGAAFQSGETVNYLNPASYAALQLTTFDGGFSGNFGKIKTQTLKAKQNSFSLNYLS
                     LFFPVKKFFVTGVSLLPFSAKDYLISQTTSFDTANAVKFEYEGTGSLYNLSWGAGYKY
                     KGFHVGFNLGYLFGKLNNNSFAYPINKVGTFDAESYTTWTFTNTKVSSIIWNAGVQYN
                     LPITSKKDSNKIYNVVFGLSGNAPTKVSKRTYNDEALYTFQSGYLAYRTSDAGINDFI
                     TDYIQPKVSSTDMLDTLSEKFNERAYIKIPATLNIGIMAYRGIKWRAGIDFKFQPWSK
                     YRGYEDNAASKLNNSWRIAVGGEFLPNDKNFSKFFSRLKYRAGFNYTQTNIKINNQAI
                     NEFGINFGIGIPLIITTANDQGLLQKVYTYAFNIGFEAGSRGTTQKNLVRENFYRLKF
                     GISLNDKWFVRRKYY"
     gene            complement(7031..7750)
                     /locus_tag="PLL59_03670"
     CDS             complement(7031..7750)
                     /locus_tag="PLL59_03670"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HMY22934.1"
                     /translation="MNIAIDIGNTRTKIGIFDKQEMRSLITYDNSKKIAFEEIFATHN
                     ISKSIISSTVEIPNYIIQKLASLPQHLILNETTRLPFHNCYSSKETLGKDRLALVAAA
                     TAMFPNQNTLVIGCGTCITFNFINAQNEFLGGSIHPGLKMRLKAMHTFTGKLPLAALI
                     SDADLMANNTEANLQSGVLYGASKEIDGMISDYLVKFSEMNTIITGGDSDLLVYRLKN
                     QIFAISNFTLIGLNHILEYNA"
     gene            complement(7779..8981)
                     /locus_tag="PLL59_03675"
     CDS             complement(7779..8981)
                     /locus_tag="PLL59_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMY22935.1"
                     /translation="MSFRKISAHKIYPISSSPIENGVIIIDENGKIIALDVMDKHDAT
                     TVEFFNGVIVPGFINAHCHLELSHLQGVAQTGTGLIDFISQVIKNRDHSQEIIQDAIA
                     REENNMLQSGIVAIGDISNTTDTFSQKRKAHLHYYTFVEYFDLFQDQNTANTIEQYDA
                     VFNALLETDKNKKSKVPHAPYSVSKKLFQHIIARNNEDDAYLPKTISIHNQETMAENE
                     LFLHKKGGFIDFYERIGFTTEAIPNIGQSAIHYALNFLNKADRNLFVHNTLSTKEDIA
                     RAQQQLGADHTFWATCPNANLYIENSLPNYNNFIVNNSQVCIGTDSLTSNWQLNILEE
                     MKTILKYQSYLSFETVLTWATLNGAKALGFEDVLGSIQVGKTPGLVLIENMENNILMA
                     NSVSRKIV"
     gene            9047..10087
                     /gene="rlmN"
                     /locus_tag="PLL59_03680"
     CDS             9047..10087
                     /gene="rlmN"
                     /locus_tag="PLL59_03680"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HMY22936.1"
                     /translation="MQTQIDIRTLSKEELIAQLAPLGEKPFRAKQIYEWLWKKSAVDF
                     DEMTNLSKDLREKLKQYFLLLPIRAYKVQKSNDGTIKSAFQLHDKHLIEGVLIPTEDR
                     ATACVSSQVGCSLSCSFCATGFLKRERNLSAAEIYDQVVLINQQAIESIGRNLSNIVF
                     MGMGEPLLNYNNVMSAIDKITAEEGLNMSAKRITVSTAGIAKMIKKLADDNARFNLAL
                     SLHAADDEKRNTIMPINEQNNIDALIDALQYFQSKCKGEVTFEYILLDGVNDSIDDAK
                     KLVAICKRLIDVKINVIEYNKIEQADFHKSTVETREKFIDYLNKHKVIANVRKSRGKD
                     IDAACGQLANKS"
     gene            10217..10696
                     /locus_tag="PLL59_03685"
     CDS             10217..10696
                     /locus_tag="PLL59_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMY22937.1"
                     /translation="MQQAFLQTINQHQGIIHKVCRIYCNNQDDHNDLFQEITLQLWKA
                     FPSFRSEAKISTWMYRIALNTAISGIRKKKIQTTALENINFQPVDFNDTSTEDNMLLL
                     YKAIAYLSDVEKSIVLLYLEDKPYDEIAEITGITANYVAVKMNRIKEKLRTLLKTVQ"
     gene            10693..11331
                     /locus_tag="PLL59_03690"
     CDS             10693..11331
                     /locus_tag="PLL59_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22938.1"
                     /translation="MIELDDLKNIWSSNIEHDIGKQTLEKENIKALLRHKSVSIIDKL
                     RRNLLLEIAMFFVCLLLIAGVPFYFKSKEIAIICVVVILFTFVPYLFYYIKKYKELQR
                     FFSYHENIKSNLELLIVQLEKYLNIYFWGSLLLTPISGFLSGYAILYELKALGFLLYF
                     DMFNSGTLTIILSFALLLTLLSYPIMKWYIRKFYGQYLEKLKDCLKELQEIE"
     gene            complement(11333..12271)
                     /locus_tag="PLL59_03695"
     CDS             complement(11333..12271)
                     /locus_tag="PLL59_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HMY22939.1"
                     /translation="MNISLTQLEYILAIDNYRHFAKAAEACLVTQPTLSMQLKKLEEE
                     LNIDIFDRSKQPIIPTDAGKLLIEQARIVLREAKKLEQIAKHYDDSLEGDFTIGIIPT
                     IAPYLLPYFISDFLKKYSKIRLIIQELTTEDIVFKLNKDELDAAILATPLHEINLIEE
                     PLFYEEIKVYAHYNHPLTKKKNLSHEDIQRDDIWMLSNGNCFRNHVINICTNKMRVND
                     RLKYESGSIETLKKMVEMEGGFTLLPELAVLELPTKKLPQLAEFKKPKPLREVSIVYA
                     RSVAKKKFIDLLAKLIIDNIPDVLLDKKRGTVVEWK"
     gene            12356..12841
                     /locus_tag="PLL59_03700"
     CDS             12356..12841
                     /locus_tag="PLL59_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HMY22940.1"
                     /translation="MKKTKHITQIGLDTKQSETLASQLNVLLANYQLYYQNARGLHWN
                     IKGKHFFQLHTKFEEIYTDAQEKIDLIAERILTLGFTPLHTFSDYLKHTTIKEGKQIS
                     DANEAVALVISGLQTIIIVEREMLQTAEKLGDEGSLTLLTDFISQQEKEIWMYNAWLN
                     Q"
     gene            12940..13485
                     /locus_tag="PLL59_03705"
     CDS             12940..13485
                     /locus_tag="PLL59_03705"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMY22941.1"
                     /translation="MALLSVGQQFPAFEKTAVVSLEKGKEFQTITNDIHQKDGKWLVI
                     FFWPKDFTFICPTEIAEFGKHNGDFKDRDAVLIGASTDSEYVHHAWRVHHADLNGLPI
                     PMLADTSKSLSRDLGILTEGEEVCYRATYIVDPQGIVRWVSVNDLSVGRNVAEVIRVL
                     DALQTDELCPCNWQKGEATLS"
     gene            13576..14151
                     /locus_tag="PLL59_03710"
     CDS             13576..14151
                     /locus_tag="PLL59_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMY22942.1"
                     /translation="MHETTQEILAELGIANDYTNATLQHLSLADSKYLRDLKLNVKAV
                     LTSEHLSAKETHLIALAIAANNNKEILVNSFAAKAKTNGASDAEIADAQACASLLAAN
                     NVLYRFRHFVEKEVYNNAPAKLRMNIMMNPVLGKEFFELISLAVSAVNGCEMCVKSHE
                     QSVLHAGGTENRIWDAIRIASIITSLGKAIY"
     gene            14245..14844
                     /locus_tag="PLL59_03715"
     CDS             14245..14844
                     /locus_tag="PLL59_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22943.1"
                     /translation="MLKPNMITTYKWYKYNLNVSCDKCNHPIPFQSFLGNPTCSECGN
                     IADKTWVEAVQFSNIAKVKQYSEGSTQLAGFMQLQMNYNAVENINCFHCHHILETPIN
                     TAIAVVNCKNCMLQISFEKQDNEALKNLVFYFHSNEKEKISGNVISLHCVSCGAPLQT
                     DTTKHEYNCNFCNTLNIIPPALRAKKILDDVYVGWNGKI"
     gene            complement(14860..19170)
                     /locus_tag="PLL59_03720"
     CDS             complement(14860..19170)
                     /locus_tag="PLL59_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMY22944.1"
                     /translation="MKNTIQILLFILFFISVKQSKAVCDFTIDKTTVCAGEVVTIQLK
                     PPYSKYHNIIVYKGVYPFGSVASSPADYSVLLPYDATKDNIIKITFRGDVVQQQYRIL
                     LAESNSDLIPVPNVPCNGGKVITVNPSPDPQLTETNNFVFCNAVTAQTVSISNASKTL
                     SINSNYSINWGDGSPVVNTATFNSPLTHNYAPGSYKITYTVTGSTPAPCNKATKVYNV
                     LIGKAPTISISASSPTVCIPANYTLPINLAQTSGVNPSSTLYKVFVNGELDTIYDNSN
                     LPALLQYTFTRGSCGNVSKDCNTDNKFSVRLYAVNECDVTDISQCIIVLDSIRPEIAG
                     PDTVCLREPTQYRNIDPFSRDDQCNIPPMTWTVTPNTGFTTSTPLGSPTFNNLNITFN
                     TKGTYFIKLKVKGACNEKDTSYKVIVIDRVNAQASFSTSACIPSTGFIDVPINNSSSD
                     TADIIKYIWTITPAAGVTFASGNANSKNVTVRFTKSGSYNVNLRVVGGCNEDTWDSTL
                     VIKGKPAIDTNKIPQACFVPFTFNPQTYFDYTNGGDNAATFTWTFTGAVPSSSTSQNP
                     GNIRYDLPGTYPIVLHISAQCGDSTLSNFITLNNNIRPNAGNDFGICKFDPPIQLSPV
                     PAGGVWRGQGITDSVQGIFKPSVVPTGTYNLAYVLNPTAACPTSDTLKVTVSEIIGLT
                     AGPDQSICKGIGPLQLVGNPQFSGGSWIGSGVVHSSLGIFDPAGLAPGNYQVGYVFQD
                     SLGACKDTAYKRITVFDSIHVALPPPTLCVNQAFNFGSITGNIANATWDFGDGTPNEF
                     IINPSHIYSRPGNFIVTLMAETPDRCRDTIKIPITVNSNPALTFIVSPDSSCSGNNIS
                     FTLPASHIPALNYIWDFGTSNVTTSTPSPQTFSFAKPLLKDTLYFVTLRADYNCGPVF
                     YTDTVKVKASPKADFGIQPIGCSPFTPTLANTSYGSPTDFFWNFGNGQTSTLQSPNPP
                     TYRNMTRRDSVITLSLRVSNVCGVDSVKKNITVKANDVFAKFFTSINQGCQPLDVEFF
                     NISSPGAEIIWDFGDGTSAYADNVTHEYDSAGVFRVKLLALGSCGRDSFFTTVNVFDK
                     PKPDFNVLTPCVNSNAQFINTTTNGNSYIWYFGDGDSSTSTRPNPTHVYNAIGVYNVK
                     LIVANSRPCVDSITKQIRVSEKPTAAFSVVNPQDCEQEPTVFLNSSSNASSYLWYLGN
                     GETSSIGGLSYTYPRAGIYNVTLSAVNGECRDSITKVAAVEIYPKPVADFLYEYTGDG
                     FNDPVEFTNITVNGQNYFWNFGDNDTSIVKDPTHQYNGDGPYRVTLFATSNKGCKDTV
                     SHPLGVDYAGQLYVPNVFSPEVGVGESAIWKPKGLSMKEYHVQVFSTYGQLLWESTAL
                     ENGQPTEAWDGRWKGTILPQDIYVWKIRAIFTDGTAWEGMKDPKTGKRAVMGSIMLLR
                     "
     gene            complement(19184..20263)
                     /locus_tag="PLL59_03725"
     CDS             complement(19184..20263)
                     /locus_tag="PLL59_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMY22945.1"
                     /translation="MRKLLKSVFLSFVLISVCYNLSLAQDPTFAQFYQFRTYLNPAAT
                     GSEKGLNVAMIYKNQWNYVPGGFNTYGIALDVQSARISSGVGVTAYRSVEAKTLQTNY
                     FSASYAYIARISKNINLHIGINGAFLNKSIDRSRLIFTDQLDPEVGVVPGGSSTNIVL
                     RKVNMFDLDAGVLLRFRYDIHKRPVHNSLGFAVHHLTRPDESFENISTKIPMRFTVTY
                     GAIFPVSRGKYKRSVDFYVSPVFKFDFQEKLRVYNAGMFATFKPIYAGAMYQFNKFSN
                     NNTHGLIFVGGIDWDLGKDNLMTLNVGYSYQLDFTGVTTKSRGQHEVTMRMNFNNVAL
                     LQPKKKKGKKIDCYDFPGKNAVKLF"
     gene            20402..20815
                     /locus_tag="PLL59_03730"
     CDS             20402..20815
                     /locus_tag="PLL59_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22946.1"
                     /translation="MLLSFFQTEEGVSFFSNLLNLVHLHLLFAVLFLISYTVKCILFL
                     SGKQEDFLNYKKKTLLVETLFSVGFLVFGFWVFIFHIKSGAYASMHWLDPKITLAIIG
                     IPLGIVGFKKENKKLVALSLAFFFVALIIGLMHYR"
     gene            20846..21250
                     /locus_tag="PLL59_03735"
     CDS             20846..21250
                     /locus_tag="PLL59_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HMY22947.1"
                     /translation="MKKINILLLLLSVSLSVAFANKTVNLSVKGMHCGGCEVKFKSTA
                     TKIDGVQEVSAVSAANSNATIIYDEKTTTEEKLIAALAEKTGYTIAVAANAGTTVAGK
                     PAGCCMSGKSNPSCKSADKQKCSKQKSKCTKD"
     gene            21406..23754
                     /locus_tag="PLL59_03740"
     CDS             21406..23754
                     /locus_tag="PLL59_03740"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HMY22948.1"
                     /translation="MNTLFYVVPFLGIFGLIITAIKYAWVKKQDAGDANMQELAGYIA
                     NGAIAFLKAEWKILFYFGVIAAILLAFLGWKGENSSPIIAIAFIIGAFFSAFAGYVGM
                     KVATLANVRTTQAARTSLKQALKVSFTGGTVMGLGVAGLAVFGLGGLFILFYFMFANG
                     ANVNSHEMLKAIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     VRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGQETISNDNFGGFGPILLP
                     MVIAGVGIVFSWLGTFFVSVKDDKGDVQGALNLGNWGSIVLTAVASFFLVKHILPDTM
                     ELRGHVFTSTGVFGAIMVGLVVGALMSIITEYYTAMGKRPVLSIVRQSSTGHATNIIG
                     GLAIGMESTVLPIIVLAAGIFGSYLCAGLYGVAIAAAGMMATTAMQLAIDAFGPIADN
                     AGGIAEMSQLPEEVRGRTDILDAVGNTTAATGKGFAIASAALTSLALFAAFVGVSGIS
                     AIDIYKAPVLAGLFVGGMIPFIFSALAISAVGRAAMDMVNEVRRQFREIPGIMEYKAK
                     PEYEKCVAISTQASIREMMLPGAIALITPILIGFLFGPEVLGGLLAGVTVSGVLMGMF
                     QSNAGGAWDNAKKSFEKGVDINGETFYKKSEPHKAAVTGDTVGDPFKDTSGPSMNILI
                     KLMSIVSLVIAPYIPSNGVVPVGNDSKNGHCTEMTSDACEAGKMENCAAEKNCTILSD
                     TTYTDSLGHTIVEKKCIVTEDKKEACCKEKASEKKACCAEKK"
     gene            23850..24470
                     /locus_tag="PLL59_03745"
     CDS             23850..24470
                     /locus_tag="PLL59_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HMY22949.1"
                     /translation="MRHLFMLLLLPNMLIAKEQKYLKSFFAYRQASYFATQVYQEHTV
                     ATFYKLKKINMPVNIDSLDIHLLNACLFFATNKLRLLHHKQALKIDFNLKNAAAIHSF
                     QMSNYHFFAHNHPYNSKLKTSAQRLKLCGITTAEAIGENCHKTSIYADLTYLELAQQV
                     IESFYQSPVHKQNLLNAQFRYLACATAIEFPNTDNAAIIVTQDFYD"
     gene            24572..24994
                     /locus_tag="PLL59_03750"
     CDS             24572..24994
                     /locus_tag="PLL59_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22950.1"
                     /translation="MRSIFLVLMCLFGLSLFAAQSSMIERYKPKNYSITCVQAYLNKE
                     PINTGTFLAKNSKGIITVSKGQNKNVLFNITLKSNIGKKDEPPIYLRFIKQKQLNISD
                     ILEYATEGDEILIEEFVPSINKIDLNCTPASITVVENL"
     gene            25038..28436
                     /gene="mfd"
                     /locus_tag="PLL59_03755"
     CDS             25038..28436
                     /gene="mfd"
                     /locus_tag="PLL59_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HMY22951.1"
                     /translation="MDLQQLLFLFRDDKRTQELLQHLQTSQARVLLRGTIGSSVNFIT
                     SSLFLQTDFTQLIIANDADEAQYIQNDIQNILEKKEILFLPSSYKKTNAFVEQSNHQI
                     LLRAQTLNALLNAKKGGEIIVTYPEALQELLVRKEKLIENTLFLKTGEKINIDFMLDV
                     LLEYGFNRTDFVYEPGEFSIRGGIIDVFSFGNEFPYRIELFDDEVESLRTFEPETQLS
                     LRKISELTIIPNIQSHFTKETTASLFEFLPNNCLLWLRDATMFSELTAKQYQLAIDEF
                     DIIANKKIKEHPFLHKTIHELFLAPNELMHEVAKCRIIELGNKSNDDWSNDTSTIINY
                     QQTPQASFNRNFDLLIQDMQAQQKKEISCFLFAENARQIERFHHIFEDKKANVRFHPC
                     YIDLAQGFIDKDLQIACYTDHQIFDRYHKYKTKAGFNRNKAITIRQLKDLNPGDYVTH
                     IDHGIGKFSGLEKITVNGQSQEMVRLIYKDNDLLYVNINSLHKISKYTGKEGHEPKIN
                     KLGSDAWTNLKNKTKKKIKDIAVELIKLYAQRKVAKGFAFNRDTYLQDELEASFMYED
                     TPDQSKATAEVKADMEKPHPMDRLICGDVGFGKTEIAIRAAFKAVADRKQVAVLVPTT
                     ILAWQHFKTFDARLKEHGVAVDYLNRFKSAKERKETLEKTKEGKIDILIGTHALLNKA
                     LEFKDLGLLIIDEEQKFGVSAKEKLRQFSANVDTLTLTATPIPRTLKFSLMGARDLSN
                     IMTPPANRIPITTEVQVFDVKNLKEIIEFEVFRGGQVYFVHNRVKDLVELETILRRLC
                     PEVSIRTAHGQLDGDQLEEIMLQFINGEFDVLLSTNIVESGLDIPNANTIIINNAHHF
                     GLSDLHQLRGRVGRSNKKAFCYLLSPPKSTLTDEARKRLQTLEEFSDLGSGFQIALRD
                     MDIRGAGNLLGGEQSGFITDIGFEMYHKILDEAIQELKYTEFKDVFKEQIEEQKQFVY
                     DCTIDTDVEMLIPPDYVQNTEERLRLYTELDAIENEEKLKAFAKKIEDRFGKLPKQVN
                     ELFDGLRIRWIAKKIGFERIILKNGKLRCYFLDNPKSPYYESPYFPRVMEHIQNSKKK
                     CTLKQSGNSLILVYDNIKTMHETESIFKEVDEKVFGKSIVEKIE"
     gene            28651..28869
                     /locus_tag="PLL59_03760"
     CDS             28651..28869
                     /locus_tag="PLL59_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22952.1"
                     /translation="MQTIKIEINNDAVLKLIEELETNKFIKIIKDKKKTKSSKKLSAL
                     LLGSINNKEAAKMKAELKNMRKEWERTI"
     gene            28851..29069
                     /locus_tag="PLL59_03765"
     CDS             28851..29069
                     /locus_tag="PLL59_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22953.1"
                     /translation="MGKNYLIDTNVIIDFAQNKLPRQSVSFLIDIIDNEPKISIINKI
                     ELLGFANTIKPIIEFVNASIIYDLDDAV"
BASE COUNT         9347 a         5046 c         5233 g         9615 t
ORIGIN      
        1 tttttgcttg cagaatgctg ttttttgttg gatagacgtc atctgcttca aacaaataag
       61 aaggaaattg tatgctgata tctttgtttt caaatgtttt tggttctttg ctatagcgac
      121 aagcggtaaa gaggaatagt gcgcaaagca tcgaccagag gccatatttt ttgaaattta
      181 tttgatgtaa catgttgcaa agttatgaaa ttaaaagtca attttgctgc cgtgttgttg
      241 tagccatttt ctgtgtgttt gataggcagg catggcgttt tctacatgtt tccaaaattt
      301 tggtgaatgg ttcatttctt tgaggtgcgc gagttcgtga acgattacat agtcgatgat
      361 ttcgcatggc agcaaaagca attttgtggc aaaggatagc ttgcgagaag aggaacagct
      421 gccccaacgt gacacggtgt gcttgatgtc gatcttttcg attttttctt tgaagtagcg
      481 ctcattccaa tagatggtgc gttcttggat tttagacaag aagtattttt ctgtaaagcg
      541 caacaagaag cgttggatat gatgtttttg ctcgacttcc ggaatgtttt gaagtaaata
      601 aatttttaga atatggtcgc cacgatagct gagtttgttt ctgccgctgg caatatattc
      661 tagttctatg gtaaatggag tgtcaaaaat atggagttgt ttattgtgat aaacctcaat
      721 tttattttgg tgaaattggt agtagttatt gtcttgtatt tgttgttttg cccacatcag
      781 aaattgtttg acaatctttt ctttatcagc aacagaaata tgtttaggca gtcgtacgat
      841 tacttcattt ttacctaagg acacacgtga tgagcgtcgt gcttccacga taatttttac
      901 tggcactggc aatgctgctg aattgtaaat attaatgctc atacgacaaa ataagcgtgt
      961 tatttttgtt tgtaatctta cgtctttttt cctaaaaact tacacacttt ttacaaacat
     1021 attttgagca gcgtcgttac tgatggtaat ttgtcttttg tcatttacaa taatttgcat
     1081 ggtttcgtca aactcttctt tttcgataat ttttatcttg tcgttgagtt gtaattgaat
     1141 tttatttaag tattttaaga atgatgctga gtcatcattt acattttgca gtatatataa
     1201 ttttttgagt ttagcatctg ttaaacaaat tacgtttggc accagaatat tgccttgctt
     1261 atccggaatt ggatcgccat gtgggtcaaa ttttggatat cctaaatatt catccatttt
     1321 atcgactagt tcatcagatt gtatgtgttc taattgctcg gcaatatcgt gaattttgtc
     1381 ccaggtaaat ttcaaatttt tggctaagaa tgtttcccat aagcgatgtt tgcgcatgac
     1441 ttgtatggca atttgctgtc ctatttttgt caatgaaaca ccataatact ttttatagct
     1501 caccagtttt ttatcggata ggcgtttgag catatcggtc actgatgctg ctgagtgttc
     1561 cagcttgtag gctaaatcat tggttttgac aattccattt tgcgtctcgc ctagttgata
     1621 tatatatttc agataatttt cttctactgc acttaagtta ttcatggtaa taattttaag
     1681 ctaatctaaa aataatattg ttatcttaaa aattaatatt ttaacattgc actaaattat
     1741 gtggaataat catagcaatt ggaaattcga acgaacgaca aactcattgc cagaagtgtt
     1801 ttcgagcatt gatgtgccca aaaatgtaca aggctggcgt aagcttttag cattcactgg
     1861 tcctggttta atggtagctg ttggctacat ggatcctgga aattgggcga ccgatattgc
     1921 tggtggttcg agctttggct atactttatt gtctgttatt ttaatatcga atttatttgc
     1981 cattttatta cagcatttat gtgtaaaatt aggtgttgtt accggcagag atttggcaca
     2041 agcttgtaaa gattcctatt caaaacctgt aagctttcta ttgtggatta tctgcgagat
     2101 tgccattgct gcatgtgatt tggcagaagt gataggctcg gcaattgcca tcaacttatt
     2161 attcggcata ccattaacgg ttggcatcat cattaccata ttagatgtat tggttttatt
     2221 gatgttgcaa aacaaaggat ttcgttattt agaggcgttc gtcggcagtt taatatttat
     2281 catattctgt tgttttgcgt atgaattatt tatggcaaag cctgcaattg cagcgttcat
     2341 gaatggtctt ttgcctacga cagaaatact caccaatgct gacatgttgt atatcgctat
     2401 tggaatatta ggtgcaacgg tcatgcctca taatttatat ttacattcta gcattgtgca
     2461 aacacgtaat tttgaacgca atgacgaagg caaaaagatg gccataaagt atgccactat
     2521 cgactcgact gcttcattgc taatggcctt ttttatcaat gctgctattt taattttggc
     2581 tgcttctgca tttcattaca atggccacaa agaagtagcc gacattcatg acgcatacaa
     2641 acttttagag cctgtgcttg gcgtaaaaat ggcttctact ttgtttgcta tcgcattgct
     2701 ggcaagtggt caaaactcta cgcttactgg cacactcgct ggacaaattg taatggaagg
     2761 ttttttaaat atccgtttaa agccttggct tcggcgattg attacacgct tgattgctgt
     2821 tgttccagca ctactcgttt caatattata tggcgagaat ggcaccggaa aattattagt
     2881 gctcagtcag gtgatattgt ctgtacagct cagttttgct gtcataccct tattacaaat
     2941 tacgagcaat aaaacgaaga tggcgcagtt tgccaattct gcttttttac actatacggc
     3001 ttggattatt gcactcatta tcatcatttt taatgctgtt atgttgtgga atatgttttt
     3061 tgcttaattt ttatagcatt aatacacatc ataattttcg accacataag tgccaagaat
     3121 acttttgcgc aattgcaatg tgatgtgttt attgggtgtt gaaagcgatt tgatttttgc
     3181 cattgtgctt ggccttactt tcattcttat agatttgttt tggaaagaaa atgagatatg
     3241 ttttttagtt tgatttttta aaccaataga ataaaatgta attggacaaa cagcggtgat
     3301 aatttcattt ttctttgctt gttgttgtat aacaaaatta atcggcaatc gaaaagccga
     3361 caaaagcaca aaggcgagca cgatagctaa aacgccatta aaaatgcttg acaccttttt
     3421 gtcttccttt ttttcagatg aaaatatttt ccattgagag caataaaatc cggtaattgc
     3481 taaggaaaca acataaagta ttgtgtttag ccaaaatgga aacaatatta ttttattgct
     3541 catgcttcgc gatatcaaaa caataataag cgaaacaatg acggtaataa gcactcgctt
     3601 tagtggaatc ttgttcattc ttttcttagc aatttataca attttatgcc taacatcaag
     3661 agtataacta aaatgaataa accaataagg ccagtaagaa agcttaagtt ggtttttact
     3721 acgactaaaa aaacaatggc aaacaaaaag attgttgcta cttcattcca aatacgcaat
     3781 tgtgttgatg tgtatttaaa aatatttttt tgttgttgtg aaaatatgcg atgcaaggaa
     3841 aagtgatata catataaacc gaggacaaac gctagcttaa tatgcatcca agtttgcgat
     3901 aaaaatccag attgtatcaa tagcaacaca ccaaaaatca aagtaattat ggcggctggc
     3961 catgtgatga tataccataa ttttctttgc ataattttaa attgtgcaat caatatggac
     4021 ttttctggtt ccattttatc atttgcctct gctgtataaa taaacaaacg tggcatataa
     4081 aaaagccctg caaaccaatt gactacaaaa atgatatgta gtgcttttat gtaattataa
     4141 tctaacacaa aacgaattta cgatttacga tttattttta aattactttt gatataaatg
     4201 taaaataaaa gcatttagat aaccaataca gcctataaaa taaaaatgtc tcaccatagg
     4261 cgagacattt tataaatatg ctttaattac tactttactt taactgtagt ttcttcgttt
     4321 atccagcatt ttactgtgta aggtccacca actgttagtt tttttgccgt gatttctgct
     4381 tttgtcggca aatatttgct atagtcgctg atgtattttt gtgcttcggt tactaagtct
     4441 gcgtctgcat ttatttcgat tactttatta aaataatcaa aagccggcca caatactatt
     4501 tgtgattgga agccagttcc agggccgcac aatggcccac tcgacaaata caataggccg
     4561 attaagttat atgctctact gtgtaatgga ttgtatttaa tagcttcttt cgcaaaatct
     4621 ctagacgcag gaaaatcttc ttttgcatat ttcataaatg aaatgtagta gttcaaatct
     4681 gcttttttca aagtgtctga ttctacagct aatgcatttt gatataagac gattgccgtg
     4741 tcatacattt tgtatttgat gtagatattt gccaaacgtg ttgcaaacgt ataatttggc
     4801 gctaacttgt ttaatttttc taacagctct acatttgttg tactgtctgc acaacctttt
     4861 gtttttaaat aaactgattt tatagtagct aaatcgtttg gatttgcttt gtattttttc
     4921 aaataaattt ctaacaattt agcacaatct tccggattgc ttttgtcggc aaagtttgtt
     4981 gtgtataaat cgtcaataac agattttacc tctttgtatt ctttattata cttgctactt
     5041 gtattggcaa tatttttatc tacaatagtc gttatttcat cgtatttatt gattgcatac
     5101 tcttccgtaa tgccattttt tccaacttgg tttactaata ctttgaaata cgtttgcaca
     5161 tagtagtaga acgctttttc gccagttatt tttatatatt tgtctaacaa agacagtact
     5221 tcaggcaaat ctgtattttt accatatttc tgcaaatcca ttgctttttt acctaacaca
     5281 tattcgtcat cgccaaaaca ttctatttgt ttattataaa tttgaaataa ggtatcgatg
     5341 tatgcctgac gcaatgtttt atcattttct ttttgaatca atgccgagta tatcgtgatg
     5401 ccgtctaagt gtgtttgttt tctaaaacct ggtgctttca aaaacatatt tctccattga
     5461 ggaagcgcat cagcatataa tcctttttta aagaactcac gatagataga atacgctggt
     5521 gttgttttgg tgctgtctcc atcaatgtaa cgtgcacagt tttcagcatt cgctactgac
     5581 cacgtcaata aaagcacatt tacaactaaa aagatttttt tcattttttt tctttgttta
     5641 tcaataatat tttcttctta caaaccattt atcatttaat gatattccga atttaagacg
     5701 ataaaagttt tcacgaacga ggtttttctg tgttgttcct ctgctgcctg cttcaaaccc
     5761 aatgttgaaa gcatacgtgt acactttttg taataatcct tgatcattag ctgtggttat
     5821 aatcaatgga atacctattc caaagtttat gccaaactca tttatagctt gattattgat
     5881 tttgatattt gtctgtgtgt agttaaatcc agcgcgatat ttcagccttg aaaagaactt
     5941 agaaaagttt ttatcattag gtaaaaactc tccaccaaca gctattctcc agctgttatt
     6001 cagttttgat gctgcgttgt cttcatagcc tctgtacttg ctccaaggtt gaaatttaaa
     6061 atcgatgcct gctctccatt ttataccacg ataggccatg ataccgatat tcaaagtagc
     6121 tggaatttta atgtaagcac gttcattaaa tttttctgac aatgtatcca acatatctgt
     6181 tgatgatact ttaggttgta tatagtctgt aataaaatcg tttataccag catcgcttgt
     6241 tcgataggct aaataacctg attggaatgt gtataatgct tcatcattat aggtgcgctt
     6301 gctaactttt gtaggtgcat ttcccgacaa gccaaaaacg acattataga ttttattgct
     6361 gtcctttttg ctcgttatcg gtaaattata ttgtacacct gcattccaaa ttatgctact
     6421 tacttttgtg ttggtaaaag tccatgttgt ataactttct gcatcaaatg tgcctacttt
     6481 attgattgga taagcaaaag aattgttgtt tagcttaccg aataaataac caagattaaa
     6541 accaacatga aaacctttgt atttatagcc agcgccccaa gataaattat acaaagagcc
     6601 tgtgccttca tattcaaatt ttactgcgtt tgctgtgtca aatgaagtgg tttgcgaaat
     6661 cagataatct tttgcagaaa agggcaataa gcttacgcct gtaacaaaga attttttgac
     6721 aggaaaaaac aaagacaaat aattcaagct aaaactattt tgctttgcct ttaaagtttg
     6781 tgtttttatt tttccgaaat tacctgagaa accgccgtca aatgttgtta attgcaatgc
     6841 ggcatacgaa gctggattga gataattgac tgtttcacca gactgaaagg cggcacccaa
     6901 tccgcccatt tgagctgatt gtgctgtatt attatcgaaa gcatgaccca atccgtagcg
     6961 tgaatatggt gtatgttctt gtgcatcaac ctgttgtaag gtaattgcta aaataaaaag
     7021 taaaaatatc ttacgcatta tattctagta tgtgattgag tccgattaat gtaaaattgg
     7081 aaattgcaaa tatctgattt ttaagcctat aaaccaacaa atccgaatcg ccacccgtaa
     7141 taattgtgtt catttcagaa aatttaacta aataatcact tatcataccg tcgatttctt
     7201 tgctcgctcc atacaaaaca ccgctttgta aattggcttc cgtattattt gccatcaggt
     7261 cagcatcgct aattaacgcc gcaagtggta attttccggt aaaagtatgc atggctttca
     7321 agcgcatttt caaacctgga tgaatgctgc caccgagaaa ttcattttgt gcattaataa
     7381 aattaaacgt tatgcaagtg ccacaaccga tgactaaagt attttggttt ggaaacattg
     7441 cagtggcggc ggctaccaat gctaatctat ctttacccag tgtttctttt gaagaatagc
     7501 agttgtgaaa tggcaatctt gtggtctcgt ttaatattaa atgctgtggc agtgatgcca
     7561 gtttttgtat tatataattt ggaatttcta ctgtgctgga aatgatagat ttgctaatat
     7621 tatgcgtagc aaatatttct tcgaatgcta ttttcttcga attgtcataa gtaattaatg
     7681 agcgcatttc ttgcttgtca aaaatgccga ttttcgttcg tgtattgcct atatctatcg
     7741 ctatattcat taattattag ctacttaatt tatccatttt acacaatctt tctgctgaca
     7801 gaattcgcca ttaaaatatt attttccata ttttcaatca acaccaaacc tggtgttttt
     7861 ccaacttgaa tagagcctaa aacgtcctca aaacctaatg ctttggcacc atttaaagtg
     7921 gcccaagtca atacagtttc aaaggaaaga tacgattgat atttcaaaat tgttttcatc
     7981 tcttccaata tattcaattg ccaattagac gttaaactat ccgtaccaat gcatacttgc
     8041 gaattattga caataaaatt attataattt ggcaagctgt tttcgatata caaatttgca
     8101 ttcggacaag tagcccaaaa ggtgtggtct gcaccaagtt gctgttgtgc cctagctata
     8161 tcctctttgg tgctgagcgt attgtgcaca aacaagtttc tatctgcttt attcaaaaaa
     8221 ttcaaggcat aatgaatggc tgattgacca atgtttggaa tcgcttctgt agtaaaacca
     8281 atgcgctcat aaaagtcaat gaaaccacct tttttatgta agaaaagctc attttctgcc
     8341 atggtttctt ggttgtgaat cgatattgtt ttgggcaaat acgcatcatc ttcattgttt
     8401 cttgcgataa tatgctgaaa caactttttc gaaactgaat atggcgcatg tggcactttt
     8461 gattttttat ttttatctgt ttctagtaat gcattgaata cagcatcgta ttgttcaatg
     8521 gtgtttgctg tattttgatc ttggaacaaa tcaaaatatt ccacaaaagt ataatagtgt
     8581 aaatgcgctt ttcttttttg tgaaaatgta tctgtcgtgt tagaaatatc gccgatagcc
     8641 actatgcctg attgtaacat attgttttct tcgcgtgcaa tggcgtcttg tattatttcc
     8701 tgtgaatggt ctctattttt gataacttgt gagatgaaat ctatcaagcc tgtgccggtt
     8761 tgtgctacac cttgcagatg tgataattca agatggcagt gtgcattaat aaaacctgga
     8821 acgatgacac cattaaaaaa ttcaactgtt gtagcgtcgt gcttgtccat gacatctaat
     8881 gctataattt ttccattttc atcaataata atcactccat tttcaatagg cgaagaggag
     8941 attggatata ttttatgtgc tgaaattttg cgaaatgaca tcgcaacaaa gatataaaac
     9001 tcaattgtca taagtcatta aaaatataaa gtatctttgc attagcttgc aaacacaaat
     9061 cgacatacga acattatcaa aagaggaatt gatagcacaa ttagcaccac ttggagagaa
     9121 gccgtttcgt gcaaaacaaa tctatgagtg gctatggaag aaatctgctg ttgattttga
     9181 tgaaatgacg aatctttcca aagatttgag agaaaagtta aagcaatact ttttgttgct
     9241 gccaatacga gcttataagg tgcaaaagtc aaatgatggc acgattaagt cggcatttca
     9301 actacatgac aagcatctaa ttgaaggtgt gttaattcct acggaagaca gagcaacggc
     9361 atgtgtttcc tcgcaagttg gttgtagttt gagctgttcg ttttgtgcta ctggcttttt
     9421 aaaaagagaa agaaatttat cggctgctga aatttatgat caagttgttt taatcaatca
     9481 acaagcaatt gaaagcatag gcagaaatct gtcgaacatc gtgtttatgg gcatgggcga
     9541 gccattgctg aattacaata atgtgatgtc tgccattgat aaaattacgg cagaagaggg
     9601 cttgaatatg tccgccaaac gcataacagt atcgactgcg ggcattgcta aaatgataaa
     9661 aaagcttgct gatgataatg ctcgtttcaa cttagcattg tcattacatg ctgctgatga
     9721 tgagaaacgc aataccatca tgcctattaa tgagcaaaat aatattgacg ctttaataga
     9781 tgcattgcaa tattttcagt caaagtgtaa aggtgaagta acatttgaat atattttgtt
     9841 agatggcgta aatgacagca tcgacgatgc aaaaaagcta gttgctattt gcaaaagatt
     9901 gatagatgta aaaatcaatg tgattgaata taataaaata gagcaagccg attttcataa
     9961 atctaccgtg gaaacacgag aaaaatttat tgactattta aacaagcata aagttattgc
    10021 caatgtgcgt aaaagcagag gaaaggatat tgatgccgcc tgtggtcaat tagcgaataa
    10081 atcctaaact atgatgattt ggtggttttc cttagtatag ctcgattata ctattcacat
    10141 tcattatcta ttgccgaaat attgtaatat ctttagatgg tttgcgactt atcttattcg
    10201 aatcaataca tttcatttgc aacaagcgtt tttacaaact attaatcaac atcaaggcat
    10261 catacataag gtgtgtagaa tttattgtaa taatcaagac gaccacaatg atttatttca
    10321 agaaattacg ctacaacttt ggaaagcatt tccttcattt agaagtgagg caaaaattag
    10381 cacatggatg tatagaatag cactaaacac tgctatttca ggaataagga agaaaaaaat
    10441 tcaaacaaca gcattagaaa atataaactt tcaaccagta gattttaatg acacgagcac
    10501 agaagataac atgctacttt tatacaaagc cattgcatat ttatctgacg tggaaaaatc
    10561 gattgtgtta ttatatttag aagataagcc atatgatgaa attgcagaaa taacaggcat
    10621 caccgcaaat tatgtggccg tgaaaatgaa ccgaattaaa gaaaaattaa gaaccttatt
    10681 aaagacagta caatgataga gctagatgat ttaaaaaata tttggtcgag caatatcgaa
    10741 catgatattg gaaaacaaac gctggaaaaa gaaaatatta aagcgctgtt gcgtcataaa
    10801 tctgtttcca taatcgacaa gttgcgcagg aatttattac tcgaaattgc tatgtttttt
    10861 gtatgtcttc tcttaatagc tggtgttcca ttttatttca aatctaaaga aattgcaatt
    10921 atctgtgtcg tcgtaatctt gtttaccttt gtgccttatt tattttatta cattaaaaaa
    10981 tacaaagaat tacaacgatt tttctcttat catgaaaata taaaatcaaa tttggaatta
    11041 ttgatagtgc aactcgaaaa gtatctaaat atttattttt ggggcagcct attgcttaca
    11101 ccaatttctg gatttttatc aggctatgct atcttatacg aactgaaagc actcggtttc
    11161 ttattgtatt tcgacatgtt caactctggc actttaacta taatattatc attcgcattg
    11221 ctgctcacat tattatccta tccaataatg aaatggtata tccgaaaatt ttatggacag
    11281 tatttagaga aattaaaaga ctgcttaaaa gagttgcaag aaattgaata gcttatttcc
    11341 attccacaac agtgcctcgc tttttatcta aaagcacatc tggaatattg tcaattatta
    11401 gtttagccaa caaatcaata aactttttct tcgcaacact gcgcgcatat acaatactga
    11461 cttctcttag tggttttggt tttttaaact ctgcgagttg cggtagtttt ttagtaggta
    11521 attcgagtac tgccagctct ggcaataaag taaaaccacc ttccatttct accatctttt
    11581 ttaatgtttc tatcgagcca ctttcgtatt tcaaacggtc gttaactcgc attttattcg
    11641 tgcaaatatt aatcacatga tttctaaaac aattgccatt cgatagcatc catatatcgt
    11701 ctctttgtat gtcttcatga cttaagtttt tcttcttagt taatggatga ttgtagtgcg
    11761 catacacctt tatttcttcg taaaacaaag gttcttcaat caaattaatt tcatgtaatg
    11821 gcgttgccaa tattgctgcg tcaagctcgt ctttattcaa tttaaaaaca atatcctccg
    11881 ttgtcagctc ttgtataatc aaacgaattt ttgaatattt tttaaggaaa tcactaataa
    11941 aataaggcag caaataaggt gcaatcgtcg gaataatgcc tatggtaaaa tcgccttcta
    12001 aactgtcgtc gtaatgcttg gcaatttgtt ctaatttttt cgcttcacgc aatacaatac
    12061 gcgcttgttc tatcaggagc ttacccgcat ctgtcggaat aataggctgt ttacttctgt
    12121 caaaaatatc aatatttagt tcctcttcca gcttttttaa ttgcatactc aaagttggtt
    12181 gtgtaaccaa acaagcttct gccgctttgg caaaatgtcg ataattgtct attgccaaaa
    12241 tatattctaa ttgtgttaat gagatgttca taagtattat ttattaagga ataaaattaa
    12301 ttgatttttt ttattaaaca atggttttta actttgttgt ataaaattaa taaacatgaa
    12361 aaaaacaaag catattacgc aaattggatt ggacactaaa caatctgaaa cacttgcaag
    12421 ccaattaaac gtcctattgg caaactatca attatattac caaaatgcgc gtggtttgca
    12481 ttggaatatt aaaggaaaac atttcttcca attgcataca aaatttgaag aaatatatac
    12541 agatgcgcaa gaaaaaatag atcttattgc tgaacgaatc cttacattag ggtttacacc
    12601 attgcatact tttagcgatt atctaaaaca cactactata aaagaaggaa aacaaataag
    12661 cgatgcaaat gaagcggtgg cattagtcat tagcggcctg caaacaatta ttattgtaga
    12721 acgcgaaatg ctgcaaacag cagaaaaatt gggcgatgag ggctcattga ccttacttac
    12781 agattttatt tcgcaacaag aaaaagaaat atggatgtac aacgcctggt tgaatcaata
    12841 gttttttatt attgcatcat aaaaactatc gattttattt ataaaaataa atgacgaact
    12901 ttacatcata aacaatttta aacaattaaa cacaaaacaa tggcattatt atcagtaggg
    12961 caacaattcc cagcattcga aaaaacagca gtagtaagct tagaaaaagg aaaagaattc
    13021 caaacaatca caaacgacat tcatcaaaaa gatggcaaat ggttagttat tttcttttgg
    13081 ccaaaagatt tcacattcat ctgcccaaca gaaattgctg aatttggcaa acacaatggc
    13141 gattttaaag atagagacgc tgtgttaatt ggcgcatcta cagatagcga atatgtacac
    13201 cacgcatgga gagtacacca cgctgatttg aacggcttac ctattccaat gttagcagat
    13261 acatcaaaat ctttatcaag agatctaggt atcttaacgg aaggtgaaga agtatgctat
    13321 cgcgctacat atatcgtaga tccacaaggt atcgtaagat gggtgagtgt aaacgatttg
    13381 tctgttggta gaaatgttgc tgaagttatt cgcgttttag atgctttaca aacagacgaa
    13441 ctttgtcctt gcaactggca aaaaggagaa gctactttat cataaataat tattcaaaaa
    13501 aaagtaaata agcgtcaaga ctttcttctt gattcttatt tattatattc aaaaaacatc
    13561 taacacattt acaacatgca cgaaacgaca caagaaatat tagcagaatt aggcattgca
    13621 aacgactata ctaatgcaac actacaacac ttatcccttg ctgacagtaa atatctacgc
    13681 gatttaaaat taaatgtgaa ggcagtgttg acatcagagc acttgtctgc aaaggaaact
    13741 catttaattg cactagcgat tgccgccaat aataacaaag aaattctggt gaactcattt
    13801 gctgcaaaag ccaaaaccaa cggtgcctca gatgctgaaa ttgctgacgc acaggcttgc
    13861 gcatcattac tcgctgcaaa taatgtattg tatcgttttc gtcattttgt agaaaaagaa
    13921 gtctataata atgcgcctgc taaattgcgc atgaacatca tgatgaatcc tgtgttgggc
    13981 aaagagtttt ttgagttaat tagcttggca gtaagcgcag taaatggttg cgaaatgtgt
    14041 gtaaaaagcc acgaacagtc agtattacat gcaggtggca cagaaaacag aatttgggat
    14101 gccatcagaa ttgcaagcat aatcacaagc ttaggcaagg caatctatta acaaaacaaa
    14161 cacaagtcta gatattaaaa actgaccgtt tagcacttct aaacggtctt ttttttgcat
    14221 gatgcttaag attttctatc ttgtatgtta aaaccaaaca tgattactac ttataaatgg
    14281 tataaatata atctcaatgt tagttgcgat aaatgcaatc atccaattcc ttttcaatct
    14341 tttctaggca atccaacttg cagtgaatgt ggcaatattg ctgataaaac ttgggtcgaa
    14401 gcggtgcaat tttctaacat tgcgaaagtg aaacaatata gtgaaggtag cacacaattg
    14461 gctggtttta tgcaattgca aatgaactat aatgccgttg aaaacattaa ctgctttcat
    14521 tgtcatcata tacttgaaac accaataaat acagccattg ctgttgttaa ttgtaaaaat
    14581 tgtatgcttc aaatttcgtt tgaaaaacaa gacaatgaag cattaaaaaa tcttgttttt
    14641 tactttcata gcaatgaaaa agaaaaaata agtggaaatg tcatttcgct gcattgtgtt
    14701 tcctgtggag caccactaca aaccgacacg acaaagcatg aatacaattg caatttttgc
    14761 aacacactca atattattcc gccagcacta cgcgctaaaa aaatattaga cgatgtatat
    14821 gttggttgga atggtaaaat ttaaatatcg ttacttcatt tatcttaata acataattga
    14881 acccatcacg gctcttttgc ctgttttagg gtctttcatg ccttcccacg ctgtaccatc
    14941 cgtgaaaatg gcgcggatct tccacacata aatgtcttgt ggtaatattg tgcctttcca
    15001 tctgccgtcc caagcttccg taggttgtcc gttttccaat gctgtgcttt cccaaagcaa
    15061 ttgaccataa gtagaaaaca cttgtacatg atattctttc atcgacaaac cttttggttt
    15121 ccaaattgca gactcaccga cgcctacttc tggagagaac acattcggca catataattg
    15181 accagcgtaa tcaacgccta aaggatgcga caccgtgtct ttacagcctt tattgctagt
    15241 agcaaacaag gtaactcgat agggtccgtc tccattatat tgatgggttg gatctttcac
    15301 tattgaagta tcattatcgc cgaaattcca aaaatagttt tgtccattca ccgtaatatt
    15361 ggtgaactca acagggtcat taaatccatc gccagtatat tcgtataaga aatctgcaac
    15421 tggttttgga tatatctcaa cagcagcaac ctttgtaata ctatcacgac attcaccatt
    15481 aacggcgcta agcgttacgt tatatattcc tgctcttgga taagtataag acaaaccacc
    15541 aatacttgac gtttcaccat ttcctaagta ccataaataa ctcgatgcat tggaagatga
    15601 atttaaaaag acggtaggtt cttgttcaca atcttgcgga ttgacaacac taaatgcagc
    15661 agtcggcttt tctgaaacac gaatctgttt ggtaatcgaa tctacacaag gtcggctatt
    15721 ggctacaatt aattttacat tatatacgcc aatcgcatta tatacatgtg taggatttgg
    15781 tcttgtagat gttgaactat caccatcgcc aaaataccaa atgtaactat tgccatttgt
    15841 cgttgtattt ataaattgag catttgaatt tacacaaggt gtaagcacgt tgaaatctgg
    15901 ttttggtttg tcaaacacat ttaccgttgt aaagaaagaa tctctgccac atgagcctag
    15961 tgccaataat ttcactctga aaacacctgc gctatcatat tcatgtgtca cattatccgc
    16021 ataggcagaa gtgccatcgc caaaatccca aataatttct gcacctggag acgagatatt
    16081 aaaaaactct acatctaatg gctggcagcc ttgattaatg cttgtaaaaa atttcgcaaa
    16141 tacatcattt gctttcactg taatgttttt cttcacgcta tcaacaccac aaacgttaga
    16201 cactctaagg cttaaagtaa ttacagaatc acgtctagtc atgtttctat aggtcggtgg
    16261 atttgggctt tgaagtgttg atgtttgtcc attgccaaaa ttccagaaaa agtctgttgg
    16321 agaaccatat gaagtgttcg ctagagttgg cgtaaacggc gaacagccta ttggttggat
    16381 tccaaaatct gcttttggag aggctttcac ttttacggtg tctgtgtaaa aaacagggcc
    16441 acaattataa tctgctcgta aggtgacaaa atataaagta tcttttagta gtggctttgc
    16501 aaacgaaaat gtctgaggag atggtgtaga agttgttaca ttacttgtgc caaaatccca
    16561 aatataattc aaagctggaa tatggcttgc tggtaaagta aagctgatat tattgccaga
    16621 acatgacgag tctggagata caataaatgt taatgctgga ttggaattta ccgttattgg
    16681 aattttgata gtatctctgc aacggtcagg tgtttctgcc attaatgtaa caataaaatt
    16741 acctggtctc gagtaaatat gtgatggatt gataataaac tcatttggtg tgccgtcgcc
    16801 aaaatcccat gttgcattgg caatatttcc agttattgaa ccaaaattaa atgcttgatt
    16861 tacacataat gtaggtggcg gcaaagcgac gtgaatactg tcaaacacgg taatgcgttt
    16921 ataagcagta tccttacatg caccaagtga atcttggaaa acataaccaa cttgatagtt
    16981 acctggcgct aagcctgctg ggtcgaaaat tcctaagcta gaatgaacaa cacctgagcc
    17041 tatccaactg cctccactga actgtggatt acctactaat tgcaatgggc ctatgccttt
    17101 acaaatactt tggtctggcc ccgcagttaa acctataatt tctgacacgg tcacttttag
    17161 cgtgtctgag gtagggcaag ctgctgtcgg atttaataca taagcaagat tatacgttcc
    17221 tgttggcacc actgatggtt taaaaatacc ttgaacagaa tctgtaatgc cttgtccacg
    17281 ccaaacacca cctgccggca ctggcgatag ttgtatgggc ggatcaaatt tacagattcc
    17341 aaaatcattg cccgcattcg gtcgtatatt gttatttaaa gtaataaaat tacttaatgt
    17401 agaatcaccg cattgtgcac taatatgcaa tacaattgga tatgttcctg gtaagtcata
    17461 gcgaatattt ccaggatttt gagaagtcga tgagcttgga acagctcctg taaatgtcca
    17521 tgtaaatgta gcggcattat cgcctccatt cgtataatca aaataagttt gtggattaaa
    17581 ggtgaaagga acaaaacaag cttgcggaat tttattggta tctattgctg gttttccttt
    17641 aataacgagc gtactgtccc aagtatcttc attacaacca ccaacgacac gaaggtttac
    17701 attgtaagat cctgatttag tgaatcgaac ggttacattt tttgagttgg catttcctga
    17761 agcaaaagtt acacctgccg caggcgttat tgtccaaata tattttataa tatctgctgt
    17821 atctgacgaa ctattgttaa tcggtacatc aataaaacct gttgatggaa tacatgctga
    17881 cgtactaaaa gaggcttgtg catttaccct atcaataaca attactttat agcttgtatc
    17941 tttttcatta caagctcctt ttacttttaa cttgataaaa taggtgcctt ttgtattgaa
    18001 ggtaatattt aaattattaa aagttggcga acccaatggc gtgctggtgg taaatcctgt
    18061 gttgggtgtt actgtccaag tcattggcgg tatattacat tgatcgtctc tgctaaatgg
    18121 atcgatgttt ctgtattgtg taggctcgcg cagacagact gtatccggac ctgcaatctc
    18181 tggtcgaatg ctgtctaata caataataca ctggctaata tcagtgacat cgcattcatt
    18241 tactgcatac aatcgaacag aaaatttatt gtctgtatta caatctttgg aaacattacc
    18301 acaacttcct ctcgtaaatg tatattgtaa taatgctggt aaattactat tgtcataaat
    18361 agtatctaat tcaccattaa caaaaacctt atacagtgta cttgatggat ttacgccaga
    18421 tgtttgtgct aaatttattg gaagtgtata attagcagga atacataccg ttggcgatga
    18481 tgctgatatt gaaattgtag gtgctttacc gattaacaca ttatatactt ttgttgcttt
    18541 attacaaggt gctggcgtag aaccagtaac cgtataagta attttatagc tacctggtgc
    18601 atagttgtgc gtcaatgggc tgttaaatgt agcagtgttt acaacaggac tgccatcgcc
    18661 ccagtttata ctgtaattgc tattgatgct taatgtttta gaggcattgc ttattgatac
    18721 ggtttgtgca gttacggcat tacaaaaaac gaaattattt gtttccgtta attgtgggtc
    18781 aggtgaagga tttacagtaa tgactttacc gccattacaa ggcacattcg gaaccggaat
    18841 taaatcggag ttgctttctg ctaataatat tctatactgc tgttggacaa catcacctct
    18901 aaatgtaatt ttgatgatat tatcttttgt cgcatcataa ggtaataaca cactataatc
    18961 agcaggagag ctggccacac taccaaacgg ataaacacct ttatacacaa taatattgtg
    19021 atattttgaa tagggaggtt ttagctgaat ggtaacaacc tcgcctgcgc agaccgttgt
    19081 tttgtctatc gtaaaatcac aaactgcttt actttgtttt acgcttatga aaaacaagat
    19141 aaatagtagt atttgtatag tatttttcat gatttattta aagttaaaac aatttcacag
    19201 cattttttcc tggaaaatcg tagcaatcaa ttttctttcc tttctttttc ttaggttgta
    19261 ataaggctac attattaaag ttcatgcgca tcgtcacttc gtgttgtcct ctactttttg
    19321 ttgtaacacc agtaaaatct aactgatagc tatatcctac atttaatgtc atcaaattgt
    19381 ctttacctaa atcccaatct atgccgccga caaaaatcaa accatgtgtg ttattattgc
    19441 tgaatttatt aaactgatac atagcacctg catatattgg tttaaaagta gcaaacatac
    19501 ccgcattata tactcgtaat ttttcttgaa aatcaaactt aaacacaggc gacacataaa
    19561 aatctacact gcgtttgtat ttgcctcttg acactggaaa tatggcgcca taagttacag
    19621 taaaacgcat gggaattttt gtcgaaatat tctcaaaaga ctcatcagga cgcgttagat
    19681 gatgcaccgc aaaccctaaa gaattatgca ctggccgttt atgtatatca tatcggaaac
    19741 gcaataacac gcctgcgtct aaatcaaaca tgtttacttt gcgtaaaacg atattcgtgc
    19801 tcgagccacc aggcaccaca cctacttctg ggtctaattg gtctgtgaaa ataagcctgc
    19861 ttctatcaat agatttattt aaaaaagcac cattaatacc gatatgtaag tttatattct
    19921 tagaaatcct agcaatgtag gcatatgatg cggaaaaata gttggtttgc aatgtcttag
    19981 cctccacact tctataagca gttacaccaa cgccagaaga gattcttgct gattgcacat
    20041 ctaaggcaat gccatacgta ttaaaaccac ctggcacata attccattga tttttataaa
    20101 tcatcgccac atttaggcct ttttcagaac ctgttgctgc cggatttaaa tacgttctaa
    20161 actgatagaa ctgtgcaaat gttggatcct gagctaagga caaattatag catacgctaa
    20221 taagaacaaa gcttaaaaat acactcttta ataattttct catttctgct gtgataacgg
    20281 cttatctcta ttggattgac acagaatggc tttcttatac aaaagtagaa taaatagttc
    20341 ataacttctt ctttttgagg ataaaagttt gaaagtgtgt atctttgtaa aaattattaa
    20401 tatgctatta tcatttttcc aaaccgaaga aggtgtttcg ttttttagca acctactcaa
    20461 tctagtccat ttacatcttt tatttgccgt gttgtttttg atttcttaca cagtaaaatg
    20521 cattctattt ctttctggta aacaagaaga ctttctgaat tataaaaaga aaaccttatt
    20581 ggtagaaacc ttattttctg taggattctt ggtgtttgga ttttgggtgt ttatttttca
    20641 tataaaatct ggcgcttacg cttccatgca ttggctcgac cctaaaatca cattagccat
    20701 tattggcatt cctttaggta ttgttggatt taaaaaagaa aataaaaaac tagttgcttt
    20761 gtcactagcc ttcttttttg ttgccttaat tattggattg atgcattata gataataact
    20821 ttgtttagta taaaatatta aaaacatgaa aaagattaat attctcctgc tattattaag
    20881 tgttagcctt tccgttgctt tcgccaataa aacagtaaac ttatctgtaa aaggaatgca
    20941 ttgtggtggc tgtgaagtca aattcaaatc aactgccact aagattgatg gcgttcaaga
    21001 ggtaagtgct gttagtgctg ccaacagcaa cgcaacaatc atctacgatg agaaaaccac
    21061 aacagaagaa aaacttatcg ctgctctggc agaaaaaaca ggttatacca ttgcagttgc
    21121 tgctaatgct ggcactacag tggcaggcaa accagcagga tgttgcatga gcggcaaaag
    21181 caatccctct tgcaaatctg cagataaaca aaaatgcagc aagcagaaat ctaaatgtac
    21241 taaagactga ttttacggtg taaatcagcc ttttactaat taaaaagacc aaatagtaca
    21301 caatctattt ggttttttat tttttatgtt aacaattgcc agaattgtta ataattctaa
    21361 actttgttgt acttttgcac cgctttaaaa atcaaattat tagatatgaa tacacttttt
    21421 tacgtagttc catttcttgg aatttttggt ttgataatca cagcaattaa atatgcttgg
    21481 gttaaaaaac aagatgcagg cgatgcaaac atgcaagagt tggctggtta tattgccaat
    21541 ggtgccattg catttttaaa agcagaatgg aaaatcttat tctattttgg cgttatcgct
    21601 gctattttat tagcattttt aggttggaaa ggggaaaact ctagtccaat tattgcaatc
    21661 gcatttatta tcggtgcttt tttctcagcg tttgctggtt atgtaggtat gaaggttgcg
    21721 acattggcaa acgtgcgtac gacacaagcg gcacgtacaa gcttaaaaca agcattaaaa
    21781 gtatcgttta caggcggaac tgtcatgggt ttaggtgttg ctggcttggc tgtttttggt
    21841 cttggtggtt tgtttatcct attctatttt atgtttgcta atggagccaa tgtaaatagc
    21901 cacgaaatgc ttaaagcaat cgaggtatta acaggttttt cattaggcgc tgagtctatt
    21961 gcattgtttg cacgtgtagg tggtggtata tatacaaaag ctgctgacgt tggcgctgac
    22021 ttagttggca aagtggaggc tggtattcca gaagatgatg ttagaaatcc ggctacaatt
    22081 gctgataatg ttggtgataa tgttggcgac gttgcaggta tgggagccga tttatttggt
    22141 tcttatgtgg caactgtttt agcaacaatg gtattaggtc aagaaacaat atcaaacgat
    22201 aactttggtg gttttggtcc aatattatta ccaatggtca ttgctggtgt aggtatcgtt
    22261 ttctcttggt taggaacatt ctttgtgtct gtaaaagatg acaaaggcga tgttcaaggt
    22321 gcattaaact taggaaactg gggttcaatc gtattaacgg cagtagcttc tttcttctta
    22381 gtaaaacata ttttaccaga tacaatggaa ttacgtggcc atgtatttac atctactggt
    22441 gtgtttggcg ctatcatggt aggtttagta gtaggtgcat taatgtctat cattacagaa
    22501 tattatactg caatgggcaa acgtccagta ttgtcaattg tacgtcaatc ttctacaggt
    22561 catgccacta atatcattgg tggtttagca ataggaatgg aaagtactgt gttgcctatt
    22621 atcgtattgg ctgcaggtat ttttggttcg tatttatgtg ccggtttata cggtgtggct
    22681 attgcagcgg caggtatgat ggcaacaact gctatgcaat tagcaatcga tgcattcggt
    22741 ccaattgctg ataatgcagg tggtatcgcc gagatgagcc aattaccaga agaagtgcgc
    22801 ggcagaacag atatcttaga cgctgttggt aatacaacag ccgctacagg taaaggcttt
    22861 gcaatcgctt ctgctgcttt aacttcatta gcattatttg cagcattcgt tggtgtttct
    22921 ggcatctctg caattgatat ttacaaagca ccagtactag caggtttgtt tgttggtggt
    22981 atgattccat tcatcttctc tgctttagct atttctgctg ttggtcgtgc ggcaatggat
    23041 atggtgaacg aagtacgtcg tcaattccgc gaaattcctg gtatcatgga atataaagca
    23101 aaaccagaat atgaaaaatg tgtggctatt tccactcaag cttctattcg cgagatgatg
    23161 ttgccaggtg caatcgcatt aatcacacct atacttatcg gcttcctatt tggacctgaa
    23221 gtgttaggtg gtttattggc tggtgttacg gtttctggtg tgttaatggg catgttccaa
    23281 tcaaacgctg gtggcgcttg ggacaatgct aaaaaatctt tcgagaaagg tgtggacatc
    23341 aacggcgaaa cattctacaa aaaatctgag ccacataaag cggctgtaac aggcgacacc
    23401 gtaggtgatc cattcaaaga tacttctggt ccatcaatga atatcctaat caaattgatg
    23461 tctattgtat ctttagttat tgctccttat attccatcta atggtgttgt tccagttgga
    23521 aatgacagca aaaacggaca ctgcacagaa atgacaagcg atgcttgtga ggctggcaaa
    23581 atggaaaatt gcgcagcaga aaaaaattgt accattttaa gtgatactac atataccgat
    23641 tctttaggtc atactatcgt agaaaaaaaa tgtattgtaa cagaagataa aaaagaagcg
    23701 tgctgcaaag aaaaagcaag tgaaaaaaaa gcttgttgcg ctgaaaaaaa ataaaaatat
    23761 acgtactacg tcttataaaa gctccgactc gttcggagct ttttttatat cacataaaat
    23821 aaaaattgca caattgcgta ctatactgta tgcgtcattt atttatgctg ttgttattgc
    23881 ccaatatgct gattgctaag gagcagaaat atttgaaatc atttttcgct tatcggcaag
    23941 catcttattt tgctacgcaa gtttatcaag agcataccgt tgccacattt tataagttaa
    24001 agaaaataaa catgcctgtc aacatcgata gtcttgatat tcacttatta aatgcttgtc
    24061 tgttctttgc tacgaataaa ttgcgattat tacatcacaa acaagcattg aaaatagatt
    24121 ttaatttaaa aaatgcagcc gcaatacata gttttcaaat gagcaattac catttttttg
    24181 cgcataatca tccatataat tccaagttaa aaacaagtgc tcagcgccta aaattgtgtg
    24241 gcattacgac tgctgaggca attggcgaaa attgccataa aacaagtatc tatgccgact
    24301 tgacttatct cgaactagca caacaagtca tagaatcttt ctatcaatcg ccagtacaca
    24361 aacaaaactt attgaatgca caatttcgat atcttgcttg tgcaacagca atagaatttc
    24421 caaacacaga caatgccgcc attatcgtga cacaagattt ttacgactaa gcttgaattt
    24481 ctttttataa taaatgaact tttaatgcgt ttttaacatt aatctgttat gtattttaac
    24541 aaatgcgacc aattttttat aaatttatat aatgcgaagt atattcttag ttttgatgtg
    24601 tttgtttggc ttgtctttat ttgctgctca aagtagtatg attgaaagat ataagcctaa
    24661 aaactatagt attacgtgcg tgcaagctta tttaaacaag gagcctatca acacaggcac
    24721 ttttttggca aaaaattcaa aaggcatcat caccgtttct aaaggacaaa ataaaaacgt
    24781 actattcaat attaccttga aatcaaatat tggcaaaaaa gatgaacctc caatttatct
    24841 gcgttttatc aaacaaaagc aactcaatat ttctgatatt ttagaatatg ccacagaagg
    24901 tgatgaaatt ttgattgagg aatttgtgcc atctattaat aaaattgatt taaactgcac
    24961 acctgcgagt attactgtcg tagagaattt ataattcact ttcttttcaa ctatctaagc
    25021 tttatctttg catcgcaatg gatttgcaac aactattgtt tctgtttcgc gatgacaagc
    25081 gcacgcagga actactacaa catcttcaaa cttcgcaggc acgcgtactg ttgcgtggaa
    25141 ctattggctc ttctgtaaat tttataacgt cttccttatt tttacaaact gattttacgc
    25201 agctgattat tgccaacgat gccgacgaag cacagtatat tcagaacgat atacaaaaca
    25261 ttctagaaaa aaaggaaatc ttatttcttc catcatctta caagaaaaca aacgcttttg
    25321 tcgagcaaag caatcaccaa attttgttgc gtgcacaaac gttaaacgca ttattaaatg
    25381 ccaaaaaagg tggtgaaata atagttacct atccagaagc tttgcaggaa ttactcgtca
    25441 gaaaagaaaa gctcattgaa aatacactct tcctaaagac tggtgaaaaa ataaacatcg
    25501 attttatgct cgatgtatta cttgaatacg gttttaatcg aaccgacttt gtatatgagc
    25561 ctggcgaatt ttctatccgt ggaggcatca tcgatgtgtt ttcttttggc aacgaatttc
    25621 cgtatcgtat cgagctgttt gacgatgaag tggaaagctt gcgcaccttc gagccagaaa
    25681 cgcagctctc gctacgcaaa atttctgaac ttaccataat accaaacata caatctcact
    25741 ttactaaaga aaccactgct tctttatttg aatttttacc taataattgt ttgttgtggc
    25801 tgcgcgatgc tacgatgttt agtgagctaa cggcaaaaca atatcaactt gcaatagatg
    25861 agtttgatat catcgccaac aaaaaaatta aagagcatcc ttttttacat aaaacgatac
    25921 atgaattatt tcttgcacca aacgaattaa tgcatgaagt agctaaatgt agaattatag
    25981 aattaggaaa taaaagtaat gacgattgga gcaatgacac ttcgacaatt atcaactatc
    26041 agcaaacacc acaagcttct ttcaatcgca attttgattt attaattcaa gatatgcaag
    26101 cgcaacaaaa aaaggagatt tcttgttttt tgtttgctga aaatgctcga caaatagaac
    26161 gtttccatca catcttcgaa gataaaaaag ccaatgttcg ctttcatcct tgttatatcg
    26221 atttggcgca aggttttatc gataaagact tgcaaatcgc ttgttatact gaccatcaga
    26281 tttttgatcg ctatcataaa tacaaaacca aagctggctt taaccgaaac aaagctatta
    26341 ccattcgaca attaaaagac ttgaatcctg gcgattacgt tacacatatc gaccacggta
    26401 tcggcaagtt ttctggctta gaaaaaataa cggtcaatgg acaatcacaa gaaatggtgc
    26461 gcttgattta caaagacaat gatttattgt atgtcaatat aaactcgctc cacaagattt
    26521 caaaatatac aggaaaagaa ggacatgaac ctaaaatcaa taaattaggt agcgatgctt
    26581 ggacaaatct aaaaaataaa accaagaaaa aaattaaaga catcgccgtc gaattgataa
    26641 aactatacgc gcagcgaaaa gtagcaaaag gctttgcttt taacagagat acctatttac
    26701 aagatgagtt agaggcgagc tttatgtatg aagacacgcc tgatcaaagc aaagcaacgg
    26761 ctgaggtaaa agccgatatg gaaaaacctc atccaatgga tcgactgata tgtggcgatg
    26821 tgggttttgg aaaaacagaa attgccattc gtgctgcatt taaggccgtt gctgacagaa
    26881 aacaggtagc tgttttggta cccaccacca tattggcatg gcagcatttt aaaacttttg
    26941 acgctcgttt aaaagaacat ggcgttgcag tagattattt aaatagattt aaatctgcga
    27001 aagaaagaaa agaaacttta gaaaaaacaa aagaaggaaa aatagatatt ttaataggca
    27061 cacatgcttt gctcaataaa gctcttgagt ttaaagactt gggcttatta attatcgatg
    27121 aagaacaaaa atttggtgtc tctgccaaag aaaaattaag acaattttct gccaatgtcg
    27181 ataccttgac attaacagca acgccaattc cacgaacatt aaaattctct ttgatgggcg
    27241 cgcgtgattt atctaatatc atgacaccac cagcaaatcg tattccgata actacagaag
    27301 tacaagtatt tgatgttaaa aacttgaaag aaatcataga gtttgaagtg tttagaggtg
    27361 ggcaagttta cttcgttcac aatcgtgtga aagacttggt agaactggaa acaatactgc
    27421 gacgtttgtg tcctgaagta agcatcagaa cagcacatgg tcagctcgat ggcgaccagc
    27481 tcgaagagat tatgttgcag tttataaacg gcgaatttga tgtattgctc agcaccaata
    27541 ttgtagaaag tggcttagat attccgaatg ccaacaccat catcatcaac aatgcacacc
    27601 attttggatt gagcgattta catcaattgc gcggtcgtgt tggtcgttct aacaagaaag
    27661 cattttgtta tttattatcg cctccaaaat caactttaac agatgaagca cgaaaacgtt
    27721 tgcaaacctt ggaggagttt tcagatttag gttctggttt tcaaatagca ctgcgcgata
    27781 tggatattcg tggcgctgga aatttattag gcggcgagca aagtggtttt attacagata
    27841 ttggatttga aatgtatcat aaaattttag atgaagcgat acaagaactg aaatacacag
    27901 aatttaaaga cgtattcaaa gagcaaatcg aagaacaaaa acaatttgta tatgactgca
    27961 ctattgatac tgatgtggag atgttgattc caccagatta tgtacagaat accgaagaac
    28021 gattgcgttt atacacagaa ttagatgcca ttgaaaatga agaaaaacta aaagcatttg
    28081 caaaaaaaat agaagaccgt tttggaaaac taccaaaaca agtcaatgaa ttatttgatg
    28141 gtttgcgcat tcgatggatt gccaagaaaa ttggatttga acgaattatt ttaaaaaatg
    28201 gaaaattacg ctgttatttt ttagacaatc cgaaatcgcc gtattatgaa agtccctact
    28261 ttccacgcgt aatggaacat atacaaaatt ctaaaaagaa atgcacctta aaacaaagcg
    28321 gcaatagttt gattttggtg tacgacaaca tcaaaaccat gcacgaaaca gaatctattt
    28381 ttaaagaagt agatgagaaa gtgtttggta aatcaatcgt agaaaaaata gaatagacat
    28441 ctatttagtt tccaagcaga gtctcttgaa agggactctg cgagagttgt tggttcgtga
    28501 aattaattac gcagcgtccg gctttgcttc ttatctactc aaagagaccc tgctaggaaa
    28561 gaaggcttag aacaaacggt tttgatgaag gatttgtttt gtaataagtt tataattttt
    28621 ttgtaacttt agtaatcaaa gaagtacaaa atgcaaacca tcaaaataga aataaacaat
    28681 gatgctgtac tgaaacttat agaggagtta gaaactaata agtttataaa aattataaaa
    28741 gataagaaaa aaaccaagtc ttctaaaaaa ttatctgcac ttttattagg aagcattaac
    28801 aataaagaag cagcaaaaat gaaggctgag ttaaaaaata tgcgcaaaga atgggaaaga
    28861 actatctaat agataccaat gttatcatag attttgcgca aaataaatta ccgagacaat
    28921 ctgtttcatt tcttattgat ataattgata atgaaccaaa aatttccatt ataaataaaa
    28981 tagaacttct tggttttgcc aatacaatca aaccaataat tgagtttgta aacgcatcta
    29041 taatctatga cttggatgat gctgtttaaa taaaacgatt gaacttcgta aaaaatataa
    29101 attaaaattg ccagatgcta ttattgcagc tactgctatt gtttataaac ttacaattat
    29161 cacgcataat ttaaatgatt tcaaaaatat taaaagctta aaaataattg attcttattt
    29221 actcatatag taaatcaatt a
//
LOCUS       DAOQQV010000020        29041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_477_length_29041_cov_8.023908, whole genome shotgun sequence.
ACCESSION   DAOQQV010000020 DAOQQV010000000
VERSION     DAOQQV010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 29041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29041
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_477_length_29041_cov_8.023908"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..681
                     /locus_tag="PLL59_03770"
     CDS             217..681
                     /locus_tag="PLL59_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HMY22954.1"
                     /translation="MAIDSKIFSEVKNIFSTYLENNKQRKTPERFSILEEIYNREDHF
                     DAEELYLQMKNNKFNVSRATVYNTLDVLVQCELVTKHQFGQNHAMYEKSYGNRNHDHL
                     ICVDCGKVFEFCDPRLQQVKMSAGELFNQTIKSHSLTLYGGCKIADCEHKKS"
     gene            691..2037
                     /locus_tag="PLL59_03775"
     CDS             691..2037
                     /locus_tag="PLL59_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HMY22955.1"
                     /translation="MILFRRFNIFLMLFAFAVLQVKAQTTILPKPNSIQFKDGTFSYQ
                     KGFDIKIIRGDDATKKIQKQLTDFVKSKNIPVVQFATTAITINLLNAKDTDLSPESYT
                     LSIAPNTISIASTGNAGLYYGIQSIMQLLRADTTKKLTCLEIKDIPQFTYRGFHLDAL
                     HHYFDVETIKQYIDAAAKLKLNQFQWQFADEKMCRIMVKNQNTTSRVEQIYTTTQVQE
                     IVKYAQERYVNIVPVFSFSADSADANLTSKKLFIDELSILFPGTFVAVDFTSLSDSVS
                     AYLTFKNKKIISSEQSSNNSIIKQFYKSTKAAYSRAATGNDVLLSPMKYTSFDFYQDW
                     DDEKKDFHMSLLTLEKAYSFAPLGKIKDSKTIEHIIGIQSMLYTPYISTREELQYMAF
                     PRLLAFAECAWTRKEYKNFADFEKRLKSLKNYFFIEKELPPIDLVRINPNKSKKKK"
     gene            complement(2133..2498)
                     /locus_tag="PLL59_03780"
     CDS             complement(2133..2498)
                     /locus_tag="PLL59_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HMY22956.1"
                     /translation="MRAVAYIYCQKMMLTNFWIWLAIISSILTNIGFKYAAMVLGNPT
                     KKWLTFAGALVFGLLNSVCMTEALKTIPLNIASALFFSLTIIGLLFASYFIFKDALGW
                     KQIIGTAIIIVGVVVVNLK"
     gene            2641..3372
                     /locus_tag="PLL59_03785"
     CDS             2641..3372
                     /locus_tag="PLL59_03785"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HMY22957.1"
                     /translation="MLYNANCYSQSKNKQEQASIWIRYNQQLQLPKNCMLKSEFDERF
                     FIAKALKQHQFLFRVTVDKQLKKNWTIGAGISMWYMGTNNELATSKLMIPEWRPHVEI
                     NHKNSINKRFSISQRWRAEWRFIKKTNPAFTETINGYNNNFRFRYQILLDFAAYQKEE
                     KSLHLIIFDEILLNAGKSILYNIFDQNRIGCSVRYNFNKTIGIELGYINQFQQRSSGI
                     DFYNRQVLRLTLHTNFTIQKNEINK"
     gene            3375..3839
                     /locus_tag="PLL59_03790"
     CDS             3375..3839
                     /locus_tag="PLL59_03790"
                     /inference="COORDINATES: protein motif:HMM:NF021362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HMY22958.1"
                     /translation="MNIKHHISFLKGVSWRVVATIDTILLSFIYTGSIGDALRIGFIE
                     VFTKIFLFYLHERVWIRVKWGTKISQLVNEENKPCLDTDTRNQLKVQEEHYRSLIKGI
                     SWRFFGTMDTIIIATFITGDYSQALKIGATELITKIILYYFHERLWMRFTKK"
     gene            3951..5213
                     /locus_tag="PLL59_03795"
     CDS             3951..5213
                     /locus_tag="PLL59_03795"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HMY22959.1"
                     /translation="MLVDVILGLQWGDEGKGKIVDYLADKYDIIARFQGGPNAGHTLY
                     IDGVKHVLHTIPSGIFRKGKINYIGNGVVIDPITLVKEIENLEKQGVPVQERLVISKR
                     ANLILPSHKILDAASENAKGASKIGSTLKGIGPTYMDKTGRNGLRFGDIIEEDFIEKY
                     ELLKAKHLQLLQMYNFDFDLNEMEKEWFEAVEKLKTLKQVESVYFINEALKKGKKILA
                     EGAQGSMLDIDFGTYPFVTSSNTISAAVCTGLGISPKHIGEVIGITKAYCTRVGSGPF
                     PSELEDEVGEKLRQVGGEFGATTGRPRRCGWIDLPALNYACLINGVTKLIITKADILN
                     SFEEFGVCTHYAYDNKETKDLPYDMVNRKIKPTTIYFKSWGNIDAQQAMPAELLAYVD
                     YLEKYLNTKVAMVSNGPGREQLIELADK"
     gene            5248..6573
                     /locus_tag="PLL59_03800"
     CDS             5248..6573
                     /locus_tag="PLL59_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HMY22960.1"
                     /translation="MRTFFSYGVDDIEQFKLQLLAIAQSTQYFCYLDSNNYPNYPYST
                     FGSLFAIDAIDVLSVSDNCLAKLSTFQQAHKDWIFGFINYDLKNEIEASLYSNNYDKI
                     ENPILHFFVPKYLVKIENDKVEIGIVCTNETECNDELGDFKKLITHHFKSSTNQSNQT
                     PYSSPQISQRFSRRDYVETVNKIKQHIQRGDIYELNFCQEFYAENCKINPIQVFHHLN
                     AISKAPFTSFFKYKENYLMCASPERFLKKQNDTLISQPIKGTRKRLKNEKEDNQIREQ
                     LFFDNKERSENVMIVDLVRNDFSKIAIQNTVEVEELFGIYTFEQVHQMISTISCRIDS
                     SMSFATILEAVFPMGSMTGAPKLSAMQLIEKYEKTKRGLFSGAVGYITPKGNFDFNVV
                     IRSILYNANLKYISVQAGSAITIDCDAEKEYEECLLKAKAMLEALNKNA"
     gene            complement(6642..7157)
                     /locus_tag="PLL59_03805"
     CDS             complement(6642..7157)
                     /locus_tag="PLL59_03805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMY22961.1"
                     /translation="MLLVNKDMVVSVHYELQVDKDGQLVTADKSNADQPLVYLHGAGM
                     LLPDFENNLLGKTVGDTVSFKVSAENGYGVRNEQDIVRVGIENFKDKEGNIDTTMVRV
                     GNILPMMDDQGHQFQGIVCDVTEEAVIMDFNHPMAGKELNFTVTVADVRAATAEELAH
                     GHVHGPGGHHH"
     gene            7420..8070
                     /locus_tag="PLL59_03810"
     CDS             7420..8070
                     /locus_tag="PLL59_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22962.1"
                     /translation="MDSAKKFQNAFDTLLKDFNAKVEEVKSNIQNLTADVKIQVDGQK
                     ENLQEYGEKIKGRITQIVDVDKARANVLAEAENLVDDAKIRIEKVFQFINEQVNLGEK
                     KAKKAANDFSKKAEQFTHEVEKDVKIASAKIESNIKKAEKNIEKQVAKVTKEVKTAAK
                     TATPAKKAAAPAKKAAAPAPAKKAVAPKAPAKKAAAKAPAKPTAVKAAPAKKKATK"
     gene            8295..8888
                     /locus_tag="PLL59_03815"
     CDS             8295..8888
                     /locus_tag="PLL59_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22963.1"
                     /translation="MKKKFIFCLMATLLFVACKPKTTISSDDGNTKVSIDVSDMQNTT
                     DEMNKKIEELKKLKPLTVAEIKAMLPEELAGMKRTDFNSTSAMGFATGEARYNKDDTT
                     SIHLTIWDCAGEAGSGFYALNYWTKMSLESENEDGYTKTVDFMGGKAVEDFKKYNNAY
                     TLTFTSNDRLMVTIAGENIPLTTLQDIAKSMNFKVNS"
     gene            complement(8977..10590)
                     /gene="ftsZ"
                     /locus_tag="PLL59_03820"
     CDS             complement(8977..10590)
                     /gene="ftsZ"
                     /locus_tag="PLL59_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMY22964.1"
                     /translation="MEFEMFSENNSIIKVVGVGGGGCNAVNYMYNQGIPGVNFGVCNT
                     DKQDLLKSPVPTKIQLGTNLLEGLGAGADPEEGRRAALEAEEEIRMYLSNNTKMVFVT
                     AGMGGGTGTGGAPIVSKIAKELGILTVGIVTIPFSNEGKPKRDKAERGIQNMKENVDA
                     LIVVSNNKLYDIFPDLDIDEAFSKADDILTTAAKGITEIITKPGKINVDFNDVKRVMS
                     NSGVAIIGIGKGSGENRAVVAINEAMNSPLLEDADIYGAKGVVLYITYGNGIKMRELD
                     LITESIQSQTQTDCEIIWGVCKDENLEDDIAITLVATGFQTGEIKRERKQVQRQIEEK
                     VVYTLKEEPKAPVNKEVQAKSEVTPPTKEVNNEHIVFPLIDKVEDKIMDDDLFIPTLK
                     DDIKNENEIEFEINLSAPEMDIKIVDKPRFQMTDDIDDGFEKVNKKETQFTLFETNSD
                     SKAVGEIDAQDLERDLIKKRITLDMEKHHISIYELEKEPAYKRYGLDLEKSTPSEESN
                     FSNFMIYSNSEDPMKIEIRPNAMKDITLD"
     gene            complement(10612..12081)
                     /gene="ftsA"
                     /locus_tag="PLL59_03825"
     CDS             complement(10612..12081)
                     /gene="ftsA"
                     /locus_tag="PLL59_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HMY22965.1"
                     /translation="MQVNNVTNSDMENKVANQAENIVCAIDIGTSKIAALVGRRNQFG
                     KIEVLAIGNCPSFGVQRGVVVNIAKTVDSIKKAVMEAERQSGIKFERVHVGIAGQHIK
                     SLQHRGILTRNNNNDWISYEDIQKLKNEMHKLALPPGDRILHVLPQEYIVDNEPGIHS
                     EPIGMMGIRLEGNFHIITGAETAIQNIERCITIAGLKVASIELEPLASADAVLSSEEL
                     EGGVCLVDIGGGTTDIAIFENNIIRHTAVIPFGGNIITEDLKDLRILRDQAETLKIRY
                     GSAIPSDDLRNKHVAIQSEGTIKTVKNEVSLLTVSKIISARVKEILAHVTFQIKFSKF
                     EKKLIGGIVLTGGGSQLKHLVQLTEMETGQTARLGFPREHLASTKISDVENPIYATGI
                     GLLVRGFKLQDEEALFHQDTIASTPVVKEEIKIEAEGVKVPIAENETIDLTTELIATE
                     PVTEGEKEEVGTPGPRKAKSIFSRLNEWIKSDVDDFDSK"
     gene            complement(12093..12857)
                     /locus_tag="PLL59_03830"
     CDS             complement(12093..12857)
                     /locus_tag="PLL59_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22966.1"
                     /translation="MNKTVRNIIIRISMLVGIVVFVCLMVMAKWNTQHRAVQKIKISI
                     DDKVGNFFVNKAEVVQLINEKFTIKGKVLSGRDLEKIEKTVAIIPQVQYANAYTDDKG
                     ELNIKVIQRIPLFRVYNLQGQSFYVDKHGVKFATTSNYAYKVPVVTGNIVEACDSTRA
                     IQSDELKSVFRVANAIDKNELWKAMIGQYNIIENAHIEMTPRFGNGTIIFGNDANIEQ
                     KLKRLDIFYFDVLRKVGWNYYKVINIMYKNQVVCLK"
     gene            complement(12859..14217)
                     /gene="murC"
                     /locus_tag="PLL59_03835"
     CDS             complement(12859..14217)
                     /gene="murC"
                     /locus_tag="PLL59_03835"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HMY22967.1"
                     /translation="MIQLSNIQRIYFLGIGGIGMSALARFFNLHGKIVSGYDKTCTAL
                     TNELEKEGIRITFDDNIETLDKQADLVIFTPAIPKDHLQYNWYVQHDYKVVKRAQVLG
                     LAANSMFTIAIAGSHGKTSTSSITAHILHEANKSVAAFLGGICINFNSNFIDGNAYAV
                     AEADEFDRSFLHLEPNIALITSVDTDHLDIYGNLAAIEESFQQFCNKIRENGILITNS
                     IVSKNISNNNVKQLRYSLTDTSADYHIKNLQIEDGKYLFDIVHPTGVIENVVSYYGGK
                     HNIENAIGASAIALNVGVSAEDIRNALATFRGVKRRFETHVRSEKHVYIDDYAHHPRE
                     LDATIGAIKELYPNKKLTLVFQPHLFSRTQDLCDEFAVSLSKVDELILLDIYPAREKP
                     IAGVTSQIILDKVSIDNKRILSKQAVLDYVKENASIEVLLTVGAGDIDTIVQPLYNIL
                     EK"
     gene            complement(14245..15333)
                     /gene="murG"
                     /locus_tag="PLL59_03840"
     CDS             complement(14245..15333)
                     /gene="murG"
                     /locus_tag="PLL59_03840"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HMY22968.1"
                     /translation="MQRFIIAGGGTGGHIFPAVAIADALKKDNPNCEILFVGAAGRME
                     MEKVPQAGYKIVGLEVVGLQRSMTIKNIWFLFKLIKSLFQAYSVIRTFKPDACIGVGG
                     YASGPVLFIAALMRIKIFIQEQNSYPGITNKILARFAKNIFVAYDNLAQFFSQHKIVF
                     SGNPVRKDITATLPDKKTALAFFDLDANKKTMLIIGGSLGARTINETIELHLDLFTQN
                     NIQLLWQTGKIYYDGILERTNYKALKDVKIHQFIKDMNMAYSAADIIVSRAGALSISE
                     LCIVGKPTILIPSPNVSEDHQTKNAMALVNKNAAILVNDNNAKDVLGVKVLNLLKDES
                     QMHALSENIKALAKTDAANEIVQKIVNA"
     gene            complement(15394..16593)
                     /locus_tag="PLL59_03845"
     CDS             complement(15394..16593)
                     /locus_tag="PLL59_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HMY22969.1"
                     /translation="MIRRIVDNIKGDKYIWYIIATLSSISVLLVYSSTRSLAYRDTQG
                     DTEYYLLKHGFILCFGLFITYIVHRIDHRNFSRIAQMMLYLSVPLLIYTSFFGVKIND
                     ASRWVRIPVVGFTFQSSDFAKLALIMYTSRMLAKKAGVIKDMKEVLLHIMLPIAIICG
                     LIMFDNLSSAAILFMTCIVVLFIGRVSSKFIFTIITCGVIVAAIIFSLSWFFDDDHGR
                     ADVWLKRVEAWQNPDINADNFYQQKQAYIAIAKGGFLRLAPGKSTQCNFLPDAFSDFI
                     YSTLIEEYGLFGGAFVLFLYLFFMYRLILLVKNSPKAFGALMAVGLGVSIVLQAVIHM
                     GVNVHLLPNTGITLPLISWGGSSIWFNAFALGIILSVSRHNQEREAGKPKAPMKEKQK
                     VIDEIHG"
     gene            complement(16609..17946)
                     /gene="murD"
                     /locus_tag="PLL59_03850"
     CDS             complement(16609..17946)
                     /gene="murD"
                     /locus_tag="PLL59_03850"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HMY22970.1"
                     /translation="MKEKIVILGSGESGVGAAILAQKQGMDVFVSDLGKIKQEYKEEL
                     QTLAIPFEEEQHTAEIILSATTVIKSPGIPDKAPIIKKLVEKNIPIISEIEFAFRFTK
                     AKIIAITGTNGKTTTTSLTYHILEKAGLNVGLGGNIGTSFTRQVANGDKDYFVLEISS
                     FQLDNCITFKPHIAILCNITEDHLDRYNYQFENYIDSKFSISKNQDEQDYFIYCLDDE
                     VTMQYFNKANPRVLKIPFSYYKSISEGACVQQQEIIIQLNKNNFTMPISELSIHGIHN
                     SYNSMAAAISAKLVGVRNEKIRESLEDFQNLEHRLEFVATIKGVDFINDSKATNVNST
                     WYALESMTKPTVLIAGGVDKGNDYTLIKDLVNKRVKAIICLGKDNEKLHAAFASEVGY
                     IVDTQSMEQAVQMAYNMADKGDTVLLSPCCASFDLFENYEDRGKQFKRNVLNL"
     gene            complement(18016..19317)
                     /gene="mraY"
                     /locus_tag="PLL59_03855"
     CDS             complement(18016..19317)
                     /gene="mraY"
                     /locus_tag="PLL59_03855"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HMY22971.1"
                     /translation="MLYYLFKYLQENWGLSGSGLFQYISFRASMAIILSLVISMLIGR
                     RVIKWLRKMQIGESVRDLGLEGQIQKKGTPTMGGVIIIAAIVIPTLLFARLDNVYVVL
                     LLVSTIWMGAIGFLDDYIKVFKKDKEGLRGKFKILAQVVLGLVVGCVLYFHQSVKVRD
                     FNTVFEYKIDANDNIDWNNPIKEYAVPKAMYEGKGIVVGDKFLSHETRSLKTNVPFVK
                     DNLLDYAYFLKPFTDDYQKWGWIIFIPFVIFIITAVSNAANLTDGIDGLASGVSAIVL
                     ITLAIFAYVSGNVIIADYLNVLYIPNTGEVVIVCAALIGATIGFLWYNAFPANVFMGD
                     TGSLMLGGVIATIALIVRKELLIPVLCGIFLVENLSVMLQVAYFKYTKKKYGEGRRIF
                     KMSPLHHHYQKSGYHEAKIVLRFWIVSILLAVITFVTLKIR"
     gene            complement(19392..20858)
                     /locus_tag="PLL59_03860"
     CDS             complement(19392..20858)
                     /locus_tag="PLL59_03860"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HMY22972.1"
                     /translation="MKILQDILETVQAIKIIGDTAIAIHTLQLDSRRVENADVFFAIK
                     GTSVDGHNFIDSVIAKGAVAIICEQIPSTLVNNVTYVQVHDARRASAIMACDFYQHPS
                     KKLTLVGVTGTNGKTSVATLLHQIYVEKGYKTGLISTIVNKINHTDIPSTHTTPDPIA
                     INALLQKMVDENCTYAFMEVSSHAADQDRILGLQFAGAVFTNITHDHLDYHKTFQEYI
                     YAKKKFFDHLSPSAFALSNIDDKRGEVMLQNTAAKKYTYAVKNLSDFHFRIIENSFNG
                     LVIQCDGIDVHLQMVGRFNAYNLLAIYATCVLLGMPKDEALSAITLLKGAEGRFDCIS
                     SANKNNIGIIDYAHTPDALKNVLDTINEIRSHNEQVITVIGCGGNRDKAKRPDMALIA
                     SELSNKVILTSDNPRNELAEDIIEEMKKGVPAHNYNKVLSVTDRREAIRIACSLAQAK
                     DIILLAGKGHEKYQEINGVKYPFDDKKILQETFTAFNK"
     gene            complement(20880..22994)
                     /locus_tag="PLL59_03865"
     CDS             complement(20880..22994)
                     /locus_tag="PLL59_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HMY22973.1"
                     /translation="MNIKSNILFRIYLVGGFCTLFGVAVLAKVFMIQTDKKHNWKAMS
                     DNLTRDTFDIIAERGNIYSADDKLLATSVPYFELHIDFASKAMTQEIFNKNVDSLAYY
                     MTKKIGKNSYQAYRQQLIKARKQKRRYFPLIDKANYTELKEIKQWPLFREGKFKGGLI
                     IETHQERTNPYGILAQRTIGLIRENAQDIGLEEAVDSLLKGVNGKVLKQKIAGGEWVP
                     VKGTGQIEPKNGYDIITTIDLGLQDIAETSLLNSLTTSQAEFGCAVLMEVKTGAIKAI
                     ANLGKNKSGSYSENYNYALGLSNEPGSVFKMAGYLSLFDDGFITTSDSINTNGARGVF
                     GGRVLTDDGHNEQYNFLTPGKAFAVSSNVAIASWLTKYYTGKKEKYYAKLQQFGLTMP
                     THIDLKGERKPFINKPENWSAMSLAWMAHGYEVKLTPLQILAFYNAIVNNGNRVKPYL
                     IQKVLNNGKTIREYKPASSSHIICSERAAAMAKEIMLRVVEDEKGTGRGIKSPYYRIG
                     GKTGTAKMSEGNSGYSDKNLSTFVGFFPAEAPLYSCIVVVGGPQGALTTGGAISAPIF
                     RTMADKIITSNIIANQSINKDSTITNQPIPYFVGTPDDVNAINKRFKIKFDFKEDWDY
                     AFVKSNSSNKIGIGKIDVTQHTVPNVYGMLLDDAIYLLENKGIKVTFSGTGKVKQQSQ
                     TAGTPVIKGSVIHLILNNNKQL"
     gene            complement(23010..23393)
                     /locus_tag="PLL59_03870"
     CDS             complement(23010..23393)
                     /locus_tag="PLL59_03870"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HMY22974.1"
                     /translation="MEENELHTTPTPQPNAFQRIVETVLHVFGIDDFISYAQVIHNFL
                     FIAAIVFIGVLEIFNTHLSVRFYRGINKKQEAVKELRWEYMTVKTEMNQKSKQSVLQT
                     VLEPTGLKTLQEPPKKIEVKKGTLE"
     gene            complement(23418..24311)
                     /gene="rsmH"
                     /locus_tag="PLL59_03875"
     CDS             complement(23418..24311)
                     /gene="rsmH"
                     /locus_tag="PLL59_03875"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HMY22975.1"
                     /translation="MYHIPVMLNECIDGLNIHPDGIYVDVTFGGGGHSKKILEQLSDK
                     GKLFAFDVDDDAKQNVPEDKRFTLIAANYRHIKRFLKLHGITSVQGILADFGISSHQI
                     DEPSRGFATRFDAPLDMRMNTKEGISAKEVVNQYSAEQLQQILGFYGEVINAKTLANK
                     IVEARNRQAIETTKDLVDICKTVAKGIEVKYLSQVFQAIRIEVNDELNAIKEFLQQSE
                     SVLAPQGRLVIMSYHSLEDRLVKNFIKNGVFEGEPEKDIFGKYEHHLKVITKKPIEAS
                     TTELKINSRARSAKLRIAEKI"
     gene            complement(24338..24793)
                     /gene="mraZ"
                     /locus_tag="PLL59_03880"
     CDS             complement(24338..24793)
                     /gene="mraZ"
                     /locus_tag="PLL59_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HMY22976.1"
                     /translation="MIGLIGNYEVKVDSKGRLRLPTNLLQQIPEETRSQYILNKGMGK
                     CLRLFPKTQWESVTKEMSSFSYFRKDESNFLRAFYQLATQVEMDSNDRILLSKRLMES
                     VGISDEVVITAFHDVIEIWDSKTYAATIVEPENFADMADEVWSKLNNNK"
     gene            25099..25557
                     /locus_tag="PLL59_03885"
     CDS             25099..25557
                     /locus_tag="PLL59_03885"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMY22977.1"
                     /translation="MNKRLSTLIISILFYVATCAQSNMLFDNLLEQSKQANKPIFLLF
                     SGSDWCSNCIRFEKYILRDSTFQSYLHEYFLYYQADFPQRKKQSSVEKNQNEALAEKY
                     NPTGIFPNILIISPTMQSITIDYKNDSVEDFIAKLSVIKKWANVKNGTQN"
     gene            25541..26467
                     /locus_tag="PLL59_03890"
     CDS             25541..26467
                     /locus_tag="PLL59_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HMY22978.1"
                     /translation="MEHKINLKLMGSAFEIIVVDEDKTFATRNITLAIEEIKRIEYLL
                     TEFSETSVTTLLNKNAGIMPIQVPKEFFQLVQRAQQISELTQGAFDLTVAPLKKLYNF
                     KQQDFEFPSSKIIKNTLQKVGFKHIQLLPNNNIYLTKKGMRISFAAIGKGYAADCVRK
                     LWQTNKVKSAAINASGDLAVFGTHAKGNSWKIGIPNPENKNEIILSIPLHEGAVATSG
                     DYEQYFMKNDIRYAHTIHPKTGMPVKDIKSVSIFHQSAELCDALATAVFVMGTEIGLH
                     FIEQLPNTHAIIIDAKNNVILSNRIQLQNAIS"
     gene            26454..26669
                     /locus_tag="PLL59_03895"
     CDS             26454..26669
                     /locus_tag="PLL59_03895"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HMY22979.1"
                     /translation="MRFPNLFLLGFLLFATCACTVVKPYQRIYVDDAEMQLSKDYVRK
                     FEEHFQDIREGAYPAGNAAQASGCGCN"
     gene            26675..27913
                     /locus_tag="PLL59_03900"
     CDS             26675..27913
                     /locus_tag="PLL59_03900"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HMY22980.1"
                     /translation="MKKRYILFVMMALSQLAKAQHALSDSTFKQKKLSKTSIELAFSY
                     YQQDGNNSAVTGGIGTEKLTVYAVGTNIQHQFKQYNTLTANFGVDIISSASTDKIDYR
                     VSSASVLDQHVHTNIDYQRQIKNTNVKLGGSIGLGFESDYLAVPMQINCAYTEPSKMR
                     SFQIGFAASFDDLRWGRFNEEHQLKLVYPSELRYKDWYDTYKRKTIQLKFGFSQVINK
                     KMLIGLYPEIIYQSGLLATPFHRVYFADNSLRVENLPKQRFKFPIAIKLHSFIGKRFI
                     LKTQYHFYIDNFGIIANAIELESVVKIIPQLSFAPFFRFYHQSAAYYFKPYMQHTIDE
                     IFYSSDYDLSKFNSYKAGAGFTFYPINKKQKKASVEHVSLRYAYYYRSSKLQAHSISF
                     AVMLAAENGKNKKSQNRDGK"
     gene            27944..>29041
                     /locus_tag="PLL59_03905"
     CDS             27944..>29041
                     /locus_tag="PLL59_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22981.1"
                     /translation="MQNHSIISLIQKIETYFPKEIIKHFVVSKKQRSEKLYKLIAIAK
                     NGEQLERSLLFKKLFGKSYSEKNDYLWRNEVRLLKEELEKFLVQKEHEHHAKNNEAYN
                     DWHLVQAYKKLEYYEGIDEKTELLLKQKDSFASYQYVLDASLIQLYSLRYKIIDVEKR
                     RKSYPEYIQKCNMLLDDLVASYSAQINFHKSYYNWLSYNHEAEERETLTLDTYECLLP
                     KNPISNFFNCLGEALTDTDNKNIDHLIANIDAALENIEPIYKNNKLMQEFRVFALMGK
                     GRELSANGNFEEADIVLKEIKNDIDNLYLHHRTIFYVNYITNLVKCKQYNEVIHILDH
                     EFSTDNLLYKNMLLHNRLVCYMYLRDTKNLAN"
BASE COUNT         9500 a         5191 c         4679 g         9671 t
ORIGIN      
        1 gcttatctat ttattcagag tatgtgaatc ttccgaacac ttatggtgag gacacaaacc
       61 atggctaagg aaagagtttt gttggttgaa aaccaacaaa gacgaagtaa atatttatcc
      121 aaaacaaaat tttgcttact gcatctgttt ctattaacac ctaaatttta acaaataatg
      181 agtaattact aattacaatt tattaaattt gcagtaatgg ctattgattc caagattttt
      241 tcagaagtaa aaaatatttt ttctacttat ttagaaaata acaaacagcg taaaacacct
      301 gagcgttttt ctattttgga agaaatatat aaccgcgaag atcattttga tgcagaggaa
      361 ttatatctgc aaatgaaaaa caataagttt aatgtaagcc gtgctactgt gtataatact
      421 ttagatgttt tggtgcaatg tgagctggta accaaacatc aatttggaca aaaccacgcc
      481 atgtacgaaa aatcgtatgg caaccgcaat cacgaccatt taatttgtgt agattgtggt
      541 aaagtatttg aattctgtga tccaagattg caacaagtaa aaatgagtgc tggcgaattg
      601 tttaatcaaa ccataaaaag ccattcactg accttgtacg gtggctgtaa gattgcagac
      661 tgcgaacaca agaaatccta aataaagcct atgatattat ttcgtcgttt caacatcttt
      721 ttgatgctat ttgcatttgc tgttttgcaa gttaaggcac aaacaactat ccttccaaaa
      781 ccaaattcca ttcaatttaa agatggcact ttttcttatc aaaaagggtt tgatatcaaa
      841 ataataagag gcgatgatgc aactaaaaaa atacaaaaac aactaacaga tttcgtaaaa
      901 tcaaaaaata ttcctgtcgt acaatttgcg acaactgcaa ttaccattaa tttattaaat
      961 gcaaaagaca cagatttatc acctgaatct tatacgcttt ccattgcgcc aaatactatt
     1021 tctattgcat caaccggtaa tgctggtttg tattatggca tacaatctat aatgcaactt
     1081 ttacgtgctg atacgacaaa aaaattaact tgcttagaaa taaaagacat tccacaattt
     1141 acatatagag gttttcattt agatgcgcta caccattatt ttgatgtaga aaccatcaaa
     1201 caatatatcg atgcagctgc caagttaaaa ctcaatcaat ttcaatggca atttgcagat
     1261 gaaaaaatgt gtagaataat ggtgaaaaac caaaatacga ctagtcgtgt tgaacaaatt
     1321 tacacaacaa cgcaagtaca agaaatcgta aaatatgcac aagaacgtta tgtaaatatt
     1381 gtgcctgttt tcagtttttc tgccgacagt gctgatgcta atttaactag caaaaaatta
     1441 tttattgatg agctgagcat tttatttcct ggcacatttg tagcagttga cttcacttcg
     1501 ctatctgaca gcgtaagcgc gtatttaact tttaaaaaca agaaaattat ttcatcagag
     1561 caatcatcaa acaatagcat tatcaaacaa ttttataaga gtacaaaagc agcctactca
     1621 agagcagcca caggcaatga tgtgctatta tcgccaatga aatatacatc attcgacttt
     1681 tatcaagatt gggacgacga gaaaaaagac tttcacatga gcttgttaac cttagaaaaa
     1741 gcatatagtt ttgcaccgct tggaaaaata aaagatagca aaaccatcga gcacattatc
     1801 ggcatacaat cgatgctata cacaccttat atatctacac gagaagagct acaatatatg
     1861 gcatttccac gcttattggc ttttgctgaa tgcgcttgga caagaaaaga atacaagaat
     1921 tttgccgact ttgaaaagcg tttgaaatca ctaaaaaact acttttttat agaaaaagaa
     1981 ttgcctccaa ttgatttagt acgcatcaac ccaaataaga gcaaaaagaa gaaataattt
     2041 ttattttaat agacaaaatt tttcaactac aggcaacaca aatctgctgt ctgaatatga
     2101 attttatagt caatagcaag ctatcgtttt acttatttca aattaactac aactacgcct
     2161 acgatgatga tggctgtacc tattatttgt ttccaaccta atgcatcttt aaaaataaag
     2221 tacgaagcaa atagcaatcc gattattgtg agtgaaaaaa ataaagcact ggcaatattc
     2281 aatggaatgg tttttaatgc ttctgtcata cacacagaat taagcaaacc gaatactaaa
     2341 gcaccggcaa acgttaacca cttcttagta ggattaccca aaaccattgc ggcatatttg
     2401 aatccaatat tggttaatat actggaaata attgccaacc atatccaaaa atttgtaagc
     2461 atcatttttt gacaataaat ataagcgaca gctctcaaaa agcataatac taaattaaca
     2521 acacatttag tctatctatt ttatagatta tatagatttg tgtgcaagtc atcaaaatac
     2581 agcattcaag tcaattcatc ttatatgcat ttaaaaagct gctacatctt attgtttata
     2641 atgctatata atgcaaattg ctattcgcaa tcaaaaaaca aacaagaaca agccagcatt
     2701 tggatacgtt acaatcagca attgcaatta ccaaaaaatt gtatgctaaa atcagaattt
     2761 gatgaacgat tctttatagc aaaggcattg aagcagcatc aatttttatt tcgtgtaaca
     2821 gtagataagc agttgaaaaa aaactggacc ataggagctg gaatttctat gtggtatatg
     2881 ggcacaaata atgaattggc tacttcgaaa ttaatgatac cagaatggcg acctcatgta
     2941 gaaatcaatc ataaaaattc tatcaataaa cgatttagca tttcgcaaag atggcgtgcg
     3001 gagtggcgat ttataaagaa aaccaatccg gcttttacag aaacgataaa tggctacaac
     3061 aataatttcc gatttcgata tcagattctt cttgattttg cagcctatca aaaagaagaa
     3121 aaatcattgc atttaatcat attcgatgaa atactcttaa atgcgggaaa atctatactt
     3181 tataacattt ttgaccaaaa ccgaataggc tgttctgtac gctacaactt caataagact
     3241 attggaatag aactaggcta tattaatcaa tttcagcaaa gaagcagtgg tattgatttt
     3301 tacaacagac aagtattgcg cttaacttta cacactaatt ttactataca aaaaaatgag
     3361 ataaataaat aattatgaat ataaaacatc atataagctt tctaaaagga gtttcttggc
     3421 gtgtggttgc taccatagac accatcttac tatcctttat ttacactggc agcataggag
     3481 atgcattaag aatcggcttc atcgaagtat ttactaaaat cttcttattc tatcttcacg
     3541 agcgggtttg gatacgagta aaatggggaa ccaagattag ccaattagta aatgaagaga
     3601 acaagccttg tttagatacc gacaccagaa accagttaaa agtacaagaa gaacattata
     3661 gaagcctaat aaaagggata agctggcgtt tctttggtac tatggatacg attattattg
     3721 ccacatttat tactggtgac tattctcaag cgctcaaaat tggagcaaca gaacttatta
     3781 ccaaaataat tctatactat ttccatgaac gattatggat gcgttttaca aaaaaatgaa
     3841 ataccaatca aaacactaac ctaaatttta tgttcaataa ttgtttttac taaataaatt
     3901 tctcaatttc aaactttaat tagtataaac aaataataga cacacaaact atgctagtag
     3961 atgtaatttt aggcttacaa tggggcgacg aaggaaaagg gaaaatcgtt gattatttag
     4021 cagataaata tgatatcatt gcacgctttc aaggtggtcc aaacgcagga cacacattgt
     4081 atattgatgg tgtaaaacat gtgttacata caattccttc tggtattttt cgcaaaggca
     4141 aaatcaatta catcggtaat ggagtagtta tcgacccaat tacattagta aaagaaatcg
     4201 agaatttaga aaaacaaggt gtgccagtgc aagagcgttt agttatttct aagcgtgcta
     4261 atcttatatt accatctcat aaaatactag atgctgcatc tgaaaatgca aaaggtgcct
     4321 ccaaaattgg ctctacttta aaaggcatag gaccgaccta tatggacaaa actggcagaa
     4381 atggcttgcg ttttggcgat attattgaag aagattttat tgaaaaatac gaactgctaa
     4441 aagctaagca tttacaacta ttacaaatgt ataatttcga tttcgacttg aatgaaatgg
     4501 aaaaagaatg gtttgaggca gttgaaaaat taaaaacgct taagcaagta gagtctgtat
     4561 attttattaa tgaagcttta aaaaaaggca aaaaaatatt agcagaaggc gcacaaggtt
     4621 ctatgttaga catcgacttt ggcacttatc catttgttac ttcttccaac actatttcag
     4681 cggcggtatg cactggctta ggcatttcac caaaacacat aggcgaagtc attggaatta
     4741 caaaagcgta ttgtacgcgt gttggtagtg gcccttttcc atcagaatta gaagatgaag
     4801 taggcgaaaa acttcgacaa gtaggtggcg aatttggtgc aactacaggt cgtccgcgca
     4861 gatgtggctg gattgatttg ccagcattaa attatgcatg tttaatcaac ggcgttacca
     4921 agcttatcat tacaaaagca gatatcttaa attcttttga agaatttggc gtatgcacgc
     4981 attatgcata tgacaataaa gaaaccaaag atttacctta tgatatggta aatagaaaaa
     5041 taaaaccgac aacaatctac tttaaaagct ggggaaatat tgacgcacaa caagcaatgc
     5101 cagctgaact attagcgtat gttgattatt tagaaaaata tttgaacaca aaagtagcca
     5161 tggtttctaa tggtcctgga cgcgaacaat taatcgaatt agcagataaa taggcaatcg
     5221 ccatttttat taaacaaaaa tcaaaacatg agaacatttt tttcttatgg tgtagatgat
     5281 attgaacaat tcaagttaca gcttttagca attgcgcaat ccacacaata tttttgttat
     5341 ctcgactcta ataattatcc gaattatcca tactctactt ttggctcttt gtttgccatt
     5401 gacgccattg acgtgttatc tgtttctgac aattgtttag cgaagctaag tacatttcag
     5461 caagcgcata aggattggat atttggtttt atcaattatg atttaaaaaa tgaaattgag
     5521 gcaagcttat attctaacaa ttatgataaa atagaaaatc caattttaca tttcttcgtg
     5581 ccaaaatatc tggtaaaaat agaaaacgat aaagtagaaa ttggaatagt gtgtacaaac
     5641 gaaacagaat gcaatgatga attaggcgat tttaaaaaac taattacaca ccactttaaa
     5701 agtagcacaa atcaatctaa tcaaacgcca tatagttctc ctcaaatttc acagcgattt
     5761 tcacgaagag attatgtaga aacggtaaat aagataaaac aacacataca acgtggcgat
     5821 atttatgaat tgaatttttg tcaagaattt tatgcagaaa attgtaaaat aaatccaata
     5881 caagtatttc atcatctcaa tgcgatttcg aaagctccgt ttacatcgtt ttttaaatac
     5941 aaggaaaatt atttaatgtg tgcctcacct gagcgatttt taaaaaagca gaacgacaca
     6001 cttatttcac aaccaattaa aggcacacgc aagcgtttaa aaaatgaaaa ggaggacaat
     6061 caaataagag aacaattatt tttcgacaat aaagagcgta gcgaaaatgt aatgatagtt
     6121 gacttagtgc gtaatgattt ctcaaaaatt gctatacaaa acacagtgga agtagaagaa
     6181 ctttttggca tctatacctt tgagcaagta catcaaatga tatctacgat tagctgtaga
     6241 atagatagtt ctatgtcttt tgcaactata ttagaagctg tttttccgat gggctcgatg
     6301 actggtgcgc ctaagctttc tgcaatgcaa ttgattgaaa aatatgaaaa aactaagcgt
     6361 ggattatttt ctggtgcagt aggttatatt acaccaaaag gaaattttga tttcaatgtg
     6421 gtcatacgaa gtattttgta caatgcaaac ctaaaatata tctctgtaca agctggcagt
     6481 gcaataacta tcgattgtga tgccgagaaa gaatacgaag agtgcttact aaaagccaag
     6541 gcgatgttgg aagcattgaa taaaaatgct taataaaaaa tagaaaaagg tttgaaaaaa
     6601 tgcttcaaac ctttaacttt ttatgaaaaa tatttcatcg attaatggtg atgaccacca
     6661 ggaccgtgaa cgtgaccatg tgccaattcc tcagcagtag cagcacgtac atcagcaacc
     6721 gtaacagtaa aatttaattc tttacctgcc attggatgat taaaatccat gataacagcc
     6781 tcttctgtaa catcgcaaac gatgccttga aattgatgtc cttgatcgtc catcatcggt
     6841 aaaatattgc cgacacgcac catagttgta tctatgttgc cttctttatc tttaaaattt
     6901 tctatgccta cacgtacgat gtcttgctca ttgcgaacac catagccatt ttcagccgac
     6961 actttgaagc ttactgtatc gcctactgtt ttacctaata aattattttc aaaatctggc
     7021 aacaacatgc ctgcgccatg tagatatact aatggttgat ctgcatttga tttatctgca
     7081 gtaaccaatt gaccatcttt atcaacttgt aattcgtagt ggacgcttac caccatatct
     7141 ttatttacta ataacataat taaaattttg gcaaagatac ttaaaagaaa tgagatataa
     7201 atgtgagatg tgaaaagtga aatatgcgac gtcgtgtaaa aaaacaggct ggtgcctata
     7261 ttataaataa aaataatcta taagcactta acctgaaatt tgcataaaac atgaactaag
     7321 ttaaaataga atggctttaa attcaacaat acttaatatc tgtctaacgc ttgattaatg
     7381 acatctatat acatttgtat taacaaaaaa aataaaaaaa tggattcagc aaaaaaattc
     7441 caaaacgcat ttgatacatt attaaaagat ttcaatgcaa aggttgaaga agtaaaaagc
     7501 aacattcaaa acttgactgc tgacgtaaaa attcaggtag atggtcaaaa agaaaatctg
     7561 caagaatacg gtgagaaaat caaaggcaga attactcaaa tcgttgatgt tgacaaagcg
     7621 agagctaatg tacttgctga agcagaaaac ctagtagatg atgctaaaat tagaattgag
     7681 aaagtatttc aattcatcaa tgagcaagta aacttaggtg aaaagaaagc aaaaaaagcg
     7741 gcaaacgatt tcagtaaaaa agcggagcaa tttacacatg aagttgaaaa agacgttaaa
     7801 atagcatctg caaaaattga atcaaatatc aaaaaagcag agaaaaatat tgagaaacaa
     7861 gttgcaaaag ttactaaaga agtaaaaact gctgctaaaa ctgctactcc agctaagaag
     7921 gctgccgcac ctgctaagaa agcagcagca cctgcgcctg ctaaaaaagc tgttgcacca
     7981 aaagcacctg ctaaaaaggc tgccgcaaaa gcgcctgcta agccaacagc tgtgaaagca
     8041 gcacctgcta agaaaaaagc aactaaataa ttagaaaata aattgggttt tatttggtta
     8101 gtaaaatcgt ggcgcacctt cggtgcgcct ttttcttttt atgcgtcagc ctttaacagt
     8161 aaaagattta cataaacagt aattccataa tacttcaaat ctaaataggc gattacacta
     8221 agcaacaatt cagaaaaaag aattaacttt aatagaatca taagattatt gttaacctaa
     8281 taaataaaac cattatgaaa aagaagttca ttttctgcct tatggcaaca cttttatttg
     8341 tcgcatgcaa accaaaaaca acaataagca gcgacgacgg aaacactaaa gtaagtattg
     8401 atgtaagcga tatgcagaac actactgatg aaatgaataa aaaaattgag gagttaaaaa
     8461 aactcaaacc gcttacagtt gcagagataa aagctatgct accagaagaa ttagcaggaa
     8521 tgaaacgaac tgattttaat tctacatcag caatgggatt tgcaactggt gaagccagat
     8581 acaataaaga cgatactact tctatacatc ttaccatctg ggactgcgct ggcgaagctg
     8641 gttcaggatt ttatgcattg aactattgga caaaaatgtc cttagaatca gaaaacgaag
     8701 atggttacac caaaacagtt gattttatgg gtggaaaggc agtcgaagat tttaaaaaat
     8761 ataacaatgc ttacactttg actttcacat caaatgatcg gctaatggtt actattgcag
     8821 gcgaaaatat accattgact actttacaag acattgcaaa atcaatgaat tttaaagtga
     8881 atagttaatg caaaaaaata gcagagatat cattgaaata aaaaactacg cattgctgcg
     8941 tagtttttct tattaacttg aaaaaatact aaaaaattag tcgagtgtga tatctttcat
     9001 agcattaggg cgaatctcaa tcttcattgg atcttcacta ttgctataaa tcataaaatt
     9061 cgaaaaatta gactcttctg atggtgttga tttttctaaa tctaagccat agcgtttata
     9121 tgctggttct ttttccaatt cataaatgct gatgtgatgc ttttccatat ctaaagtaat
     9181 acgctttttt atcaaatcgc gctctaaatc ttgtgcatct atttcgccta ctgctttgct
     9241 atctgagttt gtttcaaaca aagtaaattg tgtttccttt ttattcactt tttcgaatcc
     9301 atcatcgata tcatctgtca tctgaaagcg aggcttatct actattttga tatccatttc
     9361 tggcgctgac aaattgattt caaattcgat ttcattttca tttttgatat catctttcaa
     9421 tgttggaata aacaaatcat cgtccattat tttatcttcc actttatcta ttaatggaaa
     9481 aacaatatgc tcattgttta cttctttcgt tggtggcgtc acttccgatt tagcctgaac
     9541 ctctttattt acaggtgctt taggctcttc ttttaatgta taaactactt tctcttctat
     9601 ttgacgttgt acttgtttgc gttcgcgttt gatttcgccc gtttggaaac ctgttgctac
     9661 taaagtaatg gcgatatcat cttccaaatt ttcatcttta caaacgcccc aaataatttc
     9721 acaatctgtt tgtgtttgtg attgtatgct ttctgtaatc aaatccagct cgcgcatttt
     9781 tatgccattg ccataagtga tgtacaagac aacaccttta gcaccataaa tatctgcatc
     9841 ttctaacaaa ggtgagttca tcgcttcatt aatagctaca acagcacgat tttcaccact
     9901 tcctttaccg ataccaatga tggcaacacc actattagac attactcttt ttacgtcgtt
     9961 aaaatccaca ttaattttac ctggtttagt aataatttct gtgatgcctt ttgctgcggt
    10021 tgtcaaaata tcatcggctt ttgagaacgc ttcatcgata tctaaatctg gaaaaatgtc
    10081 gtaaagttta ttattcgaaa caacaataag tgcatcgaca ttttctttca tattttgaat
    10141 accgcgctct gctttatcgc gtttgggttt accttcattg gagaatggaa tggtaacaat
    10201 acctaccgta agaatgccta attcttttgc tattttagaa acgataggtg caccgccagt
    10261 gcctgtgccg ccgcccattc ctgccgtaac aaataccatt ttggtattgt ttgacaagta
    10321 cattctaatt tcttcttctg cttctaatgc agcgcgtctt ccttcttcag gatcagcgcc
    10381 agcacctaat ccttcgagta ggtttgtgcc taattgtatt tttgttggca caggactttt
    10441 taataagtct tgtttatctg tattgcaaac gccaaagtta acgcctggaa tgccttgatt
    10501 atacatatag tttacggcat tgcaaccacc accaccaacg cctacaactt tgatgataga
    10561 attattttcg ctaaacattt caaattccat gatattctaa ttttatggtt attattttga
    10621 gtcaaaatcg tccacgtctg attttatcca ttcgttgagt cgtgagaaaa tggatttagc
    10681 tttgcgaggt cctggagtac cgacttcttc tttctctcct tctgtcacag gttcagttgc
    10741 tattaattct gttgtcaaat caatagtttc attttctgcg ataggaactt taacgccttc
    10801 agcttctatt tttatttctt cttttaccac aggcgttgaa gcaatggtat cttgatgaaa
    10861 aagtgcctct tcgtcttgta gtttaaatcc acgaaccaag aggcctatgc ctgttgcata
    10921 aattggattt tctacatcag atattttagt acttgcaaga tgttcgcgcg gaaatcctaa
    10981 tcgtgcagtt tgaccagttt ccatttccgt gagttgtact aaatgtttta actgcgagcc
    11041 accacctgtt aatacaatac cgccaattaa tttcttttca aatttggaaa atttaatttg
    11101 aaaagtaacg tgtgctaata tttctttgac acgcgctgaa attattttag atacagtcaa
    11161 taaactaact tcgtttttta ctgtttttat ggtgccttca ctttgtatag caacatgttt
    11221 atttctcaaa tcatctgaag gaattgcgct tccatatcga atttttaatg tttctgcttg
    11281 gtcgcgtagt attctgaggt cttttaaatc ttctgtaatg atattgccac cgaatggaat
    11341 tactgctgta tgtctgatga tattattttc aaagatagca atgtctgtag tgccaccgcc
    11401 gatatctact aaacaaacac caccttctaa ttcttcgcta ctcaaaacag catcagcaga
    11461 tgccaatggt tctaactcga tgcttgcaac ttttaaacca gcaattgtga tgcaacgctc
    11521 aatgttttgg attgccgttt ctgcacctgt aatgatatgg aaattgcctt ctaatcgaat
    11581 gcccatcatt ccgatcggtt cactatgaat gccgggttca ttgtcaacta tatattcttg
    11641 tggcaatacg tgtaaaattc tgtcgccagg aggcaatgcc aatttatgca tttcattttt
    11701 tagcttctga atatcttcgt agctaatcca atcattatta ttattgcgtg ttagtatgcc
    11761 gcgatgttgc agacttttaa tatgctgacc tgcaatacct acatgtactc tttcaaattt
    11821 aatgcccgat tggcgctcag cttccataac agctttctta atagaatcca ctgttttagc
    11881 aatgtttact acaacaccac gttgcacacc aaatgatggg caattgccaa ttgctaacac
    11941 ttcaattttt ccaaattgat ttctcctacc tactaatgct gcaatttttg atgtgcctat
    12001 atctatggca cacacaatgt tttctgcttg atttgctact ttgttttcca tatcggaatt
    12061 ggttacatta ttaacttgca tatttcttat ttttatttta aacaaacgac ttgattttta
    12121 tacattatgt ttataacctt ataatagttc cagcctactt tacgcaatac atcaaaataa
    12181 aagatgtcta agcgttttaa tttttgttca atgtttgcat catttccgaa aattattgtg
    12241 ccattgccaa atcttggtgt catttcaatg tgtgcgtttt caataatatt gtattgacct
    12301 atcattgctt tccagagctc atttttatca attgcatttg ccacacgaaa tacacttttt
    12361 aactcatcgc tttgtatggc acgagtagaa tcgcatgctt ctacgatatt gcctgtaacg
    12421 acaggtactt tgtatgcata gttgctggtt gttgcaaact tcacgccgtg tttgtcaaca
    12481 taaaaagatt gcccttgtaa attatacact cgaaataatg gaatccgctg aattactttt
    12541 atattcaact cacctttatc gtcagtataa gcatttgcat attgtacttg tggaattatt
    12601 gcaactgttt tttcaatttt ttctaaatct cttccagaaa gcacctttcc ttttatagta
    12661 aatttttcat ttatcagttg aacgacctct gctttattga caaagaaatt tcctacttta
    12721 tcatctatac taattttaat tttttgtaca gcacggtgtt gtgtattcca tttcgccatt
    12781 accattaagc atacaaatac tactattcct actaacatag aaatgcgtat tataatattt
    12841 cttacggttt tattcatttt atttttctaa aatgttgtat aatggttgaa caatagtatc
    12901 aatatcacca gcaccaactg tcaataatac ttcaattgat gcattctctt ttacatagtc
    12961 taaaactgct tgtttggaca atattcgttt attatcaatg cttactttat ccaatattat
    13021 ttgacttgtc acaccagcta ttggcttttc tcgtgctgga taaatatcta acaatatcaa
    13081 ttcatcaact ttagataatg acacagcaaa ctcatcgcat aaatcttgcg ttctcgaaaa
    13141 gagatgtggc tgaaagacaa gcgttaattt cttatttgga tacaactctt taattgctcc
    13201 aattgtggca tctagttcac gtggatgatg agcataatca tcaatataca cgtgtttctc
    13261 gctacgtaca tgtgtttcaa atctacgctt tacgcctcta aaagtagcca aagcatttct
    13321 aatgtcttct gctgaaacac caacatttaa agcaattgcc gaggctccta ttgcattttc
    13381 tatattgtgt ttcccaccat aataagaaac aacattttca atcacacctg ttggatgcac
    13441 gatgtcgaat aaatattttc catcttcaat ttgtaagttt ttgatatgat aatctgcact
    13501 tgtatctgtc agactatatc gcaattgttt gacattatta ttacttatgt tttttgaaac
    13561 tatgctattc gttatcagta ttccattctc acgaatctta ttacagaatt gttgaaacga
    13621 ttcttcaatt gctgctaaat ttccatagat atctaaatga tctgtatcta ctgaagttat
    13681 taaagcgata ttaggttcga gatgtaaaaa tgagcgatca aactcatcag cttcggcaac
    13741 agcataagca ttaccatcaa taaaattaga attgaaattg atgcagatgc ctcctaaaaa
    13801 tgctgctacg cttttgttgg cttcgtgcaa tatgtgtgct gttatcgacg aagtagatgt
    13861 tttgccatgt gaacctgcaa ttgcaatagt aaacatagaa tttgctgcta agcctaatac
    13921 ttgtgcgcgt tttaccacct tataatcgtg ttgtacatac caattatatt gcaaatgatc
    13981 tttaggaatt gcaggtgtaa aaatcaccaa atcagcttgt ttgtctagtg tttctatgtt
    14041 atcatcaaag gtgatgcgga tgccttcttt ctctaattcg tttgttaatg cagtgcaagt
    14101 tttgtcataa ccagaaacaa tttttccatg taaattaaaa aaacgggcta gtgcgctcat
    14161 gccaatgccg ccaataccga gaaaataaat acgttgtatg ttggataatt gtatcatgaa
    14221 ttttgttctt tttatttatt gattttaagc attaacaatt ttttgtacaa tttcatttgc
    14281 agcatctgtt tttgctaatg ctttgatgtt ttcggacagt gcatgcattt gcgattcatc
    14341 ttttaataaa ttcaacactt taacacctaa cacatctttt gcattattat catttacaag
    14401 tattgcagca tttttattca ccaatgccat tgcatttttt gtctgatggt cttctgacac
    14461 atttggagaa ggtattaaaa ttgttggttt tccgacaata cacaattctg aaatagacaa
    14521 ggcacctgct cttgaaacga taatatcagc tgcactatac gccatgttca tatctttgat
    14581 aaattgatga atttttacat cttttaatgc tttgtaattt gtgcgctcaa gaatgccatc
    14641 ataatatatt tttcctgttt gccacagcaa ttgtatatta ttttgagtaa acaaatcgag
    14701 atgtaattcg atagtttcat ttatagtgcg tgcaccaagg ctgcctccaa tgatgagcat
    14761 cgttttttta tttgcatcta aatcaaaaaa tgctaatgct gtttttttat caggcaatgt
    14821 tgctgtaatg tctttacgca ccggattgcc actgaataca attttatgtt gactaaaaaa
    14881 ttgagctaaa ttatcataag caacgaaaat atttttagca aatcgtgcta atattttgtt
    14941 ggtaattccc ggataagaat tctgttcttg tataaatatt ttgatacgca tcaatgcggc
    15001 aataaacaat acagggccac tagcataacc gcctacacca atacatgcat caggtttgaa
    15061 ggttctgata acgctatagg cttgaaacaa acttttaatc aacttgaata gaaaccaaat
    15121 attttttatc gtcatgcttc tttgcagacc tacgacttct aatccaacaa ttttataacc
    15181 agcttgtggt actttttcca tttccattct gccagctgca cctacaaaca atatctcaca
    15241 atttggatta tcttttttta atgcatcagc aattgctacg gctggaaata tatgcccacc
    15301 agtgcctcca cctgcaatta taaagcgttg cattttttga ttactaattt tgtttgtatt
    15361 agacatgctt ttgtgtgcta attttttgat tggttaacca tgaatttcat ctattacttt
    15421 ttgcttttct ttcattggcg ctttcggttt tcctgcttct cgttcttgat tgtgtctgct
    15481 cacgcttaaa atgattccta atgcaaatgc attaaaccaa atactcgagc caccccaact
    15541 aattagcggc aaggtaatgc ctgtgtttgg tagcaagtga acgtttacgc ccatgtgaat
    15601 gacagcctgc aaaactatac tcacacctaa gcctactgcc atcagcgcgc cgaaagcttt
    15661 tggactattc tttactaaca atattaaacg atacataaaa aagagatata gaaacaagac
    15721 gaatgcgccg ccaaacaatc catattcttc tattagtgtg gaatagataa aatctgaaaa
    15781 tgcgtcaggc aagaaattac attgtgtact ttttcctggt gccaatctta aaaatccacc
    15841 ttttgcaatt gctatatatg cttgtttttg ctggtaaaaa ttatcggcat ttatatccgg
    15901 attttgccat gcttcaaccc gttttaacca tacatcggca cggccatgat catcatcaaa
    15961 aaaccacgat aatgaaaaaa tgatagctgc cacaattaca ccacatgtaa ttatggtgaa
    16021 aataaattta gagcttactc tcccaataaa taacaccact atgcatgtca taaacaaaat
    16081 agccgcagac gataaattat caaacataat taatccgcaa ataattgcaa tcggcaacat
    16141 gatgtgtaat aaaacttctt tcatgtcttt gataacacct gctttttttg ccaacatccg
    16201 agaggtgtac atgatgagtg ctagcttagc aaagtctgat gattggaagg taaatcctac
    16261 tactggaatc cgcacccatc tgctagcatc attaattttt acaccaaaaa aagaagtata
    16321 tatcagtaag ggaacagaca gatataacat catctgtgcg atgcgagaaa aattccgatg
    16381 gtcaatgcga tgaacaatat aagtaataaa caaaccgaag cataaaataa aaccatgctt
    16441 caacaaataa tattctgtat ctccttgtgt gtcacgataa gctaatgagc gagtagaact
    16501 atagactaac aaaacagaga tggagctaag tgtggcaatg atgtaccata tatacttatc
    16561 acctttaata ttatctacta ttcttcttat catgattaat aaatgagatt acagatttaa
    16621 aacattgcgc ttgaactgtt tgccacggtc ttcatagttt tcgaacaaat cgaagcttgc
    16681 gcaacacgga gataacaaaa ctgtatcgcc tttatcagcc atattatagg ccatttgtac
    16741 tgcttgttcc atgctttgcg tatcaacaat atagccaact tctgaagcga atgctgcatg
    16801 taatttctca ttgtctttac ctaaacaaat aattgctttt acacgtttat tcactaagtc
    16861 tttgattaat gtataatcgt ttcctttgtc aacgccaccc gcaatcaata cggttggttt
    16921 tgtcatcgat tctaatgcgt accatgttga gttcacattg gttgctttag aatcattaat
    16981 aaaatctact cctttaatag tagcaacaaa ttctaaacga tgttctaagt tttgaaaatc
    17041 ttccaaactt tctcggattt tctcgtttcg aacgccgact aattttgcag aaatggccgc
    17101 tgccatggag ttgtaggaat tgtgaattcc gtggatggat agttcgctga ttggcatagt
    17161 aaagttgttt ttatttagtt ggataataat ttcttgttgt tgtacgcaag cgccttcgct
    17221 tatcgattta taatatgaaa atggaatttt tagaacacga ggatttgcct tattaaaata
    17281 ttgcatcgtt acttcgtcgt cgaggcagta aataaaataa tcctgttcat cttgattttt
    17341 gcttatgcta aattttgagt cgatataatt ttcaaattga taattatatc tatctaaatg
    17401 gtcttcagtg atattgcaaa gtatggcaat gtgtggctta aacgtgatgc aattatctaa
    17461 ttggaaactg ctgatttcta acacgaaata atctttatca ccattggcca cttggcgagt
    17521 aaatgaagta ccaatgttgc cacctaatcc aacatttaag ccagcttttt ctaagatatg
    17581 gtaggtaagc gatgttgtcg ttgtttttcc atttgtaccg gtaattgcta ttatttttgc
    17641 ttttgtaaat cggaaagcga attcaatttc agaaatgatt ggaatatttt tttcaaccag
    17701 ttttttgatg attggtgctt tatcaggaat acccgggctt ttgatgacag tagttgcaga
    17761 cagaataatt tctgctgtgt gctgctcttc ttcgaatgga attgctaatg tttgtaattc
    17821 ttctttgtat tcttgtttta tttttcctaa atcagataca aaaacatcca taccttgctt
    17881 ttgtgcaaga atggcagcac ctactccact ttcaccagaa cctaatatga ctattttttc
    17941 tttcattttt tctacttatt taaacacatg tgctgtgttt tttttataat agacacatta
    18001 aatgtgttct tttttttatc taatttttaa ggtgacgaat gtaattactg ctaacagaat
    18061 gctgacaatc caaaagcgca atacaatttt cgcttcatga tatcctgatt tttgataatg
    18121 gtgatgaagt ggtgacatct taaaaatgcg tctgccttcg ccatacttct ttttggtata
    18181 tttaaaatat gcgacttgca acatcaccga taaattttca actaagaaaa ttccgcacaa
    18241 cactggaatc agcaattctt ttctaactat taatgcgatg gtagcgataa cgcctccaag
    18301 cattaagcta cctgtgtcgc ccataaatac atttgcagga aaagcattat accatagaaa
    18361 accaatagtt gcaccaatta aagcagcaca cacgataacc acttctcctg tgtttggtat
    18421 atagagtaca tttaagtaat cagcaatgat gacgttgccg gagacataag caaaaatggc
    18481 taaagtaatc agcacgattg ctgacacgcc agaggctaat ccgtcgatac catcagtaag
    18541 gttggctgca ttagaaactg ctgtgataat aaaaattaca aacggaataa agataatcca
    18601 gccccatttt tgataatcgt cagtaaatgg ttttaggaaa tatgcatagt cgagtaaatt
    18661 atcttttacg aatggaacat tggtttttaa tgagcgtgtt tcatgcgaaa gaaatttatc
    18721 gcctacgact attcccttac cttcatacat tgctttggga acagcatatt cttttattgg
    18781 attattccaa tctatattat catttgcgtc aattttatat tcaaaaacgg tattaaaatc
    18841 gcgcaccttt acagattgat ggaaatacaa gacgcaaccc actactaatc ctaatactac
    18901 ttgtgccaag attttgaatt tacctcttaa accttcttta tcttttttga aaactttaat
    18961 atagtcatct aagaaaccga tggcgcccat ccaaattgtt gaaaccaaca agagtacaac
    19021 atatacatta tctaatcttg cgaaaagtaa tgtaggaatt acgatggctg caataatgat
    19081 aacaccaccc attgttggcg ttcctttttt ttgtatttgc ccttctaatc ctaaatcgcg
    19141 aacagattca ccaatttgca tttttcgcaa ccattttatc actcttctac ctattaacat
    19201 agaaatgaca agtgacaata taattgccat tgaagcgcgg aaagaaatat attggaataa
    19261 tcctgagcct gacaaacccc aattttcttg taaatatttg aataaatagt atagcataat
    19321 tacatctaat ttttattaaa ttgaatatct acaaatgcac taattttatt tttcaatttc
    19381 tctttcatca tttatttatt gaatgctgta aatgtttcct gcaaaatttt tttatcatca
    19441 aacggatatt ttacaccatt gatttcttga tatttttcgt gtcctttgcc agcgagcaaa
    19501 atgatatctt ttgcttgtgc aaggctacag gcaatacgta tagcttcgcg tctatctgtt
    19561 actgacaaca ccttattata attatgcgct ggcactcctt tcttcatttc ttcgatgatg
    19621 tcttcagcca attcatttct tggattatca gaagtgagga ttactttatt ggaaagctcg
    19681 ctagcgataa gtgccatgtc aggtcgcttc gctttatcac gatttccgcc acagccgatt
    19741 acggtgatga cttgttcatt atgtgaacga atttcattaa ttgtatctaa tacattttta
    19801 agtgcatctg gtgtatgtgc atagtctatg atgccaatat tatttttatt agcagagctg
    19861 atgcaatcga atctaccttc agcacctttt aataatgtaa tcgcgcttaa ggcttcgtct
    19921 tttggcattc ctaacaaaac acatgttgca taaatagcca ataaattata agcattaaat
    19981 ctaccaacca tttgaagatg aacatcaata ccatcacatt gaattacaag tccattaaat
    20041 gaattttcga taattctaaa atggaaatca ctcaaatttt ttacagcata cgtatatttt
    20101 tttgctgcag tattttgcaa cattacttcg ccgcgtttat cgtcgatatt gcttaatgca
    20161 aaagctgaag gcgaaagatg gtcgaaaaac tttttcttgg cataaatata ttcctggaaa
    20221 gttttatgat aatctaaatg atcgtgtgtg atatttgtaa aaacagcacc agcaaactgt
    20281 aggcctaata ttctatcttg atcagcagca tgtgagctta cttccataaa agcatacgta
    20341 caattttcat ctaccatttt ttgaagcaat gcattgatag caattgggtc tggtgttgta
    20401 tgtgtagatg gaatatcagt atgatttatt ttattaacaa tagtagaaat caaacccgtt
    20461 ttatagcctt tctcaacata aatttgatga agtaaagtag caaccgatgt tttaccatta
    20521 gtgcctgtaa cgcctactaa tgttagcttt ttggaaggat gttgataaaa atcgcatgcc
    20581 attatcgctg atgctcttcg agcatcatgg acttgaacat aagtcacatt atttactaat
    20641 gtggaaggaa tttgttcgca aataattgca actgcacctt tcgctatcac actatcaata
    20701 aaattatgac catcaacact tgtaccttta atagcaaaaa agacatcagc attttctaca
    20761 cgtcgagaat ccaattgcaa tgtatggata gcaatagcag tatcgccaat aatttttatt
    20821 gcttgtaccg tctctaatat gtcttgtaat attttcattt tatttatagt atcgcactac
    20881 tacaattgtt tgttgttatt taaaatcaaa tgaattacac ttcctttaat cactggtgtt
    20941 ccggctgttt gtgattgttg tttcactttt cctgttccgg aaaaagtcac tttaattcct
    21001 ttattttcta acaaataaat tgcgtcatct aacaacatgc catagacatt aggaacagta
    21061 tgttgtgtta catcaatttt tcctatgcca attttatttg aagaattact ttttacaaag
    21121 gcataatccc aatcttcttt aaaatcaaat tttattttga atcgtttgtt gattgcattc
    21181 acatcatctg gcgtacctac aaaatatgga ataggctgat tggtgattgt tgagtcttta
    21241 ttgatagact ggttagcaat aatatttgaa gtaataattt tatctgccat tgttctaaat
    21301 attggcgctg aaattgcgcc gcctgtagtc agtgcacctt gcggaccacc aacgacaaca
    21361 atacaagaat acaatggtgc ttcagcagga aagaagccta caaaagtcga tagattttta
    21421 tcagaatatc ctgaatttcc ttcgctcatt tttgcggtac ctgtttttcc acctattcta
    21481 taataaggtg atttgatgcc tctacctgtt cctttttcgt cttcaactac acgcaacata
    21541 atttctttcg ccatagcagc tgcgcgttca ctacatatta tgtgtgatga agatgctggt
    21601 ttatattctc taatcgtttt accattgttc aatacttttt gaattaagta tggttttaca
    21661 cgattgccat tattgacaat tgcattataa aaggcaagaa tttgcaatgg cgttaattta
    21721 acttcatagc catgtgccat ccaagccaat gacatagcgc tccaattttc tggtttattg
    21781 ataaatggtt ttctttcgcc tttcaagtcg atatgcgtcg gcattgtgag accgaattgt
    21841 tgcaacttcg cataatattt ttcttttttt cctgtgtaat attttgtaag ccaagaagca
    21901 atggccacat tagaagaaac tgcaaatgcc ttgcctggcg ttaaaaaatt atactgttcg
    21961 ttatgtccat catctgttag cactctaccg ccaaaaacac cacgtgcacc atttgtattg
    22021 atggaatctg aggttgtgat aaatccgtca tcaaatagag acaaatagcc agccatctta
    22081 aatacggaac caggctcatt acttaaacct aaagcataat tataattttc agagtagcta
    22141 ccacttttgt tttttccaag attagcgata gcttttatcg ctcctgtttt tacttccatg
    22201 agcacggcgc aaccaaattc tgcttgcgaa gtcgttaatg aatttaataa agaagtttca
    22261 gcaatatctt gcaagccaag atctatcgta gtgattatat cgtaaccatt tttaggttct
    22321 atttgtccag ttccttttac tggcacccat tcgccaccag caattttttg tttaagcact
    22381 tttccattca caccttttaa taatgaatca acagcttctt ctaggccgat gtcttgcgca
    22441 ttttcgcgaa ttaaaccaat tgtacgttgt gctaaaatgc cataaggatt tgttcgttct
    22501 tggtgtgttt cgatgattaa tccaccttta aatttgcctt ctctaaacaa tggccattgc
    22561 tttatttctt tgagttctgt gtagtttgct ttatcaatta atggaaaata tcttcttttt
    22621 tgttttcttg cttttattaa ttgttgacga taggcttgat atgaattttt gccaattttt
    22681 tttgtcatgt aataggctaa tgaatcgaca tttttattga aaatttcttg cgtcattgct
    22741 ttagaagcaa agtcgatatg caattcaaaa taaggaacag aggttgctaa taatttatca
    22801 tctgcagaat agatattgcc acgctcggct ataatatcaa atgtgtcgcg tgtcaaatta
    22861 tcagacattg ctttccaatt atgctttttg tctgtctgta tcataaatac tttagccaac
    22921 acagcgacgc caaatagtgt gcaaaatcca ccgacgaggt aaatacgaaa taatatgttg
    22981 gactttatat tcaatgtgta cgatgatttt tattctagcg ttcctttttt tacttcaatc
    23041 tttttgggtg gctcttgtag tgtttttaat cctgttggtt ctaaaactgt ttgcaatact
    23101 gattgcttac ttttttggtt catttctgtt ttcaccgtca tgtattccca gcggagctct
    23161 ttcacggctt cttgtttttt attgatgcct ctataaaagc ggactgacaa gtgtgtatta
    23221 aaaatctcta aaacacctat aaacacaata gctgcaatga ataagaaatt atgaatgact
    23281 tgtgcatacg atataaaatc atcaatacca aatacatgca atacggtttc cactatgcgc
    23341 tggaatgcat ttggttgtgg cgttggtgtt gtatgtaatt cgttttcttc catgctgtgt
    23401 cagctattga atttcattta tattttttct gcaatacgca attttgcact tcgtgctctg
    23461 ctatttattt tcaattctgt tgtacttgct tcgattggtt ttttggtaat cacctttagg
    23521 tgatgctcat attttccaaa tatgtctttc tctggttctc cttcgaaaac gccatttttt
    23581 ataaaatttt ttaccagtct gtcttctaag gagtgataac tcataatgac taatcttcct
    23641 tgcggtgcta atacggattc actctgttgt agaaattctt taatcgcgtt tagttcgtcg
    23701 ttgacttcaa tgcgaatcgc ttgaaatacc tgtgacaaat atttcacttc aattccttta
    23761 gctactgttt tacaaatgtc cactaagtct tttgtggttt caatcgcttg tctatttcta
    23821 gcttctacaa ttttattggc taatgttttt gcatttatca cttcgccata aaatcctagt
    23881 atttgttgaa gttgttctgc tgaatattga ttgactacct cttttgcgct tatgccttct
    23941 tttgtattca tgcgcatatc gagtggcgca tcaaagcgag tagcaaagcc gcgagatggt
    24001 tcatcaattt ggtgcgatga gataccaaaa tcagctaaaa tgccttgtac gctagttatt
    24061 ccgtgcaatt ttaaaaaacg ttttatgtgt ctataatttg ctgctattaa agtaaagcgt
    24121 ttatcctctg gcacattttg ttttgcatca tcatcaacat cgaatgcaaa taattttcct
    24181 ttatcactga gttgctctaa tatttttttt gaatgtccgc cgccaccaaa agttacatct
    24241 acataaattc catcaggatg tatgtttaat ccatctatgc actcgttcaa cattaccgga
    24301 atatgataca tgtactttgt tttctgtttt taaagaacta tttgttattg tttaattttg
    24361 accaaacttc atcagccata tctgcaaaat tctctggctc gacaatggta gctgcatacg
    24421 ttttgctgtc ccaaatttcg atgacatcat gaaatgctgt tatgaccacc tcgtcgctaa
    24481 tgccaacaga ctccattaat ctttttgaca acaagattct gtcattgctg tccatttcca
    24541 cttgcgtggc taattgataa aatgcacgaa ggaaattgct ttcgtctttt ctgaaataac
    24601 taaagctgct catttcttta gtcacacttt cccattgtgt ttttggaaac aagcgaaggc
    24661 attttcccat gcctttattt aaaatatatt ggctgcgtgt ttcttccgga atttgttgca
    24721 aaagattagt aggcaagcgc aatctgccct tgctatccac ttttacctca taatttccga
    24781 ttaatccaat cataaagaat ttgcaccttt gccatacaaa aatagaacaa aagttacaca
    24841 aattcccact ttacaccact ttttaccata attgaaaatg ttaataagta gaaaaatgtg
    24901 tatatctacc tattgaattg tgtattgaat gtggattatg tttttatttt attgattatc
    24961 attgattaat aatgcacatc agcttataaa catattttaa aaaagtggga aatattagag
    25021 gaagtgtgct gtaaagacga aataagaaat gcagtagtgg atttgctaat tagaaatatt
    25081 ttttaattac attgtaatat gaataagcgc ctaagcactt taattatatc tattcttttc
    25141 tacgttgcaa catgtgcaca atccaatatg ttgttcgata atttattgga acaatcgaag
    25201 caagcaaaca agccgatatt ccttttgttt tctgggtcag attggtgttc gaattgtatt
    25261 agatttgaaa aatacatttt aagagacagc acatttcaat cctatttaca cgagtatttc
    25321 ctatactatc aagctgattt tccacagcgt aaaaagcaat caagcgtaga gaaaaatcaa
    25381 aatgaagcat tagcagaaaa atataatcct acaggaattt ttcccaatat tcttatcatt
    25441 tctcccacta tgcaatctat aactattgac tataaaaatg atagtgttga agattttatt
    25501 gctaagctat cggttataaa gaaatgggca aacgtaaaaa atggaacaca aaattaactt
    25561 gaagctaatg ggctctgctt ttgaaatcat cgtcgtagat gaagacaaaa cttttgccac
    25621 aagaaacata actctagcaa tagaagaaat aaaaagaata gaatatttat tgacagaatt
    25681 ttctgaaaca tcagtcacta cattactcaa taaaaatgct ggaataatgc cgattcaagt
    25741 acctaaagag ttctttcaac ttgtacagcg cgctcaacaa atttctgaac ttacgcaagg
    25801 tgcttttgac cttactgttg cgccattaaa aaaactatat aattttaaac aacaagattt
    25861 tgaatttcca tcttcaaaaa ttataaaaaa cacactacaa aaggtcggct ttaagcacat
    25921 acaattattg ccaaataaca atatttatct tacaaaaaaa ggaatgcgta tcagctttgc
    25981 tgctattgga aaaggatatg ccgcagattg tgtgcgcaaa ttatggcaaa caaacaaggt
    26041 gaaatccgct gctataaatg cgagcggcga cttagctgtt tttggcacac atgccaaggg
    26101 caatagctgg aaaattggca ttcccaatcc agaaaataaa aatgaaatca tcttatcaat
    26161 acctttacat gaaggagctg tagccacttc tggtgattat gagcagtatt ttatgaaaaa
    26221 tgatattcgt tatgcacaca ctatacatcc aaaaacagga atgcctgtaa aagatattaa
    26281 aagcgttagc attttccatc aaagtgcgga actatgcgat gccttagcaa ccgcagtatt
    26341 tgttatggga acagaaatag gcttgcattt tattgaacaa ttgccgaata cacatgcgat
    26401 tattatcgat gcaaaaaata atgtaatttt atctaataga atacaactac aaaatgcgat
    26461 ttcctaattt atttttgctt ggatttttgt tgtttgctac ttgtgcttgc actgtggtaa
    26521 aaccctatca acgtatttat gttgatgatg cagaaatgca attatcaaaa gattatgtaa
    26581 gaaaatttga agaacatttt caagacatta gagaaggtgc ttatccggct gggaatgcag
    26641 cgcaagctag tggttgtggt tgtaattaaa aagtatgaaa aagcgatata ttctgtttgt
    26701 aatgatggca ctttcgcaac ttgcgaaagc acagcacgca ttatccgatt ctactttcaa
    26761 acaaaaaaaa ttatcaaaaa catcgattga acttgcattt tcctattatc aacaagacgg
    26821 caacaactct gcagttacgg gtggcatcgg cacggaaaaa cttactgtat atgccgttgg
    26881 cacaaatatt caacatcaat ttaaacaata caacacacta actgcaaatt ttggcgttga
    26941 tattatttca tctgcatcta ctgataagat tgactatcgt gtttcatctg catcagtatt
    27001 agaccaacat gttcatacga acatcgatta tcaacgacaa ataaaaaaca cgaatgtaaa
    27061 acttggtggt agtattggac ttggttttga atcagattat ttagctgtgc cgatgcagat
    27121 taattgtgct tatacggagc catcaaaaat gcgttccttt caaattggtt tcgcagcttc
    27181 atttgatgac ttgcgttggg gtcgatttaa cgaagaacat caattaaaac tggtttatcc
    27241 atctgaattg agatataaag attggtatga tacttataaa cgaaaaacga ttcaattgaa
    27301 atttggtttt tcacaagtca tcaataaaaa gatgcttatt ggcttgtatc cagaaataat
    27361 atatcaatct ggattattgg caacaccatt tcatcgtgtt tattttgccg acaatagttt
    27421 acgtgtcgaa aatctgccga agcagcgatt caaatttcct attgctataa aacttcactc
    27481 ctttattggc aaacgattta ttttaaaaac acaataccat ttctacatag ataattttgg
    27541 aattattgca aatgccattg agttggaaag tgtagttaaa attattccac aattgagttt
    27601 tgctccattt tttcgttttt atcatcaatc agcagcttac tattttaaac cttacatgca
    27661 acacactatt gatgaaatat tttacagtag cgactacgat ttatctaagt ttaatagcta
    27721 taaagcaggt gctggtttta cattctatcc gataaataaa aaacaaaaaa aagccagcgt
    27781 tgagcatgtt tcattgagat atgcatacta ttatcgctct agcaaattgc aagcacatag
    27841 tatttctttt gcagtaatgc ttgccgctga aaacggcaaa aataagaaat cacaaaacag
    27901 agatggcaag taacattgct caattaatta tatatttgtt cgtatgcaaa accattccat
    27961 aatttcttta attcaaaaaa ttgagaccta ttttccaaaa gagataataa agcattttgt
    28021 cgtttctaaa aaacagcgaa gtgagaaatt gtataaactg atagctattg cgaagaacgg
    28081 agaacagtta gaaagaagtt tgctcttcaa aaaattattt ggaaaatcat attctgaaaa
    28141 aaacgattat ttgtggcgca atgaagtgcg tttgcttaaa gaagagttag aaaaattttt
    28201 ggtgcagaaa gagcatgagc atcatgccaa aaacaatgag gcttataatg attggcattt
    28261 agtacaagct tataagaaat tagaatatta tgaaggaata gatgaaaaaa cagaattgct
    28321 tctaaaacaa aaagatagtt ttgcttctta tcagtatgta ttagatgcta gtttaattca
    28381 attatatagc ttacgttata aaattatcga tgttgaaaaa cgaagaaaaa gctatccaga
    28441 atacatacaa aagtgcaata tgttattgga tgaccttgtc gcttcttata gcgcacaaat
    28501 aaattttcat aaatcttatt acaattggct ctcatacaac catgaagcag aagagcgaga
    28561 aacattaacg cttgacacgt atgaatgttt acttccaaag aatcctatca gtaatttttt
    28621 taattgtctc ggtgaagcac tcacggatac agataataaa aatattgacc atttaattgc
    28681 aaatattgat gcggcgctgg aaaacattga acctatttat aaaaacaaca aattgatgca
    28741 agaatttcgt gtgtttgcat taatgggaaa aggcagagag ctttctgcca atggtaattt
    28801 tgaagaagca gacatcgttc tcaaagaaat taaaaatgat attgacaacc tatatctaca
    28861 tcatagaact attttttatg ttaactatat taccaatctt gtaaaatgca aacagtataa
    28921 tgaagttatt catatcttgg atcatgaatt ttctacagac aatttactat ataaaaacat
    28981 gctcttgcac aatagattag tgtgttatat gtatttgcgt gacacaaaaa atcttgctaa
    29041 t
//
LOCUS       DAOQQV010000021        28647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_490_length_28647_cov_8.825511, whole genome shotgun sequence.
ACCESSION   DAOQQV010000021 DAOQQV010000000
VERSION     DAOQQV010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 28647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28647
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_490_length_28647_cov_8.825511"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3148)
                     /locus_tag="PLL59_03910"
     CDS             complement(<1..3148)
                     /locus_tag="PLL59_03910"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HMY22982.1"
                     /translation="MKRKFVKNTTESFQIIDNKPTVDMLLSKHATLQHLRKFVKYPLL
                     LALVCMTTQFSLQAHDLKQLKEEEIDPSVKEMLHNYDKKIYFSENKGQWPANVIYKAD
                     FKYGQAVATERGMLVGTFDPASLDAVREAGDEEEESIKNGIPYNGLKRGNGNVAGHGW
                     LMNFVGASPDMKIVTSDKHLEKYNYFKASTNNKFSEGANNYQEIWYKNVYKNVDVRYY
                     PSAEGSLEYDIVCKPGFKNDAIAIQFDGIDEMHVTKEGVLSFSTSVGDVTFPQPYVYQ
                     IVKGQKIEIPSSYIVSKKNVLSFNLGKYDETVPLIIDPIALRWATWITNNSDGEDHGH
                     GVWVDQKDGSIYILARIIGSGLITQGPFQSASAGNLDIVLGKYTEPTTVGGSGVRVWQ
                     TYLGGSGDDNPYALEQGPDGNIYLTGYTSSSNFPLLGGSAFSGTSLDARAQSNNNIFI
                     TKINQAGNSIKSAVIGGNANEQSFDIRFDPNGNIIIGGYTNSTNLGTRYGGTASNTNN
                     GGTDALIFKINSDLSTINWMRNYGGSANDQINIMNVKSSNGDIFIGGTTQSTNFPTTS
                     PRQSTIGGSRSGILQRLNSSGTTQWSSYYSSAASQTASILCMEFNRTQDTLYFGGLTT
                     GLDAANISSDAYDNSANGNGDFFIGKMGINQGFKAGTYLGGSQLEDNMMGLNTDENND
                     VYIFGYSRSTNFPTTSDALQLNNLGGQSRGSDKTFTKISSDLKTLKYSTYYGGTRDDY
                     DPVGERGIKFSNCRIYTVVTSTSNDIPLTQGAITTSRPNTNTYEPGLVVWANPPDFIN
                     NTITPNQTICPGVIPANISGSLPSYLLPTIVRNGVTSSYPALGGSNTFQWQSSTDSIT
                     WTNIVGATSQNLPGSLIGPINEKTYLRRIIGGDACVIEQGLVLYVNTLSATATSTNTS
                     CQGSKDGTITVTPKNGTPNYTFLWNDGATSQNRTGLGEGNYSVTITDASGCQVIKSFT
                     ITYTYPKPNAVASASPNVICKGNSTTLSYTSDIAVSVIGWFNNCNAIGASLGTSASFS
                     ISPTTTTTYSIVVQTANGC"
     gene            complement(3615..4883)
                     /gene="glyA"
                     /locus_tag="PLL59_03915"
     CDS             complement(3615..4883)
                     /gene="glyA"
                     /locus_tag="PLL59_03915"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMY22983.1"
                     /translation="MERDKKIFSLINDELHRQTVGIELIASENFTSKQVMEAMGTCLT
                     NKYAEGYPGKRYYGGCEVVDQIEQIAIDRLCELFGATFANVQPHSGAQANAAVFLACL
                     KPGDTILGFDLSHGGHLSHGSSVNFSGKFFRPVFYGVEQETGTIDMDKVEALAHKEKP
                     KLIVCGASAYARDWDYKRFRAIADAVGALLLADIAHPAGLIAKGKLNDPIPHCHIVTS
                     TTHKTLRGPRGGIIMMGNDFENPFGEKTPKGELKMMSAILNSAVFPGMQGGPLEHVIA
                     AKAVAFGEALTDDFGKYADQVMLNARVMAQEFVNRGYNLVSGGTSNHLMLLDLRNKNI
                     SGKDADAALGKADITVNKNMVPFDDKSPFITSGIRIGTAAITTRGLKEQDCLQVVDWM
                     DAIIKDPSNTFLIEEIKAKVNASMQNFPLY"
     gene            5018..6013
                     /locus_tag="PLL59_03920"
     CDS             5018..6013
                     /locus_tag="PLL59_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMY22984.1"
                     /translation="MIAIIPVAGAGTNLRPHTYTQPKSLIPVAGKPILGFIIDQLLEN
                     GVKEFVFVIGYLGEKIKDYVSLNYPNIQKKYVVQEVREGLGHAIWLTKDVIPKKSEVL
                     IVLGDTIIDVDWKKFMQIKTSVLGVKKVTDPRKFGVVAADANGKIMHVVEKPSIPKSN
                     MALVGLYKIKEYDTLIKSIDHNIKHNIRSNNEFYLTDALQEMIHRGVKLESFKVDNWY
                     DCGQKEILLKTNALLLSKRKKVATYKKNLHNCIIIEPIHIGKNSVIKNSIIGPNVTIG
                     DNATIYDSIIRDSIIGSYTKLNDVVLFNSLIGSDSVIWGSSQSLNIGDNTELDLR"
     gene            6057..7361
                     /locus_tag="PLL59_03925"
     CDS             6057..7361
                     /locus_tag="PLL59_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HMY22985.1"
                     /translation="MFAVIDLETTGGNPNAERIIEIGIVLHDGKHKIGEYTTLINPEK
                     EISAFISTFTGITNSMVKNAPKFIDVADTLLELLEGKIIVAHNARFDYNFIKSEFRRI
                     NIPFTKKNICTVQLSRKIFPQFRSHSLGNICKDLGIAVDNRHRAFGDAAATALLLEQL
                     ITHDTKGILDEYLQDDINRIYLPAKLNPKIVEILPEEVGVFYLHNEQGEVIYLDKSKN
                     IREHIFTFFSTKPEEKNRQQLHAEVCDISFELTGNELIASLVEIAEIKKTIPKFNRPI
                     ANAFYKYGLFSETDAQGFHKLMVKLLEEENNPLLKFSSKFKAEKMMHSILSNFRLEPT
                     FKKIDTALQYNKRMQEVLSKYIYPHNNFFIIDNGRGGTERSAIQIENGEYKGFGFFEP
                     AYIQQPEELKCTIQQPLEIIENKKIIQNYIRKYAKQVEIIAY"
     gene            complement(7389..9152)
                     /locus_tag="PLL59_03930"
     CDS             complement(7389..9152)
                     /locus_tag="PLL59_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMY22986.1"
                     /translation="MSTKQKASDWHTLKRVVQLSFPFKRLLFLSVILGILVAVITPLR
                     PYFIQLTVDETIIRQEGKGLEMMGLILLILLIVEFVVKYLFGLYSTTLGQTIMLDLRT
                     RLFAHVQSMRLQYFDKTQVGIITTRTINDIEAINNIFSEGLITIVADVLTLIFVVGFM
                     LYINFKLALVCLITFPIIIIATYIFKEKVRVSFNEVRDKVAQLNSFVQEHISGMRIVQ
                     ISNAQQHEFNKFDKLNQEHYRANNKSVLYYSIFFPVVEVVLALAISLLVWYGAHQVMS
                     DFATIGVFTSFILYLNLAFRPLRMLADKFNTLQMGIIAAERVFKLLDTNEKIENIGKH
                     APTNIQGNIAFENVWFAYNKEDYVLKNITFQVDAGETLAIVGATGSGKSSLINILNRF
                     YEINSGAILLDGINTKQYELSALRSYIGTVLQDVFLFSGTVLDNITLRNPAISYDQVV
                     QASKLVGAHDFIMELPDAYQYKVMERGATLSVGQRQLISFIRALVYQPQILILDEATS
                     SVDTESEHLIQHAIETLVKGRTSIVIAHRLSTIQHADKIIVLEHGEIKEIGAPIDLLK
                     IENGYYRKLYDVSIQQEKLRT"
     gene            complement(9164..9913)
                     /gene="truA"
                     /locus_tag="PLL59_03935"
     CDS             complement(9164..9913)
                     /gene="truA"
                     /locus_tag="PLL59_03935"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HMY22987.1"
                     /translation="MRYFLQLSYNGKNYCGWQRQENAISVQEILETNLSKLFARTIAI
                     TGCGRTDTGVHAKEYFAHFDTDVIPMSDFVYRINSMLPNDIAIQQIIEVRPDLHARFD
                     AISRTYKYFIHFQKNPFIEDQSFCWNKEIDVEKMNNCIRQLIGEHDFSTFEKKGSDNK
                     TSNCILYEAYWQSTDNGLVFTIKANRFLRNMVRRITAALLLLGTNQMQAATLIEAVLQ
                     QKPLDVKLAVPAKGLFLWNIDYGQAIQAINK"
     gene            10012..10458
                     /locus_tag="PLL59_03940"
     CDS             10012..10458
                     /locus_tag="PLL59_03940"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HMY22988.1"
                     /translation="MQSSQNKHQFMIQRIQTIYLLLVSIACIAFIFIPFGIATSAEGI
                     AETVAAKQVIPIMATSIVIAIISLISIFMFSNRKKQMKVVLINIILSLVLIGILIFGI
                     TQHIGIANYSFKIGSILPIFILLFNMLAYGSIKSDEQLVRSMDRLR"
     gene            complement(10459..11292)
                     /locus_tag="PLL59_03945"
     CDS             complement(10459..11292)
                     /locus_tag="PLL59_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22989.1"
                     /translation="MSKKQEIVLGFYWFTCFKPAMLLLFFWNTFSVFSAAPVYVKTID
                     RKPLQLYVNTSNDILLLQKGMLERYTADGIFFQNYGSIYINEHTEIVSVNSFKTILFS
                     PDYGKIIQLDNRLKEIDIIDVNNLGTYLVSCVGSSYDNNFLWLYDAASQRLVKLDKNH
                     TPIFESNTLSLLTQKILQPIQLIESGVLLYLLDEKNGIFVFDNQGNFIKNIPIESLKN
                     IQIIDSKIYYAKGNEVYSYDQLTFLETRYTATPNLQQIHIGKAIVCGTNKDGFVEIWK
                     F"
     gene            complement(11289..12074)
                     /locus_tag="PLL59_03950"
     CDS             complement(11289..12074)
                     /locus_tag="PLL59_03950"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HMY22990.1"
                     /translation="MKSLTLQISTSKKIYFLSDLHLGAPDYENSLARERKVIRFLQSI
                     EHDAQAVFFVGDTFDFWFEYQHVVPKYFVRFFATLIQLKEKGIDIYLFTGNHDLWLKD
                     YLQQEIGVTIFQDKIELNINNPNRDKAATSILVAHGDGLGPNDSKYKILKKIFTNSIC
                     QWLFRWLHPDIGTKIAKWWSRHSFTDPRIEVFKGEEKEWLIQYCKRKLLKKQYNYFVM
                     GHRHLPMEIKLSDESTYINLGDWIVNHQYAVFDGNAMKLNTFE"
     gene            complement(12170..12754)
                     /gene="leuD"
                     /locus_tag="PLL59_03955"
     CDS             complement(12170..12754)
                     /gene="leuD"
                     /locus_tag="PLL59_03955"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HMY22991.1"
                     /translation="MEKFTTIQTSAVPLNVEDVDTDQIIPARFLKATTRDGFGDNLFR
                     DWRFNADHSLKEDFVLNNKNYTGKILVVGKNFGCGSSREHAAWAIHDYGFKVVVSSFF
                     ADIFRNNALNNGLLPVVVSPAFLQKIFTAIENNNAAVFKIDLENQEIEIVATQEKASF
                     DINPYKKTCLLNGYDDIDYLLSMKDKIQAYEIQH"
     gene            complement(12790..14163)
                     /gene="leuC"
                     /locus_tag="PLL59_03960"
     CDS             complement(12790..14163)
                     /gene="leuC"
                     /locus_tag="PLL59_03960"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HMY22992.1"
                     /translation="MATTLFDKIWDAHIVQAIENGPQVIYIDKHFIHEVTTPQAFAGL
                     DSKGIKVFRPQQTVATADHNVPTQNQLEPIKEALSRKQVETLIENCKNHDIELYGLGH
                     PFQGIVHVIGPELGITQPGITIVCGDSHTSTHGAFGAIAFGIGTSEVEQVLATQCILQ
                     TKPKKMRINVDGILGKGVTSKDIILYIISKISTSGGTGYFVEYAGSAIRSLSMEARMT
                     ICNMSIEMGARGGIIAPDEITFAYLKDKKFSAENFEERVDKWKQLKTDEDAVFDREYH
                     FDAADIEPMITYGTNPAMGIKISDTIPNNDDAGLEKALQYMDFQKGQTLIGKTIDYVF
                     LGSCTNSRMEDLRLFCSLIQGKQKAENVTVWIVPGSKQVEKQAIAEGLDKILKDAGMA
                     LREPGCSACLGMNEDKIPAGKYCVSTSNRNFEGRQGQGARTLLASPLMAAAAALTGKI
                     TDVRSLM"
     gene            complement(14425..15078)
                     /locus_tag="PLL59_03965"
     CDS             complement(14425..15078)
                     /locus_tag="PLL59_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22993.1"
                     /translation="MNNKSLIFLTIVFVTLAGCKKEETDKLLANLSTSKAAYEAAANG
                     TWIEITSTEYNSLASSLSNVTRSGTNEADYITASTGAFATNPSAWTIAQDDGNTIPTG
                     SLLFAVKINTATASSTGAKVKISSTSSSAGFSDVGTALPTHAIGEHYFLIKGNTTETT
                     ATGYLGYYKPNGSTIMRNSILGKGAYKFESGDANTLTGTYTDIVFLYQGLSTTTKQW"
     gene            15214..15402
                     /locus_tag="PLL59_03970"
     CDS             15214..15402
                     /locus_tag="PLL59_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22994.1"
                     /translation="MKNIFFYVIAFFMVISAISCGKKSGTCVCKLSGLEISRTPGLSS
                     SDCNSLERGIPDLNCYIE"
     gene            15484..16692
                     /locus_tag="PLL59_03975"
     CDS             15484..16692
                     /locus_tag="PLL59_03975"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMY22995.1"
                     /translation="MEIPISGYVKPGFEKVKTIFEENFAKRKEIGASCCVYHKGEKVV
                     DLWGGFRDKNKKQYWEEDTLCIAFSTTKGISSLAFALLHAKGLIDYEEKVCTYWPEFA
                     SNGKEHITIRQLLSHQAGLFTNDKKLDYAVLTDETALSNCLAKQKPYWNPGDKKGYHA
                     WTIAMYQNEICKRVDPLKKPLHIFFKNEIADALDIDFHIGLPEHIPQHRVAQLIPFFP
                     TEALNGKNSDLGTIMRGVLNPFSLFYKSLLNPPHALNLNNFNKRKFVSIPNGSAHGFG
                     NARSIAQLYNEFATGGKNLHLPRATLNLLEAAPIHPKLSRKDLVLGVDIPFSLGFAKP
                     SIFQNFGVNYRSYGSFGAGGSGGFADPEKQIAFGYLMNKMGTHIANDPRELALRNATY
                     TCVFDIERKG"
     gene            17147..17320
                     /locus_tag="PLL59_03980"
     CDS             17147..17320
                     /locus_tag="PLL59_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22996.1"
                     /translation="MEDKKIHAIYTLFEEEIINIEQYNKDIEDAEEEIDAGNYYTHEQ
                     ALAEIKTWKSKSV"
     gene            17317..17523
                     /locus_tag="PLL59_03985"
     CDS             17317..17523
                     /locus_tag="PLL59_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMY22997.1"
                     /translation="MIEIIWAKRALKGLQKIYEYIAEDSPQNAEKVIIEIAEKVTGLK
                     EQPDRYKLDDFAFVGLQPTNCQAK"
     gene            17697..19613
                     /locus_tag="PLL59_03990"
     CDS             17697..19613
                     /locus_tag="PLL59_03990"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMY22998.1"
                     /translation="MRNLYKFGFFLLSMFLFLGNINANIYPNDRSLLDSTIQPFYHGV
                     ASGDPLNDRVIIWTRVTTDQVSVPVKWRMATDTGMQNIVGQGTFTTDSSRDYTVKVDV
                     VGLQPNTYYYYEFEALGRLSQRGRTKTMPTGSVNNLRIAFMSCSNYSFGYFNAYDAIT
                     RRNDVDLLIHLGDYIYEDGLKTGPYVVDTIKRRTFPEYDAFDLVSYRQRYAWYRLDPS
                     LRNLHQQFPMVVIWDDHEFANDATKDTALRHDPVKQGPWAVRKATAIRVYKEWIPMRE
                     DTSNTNIINFTQPIGDLADIIYTENRIERIDAHSFQQAYDLVSHIDNLPYDTPDRTMH
                     GFRQMEWLTNQLKRSTAKWKVMANQVVFASYVYKQAILGIPFPFHNAAGWDINPLDRK
                     MVLDTVNRYNLQNLVVLSGDIHVSMAFDLPGGAIPYDPATGEGSIGVEFVCDNTVRGG
                     LYGGQQSYMYTNNQHLKYINPNSLGYCVLDLTPTSACCDYWETDTSGKLSSSLKLLGT
                     WCTKINENHLKPYPGPTYTSTVFPALVPYTPRNNGITVGVKNTQRAVELMSVFPNPTQ
                     HYLRFQYFIKAPENLVVTIYDMLGNAVKSVDYGQQGVGLHQDGFPIMDLAPAEYIITF
                     QSGTDIVSRKFIKH"
     gene            complement(19640..19987)
                     /locus_tag="PLL59_03995"
     CDS             complement(19640..19987)
                     /locus_tag="PLL59_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY22999.1"
                     /translation="MLNSSNKSNEKNYIYIEIVQYFIFPPIVIIIGMMAWNGFSSIGE
                     KFGDVSYGTYIYGFPIQQLLLHYFQLDVIGLLLVSLPLTLLFGYLSWHLVEKHALAKK
                     KSIYLQLKKHIPF"
     gene            20005..22533
                     /locus_tag="PLL59_04000"
     CDS             20005..22533
                     /locus_tag="PLL59_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23000.1"
                     /translation="MYKIKLKICICVCVYLIHNAKSQSIPADLGLGSKVVWNKDLMGF
                     NLGDFSNTNVPDNNIFDIKNNGSGNTYNNYTSLIEDAGLGKNILYRFYTGEFGNCYNR
                     WYGGAGGTYKDSLGNMIFDPYSSTFHHIQYNQFNSNLNDSLRDNLYNNYFTYNDTPFI
                     TYQTTEVAKPKNNIIFPFIKLVTNNRAKNAKTVFNLPITAHYRDYRTNNSWKSDLNNN
                     NFALTINFYAKLIGVFSQYADNYSPRKETFSKSSVIAEIYNMNSLNTSVSNIIPTPSN
                     KISPQFKEIVLQNLAAFSTLIQNRVKVVHIELGNELYSRMYNGGYCSWWQANPSFTEF
                     SDKTNNNIFNDVLFYNDPCNNTSPSSTLAKSVNVYGNLIKMYMALIRELVENLWSNES
                     DEIKKAFYLDIKNNLKFGVPMSGRFPNLSSNGFASLDKYLYDNKSTLGFDAFIQHPYY
                     KPNIYNGTLDVKYDSLKKYYSERFNTYYKQTFPKLIRTNYPNISELWFTEWAHEDPNN
                     INNTLLEGMNYIDEFMNFWDLNSNLNLTDTLFRNNDILKVANHHMLWDKYASNKQLIN
                     IQYNQLLPKYRTMFYAHKLLLPIMDNNTRMINKLNGGFVSPNSDIYFRSFESPQSGQD
                     VPGKPGYEFANKIYIYYNNKNNNTTPYTFNLNSAVQSLLANCFFSTQIYSVKRNSIYA
                     NKLYISRGKGPSESSDTITDDPNKTVQYESDLLINDINSWNTLPIAKYSAGYYEITII
                     RETSIGTSASSKSYKNKGYFINSLFPNPLNRGEKINIKVNGEHDLDAKIYISSIDGKL
                     LKTIEYPIQKGENYIKINSDFLSNGIYILHSDDLSIIEKIIVKE"
     gene            22783..24048
                     /locus_tag="PLL59_04005"
     CDS             22783..24048
                     /locus_tag="PLL59_04005"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMY23001.1"
                     /translation="MLDYYTGIKIQQNSSTNRRKYWFWLSVCTNLGFLGIFKYYNFFI
                     ENFIQLFSELGIQIHATTINIILPVGISFYTFHGLSYVIDIYKNRIKAENNFIDYAVF
                     VSFFPLLVAGPIERATHLLPQIKIKRIFEYKYANEGLRQILWGLFKKVVIADQCAQYA
                     DIVFSNSNIYSGSTLLLGAFFFTIQIYGDFSGYSDIAIGTAKLFGIDLLKNFSYPYFS
                     RDIAEFWRRWHISLSSWFKDYLYIPLGGSKGGTYVKVRNTFIIFLVSGFWHGANWTFI
                     IWGLLNALYIMPSIIFNTNRNNLEIVAKGRLFPTIKEFISICITFMLALFAWIFFRSD
                     NLHVACDFIKNIFDYSLFAIPQVKDMRLLLTIAIFFSIEWIGREGSFALEYLEKRLPL
                     LLRWIFYGILTASIVSHVGTDKQFIYFQF"
     gene            24263..24973
                     /locus_tag="PLL59_04010"
     CDS             24263..24973
                     /locus_tag="PLL59_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23002.1"
                     /translation="MIKDSLHYFPINTSIQASLGIVYMMDDVWKYLKKNDVVILVPEY
                     ANFYGNFAYGSEELLRIELDIYPIKNISLRKKQWLNIAKFIPKYAFSKFRPSEYINIK
                     ENDIYGIHSYNQYGDVDAHWYKKSVKYEPITKFHGALNDAVFNSIETFNNKLKSRGIL
                     FYVSYPCFQKTSYNHCAKQINEIEKKLKQSQINILGSPDKYCFDDSLTFNKPYHLTKS
                     GLDRRTQFLIEDLKVAMK"
     gene            24988..26652
                     /locus_tag="PLL59_04015"
     CDS             24988..26652
                     /locus_tag="PLL59_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23003.1"
                     /translation="MHLLNIKKIKLINERNYLKLDAFTITGICLLIFCGIRATMYLEK
                     HMDILFWDESLYLTRGLSMFKYIPRDWGPSYSLWYKFLSYIFSDRVELYYFNFKLTTI
                     LVSVSLFLLLLSCGIQRILAFLFSLLFLSSYINIATWPRVSHFCIIVICAGIILAKRQ
                     HTNLAKFVFLSVAFLICSFARPELFLPFLLCFFITAFLFFYKIKSRTKYEYVLMMSLI
                     VFLVFLYIFFKTPFNNGDTNRGIGVFLQHFAMNYSQWKHDGSIFWLDFPTILKQNFGN
                     AQSLGEMYKANPALIQQHIVSNATNYFTQIGKIILSFFVPLFTKSINWLAIMMAFMLF
                     VVYFSYTKNISNKRQRIFLLLKDNTLTIFVLLLFAMPSVFVCFYAYPREHYILLQTPL
                     LLLIIAIIFSSIAVAIFKTTQKIVVIAVVWFFVMPTAADFSYFRMFRAADNLSNKQTV
                     SYIKKHFTGKDSIHVFDLEGGLTNLLAPNFTNSNYLYLQDREKIKLSAFLQQHQYDII
                     YKTPILTQLEATKSDTSLFDMLQHPEKYGYFEQKTGNFANSLLIKH"
     gene            26654..27973
                     /locus_tag="PLL59_04020"
     CDS             26654..27973
                     /locus_tag="PLL59_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23004.1"
                     /translation="MNFAYKTLYTRDQPLFELKVAGIFIFSLFFASNILIQFNSASKV
                     AGIVIMLFLPFILISAGIYYYYKIFQLKELNPIDIIILLSLLLPFYNAITVNMIFDVK
                     IMKALFNLSSRFYVVMCSLIYYAIRSNKITLKQYLYANLLLSWFCFALYSFVSLTIPP
                     ETFISGVGDGLVGYNPSKGGYIYRFSSALIVFGMIYYFLDYIQKNNILSLFFWMMFLA
                     YQVFIDKGRIELVSVAIPMVIYMVYLLKWHDLIKKVLTILLLIGVVLIIAYLIDPKII
                     QFTADMYWMFIKFFMGEKTGEGSADMRWTEMAAVYNYFLKYPDHIFFGIGTPKSEILQ
                     KNVGTVIFGDIGIVGGLLSQGIVGLIIIYSVFLYPLFIWYKVKHYKHDIFFNVGVLST
                     ITVFIQSLFSGAIFYSFLGIMLPLIIMDYYRVKEKNYWKEQRSKPPA"
     gene            27970..>28647
                     /locus_tag="PLL59_04025"
     CDS             27970..>28647
                     /locus_tag="PLL59_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23005.1"
                     /translation="MKKSIKIIGIVFLIVFVVKVCTSPAKKNTATILQTAKEVVAVTV
                     DADYTTSTLVNPKIFGVNIGFALARELDKDSGFVQLLREMRPHSLRFPGGTVANYYHP
                     HLPVYGYKSNEIPHGLGVLYKVQFERKENILYNFIRLAKQVNCGAVFCANVLTGTTAE
                     TLFVLDELSKNNIPIYGVELGNEFCLLPYRKDVFVTEQVYIDKIKNTAAAIRQKYPNL
                     KIAVVAGV"
BASE COUNT         9403 a         4816 c         4769 g         9659 t
ORIGIN      
        1 tacagccatt agctgtttga acgacaatac tataagtagt tgtagtggtt ggactgatac
       61 tgaatgaagc tgatgtacct aagctagcgc ctattgcatt gcaattatta aaccagccaa
      121 taacagaaac tgctatatca cttgtgtaac tcaatgtcgt gctattgcct ttacaaatta
      181 catttggaga tgcagatgct acagcatttg gtttaggata agtatatgta attgtaaaag
      241 attttattac ctgacagcct gatgcatctg taattgttac agaataattt ccttccccta
      301 atcctgttct attttgtgat gttgcaccat cattccaaag gaatgtataa tttggcgttc
      361 cattttttgg tgtaacagta attgtaccat cttttgaacc ttggcaacta gtatttgtag
      421 aagttgctgt tgcgcttaat gtgtttacat atagaacaag tccttgttct ataacgcaag
      481 catcgccacc tatgatacgt cttaaatatg ttttttcatt gataggaccg attaaagaac
      541 caggaagatt ttgtgatgtt gcacctacaa tatttgtcca agtaatacta tctgtactgc
      601 tttgccattg gaaagtattt gaaccaccta atgctggata agacgatgtt actccatttc
      661 taacaatagt tggcaataaa taagaaggta aagaacctga gatatttgca ggaataacac
      721 ctgggcaaat tgtttggttt ggtgtgattg tattatttat aaaatcagga ggatttgccc
      781 aaacgaccaa accaggttca tatgtatttg tattaggtct agaagttgtt atggctcctt
      841 gcgttaatgg aatatcattt gatgtagatg taactacagt atatattcta caatttgaga
      901 atttgatacc acgttcacct actggatcat aatcatctct tgtaccacca taataagtac
      961 tatattttaa tgtttttagg tctgaactga tttttgtaaa tgttttatca gagcctcttg
     1021 actgaccacc gaggttattc agttggagcg catctgatgt agtagggaaa tttgtactgc
     1081 gagaatatcc gaatatataa acgtcattgt tttcatcagt attcaaaccc atcatattat
     1141 cttccagctg actaccacct aaataggtac ctgctttaaa cccttgattg attcccattt
     1201 ttccaatgaa gaaatcgcca tttccatttg ctgaattatc ataagcatct gatgagatat
     1261 ttgctgcatc taagcctgtt gttaaaccac caaagtataa tgtatcttgt gttctattaa
     1321 attccataca taaaatagaa gcggtttggc tggcagcaga gctatagtaa gagctccatt
     1381 gtgtagtacc actgctattt aaacgttgta agataccgct tctcgaacca ccaattgtcg
     1441 attgtcgagg gcttgtggtt ggaaaatttg tggactgcgt tgttccacca ataaatatat
     1501 caccatttga tgatttaaca ttcatgatat taatttggtc attcgcagaa ccaccatagt
     1561 ttctcatcca attgatggta ctcaagtctg aatttatttt gaaaataagt gcatcagtac
     1621 caccattatt tgtattagat gcagtaccgc catatcttgt gcctaaattg gtactattgg
     1681 tataaccgcc aataataatg ttgccattag ggtcaaatct tatatcgaaa gattgttcat
     1741 tagcattacc gccaataaca gcactcttga tggagtttcc agcttggtta atttttgtaa
     1801 taaaaatatt attatttgat tgcgctcttg catctaagct tgtaccacta aatgcgctac
     1861 cgcctaaaag tggaaagtta gaacttgaag tatatcctgt taaatatata tttccgtctg
     1921 ggccttgttc taatgcataa ggattatcat ctccgcttcc acctaaataa gtttgccaaa
     1981 cacgtacgcc actgcctcct acagttgttg gctctgtata tttccctaat acgatatcta
     2041 agttgccggc gcttgcactt tggaatggac cttgtgtaat taaaccggaa ccgatgatac
     2101 gtgccaagat ataaatagaa ccatcttttt ggtcaaccca aacaccatgt ccatggtctt
     2161 caccatcaga attattagta atccaagttg cccatcgcaa tgcgattggg tcgataatca
     2221 aaggaacagt ttcatcgtat ttacctaaat taaaacttaa tacatttttc ttagaaacaa
     2281 tataagatga aggaatctca attttttgac cttttacaat ttgatataca taaggttgtg
     2341 gaaatgtgac atcacccacg ctcgttgaaa agcttaatac tccttctttt gttacatgca
     2401 tttcatcaat gccatcaaat tgaattgcaa ttgcatcatt tttaaaacca ggcttacaaa
     2461 cgatatcata ttctagagaa ccttcagcag atggataata tcgtacgtct acatttttat
     2521 acacattctt ataccaaatt tcttgatagt tatttgcgcc ttcgctaaat ttattatttg
     2581 tactagcttt gaagtaatta tatttttcca aatgtttatc gcttgtaacg attttcatat
     2641 ctggagatgc gccaacaaag ttcattaacc aaccgtgacc agctacattt ccattacctc
     2701 tttttagtcc attataagga ataccatttt ttattgattc ttcttcctca tcgcctgctt
     2761 cgcgtactgc gtctaatgat gctggatcaa atgtgcccac caacatacct ctttctgttg
     2821 ctacagcttg gccgtattta aagtctgctt tataaatgac atttgctggc cattgacctt
     2881 tattttcgga aaaataaatt ttcttgtcat agttatgtag catttctttt actgaaggat
     2941 ctatctcctc ctcttttaac tgcttcaagt catgcgcttg taatgagaat tgtgttgtca
     3001 tgcacactag tgccaaaagc aatggatact ttacaaattt tctcaaatgc tgtaaagtgg
     3061 cgtgtttaga taaaagcata tctacggttg gtttattgtc aattatttgg aatgattccg
     3121 tcgtgttttt cacaaatttt cttttcatgt ttgtcatacc attcatatca aattcaaaaa
     3181 aaatgaatca gcaagctaaa gggcaataat tatatattgt caattagcct taaaagttta
     3241 aggattcagc aacgaaccaa gaccagtggg gagagtctaa ttctgtttat gtttaactat
     3301 atcaataaaa aattactaca caaaaaactc aaacaagtat ttaaactgct ttatataagc
     3361 gtaataaatt gttatgtcaa tacttactat agggtttctg ttctatactt taaaggtgga
     3421 gaacctagtg ccgaaaaaaa tttacaaagt gaaataaatt gattcaaatc ataaataatt
     3481 caaattcggg attgtaaaga ttagtaaaaa aagttaatta atcaacattt gtgtgaaaaa
     3541 aagttgatat ttatcaaatt tttttcattt tggtaacctt aagttaacaa atttgttaaa
     3601 atttaggatt ggaactagta taaaggaaaa ttttgcatag atgcattcac ttttgcctta
     3661 atttcttcta tcaaaaatgt attgcttggg tctttgataa tagcatccat ccaatctacc
     3721 acttgtaggc aatcttgttc ttttaagcca cgagtagtga ttgctgctgt gccaatacga
     3781 attcctgatg tgataaacgg tgatttgtcg tcaaatggca ccatattttt attaacagtg
     3841 atatctgcct tacctaatgc agcatctgca tctttacctg aaatattttt attgcgtaaa
     3901 tccaataaca ttaagtgatt gcttgtgcca ccagaaacca aattataacc acgattgaca
     3961 aattcttgcg ccatgacacg tgcattgagc atcacttgat ctgcgtactt tccgaaatca
     4021 tctgttaagg cttcaccgaa agcaactgct tttgctgcaa taacatgttc taatggacca
     4081 ccttgcatac ctggaaacac agcagaatta aggatagctg acatcatttt caattctcct
     4141 tttggtgttt tctcaccgaa tggattttca aaatcattgc ccatcataat aataccacca
     4201 cgtggtccac gtagtgtttt gtgtgttgta gatgtcacta tatgacaatg tggaattgga
     4261 tcatttagtt taccttttgc tattaagcct gcaggatgag caatatcggc caacaacaat
     4321 gcacccacag catcagcaat ggctctgaaa cgtttgtaat cccaatctct tgcataagct
     4381 gaagcaccac atactatcaa ttttggcttt tctttatgtg ccaatgcttc caccttatcc
     4441 atatcaattg tgcctgtttc ttgctctacg ccatagaaaa ctggacggaa aaatttacca
     4501 gaaaaattga ctgatgaacc atgtgacaaa tggccaccat gcgacaaatc aaaacctaaa
     4561 atagtatcac ctggctttag acacgctaag aacacagcag cattagcctg tgcgccagag
     4621 tgtggttgta cattggcaaa tgtagcacca aacaactcac aaagcctatc gattgcgatt
     4681 tgctcaattt gatcgaccac ttcgcagcca ccataatagc gtttaccagg gtaaccttcg
     4741 gcatacttat tagtaaggca agtgcccatc gcttccatta cttgtttaga agtaaaattc
     4801 tcagatgcaa ttaactcaat tcctactgtt tgtctgtgta attcgtcgtt gattaacgaa
     4861 aaaattttct tatcgcgttc catgttgcta aattaggtat aagttgcaag taataagcta
     4921 tttgcaattt gtcattacaa aaaaataata ttcaaaacat gtcattttct tttcgccaat
     4981 ttattaacat ttcgctaatt acttctacct ttaaactatg atagcgataa ttcctgttgc
     5041 cggcgcaggc actaatttaa gaccacatac ctatacacag ccaaagtcac tgattcctgt
     5101 ggcaggcaaa cctatattag gatttatcat tgatcaattg ctagaaaatg gtgtcaaaga
     5161 atttgttttt gttattggct atctaggcga aaaaattaaa gattacgttt cacttaatta
     5221 tcccaacatt caaaaaaaat atgtggtgca agaagtacga gaaggcttag gacatgccat
     5281 ttggcttacg aaagatgtta ttcccaaaaa aagcgaagta ctaattgttc taggtgatac
     5341 gattattgat gttgactgga aaaaattcat gcagataaaa acatctgtat taggcgtaaa
     5401 aaaagttaca gaccctagaa agtttggtgt tgtagctgct gatgctaatg gaaaaattat
     5461 gcacgtagta gaaaaaccaa gcataccaaa atcaaatatg gcattggttg gattgtataa
     5521 aataaaagaa tatgacacat taataaagtc gatagaccat aatataaagc ataatataag
     5581 aagtaataat gaattttatt tgaccgatgc cttacaggaa atgatacatc gaggtgtgaa
     5641 acttgaaagc tttaaagtag ataattggta tgattgtgga cagaaagaaa ttctcttaaa
     5701 aacaaatgca ttattgctta gcaaaagaaa aaaagttgca acttacaaaa agaatttgca
     5761 taattgtatc attattgaac ctattcacat tggaaaaaac agtgtaatta agaactcaat
     5821 tatcggacca aatgttacca ttggcgacaa tgcaacaatt tatgattcga ttatcagaga
     5881 ctcaataatt ggcagctata ccaagcttaa cgatgttgtt ttatttaact ctttaattgg
     5941 tagcgactct gtaatttggg gcagctcaca gagcttaaac attggcgata acaccgaatt
     6001 agacttgaga tagttttaat taaataatat agaagcaata tcgctcgtta aaaaatatgt
     6061 ttgcagtaat tgatttagaa acaacaggcg gcaatccgaa tgctgagcgt atcatagaga
     6121 taggcattgt attgcacgat ggcaaacata aaattggcga atacacaact ttgataaatc
     6181 ctgaaaaaga gataagtgcc tttatttcta catttactgg tattactaat tcgatggtga
     6241 aaaatgcacc aaaattcata gatgttgccg acacgctttt agaactttta gaaggtaaaa
     6301 taatagttgc acacaatgct cgattcgatt acaattttat aaaaagcgaa tttcgtcgta
     6361 ttaatattcc atttaccaaa aaaaatattt gcacagttca attgagtcgt aaaatatttc
     6421 ctcagttcag atcgcacagc cttggaaaca tttgcaaaga tttaggtata gcagtggaca
     6481 ataggcatcg cgccttcggt gatgctgctg ctactgcgct tttgctggag caacttatta
     6541 cacatgatac aaaaggaatt ttagatgaat atctgcaaga tgatatcaat agaatctatt
     6601 tgccagcaaa attaaatcca aagattgttg aaatactgcc tgaagaagtt ggcgttttct
     6661 atttacacaa tgaacaaggc gaagtgattt atctagataa atcaaaaaat attcgcgaac
     6721 atatttttac ctttttttct actaaaccag aagaaaaaaa cagacaacaa ttacatgcag
     6781 aagtgtgcga tataagcttt gaattaactg gcaatgagct tatcgcctca ttagtagaaa
     6841 ttgcagaaat aaaaaaaaca attccaaaat ttaatcgacc tatcgctaat gcattttaca
     6901 aatatggttt attttctgaa acagatgcgc aaggttttca caaattgatg gtgaaattat
     6961 tggaagagga aaacaatcct ttgcttaaat tttcttctaa gtttaaagca gaaaaaatga
     7021 tgcattctat tctatcaaat tttagactcg agcctacatt taaaaaaatc gatactgcat
     7081 tacagtataa taagcgcatg caagaagtat tatcgaaata tatttatccg cacaacaact
     7141 tttttattat agataatgga agaggtggaa cagagcgctc tgcaatacaa attgaaaatg
     7201 gtgagtataa aggatttgga ttttttgagc ctgcatatat tcaacaacca gaagaactaa
     7261 aatgcacgat acaacagcct cttgaaatta ttgaaaataa aaaaataata caaaattaca
     7321 ttcgcaaata cgccaaacaa gtagaaatca ttgcttatta gccataatta ttgtttttac
     7381 aaattatatt aagtacgtaa tttttcttgc tgtatagaca catcatataa tttgcgataa
     7441 taaccatttt ctatttttaa taaatcgatt ggtgcaccaa tttctttaat ctcaccatgt
     7501 tctaatacga tgattttatc tgcgtgttgt atggttgaca atcgatgtgc aattactatt
     7561 gatgttctac ctttcaccaa ggtttcaatt gcatgttgta tcaagtgttc tgattctgta
     7621 tcgacactcg aagtggcctc atctaaaatg agaatttgtg gctgatatac taatgcacga
     7681 atgaaggaaa tcaattgacg ttgaccaacc gataaagttg ctccacgctc cattaccttg
     7741 tattgatacg catctggcaa ttccattata aaatcgtgtg cacctactaa tttgctggct
     7801 tgcacgactt ggtcatagct tatcgcagga ttgcgcagcg tgatattgtc gagcactgta
     7861 ccagaaaaca agaacacatc ttgcaacact gtgccaatgt atgagcgcaa tgctgaaagc
     7921 tcgtactgtt tagtattaat tccatctaat aaaattgcac cactattgat ttcataaaag
     7981 cgattgagaa tattaatcaa tgaagattta cctgagcctg tagcaccaac aatagctagt
     8041 gtttcgccgg catctacttg aaatgttatg tttttcaaaa cataatcttc tttattgtat
     8101 gcaaaccaaa cattttcaaa tgcaatatta ccttgtatgt ttgtcggtgc atgtttgcct
     8161 atattctcta ttttttcatt tgtgtctaat aatttaaaga cacgctccgc tgcaataatg
     8221 cccatttgca aagtattgaa tttatctgcc aacatacgca atggacgaaa tgccaaattg
     8281 agatataaaa taaaagaggt gaacacacca atagttgcaa agtcgctcat gacctgatgt
     8341 gcaccatacc aaacaagcaa agaaatagct aaggctaata ccacttcaac aacgggaaaa
     8401 aatatcgaat aatacaatac tgatttatta ttcgcacgat aatgttcttg gtttaattta
     8461 tcaaatttat tgaattcgtg ttgctgtgca ttgcttattt gcacgatgcg catgcctgaa
     8521 atatgttctt gcacaaaaga atttaattgt gctactttat cgcgaacctc attaaacgaa
     8581 acacgtactt tttctttaaa aatataggtg gcaataataa taatcggaaa agtaataagg
     8641 catactaatg ctaatttaaa gttgatatac aacataaatc caacaacaaa aatcagtgtc
     8701 aatacatctg cgacaatggt aattaatcct tctgaaaaaa tattatttat cgcttcaata
     8761 tcattgatag ttcgtgtagt aattatgccc acttgtgttt tatcaaaata ttgtaaacgc
     8821 atggattgca catgtgcaaa taatctagtg cgcaaatcca gcattatcgt ttgtcctaat
     8881 gttgtagaat ataagccgaa gagatatttt acaacaaact ctactatcaa caaaatcaat
     8941 agaattaagc ccatcatttc caagcctttt ccttcttgtc ttattatcgt ctcatctact
     9001 gtaagctgaa taaaataagg ccttagcggt gtgatgacgg caactaaaat tcctaatatt
     9061 acagacaaaa acaagagtct tttaaatgga aatgacaatt gcacaacacg ttttaatgta
     9121 tgccaatcac tagctttttg ttttgtgctc atgctttatt gtcttattta ttgattgctt
     9181 gtatcgcctg accatagtca atattccaca aaaataaacc ttttgcagga actgctaatt
     9241 taacgtctaa tggtttttgc tgtaatacgg cttcaatgag cgttgctgct tgcatttgat
     9301 tggtgcctag cagtaacaaa gctgcagtaa tacgacgcac catattacgc aaaaaacgat
     9361 tggcttttat cgtaaacact aaaccattat ccgtgctttg ccaataggct tcatataaaa
     9421 tgcagttcga tgttttattg tcgctgcctt ttttttcaaa tgtagaaaaa tcatgttcac
     9481 cgatgagttg ccgtatgcaa ttattcattt tttcaacatc aatttcttta ttccagcaaa
     9541 agctttggtc ttcaataaat ggatttttct gaaagtgtat aaaatattta taggttctgc
     9601 ttatcgcatc aaatcttgcg tgcaaatcag gccttacttc gattatttgc tgaattgcaa
     9661 tgtcatttgg caacatgcta ttaatacgat atacaaaatc agacattggg ataacatctg
     9721 tatcaaaatg cgcgaaatat tcctttgcgt gcacgcctgt atctgtccta ccacagccag
     9781 tgatggctat cgttcttgca aatagtttgg ataaatttgt ttctagaatt tcttgaacgg
     9841 aaatcgcatt ctcttggcgc tgccaaccgc aataattttt gccattatat gataattgta
     9901 agaaataacg catgcacttg acaaatagct tttcagatac aagatagttg atttaactaa
     9961 gtaaaaattt aaatccttgt tttatttttt ttaaatttga acttgcattt tatgcaatca
    10021 tcacaaaata aacatcaatt tatgatacaa cgcattcaaa ccatatattt actcttagtt
    10081 tctattgctt gcatagcatt tatttttatt ccttttggca ttgctacttc tgctgaaggc
    10141 attgctgaaa cggttgcagc taagcaagta attccaatta tggcaacatc aattgttata
    10201 gccatcattt cgttgatttc tatttttatg ttttctaaca gaaaaaagca aatgaaggtg
    10261 gtgttaatca atattatact ttcattggtg ttaataggaa tactgatttt cggaataaca
    10321 caacacatcg gcatagcaaa ctattctttt aaaattggta gcattttgcc catttttatt
    10381 ttattattta atatgttggc gtatggtagt attaagagcg acgaacaatt agtgcgctcc
    10441 atggatcgat tgcgttaatc aaaatttcca aatttcaaca aatccatctt tattagtacc
    10501 acacacgatg gccttgccta tgtgtatttg ttgtaggttt ggcgtggccg tatagcgcgt
    10561 ttccaaaaaa gtaagctgat cataactata cacttcattt ccttttgcat aatagatttt
    10621 ggaatcgata atctggatat ttttgaggct ttctatcggt atgtttttga tgaaattacc
    10681 ttgattgtcg aaaacaaaaa tgccgttttt ttcatcgagc aaatacaaca aaacgcctga
    10741 ttctattaac tgaattggct gtaatatttt ttgtgttaac aatgaaagtg tgttgctttc
    10801 aaaaattggt gtatggtttt tatctagctt cactaaacgt tgcgatgcgg catcatacag
    10861 ccataaaaaa ttattgtcat acgaagagcc aacacatgaa accagatacg ttcctaaatt
    10921 attgacatcg ataatgtcaa tttcttttaa gcgattatct aactgaatta ttttaccata
    10981 atctggcgaa aacaaaatag ttttaaaact attaacacta acaatttctg tgtgttcgtt
    11041 gatgtagatg ctaccataat tctgaaaaaa aatgccgtcg gcagtatatc gttccaacat
    11101 gcctttttgc aacaaaagaa tatcattact tgtattcaca tacaattgca aaggttttct
    11161 gtcaatcgtt tttacataaa ccggtgcagc agaaaaaaca gaaaaagtat tccagaaaaa
    11221 caacaaaagc atagctggtt taaagcaagt aaaccaataa aaacccaata ctatttcttg
    11281 ctttttactc attcgaatgt atttaatttc attgcattgc catcaaaaac tgcatattga
    11341 tgattcacta tccaatcgcc taaattaatg tatgtactct catctgacag ctttatttcc
    11401 ataggcaaat gtcggtgtcc cattacaaaa taattgtatt gttttttaag caacttccgt
    11461 ttacaatact gaatcaacca ttctttctct tcacctttaa acacttcaat tctcggatca
    11521 gtaaaagagt gcctcgacca ccatttagca atttttgtac caatatctgg atgtaaccaa
    11581 cggaacagcc attggcaaat cgaattggta aaaatcttct ttaaaatctt gtatttgcta
    11641 tcatttggac ctaagccatc accatgtgcc actaagatag atgtagctgc tttgtctctg
    11701 tttgggttat taatatttaa ttctatttta tcttgaaata tagtgacgcc aatttcttgt
    11761 tgcaagtaat ctttcagcca taaatcatga ttgcctgtga ataaataaat gtcgatacct
    11821 ttttctttta gctgaattaa tgttgcaaaa aatcgcacga aatattttgg aacaacatgc
    11881 tgatattcga accaaaaatc gaaagtgtcg ccaacaaaaa aaacggcttg tgcgtcgtgt
    11941 tctattgatt gcaaaaaacg aattactttc cgctctcgag ccaaactatt ttcgtaatca
    12001 ggtgcaccta aatgcaaatc agataagaaa tagatttttt tactagttga tatttgtagt
    12061 gtcagtgatt tcaaaaaaaa attttacaag caataataaa ttatacgttt gacgcttgtg
    12121 tacaaatcgc acaataaata tacttataat tctttgtttc tgttgtgtat taatgttgaa
    12181 tttcgtaagc ttgaatttta tctttcatgc tcaatagata atcaatatca tcatagccat
    12241 tcagcaaaca tgttttttta tatggattga tatcaaaaga tgctttttct tgtgtagcta
    12301 caatttcaat ttcttgattt tctaaatcaa ttttgaaaac tgccgcatta ttattttcta
    12361 ttgcagtaaa aattttctgt aaaaatgctg gtgatacaac aaccggtaac aagccattgt
    12421 ttaatgcatt attccgaaaa atatctgcaa aaaaactcga tacgactact ttaaaaccat
    12481 agtcgtgaat ggcccacgct gcatgctcgc gcgatgaacc gcaaccaaaa tttttgccaa
    12541 cgaccaatat ttttcctgtg taatttttat tgtttaatac aaaatcttct ttcaaagaat
    12601 ggtcagcatt aaatcgccaa tctcgaaata aattatcgcc aaaaccatcg cgtgttgtgg
    12661 cttttaaaaa gcgcgccgga atgatttgat ccgtgtccac atcttccaca tttaatggca
    12721 ctgctgatgt ttgtattgtt gtaaattttt ccattttttt gtttttaaaa ttggtcgcca
    12781 atcattcctt tacattaaag aacgcacgtc tgttattttt cctgttaatg cagctgcagc
    12841 agccatcaac ggacttgcca ataatgtgcg tgcaccttgt ccttgcctac cttcaaaatt
    12901 tcgattggac gtggaaacac aatattttcc ggctggaatt ttatcctcat tcatacctaa
    12961 gcatgccgaa cagcctggct cgcgcaatgc cattcctgca tcttttaata ttttatctaa
    13021 gccttctgca atcgcttgtt tttctacctg tttgctgcct ggcactatcc atacggttac
    13081 attttctgct ttttgttttc cttgtataag cgagcaaaat aagcgcaagt cctccattcg
    13141 cgaattagta cagctgccta aaaatacata gtcaatggtc ttgcctatta aggtttgtcc
    13201 tttttgaaaa tccatatatt gcaaagcttt ttccaagcca gcatcatcgt tatttggaat
    13261 ggtgtctgag atttttatgc ccattgctgg attcgtgcca taggtaatca tcggctcaat
    13321 atcagcggca tcaaaatgat attccctatc gaaaacagca tcttcatctg tttttaactg
    13381 tttccattta tcaacgcgtt cctcaaaatt ttctgcactg aatttcttat ctttcaaata
    13441 tgcaaatgtg atttcatcgg gtgcaattat gccaccgcgt gcgcccattt ctatgctcat
    13501 gttgcaaata gtcatacgcg cttccatgct caaacttcta attgcagagc cagcatattc
    13561 tacaaaataa cccgtgccgc cactcgtaga tatttttgaa ataatgtata aaattatgtc
    13621 ttttgaagta acaccttttc ccaaaatacc atcgacgttg atgcgcattt ttttaggctt
    13681 ggtttgcaag atacactgtg ttgctaaaac ttgttctact tccgacgtgc caattccaaa
    13741 ggcgatagca ccaaaagcgc cgtgtgtaga agtatgcgaa tcgccacaaa caatagtgat
    13801 acctggttgc gtaatgccta attctgggcc aatgacatgc acaatacctt ggaatggatg
    13861 gcctaagcca tagagctcaa tatcgtgatt tttgcaattt tcaattagtg tttctacttg
    13921 cttgcgcgac agagcttctt ttataggttc cagctggttc tgtgtgggta cattatgatc
    13981 tgcggttgcc acggtttgtt gtggcctaaa taccttaatg cctttgctat ccaatcctgc
    14041 aaatgcttgt ggtgtagtta cttcatgaat aaaatgtttg tcgatataaa ttacctgcgg
    14101 tccgttctcg attgcctgca caatatgtgc atcccaaatt ttatcaaata atgttgttgc
    14161 cattctaaaa attgaacgga caagttaata aaaattggtg gtgcctgttt tacagaagta
    14221 gttaaggttt tattaaggta aaaccattgt gcgttttaca tatataaatt gaagaaaaaa
    14281 aataagattt tgagttttga gattataaaa aaaaggttat tgttatttat tactagctct
    14341 acgtattagt ttggatttct attgtcattt ctataaaaga aaaggattgc tgacgcaatc
    14401 cttttcttta ttaataattt tatgttacca ctgcttagta gtcgtactta atccttgata
    14461 tagaaacaca atatctgtat atgtacctgt cagtgtgttt gcatcgccac tttcaaactt
    14521 atacgcaccc ttacctagta ttgaatttct cataattgtt gagccatttg gtttgtaata
    14581 acccaaatag ccagttgcag ttgtttcggt agtattgcct tttattaaaa agtaatgttc
    14641 tccgattgca tgagtaggca atgctgttcc tacgtctgaa aatccagcac tagaagatgt
    14701 acttgaaatt tttacttttg cacctgtact gctagctgta gcagtattta tcttgactgc
    14761 aaacagtaga cttccagttg gtattgtatt accatcatct tgagcaatag tccatgcaga
    14821 tggattggtt gcaaacgctc cggtacttgc agtaatataa tctgcttcat tagtccctga
    14881 acgtgttaca tttgataatg aactagctag actattatac tctgtgctag ttatctcaat
    14941 ccatgtgccg tttgcagcag cttcataggc tgcttttgat gtgctaagat ttgcaagtaa
    15001 tttgtccgtt tcttcttttt tacatcctgc taatgttacg aagacaatag ttaggaagat
    15061 taatgatttg ttgttcatag agtttatttt tgatttattt aaatgtaata ctttttttgg
    15121 aatctatttt aaaaaaataa attgcatttt tttgcaattt tgaatatgat actgctagat
    15181 tttgtttata tttaaagaac aaaatcatta attatgaaaa acatattctt ttatgtgatt
    15241 gcatttttta tggtaattag tgcaatttct tgcggaaaaa aaagcggtac ttgcgtatgc
    15301 aagcttagtg gacttgaaat cagtcgtacg cctggattaa gtagctcaga ctgtaatagc
    15361 cttgagcgtg gcattcctga tttaaattgc tatatagaat aaacgactcg tagccaacta
    15421 acttaaagat aaaaactatt tctttgccaa aaaaaacgta tcttttctgt ctaatttatt
    15481 cagatggaga taccaatttc aggttatgta aaacctggtt ttgaaaaagt aaaaacaatt
    15541 ttcgaagaga attttgctaa aagaaaagaa atcggtgcct cctgttgtgt atatcacaaa
    15601 ggagaaaaag tagtggattt atggggtggc tttcgcgata aaaacaaaaa gcaatattgg
    15661 gaagaagata cgctttgtat tgcattttca acaaccaaag gcatatcatc gcttgccttt
    15721 gctttgttgc atgctaaagg tttaatagac tacgaggaaa aggtttgtac gtattggcca
    15781 gaatttgcaa gtaatggaaa agagcacatc accatacgtc aattactatc gcatcaagca
    15841 ggcttattta cgaacgacaa aaaattagat tacgctgtgc tgaccgatga gacggcattg
    15901 tcaaattgtt tggcaaaaca gaaaccctac tggaatccgg gcgacaaaaa aggctatcat
    15961 gcttggacaa ttgccatgta tcaaaatgaa atttgcaaac gtgtagatcc cttaaaaaaa
    16021 ccacttcata tattttttaa aaatgagata gcagatgcct tggatatcga ttttcatatc
    16081 ggattaccag aacacattcc acagcatcga gtggcacaat taattccttt ttttccaact
    16141 gaagcattaa acggaaaaaa ttctgattta ggcacaatta tgcgtggtgt tttaaatcca
    16201 ttttccctgt tctataaatc attattgaat ccaccacacg cactcaactt aaacaacttt
    16261 aataagcgca aatttgtatc cataccgaat gggtcggcac atggatttgg aaacgcgcgc
    16321 tccatcgcac agctctacaa tgaatttgca acaggtggca aaaacttaca cttaccgcgt
    16381 gcaacattaa acttattgga agcggctcct attcatccaa aattaagcag aaaagatctt
    16441 gttttaggcg tagatattcc attttcatta ggctttgcca aaccttcgat attccaaaat
    16501 tttggagtaa actatagaag ctatggttcg tttggtgctg gcggttctgg tggttttgcc
    16561 gaccctgaaa aacaaatagc ttttggctat ttgatgaata aaatgggaac acatattgcc
    16621 aatgatccac gcgaactcgc cttgcgaaat gctacctaca catgtgtttt tgacatagaa
    16681 agaaagggat aatatttttg attttgctga aaaaattgct attgatttgt tggtgacacc
    16741 aatatcggct aatgtattct ttaattacaa atgagacttc ccttacattt gtatgctgat
    16801 aggcggaagg aaaaagcttt cgatgtcatt accgcgcaag cgataatctt tgcctttgat
    16861 ttttttgcct ttgttggctt gcagccaaca aacagccaag cgaaatagaa agcggtttgt
    16921 tggttgaaaa ccaacaaaag cgatgagaag tcattctcac gaaagtgaga atctcatttg
    16981 tcaatatgtt ggattcgtga ggacacgaac catggctaag gccttgcttt gtgcagatgt
    17041 gtacttgtgc gagttgtata aaaagattaa atctggatgg aaaataatat aagatgagtt
    17101 caacaataga tacattaaga gaaaattaca tcattattta gaagtagtgg aagataaaaa
    17161 aattcatgct atctatactt tatttgaaga agaaataata aacatagaac aatataataa
    17221 agatatagaa gacgctgaag aagaaataga tgcaggtaac tattatacac atgagcaagc
    17281 attggcagaa ataaaaacat ggaaaagtaa atcggtatga tagaaataat ttgggcgaaa
    17341 agagcattaa aaggattgca aaaaatatat gaatacatag cggaagattc gccacaaaat
    17401 gcagaaaaag taatcattga aatagcagaa aaagtgactg gattaaaaga gcaaccagat
    17461 cgatataaac tagatgactt tgcctttgtt ggcttacagc caacaaactg ccaagcgaaa
    17521 tagaaaacgt tttgatggtt ttcaagcaac aaaagctgtc ttgcaaaata taaaactcga
    17581 atagagcaag aatttaacaa gcttttataa actatcagca aagtttaact ataaatacca
    17641 ttattattca actgaaagaa ccccaaattg attatatttg ttgtaatatt tttaccatgc
    17701 gcaacttata caaatttggt tttttcttac tttcaatgtt tctgttttta ggaaatataa
    17761 atgcgaatat ttatcccaat gaccgctcct tattagactc cacgatacag cctttttacc
    17821 atggtgttgc ttctggtgac ccattaaacg acagagtgat tatatggaca cgtgtgacga
    17881 ccgaccaagt atcggtgcca gtaaagtggc gtatggccac agatacaggc atgcaaaata
    17941 ttgtagggca aggtaccttt acaacagata gctcgagaga ttataccgta aaagttgatg
    18001 tagtcggctt gcagccaaat acctattatt actacgagtt tgaagcattg ggcagacttt
    18061 ctcaacgcgg cagaacaaag acaatgccga caggcagtgt caataattta cgaattgcat
    18121 ttatgagttg ttccaattat tcttttggtt acttcaatgc ttatgatgcc atcactagac
    18181 gaaatgatgt ggatttattg attcatttag gcgattatat ttacgaagat ggcttgaaaa
    18241 caggacctta tgtggttgat acgataaaac gtagaacttt tcctgaatat gatgcttttg
    18301 atttagtgag ttacagacaa cgatatgctt ggtatagatt agatccatcg ttacgtaact
    18361 tacatcagca gtttcctatg gttgtaattt gggacgacca tgaatttgcc aacgacgcca
    18421 caaaagatac tgcattaagg catgacccag taaagcaagg cccatgggct gtgcgcaaag
    18481 caacagcaat tcgtgtgtat aaagaatgga ttccgatgcg cgaagacaca tctaacacca
    18541 atattatcaa ctttacgcaa cccattggcg acttagcaga tataatctat acagaaaata
    18601 gaatagagcg cattgatgcg catagttttc aacaagctta tgatttggtt tcgcatatag
    18661 ataatttgcc ttatgacaca ccagatagaa ccatgcatgg ttttcggcaa atggaatggc
    18721 tgacaaatca attaaaacgc tcgacagcaa agtggaaagt gatggccaat caagtggtat
    18781 ttgcttctta tgtatataag caagccattt taggtattcc atttccattt cacaatgctg
    18841 caggctggga cattaatcct ttggatagaa agatggtatt agatactgtc aatcggtata
    18901 atcttcagaa tttagtagtg ttgagcggcg acatacatgt gtcgatggct ttcgatttgc
    18961 ctggtggcgc aattccttat gatccagcca ctggtgaagg ttccataggt gtggaatttg
    19021 tatgcgataa taccgttcgt ggtggtttgt acggcggcca acaatcctat atgtacacaa
    19081 ataatcaaca cttgaaatac attaatccga attccttagg ctattgtgtt ttagatttaa
    19141 cacctacatc agcatgttgt gattattggg aaacagatac cagcggaaaa ttaagtagtt
    19201 ccttgaaatt attgggcact tggtgtacta aaataaatga gaatcattta aaaccatatc
    19261 cagggccaac atatacatct actgtatttc ctgccttagt gccttacacg ccgcggaata
    19321 atggcattac agtaggtgtg aaaaacacac aaagagcggt ggaattaatg agcgtgtttc
    19381 ctaatccaac acaacattat ttgcgattcc aatattttat taaagcacca gaaaatcttg
    19441 ttgtcaccat atatgatatg ctaggcaatg cagtcaaatc tgtagattat ggacagcaag
    19501 gtgtaggttt gcatcaagac ggatttccaa ttatggattt agcaccagca gaatatatta
    19561 ttacattcca atctggtaca gacatcgtat cgcgaaagtt tattaagcat tagcagaagt
    19621 gatatcgatt tcaataagct taaaacggaa tatgtttttt taattgaaga taaatacttt
    19681 tcttttttgc taaagcatgt ttttctacta agtgccacga caaatatcca aataacaagg
    19741 tgagtggcaa cgaaaccaaa agcaatccga taacatctaa ttgaaaataa tggagcaata
    19801 attgttgaat aggaaaaccg tagatatatg ttccatatga tacatcacca aatttttctc
    19861 ctattgatga aaatccattc cacgccatca taccaataat aattactatt ggaggaaaaa
    19921 taaaatattg aacgatttca atatatatat aatttttttc attactttta ttacttgaat
    19981 tcagcaattt tctaaataaa ggatatgtat aaaattaaac tgaaaatatg catatgtgtt
    20041 tgtgtatatt taattcataa tgcaaaatct caatcgattc cagctgactt aggattaggt
    20101 tcaaaagtag tatggaataa agatttaatg ggatttaatc ttggtgattt ttcaaatact
    20161 aatgtaccag acaataatat atttgatatt aaaaataatg gtagtggaaa tacttataac
    20221 aactatacaa gtttaattga agatgcgggg ctgggaaaaa atatattata tcgattttat
    20281 accggagagt ttggtaattg ttacaatcgt tggtatggtg gagctggtgg cacatataaa
    20341 gattcattag ggaatatgat ctttgatcct tattcttcaa catttcatca tattcaatat
    20401 aaccaattta attcaaattt aaatgatagt ctaagagata atttatacaa taattatttt
    20461 acatataatg atacgccatt tattacctat caaacaactg aagttgcaaa acctaagaac
    20521 aatattattt tcccatttat aaaacttgtg accaacaaca gagccaaaaa tgcaaagact
    20581 gtgttcaact tacctattac tgcgcactac agagattata gaacaaataa ctcttggaaa
    20641 tctgatctaa ataataataa ttttgcgctc actataaatt tttacgctaa attaatagga
    20701 gtattttcac aatatgcaga caattactct ccgcggaaag aaacattttc taagtcatct
    20761 gttattgcgg aaatatacaa tatgaattca ttaaacactt ctgtaagcaa tattattcca
    20821 acgccttcaa ataaaatttc accacaattt aaagagattg tattacaaaa cttagcagct
    20881 ttttctacat taatacaaaa tcgagtaaag gtggtacata ttgaattagg caatgaatta
    20941 tatagtagaa tgtataatgg agggtattgt tcgtggtggc aagctaaccc aagttttaca
    21001 gaattctctg acaaaacaaa taataacata tttaatgatg ttttatttta taatgatcct
    21061 tgtaataata cctctccttc ttcaacatta gcaaaatcag ttaatgttta tggaaattta
    21121 attaagatgt atatggctct tatacgagaa ttagttgaaa atctatggag taatgaatca
    21181 gatgaaataa aaaaagcatt ctatttagat ataaaaaata atttaaaatt tggtgttcca
    21241 atgtctgggc gttttccaaa tctaagttct aatggttttg cttctttaga taaatattta
    21301 tatgataata aatctacact aggatttgat gcatttattc agcacccata ttataaacct
    21361 aatatttata atggaacact tgacgtaaaa tatgattcat taaaaaaata ttattcagaa
    21421 aggtttaata catactataa acaaactttt ccgaaattaa tacgtacaaa ttatccaaat
    21481 atttccgaat tatggtttac agaatgggca catgaagacc caaataatat taataataca
    21541 ttacttgagg gaatgaacta tattgatgaa tttatgaatt tttgggattt aaatagcaac
    21601 cttaatttga cagatactct ttttagaaat aatgatattt tgaaagttgc gaatcatcat
    21661 atgctttggg ataaatatgc aagcaataaa caactgataa atattcaata taatcaactt
    21721 ttacctaaat atagaacaat gttttatgct cataaattgt tattaccaat aatggataat
    21781 aatactagaa tgataaataa acttaacgga ggatttgtaa gtccaaattc agatatctat
    21841 tttaggtcat ttgagtcacc acaatcaggt caagatgtac caggtaaacc tggctatgag
    21901 ttcgcaaata aaatatacat ctattataat aataaaaaca ataatactac tccatatact
    21961 tttaatctta atagtgcagt tcagagtcta cttgctaatt gcttcttttc tactcaaata
    22021 tattcagtaa agaggaacag tatatatgca aataaactat atatatccag aggaaaaggg
    22081 ccttcagaat cttctgatac aattacagat gatccgaaca aaacagttca atatgagtct
    22141 gacttattga taaatgatat aaattcatgg aatacattgc ccatagctaa atattctgca
    22201 ggttattacg aaattacaat aataagggaa actagtattg gaacaagtgc ttcatcaaaa
    22261 agctataaaa acaaaggtta ttttattaat agtttatttc ccaacccatt aaatagaggt
    22321 gaaaaaataa atattaaagt taatggtgaa cacgatttag atgcaaaaat ttacatcagc
    22381 tcaatagatg gcaaactttt aaaaacaatt gaatatccta tacaaaaggg agaaaattac
    22441 ataaagataa attctgattt tctgtcgaat ggtatatata tacttcatag tgatgactta
    22501 tcaattattg aaaagattat tgtaaaagaa tagcaaatca gtcttttttt tgataaacaa
    22561 atattcgaac ttgtaatgtt gtctatattg atgtaaatta gctgacataa catgttattt
    22621 aattcaatta gttatctagt ttttttacca atagttttca ttctttattg gtttgtattt
    22681 agtaataaag taaagaccca aaatttatta ttattacttt caagctattt tttttattct
    22741 tgttgggatt ggaggttttt gtttttactg atattctcta cagtgcttga ttattataca
    22801 ggaattaaaa tacaacaaaa ttcatctaca aacaggcgaa aatattggtt ttggttaagt
    22861 gtttgcacaa acttaggttt tttaggaatt ttcaagtact ataatttttt tattgaaaac
    22921 tttattcaat tgttttctga attaggtatt caaatacatg ctacaaccat aaatataatt
    22981 ttaccagtag gaatttcatt ttatacattt catggcttat cttatgtgat tgatatatac
    23041 aagaatagaa ttaaagcaga aaataatttt atagattatg cagtgtttgt aagctttttt
    23101 cctttgttag tagctggtcc aatagagaga gctacgcatt tattgcccca aataaaaatt
    23161 aaaaggattt ttgaatataa gtatgcaaat gaaggattga gacaaattct ttggggttta
    23221 tttaaaaaag tagtaatagc agatcaatgt gctcaatatg cagatatcgt ttttagcaac
    23281 tcaaatattt attcaggaag tactttgcta ttaggagctt tcttctttac tatacaaatt
    23341 tacggcgact tttcaggata ttctgatatt gcaattggca cagctaaatt gttcggaatt
    23401 gatttattga aaaatttttc atatccatat ttttctagag atattgcaga attttggcga
    23461 cgttggcata tctcattatc atcgtggttt aaagattatt tatatattcc attaggcggt
    23521 agcaaaggtg gcacatacgt taaagtgagg aatacattca ttattttttt agtaagtggt
    23581 ttttggcatg gagcaaattg gactttcatc atttggggtt tattaaatgc actttatata
    23641 atgccatcaa ttatttttaa tacaaacaga aataatttag aaatagtagc taaaggaaga
    23701 ttatttccta ctataaaaga atttatttca atttgtatta cgtttatgct tgcactattt
    23761 gcatggattt tttttaggtc agacaacttg catgtagcat gtgattttat aaaaaatatt
    23821 tttgattata gtttatttgc aattcctcag gtaaaagaca tgagattact gttgacgata
    23881 gctatattct ttagtataga atggatagga cgtgaaggca gttttgcttt agaatactta
    23941 gaaaaaagat tgcccttact tcttagatgg atattctatg gcattctaac tgcaagcata
    24001 gtgagtcatg tcggaactga taaacaattt atttatttcc aattttgaac aaattatgaa
    24061 gagattttta aacagattgg tttgttttgt atgtatatta ttgtttgtaa ttacagtagg
    24121 gatatttttg ccagcaactc caagagaaaa aaaatcacta ttgttctcta agattgctaa
    24181 agattccctt ttaaaagtca caaaatcgcc aagaataata tttattggcg ggtctaatct
    24241 tagttttggt ctcaatagcc aaatgattaa agattccctt cattattttc cgataaatac
    24301 atcgatacaa gcgtctttag ggatagtata tatgatggat gatgtttgga agtacctaaa
    24361 gaaaaacgat gtagtcatat tagtacctga atatgctaat ttttatggca atttcgcata
    24421 cggaagcgaa gaactactaa gaatagaatt agatatttat ccaataaaaa atatttcttt
    24481 aaggaagaaa caatggctta atatagctaa gtttattcca aagtatgcat tctcgaagtt
    24541 cagaccatct gaatatataa atatcaaaga aaacgatatt tacggtattc attcatataa
    24601 ccaatacggt gatgtcgatg cacattggta taaaaaatca gtaaagtatg agccaattac
    24661 taaatttcat ggcgctttaa atgatgctgt ttttaattca atagaaacgt ttaacaataa
    24721 acttaagtcg agaggcattt tgttctacgt gtcatatcct tgttttcaaa aaacatcata
    24781 taaccactgt gctaaacaaa ttaatgaaat agaaaaaaaa cttaagcaat cgcaaattaa
    24841 tattttaggt tcgcctgata aatattgttt tgatgattca ttaactttta ataaaccata
    24901 tcatcttact aaatcaggat tagatagaag gacacaattt ttaattgaag acctaaaagt
    24961 agcaatgaaa taattatatt tgcaaaaatg catttgctta acattaagaa aattaaactt
    25021 attaatgaac gcaactatct aaaattagat gcattcacta ttaccggaat ttgcttatta
    25081 attttttgtg gtattcgtgc caccatgtat ttagaaaaac acatggatat tttgttttgg
    25141 gacgaatcgc tttatctaac acgcggactt tccatgttta aatacattcc acgcgattgg
    25201 ggtccgtcct attcattatg gtacaagttt ctgtcctaca tcttttccga tagagttgaa
    25261 ttgtactatt ttaatttcaa actcacgact attttggttt ccgtgtcctt gtttttgcta
    25321 ctcttgtcct gtggcataca acgtatttta gcatttttat tttccttact ttttttatcg
    25381 tcatatatta atattgctac gtggccacgt gtttctcatt tttgtattat tgtaatttgc
    25441 gcaggtataa ttttggcaaa aagacagcac acaaaccttg caaagtttgt gtttctaagc
    25501 gtagcttttt taatatgttc cttcgcgcgt ccagaactat tcttaccttt tttactttgt
    25561 ttttttatta ctgctttttt gtttttttat aaaataaaat caagaacaaa gtacgaatat
    25621 gtattaatga tgagtttgat tgtgtttctc gtatttctgt atatattttt taaaacacca
    25681 ttcaataatg gcgacaccaa tagaggcatc ggcgtgtttt tgcagcattt tgcgatgaac
    25741 tacagccaat ggaaacatga tggttccatt ttttggctgg actttccgac catcttaaaa
    25801 cagaattttg gcaatgctca atcattagga gaaatgtata aagccaatcc agctttaata
    25861 cagcaacata tcgtatctaa tgcaacaaat tatttcacac agataggaaa aataatctta
    25921 agtttttttg tgccattgtt tacaaagtct atcaattggc tggctataat gatggcattc
    25981 atgttatttg tagtatattt ttcttataca aaaaatatca gcaataaacg gcaacgcatt
    26041 tttctattgt tgaaagataa tacgctcacc atttttgttt tattgctttt cgcaatgcct
    26101 tctgtttttg tttgctttta tgcatatcca cgtgaacact atattttatt gcaaacgcca
    26161 ctactcttac tgataatagc tatcattttt tcatctattg ctgttgctat ttttaagact
    26221 actcaaaaaa tagtggtgat tgcagtagtt tggttttttg taatgcctac ggctgctgat
    26281 ttttcttatt ttcgaatgtt tagagcagca gataatttat ccaacaaaca aacagtgtca
    26341 tatattaaaa aacactttac aggaaaagac agcattcacg tttttgactt agaaggtggc
    26401 ttgacgaatc tgcttgcacc aaattttaca aattctaatt atttatactt acaagacaga
    26461 gaaaaaatta aattaagtgc tttcttgcaa cagcatcaat atgatatcat ttataagaca
    26521 cctattttga cacaattaga agctacaaaa tcagatacta gcttgttcga tatgttgcaa
    26581 catcctgaaa aatatggtta ttttgaacaa aaaactggta actttgccaa tagcctattg
    26641 attaagcact aagatgaatt tcgcttataa aacactttat acaagagatc agccactttt
    26701 tgaattgaaa gtagcaggta tctttatatt tagcttgttt tttgcaagta atatcttaat
    26761 tcaatttaat tcagcgagca aagtagctgg tatcgtaatc atgttgtttt tgccatttat
    26821 cttgatatca gctggcatat actattacta caagatattt cagttaaaag aattaaatcc
    26881 gatagatatc attatcctac tttccttgct tttgccattt tataatgcta ttaccgtaaa
    26941 tatgattttt gatgtaaaaa tcatgaaagc attatttaac ctttcatcga gattttatgt
    27001 agtaatgtgt agtttgattt attacgcaat taggtcaaat aaaattactt taaaacaata
    27061 tttatatgcc aatcttcttt taagctggtt ttgtttcgcg ctatattctt ttgtatcatt
    27121 aaccattcca ccagagacat ttatcagtgg cgtaggtgat ggattggtgg gctataatcc
    27181 aagtaaaggt ggttacatct atcgtttttc cagtgcgcta atcgtattcg gaatgattta
    27241 ctatttccta gattatatcc aaaaaaacaa cattttaagc ttgttttttt ggatgatgtt
    27301 tttggcatat caagtgttta tagacaaagg tagaattgaa ttagtgtctg tagccatacc
    27361 aatggttatc tacatggtct atttattaaa atggcatgat ttaattaaaa aagtgcttac
    27421 tattcttttg ctaattggtg tcgtgctcat tattgcatat ctcatcgatc cgaagattat
    27481 acaatttaca gcagacatgt attggatgtt tataaagttc tttatgggcg aaaaaacagg
    27541 cgaaggctct gcggatatgc gctggacaga aatggctgcc gtctataatt attttcttaa
    27601 atatcctgac catattttct ttggtattgg tacgccaaaa agtgagatat tacaaaaaaa
    27661 tgtaggtacc gttatatttg gcgatatcgg tattgttggc ggattattat cgcaaggaat
    27721 tgtaggttta atcattattt attcggtgtt cttatatcca ctttttattt ggtataaagt
    27781 gaaacattat aaacacgata tattctttaa tgtcggcgta ttaagtacga ttactgtgtt
    27841 tattcaatcg ctatttagtg gtgccatatt ttattcgttt ctaggcatta tgctaccatt
    27901 gattatcatg gattattatc gtgtaaaaga gaagaactac tggaaagaac agcgcagcaa
    27961 accgccagca tgaaaaaatc cataaaaata ataggaattg tatttttgat tgtttttgtt
    28021 gtaaaggtat gcacttctcc tgccaagaaa aacactgcaa caatactaca aactgctaag
    28081 gaagttgtag cagttactgt tgatgcagat tatacaactt caacattggt aaatccaaaa
    28141 atatttggtg tgaacattgg atttgcgttg gctcgcgaac tcgataaaga cagtggattt
    28201 gtacagcttt tgcgcgaaat gcgtcctcat tcattgcgct ttccaggtgg cacggtcgcc
    28261 aattattacc atccgcactt gcctgtttat ggttataaat cgaatgaaat tccacatgga
    28321 ttgggcgtgt tatataaagt acaatttgaa cggaaggaaa atattttgta caactttatt
    28381 cgattggcaa aacaagtaaa ttgtggtgct gttttttgtg caaatgtatt gaccggtact
    28441 accgcagaaa ctttatttgt gctcgacgag ttaagcaaaa ataatattcc aatttatggc
    28501 gtagaattag gcaatgagtt ttgtttattg ccttatcgca aagacgtgtt tgtaacagag
    28561 caagtatata tcgacaagat aaaaaacacc gctgctgcca ttcgccaaaa atatccgaat
    28621 ttgaaaattg ccgtagttgc tggtgtt
//
LOCUS       DAOQQV010000022        28380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_496_length_28380_cov_8.135004, whole genome shotgun sequence.
ACCESSION   DAOQQV010000022 DAOQQV010000000
VERSION     DAOQQV010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 28380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28380
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_496_length_28380_cov_8.135004"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLL59_04030"
     CDS             complement(<1..209)
                     /locus_tag="PLL59_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMY23006.1"
                     /translation="MEKNAKIYVAGHNGMVGSAIVRKLKAEGFSNILTASSKELNLIN
                     QADVNAWFEANKPEYVFVAAAKVGG"
     gene            complement(284..1396)
                     /gene="gmd"
                     /locus_tag="PLL59_04035"
     CDS             complement(284..1396)
                     /gene="gmd"
                     /locus_tag="PLL59_04035"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMY23007.1"
                     /translation="MKKAIITGITGQDGAYLAELLLSKGYEVHGIKRRSSLFNTDRID
                     HLYQDPHIDNRNFIMHYGDLSDSTNLIRIIQQVQPDEIYNLGAMSHVKVSFDTPEYTA
                     NVDAVGSLRLLEAIRILKLENKTKLYQASTSELYGKVQEIPQSEKTPFYPRSPYAVAK
                     MYGYWITVNYREAYGIYACNGILFNHESPLRGETFVTRKITRAVSKIALGLQDKLYLG
                     NLNAKRDWGHAKDYVEAMYLILQQAEPEDYVIATGKTTEVREFVRMSFKEVGIEIEFK
                     GTGIDEKGYIKSCSNEKYQVEIGAEVVAVDPRYFRPTEVDLLIGDPTKSNTKLGWYPK
                     HDLASLVKDMMEHDIKLFERDKYLLEGGHKIFNYHE"
     gene            1465..2037
                     /locus_tag="PLL59_04040"
     CDS             1465..2037
                     /locus_tag="PLL59_04040"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HMY23008.1"
                     /translation="MKLKIALFLSGSGSNARNICSYFKNHEQIEVSIILSNKENSGAK
                     HVSAEFDIPYFIFTRAEFYDTSIVAEELKKNKIDTLVLAGFLWLIPSYLLEQFPNQII
                     NIHPALLPKYGGKGMHGKHVHEAVFSNKEKESGITIHLCNKEYDKGEILFQAKVSLTK
                     EDNPESIAAKVLQLEHQHFPLVIEKYMLAK"
     gene            complement(2105..3892)
                     /locus_tag="PLL59_04045"
     CDS             complement(2105..3892)
                     /locus_tag="PLL59_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY23009.1"
                     /translation="METLEQIKSLKGGEFIIKESSTEQVFTPEDYTEEQLMVRSMASD
                     FVEKDVMPRAKEYEKQTPGLSLELLRKAGELGLLSTAIPESYGGMNQDVITGCIIAEE
                     FGRTGSFATTSICHIGIGTLPILYFGNEEQKQKFLPKLATGEWAASYCLTEPSSGSDA
                     LGAKTIATLSEDGKEWILNGQKMWITNAGFAHSFTVFAKIDGKEFTAFLVEAGTPGLN
                     LGAEEDKMGIKGSSTRQVFFENCRIPVENMLGERGKGHLIAFNILNIGRYKLGATALG
                     GAREAFNQAVQYASERTQFGKSLNTFGAIQHKIAEMAIQLFTLEVATYRCANDIGKLE
                     HENLASGKEFSKALLGAAEEYAIECSIVKVLGSEVLDFIVDENVQIHGGMGFSEETNA
                     ARAYRDSRIARIYEGTSEINRLLSIDMMLKRAMSGKIDLMTPGMAIQKELMSVPDFNS
                     DDEGQFAAEFKAIKNAKKAFLLVAGGAVQKLMAKLKDEQEIIMNTSDMLIQIYAAESL
                     LLRVQKIYEKGDKADDVYIDILKVFFNDAMNKINVFGKDALQSFAEGDELRIMLMGLK
                     RFTKYEPVNVKEARRRVAAKIIAGGKYNL"
     gene            complement(4088..4804)
                     /locus_tag="PLL59_04050"
     CDS             complement(4088..4804)
                     /locus_tag="PLL59_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HMY23010.1"
                     /translation="MKTYFLILFCISTQLSFAQVDSLFLPHTDSIQKSISNNKKWQWF
                     PPKRDTLNNKLILRNEKTNFGRKFLRAEGIVLGIQGTTMGILFALPASVSNWDKENIK
                     NYRQNYKRAFTQKPVVDKDHWYINYIGHPYQGSYYYNAMRSQGAKFWQAGLFSFGQSM
                     VWEYLLESGIEQPSIQDIIVTPVLGSLLGELFHFSSIKMAKNGYKWYEKAFISVFNPM
                     FAINNAFKFAKQVNVPMKTF"
     gene            complement(5294..6472)
                     /locus_tag="PLL59_04055"
     CDS             complement(5294..6472)
                     /locus_tag="PLL59_04055"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMY23011.1"
                     /translation="MNEAYIVAGYRSAIGKAKKGGFRFYRPDDLAADVVKHLVASVPN
                     LDPSRIDDLICGNAIPEAEQGMQIGRMIVLRAGLPLSVGGVTVNRYCASGLETIAMAT
                     AKIKAGMAECIIAGGVESMSLLPMTGWRTVLNYGIASTTPDYYSSMGLTAEAVAQQYG
                     ISREDQDLFSFNSHQKAIAAIESGKFKDEIVPVTVEEVYLQNNKRKTRSFVVDTDEGP
                     RKDTTVEGLAALKAVFAAKGSVTAGNSSQTSDGAAFVLVMSEKMVKELNIEPIARLVT
                     SAVVGCEPRIMGIGPVVAIPQALKQAGLKLDDIEMIELNEAFAAQSLAVIRTAGLNPD
                     IINPNGGAIALGHPLGCSGAKLSVQLINEMRRQNKKYGMVSACIGGGQGIAGIIERLK
                     "
     gene            complement(6504..6857)
                     /locus_tag="PLL59_04060"
     CDS             complement(6504..6857)
                     /locus_tag="PLL59_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMY23012.1"
                     /translation="MNNFKELIVWQKSRTLVKATYVLSEQIPNAEKFGLISQIRRSVV
                     SIPSNIAEGASRSSKKEFAYFLEMAYGSCFELETQLLLCVDVNFIKQEGSLELITNIQ
                     EIQRMIYKLIKIKKS"
     gene            complement(6887..9265)
                     /locus_tag="PLL59_04065"
     CDS             complement(6887..9265)
                     /locus_tag="PLL59_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HMY23013.1"
                     /translation="MSNRVIKKVAVLGSGVMGSGIALHFANIGIEVLLLDIVPFDLKE
                     EDKANPKMRNKIVNDAFTFALKSKPAQAYNSESAKRVSLGNFEDDMPKIKDCDWIIEV
                     VIERLDIKKQVFEKVDALRKKGSLVTSNTSGIPIHLMAEGRSEDFQKNFCGTHFFNPP
                     RYMRLLEVIPTPTTDQNVVDFLMHYGDVFLGKDTVLCKDTPAFIANRVGVYAMAKVFQ
                     LTQELGLTIEEVDALTGPAIGRPKTGTFKLSDLVGNDTGTKVMMGIKENCPNDEQANA
                     FVVPAYVQFLLDNKFFGNKTKKGYYYKDEKRNSFSLRLDTLQYEATGKPKFASLDASK
                     KSENLSDKVNAFIKADDKGAQLVKRSLAGLFAYVSNRVPEISDVLYAVDDALRAGFAW
                     EIGPFQYWDIVGIQKGIDLAEADGLSISAWVKEMIAAGNTSFYKVENGVKKYYDQQSK
                     SYKTIPGAESFIILDNLRANKPVWKNSGGTLHDIGDGVLNLEFTSKMNSLGGDVLQAI
                     NKSIEIAENGYKGLVIGNDATNFSVGANLMMISMLAMEQEFDELDIAVRQFQQTTMRC
                     RYSGIPVVSAPHGMTLGGGCEVTMHSDVVVAAAETYIGLVEVGVGLIPAGGGTKELTL
                     RVSDSIKNGDPILQTLQDNFLNIAMAKVATSAAEAFDFGLMRKNRDRVVVNGKRLIAE
                     AKQEVLRLNEKGYSQPAHRSDITVLGRTGLGTLTVGVESFVAGGYISAHDAKIAKKIA
                     YVMCGGDLSAQTTVNEQYLLDLEREAFLSLCGEKKTLERIQAMLTTGKPLRN"
     gene            complement(9428..10003)
                     /locus_tag="PLL59_04070"
     CDS             complement(9428..10003)
                     /locus_tag="PLL59_04070"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HMY23014.1"
                     /translation="MPKQKVSLDDILKHSLKLFRQKSYHNTSIADIAAACGLLKGSLY
                     HYFPSKEALMSAVIKYAHEYFQKEVFSIAYDVTMSPQQRMEKMFKKSERALLSEGNIM
                     GNIGVETARAIPEFAEHIRAFYTEWINAVAFVFKEITNEDDAMKLAEQTVAEFEGAVM
                     MSRIFKDTKYIKNAYERLVNRFAAFTLAELK"
     gene            10461..13526
                     /locus_tag="PLL59_04075"
     CDS             10461..13526
                     /locus_tag="PLL59_04075"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMY23015.1"
                     /translation="MFGLDVTQIRFDVIDIKQGLSQNSVSHIYQDSKGYMWFCTDEGL
                     NRYDGYSFLKLERIIGNENSLISNFTTCITESKPGIFWIGTETGLSVYLFDEKKFLHV
                     ENRGQITKIFVEDSFSVWIQTQYSLYKIRLVANNKSANDYTLNIEPLNALKSVICKLD
                     VNHFLIAGRDNKLYLYDIRTKLLQNKSKYDIWKIIIPKHINDVIKDRENNFWVATNAG
                     LYKVNSQQNSIDSFSENRKSIHGLNERITDVAVGKMGNIYIATAKNGLVIYEAEKNKF
                     NEYQSDPYNTTSLPDNKILSLLTDNSNTLWVGTKGAGIATFSQFKYKFKHITQEIFKT
                     TWLTNKYILSLVADKNENIWIGTDGGGLFKFNAKENIFSNWRYNILASSLTDDVVQEV
                     LFDVYDNLWVNTLNGICKYNPNNNKFTRYSLPYTKAKESISKVSTYSNVLLFSSKSGD
                     FYALDEQTIFKFNYLLNQFEVLPIPVANQNFIPRKIIEDIDNTWWLATSIGIIHIDLN
                     TGILQKDALNAINNKFFKNDQLYCLMQHDEDRIWIGSGNNGLFLFNKKKMQIEANYTE
                     KDGMSNNFVYGILKDKVGKIWMSTNSGITVFDPILKQFRTFDINDGLQSNEFNKGAYY
                     KTANNELLFGGINGFNIISPTNIPFNNYKPLANITGIKVNDSLLLPLAYSNQSKRVRF
                     ANSQNNITFEFASSDFANTPKNVFEYKLEGYEKNWTRTRRNHATYTKLSPGNYTFYVR
                     VSNNDGLWSAQPAAYSFRVRPLIWQTWWFKIIAGILFLLYAYQLVSNRIQAARKKEKE
                     KTQLAQQKAEFEKQLAEIKLKSLVAQMNPHFIFNCMNSIQAMILSDQNMQASTYLTKL
                     SRLVRSLLENSVNTFIPLSDVIENLKLYLELESLRFDQRFNYDIRTENLDIYAIEMPS
                     MLIQPYVENSIWHGLIKKEGEKNIQIKFYRNYNYLICEITDNGIGREKAAALNLKKQH
                     KSLGTIITKEMFETLHKIKETDYSVEIIDLYDEQHLPAGTKVWIRIELN"
     gene            13538..14299
                     /locus_tag="PLL59_04080"
     CDS             13538..14299
                     /locus_tag="PLL59_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMY23016.1"
                     /translation="MEQIKAIIVDDEKNNRIALHKLIEKFCPNVSIISECDSVDCAIS
                     TLEQTPPDVIFLDVEMPGRNGFDLLAHFNYQCPFEVIFTTAYSQYAVKAFRFSALDYL
                     LKPVDPEELIRAVHKLSQKISDETKNKQFELLEQKISNKDLGKKQLAISTLEGIYFAT
                     LDEIIHVDADSSYTKIYLTSGEMIMSSKPLKYFEELLEDYDFVRVHQSHIINLKLIRR
                     YVRGDGGQVIMLNGTEIEVSRRKKDDLLAKISETL"
     gene            14304..14828
                     /locus_tag="PLL59_04085"
     CDS             14304..14828
                     /locus_tag="PLL59_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY23017.1"
                     /translation="MFPILHTTRCQLRQFSDDDLENVFKGLSHPDIIKHYGVSYATLE
                     DTKAQLEFFADLEREETGIWWAICDIASNAFLGAGGFNNLSKTHRKAEIGFWLLTEHW
                     SKGLMTEVMPVICQYGFENLHLHRIEGFVESDNHLCKKAMDKLDFTLEGTMHDCEIKD
                     GNFISLDIYAKINS"
     gene            complement(14818..15864)
                     /locus_tag="PLL59_04090"
     CDS             complement(14818..15864)
                     /locus_tag="PLL59_04090"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HMY23018.1"
                     /translation="MYKIIRFFLFKLAAETAHHLTLHWLQFLIKLPFVKSIFKLLYAV
                     ENKKLERKIFGLTFKNPVGLAAGLDKNAECIDAFAALGFGFVEIGTVTPLAQPGNDKP
                     RLFRIIQDEAIINRMGFNNEGMSLVKENILKSAISNQQSAIIIGGNIGKNKITPNENA
                     TDDYVKCFHELYDVVDYFVVNVSSPNTPNLRALQEKEPLKQLLFTLQEINQQKIKPKP
                     ILLKIAPDLSKEQLDDIVEIVFETKLAGLVATNTTISRDNLSAEKSKIDSIGAGGLSG
                     KPLTKKSTEIIQYIAQKSENKIPIIAVGGIMTAQDAIDKLNAGASLVQLYTGFIYEGP
                     DLIKAINQQILAKN"
     gene            15968..17224
                     /locus_tag="PLL59_04095"
     CDS             15968..17224
                     /locus_tag="PLL59_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HMY23019.1"
                     /translation="MQKPLAERVRPQTLSQIIGQQHILGEGKPLRNLLEKQKLHSLIF
                     WGPPGVGKTTLARILASQAGLPFEQLSAINAGVKELRSVIDEAKTFGAKVLFIDEIHR
                     FNKSQQDALLGAVENGTIILIGATTENPSFEVNAALLSRSQVYVLKSLEAEDLKKIVD
                     TALQEDEILKQEKIIVKEYDALFYFSGGDARKLLNILELVCENLKADKTINNTLVEEI
                     IQTYIARYDKSGEQHYDIISAFIKSIRGSDPNAAVYWMARMIKAGEDPKFIARRLLIL
                     ASEDIGNANPNALLLANACFQAVTTLGYPECRITLAQCVTYLASSPKSNASYMAINDA
                     LALADKTSQLSVPLHLRNAPTKLMKNLNYGKDYQYAHHYENNFVNIEFLPAEISGTKF
                     YEPGINARENELRKFLKERWKEKYNY"
     gene            17261..17665
                     /locus_tag="PLL59_04100"
     CDS             17261..17665
                     /locus_tag="PLL59_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMY23020.1"
                     /translation="MTTQKGINDLAYKIIGCAIEVHRELGPGLLESIYQKCMEEELKN
                     RNLSFQSQINIPIKYKTITLDCDLRLDILVEDLIIIELKAVETLLPLFHAQLLTYMKL
                     LKKPKGLLINFNSDNIVNNTVSLVNQHFNEYL"
     gene            17706..18494
                     /gene="kdsA"
                     /locus_tag="PLL59_04105"
     CDS             17706..18494
                     /gene="kdsA"
                     /locus_tag="PLL59_04105"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HMY23021.1"
                     /translation="MVNLNTDKFFLIAGPCAVESKEICFQIAEELKAITTDLNIPFVF
                     KASYKKANRTKLDSFSSIGEEKALQILADVKSHFNIPILTDVHETADCEKIKDLVDII
                     QIPAFLCRQTELLLAAGKTGKAVNIKKGQFVSAEMMRFAAEKIASTDNKNIMLTERGT
                     MFGYNDLIVDFRNIPLMKQIGYPIVFDATHSVQQPNQSEGTSGGRPEFIETLAKAAIV
                     AGADGLFLETHTNPKEALSDGANMLALSEVASLLLKLKKIKQAL"
     gene            complement(18489..20369)
                     /locus_tag="PLL59_04110"
     CDS             complement(18489..20369)
                     /locus_tag="PLL59_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY23022.1"
                     /translation="MRKKLIILFSYFILLSTCNSFAQQVPQKAMAAYFKAKEYGSRTQ
                     WEEGIIELKNAIKIAPNYTQAKELLGEYYYALKKYEDAAIVLEDAANSADFSGRSTFL
                     TSEIFVKLNNGDKAKWYAEKYLARKDKAPNASIKAEQTIRNVNFAIEAKKNPVPFQPI
                     NLGANINTAQLEYFPYLTPDSKILAFTRLDGREENLYMSTKIDSAFSPAISFGDIINT
                     EENEGAETMNADGSLLFFTACNRPTGYGSCDIYFSQKLIKAWTPPNGIGKPINTSAWE
                     AQPSFSSDSKALYFSSNRAGGFGGKDIWVSYLDDNMKWSEPVNLGPNINTKNDDQCPF
                     IHADNQTLYFTSNGWPGMGNGDIFVSRKTDTSWTEAVNIGFPINTENDDNGLTVSYDG
                     KTAFLSSSREGGFGGLDIYSFELPENVQPQKITYIKAVIKDAKTNQMLNANYSIVDLD
                     SKMEEHKGSSSNGMFFVSIALNKNNALQIQKEGYLFYSQNINLKENTTQAKPYELLVL
                     LEPIATNNKITLNNVFFDFDKSDLKAESFLELDRLVELLKKNATIKIEIAGHTDNKGD
                     TKYNQTLSQKRAESVVNYLLQKGIDKTRIVAKGYGETQAISPNDTEENKAKNRRIEVR
                     VL"
     gene            complement(20430..21044)
                     /locus_tag="PLL59_04115"
     CDS             complement(20430..21044)
                     /locus_tag="PLL59_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HMY23023.1"
                     /translation="MQFNPATHIPVMEHFYTIQGEGFYTGHAAYFIRLAGCDVGCVWC
                     DVKASWTAEAHQLLQIDELVQEVLKYPGRLVVITGGEPLMYDLSYLTLALKENGFQIN
                     IETSGAYPISGSIDWICVSPKKFKAPLKTVLAAANELKCIIFNRSDFDFAEQNRKSVN
                     NDCKLYLQTEWSKKEQLLPLMIAYIKEHTEWNISTQTHKYLDIP"
     gene            21246..22613
                     /locus_tag="PLL59_04120"
     CDS             21246..22613
                     /locus_tag="PLL59_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HMY23024.1"
                     /translation="MLSEILQASSKKIIQNISDPFPYIPGDLKLPILGSSIELFLDAN
                     KLIVQKWEKYGNIFKIRVAGENMVVLIGAEANGLVLQDTAGNFSAKEGWNFIIGDLFK
                     GAILLSDGEEHARYRRIMQTAFHRDPMLGYIKVIEDTVQHYFDHELTIKNNKLITFPT
                     IDRLLVLIAGKLFFGFDFTTAQVAAIKSIVHASVTPLHLEIPFTPYWKGMQARRMLAK
                     FYKEKIKANRKNPSEDIFSQLCVAQSENGEMLSDDEIISQMVFMMMASHDTTTSTITS
                     MIYESAKSIEWQEKMRNESIAFYKEGPINYGRIKDLNNINKVFLECLRLHPALIILPR
                     MAIKEVTFEGHTIPAETRVVLSTYATHIDPKIYSNPLLFDPERFNEERAEHRKVTHSF
                     IPFGTGKHLCLGKYFAEIVAKIVISHFVKRFEWQVPKNYIMRYAPPLSHPKDGLPIFI
                     KRIDK"
     gene            complement(22754..24355)
                     /gene="pckA"
                     /locus_tag="PLL59_04125"
     CDS             complement(22754..24355)
                     /gene="pckA"
                     /locus_tag="PLL59_04125"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HMY23025.1"
                     /translation="MNDKGVKNPSADLTKLGFQHTTANWNSTRDELIQRTLDLNLGVL
                     NNTGALCINTGEFTGRSPKDKFTVKDALTENAVDWNNINQAYSTEAFDALQTKVVAHL
                     DAKSEIFVRDVSACADANYKVNVRVITETPWASLFADNMFIRPSDEEKQSIEPDWTIL
                     CAPSFMADAKSDATRQHNFSIINFTKKVILIGGSGYTGEIKKGIFTVLNFELPHLKNV
                     LSMHCSANIGNDGDTALFFGLSGTGKTTLSADPNRKLIGDDEHGWSNEGVFNFEGGCY
                     AKCVDLTEEKEPDIFRAIKHGAILENIGFYEGTNEVNFEDVKITENTRVSYPLFHINN
                     IAVPSVGTTPKNIFFLSYDAFGVLPPISKLTEAQAMYLFISGYTSKVAGTEAGVTEPQ
                     TTFSACFGAAFLPLHPTKYAEMLGNKLKESGANVWLINTGMTGGVYGVGKRMSLKYTR
                     ALITAALNGELNNAEFETLPIFNFQIPSAVEGVPAEILNPRNTWTDKAAYDAKAGELA
                     AKFNDNFKKYADQASAEILAAAPKV"
     gene            complement(24562..26442)
                     /gene="rpsA"
                     /locus_tag="PLL59_04130"
     CDS             complement(24562..26442)
                     /gene="rpsA"
                     /locus_tag="PLL59_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HMY23026.1"
                     /translation="MTDKPEIPAIDANEDIIPAHKEKEVIEREVVDIKIPVSKTEEFD
                     WNVSKRGANQYSKEKIAELETLYDQTFKQVEDNEIVKAIVTGITDADVILNIGFKSDG
                     MISKSEFKDTPDLKIGDEVDVYVENKENKKGQLILSRKKAKLTKAWDYICEANAKDIV
                     VKGRVISKTKGGLIVDLYGMETFLPGSQIDVKPITDYDQFVGKTMEFKVVKINETIKN
                     AVVSHKALIESDLESQRQEIIGKLEKGQVLEGTIKNITDFGAFIDLGGVDGLLYITDI
                     SWGRINHPNEVLKMDQKLQVVVIDYDDNKKRISLGLKQLQAHPWDSLPEGIEVGAKVK
                     GKIVNIEDYGSFLEIVPGVEGLIHVSEVTWSNQPINSRDFFKLGDEFEAVVVTLDKDE
                     RKMSLSLKQMQEDPWKHIAEKYPVGSKMKGLVKNITPYGVFVELDDVVGGMIHISDLS
                     WTKRYNHPSEFTKVGNELDVVVLEIDVENKKIVLGHKQLEEDPWDTFESLFPISSVHE
                     ATVLKREDKGAVVQLPYGIEAFAPTRHIKKEDGKLAEVEEVLPFKVLEFDRDDKRIIV
                     SHTRVNEDVKLEEKKSVDNEKKAAAKKTKDAVEKSNSKIEKSTLGDISALSDLKSKME
                     NE"
     gene            26846..>28380
                     /gene="purH"
                     /locus_tag="PLL59_04135"
     CDS             26846..>28380
                     /gene="purH"
                     /locus_tag="PLL59_04135"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HMY23027.1"
                     /translation="MKKIKSALISVYYKDGLDVLIHTLAKQGVVFYSTGGTQAFIENL
                     GYAVIPVEELTSYPSILGGRVKTLHPKVFGGILARRENADDIAQLKQYAIPELDLVVV
                     DLYPFEETVKNQSTLFAGELTSEIIEKIDIGGISLIRAAAKNFSDTVIVSQKNQYGIL
                     AQFLEENDGNTTLSFRQEFAKQAFAVSSHYDTLIHRYFSLQQANETFKESYLQGETLR
                     YGENPHQAGFYYGNINEVFDILNGKQLSYNNLVDIDAAVHLISEFTETTFAVLKHTNA
                     CGVASKASVLEAWKAAFAADTISAFGGVLITNASIDIATAEEIDKLFFEVLIAPDFSA
                     EALQILMKKKNRILLKQKLFNFPNKQFKSLLNGVIAQDFDKKIESSDELKTVTTIAPT
                     SQQVEDLLFANKIAKHLKSNTIVLAKDKQLIAMGCGQTSRVDALQQAIHKATTFGFSL
                     QGAVMASDAFFPFPDCVEIADKAGISAVIHPGGSIKDQDSIDYCNAHQMTMVLTGIRH
                     FKH"
BASE COUNT         9224 a         5066 c         4654 g         9436 t
ORIGIN      
        1 atgccaccca cttttgctgc tgctacaaaa acatattctg gtttgttggc ttcaaaccaa
       61 gcatttacat cagcttgatt tattaaattt aattctttag atgatgctgt tagaatattt
      121 gaaaaacctt cggctttcaa cttgcgaaca atggcagaac ctaccattcc attgtgtcct
      181 gcaacatata tttttgcatt tttttccata aaagtgataa ggcataaggc atatgtaata
      241 agtaaaaaat cttattactt attatttacc acttacgact attttattcg tgataattaa
      301 aaattttatg accaccttct aataaatatt tgtcacgttc aaatagttta atgtcgtgtt
      361 ccatcatatc ttttactaaa gaagctaaat cgtgttttgg ataccagcct agttttgtat
      421 ttgattttgt tggatcgccg attaataaat ctacttctgt aggtctgaaa taacgtggat
      481 cgactgccac tacttctgca ccaatttcta cttgatattt ttcgtttgag caagacttga
      541 tataaccttt ctcatcaatt ccagttcctt taaactctat ctcgatgcct acttctttga
      601 atgacatgcg tacaaactcg cgcacttctg ttgtttttcc agttgcaatt acataatctt
      661 ccggttcagc ttgttgtaaa attaaataca tcgcctccac ataatctttc gcatggcccc
      721 aatcgcgttt tgcatttagg tttcctaaat acaatttatc ttgtaagcct aaagcaattt
      781 tagaaacagc acgtgtaatt tttctagtta cgaatgtttc acctcgcaat ggtgattcgt
      841 ggttgaataa aataccattg catgcataaa ttccatacgc ttcgcggtag tttactgtaa
      901 tccaataacc atacattttt gcgactgcat atggtgaacg aggataaaat ggtgtttttt
      961 ccgactgtgg aatttcttgt actttgccat acaactcaga agttgatgct tgatataatt
     1021 tagttttatt ttcaagtttc aatattctga ttgcttccaa taaacgcaaa gaacctacag
     1081 catcaacatt ggcagtatat tcaggtgtgt caaatgatac tttaacatgt gacatcgcac
     1141 ctaaattata gatttcatca ggttgcactt gttgaataat gcgaattaaa tttgtagagt
     1201 cagacaaatc gccgtaatgc attataaaat tacgattatc tatatgtgga tcttgatata
     1261 aatggtcaat tctgtctgta ttaaataaag aagaacgacg tttgataccg tgtacttcgt
     1321 atcctttact taatagtaat tctgcaagat atgcgccatc ttgacctgta atacctgtaa
     1381 tgattgcttt tttcatgata atgtaaacat ctaaattaaa acgaaatgcg aaattacata
     1441 attttttcgt ttttttgagg ttaaatgaag ctaaaaattg cactattctt atccggctcc
     1501 ggcagtaatg ccagaaacat ctgttcctat ttcaaaaatc acgaacaaat tgaagtgtca
     1561 attatactca gcaataaaga aaattcaggc gcaaagcatg taagcgctga gtttgacata
     1621 ccttatttca tttttacgag agcagaattt tacgacacat caattgttgc ggaagaattg
     1681 aaaaaaaata aaattgatac acttgtcttg gctggttttt tatggctaat tcctagctat
     1741 ttactagagc aattccctaa tcaaatcatc aatattcatc cagcattgtt gccaaaatat
     1801 ggcgggaaag gtatgcacgg aaagcatgta cacgaggctg ttttttctaa taaagaaaaa
     1861 gaaagtggca ttactattca cttgtgcaat aaagagtatg acaaaggcga aattttattt
     1921 caagcaaaag tgagtttaac gaaagaagat aatccagaaa gtattgctgc aaaagtactg
     1981 caattagagc atcaacattt ccctttagtt atagaaaaat atatgcttgc taaataaaac
     2041 tgatatctac tcaataaaaa agccatacgc aaaacgtatg gctttataat aaaatgaaat
     2101 aaaattacag attgtatttt ccgcctgcta taattttagc tgcaactcta cgacgtgctt
     2161 cttttacgtt tactggttcg tatttagtaa aacgtttcaa gcccattaac ataatgcgaa
     2221 gctcatcgcc ttctgcaaat gattgtaatg catctttacc aaatacattg attttattca
     2281 ttgcatcgtt gaagaacact ttcaaaatat cgatatatac atcgtctgct ttatcgcctt
     2341 tttcatagat tttttgaaca cgcaacaaca atgattctgc tgcataaatt tgaatcaaca
     2401 tatcagacgt attcatgatg atttcttgtt cgtctttcaa ttttgccatc aatttttgaa
     2461 cagcgccacc agctactaat aagaacgctt ttttagcatt tttgatagct ttgaattctg
     2521 ctgcaaattg tccttcatcg tcgctgttga aatcaggaac actcatcaat tctttttgga
     2581 tagccatacc tggtgtcatc aaatcgattt taccagacat tgcacgtttt aacatcatat
     2641 cgatagacaa taaacgattg atttcgctag tgccttcgta gatacgtgcg atacgagaat
     2701 cacggtaagc acgagcagca tttgtttctt cagagaagcc cattccaccg tgtatttgaa
     2761 cgttttcatc aacgataaaa tctaacactt ctgagcctaa aactttaacg atagagcact
     2821 caattgcata ttcttctgca gcgcctaata atgctttaga gaattctttt ccgcttgcta
     2881 agttttcgtg ttctaatttt ccgatatcat ttgcacaacg ataagtggca acttctaaag
     2941 taaacaattg aatagccatt tctgctattt tatgttgaat agctccaaaa gtatttaatg
     3001 atttaccgaa ttgtgttctt tcgctcgcat attgtaccgc ttgattaaac gcttcacgtg
     3061 cgccacctaa tgctgttgca cctaatttat aacggccaat atttaagata ttaaaagcaa
     3121 ttaaatgacc tttacccctt tcgcctaaca tgttttcaac tggaattctg caattctcaa
     3181 aaaacacctg acgagtagat gaacctttga tgcccatttt gtcttcttct gcaccaagat
     3241 ttaaacctgg tgttcctgct tctactaaga aagcggtaaa ttctttacca tctatctttg
     3301 caaacacagt aaatgaatgt gcgaaaccag cattggtaat ccacattttc tggccgttta
     3361 aaatccattc tttaccatct tcagaaagcg ttgctattgt ttttgcacct aatgcatcag
     3421 agccagagct tggttctgtt aaacaataag aagcagccca ttcgcctgta gctaatttgg
     3481 gtaagaattt ttgtttctgc tcttcattgc cgaagtataa gattggcaat gtaccgatac
     3541 caatatgaca aatagatgtg gtagcaaaag aacctgttct tccaaattct tctgcaatga
     3601 tacaacctgt aattacatct tggttcatgc caccatacga ctcaggaatg gctgttgaca
     3661 acaagcctaa ttcgcctgct tttcttaata actccaaaga cagacctggt gtttgttttt
     3721 catattcttt agcacgtggc atcacatctt tttctacaaa gtcagaagcc atagagcgca
     3781 ccattaattg ctcttctgtg taatcttctg gcgtgaacac ttgttctgtg ctagattctt
     3841 tgatgatgaa ttcaccacct tttaatgatt taatttgttc gagtgtttcc attttaaata
     3901 ttaggtatta atttttgagt attggtatta agtaagctaa ttttcatatt aatctacaaa
     3961 atactaggta ctaaaattac acacaaacac agacatgtag gtaaaattgt tttttatgga
     4021 attgttagag atttcgatat tttaaaacca attaggcaat ggcttttatg atttgaatgc
     4081 tagctattta aaaagttttc attggcacat tcacttgttt ggcaaactta aaggcattat
     4141 taatggcaaa cattggatta aatactgaaa taaatgcttt ttcataccat ttataaccat
     4201 tttttgccat tttgatgcta gaaaaatgaa ataattcgcc gagtagcgag cctaatactg
     4261 gtgtgacaat tatgtcttgg atacttggtt gctcgattcc actttcaaga agatattccc
     4321 aaaccatgct ttgaccaaaa ctaaaaaggc ctgcttgcca aaattttgca ccttgcgagc
     4381 gcattgcatt atagtaataa gaaccttggt atggatgtcc tatgtaatta atataccaat
     4441 gatctttatc gacaactggt ttttgcgtaa atgccctttt ataattttgt ctataatttt
     4501 taatattctc tttgtcccaa ttacttacac ttgcgggaag cgcaaataag atgcccatag
     4561 tggtaccttg tatgcctaaa acgatgcctt ctgcccgtaa aaatttacgt ccaaaatttg
     4621 tcttttcatt tctaagaatt agcttgttat ttaaggtatc tcttttaggc ggaaaccatt
     4681 gccatttttt gttatttgaa attgattttt gtattgaatc tgtatgaggt aaaaataagg
     4741 aatcaacttg tgcaaatgaa agttgtgtgg atatacaaaa taatatcaaa aagtaagttt
     4801 tcatactcct acgttcaagt aataagtgtg gcaatattta aatagaaatt gaagcaaata
     4861 ttattccaaa tgagcagcaa caaaactaag aaagtcagta gcattttttt tcatcatttt
     4921 tatttagtaa ataatgagta ggttgtgtga attgtgtgga ataccttctt ttatttatac
     4981 ttaaattcca gctgccatgc tttgtgtcct cacaaaccat catcattaaa actcttttct
     5041 taaatccttt aaaaaatcaa attctttaat tgctgaaaca tgattcgtga ggacacgaag
     5101 catggctaag ggcatttttt ttcatcattt ttatttagta aataatgagt aggtgcatga
     5161 aagtaaattt caataatgaa gtaagtatat tataactcac taaaatttca aatttatttt
     5221 ctaatcaata aaaacaaaaa acgccaactt gctgttgacg tttttctgaa ataaaatgta
     5281 aatattttgg tgtttatttc aatctttcaa taatacctgc gatgccttgt ccgccgccga
     5341 tgcaggctga gaccataccg tattttttat tttgacggcg catttcatta attaattgaa
     5401 cagataattt tgcgccggag caacccagtg ggtgacctaa tgcaattgca ccgccatttg
     5461 gattgatgat atctggattt aaacctgcgg tgcgaattac ggctaatgat tgtgctgcaa
     5521 atgcctcatt caactctatc atttcgatat cgtctaattt caaaccagcc tgcttcaatg
     5581 cttgtggaat agccaccact ggaccgatgc ccataatacg tggctcacag cctactacag
     5641 cgcttgttac taaacgtgca attggttcga tgtttaattc tttcaccatt ttttctgaca
     5701 tgaccaacac gaatgctgca ccatcagatg tttgagatga gttacctgcg gttacgcttc
     5761 ctttagctgc aaacactgct tttaatgctg ccaaaccttc taccgttgtg tctttacgtg
     5821 gaccttcatc tgtgtcaacc acaaaagagc gtgtttttct tttattgttt tgcaagtaca
     5881 cttcttctac cgttacggga acaatttcat ctttgaattt tccactttcg atagcagcaa
     5941 tcgctttttg gtgtgagttg aaagaaaata aatcttgatc ttcacgagag ataccatatt
     6001 gttgtgcaac agcttctgct gttaagccca tagaagaata gtaatctggc gttgtagatg
     6061 ctatgccata gtttagtact gttctccagc ccgtcattgg caataaagac atagactcta
     6121 cgccaccagc aataatacac tctgccattc cagctttaat ttttgctgtt gccatagcaa
     6181 ttgtttcaag ccctgatgca caataacgat taacggtaac accgcctact gaaagtggca
     6241 aaccagcgcg caatacaatc atacgaccga tttgcatgcc ttgttcagcc tctggaatag
     6301 cattaccaca tattaaatca tcaatacgtg atggatctaa attaggtaca gatgctacaa
     6361 gatgtttaac tacatctgct gccaaatcat ctggacgata gaagcggaaa ccgccttttt
     6421 ttgctttgcc aatcgcactg cgataaccag ctacaatata tgcttcattc atttttttag
     6481 tattaagtag tgagtattaa gtattaagat ttttttattt taattaattt ataaatcatt
     6541 ctttgtattt cttgaatgtt tgtaataagt tctaaactac cttcttgttt tataaaatta
     6601 acatcaacgc ataataatag ttgagtttct aattcaaaac aagaaccata agccatttct
     6661 aaaaaataag caaactcttt ctttgaactg cgacttgcac cttcagcaat atttgaaggt
     6721 attgaaacta ctgatcttcg aatttgactt attaatccaa acttttcagc atttggtatt
     6781 tgttcagata acacataagt agctttaacc aatgtccttg atttttgcca aacaattaat
     6841 tctttaaaat tattcattct tactacttat gtcttataac ttacttctaa tttctcaatg
     6901 gtttaccagt ggtcaacatc gcttgaattc tttccaaagt tttcttctca ccacaaagtg
     6961 acaagaaggc ttctctttcc aaatccaata aatattgctc gtttactgtt gtttgtgctg
     7021 acaagtcgcc accacacata acataggcaa ttttcttagc gattttcgca tcatgtgcag
     7081 agatatatcc gccggcaaca aatgattcta cgccaacagt aagtgtacct aatcctgttc
     7141 ttccgagtac tgttatatcg ctacgatgtg caggttgtga gtagcctttt tcgtttaaac
     7201 gcaatacttc ttgttttgct tctgcaatca aacgttttcc gtttactact actctatctc
     7261 tgtttttacg catcaagcca aagtcgaatg cttcagcagc agatgtggct acttttgcca
     7321 ttgcaatatt caagaaatta tcttgtaatg tttgtaaaat tggatcacca tttttaatgg
     7381 aatcagatac tcttaaagtc aattcttttg taccaccacc tgcaggaatt aggcctacac
     7441 ctacttcaac taagccgata tatgtttctg ctgctgctac cacaacatct gagtgcattg
     7501 ttacttcaca gccaccacca agcgtcatgc catgaggagc tgaaactaca ggaatacctg
     7561 aatatctgca tcgcatagtc gtttgttgga attggcgtac agcgatatct aattcatcaa
     7621 attcttgttc cattgccaac atagaaatca tcatcaagtt agcgccaact gagaaattag
     7681 ttgcatcgtt tccgattaca agacctttgt atccgttttc tgcaatttca atagatttat
     7741 tgatagcttg taaaacatct ccacctaaag agttcatttt agatgtaaat tctaaattca
     7801 agacaccatc gccaatgtca tgcaaagtac caccactatt tttccaaact ggtttattgg
     7861 cacgtaaatt atctaagata ataaatgact cagcaccagg aattgtttta tatgattttg
     7921 attgttggtc gtagtatttt ttaacaccat tttcaacctt gtaaaaagag gtattgccag
     7981 cagcgatcat ttcttttacc caagcactaa tacttaagcc atcagcctct gccaaatcga
     8041 ttcctttttg aatgccaacg atgtcccaat attggaatgg gccgatttcc caagcaaagc
     8101 ctgctcgcaa agcatcatca acagcatata acacgtctga aatttctgga acacgattag
     8161 aaacataagc aaataaaccc gccaaagatc tttttactaa ttgcgctcct ttatcatccg
     8221 ctttgataaa tgcattaact ttatcagata aattttcaga ttttttagaa gcatctaaag
     8281 aagcaaattt gggtttgcca gtagcttcgt attgtaaagt atctaaacga agtgagaagg
     8341 aatttctttt ttcgtctttg tagtaataac cttttttcgt tttgttgccg aagaatttat
     8401 tatctaataa aaattgcaca taagcaggaa caacgaaggc attcgcttgt tcgtcgttag
     8461 ggcaattttc tttaataccc atcattactt ttgtgcctgt atcatttcct actaaatcag
     8521 acaatttaaa agttcctgtt tttggacgac caattgctgg acccgttagc gcatctactt
     8581 cttcaatagt taaacccaac tcttgtgtaa gctggaatac ttttgccatg gcatatacgc
     8641 caactctatt agcgataaaa gctggtgtat ctttacacaa tacagtatct tttcccaaga
     8701 aaacatcgcc ataatgcatt aagaaatcca cgacattttg atctgttgtt ggtgttggaa
     8761 taacttctaa caaacgcatg taacgtggtg gattaaagaa gtgcgttcca cagaaattct
     8821 tttgaaaatc ttcgcttctg ccttccgcca ttaaatgaat tggaatacca gacgtattag
     8881 aagttaccaa tgaacctttt ttacgcaagg catctacttt ttcgaatact tgttttttaa
     8941 tatctaagcg ctcgattact acttcgataa tccaatcaca gtctttaatt ttaggcatgt
     9001 cgtcttcaaa atttccgagt gatactcttt ttgctgactc cgaattgtaa gcttgtgcag
     9061 gtttagattt taatgcaaat gtaaaagcat catttacaat tttgttgcgc atttttggat
     9121 tcgctttatc ttcttctttt aaatcaaaag gtacgatatc tagcaaaagc acttcgatgc
     9181 caatattggc aaaatgcaat gcaataccag aacccatgac accagaaccc aatacggcta
     9241 cttttttaat tactctgttt gacatactaa taaatttctt tatataaaat tagcaaaagc
     9301 actgctaaaa ttcgtcacat taaccttttg taatgataac ttaatatttc caatgcatgc
     9361 attggaaata tagggcaatg taaattattg aaattgaaca tcaaaatata aaaggtaaaa
     9421 ggcatgccta ttttagctca gctaaggtaa acgctgcaaa gcgattgact agccgttcat
     9481 aagcattttt tatatatttt gtatctttga aaatacgaga catcatcact gctccttcaa
     9541 actctgcaac tgtctgctct gctaatttca ttgcatcgtc ttcattcgtt atttctttaa
     9601 aaacaaacgc gacagcattt atccattctg tatagaacgc gcgaatatgt tccgcaaatt
     9661 ctggtatggc acgagctgtt tccacaccaa tatttcccat aatgtttcct tctgacaaga
     9721 gtgcgcgctc cgacttttta aacatctttt ccatgcgttg ttgtggcgac attgtcacat
     9781 cataagcaat tgaaaaaact tccttttgaa aatattcgtg agcatattta atcacggcac
     9841 tcatcaatgc ttccttagat ggaaaatagt gatataaact tccttttagt aagccacaag
     9901 cagcagcaat gtctgcaata gatgtattgt gataactttt ttgtcggaat aattttaaag
     9961 aatgctttaa aatatcatcc aagcttactt tctgtttagg cattacttga tgtattgtgt
    10021 ttaaagataa gtagtttcag taatttatca aacgtttgtt tggtaaataa tgaaaaaata
    10081 atagtatgtg atagcaatga aagtaaatct tgctgcgcta agcaattaaa ttagaccagc
    10141 taaaatgcca aattctcatt tatatatggt aaaataagcc aattattgac catgtataaa
    10201 acctaaacca agataaattg aaataagaat aactttaatt tattaaaata aaatactgca
    10261 acagccatca aaaagttgac gcagtaaatt agtgggtttt tatgtttcta tatttctttg
    10321 gggtgcattg cacctcattt tttttataaa aagaaacata tttcttaatt tttctatatt
    10381 ttgtaaaggt gtgcaactta tataaacgaa tacttctatc gagtttttta ctaataacaa
    10441 atgtagtact ccatattaca gtgtttggat tagatgtaac acaaattcgt tttgatgtta
    10501 tcgacattaa gcaaggactt tcgcaaaata gtgtttcaca tatttatcaa gatagcaaag
    10561 gctatatgtg gttttgtact gatgaaggct taaacagata tgatggttat agttttttga
    10621 aattggaaag aattataggc aatgaaaatt cattgatttc taattttacg acatgcataa
    10681 cagagtcaaa acctggcata ttttggattg gcacagaaac aggattaagc gtttatttgt
    10741 ttgatgagaa gaaatttttg catgtagaaa atcgtggtca aatcacaaaa atatttgtcg
    10801 aagacagttt ctccgtttgg atacaaacac aatacagttt gtataaaata aggttagtag
    10861 caaacaataa aagtgccaat gattacactt taaacattga gccattgaat gctttaaaat
    10921 cggttatctg caagctcgat gtcaatcatt ttttgattgc cggcagagac aataagctat
    10981 acctatatga cataagaact aagcttttgc aaaataaaag caagtatgat atatggaaaa
    11041 ttattattcc taaacatatc aacgatgtta taaaagatag ggaaaataat ttttgggtgg
    11101 ccacaaacgc tggattatat aaagtcaaca gccaacaaaa cagtatcgac tcattttcag
    11161 aaaacagaaa aagtattcat ggtttaaacg aaagaataac agatgttgca gttggtaaaa
    11221 tgggcaatat ttatattgca accgccaaaa atggactagt catttatgaa gcagagaaaa
    11281 ataaatttaa cgaataccag agcgaccctt acaatactac tagtttgcct gataataaaa
    11341 ttttgagttt attgacagac aatagcaata ccttatgggt tggcacgaaa ggtgctggta
    11401 tagccacgtt ttctcagttt aaatataaat tcaaacacat tacacaagaa atatttaaaa
    11461 caacttggct aaccaataaa tacatattga gtttagttgc tgataaaaat gaaaacattt
    11521 ggataggtac agatggtggc ggtctgttta aatttaatgc aaaagaaaat attttttcta
    11581 attggagata caatatatta gcatcatcac ttactgatga tgtagtacaa gaagttcttt
    11641 ttgatgtgta tgataattta tgggtaaata cattaaatgg aatctgtaaa tacaatccaa
    11701 acaacaataa atttactcga tattcattac cttatacgaa agcaaaagaa agcatttcaa
    11761 aagtatcgac ttattcgaat gtgctattat tttcatcaaa atcaggcgat ttttatgcat
    11821 tagacgagca gacaattttt aaattcaatt atttacttaa tcaatttgaa gtgctgccca
    11881 ttcctgtagc taatcagaat tttattccta gaaaaataat tgaagatatt gataacacct
    11941 ggtggcttgc tacatcaatt ggcatcatac acattgattt gaatacagga atattacaga
    12001 aagatgcatt aaatgcaatc aataataaat tttttaagaa cgaccaactg tattgtttaa
    12061 tgcaacatga cgaagataga atttggattg gctcaggcaa taatggctta tttcttttta
    12121 ataaaaaaaa gatgcagata gaagcaaact atacggaaaa agatggcatg agcaacaatt
    12181 ttgtatatgg cattttgaaa gataaagttg gaaaaatatg gatgagtact aacagtggaa
    12241 ttacggtatt cgatcctata cttaaacaat ttagaacctt cgatatcaat gatggtttgc
    12301 agagcaatga atttaataaa ggcgcatatt acaaaacagc aaataatgaa ttattatttg
    12361 gcggaataaa cggctttaac attatttcgc caacaaatat tccatttaat aactacaaac
    12421 cacttgccaa tattactggt ataaaagtaa acgacagctt gctcctgcca cttgcttaca
    12481 gcaatcaatc aaaacgcgta cgtttcgcaa acagtcaaaa taatatcaca tttgagtttg
    12541 catcgagcga ctttgccaac acaccaaaaa atgtatttga atacaaatta gaaggatacg
    12601 aaaaaaattg gacgcgcaca agacgcaatc atgctacata cacaaaacta tcaccaggga
    12661 attatacttt ctacgtacgt gtaagcaaca atgacggtct ttggtcagca cagccagcag
    12721 catactcatt tagagtgcgg ccattaattt ggcaaacttg gtggtttaaa ataattgcag
    12781 gaattttatt tctcctttat gcttatcaac tcgttagcaa tagaattcaa gcagcaagaa
    12841 aaaaagaaaa agaaaaaaca caattagcac agcaaaaagc agagtttgaa aagcaattag
    12901 cagaaataaa attgaaatca ttagtggcgc aaatgaatcc acattttatt tttaattgta
    12961 tgaattccat tcaagcgatg attttatcag accaaaacat gcaagcatca acttatctaa
    13021 ctaagttgtc tagattagtg cgttctttat tggaaaattc tgtcaacacc tttattcctt
    13081 taagtgatgt catcgaaaat ttaaaactct atttagagtt agaatcattg cgctttgacc
    13141 aacgttttaa ctacgatatt cgcacagaaa atttagatat atatgccatc gaaatgccat
    13201 ctatgttgat tcaaccttat gtagaaaatt ccatttggca tggtctaata aaaaaagaag
    13261 gtgagaaaaa tatacagatt aaattttaca ggaattataa ttatctgatt tgtgaaatca
    13321 ctgacaatgg cattggtaga gaaaaagcgg cagcattaaa ccttaaaaaa cagcataaat
    13381 ctttaggcac tattatcact aaagagatgt tcgaaacatt gcataaaata aaagaaacag
    13441 attatagtgt agaaatcata gatttgtacg acgaacagca tctgcctgct ggcacaaaag
    13501 tatggatacg cattgaactt aattaaaacc tcatcacatg gaacaaataa aagcaattat
    13561 agtcgatgac gaaaaaaaca atcgcattgc actgcacaaa ttgatagaaa aattttgtcc
    13621 gaatgttagc attatatctg agtgcgatag tgtggactgc gctatttcaa ccctagagca
    13681 aacgccacct gatgtgatat ttttagatgt cgaaatgcct ggtagaaatg gtttcgattt
    13741 attagcacac ttcaattacc aatgtccgtt tgaggtaata tttactactg cttacagtca
    13801 atatgccgtc aaagcctttc gctttagtgc attagattat ttgctcaaac ctgtcgatcc
    13861 agaagagctc attcgtgccg ttcataagct gtctcaaaaa atttctgatg aaacaaaaaa
    13921 taaacaattt gaattattag aacaaaaaat aagcaataag gatttaggca aaaagcaatt
    13981 agcgatttct actttagaag gcatttattt tgcgacttta gacgaaatca ttcatgttga
    14041 tgcggattct tcttacacca aaatatactt aacaagtggt gaaatgatta tgtcttcaaa
    14101 accgctaaaa tattttgaag aattgttaga agattatgat tttgtacgag tgcatcaatc
    14161 acatattatc aacctaaaat taattagacg atatgtgcgt ggtgatggcg gacaagtcat
    14221 tatgctcaat ggcacagaaa tagaagtgtc tcgcagaaag aaagatgatt tattagcaaa
    14281 aatttcagaa actttataac attatgtttc caatattaca tacaacacgc tgtcaactta
    14341 gacaattttc agatgatgat ttagaaaatg ttttcaaagg actttcacat ccagacatca
    14401 tcaaacatta cggtgttagc tatgctacac tcgaagacac aaaagcgcag ctcgaatttt
    14461 ttgcagattt agaaagagaa gaaacaggca tttggtgggc catctgtgac atagcaagta
    14521 atgcgttttt aggtgccggt ggtttcaata atcttagcaa aacgcatcga aaagctgaaa
    14581 tcggattttg gttattaaca gaacattgga gtaaaggatt aatgacagaa gtaatgcctg
    14641 ttatttgtca atatggattt gaaaatctac acttacatag aattgaagga tttgtagaat
    14701 cagacaatca tttatgcaaa aaggcaatgg ataaattgga ctttacattg gaaggcacca
    14761 tgcatgattg cgagataaag gatggcaatt ttatcagctt agatatttat gcaaaaatta
    14821 attcttagct aaaatctgtt gattaattgc ttttatcaaa tcaggtcctt cgtaaataaa
    14881 accagtatat aattgcacta aacttgcacc agcatttaat ttgtcgattg catcttgcgc
    14941 cgtcatgatg ccgccaacag caataatcgg aattttattt tctgattttt gagcaatata
    15001 ttgtatgatt tctgttgatt tttttgttaa aggttttcca ctcaaaccac ctgcaccaat
    15061 cgaatctatt ttactttttt ctgcgcttaa attatcgcgt gaaatggtgg tattagttgc
    15121 tacaagtcca gctagttttg tttcaaaaac aatctccaca atatcgtcta attgttcctt
    15181 acttaaatca ggagctattt ttaataaaat gggtttgggt tttatttttt gctgattgat
    15241 ttcttgcaaa gtaaatagta attgcttcaa tggctctttc tcttgcagtg cccgtaaatt
    15301 cggtgtgttt ggcgagctta cattcaccac aaaataatcg actacatcat ataattcgtg
    15361 gaaacatttt acataatcat cagtagcatt ttcattcggt gtaattttat ttttgccaat
    15421 attgccacca atgatgattg ctgattgctg attgctgatt gctgatttta agatattttc
    15481 tttaacaagg ctcatgcctt cgttattaaa gcccattcta ttgatgatgg cttcatcttg
    15541 aatgatgcga aacaaacgag gtttgtcatt tccgggctgt gctaatggcg ttaccgttcc
    15601 tatttcaaca aagccaaaac ctaaagcggc aaatgcatca atacattctg catttttatc
    15661 caagccagcc gccaaaccaa ctggattttt aaatgtgagt ccaaagattt tacgttctaa
    15721 ctttttattt tctacagcat ataataactt aaaaatagat tttacaaaag gtaactttat
    15781 caaaaactgg agccagtgta aagtaagatg atgcgccgtt tcagcagcta atttgaataa
    15841 gaagaaacga ataattttat acatagtgta aaaataacac taaaagcata aatgatataa
    15901 aatcaaatgg taaattatac atcatttgtc cattagctaa tcagctaatt tattttatct
    15961 ttactctatg caaaagccac ttgccgaacg cgttcgacct cagactttat ctcaaataat
    16021 tgggcaacaa catattcttg gtgaaggcaa acctttgcgc aacttattgg aaaaacagaa
    16081 gcttcattcg ctcatttttt ggggtccgcc tggtgttgga aaaacgactt tagcaagaat
    16141 tttagcatca caagccgggc ttccttttga acaattgagt gccatcaatg ctggcgtaaa
    16201 agaattgcgt agtgtgattg atgaagcaaa aacttttgga gccaaagttc tatttattga
    16261 tgagattcat cgctttaata aatcacaaca agatgcgctt ttaggagcag tagaaaatgg
    16321 caccattatt ttaatcggcg ccacaactga aaatccatct tttgaagtaa atgctgcatt
    16381 gttatcgcgc tcacaagtat atgtattgaa atccttagaa gcagaagatt tgaaaaaaat
    16441 agtagatacc gctttacaag aagatgagat tctaaaacag gaaaaaatta ttgtcaaaga
    16501 atacgacgcg ctcttttatt tttcaggtgg cgatgcacga aaattgttga atattttaga
    16561 attagtatgc gaaaatttga aagcggataa aacgataaac aatactcttg ttgaagaaat
    16621 tatccaaacc tatattgccc gatatgataa aagtggcgaa cagcactacg atatcatttc
    16681 tgcatttata aaatctatca gaggtagcga tccaaatgcc gctgtgtatt ggatggcacg
    16741 catgatcaaa gctggcgaag atccaaaatt tattgcacgg cgcctgctta ttttggcaag
    16801 tgaagatatt ggcaatgcca atcctaatgc gcttttgctg gccaatgcct gctttcaagc
    16861 agtcactacg ttgggatatc ctgaatgcag aattactttg gcgcaatgcg ttacgtattt
    16921 agcatcttca ccaaaaagca atgcgagtta tatggccatt aatgatgcat tagcattggc
    16981 agataaaaca agtcagcttt ctgtgccttt gcatttgcgc aatgcaccga ctaagttgat
    17041 gaagaattta aactatggta aagattacca atatgcacat cattatgaaa ataatttcgt
    17101 gaatatagag tttttgccag cagaaattag tggtacaaaa ttttatgaac caggaataaa
    17161 tgcgagagaa aatgaattga gaaaatttct gaaagaaagg tggaaagaaa agtataatta
    17221 ttgattttaa ccacttaagg aacttaagaa aataagaata atgacaacac agaaaggcat
    17281 aaatgattta gcatataaaa ttattggatg tgccattgaa gtacatagag aattaggacc
    17341 tggtttgctc gaatctattt atcaaaaatg tatggaagaa gaactgaaga ataggaattt
    17401 atcctttcag tctcagataa atattcctat aaaatataaa actattacat tagattgtga
    17461 cttaagatta gacatcttgg ttgaagattt aataattatt gaattaaaag cagtagaaac
    17521 tttgcttcct ttatttcacg cacaattgct aacttatatg aagctattaa agaaacccaa
    17581 aggtttatta attaacttta attcagacaa tatagtaaat aatactgttt ccttagtaaa
    17641 tcagcatttt aatgaatatc tttaatcttt gtttctttag tttctaatgt ggtaaattaa
    17701 aataagtggt aaatctcaat actgataagt tcttcttaat tgctggacct tgtgcagtag
    17761 aaagcaaaga aatttgtttt caaatagcag aagaattaaa agcaataaca actgacttaa
    17821 atattccatt tgttttcaag gcatcataca aaaaagccaa tagaaccaaa ttggattctt
    17881 tttctagtat tggtgaagaa aaagctttgc aaatattagc agatgtaaaa tcgcatttca
    17941 atattcctat tcttacagat gtgcacgaaa cagcagactg tgaaaaaata aaagaccttg
    18001 tagatatcat acaaattcca gcatttctat gtcggcaaac agaattatta ctcgcggcag
    18061 gaaaaactgg caaagccgta aacatcaaaa aaggacaatt tgtttccgct gagatgatgc
    18121 gatttgcggc agaaaaaata gcctctacag acaataaaaa catcatgttg acagagcgag
    18181 gcacaatgtt cggctacaac gatttgattg tcgattttcg caatatacct ttgatgaagc
    18241 aaataggata tcctatcgta tttgatgcaa cgcattccgt acaacaaccc aaccaaagtg
    18301 aaggaacaag tggcggaaga cccgaattta ttgaaacatt ggcaaaggcg gctattgttg
    18361 caggtgctga tggtctcttt ttagaaacac atacaaatcc gaaagaagca ctaagtgatg
    18421 gcgcaaatat gttggcattg agtgaagtag catctcttct attaaagctt aaaaaaataa
    18481 aacaagcatt ataaaaccct cacctctatt cttctgtttt tagctttatt ttcttctgtg
    18541 tcatttggtg atattgcttg cgtttcacca tagccttttg ccacaattct tgttttgtct
    18601 attccttttt gcaataggta attgaccacg ctttctgcgc gtttttgtga taatgtttga
    18661 ttgtattttg tgtcgccttt gttgtctgta tgtccagcaa tttcaatttt tattgtcgca
    18721 ttcttcttta ataattcaac caatctatcc aattctaaaa aagactctgc tttcaaatca
    18781 cttttatcaa aatcaaagaa aacattgttc aaagtaattt tattattagt agcaattggt
    18841 tcaagtagca ccaacaattc ataaggcttt gcctgtgttg tattttcttt taaattgata
    18901 ttttgcgaat aaaatagata accctctttt tgaatttgca atgcattatt tttatttaat
    18961 gcaatagata caaaaaacat tccattcgaa ctcgaacctt tgtgctcttc catttttgaa
    19021 tctaaatcaa caatagagta attggcattc aacatttgat tagttttagc atctttgata
    19081 acagctttga tataggtaat tttttgaggt tgcacatttt ctggcaattc aaaactataa
    19141 atatctaagc caccaaaccc gccttctcga ctagaagaca gaaatgctgt cttgccatcg
    19201 taggaaacgg tcaaaccgtt gtcgtcattt tccgtgttga ttggaaagcc aatatttaca
    19261 gcttctgtcc aagaggtatc tgttttgcgc gacacaaaaa tatcgccgtt gcccatgcct
    19321 ggccagccat tagatgtaaa atataaagtt tgattatcag catgaataaa tggacattgg
    19381 tcgtcgtttt tggtattgat atttggccct aaattcacag gttcactcca tttcatgtta
    19441 tcgtctaaat aacttaccca aatatctttt ccgccaaatc cgcccgctcg gttgctcgaa
    19501 aaatacagtg ctttactatc tgatgaaaaa cttggctgtg cttcccaagc acttgtattt
    19561 ataggctttc caatgccatt tggtggcgtc catgctttga ttaatttttg ggagaaatag
    19621 atatcacaac tgccatagcc tgtaggccta ttgcatgcgg taaaaaataa taggctacca
    19681 tcagcattca tcgtttcagc tccttcgttt tcttctgtgt taatgatatc gccaaatgaa
    19741 attgcaggag aaaaggcaga atcaattttt gtggacatat acaaattttc ttctctgcca
    19801 tctaatcgtg taaatgctaa aattttactg tctggtgtta agtatggaaa atattctaat
    19861 tgtgctgtat taatatttgc tcctaaattg ataggttgaa atggaacagg atttttttta
    19921 gcttcaatgg caaaattcac attgcgaata gtttgctcag cttttatact tgcattgggt
    19981 gctttatcct ttctggctaa atatttttca gcataccatt tcgctttatc gccattattc
    20041 aattttacaa aaatttctga ggttaaaaat gtggaacgtc ctgaaaaatc ggcggaattg
    20101 gcggcatctt ctaagacgat ggcagcatct tcatattttt ttaatgcgta ataatattcg
    20161 cctaaaagtt ctttggcttg tgtgtaattt ggcgctattt ttatagcatt ttttaattca
    20221 attatgcctt cttcccattg tgtgcggctt ccatattctt ttgctttaaa ataagcagcc
    20281 attgcctttt gtggaacttg ctgtgcgaaa gaattacaag tagaaagtaa tatgaagtaa
    20341 gagaataata ttatcagttt ctttctcatt aaattgaatt aatcaaaata tataccattc
    20401 tttttattta ttacatatca ctattactgt taaggaatat ccaaatactt atgtgtttgt
    20461 gtagaaatat tccattcagt atgttctttt atgtaggcta tcattaatgg caataattgt
    20521 tcttttttcg accactctgt ttgcaaatac aatttacagt cattgtttac cgattttcta
    20581 ttttgttcag caaagtcgaa atcgcttcga ttaaagataa tgcattttaa ttcatttgca
    20641 gctgctaata ctgtttttaa gggtgctttg aacttttttg gcgacacaca aatccaatca
    20701 atacttccac taattggata cgcaccagac gtttcgatat tgatttgaaa tccattttct
    20761 tttaatgcca atgttagata agataaatcg tacatcaatg gctcaccacc tgtaatcacg
    20821 actaatcgcc ctggatattt taatacttct tgcactaatt catctatctg caataactga
    20881 tgtgcttctg ctgtccacga tgcttttaca tcacaccata cacaaccaac gtcacagcca
    20941 gctaatcgta tgaaataggc agcatgacca gtgtaaaatc cttcaccttg tatggtgtag
    21001 aaatgttcca ttactggaat gtgtgtagct ggattaaatt gcatggcact tcaaaaataa
    21061 aaaaagattc ccgctttatg ctatcctttc tattttgttt gatttgcata ttatattaca
    21121 ataacacgac ctgatttttt tcattgcatt aaacaagaaa aagaaaatct tgctcattta
    21181 tgctccaata aaattgagaa attaaaaata attgtaactt caattttgca ataaatcaca
    21241 acttcatgct gtcagaaatt ttacaagcaa gtagcaagaa aattatacaa aatatcagcg
    21301 atccatttcc atatattcct ggagatttga aacttcccat attaggttcg agtatagaat
    21361 tatttttaga tgccaataaa ttgatagtac aaaagtggga gaaatatgga aatatcttta
    21421 aaatcagagt agctggagaa aacatggtag tgctcattgg tgcagaagca aatgggcttg
    21481 ttctacaaga cactgccgga aatttttctg ctaaagaagg ttggaatttt attattggtg
    21541 atttattcaa aggtgccatt ttattaagcg atggcgaaga acacgcccgt tacaggcgca
    21601 tcatgcaaac tgcttttcat cgcgatccga tgctaggtta tatcaaagta attgaggaca
    21661 cagttcagca ttactttgac catgaattaa ctataaaaaa caataaactc attacctttc
    21721 caactatcga taggctttta gtgctaatag ctggaaaatt attttttggt ttcgacttta
    21781 ctacagcaca agttgctgcg ataaaatcaa ttgtacacgc cagcgttaca cctttacatc
    21841 tcgaaatacc atttacgcca tattggaaag gcatgcaagc acgacgtatg ctagctaaat
    21901 tctataaaga aaaaataaaa gcaaacagaa aaaatcctag cgaagatatt ttttctcagc
    21961 tgtgtgtggc tcaaagtgaa aatggcgaaa tgctctctga tgatgaaatc attagtcaaa
    22021 tggtatttat gatgatggca tcacatgata cgacaacgag caccattacg agcatgattt
    22081 atgaaagtgc aaaatccata gaatggcaag aaaaaatgcg caatgagagt attgcatttt
    22141 acaaagaagg gcctattaat tatggcagaa taaaagattt aaacaatatt aataaagtat
    22201 ttttagagtg cctgcgcctg catcctgctt taataatatt accacgtatg gccataaaag
    22261 aggtgacgtt tgaaggacat acaattcctg cggagacgcg tgttgtgctg tccacctatg
    22321 caacacacat cgatccaaaa atatatagca atcctttgct atttgaccct gagcgattta
    22381 atgaagaacg tgcagagcat aggaaagtaa cacatagctt tattccattt ggtacaggta
    22441 agcatctgtg tttagggaaa tattttgctg aaattgttgc taaaatagta atctctcatt
    22501 ttgtaaaaag atttgaatgg caagtgccta aaaactatat aatgcgatac gcaccacctt
    22561 taagtcatcc gaaagatggc ttgcctattt tcatcaaacg aatagataaa taactcggaa
    22621 aaatattaca cattttttcg ggcaaacgac atgatgaaat caagcatttc aacatatcga
    22681 ataacttgtt attttataaa aaaaagatgc acaatttctc gtgcatcttt cctatttaaa
    22741 caattaatat tttctatact tttggtgcag ctgctaaaat ttcagcactt gcttgatcag
    22801 catatttttt aaaattatca ttgaattttg ctgccaactc tcctgctttc gcatcataag
    22861 cagctttatc tgtccaagta tttctcggat ttaaaatttc tgctggcacg ccttcaacag
    22921 cgcttggaat ttggaaattg aaaataggta atgtttcaaa ttcagcatta tttaattcgc
    22981 catttaatgc tgcagtaatt aaagcacgcg tatatttcaa actcatacgc ttaccaacac
    23041 catacacacc accagtcata ccagtattaa tcaaccaaac attggctcca ctttctttta
    23101 gtttattgcc cagcatctct gcatattttg ttggatgcaa tggtaaaaat gcagcaccaa
    23161 aacaagctga gaatgtagtt tgcggctccg taacaccagc ttcagttcca gctacttttg
    23221 aagtgtatcc agaaataaat aaatacatgg cttgcgcttc tgttaattta gatattggag
    23281 gcaatacacc aaatgcatca tagcttaaaa agaatatgtt ttttggcgta gtaccgacag
    23341 aaggaactgc aatattattg atatgaaata aaggatagga aacacgcgta ttctctgtga
    23401 tttttacatc ttcaaagttt acttcatttg taccttcata gaatccaata ttttctaaga
    23461 tagcaccatg tttaatggca cggaagatat cgggttcttt ttcttcagtt aaatctacac
    23521 attttgcata gcaacctcct tcaaaattaa atacgccttc attactccaa ccatgctcat
    23581 catcgccaat caatttacga tttgggtcag cagaaagtgt tgtttttcct gtgcctgaca
    23641 agccaaagaa taatgctgta tcaccatcat taccaatatt tgcggagcag tgcatgctca
    23701 acacattttt tagatgtggt aattcaaaat ttaatacggt aaaaattcct tttttaattt
    23761 cgcctgtgta gccgctacca ccaattaaga ttactttttt agtaaagttg ataatcgaaa
    23821 aattatgttg tcttgtagcg tcagactttg catcggccat aaaacttggt gcacataaaa
    23881 ttgtccaatc tggttcgatg ctttgttttt cctcatcact cgggcgaata aacatattat
    23941 ctgcaaataa gcttgcccaa ggtgtttctg taataacacg aacattaact ttataatttg
    24001 catcagcgca agcgctaaca tctctaacaa aaatttcaga tttagcatca agatgtgcta
    24061 ctacttttgt ttgtaaagca tcaaaagctt ctgtagaata ggcttgatta atgttattcc
    24121 aatcaacagc attttctgtc aatgcatctt taacggtaaa tttgtcttta ggagaacgac
    24181 ctgtaaattc gcccgtattg atacaaagtg cgccagtgtt gtttaaaaca cctaggttta
    24241 aatctaaagt acgttggata agttcatctc tggttgaatt ccaattggcc gttgtgtgtt
    24301 ggaaaccaag ctttgtcaag tctgctgaag ggttcttcac gcctttatca ttcatttttt
    24361 tgcgttttga gttaggattc caaaaataag actttttggt tttaaaaagc cgtcttaaag
    24421 ttttagtaac gttaaaagta atacacaatt tacgcttgta tattgataat taaagactcc
    24481 taatttttta tctgttatga ttatattaca aaaaaaagag cctgctcaaa taagcaggct
    24541 cttagtgaat ttaaaatgaa attattcgtt ttccattttt gattttaaat cagaaagtgc
    24601 actaatatcg cctaatgtag atttctcgat ttttgagttc gatttttcta cagcgtcttt
    24661 agttttttta gctgctgctt ttttctcatt gtcaacagat ttcttttctt ccaatttcac
    24721 atcttcgttt acacgtgtat gtgaaacgat gatacgtttg tcatcgcggt cgaattctaa
    24781 cactttaaat ggtaatactt cttctacttc tgcaagcttg ccatcttctt ttttgatatg
    24841 acgagttggt gcaaacgctt cgataccata aggcaactga acaacagcac ctttgtcttc
    24901 gcgttttaaa acagtagctt catgtacaga actgattggg aacaatgatt cgaatgtatc
    24961 ccatggatct tcttctaatt gtttatgtcc taaaacaatt tttttgtttt ctacgtcgat
    25021 ttctaaaaca acaacatcca attcatttcc aacttttgta aactcagatg gatggttgta
    25081 acgttttgtc caagataaat cagagatatg aatcatgcca ccaacaacat catctaactc
    25141 aacaaataca ccgtaaggcg tgatgttttt caccaatcct ttcattttgc tacctactgg
    25201 atatttttct gcgatgtgtt tccaaggatc ttcttgcatt tgttttaaac tcaatgacat
    25261 tttgcgttca tctttgtcta atgttacaac aactgcttcg aactcatcac ctaatttgaa
    25321 gaaatcacga gagttgattg gttgattgct ccaagttact tcagaaacgt ggattaaacc
    25381 ttcaacgcct ggaacgattt ctaagaaaga accgtaatct tcgatattaa cgattttacc
    25441 ttttacttta gcaccaactt cgataccttc tggcaatgaa tcccaaggat gagcttgtaa
    25501 ttgttttaaa cctaaagaga tacgtttttt attgtcatca tagtcaatta ctacaacttg
    25561 tagtttttga tccattttca atacttcatt tggatggttg atacgacccc aagaaatatc
    25621 agtgatatac aacaaaccat caacaccacc taagtcgatg aatgcaccga aatcagtaat
    25681 atttttgatt gtaccttcta atacttgtcc tttttctaat ttgccaatga tttcttgacg
    25741 ttgtgattct aaatcgcttt cgattaaagc tttgtgagaa acaacggcat ttttgatagt
    25801 ctcgttgatt ttcaccactt tgaattccat tgttttacca acaaattggt cgtaatctgt
    25861 gataggttta acatcaattt gagaacctgg taagaatgtt tccattccat ataaatcaac
    25921 gattaagccg ccttttgttt tgctgataac acgtcctttt acaacgatat ctttagcatt
    25981 tgcttcgcag atataatccc aagcttttgt caattttgct tttttgcgag aaaggataag
    26041 ttgacctttt ttgttttctt tgttttctac ataaacatct acttcatctc caattttcaa
    26101 atcaggtgta tctttaaact ctgattttga aatcatacca tcagatttga aaccgatatt
    26161 taagataaca tctgcatctg taatacctgt aacaattgct tttacaatct cgttgtcttc
    26221 aacttgtttg aaagtttgat cataaagtgt ttctaactca gcaatttttt cttttgaata
    26281 ctggttagca cctcttttgc tcacgttcca atcgaactct tctgtttttg aaactgggat
    26341 tttgatatcc acaacttcac gttcgattac ttctttttct ttgtgtgccg gaatgatgtc
    26401 ttcatttgca tcgattgccg gaatttctgg tttgtctgtc aaaactgact aatttaaagg
    26461 ttaaaaaata aaaatctcac cattttgatg tacaagtatt cttgttttgt gcgttcaaca
    26521 aaatttacaa tattgaagca tttagaaaaa ttgactcaaa aaatggactg caaagatacg
    26581 actaaatcaa ttaaaatgaa ggaaatagct ttaaaatatg ttcatttccc tataaattaa
    26641 tgcgatttta acaagaatag tcgtttttta acaggtgata tttacttttt gcaattacaa
    26701 ctattattga atcaatcacc tattgagcta aaatatgtcg gtaaaagaca ttatttacat
    26761 ttaaaacctt aacaaatcat ttactattaa taacagcgat attcacgaat aagcacgtat
    26821 ttttgcgcca aatttccgtc agtttatgaa aaaaataaaa agtgcattaa tctctgttta
    26881 ctacaaagat ggtttagatg ttttaataca tacacttgct aagcaaggtg ttgtgtttta
    26941 ctcgacaggc ggcacgcaag catttatcga aaatctagga tatgcagtaa ttccagtaga
    27001 agaactaacg tcttatccaa gtattttagg tggacgagta aaaacgctgc atccaaaagt
    27061 ttttggcgga attctagcac gcagagaaaa tgcagatgat atagcacaac taaaacaata
    27121 tgcaattcct gagctcgatt tagtagttgt ggatttgtat ccttttgaag aaactgtaaa
    27181 aaatcaaagt acactttttg caggtgagct aacgtccgaa attatagaaa aaatagatat
    27241 tggcggcatt tccttaatac gtgcagcagc caaaaacttc agcgatacgg taatcgtttc
    27301 tcagaaaaat caatatggca ttcttgctca atttttagaa gaaaacgacg gtaatacaac
    27361 actgagtttt cgtcaggaat ttgcaaagca agcatttgct gtaagctcac attacgacac
    27421 gctcattcat cgttattttt cattgcaaca agcgaatgaa acctttaaag aaagctactt
    27481 acaaggcgag acactgagat atggtgaaaa tccacatcaa gctggttttt attatggaaa
    27541 tatcaatgaa gtatttgata ttttaaatgg caagcaatta tcgtacaata acttagtgga
    27601 tattgatgct gcggtacatt tgataagtga atttacagaa acaacatttg ctgttttaaa
    27661 acacacgaat gcttgtggcg tagccagtaa agcaagcgtt ttagaagcat ggaaagcagc
    27721 gttcgcagct gacactatat cggcttttgg tggtgtttta attacaaatg ccagcatcga
    27781 catagcaacg gcagaggaga tagataaatt attttttgaa gtattgattg caccagattt
    27841 ttcagcagaa gccttgcaaa tattaatgaa aaagaaaaat agaatcttgc taaaacaaaa
    27901 actatttaat tttccaaata aacaattcaa atccttattg aatggtgtaa ttgcacaaga
    27961 ttttgataaa aaaattgaaa gtagcgatga gctaaaaacc gttactacta ttgcacctac
    28021 atctcaacaa gtggaggatt tattatttgc caataaaatt gcaaaacatc taaaatcgaa
    28081 taccatcgtt ttagcaaaag acaagcaatt aattgcaatg ggctgtggtc aaacttcacg
    28141 cgttgatgcc ttacaacaag ccatacacaa agcaaccact tttggcttta gtttacaagg
    28201 tgctgttatg gcaagtgatg ccttttttcc atttcctgat tgcgtggaaa tagctgataa
    28261 agctggtatt tctgcggtta ttcatccagg aggaagcatc aaagatcaag attcaattga
    28321 ctattgcaat gcacatcaaa tgacgatggt attgacaggt attagacatt ttaaacatta
//
LOCUS       DAOQQV010000023        28233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_498_length_28233_cov_8.354745, whole genome shotgun sequence.
ACCESSION   DAOQQV010000023 DAOQQV010000000
VERSION     DAOQQV010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 28233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28233
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_28233_cov_8.354745"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..2821)
                     /gene="carB"
                     /locus_tag="PLL59_04140"
     CDS             complement(5..2821)
                     /gene="carB"
                     /locus_tag="PLL59_04140"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HMY23028.1"
                     /translation="MPKDTSIKSVLIIGSGPIIIGQACEFDYSGSQASRSLREEGIEV
                     SLINSNPATIMTDEVTADHIYLKPLTIDSIREILEERQIDAVLPTMGGQTALNLCKEA
                     SEIGLWEKYNVRVIGVDLNVIETTENREAFRKYCIDLGLGVAPSFVANSMLEGKEAAQ
                     KIGFPLVIRPSYTLGGSGGGFCHRAEEFEHMLANGLKASPIHEVLVEKAVLGWKEFEL
                     ELLRDINDSVVIICTVENLDPMGVHTGDSITVAPAMTLPDPVFQEMRNQAQKMMRGLG
                     SFAGGCNVQFSVNPETEEIIVIEINPRVSRSSALASKATGYPIAKIAAKLAIGYNLDE
                     LNNQITKTTSACFEPALDYVIVKIPRWNFDKFYGCDSTLGLQMKSVGEVMGIGRNFAE
                     ALQKACQSLENNKIGLGADGKSLSKPEEILESLQHPSWDRIFKIKDAISLGVPLHSIQ
                     KLTGIDNWFLMQIQELVKLEKEIQKYNINNISKELLTSAKKKGYSDAQLAFLLGRISE
                     DEVYEKRKALGIKRVYKMVDTCAAEFEAKTPYYYSAFDEENESVRSEKKKIIVLGAGP
                     NRIGQGIEFDYCCVHGLLAIKEVGYEAIMINCNPETVSTDFDIADKLYFEPVFWEHLW
                     ELIEHEQPEGVIVQLGGQTALKLSKKLHERGIKIIGSSFDSMDLAEDRGRFSDMLKAL
                     NIPYPDYGTATDADEALAVANKVGYPVLVRPSYVLGGQRMRIVINDQECETSVINLLR
                     DIPDNVILIDHFLDRAKEAEIDAIYDGEQVQIMGVMEHIEPAGIHSGDSNSVLPPYSL
                     GPIIIETMKHYTEKLCKALEIRGLINIQFAIKGDKVYVIEANPRASRTTPFICKAYQI
                     PYLNVATKVMLGTHKLKDFSFTEKLSGYAIKEPVFSFDKFPNVSKELGPEMKSTGEAI
                     YFIKDLFDPYFRQLYKDKSMFLSR"
     gene            2957..6766
                     /locus_tag="PLL59_04145"
     CDS             2957..6766
                     /locus_tag="PLL59_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMY23029.1"
                     /translation="MKKYILLLIFSACLFLQKSMATHLVGGEMSYRCLGGNQYEITLT
                     IFRDDYYGQATLDPTARITIFRNGSNSVLRTEDINLLGGGGTILPLDANPCITNRPRD
                     VRIEKGIYKKVITLPANTLGYKIVYQRCCRNDAAANINNGLGSTYMVNIPNTNLCNNS
                     PTFDNQPPLFLCANSKLDFDMSATDADGDQLVYSLVAPLQGLDEFSPRICDAFDGCNL
                     QQSAPPPYDSIYYLDGYTSQEPLGPTSTIYINPTTGILTTIPKTVGLFVVGIQVQEIR
                     AGVVLSSNIRDFQFNVTNCSVNSSVPIVITDNSPNSAIQVNDTTFSNCQGNIVRFDNG
                     VNQRALSYFWDFGVPSLTNDTSNLKNPTYTYADTGTYYVTLIINKGNPCSDTSRIKVV
                     YYPGLNTNFGFTGACQNDLIQLSDSSSSPYNDVNHWQWILSAGDTSYVKNPTTQFNTS
                     GTFPITLISSTSRGCVGKTTKNINVNPKPKAAFAANFLCYRHNANFTDASTITSGTIT
                     RYHWNFGDGFSDTLKNASHTYNVFADSFAVRHIVTSSFGCKDTIIKKIKMDDTVKISY
                     TTSPASLCENIPITFTNTSSGGNPTAFQWIINNGAPINGNTATVTFPNSGTYLVKLIS
                     TNRCGNDTLTKTITINAGPSINLGADVVVCNKSTKTITALGAFDSVRWNTNATTSSII
                     LDGNKSPIAVSVYKNGCFNKDTVLVQKQIITPNFSNNFLCINKPIQFSNTTTINFGTI
                     QQYDWKFGDGQFANNIQHPAHAYATFGNYNVQLIATSTEGCKDTIVKSMLTDSLLLVD
                     FSTAETISCQRKAVNFINQTNGGVNNQYIWKIEHNQINTKNTTYTFLGTGLFPVKLIA
                     SNRCNVDSITKNIRILPRPNVSLGRDTILCKHQTTTFSVNAPSYDSIRWVNGSTSATQ
                     ITDGSINPFKVKVYVDGCVAEDTVQVVAPKFILQFSNDFICYQKPIILNNTSTISQGS
                     IANYHWNFGDGQTLNGIKNPTHTYTHFGPKTIELIATASLGACKDTIKKPLVMDDSIS
                     FSIRPTLQEICYGLSVQYTNQSSGGVNTNYTWTLNNSQAQTGNTASYTHVRPGANVIQ
                     LIASHRCGADSTKYSFSVLPLPRVNLGADSIIMCPGELKMIGINQASDSIFWSTGERN
                     VDSIAINGLQSPIRVQVYKNGCLATDSIMVSTNCDVFIPTAFSPNNDGYNDAFNMMNK
                     SVKEYTLKVFNRWGELVFETNDLNNSWNGMYKGAPCPVDSYTYIATGKRFNEEVFYLK
                     GTVSLLR"
     gene            complement(6767..8362)
                     /gene="nadB"
                     /locus_tag="PLL59_04150"
     CDS             complement(6767..8362)
                     /gene="nadB"
                     /locus_tag="PLL59_04150"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HMY23030.1"
                     /translation="MPDIVIIGSGIAGLSLAVKTANKFPDKKILVLTKAKEDDSNTQY
                     AQGGIAVVTDFHKDTFEKHIQDTLIAGDGLCNEDVVEIVIREGRQRVDELISWGTNFD
                     KSEAGEYKLGKEGGHSEFRVLHHKDITGWEIERALLDKVHKTPNIEISQHHFVIDLLT
                     QHHLGKIITRVTENIQCYGVYVLNLETNQIEKIESKIVVLATGGFGQIYRATTNPSIA
                     TGDGIAMLYRAKGRVGNLEFVQFHPTALYEPGVSPNFLITEAVRGDGAILRTKNGQDL
                     MATYDKRGSLAPRDIVARAIDNEMKKSGDDYMYLDCRHMDKDNFLHHFPNIYEKCKSI
                     GIDVFKDMIPVVPAAHYACGGINVDEYGRTSIKNLYACGECSNTGLHGANRLASNSLL
                     EAIVFSHRIFESICQALPTITFANEQFPEWNADGTSHPKEMVIITQSIKELKDIMSNY
                     VGIVRNNGRLKRAQERLYLLFKETEELYETTTISPQLLELRNMITIGYLITRSAMLRK
                     ESRGLHYTTDYPSHAKEAECTML"
     gene            8500..9168
                     /gene="deoC"
                     /locus_tag="PLL59_04155"
     CDS             8500..9168
                     /gene="deoC"
                     /locus_tag="PLL59_04155"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HMY23031.1"
                     /translation="MKLSKYIDHTLLKASAVEADIIQLCNEAIKYDFYAVCVNSCYVG
                     LCVEQLKNTAIKTAAVVGFPLGAMETHSKIFEAKNAINAGADEIDMVINIGFLKDKKY
                     SEVVAEMKAIKNVIGDKILKVIIETCYLSDEEKKIATQLVVDAGADYVKTSTGFGNGG
                     ANITDIQLMKAITQDKIKIKASGGIRDYETAIQYIENGVMRIGTSNGIQIVTNNSDSN
                     STTY"
     gene            9178..9876
                     /gene="deoD"
                     /locus_tag="PLL59_04160"
     CDS             9178..9876
                     /gene="deoD"
                     /locus_tag="PLL59_04160"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HMY23032.1"
                     /translation="MSIHIAAKKGEIAETILLPGDPLRAKHVADTFLDDVICYNEVRG
                     MLGYTGYYQGKRVSVQGTGMGIPSISIYVNELIREYDVKNLIRIGTAGAFQENLKVKD
                     IVLALAASTNSAVNWNKFQGSDFAPTANFDLLLKAYQTANSLQLNVKTGNILSSDEFY
                     DDNPDAFKKWAQYGVLCVEMETAALYTIAAKYRASALAILTISDSLITHESASAEERQ
                     SSFNEMVKIALGTL"
     gene            complement(9873..10457)
                     /locus_tag="PLL59_04165"
     CDS             complement(9873..10457)
                     /locus_tag="PLL59_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HMY23033.1"
                     /translation="MILIDDKLISDDVVEKQFVCNIKKCKGICCVEGDSGAPVEKSET
                     KILKKIYPKIKSYLLPEGIKAIEEQGTFVKEDDDEYTPYATPLIDGGACAYVNYEQDG
                     TITCAIEKAWKDGLVDFQKPISCHLYPIRLKPMDTVTAVNYDVWDICSDACKLGKNLK
                     VPVYVFLKDALIRKFGLEFYDTLDYAASQHKPTA"
     gene            complement(10480..11178)
                     /locus_tag="PLL59_04170"
     CDS             complement(10480..11178)
                     /locus_tag="PLL59_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMY23034.1"
                     /translation="MQGKLYLIPIILAENVIETIPAYIKDIIIATDIYIVENLKTARR
                     FIKSIYKEKNIDDCTFIELNKHDNYKFDDAILSEIFAGKNIGLMSEAGTPCIADPGNK
                     VVEIAHEMNIKVVPLVGPNSILMALMASGFNGQQFAFNGYLPIDANERKSKLQQMEMA
                     AKKGITQIFMDTPYRNQKLLSEMINTNKFDTMLCIACNITAKDEYIKTLPLADWKKHQ
                     PDINKKPCIFIFGK"
     gene            complement(11184..11363)
                     /locus_tag="PLL59_04175"
     CDS             complement(11184..11363)
                     /locus_tag="PLL59_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23035.1"
                     /translation="MQPKLGPKELTLLNRKIEYRYIVETDYKIIYSIDEATHLIKIAD
                     VFDTRQNPIKIEREK"
     gene            complement(11500..11733)
                     /locus_tag="PLL59_04180"
     CDS             complement(11500..11733)
                     /locus_tag="PLL59_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23036.1"
                     /translation="MDIQTRKIAFVQSFLQLQSEETIAQLEELLVKLKKREKERKLRL
                     LSIKELNDRIAQSEKDFKNNKFKPSAELLAKYQ"
     gene            12113..12697
                     /locus_tag="PLL59_04185"
     CDS             12113..12697
                     /locus_tag="PLL59_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HMY23037.1"
                     /translation="MKLPITYYQNEDVVFLAKDLLGKTLITNINGIKTGGIIGETEAY
                     SEIEKGCHAFNKRKTERTKIMFEAGGLSYVYFCYGMHHLFNIVTGKKDFAQAVLIRAI
                     QPTIGIDEILKRRNKIKIDKNLCNGPGKVCQALGISKAHYGLSLTGNDIYVQTSKLES
                     AELQIISTTRIGIDYAAEDRYLPWRFLLVNKKNS"
     gene            12742..14568
                     /locus_tag="PLL59_04190"
     CDS             12742..14568
                     /locus_tag="PLL59_04190"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HMY23038.1"
                     /translation="MKYNSKKTIALFLLFIVLSTSIFAAAGGGGGGSSSGGGGGDGGG
                     EAIFYILYLLFRLIMLLPFPINVIVLIIIVFVVYKLSKTYQAGSGLNSIPNYSPANNH
                     NTYAGTDKLPKEFLIKNPDFNKEQFKQKVRTAFTEIQQAWMQQDLSKVRKWISDGVYQ
                     RFNTQFIMMKQLEQVNKIDDIFIEQVFIDDVETDGIYDIMHVGIQYSMYDAFESKKYK
                     RLNDGGPLNAMDYWSFIKKSGVKEKDLYHTTNCPNCGGNLPDDGGETAKCPYCATITY
                     LGDYDWILAEITQPDDYYNANAKYEKQGKFSIRIREQIGEKQDFSLQLLEDKASNGYM
                     QMMTALVFKKPEIARRFVSDMLYDKLTNRMQAEPAFVFNRLYLNHVTSFDYFKLNNKD
                     NIVVALKRSSQKIKNIDSDSPQYENSIFSQNEVLIMSRDVDANQPQGSLYAHSCPNCG
                     APIKDTIEVNCTYCNAQLNSTKYEWIISDWMSPNEYDAFKQANAASFVVDKKIDDLDE
                     LFKVRDYALNNVLMMIAADGRITNQEMKFVETLAKKWNYDLSKIQGFLSMAQNGKLVV
                     RMPQEEKQKAKIIALMEKAAILDNEITTAEKELLEQVKNISA"
     gene            complement(14565..14915)
                     /locus_tag="PLL59_04195"
     CDS             complement(14565..14915)
                     /locus_tag="PLL59_04195"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMY23039.1"
                     /translation="MHRNTLIISSFFLLLIIGLASFTHKEEEPIEWKNVQVLPSTITR
                     AQMDEVMDAWSASLGVNCSHCHTRGNYAADDKDEKIIARKMLVMTNEINEKYFGKDSG
                     TIGCMTCHNGKLHP"
     gene            complement(14993..15676)
                     /locus_tag="PLL59_04200"
     CDS             complement(14993..15676)
                     /locus_tag="PLL59_04200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMY23040.1"
                     /translation="MNKQILLFFLACICLFQTSFAANNSNKTSNTKMDTSTYVILTTE
                     YGDIKIKLYDETPQHKANFIKLVNEGTLDSTLFHRVIPEFMIQGGDPNSKNAQAGQAL
                     GMGSLGYRVPAEFNKNLIHKRGALSAARDNNPAKASSSCQFYLVQGKKYTRPELTILA
                     QRTGNSWTEDQLKVYEEVGGTPFLDMNYTVFGEVISGLDIIDKIAAAPRDPNDRPFKD
                     IRMTLKIEK"
     gene            complement(15681..16058)
                     /locus_tag="PLL59_04205"
     CDS             complement(15681..16058)
                     /locus_tag="PLL59_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23041.1"
                     /translation="MAHSVYLRVENTNATLAYSESEWQNVLNFIEKRFGKKPDLQTII
                     FLVGHRELGKHQVKFSKEQKQDLIHVGVCALLSNANYYTFLDFDEDGWPHYEYNRAMP
                     KLEHQQQEVLLKKMIIEYFKQIN"
     gene            complement(16075..16848)
                     /locus_tag="PLL59_04210"
     CDS             complement(16075..16848)
                     /locus_tag="PLL59_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HMY23042.1"
                     /translation="MSEQQNEINIALVQTDLAWKDKVANLNRIRQLVMTIKEPIDMIV
                     LPELFNTAFCVDDSSLAENMHGESISFLKEISKQQNCAICGSLLIHESEKVYNRFVFV
                     VDGEILFTYDKHYLFSLVIENQFLQAGAQKVTHKYKGWKIQPFICYDLRFPAWCQNNE
                     LADIQIYVASWPTKRIHHWQTLLRARAIENQCYTVGVNRTGKDGYGNAHNGHSCVFDF
                     TGNTCCDMTEKNGIEIVKLSATALSEHKERYPFWKDRLK"
     gene            16945..18975
                     /locus_tag="PLL59_04215"
     CDS             16945..18975
                     /locus_tag="PLL59_04215"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HMY23043.1"
                     /translation="MSTNKYPILLSELDLGFTKLKNRVLMGSMHTNLEEENNGFQKLA
                     SFYAARAKGGAGIIVTGGFAPNWEGWLKPFAGRMTNQGHVKKHKIVTEAVHSEGGKIA
                     MQILHAGRYGYHPLNVAPSRIQSPITPFKPRALSTWGVRRTINDFADSAALARDAGYD
                     GVEIMGSEGYLINQFIVQHTNKRTDEYGGSYENRIKFPIEIVRKAREKVGKDFIIIYR
                     LSMLDLIEDGSNWEEVVQLAKEIEKAGATIINTGIGWHEARIPTIATSVPRAAFSWVT
                     QKLKSEISIPLCTSNRINMPETAEQVLADGCADMISMARPFLADENWVVKAQSNKADE
                     INTCIACNQACLDHTFSNKLSSCLVNPRACHETELTYTASINKKNIAVVGAGPAGLAF
                     ASIAAERGHSVTLFDADKEIGGQFNMAKKIPGKEEFYETLRYFRKQLALHNVHVKLNT
                     RVQADDVKDFDEIVVATGISPRHIAIEGSNHSKVVSYIDVLKNDVKVGKKVAVIGAGG
                     IGFDVSEFLTQAGESTTLNKEEWIKEWGIDRKNEVRGGVAGIQQHIEAPAREVTMFQR
                     TEGKVGAKLGKTTGWIHRTTLKMKKVKMIAGVEYTKIDDEGLHYIDKNKMPQVLVCDN
                     VVVCAGQLSQRELEQPLKDLGKTVHVIGGAYEAGELDAKRAINQAARLAAVV"
     gene            18999..19406
                     /locus_tag="PLL59_04220"
     CDS             18999..19406
                     /locus_tag="PLL59_04220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMY23044.1"
                     /translation="MDIEAFKQSFSKANFINELGCKLIDIGNGTCEIHLPIQEKLWQQ
                     NGFVHAGVLATLADHVAGCAAYTVAKANTKVLTSEFKINLMRPAIGQKLIAKGKVLKP
                     GKLISVCQSEVYASNDGIEKLVAIALVSITNVE"
     gene            complement(19546..20406)
                     /locus_tag="PLL59_04225"
     CDS             complement(19546..20406)
                     /locus_tag="PLL59_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23045.1"
                     /translation="MKTFLITSRLIAIFLVLTFGLTSCNKSDLTNDDETEASQGLNIA
                     EIEDDNTQLMADQAESSGSVSDLRLISPNDNSDADILGGCAVVTKDTVSSPKSITIDF
                     GDGCTGPGGKVRKGKIIITYTGAYRAPGTAIHVVSENYFVNDKKIDIDKTIKNLGPNE
                     QGNLVFDVQAERIVTFPNGSSNKSTVHKRREWLAGASTPLDFSDDIFRVIGEGTHISR
                     RGVTYQVHTLTALIRKVSCHEFVSGELKIVRQADNRRFAIINFGTGECDDEATVTLDN
                     GRSFTIDLRH"
     gene            20620..20844
                     /locus_tag="PLL59_04230"
     CDS             20620..20844
                     /locus_tag="PLL59_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23046.1"
                     /translation="MKTITVSVPDEKVASLEAFLYQSNIKIIKEKEDLLELRILEIEM
                     EAAEPETPINTAAIEQALDEEIWKLHRFGE"
     gene            20953..21171
                     /locus_tag="PLL59_04235"
     CDS             20953..21171
                     /locus_tag="PLL59_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23047.1"
                     /translation="MKKITITVPDDKAEEVEQFIKQFIQQLNISILKEDEKLKVEEWQ
                     RDTVVKDETSDAYISSDEIEKTSFSREK"
     gene            complement(21226..21648)
                     /gene="ybeY"
                     /locus_tag="PLL59_04240"
     CDS             complement(21226..21648)
                     /gene="ybeY"
                     /locus_tag="PLL59_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HMY23048.1"
                     /translation="MAISFLFNTTVKKPAFFKASVIKTWLKQIAKNEQFNIQDLNVVF
                     MSDEELLQMNIDYLNHNTYTDIITFDNAEIKGKIESDIFISLERVEANAKKFKVSFEN
                     ELHRVLAHGVLHLCGYKDKKKADIALMRKKEEESLAML"
     gene            complement(21687..22169)
                     /locus_tag="PLL59_04245"
     CDS             complement(21687..22169)
                     /locus_tag="PLL59_04245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMY23049.1"
                     /translation="MNSYFLTLGDINLPTIKTIHAKKTFLMTEVIHLKINSNLKNIAK
                     VESMIETLRVQYHINDQQYGNMLLASVEAVTNAIEHGNNLDEKKIVAIEAQKCESIFR
                     LSVRDQGNGFNPDRLPDPTTPEYLEQPDGRGVFLMRKLADEVLFFDEGRCVEMKFILS
                     "
     gene            complement(22206..25514)
                     /locus_tag="PLL59_04250"
     CDS             complement(22206..25514)
                     /locus_tag="PLL59_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23050.1"
                     /translation="MNNYELLVEKLDAFIRKFYLNKLLRGALLFAGILLGFYLFISLF
                     EYQLYLSSTIRKSLLFGFIGITIFAFGTLILKPLIHYLKLGQQISHEQAASIIGNHFA
                     DVKDKLLNILQLKHQSQNAYNKELIEASIQQKLETIKLVPFSNAVNLNENKKYLKYVV
                     PPLFILLFLLVAAPNILTESNTRLLNPNTRFAKKAPFEFVLQNKKMKMLQYDDIEIHV
                     QIKGKTVPNEVYINEDGKNYKMEKKAADLFSYRFTNLQKDMQFYFSAGEFNSDEHHIR
                     VLKKPVIANFTTQLHYPSYINKKDETIKNTGDLLVPVGSSVHWDFETSGTDNVKIAFD
                     GQLYHAVKNNNNQFTFSKKITRDTRYTVYVANHEIENGDSVSFTIAATPDNFPSISVE
                     KIQDSTQKDFAIFLGAVADDYGISRLEFHYTIKDENGKLIISKKQQLPLANKSISDFN
                     FQIDFAKYQLNNGEKMDYYFEVFDNDAVTGPKSTKSQLFVYDKPTINELEKQEYQNSE
                     DIKDNLSAATKSAAKLAAQIKEMKEKILSKKSLSWEDKKQLEEIQKKHQELTKELEAI
                     KDKYDENLKNQDEFKKPDEDILQKQEKLQEMLKDMLSDEMKDLMKQIEDILQKMEQKN
                     TFENLDKMELSNKDLKSELDKMEKLFKQLQLEQKAQETIDKLNALAKEQEQLAQQTKD
                     KKEAQSDLQKKQESLNKKMDNIKEDLKQLEQFNKENDNKLDTKDNEGDAEDIKENMEN
                     SSDELQQEQNDKAAKSQKSAAQKMQKMAQKMKSGLDKMQQDQAAEDIKMIRQLLENLV
                     KLSFEQEQLMNELKKTETESPKYTQIMQKQYDLRDDAKLIGDSLQALGKRQFQLQSFI
                     SDEMYKLNREIKKAIDNMEERNKIVASVAQQMAMTATNNLALMLSESLDNMQMQMNQS
                     KPGSGACKKPGGKGEKPSMSEMQKKLGEQLGKMQEGMKQGQDPKKMGKDFADAVQQQA
                     AIREALRQMKEQMSQNQKKGENGIGGVDEMIKKMDELERELATKKLSSESLKRHKEIE
                     TRLLELEKAQRNQEEDEKRQSKSAIEIPRKLPPNLEDYLQKRKSALELYQTVPPNLKP
                     FYKNLVEKYLRLVN"
     gene            complement(25587..27317)
                     /locus_tag="PLL59_04255"
     CDS             complement(25587..27317)
                     /locus_tag="PLL59_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMY23051.1"
                     /translation="MKHLLYAFICLFLFSCIGEQKREFKLRPAKGGRYYGGTFRTNEE
                     EYFKTLYPLNVTEVVAHRIGEQLFDGLVTFDDSTLAVIPQLAERWEIDETATKYTFYL
                     RKGVRFHDDKCFVDGKGREMKAQDVKYCFDRICFHNPADNQGFWIFKDVVKGANEYDS
                     LTAKKINPKEGVSGIKVIDDYTIQIELERPYAVFLARLALIFGKIYPHEAVEKYGNEI
                     RMHPVGTGPFYLKINKDNQVTFLARNPHYWKKDEFGNQLPYLDALRISYIKEKKNELM
                     EFSKGNSDLVYRFPLEMIDEIVDYKKNLKPAYKNFILQYKPQIALQYYGFQHQGKIFN
                     NINVRKAFCYAIDRQKVCDFTLKGTGFPAIYGTVPPGTGTFDSKLVRGYTYDAAKAQE
                     YMAKAGFPKGKGFPKVTLELNSGGSRNSQVAEALKKMLEETLNIEIDLLIVPWAQHTE
                     AVETAKIDFYRLGWVADYPDAESFLNLFHSKYVPDDINVKTYINSFRYKNKNFDKVFD
                     EALATTDEAKRNILYAKADQQVIDDAVVLPIYYDIDFRLLQANVRNCPQNASEQRDFR
                     EVYFVPMKGK"
     gene            27426..27767
                     /locus_tag="PLL59_04260"
     CDS             27426..27767
                     /locus_tag="PLL59_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23052.1"
                     /translation="MKNHVLLLMLLLATAILFATKPKTGVHSVSEFEKLYQSKEFPFN
                     KISDTTFQRLKSGIAFDSEGRFRGFLFIGGLRKELTYEEYIKFNELISGFQQDTSEED
                     KKEYERRRNLK"
     gene            complement(27975..>28233)
                     /locus_tag="PLL59_04265"
     CDS             complement(27975..>28233)
                     /locus_tag="PLL59_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23053.1"
                     /translation="IGAEITSSDPSTMNAVFELRGNNKITFTNNGGSSLTAKIVFDIL
                     NGDTNVSLAIGANQPGTMINIDNDAPIEIPVTPGGINLVTL"
BASE COUNT         8745 a         5047 c         5036 g         9405 t
ORIGIN      
        1 taatctacct gcttaagaac atgctcttat ccttatacaa ttgtctgaaa taaggatcaa
       61 ataaatcttt gataaagtaa atagcttcac cggtagattt catttcagga cctaattctt
      121 tgctaacatt tggaaattta tcgaaagaaa aaacaggctc ttttatagca tagccgctta
      181 gtttttctgt aaatgaaaaa tctttaagct tatgtgtgcc taacatgact ttagtagcca
      241 catttaaata tggaatttga taagccttac agataaatgg tgttgttctc gatgcgcgtg
      301 gattggcttc tatcacatac actttgtcac ctttgatggc aaattgaata ttaatcagcc
      361 ctcgaatttc taaagcctta cacaattttt cagtgtaatg tttcatggtt tcaataatga
      421 taggacctaa gctatatggt ggcaacacgc tgttactgtc gccactgtgt atgccagcag
      481 gctcaatgtg ctccattacg cccataattt gtacttgttc gccatcatag atggcatcaa
      541 tttctgcttc tttcgctctg tctagaaagt gatcaataag gatgacgttg tcaggaatat
      601 ctcttaataa attaatgaca gatgtttcgc attcttggtc gtttatcaca atgcgcatac
      661 gctgacctcc taatacataa cttggtcgta ctaatacagg atagccaact ttattggcaa
      721 cggctaatgc ttcatcagca tctgttgcgg tgccataatc aggatatgga atattcagcg
      781 ctttcagcat gtcagagaag cgacctctat cttctgccaa gtccatgcta tcaaatgaag
      841 agccaatgat ttttatgcct ctttcatgta attttttaga aagttttaat gcagtttgac
      901 cacctagctg cactatgacg ccttctggtt gttcgtgttc aatgagctcc cagagatgtt
      961 cccaaaatac aggctcgaag taaagcttgt ccgcaatatc aaaatcagta gaaactgttt
     1021 ctggattgca attaatcata atagcttcat agcctacttc tttaatagct agcaagccat
     1081 gtacacagca gtaatcaaac tctatacctt gtccaatgcg atttggacca gcacctaata
     1141 caataatttt tttcttttca gagcgaacac tttcattttc ttcgtcgaaa gcagaataat
     1201 agtaaggtgt ttttgcttca aattcggcag cacaagtgtc caccattttg taaacgcgtt
     1261 tgatgcctaa cgcttttcgt ttttcataca cctcatcttc cgaaattcta cctaacaaaa
     1321 aggcaagctg tgcatcagaa taaccttttt tcttggcact tgtcaataat tccttagaga
     1381 tattattaat gttgtatttc tgaatttctt tttccagttt taccagctct tgaatttgca
     1441 tcaaaaacca attgtctatg cctgttagtt tttgaataga gtgtaaagga acacctaaag
     1501 aaatcgcgtc ttttatttta aaaattctat cccaacttgg atgctgtaaa ctttctaaaa
     1561 tttcttctgg cttagacaga cttttaccat cagcacctaa accaatttta ttgttttcta
     1621 gcgattgaca tgctttttgc aatgcctctg cgaagtttct gccgatgccc attacttcac
     1681 ctactgactt catttgcaaa cctaaagtag aatcgcaacc atagaatttg tcaaaattcc
     1741 aacgtggaat tttgacgata acatagtcta aggcaggttc aaaacaagca gaggttgttt
     1801 tggtaatttg attatttagt tcatctaaat tatagcctat agctagcttt gcggcaattt
     1861 ttgcaatagg atagcctgtt gctttagaag ctaatgcaga agagcgagac acacgcggat
     1921 tgatttcaat aacgatgatt tcttctgttt ctggatttac tgaaaattgt acattacaac
     1981 caccagcaaa actacctaaa ccacgcatca tcttttgtgc ttgatttctc atttcttgaa
     2041 aaacaggatc tggtaaggtc attgctggtg caactgtgat agagtcacca gtatgaacgc
     2101 ccatcggatc gagattttca acggtacaaa taataacaac actatcgttt atatctctta
     2161 ataattctaa ttcaaattct ttccagccaa gcactgcttt ttccactaac acttcgtgta
     2221 ttggcgatgc ttttaaacca ttagccaaca tgtgttcaaa ttcttcggct ctgtggcaaa
     2281 aaccaccacc tgaaccacca agcgtataag aaggacggat aacaagcgga aagcctattt
     2341 tttgtgctgc ttcttttcct tccaacatag agttggcaac aaatgaagga gcaacgccta
     2401 agcctaaatc gatacagtat tttctaaagg cttcccgatt ttctgtcgtt tcaatgacat
     2461 ttaaatctac gccaatcaca cgcacattgt atttttccca aagtccaatt tctgaggctt
     2521 ccttacataa atttaaggca gtttgtccgc ccatagtagg caataccgca tcgatttgtc
     2581 tttcttctaa aatttcacga atagaatcaa tggttaatgg cttcaaatag atgtggtcag
     2641 cagtgacctc atcagtcata attgttgctg gattggaatt gataagcgac acttcaatac
     2701 cttcttcacg aagtgagcgt gatgcttgcg agccggaata gtcaaattcg caagcttgtc
     2761 caataataat aggtccgcta cctatgataa gtacggattt aatagatgta tcctttggca
     2821 ttttgggaaa aagtttgaca aaattacaaa aatcagctca aatttttaga ataaataatg
     2881 aggtcttgca ttaaataatt cagagtaaaa ggttaaatac ttcttgttag tgtgtcattt
     2941 tctctatttt tgcatgatga agaaatatat tcttttattg atttttagtg cttgtctgtt
     3001 tttacaaaaa agtatggcca cacatttagt aggtggcgag atgtcatatc gatgtcttgg
     3061 tggcaatcaa tatgaaatta ctttaacaat ttttagagac gattattatg gacaagcaac
     3121 gctagatcca acggctagaa ttacaatttt cagaaatgga agtaattcgg tattacgtac
     3181 agaagacatt aatctattag gtggaggcgg tacgattttg ccattggatg ccaatccttg
     3241 tattactaac agaccacgag atgtgcgaat agaaaaaggc atttataaaa aagtaattac
     3301 attacctgcc aatacgcttg gatataaaat tgtatatcag cgctgttgta gaaatgatgc
     3361 agcagcaaac attaacaatg gactaggaag cacgtatatg gtcaacatac cgaatacaaa
     3421 tctttgcaat aattcgccaa cttttgacaa tcaaccaccg ttatttttat gtgcaaattc
     3481 aaaattggat tttgatatgt cggccaccga tgctgatggc gaccaattgg tatatagctt
     3541 ggttgcgcca ctgcaaggat tagatgaatt tagtccacga atttgtgatg catttgatgg
     3601 ttgtaatttg cagcaatcag caccaccgcc atatgatagc atttactatt tagatggtta
     3661 tacttctcaa gagccattag gcccaacatc tacaatatat ataaatccga ctacaggcat
     3721 attgaccacc atacctaaaa cagtgggact atttgtcgtc ggcattcaag tacaagagat
     3781 tagagctggt gtagtgctga gttctaatat tcgagatttc caattcaatg ttactaattg
     3841 ttctgtgaat tcatcagtgc ctattgtaat tacagacaat tcgccaaatt cggcaattca
     3901 agtaaacgat actaccttta gtaattgcca gggaaatata gtgcgttttg ataatggcgt
     3961 aaaccagcga gcattatcct atttttggga tttcggcgtg ccatcactta caaatgatac
     4021 ttccaatcta aaaaatccga cttatactta tgcagataca ggcacatatt atgtgacttt
     4081 aataattaac aaaggaaatc cttgctctga taccagcagg attaaagtag tatattatcc
     4141 aggattaaac actaatttcg gatttactgg cgcttgccaa aatgatttaa ttcaactcag
     4201 tgactcttcg tcatctcctt ataatgatgt aaatcattgg caatggattt tgtctgctgg
     4261 cgatacttcg tatgtgaaaa atcctactac acagtttaat acaagtggca cttttcctat
     4321 tacgctcatc tcttccactt cgcgcggttg tgtaggaaag acaacaaaaa atattaatgt
     4381 aaacccaaag ccaaaagcag cttttgctgc caatttttta tgctatcggc acaatgccaa
     4441 tttcacagat gcttcgacaa taacgtcagg caccattaca cgctatcatt ggaattttgg
     4501 cgatggattt tcagacacat taaaaaatgc cagccatacc tataatgttt tcgcagactc
     4561 atttgctgtg cgacatattg tgacaagttc atttggttgt aaggatacca ttatcaaaaa
     4621 aattaaaatg gacgatactg tgaagatcag ctacacgact tcaccggcta gtctttgcga
     4681 aaatatacct ataacattta cgaatacatc aagcggtggc aatccgacag cattccaatg
     4741 gattattaat aatggagcgc cgataaatgg caatacagcc actgttactt ttccaaatag
     4801 tggcacatat ctagtaaaat tgatatctac aaatagatgc ggtaatgata cattgacgaa
     4861 gacaattacc ataaatgctg ggccttcgat aaacttaggt gctgatgttg ttgtgtgtaa
     4921 taaatcaacg aaaacgatta cagcacttgg cgcatttgac tctgtgcgct ggaacacgaa
     4981 tgcaacaact tcatcaatta tattagatgg taataagtcg ccaatagcag taagtgtgta
     5041 taaaaacgga tgctttaata aagatactgt attggtacaa aaacaaatca tcacgccaaa
     5101 cttctccaat aattttctat gtatcaacaa gccaattcaa tttagtaata ctactacaat
     5161 caactttggc actatacaac aatacgattg gaaatttggt gatggtcaat ttgccaataa
     5221 tattcaacat ccagctcatg cctatgcaac ttttggaaat tataatgtgc aattaattgc
     5281 gacttctaca gaaggctgta aggatactat tgtaaaatca atgcttacag actctttgtt
     5341 gctagtagat ttttctacag cagaaacaat ttcttgccaa cgcaaagcgg tcaactttat
     5401 caaccaaaca aatggcggcg tcaataatca atatatttgg aagattgagc ataatcaaat
     5461 caatacaaaa aatacaacat atacattttt aggaacagga ttgtttcctg tgaaattaat
     5521 cgcgtcaaat agatgcaacg tcgattctat cactaaaaat attcgcattt tgcctagacc
     5581 caatgtgtca ttaggaagag atactatttt gtgtaaacat caaacaacaa cattttcggt
     5641 gaatgcgcca tcttacgact ctatacgatg ggtaaatgga tcgacatcag ccacgcaaat
     5701 aacagatgga agtatcaatc catttaaagt aaaagtatat gttgatggct gtgtagcaga
     5761 agacaccgta caggtagttg caccaaaatt tatacttcaa ttttctaatg attttatatg
     5821 ttatcagaag ccaattattc ttaataatac atcaacgata tcacaaggaa gtattgccaa
     5881 ttatcattgg aattttggtg atggacaaac attaaatggc ataaaaaatc caacacatac
     5941 gtatacgcat tttggaccca aaacaattga attaattgct actgcaagct taggagcttg
     6001 taaggatacg ataaaaaaac cattggtaat ggacgacagt atttcatttt ccattcgccc
     6061 gacactgcaa gaaatttgtt atggattgtc tgtgcaatat actaatcagt cttctggtgg
     6121 tgtgaataca aattacactt ggactttaaa taattcacaa gcacagactg gaaatacagc
     6181 aagctacaca cacgtgcgtc caggtgcgaa tgtaatacag ttaattgcat cacatcgctg
     6241 tggtgctgac tccacaaaat attcattttc ggtgttgcca ttaccacgag taaatttagg
     6301 tgcagacagt ataattatgt gtccaggcga gctaaaaatg ataggtatta atcaggcttc
     6361 agattctatt ttttggagca ctggtgagcg taatgtcgat tctattgcca tcaatggttt
     6421 acaatcgcca atacgtgtgc aagtgtataa aaatggctgc ttggcaacag actcaataat
     6481 ggtgtcaacg aattgtgatg tatttattcc gacggcattt tctccaaata atgatggtta
     6541 taatgatgcg tttaatatga tgaataagag tgtgaaagaa tacaccttaa aagtgtttaa
     6601 tcgctggggc gaacttgtgt ttgaaaccaa tgatttaaat aatagttgga atggcatgta
     6661 caaaggtgca ccatgtccag ttgatagtta tacgtatatc gctactggaa aacgttttaa
     6721 tgaggaagta ttttatctga aaggaacagt atccttgttg cgataactac agcattgtac
     6781 attcagcttc tttggcatgg cttggatagt ctgtggtgta atgcaggcca cgactttctt
     6841 tgcgtagcat ggcactacgt gtaatcaaat atccgatggt aatcatattg cgcaattcta
     6901 ataattgcgg tgaaatggtg gttgtttcgt acagttcttc tgtttctttg aatagaagat
     6961 agagcctttc ttgtgcgcgt ttcaaacgac cattattgcg aacaatacca acataattac
     7021 tcataatatc tttcagctct tttatacttt gcgtaatgat gaccatttct tttggatgtg
     7081 atgtaccatc agcattccat tctggaaatt gctcgtttgc aaaagtaatt gttggtagtg
     7141 cttgacaaat agattcaaat attctgtgtg aaaacacaat ggcttccaac agtgaattgc
     7201 tggctaatct gttagcgcca tgcaagcctg tattgctgca ttcaccacaa gcatataaat
     7261 tttttataga cgttctgccg tattcatcta cattaatgcc accacaagca taatgtgccg
     7321 caggcactac tggaatcatg tctttaaata catcaatacc tatggatttg catttttcat
     7381 aaatatttgg aaaatgatgt aaaaaattat ctttatccat gtggcgacag tcaagataca
     7441 tgtaatcatc gccacttttt ttcatttcgt tgtcaatagc acgtgcaaca atatcacgtg
     7501 gtgctaatga gccacgcttg tcgtaggtag ccatcaaatc ttggccattt ttcgtgcgta
     7561 aaattgcacc atcaccacga acggcttctg taattaaaaa gtttggactc acacctggtt
     7621 catataatgc ggtaggatga aattgaacaa actctaaatt tcctactctt ccttttgcac
     7681 gatacagcat cgcaattccg tcgcctgttg caatgcttgg atttgtcgtt gctctgtaaa
     7741 tttgtccaaa accgccagta gccaatacca caattttact ttctatcttt tcaatttggt
     7801 tggtttctaa gtttaataca taaacaccat agcactgaat attttcagtt acacgggtaa
     7861 tgatttttcc gagatgatgt tgtgtcaata ggtcgatgac aaaatgatgt tgcgaaattt
     7921 caatgtttgg cgttttgtgt actttatcta acaaggcacg ctcgatttcc cagccagtaa
     7981 tgtccttgtg gtgcaatact ctaaattctg aatgaccacc ttcttttccc agcttatatt
     8041 ctcctgcttc gcttttatca aaattggtgc cccaactaat taattcgtcc acacgctgtc
     8101 tgccttcgcg aattactatt tccaccacat cttcattgca taatccgtcg ccagcaatca
     8161 gtgtatcttg tatgtgtttc tcaaaagtat ctttatgaaa atctgtaaca acagcaatgc
     8221 caccttgtgc gtattgtgta ttgctatcat cttcttttgc tttagtaagt actaaaattt
     8281 ttttatctgg aaatttattt gcagttttta ctgctaagct taatccggct atacctgagc
     8341 ctataattac aatatctggc atgctgtaaa attaatagtt acatttagat atatgactga
     8401 taatttgata tttttttcat gaaaaatatc aaattataaa gtttactttt gatatgctac
     8461 ttcattggca aacgaataac ctaaatagga acaattagta tgaaactttc aaaatatatc
     8521 gaccacacat tattaaaagc tagtgcagtt gaagcagaca ttattcaatt gtgtaacgag
     8581 gcgataaaat atgattttta tgcagtttgt gtaaatagtt gttatgttgg tttatgcgta
     8641 gaacagctaa agaatacggc tataaaaaca gcagcagtag tcggttttcc attaggtgca
     8701 atggaaactc attctaagat atttgaagct aaaaatgcca ttaatgctgg tgccgatgaa
     8761 attgatatgg tcatcaatat tggtttttta aaagataaaa aatacagtga agttgtagca
     8821 gagatgaaag cgattaaaaa tgttattggc gataaaatac taaaagtaat tatcgaaact
     8881 tgttatttga gcgatgaaga aaaaaagata gctacacagt tagttgttga cgcaggtgca
     8941 gattatgtga aaacctctac cggctttggc aatggtggtg caaatatcac agacatacaa
     9001 ttaatgaaag ctattacaca agacaagata aaaataaaag ccagtggagg catacgcgat
     9061 tatgaaacag cgattcaata tattgaaaac ggtgtaatgc gcattggcac aagcaatggc
     9121 attcagattg ttacaaacaa ctcggattca aattcaacaa cgtattaaac tacaataatg
     9181 agtattcata tagcagcaaa aaaaggagaa attgcagaaa caatactact gccaggcgac
     9241 ccactgagag cgaaacatgt cgcagataca tttttagacg atgttatatg ctacaatgaa
     9301 gtacgcggaa tgcttggtta tacaggttat tatcaaggga agcgtgtgtc tgtgcaaggc
     9361 acaggcatgg gcattccttc catttcaatt tatgtcaacg aattaatacg agaatatgat
     9421 gtcaaaaatc tgatacgtat aggaacggca ggcgcatttc aagaaaattt aaaagtaaaa
     9481 gatatcgtgc tggcattagc agccagtact aattcggctg tgaactggaa taaatttcaa
     9541 ggatctgatt ttgcaccaac tgctaatttt gacttattgc tgaaagcgta tcagacggcg
     9601 aattccttgc aattaaatgt gaaaacgggc aatatacttt cttctgatga attttatgac
     9661 gacaatcctg atgcattcaa aaaatgggcg caatatggcg tgttatgcgt agaaatggaa
     9721 acagcggcat tatacacaat agctgccaaa tatagagcga gtgcattggc gattttaacc
     9781 attagcgata gcttaattac gcatgaatct gcttcggcag aagagcgtca aagctcgttt
     9841 aatgagatgg taaaaatagc attaggcaca ctttaagcag taggtttgtg ctggcttgct
     9901 gcatagtcta atgtgtcata aaattctagg ccaaattttc gaataagtgc atcttttaag
     9961 aaaacataaa caggcacttt caagtttttt ccaagcttac atgcatcgct acaaatgtcc
    10021 caaacgtcat agtttacagc ggtgaccgtg tccatcggtt ttagtcgtat tggatacaaa
    10081 tgacaactaa tcggtttttg aaaatcaaca aggccatctt tccatgcttt ttcaatggca
    10141 caagtgatag tgccgtcttg ttcgtagttt acataagcac atgcgccacc atcaattaat
    10201 ggcgtggcat aaggtgtata ttcatcatca tcttctttaa cgaaagtgcc ttgttcttct
    10261 atggctttaa tgccttctgg caataaatac gattttattt ttggatagat tttctttaag
    10321 atttttgtct ccgatttttc aaccggcgcg ccgctatcgc cttccacaca gcagatacct
    10381 ttgcactttt ttatattgca gacgaattgt ttttctacca cgtcatcgct aattaatttg
    10441 tcgtctatga gtatcatctt tttgcaattg tatatgtatt tatttcccaa agataaaaat
    10501 acaaggtttt ttattgatat caggttgatg ttttttccaa tctgctaatg gtaaagtttt
    10561 gatgtattcg tcttttgcgg taatgttgca agcaatgcaa agcatcgtat caaatttatt
    10621 cgtgttaatc atttcactta ataatttttg attgcgatat ggcgtatcca taaatatttg
    10681 tgtgatgcct ttttttgctg ccatttccat ctgttgcagt ttgcttttgc gctcatttgc
    10741 atcgatagga agatagccgt taaaagcgaa ctgctgtccg ttaaatccac ttgccatcag
    10801 tgccattagg attgaatttg ggccaacgag tggcactact tttatattca tttcgtgcgc
    10861 aatttctact actttgtttc caggatctgc aatacatggt gtgccagctt cactcattaa
    10921 gccaatattt tttccagcaa atatttcact taaaatagca tcgtcaaatt tgtagttatc
    10981 atgtttattt aattctataa aagtgcaatc gtcaatgttt ttttctttat aaatagattt
    11041 aataaagcgt cgcgcagttt ttaaattttc tacgatataa atatctgtcg caatgataat
    11101 gtctttaatg tatgcaggaa tagtttctat cacattttcc gccaatatga ttggaataag
    11161 ataaagtttg ccttgcattt gcattatttt tccctttcta tttttattgg attttgccta
    11221 gtatcaaaca catctgcaat tttgataaga tgagttgctt catcaataga atagattatc
    11281 ttatagtctg tttctacaat gtatctgtat tcaatttttc tattaagcaa agtaagttct
    11341 tttggtccaa gtttgggttg catttttaaa ggttcagttg cgagatgatt tgtttaatta
    11401 tatttttagc aactttaata tttacactgt ttttgtaata ttcaaaaatg tcgtctaatt
    11461 gtttttctga aaaagcagac caaattattt tgtatctcat tactgatatt ttgctaagag
    11521 ttcagcagat ggtttgaatt tattgttctt gaaatctttt tcagattgcg caattctatc
    11581 atttagttct ttgatagaaa gaagtcttag cttgcgttct ttttctcttt tttttaattt
    11641 tactaatagc tcttctaatt gagctatagt ttcttcactt tgcaactgca aaaaggattg
    11701 gacaaatgct atttttcgag tttgaatatc cataatcaaa tttataaaat aaaaatcatt
    11761 tttgatgagt aattatttta tacaggtttc ttcatctgcg cctgcaaaga ttctatcagt
    11821 agtttttggt tgttgattaa atcatctttg gctttcagca aagcttccca atgttttcgt
    11881 tcttcatcat tgtataatta tgaataatat cttacttccc aacatagcct cctgtaatgg
    11941 cctctgcgta aataaaagca gtacgcaacc ttcaaatagc gtcactttgt gagaatctga
    12001 ttagataaga ctaacaaact gcaataaggc aacttttttc tactaaacta tataaatatc
    12061 gccacttttt tctgtttaaa catagcagta tcttcgtttc tttgtactac acatgaaact
    12121 gccaatcact tactaccaaa acgaagatgt tgtttttctt gcaaaagatt tattgggcaa
    12181 gactttaata acaaatatta atggaataaa aactggtggc atcattggcg aaacagaagc
    12241 ctatagcgaa atagagaaag gctgtcatgc tttcaacaaa cgcaaaacag aacgcaccaa
    12301 aatcatgttt gaagctggtg gtttgtctta tgtttatttc tgttacggaa tgcaccattt
    12361 gttcaatatt gttacaggca aaaaagattt cgcacaagcg gttttaatta gagccataca
    12421 accaacaatt ggcattgatg aaattttaaa aagaagaaat aagataaaaa tagataaaaa
    12481 tttgtgcaac ggtcctggaa aagtatgtca agcattagga attagcaagg ctcattatgg
    12541 attatcattg acaggaaatg atatttatgt tcaaacctca aaattagaat cagccgaact
    12601 tcaaatcata agtactactc gtattggcat tgattatgca gctgaagata gatatttacc
    12661 atggcgattt ctattagtaa ataaaaaaaa cagttaaagg aactattttt gattttttta
    12721 tatttacaca tctgaatcac tatgaaatac aatagcaaaa aaacgatagc actatttctg
    12781 cttttcattg tccttagcac atctattttt gcagcagcag gtggcggcgg tggcggcagt
    12841 tcaagtggcg gcggcggtgg cgatggcggt ggcgaagcca ttttctatat tttatattta
    12901 ttattccgat taataatgtt gttgccattt cctatcaatg ttattgtgtt aatcatcatc
    12961 gtgtttgtcg tttacaaact ttctaagacc tatcaagcgg gaagtggctt aaattcgatt
    13021 ccaaattatt cgcctgccaa taatcataac acatatgcag gaacagataa gttgcccaaa
    13081 gaatttttaa taaaaaatcc tgatttcaat aaagaacaat tcaagcagaa agtaagaaca
    13141 gcctttacgg agatacaaca ggcttggatg caacaagatt tatcgaaagt ccgtaaatgg
    13201 atttctgatg gcgtgtatca gcgttttaat acacagttca tcatgatgaa acaattagaa
    13261 caggtcaata aaatcgatga catttttata gaacaggttt ttattgacga tgtcgaaaca
    13321 gatggcatat acgacattat gcatgtcggt attcaatact cgatgtatga tgcttttgag
    13381 tccaaaaaat ataaacgact aaacgacggc ggacccttga atgccatgga ttattggtct
    13441 ttcatcaaaa aatctggcgt caaagagaaa gatttatatc acacgacgaa ttgcccaaac
    13501 tgtggaggca atctgcctga tgatggcggt gaaacggcaa aatgtcctta ttgtgctacg
    13561 attacttact taggcgatta cgattggatt ttagcagaaa ttactcagcc tgatgattat
    13621 tataatgcca atgcgaaata tgaaaaacaa ggaaaatttt ctataagaat acgagaacaa
    13681 atcggagaaa aacaagattt ttctttgcaa ttgctcgaag ataaagccag caatggctat
    13741 atgcaaatga tgacggcttt ggtatttaaa aaaccagaaa ttgcaagacg atttgtatca
    13801 gatatgttgt atgacaaact tacaaatcgc atgcaagcag agcctgcatt cgtttttaat
    13861 cgattgtatt tgaatcatgt aacgtctttc gattatttta aattaaacaa caaagacaat
    13921 attgtcgtcg cattaaaacg ttcttcccag aaaataaaaa atattgatag cgattcgcca
    13981 caatatgaaa acagtatttt ctcgcaaaat gaagtcttga ttatgagccg agatgtcgat
    14041 gccaatcaac cacaaggttc attatatgcg catagttgtc caaattgtgg cgcaccaata
    14101 aaagatacga tagaagtaaa ttgtacctat tgtaatgcac aattgaacag cacaaaatac
    14161 gaatggatta tcagcgattg gatgtcaccc aatgaatatg atgctttcaa acaagcaaat
    14221 gcagcctctt ttgtcgttga taaaaaaata gatgatttag acgaattatt taaagtaaga
    14281 gattatgcat taaataatgt attgatgatg attgctgctg atggaagaat taccaatcaa
    14341 gaaatgaaat ttgtagagac attagcaaaa aaatggaatt atgatttgtc gaaaattcaa
    14401 ggatttttga gcatggcaca aaatggaaaa ttggtcgtgc gcatgccaca agaggaaaaa
    14461 caaaaagcca aaataattgc gttaatggag aaagcagcca tcttggataa tgaaattaca
    14521 acagcagaaa aagagttgtt ggagcaagta aaaaacatat cagcttaagg atgcagcttg
    14581 ccgttatgac atgtcataca gccaatagta ccactgtctt tgccaaaata tttttcattg
    14641 atttcattcg tcatgaccaa catcttgcgc gcgataattt tttcgtcttt atcatcggct
    14701 gcgtaattgc ctcgcgtatg acaatggctg caattaactc ctaatgatgc cgaccatgca
    14761 tccatcactt cgtccatttg tgcacgtgta atcgtggaag gcaatacttg tacgtttttc
    14821 cactcaatag gttcttcctc tttatgagta aaagaagcta atccaataat tagtagtaaa
    14881 aaaaatgaag aaataatgag tgtgttgcga tgcatatttt ttatcaatca agaatcttac
    14941 caaaataagt acgtcaatct tatgacgact gacgtacttt tatagaagtt ttttattttt
    15001 caattttcaa tgtcatgcgt atatctttaa aaggacggtc attaggatcg cgtggtgctg
    15061 cagcaatttt atcgataata tctaagcctg agataacttc gccaaagact gtgtaattca
    15121 tatctaagaa cggcgttcca cccacttctt catacacttt caattgatct tctgtccagc
    15181 tatttccagt gcgctgtgcc aatatggtaa gctctggacg tgtgtatttt tttccttgca
    15241 ccaaataaaa ctgacaagaa gaacttgctt ttgcaggatt attatcgcgc gctgcagaca
    15301 aagcgccacg tttatgaatt aaatttttgt tgaattctgc tggcacacga tagcccaaac
    15361 tgcccattcc gagcgcttgt ccagcttgtg catttttaga atttgggtcg ccaccttgaa
    15421 tcataaactc aggaatcacg cgatgaaata aagtagaatc gagcgtgcct tcatttacca
    15481 atttgataaa atttgctttg tgttgtggcg tttcatcgta tagttttatt ttaatgtcgc
    15541 catactctgt tgtgagtatt acataagtag aagtgtccat ttttgtgttg cttgttttat
    15601 ttgaattatt ggcagcaaag ctagtttgaa aaaggcaaat gcaagccaaa aaaaagagta
    15661 aaatttgttt gttcatatta ttaatttatt tgcttaaaat attcaataat cattttcttt
    15721 aacaggacct cttgttgctg atgctcaagt tttggcattg ctctattgta ttcataatgt
    15781 ggccagccat cttcatcaaa gtctaaaaat gtataataat tggcattact caataaagca
    15841 caaacgccta catgtatcaa atcttgcttt tgttctttgc tgaattttac ttgatgttta
    15901 cctagttcac gatgtcctac taaaaagata atggtttgta aatcaggctt tttgccgaag
    15961 cgtttttcaa taaaatttaa aacgttttgc cattcggact cactatatgc taaagttgcg
    16021 tttgtatttt ccacgcgcaa atatacagaa tgcgccatac ttaatcgatt gtttttattt
    16081 taatctgtct ttccaaaatg gatatctttc cttgtgttct gataaagctg tcgccgataa
    16141 ttttacaatt tctatgccat ttttttcagt catatcgcaa catgtattac ctgtaaaatc
    16201 aaaaacgcaa ctatgtccgt tgtgtgcatt tccataccca tcttttccgg ttctattaac
    16261 acctacagta taacattggt tttcaatggc acgtgcccgt aataatgttt gccaatgatg
    16321 aatgcgcttt gttggccagc tggccacata tatttgaata tctgccagtt cattgttttg
    16381 acaccaagcc ggaaaccgta aatcgtaaca aataaaaggc tgaattttcc accctttgta
    16441 tttgtgcgta accttttgag caccagcctg taaaaattga ttctctatta ccaatgaaaa
    16501 aagataatgc ttgtcatacg taaataatat ttcaccatca actacaaaaa caaatctatt
    16561 atacactttc tcagattcat gaattaacaa actgccacaa atagcacaat tttgctgctt
    16621 agatatttct ttcaaaaagg aaatgctttc gccatgcata ttttcggcta agctgctatc
    16681 atcaacacaa aatgcagtat taaataattc tggaagtaca atcatgtcaa taggttcttt
    16741 aatcgtcata actaattgac gtatcctatt taagttggcg actttatcct tccaagctaa
    16801 atctgtttgc actaatgcaa tgtttatttc attttgttgt tcgctcattt gtcatataaa
    16861 ttaccttttg ctaagtgctt ttttaataaa aaccaatgca agcattgaaa tattaattgt
    16921 atctttcaac aaacaaaaat acaaatgtca acaaacaaat atccgatttt attatcagaa
    16981 ctagatttgg gatttaccaa attgaaaaat agagtgttga tgggttcgat gcatacgaac
    17041 ttagaagaag aaaataatgg ctttcaaaaa ttagcatcat tttatgctgc acgtgctaaa
    17101 ggtggcgcag gtattattgt aacaggcggt tttgcaccta attgggaagg ctggttaaag
    17161 ccatttgctg gtagaatgac caatcaaggg catgtaaaaa aacataaaat tgtaacagaa
    17221 gccgtacata gtgaaggtgg gaaaattgct atgcaaatat tacatgctgg tcgttatgga
    17281 tatcatccat taaatgtagc gccatctcgc atacaatcgc caattacacc atttaagcca
    17341 agggcattgt ccacttgggg cgttcgcaga acgataaatg attttgcaga cagtgctgca
    17401 cttgctcgtg atgcaggtta cgatggtgta gaaattatgg gctcagaagg ctatttgatt
    17461 aatcaattta ttgtacagca taccaataaa cgaacagatg aatacggtgg tagctatgaa
    17521 aatagaatta aatttccgat agagatagtg cgcaaggcac gtgaaaaagt gggcaaagat
    17581 tttatcatta tttaccgttt gtcaatgctg gatttgattg aagatggtag caattgggaa
    17641 gaagtagtgc aattggcaaa agaaatagaa aaagctggcg caaccataat caatacaggt
    17701 attggctggc atgaagcgcg catacctacc atcgccacat cagtgccacg agctgcattt
    17761 tcttgggtaa cacagaagtt gaaatcagaa atatctattc cattgtgtac atcgaatcgt
    17821 atcaatatgc cagaaacggc agagcaggta cttgccgatg gttgcgcaga tatgataagc
    17881 atggcgcgtc catttttagc tgatgaaaat tgggtcgtga aagcacaaag caataaagct
    17941 gatgaaatca atacttgtat tgcttgcaat caagcgtgtt tggatcatac tttttccaat
    18001 aaattatcga gctgcttggt gaatccacgt gcttgccacg aaacagaatt gacttacaca
    18061 gcaagcataa acaagaaaaa tatagccgta gttggtgctg gtccggctgg tttagcattt
    18121 gcaagtattg ccgcagaacg cggacatagc gttactttat ttgatgcaga taaagaaata
    18181 ggcggacagt ttaatatggc gaagaaaatt cctggaaaag aggaatttta cgaaacattg
    18241 cgttatttcc gcaaacagtt agcattgcat aatgtacatg taaaactcaa tacaagagtg
    18301 caagctgatg atgtaaaaga ctttgatgaa atagtagtgg caacaggcat ttcgccacgc
    18361 catattgcca tcgaaggcag caatcatagc aaagtagtga gctatataga tgtgttaaaa
    18421 aatgatgtaa aagtgggtaa aaaagttgct gttattggtg ctggcggaat tggctttgac
    18481 gtaagcgaat ttttgacaca agcaggcgaa agcaccacat taaacaaaga agaatggatt
    18541 aaagaatggg gaatcgaccg aaaaaatgaa gtgcgtggcg gcgtggctgg catccaacaa
    18601 catattgaag cgcctgcacg tgaagtaacg atgttccagc gcacagaagg taaagtaggt
    18661 gcaaagctcg gcaagacaac aggctggatt catcgcacta ctttaaaaat gaaaaaagta
    18721 aaaatgatag ctggtgttga atacacaaaa attgacgacg aaggcttgca ttatattgat
    18781 aaaaataaaa tgccacaagt attagtatgc gacaatgtgg tggtgtgtgc tggacaatta
    18841 tcacaacgtg aactggaaca accattgaaa gacttaggta aaacggtgca tgtaataggt
    18901 ggcgcttacg aagcaggtga gcttgatgcg aaacgcgcaa tcaaccaagc agcgaggctg
    18961 gcagctgtgg tttaatttgt taatacataa taattgatat ggatatcgaa gcgtttaagc
    19021 aatcattttc aaaagcaaat tttatcaatg aattaggttg taaattaata gatatcggaa
    19081 atggtacttg cgaaattcat ttgccaatac aagaaaagtt atggcaacaa aatggctttg
    19141 tacacgctgg cgtattggct acactggccg accatgttgc aggttgcgct gcctacacag
    19201 tagcaaaagc caatacaaaa gtattgacat cagaatttaa aatcaatttg atgcgaccag
    19261 ccatagggca aaagctaata gcaaaaggca aagtgcttaa gcctggaaaa ttaatcagcg
    19321 tatgccagtc tgaagtatat gcaagcaatg atggaataga aaaattggta gctattgctt
    19381 tagtaagtat tacgaatgta gaataatatg ataacgctat tgaagaaaaa cagaatctaa
    19441 aaagaataca aattaaaaac aatcacacat caagttattc tcttcataaa aatcaaaatg
    19501 gctgtccttt tgagacagcc atttttacat aagtcaccca gtattttaat gtcttaaatc
    19561 tatcgtaaat gaacgaccat tatctaatgt aacggtagcc tcatcatcgc actcgccagt
    19621 gccaaagttg atgatggcaa aacgtctatt atccgcttgt cttactattt ttaattcgcc
    19681 acttacaaat tcatggcaac ttactttacg tatcaaagcc gtcaaggtat gtacttgata
    19741 ggtaacgcct cttcttgaaa tatgcgtgcc ttcaccaatc actctaaaga tatcatcaga
    19801 aaaatctaat ggtgtggaag cacctgctaa ccattcgcgt cttttatgta ctgtgctttt
    19861 attgctgctg ccatttggaa aggtgacaat acgttcagct tgtacatcaa aaactagatt
    19921 gccttgctca ttgggtccta aatttttaat ggttttatcg atgtcaattt ttttgtcatt
    19981 gacaaagtaa ttttcagaaa ccacatgaat cgccgtgcca ggtgctctat atgcaccagt
    20041 atatgtgatg ataatttttc ctttacgcac ttttccgcca ggtcctgtgc atccatcacc
    20101 aaaatcgata gtgatagatt ttggagaaga aacagtgtct ttggtaacta cagcgcagcc
    20161 accaagaata tcggcgtcgc tattgtcatt aggcgagatg agtcgtaggt cggaaacaga
    20221 gccactgctt tctgcttggt cggccattaa ttgcgtgttg tcatcttcaa tttcagcaat
    20281 gtttaaacct tgtgatgctt ccgtttcatc atcgttggtc aaatcgcttt tgttacatga
    20341 cgtgagtccg aatgtcaaca ctaaaaagat tgctattagt cttgacgtaa ttaagaatgt
    20401 tttcatgatt attttgtttt ggttagtaaa tagacttact ctgttataat aggtttaatg
    20461 acgatataat ttttaacaaa aaaaatgttg cataatgaaa ggtgaatgcc tacaacttga
    20521 attcaccatt cttcagtaca tgttttttgt cagtcgaaat tccctttttt gatataaatt
    20581 taaattcgaa atttttctaa tcataaaatt aaaaccatta tgaaaacaat taccgtttct
    20641 gttcctgatg aaaaagtagc ttctttggaa gcatttttat atcagtcaaa tattaaaatt
    20701 atcaaagaaa aagaagactt gttggaattg cgtattcttg aaattgaaat ggaagcagcg
    20761 gagcctgaaa caccaataaa tacagctgct atcgaacaag ccttagatga agaaatttgg
    20821 aaattacatc gctttggtga ataatttttt agtatttcac acgaataaaa ttatgctttt
    20881 taatagtgca taaggcaaat tgtattagtt gtattggaac taaaatctaa gttttttgat
    20941 taattttatt ctatgaaaaa aatcacgata acagtgcctg atgataaagc agaagaagta
    21001 gagcaattta tcaagcaatt tatacaacaa ctcaatattt ctattttgaa agaagatgaa
    21061 aaattgaagg tagaagagtg gcaacgcgat actgttgtaa aagatgaaac gagtgatgct
    21121 tatatcagta gcgatgaaat agaaaaaaca tcatttagta gagagaaata aaaatctaaa
    21181 tttttggcaa tcaatttttc gcgaagcaat attataatag cgattttaca acattgccaa
    21241 actttcttct tctttcttac gcatcagtgc aatatcggct ttctttttat ctttatagcc
    21301 acacaaatgc aatacgccat gtgcaagcac acgatgtaat tcattttcaa aactcacctt
    21361 aaattttttt gcatttgctt ccacacgttc caaggaaatg aagatatcgc tttcaatttt
    21421 cccttttatt tccgcattgt caaacgtaat aatatctgta tatgtgttgt ggtttaagta
    21481 gtcaatattc atttgtaata attcctcatc actcataaac acgacattca aatcttgaat
    21541 gttaaattgt tcatttttgg caatttgttt gagccatgtt ttaataacac ttgctttaaa
    21601 aaatgctggt ttttttacgg tagtattaaa aaggaaagaa atcgccatta ttctactttg
    21661 ctaaatttta gggcaaagat aatgcattat gataaaataa atttcatttc aacacagcgt
    21721 ccttcgtcga agaacagtac ttcgtcggct aattttcgca ttaaaaatac gccacggcca
    21781 tcaggttgtt ctaagtattc aggtgtggtt gggtcgggca gtcggtctgg attaaatcca
    21841 ttgccttggt cgcgaacgga aagcctaaaa atagattcgc atttttgcgc ttcaatggca
    21901 acaatctttt tttcgtccaa attattgcca tgttcgatgg cattcgtgac cgcttcgaca
    21961 gatgccaata gcatattgcc atattgctgg tcattaatat gatactgcac tctcaatgtt
    22021 tcaatcattg attctacttt tgcaatattt tttaagtttg aattaatttt taaatgaatc
    22081 acctctgtca tcaaaaatgt tttttttgcg tgaatagttt ttatggtcgg taggtttatg
    22141 tcgccaagcg tcaggaaata gctgttcatg caaacagaca aataattata cgtaagttta
    22201 atcaattagt taacaagtcg caagtatttt tctactaaat ttttgtagaa aggctttaaa
    22261 ttcggcggaa cggtctgata caattcgagt gcagattttc gtttttgcaa ataatcttcc
    22321 aaattaggcg gtagttttct aggaatttca atcgcagatt ttgattgtct tttttcatct
    22381 tcttcttgat tgcgttgtgc tttctcaagc tctaacagtc tggtttctat ctctttatgt
    22441 ctttttaatg attctgacga aagctttttt gttgctaatt cgcgttcgag ttcatccatt
    22501 ttttttatca tttcatcaac gccaccaatg ccattttctc cttttttctg attttggctc
    22561 atttgttctt tcatttgtcg caaggcttcg cgaatggcgg cttgttgctg tacggcatcg
    22621 gcaaaatctt ttcccatttt tttaggatct tgcccttgtt tcatgccttc ttgcatttta
    22681 ccgagctgct cgccgagttt tttttgcatt tcgctcatcg atggtttttc gcctttgcca
    22741 ccaggttttt tgcaggcacc gctgccaggc ttcgattggt tcatttgcat ttgcatgttg
    22801 tcgagcgatt cgctgagcat taaggccaag ttattggtag cagtcatggc catttgttgt
    22861 gccacactcg ccactatttt attgcgctct tccatattgt caatggcttt tttgatttcg
    22921 cggttgagct tatacatttc atcggaaata aatgactgca actgaaactg acgtttgcct
    22981 aaagcttgca aactatcgcc gatgagcttg gcatcgtcgc gcaagtcata ttgcttttgc
    23041 ataatttgcg tgtactttgg actttccgtt tccgtttttt tcagctcgtt catgagttgc
    23101 tcctgctcga aggaaagttt gaccaaattt tccaatagct gacgtatcat cttaatatct
    23161 tctgcggctt gatcttgttg cattttatct aagccagatt tcattttctg cgccatcttt
    23221 tgcatttttt gtgccgctga tttttgcgat ttggcagctt tatcgttttg ctcttgttgc
    23281 aattcgtcac tgctattttc catattttct tttatgtctt cggcatcacc ttcgttgtct
    23341 ttggtgtcga gtttgttgtc gttttctttg ttaaattgtt cgagttgttt gaggtcttct
    23401 ttgatgttgt ccattttttt atttaacgat tcctgttttt tttgcaaatc gctttgtgcc
    23461 tcttttttgt cttttgtttg ttgtgctaat tgttcttgtt ctttcgctaa tgcgttgagt
    23521 ttgtcaatgg tttcttgtgc tttttgctct aattgtagct gcttaaaaag cttctccatt
    23581 ttatcgagct ctgattttaa atctttgttt gataattcca ttttatctaa attctcaaac
    23641 gtattttttt gctccatttt ttgcaaaatg tcttcgattt gtttcatcaa atctttcatt
    23701 tcatcgctga gcatatcttt tagcatttcc tgaagctttt cttgtttttg caagatgtct
    23761 tcatcaggct ttttgaactc gtcttggttt tttaaatttt cgtcgtattt atcttttatc
    23821 gcttccagtt cttttgttaa ttcttgatgt tttttttgaa tctcttctaa ctgttttttg
    23881 tcttcccatg aaagtgattt cttgctcaat attttctctt tcatttcttt tatttgcgct
    23941 gctaatttgg cagcactttt tgtggcagcg cttaagttgt cttttatatc ttcgctgttc
    24001 tgatattcct gcttttcgag ctcattaatt gtcggcttgt cgtacacaaa aagttgtgac
    24061 ttggttgact taggtcctgt gacagcatca ttgtcaaaaa cctcaaaata atagtccatt
    24121 ttttcgccgt tgtttaactg atattttgca aaatcaattt gaaaattgaa atcggaaatg
    24181 gatttattcg ctaatggaag ttgttgtttt ttggagatga ttaattttcc attttcatct
    24241 tttatagtat aatgaaattc caaacgagat ataccataat catcggcgac agcacctaag
    24301 aaaatggcga aatctttttg agtagaatcc tgtatttttt ctacgctgat gcttggaaaa
    24361 ttatcaggag tggcagcaat ggtgaaggaa accgaatctc cattttctat ttcatgatta
    24421 gccacataca ccgtataacg cgtatctctt gttatttttt tagaaaaagt gaattgatta
    24481 ttattgtttt ttactgcatg atacagttga ccatcaaaag cgatttttac attatctgtg
    24541 cctgatgttt caaaatccca atgcacagaa gagccaacag gtactagcaa atcgcctgtg
    24601 tttttaatgg tttcgtcctt tttattgatg tatgatggat aatgcagttg tgtagtaaaa
    24661 ttagcgataa caggtttttt tagtacgcga atgtgatgtt catcgctgtt aaattctcca
    24721 gcagaaaaat aaaactgcat atctttttgc aagtttgtaa agcgatagga aaatagatcg
    24781 gcagcttttt tttccatttt gtaattcttg ccatcttcat tgatgtacac ttcgttgggc
    24841 acggtttttc cttttatttg cacatgtatt tcgatgtcat catattgcaa cattttcatt
    24901 tttttatttt gcaacacaaa ttcaaaaggt gcttttttag caaatcttgt attcggattt
    24961 aatagacgcg tattgctttc tgttaatata tttggtgcgg caacgagcaa aaacaataga
    25021 ataaacaaag gtggtactac atattttaag tattttttat tttcatttaa attaacggca
    25081 tttgaaaaag gcaccagctt tatagtttcc aatttttgtt gaatactggc ttcaattaat
    25141 tctttattat aagcattttg tgattgatgc ttcagttgta gaatattcaa caatttatct
    25201 tttacatcag caaaatgatt tccgataatg cttgctgctt gctcatgtga aatctgctga
    25261 ccgagtttta agtaatgtat taatggtttt aatattaatg taccaaaagc aaatattgta
    25321 attccgataa atccaaaaag tagagatttt cttattgtag acgaaagata tagctggtac
    25381 tcaaacagtg aaataaataa ataaaaacca agcaatattc ctgcgaacaa aagtgcacca
    25441 cgaagcaatt tattcagata aaattttctg ataaatgcgt ctaatttttc gacgagtaat
    25501 tcgtagttgt tcatccttgt taaatataaa aaaatgttgt gcgcaatgca ctattacaat
    25561 tgttatttta ttctaatact gttttattat tttcctttca tcggcacaaa atagacttcg
    25621 cggaaatcgc gctgttcaga agcattctgc ggacagttgc gcacgtttgc ttgtaataat
    25681 cgaaaatcga tatcgtaata aataggcaac accacggcat catctattac ttgctggtcg
    25741 gctttcgcat ataatatatt tcgctttgcc tcgtcagtag tagccaatgc ttcatcgaac
    25801 accttgtcga aatttttatt tttataacgg aaagagttga tatatgtttt tacgttgatg
    25861 tcatctggca catatttact atgaaataaa ttcagaaaac tttccgcatc aggataatcg
    25921 gcgacccaac ctaaacgata aaaatctatt ttcgctgttt ctacggcttc cgtatgctgc
    25981 gcccaaggca caatcaataa atcgatttcg atattcaaag tttcttctaa catttttttc
    26041 aaagcctctg ctacttgtga gttgcgcgaa ccgccagaat ttaattctaa tgttaccttt
    26101 ggaaagcctt tgccttttgg aaatccagct tttgccatat attcttgtgc ttttgcagca
    26161 tcatacgtat aacctcttac taacttagaa tcgaaggtgc cagtacctgg cggcacagtg
    26221 ccgtaaatag caggaaatcc agtacctttt aatgtgaagt cgcacacttt ctgacggtcg
    26281 attgcatagc aaaatgcctt gcgcacattg atgttattaa atatttttcc ttgatgctga
    26341 aagccataat attgcaatgc aatttgtggt ttatattgta aaataaaatt tttataagct
    26401 ggttttaaat tctttttata atctactatt tcatcgatca tttccaatgg aaaacgatat
    26461 actaaatcgc tatttccttt ggaaaactcc atcaattcat ttttcttttc tttgatgtaa
    26521 gaaatgcgta atgcatctaa atagggcaat tgattgccga attcgtcttt tttccaataa
    26581 tgcggattgc gcgccaagaa agtaacctga ttgtctttat taatttttag ataaaatgga
    26641 cctgtgccaa caggatgcat gcgaatttca ttgccatatt tctctactgc ttcatgtgga
    26701 tatatttttc caaaaattaa agctaagcgt gccaagaaaa cagcatacgg acgttctaat
    26761 tctatttgaa tagtgtaatc atctattact tttatgccac ttacaccctc tttcggattg
    26821 attttttttg ctgttaaaga atcatattcg ttagcacctt ttactacatc tttaaaaatc
    26881 caaaaacctt ggttgtctgc cggattgtga aagcaaattc tgtcgaagca atattttaca
    26941 tcttgtgctt tcatttccct acctttccca tcaacaaagc acttatcatc gtggaaacgt
    27001 acgccttttc gcaaataaaa agtatatttt gtagctgttt catctatttc ccaacgctct
    27061 gctaattgag gtataacagc gagcgtgctg tcgtcaaaag taactaagcc atcaaaaagt
    27121 tgctcaccaa ttctatgtgc cactacttcg gtaacattta agggatacaa tgttttaaaa
    27181 tactcttctt cattggtgcg aaaagtgcca ccgtaatatc tgccaccttt tgcaggacgc
    27241 aatttaaatt cgcgtttttg ttctccaatg caggaaaaaa gaaataaaca gataaaagcg
    27301 taaagaaggt gcttcatgaa aataaattat ggctaaaaat acgaatattt aacggaagaa
    27361 attagttttc aaaacacaat aaaatcgtat aaatcgagtt atatttgaat aaataaatta
    27421 tagaaatgaa aaatcatgtt ctacttttaa tgctattatt agctacagca attctttttg
    27481 ctacaaaacc taaaacaggt gttcattcgg taagcgaatt tgaaaaacta tatcaatcga
    27541 aagaatttcc gtttaacaaa atttcagata ctacttttca gcgtttaaaa tcaggaattg
    27601 cctttgatag cgaaggccgg ttcagaggtt ttctgtttat tggcggattg cgtaaagagc
    27661 tcacatatga agaatatata aaatttaatg agttaattag cggttttcaa caagacactt
    27721 cagaagaaga taaaaaagaa tatgaaagac gcagaaattt aaagtaatta taaattattc
    27781 cttgatttgt tggttttttt gaccaacaaa ccatcaggat aatattcgtc tattgcgttt
    27841 ttattcacgc ttggcagttt gttggctgca agccaacaaa ggcagagagc atgcgtaaat
    27901 attctacttt caatgagtta aatttgttgg tacgccactt gctttactag gctgcatacc
    27961 aacaatggtg acaattacag agtaactaga tttattccac ctggtgttac aggaatttct
    28021 attggagcat cgttatcaat attaatcata gttccaggct gatttgcacc aatagcaagt
    28081 gatacatttg tatcaccatt caaaatatca aaaactatct ttgcagttaa acttgaacca
    28141 ccattatttg taaatgtaat tttattattt cctcttaatt caaataccgc attcattgtt
    28201 gatggatcag aagaagtgat ttctgctcca att
//
LOCUS       DAOQQV010000024        27967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_504_length_27967_cov_7.646604, whole genome shotgun sequence.
ACCESSION   DAOQQV010000024 DAOQQV010000000
VERSION     DAOQQV010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 27967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27967
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_504_length_27967_cov_7.646604"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /gene="tyrS"
                     /locus_tag="PLL59_04270"
     CDS             complement(<1..595)
                     /gene="tyrS"
                     /locus_tag="PLL59_04270"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMY23054.1"
                     /translation="MLNFVEELRWRGMLHDIMPETEELLNKGMVKGYIGFDPTADSLG
                     IGNMVQVMTLLHFQRCGHKPIALVGGATGMVGDPSGKSVERNMLSEEQLNYNLSCQKK
                     QLEKFLDFNCGENSAEIVNNYEWFKNFTFLDFIRETGKHITVNYMMSKDSVKNRLENG
                     MTFTEFCYQLIQGYDFYYLWKNKDIKLQLGGSDQWGNI"
     gene            858..2003
                     /locus_tag="PLL59_04275"
     CDS             858..2003
                     /locus_tag="PLL59_04275"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HMY23055.1"
                     /translation="MLVAEPPKYSNDFMYIGVGAHNFGKANAVVATTDDITAGYWNPA
                     GLVRIPTKMQFAFMHNEYFAGIAKYDYAGIAFPLDSNKHAFAVNFMRFGVDDIPNTLN
                     LFDADGSINYDNVRNFSVGNYGILLSYAYSILPNLSIGVTTKIVHNKAGGFAKSWGFG
                     ADIGALYTIKDWKIGLTIKDITTTFNAWSYSFTDKEKQVLAQTGNIIPKSSTEITLPR
                     IQLGVAYQKTFKDKIDLTAEIDWELTTDGQRNTLISTKKVSIDPRIGVELGLWKIFYL
                     RGGVNNFQKMYNTEGKKKFTAQPNIGAGFKYKNFVSVDYAYTDVGKSSESQFSHVVSL
                     VLGINKRKKKESTELINAPDATITTEPATSPTEQSTPSVTPIQNEIK"
     gene            2003..7027
                     /locus_tag="PLL59_04280"
     CDS             2003..7027
                     /locus_tag="PLL59_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HMY23056.1"
                     /translation="MKKFVHIILLIGLFTLLSKAQPYGNEWIDYSKTYYKFKIGKTSL
                     YRIPHSVLVSLGIPSNQLIGTSFKLIRNGKEVPLYVSNNNIFSTSDFIEFYGEKNDGK
                     PDSVLYKSPLDQPNPNQNIFADSATFFLTLDPFSINARIIQQANDLTALPTPEEYCYT
                     TTQHIANNPSQLYGIGRGFTVSPFFPQLYNSDYEVGEGAIADAFNNRIYFRFFNSTSG
                     GLTNLKQFVRSAEINAYFSARIATGSGNHIIKMTINSFDTTVNSFGPTTKIIQQTFPS
                     NLLNGNGLEIIGFDELATNSNFVGYAALQYPRLFNFENDTKALFAIKKNADVYLEVRN
                     LNAVSTTPVLYDIENKQRYVGITDTVLKFHLLETLKQREQVYLSAQDTISIPIIRKFS
                     ALTFTNFLSANQQGNFLIISNKKLKSSTNGVDNIEEYRRYRSSAAGGSYNAKTYFIDE
                     LDDQFAYGIPQHPLAIRNFVNFAIDKFSVTPQLAFIIGKGFEYPYNYNNQSIIDRSLV
                     QTYGEPASDNLLTARSAVLNFPQIGIGRLAVTNGNEIKIYLDKVKEYETKLNDTFNLS
                     QTPFNKMWMKNVLHLGGGNNNFEQQLFRSYLDNYKTIIESPRYGAIVSGLFKNSTDPI
                     QIAQNIFLDSLINTGVSLITFFGHSATTTTDFNLDPDHMDNAGKYHLMLSNGCFVGSI
                     FVDFKSLSERFVLTANKAAIGYIAPVTYAVADNLDAFSSDFYTRFSNSMYNDKIGSIL
                     KETSKAVILSGGLINPMIGEQMIFHGDPGLRVNTHYRPDYYIDQSSISFEPQNINATA
                     DSFLVKVVVKNLGAAVNDQYVVNIDRIFPNGQIQVTKVTVPSARNVDTVLLWIKSDRL
                     NGPGLNTFRIKVDYENTIAEYSELNNEVTVTKVILADDIIPIFPYEFCIVNDPNFELV
                     FSTANVFAGPNTYLFQIDTTEFFNSPLLTQTRVTQPGATIHWKPPVQLIQNKVYYWRG
                     TVDTTAGNPANWKNSSFLYNTSLSTGWNQSHYFQYLKNSFSTLNLKNDRIFRYPNTVR
                     TMQIRVGYQPPLDQLIEAYWDGYLIARNAWARKGFIFFVYDGNTGQNWQTYQIGNTCR
                     GPYSDITCNRYPTNVIEFVTNDLANGKTERQHVTDFLNSIPCNSYVLGYSFYNAGYSQ
                     WLADSTAPGDNNLYQAFENLGVSKIRQQSENNPFAFFLKTCDNSYTPIQIQRFAPNII
                     DTIFSFSGTWTKGSMESAIIGPAKDWKHFDMDWSSLESTFGDNAVVNLYGYDTIGTRT
                     LLRDDLVKGAVTPITIDARRYPFLQMQWLTKDDTTGTAPQMEYWRLYYDKAPEAVVNP
                     TIFFSKTRDTIASGENFNMTVAVENVTPIDMDSLLVRYIIKDATNNITILYKRFGPLP
                     GKQSRNISFDFNFPGFNFFGLNYVTVEVNPDDDQPEQFHFNNFAEFTVYVEKDKINPL
                     FDVTFDGRHIIDGEYVSANPEILFRLKDENKYLALNDTSGFLIYMYYPNNPTEPVLIA
                     KSQSDVTFIPATNLNDGRNNEARLIYKPMLTDGTYEIRAQGIDRSNNDAGKYDYRIKF
                     KVDSKPSITNVLNYPNPFSTSTQFVFTITGATVPEQLIIQIFSASGKVVKEITREELG
                     NLHVGTNVTDYKWDGTDNFGDRLANGVYFYRVMTRNIDGTTTEIKESSIDKYFKKGYG
                     KMYIIR"
     gene            complement(7097..8488)
                     /gene="lpdA"
                     /locus_tag="PLL59_04285"
     CDS             complement(7097..8488)
                     /gene="lpdA"
                     /locus_tag="PLL59_04285"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMY23057.1"
                     /translation="MNNFDLIVIGSGPGGYVAAIRAAQLGMNVAIVEKESLGGICLNW
                     GCIPTKALLKSAQVFEYLQHSKDYGIEVSDVKIKFDEVVQRSRNVAEGMSKGVQFLMK
                     KNNITIIYGYGKVLPGKRVEVTLNEGGIKIFKANNVLLATGGRAKQLLSLPIDGKFII
                     DYRKAMVLPKLPTNMVIVGAGAIGVEFAYFYQTLGVHVTLVEFYDSILPIEDKEIAKL
                     LEKNFSKKGMTILTSSRVLSSKIIDNKVQLLVNSKSGEQVIETEIVLSAAGVVSNIEN
                     IGLELCGITTENDKVKTNSFYQTNVEGVYAIGDIVKGQALAHVASAEGIIAVEHMAGH
                     RPQALDYMNIPGCTYCSPEVASVGITEQKARENGTEILVGKFPFSASGKAKAAGKSDG
                     FVKLIIDKKYGEILGAHFIGENVTEMIAEIIVARKLETTGMEILKSVHPHPTMSEAIM
                     EAAAAAYGEVIHL"
     gene            8569..9759
                     /locus_tag="PLL59_04290"
     CDS             8569..9759
                     /locus_tag="PLL59_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMY23058.1"
                     /translation="MDLKDKIKNLADAYYNELVAIRRHLHAHPELSFEEKETAAYISN
                     KLDELGIEHTTNIGGHGIVGIIKGKNPTIKTIALRADIDALPIEEKNNCDYASKNIGV
                     MHACGHDVHTTSLLGAAKILNELKEHFEGSIKLIFQPAEEKLPGGASIMIKEGVLKNP
                     AVEVIFGQHVLPQLETGKVAFKSGIAMASCDEIYLTVKGKGGHGAVPHFTIDPILIAS
                     HIVVALQSVVSRNANPIMPSVLSIGKFIANGATNVIPEEVKLEGTFRTFDEQWRKEAH
                     LKIKNICENITTSFGGICEVNIEKGYPFLKNDEQTTNASFALAQQYLGAENVEEMPAR
                     MTAEDFSYYSQEVPACFYRLGTGNIVKGITSSIHTPTFDVDENCLKIGSGLMAWIAIK
                     HLSQ"
     gene            complement(9804..10460)
                     /locus_tag="PLL59_04295"
     CDS             complement(9804..10460)
                     /locus_tag="PLL59_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMY23059.1"
                     /translation="MRNKLLMMLLATLFFVACNTTPKGDKATTTASQDVATASGVELS
                     TDSSSVIAFTGNGVGKNHPGKFKVVNGKITLTDGKLSAGTFTIDVKSLSLDEQGEMIQ
                     TKLKGHLLHSDFFDAEKFPTAKFDITNVEEYVATPTDKTVVDGANARISGNLTLKAVS
                     KNVTFPAKITIADNQLEALANFNIDRTQWNMNYGNDKSLKDKFISSEVNIKIAIKATK
                     "
     gene            complement(10672..11130)
                     /locus_tag="PLL59_04300"
     CDS             complement(10672..11130)
                     /locus_tag="PLL59_04300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY23060.1"
                     /translation="MTKIIKSTIADSDILTEITKKSKAYWGYSDVQMQNWSELLTITE
                     NYIETNPVYKLLHNDFTIGYYSYIYLNEKEIKLDNLFVLPSYIGKGFGKLLMNNFIAR
                     IKNTDINKITLESEPKAEKFYEYFGFHTIGHIETSIKDRYLPIMELNIQP"
     gene            complement(11185..11268)
                     /locus_tag="PLL59_04305"
     tRNA            complement(11185..11268)
                     /locus_tag="PLL59_04305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11232..11234),aa:Leu,seq:gag)
     gene            complement(11379..12629)
                     /locus_tag="PLL59_04310"
     CDS             complement(11379..12629)
                     /locus_tag="PLL59_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMY23061.1"
                     /translation="MRKLTFAFYVSFSVLTFPLFAQSDADRIKSLEEKVEMLTNQLGL
                     DKVDSTEEPSTPKLTLKEKYDQKLEKIKSKIPFEINGSVDVFVQTNFNGKQTNAVLNR
                     AFVNKANSFELGMINLKMSKKIKKVGFCADLGFGPRAETANNTLYSNTILAIRQLYVS
                     YAPTNWVEFTLGNFETFFGYEFIIPQANFNYSTSLAFQNGPFYHTGFKANFTVKKWNI
                     LAGFFNDTDTKSDNDRNKYVGAQVGYTADNGGVYLNFVGGNEPTTYMVNDSTEAPVYK
                     NYKNSYDLTGSIKLGKNKKGKLGVNVAYHQYRYKEEKSSKVTRNKYFTTYLYGQCDIN
                     EHASIGARAGYFYNPDNVALYVAGPAKHFFDFTLTSQINVIGGLKLIPEYRIDFGSDK
                     VFLDRKGNGSKIQNTLSLAAVYAF"
     gene            12965..17497
                     /gene="gltB"
                     /locus_tag="PLL59_04315"
     CDS             12965..17497
                     /gene="gltB"
                     /locus_tag="PLL59_04315"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HMY23062.1"
                     /translation="MSVTNKHKSLYLPEMEKDACGIGFVAHLRGEKSHRTVQDALTML
                     ENMEHRGACGCEENTGDGAGILLQIPHEYYAKETEKLGFTLPQAGMYGTGFIFFPQDE
                     TVRQQCKDFIAQTAAKLNFTILGYRKINTDNSPIGPTAQSAEPVVEQLFIKPEFNFKE
                     DIELERKLYVLRAHTVREINYILGGKDQFYYTNLSCHTVIYKGQLTTNQVRQYYPELN
                     DESVESALAIVHSRFSTNTFPRWRLAQPFRYIAHNGEINTLQGNLNWMRTREHLLECD
                     VFTKEEIDMFMPICYRDQSDSASLDNVIELLYLSGRSLPHAIMMLIPEAWQDNEQMED
                     WKKAFYEYHDCLMEPWDGPASVCFTDGKIIGATLDRNGLRPSRFVVYNDGRVVMSSEA
                     GALPTEEEKVVQKGRLQPGKMFIINMEEGRIISDDELKKNICTQHPYREWLNKHKITF
                     SDLPKSTIKEDKLDHRALQQQQLAFGYTSEDIKTIITPMAETAYEPIGSMGTDTPLAV
                     LSDQSQNLSNYFKQLFAQVTNPPIDPIRERMVMSTFASLGGASSILTTTENHCKQIAI
                     RQPILSNDDIAKLRYINHPDFKVITIQALFTASGKPGALERGIDRICKEAEEAVANGN
                     NIIIISDKGVNKDFAPIPTLLATGAIHHHLVRTKTRIDCSLVVESGDIREVHQMATHI
                     GYGATVINPYLAFQTIYNLNTDKLKHLGKSDEKLVENYTKASSNGLLKVFSKMGISTH
                     QSYHGSQIFEIVGISKQVVDKCFTGSISRLDGLGFDEIAKEVIKRHDLAFPEKESITQ
                     RLEVGGQYQWKRRGEVHMFNPDTIHLLQHASKKNDFGIYKNYAEKINNQAQKAITLRG
                     LLEFDMSKATAIDISEVEPAENILKRFATGAMSFGSISHEAHTTLAIAMNRIGGKSNC
                     GEGGEDEIRFEVKENGDSERSAIKQVASGRFGVTSYYLANADELQIKLAQGAKPGEGG
                     QLPGHKVDEWIGRVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANPNARINTKL
                     VAEAGVGTVAAGVAKAKSDVILISGYDGGTGASPISSIKHAGLPWELGLAEAHQTLVK
                     NNLRSRVVLQTDGMLRTGKDIAVATLLGAEEWGIATAALVVEGCIMMRKCHLNTCPVG
                     IATQDPILRARFTGDPQHVINFFTFIVQDLREIMAKLGFRTINEMVGQTQFLKRRDNI
                     THWKYKHLDLSPIIHKEEANESVGLFKQMRQAHPTDEAILDKKLVAAAQSSLDKKELS
                     TGQFTIINTDRTVGALLSYEISCKYKKEGLPEDTIQFKFNGSAGQSFGAFVTGGVKFE
                     LAGEANDYFGKGLSGGKLIVYPASIASFKPSENIIIGNVAFYGATSGEAYINGMAGER
                     FCVRNSGAKAVVEGVGDHGCEYMTGGLAIVLGKTGRNFAAGMSGGVAYVYDIDKDFPK
                     NVNKEMVELEGLDTEDIDTLKFYIERHLKYTNSEIAKNILDNWEITSKYFVKVMPTDY
                     KKVLLERKNKTNTVNV"
     gene            17576..19066
                     /locus_tag="PLL59_04320"
     CDS             17576..19066
                     /locus_tag="PLL59_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HMY23063.1"
                     /translation="MGKPTGFLEFTRELPKYKAPEVRIKDYNEIYLPFADEKTQKQAA
                     RCMDCGVPFCHNGCPLGNIIPEFNDATYNGDWKLAAEILLSTNNFPEFTGRICPAPCE
                     SACVLGINQPPVAIEHIEKSIIEKAFELNLIQPKLPSERTGKKVAVVGSGPAGMAAAA
                     QLNKAGHTVTVYERADKIGGLLRYGIPDFKLEKEIIDRRIEVMEKEGIQFVTNTNVGV
                     DITVEELNKNFDAVVLSGGSTVPRDLPIQGRAFKGVYPAMEFLSQQNKRVANITTTVD
                     HRGIAYENGDILATDKHVIVIGGGDTGSDCVGTSNRHGAKSVTQIELLAMPPQERHES
                     TPWPLWPMMLRTSSSHEEGCERQWSILTKEFIGNEAGELIGLKIVEIEWKTIDGRPQF
                     VEIEGTERVLPCDLALLAMGFVHPQHEGLLANLAVALDERGNINANNYKTNVDKIFTA
                     GDMRRGQSLVVWAISEGRECAREVDLFLMGESQLEAKDKGVLFVGE"
     gene            19358..19945
                     /locus_tag="PLL59_04325"
     CDS             19358..19945
                     /locus_tag="PLL59_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23064.1"
                     /translation="MKTLLNIAVLLLCISSNSFSQNDISISDFLQLRANNNYTVESRL
                     SALNIQPYDEEIIGSGRTQYTFQAENKSTPLQWVDFIYEQDAAWNNRISFQTQNLAQV
                     KKYLTEMKSLGFYFVAKKIVDRQAYEVYSDGSNTIELITSQSRKVYDNNLYVNFAFYS
                     AGEYEYAFASENEKYNVAQFNKEELFADLVGLPIK"
     gene            complement(20010..21053)
                     /locus_tag="PLL59_04330"
     CDS             complement(20010..21053)
                     /locus_tag="PLL59_04330"
                     /inference="COORDINATES: protein motif:HMM:NF007912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HMY23065.1"
                     /translation="MIYTTLPSTSIKVSKIALGTMTWGEQNTEAEAHEQLNYAVHDRG
                     INFIDTAEMYSVPSRAETQGSTERIIGTWLKYNNNRKDIILASKVTGPSAALKHIGTG
                     IKFDRKNITNSLHQSLQRLQTDYIDLYQLHWPERQTNYFGPLGYAHTSDDAVTNFAET
                     LSVLQDFIQQGKIRYIGISNETPWGMMQYIQCAEKYNLPKISTIQNPYNLVNRVYEIG
                     LAEMGIREHVGLLAYSPLGGGLLTEKYLHGNRPDGARFTRWANYFKRYAHPNTMKAVE
                     QYAALANEHHLSLAQMALAYINSKPFLTSNIIGATSMQQLKENIDAIHVQLSNDVIQA
                     IEEIHLAFPNPAP"
     gene            complement(21189..21953)
                     /locus_tag="PLL59_04335"
     CDS             complement(21189..21953)
                     /locus_tag="PLL59_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HMY23066.1"
                     /translation="MEHYNMNLTLKVWRQKNNKDKGGLVTYQVKNISSEMSFLEMFDV
                     LNEQLIEENQEPIAFDHDCREGICGMCSMYINGRPHGPWHGTTTCQLHMRAFKDGDTI
                     VVEPWRAQAFPVIKDLVVDRAAFDRIIQAGGYISVNTGVTVDANATPIPKTDADKAFA
                     AAACIGCGACVAACKNSSAMLFVSAKVSQLALLPQGQPERESRVLDMVAQMDKEGFGS
                     CTNTGACEAECPKEISIENIARLNREYVAASLKSEE"
     gene            complement(21960..23939)
                     /locus_tag="PLL59_04340"
     CDS             complement(21960..23939)
                     /locus_tag="PLL59_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HMY23067.1"
                     /translation="MALNSKIPAGPVEKKWEDYKGHVKLVNPANKRKIEIIVVGTGLA
                     GASAAATLGELGYKVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIK
                     GGDYRAREANVHRLAEVSVNIIDQCVAQGVPFAREYGGFLNNRSFGGTQVKRTFYAAG
                     QTGQQLLLGAYSALERQVALGNVKQYARHEMLDIVEIDGKARGIIARDLISGKLERHF
                     GHAVVLCTGGYGNVFYLSTNAMGSNVTASWKAHKKGAFFGNPCFTQIHPTCIPVSGDH
                     QSKLTLMSESLRNDGRIWVPKKKDDNRKANDIPEEERDYYLERRYPAFGNLVPRDVAS
                     RAAKERCDAGYGVGTTKQAVYLDFRFNLINKYGRAEASKLGIENPDEATLVRLGKEVV
                     KEEYGNLFDMYEKITGENPYEVPMRIYPAVHYTMGGLWVDYELMTSVQGLYALGECNF
                     SDHGANRLGASALMQGLADGYFVIPYTIGNYLADEIATKAISTDSPEFLAAEKNVADR
                     INALMNIKGTKSVESFHKRLGKIMWEKCGMARNEQGLTEAIAEIQALKKEFWSDVRIP
                     GSIEEFNTELDKAGRVADFIELGELMCRDALNRKESCGGHFREESQTEEGEAERDDVN
                     FKYVAAWEFVGESNWNLHKEELVYENIKIAARSYK"
     gene            complement(23970..24635)
                     /locus_tag="PLL59_04345"
     CDS             complement(23970..24635)
                     /locus_tag="PLL59_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HMY23068.1"
                     /translation="MAKEKFITSSIGKKIIVGLTGLFLISFLVVHVGINSTILINDNG
                     ETFNTAAHFMSHNWIIRVMEIGLLLGLLAHIGLTLRLHFENKAKRPVAYAVSAGNANS
                     KWYSRSMSVLGLLLLIFLVLHMGDFWYHTKVAIFTGAEENSFLEVKEALTKWYIVVPY
                     ILGFIALGYHLLHGFPSAFQTLGLNHKKYTPIIAALGTAFSIVVPGLFGAIALAIFFG
                     LVQ"
     gene            complement(24842..25105)
                     /locus_tag="PLL59_04350"
     CDS             complement(24842..25105)
                     /locus_tag="PLL59_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HMY23069.1"
                     /translation="MSIDNQFAQAQVDVKTLTKRPTDAQLLDLYALFKQATDGDNDTK
                     KPGMFDIKGQFKWNAWKDKAGMSKDAAKQKYIDLVSELLKAYA"
     gene            25200..26732
                     /locus_tag="PLL59_04355"
     CDS             25200..26732
                     /locus_tag="PLL59_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23070.1"
                     /translation="MKRLQFAILLSIPLCWFFFKLLQAYTHPSACSVPNFVYAENLEQ
                     LNRWCFDAYSKTRYDDSANFLYVFSLWLLIHFLKYTSIKAAFVVSTMSSLVSVYLIQR
                     IIDSRFFGVNLLLVGLFFMSTQIWAGVLGDEVLFQGMLLLLAVRSYWKQRYFWLMIWS
                     ALNIMGRPDTLFLVLPLIIASYTDIRELKERYVHKFIIARIRKTVLLLIVPVLLFFTY
                     RYLYFGKIMPYNWLHHSLETDKQFGIFNFEAFNYLKHYLRFYTLPLFIGVIFYFLKER
                     SDLALRYYALAIGLIVIPMIYVCTYSQDENLAYKNYYSIYLGLILLALLFIRDYRSIT
                     QGVATAIFVLFFGFKSSFEFLQKTLQSYNDNEFYIANDLSQINKGKVIVDYNTFISWL
                     TEWRTIYTNGRFTAGEKMLTKDEIYNAKADLIITENITDTAFYQKHYTLLRVPKNTRQ
                     FEQQRKPDNSLDQFFYKNSRKVQLPKNNNYHVLALKESKNYNEIIKILKSHGANTVQL
                     KK"
     gene            complement(26730..26801)
                     /locus_tag="PLL59_04360"
     tRNA            complement(26730..26801)
                     /locus_tag="PLL59_04360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26766..26768),aa:Arg,seq:cct)
     gene            complement(26870..>27967)
                     /gene="metG"
                     /locus_tag="PLL59_04365"
     CDS             complement(26870..>27967)
                     /gene="metG"
                     /locus_tag="PLL59_04365"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HMY23071.1"
                     /translation="PAILKAHENFILPDNVPANEFMNLEGDKISTSRNWAVWLHEYLQ
                     ELPGKEDELRYCLIANMPENKDAEFTWQDFQNRVNSELAANIGNFVNRVAVLMHKFYD
                     GDIPTTTSNEQEEVLNAITNLRDTIHVSISKFKFKDALSTVMSISSLGNKYLADNEPW
                     KLIKTDEQRTKIVMQNALFICKTLKEVLMPLLPNTAQKIENQFAGFNGKIKPAQILFE
                     KITDEQVALQIDKLNKSKIVQAPTNTDDSTGISPSFISIKSEITYDDFAKLDIRTGKI
                     LEAEKVPKADKLLKLLVDIGVEQRVIVSGIAAHFQADEIIGQEVSVLVNLAPRKLKGI
                     ESNGMILMAEDVNGKLYFVKADGVQAGNVIR"
BASE COUNT         9248 a         4881 c         4751 g         9087 t
ORIGIN      
        1 caatattccc ccattggtcg ctgccgccaa gttgtaattt tatatcctta tttttccata
       61 aataatagaa atcatatcct tgaatcagct ggtagcaaaa ctcggtaaat gtcattccat
      121 tttccaatct attcttgaca gaatctttgc tcatcatata atttacggtg atgtgtttac
      181 cagtttcacg aataaaatcc aagaatgtaa aattcttaaa ccattcgtaa ttattaacga
      241 tttcagctga attttcgccg caattaaaat ctaaaaactt ttctaattgt tttttctggc
      301 atgataaatt atagttcaat tgctcttcgg aaagcatgtt tcgttccaca cttttaccac
      361 ttggatcgcc taccattcct gtggctccac caactaaagc aataggtttg tggccacaac
      421 gttggaagtg taataaggtc atcacttgca ccatatttcc gatgcctaat gaatcagcgg
      481 ttggatcaaa tccgatataa cctttcacca tgcctttgtt taataactct tctgtttctg
      541 gcatgatgtc atgcagcata cctcgccaac gtaattcttc tacgaaattc aacataattc
      601 atttcaattt gtgcaaaaat acaacttgaa agatgtttaa tgtgaattat gaaagctaat
      661 ttttgatttt gttaataaaa gcatacaatt gcaactaaaa aggaagaaat attatctaaa
      721 tattaggata aattaggtaa atttaccttg ccttggtctg cttggtacaa ttccaataaa
      781 aatgaatcgt taaatacatg atgcttatta gaaataaact tttttacaca ctgtctatat
      841 ttatctgtct accactcatg cttgtagctg agcctccaaa atatagtaat gattttatgt
      901 atattggtgt aggtgcgcac aattttggga aggcaaatgc tgttgtggca actactgatg
      961 atattacggc aggctattgg aatccagctg gcttagtaag aattcctaca aaaatgcaat
     1021 ttgcttttat gcataatgaa tattttgctg gcattgcaaa gtatgattat gctggcattg
     1081 cttttccatt agatagtaat aagcatgcat ttgccgtcaa ttttatgcgt tttggtgtag
     1141 atgacattcc aaatacttta aatttattcg acgctgatgg tagcatcaat tatgataatg
     1201 tcagaaattt ttctgttggc aattatggta ttttattgtc gtacgcttat agcattttgc
     1261 ccaatctttc tattggtgta actacaaaaa tagtacataa taaagctgga ggatttgcca
     1321 aatcttgggg ctttggtgcc gatattggtg cactatacac gataaaagat tggaaaattg
     1381 gattaacgat aaaagatatt acaacaacat tcaatgcttg gtcatatagc tttacagata
     1441 aagaaaaaca agtattagca caaacaggaa acatcattcc aaaaagttcg acggaaatta
     1501 cgttgcctag aattcaacta ggtgttgcct atcaaaaaac attcaaagac aaaatagatt
     1561 tgacggcaga aatagattgg gaattgacaa ctgacggaca aagaaatacg cttatctcta
     1621 caaaaaaagt gagcatcgat ccgagaattg gcgtggagct cggattatgg aaaatattct
     1681 acctacgtgg tggtgtgaat aattttcaga aaatgtataa cacagaagga aaaaagaaat
     1741 ttactgcaca gccaaatatt ggcgcaggat ttaaatacaa gaattttgta tctgtagatt
     1801 atgcttatac tgatgttggc aaatcttcag agagtcaatt ttctcatgtc gtttcattag
     1861 tcttaggcat taataagcgc aagaaaaaag aaagcacaga actcattaac gcacctgatg
     1921 cgacaataac aactgaacca gcaacatcac caacagagca aagcacacca agtgtaactc
     1981 ctatccaaaa tgaaataaaa tgatgaaaaa atttgtacat attattttac taataggatt
     2041 gtttactttg ctaagtaaag cgcaacccta tggcaacgaa tggatagatt actcaaaaac
     2101 atattacaaa tttaaaatag gaaaaacgag tttatatcgc attccgcatt ctgttttggt
     2161 ttccttagga attccatcaa atcaattaat aggcacgagt tttaaattaa ttagaaatgg
     2221 aaaagaagtg ccactgtatg taagcaacaa taatattttt tctacaagtg attttataga
     2281 attttatggt gaaaaaaatg atggcaagcc tgattctgtt ttgtacaaat cacctttaga
     2341 ccaacccaat cctaatcaaa atatttttgc tgactctgca acattctttc ttactttaga
     2401 tccattttct attaatgcgc gaatcattca acaagctaat gacttgactg cattaccaac
     2461 gccagaagaa tattgttata ccacaacaca acatattgct aataatccat cacagttata
     2521 tggcattgga agaggtttta ctgtgtcacc attttttcct caattataca actctgatta
     2581 tgaagttggc gaaggtgcta tcgcagatgc atttaataac agaatttatt ttcggttttt
     2641 caacagcact tctggtggtc taacaaattt aaaacaattt gtacgctctg cagaaattaa
     2701 cgcttatttt agtgcaagaa tagctacagg aagtggtaat cacattataa aaatgaccat
     2761 caattcattt gacacaacag taaattcatt tggtcctaca acaaaaatta ttcagcaaac
     2821 atttccttct aatttactca atggcaatgg tctagaaata attggcttcg atgaattagc
     2881 tacgaactct aattttgtag gttatgctgc attacaatat ccaagattat tcaattttga
     2941 gaatgataca aaggcattat tcgccataaa gaaaaatgca gatgtatatc tcgaagtacg
     3001 aaatttgaat gctgtttcta ctacaccagt tttatacgac attgaaaata aacaacgcta
     3061 tgtaggtatt acggatacgg tattgaaatt tcatttactg gaaacactta aacaacgcga
     3121 acaagtatat ttgtcagcac aagacactat ttccattccc ataattagga aatttagtgc
     3181 gctaactttt acaaatttct taagtgcgaa tcagcaagga aattttttaa taatatctaa
     3241 taaaaaactc aagagcagta cgaatggtgt agataatatt gaagagtaca gaagatatcg
     3301 ctcaagtgct gctggtggtt cgtacaatgc aaaaacctat tttattgatg aattggatga
     3361 ccagtttgct tatggcattc ctcaacatcc attggcaatc cgaaattttg taaattttgc
     3421 gattgacaaa ttttcagtta caccacaatt agctttcatc atcggtaaag gatttgaata
     3481 tccttataat tacaacaatc aatctattat tgacagaagt ttagtgcaaa catatggcga
     3541 acctgcttct gataatttat tgactgcgcg ctctgcagta ctcaatttcc cgcaaattgg
     3601 cataggcagg ctggctgtaa ccaatgggaa tgaaataaaa atatatcttg acaaagtaaa
     3661 agaatacgaa acaaaattaa acgacacctt caacctttca caaactccat ttaataaaat
     3721 gtggatgaag aatgtattgc acttgggtgg tggtaataat aattttgaac aacaattatt
     3781 tagaagttat ttagataatt ataaaacaat aattgagtcg ccgagatatg gcgcaatcgt
     3841 gagtggttta tttaaaaaca gtactgatcc gattcagatt gcacaaaata ttttcttgga
     3901 ttctttaatt aatactggtg tttcattaat cacatttttc gggcactcag ccacaacaac
     3961 taccgatttt aatctcgacc ctgaccacat ggataatgct gggaaatatc atctaatgtt
     4021 gtctaatggc tgttttgttg gaagtatttt tgttgatttt aaatctttga gtgaacgttt
     4081 tgtgctaacg gctaacaaag ctgcaattgg ttatattgca cctgttacgt atgccgttgc
     4141 tgataattta gatgcttttt cgagtgattt ttatactcga ttttccaata gcatgtacaa
     4201 cgacaaaata ggttcaatac tgaaagaaac atcaaaagct gtaattctta gtggcggctt
     4261 aatcaaccca atgattggtg aacaaatgat tttccatggc gatccaggct tacgtgtaaa
     4321 tactcattat agaccagatt attacataga ccaaagtagc atttcatttg aaccacaaaa
     4381 catcaatgca acagctgact cttttttagt gaaagtggtg gtgaaaaatt taggagcagc
     4441 tgtcaatgac caatatgtgg taaatatcga tcgtattttt ccgaatggtc aaattcaagt
     4501 aacaaaagta actgtgccaa gcgcacgtaa tgtggacacg gttttacttt ggataaaatc
     4561 tgatagatta aacggacctg gtttaaatac gtttagaata aaagttgatt atgaaaatac
     4621 gattgcagaa tattcagaac taaacaatga agtcactgtt acaaaagtga ttttagctga
     4681 cgatatcatt cccatttttc catatgaatt ttgcattgtc aatgatccga attttgaatt
     4741 agttttttct acagctaatg tctttgctgg accaaacacc tatttattcc aaatagatac
     4801 gacagaattt tttaatagtc ctttgctaac acaaacgcgc gtcacacaac ctggtgctac
     4861 catacattgg aaaccgccag tgcagttaat tcaaaataaa gtgtattatt ggcgaggcac
     4921 agtagataca acggcaggca atccggcaaa ttggaaaaat agttcatttc tatacaacac
     4981 gtctttatct accggctgga atcagtctca ttatttccaa tatttaaaaa attcatttag
     5041 cacattaaat ttaaaaaatg acagaatttt ccgatatcca aatactgttc gcactatgca
     5101 aatcagagtt ggctatcaac caccattaga ccaattaatt gaagcatatt gggacggata
     5161 tctcattgca agaaatgctt gggcacggaa aggatttatc ttctttgtgt atgatggaaa
     5221 cactggacaa aattggcaaa cttatcaaat cggaaatact tgtcgtggcc catacagcga
     5281 tattacatgt aaccgatatc caacaaatgt gatagaattt gttaccaatg atttggcgaa
     5341 tggtaaaaca gaacgtcagc atgtaacaga tttcttaaac tccattccgt gtaattccta
     5401 tgtgttaggc tatagctttt acaatgctgg ttattcacaa tggctagcag acagtacagc
     5461 tcctggcgac aataatttat atcaagcgtt tgaaaattta ggtgtaagta aaatacgtca
     5521 acaatcggaa aataatccat tcgcattttt cttaaaaaca tgtgacaata gctatactcc
     5581 aatacaaata cagcgatttg cacccaacat aatagacact attttttcat ttagtggtac
     5641 gtggacaaaa ggcagtatgg agtctgcaat cattggacca gcaaaagact ggaaacattt
     5701 tgacatggat tggtcttcat tagaatctac ttttggcgat aatgcagttg taaatttata
     5761 tggttatgat acaattggta cgcgtacatt attgcgcgat gatttggtaa aaggtgcggt
     5821 aacaccaata actattgatg caagaagata tccatttttg caaatgcaat ggctgacaaa
     5881 agatgataca acaggcacag cacctcaaat ggaatactgg agattatatt atgacaaagc
     5941 acctgaggct gttgtcaatc caactatctt tttttcaaaa acacgcgata ctattgcttc
     6001 tggcgagaat ttcaatatga cagtagcagt tgaaaatgtt acacctattg atatggatag
     6061 tttgttggtg cgttatataa taaaagatgc aacaaataac attactattt tatataaaag
     6121 atttggacca ttgcctggaa aacaatctag aaatatcagt tttgatttca atttcccagg
     6181 atttaatttc tttggcttaa actatgttac cgtagaagtg aatcctgacg atgatcagcc
     6241 agaacaattt cactttaata actttgctga attcactgta tatgtagaaa aagacaaaat
     6301 caatccactc tttgatgtca catttgatgg cagacatatc atcgatggcg aatatgtatc
     6361 agcaaatcca gaaatattat ttaggttaaa agatgaaaat aaatatctgg cattaaatga
     6421 cacatctgga tttttaattt acatgtatta tccaaataat ccgacagaac ctgtattaat
     6481 tgctaaaagt caaagcgatg ttactttcat acctgcaaca aatctaaatg atggtagaaa
     6541 taatgaagca cgtttaattt ataaaccaat gctgactgat ggcacttacg aaatacgagc
     6601 gcaaggcatt gacagaagta ataacgatgc tggaaaatat gattatcgta tcaaatttaa
     6661 agtagattca aaaccttcta ttactaatgt tttgaactat ccgaatccat tttcgacttc
     6721 tacacaattt gtatttacaa ttacaggtgc tactgtacca gagcaattaa ttattcaaat
     6781 atttagtgcc agcgggaaag tagtaaaaga aattactcgt gaagaattag gcaatttaca
     6841 tgttggcacc aacgtaaccg attacaaatg ggacggcacg gacaactttg gcgaccgatt
     6901 agccaatggt gtttatttct atagagtaat gactcgaaat attgatggta caactaccga
     6961 aattaaagaa agttccattg ataaatactt taagaaaggc tatggaaaaa tgtatattat
     7021 cagataaata ataaaaaaca tgctaaaaaa aagcgaacaa ttttgttcgc ttttttttta
     7081 ttctataaaa gaaattttat aaatgaataa cttcgccgta cgcagcagca gcagcttcca
     7141 taatggcttc gctcatggtt ggatgtggat gaacggactt tagaatttcc attcctgttg
     7201 tttctaattt tctagcaaca ataatttcag caatcatttc tgttacattt tcgccgatga
     7261 agtgagcacc caatatttca ccatattttt tatcgataat taacttcaca aaaccatctg
     7321 attttcctgc tgcttttgct tttccacttg ctgaaaacgg aaattttccg accagaattt
     7381 ctgtaccatt ttctctggct ttttgttctg taatacctac gcttgctact tcaggcgagc
     7441 aataggtgca gcctggaata ttcatataat ctaatgcttg tggacgatgg ccagccatgt
     7501 gctcaacggc aataatgcct tctgcgctgg ccacatgtgc taatgcttga ccttttacaa
     7561 tatcaccaat agcataaacg ccttctacat tcgtttgata aaagctattc gttttgactt
     7621 tatcattttc agttgtaatt ccacatagtt ccaatcctat attttcaata ttagatacga
     7681 ctccagctgc acttaaaaca atctcagttt ctattacttg ctcaccactt tttgagttca
     7741 ctaatagttg tactttatta tcaataattt ttgaactcaa cacgcgtgaa gacgtcaaaa
     7801 tagtcattcc ttttttacta aaatttttct ccaataactt agcaatttct ttgtcttcaa
     7861 tgggcaatat tgaatcataa aattctacta aagtcacatg cacacccaat gtttgataaa
     7921 aataagcgaa ctccacacct atcgcgcctg cacctacaat aaccatattt gttggtagtt
     7981 tgggcaacac catggctttg cgataatcaa taataaattt tccatcgata ggtaatgaca
     8041 ataattgttt ggctctacca cctgtagcca acaacacatt atttgcttta aaaatcttta
     8101 ttccgccttc atttaatgtc acctcaacac gtttaccagg caacactttt ccataaccat
     8161 aaataatagt aatgttgttt tttttcatta aaaactgaac tcctttactc atgccttccg
     8221 ccacatttct actgcgttgt acgacttcat caaatttgat tttaacatca cttacttcga
     8281 ttccataatc tttcgaatgt tgtagatatt caaaaacttg cgcagatttt agcaaagcct
     8341 tagtaggaat acaaccccaa tttaaacaga taccacctag gctttctttt tcgactatgg
     8401 ctacattcat gcctaattgc gcagcgcgta ttgcggcaac atagccacca ggcccggagc
     8461 caattacgat taaatcaaaa ttattcatgc aataagttta ttattatttt aaatgaatag
     8521 tatgctaatg actatatttg caaataatca ttattcaaat ttagtattat ggatttgaaa
     8581 gataaaataa aaaacttagc agacgcatat tataatgagc ttgtagctat acgaaggcat
     8641 ctacatgcac atcctgaatt aagttttgag gaaaaagaaa cagcagccta tatttccaat
     8701 aagttagatg aattaggcat cgaacacact acaaacattg gtggtcatgg aatagttgga
     8761 attataaaag gaaaaaatcc aaccataaaa acaattgctt tacgtgctga cattgacgct
     8821 ttgccaatag aggaaaaaaa taattgcgat tatgcttcga aaaatattgg agtaatgcat
     8881 gcctgcggac atgatgtaca cacgacatcc ttattaggtg cagcaaaaat tttgaatgaa
     8941 ttaaaagaac attttgaagg tagcataaaa ttaatttttc aaccagcaga agaaaaatta
     9001 ccaggtggtg cttctatcat gataaaagaa ggtgtattga agaatcctgc tgttgaagta
     9061 atttttgggc agcatgtttt accacaatta gaaactggaa aagtagcttt taagtcaggt
     9121 attgcaatgg caagttgtga tgaaatatac cttactgtta aagggaaagg cggccatggt
     9181 gcagttcctc attttacaat agatcccata ttgattgctt cgcacatcgt cgtagccttg
     9241 caaagtgtgg tgagcagaaa tgctaatccg ataatgccaa gtgtgttgag tattggaaaa
     9301 tttattgcaa atggcgcaac caatgttatt ccggaagagg taaaattaga aggtactttt
     9361 agaacctttg acgaacaatg gcgcaaagag gcacacctaa aaattaaaaa tatttgcgaa
     9421 aatattacta caagttttgg tggaatttgc gaagttaata ttgaaaaagg atatcctttc
     9481 ttaaaaaatg atgaacagac aacaaacgct tcatttgcat tagcacaaca atatttaggc
     9541 gctgaaaatg tagaagaaat gccagcgcga atgacagctg aagattttag ttattattcg
     9601 caagaggtac ctgcttgttt ttatcgatta ggtactggaa atattgtaaa aggtattaca
     9661 tcatcaatac atacgccaac atttgatgta gatgagaatt gtttaaagat tggtagcggc
     9721 ttgatggcat ggatagccat taagcattta agtcaataaa aaaggcgctt ccaaagaagc
     9781 gccttaccac actatgaaaa aaactatttt gttgctttga tagctatttt aatatttact
     9841 tctgaagaaa taaatttatc ttttaatgat ttgtcattgc cgtagttcat attccactgt
     9901 gtgcggtcga tattgaaatt agcgagtgct tccaattgat tatcggcaat cgtaattttt
     9961 gctggaaacg ttacattttt acttactgct ttgagtgtaa gattgccgct aatacgtgca
    10021 tttgcgccat ctacaactgt tttatctgtt ggcgtcgcta catactcttc tacatttgta
    10081 atgtcaaatt ttgctgtcgg aaatttttca gcatcaaaaa aatctgaatg caaaaggtga
    10141 ccttttaatt tagtttgaat catttcgcct tgttcatcta agctcaatga tttgacgtca
    10201 atggtaaatg ttccagcact aagcttgccg tcggtgagtg taatttttcc atttactact
    10261 ttaaattttc ctggatgatt tttacctaca ccatttccag taaatgcaat tacagaagac
    10321 gaatccgtgc ttaattcaac accagacgct gttgctacat cttgcgatgc tgttgtcgta
    10381 gccttatctc cttttggagt cgtattacaa gcaacaaaaa acaaagttgc caataacatc
    10441 attaatagtt tatttctcat attcattcat ttaattagtt gcaaagataa ctattgtata
    10501 tacaataatc atgtaaaatt atgttaaaaa ttgtttttag tctttacctt acgaggtctc
    10561 taattttgta agtgagtact ttagattaaa ataaaatttc taatgaaaag agaatatgaa
    10621 tttccctcaa aattcaatac tgttgagtcg atgttcgtgt aagttgtttt attacggttg
    10681 aatatttaat tccattatag gtaaatacct gtctttaatt gatgtttcta tatgtccaat
    10741 tgtatgaaat ccaaaatatt cgtaaaattt ttctgctttt ggttcagatt ctaatgttat
    10801 tttatttatg tcggtattct taattctagc aataaaatta ttcataagaa gtttcccaaa
    10861 tccttttcca atatagctcg gtaaaacaaa tagattatcc agttttattt ctttttcatt
    10921 caaataaatg taagaatagt atccaatagt aaaatcatta tgtaataatt tgtaaacagg
    10981 attagtttca atgtagtttt ctgttattgt caataattca gaccaatttt gcatttgtac
    11041 atctgaatat ccccaataag ctttagactt tttagtaatt tcagtcagaa tgtcgctgtc
    11101 tgcaatagtt gattttatta ttttcgtcat attttattta caattagcga ctacataaaa
    11161 atttaacgaa aaatttggaa gatgtgccca agacaggact cgaacctgca caaccttacg
    11221 gtcactacca cctcaaagta gcttgtctac caatttcaac acttgggcaa taattgaagt
    11281 gtgcaaatat aaagattaat atgaaaaaaa gattgtgatt gttataaaca aaaaaacttg
    11341 gatgagacat ccaagttttt ttttgaatat tgattagatt agaaagcgta aactgccgct
    11401 aagcttaatg tattttgaat ttttgagcca ttaccttttc tatccaaaaa tactttatca
    11461 gaaccaaagt cgattcgata ttctggaatc aattttaaac caccaatcac attgatttga
    11521 gaagttaaag tgaaatcaaa gaaatgtttt gctggaccag caacatatag cgctacatta
    11581 tctggattat agaaatagcc agcacgtgca ccgatgcttg catgttcatt gatatcgcat
    11641 tgaccatata agtatgtcgt gaaatatttg ttgcgcgtaa cttttgaaga tttttcttct
    11701 ttgtatctat attgatgata tgctacattt acacctaatt ttcctttttt attttttcct
    11761 aatttaattg aacctgttag gtcataagag tttttatagt ttttataaac tggagcttcc
    11821 gttgaatcgt ttaccatata agttgttggt tcattaccgc caacaaaatt caaatacacg
    11881 ccaccattgt cagctgtata accaacttga gcacctacgt atttatttct gtcattatca
    11941 gattttgtat ctgtatcatt aaaaaatcca gctaaaatat tccatttttt tacagtaaaa
    12001 tttgctttaa aacctgtatg ataaaatgga ccattttgaa atgcaagaga tgtggaatag
    12061 ttaaaattag cttgtggaat gataaattcg taaccaaaaa atgtttcaaa atttcctaat
    12121 gtaaattcta cccaatttgt aggcgcatac gaaacatata attgtctgat tgctaaaatt
    12181 gtgttggaat ataaagtatt attagctgtc tcagcgcgag gaccaaaacc taaatcagca
    12241 caaaagccca cttttttaat tttcttagac atctttagat taatcatgcc caattcaaag
    12301 gagtttgctt tattgacaaa agcacgattt aaaacagcat ttgtttgttt accgttaaaa
    12361 tttgtttgca caaaaacatc aacagaacca ttaatttcaa aaggaatttt ggatttgatc
    12421 ttttccaatt tctgatcgta cttctctttc aaagtaagct ttggcgtgct cggttcttct
    12481 gttgaatcta ctttgtccaa tcctaattga ttagtcaaca tttcaacttt ttcttctaat
    12541 gacttaattc tatctgcatc tgattgtgcg aataaaggga aagtaagaac cgaaaaggaa
    12601 acataaaagg cgaatgtcaa ttttctcata gtgcaatttt taaatgttaa taaatattat
    12661 tttgcaagta taaaatacta ttttgatact ttttactaaa tatttttaga taattttaat
    12721 ttaattttag atttacaata ctattttcac aatattattg acatattttt tattataatg
    12781 tatggttaag ttagacaaac tcctttccct tttcgtaatt gttaagagat tttttaatgc
    12841 ttaagatttt cttaaacgct ataaaagaaa aagagaggaa gaatttatct aatattataa
    12901 actagtctga aaagctatct gaaatacaat atttctgtct ctaacaaaat tattatttaa
    12961 gaaaatgagt gttactaata aacataaaag tttatacctg cctgaaatgg aaaaagatgc
    13021 ttgcggcatc ggttttgttg cacatttaag aggtgaaaaa tcgcatcgta cagtacaaga
    13081 tgcgctaacc atgctagaaa acatggaaca tcgaggcgct tgcggttgcg aagaaaatac
    13141 aggcgatggc gctggtattt tattacaaat tcctcatgaa tactatgcaa aagaaacaga
    13201 aaaattaggt tttacgctac ctcaagctgg tatgtatggg actgggttta tcttctttcc
    13261 tcaagatgag acagtaaggc agcaatgcaa agattttatt gcacaaactg cagcaaaatt
    13321 gaatttcacc attttaggtt atcgtaaaat aaatactgac aattctccta tcggccctac
    13381 agctcaatca gcagagccag tggtagaaca attatttatc aaaccagaat ttaatttcaa
    13441 agaagatatc gaactcgaac gcaaactata cgtattgcgt gcacataccg ttcgtgaaat
    13501 taattatatt ttaggcggta aagatcaatt ctattatact aacttgtctt gtcatactgt
    13561 tatctataaa ggacagctga ccactaatca ggttcgtcaa tattatcctg aattaaatga
    13621 cgagagcgtt gagtcagcat tagcaattgt acactcacgt ttttctacca atacttttcc
    13681 gcgctggcga ttagcacaac cattccgtta tattgcacat aatggtgaaa taaacacatt
    13741 acaaggcaac ttaaactgga tgcgtacaag agagcatttg ttagaatgtg atgtattcac
    13801 caaagaagag atagacatgt ttatgcctat ttgctatcgc gaccaatctg actctgcatc
    13861 acttgataat gtaatcgaat tattatatct atcaggtcgt tctttgccac acgccattat
    13921 gatgttaatt cctgaagcgt ggcaagacaa tgagcaaatg gaagattgga aaaaagcatt
    13981 ttatgaatat cacgattgct taatggagcc ttgggacgga cctgcttctg tttgttttac
    14041 tgatggaaaa attattggag ccacattaga cagaaatgga ttgcgacctt cgcgctttgt
    14101 cgtatataac gatggcagag tcgttatgtc ttctgaagct ggcgcattac caacggaaga
    14161 agaaaaagta gtgcaaaaag gcagattaca gcctggcaaa atgtttatca tcaatatgga
    14221 agaaggaaga attatttctg atgatgaatt aaagaaaaat atttgcacgc aacatccata
    14281 tcgcgaatgg ttaaacaaac ataaaatcac attttctgat ttacctaaat ctacaattaa
    14341 agaagataaa ttagaccatc gtgcattgca acaacaacaa ttagcatttg gttatacttc
    14401 agaagatatt aaaacgatta tcacaccaat ggcggaaact gcttacgaac ctataggttc
    14461 aatgggcaca gacacacctt tagctgtact ttctgatcaa tcacaaaatc tatccaatta
    14521 ttttaagcaa ttatttgcac aagtaaccaa tccaccaatc gatcctattc gcgaacgcat
    14581 ggtgatgagt acttttgcat cactaggtgg cgcaagtagt atattgacaa caactgaaaa
    14641 tcactgtaag caaatcgcta ttcgtcaacc tattttgtct aatgacgata tcgcaaagtt
    14701 acgttatatc aatcatcctg attttaaagt aataactatt caagctttat ttactgcaag
    14761 tggtaagcca ggtgctttag agcgtggtat cgacaggatt tgtaaagagg cggaagaggc
    14821 tgttgctaat ggtaataaca tcatcatcat ttctgataaa ggagtgaaca aagattttgc
    14881 accaattcca acattactag cgacaggtgc aattcatcat catttagtac gcacaaaaac
    14941 acgtatcgat tgttctcttg ttgttgagag tggcgacatt cgcgaagtgc atcaaatggc
    15001 tacacatatc ggttatggtg caaccgtaat caatccttat ctagcattcc aaactatata
    15061 taatttaaat acagacaagc ttaaacatct cggcaaatca gatgaaaaat tagtagaaaa
    15121 ttatacaaaa gcatcaagca acggtctatt aaaagtattc tctaaaatgg gaatttcaac
    15181 gcatcaatct tatcatggtt cacaaatttt cgaaattgta ggtatcagca aacaagtggt
    15241 agacaaatgc tttactggtt caatctcaag attagatggt ttaggttttg atgaaatagc
    15301 aaaagaagta ataaaacgtc acgacttagc atttccagaa aaagaaagca ttacacaacg
    15361 attagaagtt ggcggacaat atcaatggaa gcgtcgtggt gaagtacaca tgtttaatcc
    15421 tgacacgata catttattac aacatgcttc taagaaaaat gattttggaa tttataaaaa
    15481 ctacgcagag aaaataaata atcaagcaca aaaggccatc accttacgtg gcttattaga
    15541 gtttgatatg tctaaagcaa cagcaataga cattagtgaa gtagaaccag cagaaaatat
    15601 attaaaacgc tttgctacag gtgctatgtc atttggctct atctcacacg aggcacatac
    15661 tacattagcg attgccatga atcgcattgg cggaaaatca aattgtggtg aaggtggtga
    15721 agatgaaatt cgatttgaag taaaagaaaa tggcgactct gagcgctctg caattaaaca
    15781 agtagcttct ggacgtttcg gtgtcacttc ctattatttg gcaaatgctg atgaattaca
    15841 aatcaaatta gcacaaggtg caaaacctgg tgaaggcgga caattgccag gccataaagt
    15901 agatgaatgg attggtcgtg ttcgtcattc cactcctggt gtgggtttga tatcgcctcc
    15961 accacatcat gatatttatt ccattgaaga tttagcacaa ttaatttttg atttaaaaaa
    16021 tgccaatcca aatgcaagaa taaatactaa gctcgttgcg gaagctggcg taggcactgt
    16081 agctgctggt gttgcaaaag caaaatcaga tgttatctta atttctggat atgatggcgg
    16141 aactggtgca tctccaattt catctatcaa acatgctggc ttgccttggg aacttggatt
    16201 agcagaagca caccaaacat tagtgaagaa taatttacgc agtcgtgtcg tattacaaac
    16261 tgacggtatg ttgcgtacag gaaaagatat tgctgtcgct actttacttg gcgcggaaga
    16321 atggggaatt gcaacagctg cattagttgt agaaggttgt atcatgatgc gtaaatgtca
    16381 tttaaatact tgtccggttg gtattgctac acaagatcca attttacgtg ctcgttttac
    16441 tggcgatcca caacacgtta tcaacttctt tacttttata gtacaagatt tacgcgaaat
    16501 catggcaaaa ttaggtttta gaacaatcaa tgaaatggtt ggacaaacac aatttttaaa
    16561 acgtcgtgac aatatcacac attggaaata taaacactta gacttatctc ctattattca
    16621 taaagaagag gcaaatgaaa gtgtaggttt atttaaacaa atgcgacaag ctcatccaac
    16681 tgatgaagca atcttagata aaaaattagt tgctgctgca caaagctcat tagataaaaa
    16741 agagctatct acaggtcagt ttactatcat caatacagac agaacagttg gcgcactttt
    16801 aagctatgaa atatcttgta agtataaaaa agaaggctta ccagaagata ctatacagtt
    16861 taaattcaat ggtagtgcag gacaaagctt tggtgcattt gtaacaggtg gcgtgaaatt
    16921 cgaattagca ggagaagcaa atgattattt tggcaaaggt ttatcaggag gaaaattaat
    16981 cgtatatcct gcttcaattg catcatttaa accaagtgaa aatatcatca tcggaaatgt
    17041 tgcattttat ggtgctacaa gcggagaagc ttatatcaat ggtatggctg gtgagcgttt
    17101 ctgcgttcgt aattcaggcg caaaagcagt agtagaaggc gttggcgatc atggctgtga
    17161 atatatgaca ggtggcttag ctatagtatt aggcaaaaca ggaagaaact ttgctgctgg
    17221 tatgagtggt ggcgttgctt atgtttatga tatcgacaag gatttcccta aaaatgtaaa
    17281 taaagaaatg gtagaattag aaggtttaga tacagaagat atcgacactt taaaattcta
    17341 catagaaaga catttaaaat ataccaacag tgaaattgct aaaaatattt tagacaattg
    17401 ggaaattaca tcaaaatact ttgtgaaagt aatgcctact gattataaaa aagtattatt
    17461 agaaagaaaa aataaaacaa atacagtaaa cgtataaaag ctgtagaata gcaaatgcta
    17521 gtctattctt tgcaagtaca aaatattagc acacgataat aatcgataaa agaaaatggg
    17581 aaaaccaaca ggatttttag aatttacaag agaattacct aaatacaagg cacctgaagt
    17641 gcgtatcaag gactataacg aaatctattt gccttttgca gatgaaaaaa cgcaaaagca
    17701 agcagcacgt tgtatggatt gtggcgttcc tttttgtcat aatggttgtc cactcggaaa
    17761 catcattcca gagtttaatg atgctaccta taatggcgat tggaaattag cagcagaaat
    17821 tctattatcg acaaataatt tcccagaatt tacaggcaga atttgtcctg caccttgtga
    17881 gtctgcttgt gttttaggca tcaaccaacc accagtagcc attgagcaca tcgaaaaatc
    17941 tataattgaa aaagcatttg aattaaatct tatccaacct aaacttcctt ctgagcgtac
    18001 tggtaagaaa gtagcagttg taggtagtgg ccctgctggt atggcggcgg cggctcaact
    18061 aaacaaagct ggacacaccg taactgtgta tgaacgcgct gataaaatag gtggtttatt
    18121 gcgctatgga attcctgatt tcaaattgga aaaagaaatc atagacagaa gaatcgaagt
    18181 aatggaaaaa gaaggtattc aatttgtaac gaatacgaat gttggcgttg atattactgt
    18241 tgaagaatta aataaaaatt ttgatgctgt cgtattatct ggcggctcta cagttcctcg
    18301 cgatttaccg attcaaggta gagcattcaa aggcgtttat cctgcaatgg aatttttatc
    18361 acaacaaaat aagcgagtag caaatattac aacaactgtt gaccatagag gcattgctta
    18421 tgagaatggt gatattttag caacagacaa acacgtaatt gtgattggcg gtggtgacac
    18481 aggttctgac tgtgtaggta catctaaccg acatggtgca aaatctgtga cacaaataga
    18541 acttttggcc atgccgccgc aagaaaggca tgaaagcaca ccgtggccac tttggccgat
    18601 gatgctacgc acctccagct cacatgaaga aggttgcgag cgtcaatggt caattttgac
    18661 aaaagaattt attggaaatg aagcaggtga actaatcggt ttaaaaattg ttgaaataga
    18721 atggaaaacc attgatggca gacctcagtt tgtagaaatt gaaggaacag agcgggtatt
    18781 accttgtgat ttagcactat tagcaatggg atttgtacat ccacaacacg aaggattatt
    18841 ggcaaattta gcggttgcat tggacgaaag aggaaatata aatgccaaca attataaaac
    18901 taacgtggat aaaatattta cagctggcga tatgagaaga ggccaaagtt tagtagtttg
    18961 ggcaatctct gaaggcagag aatgcgcacg tgaggtggat ttatttttga tgggcgaaag
    19021 ccaattagaa gcaaaagaca aaggcgtatt atttgtagga gaataaaaaa ataaacaatg
    19081 aaaaggttac aatcttagat ttcactttaa ttgtatatgc tgctattttt gcgcttgctg
    19141 ttttgcatat agtagcaatg gcccaaccat aaggttgggt ctcttttttg tgtaaaattc
    19201 gattcgaata attatagtat gttttggcaa atgaatctac attcacataa taaagattga
    19261 tataaatcaa caaaataaga aaagtaaaaa aactgcaatt ttgctattga caattacaat
    19321 aaggtttatt aatattgtaa taacataatt accctttatg aaaaccctat taaacatcgc
    19381 agtattgctg ttatgtatta gcagtaactc ttttagccaa aacgacatca gtatttctga
    19441 ttttcttcag cttcgtgcta ataataatta cacagttgaa tcacgattgt ctgctttaaa
    19501 cattcagcct tatgatgaag aaatcatagg tagcggcaga acacagtaca ccttccaagc
    19561 tgaaaataaa agcacacctt tacaatgggt tgattttatc tatgagcaag atgcagcatg
    19621 gaataataga atttcatttc aaacacaaaa cttagcgcaa gtaaaaaaat acttgacaga
    19681 aatgaaatca cttggcttct attttgttgc taaaaaaatt gttgatagac aagcatacga
    19741 agtatattca gatggaagca acacaatcga attaatcaca tcacaaagca gaaaagtata
    19801 tgacaataac ttgtatgtaa atttcgcttt ctacagtgct ggcgaatatg aatatgcatt
    19861 tgcaagcgaa aatgaaaaat acaatgttgc gcaattcaat aaagaagaat tattcgcaga
    19921 cttagtaggc ttaccaataa aataattttt aatccattag tttttcatat tgttagcttt
    19981 aggagttgaa atttcaactc ctattttttt tatggtgctg gattgggaaa tgctaaatgt
    20041 atttcctcta ttgcctgtat cacatcatta gacaactgaa catgaatagc atctatattt
    20101 tctttcagct gttgcattga ggtagcacca ataatattgg acgtaagaaa tggttttgaa
    20161 ttaatgtatg ctaatgccat ttgcgctaat gacaagtgat gttcatttgc taaagctgca
    20221 tactgctcca ctgctttcat tgtatttgga tgagcatagc gcttaaagta atttgcccag
    20281 cgagtaaaac gagcaccatc tggtctattt ccatgtaaat atttttcagt aagcaaacca
    20341 ccacctaaag gcgaataagc caataagcct acatgctcac gaatgcccat ttctgctaat
    20401 ccaatctcat acacacgatt caccagatta tatggatttt gaatcgtaga aatttttggt
    20461 aaattatatt tttctgcgca ttgtatatat tgcatcatgc cccaaggtgt ctcatttgaa
    20521 atgcctatat atcttatttt tccttgttga ataaaatctt gcagcacgct taaagtctcc
    20581 gcaaaatttg tcacagcatc atcagaagta tgtgcgtagc ctaatggtcc aaaatagtta
    20641 gtttgtcttt ctggccaatg caattgatat aaatcgatat aatctgtttg taaacgttgc
    20701 agagattgat gtagcgaatt agttatgttt tttctgtcaa attttattcc agtaccaata
    20761 tgttttaatg cagctgaagg gccagtaacc tttgatgcca aaataatatc cttacgatta
    20821 ttgttgtatt taagccatgt accaataatg cgttctgtag aaccttgtgt ttctgctcta
    20881 gaaggaacgg aatacatttc agccgtatcg ataaaattta tgcctctgtc atgaactgca
    20941 taattaagct gctcatgtgc ttctgcttca gtattttgct cgccccaagt catcgtacct
    21001 aaggcaattt ttgatacttt tattgaggtg ctaggtaatg ttgtataaat cataaatatg
    21061 gaataagatg aataatagta ttaatgaaaa tttgggcaaa atagtttact gtttgacatt
    21121 taattatcat aaaaaaaacc tgccaatttg tgacaggttt ttctttgtat ttggaaaatg
    21181 cattcgcact attcttcact ttttaggctt gctgcaacat attctctgtt taagcgtgca
    21241 atattttcga tagaaatttc ttttggacat tctgcttcgc acgcacctgt gtttgtgcag
    21301 ctaccaaatc cttctttatc catttgtgct accatatcta acacacgtga ttcacgttct
    21361 ggttgtcctt gtggcaacaa tgctaattgt gaaactttag cagacacgaa caacatcgct
    21421 gatgaatttt tacatgctgc cacacacgca ccgcaaccaa tacaagcggc agctgcaaat
    21481 gctttatccg catctgtttt aggaattggt gtagcattag catcaaccgt aacaccagta
    21541 tttacggata tgtagccacc tgcttgaata attctatcaa aagcagctct atcaactact
    21601 aaatctttaa taacaggaaa tgcttgcgct ctccaaggtt caacaacgat agtgtctcca
    21661 tctttgaaag cacgcatgtg caattggcaa gtagtagtgc catgccatgg accatgtgga
    21721 cggccattga tgtacataga gcacatacca caaatacctt cacgacaatc atggtcaaat
    21781 gcaattggct cttgattttc ctcgatgagt tgttcgttca atacatcaaa catttctaag
    21841 aatgacattt cagaagaaat attttttact tgatacgtta ctaaaccacc tttatccttg
    21901 ttgttttttt gtctccacac tttaagtgtg aggttcatat tgtagtgttc catactttat
    21961 tatttataag agcgtgctgc tattttgata ttttcataaa ctaattcttc cttatgtaaa
    22021 ttccaattac tttcgccaac aaattcccaa gcggctacat atttaaaatt cacatcatcg
    22081 cgttctgctt cgccttcttc tgtttgtgat tcttctctga agtggccacc acagctttct
    22141 tttctattta aagcatctct acacattaac tcgcctaatt cgataaaatc agcaacgcgg
    22201 cctgctttat ccaattctgt attgaattct tcgatacttc caggaattct cacatcgctc
    22261 caaaattctt ttttcaatgc ttgaatttct gcaattgctt ctgtcaaacc ttgttcgtta
    22321 cgagccatac cacatttttc ccacataatt ttccctaagc gcttatggaa actttcgaca
    22381 gattttgttc ctttaatatt cattaaagca tttattctgt ctgcaacatt tttttctgct
    22441 gctaaaaatt ctggactatc tgtagatatt gctttagtag cgatttcatc agctaagtaa
    22501 ttaccaatag tataaggaat aacgaaataa ccatcagcta aaccttgcat caaagcagaa
    22561 gctcctaatc ggttggcgcc atggtcagag aaattacatt cacctaaagc atataaacct
    22621 tgtacgctag tcatcaattc ataatcgacc caaagtccac ccattgtata gtgaactgca
    22681 gggtaaatac gcattggcac ttcatatgga ttttcgcctg taattttttc atacatatcg
    22741 aataaattac catattcttc tttcacaact tcttttccta agcgcactaa agttgcttca
    22801 tcaggatttt caatacctaa ttttgaagct tctgctcttc cgtatttatt aattaagttg
    22861 aatctgaaat ctaagtaaac tgcttgttta gttgtaccca cgccatagcc agcatcgcat
    22921 ctttctttag cagcacgaga agccacatca cgaggaacta agttaccaaa tgcaggatat
    22981 cttctttcca aatagtaatc gcgttcttct tctggaatat catttgcttt tctgttgtcg
    23041 tcttttttct taggcaccca aattctacca tcattgcgca aagactctga catcaaggtt
    23101 aattttgact gatggtcacc tgatactgga atacaagttg gatgaatttg cgtaaagcaa
    23161 ggattaccaa aaaatgctcc ttttttatgt gctttccaag aagcagttac attcgagccc
    23221 attgcgttcg ttgataaata gaatacgttg ccataaccgc cagtgcacaa cacaacagca
    23281 tgaccaaaat ggcgttctaa tttaccagaa attaaatctc ttgcaataat accacgtgct
    23341 ttgccatcga tttctacaat gtctaacatt tcgtggcgtg catattgttt tacattacct
    23401 aatgctactt gacgttctaa tgcagagtaa gctcctaata ataattgctg tcctgtttga
    23461 ccagcagcat aaaacgtacg ttttacttgt gtgccaccga atgaacgatt atttaaaaag
    23521 ccaccatatt cacgtgcgaa tggaacacct tgtgctacac attggtcgat gatgtttaca
    23581 gacacttctg ctaagcgatg cacgttagcc tcacgagcac gatagtcgcc accttttatt
    23641 gtatcataga aaagacggta cactgaatcg ccgtcatttt gataattttt tgctgcgttg
    23701 attccgcctt gtgctgcgat agagtgtgca cggcgaggtg aatcttgaaa acaaaaagct
    23761 tttactttgt agcctaactc acctaatgta gcagcagcag atgcgcctgc taatcctgtt
    23821 cctactacaa tgatttcgat tttacgtttg tttgcaggat ttacaagttt tacgtgtcct
    23881 ttataatctt cccatttttt ttctacgggt cctgcaggta tttttgaatt taatgccatt
    23941 gtgaaattgg tctaaataga ttaaaatgat tattgtacaa gtccaaaaaa tattgctaat
    24001 gcaattgcac caaataaacc tggcactacg atagaaaatg ctgttcctaa agctgcaatt
    24061 attggtgtat attttttatg gtttaatcct aaagtttgga aagcacttgg aaagccatgt
    24121 aataaatggt aacctaatgc gatgaagcct aaaatataag gcacaacaat ataccatttt
    24181 gtcaatgctt ctttcacttc taaaaatgaa ttttcttccg cacctgtaaa tatggctact
    24241 ttcgtatgat accaaaagtc gcccatgtgc aatacgagga aaataagcaa taataaacct
    24301 aaaacagaca tggaacggct ataccattta ctattagcat ttcctgcact tacagcatat
    24361 gccactggtc gtttcgcttt attttcgaaa tgtaaacgaa gtgtcaaacc gatatgagca
    24421 agcaagccaa gtaataaacc gatttccatc acgcgtatta tccaattgtg tgacatgaag
    24481 tgagctgctg tgttaaacgt ttcgccatta tcatttatga gtattgtgga attgatgcca
    24541 acgtgtacta ctaaaaatga aatcaaaaag agacctgtta aaccgacaat tatttttttg
    24601 ccgatagagg aagtgataaa tttttctttt gccatgatga tttaatttca aaaaccagcg
    24661 caaaattaac agtttcttgc ttaattatct tttgcttaag attaattttt tgcacatcag
    24721 taatttggaa tttatctaaa tagtaattat ccttgatttt aaagataaaa aaacaccaat
    24781 aaatacgatt gagtatctat tggtgataat attgtaaata gaaaaaatta gtactatata
    24841 tttatgcgta cgctttcagc aattcgctta ctaagtcgat atatttttgc tttgcagcat
    24901 ctttgctcat gccagcttta tctttccaag cattccattt aaactgacct ttgatatcga
    24961 acatacctgg ttttttggtg tcgttatctc cgtctgtagc ctgtttaaat aaagcataca
    25021 aatccaataa ttgtgcatct gttggtcttt ttgtaagtgt tttcacgtcc acttgtgctt
    25081 gtgcaaactg attatctata ctcatttttt tatttttgct aaatttagaa aaaatccttg
    25141 taaataaata aaatgttttg tttgaaagaa ctgtattttt acccttgtta ataaaacata
    25201 tgaaaagact acaatttgcc atactattaa gtattccatt atgttggttt tttttcaagc
    25261 ttttacaagc ttatacgcat ccgtctgcct gttctgttcc aaactttgta tatgctgaaa
    25321 atttagaaca gttaaatcgt tggtgttttg atgcatatag caaaacacgt tatgacgaca
    25381 gtgcgaattt tttatacgtt ttttcacttt ggttattaat ccatttttta aaatacacaa
    25441 gtataaaagc tgcgtttgtc gtgtcaacga tgagcagttt agtatctgta tatttaatac
    25501 aacgaattat agattctaga ttttttggcg tcaacttatt attggtgggt ttattcttta
    25561 tgagcacaca aatttgggct ggcgtattag gcgatgaagt attgtttcaa ggtatgttgt
    25621 tgttattagc agtacgttcc tactggaagc aacgatattt ttggttgatg atttggtctg
    25681 ctttaaatat catgggaaga cctgatacac tctttttagt tttaccttta atcattgcca
    25741 gctacacaga tatcagagaa ttaaaagagc gttatgttca taaatttatt attgcgcgca
    25801 ttaggaaaac tgtattacta cttatagtgc cagtgctttt atttttcact tatcgttact
    25861 tatattttgg aaaaataatg ccctataatt ggttgcatca ttcattagaa acggataagc
    25921 aattcggtat ttttaacttt gaagccttca attatttaaa acattatttg cgtttttata
    25981 ctttgccttt gttcattggt gtaatctttt attttttaaa agaacgaagt gacttagcgc
    26041 tacgatatta tgcgttagcc attggtttaa ttgttattcc aatgatttat gtttgtactt
    26101 attcacaaga cgaaaactta gcctataaaa attattatag catttattta ggtttaatat
    26161 tacttgcctt attgttcata cgcgattatc gttccataac acaaggtgtc gcaactgcaa
    26221 tatttgtatt gttttttgga tttaaaagtt cgtttgagtt tctgcaaaaa acattacaaa
    26281 gttataatga caacgaattt tatatcgcta atgacttatc tcaaattaat aaaggaaaag
    26341 taattgtaga ttacaacacg tttatcagct ggctgaccga gtggagaacg atttatacaa
    26401 atggcagatt tacagcagga gaaaaaatgt taacaaaaga cgaaatatat aatgcaaaag
    26461 cagaccttat cattactgaa aacattacag atactgcatt ttatcaaaaa cattatactc
    26521 ttctacgtgt tcctaaaaac acacgtcaat ttgagcaaca aagaaaacct gataattcat
    26581 tagatcaatt tttttataaa aattcaagaa aagtacaatt gccaaagaac aataattatc
    26641 atgtattggc attgaaagaa agtaaaaact acaacgaaat aataaaaatt ttaaaaagcc
    26701 acggtgccaa tactgtgcaa cttaaaaagt agctcctgcg agaatcgaac ttgcattaac
    26761 agtttaggaa actgctgttc tatccattga actaaggagc cttccgtcgt atgattacca
    26821 tagtaattac tacgataagc ttaacatttt tgatattcta cttaaacatc tatcttatta
    26881 catttcctgc ttgaacacca tcagctttaa cgaagtataa tttgccattc acatcttcag
    26941 ccatcaatat catgccatta ctttctattc ctttcaattt tctaggagcc agatttacca
    27001 acacgcttac ctcttgtcct ataatttcat ctgcttggaa atgcgctgca ataccagaaa
    27061 caatgacgcg ctgctccaca ccaatatcta ccaataactt caagagttta tctgcttttg
    27121 gcactttttc tgcttccaat atcttacctg ttcttatatc taattttgcg aaatcatcgt
    27181 atgtaatttc agatttaatc gaaataaatg atggtgaaat tccagtggaa tcatctgtat
    27241 ttgttggtgc ttgcactatt ttacttttat tcaatttatc gatttgaaga gctacttgct
    27301 catcagtaat tttttcaaac aaaatttgag caggcttgat tttaccatta aatcctgcaa
    27361 attgattttc aattttctgt gctgtattag gtaataatgg cattaatact tctttcagtg
    27421 ttttacaaat gaaaagtgcg ttttgcatta caattttcgt gcgttgttca tctgttttaa
    27481 taagcttcca tggttcatta tccgctaaat atttattgcc taaagaagaa atagacatta
    27541 ctgtagaaag tgcatcttta aacttaaact tcgatataga aacatgaatt gtgtctctca
    27601 aattagttat agcattcagc acttcttctt gctcattcga cgttgttgtt ggaatatcac
    27661 catcataaaa tttatgcatt aatacggcga ctctattgac aaagtttcca atatttgcag
    27721 ctaactcgct attcactcta ttttgaaaat cttgccatgt aaattcagca tctttatttt
    27781 ctggcatatt ggcaattaag caataacgca attcatcttc tttgcctgga agctcctgta
    27841 aatactcatg tagccacact gcccagtttc gagaagttga aattttatca ccttctaaat
    27901 tcataaattc atttgcagga acattatccg gcaggataaa attttcatgt gcttttagaa
    27961 ttgcagg
//
LOCUS       DAOQQV010000025        26607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_546_length_26607_cov_7.913265, whole genome shotgun sequence.
ACCESSION   DAOQQV010000025 DAOQQV010000000
VERSION     DAOQQV010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26607
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_546_length_26607_cov_7.913265"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(528..1199)
                     /locus_tag="PLL59_04370"
     CDS             complement(528..1199)
                     /locus_tag="PLL59_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23072.1"
                     /translation="MRKIIFYLFIAIIFVGGCARPYRKINMSAIPFKEYRQENNISYS
                     LRQGVLYNLKNYYFARREQKKNMSLVAVKIVNKSELPVNINDLQFTCGAALPIQRVGI
                     DEYYNAIKQKSALYWLYAVGFAVYPKPAVYTDPVSGTQVPKADNPKKFIKNGKQIIPL
                     PFGLPVAAANYAIAYKANKKMMLDFKLLDLSNRVIQPFDSIQGILTFKNIANCGDIFI
                     TTNPK"
     gene            complement(1238..1726)
                     /locus_tag="PLL59_04375"
     CDS             complement(1238..1726)
                     /locus_tag="PLL59_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23073.1"
                     /translation="MLTPYHLLPDNAKVWIFQANRSLTQDEVWEISDILENFTDRWQS
                     HGRDIAAFGSLYYRRFVVLMADESAFTLSGCSIDIATKLMKELESAYDLQFFDRMKLC
                     YKVTNDLVGSFSLSQLAENIATGKIKKDTIVFNNLVDTKLDFETKWEQALQLTPYAKF
                     LD"
     gene            complement(1790..2575)
                     /locus_tag="PLL59_04380"
     CDS             complement(1790..2575)
                     /locus_tag="PLL59_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMY23074.1"
                     /translation="MQYKVPVVSELVAEGKTPDVIFWIGCAGSFDQRAQRVTIAFTKI
                     LNQLNINFAVLGTEEACTGDPARRAGNEFVFQMLAQQNIATLNGYGIKKIITTCPHCF
                     NTIKNEYPALGGLYEVVHHSTYLQQLINDGQLKVEGGTFKGKRITYHDSCYLGRANEI
                     YEAPRAVIEALDADLVEINKSKKNGLCCGAGGAQMFKEDEPGNKRINVERTEQLTEVY
                     PDVIAVACPFCNTMITDGVKGENKQDDIKVLDIAEMVAQANNY"
     gene            complement(2616..3947)
                     /locus_tag="PLL59_04385"
     CDS             complement(2616..3947)
                     /locus_tag="PLL59_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMY23075.1"
                     /translation="MLFLKIFQILLFIGLVGGTFYFAFKKFKEIYDNIMMGQAEKINS
                     TLGFRIKSMLLIAFGQKKMFKLTFAALLHLVIYVSFCITQIELLEIFIDGLTGSHRII
                     YHAVENIPFLHTLYLVVINFIEILSLLTFFVTIIFILRRTTFKVERFQKPELKGWPWK
                     DALLILAFELTLITFIFLMNGADSALHIKEHGEGYGFFLSSAIGGMLQALPSGLLQAL
                     ERIGWWGHLCMVMGFILYLPYSKHLHILLAFPNTFFTRPEPKGEIHNMPAIQHEVQSM
                     IDPSFVVPASDGEVKFGAKDVYDLSWKNLLDAYTCTECGRCTAACPANQTGKKLSPRK
                     IMMDTRDRMEEIAACKKEHGPEFVDNKSLLGDYITGEELRACTTCNACVEECPVNISP
                     LSIIMELRRNLIMEASDSPAEWNNIFANLENNQAPWQFSPNDRLNWTEELN"
     gene            4062..4580
                     /locus_tag="PLL59_04390"
     CDS             4062..4580
                     /locus_tag="PLL59_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMY23076.1"
                     /translation="MLIPSSAQKILDKQKIELKLDRMAFQALELTYDNDDLVILGIAP
                     GGGLTLAKIIADKITAYQSNKKVEFSSIVINKTSPLAEEVYLSDDLDLNNKTILLIDD
                     VGNTGRTLFYALKPLTAFYPKKIIIGILVDRTHKTFPIKADVVGFPIATTLNEHIIVK
                     MENNLPEGAYLF"
     gene            4605..5693
                     /locus_tag="PLL59_04395"
     CDS             4605..5693
                     /locus_tag="PLL59_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23077.1"
                     /translation="MKSVIQFFVLIFLLLNSICTEAQILQPTSEEKIIIERFEYIRQI
                     KKYVGNLFWKNFSNNEFPGTVVYFTDSASYFINPLPEMKDKVSKYAIVENDYDWNIWK
                     LRMPLDSAKFVMETQFQFDPKAKAYINYRTPVLFCSSPELTRKEKVVINTTQEWAIAL
                     MHELYHQYQYSNEAILTYVLRLYQEKKMIDMDSLQTIYLSNQAFRDTLKLENEILKKA
                     VAATSFDEEKKLFTQFLKIRAKRGVDFFKKKKFYISTPENFWEKLEGTSLMMEHELKQ
                     HISEIKPTPYLLENDPMYENNFKFDESSATEIDFYTELNDERFYVGATGFNMVRLLEK
                     NKVPYKDNFFSYASLPLELQLKYFYKIK"
     gene            5703..6371
                     /locus_tag="PLL59_04400"
     CDS             5703..6371
                     /locus_tag="PLL59_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HMY23078.1"
                     /translation="MTIDLSRVFSKKLLLKFYLPLASVITFFVFYISHIVEKNAKDKL
                     YTSVSEIPYNRVGLLLGTCKTMDDKVTINPFWKFRVDATYALWKAKKIDRILISGDNG
                     WHGYNEPQDFLDTFIALGIPDSCMVCDFAGFRTHDSVIRSKKVFGQNKVTIISQKFHN
                     ERALFIAQKYGIDALAFNANDVSFRSGLYTFFREKAARVLLFLDLYVLNTQPHFIGKK
                     IKIN"
     gene            complement(6368..6871)
                     /locus_tag="PLL59_04405"
     CDS             complement(6368..6871)
                     /locus_tag="PLL59_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HMY23079.1"
                     /translation="MLIFLVGFMGCGKSYVGRHLAPLLQYDYIDLDKFIEDNEGMSIK
                     EIFEQKGEAYFRNLERNFINNIDAQQNIVISTGGGAPCFFDNMEIMNTKGITIYLNRS
                     KKTTLERLHKGKHKRPLIADLSDEELSDFYDERLAGRKPFYDKAQLHVGDADVEIILA
                     MIREKIN"
     gene            7123..8367
                     /locus_tag="PLL59_04410"
     CDS             7123..8367
                     /locus_tag="PLL59_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HMY23080.1"
                     /translation="MEAKKVIGQTFTFQNGNTVKNRFLKSAMSEGLGDKNGAPTTALN
                     TLYGTWADGGIGICVTGNVMIDKRALGEPGNVIIEDEKHLPQLKAWAKAATRNNTHCW
                     VQLNHVGKQSPRGLNKENVAPSAVPFRTDMRPYFATPRELTDNEILDIIQRFGNAARI
                     VKKAGFSGVQIHGAHGYLVSQFLSPHHNIRNDRWGGNPENRRRFVLEVYHAMRAQVGP
                     DFPVSIKLNSADFQRGGFSEDESLDTIRALADAGIDLLEISGGNYEEPAMTGIAIKES
                     TKMREAYFMDFAEKARKVCTIPIAVTGGFRTAEGMANAISSNAVDFVGIARHLAIETD
                     VPNKLLSGKLPKHSVKPIKTGIPMIDNMGIMEVSWYSAQLKRIGNGKKPNVDASPFLE
                     FLKSIFHQIGITKRLPSRLRAK"
     gene            complement(8457..9518)
                     /locus_tag="PLL59_04415"
     CDS             complement(8457..9518)
                     /locus_tag="PLL59_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMY23081.1"
                     /translation="MSTFIFNKLFYGLLVLLGVVFVIFGLFNILPVDPARLTLGQRAD
                     IESVEAINKEFGLDKPKYVQLALYLNDLSPIAVHENTKEAKEKYNYTKLFAVSKEKAL
                     VLKMPYLRRSYQTKENVSEILARTIPTTVLLAITSILLAIIIGIPLGVIGALKQHSWF
                     DNIAMSISVLWISAPSFVTAMVLSLYFGYKWHDFTGLEVSGPLFWINDYGEEVLLLKN
                     LILPTIALGTRPVAIIYQLTRSSMLDALGYDYVRTARAKGLSEATIIIKHTLRNALNP
                     VITSISGWFASLLAGAYFVEIVFNLDGLGSKTVQSLNTFDFPVVMGSILFAATVFIIM
                     NFLVDMLYAILDPRIRIGK"
     gene            complement(9537..10772)
                     /locus_tag="PLL59_04420"
     CDS             complement(9537..10772)
                     /locus_tag="PLL59_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23082.1"
                     /translation="MDLITLIGMFAAIAVVFTLIGKFLFKQSNLPINFLRYFVGVWFV
                     FSGVVKAIDPAGTAIKMEEYFEIFEKSAPLLSFLWKLMAEHALAVSFVMIIFEIYLGI
                     ALILGTWKKVTIWLLILLIAFFTFLTGYSAKTGKVTDCGCFGDFLKLAPIQSFYKDVF
                     LCVLILIILFGQKSIKEIFGKIINRGLLLLLTIASIAFAYRNIYFEPIKDFRPYQVGS
                     SIPEGLKLPANAKPYKYENTFIYKNKKTGEVKEFKGTWPQDIDNWDFVDRKDVMIQKG
                     DDPKLKDFAIKDANGEDYTSVFFEEEEYLFFIIIPELKKVNEDGYIKLLKVTDAAADD
                     GKLVYILTGSLIDDMKAFEEKHNKEYDYEIYNTDATPLKTIMRSNPGLLILKKGIIVA
                     KYHFNELTTYADLKKSVLK"
     gene            complement(10823..11371)
                     /locus_tag="PLL59_04425"
     CDS             complement(10823..11371)
                     /locus_tag="PLL59_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HMY23083.1"
                     /translation="MNENTITQYENAFASCKDVFLKKTKDYGTSWRILRARSITDQIY
                     IKAKRIRTIDETGENKVGDSIESEFIGCVNYGIIAIIQLQLEHKKDYAIELKYEEAEV
                     LFDEQYSIIKKLFLAKNHDYGEAWRDMRISSFTDLILSKLHRIKQIEENKGQTIISEG
                     IDANYMDIVNYAIFALIKLNEK"
     gene            11440..12276
                     /gene="folP"
                     /locus_tag="PLL59_04430"
     CDS             11440..12276
                     /gene="folP"
                     /locus_tag="PLL59_04430"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMY23084.1"
                     /translation="MKKFTLNCKGKLFVAAQPLIMGILNCTPDSFYDGGKFPSIDTLL
                     QQTEKMLQEGADIIDIGAVSSKPNSVQVSAEVEINRIIPIVEAVHRHFPEALISIDTF
                     RSDVAEICIQHGATIINDISGGHADENIMRVAAKYHCPYIMMHRLGDFETMHQQFEYT
                     DVITSVFDYFSAKIETAKKFGINDVIIDVGFGFSKTIPQNYTLLKNLSIFEQLNKPIL
                     VGLSRKSMLYKLLNTNPENALNATTAVNSIALQQGASILRVHDVKEAAECVKIYNQLQ
                     AI"
     gene            12273..13175
                     /locus_tag="PLL59_04435"
     CDS             12273..13175
                     /locus_tag="PLL59_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23085.1"
                     /translation="MKSVLTYLLLFLFVNSLQANDAIEGKWRGTIVGHVDDRDYFIHA
                     EIAAKKREGEFTIKMKLFSEDYTGEFLLQTEMIAEKLYIRKLEKIAEFPYPYLHIEDC
                     FTGYFLLKKSANNKMELDLYRNPVYHKIEEFTNLDGDGNFSPGFQCFTTVILRSKHSD
                     TTFNAKEKRSDSLVTAKKIRADEVLKRKQVSTKTWTVKKNKIVLQVWDNNKEDGDIIS
                     LKFNDKWILTDFLLKKEKHTIPLTLNKKENQLLLFAENLGSITPNTAAVSIDDNVLVR
                     TFILNSDMNKSETIKISLADTKVK"
     gene            complement(13177..14337)
                     /locus_tag="PLL59_04440"
     CDS             complement(13177..14337)
                     /locus_tag="PLL59_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMY23086.1"
                     /translation="MKNIFILIFVCMSTLTFAEDTGMQFENGASWQQILDKAKKENKF
                     IFLDAYASWCGPCKWMAKEVFPKPEVGEALNPNYVNAKIDMEKGEGIELAKKYNVRSY
                     PTYLFFDANGELVHRSLGSMPAADFITLCNNTLDPEMQFITLKKKYESGVRDTAFLRN
                     FVENANNAQDESAQPALKDYLQQSNYNLSPDNIRLISLLTNSIHDTGYAILQDNKTLF
                     YETLGIKNVDNQIEELVWIEAKKASKKGTDKEAFKKIIQQYLPEKTEILCAEFELSLL
                     KRAANWKAYLPKALAFADKFCKNDFERLNSIAGTLWENYKDKTTLGKALQFALRSVEL
                     NSNFDNNDTVARLYQKLNNKSSAKKYAEISLALAQKEGIDTFSIEELLKALK"
     gene            complement(14374..14769)
                     /locus_tag="PLL59_04445"
     CDS             complement(14374..14769)
                     /locus_tag="PLL59_04445"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMY23087.1"
                     /translation="MTFEQRAALSETHIFKAVFPNTTNHYDTLFGGTAMQLMDEVAFI
                     TATRFARLKMVTVSSDKIDFNVPIPSGTIIELVGRVVKMGNSSLDIKVEIFIELMNSD
                     KRELAINGMFKFVAVGENKKPIKIDLDLI"
     gene            14977..15336
                     /locus_tag="PLL59_04450"
     CDS             14977..15336
                     /locus_tag="PLL59_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23088.1"
                     /translation="MKVIHFEIPAKNPERLKHFYSKIFDWRFCQWKEAGFWYALTGSP
                     SEFGIDGGIIDVKELDIPVNSMIAVENIDTTLHKIVEVGGKVAVPKFYEQGVGYLAYF
                     NDLDGNIFGLREVNPNV"
     gene            15346..15711
                     /locus_tag="PLL59_04455"
     CDS             15346..15711
                     /locus_tag="PLL59_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMY23089.1"
                     /translation="MNRVVNFDFPTDNPENAKHFFSEIFGWKFTEWLNEEYWLIETGD
                     KHKQGINGSMIRRRHEQQMSSIMVQVENIDLVLSRIQAEGGEVVFPKQVVPKVGYIAY
                     FKDPENNVICIMEFDVSAK"
     gene            complement(15712..16599)
                     /locus_tag="PLL59_04460"
     CDS             complement(15712..16599)
                     /locus_tag="PLL59_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMY23090.1"
                     /translation="MQLRSKAYTQMHIATFLWGLTAILGKLISIGEFSLVWYRVLFVS
                     IAMLFIPKIIKHVRQLDKKTVLQISGIGILLSIHWVAWYASIKYANASVAVSCIACVS
                     LFTAILEPIFTKTPFKTSNLLLSIFVIPGILLINQSLDFHYRFGFFLGIVAAFIAAIF
                     GILNKKYTQDVQANVITFIQMLSGFIFLTLLMPLYLYFSKNEFPIPHQTDVFLLLVLA
                     LFCTVIPYNLFLKTLKITDAFTTSLINNLEPVYGIILAIIFLGENKELNWQFYCGTLI
                     ILFAVFLHAFLTQIKNRKM"
     gene            16698..17042
                     /locus_tag="PLL59_04465"
     CDS             16698..17042
                     /locus_tag="PLL59_04465"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HMY23091.1"
                     /translation="MDKKYQTFWSFYPYYLTEHSNPICRITHYIGTSLLFVVIALSIY
                     TSNAKLLFLLPLVGYGFAWLGHFVFEKNKPATFQYPLYSLGSDFVMFYHFLTGQVNQK
                     VEDAKKTVASQS"
     gene            17144..18652
                     /locus_tag="PLL59_04470"
     CDS             17144..18652
                     /locus_tag="PLL59_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMY23092.1"
                     /translation="MNKNLILFTSILLFLGLFGIGIVQIFWLKGAIKSREQDFDKAVF
                     EAMNDMSIKIEDLSYKPILSKMLQSQKIHTNEYGEVIIEMSDDNADYESAYAEPPHGE
                     FPSNGKHKKNKAIQPNALLPMYEDANGNADNSFPLEIDNLQQFMAQQMTSLQPITEIL
                     DTTKLKEIITAELNSHGIKTKVKYGVTEYAPNNFVLLSKQAPLAELFRTPYSVDLFRR
                     SMFDDNKKLKLIFPDKKKYLYSTMSPMILSSSVFFLMVVAAFFLSFQIIFRQKKLSDM
                     KTDFINNMTHELKTPIATISIASEMLKDNSISASQENRAKYAGIIFDENKRLYNHVEQ
                     VLQIARLEKGELQLNFEDRDVHAIITATANRFNLRLEELQGSIALQLNAEQSIFKIDE
                     MHFTNVINNLIDNAIKYNDKAPLIKINTFNIITGIQIEVIDNGVGLAKEDQQKVFEKF
                     YRVTKGNVHDTKGFGLGLSYVQSIIDKHSGKVWVESKIKEGSTFIIQLPSNV"
     gene            18672..19385
                     /locus_tag="PLL59_04475"
     CDS             18672..19385
                     /locus_tag="PLL59_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMY23093.1"
                     /translation="MTTTAIKKKVLLVEDDPNLGMLLQEYLRHKNFEVELKRDGVEGL
                     FAFRKNGYDIILLDVMMPKKDGFTVAEEIRKEDKDVPIIFLTAKSLKEDKIKGLILGA
                     DDYITKPFSMEILELKMNAILRRTEKHEVQVALDEYRAGDCVLDYKNHKLHVKGQEIK
                     LTTKENELLRLFFEKKNQILERELALKHVWQDDSYFTARSMDVFISKIRKYIKDDERL
                     QILNIHGQGYKLVENEAVA"
     gene            19465..20001
                     /gene="dcd"
                     /locus_tag="PLL59_04480"
     CDS             19465..20001
                     /gene="dcd"
                     /locus_tag="PLL59_04480"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HMY23094.1"
                     /translation="MILTDKGILAEMEKGTIIVEPFDREYLGTNSYDVHLSKHLATYT
                     QPVLDAKMHNTIHHFDIPEEGFLLEPGILYLGATLEYTETHAHVPFLEGKSSMGRLGI
                     DIHATAGKGDVGFCNHWTLEISCIQPVRIYAGMPVGQLIYFVVDGEIENAYNKKVSAK
                     YNQRTAKPMESMMWKNFK"
     gene            20068..21114
                     /locus_tag="PLL59_04485"
     CDS             20068..21114
                     /locus_tag="PLL59_04485"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HMY23095.1"
                     /translation="MSPLNKEQLLVDLKNWNQIIRKYQVPSTKEALIQIANSFSFYLI
                     LLALQFYLYDKSFFWSALVALLNGFMLSRIFIIQHDCGHSSFTKYKTFNEIIGTVCSV
                     CTLIPYRYWARNHSFHHAHNGQLEVSDIGDIEVFTTEQYAKANWRQKLYYRIYRNPFY
                     LFTIGGFIYVVIYNRFAFVNSDYFKKVKQSVNISNILFIVVYALCAYLFGVERFLVVQ
                     FTNLFFFGIYALWFFYIQHQYEHIFKAKKEEWNYVVAAIKGSTYYKLPRVFHWLSGNI
                     GYHHIHHLSPAIPNYNLRKCHNENPVFLKYTNTLTFWQSLKTLRSNLWDEQQKKMVSF
                     GEYKRNRRLKSMMK"
     gene            complement(21260..22963)
                     /locus_tag="PLL59_04490"
     CDS             complement(21260..22963)
                     /locus_tag="PLL59_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HMY23096.1"
                     /translation="MEITETEENIDLHPQDQDANRQFEQYIEKKPSSLFVRFLITNRH
                     LSGLLLGGGYAYLRHRRETKAKFSIGIDIFLRLFLWLHWPILKKNLIKQPFAVQLRRR
                     LEMLGATYIKLGQIMSLREDMLPKDITDELKKLLDTLPAIGYDRFAELVEEQIGQPLH
                     RVFTEVKQNPLGSASIAQTHRATLRTGEEVVLKLIKPGTRELIQTDSKIIKVIGAFLE
                     IFIPHLQPKNMFTEFCDYTNREVDFRLEADNAEEFSANFAKHPEIHFPRIFREYSTSD
                     LICMQFIRGIKPDHRAYERYSEADREKIVDYGAYAIIQMLYHDGFFHADLHPGNLMIM
                     DGPNGPQVGFIDLGMVGRFEDTTRKTMLYYFNSLVTGDPQGAARYLTLLARKGKGSDV
                     EGFRKEFVSIGNKWLKAPNFKDFSLAKLILESVALGAKYRMYYPLELVLMVKAIITYE
                     GVGNVILPGFDIQKVTKKHIRKMILGEVNPVDLLKSQLQNAPEIMDIIMRSPLVISEA
                     FKQYETKLVEKKKPISFGLQNTIFGGCCIIGGSILAAAGLEWFVFTPLLVFGFIMALR
                     N"
     gene            complement(23091..23657)
                     /locus_tag="PLL59_04495"
     CDS             complement(23091..23657)
                     /locus_tag="PLL59_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY23097.1"
                     /translation="MKAKFLEICAIPEHALEELFSYFTLETYKKNTHIIRETEPILHI
                     YFIFKGIIRIYFYKNDKLIIERFVKEGGFFGGNYDHLSKYPGVHNYESLEDLVVLKIK
                     HEDLENLYKKHPSIESLYRRQLEVFHFAYVDKLYTLKAANSEERYRDFELEYGDIINR
                     ISMKNVASYLNMTAETVSRIRAKQSGNK"
     gene            complement(23740..25467)
                     /locus_tag="PLL59_04500"
     CDS             complement(23740..25467)
                     /locus_tag="PLL59_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMY23098.1"
                     /translation="MKRIIFFGLLTIFTTKLIVAQDPHFSQIQYNPLYLNPALAGVTE
                     YGKANRLAGNYRDQWRTLPVPYSTTTASYDRLLKKFPKGWLIGGGVSFLYDKAGDGNL
                     SIFNPNISMNFGKYFNKEKQLLTLGITAGITIKSLDELGLQFDNQYNGSTFDPNLPSG
                     EDFSNNNVSYPNFTLGLNFRTKIKEKAVLDIGAATSNLHQPNQNFLYYTSSKLPARHA
                     AYAKAKVNIKDNWNIQPGVFFQNQRKANQVLINAIAEVRFNEKKNFGLGFGAGYRAIG
                     NDAAIAYLSFLYKTLRVGAAYDFNVSGLRKASKAQGAFELALTYEFGEVKTKQCPKCP
                     SYVPANVETIVRVKYEDADNNLVDTIINRIVPADSVPRQYIDSIELILKPIAIKAPAP
                     SVTVVKTTEKIMSMLPIELYFDNDMPKQNSKVDYGELYNSYTARKATFESKSVDGAEN
                     VGTFFSQIDKNFQSVSSVVQALSGLVDSGYVITLEFRGYTSPLASESYNKKLSERRIN
                     SIIAYFDSKLGEQFKILSKTALAIPFGETLSPKEISDNGKDRAKSEFGVAASKQRKVE
                     IVGIRIEKP"
     gene            complement(25547..>26607)
                     /locus_tag="PLL59_04505"
     CDS             complement(25547..>26607)
                     /locus_tag="PLL59_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY23099.1"
                     /translation="NYTIPLISAKGCDSTVFLDLTETDTIIENIEKNICLGQHYSIST
                     QTFNETGNYTVYLTANAGCDSVVNLSLHVLDTLKETINRTICKGDSIAIGTQIFKTSG
                     NFSIPLLAVSGCDSLVNLQLEVIENPITPLRGEICAGEQFTIGNQTLSENGEYDITFT
                     AANTCDSIVHLTLIVHQLPIIDATADKTTALSTEPIQLNVITNENLTYNWSPAQLLEV
                     STLQNPIAHIQSSTWFIVSAQNKITQCIALDSVFIALEYLPCVKENIYIPNAFSPNGD
                     GVNDILFVRSSALKNMHLFIYNRWGLKVFETDDISKGWDGTYNGQPSMEDAYGYYLKG
                     ECLQGDEIILKGNITILR"
BASE COUNT         8748 a         4407 c         4550 g         8902 t
ORIGIN      
        1 ttaccaaata atttgccact attgccaagc tattgttagt ggttcgtggt aatgttttct
       61 tttttttatt aaaagtatat cagcaaattt aagcatgata aaaagcgcat ttgttgacat
      121 ttcgttttct atgtgtcaaa ccattcgtgt ttactactgt tttctggctg gtgaaaatcg
      181 catttgcttt cattttgtct gctgtccgtt tttaacacaa attctgcgaa ttgttttcaa
      241 accattttga gcgcaatttt gtgttgcgtc gtcgaagttg ctgcacgagc caacagctac
      301 caacttagca acttgctaca aacatttttg tcataggtca gcaaacattc attttgaaat
      361 ttcagcgaga aataagtcga agtgaaaact tttttttaaa agtaaaccga actttcacca
      421 cctttcctac catgaccact aacttataat tatgcgactt tgagtttgtc ttttaaagaa
      481 taaaatacag gcaaaaaaca cttgatgcta tgagttagca cgtaaaatta tttcgggttt
      541 gttgttataa aaatatcacc gcagttggca atgtttttaa acgtaagtat gccttgtatg
      601 ctgtcaaatg gttgtatgac tctgttcgat aaatcgagca atttaaaatc caacatcatt
      661 ttcttatttg ctttataagc tatcgcataa ttggcagctg ccactggcaa gccaaacggc
      721 aatggaataa tttgttttcc gttttttatg aatttctttg gattgtcagc cttcggcact
      781 tgtgtgccgc taactggatc tgtataaaca gctggcttag gatatactgc aaatccaact
      841 gcatacagcc aatacaatgc tgatttttgt ttaatagcat tatagtattc atcgatgcct
      901 acacgctgaa taggtaaggc tgcgccgcaa gtaaactgca aatcattaat gtttacaggt
      961 aattctgatt tgttaacgat tttcaccgca accaagctca tatttttctt ctgctctcta
     1021 cgtgcaaagt agtagttttt taaattatac aatacacctt gacgcaatga ataactaata
     1081 ttattctctt gtcgatattc tttaaatgga atagctgaca tatttatttt gcgataagga
     1141 cgcgcgcaac caccaacaaa tatgattgca ataaaaagat agaaaattat ttttcgcata
     1201 gcgaatactt tctgtaaaag taagaaaaaa atatcgacta atctaaaaat ttggcgtaag
     1261 gtgtaagctg taatgcttgt tcccatttgg tttcgaaatc taactttgtg tcaacaagat
     1321 tattgaatac aatggtgtct ttttttattt tacctgtagc aatgttttct gcaagctgtg
     1381 ataatgaaaa actgcctact aagtcattcg tgactttata gcacaatttc attctgtcga
     1441 agaattgtaa atcataagca ctctctaatt ctttcatcag cttcgtagca atgtcaatag
     1501 agcaaccact caatgtaaaa gcactttcat cagccatcaa taccacaaat cgtctgtaat
     1561 ataaagagcc aaaagcggca atgtctctgc catgtgattg ccatctgtct gtaaaatttt
     1621 ccaagatatc actgatttcc caaacctcat cttgtgtaag gcttcgattg gcttggaaaa
     1681 tccaaacttt ggcattgtct ggcaataaat gataaggagt tagcatatag atttattatg
     1741 catcatgaac atgaatgaaa gcttgataaa aattaaagtg caaatgcaat taatagttat
     1801 ttgcttgtgc taccatttct gcaatgtcta atactttaat gtcgtcttgt ttattttcgc
     1861 ccttaacgcc atcagtaatc atggtattac agaaaggaca agccactgca atcacatcag
     1921 gatatacttc tgttaattgc tccgtgcgtt ctacgttgat gcgtttgttg cctggctcat
     1981 cttctttaaa catttgcgca ccacctgcac cacagcacaa gccgtttttc ttcgatttat
     2041 ttatttctac taaatcagca tctaatgctt caataacggc gcgtggtgct tcatatattt
     2101 cattagctct gcctaaataa catgagtcat gataagtaat acgcttgcct ttaaacgttc
     2161 caccttctac tttcagctga ccatcgttga tgagttgttg taaatatgtt gagtgatgta
     2221 ctacttcata cagaccgcct aaagcagggt attcattttt gatggtattg aaacaatgtg
     2281 ggcaagtcgt aatgattttt ttgatgccat agccatttaa tgtggcaatg ttttgttgtg
     2341 ccaacatttg aaaaacaaac tcatttccag cacgtcttgc tggatcgcca gtgcaagctt
     2401 cttcagtgcc gagcacggcg aaattgatat tgagctggtt taatattttt gtgaaagcaa
     2461 tggttactcg ttgtgcacgt tggtcgaaac tgccggcaca gccaatccaa aaaataacat
     2521 caggtgtttt gccttcggct actaattctg aaactacagg tactttatat tgcatttttt
     2581 ttatttgata atttttgaaa aattagaatg ttgtattaat tgagttcttc tgtccagttt
     2641 aagcggtcat taggtgaaaa ctgccacggt gcttggttgt tttccaagtt ggcaaaaata
     2701 ttattccact cagcaggtga gtcagatgct tccatgatta aatttcttct taattccatg
     2761 atgatgctta atggagaaat attaacagga cattcttcta cacacgcatt acatgttgta
     2821 caagcacgta attcttcgcc agtgatataa tcacctaata aacttttatt gtccacaaat
     2881 tcaggtccgt gttctttttt gcaagcggca atttcttcca tacggtcacg cgtgtccatc
     2941 attattttac gaggcgatag ttttttgcca gtttggttag caggacaggc agcagtacat
     3001 cttccacatt ctgtacaagt atatgcatct aataaatttt tccatgataa atcatataca
     3061 tctttcgcac caaattttac ttcaccatca gatgctggca caacaaagct agggtcaatc
     3121 attgattgta cttcatgctg tatagcaggc atattatgaa tttcaccttt aggctcaggt
     3181 cttgtaaaaa aggtattcgg aaatgccaat aatatatgta gatgtttgga ataaggtaaa
     3241 tataaaataa aacccataac catacataag tgtccccacc aaccaatgcg ctctaatgct
     3301 tgtaataatc ctgatggtaa tgcttgcaac attccaccaa tagcactgct taagaaaaag
     3361 ccataacctt caccatgttc tttgatatgt aatgcactgt ctgcgccatt cattaaaaat
     3421 ataaaggtga tgagcgttag ctcaaaagct aaaattaata aagcatcttt ccaaggccaa
     3481 ccttttaatt ctggtttttg aaaacgctct actttaaatg ttgttcttct taaaataaag
     3541 atgatggtta caaagaatgt tagcaaggaa agaatttcaa taaaatttat aacgactaga
     3601 tagagtgtat gcaaaaatgg aatattttct acagcgtgat aaatgatacg gtgtgagcca
     3661 gtcaatccat cgataaatat ttctaagagt tcaatttgag taatacaaaa tgatacataa
     3721 atcactaagt gcaataatgc agcaaatgtc agcttaaaca tctttttctg accaaatgca
     3781 atgagcaaca tgcttttgat gcggaaacca agtgtagagt ttattttctc tgcttgaccc
     3841 atcatgatat tgtcataaat ttctttaaat ttcttgaagg caaagtagaa tgttccgcca
     3901 actaagccga taaataacag gatttgaaag atttttaaaa ataacatcag taatttattt
     3961 gaattaattg cgaaaataca gcattatcta cttattttta gttccttatt tgaaagaatg
     4021 attaaatata acttatttgg aataaatcta aacagatttt tatgttaata ccatcgagtg
     4081 ctcagaaaat attagataaa caaaaaatag aattaaagct ggacagaatg gcgtttcaag
     4141 ctttagaatt gacgtatgat aacgatgatt tggtgatatt aggcattgca ccaggcggtg
     4201 gcttaacatt ggcaaaaatt attgcagata agattacagc ttaccaatcg aataaaaagg
     4261 tagaattttc ttctatcgta ataaataaaa cgtcaccact tgctgaagag gtctatttgt
     4321 ctgatgattt agatttgaat aataaaacga tactattgat tgacgacgta ggaaatactg
     4381 gcagaacttt gttttatgca ctgaagccat tgacagcatt ttatccaaaa aaaataatta
     4441 ttggcattct tgttgataga acacataaaa cgtttcccat taaagcagat gtcgttggat
     4501 ttccaattgc gacaacttta aatgaacata ttattgtaaa aatggaaaat aatttgccag
     4561 aaggtgcata tttgttttaa attagaatta taaaaactat aattatgaaa tctgttattc
     4621 aattttttgt cttaattttc ttattgctta atagtatttg tactgaagca caaatactgc
     4681 aaccaacttc agaagaaaaa attattattg aaagatttga gtatatccgt caaatcaaaa
     4741 aatacgtagg caatttattt tggaaaaatt tttcaaataa tgaattccca ggcaccgttg
     4801 tatatttcac tgattcagca tcctacttta taaatccatt accggaaatg aaagataaag
     4861 tgagtaaata tgcaattgtt gaaaatgatt atgattggaa tatttggaaa ttgcgtatgc
     4921 cattagatag cgcaaaattt gtaatggaaa ctcaatttca atttgaccca aaagcaaaag
     4981 catatattaa ttacagaacg ccagttttgt tttgcagttc gccagaatta acgcgaaaag
     5041 aaaaagtagt aataaacacc acacaagaat gggcaattgc attgatgcat gaattgtatc
     5101 atcaatatca atattccaat gaggcaattc ttacctacgt actacgcttg tatcaagaaa
     5161 aaaagatgat agatatggac agcttgcaaa ctatttattt gtcgaatcaa gcttttagag
     5221 atacactgaa attagagaat gagattttga aaaaagcagt tgctgctaca tcttttgacg
     5281 aagagaaaaa attgttcact cagtttctaa aaatacgcgc aaaacgtggt gttgatttct
     5341 ttaaaaagaa aaaattctac atcagtacgc cagagaattt ttgggaaaaa ttagaaggca
     5401 ccagcttgat gatggaacat gaactgaaac aacatattag tgaaataaaa ccaacaccat
     5461 atctattaga gaacgatccg atgtatgaaa ataattttaa gtttgacgaa agcagtgcaa
     5521 cagaaatcga tttttataca gagcttaacg atgaacgctt ttatgttggt gcaacaggtt
     5581 ttaacatggt gagattgtta gagaaaaata aggtgccata taaagataat ttctttagct
     5641 acgcatcact accattagaa ttacagctga aatattttta taagataaaa tagctgtata
     5701 taatgactat cgatttaagt cgtgtatttt ctaagaaatt actattgaaa ttttatttgc
     5761 cattggcaag tgtcatcact ttttttgtgt tttatatttc tcatattgtc gaaaaaaatg
     5821 caaaggataa attatatact tccgtttcag aaataccata taatagagta ggtttgctat
     5881 tgggaacttg taaaacaatg gacgacaaag ttacgattaa tccattttgg aagtttagag
     5941 tagatgcaac gtatgcgctt tggaaagcta aaaaaattga tcgtatttta ataagtggcg
     6001 ataatggctg gcatggctac aatgaacctc aagatttttt agatactttt attgcattag
     6061 gcattcctga ctcatgcatg gtttgcgact tcgcaggatt tagaacacat gactctgtca
     6121 ttcgaagtaa aaaagttttt gggcaaaata aagtgaccat catttcacag aaattccaca
     6181 atgaacgtgc cttgtttatt gcacaaaaat atggcattga tgcgcttgct tttaatgcaa
     6241 atgatgtcag ttttagaagt ggcttatata cttttttcag agaaaaagca gctcgcgtat
     6301 tgttgttttt agatttgtat gttcttaata cacagccaca ttttattggc aagaaaatta
     6361 aaattaatta atttattttt tctcgtatca tagcaagaat tatctcgacg tcagcatcgc
     6421 caacatgtaa ttgggctttg tcataaaatg gttttctacc agctaatcgc tcatcgtaaa
     6481 aatcggataa ttcctcatca gacaaatcag caattagtgg tcgtttgtgc tttcctttgt
     6541 gtaagcgttc aagcgtagtt tttttacttc tattcaaata tatggtgatt cctttggtat
     6601 tcattatttc catattgtca aaaaaacaag gcgcaccacc accagtgctg attacaatgt
     6661 tttgttgggc atcaatgtta ttgataaaat ttctctccaa gtttctaaaa tatgcttcgc
     6721 ctttttgttc aaaaatttct ttaatactca tgccttcatt atcttcaata aatttatcta
     6781 agtcgatata gtcatattgc aaaagcggcg caagatgtcg cccaacatat gatttgccac
     6841 aacccataaa accaactaaa aaaataagca tttatgtaga atttattttg tttaaaatgt
     6901 gtgttaaaac aaagatacat aaatctatat tttcctatca tgaaatgata ttttattgaa
     6961 aaagttgaat gcgtttttat ttatttcatg ctctttttct tattatctat tgataaatat
     7021 tgccttatta aaaaatctta tttttgcttg tataacaaca tgaaataatc agcttattta
     7081 cttatttcaa tgagcactat tttagtaata attatttgaa caatggaagc aaaaaaagta
     7141 atcggccaaa catttacttt tcaaaatggc aacacggtga aaaaccgttt tttaaagtca
     7201 gcaatgagtg aaggtttagg cgacaaaaat ggagcgccaa cgacagcatt aaatacgctt
     7261 tatggcactt gggctgatgg tggaattggt atttgtgtta caggaaatgt gatgattgat
     7321 aaacgtgcac ttggagagcc aggcaatgtg attattgaag atgaaaaaca tttgccacaa
     7381 ttaaaggcat gggcaaaagc ggcaacaaga aataacacac attgttgggt gcagttaaat
     7441 catgtgggca aacaatcgcc aagaggctta aataaagaaa atgttgcacc gtcggcggtg
     7501 ccattccgca ctgatatgcg accttacttt gctactccac gcgagttgac tgataatgaa
     7561 attttagaca tcattcaacg ctttggcaat gcagcaagaa ttgtgaaaaa agccggattt
     7621 tctggtgtgc aaatacatgg agcacatggc tatttggtaa gccaattttt atcgccacat
     7681 cacaacatac gcaatgatcg atggggtggc aatcctgaaa atagaagacg ctttgtgtta
     7741 gaagtgtatc atgcaatgcg tgcgcaagtt ggtcctgatt ttcctgtaag tatcaaatta
     7801 aattctgccg atttccaacg tggtggcttt agcgaagacg aatctttaga tacaatacgt
     7861 gcacttgcag atgcaggtat cgatttatta gaaatatctg gtggcaacta tgaagagcca
     7921 gcaatgacag gcattgcgat aaaagaaagc acaaaaatgc gggaagctta ttttatggac
     7981 tttgccgaaa aagcgcgaaa agtttgtacc attccaattg ccgttactgg tggttttcgt
     8041 acagctgaag gcatggcaaa tgcgattagc tcaaatgcag tggattttgt cggcattgct
     8101 cgacatttgg caattgaaac agatgtacca aataaactct tgagcggtaa attgccaaaa
     8161 cacagcgtaa agccaataaa aacgggcatt ccgatgatag ataacatggg aattatggaa
     8221 gtgtcgtggt attcagcgca attaaaacgt attggaaatg gcaaaaaacc aaacgtagat
     8281 gcaagtccat tcttagagtt tttaaaatcg attttccatc aaattggcat taccaaaaga
     8341 ctgcctagca gattaagagc taagtagtgt tgtacggcat acgtctttta ttagcgtatg
     8401 cattgaatta aaaagctata tcatgtgatg tattattcta tagtgcatca aaaaaactat
     8461 ttaccaattc taattcttgg atctaaaata gcataaagca tatccactaa gaaattcatg
     8521 atgataaaaa cagtagcagc aaacaagatt gaacccatga ctactggaaa atcgaatgtg
     8581 ttcaagcttt gtacagtttt acttcctaaa ccatctaggt taaacacaat ttctacgaaa
     8641 taagcacccg ctaataaaga ggcaaaccag cctgaaatac ttgtaattac agggtttagc
     8701 gcatttcgta atgtatgttt aataatgatt gtcgcttctg ataagccttt tgcacgtgca
     8761 gtgcgcacat agtcataccc taatgcatcg agcatagaac tacgtgttag ttgatagata
     8821 atagcaacag gtcgtgtgcc caaagcgata gtaggtaaaa tgagattttt tagtaaaagc
     8881 acttcttcgc cgtaatcgtt aatccaaaat aatggaccac taacttcaag cccagtaaaa
     8941 tcgtgccact tatagccaaa gtataaacta agcaccattg ccgtaacgaa ggaaggtgca
     9001 gaaatccaaa gcacagaaat tgacattgca atgttatcga accacgaatg ttgcttaagt
     9061 gcgcctataa cacctaatgg aatcccaatg ataatagcca ataggataga agtaatagcc
     9121 aataaaacag ttgttggaat tgttcgtgct aatatttctg agacattttc tttcgtttgg
     9181 taggagcgtc ggagataagg catttttagc actaatgctt tttctttgct tactgcaaaa
     9241 agttttgtgt agttatattt ctctttagct tctttagtat tttcgtgaac agcaataggc
     9301 gagaggtcgt ttaaatagag tgctaactgc acatacttag gcttgtctaa tccaaattct
     9361 ttgtttatcg cttctacaga ttcgatatcg gcgcgctgcc caagagtaag acgtgccggg
     9421 tcgacaggta atatattgaa caagccaaat atcacaaata ctactcctaa aagtacaagc
     9481 aagccgtaga atagtttatt gaaaatgaat gttgacatca tacaatgaca agttatttat
     9541 tttagtacac tttttttcaa atcagcataa gtagttaatt cattaaaatg atatttagcc
     9601 acaataattc cttttttcaa aattaataaa cctggatttg agcgcataat tgtttttagt
     9661 ggcgttgcat cagtgttata aatttcgtaa tcgtattctt tgttgtgttt ttcttcaaat
     9721 gctttcatgt catcaatcaa actgccagtt aaaatataca cgagtttgcc atcgtctgct
     9781 gctgcatctg ttactttcaa taatttaata taaccatctt catttacctt ttttaattcc
     9841 ggaatgatga taaaaaataa atattcttct tcttcaaaaa atacagaagt ataatcttcg
     9901 ccattcgcat cttttatagc aaaatctttc aattttggat catcaccttt ttgaatcatg
     9961 acatcttttc tatctacgaa atcccaattg tcgatgtctt gtggccatgt gcctttaaat
    10021 tcttttactt ctccagtttt tttattttta taaataaatg tgttttcata cttataaggt
    10081 ttcgcatttg caggtaattt taaaccttct ggaatagaac tgccaacctg ataaggtcta
    10141 aaatctttta taggctcgaa gtaaatattt ctgtaagcaa atgcaatcga tgcaattgtt
    10201 aataatagca ataggccacg attgattatt ttaccaaaga tttcttttat tgatttttga
    10261 ccaaataaaa taattagaat aagtacacac aaaaatacat ctttataaaa cgattgtatg
    10321 ggtgctaatt tcaagaaatc accgaagcaa ccgcagtctg ttacttttcc agtttttgca
    10381 gaatagccag ttaagaatgt aaagaaagcg attaataaaa ttagcaacca aattgtcacc
    10441 tttttccaag tgcctaatat taatgcaatt cctaaataga tttcaaaaat aatcataaca
    10501 aacgaaacag ctaaagcatg ttctgccatg agtttccata gaaatgaaag taaaggtgcc
    10561 gatttttcaa aaatctcgaa atattcttcc atttttattg cagttccagc cggatctatt
    10621 gcttttacca caccagaaaa cacaaaccaa acgccaacaa aataacgcaa gaaattgata
    10681 ggcagattac tttgcttaaa taaaaatttt ccaattagtg taaatacaac agcaattgct
    10741 gcaaacattc caataagtgt aattaaatcc atgatattta ttgtctaata ataatttgag
    10801 ttaatttttt tactattctg ttttattttt cattcagctt tatcaatgca aaaatggcat
    10861 aatttactat gtccatgtag ttggcatcta ttccttctga aataatagtt tgtcctttat
    10921 tctcttcgat ttgtttgatg cgatgtaatt tagataaaat taaatcggta aacgagctga
    10981 tgcgcatatc gcgccaagcc tcgccatagt cgtggttttt ggctaaaaat agttttttta
    11041 taatgctgta ttgctcatcg aaaagcactt cagcttcttc gtatttgagt tcaatagcat
    11101 aatctttttt gtgttccaat tgcaattgaa taatcgcgat gatgccataa tttacgcaac
    11161 caataaattc agattcgatg ctgtctccaa ctttgttctc gccagtttca tcaattgttc
    11221 tgatgcgttt tgctttaata taaatttgat cggtaataga acgagcacgt aagatgcgcc
    11281 acgaggtgcc atagtctttc gtctttttca aaaaaacatc tttgcacgag gcaaatgcgt
    11341 tttcatattg cgtaatagtg ttttcgttca cagtaaaatg ttattcagtg gctaaaaata
    11401 cataactttg tgctgatatt gccgtgaatt cgctttttta tgaaaaaatt tacactgaat
    11461 tgtaaaggta aactctttgt tgcagcacaa ccattgataa tgggcatttt gaactgcaca
    11521 cctgattcat tttatgatgg tggcaaattt ccgtctatag atacattgct tcagcaaacc
    11581 gagaagatgt tacaagaagg tgctgatatt attgatattg gtgctgtgtc ttcaaagcca
    11641 aattcggttc aagtatcagc agaagtggaa attaatcgta ttattccaat tgtagaagct
    11701 gttcatcgtc attttccaga agcattaatc agcattgata catttaggag tgatgtggca
    11761 gagatttgca ttcaacatgg cgctactatc attaatgata tctcaggtgg tcatgccgat
    11821 gaaaatatca tgcgtgttgc tgcaaaatat cactgtcctt atataatgat gcacaggctt
    11881 ggcgactttg aaaccatgca tcaacagttt gaatatactg atgtaattac ttctgtgttt
    11941 gattattttt ctgcaaaaat cgaaacagca aaaaagtttg gcataaatga tgttatcata
    12001 gatgtaggtt ttggtttttc aaaaacaata ccacaaaatt atacattatt gaaaaattta
    12061 tccatttttg agcaattgaa taaaccaatt ttagtaggtt tgtctaggaa aagtatgctg
    12121 tataagttgc taaatacaaa tccagaaaat gcattaaatg ccacaacagc ggtaaacagc
    12181 attgctttgc aacaaggtgc aagtattttg cgcgtgcatg acgtaaaaga agcagctgaa
    12241 tgtgtgaaaa tttataatca actacaggct atatgaaaag tgttttgaca tatctgcttt
    12301 tgtttttatt tgtaaactca ttacaagcaa atgatgctat agaaggtaaa tggcgtggca
    12361 caatagtcgg acatgttgac gatagagatt attttattca tgctgaaata gcagcaaaaa
    12421 agcgcgaagg tgagtttact ataaaaatga aactctttag cgaagattat acaggcgaat
    12481 ttttattaca aacagaaatg atagcagaaa aactatacat cagaaagttg gaaaaaatag
    12541 ctgaatttcc atatccttat ctgcatatcg aagactgttt tacaggatat ttcttgttga
    12601 agaaatcggc aaataataaa atggaattag acttatatcg caatcctgtt tatcataaaa
    12661 tagaagaatt taccaatctc gatggtgatg gaaatttttc gccaggattt caatgtttta
    12721 ccactgttat attgcgctcc aaacatagtg atacaacatt taatgctaaa gaaaaacgct
    12781 cagactcttt ggtgactgcc aagaaaattc gcgcagatga agtgttgaaa cgcaaacaag
    12841 tttctaccaa aacttggact gttaagaaaa ataaaatagt attgcaggtt tgggacaata
    12901 acaaagaaga cggtgatatt atttctctaa agtttaatga taaatggatt ttgacagatt
    12961 ttttattgaa gaaagagaaa cataccattc ctttgacttt gaataaaaaa gaaaatcagt
    13021 tgttgctatt tgcagaaaat ttaggaagca taacgccaaa tacagccgct gtttccattg
    13081 atgataatgt attggtgcgt acttttattt taaattcaga catgaataaa agtgaaacaa
    13141 taaaaatatc tttagccgat actaaagtga aataatctat ttcaatgctt tcaacaattc
    13201 ttctatagaa aaagtatcaa tgccttcttt ttgtgctaat gctaaagaaa tttccgcata
    13261 tttttttgcg ctcgatttgt tatttaattt ttgatataag cgcgcaactg tatcattgtt
    13321 atcgaaatta ctatttaatt ctacagatcg caatgcaaat tgtaatgctt tgccaagcgt
    13381 cgttttatct ttatagtttt cccataatgt gccagcaatg ctatttaaac gctcgaaatc
    13441 atttttacag aatttatcgg caaatgctaa tgcttttggt aaataggctt tccaattggc
    13501 agctcgcttt agtaagctta attcaaactc ggcacaaaga atttccgttt tttctggtaa
    13561 atattgttga attatttttt tgaaagcttc tttgtcagtg ccttttttac tggctttttt
    13621 tgcttcaatc cacactaatt cttcaatctg gttgtccacg tttttaatgc caagcgtttc
    13681 ataaaaaagt gttttattat cttgtaaaat ggcatatcca gtgtcgtgaa tgctattcgt
    13741 caataggctt attaatctga tattgtcagg cgataaatta tagtttgatt gctgcaaata
    13801 atcttttaat gctggctgtg ctgattcatc ttgcgcattg tttgcattct ccacaaaatt
    13861 tcttaaaaat gctgtatcac gcacaccact ttcatatttt tttttaagtg ttataaattg
    13921 catttctggg tctaatgtat tgttgcaaag agtaataaaa tcagcagcag gcatagaacc
    13981 taagcttctg tgcactaact cgccgtttgc atcaaaaaat aaataagtag gatagctacg
    14041 cacattatat ttttttgcta attcgatgcc ttcgcctttt tccatatcta tttttgcatt
    14101 cacataattt ggatttaatg cttcgccaac ttcaggtttt ggaaatactt cttttgccat
    14161 ccatttgcaa gggccacacc agctggcata tgcatctaaa aagatgaatt tattttcttt
    14221 ttttgctttg tctaaaattt gttgccagct agcaccattt tcaaattgca tgcctgtgtc
    14281 ttcagcaaaa gtgagcgtac tcatgcacac aaaaatcaag ataaatatat ttttcattct
    14341 cttttttatt attgtattgc aatatgtttt ctgttatatc aaatctaaat caatctttat
    14401 tggctttttg ttttcgccta cagctacaaa tttaaacatg ccatttattg ccaattcacg
    14461 tttgtccgaa ttcatcagtt ctataaaaat ttctacttta atatctaagc tcgaatttcc
    14521 cattttaacg actctaccta ctaactcaat aatagtgcca cttggaattg gaacattaaa
    14581 atcaatttta tcactgctta cagtcaccat tttcaaacgt gcaaatcgtg tagccgtaat
    14641 aaatgctact tcgtccatta gctgcatggc agtgccacca aataaggtgt cgtaatgatt
    14701 cgttgtattt ggaaatactg ctttgaaaat atgtgtttca gataatgctg ctctttgttc
    14761 gaatgtcatg ccacaaagtt aatacaagta tctaataaaa tactatgctt gttttttgtt
    14821 gtttgtcaat atttatttct gcttttagat tgcctttttt tgataaataa tacatgcgaa
    14881 caatttaaat gtgaaataga agcaattact tacttgacaa tttcataaaa tatattaata
    14941 tttggctgtt aattgatagc aaaaacctaa ttgccaatga aagttattca ttttgaaatt
    15001 ccggcaaaaa atccggagcg cttaaaacat ttttattcta agatatttga ttggcgattt
    15061 tgtcaatgga aagaagctgg tttttggtat gcacttacag gcagtccttc tgaatttgga
    15121 attgatggcg gaattataga tgttaaagaa ttagacattc ctgtgaatag catgattgca
    15181 gtagaaaaca ttgacaccac attgcataaa attgtagaag ttggtggcaa agtggctgtg
    15241 cctaagtttt atgagcaagg cgttggttat ttagcttatt ttaatgattt agatggcaat
    15301 atttttggct tgcgcgaagt aaatcctaat gtgtaaatta tcggcatgaa tagagttgta
    15361 aattttgatt tcccaacaga taatcctgaa aatgcgaaac atttcttttc cgaaattttc
    15421 ggctggaaat tcacagaatg gctcaatgaa gaatattggc tgatagaaac aggagacaag
    15481 cataaacaag gcattaatgg cagtatgatt aggcgaagac atgagcaaca aatgtcaagt
    15541 attatggtgc aagtagaaaa catcgatctc gttttgtcga gaatacaagc agaaggcggc
    15601 gaagttgtat ttcctaaaca agtcgttccg aaggtcggat atattgccta cttcaaagat
    15661 cctgaaaata atgtgatatg cattatggaa tttgatgtgt ccgccaaata actacatttt
    15721 tctatttttg atctgtgtta aaaatgcatg taggaatacg gcaaacaaaa taattagggt
    15781 tccacaataa aactgccagt ttaattcttt gttttctcct aaaaatataa tagctaaaat
    15841 tataccataa acaggttcta aattattaat gagtgatgta gtgaatgcat cagtaatttt
    15901 taatgttttt aaaaataaat tatatggaat taccgtacaa aacaaagcca atactaataa
    15961 taaaaataca tctgtttggt gcggaattgg aaattcattt ttactaaaat ataaatagag
    16021 tggcattaat aaagtaagga atataaaacc gctcagcatt tgtataaatg taatcacatt
    16081 tgcttgtaca tcttgcgtat attttttatt taagatacca aaaatagcag caataaatgc
    16141 agctacgata cccagaaaga atccaaaacg ataatgaaaa tctaatgatt gattgataag
    16201 caagatgcca ggaataacaa aaatactcaa taataaattt gaagttttaa atggtgtttt
    16261 tgtaaatatt ggttcgagaa tggccgtaaa taaactcaca cacgcaatgc aacttacagc
    16321 cactgatgca ttggcatatt tgatagaagc ataccaagct acccaatgta tagacaataa
    16381 tatgccgatg ccacttattt gcaagactgt ttttttgtcg agttgtcgaa cgtgttttat
    16441 gatttttgga ataaataaca ttgcgattga tacaaataaa acacgatacc atactaagct
    16501 aaattcgccg atgctgatta attttcctaa aatagcggtg agaccccaca agaatgtggc
    16561 gatatgcatt tgtgtatatg ctttgctgcg caattgcatg ttgcaaaagt aggaaacgct
    16621 atgataagta taaaatttaa ttctgatttt ctaaaatatg cttatcttta aaaatacact
    16681 tattcaacta aaggcatatg gacaagaaat atcaaacttt ttggagcttt tatccttatt
    16741 atttgacaga gcatagcaat ccaatttgca gaataacaca ttatattggc acatcactat
    16801 tgtttgttgt aattgccttg tcaatttata ctagtaatgc caaattattg tttttactgc
    16861 cacttgttgg ctatggcttt gcatggttag gtcattttgt tttcgaaaaa aataaacctg
    16921 caactttcca atatcctttg tatagcttag gttcagattt tgtgatgttt tatcattttt
    16981 tgacaggtca agtcaatcaa aaagtagaag atgctaagaa aacagtagcc tcacaatcat
    17041 aattttccat cttattttaa tacgtttttg tctaaatatt aaaatcttaa caatttaagg
    17101 attgttaacg ttttgcaaag ttcactcgtt gtaattttaa ggtgtgaata aaaatcttat
    17161 cctctttact tctattttat tattcctcgg cttatttggt attggaatag tgcaaatttt
    17221 ttggttgaaa ggcgccataa aatcaaggga acaagatttt gacaaagcag tatttgaggc
    17281 catgaatgat atgtctatca aaattgagga tttatcatac aagccgatat tatccaaaat
    17341 gttgcagtca caaaagatac atacaaatga atatggcgaa gtcatcatag aaatgtctga
    17401 tgataatgca gactacgaat cggcgtatgc tgagccacca cacggcgagt ttccatcgaa
    17461 cggaaaacat aaaaaaaata aagcgattca gccaaatgct ttactgccaa tgtatgaaga
    17521 tgcgaatggc aatgctgaca attcattccc attagaaata gataatctac aacagtttat
    17581 ggcacaacaa atgacgagtt tgcagcctat tactgagatt ttagatacca ccaaattaaa
    17641 agaaattatt acagcagaat taaattcgca tggcattaaa acaaaagtaa aatatggcgt
    17701 gacagaatat gcgcctaata atttcgtgct tttgtctaaa caagctcctt tagcagaatt
    17761 gtttcgtacg ccctattctg tcgatttgtt tcgtcgtagt atgtttgatg ataataagaa
    17821 attgaaatta atttttccag ataaaaagaa atatttatac tcgacgatgt cgccaatgat
    17881 attatcatct tctgtttttt ttctaatggt ggttgccgca ttttttctgt cgtttcaaat
    17941 aattttccga caaaagaaat tgtctgacat gaaaacagat ttcataaaca atatgacaca
    18001 tgagctgaaa acgccgattg caactatttc tattgcgagc gaaatgctaa aagataattc
    18061 catttctgca tcacaagaaa atagagcgaa atatgcaggc attatcttcg acgaaaataa
    18121 aagactttac aatcacgttg aacaagtttt gcagattgca cgtttagaaa aaggtgaact
    18181 gcaattgaat tttgaagacc gcgatgtgca tgcaatcatt acggcaactg ctaatcgttt
    18241 taatctacga ttagaagaat tacaaggtag tatagctttg caattaaatg cagaacaatc
    18301 tatcttcaaa atagatgaaa tgcatttcac aaacgttata aataatctca tcgacaatgc
    18361 tataaaatat aatgataaag cacctttaat aaaaattaac actttcaaca ttatcacagg
    18421 catacaaatt gaagtaatag ataatggcgt tggcttggca aaagaagacc aacagaaagt
    18481 ttttgaaaaa ttttatagag taacaaaagg taatgtgcat gatacaaaag gctttggctt
    18541 aggtttaagt tatgtgcaat ctatcataga caaacatagc ggcaaagtat gggtcgaaag
    18601 caaaataaaa gaaggctcta catttattat tcaattacct tcaaatgtat aaccaataaa
    18661 aaataaaaat tatgacgaca actgcaatta aaaagaaagt actattagta gaagacgatc
    18721 caaatttagg tatgttatta caagaatatc ttagacataa aaattttgaa gtagaactaa
    18781 aacgcgatgg cgtggaaggc ttatttgctt ttagaaaaaa tggctatgac attattttat
    18841 tagatgtgat gatgcctaaa aaagacggct ttacagtagc agaagaaatt agaaaagaag
    18901 acaaagatgt gccgattatt ttcttgactg caaaatcatt gaaagaagat aaaattaaag
    18961 gattaatact tggcgccgac gattatatta cgaagccatt tagcatggaa atattagagt
    19021 tgaaaatgaa tgccatcttg cgcagaactg aaaaacatga agtacaagta gcattagatg
    19081 agtatagagc tggcgattgt gtgctcgatt ataaaaatca taagttgcac gttaaaggtc
    19141 aagagataaa attaacgact aaagaaaatg aattattgcg tttgtttttt gagaagaaaa
    19201 atcaaatctt agaacgcgaa ctagcattaa agcacgtttg gcaagacgat agctatttta
    19261 cagcgcgcag tatggatgtg tttatctcaa aaatcagaaa atatataaaa gatgatgagc
    19321 gtttgcaaat attaaacata catggacaag gttataagtt ggtagaaaat gaggctgtgg
    19381 cttaatgaaa aatgacgagc atagcacaat ttgcttactg taaaataata cgatgattta
    19441 tgatttcatt tgtaaatttg caaaatgata ttgacagata aaggcatttt agccgaaatg
    19501 gaaaaaggga caatcattgt tgagcctttc gatagagaat atttgggaac caattcttat
    19561 gatgtacatt taagcaaaca tttggcaact tacacgcagc cagttttaga tgctaaaatg
    19621 cacaatacta ttcatcattt cgacattcca gaggaaggtt ttttgctcga accgggaatt
    19681 ttgtatttag gcgcaacgct tgaatatacc gaaacacatg cgcatgtgcc ttttttagaa
    19741 ggtaaatcca gtatgggcag acttggcata gacatacatg ctacggctgg aaaaggagat
    19801 gtcggctttt gtaaccattg gactttagaa atatcttgca ttcagccagt gcgtatctat
    19861 gcgggcatgc cagttggcca gcttatctat tttgttgtcg atggtgaaat agaaaatgca
    19921 tataacaaaa aagtatcggc gaaatacaat caacgtactg ccaagccaat ggagagcatg
    19981 atgtggaaga attttaaata agttttacct ttttgtttct tgaataattt ctaatgatta
    20041 tgtgataatt ttgtaaaaaa ttacacgatg agccctttga ataaagagca attattagtt
    20101 gacttaaaaa attggaatca aataattagg aaatatcaag tgcccagcac gaaagaagcc
    20161 ttaattcaaa tagccaatag ttttagcttt tatttgatat tgttggcatt acaattctat
    20221 ttatacgata agtcattctt ctggtcggca ttagtcgcgc tactgaatgg ttttatgctt
    20281 tctcgtattt tcatcattca acacgattgt ggacacagct cgtttactaa atataaaacc
    20341 ttcaacgaaa ttattggtac cgtttgtagt gtgtgtactt taattccata tcgctattgg
    20401 gcacgcaatc acagtttcca ccatgcacac aatggtcaat tagaagtgag cgatatcggt
    20461 gatattgaag tattcaccac agagcaatat gctaaagcaa attggcgtca aaaattatat
    20521 tatcgcattt acagaaatcc attttattta tttaccattg gcggatttat ttatgttgtt
    20581 atttacaatc gttttgcatt tgtaaatagc gattatttta aaaaagtaaa acaaagcgtg
    20641 aatatcagta atattttatt tatagtcgta tatgctttgt gtgcctattt atttggtgtc
    20701 gaaagatttt tagtggtgca gtttactaat ctgtttttct ttggaatata tgcactttgg
    20761 tttttttata ttcaacacca atatgaacat atttttaaag caaagaaaga agaatggaat
    20821 tatgtagtgg cagctatcaa gggtagtaca tactacaaat taccacgtgt ttttcattgg
    20881 ttgtctggca atattggcta tcatcatata catcatttaa gtccagctat tccaaattat
    20941 aatttgagaa aatgtcataa cgaaaaccct gtttttttga aatataccaa cacacttacc
    21001 ttttggcaaa gtttaaaaac tttgcgctct aacttatggg acgagcaaca aaaaaagatg
    21061 gtttcttttg gagaatataa aagaaacaga agattaaaaa gcatgatgaa ataagatttt
    21121 ttcttttctc acttgaatcc agctttagaa aattagtacc acaacattat tagttaccta
    21181 atgattttat tcaagtatta ttgcaataca ggagaattta tgatacaaga tcataaatac
    21241 gcaatgaatc tgattatagc taatttctta aagccataat aaaaccaaag actagtagcg
    21301 gtgtaaacac aaaccattct aaaccggcag ctgctaatat tgaaccacca ataatacaac
    21361 aaccaccaaa aatagtgttc tgcaaaccaa agctgatagg tttctttttc tctactaatt
    21421 ttgtttcgta ttgtttaaat gcttcagaga taactaatgg cgaacgcatg ataatgtcca
    21481 tgatttcagg tgcattttgc agttgtgatt ttaataaatc gactggattt acttcgccta
    21541 aaatcatttt gcggatatgt tttttggtta ccttttgaat gtcgaatcct ggtaaaatga
    21601 catttccaac gccttcatac gtaatgatgg cttttaccat caatactaat tcaagcggat
    21661 aatacattcg atattttgca cctaaggcaa cggattctaa gataagcttt gccaaggaaa
    21721 aatctttaaa attcggtgct ttcaaccatt tgtttcctat tgatacaaac tcttttctaa
    21781 agccttctac atcgctgcct ttgcctttgc gtgctaataa agttagatat ctggcagcac
    21841 cttgcggatc gcctgtcacc aaactattaa agtagtatag catggtcttt cgtgtggtat
    21901 cttcaaatct gcctaccatt cctaagtcga taaagccaac ttgtggacca tttggaccgt
    21961 ccataatcat taaattgcct ggatgtaaat cagcgtggaa aaaaccatca tgatatagca
    22021 tttgaataat ggcataagcg ccataatcta caattttttc tctatctgcc tcgctatatc
    22081 gctcgtacgc acgatggtca ggtttgatgc cacgaatgaa ttgcatacaa attaaatcac
    22141 tggtagaata ttctctgaaa attcgtggaa aatgaatttc tggatgttta gcaaaattag
    22201 cgctgaactc ttctgcatta tctgcttcta acctaaaatc tacttctcga ttggtataat
    22261 cacaaaattc ggtaaacata tttttcggtt gcaaatgcgg aataaatatc tccaaaaatg
    22321 cccctattac ttttatgatt ttactatcgg tttgtataag ttctcgcgtg cctggtttta
    22381 ttagttttaa cacgacttct tcgccagtgc gtagtgttgc acgatgtgtt tgtgcaatag
    22441 aagcagagcc taaaggattt tgttttactt ctgtaaatac tctatgtaat ggttgcccaa
    22501 tttgttcttc tactaattct gcaaatcgat cataaccaat ggctggcaag gtgtctaata
    22561 gttttttaag ttcatcagta atgtcttttg gcaacatatc ttcgcgcaag ctcataattt
    22621 ggccaagctt tatatatgta gcacctagca tttccaatct tctgcgcagc tgaactgcaa
    22681 aaggttgttt gatgagattt ttctttaata taggccaatg caaccaaaga aatagacgta
    22741 aaaagatatc aattccaata ctaaattttg cttttgtctc acgtctatga cgcaagtaag
    22801 cataaccgcc acctaacaat aagccagaaa gatgtctatt cgtgataaga aatcgaacga
    22861 acaaagaact tggtttcttc tcgatgtatt gttcaaattg gcgattggca tcttgatctt
    22921 gtggatgaag gtcgatgttt tcttcagttt ctgttatttc catagattac aaaaaaaata
    22981 tttttgtaag atactacata gtaggtatat atccaataat ttaaaaaaga attcagaaaa
    23041 aaagctgctt tttttgagat ttttttgatg gcataagttg taaggcaagt ttatttattg
    23101 ccactttgtt ttgcccgaat tctactgact gtttctgcgg tcatgttgag atagctcgct
    23161 acatttttca tagaaattct gttgataata tcgccatact ctaattcaaa gtcgcgatat
    23221 cgttcttcgg aatttgctgc ttttagtgta tataatttat ctacataagc aaaatgaaat
    23281 acttccagct ggcgtctgta aaggctttca attgatggat gttttttata taaattttct
    23341 aaatcttcgt gttttatttt gagcacaact aaatcttcta atgattcata attgtgtacg
    23401 cctggatact tggataaatg atcgtaatta ccgccaaaaa agccaccttc ttttacaaaa
    23461 cgttcaataa ttaatttatc atttttataa aaataaattc tgatgatgcc tttaaaaata
    23521 aaatagatat gtaaaatagg ctcagtttca cgaatgatat gtgtgttttt tttataagtt
    23581 tctaaggtga aatagctaaa taattcctct aatgcatgct ctggaatagc acaaatttca
    23641 agaaattttg ctttcaatat tttttttgca acggactcta atttttgatc aacaagcatt
    23701 tctatcaaaa atgtttagct attttactat tttttttaat tatggcttct ctattctaat
    23761 accaacaatt tctacttttc tttgtttaga cgcagctaca ccaaattctg attttgctct
    23821 gtctttacca ttatcgctga tttcttttgg cgatagtgtt tcgccaaatg gaatagcaag
    23881 tgctgttttt gacaagattt taaattgctc gcccaattta gaatcgaagt aagcgatgat
    23941 gctgttaata cgtcgttcag atagtttttt attatagctt tcagatgcca atggcgatgt
    24001 ataacctcta aattcgagcg ttatcacata gccactgtcc accaaaccac ttaatgcctg
    24061 tactacggaa cttacgcttt ggaaattctt gtctatttga ctaaagaatg tgccaacatt
    24121 ttctgcacca tcaactgatt tgctttcgaa tgttgctttt ctggctgtat atgaattata
    24181 caattcgcca taatcaactt tgctattttg ttttggcata tcattatcaa aatataattc
    24241 aattggcaac atcgacatga ttttctctgt tgttttaacc acagtaacac ttggtgctgg
    24301 tgcttttatt gcgattggtt ttaaaattaa ttcaatagaa tcaatatatt gacgtggaac
    24361 agaatcagct ggaacgatgc gattaataat ggtatccact aaattattgt cggcatcttc
    24421 atatttcacg cgtacaatcg tttctacatt tgctggcaca tacgacggac atttaggaca
    24481 ttgttttgtt tttacttcgc caaattcgta ggtgagcgcc aattcaaaag caccttgtgc
    24541 tttcgatgct tttcttaatc ctgaaacatt aaaatcgtat gctgcgccaa cgcgaagcgt
    24601 tttatataaa aatgataaat aagcgatagc ggcatcattg ccgattgctc tatagcctgc
    24661 gccaaagcct aagccaaagt ttttcttttc attaaaacga acttctgcaa tcgcattaat
    24721 caacacttga ttggctttgc gttggttttg aaaaaatacg ccaggctgga tattccaatt
    24781 gtctttgatg ttgactttag ctttcgcata agcagcatgt cttgctggca gtttagatga
    24841 agtataatat aagaaatttt gatttggctg atgcaagttt gacgttgcag cgccaatatc
    24901 taacacggct ttttctttta ttttagttct gaaatttaag cctaatgtaa aattgggata
    24961 tgaaacatta ttgtttgaaa aatcttcgcc acttggcaaa ttaggatcga aagtagaacc
    25021 attatattga ttgtcgaatt gcaagcctaa ttcatctaat gatttgatgg taataccagc
    25081 agtaatacca agtgtaagca attgtttttc tttgttgaaa tattttccaa aattcatgct
    25141 gatatttgga ttgaatatgg ataaattgcc atcaccagct ttgtcatata agaagctgac
    25201 accaccacca atcaaccaac cttttggaaa tttttttaat aatctgtcat aagaggcagt
    25261 tgtcgtagaa tacggaaccg gcaatgtgcg ccattggtct ctatagtttc cagctaagcg
    25321 attggctttc ccatattcgg tgacaccagc taatgctgga tttaaatata atggattgta
    25381 ttgtatctgg ctaaaatgtg ggtcttgcgc cacaataagt ttcgttgtaa aaatggtcaa
    25441 taatccgaaa aatattattc ttttcatatc tgtaaatata gcctatttta tttaattttt
    25501 ttgccgttca caaagttaca cttaataact aaatcaaatg taatagttat cgtaaaatcg
    25561 tgatattacc ttttaatata atttcatcgc cttgtagaca ttcgcctttt aaataatagc
    25621 cgtaagcatc ttccattgac ggctgtccat tataggtgcc gtcccaacct ttgctgatgt
    25681 cgtctgtttc aaatactttc aggccccaac gattatagat aaacaagtgc atgtttttca
    25741 atgctgatga acgaacaaat aatatatcgt ttacgccatc gccatttggt gaaaatgcat
    25801 ttggtatata aatattttct tttacacatg gcaggtattc caatgcaata aatacagaat
    25861 ctaaagcgat gcattgtgta attttatttt gcgcactgac aataaaccaa gtggacgatt
    25921 gaatatgtgc tattggattc tgcaaagtgc ttacttctaa taattgagca ggagaccagt
    25981 tgtaagttaa attttcattt gtaattacat tcaattgtat aggttcagta gataatgctg
    26041 tggttttatc agcagttgca tctataatcg gcaattgatg tacaattaat gtgaggtgta
    26101 ctatgctatc gcatgtgttt gctgctgtaa atgtaatatc gtattcacca ttttcactca
    26161 atgtttgatt gccaatagta aattgttcac cagcgcaaat ttctccacgc aaaggtgtaa
    26221 ttggattttc aataacttct agctgtagat ttactaaact atcacaacca gaaacagcta
    26281 aaagtggaat tgaaaaattg cctgaagttt taaaaatttg tgtgccaata gctatgctat
    26341 cgcctttaca aatagtgcga tttatggttt cttttagcgt atctaaaaca tgaagtgata
    26401 agttaacaac actatcgcaa cctgcatttg cagtaagata aacagtgtag tttcctgttt
    26461 cattaaatgt ttgtgtgctg atggaataat gttgacctaa gcatatattt ttttcaatat
    26521 tttctatgat tgtatctgtt tctgtaaggt ctaaaaatac agtactatca caccctttag
    26581 cgcttattaa aggtattgta tagtttc
//
LOCUS       DAOQQV010000026        24915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_598_length_24915_cov_7.002695, whole genome shotgun sequence.
ACCESSION   DAOQQV010000026 DAOQQV010000000
VERSION     DAOQQV010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24915
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_598_length_24915_cov_7.002695"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLL59_04510"
     CDS             complement(<1..712)
                     /locus_tag="PLL59_04510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY23100.1"
                     /translation="MSEQQKQWFEVWFDSPLYHLLYKNRNNEEAAFFIDNVINHLQID
                     YGNILDLACGKGRHAHYLAEKGFNVVGVDLAKESINYANTLYQLPNLEFYVHDMRLPF
                     RINYFDYIFNFFTSIGYFSDIKENEKVFQSMHLGLKDGGYILIDFMNAEKVINNLVPR
                     ENIIIDEHHFYIRREVVNGKIIKHIQIEKGDNMWMFKEEVQALKQHHFHTFLNNTGFT
                     LVKEFGDYNLQAFHPKLSN"
     gene            complement(793..2145)
                     /locus_tag="PLL59_04515"
     CDS             complement(793..2145)
                     /locus_tag="PLL59_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMY23101.1"
                     /translation="MQLKGTYQEIWQISYPIILGSLAHSINQLIDTAFLGHSSKVALE
                     SITLAGIYYFVFVFIAGGLARGCQVILARYSGEKRDDKIGVAFDHLILIALILSVFLM
                     LFFKYATPYVLAFVVKSVAIQESAMAYINMMNYAVIPVVLGFCFNGFYTGIGKTKVIT
                     LATVFMAITNIICGYIFIFGNFGFSPMGIKGAGIATAIADTVLFLVYLCYFLYRKHHK
                     KFNAFTFKQIYFKQFYTIIRLSTPIILQNLIGIGSWWYFFLSIEKIGEHELAISGILK
                     SLFVFIGIPVWSLASTSNTLISNIIGQGRIDDVVPALKKVLTFSVGISLSINLIIVLF
                     PHQILSIFTNDTQLIQDTIAPFYTLMIALLFFSSGMIMNLGITGTGATFIPTIVEIFC
                     CLFYVLYCYYFVQIKQSPLYICWGCEVVYWLTLLVFTTTYWSTGIWRKYIKNIDSISK
                     "
     gene            complement(2218..3006)
                     /locus_tag="PLL59_04520"
     CDS             complement(2218..3006)
                     /locus_tag="PLL59_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY23102.1"
                     /translation="MFQNDLFKGKIVLVTGGGSGIGYAIAKQYLALGATVYIASRNAE
                     KIAKAITELKEIGDVRSAIADIRETAQIENLANQIKEEVGRLDILVNNAGGQFPSAAE
                     NISYNGFRAVVTNNLIGTFYVTQIMAKTFFIPQKDGNIVNIIANIFRGFPGMVHTGAA
                     RAGVDNMTKTLAIEWVRYNIRVNAIAPGIIQSSGMDTYPPAIIKGIEKSIPNQRMGTV
                     DEVAYPVLFLSSPMASYISGETLYVDAGNRLWGDQWKMWSESLT"
     gene            complement(3125..4030)
                     /locus_tag="PLL59_04525"
     CDS             complement(3125..4030)
                     /locus_tag="PLL59_04525"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMY23103.1"
                     /translation="MKSPFYKGIFFVFLGAICFSTKGIFAKLAFRYNVDGLQILMLRM
                     LFALPFYVAILLHEMRKEKKAIKRRDWSDVILLGLIGYYLAALFDFLGLQYVSASLER
                     NIIFTYPTFVLLMSKIFFKRKISNIQITAVIICYIGILLAFYSDKAAYVSSNLTKGTI
                     YILLSALTYALYLVKSDKLINNIGTIRFTCISMIVSCLAVLFHFTFIHGLDIFNFAKE
                     VYFIGLAIAIISTVLPSFLMSKGIQLIGSSNMAIVASIGPIATIFLSYYILQEQFTFL
                     HLIGTLLVLAGVLMISIKGNNKVVE"
     gene            complement(4088..5053)
                     /locus_tag="PLL59_04530"
     CDS             complement(4088..5053)
                     /locus_tag="PLL59_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23104.1"
                     /translation="MSELENGGSNSNSKVHAMYILLIALLIGGLVYTNLKLKKSKETI
                     VVTEKQRDDVTTLKQELDKKYAESLQEIESYRAENAGLDSLLTVKEQELSAKKAKIDA
                     LLGQISTLKSGDATKSKLLADAQALIAQMESDKAHLQASIDSLLTVNKALYQERDSLG
                     NELTTTTQRKQELDEENQKMKDRIDKASILSTANIQSTPIRITKKGKEDEVSKAKDAE
                     KLRVCFDLLQNKIAPSGQTAIEVRIISPDGTTIQMQNLGSGTFKEATTGNEIPFTYSI
                     SPDYQNESKTVCSYWAQTFKFAPGKYSVEIYEKGFLIGQSSFLMK"
     gene            5170..5898
                     /locus_tag="PLL59_04535"
     CDS             5170..5898
                     /locus_tag="PLL59_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HMY23105.1"
                     /translation="MLENIKLTDVLFIDIETVPNVSDFNNLTPYEQELWEEKKGKQRS
                     EDESVANYYFNNAAIFAEFGKIICISVGRITAEGMFQEFTLRSFYGDNEVKILTDFTS
                     FLQEQFKSNRNITLCGHNIREFDVPYICRRLIMNNLKLPAYFESLQMKKPWEAGLLDS
                     MDFWKFGDYKNYISLKLLAFCLGIPSPKDDISGKDVGKVYWQEQGLERIKTYCQKDVL
                     TVAQIVLKLKGISLISESNIRIVN"
     gene            5961..7742
                     /locus_tag="PLL59_04540"
     CDS             5961..7742
                     /locus_tag="PLL59_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMY23106.1"
                     /translation="MAELLLDVKNLVTEFNTENGIVKAVDDVSFQVNKGEVIGIVGES
                     GSGKSVTSLSIMRLIPNPPGKITGGQINYYRDGKTAIDLVKIPDNEMRAYRGNEISMI
                     FQEPMTSLNPVFTCGNQVMEAIMLHQKISKDEAEKKTLELFAQVKLPDPKRALLAYPH
                     QLSGGQKQRVMIAMAMSCQPKILIADEPTTALDVTVQKTILELINDLSKQTGMSTIFI
                     THDLGVIAEIADRVVVMYKGKIVEQGSVVDIFSNPKHPYTKGLLACRPPLEKRLSKLP
                     VIADFMEEKDGAILEKAADMHALVDAFVVSQQQTEKRFNDLQQHAPILQLKNIQTWFP
                     NKTNMFGKPLDYVKAVDDVSFDVHEGETLGLVGESGCGKTTLGRTILRLIEPTGGKAI
                     FDGKDIFNLGQKDLKELREHMQIIFQDPYSSLNPRMTIGNAILEPMQVHNKYSNDAIR
                     KDKVIELLEKVNMKPEHFQRYPHEFSGGQRQRICIARALALNPKFIICDESVSALDVS
                     VQAQVLNLLIQLREEFKFTYIFISHDLSVVKFMSDRMVVMNKGKIEEMGIADEIYNNP
                     QQEYTKKLIAAIPKGNLADIKARFERV"
     gene            7823..8452
                     /locus_tag="PLL59_04545"
     CDS             7823..8452
                     /locus_tag="PLL59_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HMY23107.1"
                     /translation="MKSSLTASEIRAAFLDFFKSKQHEIVASAPLVVKNDPTLLFINS
                     GMAPFKDFFLGIGKPSHPRIADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFG
                     DYFKKEAIAWSWELLTEVLKLEKDRLYVTIFEGNEAENIPKDIEAYNEWKKVIAEDRI
                     LLGNKKDNFWEMGDTGPCGPCTEIHVDCRSDVERKLLMALPWSMQIIHK"
     gene            8383..10455
                     /gene="alaS"
                     /locus_tag="PLL59_04550"
     CDS             8383..10455
                     /gene="alaS"
                     /locus_tag="PLL59_04550"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMY23108.1"
                     /translation="MPFRCRTKTIDGATLVNADHPQVIEIWNNVFIQFNRKKDGSLEQ
                     LPAKHVDTGMGFERLVRVLQGKSSNYDTDVFSGTIQSTENIVGKKYDRSDSKEAIAFR
                     VIADHIRAVAFTIADGQLPSNTGAGYVIRRILRRAVRYYFSYLDKKEPLLHLLVPVLA
                     TQFENVFPELKKQELFVAKVILEEENAFLKTLDNGLKRLDNIIKESDKIIEGKAAFEL
                     FDTYGFPIDLTNLIAAEKGLSVDENEFESELQKQKERSRAATELNVDDWITLSDGQSI
                     FVGYASLETKANVLKYRKIKAKGKEQYQLVLDKTPFYAESGGQVGDVGVLQFKDSKIL
                     ITDTKKENDLIVHFTDEIPADMSGEIIANVNVEIRKKTTIHHSATHLLQAALQQVLGK
                     HIAQKGSLNNAEYLRFDFSHFSKMTNEELENVEQLVNAKIRENIPVVIKEMPKEEATK
                     LGAMALFGEKYGDVVRVVIIDEKYSVELCGGTHVGATGELGLFKITSESAVAAGVRRI
                     EAVCGEAALAYVNTKISDLNAIKEALKNPKDAVKSIEVLQDENASLKKEVEKYQNEKV
                     EIIKQQLRSKVTNRNGANVLFEKVVGVNADSLKKIQFDLKNEFENLFFVASTIVNEKP
                     LLSIIISDDLVKSNNLHAGNMVKELAKAIKGGGGGQAFYATAGGNDASGIDTVLKQAE
                     ERFLILTS"
     gene            complement(10554..11270)
                     /locus_tag="PLL59_04555"
     CDS             complement(10554..11270)
                     /locus_tag="PLL59_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23109.1"
                     /translation="MSCEKEQQRLTDAPSSPEDALVKTKTEKGITETYYYDSQKRISK
                     ILFNTGSTSYFHEFTYSSNEIQEYKSEEPRYALEETLPSGATKYNRNSNPIVYKLNKQ
                     GYYVGLNSFYQTQNMEYDDAGFMRKQSLGIQDYSINEDYTNDGKNVEKMIGKGFSYVG
                     GNFTINQSFIYYSDKINSIGNRNFGKSFLGKSSTNLLMTSIEDGETAVFTYEFDANQR
                     VIKKTETKGLITNVTTYTYY"
     gene            11541..12977
                     /gene="gatB"
                     /locus_tag="PLL59_04560"
     CDS             11541..12977
                     /gene="gatB"
                     /locus_tag="PLL59_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HMY23110.1"
                     /translation="MEDKYEIVIGLEVHAQLNTKTKIFAADETTFGAAPNTQISPITV
                     GLPGTLPRLNKEVVELAVKLGIALDCHINEVNFFDRKNYFYADLPKGYQISQDRQPIC
                     VGGFVEIVVNNEPKKIALSRIHMEEDAGKNNHELDPDFSLIDLNRAGVPLLEIVSEPD
                     IRSSDEAYAYVTELRKLVRYLDVCDGNMEEGSMRCDANVSVRLKGETNYRNRVEVKNM
                     NSIRNVKRAIDAEVKRQIEIYENGGTVDQETRSFDPVDASSFTLRSKENAHDYRYFPE
                     PDLPPVVISSSTIEQIKSQMPQLPKALQTLFTQTYNLTAYDAQVLTDDKETADYFMAV
                     IQYTSNYKAAANWINNDVKSLLNEINCSLKDFAVQAKQLAEIIQLLDDNIVSSSGAKT
                     IFKAFVDGNKSSAIAVAEALNLIQSSDKDELTKWIEAALAKYPDKVKEYKNGKKGNLN
                     LFIGEVMKLSRGAADPKTTTQLIVQLLK"
     gene            13017..14156
                     /locus_tag="PLL59_04565"
     CDS             13017..14156
                     /locus_tag="PLL59_04565"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMY23111.1"
                     /translation="MRKLLNTSLLIIIASLIFVSCKHFNGYTIQGTIQNANGQKVFLE
                     DITTEVPAVIDTTSVVNNSFEIKNYSSKGIYRLRIGDDPRKSIFLYILEKDKIQVKAD
                     YNNIEKYSVEGSKGSASIQDLQKNAASYFMALDGIVAQIKSNENAKDSLIPIFNSKKT
                     EYVKYIKSFVEKEEVNDVACFALGFLGDFMQEEIPYLIDEVEKLHKAEPDSKYIANWY
                     QQTEQYKAAAMAQNEGGIAINTQAPNIILENPNGDTIQLKNLQGNYVLLDFWASWCGP
                     CRQENPNVVALYNKYHAKGFDIFSVSLDSRKDKWIEAIQKDKLAWKNHGSDLGGWSSA
                     PAQLYQVQSIPQTYLLDKTGIIIAKNLRGKQLENKLAEIFAAPIN"
     gene            14393..16102
                     /gene="ilvB"
                     /locus_tag="PLL59_04570"
     CDS             14393..16102
                     /gene="ilvB"
                     /locus_tag="PLL59_04570"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HMY23112.1"
                     /translation="MSNADKKLISGSEAVIKSLVEEGIDVIYGYPGGAIMPIYDALWH
                     YMDKINHVLTRHEQGATHAAEGWARVKGKAAVCFATSGPGATNLVTGIADAIMDSTPM
                     VCITGQVPMHLLGSDAFQETDVVGITMPITKWNYQITKAAEIPSILAKAFYIANSGKP
                     GPVLIDITKNAQFEMMEWQGYPTCTDVRGYKPKLTANIEQLKQAAELINTAKQPLIIA
                     GQGVIISDAKEILEQLSSKTGIPVATTMQGLGGFNPAHQNYVGIPGMHGNYSVNIKMN
                     ECDVMIAIGMRFDDRVTGDVTKFAKQAKIIHIEIDPVEIDKVVKTSVGICADANEALT
                     AILPLVTDNKHDAWIQSFKDLDKLEQEKVIQNAIHPNSQGIKMGEIMHLIDEKTKGEA
                     IIVPDVGQHQMYAMRYYNYRKPNSWVSSGGLGTMGFALPAAIGAQMAAPDRTVVAIIG
                     DGCFQMTVQELGTIWQNELNIKVVIFNNNYLGMVRQWQQLFFDNRYSQVALKNPDFVA
                     ISKGFRLEAAKVEKREELSAALDKMFAHKGGYVLEVVVEKEENLFPMVPSGKACSDVI
                     LEP"
     gene            16113..16352
                     /locus_tag="PLL59_04575"
     CDS             16113..16352
                     /locus_tag="PLL59_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23113.1"
                     /translation="MKAYKIQFTSYNQPVLIGRFMLIFSRRRVDVTNFFFNKIDENDG
                     QFIIEFNAEDWAAEKLLKQVAKQIDVFEAQLIINS"
     gene            16403..17446
                     /gene="ilvC"
                     /locus_tag="PLL59_04580"
     CDS             16403..17446
                     /gene="ilvC"
                     /locus_tag="PLL59_04580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HMY23114.1"
                     /translation="MAVMNFGGTEENVVTREEFPLAKAKEVLANETIAVIGYGVQGPG
                     QALNLRDNGFNVIVGQRRPSKSWEKAEKDGFVAGQTLFEPEEALEKGTIIMYLLSDAA
                     QIEMWPTVKKHLTTGKALYFSHGFGVTYKERTGIVPPADVDVILTAPKGSGTSLRRLF
                     LEGKGLNSSYAIFQDATGKAYDRTIALGIGIGSGYLFETTFQKEVYSDLTGERGVLMG
                     ALAGIMEAQYSVLRKNGHSPSEAFNETVEELTESLIKLVSENGMDWMYGNCSTTAQRG
                     ALDWKGPFREAVLPVFNELYAKVAAGEEAQRSIDTNSQPDYREKLEAELAEIRNSEMW
                     QTGKVVRSLRPGK"
     gene            17537..18031
                     /locus_tag="PLL59_04585"
     CDS             17537..18031
                     /locus_tag="PLL59_04585"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HMY23115.1"
                     /translation="MEITLTTPAILFSTVSLLYVAYTTRFVAISNLIRNLKTRFENGH
                     EEAVLQQIKNLKVRVLLIRNMQLSGIIALIVSSFSMVFIYVGKQDIGFIFFGISLLFL
                     IYSMLLAAREVLISVHSLSIELDSIEELKNAEIDDASLIAHNFKKIKKVTKDLFEQEE
                     IEKK"
     gene            18269..21376
                     /locus_tag="PLL59_04590"
     CDS             18269..21376
                     /locus_tag="PLL59_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY23116.1"
                     /translation="MRKISLLIFITYAFSQIAFSQATFKQTDILKSYKEAEELVNLGK
                     FAVAQPILQDFLQQYEQKTLDKSNMIYADAIFLKALCEKETASPEAEKDLQYFADNFK
                     GHPKTNSAYFHLGDIAFGKANYNDALAFFEKVDEKSLTGKDVTEFEYKRAFAHFALKK
                     FSQARMYFNLITLNKKHPYHEDAYYYSGLSSYYLKDYNAAYKSFQPLEVSKKYAKIVP
                     YYITSIKFINKDYKGVVEYAEPKLKEKVSYQNEMMHLLGNAYYELKNYDKALFYLEQY
                     TQKSAKVTPEDYYILGYVQAQNGKCDKAIDNLKELSPLQTALSQNAMYLLGQCYEKSG
                     NKSEARTAFLQAARMSFDKVIQEEAWFSYAKLSYELGFTNDALVALKNFIQAYPKSTH
                     TLEANELLADLFLMTRNYEEAMSIIDNLPIKSDKLKEAYQKMAYFRAVENYNDYKYDA
                     AESFFDKSLKYPISKSLEALCYYWKADIAHQKSAYDKSISGISKFLPMAYAVNTIDSD
                     KVNAGTANYLQGYNFYKKKDYANSVSYFSKAVSSLRTDANITNKNNILPDALIRLADG
                     YFMQKQYKQSAQYYEEVIKSNALGADYALYQKSIIDGLGGNYNDKIAGMKALVARFPN
                     SAFADDAIMQTGNTYMAQEKDDLAIETYKSLITKNPKSDQIPEAYLKLGLIYFNKDSY
                     DESLTWYQKVVHTYPNTPTSNEAMLAIKEIYIAKGDPNGYIAFANKYPGSKVTVSEQD
                     SIIYLAAEKQFFNGNMESALQGFSNYLSQFPNGYFALQANFYKSECLFTKQDFSNALK
                     GYEYVISQAQNRFSERSTARAANINYYDLRNYVRSYELYTKLQTIASLDENKREAVIG
                     LMRTSFYLKKYQECIDYASKVAVLPALPEFYKAEIAYYKGMCLFQQKDYDKSIKELEY
                     VVKNANNEQAAESKYTVSKMYYLKGNKKQAEKECNDYLDKFPSYEYYLGKTFILLSDI
                     YKDDKKLLQAKATLQSLLDNYSQDDDVKAEAQQKLDVILASEMEGSKLKLPDNSTQMQ
                     FDSGK"
     gene            21423..23126
                     /locus_tag="PLL59_04595"
     CDS             21423..23126
                     /locus_tag="PLL59_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23117.1"
                     /translation="MKKIQHIITVFLSVIALHSMSQSNVDTLPQLDEVDIVKAYEPIL
                     ISSNKVPFSPNLPNLSKAKPDAQTYNYTDVKGNVTYGPEDIKPIKSAGKKPEKNQFFY
                     AKIGAGFPITPLVKLIITNPVKTQYSAGLDLDFIMTFKNKKEYQNYTDLKVKGFGEYY
                     VKKKAAIGAELYYRLNQHKFYGYANDVIFDKDSLKTNFNRFGGAIKLRGIEDANYYYN
                     GDVNFASVINKNVFHKPKEITVGVNAEGGYNFKKNYWLGAKLMIANVHYKDNYQDTSF
                     FAFLNNQNHFTIQAIPYGKIKFKIWQLKAGPNLIVTNRKFYVLPEIENQLQIYKDYFV
                     MYNEWKTQVKINSLHNLSLDNPFIVTDNYDNAIDEIRTIAGFRGSVKGFGYDVRFSQL
                     VSRNQAQYVNEIFQTTTDTLPQATFNLQNVNIIKAWNPHFALSYNKGSQFGAKAWFDY
                     FIYNKNTAAQLSYLPKLKAGFSAFYNWNDKLYVSLDVTGHGKVNAVQHTYDDTLAQTT
                     TLIPIKGLVDVNLSANYFITKNIGVFVDLNNLGFQKWQRFYRYPSYNFQVIAGVKLSF
                     K"
     gene            23319..24308
                     /locus_tag="PLL59_04600"
     CDS             23319..24308
                     /locus_tag="PLL59_04600"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23118.1"
                     /translation="MVALIQQLLYTNNCVIIPKFGAFIGNYEPATIHLQENKVLPPSK
                     LIAFNRSLQKNDGLLLHYVMEKNAIDYHEAELMINSFSEKCNKSLVENRSLLLAGIGI
                     FKLDTENNIQFQPYHTTNYNLESFGLPTLSIYAIQRIKDNEAQIKSNYQRKLHPEYNT
                     SIPAKSKTKKWTFWMSAAVVLAAIAITSIQVASQNKVVEQNQSSLLPSLSNTPTLEPV
                     ATPTVTTSVVSETTVQETTEATANEVPSTSSLPVSKSYIVIGTYFDEARAGKLKSEVE
                     AKGYTAVVSKDTENGIFRTTVQVASNDVEHALQQIKSDINTRAWIFCIKCNLN"
     gene            24347..>24915
                     /locus_tag="PLL59_04605"
     CDS             24347..>24915
                     /locus_tag="PLL59_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMY23119.1"
                     /translation="MISCKNIQKSYDQLQVVKGVSFDIQKGELACIVGPSGAGKSTLL
                     HIIGTLDKADAGSISINNQEISSLKAAELSAFRNKYLGFVFQFHHLLPEFTALENVCI
                     PAFIAKKSESETLKRAKELLDFLGLKDRLQHKPNELSGGEQQRVSVARALMNQPLLIL
                     ADEPTGNLDTERSEELHQLFFDLRNEFKQT"
BASE COUNT         8833 a         3932 c         4319 g         7831 t
ORIGIN      
        1 tatttgaaag ttttggatga aatgcttgaa ggttataatc gccaaattct ttcactaatg
       61 taaaaccagt attgtttaaa aatgtatgaa aatggtgttg ctttaaagct tgtacttctt
      121 ctttaaacat ccacatgtta tcaccttttt ctatttgtat atgctttata attttgccat
      181 ttacgacttc tcttcgtata taaaaatgat gttcatcaat gattatgttt tcgcgtggaa
      241 ctaaattgtt gatgactttt tctgcattca taaaatcaat aagaatgtat cctccatctt
      301 tcaggcctaa gtgcatactt tgaaatactt tttcattttc ttttatatct gaaaaatatc
      361 cgatactggt gaaaaaattg aaaatataat caaagtagtt tatgcgaaat ggcaaacgca
      421 tatcatgtac ataaaattct aaattaggaa gttggtacaa tgtattagca tagttgatgc
      481 tttctttcgc taaatctaca ccaacgacat taaaaccttt ttcggctaag taatgtgcat
      541 gtctgccttt tccacaagct aaatctaaga tgttgccata gtcgatttgc aaatgattga
      601 tgacattatc aatgaaaaat gctgcttctt cgttattcct atttttatat aataaatgat
      661 ataaagggga atcaaaccaa acctcaaacc attgtttttg ttgctccgac atacataaat
      721 attcgattta caaattaagc atttcgattt ggaataaaaa ataatagaag ttattctttt
      781 taaaaaatgt atttatttcg aaatggagtc gatattttta atatattttc tccatattcc
      841 tgtgctccag taggtagttg taaataccaa taatgtaagc caatatacga cttcgcagcc
      901 ccaacaaata tataatggac tttgttttat ttgtacaaaa taatagcaat acagcacata
      961 aaataaacag caaaatattt ctacaatagt gggtataaaa gttgcgcctg tgccagtgat
     1021 acctaaattc attatcattc cagatgaaaa aaataataat gcaatcatta gtgtgtaaaa
     1081 aggtgcgatt gtgtcttgaa ttaattgtgt atcatttgta aaaatggata atatttgatg
     1141 tggaaaaagg acaataatta gatttatact taatgaaatt ccaacagaga atgtcaatac
     1201 tttttttaat gctggcacca catcgtcgat tctgccttgt cctatgatat tgctgatgag
     1261 tgtgttgctt gtgctggcta aactccacac gggaataccg ataaacacga ataaactttt
     1321 taaaattccg gatattgcta attcgtgctc gcctattttt tctatagata gaaagaaata
     1381 ccaccaagaa ccgatgccga ttaaattttg taaaatgata ggcgtagaga gtctgataat
     1441 tgtataaaat tgtttaaaat aaatctgctt aaatgtaaat gcgttgaatt ttttatgatg
     1501 ttttctatag agaaaataac ataagtacac taaaaacaag acggtgtcgg caattgctgt
     1561 tgcaatgcct gcgcctttta tgcccattgg agaaaatccg aagttgccaa agataaaaat
     1621 atagccacaa atgatattgg tgatggccat gaagacagta gctaaagtaa taacttttgt
     1681 tttgcctatt ccagtgtaaa atccattaaa acaaaagcct aaaacaaccg gaattactgc
     1741 gtaattcatc atattgatat aagccatggc tgactcttgt attgctacgg atttgactac
     1801 aaatgctaat acataaggcg tggcatattt aaaaaacaac attaaaaata cagataaaat
     1861 aagtgcaatt aaaattaaat gatcaaatgc aactccaatt ttatcgtctc ttttttcacc
     1921 gctatatcgt gctaatatta cttgacaacc acgtgctaat ccgccggcaa taaatacaaa
     1981 aacgaaatag taaatacctg caagcgtaat cgattctaat gctacttttg atgaatgccc
     2041 taaaaaagca gtgtcaatta attgattgat ggaatgtgct aaacttccta aaataatagg
     2101 ataggagatt tgccaaattt cttgataggt gccttttaac tgcataatat cttaatttgc
     2161 tattatgaat attttttaga cataataaca atatatagtt cttttgtttg taatgtttta
     2221 cgtcagtgat tcactccaca ttttccattg gtcgccccag agtctgttgc cagcatccac
     2281 gtataatgtt tcgccactaa tgtaggatgc cataggcgat gacaaaaata aaacaggata
     2341 agctacttca tcgactgtgc ccattctttg gttaggtatg cttttttcga tgccttttat
     2401 tatagctggc ggataagtgt ccattcctga tgattgaata attccaggcg ctatagcatt
     2461 gacgcgaata ttatatcgaa cccattcaat ggctaatgtt ttagtcatat tgtcaactcc
     2521 agcacgagca gctcctgtgt gcaccatgcc aggaaatcct ctgaagatat tagcaataat
     2581 attgacaata ttgccatctt tttgaggaat gaaaaatgtt ttcgccataa tttgtgtgac
     2641 atagaaagtg ccaattaaat tattggtgac aacggcacgg aaaccgttgt acgatatgtt
     2701 ttctgctgcc gatggaaact gcccacctgc attgtttact aaaatatcta atctgccaac
     2761 ttcttctttt atttgattag ctagattttc tatttgtgcg gtttcacgta tgtcggcaat
     2821 tgcacttctt acatcaccaa tttcttttaa ttccgtaatg gctttcgcaa ttttttcagc
     2881 attcctagaa gcgatataga ccgttgcacc taatgctaaa tattgttttg caatggcata
     2941 accaatgcca cttccaccgc cagtaactaa aactattttt cctttaaata aatcattctg
     3001 aaacatacat ttttttttat acgactaaaa taatcaaatc ttattagcta gtgtgtgttt
     3061 gatataaata ttctttttta atgaaccttt taatttgtta caaggtttac aaaaatatta
     3121 caagttattc tactacttta ttattccctt taatactaat cattaaaaca ccagctaaca
     3181 ctaataaagt acctatcaaa tgtaaaaatg taaattgctc ttgtagtatg taataagata
     3241 ggaatattgt tgcaattggt ccaatactgg caacaattgc catatttgat gagcctatca
     3301 attgaatgcc ttttgacatt aaaaaagatg gcaatacagt gctgatgatg gcaatcgcaa
     3361 gaccaataaa atatacttct ttggcgaaat taaaaatatc aagaccatga ataaaagtaa
     3421 aatggaataa tacagccaag catgatacaa tcatcgaaat acaggtaaat cgaatagtgc
     3481 caatgttatt tatcaattta tcgcttttta ctaaatataa agcataggtt agcgcactta
     3541 atagaatgta aattgttcct tttgttaagt tcgaagatac ataagctgct ttgtcggaat
     3601 aaaaagccaa tagaatgcct atatagcaaa tgataacagc agtaatttgt atgttactta
     3661 ttttcctttt gaagaatatt tttgacatca acaatacaaa agtcggatag gtgaaaatga
     3721 tatttcgttc taaagaagcg ctcacatatt gtaagcctaa aaaatcgaat aatgctgcta
     3781 agtaataacc aattaatcct aataaaatta catctgacca atctctgcgt tttatagctt
     3841 tcttttcttt acgcatttca tgtaatagaa tggcaacgta aaacggcaat gcaaacaaca
     3901 tgcgcaacat caatatttgc aagccatcaa cattatatcg aaatgctaat tttgcgaata
     3961 tgcctttcgt tgagaagcag attgcaccta agaaaacaaa aaatattcct ttgtaaaatg
     4021 gagatttcat atttatataa aaaaaggcag ccataaaggc tgcctgtaaa ggtaagctta
     4081 tattgtatta tttcattaag aaagatgatt gaccaatcaa aaatcctttt tcataaattt
     4141 ctacagaata ttttcctggt gcaaatttga aagtttgtgc ccagtaagaa catactgttt
     4201 tagattcatt ttgatagtca ggtgaaatgc tataagtaaa tggaatttca ttgcctgtag
     4261 tagcttcttt gaatgtgcca cttcctaaat tttgcatttg gatagtggtg ccatcaggag
     4321 agataattct aacttcaata gcagtttgtc cacttggtgc aattttattt tgtaataaat
     4381 caaaacatac acgcaacttt tctgcatctt ttgctttgct tacttcatct tcttttcctt
     4441 ttttcgttat acgaatagga gttgattgaa tatttgcagt gcttaaaata gacgctttgt
     4501 ctattctgtc tttcattttt tgattttctt cgtctaactc ttgtttgcgt tgtgtagtcg
     4561 tagtaagttc gttgcctaag ctatcacgct cttggtataa ggctttatta acagtgagta
     4621 atgaatcgat agacgcttgt aagtgcgctt tgtcactttc catttgcgct attaatgctt
     4681 gtgcatcagc caataatttc gatttagtgg catcgcctga ttttaatgta gaaatctgac
     4741 ctaacaaagc atcaattttt gcttttttag cgctcaattc ttgttctttt acagtcaata
     4801 agctgtctaa tccagcattt tcagcacggt agctctcaat ttcttgtaaa gactctgcat
     4861 actttttgtc taattcttgt ttcaacgttg ttacatcatc acgttgtttt tcagtaacga
     4921 caatagtctc ttttgatttt tttaatttta aattagtata tactaatcca ccaatcaata
     4981 aggcaattag taaaatgtac atagcatgta ccttgctgtt ggagtttgaa cctccgtttt
     5041 ctaattcgct cataagtgtt tttttttgtt tacgttttgc aaatttacta taaaattcct
     5101 tacacaatat acacaaaacg ttatatattg tgaataattc atctgtcaat tcaacacaat
     5161 tctaaagata tgctcgaaaa tattaaactt actgatgtgc tctttatcga tatagaaaca
     5221 gttcccaatg tgtctgattt taataactta acgccatacg agcaagaatt atgggaagag
     5281 aaaaaaggca aacaaaggag tgaagatgag tctgttgcca attactattt taataatgct
     5341 gcaatctttg ctgagtttgg taaaataatt tgtatttctg ttggtcgtat cacagcagaa
     5401 ggtatgtttc aagagtttac gttgcgctct ttttatggtg ataatgaggt gaaaatactt
     5461 actgatttta ctagcttttt acaagaacaa ttcaaaagca atcgcaatat tacattgtgt
     5521 ggacataata tacgagagtt tgatgtgcct tatatttgtc gccgattaat catgaataat
     5581 ttaaaattgc cagcatattt tgaaagttta caaatgaaaa aaccttggga agctggtttg
     5641 ctcgattcga tggatttttg gaagtttggt gattataaaa actatatttc actaaaatta
     5701 ttagcatttt gcttaggcat accttcgcca aaagatgata tttcgggaaa agatgttgga
     5761 aaagtgtatt ggcaagagca aggattggaa cgaatcaaga cctattgcca aaaagatgtg
     5821 cttacagttg cacaaatagt tttaaaattg aaaggaatat ccttaatttc ggagtcgaat
     5881 attagaatag taaattaata atcagttttc atttgttatt caaataataa tcaacttctt
     5941 ctaaaaatta aaaatacaac atggcggaat tactcttaga tgtgaaaaac ttggtgactg
     6001 aattcaacac tgaaaatggt atagttaagg ctgtcgatga tgtttccttt caagtcaata
     6061 aaggagaagt tattggtatt gttggcgaat caggctctgg taaatcggtt acctctcttt
     6121 ctatcatgcg tttaattcct aatccaccag gtaaaattac aggcggacaa attaattatt
     6181 atcgcgatgg aaaaacagct attgatttag tgaaaatacc agataatgaa atgcgcgctt
     6241 atcgaggaaa tgaaatttct atgattttcc aagaaccaat gacctcatta aatccagtat
     6301 ttacttgtgg caatcaggtg atggaagcca ttatgttgca tcagaaaatt tcaaaagatg
     6361 aagcggagaa aaagacactt gaattattcg cacaagtaaa attaccagat cctaaacgcg
     6421 ctttattagc atatcctcat caattgtcag gtggacaaaa acaacgtgtc atgattgcta
     6481 tggcaatgag ttgtcaacct aaaattttaa ttgctgacga acctacaacg gcattagatg
     6541 ttaccgttca gaaaactatt cttgaactta tcaatgattt gagtaagcaa acaggcatgt
     6601 caaccatttt tatcacacac gatttaggtg taatcgccga aattgctgac agagtggtgg
     6661 taatgtataa aggtaaaatt gtggaacaag gttctgttgt cgatattttt tcaaatccaa
     6721 aacatccata tacaaaagga ttattagctt gtcgtccacc gcttgaaaaa cgtctttcta
     6781 aattgccggt catcgcagat tttatggaag aaaaagatgg cgctattttg gaaaaagcag
     6841 cagatatgca tgcattggta gatgcgtttg tagtaagcca acaacaaacg gaaaaaagat
     6901 tcaatgattt acaacaacat gcacctattc tgcaattgaa aaatattcaa acatggtttc
     6961 ccaataaaac caatatgttt ggtaaaccac ttgattatgt aaaggcagtt gacgatgtgt
     7021 cattcgatgt acatgaagga gaaacattgg gcttagtagg tgagagtggt tgtggtaaaa
     7081 caacattagg aagaacaata ttacgcttaa ttgaaccaac aggaggcaaa gcaatttttg
     7141 acggaaaaga tatttttaat cttggccaga aagacttaaa agaattgcgc gaacacatgc
     7201 aaattatttt ccaagatcca tattcttcac taaatccgag aatgacaatt ggaaatgcaa
     7261 ttttggaacc aatgcaagtg cacaataaat atagcaatga cgctattcgc aaagacaaag
     7321 tgatcgaatt gctggaaaag gtaaatatga aaccagagca tttccaacgc tatccacatg
     7381 agttttctgg tggccaacgt cagcgtattt gtatcgctcg cgccttggct ttgaatccaa
     7441 aatttatcat ttgcgacgaa tccgtatctg ccttggacgt ttcagtgcaa gcacaagtat
     7501 taaacttatt aattcaatta cgagaagaat ttaagtttac ctatattttc atttcacatg
     7561 acttatccgt cgtgaaattt atgagcgata gaatggttgt aatgaataaa ggaaagattg
     7621 aagaaatggg aattgcagat gaaatttaca acaatccgca acaagaatat acgaagaaat
     7681 tgattgctgc tattccgaaa ggaaatttag cagatataaa agctcgtttt gaacgagtat
     7741 aactttttac gttaaaaaac gcgtcaacat tacttaaaac aacacacttt taatttgtgt
     7801 gtcaattcat tacctttgcc gaatgaaaag tagtttaaca gcttcagaaa ttcgcgctgc
     7861 atttttagat ttttttaaaa gcaaacaaca tgaaattgtt gcttccgcac ctttggtggt
     7921 gaaaaatgac cctaccttgt tattcattaa ctcaggtatg gcaccattta aagatttttt
     7981 cttaggcatc ggaaagccaa gccatccacg tattgctgat actcagaaat gcttgcgcgt
     8041 gtccggaaaa cataatgatt tagaagaagt aggcgttgat acttaccatc atactatgtt
     8101 cgaaatgttg ggcaattgga gctttggaga ctactttaaa aaagaagcga ttgcttggag
     8161 ctgggaattg ttgacagaag ttttaaaact tgaaaaagat agattatatg taaccatctt
     8221 tgaaggaaat gaagctgaaa atattccaaa agatatcgaa gcgtataatg aatggaaaaa
     8281 agtaattgct gaagacagaa ttttattagg taataagaaa gataatttct gggaaatggg
     8341 cgacacgggt ccttgcggtc catgtacgga aatacatgtt gattgccgtt cagatgtaga
     8401 acgaaaacta ttgatggcgc taccttggtc aatgcagatc atccacaagt aatagaaatt
     8461 tggaataatg tatttatcca atttaataga aaaaaagatg gaagcctcga acaattgcct
     8521 gcaaagcatg tcgatacagg catgggcttt gaacgattag tgcgcgtact tcaaggcaag
     8581 tcttccaatt atgatacaga tgtgttttct ggcacaattc aaagtacaga aaatattgtc
     8641 gggaaaaaat atgatagaag cgatagcaag gaagccattg cttttcgtgt gattgcagat
     8701 catattcgcg ccgttgcatt cactattgca gatggccaat tgccaagcaa tactggtgca
     8761 ggctatgtaa ttcgccgcat tttaagaaga gctgtaagat attatttctc ttatttagat
     8821 aaaaaagaac cacttttgca tttgttagtg ccagtgttgg caacacaatt tgaaaatgtt
     8881 tttcctgaac tgaagaaaca agaattattc gtagctaaag taattttaga agaggaaaat
     8941 gctttcttga aaacattaga taatggtctt aaaaggttag ataatattat caaagaatca
     9001 gataaaataa tagaaggaaa agcagcattc gaattgtttg atacatatgg ttttccaatc
     9061 gatttgacga atttgattgc ggctgaaaaa ggtctttctg tggacgaaaa tgaatttgaa
     9121 tcagagttac aaaaacaaaa agaacgcagt agagcagcaa cagaattaaa tgtcgatgat
     9181 tggattacat tgagtgatgg tcaatcaata tttgttggct atgctagctt ggaaacaaaa
     9241 gcaaatgttt taaaatatag aaaaattaaa gccaaaggaa aggaacaata tcaacttgtt
     9301 ttagataaaa ctcctttcta tgcagaaagt ggtggtcagg ttggcgatgt gggtgtttta
     9361 caatttaaag acagcaaaat actcatcaca gatactaaaa aagaaaatga tttaattgta
     9421 cattttacag atgagattcc tgctgatatg agcggagaaa ttatagcaaa tgtaaatgtc
     9481 gaaatacgta aaaaaactac tattcatcat tctgctacgc atttattaca agcagcatta
     9541 caacaagttt taggaaagca tatcgcgcaa aaaggttctt taaataatgc agaatattta
     9601 cgattcgatt tttctcattt ctcaaaaatg acaaatgaag aattggaaaa tgtagagcaa
     9661 ttagtaaacg caaaaattcg tgagaatatt ccagtggtaa ttaaagaaat gccaaaagag
     9721 gaagcaacaa aattaggtgc tatggcactg tttggggaaa aatatggcga tgttgttcgt
     9781 gttgtcatta ttgatgaaaa atatagtgtc gaactttgtg gtggcacaca tgttggagca
     9841 actggcgaat taggattgtt taagataaca tcggaaagtg cagtagctgc tggtgttcgc
     9901 cgtattgaag ctgtgtgtgg cgaagctgca cttgcttatg taaatacaaa aatatctgat
     9961 ttgaatgcta tcaaagaagc attaaaaaat ccgaaagatg ctgtcaaatc aatagaagtt
    10021 ttacaagatg aaaatgcttc actgaaaaaa gaagtagaaa aatatcaaaa cgaaaaagtt
    10081 gaaattatca aacaacaatt gcgctcaaaa gtaacaaata gaaatggtgc aaatgtactt
    10141 tttgaaaagg tagttggcgt aaatgcagat agcttaaaga aaatccaatt tgatcttaaa
    10201 aatgaatttg aaaacttatt cttcgtagcc agtacgattg taaatgaaaa accattactg
    10261 tctattatta tttctgatga tttagtgaaa tcaaataatt tacatgcagg caatatggtt
    10321 aaagaattag ctaaagcaat aaaaggcggc ggtggcggac aggctttcta tgctactgct
    10381 ggtggcaatg atgctagcgg aattgatacc gttttaaagc aagctgaaga acgcttttta
    10441 atacttacca gttaattcaa tttcagagtt cataatatgt caaattaaaa gtgctactcg
    10501 taagagcagc acttaatgaa tgttatgtaa ccaaataaat aaaaaaccaa atattaataa
    10561 tacgtgtaag tcgtaacatt agtaatcaaa ccttttgttt ccgtcttttt aattactcgc
    10621 tgatttgcat caaactcata agtaaataca gcggtttcac catcttctat tgaagtcatc
    10681 agtaaattag ttgaactttt tcctaaaaat gattttccaa aatttctatt gccaatgcta
    10741 tttattttat ctgaataata aatgaacgat tgattgattg taaaatttcc acctacataa
    10801 gaaaatcctt taccaatcat tttttctaca tttttaccat cgtttgtgta atcttcatta
    10861 atagaataat cttgaattcc taatgattgc ttgcgcataa atccagcatc atcatattcc
    10921 atattttgtg tttggtaaaa tgaattcaaa ccaacataat aaccttgttt attcaattta
    10981 tatactatcg gattagaatt tctattgtat ttcgtagcac cacttggcaa ggtttcttct
    11041 aatgcataac gcggttcttc gctcttatac tcttgtattt catttgatga atacgtaaac
    11101 tcatggaaat acgaagtcga gccagtattg aataaaattt tcgaaattcg tttttgacta
    11161 tcgtaataat aggtttccgt tatgcctttt tctgttttgg tttttaccaa agcatcttca
    11221 ggtgatgatg gtgcatcagt caatcgttgc tgttcctttt cacatgacat aaaaagaatg
    11281 cacactaata ccgttagtat ataatattta ctttttaaca taaaaaccca ggacaaatat
    11341 actaaacaac gcctattatt gcccaaatat tatgacataa atcaattttt taacataccc
    11401 atttttataa gtcaatgata atcagttgtt tgttattgtg ttattaaatt ttaaatcagg
    11461 taaattgtac tatattttct tttatttcac atgtcaatta tattcttaga atcttagcgt
    11521 atctttgtaa atttttaaaa atggaagata aatacgaaat agtaataggc ttagaagtac
    11581 acgctcaact caatacaaaa acgaagattt ttgctgcaga tgaaacgact tttggcgcag
    11641 caccaaatac gcaaataagt ccaattactg taggtttacc aggaacgctt ccaagattaa
    11701 ataaagaggt ggttgaattg gctgttaaat taggaattgc cttagactgc catataaatg
    11761 aagtcaattt ctttgatcgt aaaaattatt tttatgcaga cctgccaaaa ggctatcaaa
    11821 tttctcaaga tagacagcca atttgtgtag gtggttttgt ggaaattgtt gtcaataatg
    11881 agccaaagaa aattgcatta tccagaatac acatggaaga agatgctggc aaaaacaacc
    11941 atgaacttga tccagatttt tctttgattg atttaaatcg tgctggtgtg cctttacttg
    12001 aaattgtttc agagccagat attcgtagta gcgatgaagc ctacgcttat gtgacagaat
    12061 tacgcaaatt ggttcgatat cttgatgtct gtgatggtaa tatggaagaa ggttctatgc
    12121 gttgtgatgc caatgtttct gtgcgtttaa aaggagaaac aaactatcgc aaccgcgtag
    12181 aagtgaagaa tatgaattct attcgaaacg taaagcgtgc aattgatgca gaagtaaaac
    12241 gacaaattga aatttatgaa aatggtggaa cagttgatca ggaaacacga agttttgatc
    12301 ctgttgatgc cagctctttc accttgcgct ccaaagaaaa tgcacatgat tatcgttatt
    12361 ttccagaacc agatttaccg ccagttgtta tttcatcttc aacaattgag caaataaaat
    12421 cacaaatgcc acaattgccg aaagccttgc aaacgctgtt cactcaaaca tataatttga
    12481 ctgcatacga tgcacaagtg ctgactgatg ataaagaaac agctgattat tttatggcag
    12541 ttattcaata tacttcaaac tataaagcag ccgcaaactg gattaataat gatgtaaaat
    12601 cattgttgaa tgaaattaat tgttcgctaa aagattttgc agtgcaagcc aaacaactag
    12661 cagaaataat tcaactttta gatgataata ttgtaagctc atctggtgct aaaactattt
    12721 ttaaagcatt tgtagatgga aacaaatcat cggcaattgc ggtggcagaa gcgttgaatt
    12781 taattcaatc aagcgataaa gatgaattaa caaaatggat tgaggcagcg ttagcaaaat
    12841 atcctgataa agttaaagaa tataaaaacg gcaaaaaagg aaatttgaat ttgtttatag
    12901 gtgaagtcat gaaattatca agaggcgctg cagatccaaa aacaacaaca caattaattg
    12961 tacaattatt aaaataacaa ttacacattt tctgtgtttc caataaaaga ataaaaatga
    13021 gaaaattatt aaacacaagc ttactaatta ttattgcaag ccttattttc gtttcttgta
    13081 aacatttcaa tggttatacg attcaaggaa ctattcaaaa tgcaaacgga caaaaagtat
    13141 tcctagaaga cattactact gaagtgcctg ctgtaattga cacaacaagt gttgttaata
    13201 attcatttga gattaaaaac tattcctcaa aaggaattta tcgtttgcgc attggcgacg
    13261 atccaagaaa gtctatcttt ttatacattt tagaaaaaga taaaattcaa gtaaaagcgg
    13321 attataataa tattgaaaaa tatagtgtgg aaggaagtaa aggctcagca tctattcagg
    13381 atttgcaaaa aaatgccgca tcttacttta tggcattaga tggcattgta gcacaaatta
    13441 aatcgaatga aaatgcaaaa gattcattga ttccaatttt taattcaaag aaaacggaat
    13501 atgtaaaata tataaaatca tttgtcgaaa aagaagaagt aaacgacgtt gcatgcttcg
    13561 cattgggctt tttaggcgat tttatgcaag aagaaattcc atatcttatt gacgaagttg
    13621 aaaaattaca taaagcagaa cctgattcta aatatatagc caattggtat caacagacag
    13681 aacaatataa agctgccgca atggcacaaa atgaaggagg cattgccata aatacgcaag
    13741 caccaaatat catattagag aatccgaatg gcgatacaat acaactaaaa aatttgcaag
    13801 gcaattatgt attactcgat ttttgggcgt cttggtgtgg tccttgccgt caagaaaatc
    13861 caaatgttgt agcattgtat aataaatatc atgctaaagg atttgatatt ttttctgttt
    13921 cactagattc gagaaaagac aaatggatag aagccattca aaaagataaa ttggcttgga
    13981 aaaatcatgg aagcgactta ggtggctgga gctctgcacc agctcaatta tatcaagtgc
    14041 agtcaattcc acaaacgtat cttttagata aaactggaat aattattgca aaaaatttac
    14101 gcggcaaaca attagaaaat aaactcgccg aaatttttgc tgcaccaatt aattaattga
    14161 ttacgacttt tattttgttt agatgaattt tttgtctaat tataacatac gtgacaaaaa
    14221 aaatatttga ctacgatttg acttttagca caaatacaag caattttaag agctaattca
    14281 attttattaa ttcaatctgt taagaaatta ctattattct acacttatca tcattctttt
    14341 ttttataagc tttcattttt attagatttg tacgtacagt attttttgaa caatgtcaaa
    14401 cgcagataaa aaattaattt caggttctga agccgttatc aagtcactgg tagaagaagg
    14461 aatcgacgtt atttacggtt atcctggtgg agccattatg ccaatttatg atgcactttg
    14521 gcattatatg gataaaataa atcatgtctt aaccagacat gaacaaggcg ccacgcacgc
    14581 agcagaaggt tgggcacgtg taaaaggaaa agcagctgtt tgttttgcta cctcaggtcc
    14641 tggtgctacc aatttagtaa caggtatcgc agatgctatt atggacagca caccgatggt
    14701 atgcattacg ggtcaagtgc ctatgcattt attaggctct gatgcttttc aagaaacaga
    14761 tgtcgttggt attaccatgc ctattacaaa atggaattat caaataacaa aagctgctga
    14821 aattccaagc atattagcta aagcttttta tattgctaac tcaggaaaac ctggtcctgt
    14881 attaatcgat atcaccaaaa acgcacagtt tgaaatgatg gaatggcaag gatatccaac
    14941 ttgtacagat gttcgcggat ataagcctaa attgactgct aatattgaac aattaaaaca
    15001 agcagctgaa ttaattaata cagctaaaca acctttaatt attgcaggtc aaggtgttat
    15061 aatttcagat gctaaagaaa ttttggaaca attgtcttct aaaacaggaa ttcctgtggc
    15121 aacgacaatg caaggtttag gcggtttcaa tccagcacat caaaattatg taggcattcc
    15181 tggtatgcat ggtaattatt ctgtaaatat caagatgaat gaatgcgacg tgatgattgc
    15241 cataggtatg cgcttcgacg atcgcgtaac tggtgatgtt acaaaatttg caaaacaagc
    15301 caaaatcatt catatagaaa ttgatcctgt agagatagat aaagtggtga aaacaagtgt
    15361 tggtatttgt gcagatgcga atgaagcatt aacagcaatt ttaccattag tgactgacaa
    15421 taaacacgat gcatggattc agtcatttaa agatttagac aaattagaac aagaaaaagt
    15481 aatacaaaat gccattcatc ctaacagcca aggcattaaa atgggtgaaa ttatgcattt
    15541 gattgacgaa aaaacaaaag gtgaggcgat tatcgttcca gatgtcggac aacatcagat
    15601 gtatgcaatg cgttattaca attatagaaa accaaattct tgggtctcaa gtggtggctt
    15661 aggtacgatg ggctttgcat tgcctgcagc tattggtgca caaatggcag cgccagacag
    15721 aacggtagtt gcaataattg gcgacggctg ttttcagatg accgttcaag aattaggaac
    15781 aatctggcaa aacgaactca acataaaagt ggttattttt aataataatt atttaggaat
    15841 ggtgcgccaa tggcagcaat tattctttga taatagatat tcacaagtgg cattgaaaaa
    15901 tcctgatttt gttgctatct caaaaggatt ccgtttggaa gccgcaaaag tagaaaaacg
    15961 cgaagaactg agcgctgctt tagacaaaat gtttgcacac aaaggtggct acgtattgga
    16021 agtagtagta gaaaaagaag agaatttatt tcctatggtg ccaagtggaa aagcttgcag
    16081 tgatgtaatt ttagaaccgt aaaaatcaaa tagtgaaagc atataaaata caatttacat
    16141 cttataatca acctgttttg attggtaggt ttatgttgat tttttcgaga agaagagtag
    16201 atgtgactaa ctttttcttt aataaaatag atgaaaatga tggtcagttt atcatcgaat
    16261 ttaatgcaga agattgggca gcagaaaaac tattgaagca agtggctaag caaatagatg
    16321 tttttgaagc tcaattaatc atcaattcct aaaaatcaaa ttccaataat tttaaacttt
    16381 aaatctttta aattaataaa aaatggcagt aatgaatttc ggcggaacag aagaaaacgt
    16441 tgtaacacgt gaagagtttc cgttagcaaa agcaaaagaa gtattagcaa atgaaacaat
    16501 agcagtaata ggctatggtg tgcaaggtcc tggacaagct ttaaaccttc gcgataatgg
    16561 ttttaacgtt atcgttggtc aacgcagacc atcaaaatct tgggaaaaag cagaaaaaga
    16621 tggctttgtt gctggtcaaa ctttattcga accagaagaa gcattagaaa aaggtactat
    16681 catcatgtat ttattatctg acgctgctca aatcgaaatg tggccaacag taaaaaaaca
    16741 cttaacaact ggaaaagctt tgtacttttc acatggtttt ggtgttactt acaaagagcg
    16801 tacaggcatt gtgccgccag ctgatgttga tgtaattctt acagcaccta aaggttctgg
    16861 tacttctcta cgtcgtcttt tcttagaagg taaaggctta aattcttctt atgctatctt
    16921 ccaagatgca acaggaaaag catatgacag aacgattgca ttaggtatcg gaattggttc
    16981 aggttattta tttgaaacaa ccttccaaaa agaagtatat tctgatttaa caggcgagcg
    17041 tggcgtgtta atgggcgcat tagcaggcat catggaagca caatacagcg tattacgtaa
    17101 aaatggacac tcaccatctg aagcattcaa cgaaacagta gaagaattga cagagtcatt
    17161 aatcaaatta gtatctgaaa atggtatgga ttggatgtat ggcaattgct ctacaactgc
    17221 acaacgtggc gcattggatt ggaaaggtcc tttccgtgaa gctgtattgc cagtattcaa
    17281 tgaattgtat gcaaaagttg ctgctggcga agaagcacaa cgctcaattg acacaaactc
    17341 acaaccagat tacagagaaa aattagaagc agaattagca gaaattagaa actctgaaat
    17401 gtggcaaaca ggtaaagttg ttcgcagctt acgtcctgga aaatagttca ttttatctaa
    17461 gttaattgaa tgccatcatt atttgatggc attttttttg aacttaaatt catattgatt
    17521 tgtttactaa tattatatgg aaattacact cactacacct gccattttat tctctacagt
    17581 ttcattgcta tatgttgctt acacaacaag atttgtcgct atttctaact tgataagaaa
    17641 tttaaaaaca cgctttgaaa atggtcacga agaagcagtt ttgcaacaga taaaaaactt
    17701 aaaagtaaga gttttattaa tcagaaatat gcaattgtca ggcatcatag cgcttattgt
    17761 tagttcgttt tcaatggttt ttatttatgt tggcaaacaa gatattgggt ttatattttt
    17821 tggcattagt ttgttgtttc tcatatattc tatgctgctc gcagcacgtg aagttttaat
    17881 ttctgtacat tctttaagta ttgaattaga ttcgatcgaa gaactaaaaa atgcagaaat
    17941 agatgatgct tccttgattg ctcataattt caaaaaaata aagaaagtta caaaagacct
    18001 ttttgaacaa gaggaaattg aaaagaaata aatcaactat tgatttgttg tagaatgttt
    18061 tttaatcaaa taaaaatgat aaattcttta tcgctattag taaagaacaa aagatatcga
    18121 attaaaatgt acacatttcc aacattagta attttttatc tccacaatag tgtataaact
    18181 tttgttttac agtcgctaaa ataattccaa ctttatgttg gaataatttt tgttattctt
    18241 cgttattaat cataattaaa gatactttat gcgtaagatt tcacttctta tatttattac
    18301 ttatgccttt tcacagattg cattttcgca agctactttt aagcagactg atattttaaa
    18361 atcctacaaa gaggcagaag aattggtcaa cttaggaaag tttgctgttg cacaacctat
    18421 tttgcaagat ttcttacaac aatacgaaca aaaaacttta gataaatcga atatgattta
    18481 tgccgatgcc attttcttaa aagcattatg cgaaaaagaa acagcatcgc cagaagcaga
    18541 aaaagatttg caatattttg cagataattt caaaggtcat ccgaaaacaa atagtgccta
    18601 ttttcattta ggtgatatcg catttggtaa agctaactac aatgatgctt tagcattctt
    18661 tgagaaagta gatgagaaat cattaacagg taaagatgtt accgagtttg aatacaaaag
    18721 agcgtttgct cattttgcct tgaaaaaatt tagtcaagct cgtatgtatt ttaatttaat
    18781 tacgctaaat aaaaaacatc cttatcatga agacgcctac tactattcag gtttgagcag
    18841 ttattattta aaagattaca atgctgccta caaaagtttt cagcctttag aagtgtctaa
    18901 aaagtatgcg aaaattgtac cttattacat tacttcaatc aaatttatca ataaagacta
    18961 taaaggtgtt gttgaatatg cagaaccaaa attaaaagaa aaagttagct atcaaaatga
    19021 aatgatgcac ttgttaggaa atgcatatta cgaattaaaa aactacgata aagcactttt
    19081 ctatttagaa caatacacac agaaatcagc aaaagtaaca ccagaagatt actatatttt
    19141 agggtatgta caagcacaaa atggcaaatg cgataaagca atagacaatc ttaaagaatt
    19201 gtcgccattg caaaccgcac tcagccaaaa cgctatgtat ttattagggc aatgctatga
    19261 aaaatcaggc aataaatctg aagctcgtac tgcattttta caagcagcac gcatgtcatt
    19321 cgataaggta attcaagaag aagcatggtt tagctacgct aaactttctt acgaattagg
    19381 atttacaaat gatgcattag tagcattgaa aaattttatt caagcctatc caaagtcaac
    19441 acatacactt gaagcaaatg aacttttagc tgatttattt ttgatgacac gcaattatga
    19501 agaagcaatg tccatcatag ataacttgcc aattaaatct gacaagttaa aagaagccta
    19561 tcaaaaaatg gcctatttca gagctgtaga aaactataac gattataaat atgacgctgc
    19621 ggaatcattt tttgataagt cattaaaata tccaatttcc aaaagcttag aggcgctttg
    19681 ttattactgg aaagcagaca ttgcacatca aaaatctgct tatgataaat ctatcagtgg
    19741 catttcaaaa tttttgccga tggcatacgc agtcaataca attgattctg ataaagtgaa
    19801 cgctggaaca gctaattatc tgcaaggata taatttctat aaaaagaaag actatgccaa
    19861 ttcggtgtct tatttctcta aagcggtatc atcattacga acggatgcaa acatcaccaa
    19921 taaaaataat atcttgccag atgcattaat tcgtttggct gatggatatt ttatgcaaaa
    19981 gcaatacaaa cagtcggcac aatactatga agaagtaata aaatcaaatg ctttaggcgc
    20041 cgattatgca ttgtatcaaa aatcaattat agatggttta ggtggcaatt ataacgataa
    20101 aattgctggt atgaaagcat tagttgcgcg ttttccaaat tcagcattcg cagatgatgc
    20161 catcatgcaa actggaaata cttacatggc gcaagaaaaa gatgacttag ccatcgaaac
    20221 ctataaatca ttgattacaa aaaatcctaa atcagaccaa ataccagaag cttacttaaa
    20281 attaggcttg atttatttta ataaagatag ctatgacgag tctttaacgt ggtaccaaaa
    20341 agtagttcac acttatccga atacaccaac atcaaacgaa gccatgttgg ccatcaaaga
    20401 aatatatatt gctaaaggtg atcccaatgg ttacattgct tttgccaata aatatccagg
    20461 ttcaaaagtt accgtatcag aacaagattc tatcatctat ttagcagcgg aaaaacaatt
    20521 ttttaatggc aatatggaaa gtgcattaca aggattttca aattatcttt cacagtttcc
    20581 gaatggctat ttcgcattgc aagccaactt ctacaaatct gagtgtttgt ttacaaaaca
    20641 agatttttca aatgcactta aaggatatga atatgtaatc agccaagcac aaaacagatt
    20701 ttctgaacgc tcaacagcac gtgctgcaaa catcaactac tacgatttgc gcaactacgt
    20761 acgctcttat gaactgtaca caaaactaca aacgattgct tctcttgatg aaaataaaag
    20821 agaagctgtt attggtttaa tgcgcacttc attttatctc aaaaaatatc aagaatgtat
    20881 tgactatgcg agtaaagtag ctgtattacc agcacttcca gagttttaca aagcagaaat
    20941 tgcttattat aaaggcatgt gtttgtttca acaaaaagac tatgataagt caattaaaga
    21001 attagaatat gtagtgaaaa atgccaataa cgaacaggcg gcagaatcaa aatatacagt
    21061 aagtaaaatg tattatttga aaggaaataa aaaacaagcg gaaaaagaat gcaacgatta
    21121 tttagataaa tttccttctt atgaatatta tttaggcaaa acgtttattc tacttagtga
    21181 tatttataaa gacgataaga agttattaca agctaaagct accttgcaaa gtttgttaga
    21241 taactattcg caagatgatg atgtgaaagc agaagcacaa caaaaattag atgtgatttt
    21301 agcatcagaa atggaaggtt caaaattaaa attaccagac aatagcactc aaatgcaatt
    21361 tgatagtggc aaataatttt tgaagaatag aaaatattat atacgcatta acagctgata
    21421 aaatgaaaaa aatacaacat ataatcaccg tttttttaag tgtcatagcc ttacattcga
    21481 tgtcgcaaag taatgttgat acactaccac aattagatga agtagatatt gtgaaggctt
    21541 atgagccaat attaatctcg tcaaataaag ttccgtttag tccgaatttg ccaaatcttt
    21601 ccaaagcaaa acctgatgcg caaacatata attatacaga tgtaaaaggt aatgttactt
    21661 atggtccaga agatattaaa ccaattaaat ctgctggcaa aaaaccagag aaaaatcaat
    21721 tcttctatgc taaaattggt gctggttttc ccatcacacc actagtgaaa ctaataataa
    21781 caaatcctgt caaaacacaa tacagtgctg gactagatct tgattttatc atgaccttta
    21841 aaaataaaaa agagtatcag aactatactg atttaaaagt gaaaggcttt ggtgagtatt
    21901 atgtcaaaaa gaaagcagca ataggagcag agctgtatta cagattgaat caacataagt
    21961 tttatggcta tgctaatgat gttatttttg ataaagattc gttgaaaaca aacttcaata
    22021 gatttggcgg agcaattaaa ttacgtggta tcgaagatgc caattattat tacaatggcg
    22081 atgtaaactt tgcctcggta ataaataaaa atgtatttca taaacctaaa gaaattacag
    22141 ttggcgttaa tgctgaaggt ggctataact tcaagaaaaa ttattggtta ggtgcaaagc
    22201 taatgattgc aaatgttcac tataaagaca actatcaaga tacttcattt tttgctttct
    22261 taaataatca aaatcatttt accattcagg caattccgta tggcaaaatc aaatttaaaa
    22321 tatggcaatt aaaagcagga cccaatttga ttgtaacaaa cagaaaattt tatgtgttgc
    22381 ctgaaattga aaaccaatta cagatttaca aagattattt tgtgatgtac aatgaatgga
    22441 aaacacaagt gaaaataaat agcttacata atttgtctct agacaatcca tttattgtta
    22501 ctgataatta tgataatgcc atcgatgaaa tacgcactat tgctggtttt cgcggaagcg
    22561 taaaaggttt tggttatgat gttcgcttca gtcagttggt ttctcgaaat caagcacaat
    22621 atgtgaatga aatctttcaa acaacaacag atactttacc acaggcaaca tttaatttgc
    22681 aaaatgtaaa tatcattaaa gcttggaatc ctcattttgc cttgtcttat aacaaaggaa
    22741 gccaattcgg cgctaaagca tggttcgatt attttattta taataaaaat accgctgcac
    22801 aattgtctta tttgccaaaa ctaaaagcag gtttttcagc attttataat tggaacgata
    22861 agttatatgt ttcactagat gtgactggtc atggaaaagt aaatgccgtt caacatacgt
    22921 acgacgatac attagcgcaa actactacat tgattcctat taaaggattg gtagatgtga
    22981 atttgtctgc aaattatttc attactaaaa atattggcgt atttgttgat ttgaataatt
    23041 taggttttca aaaatggcaa cgcttttacc gttatccaag ctataatttc caagtaattg
    23101 caggtgttaa attgagcttc aaataattgc atttgattca tgtcattctc acgaaataca
    23161 gtcattccca cgaaagtggg aatctctgtt tttctctttg ccttcctgct tccacttgtt
    23221 tatatagata gtacataagt tataggaaaa tgaaatacag caatcgattt atacacatga
    23281 tgttggatta ttgataaaaa aaattaactt tgctatgtat ggtagcatta atccaacaat
    23341 tattatatac aaacaattgt gtcatcattc caaaatttgg cgcttttatc ggtaattatg
    23401 agccggctac tattcatttg caagaaaata aagtattgcc accatcaaag ttgattgctt
    23461 ttaatcgttc tcttcaaaaa aatgatggac tgctattgca ttatgtgatg gaaaaaaatg
    23521 caatcgatta tcatgaagca gagttaatga ttaacagttt ttcggagaaa tgcaataaat
    23581 ctctagtaga aaatagaagc ctgttattgg caggcatagg tatttttaaa ttagatacgg
    23641 aaaataatat tcagtttcaa ccttaccata caacaaatta caatttagaa agtttcggct
    23701 tgcctacctt atctatttat gctattcagc gaataaaaga taatgaagca cagataaaaa
    23761 gcaactatca acgtaagtta catcctgaat ataacacatc tatacctgct aaaagcaaaa
    23821 cgaaaaaatg gactttctgg atgtctgccg cagtagtttt ggctgcaata gcaattacgt
    23881 ctattcaagt tgcttcacaa aataaggttg ttgagcaaaa tcaatcttcg ctactgccta
    23941 gtcttagcaa tacaccaaca cttgagccag tagcaacacc aacagtaaca acaagtgtag
    24001 tatcggaaac aacagtacaa gaaaccacag aagcaacagc aaacgaagtg ccatctacct
    24061 cttctttacc tgttagtaaa tcttatatag taataggtac atattttgac gaagctagag
    24121 ctggaaagct taagtcagaa gtagaagcaa aaggttatac agctgtcgta tcaaaagata
    24181 ctgaaaatgg catttttaga acaacagtac aagtcgcatc taatgatgta gaacatgcat
    24241 tacaacaaat aaagtccgac attaatactc gtgcatggat attttgtatt aaatgcaatt
    24301 taaactaaaa tcaatgcgtt atagccgttt tatttcataa acatttatga tttcctgtaa
    24361 aaatattcaa aaatcttacg atcaactgca agttgtaaaa ggtgtttctt ttgatataca
    24421 aaaaggagaa ttagcctgta ttgttggtcc ttcaggtgct gggaaatcga ctttattgca
    24481 tattattggt acgctcgata aagcagatgc aggtagtatt agcattaata atcaggaaat
    24541 ttcaagctta aaagcagcag aattatctgc atttagaaat aaatatttag gatttgtttt
    24601 tcaatttcac cacttattgc ccgaattcac tgcgctagaa aatgtatgta ttccagcatt
    24661 tattgctaaa aaaagcgaaa gtgaaacgtt aaaacgagca aaagaattat tagatttttt
    24721 aggcttaaaa gaccgcttac agcacaagcc aaatgagctg tctggtggcg agcaacaacg
    24781 tgtttctgtg gcgcgtgcgc tcatgaatca acctttacta atattggctg atgagccaac
    24841 aggaaactta gatacggaac gaagtgaaga gctacatcaa ctattttttg acttgagaaa
    24901 tgagttcaaa cagac
//
LOCUS       DAOQQV010000027        24254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_621_length_24254_cov_7.311294, whole genome shotgun sequence.
ACCESSION   DAOQQV010000027 DAOQQV010000000
VERSION     DAOQQV010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24254
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_24254_cov_7.311294"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /locus_tag="PLL59_04610"
     CDS             complement(<1..1852)
                     /locus_tag="PLL59_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMY23120.1"
                     /translation="MTKTYALFIFLIAFSISVFSKNNLQLVKIDPAILQSTSARTTTQ
                     NFRFNNVDYLTIQTINNNADYLVQQGFSIEAYLGNGYYLTSINSNVSNKINALPYSSI
                     SYLSSESKIDNSLLSTNTAQEVSVLLTSTIDENWLANILSQHGIHINNFNSKYHSFNA
                     TLNNQQIQDLSRYPFVVFISKYYSTRNPLMYESLLMTGTNQVQEVQPYGFNLKGNGVT
                     VGVWDEGAFGDHIDLPKSKNFVVDKTYSSFIPMYHPTEVGGCIASAGNLFPSIKGIAP
                     QSNIVYWDVAGDILQELETGKSTYNVDISNHSYNFVSTNCFQSGLYIPEAANLDKIVH
                     EQPTLLPIVAVGNTAAANCAIATDTFSSVDIGFQGCKNAITVGWLFGNEKLVENSGRG
                     PTEDGRLKPELVAKGFAVSALVPNDGISAVYGSSYAAPQVAGIAALLTQQYKQQFAQL
                     PNAALLKAILFNTARDLGNAGPDYMYGFGKPDAFRAVKSISNQQYFENTVNHQSIQTH
                     SFSSPANTHQLKVTLSWTDIEGSPLAQKSIVNNLDLKLVTPTGDTVLPWKLNPQSPKI
                     IALHGIDNINTNEQITIDNPLEGNYTIVVKGTDVPFGPQAYAVAYLTQERK"
     gene            complement(1929..4313)
                     /locus_tag="PLL59_04615"
     CDS             complement(1929..4313)
                     /locus_tag="PLL59_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMY23121.1"
                     /translation="MHVYPKNSLQKLDFPFILKELENLCSGSLGKALLQDLSFITNTD
                     EVNKQLHFVKELKEIIENDTQLPQYGFNTLPFLEKLQIENYFLEVKELIAVYFSLSAA
                     ADVFRFFTPKARQLLYPFLSEKVAEYQIELGIIASITKVIDVDKEIIRDNATPELSKI
                     RKDIQHKIQDINAVFRRVLSQYKQQNFLAETEETVRDGRRVLSVKAEYKRSIRGLITD
                     ESDNGSITFIEPNETLFLNNELTELYLLEKRELLKILISLTNYIRPHKTTLDKLQHLM
                     ADLDVIRAKAYFAINFNCNMPQLSNEPIIHLREFVHPVLFYHHQKQNKPIIDNAIFLD
                     AKNRIVVISGPNAGGKSIVLKSIGLIQLMLQFGMLIPAKENSIVSIFENLFVDIGDEQ
                     SIENDLSTYSSHLSNMNYFLSHASCSTLVLIDEMGMGTDPALGGPMAEAILESLHQKQ
                     VFGVVTTHFNNLKVFAANTAGMQSGAMAFDTQQLKPLYQLQLGQPGSSFTFEIARKSG
                     LPSKIIQQANLKIGDNKKALDDVLTDIQTEKHFIKGLRKNVQQKETQLQDLTNSYTQL
                     NKDLEKEKKRLLKQYEARLLERFNAESRNLENEMREWKEQKDSKEKFITVRKFIDNNR
                     ESIERKLDDAPVEKSNINNKELIVGAKVKLLEGTEIGVILSLKNNTAVVAFGSMQSTV
                     KLHQLQLIQESSKEVRISRNKFSSKILVEKSEFDNSLDIRGMLKDEAILALDNYMDRV
                     VMYGIHQLKIIHGRGTGALKQAVHFYLKKYPHVKTFRVEAEQFGGDGITIVEMK"
     gene            complement(4313..5098)
                     /locus_tag="PLL59_04620"
     CDS             complement(4313..5098)
                     /locus_tag="PLL59_04620"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HMY23122.1"
                     /translation="MQDAIQNISFIGSGNVATHLAKAFAKKGITVKQIYSQSCDNSET
                     LAWQSNSIYTCDLEELDLSVDLLIFAVKDDVIKTLSQYILKKKPDMKMVHTSGSVGLD
                     VFENATNTGIFYPLQSFSKTKKIDIEAVPFLITSNNDVFKNELMSLAKLLSDKVYEYT
                     DEQRKHLHIAAVFVNNFSNHLFAIAQNYCEKHQLDYQLLMPLIHETITKIDSILPKDA
                     QTGPARRNDVEIIDKHLTMLKEEDSQLFQIYQLLTNSILKMYS"
     gene            complement(5103..7514)
                     /gene="ccsA"
                     /locus_tag="PLL59_04625"
     CDS             complement(5103..7514)
                     /gene="ccsA"
                     /locus_tag="PLL59_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMY23123.1"
                     /translation="MNIYAIGQNLLIGKVGHFLVILSFVAALAACLAYFFATTYRNDL
                     SKSLSWKQFGRKAFVIHAVAVLGIIISLLTIIHGHYFEYKYVWEHTSKSLPWYYQFSS
                     MWSGQEGSTLLWMFWHAVLGCVLVFTAKNWESPVLSIVALAQVLLGSMLLGIILFHYK
                     FGSSPFALVRDTMDIPVFKMNPEWVAEDGTGLNPSLQNYWMVIHPPTLFLGFAGCTIP
                     AAYAIASLWVRNFTDWVKPALTWSLFTLMILGAGILLGGAWAYEALSFGGFWAWDPVE
                     NASLVPWLVLAAGIHTLMAYKHTGYSLHATYVLFISTFLLVLYSSFLTKSGVLGDASV
                     HSFTDLGMSGQLVVIILAFLLPSVYLFIDRTFNKKLIPSKPEEEKLSSREFWLFIGSL
                     IFVFSAIHIIVLTSFPVINKIFGSKIAPPSDIKSHYNNVQIWMAAIVAIGSAITQFFK
                     YKHSDVKKVILSLLPAFVLSIALTIVVVIPYKLYRLDYVLMVFSAWFALLANVQYIFK
                     GLKGKMKIAGGSVSHIGFAIMIIGILISQGRQEVLSVNQFGIDYGQGFSEQEIAENVL
                     LYQNEPTKMGKYRVTYLGDSVTAQNIYFNVRYEELDKKGNVRKAFTLHPNILMNNKMG
                     NTPVPSTLKTITSDLYTHITSAPLKEDGTPADSLVENTYTISVGDTIPASRCMVVLES
                     INPDAHIHDFKMLPNDIAIGARLKFIAIDTTYNIEPIYLVREVVATSIPAKVDKHDVS
                     FSLIKILPEENKLQFLVQQKLNKFIIMKAIRFPFINVLWLGCLVMTVGVFMSMMRRKK
                     ENTTA"
     gene            complement(7556..7960)
                     /locus_tag="PLL59_04630"
     CDS             complement(7556..7960)
                     /locus_tag="PLL59_04630"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HMY23124.1"
                     /translation="MKRTHIIALIFVAVLVASLTVMLGKGFSRYEDFESAHARQGKEF
                     TVVGYLNKEKEIKYQPEINPNQFSMYVIDEQKVEKLVIVNKAKPRDIEKSEKVVVTGK
                     MNGDTFYANDILLKCPSKYKNREQQANDGRSI"
     gene            complement(8037..8576)
                     /locus_tag="PLL59_04635"
     CDS             complement(8037..8576)
                     /locus_tag="PLL59_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMY23125.1"
                     /translation="MQARIEPIEKPNGIFMRIGFFFMKRMFGKVITPAKTIYNRVPEM
                     LFVTKKFADVEAKITLEPILAKLIKHHVASINGCSFCIDINQRAAIDLKIYSEKFKHL
                     HQFETSPLFSEKEKSAFRYVEAVTRQKIVSDELFTNLKQNFNDTEIVEITYLCAMENF
                     YNCMNIPLGIESDNLCALI"
     gene            complement(8591..9178)
                     /gene="folE"
                     /locus_tag="PLL59_04640"
     CDS             complement(8591..9178)
                     /gene="folE"
                     /locus_tag="PLL59_04640"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HMY23126.1"
                     /translation="MENNELILESNPVIEKYYREILSHIGEDPNREGLLQTPNRVSKS
                     LQYMTHGYSIDPIEIVRSAVFNEDHNQMVIVKDIELYSMCEHHMLPFFGKAHVAYIPN
                     GKITGLSKIARVVDAYSRRLQVQERLTVQIRDCIQEALAPLGVAVVIEAKHMCMMMRG
                     VQKQNSVTTTSAFTGEFEKQATRSEFVKLISAKLS"
     gene            complement(9331..9669)
                     /locus_tag="PLL59_04645"
     CDS             complement(9331..9669)
                     /locus_tag="PLL59_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMY23127.1"
                     /translation="MSAIRNQVQLIGNLGKKPEIKSFGEDKKLATFSIATSDYYYDNK
                     GEKKEQTQWHNVVVFGKQAETVEKYLDKGMQVAVTGKITYRQYEDKDGIKRNVTEIIA
                     SDFLLLAKKN"
     gene            10083..11870
                     /locus_tag="PLL59_04650"
     CDS             10083..11870
                     /locus_tag="PLL59_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23128.1"
                     /translation="MNKLYKIIPIYLLYYSLTAQAANELYVKGQAATIAATTPTLYNN
                     GSLIHVNGDITIEDGLFVNTGSNFLELSGNWANNQASGNNKYESDGYERFFGTTDQTI
                     SGVMNGTTANNNQFYNLYISKTSTTGEYVSLLNNVHINAAGTLQFSDSYGIIRTQAST
                     TTTPDYTGNYTNELYIQNPSASAIVGHSAGAGATTKYIEGKLRRQANGTDTYFFPIGV
                     GKAGLDGMEAISINMNALSMPSGSTTGLLAYIQPAAIGLYSSDLITNGDVLFYDIGGY
                     DVATSNNNFSQCVSGPEGYDDVALIDQAITHEWIVTPNIAPTSVDYDITVVPGTILEN
                     QISYAVMGTPCNTLYPKAQYLARNGRIGGNTSFGPTYIFDVPNVLGLVQAPTLKTITG
                     QDGFSRFRLFGATDDNTSLPVELLSFTLTPINNQYFLLNWETASEFNNAGFYVQRSTD
                     RSNFENIAWIAGNGTTNQAQQYTYADRNVEQNITYYYRLQQIDINHAYKYSNILSGRL
                     IGTAFEVTDVHPNPSTVNPQVDVYAPNNGLLQTSIIDVLGREVGHQNFELNRSINALT
                     LNLSYLAKASYLLRFNYDNHLITKKFIKN"
     gene            11882..13018
                     /locus_tag="PLL59_04655"
     CDS             11882..13018
                     /locus_tag="PLL59_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23129.1"
                     /translation="MKKILLFTVASFFLGNIAMAQNNVGIGTNLPDPSAKLDVKATDK
                     GLLIPNVSLTAKNVAAPITSPAISLLVYNTNTSGAAPNNVYPGYYYWDGTIWVRLHSS
                     DNDWTKSSTVSTPAIKTDNQYITGNAGIGDFSTADPLKSLHIKHSANTGGLLLQNTTG
                     GAGSTTNIYFSTYNPATYPVASISAADVNSYSANLSFSTKLPNVSDGNGLTERMRIQN
                     TGEIFGKFRHITYHTFNQGGIYKRNSNDIFYIPGYGDAPDDINFGLTGAGSNDYKLQT
                     VVPYNGKLIKIIVRVGGDSGSNPDMAVMPVISKNGTYSRLDAGAGPTFSVESDQSGVY
                     IPTQNNSVTRGDRIAIGFAVNCANCWLENTNYYVALVWEYDIWD"
     gene            13340..13573
                     /locus_tag="PLL59_04660"
     CDS             13340..13573
                     /locus_tag="PLL59_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23130.1"
                     /translation="MTIHLHLIAEARDTYLMQNIVRDLKRHTSKQLLKAFKENAEESR
                     REWMLAIFKNAGVYNSNNIEYQFWRQDNKPIEI"
     gene            complement(14011..14748)
                     /locus_tag="PLL59_04665"
     CDS             complement(14011..14748)
                     /locus_tag="PLL59_04665"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMY23131.1"
                     /translation="MLLQRAIIFHPKKLPTHHEYKSKYAYTEHFYEFNISNENFIINA
                     VHLAAPNSKGFVFFLHGTTNHIQYHLPKTEFFIENNYDVFLMDYPTYGKSKGKLTEDL
                     LHKVVALTYAKAIEQATSTENIILLGRSLGTALASRLAIDVQPRSLILVSPYYSMPDL
                     FNSKLGLFPFKKLKFKLENFSAIPKVHCNTYIFHGNADKLIPIRLSQKLIPLLKSADH
                     YFEIDGANHFNVHQMETFKEKLKQILA"
     gene            complement(15012..15602)
                     /locus_tag="PLL59_04670"
     CDS             complement(15012..15602)
                     /locus_tag="PLL59_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMY23132.1"
                     /translation="MKKHLFGFLFMMFNVVVSCTANTNANNTILGTATTKTIYDFSMK
                     DIDGNDVSLSKYKGKIVVIVNTASQCGLVGQFAEIEDFYKKYKDKGVVVLGFPANNFL
                     GQEPLSNAEIKSFCAKNYGVTFPMFSKISVKGNDIDPLYKYLVNKDENGVLDAPVKWN
                     YQKFIIDRQGKVVTSISPRTTVNDVEFLQYIEDLLK"
     gene            15830..16561
                     /locus_tag="PLL59_04675"
     CDS             15830..16561
                     /locus_tag="PLL59_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMY23133.1"
                     /translation="MEYISSDKQFMESKNQENRRFHLLIADKDKLGIDGTVKALKTEN
                     YKITEVVKSKELIAFIKKEKPDLVLLEVDLQEADGIEICWDLRADENLSKLPIVFISD
                     RSDDFTQIAAFEAGADDFISKSSRSRLIGNKIKAILRRCYDKEEAPKEIKKFGNIEID
                     EEQVVIFKKGEPLKLSKKEFQLVLLLTSRPGKVFKRNYILLKVWGDDIIVGDRNIDTH
                     IKKIRKKLGKQHIETVRGIGYKFIP"
     gene            16705..17127
                     /locus_tag="PLL59_04680"
     CDS             16705..17127
                     /locus_tag="PLL59_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23134.1"
                     /translation="MNTRILIAATAGAILYFLLGWLIYDVLVADYYATHSVTQYYNGL
                     IKSNPSFIGIFFSCFAFTLVLAVIFGNMANITTIKDGALAGTTISLLVALYLNLNIWS
                     MFNMIGKTVVIADSLISAIVGGILGAVIAGILGYKRDA"
     gene            17270..19195
                     /gene="thrS"
                     /locus_tag="PLL59_04685"
     CDS             17270..19195
                     /gene="thrS"
                     /locus_tag="PLL59_04685"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMY23135.1"
                     /translation="MSIKITFPDGAVKEFNEGITALEIAGQLSNSLPKKVLGAEINGE
                     VQDATRPITSDATLKLLTWNDTDGKSTFWHSSAHLMAEAIESLFTGVKFGIGPPIENG
                     FYYDIDTGDKTLSLADLEKIEKKMLELAAKKNEYIRKSVAKADAIQYFTEKGDEYKLE
                     LLEGLEDGQITFYTQGNFTDLCRGPHIPNTGHIKAIKLMKLAGAYWRGDEKRKQLTRI
                     YGVTFPNQQELDDYLKMLEEAEKRDHRKLGQQLEIYTFDEEVGPGLPLWLPNGGFLIE
                     KIEELAKKTEEEAGYSRVRTPHIAKEALYLRSGHLPYYSESMFPPMELDNVKYYLKAM
                     NCPHHHKIFGAMPKSYKDLPIRLAEYGTCYRYEDSGSLFGLMRVRSLQMNDAHIYCTK
                     EQFEEEFRAVNAMYLKYFKIFGIEKYKMRLSKHEPSKLGQKYIDEPALWIETENMVRD
                     VLISSNIPFDEVPDEAAFYGPKIDIQIYSVIGREFTLATNQVDFAQGKRFNLKYVNKD
                     NQEEFPIIIHRAPLSTHERFIGFLLEHYAGNFPLWLSPKQISILPISDKFLAYAESVA
                     ATLKAKGFRVKLDERAEKIGKKIRDAEMMKIPYMTVIGEKEVENQQISVRKHGKGDLG
                     AFSIEDFAVLLQDELNN"
     gene            19274..19822
                     /gene="infC"
                     /locus_tag="PLL59_04690"
     CDS             19274..19822
                     /gene="infC"
                     /locus_tag="PLL59_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HMY23136.1"
                     /translation="MIPRRRPFIPKKKETEHRLNRDIRVPEVRLVGEEIEPGVYPIEE
                     ALRLAKEQEVDLVEISPKAVPPVCRLVDYNKFLYDKKKKEKEIKAKQKKTEVKEIRFT
                     PNTDDHDLDFKQKHAQKFLQEGSKVKIYVQFKGRAIQFKDRGELVLLQFADSLKEFGA
                     LEAMPNLEGKKMIAYIAPKKKG"
     gene            19826..20449
                     /locus_tag="PLL59_04695"
     CDS             19826..20449
                     /locus_tag="PLL59_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23137.1"
                     /translation="MKSYRIFLILTFFIFISYNTKAQFVPGAPTFIKTPYGNISIPTY
                     YHLPIAYWNVGDKSTAIITNDYYLIKLKNDSTVKVYGEIDLSDSIHSVLIKDKKKVVR
                     KIYPSETKYIAALGEKNVHVAGTPTDSCWIFKQLDDSVSLYSVVPRIGTEYSIFFSKP
                     GNSALIALNEANLLTLVGTDEKLVEMIKNKSYIKAVKKYNNKLKAKK"
     gene            complement(20545..21270)
                     /locus_tag="PLL59_04700"
     CDS             complement(20545..21270)
                     /locus_tag="PLL59_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23138.1"
                     /translation="MNDKVKLEKYNFVTLCYLNSYYNLGLDLQAESFIQNLSSTDRSE
                     IEIVSPFYKADTCIESSRLDNNYNNPYNLMFYSLYCNCPYFKLEIFNTNLQTLYNRYS
                     TDSITNKEAYQLTHLLFAIAYVKEKLNIKQLDNDIENKYATLITEKIINNAAVSIQDE
                     YKVEALMSMMFAGYYKQSFESLLLQLIKLQEKDGSWQLKDDNEYTEKSAKSAFQKFEY
                     NKYIQHNTIVGLWSLLAWEKLIQ"
     gene            complement(21401..>24254)
                     /locus_tag="PLL59_04705"
     CDS             complement(21401..>24254)
                     /locus_tag="PLL59_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23139.1"
                     /translation="GRHLQDRGDACGAGPGGGSGGGGGGGGGQGGTLGTGGWGSGSSF
                     ALYAYGAGTGTLINVTLTAGAFRAGGTLGTGGSGGNGGTGGCGGNGNNTSLTGANVLS
                     GGVDGTSGCPTGRTCGNCEVGAGGQGGRGGNGGKGGNGQPGANGLSIASNAQGSAVVN
                     SSNNGLIAGTTTVKYNKGCTNSIIPISKTTGANWLNLGSGGAIVNDVDATTSSFTMAS
                     NALDIYYTTVISGNDAKDISVVGPQYYSDVIFVDQARNVPQIKVVPHAICSGEKDTFK
                     LIASNNFTNASGTDADFEWSYRQYKINTTLIPGPFTWTTVTGAAPTPITFNNATSDSI
                     TYIVRFRTKDKCCGWSIYSYAEFVVYPAVTPHTTWTKNPNVSNICISNAASLTATAPT
                     GASPSSGGVGASCNTIYRYTTDGGVTWTAWSSTLPTISKVIGTTMLVNAYECKGTIDC
                     DTAFSTDTLKWIVDDTVQAIAAIVNIASCTNPGTATLAATTPAAGTGQWTYVSGTATT
                     TPAVPTSSTTLNVSNIPLGTNTTTYRWTVTNGACSDFTNLNIVMPADSLNSISMDTLG
                     CYTCSIQNGNTYYFHDYLGRIICKIQDLTGGPYSTGALGNTEVCLRIPTPNTSPVPLV
                     NTINPVAGPMPYLGRYWSINPDVPNVHAKVTFYFTGNEYNKLKTGANSTRYSFNTATD
                     LHVSKWPGGGGQFFNGPDNVKTQNNIPGGIMLPGGPSLGGNATWTNPTFAAYTTLYGG
                     DYEVSFIVDTFSTFYVHPIIYAYEVLPVELVSFTGQNIGERNKLEWVTSTEINTNKFV
                     VEKSINGTTWFYLGEVPAAGNSNATLNYQLFDETPVEGNNYYRLKIIDNDDSYKYSNV
                     ISINIQHNTQSNNVVAVYPNPVNEELNVLILSTEDRQINMNVYDILGQLISIQRVDIS
                     KGLNTVKLNTMSLSSATYILNFMLFDNVYNYKIVKN"
BASE COUNT         8138 a         4226 c         4024 g         7866 t
ORIGIN      
        1 tcttgcgttc ttgtgtaaga tatgctacag cataagcttg tggcccgaaa ggcacatctg
       61 taccttttac tacgattgta taatttcctt ccaatggatt atcaatagta atctgttcat
      121 tggtgttaat attgtcaatg ccgtgaagtg caataatttt tggactttga ggattgagct
      181 tccaaggcaa tacagtatca ccagttggtg ttactaattt taaatctaaa ttattgacaa
      241 tagatttttg tgcaagcgga ctgccttcta tatctgtcca cgacaaggta acttttaatt
      301 gatgcgtatt tgctggtgaa gaaaacgagt gtgtttgtat tgattggtga tttacagtat
      361 tctcaaaata ctgttgatta gaaatagatt ttactgctct gaatgcatct ggttttccaa
      421 aaccatacat gtaatcagga cctgcatttc ctaaatctcg tgctgtatta aacaagattg
      481 cttttaacaa tgccgcattc ggtaattgag caaactgttg tttatattgt tgtgtcagca
      541 atgctgcaat acctgcaact tgtggtgctg catacgaaga accatatact gctgaaatgc
      601 catcattagg aactaaggca ctcacggcaa atccttttgc tactaattca ggttttaatc
      661 gaccatcttc tgtagggcca cgaccactgt tttctaccaa tttttcattg ccaaataacc
      721 aacctactgt aatggcattt ttacaacctt gaaaaccgat atcgacagag ctaaatgtat
      781 cagtagcaat ggcacaatta gcagctgcag tattaccaac agcaactata ggtaataatg
      841 ttggttgctc atgtacgatt ttatctaaat tggctgcttc aggaatatac aatccggatt
      901 ggaaacaatt tgtagaaacg aaattataag agtggttgga aatatctaca ttataagtcg
      961 atttgcctgt ttccaattct tgcaaaatat cacctgcaac gtcccaatac actatatttg
     1021 attgaggtgc aatacctttt atagaaggaa ataaattccc tgcactagca atacagccac
     1081 ctacttcggt agggtgatac atgggaataa atgagctata tgttttatca acaacaaaat
     1141 ttttagattt tggcaaatcg atatggtcgc caaatgcacc ttcgtcccaa acaccgactg
     1201 taacaccatt gcctttcaaa ttaaaaccat atggttgcac ttcttgcact tgattggtac
     1261 ctgtcatcaa caagctttca tacattaatg gatttcgagt gctataatat tttgaaataa
     1321 aaactacaaa aggatatcta gataaatcct gtatttgttg attattcaaa gtagcattaa
     1381 aactgtgata tttcgaatta aaattattga tgtgaatacc atgttgtgaa agaatattag
     1441 caagccaatt ttcatcaatc gttgaagtca gcaatacact tacctcttgt gctgtattgg
     1501 ttgacaataa actattatca attttacttt cgcttgacaa atacgatata gaagaatatg
     1561 gcagtgcatt tattttattt gagacattac tattgataga tgttagataa tatccattgc
     1621 ctaagtaagc ttctatagaa aaaccctgct gaactaaata atcagcatta ttatttattg
     1681 tttgtatagt aagataatct acattattga atctaaaatt ttgagtagta gttcgcgcag
     1741 aagtagattg taaaattgca gggtcaattt ttacgagttg taaattattt tttgaaaaaa
     1801 cagatatact gaatgcaata agaaatataa acaaagcgta tgtctttgtc ataagcaaaa
     1861 agatttagta taaatataag attttagctg gaatacggca aaataaaact tttcacaata
     1921 gtcgaaattt atttcatttc tacaatggta attccgtcgc ctccaaattg ttcagcttct
     1981 acacggaaag tcttaacatg aggatatttt tttaaataga aatgaactgc ttgtttaagt
     2041 gcgccagtgc ctctgccgtg tattatttta agttgatgaa tgccatacat tacgaccctg
     2101 tccatataat tatctaaagc taaaatggct tcatctttaa gcattcctct aatgtctaaa
     2161 ctattatcaa attcactttt ttctactaaa attttggagg agaatttatt cctgctaatc
     2221 cttacttctt tcgatgattc ttgtattaat tgtagttgat gcagttttac tgttgattgc
     2281 atactgccga atgcaactac agctgtatta ttttttaatg aaagtattac gcctatttcg
     2341 gtgccttcta ataatttaac ttttgcgcct acaattaatt ccttgttgtt gatatttgat
     2401 ttttcaactg gcgcatcatc taacttacgt tcgattgatt ctctgttatt atctataaat
     2461 tttctgaccg taataaactt ttctttacta tctttttgtt ctttccattc gcgcatctca
     2521 ttttccagat ttctgctttc cgcattaaac ctttctaaca agcgtgcttc atattgtttt
     2581 agcaatcgtt ttttttcttt ctctaaatct ttattcaact gcgtataact attggtcaag
     2641 tcctgcaatt gtgtttcttt ttgttgtaca tttttgcgca atccttttat gaaatgcttt
     2701 tcagtttgaa tatctgtcaa cacatcatct aaagcttttt tgttatcgcc aattttcaag
     2761 tttgcctgtt gtataatttt tgatggcaat ccacttttac gtgcaatttc gaaagtgaat
     2821 gagctaccag gttgccctaa ttgcaattga tataatggtt ttaattgctg tgtgtcaaat
     2881 gccattgcgc cgctttgcat gccagcagta tttgctgcaa aaacttttaa attattgaaa
     2941 tgagtggtaa cgaccccaaa cacttgtttt tgatgcagac tctctaatat agcttctgcc
     3001 attggaccac ctaatgctgg atcggtgccc atgcccattt catcaattaa cactaatgta
     3061 gagcacgatg cgtggcttaa aaaatagttc atatttgata agtgtgaact atatgtactc
     3121 aaatcatttt ctatggattg ctcatcacca atatcaacaa ataaattttc aaatatagag
     3181 acaatagaat tttcttttgc tggaatcaac ataccgaatt gtaacatcaa ctgtattaaa
     3241 ccaatcgatt ttaatacaat agattttcca cctgcatttg gaccagagat aacgacaatc
     3301 ctattttttg catctaaaaa aatcgcattg tcaataattg gtttattttg tttttggtga
     3361 tggtaaaaca aaacaggatg cacaaattcg cgaagatgaa tgattggctc atttgacaat
     3421 tgcggcatat tgcaattgaa gtttatcgca aaataagctt tagcgcgaat gacatctaag
     3481 tcagccatta agtgctgcaa tttgtcgagt gttgttttat gtggccgaat gtaattggtg
     3541 agcgatatca atattttcaa caattctcgt ttctccagca agtacagttc cgtcagttca
     3601 ttattaagga aaagcgtttc atttggctca ataaatgtaa tagaaccgtt gtcgctctca
     3661 tcggtaatta atcctcttat actgcgtttg tattctgctt ttacagaaag tactcttctg
     3721 ccatcacgca ccgtttcttc tgtttctgct aaaaaatttt gttgcttata ctgagacaac
     3781 actcttctaa aaactgcatt tatatcttgg attttatgtt gaatatcctt gcgaattttt
     3841 gacaattctg gtgttgcatt atctctgata atttctttgt caacgtcaat cactttagtg
     3901 atagaagcaa ttataccaag ttcaatttga tattcggcca ctttctccga caagaaagga
     3961 tacaatagct gacgtgcttt tggcgtaaaa aacctaaaca catctgcggc ggcgcttaat
     4021 gaaaagtaaa ctgcaattaa ttcttttact tctaaaaaat aattttcaat ttgtagtttc
     4081 tctaaaaatg gtaatgtatt aaatccatat tgaggaagct gcgtatcatt ttcaataatc
     4141 tcttttaatt cctttacaaa gtgtaactgt ttgtttactt catctgtgtt agtaataaaa
     4201 gataaatctt gtaataatgc ttttcccaag ctaccactac atagattttc taattctttt
     4261 agaataaaag gaaagtctaa tttttgtaag ctattttttg gatatacatg cattaagaat
     4321 acattttcaa aatcgaattt gtcaataatt gataaatttg aaacagttgt gagtcttcct
     4381 cctttagcat agtaagatgc ttgtctataa tttcgacatc atttcttctt gcaggaccag
     4441 tttgtgcatc ttttggtaaa atggaatcaa tttttgtaat tgtttcatgt atcaaaggca
     4501 taagcaactg ataatccaat tggtgcttct cgcaataatt ttgtgcaatg gcaaataaat
     4561 gattggaaaa attatttaca aaaactgcag caatatgtag atgtttacgc tgttcgtctg
     4621 tgtattcata aactttatca ctcaatagtt ttgccaatga catgagttca tttttaaaca
     4681 catcattatt agaagtaatt aaaaatggaa ctgcttcaat atcgattttt tttgtttttg
     4741 aaaaagattg taatggataa aaaatgcctg tatttgtggc attttcaaac acatctaagc
     4801 cgacggagcc agaagtgtgt accattttca tgtctggttt tttctttaaa atatattgcg
     4861 ataaagtttt gatgacatca tcttttacag caaaaatcaa taagtcaact gataaatcta
     4921 attcttctaa atcacatgta taaatcgaat tagattgcca agctaatgtt tcagaattat
     4981 cacaagattg gctataaatt tgctttactg taattccttt tttagcaaat gctttagcca
     5041 aatgtgtagc tacatttccg ctgccaataa atgaaatatt ttgtatcgca tcttgcataa
     5101 aattatgctg ttgtattttc tttttttctt ctcatcatac tcataaaaac accaacagtc
     5161 ataaccaaac agcccaacca aagcacatta atgaatggga aacgaatagc tttcatgata
     5221 ataaatttat tgagtttttg ttgtacaaga aattgtaatt tattttcctc tggtaaaatt
     5281 tttatcagtg aaaaactgac atcatgttta tccactttag caggaatact tgttgctacc
     5341 acttcacgca ctaaatagat aggttcaata ttatatgttg tatctatagc aataaacttt
     5401 aagcgagctc ctatcgcaat gtcgttaggc aacattttaa aatcgtgaat atgcgcgtca
     5461 ggattgatgc tttctaagac caccatacaa cgagaagccg gaatagtatc gccaacagaa
     5521 atcgtgtacg tattttctac taaggaatct gctggtgttc catcttcttt caatggtgct
     5581 gatgtaatat gtgtatataa atcagaagta attgtcttca gcgttgaagg cactggcgta
     5641 ttgcccattt tattattcat caaaatattt ggatgcaagg taaatgcttt tcgcacattt
     5701 ccttttttat ccaattcttc atagcgtaca ttgaaataaa tattttgtgc tgtaacagaa
     5761 tcaccgagat aagtaactct atattttccc atttttgttg gctcattttg atacaataaa
     5821 acattctcag caatctcttg ctcagaaaat ccttgaccat aatcgatacc aaactgattt
     5881 acggacagca cttcttggcg accttgtgaa attaaaatgc cgattatcat aatggcaaag
     5941 ccaatatgcg aaacagagcc acctgcaatt ttcattttac ctttcaatcc tttgaagata
     6001 tactgcacat ttgcgagcaa tgcaaaccaa gcactaaaca ccattaacac ataatcaagg
     6061 cggtacagtt tatatggaat tactactact attgtcaatg ctattgacag cacaaaagca
     6121 ggcaataaag agagtattac tttctttaca tctgagtgtt tgtatttaaa aaactgcgtt
     6181 atagcagagc caattgcgac aatggctgcc atccaaattt gtacattatt ataatggctt
     6241 tttatatcag aaggtggcgc aatctttgaa ccgaatattt tattgataac tggaaaagat
     6301 gtcaatacaa taatgtgtat tgcagaaaaa acaaaaatta aagaaccaat aaacaaccaa
     6361 aactcacgtg atgataattt ttcttcttcc ggtttagatg gaattaattt cttgttaaat
     6421 gttctatcta taaacaaata gacagatggc aataaaaatg ctaagataat gaccaccaac
     6481 tgaccgctca tacctaagtc ggtgaaagaa tgcacagaag catcgcctaa aacaccactt
     6541 tttgtgagga aagaagagta caatactaat aaaaacgtac tgataaataa tacatacgtc
     6601 gcatgtagcg agtagccagt atgcttatat gccattaatg tgtgaatgcc ggctgctaat
     6661 actaaccaag gtactaatga tgcattttct acaggatccc atgcccaaaa accaccaaaa
     6721 cttaacgctt catacgccca agcgccacct aacaaaatac ctgcacccaa tatcatcaaa
     6781 gtaaataagc tccaagtcaa ggcaggtttt acccaatcag taaaatttcg aacccaaagt
     6841 gatgcaatgg cataagccgc tggaatggtg caacctgcaa atcctaaaaa tagtgttggt
     6901 ggatgaatga ccatccaata attttgtaac gatgggttca atcccgttcc atcttccgct
     6961 acccattcag gattcatttt aaatactgga atatccattg tgtcgcgtac tagtgcgaat
     7021 ggactggaac caaatttata atgaaaaaga ataatgccta atagcatcga acctaataat
     7081 acttgcgcta atgcaactat agacaacaca ggactttccc aattttttgc agtaaaaacc
     7141 aatacacaac ctaacactgc atgccaaaac atccataata aggtgctacc ttcttgacca
     7201 ctccacatag atgaaaactg gtaataccat ggtaaagatt ttgaagtatg ctcccaaaca
     7261 tatttatact cgaaataatg accgtgtatt attgtcaaaa gactaatgat gatgcctaag
     7321 acagcgactg catgaatcac aaaagctttt ctaccaaatt gtttccaaga caatgatttt
     7381 gataaatcat ttctataagt agttgcaaaa aaataagcaa ggcatgctgc taatgctgcc
     7441 acaaaagata agattaccaa aaaatgtccg acttttccta tcaacaaatt ttgaccaatt
     7501 gcataaatat tcatattaat gataataaaa gtgtataaaa atacacgatg agaaattaga
     7561 tacttctgcc gtcattggct tgctgttctc tatttttata tttagatggg cattttaata
     7621 atatatcatt ggcataaaaa gtatcaccat tcatttttcc ggttactacg actttctctg
     7681 atttttcgat atcgcgaggt tttgccttgt tgacgattac caatttttcg accttttgct
     7741 catcaattac atacattgaa aattggtttg gattgatttc aggttgatat tttatttctt
     7801 tttctttatt caaataacca acaacggtga actctttacc ttgtcttgca tgtgctgatt
     7861 cgaaatcttc gtagcgacta aaacctttgc cgagcattac ggtgagtgat gctaccaaaa
     7921 cggctacaaa aatcaatgct atgatgtgcg ttcttttcat ggaaacaaat ttcaagtttg
     7981 aacatcaaaa gtacggctct ttacctaaaa aaaaggaaat aaaaggttat ttaattttaa
     8041 atcaatgcac aaagattatc actttcgatg cctaatggaa tattcataca attataaaaa
     8101 ttttccatag cacataagta agtaatttct acaatttctg tgtcgttgaa attttgtttt
     8161 agatttgtaa aaagttcatc tgaaactatt ttttgtcttg taacagcttc cacatatctg
     8221 aatgcggatt tttctttttc tgaaaacaat ggagatgttt caaattgatg caaatgctta
     8281 aatttttctg aatatatttt taaatctatt gctgctcttt gattgatatc aatacaaaag
     8341 ctacaaccat taatgctagc aacgtgatgt tttatcaatt tcgccaaaat gggttctaaa
     8401 gtaattttcg cttctacatc tgcaaatttt ttcgttacaa ataacatttc tggcactcta
     8461 ttgtagattg tttttgctgg tgttattact ttgccaaaca tgcgtttcat gaagaagaaa
     8521 ccaatacgca tgaatatgcc atttggtttt tcaattggtt caattctagc ttgcatttgc
     8581 aaaagattaa ctacgataat tttgcactaa ttaatttcac gaattctgat cgtgtagctt
     8641 gtttttcgaa ctcgccagtg aaggcagaag ttgtcgtaac agaattttgt ttttgtacac
     8701 cgcgcatcat catacacatg tgtttagctt caataacaac cgctacacct aatggtgcta
     8761 atgcttcttg aatacaatcg cgtatttgca ctgtcaatct ttcttgcact tgcaaacgtc
     8821 ttgaatatgc atcaactaca cgtgcaattt tactcaaacc tgttatttta ccattaggaa
     8881 tataagcaac atgggctttg ccgaaaaatg gcaacatgtg gtgttcacac atcgagtaaa
     8941 gttcaatatc ttttacaatc accatttgat tgtggtcttc attgaatact gctgaacgta
     9001 ctatttctat tggatctata gaatagccat gtgtcatata ctgcaatgat tttgaaacac
     9061 gattgggtgt ttgtaataag ccttcacgat tcggatcttc acctatatgt gacaagattt
     9121 ctctataata tttttcaatt acaggatttg actccagtat taattcattg ttttccattt
     9181 catccatgtt ataaaagtac aaaaagtata aaacatgaaa aaatgatttt tgtttagaag
     9241 aaaataagga actgaataca gaatagaaac atacaaaaaa aggctgtctt aaaaagacaa
     9301 cctcacgaac taaacaagta caccgaggaa ctaattcttt tttgctaata gtaaaaagtc
     9361 gctagcaata atctctgtaa catttctttt tataccgtct ttatcttcat attgacgata
     9421 agtaatttta cctgtgacag cgacttgcat acctttatca aggtattttt ctacagtttc
     9481 tgcttgcttg ccaaaaacaa ctacattgtg ccattgcgtt tgctcttttt tctcgccttt
     9541 attatcataa taataatctg aggtagcgat actaaatgtg gccaattttt tgtcttcgcc
     9601 aaaggattta atttctggtt ttttgcctaa gttgccaatt agctgaactt gatttctgat
     9661 tgctgacatg tttttaattt taattacgtt aaaaaatcaa ttttagttat tcagcattgt
     9721 cgtgtattat cgcgaataag tatttccact tctttgctgt tttcacctat tgagagacaa
     9781 gagctattaa aattccataa ataattttga aaatattttt cacaaacaac ataatacatt
     9841 gagaataaat catttgtaat taatattttt tttatttgct tattgcttgt acctaccgat
     9901 cggtgtcctc agcgaacgat acgaaaattt gttttatgag attcccactt tcgggggaat
     9961 gactgttcgt gagaatgtcg ttttgacaaa agtcaaaaaa aacttaacac tagcataata
    10021 tacaagaaaa aacttgacaa ttgtcaagat gaacaataca tttaaattac aaaaaaacaa
    10081 ctatgaataa attatacaaa atcattccaa tttatcttct ctattattca ctcacagctc
    10141 aagcggcgaa tgaattgtat gtaaaaggac aagcggctac tatagctgct acaacgccaa
    10201 ccttatacaa taatggttca ttaatacatg taaatggcga catcactatt gaagatggtt
    10261 tgtttgtcaa cacaggcagt aattttttag aactatctgg gaattgggca aataatcaag
    10321 ctagtggtaa taacaaatac gaaagtgatg gctacgaaag attttttggt accactgacc
    10381 aaactattag tggcgtaatg aacggtacaa ctgctaacaa caatcagttt tataatttat
    10441 atatcagtaa aacaagtact actggcgaat atgtaagttt attaaacaat gtacacatca
    10501 atgctgcggg cacacttcaa ttttcagatt cctatggtat catacgcacg caggcaagca
    10561 caacgacaac accagactat actggaaatt atacaaatga actttatata caaaatccat
    10621 cagctagtgc aatagttggt catagtgctg gagctggcgc tacaacaaaa tatattgaag
    10681 gtaaattaag aagacaggct aatggcactg acacctattt ctttccgatt ggcgttggaa
    10741 aagctggttt agatggtatg gaagcgatat caataaacat gaatgcatta agcatgccat
    10801 ctggttctac tactggatta ttagcctata ttcaacctgc tgcaattgga ttatatagta
    10861 gcgatttaat tacgaatggc gatgtattat tttatgatat tggtggatat gatgtagcaa
    10921 ccagtaataa taatttttct caatgcgtta gcggaccaga aggatatgac gacgttgctt
    10981 taattgacca agccattaca catgaatgga ttgttacacc caatatagca cctacttctg
    11041 tcgattatga tattacggta gtaccaggaa caatccttga aaatcagatt agctatgccg
    11101 taatgggaac gccttgtaat accttgtatc cgaaagcaca gtacttagct agaaatggaa
    11161 gaattggtgg caacacttca tttgggccga cctatatttt tgatgttcca aatgtgcttg
    11221 gcttagtgca agcaccaaca ttaaaaacga ttacagggca agatggtttt tcaagattta
    11281 gattattcgg agcaacagac gacaatactt ctcttccggt agaacttttg agctttacac
    11341 tcacaccaat caacaatcaa tattttttat tgaactggga aactgcttct gaatttaata
    11401 atgctggttt ttatgtgcag agaagcaccg acagaagcaa cttcgaaaac attgcttgga
    11461 tagctggaaa cggcaccact aaccaagcgc aacaatacac gtatgccgat agaaatgtag
    11521 aacagaacat cacctattac tatcgtttgc aacaaattga tatcaatcat gcatacaagt
    11581 attcaaatat actttctggc agattgattg gaacagcatt tgaagtcact gatgtgcatc
    11641 caaatccttc gacagtaaat ccacaagttg atgtgtatgc tcctaataat ggacttttac
    11701 agacatctat catcgatgtt ttaggcagag aagtaggaca tcaaaatttt gaactaaaca
    11761 gaagcattaa tgctttaacg cttaatttat cttatttagc taaagcatct tatctgctac
    11821 gatttaacta cgacaatcat ctaattacta aaaaatttat taaaaattaa aaacacaaaa
    11881 catgaaaaaa attttactat ttactgttgc ttcctttttc ttaggaaaca tagcgatggc
    11941 acaaaacaat gtaggcatag gcactaacct accggatcca tcagcaaaat tagatgtaaa
    12001 agcaacagac aaaggattat tgataccgaa tgtatcttta acagcaaaaa acgttgcagc
    12061 gccaataact agcccagcca tatcactcct agtatataac accaatactt caggagcagc
    12121 accaaataat gtatatcctg gatattatta ttgggatggt acaatatggg tacgcttaca
    12181 ttcttctgat aacgactgga caaaatcttc tactgtttca acacctgcaa taaaaacaga
    12241 taaccaatac attactggta atgcaggtat aggtgatttt tcgactgctg atcctttgaa
    12301 atctttacat ataaaacatt ctgctaatac gggtggatta ctattgcaaa atactacagg
    12361 aggtgcagga agtactacaa acatttactt ttcaacgtat aaccctgcga cctatccggt
    12421 tgcaagtatc agtgcagcag atgttaattc atacagtgct aatttatctt tttctacaaa
    12481 actacctaat gtttccgatg ggaatggttt aacggaacga atgagaatac aaaataccgg
    12541 tgaaatattc ggaaaattta gacacattac ttatcatact tttaaccaag gtggtattta
    12601 taagcggaat tcaaatgata ttttttacat accgggctat ggagacgcac ccgatgatat
    12661 aaactttggt ctaacaggag cagggtcaaa cgactataaa cttcaaacag ttgtaccata
    12721 taatggtaaa ttgattaaaa ttattgtacg cgtaggtggt gatagtggta gtaatcctga
    12781 catggcagtt atgcctgtta tatcaaaaaa tggaacgtat agtagattag atgcaggagc
    12841 aggaccaact ttcagtgtgg aaagtgacca atcaggggtt tatattccaa cacaaaacaa
    12901 cagtgttaca agaggcgaca gaatcgcaat cggttttgct gtcaactgcg ctaattgttg
    12961 gttagaaaat acaaactatt atgttgcatt agtttgggaa tacgatatat gggattaata
    13021 attgaaatat tactggaatt cccaatgctg tcgcaagttt gtagtgtagg caaaaagcat
    13081 tgctaacttg tgacagtaaa taaagcaagt ttgtaacttg cgatgctata aatatttcta
    13141 tggtataata gaaatatgta actttattac atgagtcgag gcaataaaat ccataatcca
    13201 aaaggtattt attttataag ttttgcaaca gtaggttaaa tagatgtttt tactaaatta
    13261 tttataaaga tattttagta gaaagtataa aatattgcca aaaggaaaaa ggcttaaatt
    13321 tgtatacatg ggtaatttta tgacaatcca tttacatctg atagcagaag caagagacac
    13381 ttatttaatg caaaacattg ttagagattt gaaacgacat acaagtaaac agttgctaaa
    13441 agcctttaaa gaaaatgcag aagaatcgag aagagaatgg atgttggcaa tatttaagaa
    13501 tgcaggcgtg tataacagca ataatataga atatcaattt tggagacaag ataataaacc
    13561 gatagaaata tagacgagtg caataataga acaaaagtta acttatttgc atgctaatcc
    13621 tgtaggggca ggatatgttg ataaggaaga agaatatctt tacagtagtg cgagcgatta
    13681 tatggtactg gaaaaggatt gatagatgat tattgttata gaaattatta agttacaaac
    13741 ttaatttatt tgagttcaca agttacgtca gccaaggctg tggctttgtt ttgctaaact
    13801 tgcgagagcg aagggtgctt gagcatacat aagatatatt ttaagtatta tataaacaac
    13861 gcacaagtac gcatcagcac aaatcaaatc tgcatacttg cgcaagttgg gagagcgaaa
    13921 aaacgattaa tatttgccaa gcgtttttat ttgaattcat agtaagtaaa gcaaaatagc
    13981 tgttgtctat ttaaaaacga aaaaaaatat ttatgctaat atttgtttta gcttttcttt
    14041 aaaagtttcc atttgatgta cattaaaatg attggcgcca tctatctcaa aataatgatc
    14101 tgctgatttt aataatggta tcaacttttg cgataaacgt ataggaataa gcttatctgc
    14161 attaccatga aaaatataag tattgcaatg tacttttggt atagcggaaa aattttctaa
    14221 tttaaatttt aattttttaa aaggaaacag tcctaattta ctattaaaca aatcaggcat
    14281 actgtaataa ggtgatacta aaataagtga acgcggttgc acatcaatcg ctaatctgct
    14341 ggccaaagca gtgcccaaag aacgaccaag caatataata ttctctgtag aagtggcttg
    14401 ttctattgct ttcgcatacg tcagcgctac tactttatgt aataaatctt ccgtaagttt
    14461 tcctttactt ttgccataag ttggatagtc catcaagaaa acatcataat tgttttcgat
    14521 aaaaaattct gtttttggca aatggtattg tatatgattg gtggtaccat gcaaaaaaaa
    14581 cacgaagcct ttgctattcg gtgctgctaa gtgaactgca ttaataataa aattttcatt
    14641 cgaaatatta aactcataaa aatgctctgt ataggcatac ttacttttat actcatgatg
    14701 cgttggcagt tttttaggat gaaatatgat agctctttgt agtagcatat taattaataa
    14761 acaagtagta caatatacga aatattttac aagatacgct tttgtacgtc tttccctatt
    14821 gcttggtgcc tcaccgacct atctatatat tattataaaa aattaaaatc acattagaat
    14881 taaaatctat catgttttta ggtgtcaaaa aaaatcaagt tcatcataaa cttattatat
    14941 tttacaaata aaaaaaggct tggataaatc caagcccgag atttgtgttt cagactctgt
    15001 ctacattttt gttatttcaa caagtcttca atatattgca aaaattcaac atcatttaca
    15061 gtagtgcgcg gactaatcga cgtgactact ttgccttgtc tatcgataat gaatttttga
    15121 taattccatt ttacgggtgc gtcaagcacg ccattttcgt ctttattgac caaatattta
    15181 tacaatgggt ctatgtcatt tcctttcact gatattttag aaaacatagg aaaagtaacg
    15241 ccataatttt ttgcacaaaa cgatttaatt tcggcattgc ttagtggttc ttgtcctaag
    15301 aaattattgg cgggaaatcc taaaaccaca acacctttat ctttatattt tttataaaaa
    15361 tcttctattt cagcaaactg accaaccaag ccacattgac tggcagtatt cacaatcact
    15421 acaattttac ctttgtattt tgaaagtgaa acatcattac catcaatgtc tttcatcgaa
    15481 aaatcatata ttgttttagt agtagcagtg ccaagtattg tattattggc attcgtattg
    15541 gctgtgcagc ttactactac attaaacatc ataaataaaa aaccaaacaa gtgttttttc
    15601 atgcatttca ttttacgtta acaatattac cattttatac tatcaatggc aaattatcat
    15661 tttatatttc aatattgcaa atatcattcc aagtttcaaa atcttaaaaa tactaaaaac
    15721 actaaaaaca cataaacacc tcaaaaacgt caatttagca tcaataatta ttgtaattta
    15781 tgtgaatatt gtaagtagtt ttcaaaaatc agattaactt tagcagaata tggaatacat
    15841 tagcagcgac aaacaattta tggaatcaaa aaatcaagaa aacagaagat ttcatctact
    15901 tatcgcagac aaagacaagc ttggaatcga tggtaccgta aaggcattaa aaaccgaaaa
    15961 ttataagata acagaagtcg ttaaaagcaa agaactaata gcatttatca aaaaagaaaa
    16021 accagatttg gtgcttttag aagtggattt gcaagaggca gatggtattg aaatatgctg
    16081 ggatttacgt gctgacgaaa atctttcaaa attaccaatt gtttttattt cagatcgttc
    16141 agatgacttt acacaaatag cagctttcga agcaggtgct gatgatttta tcagcaaatc
    16201 ttcgcgttca cgtttaatag gcaataaaat aaaagctata ttacgtcgat gctatgacaa
    16261 ggaggaagcg ccgaaagaaa tcaaaaaatt tggtaatatc gaaatagatg aagaacaggt
    16321 tgtcatcttt aaaaaaggtg agccactaaa attatcaaag aaagagtttc agttagtttt
    16381 actactcacc tctcgccccg gaaaagtatt taaacgaaat tatattttac taaaagtttg
    16441 gggcgacgat atcattgttg gtgacagaaa tatcgataca catatcaaaa aaataagaaa
    16501 aaaattaggc aagcaacaca ttgaaactgt aagaggcata ggttataaat ttataccgta
    16561 gttggctatg catactaatt cataccgtta tacaacatgt atgaattcag cagtttacag
    16621 tatttctaca gttcaatgtt gataaaacaa attgcttctt atagaaagaa gtgttaattt
    16681 gagtaacaca aaaatcaaat acgtatgaat actcgaattt taatcgccgc cacagctggc
    16741 gcaattcttt actttcttct aggttggttg atttatgatg tgttggtagc cgactattac
    16801 gctactcatt cggtcacaca atactacaac ggcttgataa aatctaatcc aagttttatc
    16861 ggcatctttt tttcttgttt cgcattcacc ttagtattgg cagtaatttt tggcaatatg
    16921 gccaacatca ccacaataaa agatggtgct cttgctggca cgaccatttc gctcttagta
    16981 gcgttatacc tcaacttaaa tatttggtct atgtttaaca tgattggaaa aacggtcgtc
    17041 atcgctgatt cattaatatc tgctatagta ggtggaattt tgggcgctgt catcgctgga
    17101 atattaggtt ataaaagaga cgcataatta cgtctatctg tcgctgtcct caccgaccga
    17161 aactttgaca caatcttttt attattctat ttcaaaacaa gcccactttt ctataaacaa
    17221 taacttaaat tggaaaactg tattttatgc ttatttttgc aagtataata tgagtattaa
    17281 aattacattt ccagatggtg cagtaaaaga attcaatgaa ggtattactg ccttagaaat
    17341 agcaggacaa ttgagcaaca gcttgcctaa aaaagtttta ggtgctgaaa ttaatggtga
    17401 agtgcaagat gctacgcgac ctattacaag tgatgcaacg cttaaattgt tgacttggaa
    17461 cgacacagac ggaaaatcca ccttttggca ttcttcagct cacttaatgg cggaagcaat
    17521 tgaatcacta tttacaggtg taaaatttgg cattggccca ccgatagaaa atggttttta
    17581 ttacgatatc gatactggcg acaaaacatt aagcttagct gatttagaaa agattgagaa
    17641 aaaaatgctg gaattggctg ctaaaaaaaa cgaatacatt cgcaaaagtg tagccaaggc
    17701 tgatgccatt caatatttta ctgaaaaagg tgatgaatac aaactcgagt tgctagaagg
    17761 cttggaagat ggacaaatta ctttttatac gcaaggcaac ttcaccgatt tatgtcgcgg
    17821 accacatata cctaatacag gtcatataaa agcgataaag ctaatgaaac tggctggcgc
    17881 ctactggcgt ggcgacgaaa aacgcaagca attgactcgt atttatggcg ttacctttcc
    17941 taatcaacaa gagttagatg attatctaaa aatgttggaa gaagcagaaa aacgcgacca
    18001 tagaaaatta ggtcagcagt tagaaatata tacttttgat gaagaagttg gtccaggctt
    18061 gccgctatgg ttgccaaatg gcggattttt aattgaaaaa attgaagagc tggccaaaaa
    18121 aacggaagaa gaagctggct actcgagagt gcgcacgccg cacattgcca aagaagcttt
    18181 gtatttgaga agtggacatc ttccctatta ttctgaaagc atgtttccac caatggaatt
    18241 ggataatgta aaatattatc taaaggcgat gaactgcccg caccatcaca aaatatttgg
    18301 cgccatgcct aaatcgtata aagacttgcc gatacgttta gcagagtatg gcacttgtta
    18361 tagatatgaa gactcaggta gtttatttgg cttgatgcgt gttcgttctt tgcaaatgaa
    18421 cgacgcacat atctattgta ccaaagagca gtttgaagaa gaatttagag cagtaaatgc
    18481 tatgtatttg aaatatttta aaatattcgg catagagaaa tacaaaatgc gcttaagcaa
    18541 gcatgagcct tcaaaattag gtcaaaaata tatcgacgaa ccagcgcttt ggatagaaac
    18601 ggaaaatatg gtgcgcgatg tattgataag ttcaaatatt ccatttgatg aagtgcctga
    18661 tgaggctgca ttttatggtc caaaaatcga cattcaaatt tattctgtca taggaagaga
    18721 atttacacta gcaaccaatc aagtggactt tgcacaaggc aaacgtttta acttaaagta
    18781 cgtaaacaaa gacaatcaag aagaatttcc aattatcata cacagagcac cattgagcac
    18841 acatgagcgt tttattggtt tcttgttaga acactatgct ggcaatttcc cattatggtt
    18901 gtcacctaag caaatttcaa tattaccaat cagcgataag tttttggcgt atgcggaaag
    18961 cgttgcagca actttaaaag caaaaggatt tagagtaaaa ttagatgagc gagcagaaaa
    19021 aataggcaag aaaattagag atgctgagat gatgaaaata ccttacatga ccgtaattgg
    19081 tgagaaggaa gtagaaaatc agcaaatttc tgtacgaaaa cacggaaaag gtgacttagg
    19141 tgcttttagt attgaagact ttgccgtatt attgcaagac gaattaaata attaaacaat
    19201 aaaacaatgg attaatgagt atttctaaca aaataattca taattcataa ttcataattc
    19261 ataatttaaa aatttgatac ctagaagacg accatttatt ccgaagaaaa aagaaacgga
    19321 acacagatta aacagagata tacgcgtacc agaagtgcga ttagttggtg aagaaattga
    19381 gccaggcgtt tatccgattg aagaagcact tcgcttagca aaagaacaag aagttgattt
    19441 agtagaaatt tctcctaaag cagtgccgcc cgtttgccga ttggtggact acaataaatt
    19501 tttgtacgac aagaagaaga aagaaaaaga aataaaggcg aaacagaaaa aaacggaagt
    19561 taaagaaatt cgatttacgc caaacacgga cgaccacgat ttagacttca aacagaaaca
    19621 tgcacaaaag tttttgcaag aaggctcaaa agtgaaaatt tatgttcagt tcaaaggacg
    19681 tgctatacag tttaaagaca gaggcgaatt agtattactg caatttgctg attcattaaa
    19741 agaatttggt gcgctggaag cgatgccaaa tttagaaggc aaaaaaatga ttgcttatat
    19801 tgctcctaag aaaaaaggat aattaatgaa atcttatcgt atttttctta tactcacttt
    19861 ctttattttt atttcataca acactaaagc gcaatttgtt cctggcgctc ctacatttat
    19921 caaaacacct tatggtaata tttctattcc aacttattat catttgccta ttgcctattg
    19981 gaatgttgga gataaatcaa cagccatcat cacaaatgat tattacttga taaaactaaa
    20041 gaatgatagt acagtaaagg tatatggtga aattgattta tctgatagca tacattctgt
    20101 tttaataaaa gacaaaaaaa aagtagtaag aaaaatttat ccaagcgaaa caaaatatat
    20161 tgctgcttta ggcgaaaaaa atgtacatgt agctggaact ccaactgatt cgtgctggat
    20221 ctttaaacaa ttagacgaca gtgtttctct atatagtgtt gttcccagaa taggcaccga
    20281 atattctatc ttcttttcaa aaccaggaaa ttctgcatta attgcattaa atgaagccaa
    20341 tctattaaca ttggtgggca ctgacgaaaa gctagttgaa atgattaaaa acaaaagcta
    20401 tattaaagct gtcaaaaaat ataacaataa gctaaaagca aaaaaataat cttagacaca
    20461 atgtgttcaa aaattagcgt atttttttaa aaaaagtgat tagttttaaa aaaaatccga
    20521 aggaatattc cttcggattt tttcttattg tattaatttt tcccaagcta gtaatgacca
    20581 taagccaaca atagtattat gttggatata cttattatac tcgaactttt gaaatgctga
    20641 ttttgctgac ttttcggtat attcattatc atcttttaat tgccatgagc catctttttc
    20701 ttgcaatttt atcagctgca aaagcaaact ttcaaacgac tgcttataat atccggcaaa
    20761 catcatgctc atcagtgcct caactttata ctcgtcttgt atgcttacag ctgcattgtt
    20821 aattattttt tcagtaatta atgttgcata tttattttca atgtcattgt cgagttgttt
    20881 aatgtttagc ttttctttga catatgcgat ggcaaaaagt aaatgcgtta actgatacgc
    20941 ttctttgttt gttatcgaat ctgtagaata tcgattatac agtgtttgta gattcgtatt
    21001 aaatatttct aatttaaaat aaggacaatt acaatacaag ctgtaaaaca ttaaattgta
    21061 aggattgtta tagttattat ccaaacgaga tgactcgata catgtatctg ctttataaaa
    21121 aggtgacaca atttctatct cacttctatc tgttgatgac aaattctgaa taaatgattc
    21181 tgcttgtaag tctaagccta aattataata cgaattcaag tagcatagcg taacaaaatt
    21241 atatttctcc aactttactt tatcattcat gagataatta attgcttttt tcttcttaga
    21301 caatatatca ctctttatgt catttgcatt tgtagataaa tgcataaaac atactaataa
    21361 caataacaat ttgtacctca tattaaacaa tgatttgcta ttaattttta acaatcttgt
    21421 aattatacac attatcaaac aacataaagt ttaaaatata cgttgcagaa gacaaactca
    21481 ttgtattcag ttttacagta tttaatcctt tgctgatatc tacgcgttga atggaaatca
    21541 attggcctaa aatatcataa acattcatat tgatttgtct gtcttcggta gataaaatca
    21601 acacatttaa ttcttcatta acaggatttg gataaactgc caccacatta ttgctttgcg
    21661 tattatgttg tatgttaata gaaattacat ttgaatattt atagctatca tcattgtcga
    21721 taatttttaa tcgataatag ttattgcctt ccacaggtgt ttcatcaaac aactgatagt
    21781 tcaaagtagc attgctattt ccagcagctg gaacttcacc taaatagaac caagttgtac
    21841 cattaatact tttttccact acgaatttat tggtattaat ttcggtagat gttacccatt
    21901 caagtttatt tctttcacca atattttgac cagtaaagga taccaattct actggcaaca
    21961 cttcgtaagc ataaataatt ggatgaacat agaatgtaga gaaagtatca acaataaaag
    22021 acacttcgta atcgccacca tacaaagttg tgtaagctgc aaatgtagga ttagtccaag
    22081 tagcattacc tcctagagaa ggaccgccag gcaacatgat accaccagga atattgtttt
    22141 gtgttttcac attatctggt ccattgaaaa actgtccgcc tccaccaggc cattttgaca
    22201 catgtaaatc tgttgctgta ttaaaggaat aacgtgttga attggcacca gtttttaatt
    22261 tattatattc atttccagta aagtaaaaag ttactttagc atgtacattt ggcacatcag
    22321 gatttataga ccaataacga cctaaataag gcattggccc agcaacagga ttaatcgtat
    22381 ttaccaatgg aactggtgat gtattcggcg ttggaatgcg taaacaaact tcagtatttc
    22441 ctaatgctcc agttgaatat ggaccaccag ttaggtcttg aattttacaa ataattctgc
    22501 ctaaataatc atggaaataa taggtatttc cgttttgaat agaacaagta taacaaccta
    22561 aggtatccat acttatagaa ttgagtgaat ctgcaggcat gactatattc aaatttgtaa
    22621 aatcagaaca agcaccattg gtaactgtcc aacgataagt tgtagtatta gttcctaatg
    22681 gaatattaga gacatttaag gttgtactgc ttgtaggaac tgctggtgta gttgttgcag
    22741 tgcctgagac atacgtccac tgtcctgtgc cagctgctgg tgttgtagca gctaatgttg
    22801 cagttcctgg attagtacaa gaggcaatat taacaatagc agcaatagct tgtactgtat
    22861 catcaacaat ccattttaat gtatcagttg aaaatgctgt atcacaatca atagtacctt
    22921 tacattcata tgcattaaca agcattgttg taccaattac tttagaaatt gttggaagag
    22981 tcgatgacca tgctgtccaa gttacgccac catctgtagt atatcggtat atcgtattac
    23041 atgaagcacc aacgcctcca ctacttggcg aagcacctgt aggcgcagtc gctgttaaac
    23101 ttgctgcatt ggaaatacaa atattactta cattcggatt tttcgtccat gtcgtatgtg
    23161 gtgttaccgc tggatacact acaaattctg cataggaata gatagaccag ccacaacatt
    23221 tatcttttgt tctaaacctc acgatataag taatagaatc tgatgttgca ttattaaaag
    23281 taataggagt tggtgctgca cctgttacag ttgtccaagt aaatggacct ggaatcaatg
    23341 tagtattgat tttgtattgt ctatagctcc attcaaagtc agcatctgtt ccacttgcat
    23401 tcgtaaaatt attcgaagca attaatttga aagtgtcttt ttctccagag catattgcat
    23461 gcggtacaac ttttatttgc ggaacgtttc ttgcttgatc tacgaagatg acatcactat
    23521 aatattgagg accaactaca gatatatctt ttgcatcatt tcctgaaatt actgttgtat
    23581 aataaatatc tagtgcattc gatgccattg taaatgagct tgtagtagca tctacatcat
    23641 tgacaattgc accaccagaa ccaagattta gccaatttgc gccagtagtt ttacttattg
    23701 gtatgataga attcgtgcac cctttattgt attttacagt agtagtacct gctattaacc
    23761 cattattact tgaattaacg actgcactac cttgtgcatt actagcaatt gataaaccat
    23821 tagcgccagg ctgaccgtta ccacctttgc caccattgcc tcctctaccg ccttggccac
    23881 cagcacctac ctcacaatta ccgcatgttc tgccagtagg acagccagaa gtaccatcaa
    23941 caccgccact taatacatta gctccagtta atgaagtatt attaccatta cctccacaac
    24001 cgccagtacc accattgcca ccactgccac cagttcctaa tgtaccgcct gctctaaagg
    24061 caccagcagt tagtgttaca ttaattaaag tacccgtacc tgcgccatag gcatatagtg
    24121 caaatgagga gccactaccc caaccgccag tacctaaagt accaccttgg ccaccaccgc
    24181 cgccgccgcc gccgccgctt ccacccccag gtccagcgcc gcacgcatca cctcggtctt
    24241 gtaggtgtcg accg
//
LOCUS       DAOQQV010000028        23909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_635_length_23909_cov_8.247673, whole genome shotgun sequence.
ACCESSION   DAOQQV010000028 DAOQQV010000000
VERSION     DAOQQV010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23909
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_635_length_23909_cov_8.247673"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLL59_04710"
     rRNA            <1..495
                     /locus_tag="PLL59_04710"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            576..686
                     /gene="rrf"
                     /locus_tag="PLL59_04715"
     rRNA            576..686
                     /gene="rrf"
                     /locus_tag="PLL59_04715"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            784..1539
                     /locus_tag="PLL59_04720"
     CDS             784..1539
                     /locus_tag="PLL59_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HMY23140.1"
                     /translation="MFQELSLLHFIYIWIAIAIILFPIQLFVSAPYGRHTKTTWGPMM
                     SNKLGWIMMEAWALLTFWSVYFYFFNNNSYSLFFAILYTLHYIHRSFIFPLRTHTKGK
                     QMPVFIALSALIFNSINASLIAYYLSDVSVYPENYFIKWNFVVGLILFVCGFLGNYKS
                     DDILINLRKPGEKGYKIPSGFLFKYISCPNHFCEIIEWLGYMLMLSNIAGIAFFVWTV
                     ANLLPRALHHHRWYKQNFKDYPSDRKAVLPYII"
     gene            1605..3410
                     /locus_tag="PLL59_04725"
     CDS             1605..3410
                     /locus_tag="PLL59_04725"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HMY23141.1"
                     /translation="MYILVINCGSSSIKADVIDTQLKKSILELDAERLPSTPFIQLNN
                     EQIIYHGDLNFESIIRYCLHTIKSRLAEKQINGIGHRVVHGGDQYSQTVCIDAAVEAA
                     IENLADLAPLHNPANLAGIRSAKEVFPAISNIAVFDTAFHQTMPKRAKQYAIDKNIIA
                     KYNIKRYGFHGTSHKYIMERSGEYLKQDVKNLRILSCHLGNGASICAIEFGRSVETSM
                     GLTPMEGLMMGTRSGDIDPGIITYLQNKEKWTSQETEDFLNKQSGLAGLSGLTNDMRD
                     ILQQASEGNEDARTAIQVFTHRITKYIGAYAAIMSGIDVLVFTGGIGENSADIRHRIC
                     QGLEFLGIMIDDDKNKSAKLNSDNDVANISDENGRVKILAIRTDEQVAIALETQKIIQ
                     EKNKVNTIPKIPIAISARHVHLSRATVDILFGKNYELTEFKPLSQPGQFAANETVTLI
                     GLKNKIENVRILGPLRPKDQVEISKTDEFFLGIDAPVRESGHVEGSPGITLKTAHGEV
                     AIKEGVIVAWRHIHMHPNDAALFGVQDKDIVSVDVDDDERPLTFKNVLIRVSDKFKLE
                     MHIDTDEGNAAEIKKGEEGELVLSAKSVNLHDKNV"
     gene            3429..3842
                     /locus_tag="PLL59_04730"
     CDS             3429..3842
                     /locus_tag="PLL59_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23142.1"
                     /translation="MEKPSTQLEAFLFYMEKMDVEMIDLILDDDKTYEDVSKSDFISM
                     LDMAISYIKVNGGEQLVAYAGFCDSKNCPNACKSAYSFVSKKTMQRIDLVTEVKAGKI
                     LDIYTCYDINIFDKIPNFKEFEFVPLIDERDFDIF"
     gene            3929..5932
                     /locus_tag="PLL59_04735"
     CDS             3929..5932
                     /locus_tag="PLL59_04735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HMY23143.1"
                     /translation="MQHLLFVLISILLSSTIFADGDVVGKNIDKQRKPGDDFFKYANG
                     NWIKQNPIPAAYSQWSIGNLVQEDVWTKLKTINENALLSTSNDDNTRKIRDFYFAAMD
                     TLSIEKVKLNPLNKELLQINAIKTTNDLLKVITHLHQIGVPVAFDLGIYQDMKNSEVN
                     ALYLSQGGLGLPNRDYYFNKDAQTLKVANDYKTKHLVRMLAYLNPNFSSNATAVYSLE
                     KQLATKSRKLEDLRDPYENYNKMSIASLNKLTPKINWNTVFSAMSIQTDSVIVGQPEF
                     FIQLNKLLSNTPLSTWKNYLRFHLLSNYAGYLHKKVNNEDFRFYGKVVRGTKEQLPRW
                     KRALRWEEEAMGELLGQLYVKDNFSPKTKDRYEKLCDEVFAAFATRIKNLDWMSETTK
                     QKSLLKLSTVTKKVGYPNTWKDFSALKTNDKSLVENIQQANKFWFDYAVSKLNKPVDR
                     TEWDMTPQTYNAYYNPSNNEIVMPAGIFVIPGFKDEEIDDAVIYGYAAASTIGHEITH
                     GFDDEGRQFDDKGNLQNWWLEEDEKKFNEKAQKYVEQFNNYIVLDSMHINGKATLGEN
                     IADLGGLVIALDAFKKTNQYKEGKLIDGLTPLQRYFLGYALGWLGHQRDEELATRVLT
                     DVHSPAFLRVNGPFSNIDEFFEAFGIKAGEKMYRPEAERVKIW"
     gene            6360..6770
                     /locus_tag="PLL59_04740"
     CDS             6360..6770
                     /locus_tag="PLL59_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23144.1"
                     /translation="MKNIYKILSLFTLLLIVLAPFQLLANSHDTDKNSEPIVKSIWKY
                     EQDQTKYSPEDGIAKKIFYLSLNSDGTYHQYVQVIPSSPRDYFKVKNYSIDGKWTEKN
                     GKLILIEYGKENAIDYNFLYTNFENIDNQVVKVK"
     gene            6914..9853
                     /locus_tag="PLL59_04745"
     CDS             6914..9853
                     /locus_tag="PLL59_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HMY23145.1"
                     /translation="MKQEVEISHFEQLKASLEGELYDDKTMRTLYATDASVYKEYPLA
                     VCYPKSDQDIQKLIQYALQHNTSLIPRTAGTSLGGQVVGNGIVVDMSKHLNKLITVNK
                     EEKYCIVQPGMVRDELNRQLSATGLFFAPETSTANRAMIGGMVGNNSCGTNSIVYGST
                     RDHVIALKGFLSDGSAFDCSPINAADFHQKSQQDNLEGSIYSFLQNELSDAATRDEII
                     SQFPNKKIHRRNTGYAVDELLYLQPFTKEGDAFNLSKLICGSEGTLAFITEIKLNLVP
                     VPPKEKVVVAIHFKTLEESLRATVIAMRHQPSACELMDKIILDCTKQNIEQNKNRFFV
                     KGDPEAILCVEFHANSKEEIEQKAKQLQQDFENENVGYHFPYLFGADINKVWNLRKAG
                     LGLLANIPGDAKAVAVIEDTAVAVEDLPNYIADFTKMMDSHQQRSVYYAHAGAGELHL
                     RPILDLKKQNDRVLFRTIATETANLVKKYNGSLSGEHGDGRVRGEFIPLMVGEKNYQL
                     LRRLKYTFDKHNIFNPGKIVDTPPMDTSLRYEENQVTPQFDTVYNFDKDEGILRAAEK
                     CNGSGDCRKLPGSGGTMCPSYMATRNEKDTTRARANILRDFLSNSTQPNKFNHKEIYE
                     VMDLCLSCKGCTSECPSNVDIPTLKAEFLHQYYKDNGVPLRTKAIANIGQLSKLAMLF
                     PALSNFGLSNKYTSKILKKILRIAPERSMPLYYKETLLSWYKKNSKELAIKNEQKGKV
                     YFFFDEFTNFNDVEIGIKALKLLTKLGYEVDYIEHVDSGRAHMSKGLILEAQKLAKTN
                     VSIFSTWINKDVPLIGIEPSAILSFRDEYPRLVERKDVETAKKLAKNCFIIDEFIAQE
                     IEKGNITAKQFTTATKMIKLHGHCHQKALSSINSSIQLMSLPENYHVENITSGCCGMA
                     GSFGYEKEHFEVSMQVGELVLFPAVRNAPAETIIAAPGTSCRHQIKDGTQKHALHPIE
                     VLYEALL"
     gene            9985..10917
                     /locus_tag="PLL59_04750"
     CDS             9985..10917
                     /locus_tag="PLL59_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMY23146.1"
                     /translation="MQSIQIYTVKVFINVVRNILFVHKTEPNTVRVAFERLSNITKYP
                     RFVKKEELNYAGCEAAWFTPDGYIKSKTILYLHGGGYVVGSYNTHRALIARIARAAAC
                     KTLAINYRKAPEHHYPLAIEDALNTYKQMIADGYENIFIMGDSAGGGLTLALLQLIKK
                     HQLPKAAGSVLLSPWTDLTMSGDSIRTKKDVDPLITPQLIEIFAKRYFAEADPKDPLI
                     SPLFADVSDFPPTLIQVGTNEVLLDDSTRLAKKMNDAGVPVQLEIYKNMMHVWQYLGG
                     IVPEANKAIDEIGAFVKSISVSTTSDNLDRANIY"
     gene            10946..11449
                     /locus_tag="PLL59_04755"
     CDS             10946..11449
                     /locus_tag="PLL59_04755"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HMY23147.1"
                     /translation="MKRIFILLTSILVFSCNQNAKTNQDEKLETEPNTKSETKMTENL
                     DWLTGNWKRLNEEAGKETFENWKKISPSEYSGIGFTIQKGDTISQEKMDIIETSGKWT
                     LFVKMPNDKEATKFEMTELKYNEFVCVNDSIDFPKKIKYWTDGEKIKAKISNEEMEIP
                     FEFSRIK"
     gene            11642..12130
                     /locus_tag="PLL59_04760"
     CDS             11642..12130
                     /locus_tag="PLL59_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23148.1"
                     /translation="MKNFIVLFVLLIKFSFILGQNCIDSLSDNNVLILKNPNSILKFA
                     SIKNNKSDFFLLKCKDNKISSEYLNSFKEKSLCIWNDKKLKRNKRSIIANSVIGGVLL
                     IPVFATIKNLRNDREPIGPLITIFSLAPISSTFGITAIGLGIKRAVILKKSKKNILKC
                     NF"
     gene            12430..13446
                     /gene="metX"
                     /locus_tag="PLL59_04765"
     CDS             12430..13446
                     /gene="metX"
                     /locus_tag="PLL59_04765"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HMY23149.1"
                     /translation="MSTQILTHHEAIQLECGITLPKVDIAYSTFGEMNADKTNVVWIC
                     HAFSANSNPEEWWPGMVGEGLFLDPKKYFIVCANMLGSCYGTTGPLSENPVTGKKYYK
                     DFPTVTVRDMVQTLTILRKHLGIEKIFFACGFSMGGQQLLEFVIQEPDIFENILLGAT
                     NIQHSPWGIAFNESQRMAIEADTTFGDDSDDAGLNGMKAARSIGLISYRNYKAYNITQ
                     AEPDNNKLENYKACSYQRYQGEKLAKRFNAYSYHILSKAMDNHNITRNRGNAKDVLSA
                     VKCNVLVLGITSDYLYPLEEQQLLARLIPHSMFVEIDSDYGHDGFLIENEQMSKILSA
                     FLKV"
     gene            complement(13447..14280)
                     /locus_tag="PLL59_04770"
     CDS             complement(13447..14280)
                     /locus_tag="PLL59_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23150.1"
                     /translation="MTTTNIAIASTSGHEIMLTVRTPNDNIKGIVQINTGTCIPQKIY
                     WKFAEYMTKNGYVTITYDYTDADNYQSNVTHAVWLKDIESVTAFIIDNYPTLKKYAVG
                     HSSGGQFIGYTKNATAFEKIYLVACANGYVKNLSLAMRIAIFFLWKIIVPFSIWKYGY
                     MNNAAFGISGGFPKQIILELRSWCYRPDFFTSYFEEKKIPSYYHTITAPVKAFHLADD
                     AIANRKSCRFILDLYSQADRSIETLYAADYDIKKFGHRGFFFSSAEKLLWPKFLKEME
                     S"
     gene            14450..15967
                     /locus_tag="PLL59_04775"
     CDS             14450..15967
                     /locus_tag="PLL59_04775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMY23151.1"
                     /translation="MTRKTIILIGFIGLKFLLQYFLISSEYELQRDGFLHLDQANHLA
                     WGYLSVPPFTSWISYIIQMLGNSIFWVKFFPALFGALTIYVVWKAIEELGGNLFALIL
                     GANCVLFSALLRLNTLYQPNSFDVLSWTVFYFILLKYIKTDDDKWLYIGATVFALGFL
                     NKYNIVFLLIGLSPSLLLTEHRKIFLKPTLYFAILLGLLLILPNLIWQYNNNFPVFKH
                     LKQLAQIQLVNVNRFDFLKNQLLFFIGSLIVIFSSFYAFFFYKPYQKYRIFFFSIIFT
                     LTAFLYFKAKDYYAIGIYPIYIAFGSVFLADVLKGARKKFLQPIVIVIPLLLFIPIYN
                     VAFPNKSPAYIIQHSDNYKKLGMLRWEDGKDHSLPQDFADMLGWKELAQKVDRIYLSL
                     ANKNNTLILCDNYGQAGAINFYTNQKLRAVSFNADYIDWFDLTKKYENLISVKEAEEG
                     SVEFQETAPFFLHSSLADSISNQYAREFGTRIFVFKGAKIDIRQRIKNEIDENKN"
     gene            16030..16725
                     /locus_tag="PLL59_04780"
     CDS             16030..16725
                     /locus_tag="PLL59_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HMY23152.1"
                     /translation="MIPTIVHDFYKLFYPAICGGCDTTLAKGEQQLCFHCRLALPFTQ
                     FEKIQNNPVEQLFFGRVPIEFGTALLYFSKAEMVQQIMHNIKYNDKKDLAQLLGKILG
                     ERLQNIASLHDVTAIVPVPLHERKLRMRGYNQSECLAEGINEILKVDIVVNGIQRIKH
                     TDSQTNKNMIERWENVKDAFEIKQIEKLKNKHILLLDDVLTTGATLEACALTLIEKAN
                     CKVSIATLAFVAD"
     gene            16757..18238
                     /locus_tag="PLL59_04785"
     CDS             16757..18238
                     /locus_tag="PLL59_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23153.1"
                     /translation="MKRTAIIFEKFIAYRMLLLFGVLFIFSFTACKKEQLNTSSGISF
                     STDTLSFDTLFSTLGSTTRFFTIKNTHKQAIKISNIQLAGGITSPYRINVDGDAGTSF
                     NDIEIPAKDSIYVFVEVTINPTAANLPFIVEDSVQFQTNGILQQVQLNAYGQNAHFFN
                     GDSIENNTVWNDDLPYVILNYLQIKPGASLSIQAGCDVYFGSNAALLVEGDLNVNGTD
                     TSNMVTFRGVRLDKDIADRSYDDFPGQYAGVFFLRNSNGNIEYLNMRNSLYGINVGNI
                     KTSDDPDENLAALQAMSISNAPTLTIKNSKIYNHAFYGLFGFLGKIYAENTLIYNCGK
                     NVVGLFDGGDYQFLNCTFYNRSSAYISHAKEPLLYMNDYFKIGDNTYVIADAATAVFE
                     NCIVSGTLEEEIIKDDITQNPLPISLSFDHCVVKTKEPLNAPTFLNCKNENPIFKDIF
                     KNDFHLKSTSPCVGFSTSNFPIIDIDGFARANTPTDCGAYIFQ"
     gene            18298..20556
                     /locus_tag="PLL59_04790"
     CDS             18298..20556
                     /locus_tag="PLL59_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23154.1"
                     /translation="MKIIFSKFLHNIYYSFPIQLFILHWNKHLFVLLFWIILFLSVSQ
                     SFGVSYGIPLLFLDPEYVGHVNFLSFVIVGFSMGGFIMAWNMSFYVLNSYRFEFLASQ
                     VNPFVQFCWNNFIIPFSFVIVYIVQLVKFQHAEALLPAGKIVANVIALLCGNLLMIIV
                     SSLFFVLFNHDVETFLAKLSDKAKTSLLGKKIHLDKLEFGTTSAAQWQVEKFIAFPFS
                     IKTVRNADFYDKKLVAKVLSHHHRNAILFQCLAICGLLLLGKLMDNPYFRLPASSAIF
                     LIFSVLVVFFSFLSFWFRTWRTVVVIVFFFILNILTQYDLVVYKHKLYGLNYSAQKIS
                     YNNEVINNAVHKPLVDADIIATKKILENWKKKMHDKYGETKPKMLFVNTAGGGLKAAY
                     WTFYLLQQLENKTQQKLFDHTMLMTGASGGMIGAAYFRELYLQQATRPSVNYLDTTYL
                     YNIGLDLLNGMATSIATNDIFYPWQSYSYGGYKYKKDRAYMFDKKLNENTNFVLSKLL
                     LDYKQPETKAQIPMMILSATIINDQRFLFFSPQSISYLIKPYIKNSKGYVDDMSTDAI
                     EFRRFFKTQGADSINFIDALRTNATYPYIMPAVYLPTTPEIKAMDAGIRENSGLAVST
                     RFYSVFKDWIDANTSGVVFLSFRVDNKLHDFTAQKKQTYLSELLSPVGSIFNNFILLQ
                     DYNSDVSLAYLENASSTDINVLNFNYDQTSKRKKASMSWHLTTDEKRDIKSAFYQKNN
                     QLMLEKLNELLQ"
     gene            complement(20563..21972)
                     /gene="rlmD"
                     /locus_tag="PLL59_04795"
     CDS             complement(20563..21972)
                     /gene="rlmD"
                     /locus_tag="PLL59_04795"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HMY23155.1"
                     /translation="MRRKKKVEYPIIENVAVTAIAAEGKGLARVDNFVWFIDKAIPGD
                     VIHAKVTKKKKDFGVAEIDTLVEASAHRKDFFCEHFGTCGGCKWQHVDYAYQVEMKHQ
                     LVLDAFRRIGKIELENVEPILACEATEFYRNKLEFTFSNRVWLTREQIDSGESFNRNA
                     LGFHTAGNFASILHINTCYLQDEKVNTIRNAVYSFAIQHQYTFYNLKHHEGLMRNLIF
                     RSSTLDEMMVTVCFAENKMPEITAMMDYLQNTFPYISSLNYIINTKKNDTIYDQDVIC
                     YNNKNHIIEQLGNIKYKISPKSFFQTNSRQAKRLYDLVKEFGGFSKNDFVYDLYCGTG
                     SIALYIADDCKKVLGIEQIPDAIADAKENAALNQIENCDFYVGTCEDILKPEFIAVHA
                     TPDIVVLDPPRAGLHEKVVNVLLEAAPKKIVYVSCNPSTQARDVLLFSEKYKVTRCRP
                     VDMFPHTFHIENIVLLELI"
     gene            22074..>23909
                     /locus_tag="PLL59_04800"
     CDS             22074..>23909
                     /locus_tag="PLL59_04800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMY23156.1"
                     /translation="MKKALLTIFAISSFAITTAFSQSETLFTVGDTKVKKSEFEYIYK
                     KNNFSNKADFSKKSLDDYLNLYVNFRLKVKEAMAQGLDTGSRFKEELTVYEKQLLESY
                     VEKDVLDKLVKQEFERSKTDVQLSHIFFSNSNGDEQALEKANEAYAKLKAVAAFSEVA
                     KTSDDKSTASQGGKVGWMNSYQMTYPQMEEIAYSLKTGAYAKPVKTQLGYHIIQLDAT
                     RPARPKIKAAIIKRFLPIADTSAAAFKQTEDSMKMAYAALKSGAAFEKIVERFSEDDA
                     SKSNKGELDWFGINTYAKIFEETAYALQDGEYSLPFKTKTAWYIVKRVQTAKPLTIET
                     ATPILKTKLPSLPIYQYEMDKFIQTISNKFSVQELTENINSFKSRITVLANTSPFKYR
                     DTASTKPLLKIGNSTFTENDFGKKFQDIFYTVYPKPGAEKFDVLYKNTLQAFIIDYYK
                     QFVKENNAEYKSLMDEYKNGIMIFALSENNIWNKASSDSVGLLAYYKEHQQDFNLKKR
                     ASVRTISAANSKQINTIYKLLLANPTISDDSLLTVMKSIGLTSAQLKLATADSTQKKW
                     NINKESLSLPKYVGNSYTITQVYNLQAAKTRHLDECRGYVVAAYQE"
BASE COUNT         8231 a         3832 c         4280 g         7566 t
ORIGIN      
        1 agtgatccgg tggttctgca tggaagggcc atcgctcaaa ggataaaagg tactccgggg
       61 ataacaggct gatctccccc aagagctcat atcgacgggg aggtttggca cctcgatgtc
      121 ggctcgtcac atcctggggc tggagaaggt cccaagggtt gagctgttcg ctcattaaag
      181 tggcacgcga gctgggttca gaacgtcgca agacagttcg gtccctatct gttatgggcg
      241 tttgagaatt gagaagatct gtctctagta cgagaggacc gggatggact gacctctagt
      301 gtaccggtta tgtcgccaga tgtaccgccg ggtagctacg ttgggaaggg ataagcgctg
      361 aaagcatcta agcacgaaac ccacttcaag atgagttctc atagggtcgt cgaagactac
      421 gacgttgata ggtcataggt gtaaagatgg taacatcaaa gccgagtgat actaataacc
      481 catcaacttt ttaaaatttg tataatgatt tcccaatgtg ttaaaattta ctgacaaaca
      541 agtcattaaa atataaggta ttttaccaaa gatttaatgg cgattatagc gtaggtgttc
      601 acctcttccc attccgaaca gagaagttaa gccctacagc gccaatggta ctaggttaaa
      661 cctgggagag taggtcgtcg ccaaccctta caagccgtct cgtgaataac gaggcggctt
      721 ttttatttta aattcacctt tttccattac tttatttatc tctttgatgt ttacttttgt
      781 atgatgtttc aggaattaag cttattgcat tttatataca tttggatagc cattgctatt
      841 attttatttc caattcaatt gtttgtttct gcgccgtatg gaagacatac gaaaactact
      901 tggggaccaa tgatgtcaaa taaattaggt tggataatga tggaggcttg ggcattactt
      961 actttttgga gtgtctattt ttattttttc aataacaact cttactcctt attttttgca
     1021 attttataca cactacatta tatccatcgt agttttattt ttccattacg cacccataca
     1081 aaaggcaagc agatgccagt atttattgca ctttctgcat taatatttaa ttcaataaat
     1141 gcgagcctaa tagcttatta tttatctgat gtgagtgtgt atccagaaaa ttattttata
     1201 aaatggaatt ttgtagtagg attaatattg tttgtttgtg gctttttagg aaattataaa
     1261 tcagatgata tattgattaa tttaagaaaa ccaggtgaaa aaggatataa aattccatca
     1321 ggttttttgt ttaaatatat ttcttgccca aaccattttt gcgagattat agaatggtta
     1381 ggttatatgt tgatgctgtc gaatattgca ggtattgctt tttttgtatg gacagtagct
     1441 aatttattgc cacgagcatt acatcatcac cgttggtata aacagaattt taaagattat
     1501 ccttctgata gaaaggctgt actgccttac attatatagt tttttaagaa aatagttttt
     1561 cattctacat tgcttattta ttatcttcga ccaaaatttt ttgtatgtat atattagtta
     1621 ttaattgtgg tagttcgtct ataaaagctg atgttattga tacgcaatta aaaaaatcga
     1681 ttttagaatt agatgcagag agattgccat caacaccttt tattcaattg aataatgaac
     1741 aaattattta tcatggtgat ttgaattttg agtctattat acgatattgt ttacatacaa
     1801 taaaatcacg cttagcagaa aaacagatta atggtattgg tcatcgtgta gtacatggtg
     1861 gcgatcaata tagtcaaacg gtttgcatcg atgcagctgt agaggctgct atcgaaaatt
     1921 tagcggattt agcaccattg cataatcctg caaacttagc tggtattcgc agtgcaaaag
     1981 aagtatttcc tgcaatttcc aatattgctg tctttgatac agcatttcat caaaccatgc
     2041 caaaacgtgc taagcaatat gctatcgata aaaacataat tgccaagtat aatattaaac
     2101 gatatggttt tcatggtact agccataaat acattatgga aagaagtggt gagtatttga
     2161 aacaagacgt aaaaaactta cgtattttat catgtcactt aggtaatggt gctagtattt
     2221 gtgcaataga atttggtaga agcgtagaaa cgtctatggg tttgacgcca atggaaggct
     2281 taatgatggg cacgcgtagt ggtgatattg atcctggaat tattacttat ttacaaaata
     2341 aagaaaaatg gacgagccag gaaacagaag attttttaaa taagcaatca ggacttgcag
     2401 gcttaagtgg tctgacgaat gacatgcgtg atatattgca acaagctagc gaaggcaatg
     2461 aagatgcacg cacagcaatt caagtgttta cacacagaat tacaaaatat ataggtgcgt
     2521 atgcggcaat catgagcggt attgatgttt tagtatttac tggaggcatt ggtgaaaata
     2581 gtgctgatat cagacataga atttgtcaag gattggagtt tttaggaatc atgattgatg
     2641 atgacaaaaa caaatctgca aaattgaata gtgataatga tgttgccaat atttcagatg
     2701 agaatggacg tgtgaaaata ttagcaattc gaactgacga gcaagttgcc attgccttag
     2761 aaacacaaaa aataattcaa gagaaaaaca aagtaaatac gattccaaaa attccaattg
     2821 caatatctgc aagacatgta catttaagca gagctaccgt agatatttta tttggaaaga
     2881 attatgaact tactgaattt aagccattgt cgcaacctgg acagtttgcc gccaacgaaa
     2941 cggtaacact tattggtcta aaaaataaaa tagaaaatgt tagaatttta ggaccactac
     3001 gtccaaaaga tcaagtagag atttcaaaaa cggatgagtt ttttcttggc attgacgcgc
     3061 ctgtgcgcga aagtggacat gtcgaaggtt cgcctggcat tacactaaaa actgcacacg
     3121 gcgaagtagc cattaaagaa ggtgtaattg ttgcatggcg acatattcac atgcatccga
     3181 acgatgccgc tttgtttggc gtacaagata aagatattgt aagcgttgat gttgatgatg
     3241 atgagcgacc attgacattt aaaaatgtat tgattcgcgt gtcagataaa tttaaacttg
     3301 aaatgcatat cgatacagat gaaggcaacg ctgcagaaat aaaaaaaggc gaagaaggag
     3361 aattagtgtt gagtgcaaaa agcgtcaatt tgcacgataa aaatgtatga ttttttaagc
     3421 tattcaagat ggaaaaacca agtacacaac tagaagcatt ccttttttat atggaaaaaa
     3481 tggatgtgga aatgatagat ttgattttag atgatgacaa aacctatgaa gatgtgtcta
     3541 agagtgattt tatatcaatg cttgatatgg caattagtta cataaaagta aatggtggag
     3601 agcaactcgt cgcctacgct ggtttttgtg atagcaaaaa ctgtccgaat gcatgcaaga
     3661 gtgcatattc atttgtcagt aaaaagacaa tgcaaagaat agaccttgtt acggaggtaa
     3721 aagctggaaa aatattagat atatacacct gttatgacat caatattttc gacaagattc
     3781 caaactttaa ggagttcgaa tttgttccac ttattgatga acgtgatttt gatatcttct
     3841 aaattaattt agcttgatta aagtaattga attcttttgt aataaatcaa gctgttttta
     3901 tactaatttt ataaccaaat aagctaacat gcaacattta ctttttgtac ttatttcaat
     3961 attactatct tctacaattt ttgcagatgg cgatgtagtc gggaaaaata ttgacaaaca
     4021 aagaaaacct ggcgatgatt tttttaaata tgctaatggc aattggatca agcaaaatcc
     4081 aattccagct gcgtattcgc aatggtcgat tggcaatcta gtgcaagaag atgtttggac
     4141 aaaattaaaa acaatcaatg aaaatgcact tctgagcaca tctaatgatg acaatacgcg
     4201 aaaaattcgt gatttctatt ttgctgcaat ggatacactc agcattgaaa aagtaaaatt
     4261 gaatccactg aataaagagt tattacaaat aaatgcgatt aagacaacga acgatttgtt
     4321 gaaagtgatt acacacttgc atcaaattgg tgtgcctgtt gcttttgatt taggcattta
     4381 tcaagacatg aaaaacagcg aagtgaatgc gctttatctt tctcaaggag gcctcggttt
     4441 gcccaataga gattattatt ttaataaaga tgcacaaaca ttaaaagtgg caaacgatta
     4501 taaaactaag catttggtgc gtatgcttgc ttatttaaat ccaaattttt cgagtaatgc
     4561 aacagctgtt tatagcttgg aaaaacaatt agcaacgaaa tcaagaaagt tagaagactt
     4621 gcgcgatccc tatgaaaatt acaataaaat gtccattgca tcgcttaata aattgacgcc
     4681 aaaaattaat tggaatacgg ttttttcagc catgtcaata caaacagatt ctgtgattgt
     4741 tggtcagccc gaatttttta tacaattaaa taaattacta tcgaatacgc cattatcaac
     4801 ttggaaaaat tatttacgat tccatttatt gtcaaattat gcaggctatt tacataaaaa
     4861 agtaaacaat gaagatttta gattttatgg caaagtagtt cgaggaacaa aagagcagtt
     4921 gccacgctgg aaacgtgcac ttcgctggga agaagaagcc atgggcgagc tgctaggaca
     4981 gttatatgta aaggataatt tttcgccaaa aacaaaagat cgctacgaaa aattatgcga
     5041 cgaagtgttt gctgcatttg ctacgcgcat aaaaaattta gattggatga gcgaaactac
     5101 aaagcaaaaa tctttgttga aattaagtac ggttacaaaa aaagtaggat atcctaatac
     5161 atggaaagat ttttctgcat taaaaaccaa tgacaaatct ttagtagaaa atatacaaca
     5221 ggctaataaa ttctggtttg attatgcagt gagcaaatta aataaaccag tagatagaac
     5281 agaatgggac atgacgcctc aaacgtataa tgcctattac aatccatcga ataatgaaat
     5341 tgtaatgcca gccggaatat ttgttattcc aggctttaaa gatgaagaaa tcgacgatgc
     5401 tgttatttac ggatatgcag cagcttctac tattggccac gaaattacac atggttttga
     5461 tgatgaaggc aggcaatttg acgacaaagg aaatttgcaa aattggtggc tggaagaaga
     5521 tgaaaaaaaa tttaatgaaa aagcacagaa atacgtagag caattcaata attatatcgt
     5581 tttagattct atgcatatca atggcaaagc aacattaggt gaaaatattg ccgatttagg
     5641 tggtttagtc atcgccttag atgctttcaa aaaaacgaat caatataaag aaggtaaact
     5701 tatagatggc ttaacgccat tacaacgata tttcttaggc tatgcgcttg gctggttagg
     5761 tcaccaacgc gatgaagaat tggcaacacg tgtgcttact gatgtacatt cgcctgcctt
     5821 tttacgtgtt aatggaccat tttctaatat agatgaattt tttgaagcat ttggtattaa
     5881 agctggcgaa aaaatgtaca gacctgaagc cgaacgcgtg aaaatatggt aaattgatta
     5941 cctcgatata aattgttttt tctaggttta taaaagatta tttcaatata acttctttgt
     6001 taacagctac ttatttgtag tgatgtaggg tttttattca taaagacgat aatttttata
     6061 gtttgccact tgctaactca atccagccac ttattgcttt agatttgcca tttaacaagt
     6121 gtcaacaatt cgcataaaaa gaagatgtaa tattgcacta acagatttgt ttacgtttct
     6181 ctctctttta atctgttgtt tacggctttt aaaaggccct aaagtattag aaatcttaag
     6241 aacccaaaaa cagtttcatt cccaacatga aacttttttt ctaaaaagat tgtttataat
     6301 agtgaacact caaatagaaa atagaactat gatttttata ctttctacaa caactgatta
     6361 tgaaaaatat ttacaagata ctgtcccttt ttacactact actaatagta ctcgcacctt
     6421 tccaattatt agcaaatagc cacgatacag ataaaaattc tgagccaata gttaaaagta
     6481 tttggaaata cgaacaagac caaaccaaat actcgcctga agatggcatt gcaaaaaaaa
     6541 tattctattt gtctctaaat tctgatggca cttatcatca atatgtacaa gtaattccat
     6601 catcgccacg tgattatttt aaagtaaaaa attattctat tgatggcaaa tggactgaaa
     6661 aaaacggaaa attaatattg atagaatatg gaaaagaaaa tgctatcgat tacaatttct
     6721 tatacaccaa tttcgaaaat attgacaacc aagttgtcaa agtgaaataa aatgcttgat
     6781 tataatgttg tttacgtgcc actgtttata cggtggcttt tttatttata aattattaat
     6841 tctactaatt tccttacact aaagtatagc attttgtaaa tttacttttt aagcatgttc
     6901 aatagaacaa aatatgaaac aagaagtaga aattagccat tttgagcaac tgaaagcatc
     6961 tttggaaggt gagctgtatg atgataaaac catgcgcacc ttatatgcga cagatgcatc
     7021 agtatataaa gaatatccgc tggccgtttg ttatccaaaa tcagaccaag acattcaaaa
     7081 acttatacaa tatgcattac agcataatac atctctaatt ccgcgcacgg caggcacttc
     7141 gctcggcggt caagtagtag gaaacggtat tgttgtagat atgtcaaagc atttaaacaa
     7201 acttatcacc gtcaacaaag aagaaaaata ttgtatcgtt caaccaggaa tggtacgcga
     7261 tgagctcaat agacaattaa gtgctactgg cttatttttc gcacctgaaa catcaacggc
     7321 aaaccgtgcc atgattggcg gtatggtagg caataattct tgcggcacca attctatagt
     7381 ttatggtagc acacgtgatc acgttattgc gctaaaaggt tttttaagtg atggctctgc
     7441 gtttgattgt tctcctataa atgcggctga ctttcatcaa aaaagtcaac aagacaattt
     7501 agaaggctcc atttattctt ttttgcaaaa cgagctatca gatgcagcaa cacgagatga
     7561 aattattagt caatttccaa acaaaaagat acatcgtaga aacacaggtt atgcagttga
     7621 tgaattatta tacttacaac cattcacgaa agaaggagat gcttttaatt tatcaaaatt
     7681 aatctgcggt agtgaaggca cattagcatt tattacagaa ataaaactta atcttgtacc
     7741 tgttccacca aaagaaaaag tggtggtcgc tatacatttt aaaacattag aagaaagcct
     7801 cagagcaaca gtaattgcta tgcgtcacca accatcagcc tgcgagttga tggacaaaat
     7861 aattttagat tgcactaaac aaaatatcga acagaacaaa aatagatttt ttgtaaaagg
     7921 cgatccagaa gccattttat gtgtagagtt tcatgcaaac agcaaagagg aaattgaaca
     7981 aaaagcaaaa caattacaac aagattttga gaatgaaaat gtaggttatc attttcccta
     8041 tttatttggt gctgatatca ataaagtatg gaatttacga aaagcaggac taggcttatt
     8101 ggctaatatt cctggagatg ccaaagctgt tgcagtcatt gaagatacag ctgttgcagt
     8161 tgaagatttg ccaaattaca ttgctgattt tacaaaaatg atggacagcc atcagcaacg
     8221 cagcgtgtac tatgcacatg caggcgccgg tgagttgcac ttgcgtccaa tcttagattt
     8281 gaaaaagcaa aacgatagag ttttatttag aactatcgca actgaaacag caaacttagt
     8341 caaaaaatat aatggttcat taagtggaga acatggcgat ggccgtgtgc gtggcgaatt
     8401 tattccatta atggtgggcg agaaaaatta tcaattatta cgtagattaa aatacacttt
     8461 tgataaacat aatattttca atcctggaaa aattgtggac acgccgccaa tggatacgtc
     8521 attgcgatac gaagaaaatc aagtaacacc acaatttgat acagtttata attttgataa
     8581 agacgaaggc attttacgtg ctgccgaaaa atgcaatggt agtggtgatt gccgcaaatt
     8641 acctggtagt ggaggcacga tgtgtccttc ctatatggca acacgcaacg aaaaagatac
     8701 gacacgtgca cgcgcaaaca ttttaagaga ttttttgtcc aattcaacgc aacctaacaa
     8761 attcaaccat aaagaaattt atgaagtaat ggatttgtgt ttaagttgta aaggttgcac
     8821 ttccgagtgt ccttcaaatg tggatattcc aactttaaaa gctgaatttt tacatcaata
     8881 ttataaagat aatggcgttc cacttcgcac taaagcaatt gcaaacattg gtcaattgag
     8941 caagttagcc atgttgtttc ctgccttgag taattttgga ctaagcaata aatatacttc
     9001 taaaattctt aaaaaaatat tgcgtattgc gccagagcgc tcgatgccat tgtattataa
     9061 agagacttta ttgagctggt ataaaaagaa tagtaaagag ctggcaatta aaaatgaaca
     9121 aaaaggaaaa gtctattttt tctttgatga atttaccaat tttaatgatg tagaaattgg
     9181 catcaaggca ttgaaattac ttacaaaatt aggctacgaa gtagattata tcgaacatgt
     9241 cgatagtggt cgtgcgcaca tgtcgaaagg tttgattttg gaagcacaaa aattagcgaa
     9301 aacaaacgtt agtattttca gcacttggat taataaagac gttccattaa taggaatcga
     9361 gccttctgct attttatcat tcagagatga atatccacga ttggtagaac gaaaagatgt
     9421 agaaaccgca aaaaaattag ccaaaaattg ttttatcata gatgaattta tagcgcagga
     9481 aattgaaaaa ggaaatatta cagctaagca atttacaaca gctactaaga tgattaaact
     9541 acatggtcat tgccatcaaa aagcattgtc atctataaat tcgtcgatac aattaatgag
     9601 cttgccagaa aattatcatg tcgaaaatat tacaagcggt tgctgtggca tggcaggcag
     9661 ctttggctat gagaaagaac attttgaagt aagcatgcaa gttggtgaat tggtattgtt
     9721 tcctgctgtt cgtaatgcac cagcagaaac gattattgcg gcaccaggta caagttgtag
     9781 gcatcaaata aaagatggca ctcaaaaaca cgcattgcat ccaatagaag tattgtatga
     9841 agcattgcta taatcaatta ttctaatgcc atttttatgc tgattatttt tatacgaatg
     9901 ttaatttttt cggtgtattt cagttaacca gcgtaatata aaaaatgctt agttttatcg
     9961 cacgtcaata aacgcattta tattatgcag agtattcaaa tttatacagt caaagttttt
    10021 atcaatgtag ttcgcaatat tttatttgta cataaaaccg aaccgaatac cgtgcgtgta
    10081 gcttttgagc gcttatctaa cattaccaaa tatccacgat ttgtaaagaa agaagaatta
    10141 aattatgctg gctgcgaagc agcttggttc acacctgatg gatatatcaa atcaaaaaca
    10201 attctttatt tacatggagg tggttatgtt gttggttcat acaatacaca tcgagcgctg
    10261 attgcacgca ttgcaagggc agcagcttgt aaaacattag caataaatta tagaaaggcg
    10321 ccagaacatc attatccatt agcgatagaa gatgcgctga atacatataa gcagatgatt
    10381 gctgatggtt atgaaaatat tttcattatg ggcgattctg ctggtggtgg tttgaccttg
    10441 gctttattgc aattaattaa aaaacatcag ctgccaaaag cagcaggtag cgtgctttta
    10501 tcaccatgga cagacttgac catgagtggc gatagtatta gaacaaaaaa agatgtagat
    10561 ccactcatta caccacagct aattgaaata tttgcaaagc gttactttgc agaggctgac
    10621 ccaaaagacc cattgatatc acctttattt gcagatgttt ccgatttccc accgacttta
    10681 atccaagtag gcaccaacga agtattactc gatgattcta ctcgattggc caagaaaatg
    10741 aatgatgcag gcgtgccagt tcaattggaa atatataaaa acatgatgca cgtttggcaa
    10801 tacttaggtg gcattgttcc agaagccaat aaagcaattg atgaaattgg agcatttgta
    10861 aagtcaatta gtgtcagcac aacatctgat aatttagatc gcgcgaatat ttattagtta
    10921 caggcaagca aaaatcaaaa ctcgtatgaa aagaatattt attttactga cttcaatttt
    10981 agttttttcc tgcaatcaaa acgctaaaac taatcaagac gaaaaattag aaacagaacc
    11041 taatacaaaa tctgaaacaa aaatgactga aaatttagat tggttaaccg gaaattggaa
    11101 aagactgaac gaagaagccg gaaaagaaac ttttgaaaat tggaagaaaa ttagtccaag
    11161 tgaatactct ggaattggat ttacaattca aaaaggagac acaataagcc aagaaaaaat
    11221 ggacataatc gaaactagtg gaaaatggac tttatttgtt aaaatgccaa acgataaaga
    11281 agcaacaaag tttgaaatga ctgaactgaa atacaacgaa tttgtttgtg ttaatgattc
    11341 tattgatttc ccgaagaaaa ttaaatattg gactgacgga gaaaaaatta aagcaaaaat
    11401 atctaacgaa gaaatggaaa ttccttttga gttttcaaga attaaataaa gataaattgc
    11461 ctgcatataa caatcggttt tgcgtcaggc ggggtaaacg tcttcgattg aacaattttt
    11521 actaatttta ggatgtcggc ttcgattaac gttttgtgct ggaaatttcg cccgaacgca
    11581 aatccgccag acgttgatga taatgctcta aagactgtat ttttaataaa cattcttaat
    11641 tatgaaaaat tttattgtac tatttgtatt gttaataaaa ttcagtttca tactaggtca
    11701 aaattgtata gattctttat cagataacaa tgtattaatt cttaaaaatc ctaattcaat
    11761 actaaaattt gcatctataa agaataataa atctgacttt tttcttttaa aatgtaaaga
    11821 caataaaatc agttctgaat atctaaattc atttaaagag aagagtttat gtatttggaa
    11881 tgacaaaaag ttaaaaagaa ataaaaggtc aataattgca aatagtgtaa ttggaggagt
    11941 cttattaatt ccagtttttg cgacaattaa aaatcttcgg aatgacagag aacctattgg
    12001 tccattaatt acaatattta gtttagcacc aatttcaagt acatttggta taacagcaat
    12061 tggacttgga ataaaaagag cagtaattct taaaaaatca aagaaaaata ttttgaaatg
    12121 taatttctaa atatagaata aagcatacca caaaaaaaac actagttgtg gtagatttgt
    12181 tgattcaagt tttgtgcatt atatttaatt tgtaaataag tgttgattgg tgaaaaaata
    12241 cgcacaacaa acgccaattt tcagccgtta aactctcttc ttgattctaa tttttattat
    12301 caaaaattat caagttcaat ttcttttgtt agtctatttg attgcttatt ataaataata
    12361 attaaacctt agtaggaaca cattttttac aaaaaataaa attgtaaatt gcactgataa
    12421 aacaatatag tgtccacgca aattttaaca caccacgaag caatacaatt agagtgcggc
    12481 attaccttgc ctaaggtaga tattgcctat tctacatttg gagaaatgaa tgcagataaa
    12541 acaaatgtgg tttggatttg tcatgctttt tctgctaatt ccaatccaga agaatggtgg
    12601 ccaggcatgg taggtgaagg cctattttta gatcctaaaa aatattttat agtctgcgca
    12661 aatatgttag gttcttgcta tggaacgaca ggcccattgt ctgaaaatcc tgttactggt
    12721 aaaaaatatt ataaagattt tccgactgta acagtgcgcg acatggtgca aacgcttacc
    12781 atcttacgca aacatttagg catagaaaaa atattttttg catgtggttt ttctatgggt
    12841 ggccagcaat tattagagtt tgtcattcaa gaaccagata tttttgaaaa tattttatta
    12901 ggcgcaacca atatacagca ttcgccttgg ggcattgcat tcaacgaatc acagcgtatg
    12961 gccattgaag cagatactac atttggcgat gacagcgatg atgctggttt gaatggcatg
    13021 aaagcagcac gtagtattgg tttaatctcg tatagaaatt acaaagcata taatattaca
    13081 caagctgagc ctgataataa taaattggaa aattataaag cctgttctta tcagcgttat
    13141 caaggtgaga agctggcaaa acgctttaat gcctattctt atcacatctt gtctaaggca
    13201 atggacaacc ataatatcac acgcaataga ggcaatgcaa aagatgtatt gtcagccgtg
    13261 aaatgcaatg tacttgtgtt aggaattact tccgattatt tgtatccatt ggaagaacaa
    13321 caattgttgg cacgcttgat tccgcatagc atgtttgtag aaatagactc agattatggg
    13381 cacgatggtt ttttaatcga gaatgagcaa atgtctaaaa ttttaagcgc atttttgaaa
    13441 gtgtaattag ctttccattt cttttaaaaa ctttggccac aataattttt cggcactaga
    13501 aaaaaagaaa ccacgatgtc caaatttttt gatgtcataa tcagcagcat ataaagtttc
    13561 tatggatctg tctgcttgtg aatataaatc taaaataaag cggcaacttt tccgattggc
    13621 aattgcatcg tcagccaaat ggaatgcttt cacaggtgcc gtaattgtat ggtaatatga
    13681 tggtatcttt ttttcttcga aatagcttgt gaaaaaatca ggacgatagc accagctacg
    13741 caattctaaa attatctgtt taggaaaacc gccactaatg ccaaatgcag cattgttcat
    13801 atagccatat ttccaaatag aaaatggaac aattattttc caaagaaaaa agatggctat
    13861 tctcatagcc aaagaaagat tttttacata gccatttgcg caagctacta aatatatttt
    13921 ttcaaatgct gttgcatttt tagtatagcc tataaactgc ccgccagagc tatggccaac
    13981 agcatatttc tttaatgttg gatagttgtc gataataaat gccgtaacac tttctatatc
    14041 cttcaaccaa acagcatgtg tcacgtttga ttggtaatta tcggcatctg tatagtcgta
    14101 agtaatagtg acgtatccat tttttgtcat gtattcagca aatttccagt atattttttg
    14161 aggaatacag gtacctgtgt ttatttgcac tatgcctttg atgttgtcat tgggtgtgcg
    14221 aacagtgagc ataatttcat gaccagaagt agaagcaatt gcgatattgg ttgttgtcat
    14281 agcatttaaa aatactaaaa tgattttgta gtgcttaatt aaatattgaa atttctattt
    14341 ttatcagtgt tgcatttacc actttaattc agcatattta tttattaaat tcaagctttg
    14401 catttttgaa atagcatgta gttgtggtaa ttttaataga cacttttcaa tgacaagaaa
    14461 aacgataatt ttaataggtt tcattggact taaattcttg ctgcaatact ttctaataag
    14521 tagtgaatat gaactgcaaa gagatgggtt tttgcatctt gaccaagcca atcatttagc
    14581 ttggggttat ctttcagttc caccttttac atcttggata tcctacatta ttcaaatgtt
    14641 aggtaactca attttttggg taaaattttt cccagctttg tttggtgctt tgactatcta
    14701 tgtggtttgg aaagcaatag aggaattagg aggaaattta ttcgcattga ttttgggtgc
    14761 aaattgtgtt ttgttttcag cactattgcg attaaatacg ctgtatcaac ccaattcttt
    14821 tgatgtctta agttggacgg ttttttactt cattctacta aaatatatca aaacagatga
    14881 tgacaaatgg ctttatattg gagcgacagt atttgcactt ggctttttaa ataaatataa
    14941 tattgttttt ctattgattg gactttcgcc atctttattg cttacagaac atagaaaaat
    15001 atttttaaaa cctactttgt attttgctat tttattagga ttgttgctaa tcttaccaaa
    15061 tcttatatgg caatacaata acaattttcc agtctttaag cacttaaaac aattagcaca
    15121 aattcaatta gtaaatgtta atagatttga ttttttgaaa aatcaattgc tcttttttat
    15181 aggttcactc attgtcatct tttcatcatt ttatgccttc tttttttata aaccttatca
    15241 aaaatatcgg attttctttt tctcgataat ctttacatta acagcctttc tttactttaa
    15301 agcaaaagat tattatgcca ttggtattta tcctatttac attgcctttg gctctgtttt
    15361 tcttgcagat gtattaaaag gcgctcggaa aaaatttcta caaccaattg taattgtaat
    15421 tccgctgctt ttatttattc caatttataa tgttgcattt ccaaataaaa gtccagcgta
    15481 cataattcag cattcagaca actacaaaaa gttgggtatg ttgcgttggg aagatggtaa
    15541 agatcattca ttaccacagg actttgcaga tatgcttggt tggaaagaat tagcacagaa
    15601 agttgataga atttatttaa gtttagcaaa caaaaataat acgcttattc tttgcgataa
    15661 ttacggacag gcaggagcaa taaattttta tacgaaccaa aaattacgag cagtttcatt
    15721 taatgctgac tacatagatt ggtttgattt aactaaaaaa tatgaaaact taattagcgt
    15781 aaaagaagca gaagaaggaa gtgttgaatt tcaagaaaca gcgccatttt ttctacattc
    15841 atcgcttgca gactcaataa gtaatcaata tgccagagaa tttgggacga ggatatttgt
    15901 tttcaaaggt gcaaaaattg acattaggca aagaattaaa aatgaaattg atgaaaataa
    15961 aaactaaggc taagaaggct tggcatcagc catatagcca agctgaaaaa ctttaaacat
    16021 agatacatct tgattccaac aatagtacac gatttttata aattattcta tccagcgatt
    16081 tgtggtggct gcgatactac tttagcaaaa ggtgagcagc agctttgttt tcattgcaga
    16141 ttggcattgc cgtttacaca gtttgaaaaa atccagaata atcctgtcga acaattattt
    16201 ttcggacgag tgccaattga attcggcact gctttgcttt atttttcaaa agcagaaatg
    16261 gtgcaacaga ttatgcataa tataaaatat aacgataaaa aagatttagc tcaattgctt
    16321 ggaaaaatat taggtgaacg cttacaaaat attgcaagcc tacacgacgt tactgcaata
    16381 gtgcctgtgc cattgcacga acgaaaatta cgaatgcgtg gatataacca aagtgaatgt
    16441 ttagcagaag gtattaatga aattttgaaa gtggacatag tagtaaatgg aatacaacgc
    16501 ataaaacata cagattcgca aaccaataaa aacatgatag aacgctggga aaatgtaaaa
    16561 gatgcttttg aaataaaaca aattgaaaaa ctgaaaaata aacacatact tttgcttgat
    16621 gatgtgctaa cgactggtgc tacactagaa gcatgtgcgc ttacattaat tgaaaaagca
    16681 aattgcaaag taagtattgc tacattggcc tttgttgccg actaattatt tgatgaattg
    16741 attatctttg atacggatga aaagaacagc aataattttt gagaaattta tagcatacag
    16801 aatgctattg ctttttggtg ttttatttat tttttccttt acagcatgta aaaaagaaca
    16861 gctcaatact tcatcaggta tttctttttc tacagatact ttatctttcg atacactatt
    16921 ttcaacattg ggttcgacta ctcgattttt taccataaaa aatacgcata aacaagctat
    16981 aaaaatttcc aatatacaac tcgcaggcgg cataacttcg ccatatagaa ttaatgttga
    17041 tggcgatgct ggtacttcat ttaatgatat cgaaattcca gcaaaagata gtatttatgt
    17101 ttttgtagaa gtaacaatta atccaacggc agctaatttg ccatttatag tggaagattc
    17161 agtacagttt caaaccaatg gcattttaca gcaagtacaa ttaaatgcat acggtcaaaa
    17221 tgctcatttt tttaacggcg attctattga gaataacaca gtttggaacg acgatttgcc
    17281 atatgtaatt ctgaactact tgcaaataaa accgggtgct tcgctaagca ttcaagctgg
    17341 ctgcgatgtc tattttggaa gcaatgcagc attgctagta gaaggcgatt tgaatgtgaa
    17401 cggcacagac acatcgaaca tggttacatt ccgtggcgtg cgtttagata aagatattgc
    17461 cgacagaagc tatgacgatt ttccaggtca atatgcaggc gttttttttc tgagaaatag
    17521 caatggaaat attgagtacc tcaatatgcg taattcatta tatggaatta atgttggcaa
    17581 catcaaaaca tcagatgacc cagatgaaaa cttagcggca ctgcaagcaa tgagtattag
    17641 caatgcacca acattgacca ttaaaaattc aaaaatttat aaccatgctt tttatggctt
    17701 atttggcttt ttaggaaaaa tatatgcaga gaatacattg atatataatt gcggaaaaaa
    17761 tgtcgtcggc ttgtttgatg gtggcgatta tcaattttta aattgcacgt tttacaatag
    17821 aagttctgct tatatttcac atgctaaaga acctttattg tacatgaatg attattttaa
    17881 aattggcgat aatacgtatg ttattgcaga tgcggcaaca gcagtatttg aaaattgtat
    17941 cgtatcaggc acccttgaag aggaaattat aaaagacgat atcacacaaa atccattgcc
    18001 tatttctttg agctttgacc actgtgtggt gaaaacaaaa gagcctttga atgcaccaac
    18061 attccttaat tgtaaaaatg aaaatcctat ttttaaagat attttcaaaa atgatttcca
    18121 tctcaaatct acatcgccat gcgtaggctt tagtacttcc aattttccaa taattgacat
    18181 tgatggtttt gctagagcga acacgccaac agattgcgga gcatatattt ttcagtaatg
    18241 cacacatagt aataatttgc tattgaccac ctgttttttt tatgctaact tgttgatgtg
    18301 aaaattatat tcagcaagtt tttacataat atctattata gctttccgat acaattattc
    18361 atcttgcatt ggaataagca tttgtttgta ttgctgtttt ggataatttt atttctatcc
    18421 gtttcgcaat cttttggcgt aagctacggc attccacttt tgttccttga tcctgaatat
    18481 gtaggacacg ttaatttctt gagctttgtg attgttggtt tttcaatggg cggttttatc
    18541 atggcgtgga atatgtcttt ctatgtgttg aatagttatc gatttgagtt tttggcgtct
    18601 caagtaaatc cgtttgtgca gttttgttgg aataacttta ttattccatt ctcgtttgtc
    18661 attgtgtata tcgtgcagtt agtaaaattt caacacgctg aagcgctatt gccagcaggg
    18721 aaaattgtgg caaatgtaat cgcattactt tgtggaaatt tattgatgat tattgtttcc
    18781 agccttttct ttgttttgtt taatcatgat gtagaaacat ttttagctaa gctaagcgat
    18841 aaagcaaaaa catctttact cggtaaaaaa atacatttag ataaattgga attcggcacc
    18901 acctcagcag cacagtggca agtagagaaa tttattgcct ttccattttc tataaaaact
    18961 gttcgaaatg ccgattttta tgataaaaaa ttagtcgcaa aagtattatc gcaccaccat
    19021 agaaatgcga ttttgtttca atgtttagcc atttgtggtt tattgttatt aggtaaatta
    19081 atggacaatc catatttcag attgccagca agttcagcga tattcttgat tttttctgtg
    19141 cttgttgtgt ttttttcttt cttgtcattt tggtttcgca catggcgaac tgttgttgtc
    19201 attgtcttct tttttattct aaatattttg acgcaatacg atcttgttgt atataaacat
    19261 aaattgtacg gtctaaatta ttctgcccaa aaaattagct ataataatga agtgataaat
    19321 aatgcagtgc acaagccact cgtagatgct gatattattg ctactaaaaa aatattagaa
    19381 aattggaaga aaaaaatgca cgataaatat ggcgaaacaa aacctaaaat gctattcgtc
    19441 aatacggctg gcggtggttt aaaagctgca tattggactt tttatttatt gcaacaatta
    19501 gaaaataaaa cacaacaaaa attatttgac catactatgc tcatgacggg cgcatctggt
    19561 ggaatgattg gtgctgctta cttcagagaa ttgtatttac aacaagctac tcggccatct
    19621 gtaaattatt tagacacaac ttatttgtat aatattggac tggatttact gaacggaatg
    19681 gcaacatcta ttgccacaaa tgacattttt tatccttggc aatcctattc ttatggtggt
    19741 tataagtata aaaaagatag agcttatatg tttgataaaa agctcaatga gaatacaaat
    19801 tttgtgttgt cgaaattatt gctcgattat aaacagccag aaacaaaagc tcaaattcca
    19861 atgatgattt taagcgcgac catcataaat gaccaacgtt ttttattttt ctcgccccaa
    19921 tctatttctt atttaataaa accatacatt aaaaattcaa aaggctatgt cgatgatatg
    19981 tcaacagatg ctattgaatt tagacgtttc tttaaaacac aaggcgctga tagtattaat
    20041 tttattgatg ctttgcgcac aaatgccacc tatccataca ttatgcctgc cgtgtatttg
    20101 ccaaccacac cagagataaa agccatggat gcaggcatac gcgaaaactc tggattggct
    20161 gtatctacaa ggttttacag cgtatttaaa gattggatag atgcgaatac ttcaggtgtg
    20221 gtgtttctgt catttcgtgt tgataataaa ttacacgatt ttactgcaca aaaaaaacaa
    20281 acgtacttgt cagaattatt gtcgcccgta ggtagcattt ttaataattt tattctattg
    20341 caagattata attctgatgt gagtttagcc tacttagaaa atgcgagtag cactgatatt
    20401 aatgtgctca attttaatta tgaccaaacg agtaaacgca aaaaagcatc gatgagttgg
    20461 catttgacca ctgatgaaaa acgtgatata aaaagcgcat tttatcaaaa gaataatcaa
    20521 ttaatgctgg agaaattaaa cgaacttctt caataggaaa tgctatatca attccaataa
    20581 cacaatattt tcaatgtgaa aggtatgtgg aaacatatcg acaggtcggc aacgcgttac
    20641 tttgtatttt tctgaaaaca ataacacatc gcgtgcttga gttgaaggat tgcaactcac
    20701 atatacaatc tttttcggtg cagcttccag caatacattt accacttttt cgtgcaagcc
    20761 agcacgtggc ggatcaagca ctacaatgtc tggcgttgca tgtactgcaa taaattctgg
    20821 ttttaaaata tcttcgcaag tgcctacata aaaatcgcaa ttttcgattt ggtttaatgc
    20881 tgcattctct tttgcatcag caatagcatc aggtatttgc tcgatgccaa gcactttctt
    20941 gcagtcatcg gcaatatata aagcaatact tccagtgcca caatataaat catacacaaa
    21001 gtcgtttttg ctaaatccac caaattcttt tactaaatca tacagccttt tggcttgtct
    21061 gctattggtt tgaaaaaatg atttcggact aattttatat tttatatttc caagctgctc
    21121 gatgatatga tttttgttgt tgtagcaaat cacatcttga tcataaatcg tatcattctt
    21181 cttagtgtta atgatataat ttagagagct aatgtaagga aatgtatttt gtagataatc
    21241 catcatggca gtaatttcag gcattttgtt ttctgcaaaa cacacagtca ccatcatttc
    21301 atcaagcgtc gaacttctaa aaattaaatt gcgcatcaag ccttcgtgat gctttaaatt
    21361 gtaaaaagta tattgatgtt gaatagcaaa cgaatagaca gcattgcgaa tggtatttac
    21421 tttttcatct tgtagataac aggtgttaat atgcaaaatg gatgcgaaat ttcctgcggt
    21481 atggaagcct aatgcatttc tgttaaacga ctcaccacta tcgatttgct cacgcgtaag
    21541 ccacacgcga ttcgaaaatg taaactccaa tttattgcga taaaattctg ttgcctcaca
    21601 agccagaata ggttcgacgt tttccagctc aatttttcca attcttcgaa atgcatcgag
    21661 caccaactga tgcttcattt ctacttggta agcatagtca acatgctgcc acttacagcc
    21721 accacaagtg ccaaaatgtt cacaaaaaaa atctttacga tgtgcagatg cttctaccaa
    21781 tgtatcaatt tcggcaacac caaaatcctt tttctttttt gttactttag catgtataac
    21841 atcgcctgga atggctttat cgataaacca tacaaaatta tctactcgag caagcccttt
    21901 gccttcggca gcaattgctg ttactgctac attttcaata attggatatt ctactttctt
    21961 tttacgtcgc atatttctaa aaacttgtta aaattaatca aataaataat gactttgctg
    22021 tgtagatatt aaagtctgat aaaattcaat acatttgtta taaatccaat gatatgaaaa
    22081 aagctctact cacgattttc gctatttctt ctttcgccat aacaacagct ttctcgcaat
    22141 cagaaacatt gtttacagtt ggcgacacga aagtaaaaaa atcagagttt gaatatatct
    22201 acaagaaaaa taatttttcc aacaaagcag attttagcaa aaaatcactc gatgattatt
    22261 tgaatttata tgtcaatttt aggttgaagg tgaaagaagc tatggcgcaa ggattagaca
    22321 caggtagccg ttttaaagaa gagcttactg tgtatgaaaa gcaattgtta gaatcatacg
    22381 tagaaaaaga tgtattggat aaacttgtca aacaagaatt tgaacgctca aaaacagacg
    22441 tgcaattgag tcatattttc tttagtaata gcaatggtga tgaacaagca ttagaaaaag
    22501 ctaatgaagc ctatgctaaa ttaaaagcag tcgcagcatt tagtgaagtc gccaaaacat
    22561 cagatgataa atcgacagca tcgcaaggcg gaaaagtagg ctggatgaat agttatcaaa
    22621 tgacgtatcc gcaaatggaa gaaatagcat acagcctaaa aacgggcgct tatgctaaac
    22681 cagtaaaaac acaacttgga tatcatatca tacaattaga cgctacaaga ccagcacgcc
    22741 caaaaataaa agcagcaata ataaaacgct tcttgccaat tgctgatacg agcgccgccg
    22801 catttaaaca aacggaagat tcgatgaaaa tggcatatgc tgctttgaaa tctggagctg
    22861 cttttgaaaa aatagtagaa cgctttagcg aagatgatgc ttcgaaatcc aataaaggag
    22921 aactcgattg gtttggtatc aatacgtatg caaaaatatt tgaagaaaca gcctatgcat
    22981 tgcaagatgg tgagtattca ttgcctttta aaacaaaaac agcttggtat attgtaaaac
    23041 gtgtgcaaac tgcaaaacca ttaacaatag agacagcaac accaatttta aaaactaaat
    23101 taccaagctt gccgatttat caatatgaaa tggataagtt tattcaaaca ataagcaata
    23161 aattttcggt gcaagagttg acagaaaata tcaactcatt caaatcacga attacggttt
    23221 tagcaaatac ttctccattc aaatatcgcg atactgctag tacaaaacca ctattgaaaa
    23281 ttggcaattc gacatttaca gaaaatgatt tcggcaaaaa attccaagat atattttata
    23341 ctgtttatcc aaagccaggt gctgaaaaat ttgatgtgct ctataaaaat acactacaag
    23401 ctttcatcat tgattattat aaacaatttg taaaagaaaa taacgcagag tataaatcat
    23461 tgatggatga atataaaaat ggcattatga tttttgcatt gtcagaaaat aatatctgga
    23521 ataaggcatc atcggattct gtaggtttat tggcctatta caaggagcat caacaagatt
    23581 ttaatttgaa aaaacgagct tctgtaagaa caatttcagc agctaatagt aaacaaatca
    23641 atacaattta taagttgtta ctcgctaatc ctactatttc cgacgattct ctattgacag
    23701 taatgaaatc tattggactg acgagtgcgc aactgaaact cgccacagca gattccactc
    23761 aaaaaaaatg gaatatcaat aaagagagtt tgtctttgcc aaaatatgtc ggcaatagct
    23821 acacgatcac acaagtatat aacttacaag ccgccaaaac tcgccattta gatgaatgta
    23881 gaggttatgt cgtggcagca tatcaagaa
//
LOCUS       DAOQQV010000029        23329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_652_length_23329_cov_7.471556, whole genome shotgun sequence.
ACCESSION   DAOQQV010000029 DAOQQV010000000
VERSION     DAOQQV010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23329
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_652_length_23329_cov_7.471556"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PLL59_04805"
     CDS             complement(<1..1413)
                     /locus_tag="PLL59_04805"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HMY23157.1"
                     /translation="MKIGIAQLNYMIGDFNGNFEKMKQAIEQAIATNADLLLFSELCV
                     CGYPPRDFLEFNHFIKECENTIHQLATVAADKIAILVGAPSRNPSKDGKDLFNSAYFI
                     ENGKIKYIAHKTLLPTYDVFDEYRYFEPNKVFDVVDFKGKKLAITICEDIWDTGEENP
                     LYRINPVAELAKHKPDILLNLSASPFNYQQAKKRIDVIRKNATQFQLPVFYCNGVGAQ
                     TELIFDGGSVVMDAHGNVIDEMPYFETCVRFYDTENLLPSIEQDKNATKLIHDALVCG
                     IQNYFQKLGFKKAILGLSGGVDSALVLALAVRALGKENVLSVLMPSQFSTSHSVDDSM
                     KLLENLGSPYKIIPIKDVYDVYEKILQPHFENKPFDVTEENLQARIRGNYLMALSNKF
                     GYILLNTTNKSEAAVGYGTLYGDMCGGLAVLADVYKTQIYDVCHYINRDEEIIPNHIL
                     TKAPSAELRDGQKDSDSLPDY"
     gene            1491..2537
                     /locus_tag="PLL59_04810"
     CDS             1491..2537
                     /locus_tag="PLL59_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23158.1"
                     /translation="MKKIALLLMFVISCIGFQSCTCNRTNNLPKKEKAVEKSAVTVEI
                     IRFEKEFFACNPNKLDAEIPALENKYPTFSNVFFNQVLHLQDMQDKGMQYNYLQEFLT
                     NKYNKGLYDTVNAKLSNLDFLEKDLKIAFANYKSYFPEKPIPKVMTCISEFQIGAFTA
                     SDSIIGISLDMYLGAKYVYYADIFQQYTFMIPTFDKHFMSLDCANVLASNIIPPPSDK
                     STLLDKMLAKGKILYMMKALLPEKKDYDVIKYTDKQWQFCVENEVNIWTYFLNLKLLF
                     NTSQEQFKYVIESPTTYGMPKETPGRAGAWLGWQIINAYMKEHPNTTLKQLIAIKDGQ
                     QLLKESKYKPSFKN"
     gene            complement(2553..3314)
                     /locus_tag="PLL59_04815"
     CDS             complement(2553..3314)
                     /locus_tag="PLL59_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMY23159.1"
                     /translation="MKFTFLGTGTSQGVPVITCKCEVCQSSDKRNNRTRTSLLIQSDT
                     TTVVIDTGPDFRTQMLRENVQDLDAVLFTHGHKDHVAGLDDIRPFNYLLEKTIQVYAE
                     NSVQEILKREFSYAFVGHDYPGAPQIQLHTIDETPFLIGDIPFIPIRAIHKTLPVLGF
                     RIHDFTYITDANFIADNELEKIKGSKIVVLNALRKEPHYSHFSLPEALKIADEIQAEK
                     AYFTHISHHLGLHEQEENELPKNRYLAYDGLCINL"
     gene            complement(3372..3647)
                     /locus_tag="PLL59_04820"
     CDS             complement(3372..3647)
                     /locus_tag="PLL59_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23160.1"
                     /translation="MSFKYNAATLTTLESIFKVNHYVVRYEKGRFQSGYCVLQDKRVA
                     VVNKFFETEARINALIEILSQIEVDVTLLDDKQIKLYEQLAQQKLEI"
     gene            3797..4237
                     /locus_tag="PLL59_04825"
     CDS             3797..4237
                     /locus_tag="PLL59_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMY23161.1"
                     /translation="MLSKKTKYAINALVYLAKQPAKQPILISEIAEKERIPKKFLETI
                     LLDLRNAGILNSKKGKGGGYYLIKHPSEVNLAEIIRYFDGAIGLLPCVTHKYYEKCEE
                     CDDEVTCGIRHAFLAIRNETVEMLKKDTLESIIKNEKKLKSHVN"
     gene            4227..4886
                     /locus_tag="PLL59_04830"
     CDS             4227..4886
                     /locus_tag="PLL59_04830"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HMY23162.1"
                     /translation="MLTKHTLQFLEELSHNNTKSWFEQHRNRYTQAQSEIKSFIEIWL
                     NEFGKIDESIAHLDPKKCIFRIHRDVRFSANKNPYKTNLGAYLYKGDKQANYAGYYLH
                     IEPGNCFFAAGNYMPMPEQLAKIRQEIDYNLHDFQKILQQKQFKEIFGNISSENKLKR
                     APKGYDEDNKAIEFLKLKSFVVSTKLSDEEICAKSMQTSIIQLSKTVKPFVDFLNNAL
                     D"
     gene            4972..6204
                     /gene="serA"
                     /locus_tag="PLL59_04835"
     CDS             4972..6204
                     /gene="serA"
                     /locus_tag="PLL59_04835"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HMY23163.1"
                     /translation="MAKEVSFPKEKIKILLLEGLHQSAFNEFNKKGYTSIDSYKEAMT
                     EQELLDCIENYHIIGIRSKTNLTAAVLEKAKKLMTIGCFCIGTNQVDLKKACELGIPV
                     FNSPYSNTRSVAELIIGNAIMLLRGIPQKNKFMHDGNWQKTATRSYELRGKTIGIIGY
                     GHIGTQVSVLAESMGLKVIYFDIEPKLPLGNANVVDTLDELLQKSDIVTLHVPATPQT
                     KNMIGKKEFALMKDGVIFQNLSRGTVVDIEALADAVKSGKISGAAIDVFPTEPKQNGP
                     GFESALVGLYNIILTPHIGGSTQEAQENIGTEVAVKLTGFLDNGSTLGSMSVPEISLS
                     VQQDTRRVLHFHNNVPGVLSEINSTLSKHDVNILGQYLKTNESIGYVVLDVDGNADLE
                     MMEDLKKVKNTIKARILY"
     gene            complement(6209..7030)
                     /locus_tag="PLL59_04840"
     CDS             complement(6209..7030)
                     /locus_tag="PLL59_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMY23164.1"
                     /translation="MNTILVPTDFSAVAANALKAAKHLAKKSNAAIHLVHFYQLPLAD
                     YSNPDMLLPTEILEHHKHEANVALQNLVASLKEEGFDADASLKFGLLTEEIESLASSL
                     NSMMIVMGTTGASNIVNKLIGSNAANVLQHTEHPILLIPSNCHCDSFSNIVYLAELKE
                     DDTEVLSKVFAFAEMVNALHVRLLNVNTGFFFTPINEHLMIQLNRAFGLKRIKMDTVD
                     GADLVEGIEHYLENHHVNLLVMSTHKKSFLSRLFGKNDTIKMAQYTKVPLLVYHK"
     gene            7238..7822
                     /locus_tag="PLL59_04845"
     CDS             7238..7822
                     /locus_tag="PLL59_04845"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMY23165.1"
                     /translation="MINLILFGPPGSGKGTQAAKLAKAFNLYHISTGDLFRHEMQNET
                     PLGLEAKKYMNKGELVPDEVTIGMLSNKLDEQVGKVDGFIFDGFPRTQPQAEALDKLL
                     DLKNTAISLVLALEVGEDEITKRILDRAKQSENPRPDDLDESIIRNRFQVYLKQTAQV
                     ADHYKQADKFVTLDGIGSIDEIFDSLQAAVNDIK"
     gene            7895..8887
                     /gene="obgE"
                     /locus_tag="PLL59_04850"
     CDS             7895..8887
                     /gene="obgE"
                     /locus_tag="PLL59_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMY23166.1"
                     /translation="MEGSNFIDYVKIFIRSGNGGGGSVHFRREKYVPKGGPDGGDGGR
                     GGHIILRGNKQLWTLLHLKYKKHIHADNGAYGEGGLRSGKAGEDIIIEVPLGTVAKNG
                     ETFEKEVEILEDGQEVIWLKGGRGGQGNNHYKTAVNQTPRYAQPGEEGEEGWKILELK
                     VLADVGLVGFPNAGKSTLLSVLSAAKPEIADYPFTTLVPNLGVVPYRGDRSFIMADIP
                     GIIEGAHEGKGLGVRFLRHIERNSCLLFLIPADADDIKKQYKILEKELKLYNRELVNK
                     PRMIGISKSDLLDDELIEMLKKELPKKIPHVFFSSMTNKNIMELKDMLWKMMNS"
     gene            8966..9964
                     /locus_tag="PLL59_04855"
     CDS             8966..9964
                     /locus_tag="PLL59_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23167.1"
                     /translation="MKNHFYILLLCIICIACEKKQQTSFYFWKTSYQLSANEQKYLDS
                     LGVQKLYVRFFDVDIKNGKPLPIGEIKIQTQNTKQEIIPVVFITNETFAQIDQKNIEK
                     LATHIQKEIAFLFPKISSKLLKEIQFDCDWTSSTQVNYFSFLKIIQQNNPDLSISVTI
                     RLHQIKDKIKTGVPPVNKGVLMYYATSNPLTFDAKNSILDNALAKNYISTLADYPIAL
                     DLALPIYSWAIVQNQVGEKKLINGIRNADLRDTTLYQALASNFYFIKKDHYLKGNFLY
                     EDYTLKVEELSTQELNIAYKNFIKKNKKKELTTIFFQLDDATLSQYSIIQLKNILQ"
     gene            9961..12369
                     /locus_tag="PLL59_04860"
     CDS             9961..12369
                     /locus_tag="PLL59_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23168.1"
                     /translation="MKKYIIGIAISIVGIKASIACSWYSEDASYYNLFNQELIADKSL
                     SPFYLTYDVTFYGDSYEYDNESKDEINYNILEWKKYFDNQINEKELNYLVYQSSLNEL
                     TNIANTKSFNKVNDILKKQVYMTDKGMEAIQYLIFAKKCEPFATMTRENGDDEWSYSI
                     VRKTATKAQFMSIAKIGNGLYATTKNNDIKLRIAYQLVRLSHYAGFNDDAVRYFNTYV
                     EPLKQKSLFYYYALEQKGGALFNQKKYAEAGAAFASVFNNTTDRKIPCYASFRISNQM
                     DFNKSIAYCKTANEKAALYVLRGFNKFSNGLSEMKNIYAVAPKSAYLELMACRALMQL
                     EHKAFLVPNDYEYIRNSTFPTLDANSKVFLQKIILFTETLLKEKKASRTDFWQAYLAH
                     LYLLNGDYQKAKNTADKVISDDKDIIAQSNRTSFCAYIANLKTIDATAEQKINERYLS
                     KISNENEKNFVYEILAHNYIMQNNFAKAFLCHNDFTGLYGSLNINIITDLIALFEKEN
                     KSEFEQSLINNKIKSKEPLLELYDLKGTHYFKNDDLKNALVWYKKINKNIAFLKIHEY
                     QYDDEGKESVIEKDGDFNGYSNIDAGIFSNKVQTYFDDDIDNAFNDKTYQSFAFIENK
                     MNKRQLVEALIQLKKTAIGNDENAAKANFLLGNFYYNTSLWGFYRNFFYYEPGNYYLS
                     YVYTYNTIPNKLKESYNYNAGAGLISYNQPKKPYDYYLKAESLSSDKELKAKAVFQAS
                     KCELDLLFSAEDYNFYSNLKQLYNSTNRPMFKRLKTYYAQTAYFKEIQSNCSYFKYYL
                     QYRL"
     gene            complement(12341..13144)
                     /locus_tag="PLL59_04865"
     CDS             complement(12341..13144)
                     /locus_tag="PLL59_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23169.1"
                     /translation="MTFIEKINKIITQYIIRKASKNKAVDIVEVRTKQLGLLSVVNET
                     DLCIKNTLFIPYRLLSIETDLLNKDGIKVGKMSYDKPIRVKGNAEVVFTTVSEISIIT
                     SFFQALSNLLSQPIWMRSVGIAKVKVLWWTIDLPIDDVFEIQTSKIKIIPTETEEERQ
                     LRIASNEIKKQQRAAEKEEKKIKKEENRAERKENILKRRHKENYIPKEQREILSDTFE
                     DASVDENIELETSENTEETTQKTSETLANANDPAQKSKTQSTDDIADNT"
     gene            13268..14500
                     /locus_tag="PLL59_04870"
     CDS             13268..14500
                     /locus_tag="PLL59_04870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMY23170.1"
                     /translation="MEHLSTVIAIPIFLIFIIIEFFATRKKHPEYYRLNDTITNLNIG
                     VSHLLFKLLTTGLLLSAYVFVMEKFAVLHLHGITAYIVVLILFDFCFYWAHRFGHEIN
                     LFWGAHVVHHQSEEYNLSVALRQSWIHSFLAFIFFLPIAFLGVDVLTLGICASINSFY
                     QFWIHTKAVHRMPEWFEYIFNTPSHHRVHHGVNPKYIDKNHGGMFIIWDRLFGTFQAE
                     EEAPTYGITTPLKSWNPAWANIEYYVMLVKKAKPYSFADKLKLIFAKPGWQPNAFGGQ
                     LVIPAVDKSTPKYDANASSKNLNLYVLAQFVCIIIGLSAYLLYYEHLSIFYKIVFFSI
                     LMLSTVICSAILEQKNWVKYVEYIRLSIAAIALNTMYYINHNGWFYIVFIISCVLTAY
                     FLTWFTLNINKRFIFKIK"
     gene            complement(14518..14919)
                     /gene="mce"
                     /locus_tag="PLL59_04875"
     CDS             complement(14518..14919)
                     /gene="mce"
                     /locus_tag="PLL59_04875"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HMY23171.1"
                     /translation="MKKIDHIGIAVKDLASSNELFTKLFNKPPFHQEEVISEKLDVSF
                     FDLGDTKVELMQAHTPESKVYKYLEKKGEGIHHVCFEVENIEAEMERLKSEGFQPLTE
                     KPYIGALNKRVCFFHPKTTNGILIELCQKMK"
     gene            15051..16163
                     /locus_tag="PLL59_04880"
     CDS             15051..16163
                     /locus_tag="PLL59_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMY23172.1"
                     /translation="MIYLDYNATTPVDEQVLQTMLPYFSEKFGNAASSTHLLGAQAKD
                     AVEIARHHVANTIHCEAQEIIFTSGSTEAINHAIKGVFQLYQHKGKHIVTVKTEHKAV
                     IDTCKYLEKKGATITYLNVDKDGLIDLDELDKAISHETILVCVMYANNETGVVQDIKK
                     IAQITHQKNSIFFCDATQAIGKMPINVQADGIDLMCLSAHKMYGPKGIGALFVRRKNP
                     RVALEPLLHGGGHERGWRSGTLNVPAIVGLGKACDIIGENKFIEKLRDELEDALLAHY
                     QDKININGKHAIRLANTSNITFPFKAVDLIRQTKSLLSIATGSACTSAENTPSHVLTA
                     MGLSREQAEKSIRFSLGKYTTETEIQTVLTLIKHAF"
     gene            complement(16172..16618)
                     /locus_tag="PLL59_04885"
     CDS             complement(16172..16618)
                     /locus_tag="PLL59_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23173.1"
                     /translation="MRRLLFILFIVLSASALLLAKPPKMPKPKKGTDWVDPFKPKPLV
                     NIYGNWIWKETDCCGARRGISNPENTNDAITLELKSDNTFLETHAKTTALPRSGTVIM
                     FKENNIDMVQFNDERPAQYFLSNNGDTLTLSWKYMELQTEKYIRKK"
     gene            16690..17076
                     /locus_tag="PLL59_04890"
     CDS             16690..17076
                     /locus_tag="PLL59_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HMY23174.1"
                     /translation="MNEIQKIRIDKWLWSIRLYKTRTLATDACTAGKVKIDGETVKAS
                     YLLKVGQIININRQGEKWTLKSIKLIEKRVGAPIAITCYEDLTPPEEKDKLKFPSVFF
                     EVRDKGTGRPTKKDRRDIDKFKDIED"
     gene            17236..17436
                     /locus_tag="PLL59_04895"
     CDS             17236..17436
                     /locus_tag="PLL59_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HMY23175.1"
                     /translation="MFGMHGPELWFILAIVVLLFGGKKIPELMRGVGKGIREFNSAKN
                     NLQDEFEKGLKEDESKKLPESK"
     gene            17453..18868
                     /gene="gatA"
                     /locus_tag="PLL59_04900"
     CDS             17453..18868
                     /gene="gatA"
                     /locus_tag="PLL59_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HMY23176.1"
                     /translation="MEVYRSLKTLQEDLYNKKITATALTQQSISNILAQKELNAFVTV
                     FEESALASAKKIDEKLQSNQPLGKLFGLTIAIKDNIVYKNHIATGASKILSGFISPYS
                     ATVVEKLLAEDAIIIGSTNCDEFAMGGSNENSYYGAVKNPVDKERVSGGSSGGSAVAV
                     AANLCLAALGSDTGGSIRQPASFCGIVGLKPTYGRVSRYGLMAYGSSFDQIGTFTHTI
                     EDAALILEVIAGADAYDATCSPRAIPSYSNHISSNKKYKIAYYKNAIEHAGVDKEVSA
                     ATMQCIERLKSEGHQVDAIAFDELDYIVPAYYVLTTAEASSNLSRYDGVKFGYHAKEA
                     TDLESTYKLTRSQGFGKEVQRRIMSGSFVLSAGYYDAYYSKAQKVRRIVAEKTYNTLE
                     EYDFILCPTAPTPAYKIGEVQDSIASFLGDIFTVQANIAGIPGISLPLYTTSNGLPIG
                     IQLMAGRWQEEKLLAFANALM"
     gene            complement(18874..20547)
                     /locus_tag="PLL59_04905"
     CDS             complement(18874..20547)
                     /locus_tag="PLL59_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23177.1"
                     /translation="MKKNILIFIYLLVSIFIIGCLYGFPKIGIDDANIYFVYMKNFAD
                     GKGFVYNVGSESVEGFTSILWTLIGSFIFIFTNHIEICLLIINVVLLFFTLKIAIKII
                     ELFIKDEHLSANYITLFLGFLVVFPGFYDWTVLSLLETGLWTFELVLFTYLLIVPYLN
                     NNFSFQKSTIIISLLFPFFIITRPESILIGLVFIAIRVLQLFLEKISFRKILIHTIPM
                     CVVFIISLIALTKWRLYYFGYPFPNTYYVKMSDGFLNNLFEGAIYFGKYVSRTNPLII
                     AFFVLIFIWFIKMINEKDYTSNNKIIFSLVIISLVGIGIPFYTGGDHFRMSRFYQAFS
                     PIFHLCFLFLMLSFSHQNKLINKITSSRKKIFLSILILSFLPLKSLYVLFVNNKYSIA
                     IEFDIANENRKLGNNLNSFFDYQKKPSVGVIAAGGFAYTYSGYVNDVLGLNNIEVAHT
                     INDRPKYILKGHRAFNKTVFLKQKPDLFLFEFVADTSKFIPFTKRDYIVNGFGGKVIR
                     HIYNDTDFKKQYSPVFITNKKAKQTLFAYASKQFLSTLDTTLYVYKNVK"
     gene            20791..22455
                     /locus_tag="PLL59_04910"
     CDS             20791..22455
                     /locus_tag="PLL59_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMY23178.1"
                     /translation="MSIRLYFTIYILFFFFGFNQELKAQSPPPSQYKDAPSELYIPTE
                     EQLFKDDPDPVVLSAQIAVRLKKIQQIIPLDYNDHVQKYISYNTHEKRKEHIAKMLAR
                     AKKFFPIFEPILAKYGIPDEMKYLAVVESALNPFALSRVGASGPWQFMYSTALRYELN
                     INKRVDERRDPYLACEAAAKYLSEMYALYGNWQLAIASYNCGAGNVNKAIKKAGGSLN
                     FWNIRGFLPAETRAYVPSFIAVVYAMKYAGKYGISALPLNRENTQRITIRQKISVSDL
                     ESSLSIPRQDIIDENPSLLTTTIPADFILNLPVSKINAYYQFQDSLQQKALCELEEKQ
                     LVVPMVIKPTSSDAEISASKKSNVSTKEEWKNIPTAEKAASNVSIDKPLGKEDDVNYK
                     TMAYTVKTGDNLGKIAAWFHCTVKELRAWNKLQDNNIAVKDELLLYIHKNDYKKFVRF
                     NYLSASIKTDLSAKITTQEEKNNTLQQEKIEANTPLIDKINPIKILSKKDCYKKHTVK
                     SGESLYSIAQKYDDVSVQDLLLWNGFKKTPILHKGDIIKVRKIACK"
     gene            22578..23174
                     /gene="ruvA"
                     /locus_tag="PLL59_04915"
     CDS             22578..23174
                     /gene="ruvA"
                     /locus_tag="PLL59_04915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HMY23179.1"
                     /translation="MFAYISGKITYKTPTHIYLDCNGIAYYINISLNTYAAIEKQESV
                     KLYTHLVVREDAHVLYGFYTEEEKNLFLHLISVSGVGPNTARLVLSSMSTQEFQKAII
                     QADVRLIQSIKGIGPKSAQRLILELKDKLKKDATAEGMDNIAASSSNTLIKEEALAAL
                     NMLGFQKAQAEKAVQKSMQENTQIQSVEELIKLSLKNL"
BASE COUNT         8118 a         4001 c         3920 g         7290 t
ORIGIN      
        1 ataatcaggc aaagaatccg aatctttttg tccatcgcgc aactcggcac tcggcgcttt
       61 tgtcaaaatg tgattgggaa taatttcttc gtctctattg atgtaatggc aaacatcata
      121 aatctgtgtt ttatacacat cagccagtac tgccaagcct ccacacatat cgccatatag
      181 cgtgccatag ccaactgctg cctcgctctt gttcgtggta tttaataaaa tatagccaaa
      241 tttattactc aatgccatta aataattgcc tctgatgcgt gcttgcaaat tttcttctgt
      301 tacatcgaaa ggtttatttt caaaatgtgg ctgcaatatt ttttcataca catcatagac
      361 atctttaatc ggaataatct tgtatggaga acctagattt tccaataatt tcatgctgtc
      421 atctacagaa tgggaagtgg aaaattgtga aggcattaac acgcttagta cattttcttt
      481 accgagcgca cgcactgcta atgctagcac taaagccgaa tcgacaccac cactcaagcc
      541 aagaattgct tttttaaatc ctaatttttg gaaatagttt tgaatgccac aaaccagtgc
      601 atcatggatt aattttgttg cattcttatc ttgttcaatt gatggcaaca aattttctgt
      661 atcgtaaaat cgcacacatg tttcgaaata aggcatttca tcaatcacat tgccatgtgc
      721 atccatcacc acggagccgc catcaaaaat aagttcggtt tgtgcaccaa ctccattgca
      781 ataaaaaact ggcaattgaa actgagtcgc gtttttacga atgacatcaa tgcgcttttt
      841 tgcttgctga taattaaatg gtgatgccga taaattcaat aaaatatctg gtttgtgctt
      901 tgccaattcc gctacaggat ttattcgata taaaggattt tcttcgcctg tgtcccaaat
      961 atcttcacaa atggtaatgg ctaatttttt gcctttaaaa tcaactacat caaatacttt
     1021 attaggttcg aagtaacgat attcatcaaa aacatcatac gttggcaata aggttttatg
     1081 tgcaatgtat tttatttttc cgttttcaat aaaatatgct gaattaaata aatctttacc
     1141 gtctttgctt ggatttcttg atggtgcgcc gactagaatc gcaattttat ctgccgcaac
     1201 tgttgccagc tgatgaatcg tattttcgca ctctttgata aaatgattaa actccaaaaa
     1261 atctcttggc ggatagccac aaacacacaa ttcgctaaat aacaataagt cggcatttgt
     1321 agcaattgcc tgttcaatcg cctgtttcat tttttcaaaa ttgccattaa aatcgccaat
     1381 catataattg agctgtgcta ttcctatttt catgtcataa aagtaacaag ttatgcgtga
     1441 ttacttgcgt atttttatct tatttttgca agataaattt ttaatataaa atgaaaaaaa
     1501 ttgctctact gttgatgttt gtaataagct gcattggttt ccaatcttgc acttgtaatc
     1561 gtacgaataa tttgcctaag aaagagaaag cagtagaaaa atcagctgtt actgttgaaa
     1621 ttattcgatt tgaaaaagaa ttttttgctt gcaatcccaa caaattagat gctgaaattc
     1681 ctgcattaga aaataaatat ccgacattca gcaatgtatt tttcaatcaa gtattacatc
     1741 tccaagacat gcaagacaaa ggcatgcaat ataattactt acaagaattt ttgacaaaca
     1801 aatacaacaa aggtttgtac gatacggtaa atgccaagct aagcaatctt gattttttag
     1861 aaaaagattt gaaaattgct tttgcaaatt ataaaagtta ttttcccgaa aagccaattc
     1921 caaaagtaat gacttgtatt tcggaatttc agattggtgc atttaccgca tctgattcta
     1981 taattggcat ttctttagat atgtatttag gtgctaaata tgtttactat gctgatattt
     2041 ttcagcaata caccttcatg attcctacat tcgacaagca ttttatgtca ttagattgtg
     2101 caaatgtatt ggcttcaaac attattccgc caccgtcaga caaatcgact ttgttggata
     2161 aaatgctggc aaaaggaaaa atattataca tgatgaaagc attattgcca gaaaaaaaag
     2221 attatgatgt tattaaatac acagacaagc aatggcaatt ttgtgtagag aatgaagtaa
     2281 atatttggac ctattttttg aatttaaaat tattgtttaa cacttcgcaa gagcaattca
     2341 aatatgtgat tgaatcacca acaacgtatg gcatgcctaa ggaaacacct ggtagagctg
     2401 gtgcttggct cggttggcaa attattaatg catacatgaa agaacatccg aacacgacat
     2461 taaaacagtt gattgccatt aaagatggtc agcaactttt aaaagaatct aaatacaaac
     2521 cttcgtttaa aaattagata tcaatcaaat cattatagat ttatgcataa gccatcgtag
     2581 gcgagatatc gattttttgg cagttcattt tcttcttgtt catgcagtcc taaatggtgc
     2641 gaaatatgtg taaaataagc cttttcagct tgtatctcat ctgcgatttt taaggcttcg
     2701 ggcaaactaa aatgagaata atgtggctct ttacgcaatg catttaacac aactatttta
     2761 cttcctttta ttttttctaa ttcattgtct gcaataaaat tagcatcggt tatgtaagta
     2821 aaatcatgga ttcgaaatcc taaaacaggt aatgttttat gtatggcgcg aataggaata
     2881 aatggaatgt cgccaatcaa aaatggtgtt tcatctattg tgtgtaattg aatttgtggt
     2941 gcgcctggat aatcatgtcc gacaaatgca tacgaaaact ctcgtttcaa aatttcctgc
     3001 acgctatttt ccgcatacac ttgtatcgtt ttttccagaa ggtagttaaa cggtcttata
     3061 tcgtctaaac ctgcaacatg gtctttatgt ccatgtgtaa acaaaaccgc gtctaaatct
     3121 tgtacgtttt cgcgtaacat ttgagttctg aaatcaggac ccgtatctat gacaactgtt
     3181 gtcgtatcgg attgaatcag caatgaggtg cgtgttcgat tattgcgttt atctgaagac
     3241 tgacacactt cgcacttaca cgtaataacg ggtacgcctt gtgatgtgcc tgtgcctaaa
     3301 aacgtaaact tcattattga tgtacttatt gaacctttgc gtacactgat cagcgtacga
     3361 atgggcaaga attagatttc taatttttgt tgtgctaatt gctcatagag ctttatttgc
     3421 ttatcgtcga gcaatgtaac atctacttct atttgtgata aaatttctat aagcgcattg
     3481 atgcgtgctt ctgtttcgaa aaatttattg acaacagcta ctcgcttatc ttgcagtaca
     3541 caatagcctg attgaaatct tcctttctcg tatcgaacca catagtggtt tactttgaaa
     3601 atagattcta aggtcgttaa tgtagcagca ttatacttaa aactcatgaa acaaatgtaa
     3661 taagtaagac gcaatatcac gcatgatttt acacacaggc tcttaaaatg tgaaaattat
     3721 tttctctatt taatccatag acttttaaaa ttgagtatat ttaagcgatt tatcatattc
     3781 aaataattag taagacgtgc tatcaaagaa aacaaagtat gccattaatg ccttggtgta
     3841 tcttgccaaa cagcctgcaa aacaacctat attaattagt gaaatagctg aaaaggaacg
     3901 aattccgaaa aagtttttgg agacgatttt gttggatttg cgcaatgcgg gcattctcaa
     3961 ttctaaaaaa ggaaaaggtg gtggttacta tttaattaaa catccatctg aagtaaactt
     4021 ggcagaaatt attagatatt ttgatggtgc cattggcttg ctaccttgcg ttacgcataa
     4081 atattatgaa aaatgtgaag aatgcgatga tgaagtgact tgtggtattc gtcatgcttt
     4141 tttagccatt agaaatgaaa ctgtagaaat gctaaaaaaa gacaccttgg aaagcatcat
     4201 caagaacgaa aaaaaactaa aatcgcatgt taactaaaca caccttgcaa tttttagaag
     4261 aattaagtca taacaacaca aaaagctggt ttgaacagca tagaaatcgc tacacgcaag
     4321 ctcaatctga aataaaatct ttcattgaaa tttggttgaa cgaatttgga aaaatagatg
     4381 aaagtattgc acatcttgat cctaaaaaat gcattttcag gatacatcga gatgttcgat
     4441 tttctgccaa taaaaatcca tataaaacaa atttaggtgc ctatttatac aaaggcgata
     4501 aacaggctaa ttatgcagga tattacttac atatcgagcc tggaaattgc ttttttgctg
     4561 ctggaaatta catgccaatg ccagaacaat tggctaaaat tcggcaagaa atcgattata
     4621 atttacatga ctttcaaaaa atactacagc aaaagcagtt caaagaaatt tttggcaata
     4681 tctcttcaga aaataaatta aagcgagctc ctaaaggata tgacgaagac aacaaggcta
     4741 tcgaatttct aaaacttaaa agttttgtgg tgagcactaa gctaagcgat gaagaaatat
     4801 gcgctaaatc aatgcaaaca agcatcattc agctatcaaa aacggtaaaa cctttcgtcg
     4861 atttcttaaa taatgcctta gattaaagat aattttatca aatttttaaa tactttacct
     4921 taggcattcg cttttcgttt atctattaca caatgattac atttgataac tatggcaaaa
     4981 gaagtatcat ttcctaaaga aaaaatcaag attttattat tagaaggctt acatcaatct
     5041 gctttcaacg agtttaataa aaaaggctat accagcattg atagctataa agaagcaatg
     5101 acagaacaag aattgttaga ttgcatcgag aattatcaca tcataggcat tcgttccaaa
     5161 acaaacttaa ctgctgccgt tttagaaaaa gcaaaaaagc taatgacaat cggctgtttt
     5221 tgtattggca caaatcaagt ggacttaaaa aaagcttgtg aattaggtat tcctgtgttt
     5281 aactcaccat actcgaatac gcgttctgtt gcggaattaa ttattggaaa cgctatcatg
     5341 ttgttgcgtg gcattccaca aaaaaacaag ttcatgcacg atggcaattg gcaaaaaaca
     5401 gctaccagaa gttatgaatt acgtggcaaa accattggta tcattggcta tggtcacatc
     5461 ggcacacaag tttctgtatt agcagaaagc atgggcttga aagtaattta ttttgacata
     5521 gagcctaagc ttcctttagg aaatgccaat gtagtagata ctttagacga gcttttacaa
     5581 aagtcagaca ttgttacatt acatgttcca gcaacgccac agactaaaaa tatgattggc
     5641 aaaaaagaat ttgcgttaat gaaagatggc gttattttcc aaaacttatc acgcggaacg
     5701 gtagtagata ttgaggcttt ggctgatgcg gtgaaaagcg gtaaaattag tggtgctgct
     5761 attgacgtat ttcctacaga gccaaaacaa aatggaccag gttttgaatc tgcattagtt
     5821 ggtttataca atatcatttt aacgccacat attggtggca gcacgcaaga agcacaagaa
     5881 aatattggca cagaggtagc tgtaaaactt accggatttt tagataatgg aagcacattg
     5941 ggttcaatgt cagtaccaga aataagctta tcagtgcagc aagatacacg acgtgtattg
     6001 catttccata ataatgtacc tggtgtactt tctgaaatca actcaacgct ttcaaaacac
     6061 gatgtcaata tcttaggaca atatctaaaa accaatgaaa gcatcggtta tgttgtgtta
     6121 gacgttgatg gcaatgctga tttagaaatg atggaagatt tgaaaaaagt aaaaaacacc
     6181 atcaaagctc gtatcttata ctaagctatt acttatgata tactaaaagt ggcactttcg
     6241 tgtattgtgc catttttatc gtatcatttt ttccaaacag tcggctgaga aatgattttt
     6301 tatgtgtgga catcaccaat aaattcacgt ggtgattttc taaataatgc tcgattcctt
     6361 ctaccaaatc tgcaccatca accgtatcca ttttaatacg ttttaaacca aaagcacgat
     6421 taagctgtat catcagatgt tcgttgatag gcgtgaaaaa aaatccggta ttcacattca
     6481 acaaacgcac atgcaaagca ttgaccattt ctgcaaatgc aaatactttc gacagcactt
     6541 ccgtatcgtc ttctttcagc tctgctaaat acacaatatt actgaacgaa tcgcaatgac
     6601 aattagatgg aattaataaa attggatgct ccgtatgttg caacacattt gctgcattgc
     6661 taccaattag cttattaacg atattgcttg cgccagttgt tcccattact atcatcatcg
     6721 aatttaatga gctagccaat gattcgattt cttcggtaag taaaccaaat ttaagcgacg
     6781 catcggcatc aaaaccttcc tcttttaaag aagccactaa gttttgtaag gctacatttg
     6841 cttcgtgttt gtgatgttct aatatttctg tcggcaataa catatctggg ttagaataat
     6901 cagccagtgg caattggtag aaatgcacta aatgaatagc agcatttgac tttttggcta
     6961 agtgttttgc agcttttaat gcatttgcag ctactgcaga aaaatcagtg ggaacgagta
     7021 ttgtattcat agaattactt tttattaaag ttaatagtaa tcacaacaca tagccatgat
     7081 tattatcata tttaagaaat gaaataacaa aattgctatt tccattcata tttattaaaa
     7141 caacattact aaaaagtaat caatcgctat aaagtctcaa atgcaaaatc tcaaatgcaa
     7201 aatacgttgt atctttgtcg cttaacattt aataaaaatg ataaatctaa tattgtttgg
     7261 tcctccagga agtggaaaag gaacgcaagc agctaaattg gcaaaagcat ttaacctata
     7321 tcacatttcg acaggtgact tatttcgcca cgaaatgcaa aacgaaacac cacttggctt
     7381 agaggctaag aaatacatga acaaaggcga gctagtgcct gatgaagtca ccatcggcat
     7441 gttgtctaac aaattagatg agcaagtggg aaaagtagat ggctttatct ttgacggatt
     7501 tccacgcaca cagccacaag cggaagcatt agacaaatta ttagatttaa aaaacacagc
     7561 gatttcatta gtattggcat tagaagtagg cgaagatgaa attactaagc gcattttaga
     7621 cagagcaaaa caatctgaaa atcctcgtcc tgacgactta gatgagtcaa ttatcagaaa
     7681 tcgattccaa gtgtatttaa aacaaactgc acaagtcgca gaccactata aacaagcaga
     7741 taagtttgta accttagatg gcattggctc tatcgacgaa atttttgaca gcttacaagc
     7801 agctgttaat gacataaaat aacaacagaa gatatttaga gcttcaagtc ttaattggct
     7861 tgaagctctt tttatttcct atctttgcaa caaaatggaa ggttcaaatt ttattgacta
     7921 cgtaaaaata ttcatacgct ctggcaatgg tggcggtggc tctgtgcatt tccgaaggga
     7981 aaaatatgta cccaaaggtg gcccagatgg tggcgacggc ggtcgtggtg gacatattat
     8041 tttacgtggc aataagcaac tttggacctt attgcatcta aaatataaaa aacacattca
     8101 tgcagacaat ggtgcttatg gcgaaggtgg tctgcgttct ggaaaagctg gcgaagacat
     8161 catcattgaa gtgcctttag gtactgtagc aaaaaatggt gagactttcg aaaaagaagt
     8221 ggaaatttta gaagatggtc aagaagtaat atggctgaaa ggtggtcgtg gcggacaagg
     8281 aaataatcac tacaaaactg cggtgaacca aacgccacgc tatgcacagc ctggcgaaga
     8341 aggtgaagaa ggctggaaaa tattggaatt aaaagttttg gcagatgtgg gtttagtagg
     8401 ctttccaaat gctggtaaat cgacattgct ttccgtattg agtgctgcga agccagaaat
     8461 tgccgattat ccttttacca ctttagtgcc aaatttaggc gtggtgcctt atcgtggcga
     8521 ccgctctttt atcatggctg atataccagg aattattgaa ggcgcacatg aaggcaaagg
     8581 tttaggcgtt cgctttttac gacatatcga gcgcaattct tgtttattgt ttttaattcc
     8641 tgccgatgcc gatgacataa aaaaacaata taaaatttta gagaaagaac tcaagttata
     8701 caaccgtgaa ttggtgaaca aaccaagaat gataggcatt tccaagtcag acctattaga
     8761 cgacgagctg atagaaatgc taaaaaaaga actgcctaaa aaaataccac atgtgttttt
     8821 ttcttccatg accaacaaga atattatgga gctaaaagac atgctgtgga aaatgatgaa
     8881 ttcgtaattc ttatttacta aaaaaatacc tgctaatact taatgtatgg gaacttcgtt
     8941 ttacttaatt tagttatgta aaacaatgaa aaatcacttt tatattttgc tcttatgtat
     9001 catttgtatt gcttgtgaaa aaaaacagca aacttccttt tatttttgga agaccagcta
     9061 tcaactttca gcaaatgagc aaaaatattt agacagctta ggtgttcaaa aattatatgt
     9121 tcgttttttt gatgtggaca taaaaaatgg aaaacctttg cctattggcg aaatcaaaat
     9181 acaaacacaa aatacaaagc aggaaattat tcctgttgta tttatcacaa acgaaacatt
     9241 tgcccaaata gaccaaaaaa acatcgagaa attagcgacg catattcaaa aagaaatagc
     9301 atttttattt ccaaaaatat caagtaaatt actgaaagaa atacaatttg attgtgattg
     9361 gaccagcagc acacaagtta attatttttc atttttaaaa atcatacaac aaaacaatcc
     9421 tgatttatct atatctgtca ctatacgact acaccaaata aaagataaaa taaaaacagg
     9481 cgttcctcca gtaaataaag gtgttttgat gtattatgct acttcaaatc cattaacatt
     9541 tgatgctaaa aattccattt tagacaatgc acttgcaaaa aactacatca gcacattggc
     9601 tgattatcct attgctttag atttggcttt gcctatttac agttgggcaa ttgtacaaaa
     9661 ccaagtaggc gaaaaaaaat taatcaatgg tataagaaat gcagacttgc gagacactac
     9721 tttatatcaa gcattggcat caaattttta tttcataaaa aaagatcatt acctaaaagg
     9781 aaattttcta tatgaagatt atactttgaa ggtagaagaa ttgagcacac aggaattaaa
     9841 tatagcgtat aaaaatttta taaaaaaaaa taaaaaaaag gaactaacga ctattttttt
     9901 tcagttagat gatgcaactt tatcacagta ttcaattata caattaaaaa acatattaca
     9961 atgaaaaaat acattattgg catcgccatt tcaatagttg gtatcaaggc aagcatagca
    10021 tgtagttggt attcagaaga tgcttcgtat tacaacttgt ttaatcaaga actcattgcc
    10081 gacaaatcat tatctccatt ttacttaact tatgatgtta ctttttatgg cgatagttat
    10141 gaatatgaca atgaaagcaa agatgaaatt aattacaata tacttgaatg gaaaaaatat
    10201 ttcgacaatc agataaatga gaaagagttg aattatttgg tatatcaatc atcgttgaat
    10261 gaactgacga atattgcaaa tacaaaatca tttaacaaag tcaatgacat tttaaaaaag
    10321 caagtgtata tgacagataa aggcatggaa gctattcaat atttaatatt tgccaaaaaa
    10381 tgtgagcctt ttgcaacaat gacacgtgaa aatggcgatg atgaatggag ctatagtatc
    10441 gtacgaaaaa cggcaacgaa agcacaattt atgtcgattg caaaaattgg caatggcctt
    10501 tatgccacaa caaaaaacaa tgatatcaag ttgcgcattg catatcaact cgtacgctta
    10561 tcgcactatg ctggctttaa cgacgatgcc gtgcgttatt tcaacacgta tgttgaacca
    10621 ctgaaacaaa aatcattatt ctattattat gcattagaac aaaaaggcgg tgcattgttt
    10681 aatcaaaaga aatatgcaga agctggtgct gcttttgcga gcgtttttaa caacactacg
    10741 gatagaaaaa taccttgcta cgcgagtttt agaatttcga atcaaatgga ctttaacaag
    10801 tcgattgcat attgcaaaac tgctaacgaa aaggcagcgt tgtatgtgct gcgcggcttt
    10861 aataaatttt caaatggctt gtctgaaatg aaaaatattt atgcagttgc gccaaaaagt
    10921 gcctacttag aattgatggc atgtcgcgct ttaatgcaat tagaacataa agctttttta
    10981 gtgccaaatg attatgagta tatcagaaat tcaacgtttc caacactcga cgccaatagt
    11041 aaagtatttt tacaaaaaat aattttattt acagaaacct tactgaaaga aaagaaagca
    11101 agtcgaacag atttttggca agcgtattta gcgcatttat atttactcaa cggcgattac
    11161 cagaaagcga aaaacactgc agacaaagta atttcagacg acaaagacat cattgcacaa
    11221 agcaacagaa cctctttttg cgcatacatc gctaatttaa aaactattga tgccacagct
    11281 gaacaaaaaa taaatgagcg ttatcttagt aaaatcagca atgaaaatga gaagaatttt
    11341 gtgtatgaaa ttttagcaca caactatatc atgcaaaata attttgcaaa agcattttta
    11401 tgtcacaatg atttcactgg tttatatggc tccttaaata tcaacatcat cactgatttg
    11461 attgcattat ttgagaaaga aaacaaaagt gaatttgaac aatcgctaat caataacaag
    11521 ataaaaagca aagagccatt attagaatta tatgatttga aaggcacaca ttatttcaaa
    11581 aatgatgact taaaaaatgc tttagtttgg tataaaaaaa taaataaaaa tatagcattt
    11641 ttaaaaatcc acgaatatca atacgatgac gaagggaagg aaagcgtcat cgaaaaagac
    11701 ggcgatttta atggctattc caatattgat gctggtatat ttagcaacaa agtacaaacc
    11761 tattttgacg acgatattga taatgctttt aatgacaaaa cttatcaatc ttttgcattt
    11821 atagaaaaca agatgaacaa acgtcaattg gtagaagcct tgatacaatt gaaaaaaaca
    11881 gcaattggca acgacgaaaa tgcagctaaa gccaactttt tattaggcaa tttttattac
    11941 aatacttcac tttggggttt ttatagaaat ttcttttatt acgagccagg caattattat
    12001 ttgtcgtatg tgtacacgta caacacgatt ccaaataagc taaaagaatc ctataattac
    12061 aatgctggtg caggtttaat tagctacaat caacctaaaa aaccatatga ttactatctt
    12121 aaagcggaaa gcctatcatc tgataaagaa ctgaaagcta aggctgtatt tcaagcttca
    12181 aaatgtgaat tagatttgct tttctctgct gaagattata atttttatag caatctcaaa
    12241 caattgtaca attctaccaa tcgtccaatg tttaaacgtt taaagactta ttatgcacaa
    12301 actgcatatt tcaaagaaat acaaagcaat tgtagttact ttaagtatta tctgcaatat
    12361 cgtctgtaga ttgtgttttg cttttttgcg ctggatcatt agcatttgct aatgtttcag
    12421 acgttttttg tgttgtttct tccgtgtttt cagatgtttc caattcgatg ttttcatcaa
    12481 cagaagcatc ttcaaaagta tcgcttagaa tttcgcgttg ttctttcgga atataatttt
    12541 ctttgtgtct tctttttaaa atattctctt ttcgctcagc gcgattttct tcctttttga
    12601 tttttttctc ttccttttca gctgcacgct gttgcttttt tatttcatta gatgcaatac
    12661 gcaactgtct ttcctcttct gtttcggttg gaataatttt aatttttgag gtttgaattt
    12721 caaatacgtc atcaatcggc aaatcgatag tccaccatag cactttcacc ttggcaatgc
    12781 ctacgcttcg catccaaatt ggctgtgata atagattcga tagcgcttga aaaaatgaag
    12841 tgataatact aatttcagaa accgttgtaa atactacttc agcgtttcct ttcacacgta
    12901 ttggtttatc gtagctcatc ttaccaactt taattccatc tttattaagc aaatctgttt
    12961 cgatagacag taatcgatat ggtataaaaa gtgtgttttt tatacataaa tccgtttcat
    13021 ttacaaccga taataaacct aattgcttcg tacgcacttc aacgatatca acagctttat
    13081 ttttcgatgc ttttcggatt atatattgag taataatctt gtttattttt tctatgaatg
    13141 tcatattcta ttggtaaagt cttgttaaaa taaatattga tacttgtatt cttagtaaac
    13201 aaacattaac tttatttgtg ctgcaatatt aataatatta aagctacaat aacttaaatt
    13261 tttaaaaatg gaacacctta gcaccgttat tgcaattcct atttttttga tattcatcat
    13321 catcgaattt tttgccacac gcaagaaaca tccagaatac tatagactca atgacaccat
    13381 caccaatttg aatattggcg ttagccatct attgtttaag ctccttacaa caggactact
    13441 attaagtgct tatgtttttg tgatggaaaa atttgcagtg ctacatctac atggaattac
    13501 agcatatatt gttgtgctca tcttatttga tttctgtttt tattgggcgc atcgttttgg
    13561 tcatgaaatc aatttatttt ggggagcaca tgtggtacat catcaaagtg aagaatataa
    13621 tttaagtgtt gcgcttcgcc aatcgtggat acatagtttt ttagccttta tttttttctt
    13681 accaattgca tttctaggtg tcgatgtttt gaccttaggt atttgcgcca gtatcaattc
    13741 cttttatcaa ttttggatac acacaaaagc agttcaccgc atgccggaat ggtttgaata
    13801 tattttcaac actccgtcac atcatcgtgt gcatcatggt gtaaatccaa aatacatcga
    13861 taaaaatcac ggtggcatgt ttatcatttg ggacagacta tttggcacct ttcaagcaga
    13921 agaagaagcg ccaacgtatg gtatcactac accgctaaaa agttggaatc cagcatgggc
    13981 aaatatcgaa tattatgtga tgttggtgaa gaaagcgaaa ccatattcat tcgcagataa
    14041 attgaaactt attttcgcaa aacctggctg gcaaccgaat gcttttggtg gacaattagt
    14101 cattccagca gtcgataaat caacaccgaa atacgatgca aatgcgagca gtaaaaattt
    14161 gaatttatat gtattagctc agtttgtttg catcattatt ggtttgtctg cttatttact
    14221 ttattacgag catttgagta tcttttacaa gatagttttc ttcagtattt taatgctttc
    14281 tacagtgatt tgtagtgcca ttttagaaca aaaaaactgg gtaaaatatg tggaatatat
    14341 tcgcttgtca atagcagcta tcgccttaaa tactatgtat tacatcaatc acaatggttg
    14401 gttttacatt gtatttatta tttcatgtgt gctaacagct tattttctta cctggtttac
    14461 attaaatatc aacaagcgtt ttattttcaa aataaaatag actacgctta aaatagctta
    14521 tttcattttc tgacacaatt caatcaaaat gccatttgtt gtctttggat gaaaaaaaca
    14581 cacccgttta tttaatgcac caatgtaagg tttttctgtt aaaggctgaa agccttcaga
    14641 tttcaaacgc tccatttcag cttctatatt ttctacttca aagcaaacat gatgaatacc
    14701 ttcgcctttt ttttctaaat atttatacac tttactttct ggtgtatgtg cttgcattag
    14761 ctccacttta gtatctccta aatcaaaaaa ggaaacatct agtttttcac tgattacttc
    14821 ttcttggtga aatggtggct tattaaataa ttttgtaaaa agctcgtttg acgatgctaa
    14881 atcttttacg gctatgccga tatggtctat ttttttcata ttgataccaa tttagaagta
    14941 ataaaatgat tttacaagca tttaatattt taaccatatt gcaattaact ttaactgacg
    15001 ctaagcattc gtcgtgaaaa aatatgttca aaaatgtata attttacttc gtgatttatt
    15061 tagactataa tgcaacgaca ccggtggacg aacaagtgct tcaaaccatg ttgccttatt
    15121 tctctgaaaa atttggtaat gccgccagca gtacgcacct tttgggcgca caggcaaaag
    15181 atgccgtcga aattgcgcgt caccatgtag ccaataccat tcattgcgaa gcgcaagaaa
    15241 tcatatttac ctcaggaagc actgaagcca tcaaccacgc aattaaaggt gtttttcaat
    15301 tgtatcagca taaaggcaaa cacatcgtga ctgtaaaaac agaacacaaa gccgtgatag
    15361 atacttgcaa atatctcgaa aaaaaaggcg cgacaattac ttatttaaat gtagataaag
    15421 atggattaat cgacttagat gaattagata aggccatttc tcatgaaacc attttagtat
    15481 gtgtgatgta tgccaacaat gaaacaggcg tcgttcaaga cattaaaaaa atagcacaaa
    15541 tcacacatca gaaaaatagc attttctttt gcgatgccac acaagccatc ggaaaaatgc
    15601 caataaacgt gcaagcagat ggtatcgacc tgatgtgtct ttcggcacat aaaatgtatg
    15661 gaccaaaagg cattggtgct ttatttgtac ggcgaaaaaa tccaagagta gcactcgaac
    15721 ctttgttgca tggcggcggc cacgaacgtg gctggcggag tggcacgctc aacgtgccgg
    15781 cgatagtcgg cttaggaaaa gcctgcgata tcattggcga aaacaaattc atcgaaaaac
    15841 taagagatga attagaagat gcattgctag cacattatca agataaaata aacattaatg
    15901 gcaagcacgc aataaggctt gctaacactt ctaatattac atttccattc aaagccgtcg
    15961 atttaatcag acaaacaaaa tcactattgt ccatcgccac aggctctgcc tgcacatcgg
    16021 cagaaaacac accatcgcat gtgcttacag ctatgggttt atcgcgtgaa caagcagaaa
    16081 aatcaatacg tttcagtttg ggaaaatata caacagaaac tgaaatacag acagtcctta
    16141 cattgataaa acatgctttt taattcggtt tttatttttt acggatatat ttttccgttt
    16201 gtaattccat atacttccaa ctcagcgtga gtgtgtctcc attgttcgat aagaaatatt
    16261 gtgctggtcg ctcatcatta aactgtacca tgtcaatgtt gttttcttta aacattatca
    16321 ccgtgccact cctaggcaat gctgtcgttt tagcatgtgt ttctaaaaat gtgttatcac
    16381 ttttcaactc caatgtaata gcatcatttg tattttctgg attagaaata ccacggcgtg
    16441 caccacagca atctgtttct ttccaaatcc aattgccata gatattgaca agtggcttcg
    16501 gcttaaatgg atccacccaa tccgttcctt ttttgggttt tggcatttta ggtggttttg
    16561 ccaagagcaa tgcagatgca ctcaaaacaa taaaaagtat aaataataat ctacgcatcg
    16621 ttatttttgt tttgtaaaag tataaaattt gtagttttta aattgctatg caaatatatt
    16681 gccaaaaata tgaatgaaat acaaaaaatt agaatagaca aatggctttg gtctatccgt
    16741 ctttacaaaa cacgtacttt agccacagat gcttgcactg caggaaaggt aaaaattgac
    16801 ggcgagactg tcaaagcatc atatctatta aaagtaggac aaatcattaa cataaataga
    16861 caaggtgaaa aatggacgct caaatcgatt aaattgatag aaaaaagagt aggtgcacct
    16921 attgccatta cctgctatga agacttaaca ccaccagaag aaaaagataa actcaaattt
    16981 ccttccgtgt tttttgaagt tagggacaaa ggtactggca ggcctacgaa aaaagaccgc
    17041 agagacatcg acaaattcaa agatatagag gactaacatg gtgaaacatg tcgcatatct
    17101 attgtttatt gtatttaaaa gtcgataatg ttctattata caacttcctt tctttaaata
    17161 ttaataacaa actaagtcca ttaaaaacaa cattttaata ctaaaaagta gtaaatttgt
    17221 acaaataact taagtatgtt tggaatgcac ggacccgaac tttggttcat cttagcaata
    17281 gtagttttac tttttggtgg taaaaaaata cctgaattga tgcgtggcgt tggtaaagga
    17341 attcgcgaat ttaactcagc gaaaaacaat cttcaagatg agtttgaaaa aggactaaaa
    17401 gaagacgaga gcaaaaaatt gccagaaagc aagtaattta atttaatact acgtggaagt
    17461 gtacagatct ctaaagacgc tgcaagaaga tttatataat aaaaaaatca cagcaactgc
    17521 gcttacacaa caatctatct ccaatatttt agcgcagaaa gaactcaatg cgtttgttac
    17581 agtatttgaa gaaagcgcat tggcaagtgc caaaaaaata gacgaaaaat tacaatctaa
    17641 tcagccgtta ggcaaactat ttggtttaac gattgctatc aaagacaata ttgtttataa
    17701 aaatcatatc gctacaggcg cttcaaaaat cctatcaggt tttatttcgc cctattctgc
    17761 aactgtagta gagaaattat tggcagaaga tgccatcatc attggcagta ctaactgcga
    17821 tgaatttgcg atgggtggca gtaatgaaaa ttcatattat ggtgctgtca aaaatcctgt
    17881 tgacaaagaa cgtgtttctg gtggctcctc tggtggctct gctgtggctg tcgctgccaa
    17941 tttatgttta gcagcattag gctctgacac gggtggttcg attcgtcagc cagcttcatt
    18001 ttgtggtata gtcggtttga aaccaaccta cggcagagtt tctcgttatg gattgatggc
    18061 ctacggttct tcattcgacc agattggcac atttacacat actattgaag atgccgcttt
    18121 gattttagaa gtcattgcag gtgctgatgc ttatgatgca acttgttctc cgcgagcaat
    18181 tcctagttat tcaaaccata tatcatccaa taaaaaatat aaaattgcct attataaaaa
    18241 tgccatcgaa catgctggcg ttgacaagga agtaagtgcc gccaccatgc aatgtattga
    18301 aagattaaaa agtgaaggtc accaagtaga tgccattgca tttgatgagt tagattatat
    18361 cgtgcctgcc tattatgtat taaccacagc ggaagcatct tccaatctgt cgcgttatga
    18421 tggtgtaaag tttggctatc atgccaaaga agctactgat ttagaatcta catataagct
    18481 aacacgctca caaggatttg gcaaagaagt acaacgcaga attatgagtg gctcatttgt
    18541 attaagtgct ggctactacg atgcctatta ttccaaagca caaaaagtaa gaagaatagt
    18601 agcagaaaaa acctacaata ctttagaaga atacgatttt atactatgtc caaccgcacc
    18661 aacaccagct tataagattg gcgaagtgca agattccatc gcttcatttt taggtgatat
    18721 atttaccgtt caggcaaata ttgcaggcat tccaggcatt tcattaccat tatatacaac
    18781 atcgaacggc ttgcctattg gcattcagct gatggcagga agatggcagg aagaaaaatt
    18841 gctggctttt gctaatgctt tgatgtagtg caattacttc acatttttat aaacatataa
    18901 cgtagtatcc aatgtgctta aaaattgctt gctggcatag gcgaataatg tttgcttagc
    18961 tttcttattt gtaataaata caggtgaata ttgcttttta aaatctgtgt cattgtaaat
    19021 gtgtcgtata acttttccgc caaaaccatt aacaatgtaa tctctttttg taaaaggaat
    19081 aaattttgat gtatctgcaa caaactcaaa taaaaataaa tctggttttt gttttaaaaa
    19141 tacagtttta ttaaatgcac ggtgtccttt taaaatatat ttaggtcggt cattaattgt
    19201 atgcgcaacc tctatattat ttaaacctaa gacatcattc acatatccac tataggtata
    19261 tgcaaaaccg cctgcagcaa ttacacctac tgatggcttc ttctgataat caaaaaatga
    19321 attgagatta ttgcctaatt ttctgttttc attcgcaata tcaaattcaa ttgcaatgga
    19381 atatttgtta ttaacaaata aaacgtaaag actcttaagt ggtagaaatg acaaaatcaa
    19441 tattgatagg aatatcttct ttcttgatga tgtaatcttg tttatcaatt tattctgatg
    19501 gctgaatgat agcatcaaaa aaagaaaaca caaatgaaaa atcggactaa atgcttgata
    19561 aaatcgagac atgcggaaat gatcaccgcc tgtatagaat ggaataccaa taccaactaa
    19621 agaaattata actaaggaga aaattatttt attgtttgat gtgtaatctt tttcatttat
    19681 catttttata aaccagataa atattaatac aaaaaatgca ataattaatg gattcgtcct
    19741 actaacatat ttcccaaaat aaattgcacc ttcaaacaga ttatttaaaa atccatcaga
    19801 catttttaca taatatgtat taggaaatgg atagccaaaa taatataaac gccattttgt
    19861 taatgctatt aatgatatta taaaaacaac acacattggt attgtatgaa tgagtatttt
    19921 cctaaaagaa attttttcca aaaacaattg taaaacacga atagctataa aaaccaatcc
    19981 tattaatata ctttctggtc gcgtaataat aaaaaatgga aaaagtagtg atattattat
    20041 agttgatttt tgaaaagaaa agttattgtt taagtatggc actattaata agtaagtaaa
    20101 taataccaat tcaaaagtcc acaagcctgt ttctaaaagt gataatactg tccaatcata
    20161 aaagccggga aatacgacta aaaatcctaa aaataaagtg atataattag cagacaagtg
    20221 ctcatctttt ataaataatt caatgatctt aattgcaatt ttgagtgtaa aaaaaagtaa
    20281 aacaacatta ataataagca aacatatttc tatgtgattg gtaaatataa aaatgaaact
    20341 accaattaat gtccataata tagaggtaaa tccttcaaca ctttcactgc ctacattata
    20401 tacaaatccc ttgccatcag caaagttttt catatacaca aaatagatat ttgcatcatc
    20461 tattccgatt ttaggaaaac catataagca gccgatgata aatatcgata ctaataaata
    20521 aatgaaaatt aggatattct ttttcatcag aaactattca acaataaatt tatctgtatt
    20581 tgtattttcg atttcatact attcatacat aaaactgctt gttggatagt ggctcatatc
    20641 tgaattgaga aaatctatat atataaatat tgctttaaat attagaatac aaatatattc
    20701 tgatattctg aaaactaata acattcctaa aaaaatccta aaaaacattg atttatcctt
    20761 tttaaggttc atttttctta tcttgcaaaa atgagcattc gactatattt tactatatat
    20821 atactattct ttttctttgg ttttaatcaa gaacttaagg cacaatcgcc tccaccttcg
    20881 caatacaaag atgcaccatc agaattatat attcctacgg aagagcaatt atttaaagac
    20941 gatcctgacc cagtagtctt gagtgcacaa attgcagtgc gcttaaaaaa aatacaacaa
    21001 atcattcctt tagattataa cgaccacgtc caaaaataca tatcgtacaa tacgcacgaa
    21061 aagcgcaaag aacatatcgc aaaaatgttg gcacgcgcta agaaattctt tccaattttc
    21121 gagcctatat tagcaaaata tggcattcct gatgaaatga aatacttggc tgttgtagaa
    21181 tcagcactaa atccatttgc cttgtcgaga gttggtgcca gtggtccttg gcaatttatg
    21241 tatagcacag cattgcggta cgaactcaac atcaacaaac gtgtggacga gcgccgagac
    21301 ccttatcttg cttgcgaagc ggctgcaaaa tatttgagtg aaatgtatgc attatatggt
    21361 aattggcaat tggcaatcgc ctcttacaat tgtggtgctg gcaatgtcaa caaagccata
    21421 aaaaaagctg gtggttcatt gaatttttgg aatatccgcg gatttcttcc tgcagaaacg
    21481 cgcgcgtatg tgccttcatt tattgcagtc gtttatgcca tgaaatatgc tggaaaatat
    21541 ggcattagcg cattgccttt gaatagagaa aatacgcagc gtattaccat cagacaaaaa
    21601 atatctgttt ctgatttaga aagcagttta tccattcctc gacaagatat tattgatgaa
    21661 aatccatcgc ttttaacgac aactattcca gcagatttta ttctcaatct gcctgtttct
    21721 aaaataaatg cctactatca attccaagat tccttgcaac aaaaagcgct ttgtgaattg
    21781 gaagaaaaac aattagttgt acctatggta attaaaccaa ctagttcaga cgcggaaata
    21841 tcagcatcaa aaaaatcaaa tgtttctaca aaagaggaat ggaaaaatat tccaacagca
    21901 gaaaaagcag catcgaatgt cagcatcgac aaacctttag ggaaagaaga tgatgtcaac
    21961 tataaaacaa tggcatacac cgtaaaaaca ggcgacaatt taggaaagat agcggcatgg
    22021 tttcattgca cagtaaaaga attgcgcgct tggaataaac tacaagacaa taatattgct
    22081 gtaaaagatg aattactttt atacattcac aaaaacgatt ataaaaaatt tgttcgattt
    22141 aactatttaa gcgctagcat caaaactgat ttaagtgcaa aaattacaac acaagaagag
    22201 aaaaacaata cactacaaca agaaaaaatt gaggccaaca cacctttgat tgacaaaata
    22261 aatcctataa aaattttaag taaaaaagat tgttataaaa aacatacggt aaaaagtggc
    22321 gaatcgttat atagtattgc tcaaaaatat gacgatgtga gcgttcaaga cttgctatta
    22381 tggaatggct tcaaaaaaac accaattcta cataaaggcg acattattaa ggtgcgtaaa
    22441 attgcctgca aataagctat ttcctaatca taaataggaa aaacaaaaat cggatagcgt
    22501 atttgtaaat cgctacaatt tatagataag tttattattt ttgttcaata tcacaaattg
    22561 actattaact ataaactatg tttgcatata taagcggaaa aattacttat aaaacaccta
    22621 cacatattta tctggattgt aatggaattg cctattatat caatatttct ttgaatacgt
    22681 atgcggctat tgagaagcag gagtctgtaa agctctacac acaccttgtc gtccgcgaag
    22741 atgcacatgt gctttacggc ttctatactg aagaagaaaa aaatttattt ttacatttaa
    22801 tatccgtttc tggcgttgga cctaacacag cgcgtttggt gctgtcatca atgtccactc
    22861 aagaatttca aaaggcaata attcaagctg atgtgcgttt aatacaatca ataaaaggca
    22921 ttggaccgaa gtcggctcag cgcctaattt tagaactaaa agacaaatta aaaaaagacg
    22981 caacagctga aggtatggat aacattgcgg caagcagtag caacacgctt atcaaagaag
    23041 aagcattggc tgccctaaat atgctcggtt ttcagaaagc acaagcagaa aaggccgtac
    23101 aaaaatccat gcaagaaaat acccaaatac agtcagttga agaattaatt aaattgagct
    23161 taaaaaattt gtaaatttgc cacacacact cgatacttag tgataaaaaa actacatact
    23221 acttctaaaa gtactctcct attttttgct gttttatata gcacgataaa tgtatttgct
    23281 caaacggata gcaacaaaac tacattgcgt ttcccaattg aagatagaa
//
LOCUS       DAOQQV010000030        22355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_691_length_22355_cov_6.976099, whole genome shotgun sequence.
ACCESSION   DAOQQV010000030 DAOQQV010000000
VERSION     DAOQQV010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22355
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_691_length_22355_cov_6.976099"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..682
                     /gene="fsa"
                     /locus_tag="PLL59_04920"
     CDS             23..682
                     /gene="fsa"
                     /locus_tag="PLL59_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HMY23180.1"
                     /translation="MKFFIDTANLEQIKEAADLGILDGCTTNPSLMAKEGITGDKNVW
                     NHYKKICEIVDGDVSAEVIATDYKGMIEEGKKLAKLHKNIVVKVPMIKEGVKAIKYFS
                     DNGIKTNCTLVFSPGQAILAAKAGATYLSPFVGRLDDINQDGIELIQQIRLIYDNYQF
                     DTQILAASVRNALHIVRCAEAGADVVTCPLSSIMGLFHHPLTDKGLAQFLADHAKANK
                     K"
     gene            703..1257
                     /locus_tag="PLL59_04925"
     CDS             703..1257
                     /locus_tag="PLL59_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HMY23181.1"
                     /translation="MFWTTLHTTYKLLKQISFVFVALFLFISCDSLVSKNDSYNLGYK
                     DGYVAGLKAGKEEQQKSIADKNKKVVIDPAKNDIPTIPEFVEDGHTKNDKISVPEKAL
                     KVLDFIKANKKAPEGYVGGRHFGNYERRLPERDAAGNKVSYQEWDINPKVDGRNRGPQ
                     RLVTGNDGSAWYTPDHYQTFREIK"
     gene            1258..1590
                     /locus_tag="PLL59_04930"
     CDS             1258..1590
                     /locus_tag="PLL59_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HMY23182.1"
                     /translation="MQTFKSNIVVNGIFLAFIDGQKCAVMTHFYEEIAKALNFPDYFF
                     PNLDSLDECLNDLSWIEQNEIHLLIFNYEDLLFEESYSLDVINNIFANAMSELEENGK
                     HFSVFTQQ"
     gene            complement(1591..2016)
                     /locus_tag="PLL59_04935"
     CDS             complement(1591..2016)
                     /locus_tag="PLL59_04935"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMY23183.1"
                     /translation="MNSTVFNEIKFPFPYTQQVAWGDMDAFNHINNVIYFRYFETGRV
                     EFFNKTNLWQLFDDENIRIVVAKLECNYVQEIKHPATIEIAVGIKKIGNTSLIVHHIV
                     KCNGNIAAHGEGIIVATNPATGKSTPWTEKLRANFEQWK"
     gene            complement(2086..5166)
                     /gene="secDF"
                     /locus_tag="PLL59_04940"
     CDS             complement(2086..5166)
                     /gene="secDF"
                     /locus_tag="PLL59_04940"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HMY23184.1"
                     /translation="MGKGTRTALVALTALIVLICIYCLSFTFKVNSIEKEAIALAEKA
                     IPMQDLDKRYPGNQLAQFLYEDSIHQSRINHKNRYLDSISNEKVYLWNTYKACKEQQL
                     NLGLDLQGGMSVVLQVSLKELLSTLADNSRDPKFNKALDVAEQRMSSANNDYVSLFLE
                     AYKEQNPTTTLAQLFSTRNNKEDINAESSNSDVEKFIRAKAKEAFDVTLNRLTTRIDQ
                     FGTKQPTITSEPARERISVEIAGVDNPARVRKLLQATANLEFWETYKATEIGKSLNDA
                     NTVLKNYLDTQKDLGKDSAVMSKAQSLLTASTDSSAEAVNPLSGKKDTSKLAKKDTSQ
                     AKRFEEFKNENPLFAVLQPAVDENNQFQASPIIGYVSTLDTAKLTEYLTNPDVRSVFP
                     KDLDFSISAKSFGDKQKFFTVYAIKRNTNDGNAPLDGSVVTQARQDYDQNGQVVVSMQ
                     MNTIGARKWRDLTRKNVGNSIAIVLDNQVYSAPNVNGEIDGGSSQISGGFDIQEATDL
                     ANVLKTGKLPASARIIEEEIVGASLGSSAISAGLYSLLASLLMIIVFMVFFYSSAGVL
                     SIFVLLLNVFLIVGLMAGIGSTLTLPGIAGIVLTLGMAVDANVIIYERVKEELRAGKG
                     YAYAAQEGFKHSLPAILDGNITTLLTGIILAWIGIGPVKGFAVTLIIGILTTLFTAVL
                     LSKMIVDARLEKGKHFEYFTPITKNFLQNINFDFIGKRKITYTISTIFIIIGIVSFVT
                     RGFDLGIDFDGGREYKVRFENPVNTDEVRDVLDKTLGSGNVVKQFGAKNQVKITTKYM
                     INNSSKDADLKVEDAVYKALIKYLPNTSFENFKRVNLMSSTKVDPSMTGDFKRSSMWA
                     AIASIIVIGIYILIRFSKKGYVFGSIAATAHDAFAMLAVFSIFHGIMPFALEVDQIFI
                     AALLTIIGYSINDTVIIFDRLREYLKERPNADMKDTMNAAINSTLSRTFNTAFTVFIV
                     VFILFIFGGPVLRAFSFAMLIGVVVGCYSSVFIASPIIYDLDKNNNTPAKTTKK"
     gene            complement(5478..6863)
                     /locus_tag="PLL59_04945"
     CDS             complement(5478..6863)
                     /locus_tag="PLL59_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMY23185.1"
                     /translation="MTLVQSRLPRLNGNNLLIILLILSGISSCASSKITSSKQVETVV
                     IQKKNTETVAINTKKKEDSITINPSSFDLDDNLNKNTSTNTKSTTYTNQNLTSDINRI
                     YNIAIILPFNLNQISLGQYADDTTKQLKPDAKNAVEFYLGCQLAKEKFDNSDLKANVY
                     FLDDNNDSTTLSQLFNQKPFPNVDYLLGPLNFKNLKQLATYANKTQTTLISPFANSMY
                     IANNPYYFNATPSLIQQYTYALNQIHNKFPNQSIDVLYDALDSTAESINILKKLVNNN
                     QHQLKVKYTSVQASSNAAKLIATADTLSNKTILIYSSKDTYIKSVVAKLKPSKNKLDI
                     FTSANARFIKALSDNKNPHSVYTVYPFNNDNSNYEAFASKFEEKYNKKPSEIAAQAFD
                     ILTHLLNTIDKKQTLQNNIYQYSTDFNNVHTKFRFEPVLDNEKNVDFYDNSFLYLYKY
                     VNGTFVLETPN"
     gene            complement(6820..8358)
                     /gene="guaA"
                     /locus_tag="PLL59_04950"
     CDS             complement(6820..8358)
                     /gene="guaA"
                     /locus_tag="PLL59_04950"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HMY23186.1"
                     /translation="MHSKILIFDFGSQYTQLIARRIRELNVYCEIHPYNHLAVLEKKD
                     FNGIILSGSPFSVLQDDALQFPIKEVQATYQVPVLGVCYGAQYIAHVFGGKVERSSKR
                     EYGRAHLDFIAADSAFFNNIQTKSQVWMSHGDSVLALPAQFELLANTDSIPVAAFKHS
                     EKEIYGIQFHPEVTHSTDGKTLLQNFVVDICGCRQDWTPLSFIEETVTEIKQTVGSDH
                     VVLGLSGGVDSSVAALLLHKAIGNQLVCIFVNNGLLRYNEYQSVLDSYQHLGLNVVGV
                     DASARFYEELKGIAEPEAKRKIIGKVFIDVFQEEAEKLKNVKWLGQGTIYPDVIESVS
                     VHGPSAKIKSHHNVGGLPEKMHLKIIEPLNRLFKDEVRRVGKALELPENILHRHPFPG
                     PGLGIRILGDVTAEKVEILQKADYIFIEGLKKDGLYNDVWQAGVMYLPVQSVGVMGDE
                     RTYENVVALRAVSSIDGMTADWCHLPYEFLARVSNEIINNVKGINRVVYDISSKPPAT
                     IEWE"
     gene            complement(8486..12031)
                     /gene="smc"
                     /locus_tag="PLL59_04955"
     CDS             complement(8486..12031)
                     /gene="smc"
                     /locus_tag="PLL59_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HMY23187.1"
                     /translation="MRLRSLDIKGFKSFADKTQINFDGNITGIVGPNGCGKSNVVDAI
                     RWVLGEQKTKALRLEKMDNIIFNGTKDKSASNIAEVSITLDNTRNLLPTEFSSVTITR
                     TVERDGDSDYKLNGVSCRLKDIRDLFMDTGISTDTYAIIELKMIDQILNNVDNARRTL
                     IEQAAGISKYKTRKKETLAKLQATEGDLNRVEDLLFEIDKNLKSLESQAKKARQYKNF
                     KEEYKVVSIDLSKYELHGINESFLLSNKEIEEQEDKRLEANTQIEVLEANLEKEKLAV
                     LDKEKSLSDQQKQLNSFLSQLQEKESQKKIDQQKSSFLKNQIVQLNQQINQAKTIIES
                     LSSEIANLKNYNEEDEHKLEIVRVEFDDAKQKLEEQRNTNLEFRNNLDEARNKHNSCA
                     NKITETEKLIAVKETQYESLQRSAQQSIFENEERVKTLEALHTDISNTQSKIEKQKAL
                     VDDLRDKEEENIEKIKTLEEKNEQLRQELTNKNRILDAKNNEYKLTKNMIDNLEGFPE
                     SIKFLKKNVNWLKDTPLLSDIIYSEEKYRVAIESVLQPYLNNFVVDSENEAINAIDIL
                     SQSSVGKASFLILDYFKKAVISDNHNNIQSAKKAIDIIQIDEKYQPILAYLLHGVYIA
                     DEGADVNHIFDNVAQKKDVFIISNSGHLLKSDKQISGGAVGLFEGKRLGRLKNLEILE
                     KEIKELETEVLQFKKVTQDNYNELQHLKVNSFRNVIERESHELQQIEKELVAKKSKIE
                     SEESSIGKISSQAQALQQQIDALQLEMEPLKTTLEEHINTANTYKSELDNLEINFRKA
                     NELYNQFSQAFNQANILFIQQENKLKSNQQTLTYKQHQLEENQNRLENAVKEIENSQL
                     EIDILSESIDALETVLMESYKEKELQMQSLSTQETDYYKVKEGITSIEDAIREKNKNR
                     QAIEQNIMSKKELLSELKLKLNVLKERLSIEFHIEVEEFANNLSLPETPKDDLFDRLE
                     KIKRRIENFGEVNPMAEEAYNEMKERFDFITTQKQDLLDSKENLMQTMLEIENTAKGQ
                     FLETFNAVRENFISVFRSMFTEDDNCDLVLVNPDDPLESEIDIIAKPKGKRPQGINQL
                     SGGEKTLTALSLLFALYLYKPAPFCILDEVDAPLDDANVRKFNEAIRKFSQNSQFILV
                     THNKSTMASVDSIYGVTMVKQGISRVVPVDFGSLN"
     gene            complement(12245..13264)
                     /locus_tag="PLL59_04960"
     CDS             complement(12245..13264)
                     /locus_tag="PLL59_04960"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HMY23188.1"
                     /translation="MSFQDKLIFIVGNSRSGTTMMLRIFNMHPQLMVLNELHFFEQLW
                     SPEDKGKIITKEGAITLAAKLLLTQRIGYMTHDQNFSQFNEEATLLINEINKPDVTAE
                     DVFHYFMQREVALHGKTIICEKTPQNVFYLSEIFELYPNAKVINMVRDPRAILLSQKN
                     KWNRRNLGGDYMTRKEALRLRINYHPITLSKLWNAAIHAAKLFKQDKRVMFIRFEDLL
                     EAPETTLRSICNHIEVAFDSNMLNITQESSSIEKDSKEIGFKKERASNWKKGGLNESE
                     RWICQKMCRSNLLKNNYEIENIKPNIFYIAFYLISFPIKIMLALLMNLNRMKNISETL
                     KRRLR"
     gene            13358..14950
                     /locus_tag="PLL59_04965"
     CDS             13358..14950
                     /locus_tag="PLL59_04965"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMY23189.1"
                     /translation="MNYHINTKYTKSISSCQQHCFIVVSLFFMLLYHNVNAQKIIAGP
                     IIGAVSDSSAKLVFMTDKPIHATIKCISTADTLIQTHRTSTDDVYYVNACQINSKLKS
                     NTNYEIQINITGNDSTYISSFKTFPSTKETSLFSFTLGSCTEQHYNDSIFFEMKKHKP
                     LFFLQIGDWLYANNFNERKFYYAESIAHQKELYEKRYEMPNLKNMLASTPIDFMFDDE
                     DAVFDDFSKNTYTHVQQVANATQISEIAYPDSLRKNVIEGYHTFFPYYNHLPNQAYHS
                     FVCGNAEFFVVDTRSTRDANTASFVKKNKHYAYKVPNQHLLLDTLQWNWLLSSLKKSQ
                     AQWKFIVSGVTFNKSYKDVFDIALLKPAQNKKLANGMTGLSIAAGLSTMWFAFPETQI
                     ALLNFCHDNQIKNVIVLSGDAHTAAIDDGANAGFPELMAGGLAQKNSRLAAIIKNQLH
                     LNLWNQGGQGIGNNNFNDAFGKVDINGNQSVVLSCIDKYGKTICSYEVKDGFIPKKYT
                     LKPMLSINKIRAFKHALRIKLK"
     gene            complement(14953..15972)
                     /locus_tag="PLL59_04970"
     CDS             complement(14953..15972)
                     /locus_tag="PLL59_04970"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HMY23190.1"
                     /translation="MQNEIVQAGNLLDKNGILIQKGWARDLILTYNRSDIKTSKYSIK
                     EWDYYCILQDKYGIAITVADLAYVGVLAVNLFDFTNNTEISDQVLIPFPLGKMQLPAQ
                     SSVGNIIIKNKNINIAINIKDGNRNIEIQYPGFAKGKGLQASIKLLTINKDSMVIATP
                     FIENKRAFYYNQKINCLAASGSFTYNGNQYHLNPKTATAVLDWGRGVWTYQNRWYWGS
                     ASAYVNGKPFGFNIGYGFGNTSAATENMLFYDGVAHKLDQVVFHMDTSNYLKPWKFSS
                     NDKRFELDFEPAIDRYSNTNLILIKSVQHQVFGYFSGRVVLDDGTILQVQRVLGFAED
                     VFNRW"
     gene            16040..16915
                     /locus_tag="PLL59_04975"
     CDS             16040..16915
                     /locus_tag="PLL59_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMY23191.1"
                     /translation="MSNLNVKHEYIEVNGIKLHVAKQGNGKKLVVLLHGWPEFWYTWR
                     YQIPELAKHFTVYAPDLRGFNLSDKPHGISNYKTDTVASDIAALVQQSGFKKALIVGH
                     DWGGAVAWTFATMFPELTEKLVVCNCPHPKLMLDSFKSNPSQLIKSWYIFMHQIPLLP
                     EMIYQFTLPLFFKQFVRGWMYNKHNFSDDDLNEFVKAFKHEGALTGSINYYRAMLQTK
                     PNLKIFDKKIQSPTLLIWGEGDKALGKELTYNTEKYIDAAYEVKYIPNCSHWTQNDCP
                     ELVNEFLLDFFITKK"
     gene            17066..18031
                     /locus_tag="PLL59_04980"
     CDS             17066..18031
                     /locus_tag="PLL59_04980"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMY23192.1"
                     /translation="MLYNSPILKSPKWLYTKHLQTIIPNVYRKVEGVFYQRERIETWD
                     DDFLDLDWSKVNSNKIIIISHGFEGSSDRVYARGLTKILNANGFDVCVWNFRGCSGED
                     NRQFFGYHSGKTDDLDYVVKHILTTKNYDEHYMIGISMGGNLTMKYLGEDKWETAKKI
                     VSALTVSVPIHYETSFEHLCKGWNLIYERRFTKQVKDKVLKKITKYPDAIDYKYVLEA
                     KNLWEFTARCTVPMHGFTDIHDYNKKVQASTYIRNIQTPILLINADNDPLLTKECYPT
                     QVAKKSNFFHLEITIGGGHVGFCEDIKAEHNWLENRVVTYFTKGY"
     gene            complement(18037..18183)
                     /locus_tag="PLL59_04985"
     CDS             complement(18037..18183)
                     /locus_tag="PLL59_04985"
                     /inference="COORDINATES: protein motif:HMM:NF037605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HMY23193.1"
                     /translation="MDELVVNIDYYIDENGNYVFTKAYHQKRGYCCGNNCKHCPWKDE
                     EDNN"
     gene            complement(18183..19266)
                     /gene="prfB"
                     /locus_tag="PLL59_04990"
     CDS             complement(join(18183..19205,19207..19266))
                     /gene="prfB"
                     /locus_tag="PLL59_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131330388.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HMY23194.1"
                     /translation="MTNEQYKLLLERVASFGRYLDVANKIEQIKDDEAKTLAPDFWND
                     QLKAQALMKEIKQNKHWVTLFKSVETKFNDLNVLREFQEIGEASDEEIDLQYQAIIDE
                     LDENEFKSTLKNEEDNLSAIIEINAGAGGTEACDWASMLMRMYTMWANKNGYKITELD
                     YQAGDVAGIKSVALEIAGDFAFGMLKGENGVHRLVRISPFNAQGKRMTSFCSVFVHPV
                     VDDSIEIEINPADLEWDVFRAQGAGGQHVNKTESAVRVRHLPSGIVCECQTQRSQIQN
                     RETALKMLKSKLYEAELQKQLEARDAIEAQKMKNEWGSQIRSYVLDDRWVKDLRTEYK
                     VTNPDKVLDGDLNGFFKAYLLHKEQK"
     gene            complement(19293..19637)
                     /locus_tag="PLL59_04995"
     CDS             complement(19293..19637)
                     /locus_tag="PLL59_04995"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HMY23195.1"
                     /translation="MPTSNQIGDAGEKLALEFLQKKGYTILFSNWRSKRAEIDIIAQY
                     QRVVIFVEVKKRSSSLFGTPESFVDKHKIKKMREAADAYIELYDWKGELRFDIIAIEK
                     NNMITHFEDAFY"
     gene            19758..20747
                     /locus_tag="PLL59_05000"
     CDS             19758..20747
                     /locus_tag="PLL59_05000"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HMY23196.1"
                     /translation="MDDLIQGFPAQLKRAIEIGESAKIGKNKFPIRNILISGLGGSGI
                     GGTILANVLRDDVAMPILVNKEYQIPAYVNENTLVIISSYSGNTEETLSALMQAFKKN
                     AQIVCITSGGLVQEYAETNDIDYVLIDGGAPPRSAFGQSFVQLLYIMHHLGLLEDGFK
                     KYLQEAITLLESEEADIKAQAKNIAQKLNGKIPVIYSDAKFEGVCIRFRQQINENSKM
                     LCWHHVVPEMNHNELVGWREKNEQIAVVFLRNNKDFERNQERMEFTKEVVSQYASDVV
                     EIYSKGDYDLIRSIYLIHLTDWVSFYLAELKGVDAIEINVINKLKSKLAENPF"
     gene            20811..21893
                     /locus_tag="PLL59_05005"
     CDS             20811..21893
                     /locus_tag="PLL59_05005"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HMY23197.1"
                     /translation="MSRIYNTIGSLTTLKLKLKDNQINDFHSLNEVINFQKSFHAIKQ
                     EILIKHEDFIEREKKLLVNDLQNLESEIITLEKQYKDRFALEIGGLKQELVNVETILS
                     NNFIQKIRFLIKKWILKRKIRDKEFDFNRQVEMDINLRIEKLNQKKSRFQFITLNTKQ
                     AVVLSAQKAILELERKKAIVDSLNSYIYGSIGENKVVKTLDKLSDEYYLINDFSFTFS
                     PAIYYNQEDEYIKSIQLDHILIGPSGVFLIETKNWSEKSIDNLDLRSPVCQIRRSSFA
                     LFKLLNSESDDLGLQLNSHHWGSVKISIKNLIVLLNSKPKGEFQYVKILTLNELIGYI
                     SYFKPIFTHSEVESVFKYIYRLNEQN"
     gene            21984..>22355
                     /locus_tag="PLL59_05010"
     CDS             21984..>22355
                     /locus_tag="PLL59_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate-protein ligase B"
                     /protein_id="HMY23198.1"
                     /translation="MQKIRVHDKGVIQYADALVLQTEKFDELIENKINNITNKDAHHL
                     FLCEHQAVITLGKSAKEQNILLPEALLKENNIDIFHINRGGDVTFHGPGQITGYPILD
                     LDFFTSDIKMYMRMLEEVMIRT"
BASE COUNT         7152 a         3669 c         3621 g         7913 t
ORIGIN      
        1 gtagtcaaaa tcaaacacat ttatgaagtt tttcatcgat acagcaaatt tagagcaaat
       61 aaaagaagcc gcagatttag gaattttaga tggttgcact acaaatccgt ctttaatggc
      121 aaaagaaggc attactggtg ataaaaatgt ttggaatcac tataaaaaaa tatgcgaaat
      181 tgtagatggc gatgtaagtg ccgaagtgat tgctactgac tataaaggca tgatagaaga
      241 aggtaaaaaa ttagccaaat tgcataaaaa tattgtggtg aaagtaccca tgattaaaga
      301 aggtgtgaaa gcgattaaat atttttctga caatggcatt aaaacaaatt gtacattagt
      361 cttttcacca ggacaagcta ttcttgcagc caaagctggt gccacttatt taagtccatt
      421 tgttggtcga ttagacgata ttaatcaaga tggtatagag ttaatccaac aaatcagatt
      481 gatttatgat aattatcaat ttgatacgca aatattagct gcatctgtgc gcaatgcttt
      541 acatattgtt cgatgtgcag aagctggcgc tgatgtggtg acttgtcctt taagttcaat
      601 catgggattg tttcaccatc cgctaacaga taaaggtttg gcgcaatttt tggccgacca
      661 tgcaaaggca aataaaaaat agtaatttta tttttatttc aaatgttttg gacaacatta
      721 catacaacgt acaaactgct aaaacagata agttttgtgt ttgttgcttt gtttcttttt
      781 atttcatgtg atagtttagt cagcaaaaac gatagctata atctcggtta taaagatggt
      841 tatgtagcag gcttaaaagc tggaaaagaa gagcaacaaa aatcaatcgc tgataaaaac
      901 aagaaagtag taatagatcc agcaaagaat gatataccaa ccattcctga gtttgtagaa
      961 gacggtcata ctaagaatga taaaatatca gtgccagaaa aggcattaaa agtactcgat
     1021 tttattaagg caaataagaa agcaccagaa ggttatgttg gtggtcgtca ttttggcaat
     1081 tatgaaagaa ggttgcccga acgcgatgca gcaggcaata aagtatctta tcaggaatgg
     1141 gatattaatc cgaaagtaga tggtagaaac agaggtccac aacgcctagt cacgggcaat
     1201 gacggaagcg catggtacac gccagatcat tatcaaacat tcagagaaat taaataaatg
     1261 cagacattca aaagcaacat cgtcgtaaat ggcatttttt tggctttcat tgatggtcaa
     1321 aaatgtgcag taatgacgca tttttatgaa gaaatagcga aggcgctgaa tttccctgat
     1381 tatttttttc ccaatctcga ttcgctggat gagtgtttaa atgatttatc ttggattgag
     1441 caaaatgaaa ttcatttgtt gattttcaac tatgaagatt tgttatttga agaaagttac
     1501 agtcttgatg ttatcaataa tatttttgca aatgccatga gtgaattaga ggaaaatggc
     1561 aagcattttt ctgtgttcac acaacaatga ttatttccat tgctcaaaat tagctcttag
     1621 tttttctgtc caaggtgttg actttccagt tgccggattt gttgcaacaa taatgccttc
     1681 gccatgcgca gcaatattgc cattgcattt tacgatatga tgaacgatta agctcgtatt
     1741 gcctattttc tttataccta cagcaatttc tattgtcgca ggatgcttga tttcttgcac
     1801 ataattgcat tctaattttg cgactacaat tctaatattt tcatcgtcaa atagctgcca
     1861 aagatttgtt ttattaaaaa attccacacg acctgtttca aaatatcgga aatatatgac
     1921 attattgata tggttgaacg cgtccatgtc gccccaagcc acttgttgcg tatatggaaa
     1981 gggaaatttt atttcattga atacggtaga attcatgttg taaattaatt aaaaagtcct
     2041 gataaaatca ggacttatat ttagaaaaat tttcagcttt atacactatt tctttgttgt
     2101 tttagctggt gtattgttgt ttttgtctaa gtcgtatatg attggagaag caataaatac
     2161 agacgaataa caacctacaa ccacaccgat taacattgca aatgagaatg cacgtaaaac
     2221 agggccacca aatataaaca agataaacac aacgataaat acagtaaatg ccgtgttgaa
     2281 tgttcttgat aaagttgagt ttatcgcagc attcatagtg tctttcatat ctgcatttgg
     2341 tctttctttt aagtattctc ttaaacggtc gaagataatt accgtatcat tgatagagta
     2401 accgattatt gttaataatg ctgcgataaa tatttgatct acctctaagg caaaaggcat
     2461 gatgccatgg aaaatggaga atactgcaag catagcaaac gcatcgtgcg ctgtagctgc
     2521 aattgaacca aatacatatc cttttttgct gaagcgaatt aagatataga taccaattac
     2581 aataatagaa gcgatggctg cccacatgga gcttcgtttg aaatcgcctg tcatcgatgg
     2641 atctactttt gttgaactca ttaagttgac acgtttgaaa ttctcgaaag aagtatttgg
     2701 taagtatttt attaatgctt tatatactgc atcttctact tttaaatcgg catctttaga
     2761 tgaattattg atcatgtatt ttgtcgtaat ttttacttgg ttttttgcgc caaattgttt
     2821 taccacatta ccagaaccta acgttttatc taatacatcg cgtacttcgt ctgtatttac
     2881 aggattttcg aagcgcactt tatattcacg accaccatca aagtcgattc ctaaatcaaa
     2941 accacgtgta acgaatgata caataccgat gatgatgaaa atagttgaaa tggtataagt
     3001 gatttttctt ttaccaataa aatcgaagtt gatattttgt aaaaaattct ttgtgatagg
     3061 cgtgaagtat tcaaaatgtt tgcctttttc tagtcttgca tcaacaatca ttttgcttaa
     3121 aagaacagct gtaaataatg ttgttaagat accaatgatt aatgtaactg caaatccttt
     3181 tactggacca ataccaatcc aagctaagat aatacctgtt agcaatgtgg tgatattacc
     3241 gtctaagatg gctggcaatg aatgtttgaa gccttcttgc gcagcataag catatccttt
     3301 tccggcgcgc aactcttctt tcacacgctc ataaataatt acgtttgcat ctactgccat
     3361 accgagtgtg agcacgatac cagctatacc aggtaaggtc aaggtggaac caattccagc
     3421 cattaaacct acaattaaaa acacgtttaa taagagtaca aaaattgata aaacacctgc
     3481 tgatgaataa aagaacacca tgaatactat aatcattaat agcgatgcta ataatgaata
     3541 taaaccagcg ctaatagcac tgctaccaag tgaagcacct acgatttctt cttcaataat
     3601 acgagcagag gcaggaagtt taccagtttt caatacgttg gctaagtctg ttgcttcttg
     3661 tatatcaaat ccgccactaa tttgtgagct tccaccatca atttcgccat ttacatttgg
     3721 tgcagaatat acttgattgt ctaaaacgat tgcaatgcta ttgcctacat ttttacgtgt
     3781 taaatctctc cattttcttg cgccaatcgt attcatttgc atgctaacaa caacctgacc
     3841 gttttggtca taatcttgac gtgcttgcgt aactacacta ccatctaaag gtgcatttcc
     3901 gtcatttgta tttcttttta tagcataaac cgtaaagaat ttttgcttgt cgccaaatga
     3961 ttttgctgaa atactaaagt ctaagtcttt tggaaataca cttctaacat ctggattggt
     4021 taaatattca gttagtttcg ctgtgtctaa tgtgcttaca taaccaataa taggagaagc
     4081 ttggaactga ttgttttcgt caactgctgg ttgtaataca gcaaacaatg gattttcatt
     4141 tttaaactct tcgaatcgtt tcgcttgaga agtatctttt ttagctaatt tgctagtgtc
     4201 ttttttgcca cttaaaggat ttacagcttc agcagatgaa tcagtagatg ctgttaacaa
     4261 agattgtgct ttcgacataa cagccgaatc tttgcctaaa tctttttgtg tatctaagta
     4321 attttttagt accgtattcg catcatttaa tgatttacca atttcagtag ctttataagt
     4381 ttcccagaat tctaagttgg cagtagcttg taaaagttta cgcacacgag caggattatc
     4441 aacaccagca atttctacag aaatacgttc acgagctggc tcagatgtaa ttgtaggctg
     4501 cttagtaccg aattggtcga tacgtgttgt taatctgttt aaagtaacat cgaatgcttc
     4561 ttttgctttt gcacgaataa atttctcaac atcgctattt gaactttcag cattaatatc
     4621 ttctttattg tttcttgtcg aaaataattg agctaatgtt gtcgtcggat tttgttcttt
     4681 atatgcttct aaaaataaag aaacataatc attgttggca gatgacatgc gttgttctgc
     4741 aacatctaat gctttattga attttgggtc acgactgttg tcagctagtg tagaaagtaa
     4801 ctctttcaaa gaaacttgca atacaacact cataccacct tgtaagtcca aacctaagtt
     4861 gagttgttgt tctttacaag ctttgtatgt attccacaaa tacacttttt cgtttgaaat
     4921 agaatctaag tatctgttct tgtggttaat tctactttga tgaattgaat cttcgtataa
     4981 aaattgagcc aattgattgc caggatatct tttatctaaa tcctgcattg gaattgcttt
     5041 ttcagccaaa gcaattgctt ctttttctat cgagtttact ttaaatgtga aagacaagca
     5101 ataaatgcaa atcaatacga ttaatgcggt aagtgctacc agcgccgttc ttgttccttt
     5161 acccatgatg gtttattgtt aaataagggt gcgaatatac tgcttttgag caaaatattg
     5221 aaatgaataa taaacaaatg ttattaatca atcgtaaatg atgagtttat gtgtataaac
     5281 ttctgtatta ttttgtattt ctatcaaaat atacaatttg tatggcaaaa aagagtagga
     5341 aatttagcga aacaataaac tgcaccatta gcgttttcta atgccaactt tgacttaata
     5401 gcttgttgca tggtaatgac tccaaccgat taagaataat agagaataaa cttctacatc
     5461 aaagtgctca acaagtatta gttaggcgtt tcaagtacaa atgtaccgtt tacgtatttg
     5521 tataggtata aaaaagaatt atcataaaaa tctacattct tttcattgtc taatacaggt
     5581 tcaaagcgaa atttggtatg tacattatta aaatcagtcg aatattggta aatattgttc
     5641 tgtaaagtct gctttttatc aatggtattt aataaatgcg tcagaatatc aaatgcttgt
     5701 gctgcaattt ctgatggctt tttattgtat ttttcctcaa acttcgaagc gaatgcttca
     5761 taatttgaat tgtcattatt aaaaggatac actgtatata cactatgcgg atttttattg
     5821 tcgcttaatg ctttgataaa tcttgcatta gctgatgtaa atatgtctaa tttatttttt
     5881 gatggtttaa gttttgctac aacagattta atgtaagtat cttttgatga gtatattaag
     5941 atagttttat tgcttaatgt atcagcagtt gcgattagtt ttgcagcatt gctactggct
     6001 tgtacggaag tatattttac ttttaactga tgttgattat tgttcactaa tttctttaga
     6061 atattaatgc tttccgctgt tgaatccagt gcatcatata acacatctat gctttgattg
     6121 ggaaacttgt tatgaatttg attcaatgca tatgtgtatt gttgtattaa tgaaggtgtt
     6181 gcattaaaat aatatggatt gtttgcaata tacatcgaat tggcaaaagg tgatatcaag
     6241 gtagtttgcg ttttgttcgc ataggttgcc aattgcttta aatttttaaa attcaatggc
     6301 cctaacaaat aatcgacatt gggaaacggc ttttgattaa atagctgact taaggttgta
     6361 gagtcattgt tgtcatctaa aaaatataca ttggctttca aatcactatt gtcaaatttt
     6421 tctttagcaa gctgacagcc taaataaaat tcaactgcat ttttagcatc aggtttgagc
     6481 tgttttgtcg tgtcatctgc atattgacct aaagaaattt gattgagatt aaatggcaat
     6541 ataatagcaa tgttgtatat tctattaata tctgatgtta aattctgatt tgtatatgta
     6601 gttgattttg tgtttgttga agtgttttta tttaaattat catctaagtc aaaactcgat
     6661 ggatttatgg taatcgaatc ttcctttttt ttggtattta ttgcgactgt ttcagtgttt
     6721 tttttctgaa taacaacagt ttctacttgc ttcgatgaag taattttact tgaagcacaa
     6781 gatgaaatac ctgataatat caataagata atcaataaat tattcccatt caatcgtggc
     6841 aggcggcttt gaactaatgt catatactac tctatttatt ccttttacgt tattgattat
     6901 ttcattcgaa actcgtgcca aaaattcata aggcaaatgg caccagtcgg ctgtcatacc
     6961 atcgatacta ctcacggcac gcaaagcaac tacgttttcg tatgttcttt cgtcgcccat
     7021 aacaccaaca ctttgcactg gtaaatacat tacacctgct tgccaaacat cattatataa
     7081 accatctttt ttcaaacctt cgataaaaat ataatcagct ttttgtaata tttccacttt
     7141 ttcggctgtt acatctccta aaatgcgaat gcctaatcct ggaccaggaa aaggatgtct
     7201 gtgtaagatg ttttctggta attccaatgc tttccctacg cgacgcacct cgtctttaaa
     7261 caatctgttt agtggttcaa tgatttttaa gtgcattttt tcaggtaaac ctcctacgtt
     7321 atgatgtgat tttatttttg ctgatggtcc gtgtacagac acagactcaa tgacatctgg
     7381 atatatcgtg ccttgtccca accattttac atttttcaat ttctctgctt cctcttggaa
     7441 tacatcaata aatactttac cgataatttt tcgtttcgct tctggctcag caatgccttt
     7501 gagctcctca taaaaacgag ctgaagcatc aacacctact acatttaaac ctaaatgctg
     7561 ataagaatcc aatacagatt gatactcatt ataacgcaat aaaccattat tgacaaatat
     7621 acaaactaac tgattgccaa ttgctttgtg taataatagt gcagcaacac ttgaatcaac
     7681 accaccactc aaaccaagga caacatggtc gctaccaacg gtttgtttga tttccgtaac
     7741 ggtttcctca ataaatgaaa gtggcgtcca atcttgtcgg cagccacaaa tatcgaccac
     7801 aaaattttgc aataaagttt ttccgtcagt tgaatgcgtc acttcagggt ggaattgaat
     7861 accatatatt tctttttcgc tgtgcttaaa tgcagcgaca ggaatagagt ctgtatttgc
     7921 taaaagctca aattgtgctg gcaatgctaa aacagaatcg ccgtgactca tccaaacttg
     7981 agattttgtt tgaatattat tgaaaaaagc agaatcggct gcaataaagt ctaggtgtgc
     8041 tctgccgtat tctcgttttg aagaacgttc tacttttcct ccaaaaacat gtgcaatata
     8101 ttgagcacca taacaaacgc ctaaaacggg cacttgatat gttgcttgca cttcttttat
     8161 tggaaattgt aaggcatcgt cttgtaaaac agaaaatggc gaaccagata agattatgcc
     8221 attaaaatct tttttctcta aaacagctaa gtggttgtaa ggatgaattt cgcaataaac
     8281 gtttagctca cgtattcttc tagcaattaa ttgtgtgtat tgtgaaccga aatcgaaaat
     8341 tagtattttg gaatgcatgt gcaaaggtac atgacagaat gcaaaaggca aaagaaatca
     8401 agctttttat ctgcaaattc attttatcat aaaataaaag gcaataatat gctattgcct
     8461 ttctatacat cgaaaaatgt catttttagt ttaagctacc aaaatctaca ggaacaacac
     8521 gagaaattcc ttgttttacc atggttacac catagataga atctacactt gccatagtag
     8581 atttgttatg tgtaaccaaa ataaactgcg agttttgcga gaacttgcga atggcttcat
     8641 tgaatttgcg cacatttgca tcatcaagtg gtgcatctac ctcatctaaa atacaaaacg
     8701 gagcaggttt gtacaggtac aaagcaaata aaagtgaaag cgccgttaat gttttctcgc
     8761 caccagaaag ctggttgatg ccttgtggtc gcttaccttt tggcttggca ataatatcaa
     8821 tttccgattc taatggatca tcaggattca ctaacactaa atcacaatta tcgtcttcgg
     8881 taaacatact cctaaacaca gaaataaagt tttcgcgaac ggcattaaaa gtttctaaaa
     8941 actgaccttt tgcggtattc tcgatttcga gcattgtttg cattaggttt tctttcgaat
     9001 ccaataaatc ttgtttttgt gtggtaatga aatcaaaacg ctctttcatt tcattatatg
     9061 cttcttctgc cattggattt acctcaccaa agttttcaat acgacgtttg attttttcta
     9121 atctgtcaaa taaatcatct tttggcgttt ctggcaagct taaattattg gcaaattctt
     9181 caacttcaat atgaaattcg atagaaagtc gttctttcag tacatttaat tttaacttta
     9241 attcgctgag caattctttt ttagacatta tattttgctc aattgcttgt ctgtttttat
     9301 ttttttcgcg aatagcatct tctatacttg ttattccttc ttttactttg taataatcgg
     9361 tttcttgcgt agaaagcgat tgcatttgca attctttttc tttgtacgat tccattaata
     9421 ccgtttcaag tgcatcgatg gattcagaaa gtatatcgat ttcaagttgt gagttttcga
     9481 tttctttcac tgcattttca agcctatttt ggttttcttc tagttgatgt tgcttatatg
     9541 taagcgtttg ttggttcgat tttaatttat tttcttgttg tataaataaa atattagctt
     9601 gattaaatgc ttgcgaaaac tgattgtata attcgttggc ttttctaaag ttgatttcta
     9661 aattatctaa ttcacttttg taagtatttg cagtgttaat atgctcttct aatgtagttt
     9721 ttaatggttc catttccaat tgcaaagcat caatttgttg ttgaagtgct tgtgcttgcg
     9781 agcttatttt tcctatcgaa ctttcttcac tttctatttt tgattttttg gctacaagtt
     9841 ctttttctat ctgttgtaat tcatgcgatt cgcgttcgat gacatttcta aatgagttta
     9901 cttttaaatg ttgtaattcg ttgtaattgt cttgcgttac ttttttgaat tgtaatactt
     9961 ctgtttctaa ttctttgatt tctttttcta aaatctctaa attttttaaa cgacctaatc
    10021 gttttccttc aaataaacca acggcaccac ctgaaatttg tttatctgat ttcaataaat
    10081 gcccagaatt agaaatgata aaaacatctt tcttttgtgc aacattatca aatatatggt
    10141 tgacatctgc gccttcatct gcgatataaa caccatgtag caaatacgct aaaatgggtt
    10201 gatatttttc gtcaatttga atgatatcaa tggctttttt agcactttgt atgttgttat
    10261 gattatcaga aatgacagct tttttgaaat agtctaaaat taaaaaactg gcttttccta
    10321 cggaactttg cgataaaata tcgatagcgt tgatggcttc attttcgctg tccaccacaa
    10381 aattattcaa atagggttga agtacacttt cgatggctac tctatatttt tcttccgaat
    10441 aaataatatc agaaagcaaa ggtgtgtctt ttaaccaatt cacatttttt ttcaaaaatt
    10501 taattgactc aggaaatcct tccaaattgt caatcatatt tttagttaat ttatattcat
    10561 tgtttttggc gtctaaaatt ctgtttttat tggttaactc ttgtctgagt tgctcatttt
    10621 tttcttcgag tgtttttatt ttctcgatat tttcttcttc tttatcacgt aagtcatcaa
    10681 ccaatgcttt ttgtttttca attttcgatt gtgtattgct gatgtctgtg tgtagtgctt
    10741 ctaaagtttt tactcgttct tcattttcaa aaatagattg ctgtgcacta cgttgtaatg
    10801 attcgtattg cgtttcttta acggcaatta gtttttctgt ttccgttatt ttattagcac
    10861 agctattgtg tttatttctg gcttcgtcta aattatttct aaactctaaa tttgtatttc
    10921 tttgttcttc tagtttttgt ttagcatcgt caaattctac acgaacaatt tcgagtttgt
    10981 gctcgtcttc ttcattgtaa tttttcaagt ttgcaatttc agatgacaag ctttcgataa
    11041 tcgttttcgc ttgattgatt tgttgattta attgaacgat ttgatttttt aaaaagcttg
    11101 atttttgttg gtcaattttt ttctgacttt ctttttcttg tagctgagac aagaacgagt
    11161 taagttgttt ttgttggtct gataatgatt tttccttatc taacactgcc aatttttctt
    11221 tttctaagtt ggcttctaaa acttctattt gtgtatttgc ctctaaacgc ttgtcttctt
    11281 gctcttcgat ttctttatta gacaataaga aactttcatt gataccatgt aattcatatt
    11341 ttgacaaatc gatggaaacg accttatatt cttctttgaa atttttatat tgtcgcgctt
    11401 tttttgcttg cgattctaat gattttaagt ttttatcaat ttcaaataat aaatcttcta
    11461 cacgattcaa gtcaccttct gtagcttgta gttttgctaa tgtttctttc ttccttgttt
    11521 tgtatttcga tataccagcg gcttgttcta tgagcgttct tcttgcatta tctacgttat
    11581 ttaaaatctg atctatcatt tttagttcaa tgatagcata cgtatctgta gagataccag
    11641 tgtccataaa taaatcgcga atatctttta aacggcacga tacaccattg agtttataat
    11701 cgctgtcgcc atcgcgctca acggtacgag taatagtaac gctcgaaaat tctgttggca
    11761 ataaatttct agtattatct aatgtgattg aaacctcagc aatatttgac gcagatttat
    11821 ctttagtgcc gttaaaaatg atgttatcca tcttttctaa acgcaatgct tttgttttct
    11881 gctcacctaa aacccaacga attgcatcaa caacatttga ttttccacag ccatttgggc
    11941 caacaattcc agtgatattt ccgtcaaaat taatttgtgt tttatcggca aaacttttaa
    12001 atcctttaat atccaaactt ctcagtctca tcgtttctgt ttttttctaa aattatggaa
    12061 aatccataaa cggcaaatat acacgaaatc aagcaagaga tttagcggaa atcagctttt
    12121 ttatagacat tttaagcgta tttcttggta aaatcgtgga aagaatgtgt ataaatttat
    12181 agagtaatac ttttaattac cttataaatt atttgatttt ctgtttgtta tataaatatg
    12241 acatttatct caaacgacgt ttcagcgttt cgctgatatt tttcattcta tttagattca
    12301 tcagtagtgc taacataatt tttatgggaa aactaatcag ataaaatgct atataaaata
    12361 tatttggttt tatgttttct atttcataat tatttttcaa taaattgctt ctgcacattt
    12421 tttgacatat ccagcgttca ctttcattta aaccgccttt tttccaatta gaagcacgtt
    12481 cttttttaaa tccaatttct ttgctatctt tttcaatcga tgagctttct tgtgtaatat
    12541 tcaacatatt gctatcaaaa gcaacttcga tgtgattaca aatagaccgt aaagttgttt
    12601 caggcgcttc taataaatct tcgaaacgaa taaacataac acgtttatct tgcttgaata
    12661 gcttggctgc atgtattgcc gcattccaca atttacttaa tgtaattgga tgataattga
    12721 tacgtaagcg taatgcttct tttcgtgtca tataatcgcc gcctaagttt ctgcgattcc
    12781 atttattttt ttgcgataat aaaatggctc ttggatcgcg caccatatta atgaccttag
    12841 catttggata tagctcaaaa atttccgaaa gataaaaaac attttgtggc gttttttcgc
    12901 aaatgatagt tttgccatgt agggcaactt cgcgttgcat aaagtagtga aaaacgtctt
    12961 ctgctgttac atctggtttg ttgatttcat taataagtaa tgtcgcttcc tcattaaatt
    13021 gtgaaaagtt ttggtcgtgt gtcatataac caattcgctg tgtcaatagc aattttgcag
    13081 caagcgtaat agcaccttct ttagtaataa tttttccttt gtcttctggc gaccataatt
    13141 gctcaaaaaa atgtaattca ttcaacacca ttaattgtgg atgcatatta aaaatacgca
    13201 acatcatcgt ggtgccactt cgtgaatttc cgacaataaa aattagttta tcttgaaacg
    13261 acaatttttg tagatttatg caaaagtaag taatcgcaat gagtagttcg tatctgcatt
    13321 actatttttt ttatgattta aaattagata atcaaagatg aattatcata taaacacaaa
    13381 atatacaaag tcgatttctt cctgtcagca gcattgtttt attgtagtaa gcttgttttt
    13441 tatgttatta tatcataatg tcaatgctca aaaaattatt gctggcccta ttattggtgc
    13501 cgtaagtgat agctcagcga aattagtgtt tatgacggac aagcccattc atgctactat
    13561 caaatgtatt tcaactgctg atacattaat tcaaacgcat cgcacatcaa ctgatgatgt
    13621 atattatgta aatgcctgcc aaattaattc caaattaaaa tcaaatacaa attatgaaat
    13681 acaaataaat attactggaa acgacagtac ttacatatct tcatttaaaa cttttccttc
    13741 aactaaagag acttctttat tttcatttac tttaggctca tgcacggagc aacattataa
    13801 tgattctatt ttttttgaaa tgaaaaagca caagccattg ttttttttac aaattggaga
    13861 ttggttatat gcgaataatt ttaacgaacg aaaattttat tatgccgaaa gtatagcgca
    13921 tcaaaaagag ttgtatgaga agcgatatga aatgccaaat ttaaagaaca tgcttgcttc
    13981 aactccaata gattttatgt ttgacgatga agacgctgtg tttgatgatt tttcaaaaaa
    14041 tacatatact catgttcagc aagttgctaa tgcaactcaa atttctgaga tagcttatcc
    14101 agattcattg cgtaaaaatg ttatagaagg ataccatact ttttttccgt attacaatca
    14161 tttgcccaat caagcatacc attcattcgt atgtggcaat gcagaatttt ttgttgtaga
    14221 tactcgttca acacgcgatg caaatacagc ttcttttgtc aaaaagaata agcattatgc
    14281 ttataaggtg ccaaatcagc atttattatt agatacttta cagtggaatt ggctgctgtc
    14341 gtctttaaaa aaatcacagg cacaatggaa atttattgtg agtggcgtta cttttaataa
    14401 atcatataaa gacgtgtttg atattgccct gttaaaacct gcgcaaaaca agaagctagc
    14461 caatggtatg actggtttat ctattgcagc aggattatct accatgtggt ttgcttttcc
    14521 agaaacacaa atagcgttac ttaatttttg tcatgataat caaatcaaaa atgtaattgt
    14581 tttaagtggt gatgcacata ctgcagcgat tgatgatggc gcaaatgctg gctttcccga
    14641 actgatggca ggcggattgg cacagaaaaa ttcgaggctg gctgcaataa ttaagaatca
    14701 attgcaccta aatctatgga atcaaggtgg gcaaggtatt ggtaataata attttaatga
    14761 tgcatttgga aaagtggata ttaatggcaa tcaatctgtt gttttatctt gtattgataa
    14821 atatggaaaa actatttgtt cgtatgaagt caaagatgga tttattccca aaaaatacac
    14881 attaaaacca atgctcagca tcaataaaat cagggctttt aaacatgcat tacgtattaa
    14941 attgaaataa ttttaccacc tattaaatac atcttctgca aaacctaaga cgcgttgtac
    15001 ttgaagaatt gtaccatcat ctaaaacaac acgtccagaa aaatagccaa atacttgatg
    15061 ttgcacagac tttatcaata tgagattagt attagaatat ctgtctattg caggttcaaa
    15121 atcaagttca aaccgcttgt cattactgct aaacttccat ggtttcaaat aatttgaagt
    15181 atccatatga aaaactactt gatctaattt atgtgcaaca ccatcataaa acaacatatt
    15241 ttctgttgcc gcacttgtgt taccaaaacc ataaccaata ttaaaaccaa aaggcttacc
    15301 attgacataa gcagaagcag aaccccaata ccatctattt tgatacgtcc aaactccgcg
    15361 tccccaatct agtactgctg ttgccgtttt tggattaaga tgatattgat tgccattata
    15421 ggtaaacgaa ccacttgcag ctaaacaatt aattttttgg ttgtagtaaa atgctctttt
    15481 attttctata aaaggcgttg ctataaccat gctgtcttta tttattgtta gcagcttaat
    15541 acttgcttgc aaacctttac cttttgcaaa tccaggatat tgtatttcaa tgttcctatt
    15601 tccatctttt atattgattg cgatatttat atttttattt ttaatgatga tattgccaac
    15661 agatgattgc gctggaagtt gcatttttcc taaaggaaaa ggtattaaca cttggtcact
    15721 tatttctgta ttgtttgtaa aatcaaataa attcacagcc aatacgccaa cgtatgctaa
    15781 atcagcaaca gtaatcgcaa tgccatattt atcttgtaag atgcaataat aatcccattc
    15841 ttttatgctg tactttgacg tttttatatc acttctgtta taagttaata tcaaatcacg
    15901 agcccatcct ttttgtatta atatgccatt tttgtctaat aaatttccag cttgtacaat
    15961 ttcattttgc atataaaata tcttttgtta tttgttaaag ttaacgaata ctactacatt
    16021 tattgactaa aatacgaaaa tgagcaatct gaatgtaaaa catgaatata tagaagtaaa
    16081 cggaataaag ctccatgttg cgaaacaagg caatggaaaa aaactagtgg ttttattgca
    16141 cggatggcca gaattttggt acacttggcg ttatcaaatt cccgaattag ccaaacattt
    16201 cactgtgtat gcacccgatt tacgcggttt taatttgagc gataaaccac atggaatttc
    16261 aaattataaa acagatactg tagccagcga tattgctgca ttggtacaac aatcaggatt
    16321 taaaaaagca cttattgtag gccatgattg gggcggagct gtagcttgga cattcgcgac
    16381 gatgtttcct gaattgactg aaaaattagt ggtttgtaat tgtccgcatc caaaattaat
    16441 gttagatagt tttaaatcaa atccatcaca gttaataaaa agctggtata tttttatgca
    16501 tcaaattcca ttattgccag aaatgattta ccaatttaca ctacctttat ttttcaaaca
    16561 atttgtgcga ggttggatgt ataataaaca caattttagt gatgatgatt tgaatgaatt
    16621 tgttaaagca tttaaacacg aaggtgcatt aacaggctca ataaattatt acagagcaat
    16681 gttgcaaacg aaacctaatt tgaaaatctt tgataagaaa atccaatcac caaccttatt
    16741 aatttggggc gaaggcgata aagcattagg caaagaatta acctacaaca cagaaaaata
    16801 tatcgacgcg gcgtatgaag tgaaatatat tccaaattgt tcgcactgga cacaaaacga
    16861 ttgtcctgaa ttagtaaatg aattcttatt agattttttt attactaaaa aataagattt
    16921 tttaatttta attttattta aaactcaatg tgttttagcg tttttactca ctatatatag
    16981 tggtttttca tgcattttac tgctttccaa taaaaaatta ttgacattta caacgattct
    17041 taataaaatt gagtcttaaa cataaatgct ctataattca cccatattaa aatcaccaaa
    17101 gtggctttac acaaagcatc tgcaaactat tattccaaat gtatatagaa aagtggaagg
    17161 tgtgttctat cagcgtgagc gcatagaaac ttgggacgat gattttttgg atttggattg
    17221 gagtaaagta aacagcaata aaattattat catttctcat ggctttgaag gcagctctga
    17281 tcgtgtgtat gctagaggtt taacaaaaat actgaatgca aatggttttg acgtatgtgt
    17341 gtggaatttc agaggttgta gtggagaaga taatcgtcag ttttttggat atcattccgg
    17401 aaaaacagat gatttagatt atgtagtcaa acatatattg acaacaaaaa actacgatga
    17461 acattatatg attggcattt caatgggtgg caatcttacg atgaaatatt taggtgaaga
    17521 caaatgggaa acagcaaaaa aaattgtgtc agcgcttaca gtgtctgttc cgattcatta
    17581 cgaaacatca tttgagcatt tgtgtaaagg ttggaattta atatacgaac gtcgatttac
    17641 taaacaagta aaagataaag tgctcaaaaa aattacaaaa tatcctgatg caatcgacta
    17701 caaatatgta ttggaagcaa agaatttatg ggaatttacg gctcgttgca cagtgccaat
    17761 gcatggtttt actgacattc atgattataa taaaaaagtg caagcatcta cctacataag
    17821 aaatatacaa acgccaatat tgcttattaa tgcagacaat gatcctttgt tgaccaaaga
    17881 atgttatccg acacaagttg cgaaaaaatc aaattttttc catttagaaa ttacaattgg
    17941 cggcggtcat gttggctttt gtgaagatat aaaagcagaa cataattggt tagaaaatcg
    18001 tgtggtaact tattttacaa aaggctacta agcctattaa ttattgtctt cttcgtcttt
    18061 ccaagggcaa tgtttgcagt tatttccaca acaataacct cgcttttgat gatatgcttt
    18121 tgtaaataca taattgccat tttcatcgat gtaataatca atgtttacca ctaattcgtc
    18181 cattattttt gttctttatg taaaagataa gctttgaaaa atccattcaa atcgccatct
    18241 aatactttat caggattggt gactttatat tcagtacgta aatcttttac ccaacgatca
    18301 tctaatacat aacttctaat ttgcgagccc cattcatttt tcattttttg agcttcaatg
    18361 gcatcgcgtg cttctaattg tttttgcaat tccgcttcat acaatttaga tttcaacatt
    18421 tttaatgctg tttctcgatt ttgtatttgt gagcgttgtg tttgacattc gcaaacaata
    18481 ccactcggca agtgacggac acgtacagca gattccgttt tgttgacgtg ctgtccgcct
    18541 gcaccttgcg ctctaaacac gtcccactct aagtcagcag gattgatttc aatttcaata
    18601 gagtcatcga cgacaggatg aacgaatact gagcaaaaag aagtcatgcg tttgccttgt
    18661 gcgttaaatg gcgagatgcg caccaatctg tgtacgccat tttcaccttt taacatgccg
    18721 aatgcaaaat cgccagcaat ttctaatgct acagatttta ttcctgccac atcgccagct
    18781 tggtaatcta gttcagtaat tttatagcca tttttgttag cccacatcgt gtacatacgc
    18841 atcaacatag aggcccaatc acaagcttcc gtgccaccag ctccagcatt tatttctata
    18901 attgcactta aattatcttc ttcattttta agcgtagatt tgaattcatt ttcatctaac
    18961 tcatcaataa ttgcttgata ttgcaagtct atttcttcgt cactggcttc accgatttct
    19021 tgaaattcac gcaatacatt taagtcgttg aattttgttt ctacactttt aaataaagtc
    19081 acccaatgtt tattttgttt aatttctttc atcaaagctt gcgctttgag ctggtcattc
    19141 caaaaatcag gtgctaaagt ttttgcttcg tcgtctttta tctgttcaat tttattagcg
    19201 acgtcaaaga tatcttccga acgaggcaac tcgttctaga agtagtttat attgttcgtt
    19261 agtcatgctg caaagatagc aaaaatcgcg tattaataaa aagcatcttc gaagtgagtt
    19321 atcatattgt ttttttcgat agcaatgata tcaaatctca attcgccttt ccaatcgtat
    19381 aattcaatat aagcatcagc tgcttctcgc attttcttaa ttttatgctt atcgacaaag
    19441 ctttctggcg taccaaacaa agatgagctt ctttttttca cttctacaaa tatgacgaca
    19501 cgttgatatt gtgcaatgat atcaatttct gcacgcttac tcctccaatt gctaaataat
    19561 atcgtataac ctttcttttg gagaaattct aaagctagtt tttcacctgc gtcgccaatt
    19621 tgattacttg taggcataat ttaaaattaa agatactaga ttttgagtta ccactatttt
    19681 tttgtttttt tacacactta aaatcaatca cattatataa aataacaaat gtgatatata
    19741 aacagatatt tatgaaaatg gacgatttaa ttcaaggatt tcctgcacaa ctcaaaagag
    19801 ctattgaaat aggcgaaagc gcaaaaattg gtaaaaataa atttccaatt agaaacatat
    19861 taatttctgg gcttggtggt tcaggaattg gcggcaccat cttagcaaat gtattacgcg
    19921 acgatgtggc tatgccaatt ttagtgaata aagaatatca aattcctgct tatgtaaatg
    19981 aaaatacact tgttattatt tcatcatatt caggaaatac agaagaaact ttatcggcat
    20041 taatgcaagc attcaaaaag aatgcgcaaa tcgtatgtat tacgtctggt ggtttagtac
    20101 aagagtatgc agaaactaac gatatcgatt atgtattaat agatggaggt gctccaccac
    20161 gatctgcttt tggacagtcg tttgtgcagc ttttgtatat tatgcatcat ttaggtttgc
    20221 tagaagatgg atttaaaaaa tatttgcaag aagcgattac gcttttagag agtgaagaag
    20281 cagacattaa agcacaagca aaaaatatag cacaaaaatt aaacggcaag atacctgtca
    20341 tttattctga tgcaaaattt gaaggcgttt gtattcgttt cagacaacaa ataaatgaaa
    20401 attcaaaaat gctttgttgg catcatgttg tcccagaaat gaaccataac gagttggttg
    20461 gctggcgcga aaaaaatgag caaatcgctg ttgtgttttt acgaaataac aaagattttg
    20521 agcgcaacca agaacgcatg gagtttacaa aagaagtagt aagtcaatat gctagtgatg
    20581 tggttgaaat ctattcaaaa ggcgattatg atttaatacg aagcatctat ttaatccatt
    20641 tgacagattg ggtttctttc tatttagccg aattgaaagg agttgatgcc attgaaataa
    20701 atgttatcaa taaattgaaa agtaaactag cagaaaatcc tttttagttt tcttaatttc
    20761 ttttttttat tgctatttga cttccatgaa ataacatgtt ttgacagcaa atgagcagga
    20821 tttataatac aattggttca ttaacaactt taaaattaaa attaaaggat aatcagatta
    20881 atgactttca ttcattaaat gaagtaatta actttcaaaa atcttttcat gcaattaaac
    20941 aagaaattct tattaaacat gaagatttta ttgagcgtga aaagaaatta cttgttaatg
    21001 atttgcaaaa tttagaatct gaaattatta cactagaaaa acaatataaa gatagatttg
    21061 ctttagaaat tggaggttta aaacaagaat tagttaatgt tgaaactatt ttatcaaata
    21121 attttatcca aaagattcgt tttcttatta aaaaatggat tttaaaaaga aaaattcgag
    21181 acaaagaatt tgactttaat agacaagttg aaatggatat taatttacgt attgaaaaac
    21241 ttaaccaaaa aaaatctcgt tttcaattta ttactttaaa tacaaagcaa gcagttgtac
    21301 taagtgcgca aaaagctata ttggaattag aaagaaagaa agcaattgta gattctttaa
    21361 atagttatat ttatggctca attggtgaaa ataaagtagt caaaacctta gataaattat
    21421 ctgatgaata ttacttgatt aatgattttt cgtttacgtt ttctcctgct atatattata
    21481 accaagaaga tgagtatata aagtcaatac aattagatca catactaatt ggaccatctg
    21541 gtgttttttt aatagaaaca aaaaattgga gcgagaaatc tattgataat ttagatcttc
    21601 gctcaccagt ttgccaaatt agacgttcta gttttgcact ttttaaatta ttaaattctg
    21661 aatcagatga tttaggatta caattaaatt cacatcattg gggaagcgta aaaatttcaa
    21721 taaaaaatct tattgtacta ttaaattcaa agcccaaagg tgagtttcaa tatgtaaaaa
    21781 ttttaacttt aaacgagttg ataggctata tttcttattt taaacctatt tttacacatt
    21841 ctgaagttga atctgttttt aaatatattt acagattaaa cgaacaaaac taatacatat
    21901 agatgtgctg atttaatagt attaattcta tttttcaatt tttagacatt catcaccatt
    21961 ataattgtca aactaaaaca gccatgcaga aaattagagt tcatgataaa ggtgtgatac
    22021 aatatgctga tgcattagta ttacaaactg aaaaatttga tgaacttatt gaaaataaaa
    22081 ttaataatat aactaacaaa gatgcgcatc atcttttttt atgcgagcat caagctgtaa
    22141 ttacattagg caaatcagca aaagaacaaa atatactatt gcctgaagca ttactcaaag
    22201 aaaataatat cgatatattt catatcaacc gcggtggtga tgttaccttt catggcccag
    22261 gacaaattac aggctatcca attttagatt tagatttttt tacttcagat atcaaaatgt
    22321 atatgcgtat gttggaagaa gtaatgataa gaacg
//
LOCUS       DAOQQV010000031        21876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_711_length_21876_cov_7.541084, whole genome shotgun sequence.
ACCESSION   DAOQQV010000031 DAOQQV010000000
VERSION     DAOQQV010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21876
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_711_length_21876_cov_7.541084"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLL59_05015"
     CDS             <1..262
                     /locus_tag="PLL59_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23199.1"
                     /translation="QHITPDNPLVTERINAMKAGLLSVTTDADKATHQALEFLNITVN
                     RQAAILSYIDIFHYLTLFIVVSVPLVLFAKTYKINKDMMEGH"
     gene            complement(270..1397)
                     /locus_tag="PLL59_05020"
     CDS             complement(270..1397)
                     /locus_tag="PLL59_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23200.1"
                     /translation="MRPIYILFIVFTAISGLTQCKKDKNIDCIDNTQRNELLQSLQKY
                     SYDSVQFSGNERRFLNEIRAIANDIDALSDNVDTTISIENTYDQILSNFLAHIALVEQ
                     KANAFYNGSPAKIVLTKNAFVLMSYDALQRKYPEFMWTQLGIFAANEVRNGLVMALML
                     RQTLMKNNINIPFNGSDVSSVLLATSDVLIQGQLDVLSDIGCLGILNKKIGAGNIKNE
                     AWLTNEGREAFRLQELAEQQLAIGNCSQYMDLQTQAAIQFGAHEQIYILQPMWDQPLM
                     AQFALLNQVVTQLTQHQLVFFGDIFVGTNKSSEFNKGYTIKTPANVSNLANASQRVDV
                     AINGFNTINSIRKSKAGSYWIDYSQIKIGYFYDVYNAPWIE"
     gene            1511..1756
                     /gene="purS"
                     /locus_tag="PLL59_05025"
     CDS             1511..1756
                     /gene="purS"
                     /locus_tag="PLL59_05025"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HMY23201.1"
                     /translation="MIYTAEINVMPLKELLDPQGKAVEQSLHNLGFQTSNVRIGKHIT
                     LKIEAASKEEASAKVEEACKKLLHNPVMEYFEFVVSE"
     gene            complement(1760..3013)
                     /locus_tag="PLL59_05030"
     CDS             complement(1760..3013)
                     /locus_tag="PLL59_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23202.1"
                     /translation="MKKIIFCILLGISTYAKASFILIPMDESQSNHLKAYGIAYWILS
                     KNVPIDWLLNYRGGSFSFPYNKEFEKECLVRNVKYETIADATMSKIMDDIANPEINMD
                     AVRLEKAPKVAVYSPKTNNPWDDAVTLVLSYAEIPYTVIYDEEILDGNLHLYDWLHLH
                     HEDFTGQYGKFYAAYQNADWYKKQVADYETRAAALGFKKVSIEKLAVAQKIRDFVLGG
                     GFMFAMCSATDSYDIALAAAGVDICATPFDHDPMDPNAQEKLDFSKGFAFQNYTISSN
                     PYEYEFSTIDVPGVRPFSKDMDYFTLFDFSAKWDQVPAMLTQNHEKLIKGFMGQTTAY
                     KKQFIKSDVLVMGENKSLNEAKYIHGVYGKGTWTFYGGHDPEDYQHRVGDPVTDLNLH
                     PNSAGYRLILNNILFPAAKKKPQKT"
     gene            complement(3070..4581)
                     /locus_tag="PLL59_05035"
     CDS             complement(3070..4581)
                     /locus_tag="PLL59_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23203.1"
                     /translation="MRLLILLFCCTILFSCKKEKPIAETCTVEINQRLKNPNELSSEI
                     SLDKKIATHHYVKFHIYNIQQYHALEKQGIILLDHPFDAIPDKNLQYETAHTKQYGIY
                     YGVVPKSVNIDNYEAEILSALYMPETGNTMRTSETPSKQFAGSIKFFDPIDSLLKPLK
                     GVQIIIKEHTKMAIGFTDESGNFSFESQAITADTAEVLLKFDNDYLQIHTLDAANLFG
                     IFGVNTYSLGFRKSCAFTDLQIEIGRTFNNAALHHSCAALLALNSFKKFASNSSFLMP
                     TKKFNFWLGKEAPISTSYATPMLHNMTQQQIANPTRLITNLFGVSEDIAELLSYIIGD
                     QLPDIYAPYYSIYATTARASFIETMYHELAHASHFAKVGTDFWLPYVEYIYTNGGYGE
                     PTFSNSGIIGLSEAWAEDVSNIGLFYTYQKQKYINFNEHPFEDWIPYGLYYDLYDTGN
                     NEIFDNVSAITFPEMYSLLTTDTRSLQALKIKLKNNYPSQQAAIDLLFQHYGF"
     gene            complement(4667..5935)
                     /gene="icd"
                     /locus_tag="PLL59_05040"
     CDS             complement(4667..5935)
                     /gene="icd"
                     /locus_tag="PLL59_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HMY23204.1"
                     /translation="MSAEKITMKDGHLQVPNEPIIPFIEGDGTGPDIWRASVRVLDAA
                     VEKAYNGTRKIHWKEVLAGEKAYKQTSSWLPDETLTVIEEHLIAIKGPLTTPVGGGIR
                     SLNVALRQLLDLYACVRPVRYFDGVPSNSKVPEQVDMVIFRENTEDIYAGIEWLQGTD
                     DCKKLLHFLQTEMNVGDKIRFPETSSLGIKPVSLDGSERLIRSAINFALAHKRKSVTL
                     VHKGNIMKFTEGKFKEWGYKLAEREYGDKVFTWAQYDKILAEEGPEEANEAQKNAEAA
                     GKLIIKDAIADNFLQQIILRPSEYDVVATLNLNGDYISDALAALVGGIGIAPGANINY
                     LTGMAIFEATHGTAPKYANQDKVNPSSVILSGVMMLEFMGWQEAADLIIKGMETAITK
                     KTVTYDFERLMEGATLLKCSEFGDAIIKNF"
     gene            6135..7001
                     /locus_tag="PLL59_05045"
     CDS             6135..7001
                     /locus_tag="PLL59_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HMY23205.1"
                     /translation="MKKLVFNLFFLLYLPLSLFAQEIKIMSWNIFMVPPFIFKSCQTE
                     RAHLQAAFIKKNDPDIVVLNEAFMKSTRTILLEELQHIFPYQTKITKKGFFKTNSGVW
                     ILSKYPISNQQFMKYKNKKGSDIFSKKGAVYMEVTLPHNKLQIIATHTQSLAKYRTAR
                     EKQFTQLKTKLADVHFTESIPQFIIGDLNCDFYDTSAYQSMLQKLDVLPFHFTGEKYS
                     WNGKENELAYSFFDTIQESLDYILLRNAHKEKASVISTTIMNPKQDSCFCKNKYFNIS
                     DHHPIISIIQLK"
     gene            7079..8104
                     /gene="ruvB"
                     /locus_tag="PLL59_05050"
     CDS             7079..8104
                     /gene="ruvB"
                     /locus_tag="PLL59_05050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HMY23206.1"
                     /translation="MQNPNLDASGDYLSKTEKELEKVLRPQAFEQFAGQPQIIENLSI
                     FIQAAKQRDEALDHVLLHGPPGLGKTTLSSIIANELGVELKTTSGPVLEKPGDLAGLL
                     TNLQANDVLFIDEIHRLNTTVEEYLYSAMEDYKIDIMIDSGPSARSIQITLNPFTLIG
                     ATTRAGLLTSPLRARFGINFRLEYYNVDVLKKIIKRSARILNTEIDEKGATEIAKRSR
                     GTPRIANALLRRVRDFAQIKGNGIVDLEISKYALSQLNVDEHGLDEMDNKILSVIIDK
                     FAGGPVGINTIATAVGEESGTIEEVYEPFLIQEGYLKRTPRGREATAKAYTHIGKVPP
                     QQTGSLF"
     gene            8115..8321
                     /locus_tag="PLL59_05055"
     CDS             8115..8321
                     /locus_tag="PLL59_05055"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HMY23207.1"
                     /translation="MNDKLVKIATYFNQFDVDLAKFKLEENGIYCFIKNEHVAQLQFG
                     LANFELMVFQKDKEAALIILEQPV"
     gene            complement(8343..10409)
                     /locus_tag="PLL59_05060"
     CDS             complement(8343..10409)
                     /locus_tag="PLL59_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23208.1"
                     /translation="MPYEVKYNSKSNTIDSLTEENILVLNDFPVVVKEMPISYNTSVV
                     NERLFTAIQKSKGVITANDNNSDDVSQKSFFPSTDSIAGIYYRVDASKGKKQFGLGIS
                     NNGIYYKHKKQTYYLTITTGNTRNKLLIFRVDKNKLSYVNQFAIPGNGFQNGIDKKRH
                     YLKNPESAYLNLSNELPLTMLNTGHLLFPINIKEGNLKGIMSIDLNTFLSQYPYNEKV
                     ENKEAFVKVYGSRLDETKTTQQEYVQAIAPFDVTKVWISTNTAKVIALDIRYPSHAFL
                     YDMNSDTLPIIKEKRDILIDQLKESYDALYLNNKLPTGEQTFNYFSTTDWKQIKSTPA
                     NVKNYKKCASLLCKNNTKKCTYYPRIDMEKNKFVHEYLLKQLYAGKNKLQLLNPRDSA
                     NQKHQIQQAITVDENGDAYIVTNYEIAKFTYQQRAGQITQVWSKRYKNSFVKYPGSSN
                     VFNGTAATVFSNYVAITDQGFPNVSLSVYNKTNGKLLSSQILFEGTYSRCDNDVVVHS
                     SKAENDSTLHTLIISNNFGNAYPLDEVSYTQGGIEKYYFSENNSNSKQFEKLVADKKF
                     NQTALSNLVYAKTATPLLNTMNNEVVYIYNQQQATMASNSYANCQLQAIDFATGNPIY
                     NITPKVEKNAFTEYLDKKSRKKVSKNNYTQAIFNNIHGNMSQTPKGDFIVPVVRGFIY
                     LPSQAK"
     gene            complement(10527..11270)
                     /locus_tag="PLL59_05065"
     CDS             complement(10527..11270)
                     /locus_tag="PLL59_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMY23209.1"
                     /translation="MVKHIPNSLTLLNLFSGCCGIVACLQQEYTLVPIFLSISLLADF
                     LDGFVARALNVKSELGGQLDSLADMVTFGVLPGIMLFNIIQAKTNFITTIAASSPINL
                     LEQPVALVGFVYTLFACLRLAKFNLDTRQTENFIGLNTPSGALFILGFYMQFFSDWSL
                     QSNWDFTIYAKIAAIIIPFVLAFLMIAEFPMFSLKGNPFSWKQNKFRLMLILSVIPQI
                     LLLKWLGLSTIILTYIIFAFIQNVNKNKK"
     gene            complement(11314..12756)
                     /locus_tag="PLL59_05070"
     CDS             complement(11314..12756)
                     /locus_tag="PLL59_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMY23210.1"
                     /translation="MSTLLPTTGLDRDEIHQQWEAYTHDDMNWKEGKFFGYVYYPGDD
                     YYKIIKEAYATFSATNALNPAVFPSLKKMENEVVRITASLLHGDEQVSGCMTSGGTES
                     IYMAVRAAKEWSKKNKTTKNPLMLLAETAHPAFCKAADAMELNYKLIKLNADFQLDIE
                     DLKKNLSTDVSFIVASAPSYPQGAIDDIEAIAKIAKQHKCLLHVDACVGGFILPFLKM
                     EEVDLPKFDFEIDGVTSMSADVHKYGYAAKGASVVLYKNANLRKGQFYVKTNWSGGIY
                     GSPSFMGTRGGGPIAAAWTALHHIGEEGYKRMARETFEETVFVKNKIKEELPELKILG
                     NPKATLFAFSSDVIDVYEVADELALLGWTINRQQLPASLHVLLNYIHVGKGELFINDL
                     KTAVKKAKQFSLLHLKNNIQLSAVKGLKKLLNEQQFSTLTQKFSGTEDGGKRKAAMYG
                     MMSELSGSGTLNDLVLDYLDKLYQIDDKNA"
     gene            12895..14430
                     /locus_tag="PLL59_05075"
     CDS             12895..14430
                     /locus_tag="PLL59_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HMY23211.1"
                     /translation="MLVKTFGSAVYGVDAATITIEVNICQGQHYFMVGLPDNAVKESW
                     SRVESALRVNKYHMPRIRIIVNLAPADIRKEGSAYDLPIAIGILGASEQINTDKLADY
                     IIMGELSLDGKLQPIKGALPIAIQAQKEGYKGFILPKENAREAAIVEGIHVFGVEHID
                     EVVEFLDNKKELEPTVVFVEDDFNNSLQNNLFDFADVKGQENIKRALEIAAAGGHNVI
                     LIGPPGAGKTMLAKRMPTILPPLSLQEALETTKIHSVAGKLKCNSALLYQRPFRSPHH
                     TVSNAALVGGGSNPQPGEISLAHNGVLFLDELPEFKRDVLEVMRQPMEERKVNISRTR
                     FSIDYPANFMLVASMNPCPCGYYNHPEKDCVCAPGVVHKYLNKISGPLLDRIDLHVEV
                     TPVKLEDLSSKTVYEKSNAIRKRVIAARQIQEARFSADEIHCNAQMSTNLTQTWCGLD
                     NTSQNLLNAAMKRLGLSARAYERIIRVARTIADLDSSEKICNEHIAEAIQFRSLDRES
                     WSA"
     gene            14599..15741
                     /locus_tag="PLL59_05080"
     CDS             14599..15741
                     /locus_tag="PLL59_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HMY23212.1"
                     /translation="MLSDFLQPIQQQLLDNYYRPNKYANAYIHLYQEKQTLENYSIAL
                     LGVQDYRGYIANKGTEHAADEVRKQLYQFSAFTNGVQLLDLGNIQAGETYVDTLVALV
                     ETLHELFKRNIITIILGGDAALASAQFKAYEIFENPITVSHVASNVLWDNSDMDIHKT
                     YLKDFFLSNFLKKYNLVAYQSYFMHEKQLSFLSSKDHDVLRVGKIREHVFEAEPHLRE
                     AAMVAFNINAIRYSDAPAQPEPSPNGMFGDEACAVARYAGMSDQLMSIGFYDYNPTLD
                     KSNITAKQVAQMLWYFMEGISLRRHDYPIVNENDFSQYIVQIEDAENDFIFLKSRKSD
                     RWWMKIMYDKKDLQKHKLIPCTYKDYLTALNNEIPERWMRAWMRLS"
     gene            15849..18716
                     /locus_tag="PLL59_05085"
     CDS             15849..18716
                     /locus_tag="PLL59_05085"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY23213.1"
                     /translation="MRRFVALLLFSFSFSILLANDFTVRGKIIDQKDHSPLIGVNIIL
                     SNTLDTLKTQGTSTDVEGDFSFALVDSGEYLLTVSYISYQTIQKNITIKNNVDLPEIA
                     MKEDSKLLKEVVIEDKQVRVQQLGDTSQYNANAFKVNSDASTEDLLQKMPGVTSENGT
                     VKVNGEEVKKILIDGRQFFGDDTRTAVQNIPADIVDKIQVFDKLSDQSQFTGFDDGNS
                     QKTINIITKNGITNSKFGKFYAGYGGPNNRYSAGLNYSAFKGDRRFTILAMSNNINQQ
                     NFNIQDLIGSMGSGNDRQGAGGRGGPGRNSSIGNFLVGQQNGIATTSALGLNYSNKIG
                     KQKKVTLSGSYFFNGSLNENNSASTRNYISSNDSGLVYKEDKDIDSKNFNNRLNFKIE
                     YAIDSSNALTFTPSISTQNYISTSNLLANNFSNTNHSSSNTENKQHSKSFGINFSNDI
                     LYQHKFNKKGRTISTNLNTVVNSKANTASLYALNTDRIDTLIATDTLDQKSTLYALSY
                     TIGGNIVYTEPIKKNGQIAINFTPSFTRSTSDKNTNNFDLASDSYSITDSILSNHFIN
                     KYITNKIGITYRYNDAKKLSWSIGISGQSALLKNEVIDFTATPVSRHFLSVLPNADLN
                     YKFSKKENLRFFYRTSNNPPSISQLQNVIDNSNSLILTSGNEMLKQNFSQTIGIRYSR
                     ANTAKATNFFLFTNATNTINYIANATTIFSRDTIIQGVKMNAGTQFIQPVNLNGYWNV
                     RTFLTYGFPITKMKSNMNVNAGFVYLRTPTLINMSKNIANAYTLNAGITLSSNISKKI
                     DFTLSYHANYNIVRNTLQRINNANYYNHTATARFNYQFWKGFVFNTSVSNTLNAGGSS
                     NYNTSYWLLNASLAYKFLKDESLEVKFTANDILNQNKNITRNVTEVYVEDIQSTALRR
                     YFMGTITYSLRKLGTGNADADKPKDFMLLPPPSGMPPPPGH"
     gene            complement(18717..20000)
                     /gene="murF"
                     /locus_tag="PLL59_05090"
     CDS             complement(18717..20000)
                     /gene="murF"
                     /locus_tag="PLL59_05090"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HMY23214.1"
                     /translation="MEIQELYKLFLQANGITTDTRKIEKNQIYFALKGEQFNGNSFAE
                     KSIELGASYAVIDEEQYAINEYCILVDDVLATLQQLANYHLHQVKPKAVIAITGSNGK
                     TTTKELVHAVLETSFRTHYTKGNLNNHIGIPLTLLEMKADTEIAVIEMGANHQQEIAN
                     YCAYVEPTHGIITNCGKAHMEGFGGIEGIQRGKGELYDYIKSTKGTIFVNGDDAILLK
                     MATTRDISSVITYGNSEDNKYHSIIEIDNPFLRLNFEGTIINSNLFGSYNYSNIMCAI
                     AVGKYFDIENQKIKHAIEQYHSTNARSQVIKKDSYELILDAYNANPTSMQHALESFTK
                     ATSKNKIVILGDMFELGEYTAEEHQKIANLATALHFDKIILVGKAFHATHTIDAVLKF
                     TNALEAQQWFRQQSFSDASILLKGSRSMKMEKVIE"
     gene            complement(20062..21570)
                     /locus_tag="PLL59_05095"
     CDS             complement(20062..21570)
                     /locus_tag="PLL59_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMY23215.1"
                     /translation="MTYKRMKNFLHLIIVASVLAVSCKGGNKGGGASSTTGWNYNDKK
                     WGGFEKSPNTQQITGPNLVLVEGGTFAMGATEDDLLYENHNVQRRVTVSSFYMDETEV
                     TNLDYNEYIYWLERVFGTDHPEFVKSAKPDSMCWRRNLAYNEPYVKYYFTHPAYNTYP
                     VVGVNWLQATEYCKWRTDRVNEMILVKEGYLDLNANQKNEDNFNTEAYAVGLYQGATK
                     KQKEDLNPNGSGKRNVNGKDGILLPDYRLPTEAEWEYAALGLRGNMPIPGEEVVTDRR
                     IYAWDGATFRYQRHNKNQGDFMANFMRGRGDYMGVAGALNDNAEITADVHTNFPNDFG
                     LFNMSGNVNEWTQDVYRPMTEADGDDLNTYRGNIFMKPSYDEGLDSMGRIKYVREEDE
                     DLSNRRNYRKSFALDYNDGDSSSQVEYQYGVSTLVNNKARVYKGGSWRDRGYYMSPGT
                     RRFLNEDQSSDDIGFRCAMVRVGSPDGNMFGGKGFGEMKKTISKNQKARKYK"
BASE COUNT         6985 a         3781 c         3723 g         7387 t
ORIGIN      
        1 acaacatatt acacctgata atcctttagt gacagaaaga attaatgcaa tgaaagcagg
       61 cttactgtct gtaacaacag atgccgataa ggcaacacat caggctttgg aatttttaaa
      121 tattacggta aatagacaag ccgcaatttt aagctatatc gatatctttc attatttgac
      181 tttatttatt gtcgtttcag tgcctttggt tttatttgcc aaaacataca aaataaataa
      241 agacatgatg gaaggacatt gatgcaatat tattctatcc atggcgcatt atacacgtcg
      301 tagaaatagc ctattttaat ttgagaataa tcaatccaat agctaccagc tttacttttt
      361 ctgatactat taatcgtatt aaaaccattg attgccacat cgacgcgctg tgatgcatta
      421 gccaaattgc ttacgttggc aggtgtttta atagtatagc ctttattaaa ctctgatgat
      481 ttattggtgc caacaaatat atcgccaaaa aacaccaatt gatgttgtgt taattgagtc
      541 acaacttgat ttaataatgc gaactgtgcc attaaaggtt gatcccacat aggttgtaaa
      601 atataaatct gttcgtgcgc accaaattga atagctgctt gcgtttgcaa atccatatat
      661 tggctgcaat tgcctattgc taattgttgt tctgctaatt cttgtagtct aaatgcttcg
      721 cgaccttcat tcgttagcca cgcttcattt tttatgtttc ctgcacctat ttttttattt
      781 aaaataccaa ggcatccaat gtcagaaagt acatctaatt gaccctgaat aagtacatca
      841 ctcgttgcca gcaatacact tgaaacatct gaaccgttaa atggaatatt gatgttgttt
      901 ttcatcagcg tttgtcttag catcaatgcc atcaccaagc cattgcgcac ttcattagcc
      961 gcaaaaatac ctaattgcgt ccacataaat tcaggatatt ttcgttgtaa ggcatcgtag
     1021 ctcattaaca caaaagcatt ttttgtcaat acaattttag ctggtgaacc attataaaat
     1081 gcatttgctt tttgttctac taatgcaata tgtgctaaga aattactcaa tatttggtcg
     1141 tacgtatttt ctatggaaat tgtagtatct acattgtcag ataaggcgtc tatgtcatta
     1201 gcgatagctc ttatttcatt taaaaatctg cgttcattgc cgctgaattg cacagaatca
     1261 tagctgtatt tttgtaaaga ttgtaaaagt tcattacgtt gtgtattgtc aatacaatct
     1321 atatttttat cttttttaca ctgtgttaat cctgaaattg cagtaaatac aataaataga
     1381 atataaatag gacgcatagc gaaattttgg caaagttact taatatttaa gacataaaaa
     1441 aaacgtagtc ttaatccata agattgatag gtattttttg cttttaataa ataactgcta
     1501 actttgcaca atgatatata cagcagaaat aaacgtaatg ccattaaagg aattattgga
     1561 tccgcaaggc aaagccgtag agcaaagttt acacaattta ggttttcaaa cttctaatgt
     1621 gcgtattgga aagcatatta ctttaaaaat tgaggctgct tcgaaagaag aagcaagtgc
     1681 aaaagtagaa gaagcctgca aaaagctcct tcataatcct gtaatggaat attttgagtt
     1741 tgttgtaagc gaataagaat tatgttttct gcggtttttt ctttgccgca ggaaataaaa
     1801 tgttatttag aattaatcga tagccagcag aatttggatg caggtttaaa tctgtaacag
     1861 gatcaccaac acgatgctga taatcttcag ggtcgtgccc gccataaaat gtccatgtgc
     1921 ctttgccata aacaccatgt atatatttag cttcatttag gcttttattt tcgcccatca
     1981 ccaacacatc tgattttata aattgttttt tataagcagt ggtttgtccc ataaatcctt
     2041 ttattaattt ctcatgattt tgtgtaagca ttgctggaac ttgatcccat tttgcggaga
     2101 aatcaaataa ggtaaagtaa tccatgtctt ttgagaaagg ccgaacgcca ggaacgtcaa
     2161 tcgtagaaaa ctcgtattca taaggattgc tgcttatcgt ataattttga aaggcaaatc
     2221 cttttgagaa atctaatttt tcttgcgcat tcggatccat tgggtcgtgg tcgaaaggtg
     2281 ttgcgcatat atctacacca gcagcagcta atgcaatatc ataggagtcc gtagcagaac
     2341 acatcgcaaa cataaaaccg ccgcctagta caaaatctct gattttttgt gctacagcta
     2401 atttttctat agaaaccttt ttgaaaccta aggcagccgc tctggtttca taatctgcta
     2461 cttgtttttt ataccaatct gcattttgat aggctgcata aaattttcca tattgacctg
     2521 tgaaatcttc gtgatgtaag tgcaaccaat cgtataaatg caaattgcca tctaatattt
     2581 cttcatcgta aataactgta taaggaattt cagcatagct caacactaaa gtgacagcat
     2641 cgtcccaagg attattggtc tttggagaat agacagcaac tttgggtgct ttttctaaac
     2701 gaacagcatc catatttatt tctggatttg caatgtcatc cataatttta ctcattgttg
     2761 catctgcaat ggtttcgtat tttacatttc gcaccaaaca ttctttttca aattctttat
     2821 tataaggaaa agagaaactt ccacctctgt aattgagcag ccaatcgata ggcacatttt
     2881 tacttaatat ccaataagca ataccataag ctttcaaatg attgctttgt gattcgtcca
     2941 ttggaataag gataaacgaa gctttggcat aagtcgatat acctaataaa atacagaaaa
     3001 tgattttttt cattctccca aaattactat aattttaatt ggcttttggt taaaaatttt
     3061 taaggaaaat taaaagccat aatgctgaaa tagcaggtct atcgccgctt gttgtgatgg
     3121 ataattattt ttcaatttta tttttaatgc ttgcaagctt cgcgtgtccg ttgtcaataa
     3181 gctatacatt tcaggaaatg taattgctga aacattgtcg aaaatttcat tattgcccgt
     3241 gtcatataaa tcgtaataaa gtccgtaagg aatccaatct tcgaaaggat gctcgttgaa
     3301 gttgatgtac ttttgttttt gataagtgta aaacaatcct atattggaaa catcttctgc
     3361 ccaagcttcc gacaatccta ttattccgga attgctaaat gttggttctc catagccacc
     3421 atttgtataa atatattcga cataaggcag ccagaaatca gtgccaactt ttgcaaaatg
     3481 actggcatga gctaattcat gatacatagt ttcaataaaa gatgcacgtg ctgttgtcgc
     3541 ataaatacta taataaggcg cataaatatc tggcaattga tcgccgataa tgtaggataa
     3601 tagttcagcg atatcttctg aaacgccaaa taaattcgtg attaagcgtg ttggattggc
     3661 tatttgctgt tgtgtcatgt tatgcagcat cggcgttgca taagaagtgc taattggcgc
     3721 ttcttttcct aaccaaaaat taaacttctt agttggcatt agaaatgaac tattgctggc
     3781 gaatttttta aaagaattca aagcgagtaa agcagcacaa gaatgatgca gtgccgcatt
     3841 attgaatgtt cgtccaatct caatttgtaa atcagtaaag gcgcaagatt ttctaaaacc
     3901 aagcgagtaa gtgtttacgc caaatatccc gaatagattg gccgcatcta gtgtgtgaat
     3961 ttgtaaatag tcgttatcaa atttcaacaa aacctcagct gtgtcagcag taatggcttg
     4021 cgactcaaaa gaaaaatttc cactttcatc agtaaatcca atggccattt ttgtatgttc
     4081 tttgataatg atttgtacgc cttttaatgg tttcaatagg ctgtcaattg ggtcaaaaaa
     4141 cttaatgcta cctgcaaatt gtttgctcgg tgtttcactg gtacgcatag tgttgcctgt
     4201 ttctggcata tataaagcac ttaaaatttc tgcttcatag ttgtcaatgt ttacgctttt
     4261 aggaaccacg ccataatata taccatattg ctttgtgtga gccgtctcgt attgcaagtt
     4321 tttgtctggt atggcatcaa aaggatggtc taataatata atgccttgct tttctagagc
     4381 atgatattgt tgtatgttgt aaatatgaaa ttttacataa tgatgagtgg ctattttctt
     4441 atctagggaa atttcgctac tcaactcgtt ggggtttttg aggcgctgat taatttctac
     4501 agtacaagtt tcagcaattg gtttttcttt tttgcagcta aataaaattg tacaacaaaa
     4561 taataaaatg agtagtcgca tcatagttgg tttttataaa gataccaaat cattttaata
     4621 aaaaagcctc gcaaatttgc gaggcttggt attgtagtat gtgtatttag aaatttttga
     4681 taatcgcatc accaaattct gaacatttaa gtaaggtggc accttccatt aagcgttcaa
     4741 aatcatacgt taccgttttt ttagtaatgg cggtttccat gcctttaata attaaatcag
     4801 cggcttcttg ccagcccata aattctaaca tcatcacacc gctcaaaatt acagagctag
     4861 gatttacttt atcttggttc gcatattttg gtgctgtacc atgcgtagct tcgaagatgg
     4921 ccatacctgt taaatagttg atatttgcgc caggtgcgat accgatacca ccaactaaag
     4981 cagctaatgc atctgaaata taatcgccat ttaggtttaa tgtggcaact acatcatatt
     5041 cgctcggtct taaaataatc tgttgtaaga agttgtctgc aatcgcatct ttgatgatta
     5101 attttccagc tgcttccgca tttttttgtg cttcatttgc ttcttctggc ccttcttctg
     5161 ccagtatttt gtcatattga gcccaagtaa acactttgtc gccatattcg cgctctgcta
     5221 atttgtaacc ccattctttg aatttacctt ccgtaaattt catgatattg cctttgtgaa
     5281 ccaacgttac agacttgcgt ttgtgtgcta atgcgaagtt gatagcagaa cgtattaaac
     5341 gctcagaacc gtctaatgat actggcttga tgcctagaga cgaagtttca ggaaaacgaa
     5401 ttttgtcgcc aacattcatt tctgtttgca agaaatgtaa taattttttg caatcgtctg
     5461 taccttgtag ccattcaatt ccagcataga tatcttctgt gttttcgcgg aaaataacca
     5521 tgtccacttg ctctggtact tttgagttag atggtacgcc atcgaaatag cgaacaggtc
     5581 taacgcaagc atataagtct aataattgac gaagtgccac atttaaagag cgaatgccac
     5641 cgccaactgg tgttgttaat ggtcctttta ttgcaattaa atgttcttcg attacagtaa
     5701 gtgtttcatc aggcaaccaa gagctagtct gtttgtatgc tttttcgcct gctaatactt
     5761 ctttccagtg aatttttctc gttccattgt atgctttctc tactgctgca tctaataccc
     5821 taacggatgc acgccagatg tctggccctg tgccatcacc ttcaataaat ggaataatgg
     5881 gttcgttcgg cacttgtaag tggccgtctt tcatcgtgat tttttctgct gacatatcta
     5941 tataattaat aagttaatga atattaaggc taaagattgt tgaacagagg atgttttttc
     6001 acttaatctt tgctgcctat actcatgaaa tttgacgcta caaatatacg cattcttatt
     6061 tctttaacgt aaagtttaca aagaatgttt tatgatttat atcctatttt ttttcggaat
     6121 cgtacattta aagtatgaaa aaacttgtct tcaatttatt ttttttattg tatttgccat
     6181 taagcttgtt cgctcaagaa attaaaataa tgtcgtggaa tatttttatg gtgcctccgt
     6241 ttatttttaa atcatgtcaa acagagcgag cgcatttaca agctgctttt atcaaaaaaa
     6301 atgatcctga tattgtagta cttaatgaag catttatgaa aagtacacga actatactac
     6361 ttgaagaatt acaacatatt ttcccatatc aaacgaagat tacaaagaaa ggttttttta
     6421 aaaccaatag tggtgtttgg attttgagca aatatccaat ttcgaaccag caatttatga
     6481 aatataaaaa taaaaaaggc tctgatattt tttcaaaaaa aggtgctgtt tatatggaag
     6541 taactttgcc acataataaa cttcaaatca ttgctacaca tacacaaagc ctagcaaaat
     6601 atagaactgc acgcgaaaaa caatttacac aattaaaaac aaaattagcc gacgttcatt
     6661 tcacagagag tattcctcaa tttataatag gcgatttgaa ttgtgatttt tatgacactt
     6721 cagcttatca atcaatgttg cagaagttag atgttttgcc atttcatttt actggagaaa
     6781 aatatagttg gaacggcaaa gaaaatgagc tggcttatag tttttttgat actattcaag
     6841 agtctttaga ttatatactt ttaagaaacg cacacaaaga aaaagcatca gttatatcga
     6901 cgacaattat gaatccaaaa caagattcat gcttttgtaa aaacaaatat tttaatatct
     6961 ctgatcacca tcctattatc tcaataattc agctaaaata gacgtttcag tttgatttag
     7021 gtgtagttaa tagtatgaca catgcctttt ctccttttct ttttttatct ttaccatcat
     7081 gcagaatcca aacttagacg ctagtggcga ttatctttcc aaaacagaaa aagaattgga
     7141 aaaggtgttg cgtccgcaag cttttgagca gtttgcaggt cagcctcaaa tcatcgaaaa
     7201 tttatctatt tttatacaag ctgcaaaaca aagagatgag gcactcgacc atgttttgtt
     7261 gcacggtccg cctggattag gaaaaaccac tttgtcgtca attattgcga atgaattagg
     7321 tgtggaattg aaaaccactt caggtcctgt gctagaaaag ccaggtgatt tagcaggctt
     7381 gttgacgaat ttacaagcta atgatgtttt atttattgat gaaattcatc gtttaaatac
     7441 tactgttgaa gaatacttgt attctgctat ggaagattat aaaatcgaca tcatgataga
     7501 cagtggcccg agtgcgcgta gtattcaaat tactttaaat ccttttactt taataggcgc
     7561 aacaacgcgt gctggtttgc tcacatcgcc tttgcgcgct cgctttggta tcaactttag
     7621 attggaatat tacaatgtgg atgtgttgaa gaaaattata aaacgttctg ccagaatatt
     7681 aaatacagaa attgatgaaa aaggtgctac ggaaattgca aaacgtagtc gtggcacacc
     7741 acgtattgct aatgccttgc tacgtcgagt gcgcgatttt gcacagataa agggaaacgg
     7801 tattgttgat ttggaaattt cgaaatatgc actttcacag ctgaacgttg acgaacatgg
     7861 tttagatgaa atggataata aaattttatc tgtcataatc gataaattcg ctggcgggcc
     7921 ggttggtatc aatacaatag caactgcagt tggcgaggaa agtggcacta tcgaagaagt
     7981 gtatgagcca tttttaattc aagaaggtta tttgaaacgc acgccacgag gaagagaagc
     8041 aactgcaaaa gcctacacgc acattggcaa agtgccgcca caacaaacag gaagtttatt
     8101 ttgataattt acgtatgaat gataaacttg taaaaattgc cacgtatttc aatcaatttg
     8161 atgtggactt agcaaaattc aaattggaag aaaatggaat ttattgcttc attaaaaatg
     8221 aacacgtcgc acaattgcaa ttcggcttag ccaattttga gttaatggtg tttcagaaag
     8281 acaaagaagc tgctctaatt atcttagaac agcctgttta attaatttct aatttttatg
     8341 aattattttg cttgtgatgg taagtagata aatccacgaa ctactggcac gataaaatct
     8401 cctttagggg tttgtgacat attgccatgt atattattaa atattgcttg tgtgtaattg
     8461 tttttactca cttttttcct acttttcttg tctaaatatt ctgtaaaggc atttttttct
     8521 accttaggcg ttatgttgta aattggattt ccagttgcaa aatcgatagc ttgcaattga
     8581 cagtttgcgt agctattgct agccatagta gcttgttgtt gattgtaaat ataaacaact
     8641 tcattattca ttgtgttgag caatggcgtt gctgttttgg cgtaaactaa attgcttaat
     8701 gcagtttgat tgaatttttt atcagctact aatttttcaa attgtttgct attgctattg
     8761 ttttcagaga aataatattt ttcaatgcca ccttgcgtat agcttacttc atctaatgga
     8821 tacgcatttc caaaattatt gctaataatt aaagtatgta aggtgctgtc attttctgct
     8881 tttgaagaat gtacgactac atcattgtcg caacgactat atgttccttc aaatagaatt
     8941 tgactactca ataattttcc attagtttta ttgtaaacag ataggcttac atttggaaat
     9001 ccttggtctg tgattgcaac atagttacta aaaacggttg cggctgtgcc attgaaaaca
     9061 ttactacttc caggatattt tacaaagcta tttttgtagc gcttgctcca tacttgcgtg
     9121 atttgtcctg ctctttgctg ataagtgaat ttagcaattt catagttggt gacaatatat
     9181 gcatcaccat tttcatcgac agtaattgct tgttgtattt gatgtttttg atttgccgaa
     9241 tcgcgcggat ttagtaattg caatttgttt tttccagcgt aaagttgttt taataaatat
     9301 tcatgtacga acttgttttt ttccatgtct atacgtggat aataagtgca ttttttagtg
     9361 ttatttttgc ataataaaga agcgcatttt ttgtaatttt ttacattcgc tggtgtgctt
     9421 tttatttgtt tccaatctgt ggtgctaaag taattaaatg tttgctcgcc tgttggtaat
     9481 ttattgttta gataaagtgc atcataactt tctttcaatt ggtcaattag tatatcgcgt
     9541 ttctctttaa tgataggaag cgtatcgcta ttcatatcgt ataaaaatgc atggcttgga
     9601 taacgaatgt ctaaggctat aactttcgct gtgttcgtgc ttatccatac tttagtgaca
     9661 tcaaaaggcg caattgcttg tacatattct tgctgcgttg tttttgtttc atcaagcctg
     9721 ctaccataaa cttttacgaa tgcttctttg ttttccactt tttcattgta tggatattga
     9781 gaaagaaatg tattaagatc aatagacata atacctttta aatttccttc ttttatattt
     9841 attggaaata aaagatgacc agtatttagc attgttaaag gcaactcatt ggaaagattt
     9901 aaataagcgc tttctggatt ttttaaataa tgtctttttt tatcgatgcc attttggaat
     9961 ccatttccag gaatggcaaa ttgatttaca tacgaaagct tatttttgtc aactctaaat
    10021 attaaaagtt tattacgcgt attaccagtc gtaattgtta ggtaatacgt ttgtttttta
    10081 tgtttgtagt aaatgccatt attagaaata cctaagccaa attgtttttt tcctttcgaa
    10141 gcatctactc tataataaat tccagctata gaatcagtgc tcggaaaaaa agatttttga
    10201 gacacatcat cactattgtt gtcatttgca gtaattactc cttttgactt ttgaattgcc
    10261 gtgaatagac gttcatttac gacacttgta ttatatgaaa taggcatttc ttttacaacg
    10321 acaggaaagt catttaatac aaggatattt tcttctgtta aactgtcaat ggtgtttgat
    10381 ttgctattgt atttaacttc ataaggcata aaactgtgtt ttatactttg cgcaaaacaa
    10441 ataataggta aagccttaaa taaaaggacg aaaagaacaa ttttcctcac ggcaggttgg
    10501 gttaataatg aataatagaa taaaaattac ttcttattct tgtttacgtt ttgaataaac
    10561 gcaaatataa tatatgtaag gataatagta cttaaaccaa gccattttaa cagtaaaatt
    10621 tgtgggatta cagaaagaat taacattaac ctaaacttgt tctgtttcca gctgaatgga
    10681 ttgcctttta aactgaacat tggaaactct gctatcatca aaaatgctaa tacaaaagga
    10741 attataattg cagcaatttt tgcatatatc gtaaaatccc aattgctttg caagctccaa
    10801 tcgctaaaaa actgcatgta aaatcctaga ataaatagcg caccagatgg cgtatttaag
    10861 ccgataaagt tttctgtttg tcgagtgtct aaattgaatt tcgccaagcg taaacatgcg
    10921 aaaagtgtgt aaacaaatcc aactaatgca acaggttgtt ctaataaatt aattggagaa
    10981 gatgcagcta ttgttgtaat aaaatttgtt ttagcttgaa tgatattaaa caacataata
    11041 ccaggcaaaa cgccaaacgt taccatgtca gccaatgaat ctaattggcc gcctaactca
    11101 gattttacat tcaatgcgcg tgccacaaat ccatctaaaa aatctgctaa taaagaaata
    11161 ctgagaaata taggaacgag tgtgtattct tgctgcaaac atgcaacaat gccacaacaa
    11221 ccagaaaata aattaagtaa tgtaagtgaa ttgggaatgt gttttaccat actattgttt
    11281 tgccttcaaa gaagtagatt aagcgctaca aagctatgca tttttatcat ctatctgata
    11341 caatttatct aaataatcaa gtactaaatc attcaaggtg ccgctgccag aaagttcact
    11401 catcatacca tacatagctg ctttgcgttt tccaccatct tcagtgccag aaaatttttg
    11461 tgttaaggtt gaaaattgct gttcgtttag tagttttttc aatcctttta cagcgcttaa
    11521 ttgaatgttg ttttttagat gtaataatga aaattgcttc gcttttttta cagcagtttt
    11581 taaatcattt ataaataatt cacctttgcc aacatggata taatttaata aaacatgtaa
    11641 gcttgctggc aattgttgtc tgttgatagt ccaacctagt aaagctagtt cgtcagctac
    11701 ttcatataca tcaatcacat cagaagaaaa agcaaataaa gtagctttgg gattgcctaa
    11761 tattttcaat tctggaagtt cttctttgat cttatttttg acaaacacag tttcttcaaa
    11821 agtttctcgt gccattcgtt tgtaaccttc ttcgccaata tgatgtagtg ctgtccacgc
    11881 tgctgcgatt ggtccaccac cacgagtgcc cataaacgaa ggcgagccgt agatgccgcc
    11941 actccaattt gtttttacat aaaattgtcc tttgcgtaag tttgcatttt tatacaaaac
    12001 aacagatgct ccttttgctg catatccata tttatgtaca tcagccgaca tcgatgttac
    12061 gccatcaatt tcaaaatcga actttggcaa atccacttct tccattttta aaaacggcaa
    12121 tataaatcca ccaacacaag catctacatg caataggcat ttatgttgtt tggcaatttt
    12181 ggcaatagct tctatgtcat caatggcacc ttgtggatag gaaggcgcag aagccacaat
    12241 aaaactgacg tcagtgctta agttcttttt caaatcttct atgtctaact gaaagtctgc
    12301 atttaatttg atgagcttat aatttagttc catcgcatct gcagccttgc aaaatgcagg
    12361 atgtgctgtt tctgccagta acattaatgg attttttgta gtcttatttt tctttgacca
    12421 ttcttttgca gctcgtactg ccatataaat actttctgtg ccaccagatg tcatgcagcc
    12481 acttacttgt tcgtcgccat gcaataatga tgctgttatg cgcaccactt cattttccat
    12541 tttttttaaa ctaggaaaaa cagcaggatt taacgcattt gtagcggaga aagtagcgta
    12601 ggcttctttt atgattttat aataatcgtc accaggataa taaacataac caaaaaattt
    12661 accttctttc caattcatgt catcatgcgt gtaggcttcc cattgttgat gaatctcgtc
    12721 tctgtctaat cctgttgttg gtagtaatgt gctcatgtta gaataaaaat acataaataa
    12781 accaaatact ttatcagcct ttgttaaata ataaatttca tttataaaaa atttgttgct
    12841 cattttttat ggaaaatcaa tgctatctgt ctctcaatat aaaaagagaa gtttatgtta
    12901 gtaaaaacgt ttggaagtgc agtatatggc gtagatgctg ctaccataac gatagaagtc
    12961 aatatttgcc aaggacaaca ttattttatg gttggcttgc cagacaatgc agtgaaagaa
    13021 agctggagtc gtgtggaaag tgcattacgt gtcaataaat accacatgcc acgcattcgt
    13081 attattgtca atttagctcc agctgatata cgcaaagaag gttctgccta cgaccttcct
    13141 attgcgattg gcattttagg agcttcagag caaattaata cggataagtt agctgattat
    13201 atcattatgg gcgaactttc attagacggt aaattgcaac ctataaaagg tgcattgccg
    13261 atagccattc aggcgcaaaa agaaggttac aaaggattta ttttgccaaa agaaaatgcg
    13321 cgtgaggcag caattgtcga aggaatacat gtttttggtg tagaacatat tgacgaagtt
    13381 gttgagtttt tagacaataa gaaagaatta gaaccgacag ttgtatttgt cgaagatgat
    13441 tttaataata gcctacaaaa caatcttttc gactttgcag atgttaaagg ccaagaaaac
    13501 attaaacgag cattagaaat tgcagctgct ggcggacata atgtaatttt aattgggcca
    13561 ccaggcgctg gtaaaacaat gcttgccaaa cgcatgccta ctatcttgcc accattgtca
    13621 ttgcaagaag ctttagaaac aacaaaaatt cattcagtag caggaaagct taaatgcaat
    13681 tcagctttat tatatcaacg tccttttaga tcgccacatc atacggtctc taatgctgca
    13741 ttagtaggtg gaggcagtaa tccacaacca ggcgaaattt ctttagcgca taatggcgtt
    13801 ttgtttctag atgaattgcc tgagtttaaa cgagatgttt tagaagtcat gcgccagccg
    13861 atggaagaaa gaaaagtcaa catctcacgc actcgatttt ctattgatta tccagctaat
    13921 tttatgttgg tcgccagtat gaatccatgt ccttgtggat attataatca tccagaaaaa
    13981 gattgcgttt gcgcaccagg cgttgttcat aaatatttga ataaaatttc aggcccattg
    14041 ttagacagaa ttgatttaca tgttgaagta acaccagtga aattagaaga tttatcttca
    14101 aaaactgttt atgaaaaaag taacgctatt cgtaagcgtg taatcgctgc cagacagata
    14161 caagaagcgc gcttttcggc agacgaaata cattgtaatg cgcaaatgag cacaaatctt
    14221 acacaaacgt ggtgtggttt agataatact tctcaaaatt tactaaatgc tgcaatgaag
    14281 cgattaggtt tgtcagcacg cgcttatgaa cggattatac gagttgcgcg tacaatcgct
    14341 gatttagata gtagcgaaaa gatatgcaac gaacacattg cagaagcaat acaatttaga
    14401 agtttagaca gagaaagttg gtcggcatga tagctgaatg aaattagatg ataaatgtgt
    14461 aaagttaaaa gaagtaataa ttagttgggc gacataatgt atttcatatt caaacacaag
    14521 tgttgtaatt aacatacgtt agtacttatt cttatagaat tgctttcaga cttttatttt
    14581 tgttgaaatg gaatgaatat gctcagcgat tttttacaac cgatacaaca acaattatta
    14641 gataattatt atcggcctaa taaatatgcg aatgcataca tacatcttta tcaagaaaaa
    14701 caaaccttag aaaattattc gattgcatta ttaggtgtgc aagattatag aggctacatt
    14761 gcaaataaag gtactgaaca tgctgcagat gaagtacgta aacaattgta ccaattttca
    14821 gcgttcacaa atggtgtgca actgctcgat ttaggcaata ttcaagctgg agaaacatac
    14881 gtagatactt tagttgcttt agttgaaacc ttacacgaat tgtttaaacg caatataata
    14941 actattattc ttggtggcga tgctgctctg gcatctgctc aatttaaggc atacgaaatt
    15001 tttgaaaatc cgattaccgt atctcatgtt gcttcaaatg tattgtggga caatagcgat
    15061 atggatattc ataaaacata tctcaaagat tttttcttaa gtaacttttt aaaaaaatac
    15121 aatttggtgg cataccaatc ttattttatg cacgaaaaac aactatcttt tttaagcagt
    15181 aaagatcatg atgttttaag agtaggaaaa ataagagagc atgtttttga agctgaacca
    15241 catttgcgag aagcagcaat ggtcgccttt aatatcaacg caattcgcta ttctgacgca
    15301 ccagcacaac ctgagccatc tccaaatgga atgtttggcg atgaagcttg tgccgtagca
    15361 cgatatgccg gcatgagcga tcagttaatg agcataggtt tttacgatta taatccaaca
    15421 ttagataaaa gcaatattac agcaaagcaa gtagcgcaaa tgctttggta ttttatggaa
    15481 ggcattagcc tgcgcagaca cgattatcca atcgtaaatg aaaacgattt tagtcaatat
    15541 attgtacaaa tcgaagatgc tgaaaatgat tttattttct tgaaaagcag aaaaagtgat
    15601 agatggtgga tgaaaataat gtatgataaa aaggatttac aaaaacacaa actaattcct
    15661 tgcacttata aagattatct taccgcatta aataatgaga ttccggaacg ttggatgaga
    15721 gcgtggatgc gcctgagtta aaattctact tccaaagctt gttaagattc gttaagtaac
    15781 aataataaaa aaactatttt tcaatttaac ggtttttaag tggaatcttt gtattctcat
    15841 ttttatttat gcgtcgattt gttgccttac tactattttc attttcattt tctattttat
    15901 tggctaatga ttttacagtc agaggaaaaa ttattgacca aaaggatcat tcaccgctaa
    15961 taggtgtaaa tattatactt tccaatacct tagacacact aaaaacacag ggcacttcta
    16021 cagatgttga aggtgatttt tcttttgcac ttgtggatag tggtgagtat ttgctgacag
    16081 tcagctatat cagttatcag actatccaaa aaaatatcac aataaaaaac aatgtcgatt
    16141 tgcccgaaat tgcaatgaaa gaagatagta aattattgaa agaagtagtt atcgaagata
    16201 agcaagtacg cgtacaacaa ctcggcgata cttcacaata taatgccaat gcttttaaag
    16261 taaatagcga tgcgagtaca gaagatttat tacaaaaaat gccaggagtc acatctgaga
    16321 atggtactgt gaaagtgaat ggggaagagg taaaaaaaat attgattgat ggcagacaat
    16381 tttttggcga tgatacaaga acagcagtac aaaatattcc ggctgatatt gtcgataaaa
    16441 tccaagtatt tgataaactt agcgatcaat cacagtttac tggatttgat gacggcaact
    16501 cacaaaaaac aataaatatt atcacaaaaa atggcatcac caattccaag tttggtaaat
    16561 tttatgctgg atatggagga ccaaataatc gctattctgc tggcttgaat tatagcgcat
    16621 ttaaaggcga tagacgtttc accatattag cgatgagcaa caacatcaat cagcaaaatt
    16681 ttaatatcca agatttaatt ggctctatgg gaagcggaaa tgatagacaa ggtgctggcg
    16741 gaagaggtgg tcctggaaga aatagttcta taggtaattt tctagtgggt cagcaaaatg
    16801 gaattgcaac gacttctgca ttgggtttaa actattcgaa taaaataggc aagcaaaaaa
    16861 aagtaacatt atcaggttct tattttttta acggatcatt aaatgagaat aatagcgcta
    16921 gtacgcgaaa ttatatttca agtaatgata gtggtttagt gtacaaagaa gataaagata
    16981 tcgacagtaa aaattttaat aatagattga attttaaaat tgaatatgca atcgattctt
    17041 ccaatgcgct cacctttaca ccaagcataa gcacacaaaa ttatatttcg acatcaaact
    17101 tattggctaa taatttttcg aatacaaacc attcatcaag taacacagaa aataaacaac
    17161 actcaaaaag ttttggtatc aatttttcaa atgatatact ctatcagcat aagtttaaca
    17221 aaaaaggaag aacgatatcg acaaatctaa atactgttgt taattctaaa gctaatactg
    17281 ctagcttata tgcgctaaat acagatagaa tagatacact catcgcgacg gatactcttg
    17341 accaaaaatc aacattgtat gctttaagct atacgattgg tggtaatatt gtgtacactg
    17401 agccaataaa aaagaatggt caaattgcaa taaatttcac gccatctttt acaagatcta
    17461 catctgataa aaatacaaac aatttcgacc ttgcaagcga tagttattca attacagatt
    17521 cgatacttag taatcatttt atcaataaat atattaccaa taaaattgga ataacttatc
    17581 gctataatga tgctaaaaag ttgagttggt caatcggaat ttctggacaa agtgctttgc
    17641 taaaaaatga agtaatcgat tttacagcaa cgccagtttc acgacacttt ttatctgtat
    17701 tacctaatgc tgatttgaat tacaaatttt caaaaaaaga aaatctacgt ttcttctata
    17761 gaacgagtaa taatccgcca agtatttctc aattacaaaa tgtaatagat aacagcaatt
    17821 cgcttatttt aacatcaggt aatgagatgc tgaaacaaaa tttctcccaa acgattggta
    17881 tccgatacag tagagcaaac acagcaaaag ctaccaactt ctttttattt accaatgcta
    17941 ctaacaccat caattacata gcgaatgcta ctacaatttt cagtcgcgat acaataatac
    18001 aaggtgtaaa aatgaatgca ggtacacaat ttattcaacc tgtaaacctc aatggatatt
    18061 ggaacgtacg cacatttttg acttatggtt ttcctattac aaaaatgaaa agcaatatga
    18121 atgtcaatgc aggttttgtc tatttgcgta cgcccacatt gattaatatg tcaaaaaata
    18181 ttgcgaatgc ttatacacta aatgctggca ttacgctcag tagtaatatt agcaaaaaaa
    18241 tagacttcac gctgtcttat catgctaatt ataatattgt gagaaatacc ttgcaacgta
    18301 taaataatgc taactattat aatcatacag ctacagcgcg ttttaattat cagttctgga
    18361 agggttttgt ttttaatacg tcagttagca atacactcaa tgcaggtggt tcatcaaatt
    18421 ataacacgag ctattggttg ttaaatgctt ctttggcata taaatttttg aaagacgaaa
    18481 gtttagaagt aaaattcaca gctaatgata ttttaaatca aaataaaaat atcactagaa
    18541 atgtgacaga ggtttatgtt gaagatatcc aatctactgc attgcgcaga tattttatgg
    18601 gtacgattac ctattcctta cgcaagttgg gcacaggaaa tgcagacgct gataaaccaa
    18661 aagactttat gcttttgcca ccgccatcag gcatgccacc gccaccaggg cattaattat
    18721 tctatcactt tctccatttt catgcttcga gagcctttca ataaaatgct agcatcggaa
    18781 aatgattgtt ggcgaaacca ttgctgcgct tctaaagcat ttgtgaactt taaaacagca
    18841 tcaatagtat gtgtagcgtg aaatgctttt ccaactaaaa ttattttatc aaaatgcaga
    18901 gcagtagcca aattagctat tttttgatgc tcttcagcag tatattctcc tagctcaaac
    18961 atatcgccga gtataacaat cttatttttt gaagttgctt ttgtaaaact ttctaatgca
    19021 tgttgcatgc ttgttggatt agcattgtag gcatctaaaa tcaattcgta actgtctttt
    19081 tttattactt gtgagcgcgc atttgttgaa tgatattgtt caatagcatg ctttattttt
    19141 tgattttcaa tgtcaaaata ttttccgacg gcaatagcac acataatatt gctataatta
    19201 taactgccaa ataaattact gttgataata gttccttcaa agtttaatct taaaaatgga
    19261 ttgtctattt cgataatact atggtattta ttgtcttcac tgttgccgta agtaatgaca
    19321 gatgaaatat ctcttgttgt agccatcttc aataagatag catcatctcc atttacaaat
    19381 atagttccct ttgtgctttt gatgtaatca tagagctctc ctttgcctct ttgaatgcct
    19441 tcaattcccc caaagccttc catgtgcgct ttgccacaat tggtaataat accatgcgtc
    19501 ggttctacat aagcacagta gtttgcgatt tcttgctgat ggttggcacc catttctata
    19561 actgcaattt ctgtatcagc tttcatttct aataaggtca atggaattcc gatatgatta
    19621 ttgaggttgc cttttgtata atgtgtacga aacgaagtct ctaatacagc atgtactaat
    19681 tcttttgttg tagttttgcc attagaacct gtaattgcaa ttactgcttt tggcttcact
    19741 tgatgtaaat gataattagc taattgttgc aaagtagcca aaacatcatc aactaaaata
    19801 caatattcat taatagcata ttgttcctca tctataacag catagcttgc accaagttca
    19861 atactctttt ctgcaaagct attgccattg aattgctcac cttttaaggc aaaataaatt
    19921 tgattttttt caatcttcct agtgtcagtt gttatgccat ttgcttgtaa aaaaagcttg
    19981 taaagttctt gtatttccat acgctaaaaa taaaaaagtc ctgaagtaaa ttcaggactt
    20041 tttttataca cttgttatct attatttata ctttctagct ttttgatttt tagagatagt
    20101 ttttttcatt tctccaaaac cttttccacc aaacatatta ccatcaggtg aacctacgcg
    20161 taccattgcg caacggaaac cgatatcgtc tgatgattga tcttcgttta agaatcttct
    20221 tgtaccaggt gacatgtagt aacctctgtc tctccaagaa ccacctttat aaactctagc
    20281 tttgttattt actaaagtag aaacgccata ttggtattca acttgagaag aagaatcgcc
    20341 atcattatag tctaatgcga atgattttct gtagtttctt ctattgctta agtcttcatc
    20401 ttcttcacga acatatttaa ttctgcccat gctgtctaag ccttcatcat aagatggttt
    20461 catgaagata ttaccacgat atgtattcaa atcatcacca tcagcttctg tcattggacg
    20521 gtaaacatct tgtgtccact cgtttacgtt acctgacata ttgaataatc cgaaatcatt
    20581 agggaagtta gtgtgaacgt ctgcagtaat ttctgcattg tcattaagcg caccagcaac
    20641 acccatatag tcaccacgac ctctcataaa gttagccatg aagtcacctt gatttttatt
    20701 gtgtctttga tagcggaatg tagcgccgtc ccaagcataa attcttctgt ctgtaaccac
    20761 ctcttcacca ggtataggca tattacctct taaaccaagc gcagcatatt cccattctgc
    20821 ttctgttgga agtctatagt ctggtaataa gataccatct ttgccattaa catttctttt
    20881 gccagatccg ttaggattta agtcttcttt ttgtttttta gtagcacctt gatataaacc
    20941 tactgcataa gcctctgtgt tgaagttgtc ttcatttttt tggttagcat ttaagtctaa
    21001 atagccttct tttaccaaaa tcatttcatt cacacggtca gtacgccatt tgcagtattc
    21061 tgttgcttgc aaccaattaa caccaacaac aggataagta ttataagcag gatgagtgaa
    21121 ataatatttt acgtatggct cattatatgc taaatttctt ctccagcaca tagagtccgg
    21181 ttttgcactt ttaacaaatt cagggtggtc tgtgccaaat actctttcta accaatagat
    21241 gtattcgtta tagtcaagat ttgttacctc tgtctcatcc atatagaaag aagaaacggt
    21301 tactcttctt tgtacattgt gattttcata taataaatca tcttctgttg cacccatagc
    21361 aaatgtacca ccttctacca atactaagtt tggtccagta atttgttgag tgttaggtga
    21421 tttttcaaat ccaccccatt ttttatcgtt gtagttccaa ccagtagtag aagaggcgcc
    21481 accgcctttg tttccacctt tacaactaac agctaataca cttgcaacaa taatcaagtg
    21541 taagaaattt ttcattcttt tgtaggtcat tatttcggct ttttataaaa ttattccaaa
    21601 gatatagcaa atttattaat ctgcaaaata tttcggctaa ctttatcaac gaatttttat
    21661 gctttttatt gtttatttag tgtaaaattg cataaaatgt tttctgttgc gttttatact
    21721 gtaaaatagc taacaaatag ttaatcttgc acttgtcatg aaatctattt actcaatatt
    21781 ctttctgtgt ttcaatttat gtgtatgcat tgttcaatta caagcgcaac aatatattga
    21841 tgaatcttca attattgagt tgcattggca gcaaaa
//
LOCUS       DAOQQV010000032        21471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_731_length_21471_cov_7.290110, whole genome shotgun sequence.
ACCESSION   DAOQQV010000032 DAOQQV010000000
VERSION     DAOQQV010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21471
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_21471_cov_7.290110"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLL59_05100"
     CDS             <1..265
                     /locus_tag="PLL59_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23216.1"
                     /translation="LNSFLENGLVHKIADDSGVAKFAVCQHGSSKHKHDEQHIHFKCK
                     KCKRIECLHHASIPTLVLPTNYKIETVNLLIEGICGICNTKAT"
     gene            293..913
                     /locus_tag="PLL59_05105"
     CDS             293..913
                     /locus_tag="PLL59_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMY23217.1"
                     /translation="METQKQRIELKKYEDSVHKRFRNYKWVLCGIFAGVSLLFIALTL
                     SYYISHDKQNQVSIRFTPLTYINTFILLFSSILIELAHKQFFKNNYRQYKRFISGSIA
                     LGILFITSQIISCVMMYVDGQTFSYNSASYFYIIAGIHNFFISLGLITWCIHLVKHWN
                     ILSNYATSFVYFTDSIAKSKLNLFTFYWHFLGIIWLYLLLFFLAIK"
     gene            926..1510
                     /locus_tag="PLL59_05110"
     CDS             926..1510
                     /locus_tag="PLL59_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23218.1"
                     /translation="MFGLFKQKKSIQVTDLIWKTERIKYDALIKQLQDNNKHILIYFF
                     EDTKKSIVDELQTASINFSENTSSNAHILVLKSTQILQMSSAENVNIIFVEHYPLLST
                     EQKLKQHILEVLNIQNVTFYTSFEDELFQLFGAEKILAIMEKMGFNENEPMSHNLISS
                     SIEKAQQKIEEKVSIDTNTRQRKDWFVMYASNIS"
     gene            complement(1517..2122)
                     /locus_tag="PLL59_05115"
     CDS             complement(1517..2122)
                     /locus_tag="PLL59_05115"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMY23219.1"
                     /translation="MITYILIGITVVVSVLAFNNQKLLSDFMFNPYVIHQRNQYQRFI
                     TAGFIHGDFFHLFFNMYALFLFGTIVEQAFEILFPGYGNLLYIALYFSGLIMSSTFSY
                     YKHKNNPRYNALGASGAVAAVMFACIIIYPSQKLMIMPIPFFIPSYIVGPLYLFYSYY
                     MSKKGMDNIGHDAHFFGAIWGVIFVVLLWKEAIPNFIAQVF"
     gene            complement(2134..2943)
                     /locus_tag="PLL59_05120"
     CDS             complement(2134..2943)
                     /locus_tag="PLL59_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMY23220.1"
                     /translation="MKNNFIEKYGTWALITGASSGLGVEFAHQLAAKGLNLILVARRK
                     HLLDELAQQLVEKYKIQCQVVEADLTLDNFQHALMNAIADIDVGLLVNNAGMNCEGQF
                     YRGSLDRNLQMLELNTRAPYILAYEFGKKFIEREKGGIVFTSSISAFNAHPFLSHYAA
                     TKAYILSLAESMNYEFKDKNVDVIALCPGMTKSEMTKGMKDGPLLMEAAPVVKTAIDN
                     LGKTAYVIPGFINKTQVFFNSRILNRSGATHFTGALLKKVLPAANKKKPSK"
     gene            complement(2946..3374)
                     /locus_tag="PLL59_05125"
     CDS             complement(2946..3374)
                     /locus_tag="PLL59_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMY23221.1"
                     /translation="MSEIITISVQCSTNASVIWNALTNVEHHKKWYFDIQDFALEKET
                     TFTFYEPGDNKKFKHICTVVDVVPNKLFSHTWTYPELSKGSSLVTWQLKEENGITEVS
                     ITHKGIESFADGGPDFAIENFVAGWNEILHKDLKNYVENK"
     gene            3625..9018
                     /locus_tag="PLL59_05130"
     CDS             3625..9018
                     /locus_tag="PLL59_05130"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HMY23222.1"
                     /translation="MKNIFLIIVSSICLFSACNKYTPENAVRCVMTNFEDEIENNQNL
                     IFTFNKELVKNDSILEKWLDDDLIKISPNVPGRCKWTSTRELIFSPYGEFPAATDFKI
                     TISDKITRNATKTLKLYGENTFEVHTSYLTLIGSHTFWKANNNDETKIELQTDIKFNI
                     PVNPKKAAALIEASVNGEAANFSVITKRPSKILSIVFQQPKQVNEKAKVKIVLKKIGK
                     NMREEQFTEKSSVVAIQKLELEDAETIHNGVAGTVFIGTSQPVDNKYLKNTIQIEPAV
                     QFTASSDNRGITISSDEFSPDQNYEIQFSKEVVGIFGGKLKNNITTDVSFGDIAPIIK
                     FENTKAEYLGAKGSRNVSIVITKSEKVKVTVYKVFQNNIYALKNRGVDWGYHDEYNEA
                     TGSYDFQDYQYYNTDNTGEKIFEKKYKTSDLKDYGHAKLLNIDFSDNFKEFKGIYVVK
                     VEDDEKIWLQDSKIIALSDIGLIVKQDRKNVHVFANSILNTTPLKNIEIAVISTSNQE
                     LGKTKTDKNGYATFEIPDAYKDFQVGMISASSDDSDFNYVLYQSALVDNTRFDVGGKR
                     IYSSNYDTYIYGERDIYRPGETIHLNCIVRTYDWNAVSDMPVKARILLPSGKELFTYK
                     NVLNAQGAVETSIPLAASTPTGTYTFQLLSGNDELLATKNILVEEFMPDRIKVELKTD
                     KEDYKPLDKIGISFAATNYFGPPAANRNYEVNFSLDKNIFSSKDFKDYNFNISKETQY
                     YSTVRNGATDGNGNGFESFEIPAEYSNVGLLEGRVLTTVFDETGRPVNRLKTFNVFTQ
                     DVFYGVKTEKDFFSTKQVIKIPVVAVDKTGKAFATQMANCKIIKRTWETILQKSDGQL
                     KYVSQKKEEIIDNKNVNIRGANSYYAFTANESGDYEFRIAAPNSDNYVSYFFYAYGWS
                     DTYASSFEVNNEGKIDITLDNTSYKIGDKAKALLTCPFDGKILITIERDKIFEHYYVE
                     SKNKTASLEIKLNDTHVPNVYITATLIKPNVNDGMPLNIAYGFAPIMVENNDNKIPIE
                     ITCAAETRSKTKQTIKIKAKANTEMTIAVVDEGILALKDQQSPDPYAYFYAKRALEVD
                     MYSIYPFLFPELSALGGGFDDISKRVNPLSNKRVKLVSVWSGIIKTNTFGNAEFTFDI
                     PQFSGSLRVMAVAYDGKKFGAAEKMMRVADPIVLSTGMPRFVSPNDELTVPISISNTT
                     KNKTIAKLSVQTHGVLENKTIGTITTEIDGNNEKQIDLQLLAKNNIGEGKLIITVQAF
                     NEVFTDTTFITSRPNATFQKFSGFGSLQAGKNTTLTFNNNVPMLFTAKKLIISNSPLV
                     QFGKNLSYLVNYPYGCAEQTISAAFPQLYYNDLANNVNNIKGKNTSAINNVQAAIDKL
                     QSMQLSNGALSYWQGNGSESWWSTIYATHFLLEAQKAGFIVNEKSLHKMFDYLNARLK
                     TKEVEALYYQDGTQKEVIKAEVPYSLYVLSLADESKIAALNYYKSHPELLTQSGRYLI
                     AAAFALKGDKKKFYAMLPKGFVSEKTNAAFNGNFYSDFRDMALVLNTLIEVDPKNTQI
                     NYMAKQVAQTLLTKKYLNTQENVFGFLAMGKIAKAANKENSTASIAVNGKTIAQFNGK
                     NNLEIPNSLITGNSVQINTKGNGKLFYFWEMEGLPTIGSNKESDNFLEVRRVYKDRNG
                     KAYNTSFFQNDLVVVEISIRSTNGISVPNVAITDLLPAGFEIENARLTEIPQLDWLKN
                     STQPEYKDIRDDRVNLIVTATPKPVKYYYLCRAVSLGNYNVGPIGADAMYNGEYHSYF
                     GAKKISITRKQNI"
     gene            complement(9071..10045)
                     /locus_tag="PLL59_05135"
     CDS             complement(9071..10045)
                     /locus_tag="PLL59_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23223.1"
                     /translation="MFYRNLIFISLICIAKLSFSQTISTQKGLTTIVFNTTDAYVTVY
                     LPDDLRNGDKVFGTFEVYNEGLTDEQLAKSLAEVRKYKFNFSNPDNQTSLMQRFINIP
                     TNQLLNLLPLKITSALTITLTEASGKVHTATIKSIDDPILTVDSCLAPTHFLIGEPYR
                     LIGAFDGNGTNTRVSIFNTIFRVLAESPRQCIVQIQDNFVFSDKTVLKVMEDFKPKCA
                     QDVVPVKVKMVAEKSALKKGESINVHVIVRGLKDIALPTQLVINNLTPDIISIKNGNK
                     QVIELNAAMFANNNEMKFDFTITASATGDFELDTHLNLPEYFPGDIEH"
     gene            10184..10762
                     /locus_tag="PLL59_05140"
     CDS             10184..10762
                     /locus_tag="PLL59_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HMY23224.1"
                     /translation="MTLEQTLQSRSNNQCELCADTNNLSIYEVPPHAQARAEDCIYIC
                     QKCDAQLEKKETLDKAHWKCLLDSMWSEVPAVQVVSWRLLSRLRDESWATDALDLLYF
                     DDEMLNWAKATGDHELSAEVEFHKDCNGTLLQNGDTVVLIKSLDVKGSSVNARLGTVI
                     KNIRLVHDNTEQIEGKIESQTIVILTKYVRKG"
     gene            10786..12036
                     /locus_tag="PLL59_05145"
     CDS             10786..12036
                     /locus_tag="PLL59_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMY23225.1"
                     /translation="MLTSSISILSIIIILFIAFNFILEYVLDSLNAKSWDMPIPKELE
                     GIYDTEKYEKARKYHQARAQLAKISSILGTIGITVFILMKGFAWMHLQVATFSDSSII
                     QALLFFGIFSIASDIIGLPFELYGIFVIEEKFGFNKMTWKTYISDKVKGYIIGGIIGG
                     ILLSLFVWFYTIAGNKFWIYAWILFAAFSIFFAMFYTSIIVPLFNKLNPLEEGTLRHK
                     IEAFATKINFPLTNIFVIDGSKRSTKANAYFSGLGSKKTIVLFDTLIKEQTEDELVSV
                     LAHEVGHYKMKHIQKGMFIGILQMGALLFILQLFINKPALSQALGLQTDVPIFHLGLI
                     AFSLLYSPISMITGIFMNIRSRKNEFEADNYAKENIGTGEHLISALKKLSVNNLSNLQ
                     PNKWYVFFHYSHPPLLARVQNLLK"
     gene            12053..13327
                     /locus_tag="PLL59_05150"
     CDS             12053..13327
                     /locus_tag="PLL59_05150"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMY23226.1"
                     /translation="MKRVNIKSILIILLLVAESYHIHAQKLVSNPFIGHSTKNELRVW
                     CMFKNADSVYLKIDNQEAKTFFWHKEQCYKKYLPIICTFSNLNENTDYDISYSFDNRN
                     YYPLITAKTGHDTISDFQFLAGSCAFIPTGINRIVEPFADIDIFNHMQRDTAEMMLWL
                     GDNLYYIFNYKTYKGQLKNNIKARNKRKLSSFLQSKQQYAIWDDHDYGSNNSDSLFVA
                     KHSSLHVFQQFWPNPIQKTNNYYTFKKQDIQFFMLDDRYHKYGDDVVLGKSQLDWLKN
                     ELLLSNATFKFICIGMQALNPLSTKECLYKSKAEYTSLIEFIKQNKIKGIVFLSGDRH
                     HAELMRIQEENLYPLYDFTTSPLTMYPIKISKRSREFNNPYSVANTHYASYNYGKISI
                     SGKNEDRICTIELKNKKGKTIWTYAIKAEELM"
     gene            13464..13772
                     /gene="rplU"
                     /locus_tag="PLL59_05155"
     CDS             13464..13772
                     /gene="rplU"
                     /locus_tag="PLL59_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HMY23227.1"
                     /translation="MYAIVEIGGHQFKVAQNQEIFVYKLDGNAGDKVNFDKVLLVSGD
                     AGTKVGAPTVSGAAVSGTIVEHTKGDKVIVFKKKRRKGYKVKNGFRQSYTKVKIDAIA
                     "
     gene            13799..14047
                     /gene="rpmA"
                     /locus_tag="PLL59_05160"
     CDS             13799..14047
                     /gene="rpmA"
                     /locus_tag="PLL59_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HMY23228.1"
                     /translation="MAHKKGVGSSKNGRESHSKRLGVKLYGGQEAIAGNIIIRQRGTK
                     FHPGEGVGLGKDHTIFATKDGKVLFTKGYKDRTFVHVS"
     gene            complement(14122..14319)
                     /locus_tag="PLL59_05165"
     CDS             complement(14122..14319)
                     /locus_tag="PLL59_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23229.1"
                     /translation="MAKLLTLRNILTLLAIFFFASCSNSHTAEGELKSISSEDNYTTL
                     IPVGIVADSESGEAIYVVARK"
     gene            14393..14860
                     /locus_tag="PLL59_05170"
     CDS             14393..14860
                     /locus_tag="PLL59_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HMY23230.1"
                     /translation="MKIEIWWIGKTFQDFTQKGYTEFLKRIEKFTNIEIVEIQEIKSQ
                     TQAKQLKSLEAEKILNKLKPDDFLILLDEKGKHFTSVEFASFLQKKDNQATKKLIFLI
                     GGAYGFDDKIYERANDKLSLSSMTFSHQLIRLIFMEQLYRAYTIIHNFPYHNE"
     gene            14909..15505
                     /locus_tag="PLL59_05175"
     CDS             14909..15505
                     /locus_tag="PLL59_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23231.1"
                     /translation="MKNLQYPLSFTFKITTFSNDFIVKDNNGMVVSYIKQKMFRFIED
                     INIYSDETKSQLNYNIKADRWLDFSAVYSMTNHAGLYLGKIARKGWSSLWKARYEIYD
                     ENNAQDLIIHEENAWIKVLDGLLCEIPIIGMVSGYFFNPSYIISRQDGTAVARLKKEP
                     SFWGRKFTLNTLNKFETGEEERLVLGLMMMILLERRRG"
     gene            complement(15506..16468)
                     /locus_tag="PLL59_05180"
     CDS             complement(15506..16468)
                     /locus_tag="PLL59_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HMY23232.1"
                     /translation="MKILIIRFSSIGDIVLTSPVIRCLKTQVANSELHFLTKSSFSEI
                     VSSNPFITKVFAINKEISEVRDVLVKEHYDIIIDLHDNLRSRQASTLLKVKTLRYNKQ
                     RFKRFLLTQFNINLLNNHVVDRYFSATKNLNVLYDGKGLDYFIPEQDEIAASNLPFTH
                     LAGYCILVIGAKHFTKRIPQEKLTELCTKITMPIILIGGIEDAYLGAQLEAIDKYKIY
                     NACGNLRINQSASIIKKAKFVITPDTGMMHIAAAFNKRIISIFGGTEKRLGFTPFQNN
                     ASSVMIENTTLSCRPCHKHGLDKCPKSHFKCMQDIDMQKIIDLL"
     gene            complement(16479..16994)
                     /locus_tag="PLL59_05185"
     CDS             complement(16479..16994)
                     /locus_tag="PLL59_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMY23233.1"
                     /translation="MSNYQQQMQEMVTFHQRIFIADTARVIGKVELEDDVSVWFGAVL
                     RGDLNYIKIGKRTNLQENVVVHVDFENPVIIGEDNIIGHGAIVHGATIGSYNLIGMRS
                     TILNGAQIGNYCVIGAHALVTENMVIPDCSMVLGAPGKVTKVLPLEYVKNGVMIGVEE
                     YLKEKNKFLGI"
     gene            17038..17871
                     /locus_tag="PLL59_05190"
     CDS             17038..17871
                     /locus_tag="PLL59_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HMY23234.1"
                     /translation="MRKIISCCIAIVLSSAIFAQDSLTIIKKEVFHFASTRPAFTEGA
                     LLVPTNKAQIEFGTQYSFFNSTTHAIEHPSFVIKYGISKFFEFRVNGTAATYINDSSR
                     QTGLHPISIGLKLKMLNAKRYAPASSFIGGLSANVIATKNFKTTHLAPYFRILMEQFL
                     PKNVGLMYNYGLVWNGEDAKPTYNLAIATTYTKALQNAGEKHHQFKYFFELYAIYPHG
                     EKFDLRINNGFSYLLNKFLQFDVSVGAGLLKNSPRFITSAGLAIRFPKKDKAPKHKEE
                     D"
     gene            17957..18784
                     /locus_tag="PLL59_05195"
     CDS             17957..18784
                     /locus_tag="PLL59_05195"
                     /inference="COORDINATES: protein motif:HMM:NF039623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HMY23235.1"
                     /translation="MASCQKEQSFLLSQKNIKIIGAGTTFYSDEDYLALVELTDTAKI
                     PTIAAFKQSMNKISNIVTKYKDRAGAFPCLYNVVTNVAYETLNNMQTPYAEMGKAFMN
                     EFGKHFLRNFHNYLTGQTIEPAWLNYFKRVAENKNTFLHLGMSGINAHITYDIPFVLD
                     DINAEANFFSDFTVYTDFIASAYPQVAIELNKQYGIQQADDVFKLFIIGDILDANNGE
                     GFTTHLVIDLLRTQSWQRGLDLQNGIKSQSQMHSICYSEFIQRENILQAADDAKLLY"
     gene            18845..20593
                     /locus_tag="PLL59_05200"
     CDS             18845..20593
                     /locus_tag="PLL59_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HMY23236.1"
                     /translation="MRAYSYTLLLIITIISSSCQQKKTTENDKAFEKLAASYIDSYLS
                     WRPQYGTYLGLHEYDGKITDYSDVSIEKELTKLKEFDKKFEAIDVNSISKKNYYDLQM
                     LKLSVKNEIFNIVDLHYYQKNPMAYAGAIDINIYIKRNFAPIEQQIKSIIAIEKEAPK
                     LYENAKKNLADTLSKPHTELAISIAKGTASFLEKDVLIALKEVKDTALLNELKTVNQK
                     AIAAILDYATFLEKEKLPIAKNNYAIGEDNYRKMLQYSEDISMSPDDILALGMKELAK
                     EEARFVAAAKCIDLNKKAVDVYNAMQKEHPTAASLIPDARKHLEGIRQFLIDNKIITM
                     PSEVRVKVEETLPYLRETSTASMDTPGPFEKKATEAYYYITPVNLAWKPEQQEDWLMQ
                     FNYYGTDIVSIHEAYPGHYTQFLHLNASDASKVQKIFGSYAFIEGWAHYTEQMMIEEG
                     FEQKGDSIKAAKYRLAQSGEALLRLCRLVVSIRTHCKGMTVEEATTFFMDNWHQGEKS
                     SQQEALRGTFDPGYLFYTLGKLQILKLKEDYKKQEGKNYSIQKFNDAMTDNGMPPIRI
                     MREILLKDEKTWNEIL"
     gene            complement(20613..20954)
                     /gene="arsC"
                     /locus_tag="PLL59_05205"
     CDS             complement(20613..20954)
                     /gene="arsC"
                     /locus_tag="PLL59_05205"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HMY23237.1"
                     /translation="MITIYHNNRCGKSRSALCILEESHKPFTTVEYLKDTPSIEELKV
                     ILKKLQAKPHDIIRTKEAIYLEKYKGKNLSDEEWLQAMNENPILIERPIVINGDKAVV
                     ARPPEKVNEIL"
     gene            complement(20973..>21471)
                     /locus_tag="PLL59_05210"
     CDS             complement(20973..>21471)
                     /locus_tag="PLL59_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HMY23238.1"
                     /translation="FKQKIKAGEIGSSAMPHKVNPIDFENAEGNLGIANALFTHLAEK
                     LPISRLQRDLTDSTVLRNIGVPLAHTLIAFDSIVKGLNKLLVNEQKIQQDLEQNWIVV
                     AEAIQTVLRREGFDKPYEALKNLTRTNTHIGEKEIHDFIDALLVADPIKEELKKITPF
                     NYAGI"
BASE COUNT         7781 a         3512 c         3516 g         6662 t
ORIGIN      
        1 tttaaattcc tttttagaaa atggattagt acataagatt gctgacgact caggtgtggc
       61 aaaatttgct gtttgtcaac atggaagcag caaacataaa cacgacgaac agcatattca
      121 ttttaaatgt aaaaaatgta agcgcattga gtgtttacat catgccagca taccaacatt
      181 agtattacca acgaactaca aaatagaaac tgtaaatcta ttaatcgaag gaatttgcgg
      241 aatttgtaat actaaagcaa cgtaagtaat aaatattcgt catactatat tgatggaaac
      301 acaaaaacaa cgaatagaat taaaaaaata cgaagattct gtacataaaa gattcagaaa
      361 ttacaaatgg gtactttgtg gcatttttgc aggtgtttcc ttattattta ttgcgcttac
      421 cttatcttat tatatcagtc acgacaaaca aaaccaagta agcatacgct ttacaccatt
      481 aacttacatc aacacgttta tactcttatt tagcagtatt ttaattgaat tggcgcataa
      541 gcaatttttc aaaaacaatt ataggcaata caaacgattt attagtggtt ctattgcatt
      601 aggtattcta tttattacaa gtcaaattat ttcatgtgtg atgatgtatg ttgatggaca
      661 aactttttcc tataattcag catcttattt ctacatcatt gctggtatcc ataatttttt
      721 tatttcatta ggtttgatta cttggtgcat tcacttagta aagcattgga atatattatc
      781 taactacgcc acttcttttg tttattttac agattcgata gcaaaatcga aattaaattt
      841 atttactttt tattggcatt ttttaggtat aatttggcta tatttattgc tattcttttt
      901 agccataaaa taaaaatgca caataatgtt tggactattt aaacaaaaga aatctattca
      961 agttactgat ttaatttgga aaacagaacg catcaagtac gatgctctca tcaagcaatt
     1021 gcaagataac aacaaacata tactgatata ttttttcgaa gacaccaaga aaagtatcgt
     1081 tgatgaattg caaacagcaa gcattaattt ttcagaaaat acaagctcaa atgctcatat
     1141 tttagtgcta aaatcaacac aaatacttca aatgagtagt gctgaaaacg ttaatatcat
     1201 ttttgttgag cactatccat tacttagcac agaacagaaa ttaaaacaac atattcttga
     1261 agtgttaaac atccaaaacg ttacatttta tacatcgttt gaagatgaac tttttcaatt
     1321 atttggcgct gagaaaattt tagcaataat ggaaaaaatg ggctttaatg aaaacgagcc
     1381 tatgtcgcac aatttaatta gtagttctat tgaaaaagca caacagaaaa tagaagaaaa
     1441 agtaagcatc gacacaaaca caagacagcg aaaagattgg tttgtgatgt atgctagtaa
     1501 tattagctaa tatttcctaa aatacttgtg ctataaaatt aggaatcgct tctttccata
     1561 ataaaacaac gaagatgacg ccccaaattg cgccaaaaaa atgagcatca tgtccaatgt
     1621 tgtccatacc ttttttactc atgtaatagg aataaaacaa atacaatggg ccaacaatat
     1681 atgatggaat aaaaaatgga attggcatta tcatcaattt ttgtgatgga taaatgatga
     1741 tacaggcaaa catgactgct gccactgcgc cagatgcacc taaggcatta tagcgtggat
     1801 tatttttgtg tttatagtac gagaatgttg aactcataat caatccagaa aaatataatg
     1861 cgatatacaa taaattgcca tagcctggaa acaatatttc aaatgcttgt tccacgattg
     1921 tgccaaacaa aaacaaggca tacatattaa aaaataaatg aaagaaatcg ccgtgaataa
     1981 atccagctgt aataaatctc tggtattgat tgcgctgatg tatcacataa ggattaaaca
     2041 taaaatcact caataacttt tgattattaa aagccaatac actaacaact actgttattc
     2101 caattaagat atacgttatc atactgctga aaattattta gatggctttt ttttattggc
     2161 cgctggcaat acttttttta gtaatgctcc tgtaaaatga gtagcgccac ttctatttaa
     2221 aattctgcta ttaaagaaaa cttgtgtttt atttataaaa ccaggaatga cgtatgccgt
     2281 cttgcctaaa ttatctattg ctgtttttac cactggagct gcttccatca atagcggacc
     2341 atctttcatg ccttttgtca tttctgattt tgtcatacca ggacataacg caattacatc
     2401 tacgttttta tctttaaact cgtagttcat actttctgcc aaggataaaa tataagcttt
     2461 agttgctgca taatgtgata aaaatggatg cgcattaaat gcactaatac tagaagtaaa
     2521 tacaatgccg cctttttctc tttctataaa ttttttacca aattcatatg ccaatatata
     2581 tggtgctctt gtattaagtt ctagcatttg caggtttcta tctaagctac cacggtaaaa
     2641 ttgtccttca caattcatgc ctgcattatt aaccaataaa ccaacatcaa tatcagcgat
     2701 agcattcatg agtgcatgtt gaaaattatc aagcgttaaa tctgcttcaa caacttgaca
     2761 ttggattttg tatttttcaa ccaattgctg tgccaattca tctaaaagat gttttcttct
     2821 tgctaccaaa atcaagttta aacctttagc agccaattgg tgcgcaaact ccactcctaa
     2881 tccgctcgaa gcaccagtaa ttaaagccca agtgccatat ttttcaataa aattattctt
     2941 catatttatt tattttctac gtaatttttt aagtctttat gtaatatttc attccaacct
     3001 gcaacaaaat tctcgatggc aaaatctggt ccgccgtcag caaatgattc tattccttta
     3061 tgtgtaatcg acacttctgt gatgccattt tcctctttca attgccatgt tactaaagaa
     3121 cttcctttac ttaattcagg ataagtccaa gtatgactaa ataatttatt aggaacaaca
     3181 tcaacgaccg tacaaatatg tttaaatttc ttattgtcac caggttcgta aaatgtgaat
     3241 gtggtctctt tttctaaggc gaaatcttgt atatcaaaat accatttttt atgatgttca
     3301 acattcgtaa gtgcattcca aataacagaa gcattcgtag aacattgaac agaaatagta
     3361 atgatttcac tcataaaatt gtattttgat gattaaagat actactaaat acatttgaaa
     3421 cactaaattt ttaaacaaaa aacaacacta aaaattttat tatactattg ataatacaat
     3481 catgatataa ataaaataaa attaaaaaat ctacattata ctagaggcat acattatcat
     3541 tatattttta gataaaattt taaaaattat acatcgaata ttttaaaata gttgtagatt
     3601 tctaaaaata aaaccaagaa gattatgaaa aatatatttt tgattatagt tagtagcatc
     3661 tgtttatttt cagcttgcaa taagtacacg ccagaaaatg ccgtccgatg tgtgatgacc
     3721 aattttgaag atgaaataga aaataatcaa aatctcattt ttactttcaa taaagaattg
     3781 gtgaaaaatg attccatttt agaaaaatgg ttagatgatg acttaataaa aatatctcca
     3841 aatgttcctg gcagatgcaa atggacttct acaagagagt taattttttc accatatggt
     3901 gagtttccgg ctgctactga ttttaaaatt actatatctg ataaaattac aagaaatgcc
     3961 acaaaaacat taaagctata tggcgaaaat acatttgaag tacatacatc ttatttaaca
     4021 ttaattggtt cacatacttt ttggaaagcc aacaataacg atgaaaccaa aatagaattg
     4081 caaacggata taaaattcaa tatacctgtt aatcctaaaa aagctgcagc attaatagaa
     4141 gcaagcgtaa atggtgaggc tgctaatttt tcagtaatta caaagcgacc atcaaaaatt
     4201 ttatctattg tttttcaaca accaaagcag gtaaacgaaa aagcgaaagt aaaaattgta
     4261 ctaaagaaaa ttggcaaaaa tatgcgcgaa gagcaattca cagaaaaatc gtctgtcgtt
     4321 gccattcaaa aattggaatt ggaagatgca gaaaccattc acaatggtgt cgcaggcact
     4381 gtatttattg gcacatctca acctgtcgac aataagtatt taaaaaacac catacagatt
     4441 gaaccagctg tacagtttac agcaagtagt gataatagag gcattactat ttcgagcgac
     4501 gaattttctc cagatcaaaa ttatgaaatt caattcagta aagaagtcgt aggtattttt
     4561 ggaggaaaat taaagaataa tatcacgaca gatgtttcat ttggcgacat tgctcctatt
     4621 ataaaatttg aaaacacaaa agctgaatac ctcggcgcta aaggcagtcg caatgtttct
     4681 attgtaataa caaaatctga aaaagtaaaa gtaacagtat ataaggtttt ccaaaataat
     4741 atatatgctt taaaaaacag aggtgtcgat tggggttatc acgatgaata taatgaagca
     4801 actggcagct atgattttca agattatcaa tactacaaca cagataatac gggagaaaaa
     4861 atatttgaaa agaaatataa aaccagcgac cttaaagatt atggtcatgc taaattatta
     4921 aacatagatt ttagtgacaa ctttaaagaa tttaaaggaa tctatgttgt aaaagtagaa
     4981 gatgatgaga aaatttggtt gcaagactct aaaataattg cactttctga tattggatta
     5041 atcgtaaaac aggacagaaa aaatgtacat gtttttgcaa attctatatt aaatacaaca
     5101 ccattaaaaa acatagaaat tgctgtaatt agtacttcta atcaagaatt aggcaaaaca
     5161 aaaaccgata aaaatggcta tgctactttt gaaattccag atgcttacaa agactttcaa
     5221 gtaggcatga taagcgcatc gtctgacgat agtgatttca attatgtttt atatcaatcg
     5281 gcactcgtgg acaacacgcg atttgatgtt ggcggcaaga gaatctactc ttcaaattat
     5341 gatacttaca tttatggcga acgagatatt tacaggcctg gagaaacaat acatttgaac
     5401 tgcatcgtac gcacgtatga ttggaatgca gtaagcgata tgcctgtaaa agctcgcatt
     5461 ttattacctt caggtaaaga attattcact tataaaaatg tactgaatgc acaaggtgca
     5521 gtcgaaacat cgatcccgct tgcagcctca acaccaacag gaacttacac ttttcaactt
     5581 ttaagtggca atgatgaatt gctggcgaca aaaaatatat tagttgaaga gtttatgcct
     5641 gacagaatta aagtagaatt aaaaacagat aaggaagatt acaaaccatt agacaaaata
     5701 ggcatttctt tcgctgcaac aaattatttt ggaccacctg cagcaaacag aaactatgaa
     5761 gttaatttta gtttagataa aaatattttc agcagtaaag attttaaaga ttacaacttc
     5821 aatatcagta aagaaacaca atattatagc accgtcagaa acggtgcaac tgacggaaat
     5881 ggcaatggtt ttgaatcgtt tgaaatacct gcagaatata gcaatgtagg cctattggaa
     5941 ggcagagtgc tcactacagt ttttgatgaa acaggacgac ctgtcaatcg cttaaaaaca
     6001 tttaacgtat ttacacaaga tgttttttat ggtgtaaaaa ctgaaaaaga ttttttcagt
     6061 actaaacaag ttatcaaaat tcctgtcgtt gcagtagata aaacgggaaa agcatttgcc
     6121 acgcaaatgg caaactgtaa aattataaag cgaacttggg aaaccatttt acaaaaatca
     6181 gatggtcagc ttaaatatgt atcacagaaa aaagaagaaa taatagacaa taaaaatgtc
     6241 aacatacgtg gtgctaattc ttattatgca tttacagcaa atgaaagtgg tgattatgaa
     6301 tttagaatag cagcaccaaa cagtgacaat tatgtgtcct atttctttta tgcttacggt
     6361 tggagtgata cgtatgcaag ctcattcgag gtaaataacg aagggaaaat tgacatcaca
     6421 ttggacaata cttcgtataa aattggcgac aaagctaaag cacttttaac ttgtcctttt
     6481 gatgggaaaa tattaatcac cattgagcgc gacaaaatat ttgaacatta ttatgttgaa
     6541 tcaaaaaata aaacagcgtc tttggaaata aaattaaacg acacacatgt tccaaatgta
     6601 tatatcacag caaccttaat aaaaccaaat gtaaatgatg gcatgccgct caacattgct
     6661 tatggttttg cgcctattat ggtagaaaat aatgacaata aaattccaat agaaattacg
     6721 tgcgctgctg aaacacgcag taaaaccaag caaactataa aaataaaagc aaaagcaaat
     6781 acagaaatga ccattgccgt tgttgatgaa ggtatattag cactaaaaga ccagcaatcg
     6841 cctgatccgt atgcatattt ctatgcaaaa cgtgcattag aagttgatat gtattccatt
     6901 tatccttttt tatttccaga gctaagcgct ttaggtggtg gcttcgatga tatttcaaaa
     6961 agagtgaatc cactaagtaa taagcgcgta aaattagtgt ctgtttggag tggcatcata
     7021 aaaacaaaca catttggaaa tgcggaattt acatttgata ttccacaatt ttcaggcagt
     7081 ttacgtgtaa tggctgtcgc ttacgatggg aaaaaatttg gtgctgctga aaagatgatg
     7141 cgcgtcgcag atccaatagt gttgagcact ggcatgcctc gatttgtatc gccgaatgat
     7201 gaattgactg ttccgattag tatttctaat acaacaaaaa ataaaactat cgcgaagcta
     7261 agtgtacaaa cacatggtgt gttagagaat aaaactatag gcacaataac tactgaaata
     7321 gatggcaata atgagaaaca aattgacttg caattgttag caaagaacaa tattggagaa
     7381 ggtaaattaa tcattacagt gcaagcattt aatgaagttt ttacagatac tacatttatc
     7441 acatcaaggc caaatgcgac atttcaaaaa ttttcaggat ttggcagtct gcaagcagga
     7501 aaaaacacaa cactcacatt caacaacaat gtaccgatgt tatttactgc taaaaaatta
     7561 attatcagca attcaccatt agtacaattt ggaaaaaatt taagttactt agtaaattat
     7621 ccttatggct gcgctgaaca aactatttct gcggcatttc cgcaattata ttacaatgat
     7681 ttagccaaca atgtaaacaa cataaaaggt aaaaatacaa gtgctattaa taacgtacaa
     7741 gctgcaattg acaaactgca atcgatgcaa ttatcaaatg gcgcattgag ttattggcaa
     7801 ggcaatggca gcgaaagttg gtggagcact atctatgcaa cacatttttt attggaagct
     7861 caaaaagctg gattcatcgt caacgaaaaa agtctgcata aaatgtttga ctatttaaat
     7921 gctcgtttaa aaacaaaaga agtagaagca ttgtattatc aagatggcac acaaaaagaa
     7981 gtgattaaag ccgaagtgcc atatagcttg tatgtgttat cattagctga tgaatcaaaa
     8041 attgcagcat taaactatta taaatcacat cctgaattat tgacgcagag tggtcgatat
     8101 ttgattgcgg cagcattcgc tttaaaaggc gataaaaaga aattttatgc catgttgcca
     8161 aaaggatttg ttagcgagaa aacaaacgcc gcatttaatg gtaatttcta ttccgatttt
     8221 cgagatatgg cattagtatt aaatacatta atcgaagtag atccaaaaaa tacgcagatt
     8281 aattacatgg caaaacaagt agcacaaact ttgttgacaa aaaaatatct gaacacacaa
     8341 gaaaatgttt ttggattttt agctatggga aaaattgcga aagcagccaa taaagaaaat
     8401 tctacagcat cgattgctgt taatggaaaa accatagcac aatttaatgg aaaaaataat
     8461 cttgaaattc cgaatagttt gataacaggt aacagtgttc aaatcaacac aaaaggaaat
     8521 ggaaaactat tttatttctg ggaaatggaa ggtttaccga ccataggcag caataaagag
     8581 tcagacaact tcttagaggt acgtcgagtg tataaagata gaaatggtaa agcatacaat
     8641 actagctttt tccaaaatga cttagtagtc gtggaaatca gcatcagaag tacaaatggc
     8701 attagtgtgc caaatgttgc tattacagat ttattgcctg caggatttga aatagaaaat
     8761 gcgagattga cagaaattcc gcaattggat tggctgaaaa attcaactca acctgagtac
     8821 aaagatattc gcgatgacag agtaaattta atagtaacag caacgcctaa gcctgtgaaa
     8881 tattattatc tgtgcagagc tgtttcctta ggtaattata atgttggacc aattggcgca
     8941 gacgccatgt acaatggtga gtatcattcc tactttggcg cgaaaaaaat tagtattaca
     9001 agaaaacaaa acatttaaaa tcataagcat gcacaattag ttaaacacaa atagttgcat
     9061 cttagtagtg ttaatgttcg atatcgccgg gaaaatattc aggcaaatta aggtgtgtat
     9121 ctaattcaaa gtcgccagtt gcgcttgctg ttattgtgaa atcaaatttc atttcattgt
     9181 tattggcaaa catagctgca ttcaattcta taacttgttt attgccattt tttatgctaa
     9241 taatatctgg cgttaaatta tttattacta gttgtgttgg caatgcaata tctttcaaac
     9301 ctctaacaat tacatgtaca tttatcgatt cacctttttt caaagcactt ttctcagcga
     9361 ccattttcac ttttacaggc acaacatctt gtgcgcactt aggtttaaaa tcttccatta
     9421 ctttcaaaac agttttatca gaaaaaacaa aattatcctg aatttgcaca atgcattgtc
     9481 gtggcgattc tgctagcaca cgaaaaatcg tattaaaaat actcactctt gtattggtgc
     9541 catttccatc aaatgcacca attaacctat acggttcgcc aattaaaaaa tgcgttggcg
     9601 ccaaacatga atcgactgta agaattggat cgtcgataga tttaatagtg gctgtatgca
     9661 cctttcctga tgcttctgtg agcgtgatgg ttaatgcgga agtaattttt aatggcaata
     9721 aattcaacaa ctgattggtc ggaatattta taaaacgttg catcaagctc gtttggttat
     9781 caggatttga aaagttgaat ttatactttc gcacttctgc taatgatttt gccaattgct
     9841 cgtcagttaa gccttcgtta tacacttcaa acgtgccgaa tactttatcg ccatttcgta
     9901 agtcatcagg caaatatacc gtcacataag catcagtagt gttaaaaaca atagtggtaa
     9961 gtcctttttg tgtagaaatt gtttgcgaga aagacaactt tgctatgcaa atcaatgata
    10021 taaaaatgag atttctgtaa aacatacttt attattaaaa ataaaattac aaatatcgtg
    10081 caagaattca atgacaatga attagaaatt aaaatgttat gtgtaatgct attgttatcg
    10141 atttaaaata cacagtttaa ttcattattt ttgcaaaaaa aacatgacat tagaacaaac
    10201 gcttcaatca cgaagtaata atcaatgcga attatgtgca gacaccaaca atttgagcat
    10261 ttatgaagtg ccaccacatg cacaagcacg cgctgaagat tgcatatata tttgtcaaaa
    10321 gtgcgatgca caattagaaa aaaaagaaac tttagataaa gcacactgga aatgcttatt
    10381 agatagcatg tggagtgaag tgcctgctgt acaagtcgtt tcttggcgtt tgctcagtag
    10441 attacgcgat gaaagttggg caactgatgc tttagattta ttatattttg atgatgaaat
    10501 gctgaattgg gcaaaagcca ccggcgacca cgagctttct gcggaagtag aatttcacaa
    10561 agattgcaat ggcaccttat tacaaaatgg cgatactgtc gtattgataa aatcattaga
    10621 cgtaaaaggc tcttccgtaa atgctcgctt aggcactgtc atcaaaaaca tacgattagt
    10681 acatgacaat acagaacaaa tagaagggaa aatcgaaagt cagacgatcg taattcttac
    10741 aaaatatgtt cgaaaaggtt aattcattga ataaataaaa atattatgct cacttctagc
    10801 atctcaatac tcagcattat catcatctta tttattgctt ttaatttcat cttggaatat
    10861 gtattagatt ccttaaatgc taaaagttgg gacatgccta ttccaaaaga attagaagga
    10921 atttatgaca cagaaaaata tgagaaggca agaaaatatc atcaagcaag agcacaatta
    10981 gctaagatat cgagtatatt gggcactatt ggtattactg tttttatttt aatgaaaggt
    11041 tttgcatgga tgcatttgca agtggctaca tttagtgatt catcaatcat acaagcactt
    11101 ctattttttg gaattttttc aatagcatca gatattattg gattgccatt tgaactatat
    11161 ggcatttttg taattgaaga gaaattcggt tttaataaaa tgacatggaa aacttacatt
    11221 agcgataaag taaaaggcta cataattggt ggtattattg gtggcatact tttgagttta
    11281 tttgtttggt tttataccat tgctggcaat aaattttgga tatatgcttg gattttattt
    11341 gcagcatttt ctattttctt cgctatgttt tacacaagca ttattgtgcc acttttcaac
    11401 aaactcaatc cgttagaaga aggcacatta cgacataaga tagaagcatt tgctacaaaa
    11461 ataaatttcc ctttgacgaa tatatttgta atagatggtt caaagcgcag cactaaagca
    11521 aatgcctatt ttagtggact tggcagcaag aaaacaatcg ttttatttga tacactcatc
    11581 aaggaacaaa cagaagacga actcgtgagt gtgttggcgc acgaagttgg tcactacaaa
    11641 atgaaacaca ttcaaaaagg tatgtttatt ggtattttgc aaatgggcgc acttttgttt
    11701 atactgcaat tattcatcaa caagcctgcc ttatcgcaag cattaggtct gcaaacagat
    11761 gttccgatat ttcatctcgg tttgattgca tttagtttat tatacagtcc aatatccatg
    11821 ataacaggca tcttcatgaa tattcgctct cgtaaaaatg aatttgaggc ggataattat
    11881 gcgaaagaaa atataggcac tggcgagcat cttatttctg cacttaaaaa attatctgtg
    11941 aataatttat ccaatctcca accaaataaa tggtatgtat tttttcatta ttcgcatcca
    12001 ccattattag cacgcgtaca aaatctattg aaataaaatc gaacaactct cattgaaacg
    12061 agtaaacata aagtccatat tgattatact gctactagta gcagaaagct atcatataca
    12121 tgcacaaaaa ttagtttcta atccatttat cggccatagc acaaaaaatg aattacgtgt
    12181 ttggtgtatg tttaaaaatg ctgactctgt ttatttaaag attgacaatc aagaagcaaa
    12241 aacttttttt tggcataaag aacaatgtta taaaaaatat ttgcctatca tttgtacatt
    12301 ttccaatttg aatgagaata cagattatga cattagttat tcatttgaca ataggaatta
    12361 ttatccatta atcactgcaa aaactggaca cgacaccatt agtgattttc aattccttgc
    12421 aggctcttgc gcttttatac caactggaat aaatagaatt gtagagccat ttgccgatat
    12481 tgatattttt aatcacatgc aacgcgacac agcagaaatg atgctgtggc tgggcgataa
    12541 cttatattat atcttcaatt acaaaacata taaaggtcaa cttaaaaaca atataaaagc
    12601 tcgaaataaa agaaaactaa gttcattttt gcaatccaaa cagcaatatg ccatttggga
    12661 cgatcacgat tatggaagca ataatagcga tagtctgttt gttgccaaac actcatcatt
    12721 acatgtattt caacagtttt ggcctaatcc aatacaaaaa acaaacaatt actatacctt
    12781 caaaaaacaa gatattcagt tttttatgct agatgacaga tatcataaat atggcgatga
    12841 tgtggtgctg ggaaaatcgc aattagactg gctaaaaaat gagcttttac tttcaaatgc
    12901 tacttttaaa tttatttgta ttggcatgca agcattaaat ccattgagca caaaagaatg
    12961 tttgtacaag tcaaaagcag aatatacttc cttaattgaa tttataaagc aaaataaaat
    13021 aaaaggcatc gtgtttttat ctggcgacag gcaccatgca gaactgatgc gcatacaaga
    13081 agaaaatctt tatccattat acgattttac gacttctcct ttaacgatgt atcctattaa
    13141 aatatcaaaa agaagtagag aatttaacaa tccatattca gtagcgaata cgcattatgc
    13201 atcgtataat tatgggaaaa tttccatttc aggtaaaaat gaagacagga tttgcaccat
    13261 tgaattgaaa aataaaaaag gcaaaaccat atggacttat gcaatcaaag ccgaggaatt
    13321 aatgtaaaac aatgattatc aaatacataa taaatcgaga aagaacgaat gtggataatg
    13381 tgtggactta ataaaaaatc aacattatca ctgtaaaaaa tcaataaaaa taactatctt
    13441 tgcaactcca aaaaagtaga atcatgtacg caatcgtaga aataggtggt catcaattca
    13501 aagtagcaca aaaccaagag atttttgtgt ataaactaga tggcaatgcc ggtgacaaag
    13561 taaactttga caaagtgtta ttagtatcag gtgatgccgg tacaaaagta ggtgctccaa
    13621 cagttagtgg tgccgctgta tcaggtacta tcgttgaaca taccaaaggt gacaaagtaa
    13681 tcgttttcaa aaagaaaaga agaaaaggtt ataaagttaa aaatggtttc agacaatctt
    13741 atactaaagt aaaaattgac gcaatagcgt aattcattaa ctaaaaaatt ctaataaaat
    13801 ggctcataag aaaggtgtcg gtagctccaa aaacggtaga gaatcacaca gcaagcgatt
    13861 aggtgttaaa ttatatggcg gacaagaggc aattgccgga aatatcatta tccgtcaacg
    13921 tggcactaaa tttcatccag gcgaaggtgt tggcttaggt aaagaccata ctattttcgc
    13981 aactaaagac ggtaaagttt tgtttactaa aggatacaaa gacagaactt ttgttcatgt
    14041 atcataagta atacagcaaa taaaaagaaa gcgccatccg taaggatggc gttttctttt
    14101 ttccgtttta tggtttaggt attattttct agctacaaca taaatggcct cgccactctc
    14161 cgaatctgca acaatgccta ccggaattaa tgttgtataa ttatcttcag aagaaatgga
    14221 ctttaactca ccttcagctg tatgagaatt actgcaagat gcgaaaaaaa atatcgctaa
    14281 gagagttagt atgtttctaa gagtgagtaa ctttgccata actcttagat ttgactacaa
    14341 attaaaactt ttcttgatat aagtcaagaa tacttttgtt aaaaaactgc tcatgaaaat
    14401 agaaatttgg tggataggca aaacttttca ggattttaca caaaaaggtt atactgaatt
    14461 tctaaaaaga atcgaaaaat ttacaaatat agaaatcgtt gaaattcaag aaataaaaag
    14521 tcaaacccaa gctaaacagt taaaaagcct tgaagctgaa aagatattga acaaattaaa
    14581 acctgacgac tttctgatat tattagacga aaaaggaaaa cattttacgt ctgtcgaatt
    14641 tgccagtttc ctgcaaaaaa aagataatca agcaacgaaa aagctcattt ttttaattgg
    14701 tggcgcttat ggttttgatg acaaaattta tgaaagagcc aatgataaat tatcgctttc
    14761 atctatgaca ttctcacatc aactaattcg attgattttt atggaacaac tatatagagc
    14821 gtacaccatt attcataatt ttccatatca caatgaataa tggaactatt tccataaatt
    14881 ttgaattata cacacaataa ctatacaaat gaaaaattta cagtatccac tgagctttac
    14941 ttttaaaatc acaacttttt ctaatgactt tattgtaaaa gacaataatg gaatggtggt
    15001 gagctacatt aaacaaaaaa tgtttcgatt tatcgaagat ataaatatct atagcgacga
    15061 gacgaaatca caattaaatt ataatataaa agcagacaga tggctggatt tttctgctgt
    15121 ctattctatg acaaatcatg ctggattata tctcggaaaa attgcccgaa aaggttggtc
    15181 atcactttgg aaagcacgtt atgagattta tgacgaaaac aatgctcaag atttaattat
    15241 acacgaagaa aatgcatgga taaaagtttt agatggtctg ctatgtgaaa ttcctattat
    15301 cggtatggtg tctggttatt tcttcaatcc atcatatatc atttcacgac aagatggaac
    15361 tgctgttgct cgattaaaaa aagaaccttc attttggggc cgaaaattca cactcaatac
    15421 actcaataaa tttgaaacag gcgaagaaga acgacttgtt ttaggtttga tgatgatgat
    15481 attattagaa cgacgaagag gataattaga gtaaatcaat aattttttgc atgtctatat
    15541 cttgcataca tttaaaatgt gattttggac atttatctaa gccatgttta tgacatggac
    15601 ggcaggaaag tgtagtgttt tcaatcatta cagacgatgc attattctga aaaggtgtaa
    15661 aacctaatcg cttttcagtg ccaccaaaga tggaaataat tcgcttatta aatgctgctg
    15721 caatatgcat catgcctgta tctggcgtaa ttacaaactt tgcctttttg attatcgatg
    15781 cagattgatt gatgcgtaag ttgccacaag cattataaat tttatattta tcaatggctt
    15841 ctaattgtgc gcctaaatag gcatcttcaa taccaccaat taaaataatt ggcatcgtaa
    15901 ttttagtaca caattctgtt aatttctctt gtggaattcg ttttgtaaaa tgcttcgctc
    15961 ctattactaa aatacaataa cctgcaagat gtgtgaatgg caaattactg gctgctattt
    16021 catcttgttc aggaataaaa taatccaagc ctttgccatc atacaaaaca tttaaatttt
    16081 tggtggcaga aaaatatcta tcaacaacat gattgtttaa taaattaatg ttgaattgtg
    16141 tcaatagaaa acgcttaaaa cgttgtttgt tataacgaag tgtttttact ttcaatagtg
    16201 tgcttgcttg ccgagagcgc aaattatcgt gcaggtcaat gatgatgtca taatgttctt
    16261 ttaccaatac atcacgcact tcactaattt ctttattgat agcaaatact ttagtgataa
    16321 atggattact acttactatt tcactaaatg aagatttcgt aagaaaatgc aattcgctat
    16381 ttgcaacttg tgtttttaag caacgaataa caggactcgt caatacaata tcaccaatag
    16441 aagaaaaacg aatgatgagt attttcatgc aatgttcatt aaatacctaa gaatttattt
    16501 ttctctttta aatactcttc aacaccaatc attacaccat ttttcacata ttctaatggt
    16561 aatactttag ttacttttcc aggcgcgcca agcaccatac tacaatcagg aatcaccata
    16621 ttttctgtca ccaaagcgtg agcaccaatg acacaatagt tgccaatttg tgcaccattt
    16681 aaaatagtag aacgcatgcc tattaaattg taagaaccaa ttgttgcacc atgcacaatt
    16741 gcgccatgtc cgatgatatt atcttcgccg atgattactg gattttcaaa atcaacatgc
    16801 actactacat tctcttgtaa gttagtgcgc ttaccaattt ttatataatt caaatcgccg
    16861 cgcaagactg cgccaaacca cacagacaca tcatcttcta actcaacctt accgattaca
    16921 cgtgcagtat ctgcaataaa aatgcgttga tggaatgtca ccatttcttg catttgttgt
    16981 tggtaattac tcatgcaata aatatagaaa ttaaattttg ctttgtattt ttatagcatg
    17041 agaaaaatta tatcttgctg tatagcaatt gtattaagtt ccgcaatatt tgcccaagat
    17101 tcacttacaa ttattaaaaa agaagtattc cattttgcaa gtactcgacc tgcctttaca
    17161 gaaggcgcgc ttttggtgcc gacaaataaa gcacaaatag aatttggtac tcagtattcg
    17221 ttttttaata gcactacaca tgccatcgag catccatctt tcgtaataaa atatggcatt
    17281 agtaagtttt ttgaatttag agtaaatgga acagcagcaa cgtatatcaa tgattcgagc
    17341 cgacaaactg gattgcatcc aatttctatt ggattaaaat taaaaatgct aaacgcaaaa
    17401 cgctatgcgc ctgcttcttc atttattgga ggtttgagtg ccaatgtgat tgctactaaa
    17461 aatttcaaaa caacgcatct agcaccttat ttcaggattt taatggaaca atttttacct
    17521 aaaaacgttg gcttaatgta taattatggc ttggtctgga atggtgaaga tgcaaagcct
    17581 acatacaatt tagctatcgc aactacttat acaaaagctt tgcaaaatgc aggagaaaaa
    17641 caccatcagt ttaaatattt ttttgaattg tatgccatct atccacacgg agaaaagttt
    17701 gatttacgaa tcaataatgg attttcttat ctgctaaata aattcttgca atttgacgta
    17761 tctgttggtg caggcctgtt aaaaaactcg ccacgcttta ttaccagcgc aggtttggct
    17821 attagatttc ctaaaaaaga caaagcacct aaacacaaag aagaagatta acaacacatt
    17881 agattgtaaa attcatacca aataactaat tttaggtatg aaccataaag caattatctt
    17941 tatttgctgt atttttatgg caagttgtca aaaggaacaa tcctttttgc tatctcaaaa
    18001 aaacataaaa ataattggtg ctggcactac tttttatagc gatgaagatt atctcgcatt
    18061 ggtagagctt actgatacag caaagatccc tacgattgct gcatttaaac aatcaatgaa
    18121 taaaataagc aacatcgtta ctaaatataa agacagagct ggtgcatttc catgcttgta
    18181 taatgttgtc accaatgttg cctacgaaac tctaaataat atgcaaacgc catatgctga
    18241 aatgggaaaa gcatttatga atgaatttgg aaaacatttt cttcgcaatt ttcataacta
    18301 tctgactggt caaaccatag aaccagcatg gctgaattat ttcaaaagag tagctgaaaa
    18361 caaaaataca tttttacatc ttggtatgtc tggtattaat gcacatatta cttacgatat
    18421 tccatttgtt ttagatgata tcaatgcgga agctaatttt tttagcgact ttactgttta
    18481 cactgatttt attgcatcag catatccaca agtggcgatt gaattgaata agcaatacgg
    18541 catacagcaa gccgacgatg tatttaaatt attcattatt ggcgatattt tagatgctaa
    18601 taatggcgaa ggttttacca cacatttggt aatagactta cttagaacac aatcgtggca
    18661 gcgtggttta gacttacaaa acggcataaa atcacaaagt caaatgcact cgatatgtta
    18721 ttccgaattt atacaaagag aaaatatttt acaagctgct gatgatgcca aattattata
    18781 ttagctgtaa taatttttag aatataacaa ctaacaagat tattcaaaat aatatcaaac
    18841 caatatgcgc gcttactcat acacattact actcatcatt acaattataa gtagtagttg
    18901 ccaacaaaaa aaaactactg aaaatgataa agcttttgaa aaattagctg cttcctatat
    18961 cgacagctac ttaagctggc gaccgcaata tggcacttat ttaggcttgc atgaatacga
    19021 tggaaagatt acggattaca gtgatgtttc aatagaaaaa gaattaacta aactaaaaga
    19081 attcgacaaa aaatttgaag ctatcgacgt caatagcatc agcaaaaaaa attattacga
    19141 tttgcagatg cttaaattat ctgtaaaaaa tgagattttc aatatcgtag atttgcacta
    19201 ttatcaaaaa aatccaatgg catatgctgg cgcaattgac ataaatattt atatcaaacg
    19261 aaattttgca cctattgaac aacaaataaa atctatcatt gccattgaaa aggaagcacc
    19321 aaaattatac gaaaatgcga agaaaaattt agcagataca ctttcaaaac cacatacaga
    19381 actagcgatt agcattgcta aaggcactgc ttcatttttg gaaaaagatg tattaattgc
    19441 gttgaaagaa gtgaaagaca ctgcactatt gaatgaacta aaaactgtca accaaaaagc
    19501 gattgctgcc atattagatt atgctacatt tttagaaaaa gagaaattgc caattgccaa
    19561 aaacaactat gctataggcg aagacaatta tagaaaaatg ctacaataca gcgaagatat
    19621 ttctatgtcg ccagatgata ttctggcatt aggcatgaaa gaattagcaa aggaagaagc
    19681 acgttttgta gctgctgcaa aatgtatcga tctaaacaag aaagctgttg atgtatataa
    19741 cgctatgcaa aaagaacatc caacggctgc ttcattaatt cctgatgctc gcaaacattt
    19801 agaaggaatt cgtcagtttt tgattgataa taaaatcatt acgatgccat cagaagttcg
    19861 tgtaaaagtg gaagaaacct tgccttactt acgtgaaaca tcaactgcat caatggacac
    19921 gcctggtccg tttgaaaaaa aggcaacaga agcgtattat tatatcacac ctgtcaatct
    19981 tgcgtggaaa ccagagcaac aagaagattg gctgatgcaa tttaattact acggcacaga
    20041 cattgtttct attcacgaag cctatccagg acattataca cagttcttgc atttgaatgc
    20101 ctcagatgca tcgaaagtgc agaaaatttt cggcagctat gctttcatcg aaggttgggc
    20161 acattatacc gaacaaatga tgatagaaga aggttttgaa caaaaaggag attcgataaa
    20221 agcagctaaa tatcgattag cacaatcagg tgaagcatta ttgcgcttat gcagattggt
    20281 tgtttccatt cgtacacact gcaaaggaat gactgtagag gaagccacta cattttttat
    20341 ggacaattgg catcaaggag aaaaatcatc gcaacaagag gcattgcgtg gcacatttga
    20401 tccaggttat ttattctata ctttaggaaa attgcaaata ttgaaattga aagaagatta
    20461 taaaaaacaa gaaggaaaaa attattccat tcaaaaattt aatgatgcca tgacggataa
    20521 cggcatgcct cctattcgca tcatgcgtga aatattattg aaagatgaaa aaacttggaa
    20581 cgaaatattg taaaaaatat ttcgatggat aattacaaaa tctcatttac tttttctggc
    20641 ggtcgagcga cgactgcctt atctccatta ataacaattg gcctctctat caaaatagga
    20701 ttttcgttca ttgcctgtaa ccattcttca tcgctcaaat ttttgccttt atatttttcc
    20761 agataaatcg cttcttttgt gcgaatgata tcatgcggct tcgcttgtaa tttcttgagt
    20821 ataactttta attcttcaat tgatggtgtg tcttttaaat attcgacagt agtaaatggc
    20881 ttatggcttt cttctaaaat acataatgca cttctacttt ttccgcatct attattatgg
    20941 taaatcgtaa tcataaaaac tgtttaataa aactaaatgc ctgcgtaatt aaatggtgtt
    21001 atttttttca attcctcttt tataggatcg gcaacaagta aggcatctat aaaatcgtgt
    21061 atttcttttt cgccaatatg tgtattggtt ctggttaaat tttttagtgc ttcataaggc
    21121 ttatcaaaac cttcgcgacg taaaacagtt tgaatcgctt ctgcaacgac aatccaattt
    21181 tgttctaaat cttgttgaat tttttgttcg tttaccaata gcttattcaa gccttttaca
    21241 atcgaatcga aagcaataag cgtatgagcc aatggcacac caatatttct taaaacagtt
    21301 gaatccgtca aatcgcgttg taaacgtgaa atcggcaatt tttctgctaa atgtgtaaac
    21361 aaagcattgg caattcctaa attgccttcc gcgttttcaa agtcaatcgg atttacttta
    21421 tgtggcattg ccgagctacc tatttcacca gcttttattt tttgtttaaa a
//
LOCUS       DAOQQV010000033        21398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_734_length_21398_cov_8.193178, whole genome shotgun sequence.
ACCESSION   DAOQQV010000033 DAOQQV010000000
VERSION     DAOQQV010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21398
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_734_length_21398_cov_8.193178"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLL59_05215"
     CDS             complement(<1..296)
                     /locus_tag="PLL59_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23239.1"
                     /translation="MNKKMTLLVAMFSLIVSNLVAQISITNSNPYTQDFNSLPNSGSP
                     TTTNPNPVDWYRHAGTTNAINLTVGTGSSNSGGFYSVGATDSTDRALGSLLSSTP"
     gene            complement(469..2181)
                     /locus_tag="PLL59_05220"
     CDS             complement(469..2181)
                     /locus_tag="PLL59_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23240.1"
                     /translation="MKKMIFISIILLFFSFSISKAQQACLARTAAHIRLVGDSWAHFP
                     AIYAAYDSALAKYGFADYSTVSDGSVLISMTAETWWQNPLARFALETSLNTDAVRPID
                     IIIVSLGGNDVAFGIRKNDSLNVLDDNLFQARMYMDSIFDFIHAKYPNGQIIWQSYDY
                     PNFNDPCLNLPWNPYCDLWNGRGNPSPYEINRFMAYMTDFQDTVVQGYRSRGKEYMHF
                     YNNLGLMQWRYGQLTPLRFDPFDTYPPRTVPLPYGNMYYPSPLPAMGLLQNDTYHLSP
                     QGYTYLAEAYMRQFISNYFRKGRDTSIYSMGQNYDGWSDANGGSGIGEVLVGKQNPSL
                     DTKGIFSFNTEFIPDEKVIKKASLFFKLKTIKHAYRLGLVFPQHFTLDIKSGTFGNAE
                     IEGTDYSAVNTATDVACFAGKLIGNDYALRADLLPSALQYINKNGITQFSLSTADSNL
                     LSFFNGDTTAFEGPYLDIYYDSISVITKINQQKIDNKMLSVFPNPTSNKITLQLDNTW
                     LHQNILLEIYDSKGILVQTTNELNTSNEQIQLDVSQLAAGGYFISIKTDKMQSVATFI
                     KIDE"
     gene            2310..3200
                     /locus_tag="PLL59_05225"
     CDS             2310..3200
                     /locus_tag="PLL59_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23241.1"
                     /translation="MKTKLLLLFCCYVMLQNNIFCKNINFAEALKSATIKSSIKGNDM
                     STHYLEPIIISISNLGKDALEIAINTGDYFIPKEADKQNIIVTQTEIIALKAGESKNI
                     KIKGMCTEPADASGDASTVYTYKAIDNEKMKALSAFIAKNNYQSAAAQQAMWCLVDNR
                     DLNAIIAADSSEQNNLKRFMAALTGRTFTITTDDYQTNYYMPPKERVGGKFEYSFNKA
                     QDIQIAMFNSNGILVRELFNQKKVPSGTHQFTFEYDSSVYTDDTYYFKLIADNDVMVN
                     QKWEAKTMRDSFKKNIEDKY"
     gene            3266..3661
                     /locus_tag="PLL59_05230"
     CDS             3266..3661
                     /locus_tag="PLL59_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HMY23242.1"
                     /translation="MKSEPDVFSYDDLVNKKREHWDGVRNYTARNNMRAMKKGDIALF
                     YHSNIDKAVVGIMKIVKEAYQDPTTEETAWVCVDVAPKEKLKNPVSLASIKSNPKLAN
                     MKLIKQGRLSISDITKEEFDEILNMSKQA"
     gene            complement(3684..5042)
                     /locus_tag="PLL59_05235"
     CDS             complement(3684..5042)
                     /locus_tag="PLL59_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMY23243.1"
                     /translation="MSQEQEEVSFIRKIAFAAGQIGWSLAVFGFTELIYFFYLPPDNG
                     EPLFRAFVYQGPVFKIFTIVGILSALGYIISAFMEPVVGSLSDRSTFKFGKRKTFMVF
                     ALFPFAFLSAAVFFPLKDGIHSLNIVWLSVTVLSAFVIKCFYTTPYNAMINEIASKEK
                     ERLHILMMLSIAYALGIGIGQTINIVMSAFKDTLPNETIFQYCVLVYAFVAMLLMLMP
                     ILFVPDNSGKNISTAEEQYDKKHNSVLSTMQEVWSDKNYRVALIVELIYWFPQKVFVV
                     AVPYFVAALLQLDNVYTAIILYSVGIFSLFLYPLVDKLVDKYGKKKIMQSAFFCLIFG
                     FLFTSTIGLYKLPAQLFIGIYVILNVYPTAVLGILPMAIAGDNAQVNFQQTGVAKNAS
                     YYALKTFMMKIGVAITSLIFPSLLLLGKTPENPLGVRLVAVASLICSLLAMIVMRKYK
                     DV"
     gene            complement(5146..6042)
                     /locus_tag="PLL59_05240"
     CDS             complement(5146..6042)
                     /locus_tag="PLL59_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23244.1"
                     /translation="MPKSQALLKIESLHPEKDAWEIIRLSLFYEFPWDFNRALEFALY
                     KTYATPSVVNVLHRTKELENHTQKRYDDTDILLSEIIENGLKEGRGKQALEQMNKIHA
                     AFKISNDDYIYVLSTFVFDTAFWINKYGYRKLTSNEELAGYYVWKEIGEHMHIHSIPD
                     SIFKLKKFHDDYEAQNFIYTEVNAKVAKATENLMLSWVLPSSLFELGRPVLHAVMEPH
                     LLKAFNYQEPPKILRQSIANLLRIRSSVESWFPRKSPYIRTKDYKHKAYPNGYTIEQL
                     GPEKIINAAACPYHAVLNAVKQ"
     gene            complement(6160..6795)
                     /locus_tag="PLL59_05245"
     CDS             complement(6160..6795)
                     /locus_tag="PLL59_05245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMY23245.1"
                     /translation="MSLRLGDEAPNFTAETTEGTINFHEWLGNNWAVLFSHPADYTPV
                     CTTELGYTAKLKDEFSKRNVKVLALSVDDIDSHHGWIKDIEETQHVAMNFPIIADPDK
                     TVANLYDMMHPNSSEKFTVRSVFVIGPDKKIKLTITYPASTGRNFNEILRVIDSLQLT
                     ANYSVATPVNWQEGEEVVIAPTISNEEATTKFPKGFKIIKPYLRLTPQPNK"
     gene            6906..7331
                     /locus_tag="PLL59_05250"
     CDS             6906..7331
                     /locus_tag="PLL59_05250"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMY23246.1"
                     /translation="MNSIFISQTFNAPQDEIFAYLCDHNNLGAILGAQILRIKDADSG
                     NPNGVGSVRSIKIGVELLQETVVTFDEPDLIEYKISSNAPINYHLGCMEFSTPAPGKT
                     QLNYTIDLESKIGIIDGAVGFILKTVITNGLKKLAAKYN"
     gene            7331..7801
                     /locus_tag="PLL59_05255"
     CDS             7331..7801
                     /locus_tag="PLL59_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMY23247.1"
                     /translation="MLGLRTRPEKQKLHFLQDFNAPVKNVFAFFSDHNRLKEIYPGFI
                     KRIIDSPNPANCNDVGSSRVIFSFPLLFQETTTQYIESKYLEYKITFGSPIKNHIGKM
                     HFIELSPTKTRLDYIIEFEPVIPYTGFIIQQINSKIVQDALSTLSLRFNQNPNY"
     gene            7798..8259
                     /locus_tag="PLL59_05260"
     CDS             7798..8259
                     /locus_tag="PLL59_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23248.1"
                     /translation="MNKLHLFIFVACITLFSCKKDEFIQVDYIVFGAIQGDCPNGCRY
                     VYYLDSQKLLEDEQVKYYANQQLSNFNKALTGEQFELVKTLLHQVPSAMTKTSKSLFI
                     DLNAGSPNLWYAEVKANNRIYKWTFDNAPSSTPAYLQTFANAVINATQQLQ"
     gene            complement(8564..9004)
                     /locus_tag="PLL59_05265"
     CDS             complement(8564..9004)
                     /locus_tag="PLL59_05265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY23249.1"
                     /translation="MNDVEIQIVPFGSTIYEQTITLRDDVLRKPIGLAYTPEQLALEH
                     DEVHIAAVLNGNVIGVLLLRKGEYRVLKMRQVAVATTFQRCGIGRKLVVFAEKYAKEN
                     NFDAIELHARESALAFYKSLHYTQIGDEFTEVGLPHFKMKKLIL"
     gene            9107..9691
                     /locus_tag="PLL59_05270"
     CDS             9107..9691
                     /locus_tag="PLL59_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23250.1"
                     /translation="MREQNITELESLAIIQEMIEKTRQHLNDKSTYFFLWGFAVFLCA
                     IAQYIMLKMLLTNTQRVWLAMPIVAMVHMYLVIKEQKKSVVKTHNDTAIQSLWIALGI
                     GFIVLTYLSFRISFNIFPLLILLYGIGTFVTGRIIQFTPLWLGGIACFILSVAITFID
                     GSEQLLILALAVLLSYIIPGFLLKLQYKNQQKAV"
     gene            9691..9987
                     /locus_tag="PLL59_05275"
     CDS             9691..9987
                     /locus_tag="PLL59_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMY23251.1"
                     /translation="MKAFANLDPLLHSQLRLAIISLLVAVKEADFGYIQEATQSTNGN
                     LSVQLSKLKDAEYIEVRKSFENNYPKTTCKITKKGIKAFEKYVEDIKQYLSPKK"
     gene            9993..10262
                     /locus_tag="PLL59_05280"
     CDS             9993..10262
                     /locus_tag="PLL59_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HMY23252.1"
                     /translation="MENKDEKLWQIATQRAKFRREIISYVVVNLFLWCIWYFTKAQAP
                     SSYIPWPAWVSLGWGFGLIMKYFSVYHFSNEDQIQKEYDKLTNKD"
     gene            10439..11065
                     /locus_tag="PLL59_05285"
     CDS             10439..11065
                     /locus_tag="PLL59_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HMY23253.1"
                     /translation="MKIEQVFKNNEKWIASKLAIDEHYFKKLSKGQNPELLYIGCSDS
                     RVSAEELMGVQPGEVFVHRNIANLVNNIDLNVMSVLNFAIRQLKVNHIVVCGHYNCGG
                     VKAAMQPEDLGILNPWLRNIRDVYRLHNEELNDIKDIEKRYNRLVELNVQEQCVNLIK
                     TAAVQQAYKERGLSVHGWVFDMHTGKLIDLEINFPKILKNIMEIYALK"
     gene            11223..11843
                     /locus_tag="PLL59_05290"
     CDS             11223..11843
                     /locus_tag="PLL59_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23254.1"
                     /translation="MYASGSFGADTTFFSYNTEKKIIAATTSSSSNTYIYSGNNYVRK
                     NTSGLFETRDSILIQKDNLIREIYSFNTANVLNRKQSYTYNSSDNLIQYTDQNFIPAG
                     VDIYNIDYADGDVISLNKGITPTATFTYYTDKSAAKGDYLDIFQFINFGFGKYLKSKH
                     LIKTMKNGSAATVNISYEFDSHGNVSKLTASDGVNTDTYTYTYTCD"
     gene            complement(11848..12990)
                     /locus_tag="PLL59_05295"
     CDS             complement(11848..12990)
                     /locus_tag="PLL59_05295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMY23255.1"
                     /translation="MQKVKKLFIALVVLAAIYFVLPYHVQMGLKHTFANVDDYKLFPS
                     HKLSKSSTPIAWQLSENYNKKAPSAELLNFFDERQTCAYLVIQDGKIVYERYAQAYTK
                     ETISGSFSMAKTVNALLIGKLIELGKIKSINDDVKDYVPELTQIPKGKLTIKDVITMS
                     ADIDWHESYWNIFSLTAESYYGDDLNALLVKLKIRKDEQQGKLFEYQSCCTQILGYII
                     KNATKKTIAENANEYLWQPLGAESDALWSTDKNGIEKSFCCLNATARDFAKLGQMVLQ
                     HGKWNQQQIIDSSYLAALTSPATYLKDTKGNTCDWYGYQTWIINYEQHQIPYFRGILG
                     QFIFMIPDKNAVIVRLGKKIKKDEDDPKYQNDDIRNYIKAGLEVLQ"
     gene            complement(13061..14203)
                     /locus_tag="PLL59_05300"
     CDS             complement(13061..14203)
                     /locus_tag="PLL59_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23256.1"
                     /translation="MRNVCSILMFCLFITSSIFSIAQEATFSKGSKVKILEIDKSDAY
                     YSERSDFVGNDATVKSDLTKDSKGFYSGTLETSSGRVCYFSKVKLTQVASSKTALLDK
                     TSSIKFITGTIKKGTIVYVADISKEDSYYSDRHEHIGKKGKVTENDMEMKEDGYYAGD
                     FAYDDGKEVYFYKAKFSKDPVPAVEKSADDVDDTNASSEVYDWLGGDDKVTDESWNDA
                     RNDDDIKEGDKVEITAISPEDGFYDDRADYIGKQGIAGDDIDFQSDEGGYSGTVKLSN
                     DKDDVYFYLVKLKKVSSSNTSSKIAAADVKSSSSATKISKGTKVMIMSLSPDDSYYIN
                     KEDYLRKKGTVTEGLDIDEEGYYSGNIKMDGGAKSYFYKVKVKVIQ"
     gene            complement(14300..15247)
                     /gene="nusB"
                     /locus_tag="PLL59_05305"
     CDS             complement(14300..15247)
                     /gene="nusB"
                     /locus_tag="PLL59_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HMY23257.1"
                     /translation="MITRRSVRIKTMQYVYAYESTASAASSQFEQYLEKSILSVKDQY
                     LYLLLCIREVSNFVEVDAKIKAGKFIKQDSDKNFNTKLLSNVIIQYLNNDKAFLAEIS
                     QAKTAQLLDEDTIRQWYKIFIATPEYKAYLENGTEFNVDADRAIVKFLLDAVLLEDEN
                     FNTIIEELFTNWADDAEYVVDAVHEVIQKSKNELKLHLEKGNLKEKFRELKQFGIDLF
                     RITIQNKKDHQVIIKPKLKNWESDRLAMLDVILIRMALAEFLDFPSIPVKVTINEYLD
                     IAKEYSTPKSKDFINGILDSIMKDLKSNNTLNKTGRGLL"
     gene            15409..17220
                     /locus_tag="PLL59_05310"
     CDS             15409..17220
                     /locus_tag="PLL59_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMY23258.1"
                     /translation="MKELYAVNKYFIKYKWLLLLGVLFVISSNIFGLYTPSYIRYAVD
                     IVFENIALYKLTDGFAKQAILKTYFNYFIMYFACIILITSIIKGLLMFFMRQTLIVMS
                     RNVEYDQKNELYQKYQKLNTTFYKRNNTGDLMSRISEDVSRVRMYIGPAIMYFINTGA
                     LFLMVIYMMFNINAYLSFLVLLPLPILVYSIYKVSDIINKKSEQISVALSGLTSQAQE
                     VYSGIRIIQSFGIHQQVQQDFHDASSAYRQKNISLAKVDSFFAPLMLLLIGLSTLLVV
                     YIGGKEVVNGNFTAGNIAEFIFYINMLTWPVASLGWCVSLVQRAEASQKRINQFLKDD
                     NEIVNTAQYPIKSIDSIKFNNATFIYPDTGIQAISDLSFTINKGEKVAIIGKTGSGKS
                     TIAELLLRNYDIESGSISINQHNIKQIDLHEYRGKIGYTPQDVFLFSDTIKNNIVFAN
                     QIAENEEILLEKAIQFAKVTHVHHDIMLLKQQYNTIVGERGVMLSGGQKQRISIARSL
                     FNNPEFVLLDDCLSAVDAKTEKTILHNLQASLKDKTVLFITHRVSAIMHFDKILVIDE
                     GKLVEQGRHEELLRNKSIYYDIYQLQRQKNDLKNSNM"
     gene            17313..17735
                     /locus_tag="PLL59_05315"
     CDS             17313..17735
                     /locus_tag="PLL59_05315"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HMY23259.1"
                     /translation="MENEKNFDKNGVYSKRVRAGKRRTYFFDVRTTRGNDYYLTITES
                     KKRADDDTYERHKIFIYKEDFMKFVNAMQETVDYIKTDLLPDYDFDQHNHDYDEDGNY
                     QRNEDKTENEDVSAPIKTSETPSSDFKAQQSSEEDKWD"
     gene            complement(17790..18335)
                     /locus_tag="PLL59_05320"
     CDS             complement(17790..18335)
                     /locus_tag="PLL59_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23260.1"
                     /translation="MLVILINSCKKSEPDDPNIIYKKIDKSVKAVFANLPKVDSFDLN
                     DDAHIDFKIAAISNAAADTAVTIIFGESAAVYCDSAFVYSSFFFKVKNLYEQEIPDIY
                     NGSKRWWDNVYVTQRSGSNKTGYAGVGDVYLPIIITISSPTDFHYGWLRVQAESDYRS
                     LTVIDGAYHVQANVPIKMGAK"
     gene            18509..19867
                     /gene="mgtE"
                     /locus_tag="PLL59_05325"
     CDS             18509..19867
                     /gene="mgtE"
                     /locus_tag="PLL59_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HMY23261.1"
                     /translation="MEVSKEELIIDELKMENPVFTDFRTSELADFLNDSPEYSNKIIM
                     ALEPHRAVKTMKFLDIKLQKKLIHTLPSNNVATLIQNMHFDDRTALLSELTSESVKKL
                     VLFLPHEDRIETLALLGYQEDSVGRLMNPDYIEVKSHYTVQDALNKIRRLGRTIENIN
                     FIYIIDENEKLIDDINLKELLITSPDTKISALMDTKYLALHVNDDQETAASVFRKNNR
                     AVLPVTDDNGVLLGIVTIDDILRVESEEATEDIQKIGGTEALDEPYMNTPFFDLIKKR
                     APWLVILFIGEMLTASAMAFFEDEIAKAIVLVLFIPLIISSGGNSGSQASTLIIRAMA
                     LGEITFEDWWRVMRREIMSGIVLGSILGFIGFLRIIIWNAVNPIYGEHYILVGITVGV
                     TLIFIVLWGTLCGAMLPIVIKKLGFDPATSSAPFVATLVDVTGLVIYFIVASLLLSGS
                     LL"
     gene            20001..21101
                     /gene="ychF"
                     /locus_tag="PLL59_05330"
     CDS             20001..21101
                     /gene="ychF"
                     /locus_tag="PLL59_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMY23262.1"
                     /translation="MGLQCGIVGLPNVGKSTLFNAVSNNAKAQASNYRFCTIEPNVGL
                     VDVPDERLQKLAEIVVPNRTVSTTIEFVDIAGLVRGASKGEGLGNKFLGNIREVDAII
                     HVIRCFEDDNILREEGAINPIGDKDIIDTELQLKDLESVEKKIAKIQKQAKVGGDAKA
                     KHEYDVLLQCQQCLNDGKNIRSLQFSKDDRALFADLCFLTDKPVLYVANVDEASILTG
                     NKYSEALTQMAKEENALVIVLNNSVEAQISEMEEDDKALFQSEYGLTEPGLNRLIRSA
                     YDLLQLQTYFTAGVQEVRAWTIHKGWKAPQAASVIHTDFEKGFIKAEVIAYEDFIHYK
                     SEAACRENGKLRIEGKEYIVKDGDVMHFRFNV"
     gene            21180..21344
                     /locus_tag="PLL59_05335"
     CDS             21180..21344
                     /locus_tag="PLL59_05335"
                     /inference="COORDINATES: protein motif:HMM:NF028380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HMY23263.1"
                     /translation="MGRGDKKTRKGKIFAGSFGKSRPHKVKAKATPKKEVTETKAKKG
                     APKKTKAAAE"
BASE COUNT         7416 a         3590 c         3501 g         6891 t
ORIGIN      
        1 ggcgtggacg acaataaact gcctaatgct ctgtctgtag aatcagttgc acctacagaa
       61 tagaaaccac ctgaattgct agagcctgtg ccaacagtaa gatttatagc atttgttgta
      121 ccagcatgtc tataccaatc aactggattt ggatttgttg ttgttggcga accagagttc
      181 ggtaggctgt taaaatcctg cgtataagga tttgaattgg taatcgaaat ttgtgctact
      241 aggttgctca cgattaagct aaacatagca acaagaagtg tcatcttttt attcatcttg
      301 taaaatttta tcataaatta ttgtacaaaa atacataata tttcgttcca tagttctgat
      361 ttgacagcaa agtaagaaat tgtggataat aaaattaaac aagcacataa atcgattgca
      421 aatgtgttaa aatattgatt taaaacaagt taaatttcaa tttcttatct attcatcaat
      481 ttttataaaa gtagcgactg attgcatttt atctgttttt atactaataa aatatccacc
      541 tgcggcaagt tggctcacat caagttgtat ctgttcattt gatgtattga gttcatttgt
      601 cgtttgaact aagatacctt tgctatcata gatttccaat aggatatttt gatgcagcca
      661 agtattatcc aattgcaatg ttatcttatt actagtagga tttgggaaga cagacaacat
      721 tttattgtct attttttgtt gattgatttt tgtaatcacg cttattgaat cgtaataaat
      781 atctaaatac gggccttcaa atgctgtagt atcgccatta aagaaggaca acaaattaga
      841 atctgctgtg cttaagctaa attgagtaat gccattttta ttgatatatt gcaaagccga
      901 tggcaacaag tcagcacgca aagcgtaatc gttaccgata agctttccag caaagcaagc
      961 tacatcagtg gccgtgttta ctgctgaata atctgttcct tcaatttctg catttccgaa
     1021 agtacctgat ttaatatcta aggtaaaatg ctgtggaaat actaagccta agcgatatgc
     1081 atgtttaatt gtttttaatt taaaaaacaa agaagctttt ttaattactt tttcatcagg
     1141 aataaattct gtattaaaac taaaaattcc ttttgtatcc aatgaaggat tctgtttgcc
     1201 aacaagcact tcaccaatac cacttccacc gttggcatct gaccagccat cgtaattttg
     1261 acccattgaa tatatactcg tgtctctccc ttttctaaaa tagttgctga taaattggcg
     1321 catgtatgct tccgctaaat aagtataacc ttgcgggctt aaatgataag tatcgttttg
     1381 caacaaaccc atggctggca gcggcgacgg ataatacata ttaccataag gcaatggcac
     1441 tgtacgtggt ggataagtat cgaaaggatc aaaacggagt ggtgtaagct gaccataacg
     1501 ccattgcatt aatccaagat tattataaaa atgcatgtac tcttttcctc tacttctata
     1561 accttgtact acggtgtctt gaaagtcggt catatatgcc ataaagcgat tgatttcata
     1621 cggcgacgga ttgccgcgtc cattccataa atcacaataa ggattccaag gtaagtttag
     1681 gcaaggatca ttaaagtttg gataatcgta gctttgccat ataatttgac catttggata
     1741 tttagcatga ataaaatcaa agatactatc catatacata cgagcttgaa ataaattatc
     1801 atctaaaaca tttaaagaat cattctttcg aataccaaag gcaacatcat ttccgcctaa
     1861 ggatacaata ataatatcta tcggtcgaac agcatcagtg tttaatgatg tttccagtgc
     1921 aaatcgtgct aaaggatttt gccaccaagt ttctgccgtc atgctgatta atacgcttcc
     1981 atcagaaact gtcgagtaat cagcaaatcc atattttgcc aatgcggaat cgtaagcagc
     2041 atatattgca ggaaaatgag cccagctatc tcctaccaag cgaatgtgcg cagctgtgcg
     2101 agcaagacaa gcctgttgtg ctttacttat agaaaaacta aaaaataata gtattataga
     2161 aatgaaaatc atctttttca tagatataac tggttatggt ttagaataca attatttgtt
     2221 ttgactacaa ataagccaat tgaagataag aaaaaaaatt gtgaattaaa atatgtattt
     2281 ataatttgcg tttataaata aactcaataa tgaaaaccaa actcctattg ctattttgtt
     2341 gttacgtaat gttacaaaac aacatatttt gcaagaatat taattttgct gaagcattaa
     2401 aaagtgcgac aataaaatca agtataaaag gtaatgacat gagcacacat tatctagagc
     2461 caattattat tagcatcagt aatttgggaa aagatgcttt ggaaattgca ataaacacag
     2521 gcgactattt tattccaaag gaagcagata aacaaaatat tattgttacg caaacagaaa
     2581 ttattgcttt aaaagctggc gaatcgaaaa acattaagat aaaaggaatg tgtactgaac
     2641 cagcagatgc gagtggtgac gctagtactg tatatactta taaagctatt gacaatgaaa
     2701 aaatgaaggc actttctgcg tttattgcaa aaaataatta tcaatcagca gctgcgcaac
     2761 aagccatgtg gtgtttagtt gataatagag atttaaacgc aatcattgca gcagatagta
     2821 gcgaacaaaa taatcttaaa cgttttatgg ctgcattaac aggcagaaca tttacaataa
     2881 caactgatga ttatcagact aattattaca tgccaccaaa agaaagagtt ggcggcaagt
     2941 ttgaatatag tttcaacaaa gcgcaagata ttcaaattgc aatgtttaat agcaatggca
     3001 ttttagtgcg cgaattattt aatcaaaaga aagtaccgag tggcacacat cagtttacat
     3061 tcgaatacga ttcttcagta tatactgatg atacttatta cttcaaatta attgctgata
     3121 acgatgtaat ggtcaaccag aaatgggaag ctaaaacgat gcgcgatagc ttcaaaaaaa
     3181 atatagaaga caaatattag ctatttgaaa tctactgcat actgcttact tttatccaaa
     3241 attattgaag gtgaattatt ggttaatgaa atcagagcca gatgtttttt cttatgatga
     3301 tttggtcaat aagaaaagag aacattggga cggcgtgcga aactacactg cacgaaataa
     3361 tatgcgagcg atgaaaaaag gtgacattgc cttattttat catagcaata tcgacaaagc
     3421 cgttgttggc atcatgaaaa ttgtaaaaga agcctatcaa gatccaacaa cagaagaaac
     3481 agcttgggtt tgcgtagatg tagcaccaaa agaaaaatta aaaaatccag taagtttagc
     3541 tagcataaaa tccaatccta aattggcaaa tatgaaatta ataaaacaag gaagattatc
     3601 aatttcagat attacaaaag aagagtttga cgagatatta aatatgagta agcaagcata
     3661 gaaatgcaat ttacaatgcc attttaaaca tctttatatt ttctcattac tatcatggcc
     3721 aacaagctac atatcagact tgcgacagcc actaagcgca cacccaaagg gttctcaggt
     3781 gttttgccta ataacagtaa agatgggaaa attaaactgg taatagcaac gccaattttc
     3841 atcataaatg ttttgagcgc ataataggat gcatttttag cgacgccagt ttgttgaaaa
     3901 tttacttgtg cattgtcacc tgcaattgcc ataggcaaaa tacctaagac cgcagttgga
     3961 tatacattta atatcacata aatgccaata aaaagttgag caggcaattt atacaaaccg
     4021 atagttgatg taaataggaa accgaaaatc aaacaaaaaa atgcggattg cataattttt
     4081 ttctttccat atttatctac aagtttatca actaatggat acaaaaacaa agaaaatata
     4141 ccaacgctat ataagataat tgccgtgtac acattgtcta attgcaagag tgctgccacg
     4201 aaataaggca cagccacgac aaatactttt tgcggaaacc aataaatcag ctcaacaatt
     4261 aaagcaactc tataattttt atcgctccat acttcttgca tcgttgacaa cacactattg
     4321 tgttttttat cgtattgctc ttctgctgtc gaaatatttt ttccagaatt atctggcaca
     4381 aaaagtatgg gcatcaacat gagtaacatg gcaacaaacg catacactaa aacacaatat
     4441 tgaaaaatag tttcatttgg taatgtatct ttaaatgcac tcattactat atttattgtt
     4501 tgcccaattc caatacctaa agcataagca atcgacaaca tcatcaatat atgtaagcgt
     4561 tctttttcct ttgaagctat ttcatttatc attgcattgt atggtgttgt ataaaaacat
     4621 ttaatgacaa atgccgacaa tactgttact gacaaccata caatattcaa tgaatgaatt
     4681 ccatctttta atggaaaaaa aactgcggca gataaaaagg caaatggaaa aagagcaaaa
     4741 accataaatg tttttcgctt tccaaatttg aatgtacttc tatcgctaag gctaccaaca
     4801 acaggctcca taaaggcact aataatataa cctaatgccg acaaaatgcc gacgattgta
     4861 aatattttaa aaactggacc ttgatataca aaagctctaa ataatggctc accattatct
     4921 ggtggtaagt aaaagaaata aatcaactcg gtaaatccaa agaccgctaa actccaccca
     4981 atctgtcctg ctgcgaatgc tatttttcta ataaaggaaa cttcttcctg ttcttgactc
     5041 atgtagcgaa gatacattaa ttctaaaaaa ttacatgaac accttgcgta ttttgtaaga
     5101 ataaatcagc acaaccttaa attctgattt taagtcacat tgaaattatt gcttaaccgc
     5161 attcagcaca gcatgatatg gacaagcagc agcattgatg attttttccg gacctaattg
     5221 ttcaatcgta tagccatttg gataggcttt gtgcttgtag tctttggttc tgatatatgg
     5281 tgatttccta ggaaaccagc tttcaacgga actgcgaata cgcaataaat tagcaataga
     5341 ttgtcttaat atttttggtg gttcttggta gttaaatgct tttaataaat gcggttccat
     5401 cacagcatgt aaaacaggcc tgcctaattc aaataaagat gaaggcagca cccagcttaa
     5461 cattagattt tctgttgctt tggccacctt tgcatttact tctgtgtaaa taaaattttg
     5521 tgcttcgtaa tcgtcatgaa attttttcaa tttaaaaata gaatcaggaa tagaatgtat
     5581 atgcatgtgc tctccaattt ctttccaaac ataatagcct gccaattctt cattactcgt
     5641 caacttgcga tagccatatt tattaatcca aaaagcagta tcaaatacaa atgtgctgag
     5701 cacataaata taatcatcgt ttgatatttt aaatgctgca tgaattttat tcatctgctc
     5761 taaagcctgt ttacctcttc cctcttttaa tccattttca ataatttcac ttaataaaat
     5821 atctgtgtcg tcatatcttt tttgtgtatg attttctaat tctttagttc gatgcaaaac
     5881 atttacaaca gatggcgttg catacgtttt gtacaaagcg aattcgagtg cacgattaaa
     5941 atcccatgga aattcgtaga acaaggacaa gcgaattatt tcccaagcat ctttttctgg
     6001 atgcaaagat tctattttta acaaagcctg tgattttggc ataactaaat ggtttatact
     6061 aaaatataca aaatttctaa ttaaagcaaa taagaatgac acgtaagaag aaataaaaaa
     6121 ggtcaacaca atggttgacc tataacacgt aaattgtaat tacttatttg gttgaggtgt
     6181 taatcgcaaa tagggtttga taatcttaaa accttttgga aattttgttg tcgcttcctc
     6241 attagaaatt gttggtgcaa ttactacctc ttctccttct tgccaattaa ctggtgtagc
     6301 tacgctgtag ttagctgtta attgtaaaga atcaataacg cgtaaaatct cgttaaaatt
     6361 tctacctgtt gacgctggat aggtaattgt cagtttgatt tttttatcag gaccgatgac
     6421 aaaaacacta cgcactgtaa atttttctga agaatttggg tgcatcatat catacaagtt
     6481 ggccaccgtt ttgtctggat cggcgatgat gggaaaattc attgctacat gttgtgtttc
     6541 ctcgatgtct tttatccagc cgtgatgaga gtcgatatca tcaacactca aggctaagac
     6601 ttttacattt cgcttcgaaa attcatcttt taattttgcg gtatagccta attcggtagt
     6661 gcatactggt gtataatctg ctggatgaga aaataaaact gcccaattat ttcctaacca
     6721 ttcatgaaag ttaattgttc cttctgttgt ttctgctgtg aagttgggcg cttcatcgcc
     6781 taatcgtaat gacataatat taaatttttg tgttttaaaa agcaatttcg gtttaaatat
     6841 acaattagtg gaaataaaac atttgttaaa aagtaaaaaa gagtaaattt atagaaacat
     6901 caaacatgaa ttcaatattt atatcacaga cgtttaatgc tccacaagat gagatttttg
     6961 cctatttatg cgaccacaat aatttaggtg ctattttagg agcgcaaatt ttacgcataa
     7021 aagatgcaga cagtggcaat ccaaatggtg ttggttctgt tagaagcata aaaataggtg
     7081 tcgaattatt gcaggaaact gtggttacgt ttgatgagcc tgatttaata gagtataaaa
     7141 taagcagtaa tgcacctatt aattaccatt taggttgcat ggaattttcg acaccagcac
     7201 caggaaaaac acagttaaat tatacaatcg atttggaaag taaaatcgga ataatagatg
     7261 gtgctgtcgg ttttattcta aaaaccgtaa ttactaatgg cttaaaaaaa ttagctgcta
     7321 aatacaacta atgttaggat taagaacaag acctgaaaaa caaaaattgc atttcttaca
     7381 agattttaat gcacctgtaa aaaatgtatt tgcttttttt agtgatcata atcgtttaaa
     7441 agaaatttat cctggattta taaaacgcat tattgattcg ccaaatcctg caaactgtaa
     7501 tgacgtcggt tcgtcgcgag tgatttttag ttttccttta ttatttcaag aaacaacaac
     7561 acaatatata gaatcaaaat atttagaata taaaatcacc tttggcagcc caataaaaaa
     7621 ccacattggc aaaatgcact ttattgaatt atcgccaaca aaaacaagac ttgattatat
     7681 tatagaattt gaacctgtga taccttatac tggatttatt atacaacaaa tcaattctaa
     7741 aattgtacaa gatgcactaa gtactttatc tttaagattc aatcaaaatc caaactattg
     7801 aacaaactac atctatttat ttttgtcgca tgcattacac ttttttcttg taaaaaagat
     7861 gaatttatac aagtagatta cattgtgttt ggtgctattc aaggagattg tcctaatggt
     7921 tgcagatatg tatattattt agattctcaa aaattattgg aagatgaaca agtaaaatat
     7981 tatgccaatc aacagttatc gaatttcaat aaagcactta caggcgaaca atttgaactc
     8041 gtaaaaacgc ttttgcatca agttcctagc gcaatgacca aaacatcgaa atcattattt
     8101 atagatttga atgctggcag tccaaactta tggtatgctg aagtaaaagc aaacaacaga
     8161 atttataaat ggacttttga caatgcaccg tcgagcacac cagcttactt gcaaacattt
     8221 gccaatgcag ttatcaatgc tactcaacaa ttacaataaa tagcaatacg ctaaatagtg
     8281 aatggctaaa cataaagtct agccatttcc aaaattacaa tctgttagaa agttttattt
     8341 tcaataataa tatattggta tatcattagg tttcgaaata gcattttttg acggaaattt
     8401 tcgttttcgc acaattgcag gaactgcctt taaatttaat ccaagttgtg gggacacttg
     8461 attaaaaata cgcattacat acgtacataa tctaaataca aatatcatga ctgggttttt
     8521 atgtatttta aagttgtgta aaaaaaatga aaagcgcaat agtctacaat attaattttt
     8581 tcattttaaa atgtggcaat cctacttctg taaactcatc tcctatttgt gtataatgta
     8641 agcttttata gaatgccaat gcagattcgc gcgcatgtaa ttcgatagca tcaaaattat
     8701 tttctttagc atatttttca gcaaatacaa ccaacttacg accaatgcca catcgttgaa
     8761 atgttgtagc tacagcaact tgccgcattt tcaatactcg atattcacct ttacgtagca
     8821 ataagacacc aataacgttt ccatttaata ctgctgcaat atgcacttcg tcgtgctcta
     8881 atgctaattg ctcaggtgtg taagccaaac caataggctt gcgtaaaaca tcatctctta
     8941 atgtgattgt ttgctcataa attgtcgaac caaaaggtac aatttgtatt tccacatcat
     9001 tcatacatat aaaaatacag gattaaatgt taaaaattgg aactgatgtg aaaaatattt
     9061 ggtttatatc ataaactact ttatatttac acttaattta caaatcatga gagaacaaaa
     9121 tataacggaa ttagaaagtc ttgctattat tcaagagatg attgaaaaaa caaggcaaca
     9181 tttgaatgat aaaagcacat acttttttct ttggggtttt gccgtctttt tatgcgctat
     9241 tgctcaatat atcatgctaa aaatgcttct aacaaataca caacgtgtgt ggttggcaat
     9301 gccaattgtt gctatggtgc atatgtattt ggtgataaaa gaacaaaaga aaagtgttgt
     9361 aaaaacacat aatgacacag ccatacaatc cttatggatc gcactcggaa ttggatttat
     9421 tgttcttacc tatttgtctt tccgtatttc atttaatatt ttcccattgt tgatactatt
     9481 atatggtatt ggcacctttg ttacaggaag aatcatacaa tttacaccat tatggcttgg
     9541 tggtatcgct tgttttattc ttagcgttgc tattactttt atcgatgggt cggaacaatt
     9601 gctaattctt gctttggcgg ttttattgtc atatattatt ccaggatttt tattaaaact
     9661 acaatataaa aatcaacaaa aagctgtata atgaaagcat ttgccaactt agatccttta
     9721 ttacattcac aattgcgatt ggcaataatt tctttattag ttgctgttaa ggaagctgat
     9781 tttggttaca tacaagaagc tacacaatct acaaatggga atttaagtgt acaattatca
     9841 aaattaaaag atgcagaata tatagaggta aggaaatctt ttgaaaataa ttatccaaaa
     9901 accacatgca aaatcacaaa gaaaggcata aaagcatttg aaaaatatgt cgaagacatt
     9961 aaacaatatt tatcacctaa aaaataaaat ttatggaaaa caaagatgaa aaattgtggc
    10021 aaattgctac acaaagagca aaattcagaa gagagattat tagctatgtc gttgtcaacc
    10081 tattcttgtg gtgtatctgg tatttcacca aagcacaagc accatcaagc tatataccat
    10141 ggccggcatg ggtaagctta ggctggggct ttggcttgat tatgaaatat ttttctgtgt
    10201 atcatttttc caatgaagat cagattcaaa aagaatacga caaattaaca aacaaagatt
    10261 aaggagtaaa gaggcacaaa tgtgtctctt tttttataaa aaaagcttac ttttattagc
    10321 aaacaaacag ctgattaaaa ctgaacagcc tatatacaaa tataatgtag gcatagttta
    10381 caaaatatta ttaataaaca aaaatcaagt agtttactcg cttttatcaa accaaaaaat
    10441 gaaaatagaa caggtattta agaacaatga aaaatggata gccagtaaat tggcaattga
    10501 cgagcattat tttaaaaaat tatctaaagg tcaaaatcct gaattattat atatcggatg
    10561 ctctgatagt agagtatctg cggaggaatt aatgggcgta caaccaggtg aagtgtttgt
    10621 acatagaaac attgcaaact tggtaaacaa tatcgattta aatgtgatgt ctgtattgaa
    10681 ctttgctatt cgccaattga aagtcaatca cattgtcgtt tgtggtcatt ataattgtgg
    10741 aggcgtaaaa gcagccatgc agccagaaga tttaggcatt ttaaatccat ggttgcgaaa
    10801 tattcgcgat gtatatcgct tgcataatga ggaattaaat gatatcaagg atattgaaaa
    10861 gcgatacaat agacttgttg aattaaatgt gcaagagcaa tgtgtcaacc ttataaaaac
    10921 agcagctgta caacaagcat acaaagaaag aggcttgagt gtacatggtt gggtttttga
    10981 catgcatact ggaaagctaa tagacttaga aataaatttc cctaagattt tgaagaatat
    11041 catggaaata tacgctttaa agtagcgata tatcttcttt tatattaatt tagttgtata
    11101 aaataggata aactatgaaa aaatacgctt taaaatccag cttaatttta gtactattta
    11161 cttgtgtact atcaattgtt tcttgtaaaa aaaattctca aaatgaatgc ttattaaaag
    11221 tcatgtacgc aagtggtagc tttggtgctg acactacctt ttttagttac aatactgaaa
    11281 agaaaattat agcagcaaca acgtccagtt caagcaatac ctatatttat tctggaaata
    11341 attatgtacg caaaaatact agcggtcttt ttgaaacgag agattcaatt ttaatccaaa
    11401 aagacaattt aataagagag atttattcat ttaatactgc taatgtatta aacagaaaac
    11461 aatcctacac gtataatagt agtgataatt taattcaata cactgatcaa aattttattc
    11521 ctgctggagt tgatatatat aatatcgatt atgcagatgg tgatgttatt agccttaata
    11581 aaggtattac acctacagct acatttacat attatactga caaatcagct gcaaaaggcg
    11641 attacttaga catctttcaa tttatcaatt ttggatttgg aaaatatctt aagtccaaac
    11701 atctcataaa aactatgaaa aatggaagtg ctgccactgt aaacattagc tatgaatttg
    11761 atagtcatgg taatgtaagc aaattgactg caagcgatgg cgtcaacaca gatacttata
    11821 cttataccta cacttgtgac taatttttta ttgcaaaact tctaaacctg ctttaatata
    11881 atttctgata tcatcatttt gatatttagg atcatcttca tcttttttaa tcttttttcc
    11941 taagcggaca atgacagcat ttttatctgg aatcataaaa ataaactgac ctaagatgcc
    12001 tctgaaataa ggaatttggt gttgctcgta attaattatc catgtttgat atccatacca
    12061 atcacaagta tttccttttg tatcttttaa ataagtagct ggcgatgtta atgccgccaa
    12121 atagcttgaa tcaataattt gttgttgatt ccacttacca tgttgcaaca ccatttgacc
    12181 taattttgca aagtcgcgag ctgtcgcatt taggcaacaa aatgatttct cgatgccatt
    12241 tttatctgtg ctccaaagcg catcactctc cgcgcctaat ggctgccata aatactcatt
    12301 ggcattttct gcgattgttt ttttagttgc atttttaata atatagccta atatctgtgt
    12361 gcagcagctt tgatattcga ataactttcc ttgttgctcg tccttgcgaa tttttaattt
    12421 tactaataat gcattcaagt catcaccata atacgactca gctgtcaagg aaaaaatatt
    12481 ccaataggac tcgtgccaat cgatatctgc actcatcgta atcacatctt taattgtcaa
    12541 ttttcctttt ggtatctgtg tcaattcagg cacataatct ttcacatcat cattaataga
    12601 ttttatcttt ccaagctcaa tcaatttacc tatcagcaat gcgttgactg tttttgccat
    12661 agaaaatgag cctgaaattg tttcttttgt atatgcttgt gcatagcgct catatacaat
    12721 tttgccatct tgaataacta agtaagcaca cgtttgtcgc tcatcaaaaa aattcaataa
    12781 ttcggcagaa ggtgcctttt tattatagtt ttcactcaat tgccatgcaa ttggtgtgct
    12841 tgatttgctt aatttatgac taggaaaaag cttataatcg tccacattgg cgaaggtgtg
    12901 tttcaagccc atttgtacat ggtatggcaa cacaaaatat attgcggcaa gcactactag
    12961 cgcaatgaat agttttttta ctttttgcat cttctaaaat aaaaaaaatc ggataagtaa
    13021 aataacttat ccgataaaaa ttacaaatga ataaaaaata ctattgaatt actttgactt
    13081 tcactttata gaaataagat tttgcgccac catccatttt gatgtttcca gaataataac
    13141 cctcttcatc aatatcaagc ccttcagtga ctgttccttt tttacgtaaa taatcttctt
    13201 tattgatgta gtagctatca tctggcgaca gagacataat cattactttc gttccttttg
    13261 atatttttgt tgcacttgaa ctcgatttta catcagcagc agctattttg cttgatgtat
    13321 tggagctgct cactttttta agtttaacta aatagaagta aacatcatct ttatcgtttg
    13381 ataatttaac cgtgccacta tatcctcctt catcactttg aaaatcaata tcatcacctg
    13441 caatgccttg ttttcctata taatcggctc tgtcatcata aaatccatct tcaggactaa
    13501 tcgctgtaat ttctactttg tcgccttctt taatatcatc atcattgcgt gcatcgttcc
    13561 aactttcatc agttacttta tcgtcgcctc ctaaccaatc atatacttca ctcgacgcat
    13621 ttgtatcatc gacgtcatca gcagatttct ctacagctgg cacaggatct ttcgaaaatt
    13681 tagctttata aaaatacacc tctttaccgt catcatacgc gaaatctcct gcataatacc
    13741 catcctcttt catctccata tcattttcag taacttttcc ttttttacct atgtgctcat
    13801 gtctatcaga ataataggaa tcttctttgc taatatcagc aacataaaca atagtacctt
    13861 ttttaattgt gcctgtaata aatttgattg atgatgtttt atctagcaat gctgtttttg
    13921 atgatgctac ctgagtcagc tttacttttg agaagtaaca aactctacca gagctagttt
    13981 ctagtgttcc tgaataaaat cctttactgt ctttagtaag atcacttttt acagtagcat
    14041 catttccaac aaaatctgat cgttcgctat aataagcgtc agatttatca atttctaaga
    14101 ttttaacttt actgcctttg ctgaaggtag cttcttgtgc aatagaaaat attgatgaag
    14161 taataaataa acaaaacatc aaaatagaac aaacatttct cataaatgat acttgatttt
    14221 tattaaatgt aataattaaa aaaacaatag tgcaatttat gtaccaaaat aaagcaaaca
    14281 cataccgatt tagtggtatt taaagcaaac ctcttccggt tttgttgagc gtgttgtttg
    14341 attttaggtc tttcattatc gaatctaaaa taccattaat aaaatctttg ctctttggcg
    14401 tgctgtattc tttagcgata tctaaatatt cgttgatggt aacttttact ggaatgcttg
    14461 ggaaatctaa gaattcagca agtgccattc tgattaaaat tacatctaac atggcaagtc
    14521 tatcactttc ccaatttttc aatttcggct tgatgatgac ttggtggtct tttttatttt
    14581 gaatcgtaat tctaaataag tcaatgccaa attgtttgag ttctctaaat ttctctttaa
    14641 gatttccttt ttctaaatga agtttcaatt catttttact cttttgaatc acttcatgca
    14701 ctgcatctac tacatattct gcatcgtctg cccaattggt aaataactct tcaatgatgg
    14761 tattaaaatt ctcgtcttcc aacaagactg catcgagtag aaattttacg atggctctgt
    14821 cggcatctac attaaattct gttccattct cgagataagc tttgtattca ggtgtggcaa
    14881 taaatatttt ataccattgt cttatcgtgt cttcatctaa taattgtgct gttttagctt
    14941 ggctaatttc tgccaaaaat gctttgtcat tattcaaata ttgaatgatg acattgctca
    15001 ataatttagt attgaaattc ttgtcgctat cttgtttaat aaatttacca gctttgattt
    15061 tggcatctac ttccacgaaa ttagacactt cgcgaatgca tagtaataaa tataaatatt
    15121 gatcttttac ggacaagatg cttttttcca gatattgttc aaactgtgag gaggctgcgg
    15181 aagcagtact ttcataagca taaacgtact gcatcgtttt aattctgaca cttcttctgg
    15241 taatcataat gtgataatga actttaggcc acaaagatag tttaaatgta ggaaatacat
    15301 aaagcaaatt tgaccttaga tgttaattaa cgattaattt gaagcaaata aaattattgt
    15361 tattactttc gtcgccatac tttgtcttga tttatacaaa tataaaaagt gaaagaactt
    15421 tacgcagtaa ataaatactt tattaaatac aaatggctct tgttgcttgg tgttttattt
    15481 gttattagtt caaatatatt tggtctttat acaccatcat atattcgata tgctgttgat
    15541 atagtttttg aaaatattgc actctacaaa ctcactgatg gctttgccaa acaagctata
    15601 ctaaagactt attttaatta tttcatcatg tattttgcat gtattatttt aatcacttca
    15661 ataattaaag gcttgttgat gttttttatg cgacaaacgc tcattgttat gtcgcgcaat
    15721 gtagaatacg accaaaaaaa tgagctttat caaaaatatc aaaaattaaa tacaacattt
    15781 tataagcgca ataacacagg cgatttaatg agtagaatca gcgaagacgt atcgagagtg
    15841 cgtatgtata ttggaccagc aattatgtat tttatcaata caggcgcttt gtttttgatg
    15901 gtaatatata tgatgtttaa catcaatgct tatctcagtt tcctggtact attgccattg
    15961 ccaatcttag tatattccat ttacaaagtg agtgatatta ttaataaaaa aagtgagcaa
    16021 atttcagtgg ctttatctgg tttgacctcg caagcacaag aagtctattc aggcattcgc
    16081 attatacaat cgtttggcat acatcagcaa gtgcaacaag attttcatga tgcaagcagt
    16141 gcctatcgtc aaaaaaatat ttccttagca aaagtagatt ccttttttgc gccattaatg
    16201 ttattgctca ttggtttgag cactttactt gttgtttata ttggtggcaa agaagtggta
    16261 aatggaaact ttacagcagg caacattgcc gaatttattt tttatatcaa tatgcttaca
    16321 tggccagtag cctcattagg ttggtgcgta tctttggtac agagagcaga agcctctcaa
    16381 aaaagaatta atcaattttt aaaagatgat aatgaaatcg tcaatacagc gcaatatcca
    16441 ataaaaagca tagactccat aaaatttaac aacgctactt tcatctatcc agacacaggc
    16501 attcaagcaa tttctgattt aagttttact ataaacaaag gcgaaaaggt ggctatcatt
    16561 ggcaaaacag gaagtggaaa atctacgatt gctgaattat tacttcggaa ttatgatata
    16621 gaaagtggca gcatatctat caatcaacac aatatcaaac aaattgacct acatgaatat
    16681 cgaggtaaaa ttggctacac accgcaagat gtttttctat tttctgatac tataaaaaac
    16741 aatattgtat ttgctaatca aattgctgaa aatgaagaaa ttttattaga aaaggccatt
    16801 cagtttgcta aagttacaca cgtacaccat gacattatgc tattaaagca acaatacaat
    16861 acgattgttg gtgagcgtgg cgtcatgctt tctggcggac aaaagcaacg tatttccata
    16921 gcacgctctt tgttcaacaa tcctgaattt gtattgttag acgattgtct ttctgctgtt
    16981 gatgctaaaa cagaaaaaac aatccttcat aatttgcaag caagcttaaa agacaaaacc
    17041 gtactgttta taacacaccg tgtcagtgca attatgcatt ttgacaaaat attagtaatt
    17101 gatgaaggaa aattagtgga acaaggacgg cacgaagagt tattgcgaaa taaaagtatc
    17161 tactatgata tatatcaatt acagcgacaa aaaaacgacc taaaaaatag caatatgtag
    17221 gttattaaca ttgctaaatc gttgttgaat ttttaatttc catagtagca atttttagaa
    17281 tatcattgta cattaaataa aaatagaaac aagtggagaa cgaaaaaaac tttgacaaaa
    17341 atggtgtgta ctctaaacgt gttagagcag gtaaacgcag aacatatttt tttgatgtgc
    17401 gcaccacacg cggtaatgac tattacttaa cgattaccga aagtaaaaaa cgtgcagacg
    17461 atgatactta tgagcgtcat aaaatattca tctacaaaga agattttatg aagtttgtaa
    17521 atgccatgca agaaacggta gattacatca aaacagactt attgcctgat tacgatttcg
    17581 accaacacaa tcatgattat gatgaagatg gtaactacca aagaaatgaa gacaaaaccg
    17641 agaatgagga tgtaagtgct ccgattaaaa caagcgaaac accatcatca gactttaaag
    17701 cacaacagtc aagtgaagaa gataaatggg attaatcaat tcctcttatt aaaaaagttc
    17761 gcaaatgcga acttttttta tgctattttc tatttggcac ccatttttat aggcacattt
    17821 gcttgcacat ggtaagcacc atcaatcacg gttagagatc gataatcact ctccgcttgc
    17881 acacgcagcc aaccataatg aaaatctgtt gggcttgata tggtaataat aataggcaaa
    17941 tacacatcgc ctactccagc gtatcctgtt ttattactgc cacttctttg tgtgacatac
    18001 acattatccc accatcgctt agagccatta tatatgtcag gaatctcctg ctcatacaga
    18061 ttttttactt taaagaaaaa agaagaataa acaaatgctg aatcgcagta aacggctgcc
    18121 gattctccaa atattattgt aactgctgta tcagcagcag catttgaaat tgcagcaatt
    18181 ttaaaatcaa tatgtgcatc atcattcaaa tcaaatgaat ctactttagg caagttcgca
    18241 aaaactgcct tcaccgattt gtcaattttt ttatagatga tattgggatc atcgggttca
    18301 ctctttttac aagaattaat taaaatgaca agcattataa aacaaagaag aacgccaaat
    18361 tgacgaaatc gaaatcgttg aaacatcatc taatttttat taaatataat aggttctgaa
    18421 aacaatcgca atacaattct atttatcagc aaaatattga aaaaactaaa aatgaaaaat
    18481 aaaggtaaaa attttatttt tgtaactcat ggaagtctca aaagaagaat taataatcga
    18541 tgagctaaaa atggaaaatc ctgttttcac agatttccgc acatctgaat tagcagactt
    18601 cctcaacgat tcaccagagt acagcaataa aataatcatg gctttagaac cacatcgtgc
    18661 cgtaaaaacg atgaagttct tagatattaa attgcaaaaa aaactcattc ataccctacc
    18721 ttcaaataat gtagccacac tcatccaaaa tatgcacttt gatgatagaa ctgcattatt
    18781 gagtgaactt accagtgagt cagtaaaaaa gttggtttta tttttaccac atgaagaccg
    18841 catagaaacg cttgctcttt taggctatca agaagatagc gtcggaaggc taatgaatcc
    18901 agattacatt gaagtaaaaa gccactacac ggtacaagat gcactcaata aaattaggcg
    18961 attaggcaga accattgaga atataaattt catttacatc attgatgaaa atgagaaact
    19021 gatagacgat atcaacttaa aagagctatt gataacatct ccagacacca aaataagtgc
    19081 cttgatggat acgaaatatc tagccttaca tgtcaacgat gaccaagaaa ctgcggcatc
    19141 agtttttagg aaaaacaatc gtgctgtgct gcccgtaact gacgacaatg gtgtgctgct
    19201 cggcatcgta actatcgatg atattttgcg tgtagaaagt gaagaagcga cagaagatat
    19261 tcaaaaaatt ggtggtactg aggcattaga cgaaccttac atgaacacac cattttttga
    19321 tttaatcaag aagcgtgcgc catggctggt gatattattt attggcgaaa tgctgaccgc
    19381 aagcgctatg gcatttttcg aagatgagat tgccaaagct attgtcttag tgctttttat
    19441 tccattaatt atttcaagtg gtggaaattc agggtcacaa gcgtcaactc ttatcatccg
    19501 tgcaatggca ttaggcgaga ttacatttga agattggtgg cgcgtcatgc gtcgcgaaat
    19561 aatgagtgga attgtgctag gtagcatttt aggttttatc ggatttttgc gaataataat
    19621 atggaatgcc gtcaatccaa tttatggaga gcattatatc ttagtaggta tcactgttgg
    19681 cgtaacgctt atatttattg tgctttgggg cacattatgt ggcgccatgc ttccaatcgt
    19741 tatcaaaaaa ttaggatttg acccagcaac ctcttccgcg ccattcgtag ctaccttagt
    19801 agatgtaacc ggattagtta tctattttat agtagcttca ttattgctaa gcggctcgct
    19861 attatagctt gttcaaagtt tcctgcataa tagataatgg cattaaattt cctcataata
    19921 cttatctgtt atttatttag taatattcaa aattgtatct ttgcacttca aaaatatttt
    19981 atactaaata attcaagaat atgggtttac agtgtggcat cgtaggatta ccgaatgtag
    20041 gaaaatcaac attatttaat gctgtttcga acaatgcaaa agcacaagcg agcaattatc
    20101 gtttttgcac gattgagcca aatgtaggct tggtagatgt accagatgaa cgtttgcaaa
    20161 aattagcaga aattgttgtt cccaatagaa ccgtttcgac gactattgaa tttgtagata
    20221 tcgctggttt ggttagaggc gcatcaaaag gcgaaggttt aggtaacaaa tttttgggaa
    20281 atattcgcga agtagatgcc atcatccatg taattagatg ctttgaagac gataatattt
    20341 tgcgggaaga aggcgcaatt aatccaattg gcgacaaaga tatcatcgat acggaattac
    20401 aattaaaaga cttagaaagc gtagagaaga agattgccaa aatacagaaa caagctaaag
    20461 taggtggcga tgcaaaggca aaacatgaat atgatgtatt gttacaatgc caacaatgct
    20521 taaacgatgg caaaaacatt cgctctttac aattttcaaa agacgacaga gccttatttg
    20581 ccgatttatg tttcttaaca gacaaaccag tattatatgt ggccaatgtt gatgaggcat
    20641 ctattttaac tggcaataaa tattctgaag cattaacaca aatggctaaa gaagaaaatg
    20701 ctttagttat cgtattaaac aattctgtag aagcacaaat ttcagaaatg gaagaagatg
    20761 acaaagcatt attccaatca gaatatggat tgacagagcc tggcttgaat agattaattc
    20821 gctctgccta cgatttattg caactacaaa cctattttac ggctggcgtg caggaagtta
    20881 gagcttggac tatacataaa ggctggaaag cgccacaagc agcaagcgtc attcacaccg
    20941 attttgagaa aggctttata aaagcagaag tgattgctta tgaagacttt atacattata
    21001 aatcagaagc agcatgccga gaaaatggaa aattgcgtat tgaaggtaaa gaatatattg
    21061 tcaaagatgg tgatgtcatg catttccgtt ttaacgtgta ataaaataag gatagaaata
    21121 aaaaaaatac tatctttgcc actcataaat tattaatttt aaacgcataa actaaaaaca
    21181 tgggacgcgg tgataaaaaa acaagaaaag gtaaaatttt tgcaggttct tttggtaaat
    21241 caagaccaca taaagtaaaa gcaaaagcaa cgcctaaaaa agaagtaaca gaaacaaaag
    21301 caaaaaaagg cgcacctaag aaaacgaaag ctgccgctga gtaattgttt tatcaactta
    21361 taaaaaaagg tcgcacttcg tgcgaccttt ttttatac
//
LOCUS       DAOQQV010000034        21159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_742_length_21159_cov_6.972659, whole genome shotgun sequence.
ACCESSION   DAOQQV010000034 DAOQQV010000000
VERSION     DAOQQV010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21159
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_742_length_21159_cov_6.972659"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PLL59_05340"
     CDS             <1..1063
                     /locus_tag="PLL59_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMY23264.1"
                     /translation="TYIIAPWNYPFQLALSPVVGAVAAGNTCLIRPSRMSENTAKILE
                     KLINENFDPGFLKVVLCDTVQSNQLLEHKFDYIFFTGSPDVGRQIYQAAAKHLTPVTL
                     ELGGKSPCFVDETFNTNWGIKRIVWGKCTNAGQTCVAPDYVLVDKKVKQKFIELFAQT
                     VKEFYGENPQASPDFGRIINEKHFLRLSRFIDKGNVVVGGQTDIANLYIAPTLLDGVS
                     VDDEVMNEEIFGPILPIIEYEKLDDAIKFVNSRNKPLALYIYSDDKMYQERILNETSS
                     GNASINECLMHVGQFELPFGGVGESGIGAYHGKLSFDTFSHLKGILKKSTLSDLKQRF
                     PPYTTSGTNIIRKLIGWFM"
     gene            complement(1100..1173)
                     /locus_tag="PLL59_05345"
     tRNA            complement(1100..1173)
                     /locus_tag="PLL59_05345"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1137..1139),aa:Ala,seq:ggc)
     gene            1266..2360
                     /locus_tag="PLL59_05350"
     CDS             1266..2360
                     /locus_tag="PLL59_05350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMY23265.1"
                     /translation="MSFWEKQLLDEYDFLVVGAGIMGCSVAFELREKYANAKIGIIER
                     DYFSCGASTKNAGFACFGSPTEILHDIQLMGEQNALELVRKRYEGLQILTNRIADAGR
                     TNLGGYELMLQDTLSKSEIQSLNEILFPIFKQTVFVDASKQIHAFQFSNIAQMIYTAF
                     EAQVDSGKLLTAYWQLLQSKNIRFFNGLTVKKLLRNEIEAESSLNGNITCRANKIFIC
                     TNAFPIDTSFKLPIKPGRGQVLITSPIEGLPFSGSFHFDAGFYYFRNVGNRVLFGGGR
                     NLDFTSEETTDFAPNQLILDDLKLKLKEIILSNFSFSIEQEWQGIMAFSENKLPLIHS
                     MNQYATYLMACNGMGVALCPFVAKEIVDKL"
     gene            complement(2374..3123)
                     /gene="kdsB"
                     /locus_tag="PLL59_05355"
     CDS             complement(2374..3123)
                     /gene="kdsB"
                     /locus_tag="PLL59_05355"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HMY23266.1"
                     /translation="MKILGIIPSRFASSRLPGKPLAVLHGKSMIQRVYEQCCKSLTLH
                     ETVVATDDESIAEHLRSFGGRYIMTDKNHLNGTSRCAEVIQQKKSQDFSAIINIQGDE
                     PLLHPNQIDELATLLSNDGISIATQAKKEHQLELLENPNIVKVIIDEHNNAIDFCRII
                     HNKQQLQNIQKAGFFYKHIGMYGFKSDVLLQLMQLKPSKNEIENHLEQLRWLDNGFNI
                     KIGLTSYESISIDTKEDVDKAILILKKQENS"
     gene            3217..4185
                     /locus_tag="PLL59_05360"
     CDS             3217..4185
                     /locus_tag="PLL59_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMY23267.1"
                     /translation="MFRKIFLLFLMFQMPFSMFGQHTSKPTTKKTAYVMHMVKFGETL
                     TKIAKKYEVAINDILKANPSLTANNLSPEQIIRIPNQQNKKPISDLKSNSKSVSPKVE
                     DKNLTQQTITRFHTVEKGQTLYSISKLYNVKPEDIQKWNQLIDNNVKIGSQLKIFVEI
                     NTSSNKAENSKNEVVIKTNTKANVDTNIPKLPTFISADTLSNTIKKPETQAVVNETQS
                     DLSKLFKEKTSNLSVKSVRGTGAPMTTTLGVMENVYFAMHKNLPIGTIVKIKNLTNNK
                     VLYAKVIGKLPDTDENKHVILRYSLGVKKGLLLQNGKCYIQIEYPN"
     gene            4277..5524
                     /gene="hemA"
                     /locus_tag="PLL59_05365"
     CDS             4277..5524
                     /gene="hemA"
                     /locus_tag="PLL59_05365"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HMY23268.1"
                     /translation="MQRYKVVTITHKTSKVNRLKDYLLTDGESSDFPVNSLNAMKEQF
                     TMDELLYLNTCNRVTLFFTTDKKIDQKFLRELFLFINPHFTKEMISLHVSKALVFEGE
                     DAVKHFMGVAASFDSLVIGEREILGQIKTAYLNAKSHGLCGDEIRLAYQQAIVFAKKI
                     HCETRIGEKPVSVVSLAFRTLLERNFFKYANLLIIGAGQTNNLMANLLTKNGAQNVVV
                     YNRTLNKAEELAARFENGKAFALDKLIHHKEDVDVILTCTGANYAVLTKEIFDKLVPK
                     NRKVTIVDLAVPQDVEEEIIALKNVDYIDVASLENKAKENMNFRKSELYKAEAMLEEF
                     ILNFRELHKERRLELALSDIPFQVKDIKDRAFNETFRKDIESLDVHSKEILEKVMQYV
                     EEKYIALPFKASKKSLLNKKFRP"
     gene            5576..>6822
                     /gene="hemC"
                     /locus_tag="PLL59_05370"
     CDS             5576..>6822
                     /gene="hemC"
                     /locus_tag="PLL59_05370"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HMY23269.1"
                     /translation="MKGKSTPIIIGTRGSELALWQANFTKSVLEENGLLVEIKIIKTK
                     GDATQQWNLSFDKIEGKGFFTKELEDALLAKEIDLAVHSCKDLPTAFPDGLTVAAYSK
                     RANPFDKLIIRKECVDERKLLNFKDKAVVGTSSARRKAQLLALRPDVEIHDLRGNVPT
                     RVNYLREGKYDAIILAAAGLERLNIDLSEFVSIPLQSPQFIPAPAQGVLAFQIREDDT
                     VLFDICQLLKDKESTVIASIERQVLHDFHGGCQIPLGVFAQQNEQNIDVWISHATAWN
                     EIPKRMHTIFNGDTIAQQSANVVRQLSNFKAKSVFISSDLSDDDYIIRVLKSHHIPVS
                     FQSFIEFNAIAFTMPTQADWLFFSSKNGVNFFYNNVSMAQIENCKIAAINQGTASAIH
                     QLGYKTDFIGKGNDLLHIAKEFDA"
     assembly_gap    6823..6922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6923..7314
                     /locus_tag="PLL59_05375"
     CDS             <6923..7314
                     /locus_tag="PLL59_05375"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HMY23270.1"
                     /translation="DFIGKGNDLLHIAKEFDALAQGKIVFPQAKTSMQSIQKNMSSNK
                     ISETLVVYENLAASSIAERHEEILVFTSPLSVEAYLSSFSLHSNQTIISIGNTTTKRL
                     NSFGVTEVKTAFEPSYLSVLDEIFAVE"
     gene            7393..>10214
                     /locus_tag="PLL59_05380"
     CDS             7393..>10214
                     /locus_tag="PLL59_05380"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMY23271.1"
                     /translation="MKNFIFLSLFLFIYAIGISQPANDACENAILISNVNNFCSANGA
                     GATNTATDDVATTGGYAASSCWGSGAVNDVWYKFVAIATDVTIVINGNQGSPVGGTLI
                     RPQVTLYSGSCGGTITELNCASPPAGQHIIQLYRGGLTIGATYLVRIDGFNAKKGTFQ
                     YCINNFNPVPAPTSDCPSAVVLCDKSSFTVPSVTGAGLNNTEMNDAACFGGGTVESNS
                     SWYVFTFATAGTFNFTLVPSNPGDDLDFVVYKLPNGVGNCSSKSVQRCMASAPLCTPG
                     GANANTASTGLSPTALYIEEYSGCNSDFVYGACGIGANRCQSQFAKELTVAAGETYAI
                     AINNFTSTGNGFNVTFGGSAQIQGPVSAINDNDADDLLCIGDAITFSDASSPPPTGSL
                     VSWVWNFGVGATPATFNGKTPPAISYSSNGIKTISLTVTSDRGCFITSTKNITVASTP
                     PTVSISASQNPICTGGNVTFMANTTSAGASPTYEWFLNGNPVGTNSNSYTNNSLNNND
                     QIRVRITSSAACNLGAQATSNIITMSIGSAPTVNVNSPSICSGQTASLLASGATSYSW
                     SGGLSAISNPTTPALTTTTTYTVTGTKNGCTGTAVATVTVKPKPTVNVNSPSVCSGQT
                     ASLLASGATSYSWSGGLSSVSNPTTPALTTTTTYTVTGTTNACTGTAVATVTIKPKPT
                     VNVNSPSICSGQTASLVASGATSYSWTGGLSSVNNPTTPALTTTTTYTVTGTTNACTS
                     TAVATITVKPNPSVNVNSPSICSGQTASLLASGATSYSWTGGLPAVSNPITPALTTTT
                     TYTVTGTTNGCTGTAVATVTVKPNPTVNVNSPSVCSGQTASLLASGATSYSWTGGLSA
                     ISNPTTPTLTTTITYTVTGTTNGCTGTAVATVTVKPNPTVNVNSPSICSGQTASLLAS
                     GATSYSWSGGLSSVSNPTTPALTTTTT"
     assembly_gap    10215..10314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10315..11834
                     /locus_tag="PLL59_05385"
     CDS             <10315..11834
                     /locus_tag="PLL59_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY23272.1"
                     /translation="GLSSVSNPTTPALTTTTTYTVIGITNACTGTAIATITVNQRPTV
                     SLNSPIICSGQTASLLASGATSYSWSGGLAAVNNPITPILTRNTSYTVTGSINGCTGT
                     AVSTMVTVKPTKSTSIDSVICFGESVTIGSQTFTTSGNYSVVLQTSENCDSIIHLKLT
                     ANNSYTPTVSIVANKNDICNRELVLFTATATNTVVTPTYQWQLNGVNVGANSNRYSNS
                     TLNNGDIIKVIITSSAPCVNPSTLSSNEIVMKVNTIAYTKPHIEYCQKDSQLVNLQIN
                     TIPSTPYSIVLSDGTIINGSTMSVNTTSSGNIPITITYGNNCSVRDVLPVVVNSLPNI
                     NAIVDTPHTRYQHIVQLDVLSNNAIQSYHWLASNLVNNDTIKNPTSIIKASSLFIVEV
                     KDNKNCKRTDSVMVEMIDECDDDYIFMPSAFSPNNDGVNDCFGILLPPILTDYKLIIF
                     NRWSEKIFETSDKDICWDGTYKGEAVQSDSYVYLLSFTCYNEERLSKKGTVSVLR"
     gene            complement(11839..12456)
                     /locus_tag="PLL59_05390"
     CDS             complement(11839..12456)
                     /locus_tag="PLL59_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HMY23273.1"
                     /translation="MLLSISLLSWKTWTVPLIGAFIGWITNWLAIKMLFHPRRPVKVL
                     FLTFHGIFPKNKPRIAEKLGTVVQRDLINFSDIKDRLQDPDALNNFKEEIATRVDDAL
                     RDRIEKSTLASILVPEPLIQSVHKTIVDEIEANLPTVIGSSIDKIEDKLDIQQLVRNK
                     VNNFSDEKLEQLLLDITAKEFTFIEIIGGVLGFVIGIIQLLISVL"
     gene            complement(12481..13128)
                     /gene="pyrE"
                     /locus_tag="PLL59_05395"
     CDS             complement(12481..13128)
                     /gene="pyrE"
                     /locus_tag="PLL59_05395"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMY23274.1"
                     /translation="MENNAYKEKLAEYLLQIKAIQLNTQQPFTWTSGIKSPVYCDNRK
                     LLSYPEVRTYVKNGLISLLKEYFPQTECIAGVATAGIPHGAIIADALDLPFVYVRSKP
                     KEHGLTNTIEGDLKHGQRVLVVEDTISTGGSSLKAVEDLRAVGAEVSGMIAIYKYGFE
                     SAKQKFDDAGVVLKTLTSYDFLIRVAIENGYVSAEELETLNSWRQNPSAWLTVQS"
     gene            13191..13826
                     /locus_tag="PLL59_05400"
     CDS             13191..13826
                     /locus_tag="PLL59_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMY23275.1"
                     /translation="MYKLFINNKVVFLCQNPAFVENLMNDNFIVLQYKNKTDFEAILQ
                     MILGDANQSNLVLFSKDVDAMFSDVCSFFECIEAAGGVVSNTDGEILLIYRRGFWDLP
                     KGKIEQNETIEHAAIREVEEETGIGKLNIIQPIHFKNLKNKATYHSYLIKNKPALKVS
                     YWFDMQTTFSGKLVPQTEEDIDQVIWVSPNELPNYFDTMYPSIVDVLKEII"
     gene            complement(13827..14738)
                     /locus_tag="PLL59_05405"
     CDS             complement(13827..14738)
                     /locus_tag="PLL59_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HMY23276.1"
                     /translation="MEILQQPIFLILLFIVGFILQFISIIPATLHKKINQFILWIPLP
                     AIVLAKIPFLEISKQVILPIGAAWITFIGAILFAIIIAKFFMFDKKTIACIILCCGLG
                     NTSFVGYPFLQYFYGEESLKYAIFVDQPGSFLILSTCGVFVATYFSASTISIKTITER
                     LLKFPPFFAFIVALFLPTSLIEGMPSILLNNIGKLMVPLALLSLGLQFKISAKTLPWK
                     KLGIGLLYKLMIAPFIVYIMSIIIIKKHDIISTVSIIECAMPPMITASILAVEYDLDS
                     ELASTLPTIGILISIPTLFFWKWLIGY"
     gene            complement(14740..15705)
                     /gene="ftcD"
                     /locus_tag="PLL59_05410"
     CDS             complement(14740..15705)
                     /gene="ftcD"
                     /locus_tag="PLL59_05410"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HMY23277.1"
                     /translation="MFTTSDQLIECVPNFSEGRNKASIEAIANAIRGVAFVKLLHTDI
                     GVDANRTVFTFIGKPDAVIEAAYRAIKVAEKIIDMRAHKGEHPRMGACDVCPLIPFQN
                     ISLVEVIELSKKLGKRLGDDGIPVFLYEKSANTALRQNLSYIRKNEYEGIAEKIKKAE
                     WKPDFGPQIFHPTFGIMALGARNFLIAYNINLQSKDLTLAKKIAKEIRDIRTKNDGSK
                     LSNLFQHVKAIGWWMQAYNCTQISTNITDIHQSPIIDVFTAIKNICTAHHVETNGSEL
                     IGLIPKQAFAHQSLSIDEAINYLGLNAVKPFTKEKNIIEYCLNLT"
     gene            16022..16591
                     /locus_tag="PLL59_05415"
     CDS             16022..16591
                     /locus_tag="PLL59_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY23278.1"
                     /translation="MTSELTFFFQQYVHLPADELDDITSKFTHKIIKKNEFVLQEGEV
                     CKDLIFVQKGCLRLFYMLNDVEVSVWFALKHSSAIEIYSFISETPTQYFLQAIEDTEI
                     LYLPKSALNKLYESHPLMQEMMRKFWEDVILHLLSRFTSLQRDTAEQRYLELLNKPEL
                     LQSIPQKYLASFIGVTPTSLSRIKKNIKK"
     gene            16656..17483
                     /locus_tag="PLL59_05420"
     CDS             16656..17483
                     /locus_tag="PLL59_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMY23279.1"
                     /translation="MLLQIQAASGFNLIEKLQNLAFAFAPKLVGAIIVYIVGVFVIKR
                     LATVLTKIMQRKNYDKSLQTFLVSLIKVMLTILLLLSIAGMIGIDITSFAAILAGAGL
                     AIGAALNGSLGNLAGGVMMLVFKPFKVGDLIEAQGVMGKVIEQGVFATTILTGDNKTI
                     IIPNGPLSTGVITNYNTHGNLRVDLNVAIAMTQDIDVARAVATEAMLSHPRVLKVPAP
                     EVSVLKIADGMCSLAIRPYTVQADYWDVYFGVQELVKKAFDAKGIEGPTPTRIIIQK"
     gene            complement(17561..17995)
                     /locus_tag="PLL59_05425"
     CDS             complement(17561..17995)
                     /locus_tag="PLL59_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMY23280.1"
                     /translation="MAQINPYIIFNGNAEEAFLFYKSVFGGEFAMLIRFKDMPSEHNN
                     QIQEDEANKIMHIALPIGKNVLMANDVPAFMGKVNESENRSKISISAESKEEADRIFN
                     ALSVDGAVEMPIGDSPWGSYFGMFRDKYGIEWMIDFDPRYHI"
     gene            18133..19458
                     /gene="ahcY"
                     /locus_tag="PLL59_05430"
     CDS             18133..19458
                     /gene="ahcY"
                     /locus_tag="PLL59_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HMY23281.1"
                     /translation="MSTTSPSIDFNLKYKVKDMSLAEWGRKEITLAEAEMPGLMALRE
                     EFGPSQPLKGARIAGCLHMTIQTAVLIETLVALGAEVTWSSCNIFSTQDHAAAAIAAA
                     GIPVFAWKGMNEEEFNWCIEQTLFFGSEDRPLNMILDDGGDLTNMVFDNYPELAKGIK
                     GLSEETTTGVHRLYERMENGTLLIPAININDSVTKSKFDNKYGCRESCVDAIRRATDV
                     MIAGKVAVVAGYGDVGKGSAESLRGAGARVIVTEIDPICALQAAMEGYEVKKMIDAVK
                     EADIVVTTTGCRDIITGEHFKVMKDKTIVCNIGHFDIEIDVAWLNQNYGHTKNTIKPQ
                     VDLYTIEGKDVILLAEGRLVNLGCATGHPSFVMSNSFSNQVIAQLELWSNADKYENKV
                     YMLPKHLDEKVARLHLAKIGVELDVLTAEQSSYLNIPAAGPYKPEYYRY"
     gene            19763..19838
                     /locus_tag="PLL59_05435"
     tRNA            19763..19838
                     /locus_tag="PLL59_05435"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19798..19800,aa:His,seq:gtg)
     gene            complement(19847..20425)
                     /locus_tag="PLL59_05440"
     CDS             complement(19847..20425)
                     /locus_tag="PLL59_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMY23282.1"
                     /translation="MDSLLQLDYSIFHFINHTISNSFLDVLLPFLRHANNWIPLYIFL
                     EIYLLYKYNIEALKYILYIVLAFALADYISAGIIKPFVARLRPCHSAYLQANLLLGHC
                     GGKWSFPSTHATNHIAIALSIVMANIFTKRWINLCWIIWAIAIGFAQIYVGVHFPSDV
                     LAGIILGSMIAFINWKYILPAIEKMYERLLIR"
     gene            complement(20425..21072)
                     /locus_tag="PLL59_05445"
     CDS             complement(20425..21072)
                     /locus_tag="PLL59_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMY23283.1"
                     /translation="MSKVIVIIGAGQGIGKAVATQFGKQGFSIALIARKSEKLEILKQ
                     QLIAEGIDAHAFTGDAGDPESMREAFNQIWEIWEHIDTIHYNVAKIKNVNIAIETADG
                     LTRDFKINVASVMTALHLVLPDMEKKEKGTILLTGGGFALEPNPDYGSLAIGKAGIRN
                     LAASLHQALKSKNIFVGTVTVCGLVSPNAEKHTPANIATQFWKLYTERNEFEIQF"
BASE COUNT         7028 a         3473 c         3769 g         6689 t
ORIGIN      
        1 tacttatatt attgcacctt ggaattatcc atttcaattg gcattgtcgc cagtagtagg
       61 tgccgtagct gctggaaata catgcctgat tcgtccatct cgaatgtcgg aaaatacagc
      121 aaagatttta gagaaattga ttaacgaaaa ttttgatcca ggatttttga aagtggtgct
      181 gtgcgataca gtgcaaagca atcaattgtt ggagcataaa tttgattata tcttctttac
      241 aggcagtcca gatgtaggtc gccaaattta tcaagcagcg gcaaaacatc taacgccagt
      301 aacactggaa ttaggtggaa aaagtccatg ttttgttgac gaaaccttta ataccaattg
      361 gggaattaaa cgcatcgttt ggggaaaatg caccaatgcc ggtcaaacat gtgttgcgcc
      421 tgactatgtt ttggtagata aaaaagtaaa acagaaattt attgaattgt ttgcgcaaac
      481 agtgaaagaa ttttacggtg aaaatccgca ggcaagtcct gattttggca gaataataaa
      541 cgaaaaacat ttcttgcgtt taagccgatt tatcgataaa ggaaatgtag ttgtaggtgg
      601 tcaaaccgat atcgctaatc tttatattgc accaactttg ttagatggtg tttctgtaga
      661 tgatgaagtg atgaatgaag agatattcgg tccaattttg ccaattattg aatacgagaa
      721 attagatgat gcgatcaaat ttgtcaattc aagaaacaaa ccattggcat tgtatattta
      781 ctcagatgat aaaatgtatc aagagcgtat cttgaatgaa acatcttctg gaaatgcgtc
      841 tatcaatgaa tgtttgatgc atgtaggtca atttgaattg ccgtttggtg gtgttggcga
      901 aagtggcatc ggtgcttatc atggcaaatt gagttttgat acttttagtc atttaaaagg
      961 cattcttaaa aaatcaacat tgagcgattt aaaacaacgt tttccgcctt atacaacaag
     1021 cggtacaaat attatacgaa agttaatcgg ctggtttatg taatagaaaa tcctgagtgc
     1081 aaactcagga ttttttttgt ggagaatatc ggattcgaac cgatcgcctc ttcactgcca
     1141 gcgaagcgct ctagccaaat gagctaattc cccaatttga tgcgatgcaa aagtaaacaa
     1201 ttattttatt tttcatatca ttcgcatttt ctattcctaa gctgtaattt tataaataga
     1261 tgatgatgtc attttgggaa aaacaattgc tcgacgaata cgactttttg gtggtaggcg
     1321 ctggaataat gggctgctct gtggcatttg aattacgaga aaaatatgca aatgcaaaaa
     1381 ttggaattat agaacgcgac tacttttcct gtggtgcttc tactaaaaat gcgggctttg
     1441 cctgttttgg ctcaccgacg gaaatcctgc acgacataca attaatgggc gaacaaaatg
     1501 ctttagaatt agttcgcaaa cgatacgaag gcttgcaaat attgacaaac agaattgcag
     1561 atgcaggaag aacaaatctt ggtggatatg aattgatgtt acaggataca ttatccaaaa
     1621 gtgaaataca aagcttgaat gaaatactat ttccaatttt caaacaaaca gttttcgtag
     1681 atgcgtccaa gcaaatacac gcgtttcaat ttagcaatat tgcacaaatg atttacaccg
     1741 ctttcgaggc acaagttgat tctggaaaat tacttaccgc atattggcaa ttgctacagt
     1801 cgaaaaatat tcgctttttt aatggcctta ctgtcaagaa gcttttgaga aacgaaatag
     1861 aagcggagag tagtttaaat ggaaatatta catgtagagc caacaaaatt tttatttgta
     1921 caaatgcttt tccgatagac acatctttta aattgcctat taagccagga agaggacagg
     1981 tgttaattac atcaccaata gaaggtttgc cttttagtgg tagttttcat tttgatgctg
     2041 gattttatta ttttagaaat gtaggcaata gagtgctatt tggcggtggc cgaaatcttg
     2101 attttacaag tgaagaaaca acagacttcg caccgaatca attgatttta gatgatttga
     2161 aattaaaatt aaaggaaatt atcttatcga atttttcatt ttccatagaa caagaatggc
     2221 aaggcataat ggcattttca gaaaataaat taccacttat ccactcaatg aatcaatatg
     2281 ctacttattt gatggcatgc aatggaatgg gtgtagcctt gtgtccattt gttgccaagg
     2341 aaattgttga caaattgtaa atttctcata caattaagag ttttcttgtt tctttagaat
     2401 aagaattgct ttatctacat cttctttggt gtcaatgctg atgctttcat aactggtcaa
     2461 tcctattttg atgttaaatc cattgtctaa ccagcgcaat tgttcaaggt ggttttctat
     2521 ttcatttttg cttggtttta attgcattaa ttgtagtaaa acatctgatt taaaaccata
     2581 catgccaata tgtttataaa aaaaaccagc tttttgaata ttctgaagct gttgtttgtt
     2641 atgaataatt ctgcaaaaat caattgcatt gttgtgttca tcaataatga ctttgacgat
     2701 gtttggattt tctaataatt cgagctggtg ttcttttttt gcttgtgtgg caatcgaaat
     2761 accatcattt gatagtaatg tggcaagctc atcaatttga tttggatgca acaaaggttc
     2821 gtctccttga atattgatta tagcagaaaa atcttgagat tttttttgtt gtataacttc
     2881 tgcacatctc gaagtgccat ttaagtggtt tttatcggtc attatatatc ttccgccaaa
     2941 gcttcttagg tgttcagcaa tactttcatc atccgttgca acgaccgttt catgaagtgt
     3001 caaagatttg caacactgtt catatacgcg ctgtatcatt gatttgccat gcaggacagc
     3061 caatggtttg ccaggtaatc tagaagaagc aaaacgagac ggtatgatac caagaatttt
     3121 caattttttt ctatttggca caaatgtact gtaaactatt gttgaaaaat agtgtctttt
     3181 tctaactttt ttaagtggat ttttgttgta atgtttatgt ttcgaaaaat ctttttattg
     3241 tttttgatgt tccaaatgcc attttctatg tttggacaac acacatctaa gcctacaacg
     3301 aagaaaacgg cttatgtaat gcacatggtt aaatttggtg aaacactgac taaaatcgcc
     3361 aaaaaatatg aagttgctat taatgatatt ctgaaagcca atccatcttt gacggcaaac
     3421 aatttaagtc ctgagcagat tataagaatt ccgaatcagc aaaataaaaa acctataagt
     3481 gatttaaaat caaacagtaa atcagtatca cctaaagttg aggataaaaa tttgacacag
     3541 cagacaatca ctagatttca tactgttgag aaaggacaaa ccttgtattc tatttccaaa
     3601 ttgtataatg taaaaccaga agatattcaa aaatggaatc aattaattga taataatgta
     3661 aagataggca gtcagttaaa gatatttgtt gaaattaata catcaagtaa taaggcggaa
     3721 aatagtaaga atgaagttgt tattaaaaca aatacaaaag ctaatgtaga tactaatatt
     3781 ccaaaattgc ctacatttat ttctgctgat actttaagta atactattaa aaaaccagaa
     3841 acacaagcag ttgtaaatga aacacaatct gatttatcca aactatttaa agaaaaaact
     3901 tccaatctaa gtgtaaaatc tgttcgagga actggtgctc cgatgacgac cacattaggt
     3961 gttatggaga atgtctattt tgctatgcat aaaaatttac cgataggcac aattgtaaaa
     4021 atcaaaaatt tgacaaacaa caaagtgctg tatgcaaagg ttattggtaa attgccagac
     4081 acagacgaaa ataaacatgt tatattaaga tattcattag gcgtaaaaaa aggattgttg
     4141 ctgcaaaatg gaaaatgcta tatacaaata gaatatccga attaaacgat aaatagtttg
     4201 ataacccaaa tatgatgaaa atcatttttt tataaggtaa attgttatca tatttgcata
     4261 gtaaaaaagc tagaaagtgc aacgttacaa agtagtaacc ataacacata aaacatcaaa
     4321 agtaaatagg ttgaaagatt acctattgac agatggtgag tcatcagatt ttccggtaaa
     4381 tagcttaaat gccatgaagg aacaatttac gatggatgaa ttgttgtact taaatacttg
     4441 taatcgtgtt accctatttt ttactacaga taaaaagata gaccaaaagt ttttgcgaga
     4501 acttttctta tttatcaacc cacacttcac caaagaaatg ataagccttc atgtgtcaaa
     4561 agcattggtt tttgaaggtg aagatgctgt aaagcatttt atgggcgtcg ctgcttcttt
     4621 tgattcattg gtaataggcg aacgggaaat tttaggccaa ataaaaacag cctatctaaa
     4681 tgccaaaagc catggtttgt gtggtgatga aattcgtttg gcatatcaac aagcaatagt
     4741 ttttgctaaa aaaatacatt gtgaaacacg tattggcgaa aaacctgttt ctgtagtgtc
     4801 cttggcattt agaactttac tggaaagaaa tttctttaaa tatgctaatt tattaatcat
     4861 tggagctgga caaaccaata atttaatggc taatttgctt acaaaaaatg gtgcacaaaa
     4921 cgttgttgta tataatcgaa ctttgaacaa agccgaagaa ttagcagctc gatttgaaaa
     4981 tgggaaagca ttcgctttag ataaattgat tcatcacaaa gaagacgtag atgtaattct
     5041 gacttgtact ggtgctaatt atgcggtgtt gacaaaggaa atatttgaca aattagtgcc
     5101 taaaaataga aaagtaacta tcgttgattt agctgtgcca caagatgtag aagaagaaat
     5161 tatcgcacta aaaaatgtcg attatatcga tgttgcttct ttggaaaata aggctaaaga
     5221 aaatatgaat tttagaaaaa gcgagctgta taaagctgaa gccatgctcg aagagtttat
     5281 tctaaatttc agagaattac ataaagaaag aagattggaa ttggctttgt ctgatattcc
     5341 atttcaagta aaagatatta aagacagagc ttttaatgaa acattcagaa aagacatcga
     5401 aagcttagat gtgcattcta aagaaatttt ggaaaaagta atgcaatacg tcgaggaaaa
     5461 atatatcgcg ctacctttta aggcgtcaaa aaaatcattg ctgaataaaa aattcagacc
     5521 ttaatttcag tacatacatt cgttaataag cactacaagc atagtaatat attgcatgaa
     5581 aggaaaatca acacctataa taattggaac acgtggcagc gaattagcat tatggcaggc
     5641 taatttcaca aaatccgttt tagaggaaaa cggcctcttg gtagaaataa aaattattaa
     5701 aaccaaagga gatgccacac aacaatggaa tctaagcttt gataaaatcg aaggaaaagg
     5761 cttttttact aaagaattag aagatgcatt attggcaaaa gaaattgatt tagcggttca
     5821 ttcttgtaag gatttgccaa ctgcatttcc tgatggtttg acagtagctg cttattcaaa
     5881 acgagccaat ccatttgata aactaatcat tcgcaaagaa tgtgtagatg aaagaaaatt
     5941 attgaatttc aaagataaag cagttgttgg tacttcttct gccagacgaa aagcgcagct
     6001 gcttgcctta cgaccagatg ttgaaattca tgacttacgt ggtaatgtgc caacgcgtgt
     6061 taattatttg cgcgaaggaa aatatgatgc aattatttta gcagcagcag gattagaacg
     6121 attaaacatc gatttaagtg agtttgtttc cattccttta caatcacctc aatttattcc
     6181 agctcctgcg caaggtgttt tggcatttca aatccgagaa gatgatactg tattatttga
     6241 catttgccaa ttattgaaag ataaggagag cactgtaata gcttcaattg agcgacaagt
     6301 attgcatgat tttcatggag gctgccaaat tccattaggt gtttttgcgc aacaaaatga
     6361 acaaaatata gatgtttgga tttctcatgc tactgcatgg aatgaaattc caaaacgtat
     6421 gcatactatt tttaatggag atactatcgc acagcaatca gctaatgtgg tacgccagct
     6481 ttctaatttt aaagcaaaat ctgttttcat ttcttctgat ttgagtgatg atgattatat
     6541 aattcgagtc ttgaaatcac atcatattcc agtttctttt caatcattta ttgaatttaa
     6601 tgctattgca tttacaatgc caactcaagc agattggttg ttttttagta gtaagaatgg
     6661 tgttaatttc ttttataata atgtttcgat ggctcaaatt gaaaattgca aaatcgcagc
     6721 aatcaaccaa ggcacagctt ccgcaattca tcaactagga tataaaacag attttattgg
     6781 aaaaggtaat gatttgttgc atatagcaaa agagtttgat gcnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nncagatttt attggaaaag gtaatgattt gttgcatata
     6961 gcaaaagagt ttgatgcttt agcacaaggt aaaattgtat ttccacaagc taaaacgtct
     7021 atgcaaagca tacaaaagaa catgtcatca aataaaattt cagaaacttt agtggtttat
     7081 gaaaatcttg ctgcgtcatc aattgcagaa agacacgaag agattctagt atttacaagt
     7141 ccattgagtg tagaggcata cttgtcttcc ttttcattac attcaaatca gacaattatt
     7201 tctattggaa atacaactac aaaacgatta aattcctttg gtgtaactga ggtcaaaaca
     7261 gcatttgaac ccagttatct atcagtttta gatgagattt ttgcggtgga ataagtaata
     7321 cacacatcac tcatatttta ttcacaaatt ttaacattcc tgcatttttt cgtaaattaa
     7381 actttgctat ttatgaaaaa ttttattttt ttaagcctat ttctttttat atatgcaata
     7441 ggtatatcac agcctgcaaa tgatgcttgt gaaaatgcaa ttttaatttc taatgttaat
     7501 aatttctgtt ctgcgaatgg agctggagct acgaacacag ccacagatga tgttgccaca
     7561 acaggtggtt atgctgcatc gagttgttgg ggaagcggcg ccgttaatga tgtttggtac
     7621 aagttcgttg ccattgcaac agatgtaaca attgtaatta atggcaatca aggttcgcct
     7681 gtaggcggta cattaatcag gccacaagtt accttatatt caggttcatg tggtggaaca
     7741 atcacagaat taaattgtgc ttctccacct gctggtcaac acattattca attatacaga
     7801 ggcggtttaa caataggagc aacttatttg gtgagaattg atggctttaa tgcaaaaaaa
     7861 ggaacttttc aatattgtat caataatttt aatcctgttc cagcgccaac aagcgattgc
     7921 ccaagtgctg tcgtactgtg cgataaaagt tcatttacgg tgccaagtgt tactggtgcc
     7981 ggtttaaata atacagaaat gaatgatgcc gcttgttttg gtggcggcac agtagaatct
     8041 aactctagtt ggtatgtatt tacttttgca actgctggca cttttaattt tacgctagtg
     8101 ccttcaaatc ctggtgatga cttagatttt gtcgtttata aattaccaaa tggtgttgga
     8161 aattgttcaa gtaaaagcgt gcagcgttgt atggcatcag caccattgtg cacaccaggt
     8221 ggcgcaaatg caaataccgc atcgacaggt ttgtctccaa cggctttgta tattgaagaa
     8281 tattcaggat gcaattctga ttttgtttat ggcgcatgcg gtataggcgc aaaccgttgt
     8341 caaagtcagt ttgcaaaaga attgacagtt gccgcaggcg aaacttatgc cattgcaatt
     8401 aataacttta catcaacagg taatggcttt aatgttacat ttggtggttc cgcacaaata
     8461 caagggccag tttctgcaat aaatgataat gatgcagatg atttactttg tatcggtgat
     8521 gcgattactt tctctgatgc ttcatctcca ccaccaacag gctcacttgt gtcgtgggtt
     8581 tggaattttg gcgttggagc aacgccagca acttttaatg gtaaaactcc acctgcaata
     8641 agctattctt cgaatggtat aaaaactatt tcactaaccg tcacaagcga tagaggttgt
     8701 tttatcactt ctactaaaaa tatcacggtt gcttctacac caccaacagt gagtatttct
     8761 gcttcacaaa atccaatttg tacaggtggc aatgttacat ttatggcaaa tacaactagt
     8821 gctggcgcat caccaactta tgaatggttt ttaaatggca atccagtcgg cacgaattcc
     8881 aattcatata ccaataattc actcaataac aatgaccaaa ttagggtaag aataacgagt
     8941 agtgcagctt gtaatttagg tgctcaagcg actagtaata taattactat gtctattggt
     9001 tcggcgccaa cagtaaatgt taatagtcca agtatatgca gtggacaaac cgcgagttta
     9061 ttggcaagtg gtgcaacatc atatagttgg agtggaggct tatcagctat aagcaatcca
     9121 acaacaccag cattaacaac aactacaact tatacggtca caggcacaaa aaatggctgt
     9181 acaggcacag cagtagccac tgttacagta aaaccaaagc caacagtaaa tgtgaatagt
     9241 ccaagtgtct gtagtggaca aactgctagc ttattggcaa gtggtgcaac atcatatagt
     9301 tggagtggag gcttgtcgtc agtaagcaat cctacaacac cagcattaac aacaacaaca
     9361 acttatacgg taacaggaac aacaaatgct tgtacaggca cagcagtagc cacagttaca
     9421 ataaaaccga agccaacagt aaatgtaaat agtccaagta tatgtagtgg acaaactgca
     9481 agcttagtgg cgagtggtgc aacatcatat agttggactg gaggcttgtc atcagtaaac
     9541 aatccgacaa caccagcatt aacgacaact acaacttata cggtaacagg aacaacaaat
     9601 gcttgtacta gtacagcagt agccactatt acagtaaaac caaatccatc agtaaatgta
     9661 aatagtccaa gtatatgtag tggacaaaca gcaagcttat tggcaagcgg tgcaacatca
     9721 tatagttgga ctggaggctt gccagctgtc agcaatccaa taacaccagc attaactaca
     9781 actacaactt atacggtcac cggtacaaca aatggctgta caggcacagc agtagccact
     9841 gttacagtaa aaccaaatcc aacagtaaat gtaaatagtc caagtgtctg tagtggacaa
     9901 actgcaagct tattggcaag tggtgcaaca tcatatagtt ggactggagg cttgtcagct
     9961 ataagcaatc cgacaacacc aacattaaca acaactataa cttatacggt cactggaaca
    10021 acaaatggct gtacaggcac agcagtagcc actgttacag taaaaccaaa tccaacagta
    10081 aatgtaaata gtccaagtat atgcagtggt caaacagcaa gtttattagc aagtggtgca
    10141 acatcatata gttggagtgg aggcttgtca tcagtaagca atccaacaac accagcatta
    10201 acaacaacta caacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaggct
    10321 tgtcatcagt aagcaatcca acaacaccag cattaacaac aactacaacc tatacggtca
    10381 taggaataac aaatgcttgt acaggcacag caatagcaac cataactgta aatcaaagac
    10441 caactgttag tctaaatagt ccaattatat gtagtggaca aactgcgagc ttattagcaa
    10501 gtggtgcaac atcatatagt tggagtggag gcttagcagc tgtcaacaat ccgataacac
    10561 caatattgac tagaaacaca agctatacgg ttacaggctc tatcaatggt tgcacaggaa
    10621 ctgccgtttc gacaatggta acagttaagc caacaaagtc aacaagcatc gatagcgtta
    10681 tttgttttgg agaaagtgtt actattggta gccagacttt tactacaagt ggaaattata
    10741 gtgtagtctt gcaaacttct gaaaattgcg atagcattat ccatttaaaa ttgacggcaa
    10801 ataattcata tacgccaaca gtaagtattg ttgccaataa aaatgatatt tgcaatagag
    10861 aacttgtttt gtttacagct actgcaacaa atacagttgt tacgccaaca tatcagtggc
    10921 aattaaatgg tgtaaatgta ggtgctaata gtaatcgtta cagtaattct acgttgaata
    10981 atggagatat tattaaagtg attattacat catcagcgcc ttgtgttaat ccttcaactt
    11041 tatcttcaaa tgagattgtg atgaaagtaa atacaattgc ttacacaaaa cctcacattg
    11101 aatattgcca aaaagattca caacttgtaa acctacaaat taatacgata ccatcaacac
    11161 catatagcat tgtgttaagc gatggaacaa ttattaatgg cagcacaatg tctgttaata
    11221 ctacaagcag tggcaatatt ccaattacaa ttacatatgg aaataattgc agtgttcgtg
    11281 atgtgttacc tgttgtcgtc aattctctgc caaatataaa tgcgattgtc gatacacctc
    11341 atacaagata tcagcatata gtacaattag atgtgctttc gaacaatgct attcaaagtt
    11401 atcattggtt agcatcaaac ttagtaaaca atgatactat aaaaaatccg actagtatta
    11461 ttaaagcaag cagcctattt atcgtagaag ttaaagacaa taagaattgc aaaaggacag
    11521 atagtgtaat ggtagaaatg attgacgaat gtgatgatga ttatattttt atgcctagtg
    11581 cattttcgcc aaataatgat ggcgttaatg attgctttgg aattttgttg ccacctatct
    11641 taacagatta taaattaatt atttttaatc gatggagtga gaagattttt gaaacttcgg
    11701 ataaagatat ttgctgggac ggcacgtata aaggtgaagc agtgcagtcg gatagttatg
    11761 tttatttgct aagttttaca tgttataatg aagaaagatt aagcaagaaa ggaacggttt
    11821 ccgttttgcg ataaggtgct ataacacact aataagaagt tgaataattc caattacaaa
    11881 acctaaaacg ccaccaatta tttctataaa cgtaaattct tttgcagtaa tatctaataa
    11941 taactgttcg agtttttcat cagagaaatt gtttacttta tttcgaacta attgttgaat
    12001 atctagcttg tcttctattt tatcaattga agatccaata acagtaggca agttggcttc
    12061 tatctcatcg acaattgttt tgtgcacact ttgaattaaa ggctctggaa ctaatatact
    12121 tgcgagcgtg cttttttcaa ttctatcacg caaagcatca tcaacgcgag ttgcaatttc
    12181 ttctttaaaa ttattcaaag catctggatc ttgcaatcgg tctttaatgt ctgaaaaatt
    12241 aattaaatca cgctgcacaa cggtacctaa tttctccgcg attcgtggtt tgtttttcgg
    12301 aaatatgccg tgaaatgtca gaaataaaac tttaactggc cttcttggat gaaataacat
    12361 tttaatagca agccaattag taatccaacc tataaaggca ccaatcagcg gcaccgtcca
    12421 tgttttccaa ctcagtaaac taatagataa aagcatataa ttttttttgc aaatgcttag
    12481 ttatgattgt acagttaacc atgctgaagg attttgcctc caactattca atgtttccaa
    12541 ctcttctgca gaaacatatc cattttctat ggccacacga atcaaaaaat cataactcgt
    12601 taatgtcttt aacacaacac cagcatcatc aaatttttgc tttgcacttt caaaaccata
    12661 tttataaata gcaatcatgc cacttacttc tgcacctaca gcacgtaaat cttcaactgc
    12721 tttaagtgag ctgcctccag ttgaaatagt atcttctact acaagaacac gttgtccatg
    12781 ttttaaatcg ccttcgattg tatttgtcag accatgctct ttcggttttg aacgcacata
    12841 aacaaagggc aagtctaatg catcagcaat gatagcacca tgcggaatgc cagcagttgc
    12901 tacgcctgca atgcattctg tttgtggaaa atattctttt aacaaactga ttaaaccatt
    12961 ctttacgtac gttcttactt ctggatacga taataatttc cgattatcac aatatactgg
    13021 agattttata ccactcgtcc atgtaaatgg ttgctgtgtg tttaattgta tggcttttat
    13081 ttgtaataaa tattcggcca atttttcttt atatgcatta ttttccattt cttagcaatt
    13141 aaaattgtaa aacataattt agtacttatt aaaatacttt aactgcataa atgtataaac
    13201 tttttatcaa taacaaggta gttttcttgt gtcaaaatcc ggcatttgta gaaaacttaa
    13261 tgaatgataa ttttattgtc ttacaatata aaaataaaac tgattttgaa gccattttgc
    13321 aaatgatatt aggtgatgca aatcagagta atttagtact cttttcaaaa gatgtagatg
    13381 ctatgttttc cgatgtttgc tcgtttttcg agtgtataga agccgctggt ggtgttgtta
    13441 gcaatacaga tggagaaata ttattaatat atcgccgtgg tttttgggat ttgccaaaag
    13501 gaaaaattga gcaaaatgaa accattgaac atgccgcaat ccgcgaagtt gaagaggaaa
    13561 cgggcattgg gaaattaaac attattcaac caatacattt taaaaatcta aaaaacaaag
    13621 ccacctatca ttcctattta ataaaaaaca aaccagcgct aaaagtttcc tattggtttg
    13681 acatgcagac gacattctca ggtaaattgg ttccacaaac agaagaagat attgatcaag
    13741 taatttgggt aagtccaaat gaattaccta actattttga tacgatgtat cctagtattg
    13801 ttgatgtttt gaaagaaata atatagttaa tagccaatca gccatttcca aaaaaataaa
    13861 gtaggaatcg aaattaaaat accaatggtt ggcagtgtgc tggctaattc actatctaag
    13921 tcatattcta cagctaatat tgatgccgtt atcattggcg gcatcgcgca ttcaattatt
    13981 gaaacagtag aaataatatc gtgtttttta atgatgatga tgctcattat atatacaata
    14041 aatggcgcaa tcatcagctt gtacaacaaa cctattccta attttttcca aggcaaggtt
    14101 tttgctgata ttttaaattg taagcctagt gataataaag ctaaaggtac cattagtttt
    14161 cctatattat ttagcaagat tgatggcata ccttcaataa gagaagtagg caaaaataat
    14221 gctacgataa aagcgaaaaa tggcggaaat tttaataagc gttctgtgat cgtttttatg
    14281 ctaatagtag atgcgctgaa atacgttgca acgaaaacac cacaagtaga taaaatgaga
    14341 aaactgccag gttggtcaac aaatatggca tatttcaaac tttcttcgcc atagaaatat
    14401 tgtaaaaatg gataaccaac aaacgatgta ttgcctaagc cacaacacaa tataatacat
    14461 gcaatcgttt ttttgtcaaa cataaaaaac ttagcgataa ttattgcaaa tagtattgca
    14521 ccaataaaag taatccaagc agcaccaatt ggaagtatga cttgttttga aatttctaaa
    14581 aatggaatct tcgctaatac gattgctggc aatggaatcc ataaaataaa ctgattaatt
    14641 tttttgtgta atgtagcagg tataatagaa atgaattgca aaatgaatcc aacaataaaa
    14701 agtaatatta aaaaaatagg ttgttgtaat atttccatct tatgtcaaat tcaaacagta
    14761 ttcaatgata tttttttcct tggtaaatgg cttcacagca ttcaaaccta aatagtttat
    14821 tgcctcatct attgataaag attggtgtgc aaatgcttgc ttgggaatca aaccaataag
    14881 ctcgctgccg tttgtttcca catgatgtgc ggtgcaaata ttttttattg cagtaaatac
    14941 gtcaattatc ggactttgat gtatgtctgt gatgtttgtc gatatttgcg tacaattata
    15001 agcttgcatc caccagccta ttgcttttac atgttgaaat aaattagata attttgagcc
    15061 atcattcttt gtgcgtatat ctctaatttc ttttgctatc ttttttgcta atgttaggtc
    15121 ttttgattgt aaattgatat tataagcaat cagaaagtta cgcgcaccaa gtgccattat
    15181 accaaatgtt ggatggaata tttgtggacc aaaatctggc ttccattctg ctttctttat
    15241 tttttcagca atgccttcgt attcgttttt tctaatgtac gacaagtttt gtcgtagtgc
    15301 tgtgtttgct gatttttcat ataaaaaaac aggaatacca tcatcaccta atcgtttgcc
    15361 taatttctta gacagctcaa tgacttctac taatgaaata ttttgaaacg gaattaatgg
    15421 acaaacatca caagcgccca tgcgtggatg ctcgcctttg tgcgcgcgca tgtctattat
    15481 tttttctgca actttaattg ctcgatatgc tgcttctata acggcatcag gttttccaat
    15541 aaatgtaaaa acagttctat tcgcatcaac gccaatatcg gtatgtaaca attttacaaa
    15601 tgcaacgcca cgaatagcat tggcaattgc ttcaatactt gctttatttc ttccttctga
    15661 aaaatttgga acacattcaa tgagttggtc actagtagta aacattatgc tacgaagtta
    15721 ataaaatcaa ctacattctt tacccaaatt tcagcttatc ctaatattga aaatagatag
    15781 gcgaatgctt tatagaaaaa ttaaagttaa actcttaaat aattggtatg aaattttcca
    15841 atttaatgcc aaagaatttg tatgtgcaga aggtctgtat gtataattta atcgtagtcg
    15901 cttgtgttta tgtgtctgta aaatatattt ccatacatac caaaactctt cttttacaag
    15961 cctcgtgaat tgaccattgt ataaaattat ccattttttg cttaagtttg actcttgaaa
    16021 tatgacaagc gaacttacat ttttttttca gcagtatgtg catttgccag cagatgaatt
    16081 agacgatatt acctctaaat tcacacataa aatcattaaa aaaaatgaat ttgtattgca
    16141 ggaaggtgaa gtttgtaaag acttgatttt tgtccaaaaa ggctgtttgc gcttgtttta
    16201 tatgctgaat gatgtagaag tgtcggtatg gtttgcatta aaacattcat cagcaattga
    16261 aatatatagt tttataagcg aaacgcctac gcaatatttt ttacaagcta ttgaagatac
    16321 ggaaatattg tatttgccaa aatcagcatt aaacaaatta tacgagtcgc atccattaat
    16381 gcaagaaatg atgcgcaagt tttgggaaga tgtcatattg catttgttat ctcgctttac
    16441 atcattgcag agagacactg ccgaacaacg ctatctggaa ttattaaata aaccagaatt
    16501 attgcagtca attcctcaaa aatatttagc ttcatttatt ggcgtaacgc caacatcatt
    16561 aagtaggatt aagaaaaata taaaaaaata attggcttgc aaccttttta acaaggacat
    16621 catctaccag catatataaa gcaaattttt tatttatgtt gttacaaatt caagctgcaa
    16681 gcggatttaa tcttatcgaa aaattacaaa atcttgcatt tgctttcgcg ccaaaactag
    16741 taggtgcaat tattgtttat atcgttggtg ttttcgttat taaaagatta gcgactgtgt
    16801 taaccaaaat aatgcaacgc aaaaattatg ataaatcatt acagacattt ttagtttctt
    16861 taattaaagt catgctgacc atcttgttat tactcagtat agcaggcatg ataggcattg
    16921 atattacttc ttttgcggca attttagctg gtgctggctt agcgattggt gcggcgctga
    16981 acggctcatt gggcaattta gctggtggtg tgatgatgtt ggtattcaag ccttttaaag
    17041 taggtgattt aatagaagcg caaggtgtga tgggaaaagt aatcgaacaa ggtgtatttg
    17101 caactaccat tttaactggc gataataaaa caattattat tcctaacgga ccactctcaa
    17161 caggtgtaat tacgaattat aatacacatg gaaacttacg tgtagatttg aatgtagcta
    17221 ttgcaatgac acaggatatt gatgttgcac gtgcagtagc aacagaagca atgcttagtc
    17281 atccaagagt attaaaagta ccagcaccag aagtgagtgt gttgaaaata gctgatggaa
    17341 tgtgttcttt agcgataaga ccatatactg tgcaggctga ttattgggac gtatattttg
    17401 gtgtacaaga gttggtaaaa aaggcatttg atgctaaagg tattgaaggc ccaacaccaa
    17461 cgagaatcat tattcagaaa taagtttgtt ttgtagattt ctttggaaat cgttttagaa
    17521 ggtagaaaaa gtggggtttt ctaccttctt aattttttag ctaaatatga tagcgcgggt
    17581 cgaaatcaat catccattct atgccatatt tatctctaaa cattccaaaa taggaacccc
    17641 aaggactatc gccgataggc atttctactg caccatctac tgaaagcgca ttaaatattc
    17701 tatcagcttc ttctttactt tctgcactga ttgatatctt agatctgttt tcgctttcgt
    17761 ttacttttcc cataaacgca ggaacatcat ttgccatgag cacatttttt ccaataggta
    17821 atgcaatatg cattatcttg tttgcttcat cttcttgaat ttgattgttg tgctcactcg
    17881 gcatatcttt aaaacgtata agcattgcga attcacctcc aaaaacggac ttgtaaaata
    17941 gaaacgcttc ttctgcattg ccattaaaaa tgatgtaagg attaatttgt gccatagctt
    18001 ttttattcta atttaactat aaacattgaa taagccatat tattaatccc aaagtaagat
    18061 tgatttcttg ccctatgaca actatttttt tgatgtgttg cagtaaattt gcagtataaa
    18121 atttaaaaat aaatgtcaac aacatcgcca tctatcgatt ttaacttgaa gtacaaagtt
    18181 aaagacatgt ctcttgcaga atggggcaga aaagaaatta cattagcaga agcggaaatg
    18241 ccaggtttaa tggccttacg tgaagagttc ggtccatctc aaccattaaa aggcgctcgc
    18301 attgctggtt gcttacacat gacaattcaa actgctgtat tgattgaaac tttagttgcc
    18361 ttaggagcag aagtaacttg gtcttcttgc aatattttct ctacgcaaga tcatgctgct
    18421 gctgcaattg ctgcggctgg tattcctgtt tttgcttgga aaggcatgaa cgaagaagaa
    18481 tttaattggt gcatcgagca aactttattc ttcggctcag aagacagacc attaaacatg
    18541 attttagatg atggtggcga cttaacaaac atggtatttg ataactatcc tgaattagca
    18601 aaaggaatca aaggcttgtc agaagaaact acaacaggtg ttcaccgttt atacgaaaga
    18661 atggaaaacg gcacattgtt gattcctgcg attaacatta atgactcagt tacaaaatct
    18721 aagtttgata ataaatacgg ttgtcgtgaa agttgtgtag atgctattcg tcgcgctaca
    18781 gacgtaatga ttgctggtaa agtagctgtt gttgctggtt atggtgatgt tggtaaaggt
    18841 tctgctgaat ctttgcgtgg tgcaggtgct cgtgttattg ttaccgaaat cgatccaatt
    18901 tgtgcattgc aagctgcaat ggaaggatat gaagtgaaaa aaatgattga tgctgttaaa
    18961 gaagcagata ttgttgttac gacaacgggt tgccgtgata tcattactgg tgagcatttt
    19021 aaagtaatga aagacaaaac tattgtatgt aatattggcc actttgatat cgaaattgat
    19081 gttgcttggt taaaccaaaa ttatggtcac acaaaaaata caatcaaacc acaagttgat
    19141 ttatatacta tcgaaggtaa agacgttatt ttattagcag aaggtcgctt agtgaacttg
    19201 ggttgcgcta caggtcatcc atcatttgta atgtctaatt cattttctaa tcaagtaatt
    19261 gcacaattag aattatggtc aaatgctgat aagtatgaaa acaaagtgta tatgttgcct
    19321 aagcatttag atgaaaaagt agctcgctta cacttagcta aaattggcgt agagttagat
    19381 gttttaacag cggaacaaag ctcatatttg aatattccag ctgctggtcc atataagcca
    19441 gaatactata gatactagtc ttttagtgtt attacgatgc aatttttatt gcaacataaa
    19501 aaagtcgaaa cgtagtttcg gcttttttct tttaaacaat aagatactgt atcttttgat
    19561 ttgttcctac tgtctaataa ctacatttta attgaatgag ttaaagtgtt tatcattaat
    19621 acagctaaaa ttgttataat tcattgcaac aataaaaatg atgtgcatta catatacatt
    19681 aattattttt taagtagaat ttctaagttt tgcaatttga aaaaataatc tatctttgca
    19741 ttccagttct taaaaactat acggacattg tagctcagtt ggttagagca tcggattgtg
    19801 gttccgaggg tcgtgggttc gagccccatc attgtcccta ctttttttac cgtatcaaaa
    19861 gtctttcata cattttttca attgctggta aaatatattt ccaattgata aaggcaatca
    19921 tactgcctaa tataattcca gctaatacat cacttggaaa atgaacacct acataaattt
    19981 gtgcaaatcc tattgcaatt gcccaaatga tccaacataa atttatccat cttttggtga
    20041 aaatattcgc cattacaata ctcaaagcta tagcaatatg gtttgtagca tgtgttgatg
    20101 gaaaactcca tttgccacca caatgcccta gcaataaatt tgcttgcaag tatgctgaat
    20161 gacatgggcg taatcttgca acgaaaggtt taatgatgcc tgctgaaata taatctgcta
    20221 aagcaaaagc aaggacgatg tataaaatat attttagtgc ttcgatatta tatttgtaca
    20281 acaaataaat ttctaagaaa atatataatg gaatccaatt gtttgcgtgt cgtaagaatg
    20341 gcagcaatac atctaagaag ctattgctaa tcgtatgatt aataaaatga aaaatggaat
    20401 aatcaagttg tagtaaacta tccattaaaa ctgaatttca aattcatttc tttccgtata
    20461 tagtttccaa aattgagttg cgatgttagc aggtgtatgt ttttcagcat ttggtgaaac
    20521 caaaccacat actgttacgg ttccaacaaa gatatttttt gattttaatg cttgatgcag
    20581 agaggcagca agattgcgaa tgcctgcttt tccgatagct aatgaaccat aatcgggatt
    20641 aggctcaagt gcaaagccac caccagttaa taaaatagta cctttctctt ttttttccat
    20701 gtcgggcaaa acgagatgta aagcagtcat cacactggcg acatttattt taaaatctcg
    20761 tgtaagtccg tcagcagttt ctatcgcaat atttacattt tttattttag ctacattgta
    20821 atggatggtg tctatgtgtt cccaaatttc ccatatttga ttaaaagctt cacgcatcga
    20881 ctctggatcg cctgcatcgc ctgtgaatgc atgcgcatca atgccttcag caattagttg
    20941 ctgttttaaa atttctaatt tttcactttt gcgagcgatt aacgcaattg aaaacccttg
    21001 ttttccaaat tgtgttgcca cagcttttcc aatgccttgt cctgcgccta ttattacaat
    21061 taccttgctc ataaattatg ctttctttgc caataaaata tatctatttg aaagttttgg
    21121 atgaaatgct tgaaggttat aatcgccaaa ttctttcac
//
LOCUS       DAOQQV010000035        20416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_785_length_20416_cov_8.356220, whole genome shotgun sequence.
ACCESSION   DAOQQV010000035 DAOQQV010000000
VERSION     DAOQQV010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20416
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_785_length_20416_cov_8.356220"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..987
                     /locus_tag="PLL59_05450"
     CDS             208..987
                     /locus_tag="PLL59_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMY23284.1"
                     /translation="MRKLLNNICLEAYNSKQRFTLAKHLKQLEANNTEPATFYFLRSS
                     IYSSLIEGSTIDIDRYFGNLDAGYSSREMKQVQDLVETYRFARSKALTYANLMKAHEI
                     LSRNFEMPDHYKGHLRDKEVRVGNFLTTVYVGLEVKRLAAEFELFFSELNELKQRKNY
                     TLNEAFYYASLTHLVFVKIHPFADGNGRLARLLEKWFLAQIIGDRAWKIPSEASYYLK
                     REQYYKNLQIGKNYDEIKYSMSVPFLLMLPGAFSVSKKFYK"
     gene            complement(1131..2210)
                     /gene="aroC"
                     /locus_tag="PLL59_05455"
     CDS             complement(1131..2210)
                     /gene="aroC"
                     /locus_tag="PLL59_05455"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMY23285.1"
                     /translation="MAGNSFGEIFRISTFGESHGVALGVIIDGCPAGLSIDTSFIQSE
                     LDRRKPGQSAIVTQRKEADEFEILSGIFEGVTQGTPIAIIIRNEDQKSKDYEHIKDSF
                     RPSHADFTYQEKYGTRDYRGGGRSSARETASRVAAAAIAKQFLKHYNIDVQAYVSKVG
                     DIALTQDYSTLDLTKTEDNIVRCPDVATANKMIELIKAVKKDGDTIGGCVSCVIKGVP
                     VGLGEPVFDKLHADLGKAMLSINAAKGFEYGSGFAGTSLRGSQHNDIFYNDKGVIKTK
                     TNFSGGIQGGISNGMDIYFTVAFKSIATIMQEQSSVDKDGNEVSVTGRGRHDPCVLPR
                     AVPIVEAMAALVIADHLLRSKLSKL"
     gene            2333..3985
                     /locus_tag="PLL59_05460"
     CDS             2333..3985
                     /locus_tag="PLL59_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMY23286.1"
                     /translation="MKKIVFSIVLSLFLSHIFAQTKQTTSPLNARPKLVVGIVIDQMR
                     WDFLYRYYDRYSNGGFKRLMQEGFNCENTMIPYSPTVTAAGHASIYTGSTPALHGIAG
                     NDWYDYDAQKNMYCTEDKSVKTVGATTKSGEMSPKNLLATTIGDELKLATNFKSKVIG
                     VSLKDRGSILPAGHSADAAYWYDKKTGNFISSTYYMEELPKWASAFNASNAVFNYYQK
                     NWNTLYPINTYTQSDVDTNSYEYEGFDSTRKGFPYNLTSLIDKKDYDVIFSTPYGNSI
                     TFDFAKQALINEQLGKDSITDLLAVSLSSTDYIGHAFGPNSIETEDAYLRLDKDIESF
                     LSFLDKEIGKNKYTLFLTADHGVAHIPAYLKKHNIPAGNVSGSKLRKELNVLLKEKFG
                     YDSIILASFKNQFYLNFQLIKQKDLNRDKIMETALEFLKYIPGIDRVLDYTDILESTL
                     AMEVKEQFIKGYHPKRCGDILTIYKPGYIDQKDGGTGTQHASWNPYDAHIPLLFFGYG
                     INKGKLFETTHMTDIAPTITSLLHIQMPNACIGKTISSAIKK"
     gene            complement(3989..4417)
                     /locus_tag="PLL59_05465"
     CDS             complement(3989..4417)
                     /locus_tag="PLL59_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HMY23287.1"
                     /translation="MTLWKQTVSTANIKQHKSGTIDEHLGIELIEIGDDFIKAKMPVD
                     HRTRQPMGLLHGGASVVLAESLGSLAANLCLDITKEYAVGLDINSNHLRSVRDGFVIG
                     ITKPIHIGSKTQVWEIKIYDENEQLVNISRLTMAVLQHKK"
     gene            complement(4419..5177)
                     /locus_tag="PLL59_05470"
     CDS             complement(4419..5177)
                     /locus_tag="PLL59_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HMY23288.1"
                     /translation="MEFQDIIFEQRDAIAIITLNRPQSLNALTPLMLRELKDVFFCIA
                     NDASCKVVIVKGAGRAFSAGVDLKAMNESLVGGQFTAYDIIQYGNELANIIRTMPQPV
                     IGQIHGHCYTGATELMMYFDMTIASEDLKIGDTHAKWGIMPTWGMTQRLARLVGINKA
                     KEMSFTCKAITGTEAARIGLVNKAVPLTELENAVLEMANDILLNSAQTIAALKHLYNE
                     GYHNTLEEGLKIETAYKVNITDRADFLKQFEKNK"
     gene            complement(5255..5638)
                     /locus_tag="PLL59_05475"
     CDS             complement(5255..5638)
                     /locus_tag="PLL59_05475"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMY23289.1"
                     /translation="MKDFKKYYIIHTPPEMLYLALTNPTIIKLWSGEDAEMSTAINSE
                     FSLWSGNICGKNVAFEENKKIVQEWYFGEQEPASIVTILLHAHTKGTSVELRHINIPD
                     EVYDEMVEGWNGIYFGALQDFYEEG"
     gene            5698..6345
                     /locus_tag="PLL59_05480"
     CDS             5698..6345
                     /locus_tag="PLL59_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23290.1"
                     /translation="MATSHTIQTTKTARIFTHGTLSEKTKIVWIVTHGYGFLAEYFIK
                     KFEELDGEEHFVIVPEALNRFYLKEMSGRVGASWMTTEDRENEIEDYIKYLDNVYDTL
                     ILQSKAKIVALGFSQGVATITRWAMKTNKRLNNIVIWGGNIPNDCLNDISKLNKLHPY
                     ILVGDTDQYITKEIYANVLKSLDNIGLIFSHIFYKGGHAILTEPLLQLRDELTAP"
     gene            6408..7271
                     /locus_tag="PLL59_05485"
     CDS             6408..7271
                     /locus_tag="PLL59_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HMY23291.1"
                     /translation="MKKIIASLVFTIFVFTTLFAQIEYGVASFYGTKFHGKATASGEK
                     YNQFALTAAHKTLPLGTIVKVTNAQNNKSVFVKVNDRGPFIKGRVIDLSTKAADILGY
                     RNKGTAYVKIEIIDKDEQPADLMEATENIAEQNGIKTYDSTANTTISDMTNWKEIPTV
                     NTEPSSDNAITAKKESAPNPIDAIISDANSNATTNRSPYYIITKLDKSKSGFYGLQLG
                     VYSDMNVILAIIADLEAKYHQTLLVEQVDLNGKTVYKLFIGKFQNRAYADALKSVIAD
                     KYKDAFVLKYE"
     gene            complement(7392..8975)
                     /locus_tag="PLL59_05490"
     CDS             complement(7392..8975)
                     /locus_tag="PLL59_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HMY23292.1"
                     /translation="MSAFTYDYVIIGSGFGGSVSALRLSEKGYKVLVIEKGKWLKADD
                     FPETNWNLRKFFWLPALKFQGLFKLTYYRHVAIVSGVGVGGGSLVYANTLPIPKTEFY
                     NSGNWNGLENWEEELKPFYTTALKMLGAERHPYMSRNDKAMQQLSEKIGCPEKFDKTN
                     VAVYFGKVGVTVPDPYFDGKGPDRTGCNLCGGCMLGCRFNAKNTLDKNYLHLAQQLGA
                     TIIAEKEVYDVVPLSADGGDGYEIKFKDSLSYFPKKDSIKAKGVIFAGGVLGSVDLLL
                     KMKDSSLPNLSNKIGAGVRTNSESLIGVTTYEKDVSFSEGIAIGSIINIDDHTHLEPV
                     KYSEGAGFWRLLMAPMVSGSNIAVRFYRMVKDFVMHPINNFKIFFVDDWSKKTQIMLY
                     MESIDSTLSFARGWFGRMKTKLESGPAPTAFNPKAQELAREMETIINGKAMVMNTETL
                     FGIPTTAHILGGACMGKSAEDGVIDKDNRVFNYKNMLVCDGSMVSANPGVNPSLTITA
                     ISERAMTKIPMKSDNLDFK"
     gene            complement(9067..10011)
                     /locus_tag="PLL59_05495"
     CDS             complement(9067..10011)
                     /locus_tag="PLL59_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMY23293.1"
                     /translation="MSIEHFDSIYQSFPGLSAEEWKAKICKDLKGDSFEKLIWHTNEG
                     FDVLPFYTKEDNKNYQLNIPKRLTNGWQIAERIVVNNIETANAQALLALENGATSIFF
                     DVQHQSLSQNDVFGLVKNILLDIAPVTFENFNSNDKYVFESIVNNSCTPKVSVPKLEN
                     ITEELVFALQEGIQQKTNSFHFLITSNYFFEIAKLRAFRWLWKQASTVHQLPTNANII
                     AETCLNNLSKNEENTNLLRNTTMAMSAILGTCDVLLVQSHNAEDNGFAKRMARNIQHI
                     LQHESNFDKMQDAAKGSYYIEYLTYQFCMQSWNKFTAQ"
     gene            complement(10040..13732)
                     /locus_tag="PLL59_05500"
     CDS             complement(10040..13732)
                     /locus_tag="PLL59_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY23294.1"
                     /translation="MKPLISLFLYIVIAMSTNLFAQVQWASKLIDFSSEYKSELIQAN
                     STRCSAAQVLGLPNVMKYGESSLAWSPAKQQAGKEFVTVGFATPQTVQQIVIGETYNA
                     GSICEIILYDNNGKKYTVYENKNPSFVKKYNDLLSYFKIKPVYNVNKLKLVLNTDAVG
                     GGQEIDCIGILSSTAPYQHQVNVIKYSETVGIPENLGPNINSSYYDHLPLISPDGSML
                     YFIRKLTNEDNTQRFDDDIYLSHIMPTGKFTNAENIGPPLNNQTSNFVCFISKDNNRL
                     YVANKYIKNTYNYSGLSVSSKQKNGDWSKPKPLSIPDLYNKNEFAHYHMNLDENIALM
                     CIQRDDSYGDLDIYVSQKYSDGDWSKPKNIGPVINTVGAEGSVFLAADNKTLYFSSTG
                     HAGFGSYDMFMTKRLDDTWTNWSKPINLGNKINTDEMDIYYTIPAAGDYAYFSSGQSY
                     FGKNDLYRIKLPKEARPEFVDVAKVLASSPIQKNTIPTYASNTNIATTPTKTTQPTTP
                     PKSTVTPISSVNKTTDELQNKLEELKKQQAQATRPTTTVVAQQPKQEITPVSNVPSPT
                     TSIKASVSTTMPTSTVQVKKEVVNYIEPYKTPEELRQKPATTNTIKPAEMPKTSPHVL
                     TKEELAAREQQKSNPTINPANDNLTNLTKRPQPEIIPTSNVLSTSSPQIQNPTIVTTE
                     VEPVATPATTISSVPSSVNLRTSQVATDDLQQKLDALKQQQSAIQQPSTTTYTTPTST
                     ISVREQYPNPSPVLPTSNAKTDDLQRKLDELKQQQNQVGQSNKIYANPYEQKPYGAQA
                     PKERQEDMQSVAYDDYKKKLDDLKKQQQSVSVPVNTTTSVNTPPAKLNDNNITPSLSK
                     NIIETTPTIANNTSEPNPILSKYEDKLRKMKEEMAALNATPPTDTKLDAVASTPISNT
                     TTNASAEPESVADDKKAIVIDKFENQDEKQNTLTNTSPKETIVVSVPAIDFELEKAKL
                     DSFQQAQKAEEQKMLASIDKMGANKQTLENDIADLKAQQKKFNEENDKLTTQNSQLAS
                     ERDKQAIEKKKMEDLLAQMQEERDKLAAEKLKMEQDKAKLELLKKQQEKDVFTLKRSI
                     DSLSKIQQKTANTSTTPQNYDLFNVPLQVGAVAQVNNIYFVADASFLQIPSYPELDKV
                     ALFLARNQKLKVEIGGHTNGLCDDFFCQKLSTSRAKTCVDYLISKGIASNRLTYKGYG
                     KSYLLKPAEPGNSLNQRVEIKILSMD"
     gene            13888..14790
                     /locus_tag="PLL59_05505"
     CDS             13888..14790
                     /locus_tag="PLL59_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23295.1"
                     /translation="MKIFFCSILLAISFISQAQITPDNHRQLKLYEDTIRMYGDTLIN
                     SKLKDNRTIASYRIIKTLTKALRIENSYYYSWDSLLPWKIIQAEDGAFKIFTWYAQSD
                     EGMYKFFGTLQMKSDTLKMFPLIDYSEFTDHQQDINVDNNNWIGALYYGIKTVKSGKK
                     NYYTLFGWDGNNMMSNKKVMEILSFNKQGQPRFGAPIIDMGNGKILNRFILEFAKEAS
                     ASLNYNATEKRIIYDHIAPNGNSLEGFFANYVPDGTYEGFVWKKGKWRHVENIEYEKR
                     TDGDVPNVGKVREFELYQPKKSGK"
     gene            complement(14792..15496)
                     /locus_tag="PLL59_05510"
     CDS             complement(14792..15496)
                     /locus_tag="PLL59_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMY23296.1"
                     /translation="MLSQNEIKHINSLKIKKYRKKYLEFIAEGDKIIKELIDEGMEVV
                     HIFAINDNFIHQKCRIISESDLNKITALQHHHNSFAVFKIPIIDVSINSNEWSLVLDD
                     IQDPGNMGTIIRTCDWFGIKQIFCSDGCVDAYNPKVVQASMASVGRVNVIETDIEQLF
                     MEHDLPVYGAVLNGENYRVQTYEQKGFILIGNEGHGIRESLQAKITFPITIPKNGKAE
                     SLNAGIATALILDKLV"
     gene            15498..18050
                     /locus_tag="PLL59_05515"
     CDS             15498..18050
                     /locus_tag="PLL59_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMY23297.1"
                     /translation="MQNSFQRCKKLGYVVCIALLYSCSATKHLIKDDQSLLISNGVSI
                     KYSNKVVSDKTSLKSELAKQVVYNQQPNKRFISLFRLKLGVYTMNILRNEKKEARKKE
                     LETKIANNTHTAKDLSKLKKLEKKRRKKISANKTNNGEAPVLFDSTTIATSIKRMKNY
                     LFYHGFFYSDVTSSFKTKNKKTSVTYHINTGPQFTYRKIHLTALDSILLKIVEKEEKK
                     SLLKKNDPFDIDIIKKERQRIAETIQNKGYFTFSPDYIFLNIDSTIGNHQIDVNLIVN
                     NEADSLTHKLYSYVQIDYDILNFDNKKENKKLTRKDFIYDTICDVNYRVLKTSVLPRA
                     LTKSTYIKPGDLFNKDQLLKTRNALNSLGVFRFVNIEHIPISISPEESGLYTRIKCTP
                     AKRHAFGTDVELNTNAQSTLGFNLVGSYRNNNLFRSAAKFQFNVSTGLEFQLLKEKRL
                     ENKAVNTVNFNMEAKLNLARIFPSFKKNKCVTYQKYKPRTFISLNYNFQRRIGLYNIQ
                     TVGTNYGYEWYNEKYRHIFSPLSFTYVIPSRISDSFQTELNKNARLQQSFSQQFILGQ
                     EYTFSYTNQDLNVGKYKNYFYFRANAFLAGNFLFAFSKAAQKNAVTPYKLGKVNFAQF
                     LRLEMEPRYFFNFRRGQALSFRTFIGIGIPYGNSRYTTSVAINDSTFTQRDVAVIPYI
                     KQFFAGGPNSLRGWGYRQLGPGGFNTHNKNRNNLDQTGDLKFEFNAEYRFNIYKIFKG
                     AIFTDIGNVWLIKEDPDKALANFDTKRFMKELAWDIGIGLRMDLNFFVVRLDLGYALY
                     DPAFNEGDRWTFNKINNDDYLIFKQPRNNDIGFYKFSFKDFLGINFAIGYPF"
     gene            18087..20330
                     /locus_tag="PLL59_05520"
     CDS             18087..20330
                     /locus_tag="PLL59_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23298.1"
                     /translation="MRNAIIDYNRYIQQTCILLSLYFMIQANSILAQQYFRFDYLPVK
                     SNNTSLQYPWTGGINNAQFGMVDVNHDNRKDLVVYNKSNNNFLIFTSTQNNSTQYHFS
                     SKYAAHFPPVNGWFIMKDYNCDGIEDIFTYNNVANIKVYTAYYANDTLKFKLQQDGFF
                     YQGVSGKINLYCSDVIKPALADVNKDGDLDVISFNVFGNRLQYYENQQKELALPCDSL
                     FFTKADNCWGNVLDTLAAAYALNDTCTFKFNRPNSNENIMHTGSSLAAIDIDANETTD
                     LAIGSIGLNSLTLLYNLGTKNYASIQQQDITFPNYNIPFDVSSFGSPSFVDANNDGFT
                     DMLVSTFDVGTANINNIWYYKNTKTSGTPSIELALQQKNFLLENMIDAGENSYPCFFD
                     ADGDGLKDILLGSGGFKDYVQAAVYKLQLYKNIGNAEQAAFELIDDDYLGVSTLNVKD
                     LAPAIGDIDNDNDTDIVVGLSDGKLIYWENTAITGNAPNLLYKGLLKDATGIAINVGA
                     NATPCLVDINRDGKTDLIIGERNGNLNYYTGNSIDSIDFAFVTDSLGKIRIKTNGLSI
                     GFTQPTIADINNDSKYDLLLGTNISGLLKYYNIEDNLTGTFISSGNVVSESIGMRTSS
                     TIADISNDTKAELLCGNIAGGLTIFSQFPPPIIPSTIYEKRLEEIAFSVFPNPANHSI
                     SVSLKAHFSNISVQIINTIGQEIFHQTYSNINTFEINTSTFNKGLYLLKINDGKNIGF
                     QKILIQH"
BASE COUNT         6737 a         3626 c         3410 g         6643 t
ORIGIN      
        1 gttaaatcaa ttcacaacag aactaatgtt gaaagaagaa caaaacatgt tgaaagaacg
       61 gtgagcgcga ccgccgcaac ggaaaggatt tttacacatt caccaaaaaa attaaaattg
      121 actaatcaat aagaatacaa acaaaatttg gtatacaaac taatataaag cactctcctg
      181 tatggctttt tagtatattt gctatgcatg agaaaattat tgaacaacat ctgtttggaa
      241 gcatacaaca gtaaacaacg gtttacgttg gctaaacatt tgaagcagct ggaagcgaat
      301 aataccgagc ctgctacttt ctattttctc cgttcttcaa tctattcctc tttgatagaa
      361 ggcagcacca ttgatataga cagatatttc ggcaacctag atgctgggta cagcagccgg
      421 gaaatgaaac aagtgcagga tctggtggaa acatacagat ttgcaaggtc gaaagcactg
      481 acatacgcaa atcttatgaa agctcacgaa atattgagcc gaaatttcga aatgccagat
      541 cactacaaag gtcatcttag ggacaaggag gtgcgcgtcg gcaatttttt gacaacagta
      601 tatgttgggc tggaagttaa aagactggcc gctgaatttg aactattttt ttccgaactc
      661 aacgaattga agcaaagaaa aaactatacg ctgaacgagg cattctatta cgcatcactc
      721 acacaccttg tgttcgtcaa aattcatccg ttcgcagatg gcaatggtcg tcttgcccga
      781 ttgctagaga aatggttttt agcccaaata ataggcgaca gagcctggaa aatcccaagt
      841 gaagcttctt actacctcaa aagggaacaa tactataaaa acctgcagat agggaaaaac
      901 tatgatgaaa taaaatacag catgtccgtt ccgttcctac taatgcttcc tggtgcattt
      961 tccgtatcca agaaattcta caagtaagct ccctaatgtt tttattggca tagaaaaaat
     1021 tggaaccaat atataaagaa cacaatttaa ttgctctaaa aaagatatcg tcaaaaaata
     1081 aaagaaggaa aatcatattt tggaaattaa agcaaatcgc ttgtatttac ttacaattta
     1141 ctcagtttgc tacgcaataa atggtccgca attacaagtg ctgccatcgc ttcaacgata
     1201 ggcactgcgc gaggcaagac acatggatca tggcgaccgc gccctgtaac gctcacttca
     1261 tttccgtctt tatccactga gctttgttct tgcataattg tagcaataga tttaaatgca
     1321 accgtaaaat aaatatccat accattactg atgccacctt gtatgccacc agaaaaatta
     1381 gtctttgttt ttattacgcc cttgtcgtta taaaatatat cattgtgttg tgagccacgt
     1441 aatgatgtac cggcaaagcc acttccatat tcaaatcctt tagctgcatt aatactcagc
     1501 attgcttttc ctaaatcggc atgtaattta tcaaaaacag gctcgcccaa tccaacagga
     1561 acacctttta taacacaact tacacaacca ccaatagtat cgccatcttt ttttactgct
     1621 ttgattagtt ctatcatttt atttgctgtg gcgacatctg gacagcgaac gatattgtct
     1681 tctgtttttg tcaaatctaa tgttgaataa tcttgtgtca atgcaatatc gccaactttt
     1741 gaaacgtatg cttgcacatc aatgttgtaa tgttttaaaa attgtttggc aatagcagca
     1801 gcagctacgc gcgatgccgt ttcgcgtgct gaacttcttc caccgccacg ataatcgcga
     1861 gtgccatact tttcttgata ggtaaaatca gcatgagatg gacgaaagct atcttttata
     1921 tgctcataat ctttcgattt ctggtcttca tttcgaatga taatggcaat tggcgttcct
     1981 tgtgttacac cttcaaaaat tccagaaaga atttcaaatt catcagcctc tttgcgttgt
     2041 gtaacaatcg cagactggcc tggtttgcgt ctatctaatt ccgattgaat aaacgaagta
     2101 tctatagata aaccagccgg acaaccatca ataataacac ctaatgcaac accatggctt
     2161 tcgccaaatg tacttattct aaatatttcg ccaaaagaat ttcctgccat acaacgaaga
     2221 tagtggtttt ttggtgtata gcattttaac gaattaagaa ttattggcga ataatatcat
     2281 tttcgatgtt tcattctatt caaattttat aacttgcata caaatattag aaatgaaaaa
     2341 aattgtattt tccatagttc tatctttatt ccttagccat atatttgctc aaaccaaaca
     2401 aacaacatca ccacttaatg cgcgtccgaa attagttgtt ggcattgtta tagatcaaat
     2461 gcgatgggat tttttgtatc gctattatga cagatatagt aatggcggat ttaagcgttt
     2521 gatgcaagaa ggattcaatt gcgaaaatac aatgattcca tatagtccaa cagtaactgc
     2581 ggctggacat gccagtattt acacaggaag cacgcctgca cttcatggca ttgccggaaa
     2641 tgactggtac gattatgatg cgcaaaaaaa tatgtattgt acagaagata aaagtgtaaa
     2701 aactgttggt gcaacgacaa aatcgggtga aatgagtcca aaaaatttat tggcaactac
     2761 gattggagat gaactaaaat tagcaacaaa cttcaagagt aaagtaattg gcgtttcgct
     2821 aaaagataga ggctccattt tacctgcagg tcatagtgct gatgcagcat attggtatga
     2881 taaaaaaaca ggaaatttta tttcttcaac atattacatg gaagaattac caaaatgggc
     2941 aagcgcattt aatgcgtcga atgcagtctt taattattat caaaaaaact ggaatacgct
     3001 gtatcctatc aacacctaca cgcaaagtga tgtggacact aattcctatg aatacgaagg
     3061 gtttgacagc acgcgcaaag gatttccata taacttaaca agcttgattg acaagaaaga
     3121 ttatgatgtc atttttagca caccttatgg aaattctatc acatttgact ttgccaaaca
     3181 ggcattaata aatgaacaat taggaaaaga ttctatcact gatttattgg ctgtttcact
     3241 ttcttcgaca gattatattg gtcatgcatt tgggccaaac tcaatagaaa cagaagatgc
     3301 ttatcttaga ttagataaag acatagagtc atttctatca tttttggata aagaaattgg
     3361 caagaacaaa tacactttat ttcttactgc cgaccatggt gtggcacata ttccagccta
     3421 tctaaaaaaa cataatattc cggcaggaaa tgtatcgggt tcaaaactta gaaaagaatt
     3481 aaatgtgttg ctaaaagaaa aatttggtta tgacagcatt atcttggcta gcttcaaaaa
     3541 ccaattttat ttgaattttc aattaatcaa acaaaaagat ttaaacagag ataagatcat
     3601 ggaaacagca ttggaattct taaaatatat tccgggaatt gatcgtgttt tggactatac
     3661 tgacatacta gaatccacct tagcaatgga agtaaaagag caatttataa aaggctatca
     3721 tcctaaaaga tgcggtgata ttttaacgat atacaagcca ggatatattg atcaaaaaga
     3781 cggtggcaca ggcacgcaac atgcttcttg gaatccgtat gatgcgcata taccattgtt
     3841 gttttttggc tatggcatca ataaaggaaa actctttgag acgacacaca tgacagacat
     3901 cgcacccacg ataacaagtc ttttacatat acaaatgcca aatgcatgta ttggaaaaac
     3961 gataagtagt gcaataaaaa aatagctttt attttttatg ttgtaataca gccattgtca
     4021 atcttgaaat gttgacaagc tgttcgtttt catcatagat ttttatttcc caaacttgcg
     4081 tcttgctgcc tatgtgtatt ggcttcgtta ttcctatcac aaatccatcg cggacactgc
     4141 gcaaatggtt ggaattaata tctaagccaa ctgcatattc cttagtaata tctaaacata
     4201 aattagctgc caaactgcct aaagactctg ctaatactac gcttgcgcct ccatgcaaca
     4261 aacccatcgg ttggcgtgtt cgatgatcga caggcatttt agctttgata aaatcatcgc
     4321 caatttcgat aagctcaata cctaaatgct cgtctattgt gccactttta tgttgcttga
     4381 tattagccgt actgacggtt tgtttccaga gtgtcatctt atttattttt ttcaaattgt
     4441 tttaaaaaat cagctctgtc tgtaatgttt actttgtaag ctgtctcaat ttttaatcct
     4501 tcttctaatg tgttatgata tccttcatta tataaatgct taagtgctgc aatagtttga
     4561 gctgaattta gtagaatatc atttgccatt tccaatactg cattttctaa ttccgttaat
     4621 ggcacagctt tgtttacaag accaatacgt gctgcttccg ttcctgtgat tgctttgcag
     4681 gtgaatgaca tttctttagc cttgttaatg ccaaccaatc ttgctaatcg ttgtgtcatt
     4741 ccccaagttg gcatgattcc ccatttagcg tgtgtatctc ctattttcag gtcttctgag
     4801 gctattgtca tatcaaaata catcatcaat tcagttgcac ctgtgtagca gtgtccgtga
     4861 atttgaccga tgacaggctg tggcatagtg cgaataatat tggctaattc atttccatat
     4921 tgaataatat catatgctgt aaattggcct cctactaatg attcattcat cgcttttaaa
     4981 tcaacgccag cagaaaaagc acgtcctgcg ccttttacaa taacgacttt gcacgaagca
     5041 tcatttgcta tacaaaaaaa tacatctttt aattcacgaa gcattaatgg tgtaagcgca
     5101 tttaaagatt gtggtctgtt aagtgtaatg atagcaattg catctctttg ttcaaatatg
     5161 atatcttgaa attccataat taatattgat gtagaagtta gaaagaattt attgaaagaa
     5221 tattatctaa agcaaaaaat aaacaagaag cttattatcc ttcttcgtaa aaatcttgaa
     5281 gcgcaccaaa gtaaatgcca ttccaacctt cgaccatttc atcataaact tcatcaggaa
     5341 tattgatatg tctcaactct actgatgtgc cttttgtgtg tgcgtgcaat aaaatggtaa
     5401 cgatagatgc tggctcttgc tcaccgaaat accattcttg tactattttt ttattttctt
     5461 caaacgctac atttttacca caaatatttc cagaccataa agagaattct gaatttattg
     5521 ctgttgacat ttccgcatct tcgccactcc ataattttat aatcgttgga ttggtaagtg
     5581 ctaagtacaa catttctggc ggtgtatgta tgatataata ttttttgaaa tctttcatat
     5641 tgctaattat ccgaaaaaat actctttttt aattaatttg gaagaaatat ttttctaatg
     5701 gctacgtctc acaccattca aaccacaaaa acagcacgca tatttacaca tggcacatta
     5761 agtgaaaaaa caaaaattgt gtggatagtt acgcatggtt atggattttt ggctgaatat
     5821 tttataaaaa aatttgaaga acttgatggt gaggagcatt ttgtaatcgt accagaggca
     5881 ctcaatcgtt tttatttgaa agaaatgagt ggcagagtgg gcgccagctg gatgaccaca
     5941 gaagataggg aaaatgaaat cgaagactat attaaatacc tagataacgt ttacgacaca
     6001 ttaattttac aaagcaaagc aaaaattgtg gctttagggt tttcacaagg tgttgctacc
     6061 attacaagat gggcaatgaa aacgaataaa cgcctgaaca acatagtaat ttggggtgga
     6121 aacataccaa atgactgttt gaatgacatc tcaaaactca acaagttaca tccttatata
     6181 ttggtaggtg atacagatca atatattacg aaagaaatat atgcaaacgt tttaaaatct
     6241 ttagataaca ttggcttaat attttcacat attttttaca aaggtggtca tgctatatta
     6301 actgaacctt tattacaact ccgtgacgag ttaacagcac cgtaaaagtc tggcagtatc
     6361 tttgcaacga gcttttaagg caaaaaaagc ttgtaaaaaa gatggatatg aaaaaaataa
     6421 tagccagttt agtttttacg attttcgttt tcactacttt attcgcacag attgagtacg
     6481 gtgttgcttc attttatgga acaaaattcc atggcaaagc aactgcaagt ggcgagaaat
     6541 ataatcaatt tgcattgacc gcagcacata aaacacttcc tttgggaaca atcgtaaaag
     6601 taactaacgc acaaaacaat aaatccgtat tcgtaaaagt aaatgataga ggtcctttta
     6661 ttaaaggacg tgttatagat ttatctacaa aagcagcaga tatattagga tatcgcaaca
     6721 aaggtactgc atatgtaaaa atagaaatta ttgataaaga tgagcaacca gcagacttga
     6781 tggaagctac agaaaatatt gctgaacaaa atggaattaa aacgtatgat tctacagcaa
     6841 acactactat atctgacatg acgaattgga aagaaattcc aacagtgaat acggagccga
     6901 gtagtgacaa tgctataacg gctaaaaaag aatcagcacc caatcctatt gatgctataa
     6961 taagtgatgc caattcaaat gcgacaacga atcgttcgcc ctattatatc attacaaaat
     7021 tagataaatc aaaatctgga ttttatggtt tgcaattagg cgtttattcc gacatgaatg
     7081 tgattttagc catcattgct gatttagaag caaagtatca tcaaacatta ttagtagagc
     7141 aagttgacct aaacggtaaa actgtgtata aattatttat cggaaaattc caaaatcgcg
     7201 cgtatgctga tgcgttgaag tcggtaattg ctgacaaata caaagatgcg tttgtattga
     7261 aatatgaata atcattcagt atttggaact aaatttttct cactgttgtt gattacaaca
     7321 atacaataaa ccaccaagca agcacatctt gatacattgt ggcagtacgc tgtcggaatg
     7381 tatcaagatg tttatttaaa atctaaatta tcagatttca ttggtatttt agtcatagca
     7441 cgttcactta tggcggtgat tgtcaagcta ggatttacgc caggattggc agataccata
     7501 cttccatcgc acaccaacat atttttataa ttaaacactc gattatcttt atcaataaca
     7561 ccatcttcgg cagattttcc catacaagca ccgcctaaaa tatgagccgt tgttggaata
     7621 ccaaataagg tttctgtatt cattaccatc gcctttccat ttataatggt ttccatttca
     7681 cgtgccaact cttgtgcctt tggattaaat gcggttggcg ctggaccaga ttctaatttt
     7741 gttttcattc taccaaacca accacgtgca aaactcaatg ttgaatctat actttccata
     7801 tacaacatga tttgtgtttt cttgctccaa tcgtcgacaa aaaatatttt aaaattattg
     7861 attgggtgca ttacaaaatc tttaaccatg cgataaaaac gaacagcaat attgctacca
     7921 ctcaccattg gcgccatcaa caaacgccaa aatccagcac cttcagaata tttcacaggt
     7981 tctaggtgcg tatggtcatc tatattaatg atagagccaa tagcaatacc ttccgaaaac
     8041 gaaacatctt tttcataggt agttacgcct attaaacttt ctgagtttgt acgaacgcct
     8101 gctcctattt tatttgataa atttggcaat gagctatctt tcattttcaa taatagatca
     8161 acgctaccca gcacaccacc agcaaatatt acgcctttgg cttttatcga atctttttta
     8221 ggaaaataag ataaagaatc tttgaatttt atttcatatc catcgccgcc atctgcactt
     8281 aatggcacaa catcatacac ttctttttct gcaataatag tagcgcctaa ttgttgcgct
     8341 aaatgtaaat aatttttatc taaggtgttc ttagcattaa atcgacaacc taacatacaa
     8401 ccgccgcata aattacaacc agttctatct ggtccttttc catcaaaata aggatctgga
     8461 actgtcacgc ctacttttcc aaaatagaca gcaacatttg ttttatcaaa tttttcagga
     8521 caaccaattt tctctgataa ttgctgcata gctttatcat tgcggctcat gtatggatgt
     8581 cgttctgcgc ctaacatttt tagtgctgta gtataaaatg gctttaattc ttcttcccaa
     8641 ttttccagtc cattccaatt tccagaatta taaaattctg tttttggaat aggcaatgta
     8701 ttcgcatata ctaatgaacc gccgcctacg ccaacaccac ttacaatggc aacatggcga
     8761 tagtaagtaa gcttaaacaa gccttgaaac tttaatgcag gtaaccaaaa aaacttacgt
     8821 aagttccaat ttgtttccgg aaaatcatct gcttttagcc attttccttt ttcaattact
     8881 aatactttat agcctttttc cgataggcgt agtgcagaaa cagaaccgcc gaaaccgctt
     8941 cctattatta cataatcata agtaaatgct gacatagtca atttaattta gtatcatcaa
     9001 taaaatgaaa ttacaactaa gaaatgaatt tcttattata aaaagataag aagtagtatg
     9061 tcgtgattat tgtgctgtaa atttattcca gctttgcata caaaattgat acgttaggta
     9121 ctcaatataa taactacctt ttgcagcatc ttgcatctta tcaaagtttg attcatgttg
     9181 tagaatatgt tgaatattac gcgccattcg ttttgcaaat ccattatctt cagcattgtg
     9241 agactgcact aacaatacat cacaagtgcc tagtatagca ctcatcgcca tcgttgtatt
     9301 gcgtagcaaa ttcgtattct cttcattttt acttaaatta tttaaacacg tttcagcgat
     9361 gatatttgcg ttcgttggaa gctgatgtac tgtactagct tgtttccaca accaacgaaa
     9421 tgcacgcaat tttgcaattt caaaaaaata atttgatgta attaaaaaat gaaaactatt
     9481 tgtcttttgt tgaatgcctt cttgtaaggc aaacactaat tcctcagtaa tattttccaa
     9541 tttaggcaca cttacttttg gcgtacaact attatttaca atgctttcga atacatattt
     9601 atcattacta ttgaaatttt caaaagtaac gggcgcaata tctaaaagaa tattctttac
     9661 taaaccaaaa acatcatttt gtgataaaga ttgatgttgc acatcaaaaa aaattgaagt
     9721 agcgccattt tcgagcgcaa gtaatgcctg cgcattggct gtttcaatat tatttacaac
     9781 aatccgttca gcaatttgcc agccatttgt caatcgcttt ggaatgttta attgatagtt
     9841 tttattgtct tcttttgtat agaaaggtaa aacatcaaat ccttcgtttg tatgccaaat
     9901 cagtttttca aaagaatcgc cttttaaatc tttacaaatt ttagctttcc attcttctgc
     9961 gctaaggcca ggaaaagact gataaatact atcaaaatgt tctatagaca tgttacaaag
    10021 atattaaaat taaacgacat caatccatgc ttaatatctt aatttcaacg cgttgattaa
    10081 gcgaatttcc aggctcagca ggttttagca aatatgattt tccatatcct ttataagtca
    10141 atctattgga tgcaatacct ttactgatta gataatcgac gcaagtcttg gctctactag
    10201 ttgataattt ttgacagaaa aaatcatcgc acaaaccatt tgtatgtccg ccaatttcaa
    10261 ctttcagttt ttgatttctt gctaaaaaca aagccacttt atctaattct ggataagatg
    10321 gaatttgtaa aaatgaagca tctgcaacaa aataaatatt atttacttgt gccactgcac
    10381 ctacttgcaa aggcacatta aacaaatcat aattctgagg cgttgtactt gtattggcag
    10441 ttttctgttg tatttttgag agagagtcga tagaacgctt cagtgtaaaa acatcttttt
    10501 cttgttgttt tttcaataat tccaacttcg ctttatcttg ttccatcttt aacttttcag
    10561 cagcaagttt atcacgttct tcttgcattt gcgcaagcaa atcttccatt tttttctttt
    10621 ctattgcttg tttatcacgc tcactggcta gttgagaatt ttgagttgta agcttgtcat
    10681 tctcttcatt aaattttttc tgctgtgctt tcaagtctgc aatatcattt tctaatgttt
    10741 gtttgtttgc tcccatttta tcaatacttg ctaacatttt ttgttcctca gctttttgag
    10801 cttgttggaa agaatctaat tttgcttttt ccaattcaaa atcaatagca ggaactgaaa
    10861 caacaatagt ttcttttggc gaagtatttg taagtgtatt ctgtttttca tcttgattct
    10921 caaatttatc aatcactata gcttttttgt catcagcaac actttcaggc tcagcagaag
    10981 catttgtagt agtgttagaa attggtgttg aagcaacagc gtctaactta gtatccgttg
    11041 gtggtgtagc atttaatgca gccatttcct ctttcatttt tctcaattta tcttcatact
    11101 ttgatagtat tggattgggt tcagaggtat tatttgcaat tgtaggtgtt gtctcaatta
    11161 tatttttaga aaggcttgga gtaatattgt tatcgtttag ttttgctggt ggcgtattca
    11221 ctgaagtagt agtatttaca ggcacactaa cagattgttg ttgttttttt agatcgtcta
    11281 attttttctt ataatcatcg taagccacag attgcatgtc ttcctgcctt tctttaggcg
    11341 cttgagcgcc ataaggtttt tgctcataag gatttgcata gattttatta ctttgaccca
    11401 cttgattttg ctgttgtttt agctcgtcta atttgcgttg cagatcatcg gtttttgcat
    11461 tcgatgtagg caaaacaggt gatggatttg gatattgctc acggacactt atagttgaag
    11521 taggtgttgt gtaagtagtc gtgcttggct gctgtattgc agattgttgt tgttttagtg
    11581 catctaattt ttgttgcaaa tcatctgtag ctacttggct ggtgcgtaaa tttacagatg
    11641 atggaacact ggatattgtg gtagcaggtg ttgcgacagg ctcaacctct gtagtaacaa
    11701 ttgttgggtt ttgaatttgt ggcgatgatg ttgacaacac attacttgtt ggtattattt
    11761 ctggctgtgg tctttttgtc aaattagtca aattatcatt cgcaggatta atagtaggat
    11821 ttgatttttg ttgctctcga gcggccaact cttcttttgt caatacatgt ggcgaagttt
    11881 tcggcatctc tgctggctta atagtatttg ttgtcgctgg cttttgacga agttcttctg
    11941 gtgttttata aggttcaata tagttgacta cttctttctt tacttgtact gttgaagttg
    12001 gcatagttgt tgaaactgat gcttttatcg atgttgttgg cgatggtaca tttgatactg
    12061 gtgttatttc ttgcttaggt tgttgtgcta ctactgttgt cgtaggtctt gttgcttgag
    12121 cttgttgttt ttttaattcc tctaatttat tttgaagttc gtctgttgtt ttatttactg
    12181 atgatattgg agtaacagta gatttaggtg gtgttgtagg ctgtgtagtc tttgttggag
    12241 ttgtagcaat atttgtattt gaagcgtatg tcggaattgt atttttttga attggtgatg
    12301 aagccaatac tttagcaaca tcaacaaatt caggtcgtgc ttcttttggc aatttgattc
    12361 gatacaagtc atttttgccg aaataacttt gtccactaga aaaataggca taatcacctg
    12421 ccgctggaat ggtgtaataa atatccattt cgtcagtatt gattttattt ccaagattaa
    12481 ttggtttgct ccaatttgtc catgtatcat ctaatcgttt ggtcataaac atatcgtagc
    12541 taccaaatcc agcatgacca gtagaagaaa aatagagcgt tttattgtcc gctgctaaaa
    12601 acacagaacc ttctgcacca accgtattga tgacagggcc aatgttttta ggctttgacc
    12661 aatcaccatc actatatttt tgtgatacat aaatatctaa atcaccataa gaatcgtcgc
    12721 gctgaatgca catcaatgct atattttcat ctaaattcat atgataatgt gcaaactcat
    12781 ttttattgta taaatcagga attgacaaag gttttggctt gctccaatca ccattttttt
    12841 gtttgcttga aactgacagt ccagaataat tgtatgtatt tttaatgtac ttattagcca
    12901 cgtacaatcg attattatct ttactgataa aacaaacaaa attactagtt tgattattca
    12961 atggaggtcc aatattttcg gcatttgtaa atttacctgt tggcataatg tgtgataaat
    13021 aaatatcatc atcaaaacgt tgtgtattgt cttcattcgt cagtttgcga ataaaatata
    13081 acatgctacc atcaggcgaa atcaatggca aatgatcgta ataagatgaa ttaatatttg
    13141 gtcctaaatt ttctggaatg cctactgttt ctgaatattt aatcacattt acttgatgtt
    13201 gatatggcgc agtacttgat aaaataccaa tgcaatcaat ttcttgtcca ccaccaacag
    13261 catcggtatt taatacaagc tttaatttat ttacattata gacgggtttt attttaaaat
    13321 aacttagtaa gtcgttatac ttttttacaa atgatggatt tttattctca tatactgtat
    13381 attttttccc attattatca tataaaatta tctcacaaat agagcctgca ttatatgttt
    13441 cgccaattac aatctgttgt acagtttgtg gtgtggcaaa ccccacggta acaaactcct
    13501 tgcctgcttg ttgtttggct ggcgaccacg ccaaagatga ttcgccatat ttcatcacat
    13561 taggtaagcc taaaacttga gcagcactgc atcgcgtaga atttgcttgt atcaactcac
    13621 ttttatattc ggaggaaaaa tcaattagct tactcgccca ttgcacttgt gcaaataggt
    13681 ttgttgacat ggctataaca atatataaaa aaagagaaat gagtggcttc attttgtatg
    13741 tttacgattt tgtttcaatt tgaaatataa taaaatacag ctacttttaa atattattaa
    13801 caagatttat accaattcga agacataaaa tactttttaa aagataataa cgtttatttt
    13861 tggtattaat tttgaacaac attacacatg aagatatttt tttgtagcat tttattggct
    13921 atttcattta tttcacaagc tcaaattacg ccagataatc atcgacaatt gaaattgtat
    13981 gaagacacca ttcgtatgta tggcgatacc ttaatcaata gcaaactgaa agacaacaga
    14041 acgattgcta gttatagaat aattaaaacc ttgacgaagg ctttacgtat cgaaaattct
    14101 tattattaca gctgggattc tttattgcca tggaaaatta tacaagcgga agatggtgca
    14161 tttaaaatat ttacatggta tgctcaaagt gatgaaggta tgtacaaatt ttttggaaca
    14221 ttacaaatga aaagcgacac gctgaaaatg tttccgctca tcgattactc agaatttact
    14281 gaccaccagc aagacattaa cgtcgataac aacaattgga ttggcgcgct ttattatggt
    14341 attaaaacag tgaaaagtgg aaagaaaaac tactatactt tatttggctg ggatggcaat
    14401 aacatgatga gcaacaaaaa agtaatggaa attctttctt tcaataagca gggacaacct
    14461 cgttttggcg cacccattat cgacatggga aatggaaaaa ttttaaacag atttattctt
    14521 gaatttgcaa aagaagcgtc tgcttcattg aattataatg ctactgaaaa aagaataatc
    14581 tatgaccaca ttgcaccgaa tggcaattct ttagaaggat tttttgccaa ctatgttcct
    14641 gatggaactt acgaaggatt tgtatggaaa aaaggaaaat ggcgacacgt agaaaatatc
    14701 gaatacgaaa aacgaacaga cggtgatgta ccaaacgttg gaaaagtaag agaatttgag
    14761 ttgtatcagc cgaaaaaaag tggcaagtag tttacacgag tttatctaaa attaaggcag
    14821 ttgcaatgcc tgcatttaat gactctgctt ttccattttt aggaatggtg atgggaaaag
    14881 tgattttagc ttgcagcgat tcgcgtatgc catgtccttc attgccaata agtataaatc
    14941 ctttttgttc gtaagtttgt actctataat tttcaccatt caacactgcg ccataaactg
    15001 gtaaatcatg ctccataaat agttgctcaa tgtctgtttc tattacgttt actcttccga
    15061 cagacgccat gctcgcttgc actacttttg gattataggc atcaacacaa ccatcagagc
    15121 aaaaaatttg tttaatacca aaccaatcac aagtacgaat aatagtgccc atatttcctg
    15181 ggtcttgaat gtcatctaac accagcgacc attcatttga gttaatactt acatcgataa
    15241 ttggaatttt aaaaacggca aaactattgt gatgatgttg caatgcagta attttattca
    15301 aatctgattc actaataatt cgacattttt gatgaataaa gttgtcgttt attgcaaata
    15361 tgtgaacaac ttccatacct tcatctatta attcttttat aattttatca ccttcggcaa
    15421 taaattctaa atacttctta cggtatttct taattttgag cgaattaata tgttttattt
    15481 cattttggct aagcatgatg caaaattcat ttcaaagatg taaaaaatta ggatatgttg
    15541 tgtgcattgc attactatat tcttgcagtg caaccaaaca tttaataaaa gacgaccaga
    15601 gcttattgat aagcaatggc gtttctataa aatatagcaa caaagtagtc agcgataaaa
    15661 cttcgcttaa atcagaatta gcaaagcaag tagtatataa ccagcagcct aacaaacgat
    15721 ttataagttt gttccgatta aagttaggtg tttatacaat gaatatttta cgaaatgaaa
    15781 agaaagaagc acgtaaaaaa gaattggaaa caaaaattgc caataatact catactgcga
    15841 aagacttatc aaagctgaaa aaactagaga aaaaaaggag aaaaaaaata agtgcaaata
    15901 aaacaaataa tggcgaagcg ccagtattgt ttgactcaac aacaattgca acttctataa
    15961 aaagaatgaa gaattattta ttctatcatg gattcttcta cagtgatgtc acttcatctt
    16021 tcaaaactaa aaacaaaaaa acaagtgtta cctatcacat aaatactgga ccacaattta
    16081 cttatcgaaa aatacatcta acagcattag acagcattct attaaaaata gtagaaaaag
    16141 aagaaaagaa aagcttgctt aaaaaaaatg atccttttga tatcgacatc ataaaaaaag
    16201 aaagacagcg aatagcagaa accatacaaa ataaaggcta tttcaccttc agtcctgatt
    16261 atatttttct aaatattgac agcaccatag gcaatcatca aatcgatgtc aatttaattg
    16321 tcaataatga agcggattcg cttacgcata agctatactc gtatgttcaa atcgattacg
    16381 atattttaaa ttttgacaat aagaaagaaa ataaaaaact aacaagaaaa gattttattt
    16441 acgataccat ttgtgatgtc aactatcgtg ttttgaaaac atcagtactg ccacgagcgc
    16501 tcacaaaatc tacttacata aaacctggcg atttattcaa caaagaccaa ctccttaaaa
    16561 caagaaatgc actaaacagc ttaggtgtgt tccgtttcgt aaatatcgaa cacattccga
    16621 tatctatttc accagaagaa agtggattat acacacgcat caaatgtaca ccagccaagc
    16681 gccatgcatt tggaactgac gtggaattaa acactaatgc gcaaagcaca ctcggtttta
    16741 atcttgtagg gtcatataga aataataacc tttttagatc agcagctaaa ttccaattca
    16801 atgtgagtac aggtttagag tttcaactct taaaagaaaa aagactagaa aataaagcag
    16861 taaacacagt aaatttcaac atggaagcca agctaaactt agcacgtata tttccatctt
    16921 tcaaaaaaaa taaatgtgtt acctatcaaa aatacaaacc gcgtacattc atatcattaa
    16981 actataattt ccaacgtaga ataggattat ataacattca aacggtcggc accaattatg
    17041 gttatgaatg gtataacgaa aaatacagac acatcttttc gcccttgagt tttacttatg
    17101 tcattccatc tcgcatcagc gactcttttc aaacagaatt aaataaaaac gcacgtttac
    17161 aacaaagctt tagtcaacag tttatattag gtcaagaata tacattctct tatacaaatc
    17221 aagacttaaa tgttggaaag tataaaaatt atttctattt cagagccaat gcatttttgg
    17281 ccggcaactt cttatttgca ttttcaaaag cagcacaaaa aaacgcggtg acgccttata
    17341 aattaggcaa agtcaacttt gcgcaatttc tccgtttaga aatggaacca cgctatttct
    17401 tcaatttcag acgtggtcaa gccttgagct tccgcacctt cataggcatc ggcattcctt
    17461 atggcaactc aagatataca acatctgtag ccatcaacga ttccactttt acgcaacgtg
    17521 atgttgctgt aattccatac ataaaacaat ttttcgctgg tggtccaaac tccttacgag
    17581 gctggggtta tcgtcaatta ggacctggtg gctttaatac gcataataaa aacagaaata
    17641 atctcgacca aactggcgat ttaaaatttg aattcaatgc agaatatcgt ttcaatattt
    17701 acaagatttt taaaggcgct atatttacag acatcggaaa cgtttggcta ataaaagaag
    17761 atccagacaa agctctcgcc aacttcgata ctaaacgatt tatgaaagaa ctcgcttggg
    17821 acattggtat tggtttacgg atggacttaa actttttcgt cgtacgatta gacctcggat
    17881 atgctttgta tgatccagca tttaatgaag gtgacagatg gacttttaat aaaataaaca
    17941 atgatgatta tctcattttc aaacaaccac gcaataatga tataggattt tataaattta
    18001 gttttaaaga tttcttagga attaattttg caatcggcta tcctttttaa tacttcagta
    18061 tacgccgtta ttgaatgcga ataagtatga gaaatgccat tatagactac aatagataca
    18121 tacaacagac ttgtatatta ctgtctttgt attttatgat tcaggcaaat agcatcttag
    18181 cgcaacaata ttttcgattt gattatttgc ccgtaaaatc aaacaataca agcttgcaat
    18241 acccttggac tggcggcata aataatgcgc aattcggtat ggttgatgtc aatcacgaca
    18301 atagaaaaga ccttgttgtt tataataaat caaacaataa ttttttgatt tttacttcga
    18361 cacaaaacaa tagcacacaa tatcattttt catcaaaata tgctgctcat tttcctcctg
    18421 ttaatggctg gtttattatg aaagattata actgcgatgg cattgaagat attttcacat
    18481 ataacaatgt ggctaacata aaagtatata ctgcttacta tgcaaatgac actttaaaat
    18541 tcaaattaca acaagatgga tttttttatc agggtgtaag tggcaaaata aacctatatt
    18601 gctcagacgt aataaaacca gctcttgctg atgtcaataa agatggcgac ctcgacgtaa
    18661 tttcatttaa tgtctttggc aatagattgc aatattatga aaatcaacaa aaagaattag
    18721 cgctgccatg tgatagtttg ttttttacaa aagccgataa ttgctgggga aatgtactcg
    18781 acacactagc tgctgcttac gcattaaatg atacatgcac gttcaaattc aatcgaccaa
    18841 acagcaatga aaatattatg cacacaggct cttccttagc agcaattgac attgacgcta
    18901 atgaaaccac tgatcttgca attggaagta ttggattaaa tagcctaacg cttttatata
    18961 acttaggcac taaaaactat gcaagcatac aacaacaaga catcactttt cctaactata
    19021 atattccatt tgatgtaagc tcatttggct cgccttcatt tgttgatgca aacaatgatg
    19081 gtttcaccga tatgttggtt tccacatttg acgttggtac tgccaatatc aataatatat
    19141 ggtactacaa aaacacaaag acaagcggca cgccaagtat tgaactagca ttacaacaaa
    19201 aaaatttcct attggaaaat atgatagatg ctggcgaaaa ttcgtatcca tgtttttttg
    19261 atgctgatgg tgatggctta aaggacatat tgcttggtag tggcggtttt aaagactatg
    19321 tgcaagcagc tgtttataag ctacaattat ataaaaatat tggtaatgcg gagcaagctg
    19381 cattcgaatt aatagatgac gactatctcg gagtaagcac attaaatgta aaagacctcg
    19441 ctcctgctat tggtgatata gataacgaca atgacacaga tatagtcgtt ggactttcag
    19501 acggcaaact tatttactgg gaaaacacag caattactgg caatgcacca aatcttttat
    19561 acaaaggact tttaaaagat gctactggaa ttgcaattaa tgttggtgca aatgcaacac
    19621 cttgcttggt agatatcaat agagatggaa aaacagattt aattattggt gaaagaaatg
    19681 gcaacttaaa ttattatact ggcaattcaa ttgacagtat tgattttgcg tttgtaacag
    19741 attctttagg caaaattaga attaaaacaa atggactttc tatcggcttt acacaaccta
    19801 caatagccga cattaataac gatagtaaat acgatttgct cttaggtacc aatattagtg
    19861 gactgttaaa gtattacaac attgaagata atttaacagg cacttttatt tcatctggaa
    19921 atgtcgtcag cgaaagtata ggtatgcgta cttcttcaac aattgcagat atttctaatg
    19981 acacaaaagc tgaattgtta tgtggtaata ttgctggcgg tttaactatt ttcagtcaat
    20041 ttccaccgcc aattatacca tcgacaattt atgaaaaaag actcgaagaa atcgcatttt
    20101 ctgtttttcc caatccagct aatcacagta tttctgtatc gctaaaggca catttcagta
    20161 atatatctgt tcaaataata aacacgattg gacaggaaat atttcatcaa acatattcaa
    20221 atatcaatac ctttgaaata aacacaagca cctttaacaa gggtctatat ttacttaaaa
    20281 taaacgatgg gaaaaatata ggttttcaaa aaatccttat tcaacattaa caaaaaaagc
    20341 gaaactatcg tttcgctttt tgaggtcgat ggcggattcg aaccgccgta cgagcttttg
    20401 cagagctctg cctagc
//
LOCUS       DAOQQV010000036        20256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_792_length_20256_cov_7.322855, whole genome shotgun sequence.
ACCESSION   DAOQQV010000036 DAOQQV010000000
VERSION     DAOQQV010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20256
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_792_length_20256_cov_7.322855"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(603..2408)
                     /locus_tag="PLL59_05525"
     CDS             complement(603..2408)
                     /locus_tag="PLL59_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY23299.1"
                     /translation="MAAIKSKLIGGNFLFSETDIKDVFIPEEFTEEQKMIWQTVYDFC
                     MQEIHGMGFEKIALMDAEKDKDKVYEIFKKAGELGLYGISIPEEFGGMGADFNTGLLY
                     TEALSLGLSFATTVGAQVSIGSLPIVFYGTQAQKEKYLPKIATGEYGCSYALTEPSSG
                     SDANSAKTKATLSADGTHYLLNGQKMWITNGGFADIFIVFAKIEDDERLSAFIVEKSF
                     GGIEIGKEEKKMGIKSSSTVQLFFNNVPIPKENLLGERGKGFNMALNILNNGRIKICA
                     GGVGGMKFGITKSVEYANQRIQFDQPIAQFGAMKYKLGQMAMLAFINESAVYRVGADV
                     DKKYKEFLANGATENDAKINSMREYAIECAILKVQGSEAICWTADEAIQIHGGMGYSM
                     ETGVEMAYRDARITKIYEGTNEINRMLALAEFYKRAFQTKELKINDAAKSIPLGIAQN
                     FNPFNNGFLADESEIVSNLKSVFMIITGAAGRKLKTKLVDEQEIVMNLADIMSVAFMA
                     ESAILRVKKLNADKNTDKDQLKIKIKMTQLYVYEALEIARKAADNAIDSYASGVEKFT
                     MKRVVGSLLKNYDINPKNTRRAIADAVIEANKYPF"
     gene            2597..4846
                     /locus_tag="PLL59_05530"
     CDS             2597..4846
                     /locus_tag="PLL59_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23300.1"
                     /translation="MKRNKMSYDDKTMLLFRKIALCILMHLFFNVVNANDSININQTI
                     LSTSYPNKFTGVSSMSMYELVKSNYDSSAQTYQTAVYNNIYDLLKPLKLKVLRFPGGT
                     IGNYYHFYGRGHGIDTSETTCAPGRVGNATFANMHVAFDNRVNKNIIEYLTEELDTLE
                     KYGPPIDIAFRINSHTHFYKYDLRKYTDSIQYLIYQYFAGDTAFLSTDGSSMDSVKIN
                     AIATKLMQLQQNTLYTRIKQQLARDTGFARRFKENMDAVAYLKGKGVNIIGAEIGNET
                     NAEYVVFDDDFDYLGFDCTQMPANFNFNILSLPMKYYIEGMIKNNLIVSLYADSLKAK
                     YNIVSGVPAATSHNYLTLNANYEPMHIKPYDLGAKKSDLWNKYYASQQQVFALIPHLY
                     SQEFIDCADFLGADTIYGLPEKRIHKIAERFYKFFIDSLITYNLKRVNYYGNNKPQWI
                     TEWNFSDGSYANNTFLHALYNYYFIRKAIEIHDFSPNYVQILLYHHLVGASQNWPLIK
                     VYNTGQSLITQQQITYHPFYIWSNTIAQQVKKVKTPFWATTDNQITDVFINNIKNELI
                     IQFVNTDSVAHFIDLKKISITDNNTELIAQKIDRYCLDAENFISTNGSYCAFFTNPMY
                     QKAYQVFDDSLQQLDTLWMPAISMGKFTLHLKNKVTTAVQNEKKNTVFRLFPNPAKEE
                     ICVQHSNTSAKANYQYMIYDSLGKLVANGIITQQNTTINIEKFARGNYVINVINNNEF
                     IDSLKFIKY"
     gene            4927..5466
                     /locus_tag="PLL59_05535"
     CDS             4927..5466
                     /locus_tag="PLL59_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HMY23301.1"
                     /translation="MKKLTIIFLFTITLFSQQKSYAAGGFCSMLDKICMQKKIDADAF
                     SNFFQKININISKASDLGMYANIFKWLYTPYRYGGKTERGIDCSNYVFKLMDDCSDTY
                     ATSKQLADITDYVDREELKEGDLIFFNMKGYGISHVGVYLQDGKFTHSSSSKGVTISS
                     LDEPYWNTRYCRAGRINSK"
     gene            complement(5512..5940)
                     /locus_tag="PLL59_05540"
     CDS             complement(5512..5940)
                     /locus_tag="PLL59_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HMY23302.1"
                     /translation="MIALSVVRQFEILLNAYPRGFHLITAEILQSISTFPQTGICHIF
                     IQHTSAGIMINENADVDVRIDFEAWFDRAVKENEPYYQHTIEGSDDMPAYIKSSLVGH
                     CITIPIKNHRLYLGTWQGIFLCEFRNKASRRKLIITLIGE"
     gene            6016..7212
                     /locus_tag="PLL59_05545"
     CDS             6016..7212
                     /locus_tag="PLL59_05545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMY23303.1"
                     /translation="MLPTSDTLNRVSESATIKMAQLSRELAAKGVNVISLSLGEPDFN
                     TPKHIKEAAKQAIDSGKYDSYSPIAGYLDLKQAICEKLKRDNNLEYKPENIVVSTGAK
                     QSLANVFISFLNEGDEVIIPAPFWVSYPEQVKLCNATCVIVPTTVDADFKMTAEQLEK
                     AITPKTKFLVYSSPSNPTGAVYTRDELAAFVQVLEKHPHVHIISDEIYEHINYAGKHV
                     SIAEFPSMKDRTIVVNGQAKGYAMTGWRIGYIAAPAYIAKACDKLQGQITSGTNSVAQ
                     HATITALLGDQAPTEAFRQAFQKRRDLVLGILNEIPNVKCNIPTGAFYVFPDLSAYFG
                     KVFEGETIKDADDLCLYLLNVGHVAAVSGSGFGAPECIRFSYAASEENLIEAFTRLKN
                     ALAKLA"
     gene            7287..8249
                     /locus_tag="PLL59_05550"
     CDS             7287..8249
                     /locus_tag="PLL59_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMY23304.1"
                     /translation="MSLDFDISGFKHKRILVVGDIMLDEFIHTKHYRNSPEYKDVPIF
                     NILQKKSYLGGAANVALNIKKLGATPYLIGITGIDDASKTIQSLLKENDISNAYLYEN
                     HKINTTKKTRLFRDGKPICRIDEDANNSNDTLCNQFILRNLQQAIIQHKPHAIILQDY
                     NKGVLNADTIPAIIALAKQHHLLIAVDPKFDNWNLYKEVDLLKPNLEEFCFMCDSKKE
                     HFNLERNAKILQEIIKFKILLLTLGADGNFIFDGQYFQTTAIDTPIQNADVCGAGDTV
                     IAVATLALLCNFSLTQTATLSNKAGLIICQKNQVQAISFDELSQ"
     gene            complement(8262..9524)
                     /locus_tag="PLL59_05555"
     CDS             complement(8262..9524)
                     /locus_tag="PLL59_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMY23305.1"
                     /translation="MHKVLRVLGETLKLTFQQLWLNKLRTFLSLFGITVGIFSIIAIL
                     TAVDALKNNINQSINSLGDNILFVNKWAWTFDGPSDYPWWKYFQRPNVNFDNYKFVQS
                     KSKLAEAVTYELNPRVAPEVAYKNKKTHKSVISAVTDNYGKVFQLDIAQGRFLSSMEI
                     NNGINVCVIGGDIADKLFKNIANPVGLEIRMDGKPLTVVGVIAKKGQDLLGFNYDKAV
                     IVPLDYYTRYFTVDDNAVDKTLEIRAKPDVSLAALKNELIGIMRSARKLRPSQLDNFS
                     INQLDVIAKGFDSMFDSLSMVKWVIGLFSLIVGGFGIANIMFVSVSERKALIGIKKAL
                     GAKRTDILLEFLLEAIFLCMMGAIVGLALVYVMCIFATEGSGMEFKLTSINIMIAFGI
                     SFVLGILAGIIPAYLAATMDPVEAIRSK"
     gene            9651..10700
                     /gene="queA"
                     /locus_tag="PLL59_05560"
     CDS             9651..10700
                     /gene="queA"
                     /locus_tag="PLL59_05560"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HMY23306.1"
                     /translation="MKLSQFDFELPKEKIALYPSKQRDECKLMVVHRKSGKIEHKTFK
                     NVLDYFDDGDVMVINNTKVFPARMYGTKEKTGAKIEVFLLRELNHDLRLWDVLVDPAR
                     KIRVGNKLYFGDDDLVAEVVDNTTSRGRTIRFLFDGTDADFRAVLDKLGNTPLPKYIK
                     RDPEDSDKENYQTVFASQEGAVAAPTAGLHFSKELMKRLEIKGVDFAELTLHIGLGTF
                     RSIDVEDLSKHKMDAEYFRIDEKCTKIVNKALQRKKNVCAVGTTSMRSIETAVSAEGL
                     LKPMEGWTNLFIHPPYDFSIANSMITNFHVPKSSLIIMVSAFAGFDLTMEAYQKAMEK
                     KYNFFSYGDAMLILD"
     gene            complement(10838..12124)
                     /gene="purD"
                     /locus_tag="PLL59_05565"
     CDS             complement(10838..12124)
                     /gene="purD"
                     /locus_tag="PLL59_05565"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMY23307.1"
                     /translation="MNILLLGSGGREHAFAHSIAKSNKCSQLFIAPGNPGTLQLGINV
                     PIATTDFAAIKSFALENEIKMIIVGPEAPLVEGIYDFVVNDAELRHIPVIGPSKIGAQ
                     LEGSKAFSKAFMMRHQVPTAAYAEFTMATLSEGLAYLSQQTPPIVLKADGLAAGKGVL
                     ICSSIDEAKDEFKQMLDGKFGDASAKVVIEEFMDGIEFSVFVLTDGKDYKILPTAKDY
                     KRIGEADTGLNTGGMGAVSPPPFVTDDMMKTVEDTIIKPTIQGFQKDGIVYIGFVYVG
                     LMYLKTGEIKVVEYNCRMGDPETEAVFPRWKNDIIEVFQSTVDGRLSNVDIDIDERAA
                     TTIILASGGYPEDFEKGKAITGIDEIKDSIVFHCGTKLIEDDKGQSILVSNGGRVLAV
                     TSLHVDWKSALAISNKNASIIQYEAKYYRKDIGFDL"
     gene            12272..15586
                     /gene="secA"
                     /locus_tag="PLL59_05570"
     CDS             12272..15586
                     /gene="secA"
                     /locus_tag="PLL59_05570"
                     /inference="COORDINATES: protein motif:HMM:NF009536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HMY23308.1"
                     /translation="MLNFLQKGLSKIFGSKSNRDIKSLEPIIAAVNEHFYAYQNISSD
                     ELRQKTQDFKFRIQGYLSAIDKEINTLTEKAEQDNLDLDEKEAIFSKVDKLKKERDEA
                     IEEILQDIMPEAFAVVKEASRRFSTNETIRVKATALDRELAVKNPHIKIEGDDAVFPN
                     KWSAAGGEISWNMVHYDVQLIGGAVLHKGKIAEMATGEGKTLVSTLPAYLNALAGCGV
                     HIVTVNDYLARRDSEWNGMLFNFLGLTVDCIDKHEPHTDGRKNAYLADITYGTNNEFG
                     FDYLRDNMVRSAEEQVQRKLHFAMVDEVDSVLIDDARTPLIISGPVPKGEEQMFQELK
                     PRIERLVSTQKKAVQQFLNDAKRLIGEGNVGVRNGEGGLALLRAHRGLPKNTALIKFL
                     SEQGNKQILMKTENHYLQDQQKEMPTCDAELYFVIDEKNNQIDLTDKGIELITSSGEE
                     KDFFVMPDVGALIAEIENEPVSYEEKLKRKDEVLLDFNIKSERLHTVNQLLKAYCLFD
                     KDVEYVIIDGKVKIVDEQTGRIMEGRRYSDGLHQAIEAKENVTIEAATQTFATITLQN
                     FFRMYHKLCGMTGTAETEAAEFWEIYKLDTVVVPTNRPTQRDDKEDLIFKTKREKYNA
                     IIEEINKLREEGRPVLVGTTNVEVSELLSRMLSMRKIPHNVLNAKQHQREAEIVAQAG
                     LSGQITIATNMAGRGTDIKLGQGIKDIGGLAIIGSERHDSRRVDRQLRGRAGRQGDTG
                     SSQFYSSLEDNLMRLFGSDRIAGMMDKMGYKEGEVIQHSLVTKSIERAQKKVEENHFG
                     TRKRLLEYDDVMNAQREVIYKRRQHALIGERITFDLINMFYDLSAEICNDAKLSQDIE
                     AFKLDCIRFLSVDTSITAEDLKNKTEEDLAAHLYHEVFEKYTRKSENIADEAYPIIKN
                     VYETKGNQYQNIVIPFTDGNRNINIIANLEKSYNNKGRELIKVFEQNIILSFIDHHWK
                     DHLRQMDELKHSVQMAVHEQKDPLLIFKFEAYELFKQMLSAVNKDVTTFLMKGSLPVQ
                     DANQIQQHRQLRRAERTTETRGDGTTEEEYAGAETAETKEKQQPARAEVKIGRNDPCP
                     CGSGKKFKQCHGKEMA"
     gene            15629..16063
                     /locus_tag="PLL59_05575"
     CDS             15629..16063
                     /locus_tag="PLL59_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMY23309.1"
                     /translation="MKNVLSILCVCVASTCFAADSDSTAIDIKNNILFFKDPRVDVLQ
                     KMYMRKAVDPNAKVIRVQVFQAIARDKVFEAKAQFSARYPGIQTFVTYASPNFRLRVG
                     EFSSQQEAFKFMQQVKPHFPMSFVIEEKGSKNDDKPKSKSGN"
     gene            complement(16060..18303)
                     /locus_tag="PLL59_05580"
     CDS             complement(16060..18303)
                     /locus_tag="PLL59_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMY23310.1"
                     /translation="MNNVLNWLKTPKNLWKLFAIMVGSVVLFFTLVRIGLFGALPSFE
                     ELENPSANLSSEIYSADSVLIGKYYVDNRSNISYEELSPFLVQALIANEDERFYEHSG
                     IDLKRTLAAVFFAGQHGGGSTVTQQLAKNMFHKRAQNIVQRVLQKAKEYVIAVMLERR
                     YTKEEIIAMYFNTFDFVNNAVGIESASRIYFNKKPKDLKVEEAAMFVGMLQNPSYYNP
                     RRNEVFAKRTQERRATVLRKMMEKGNITTAQFDALKDKPIELDFHPESANDGLAPYFR
                     MVVAEELKKWAKENKNLNPDGREYNIYTDGLKIYTTINTRMQRYAEEAAVQHLARYQG
                     IFNSQFGGSWKPWDTKAGKTILETACKYSYRWAALKERGWSNDAILANFKTDKHRMKV
                     FTYKGMKDTTLTPYDSVKYYKSFLQIGMMVMEPYTGYVRAWVGGPNFQFFKNDHCRPS
                     TKRQVGSTFKPIVYGLAVDNGWSPCMVIAPGMVRIGNWAPRGGSGGPQTLLQALKTSN
                     NQMAAYIIKQFGVNPVIDLSRRMGIASDLPPYSPLALGAADISLFEMMNVYSTFPSGG
                     ISVKPQYLLRIEDKDGNVIQNFTTEMKEVFSDNTAYKMCQLMKGPVSRGGTGASLPAY
                     FSYANVDVAGKTGTTNKNTDAWFIGFTPDLLAGVWVGCDDPLLHFLYTSSGQGGRAAM
                     PVFGMFMQKAYGDPKLALNKSAKFFRPSDSTLVKDLCEESGQSIINDGSTGNWGQVDE
                     DVSDSEY"
     gene            complement(18300..>20256)
                     /locus_tag="PLL59_05585"
     CDS             complement(18300..>20256)
                     /locus_tag="PLL59_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY23311.1"
                     /translation="YYEYLLAQLYQLNNQDADAADYFVKAGKHTKSEELYLFSKIQLA
                     KLYSDNPGLANQDIVKMLSKLIKFGKNKNHADEVLYTIANYYYYQKDTANAVAYLEKS
                     IKKSLDNKEQKGKSYLRLGEIYFDKEAYDKAAIFYDSAIAYLPNTFENYQQIVTRKEV
                     LGDLATYAQTIYTEDSLQRLGRMSPKELDKFLAEVKAQKEKADKKKSRFSSDIGDGNT
                     SAASAFVPETGVSNGLWYFYNPESKSKGYNDFLRIWGDRKLEDNWRRSVKSGFLEDII
                     STDETAINDTVTAAVKNKNIPKATTTTTDTLKIPKTPEDFLRSDIKIANALYGTGEIF
                     KNKLNNIPKSKQAFDELIRRFPNSEFDAIAHYYEYLIYKEQNLNGLAQKEKDYILQNY
                     PMTEVAEVLNNEGKIRVVDESIETAEKLYASTYQLFLNDKFDDVIKNRFVAKTKYPNA
                     PQQAQFEMLEALSYGKIKQFQAYKKALSDIILKYNDGDVKKKAQEYLIAYIQHENKLN
                     DTSTQAIQNSNTPKTLDTISSKFVMDTTDLFVLVQLKDKYITVSDVVSSIHKFNKKKF
                     DYLKIKINPFFIESYAILQMKKFEGLAEALQYYNTITENVSEAVGATNVQKSSFYIIA
                     PSNFKLIKTIADLDIYNTFFRTNYLNK"
BASE COUNT         6683 a         3644 c         3426 g         6503 t
ORIGIN      
        1 gctgacctca taaacacgtt cgggtatcat tccccaataa gaaagtgcgg attctagcga
       61 aacataactg ggacccctca agtggttagc aataagcagt ggatgtggca atggtaaatc
      121 ggtttcagga ccaggcacat acaatcctct ccgtagcgac agcagttctc cgctcctgat
      181 caactcactg attttatcgt tcggacgatt gtattcagtg agcatttcct ccacaacatg
      241 atgtgatatc ggtgcttccg catactcttt tacccttctc ctgaatgaca caatgcaatt
      301 ttacaacaca ataatcggaa aaattccgat tattacaaaa tgtttttaca taaaattgca
      361 aaataaaatt agcttaatcg gaatttttcc gattaaatac cattttctat ctcaaaaagt
      421 accatttttg accactttac ttttttaaat ataaaaagag gctgtctcaa aacaaaaaga
      481 agagaacact aagattataa tattctgaat tttatcagat acatatccta atagaaaaga
      541 ggctatctca actattgaga cagcctcttt tcatttcgtc aaatgtctat atcaaaaaga
      601 tattaaaacg gatatttatt cgcttcaatg acagcatctg caatagctct acgtgtattt
      661 tttggattga tatcgtagtt tttcaataaa gaaccaacta cgcgtttcat cgtaaatttc
      721 tctacacctg aagcataact atcaattgca ttgtcagccg ctttacgagc aatttctaat
      781 gcttcatata catacaattg cgtcattttg attttgattt tcaattggtc tttatctgtg
      841 tttttatccg catttaattt tttaacacgt aaaattgctg actcagccat gaatgctact
      901 gacatgatat ctgccaagtt catcacgatt tcttgttcgt ccactaattt cgttttcaat
      961 ttacggccag ctgcacctgt gataatcatg aatacgcttt tcaagttgga aacaatttca
     1021 gactcatcag ctaagaagcc attattgaaa ggattgaaat tttgagcaat gcctaatgga
     1081 atagattttg cagcatcgtt tatttttaat tctttcgttt ggaaagcacg tttatagaat
     1141 tctgctaaag ccaacattct attgatttca ttagtacctt catatatttt agtaatacgc
     1201 gcatcgcggt aagccatttc tacgcctgtt tccatagagt aacccatacc gccatgaatt
     1261 tggatagctt cgtcggccgt ccagcagatg gcttcagaac cttgtacttt taaaatagca
     1321 cattcgatag catactctcg catagagttt attttcgcat cattttctgt agcaccattt
     1381 gctaaaaact ctttatattt tttgtcaaca tcagcaccta ctctatacac tgctgattcg
     1441 ttgatgaatg ctaacattgc catttggcct aacttatatt tcatcgcacc aaattgtgcg
     1501 attggttggt caaactgaat gcgttgattt gcatattcta cagatttagt aatgccaaac
     1561 ttcataccac caacaccacc agcacatatt ttaatacgac cattatttaa gatgtttaat
     1621 gccatattaa aacctttacc acgctcgcct aataagtttt ctttaggaat tggaacatta
     1681 ttaaagaaca attgaacagt agatgaagac ttgataccca tctttttctc ttctttaccg
     1741 atttcaatgc cgccaaacga tttttctacg atgaaagcag ataaacgctc atcgtcttca
     1801 atttttgcaa aaacaataaa gatatcagca aaaccaccgt tagtgatcca cattttttgg
     1861 ccgttcaata aatagtgtgt accatctgca cttaatgtcg cttttgtttt tgctgagtta
     1921 gcatctgaac cagagcttgg ctctgttaat gcatatgaac agccatattc acctgttgca
     1981 atttttggta aatatttttc tttttgtgct tgcgtgccat aaaatacaat tggcaatgaa
     2041 ccaatcgaaa cttgcgcacc taccgttgtt gcaaacgaca agccaagtga taaagcttct
     2101 gtatataata aacctgtatt aaaatcagcg cccattccac caaactcttc tggaattgaa
     2161 ataccgtaca agcctaattc accagctttt ttgaatattt catacacttt atctttatcc
     2221 ttctcggcat ccatcaatgc tattttttca aagcccattc catggatttc ttgcatacaa
     2281 aagtcgtaca cagtttgcca tatcattttt tgctcttccg tgaattcctc tggaatgaaa
     2341 acatctttta tatcggtttc tgaaaaaaga aaatttccac cgattaattt actttttatt
     2401 gctgccataa taattacatt tttttatcga actaaagtta attcaaaaaa tccaatgcat
     2461 gcattggatt aataataatt taacgcctta ttgcaaataa ttaatctatg ttaattgaaa
     2521 ctaagggaaa aaatagagtt taaataaaaa tgtatataaa taaacattgg aaattaaaga
     2581 atgagtatat ttgttcatga aaagaaataa aatgtcgtac gatgacaaaa cgatgctatt
     2641 gtttaggaaa atagcattgt gtattttaat gcatctcttt ttcaatgttg tgaatgctaa
     2701 tgacagcatt aatatcaatc agacaatttt gagcacaagc tatcctaata aatttactgg
     2761 tgttagcagt atgtcaatgt atgaacttgt caaaagtaat tatgatagta gcgcacaaac
     2821 ttatcagaca gcagtttaca ataatattta tgatctatta aaaccattaa aattaaaagt
     2881 attgcgattc cctggtggaa caattggaaa ttactatcac ttttatggac gtggtcatgg
     2941 tatcgacaca tcagagacta catgtgcgcc aggccgagta ggcaatgcca catttgccaa
     3001 tatgcatgta gcatttgaca atagagtcaa caaaaacatt attgaatatt tgacagaaga
     3061 attagatacg ctagaaaaat atggaccacc aatagacatt gcatttcgaa tcaactcgca
     3121 cactcatttt tacaaatacg atttacgtaa atacactgac agcattcaat atttgatata
     3181 tcaatatttt gctggagaca ctgcatttct tagcactgat ggcagtagta tggacagtgt
     3241 aaaaataaat gcaattgcaa caaaattaat gcaattacaa caaaatactt tatacacacg
     3301 cattaaacag caattagcta gagatactgg ttttgctcgc agattcaaag aaaatatgga
     3361 tgcagttgcc tacttaaaag gcaaaggtgt aaacattatc ggtgctgaaa ttggaaatga
     3421 aacaaatgcg gaatatgtag tatttgatga tgatttcgat tatttaggat ttgactgcac
     3481 gcagatgccc gcgaatttta atttcaatat tttatcactt ccgatgaaat attatatcga
     3541 aggaatgata aaaaacaatc ttatagtttc attatatgct gatagtttaa aagcgaagta
     3601 taatattgta tcaggtgttc cagcagcaac gagtcataat tatttgactt taaatgccaa
     3661 ctacgaacct atgcatataa aaccgtatga tctaggtgca aaaaaatcgg atttgtggaa
     3721 taaatattat gcatcacaac agcaagtgtt cgccttaatt ccgcatttat atagtcagga
     3781 atttatagac tgtgctgatt ttttaggtgc agatactatt tatggattgc ctgaaaaacg
     3841 catacataaa attgctgaac gcttttataa atttttcatc gattctttaa ttacatacaa
     3901 tctaaagcgc gtaaattatt atggaaataa taagccgcaa tggattacag aatggaattt
     3961 ttctgatggt agttatgcca ataatacatt tctacatgcg ctttataatt actattttat
     4021 tcggaaagcc atagagatac atgatttttc gcccaattat gttcaaatat tattatacca
     4081 tcatttagtt ggtgctagcc aaaattggcc tttgataaaa gtgtataaca ctggccaaag
     4141 tttaatcacg cagcaacaaa ttacctatca tccattctat atttggagta atacaattgc
     4201 gcaacaagta aaaaaagtta aaacaccatt ttgggctacg acagacaatc agattacaga
     4261 tgtgtttata aacaatataa aaaatgagtt gattatacaa tttgtaaata cagattctgt
     4321 agcacatttt atcgacttaa aaaaaataag catcacggac aataacacgg aattaattgc
     4381 acaaaaaata gatagatatt gtttagatgc ggaaaatttt atcagcacta acggaagcta
     4441 ttgtgcattt tttaccaatc caatgtatca gaaagcttat caggtattcg atgattcatt
     4501 gcagcaactt gacacattat ggatgcctgc tattagcatg ggaaaattta ctttacatct
     4561 gaaaaacaaa gtgacaacag cagttcaaaa cgagaaaaaa aacactgtat tccgtctatt
     4621 tccaaatccg gcgaaagaag aaatttgtgt gcagcatagc aacacttcag caaaagctaa
     4681 ttatcaatat atgatatatg atagtctcgg aaaattagtg gcaaatggca ttattacaca
     4741 gcaaaatact acgataaata tcgaaaaatt tgcaagaggt aactatgtta ttaatgtcat
     4801 aaataacaac gaattcattg attctctgaa gtttataaag tattaatttc tttttaacaa
     4861 gtttttaacc gtgtcacatt gctaaaaatt tgtctcacta ctaattggta tgtatttttg
     4921 tcttagatga aaaaactaac catcatattt ctttttacta tcacactgtt tagccagcaa
     4981 aagagctatg ccgcaggtgg tttttgcagc atgttggata aaatatgtat gcaaaagaaa
     5041 atagatgccg atgcgttcag taattttttt caaaaaataa acatcaatat ttctaaagcg
     5101 tccgacttag gtatgtatgc taatattttc aaatggctat acacacctta tcgttatggt
     5161 ggaaaaacag aaagaggaat tgactgctct aattacgtat ttaagttgat ggatgactgc
     5221 tccgacactt acgcaacgtc taagcaatta gcagatatta ccgattatgt tgaccgtgaa
     5281 gaattgaaag aaggtgattt aatatttttc aatatgaaag gttacggcat ttcacatgtt
     5341 ggcgtttact tacaagatgg aaaatttacg cattcctctt cctctaaagg tgttaccatc
     5401 agctcgcttg acgaacccta ttggaacacg cgctactgta gagctggcag aataaattca
     5461 aaataattta tcaaaaataa tcataaaaaa ggtgctcgac gagcaccttt tttattcgcc
     5521 tataagtgtt attattaatt ttcttcgtga agctttattt ctaaattcgc acagaaaaat
     5581 accttgccat gtgcctaaat aaagcctgtg attttttata ggaatagtaa tacaatgtcc
     5641 tactaaacta gattttatat atgctggcat atcatcacta ccttcaatag tgtgttggta
     5701 atatggttca ttttctttta ctgctctatc aaaccaagct tcaaaatcta tacgcacatc
     5761 aacatcagca ttttcattaa tcataattcc agccgatgta tgttgtatga aaatatgaca
     5821 aatgcctgtt tgcggaaatg tagagataga ttgtaaaatt tccgcagtaa tcaagtgaaa
     5881 acctcttggg taagcattta gcagtatttc aaactgacga accacactta aagctatcat
     5941 tttattttaa cataatatct gaaaagatgc cataaatttc gaaaaccata catttgtatt
     6001 ttaaaattaa gtattatgtt accaacttca gacacgctca atagagtatc agaatctgca
     6061 acaataaaaa tggcacaact cagcagagag ttggcagcga aaggtgtaaa tgtcatttca
     6121 ctttcgttag gtgaacctga ttttaataca ccaaaacaca ttaaagaagc agcaaaacaa
     6181 gccatcgaca gtggaaagta tgactcgtat tctccaattg ctggttattt agatttaaag
     6241 caagctattt gcgaaaaatt aaaacgcgac aataatctag agtacaagcc agaaaatatt
     6301 gttgtttcta ctggcgctaa acaaagctta gcaaatgttt ttatttcctt tttaaatgaa
     6361 ggtgatgaag tgattattcc cgcaccattt tgggtttctt atccagagca agtaaaattg
     6421 tgcaatgcca cttgtgttat cgtgcctact actgtagatg ctgattttaa aatgactgca
     6481 gagcagttag aaaaagcgat aacgccaaaa acaaaattct tagtgtattc gtcgccaagc
     6541 aatccgacag gcgcagtata tacgcgtgat gaattggctg cttttgtaca agtattagaa
     6601 aagcatccac atgttcatat aatttctgac gaaatctatg aacacatcaa ctatgctggc
     6661 aaacatgttt ccattgctga atttccgtcg atgaaagaca gaacaatcgt agtaaacgga
     6721 caagcaaaag gatatgcaat gacaggttgg agaattggat atattgcagc tcctgcttac
     6781 attgctaaag cttgtgacaa attgcaagga caaattacat caggcacaaa ctcagttgca
     6841 caacatgcaa caattacagc acttttgggc gaccaagcac caacagaagc attccgccaa
     6901 gcattccaaa aaagaagaga tttggtttta ggtatcttaa atgaaattcc aaatgtgaaa
     6961 tgcaatattc cgactggcgc cttttatgtt ttcccagatt tgagcgctta ttttggaaaa
     7021 gtattcgaag gagaaaccat aaaagatgct gacgatttat gtttatattt attaaatgtt
     7081 ggtcatgtgg cagcagtaag tggtagcgga tttggtgcac ctgagtgcat acgtttttca
     7141 tatgcagctt cagaagagaa tttaatagaa gcttttacaa ggttaaaaaa tgcattagca
     7201 aaattagcat aagctcactg aattacacat tatatatgac cttcaagttt acactttgaa
     7261 ggtcattttt tttgtacttt tacatcatgt cattagattt tgatatttct ggttttaaac
     7321 acaaacgaat acttgttgta ggtgacatca tgttagatga atttattcat actaagcatt
     7381 atcgaaattc tccagagtac aaagatgtac ctatctttaa tatattacaa aaaaaatctt
     7441 atttaggtgg cgctgccaat gttgcattaa atattaaaaa acttggcgca acaccatatc
     7501 tgataggcat tacaggcatt gatgatgcat cgaaaactat tcaatcttta ttaaaagaaa
     7561 atgatatcag caatgcttat ttatatgaaa accacaaaat aaacacgaca aaaaaaacaa
     7621 gattatttcg tgatggcaaa cctatttgtc gaattgatga agatgcaaat aatagtaatg
     7681 acacgttgtg taatcagttt attttacgaa acttacaaca ggcgatcata cagcacaaac
     7741 cgcatgcaat tattttgcaa gactataata aaggcgtatt gaatgcagat actattcctg
     7801 caataattgc attagcaaag caacatcatt tattaattgc tgtagatcca aaatttgaca
     7861 attggaattt atacaaagaa gtggatttgt taaagcctaa cctagaagag ttttgtttta
     7921 tgtgtgatag taaaaaagag catttcaatt tggagaggaa tgcaaaaatt ttacaagaaa
     7981 taataaagtt taaaatttta ctgctgacac ttggcgcaga tggaaatttt atttttgatg
     8041 gccaatattt tcaaaccaca gctattgaca caccaataca aaacgctgat gtttgtggcg
     8101 ctggcgatac ggtaattgca gtagcaacac ttgcattgtt gtgtaatttt tctttaacac
     8161 aaactgctac attgagtaat aaagctggtc taatcatttg ccagaaaaat caggtgcaag
     8221 caatttcctt tgatgaactt tcgcaataac aatctttgat tttatttgct tctaatggcc
     8281 tccacaggat ccatggtagc agctaaatat gcaggtatga tgccggccaa aataccaagt
     8341 acaaacgata ttccaaaagc aatcatgata tttattgatg ttaatttgaa ctccattcca
     8401 ctaccttctg tagcaaaaat acacatcaca tacaccagtg caagtccaac aatagcgccc
     8461 atcatacaca agaaaatggc ttctaataaa aattccaata aaatatcagt gcgcttggct
     8521 cctaaagctt tcttgatgcc aattaatgcc tttcgttcag aaacacttac aaacataata
     8581 tttgcgattc caaaaccacc aacaatcaac gaaaacagac caattaccca tttgaccata
     8641 cttagtgagt cgaacatact atcaaatcct ttggctatga catctaattg attgatagaa
     8701 aaattatcta gttgagaagg tctaagtttg cgtgcagaac gcataattcc tattagttca
     8761 ttttttaaag ctgcgagcga aacatcaggt tttgctctaa tctccaatgt tttatcaacc
     8821 gcattatcat ctactgtaaa atagcgtgta taataatcga gtggcacaat gacagcttta
     8881 tcataattaa agcccaataa atcttgccct ttttttgcaa ttacgccaac aacggtaagt
     8941 ggcttaccat ccattcgaat ttcaagacca acaggatttg caatattttt aaataattta
     9001 tctgcgatat cgccaccaat aacacacaca ttaatgccat tattaatttc cattgatgat
     9061 aaaaatctac cttgtgcaat atctaactga aaaacttttc cgtaattatc cgtaacagca
     9121 gaaatcacac ttttatgtgt tttcttgttt ttgtatgcta cttctggcgc aacgcgtgga
     9181 ttgagttcat acgtaactgc ttctgctaat tttgattttg attgcacaaa tttataatta
     9241 tcaaaattca cattcggtcg ctggaaatat ttccaccaag gataatcaga tggcccatca
     9301 aaagtccaag cccatttatt gacaaacaaa atattgtcac ctaagctatt tatcgactga
     9361 ttgatattat tttttaatgc atcaacagct gtcaatattg ctataatact aaaaatcccg
     9421 acagtaatgc caaatagaga tagaaaagta cgtagcttat tcagccataa ttgctgaaac
     9481 gtcagcttta aagtttcacc taaaactctg agtactttat gcatacaaca aacctacaaa
     9541 atttaatcaa ataaaaatat ttgtcgtttg taatttggca ttcttacact tacctttgtg
     9601 gtgcataaaa atatgctttc tattattccg taaaataata cttctacata atgaaacttt
     9661 cacaattcga cttcgaacta cctaaggaaa aaatcgcact gtatccttct aaacaacgcg
     9721 atgaatgtaa gcttatggtc gtacaccgca aaagcggaaa aattgagcat aaaacattta
     9781 agaatgtttt agattatttt gatgatggcg acgtcatggt gattaataac accaaagttt
     9841 ttcctgcgcg tatgtatggc acgaaagaaa aaacaggagc taaaatcgaa gtgtttttac
     9901 tcagagaatt aaaccatgat ttacgattat gggacgtttt agttgaccca gctcgtaaaa
     9961 ttcgtgtggg caataaatta tattttggtg atgatgattt agtggctgaa gtggtggaca
    10021 atacaacatc tcgaggcaga acgattcgtt ttttatttga tggtactgat gctgatttca
    10081 gagcagtatt agacaaatta ggcaatacgc cacttccaaa atatattaaa agagatccag
    10141 aagactctga caaagaaaac tatcaaactg tttttgctag tcaggaaggc gcagtggctg
    10201 ctcctactgc tggtttgcat ttcagtaaag agctgatgaa acgtcttgaa attaaaggtg
    10261 ttgattttgc agaattaaca ttacacatag gcttaggtac tttccgttct attgatgtgg
    10321 aagatttatc taagcacaaa atggatgcag aatattttag aattgatgaa aaatgcacta
    10381 aaattgtcaa caaagcatta caacgcaaaa aaaatgtatg cgctgttggt acaacatcga
    10441 tgcgctctat agaaacggct gtttctgctg aaggtttatt aaaaccgatg gaaggttgga
    10501 caaatctatt tatccatccg ccgtatgact ttagtattgc caatagtatg attacaaatt
    10561 tccacgttcc taaatcaagt ttaatcatca tggtatctgc atttgcaggc tttgatttaa
    10621 cgatggaagc ttaccaaaaa gcgatggaga agaaatataa tttcttttct tatggcgatg
    10681 ccatgttgat attggattaa cttcaaaaga aaataaatta aaagaggctg tctcaagatt
    10741 cgagatagcc tcttttttat tcgtaacata tctgagaaaa ttcagaacat ttaaatctta
    10801 gcgttcttct ttttaatgtt gagagagcat cattttatta taaatcaaag ccaatatctt
    10861 ttcgataata cttagcttca tattgtatta ttgaagcatt tttatttgaa atggctaatg
    10921 cagatttcca atctacatgc aaagaagtaa cagccaaaac acgaccaccg ttgctcacta
    10981 aaatactttg acctttgtca tcttctatta atttagtgcc acaatgaaaa acgatagaat
    11041 cctttatttc atcaatacct gtaatagctt tccctttttc aaaatcctct ggatagccac
    11101 cactggctaa aatgatagta gtagctgcgc gttcatcaat atcgatatcc acgtttgaga
    11161 gtctgccgtc aactgtggac tgaaacacct caattatatc atttttccac ctaggaaaaa
    11221 cagcttcagt ttctgggtcg cccattctac aattgtattc cactactttt atctcacctg
    11281 ttttgaggta cattaatcca acatacacaa atccaatata tacaattcca tctttttgaa
    11341 aaccttggat agtcggctta atgatagtat cttcgacagt tttcatcata tcatcagtaa
    11401 caaatggcgg tggcgaaacg gcgcccatgc caccagtatt taagcctgta tctgcttctc
    11461 caattcgctt ataatctttt gctgttggta atattttata atcctttcca tctgttagta
    11521 caaacactga aaactcaata ccatccatga attcttcgat caccaccttc gcacttgcat
    11581 cgccaaattt cccatcgagc atttgcttaa attcatcttt agcttcatcg atagaagaac
    11641 aaattagtac acctttgcca gctgccaatc catcagcttt tagaactatt ggaggtgttt
    11701 gttgtgataa ataagctagt ccttcactta gcgttgccat tgtaaattct gcgtaagcag
    11761 cagtaggcac ctgatggcgc atcataaatg ctttagaaaa tgctttacta ccttcgagct
    11821 gtgcccctat ttttgaagga ccaataaccg gaatatgtct taattcagca tcattgacga
    11881 caaaatcata aataccttcc actaaaggtg cttcgggtcc tacgattatc atttttattt
    11941 cattttctaa tgcaaaagat ttaatagcgg caaagtccgt tgtagcaata ggcacattga
    12001 tacccaactg taatgttcct ggattgcctg gtgcaataaa caattggctg catttattac
    12061 tttttgcaat cgaatgagca aacgcatgtt ctctgcctcc tgagcctaat aataatatat
    12121 tcatgggaaa tttttgcgaa gatacttcct tttatctgaa tgccattttg tcctttcaga
    12181 tttttttgca caatatttgt catttcacat ttattatcgt tataaatgag cgtaaaatgt
    12241 taactttgca acctattttc aatatactat catgttaaat ttcttacaga aaggtctttc
    12301 caaaattttc ggcagtaaat ccaatcgtga cattaagtca ttagagccta tcatcgctgc
    12361 agtcaacgaa catttttatg catatcagaa tatatcgagt gatgaattgc gacaaaaaac
    12421 tcaagatttc aaattcagaa ttcaagggta tttatctgca atcgataagg aaataaatac
    12481 attaacagaa aaagcagaac aagataacct tgatcttgat gagaaagaag ctatcttttc
    12541 aaaagttgac aagctcaaaa aagaacgcga cgaagcgatt gaagaaatat tacaagatat
    12601 aatgccagag gcatttgctg ttgtgaaaga agcatcgcgt cgtttttcta ccaatgaaac
    12661 tattcgagta aaagcaacgg cattagatag agaattagct gtaaaaaatc ctcatataaa
    12721 aattgaaggt gacgatgctg tttttcccaa taaatggtcg gctgctggtg gcgaaatttc
    12781 ttggaatatg gtgcattacg atgtgcagct aattggtggt gctgttttgc acaaaggaaa
    12841 aattgcagag atggcgacag gtgaaggtaa aacattagtt tctacgcttc ctgcctattt
    12901 gaatgcactt gcaggctgtg gtgtacacat cgtaacggtc aatgattatt tggcaagacg
    12961 tgactctgag tggaatggca tgttgtttaa tttcttagga ttaacagtag attgcattga
    13021 caaacatgaa ccacatactg atggcagaaa gaatgcatat ttggcagaca ttacatacgg
    13081 cacaaacaat gaatttggtt ttgattattt gagagataac atggtgcgtt ctgctgaaga
    13141 acaagtacaa cgcaaactac atttcgcaat ggtcgatgag gtggactccg tattaattga
    13201 tgatgcaaga acacctttaa ttatttctgg tccagttccg aaaggggaag aacaaatgtt
    13261 ccaagaatta aaaccaagaa ttgaaagatt agttagcaca cagaaaaaag cagtacagca
    13321 atttttaaac gatgctaaac ggttaattgg cgaaggcaat gttggtgtaa gaaatggaga
    13381 aggcggttta gctttattac gagcgcatcg tggtttgccc aaaaacactg cattgattaa
    13441 gtttttaagt gaacaaggca ataagcaaat tttgatgaaa acagaaaatc attatttgca
    13501 agaccaacaa aaagaaatgc caacatgtga tgcagaatta tattttgtga tagatgaaaa
    13561 gaataatcaa atcgatctta ctgacaaagg tattgagttg attacgtcat ctggagaaga
    13621 aaaagatttc ttcgtaatgc ctgatgttgg tgctttaatt gcagaaattg aaaacgagcc
    13681 tgtatcatac gaggaaaaat taaaacgtaa agatgaagta ctattggatt tcaatatcaa
    13741 atcggagcga ttacataccg tcaaccaatt attaaaagcc tactgcttat ttgacaaaga
    13801 tgtagagtat gttatcattg atggaaaagt aaaaattgtc gatgagcaaa ctggtcgtat
    13861 catggaaggt cgtcgttata gcgatggctt acatcaggcg attgaagcaa aagaaaatgt
    13921 aacgattgaa gcagccacac aaacatttgc tacgattact ttacaaaact tcttccgcat
    13981 gtatcacaag ctttgtggca tgacaggtac agcagaaact gaagcagcag agttttggga
    14041 aatttataaa ttagacactg ttgtagtgcc aacaaacaga cctacacaac gcgacgataa
    14101 agaagatttg attttcaaga ctaaacgaga aaaatacaat gccattatcg aagaaataaa
    14161 taaacttcgt gaagaaggca gacctgtgtt agtaggtact actaatgttg aggtttctga
    14221 attattaagc cgaatgttga gcatgcgtaa aataccacac aatgtattaa acgctaagca
    14281 gcatcaacga gaagcagaaa ttgtagcaca agctggttta agtggtcaaa ttaccattgc
    14341 aacaaatatg gctggacgtg gtacagatat taagcttgga caaggtatta aagacattgg
    14401 tggcttagct attattggtt ctgaacgcca tgactctaga cgtgtggaca gacagttgcg
    14461 aggccgtgct ggccgtcaag gtgatactgg ttcctcacaa ttttattcgt cattagaaga
    14521 taatttaatg cgccttttcg gctcggatag aattgctgga atgatggata aaatgggcta
    14581 taaagaaggt gaagtcatac aacattcttt agtaacaaaa tcaattgaac gcgcacaaaa
    14641 gaaagtagaa gaaaatcact ttggcactcg aaaaagatta ttagagtacg acgatgtcat
    14701 gaatgcacaa cgcgaagtta tttacaaacg tcgtcaacat gcacttatcg gagagcgcat
    14761 tactttcgat ttaatcaata tgttttatga cttaagtgca gaaatttgta atgatgccaa
    14821 attatcacaa gacatagaag cttttaaatt agattgtatt cgctttttat ctgttgacac
    14881 atcaattaca gcagaagatt taaaaaacaa aacggaagaa gatttagccg cacatttata
    14941 ccatgaagtg tttgaaaaat atacacgtaa atctgaaaat attgccgatg aagcatatcc
    15001 aattattaag aatgtgtatg aaaccaaagg caatcaatac caaaatattg taataccatt
    15061 tactgacggc aatagaaaca taaacattat tgccaatctt gaaaaatcgt ataacaataa
    15121 aggacgcgaa ttgataaaag tatttgaaca aaatataatt ctttcattta tcgatcatca
    15181 ttggaaagac catttgcgtc aaatggatga gttaaaacac tcggtacaga tggccgttca
    15241 cgagcaaaaa gatccgcttt taatctttaa gttcgaagcc tatgagctat ttaaacaaat
    15301 gctgtctgct gttaataaag acgttacaac attcttgatg aaaggaagtt tacctgttca
    15361 agatgccaat caaatacaac agcacagaca attgcgacgt gcagaaagaa caactgaaac
    15421 acgtggcgat ggaacgactg aggaagaata tgctggtgcg gaaactgctg aaaccaaaga
    15481 aaagcaacaa cctgcacgtg cggaagtaaa aattggcaga aacgatcctt gcccatgtgg
    15541 aagcggaaaa aaattcaaac aatgccatgg caaagaaatg gcataatcgt tgatagatat
    15601 aattgaattg aaatatttaa tattcaatat gaaaaacgtg cttagcatat tatgtgtttg
    15661 tgttgcatcg acgtgtttcg ctgctgatag tgacagcaca gctattgaca taaaaaataa
    15721 tattttgttt tttaaagatc cgcgtgttga tgtattgcag aagatgtata tgcgaaaagc
    15781 tgtagatcca aatgcaaagg ttatacgagt gcaagttttt caggctattg ctcgtgataa
    15841 agtttttgag gctaaagcac aattctcggc tcgttatcca ggcatacaaa catttgtaac
    15901 gtatgcttct ccaaatttca gattacgtgt aggcgagttt agctcacaac aagaagcttt
    15961 taagtttatg caacaagtaa agcctcattt tcccatgagt tttgttattg aagagaaagg
    16021 cagtaaaaat gacgacaagc caaaatcaaa aagtgggaat tagtattccg aatcagaaac
    16081 atcttcatca acctgacccc aatttccagt gctaccatcg ttaatgatgc tttgaccact
    16141 ttcttcgcat aaatctttta ccaatgttga atctgaagga cggaaaaact tcgctgattt
    16201 attgagtgct aattttggat ctccatacgc tttttgcata aacataccga atacaggcat
    16261 agcagcacga ccaccttgac cgctactagt atataagaaa tgtaataaag gatcatcaca
    16321 gcctacccaa acaccagcca ataaatctgg tgtgaagccg ataaaccacg catcggtatt
    16381 tttatttgtc gttcctgttt tgcctgctac atctacatta gcataagaaa aataagctgg
    16441 caagctcgca cctgtgccac ctctggaaac gggtcctttc atcagctggc acattttata
    16501 ggctgtattg tcgctgaaaa cttctttcat ctctgttgta aaattctgga ttacatttcc
    16561 atctttatct tcaatacgta gtaaatactg cggttttaca ctaattccgc cacttggaaa
    16621 tgtgctatat acgttcatca tttcaaataa tgaaatatct gctgctccta atgctaatgg
    16681 tgaatatggt ggcaaatcag aggcaattcc catgcgtctt gacaaatcaa ttactggatt
    16741 tacgccaaac tgtttgatga tatatgcagc catctgattg tttgatgttt taagtgcttg
    16801 tagtagtgtt tgtggaccgc cactaccacc tcgtggtgcc caattgccaa tacgcaccat
    16861 gcctggcgca ataaccatac acggcgacca gccattatct acagctaaac catacactat
    16921 tggtttaaac gtagaaccaa cttgtctttt ggtactaggc ctacaatgat catttttaaa
    16981 aaattggaaa tttgggccgc cgacccatgc gcgaacatag ccagtataag gctccataac
    17041 catcattcct atttgcaaaa atgacttgta atattttaca gaatcataag gcgtcaatgt
    17101 ggtatctttc atgcctttgt aggtaaacac tttcatgcga tgtttatctg ttttgaagtt
    17161 tgctaaaatc gcatcattac tccaacctcg ttctttaagt gcagcccatc tatatgaata
    17221 tttacaagct gtttctaata tagttttacc agctttcgtg tcccaaggct tccagcttcc
    17281 accaaattga gaattaaaga taccttgata gcgagccaaa tgttgtactg ctgcctcttc
    17341 ggcatagcgc tgcatccttg tattgatagt ggtataaatt tttaaaccat cagtataaat
    17401 attatattct ctaccatcag gatttaggtt tttattttct ttggcccatt tttttaattc
    17461 ttcagctacc accattctga aataaggcgc caatccgtca tttgctgatt caggatgaaa
    17521 gtctaattct attggtttgt cctttaatgc atcaaattgt gctgtagtaa tatttccttt
    17581 ttccatcatt ttacggagca ccgttgctct gcgttcttgc gtgcgcttag caaatacttc
    17641 attccttcgt ggattataat aagatggatt ttgtaacatg cctacgaaca tggcagcttc
    17701 ttccactttt aaatctttag gttttttatt aaaataaatc ctagaagcgc tttcgatacc
    17761 aacggcatta ttgacaaaat cgaaagtatt aaaatacatt gcaataattt cttctttagt
    17821 gtatctgcgt tctaacatta cagcgattac gtattctttg gctttttgta gcacacgttg
    17881 tactatattt tgtgctctct tatgaaacat atttttcgct agttgctgtg ttaccgtgct
    17941 tcctccacca tgctgtccag caaagaatac agctgccaag gtacgtttta agtcgatgcc
    18001 agaatgctca tagaatcgct catcttcatt tgcaattaag gcttgcacta aaaatggtga
    18061 caattcttca tagcttatat tacttctgtt atctacataa tattttccaa tgagcacaga
    18121 gtctgctgag tagatttctg aagataaatt ggctgaagga ttttctaatt cttcaaaact
    18181 cggcaaggca ccaaacaatc ctatgcgaac taatgtaaaa aacaatacta cgctacccac
    18241 cataatggcg aatagtttcc ataaattttt tggtgttttc aaccagttta atacgttgtt
    18301 catttattta aataattggt tctaaaaaaa gtattgtaga tatctaagtc agcaatggtt
    18361 ttaatcagct tgaagtttga tggtgcaatg atgtaaaatg aagatttttg aacgtttgta
    18421 gcacctacag cttcgctcac attttcggta atcgtgttgt aatactgtaa tgcctctgcg
    18481 agtccttcaa attttttcat ctgcaagatg gcatagcttt ctatgaaaaa tgggtttatt
    18541 ttaattttta aatagtcaaa ttttttctta ttgaatttat gaatcgaaga gacaacatcg
    18601 gaaacggtaa tatatttatc ttttaattga accaaaacaa ataaatctgt tgtatccata
    18661 acaaacttag aacttatagt atctagcgtt tttggcgtgt tgctattctg tattgcttgt
    18721 gtagaagtgt cattcagttt gttttcatgt tgtatgtaag caatcaaata ttcctgtgct
    18781 ttctttttta catcaccatc attatatttt agaataatat cagacaatgc ttttttgtat
    18841 gcttggaatt gttttatctt gccataagaa agtgcttcta acatttcgaa ttgtgcttgc
    18901 tgtggcgcat ttggatattt tgttttggcg acaaatctat ttttgataac atcatcaaat
    18961 ttatcattta gaaacaattg ataggtcgaa gcatacagtt tttcagctgt ttcaatactt
    19021 tcatcaacta cacgaatttt tccttcatta ttgagcactt ctgctacttc tgtcatcgga
    19081 tagttttgca agatatagtc tttttctttt tgcgctaatc catttagatt ttgttctttg
    19141 taaatcagat attcgtaata atgagcgatg gcatcgaact cactattcgg aaacctacgt
    19201 attaattcgt caaatgcttg ttttgattta ggaatattat tcaatttatt cttgaaaatt
    19261 tctcctgtac cataaagtgc gttagcaatt tttatatcag agcgcagaaa atcttctggt
    19321 gtttttggaa tctttaaagt atctgttgtt gttgttgttg cttttggaat atttttattt
    19381 ttcacagcag cagttactgt atcattaata gctgtctcat ctgttgatat tatatcttct
    19441 aagaaaccag atttgacgct tcgtcgccaa ttatcttcta attttcggtc gccccaaatt
    19501 cgtaaaaaat cattatatcc tttgctctta gattctggat tataaaaata ccacaagcca
    19561 ttagacacgc cagtttcagg cacgaatgca cttgcagcac ttgtgttacc atcaccaata
    19621 tctgagctga atcgagattt ctttttatcc gctttttctt tttgagcctt tacttctgct
    19681 aaaaatttat ccaattcttt tggtgacatt ctgcctaatc tttgcaagct atcttccgtg
    19741 taaattgttt gtgcatacgt tgctaaatca cctaacacct ctttgcgtgt tacaatttgc
    19801 tgataatttt caaaagtatt tggcaagtaa gcaatcgcac tatcataaaa tatggccgct
    19861 ttatcgtagg cttctttatc aaaataaatt tctcctaaac gcaaatatga tttacctttt
    19921 tgctccttat tgtcgagcga tttttttatc gatttttcta agtatgctac tgcatttgct
    19981 gtgtcttttt gataataata gtaattagca atcgtgtaca atacttcatc tgcatgattt
    20041 ttattcttgc caaattttat aagcttggac aacattttca caatatcttg attggctaat
    20101 ccaggattat cgctatacag ttttgccaat tgtatttttg aaaacaaata aagttcttcc
    20161 gatttggtat gcttaccagc ttttacaaaa taatctgctg catcggcatc ttgattgttc
    20221 agttgataaa gttgtgctaa taaatattcg taataa
//
LOCUS       DAOQQV010000037        19987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_812_length_19987_cov_7.804385, whole genome shotgun sequence.
ACCESSION   DAOQQV010000037 DAOQQV010000000
VERSION     DAOQQV010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19987
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_812_length_19987_cov_7.804385"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLL59_05590"
     CDS             <1..618
                     /locus_tag="PLL59_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMY23312.1"
                     /translation="DFVAAGEYTSAEEALIPIKEKKPDVIFLDVDMGEMSGIDLRSQL
                     PEIPVCIFITSHPEYAVDGFEQNALDFLTKPLQKDRFERSITRARQYLQIKQKATLLD
                     ENIGSDSIFIKDGHEKIKIQTHEILFLEALRDYTKIVTSNKSHFVLTGLSAILSEKAF
                     SSFIRIHRSFAVPRHYIKMIKSNEVIIEKYAIPIGRSYKMQLQNL"
     gene            645..2390
                     /locus_tag="PLL59_05595"
     CDS             645..2390
                     /locus_tag="PLL59_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMY23313.1"
                     /translation="MGPIIALGHSYQRETDSIRQLIKTSVSETKKIELYTQLGQYCIE
                     SNNHTQALDYFILALNLCKQPMQDSLKIVCYKQISRIFFYQKNFAKSDHYNNLALKLS
                     KENPLLEGDYYKRKGDAFTSQLMYDSAQFYYAITENLYKKSNNTDSLRIAGLYANLSI
                     TYYETDKVKSIEYAYKAKDFFGKNRDSKYYINQGNIGNFYKDIVRFNVYDSLSKLSTH
                     VSSSKKTCIQKGYFFIQDAITLARVSGNRTDEAYYTGILSELQEVDGDYKSALLNFKT
                     YYEISDSVYSQDIKNELAEKESALEIEKKNQEIALKQIQLTSQKRLAIALTTGILLLS
                     VIGLLLYFQARNRKKNNILLNRLNADLSHANEIKTKLFGILSHDLRSPIANLITLLQL
                     KRDGSFDTKNEEQVYTDKVILSAEHLLENMETLLLWSKGQMQNFKPNYQNILISELFR
                     YIAIFFEHEKQVLIQFNNPDHLQIISDEIFLQIIMQNLTSNAIKSLKNTDQALICWNA
                     NQTEDGIMIQITDNGPGMDENKLNEILVSNQTLSQKSGLGLQIVNDLAKAINCKLHFK
                     TALNKGFSVTIIINK"
     gene            2490..3896
                     /gene="lpdA"
                     /locus_tag="PLL59_05600"
     CDS             2490..3896
                     /gene="lpdA"
                     /locus_tag="PLL59_05600"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMY23314.1"
                     /translation="MNKHDITIIGSGPGGYVAAIRCAQLGFNTALIEKYNTLGGTCLN
                     VGCIPSKALLDSSEHYHNALHTFKEHGIELQETPTVNFEQFIARKSKVVETTCGGIDF
                     LMKKNKITVYHGHGSFVNATTIAIKKEDGSEEQIESAKTIIATGSKPSTLPFLPIDKT
                     RIITSTEALSLKELPKSMVIIGGGVIGLELGSVYQRLGTQVSVVEFMDKIIPTMDDDM
                     GKELQRSLKKLGIKFYLSHKVTGAVNNGDSVTVTALDKKEQEIKIDADYCLVAIGRRP
                     YTDNLGLENIGITLNERKQIPVDAHLQTVVPNVYAIGDVIQGAMLAHKAEEEGVFVAE
                     TIAGQKPHINYNLIPGVVYTWPEVAAVGQTENQLKEKGIAYKVGSFPMRALGRARASM
                     DIDGMVKVLADANTDEILGVHIIGARAADMIAEAVVAMEFRASAEDISRISHAHPTYT
                     EALKEAALAATANRAIHS"
     gene            3915..4481
                     /locus_tag="PLL59_05605"
     CDS             3915..4481
                     /locus_tag="PLL59_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23315.1"
                     /translation="MQTINHPQLVKLLQQAYSAERAASFAYQGHAGSVKNKQEKNTIK
                     QIETDEWNHRAEVLKIMQQYNIPVSTWYEIKFYIIGKIISFSCYIIGWFMPYYFAGRL
                     ESGNVCEYFRMMKYFHEIGITTHNTILFEMGIKEKEHEIYFLNSIENKKLLPFFEKIF
                     AWGNHKSFNDIDLENKYPIEESSKYCGL"
     gene            4735..5157
                     /locus_tag="PLL59_05610"
     CDS             4735..5157
                     /locus_tag="PLL59_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23316.1"
                     /translation="MKKTLIFFLLFIGAATVMTSCKKTAAQTEKERVLELLTSGKWYY
                     QSYQNLPDGEIYTCFNAVDYFEFETDGSFEETWDFGNSTYTIAEDGKSIVLNLGEGGA
                     IPTTATVTITETSLKLVIDWDEGGETEYIFSKTASVCL"
     gene            complement(5231..5689)
                     /locus_tag="PLL59_05615"
     CDS             complement(5231..5689)
                     /locus_tag="PLL59_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMY23317.1"
                     /translation="MALPNVCDPQVAQNLIHRIEQVTAETQPLWGKMNASQMLAHCNV
                     TYEYIFEERTDKPNFLVKWLLKTFVKKKVTSELPYEHNMATSPAFIIPDTKNFTQEKT
                     RLINYINKVVSNGNKYFEGKSSNSFDVLTTEEWNNMMYKHLDHHLRQFGV"
     gene            complement(5712..6305)
                     /locus_tag="PLL59_05620"
     CDS             complement(5712..6305)
                     /locus_tag="PLL59_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23318.1"
                     /translation="MFRLFQLSILSIVFILHFSCAKVEDPTNDNPDVVSVQIDSTLSS
                     FYPAAGPGFESIFSFTIPNTTSFDIDLYWYPTTPHGIYLHFNNLNGDVLIDNNGCILA
                     FDSGIKIDSTLAGNWSDTTDGAFAYDYVSNPSANKGNLAGKGDKYIVFRTFSNIEPQF
                     KYYAWMRVRVSENGRSVQVLSIAYQKNPNICLRTGEL"
     gene            complement(6339..7559)
                     /locus_tag="PLL59_05625"
     CDS             complement(6339..7559)
                     /locus_tag="PLL59_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HMY23319.1"
                     /translation="MRHIYIFLFATFLFFSCKQNDIQPTPFEYDGALGDITKIISETP
                     LSISDIFAELPSDITSFLTLKSNITAYVVEYKSQNKDGDTVIASGIIIIPNTENIAIP
                     LLSYQHGTILPKNSAPSIHKGEEYKLNLALSSSKDVVTCIPDYLGLGTGEGQHLYLNP
                     REEANSVRDILRAARKLVKQKQSTALNGQVFLYGYSQGGHATMAAQRQLELENSNEFK
                     LTASAPMAGPYAMSRTSQFNVMLDSIYYPNPFYLPYLAVSLFKTYPLYASYSQLFKSP
                     FDARIPVAIDGYHSYSYANSQFDYYVSNIILDSVKANIRTNPNHPIRLACKGYDLVDD
                     WTPTTSMRLYHCEGDDNVYYDNAVYADSVFRARGADVELVNLGSDNHTDCAPTAIFVT
                     MSWFSGLFEYTKIK"
     gene            complement(7721..8773)
                     /locus_tag="PLL59_05630"
     CDS             complement(7721..8773)
                     /locus_tag="PLL59_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HMY23320.1"
                     /translation="MTYIKSQFAHPHLPKNKLGYTRKYYEGALSTLCAGCGHDSIGAA
                     IVEACWQMNIEPHKVAKLSGIGCSSKTPAYYLNNSHGFNSVHGRMPSIATGANLANKD
                     LIYLGVSGDGDSASIGMGQFVHVIRRNLNMTYIVMNNGCYGLTKGQDSATADVGSKSK
                     SGNTNMFEGIDLCSLAIELGATFVAQSFSGDKTQLIPLIQAAMSHQGFALINVMSPCV
                     TFNNNVGSTKSYDFVREHVEATGTIDFVPPRTEITASLHDDETKAIEMHDGSWLSLHK
                     VAQSWDPDDKIAAMNALKLAHAKQEILTGLLYIHTASKDLHTLLNTTDTPLNKLDETT
                     LCPGVAVLEEINAEFR"
     gene            complement(8770..10605)
                     /locus_tag="PLL59_05635"
     CDS             complement(8770..10605)
                     /locus_tag="PLL59_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HMY23321.1"
                     /translation="MSLSKVNNFVVRFANVNGTGSASANQLFAKSIFRMGIPVTPKNI
                     FPSNIQGLPTWYEVRVSEKGYLGRREGIDLMLCVNPQSMLKDVQSVKSGGYFMYDNTK
                     PLHKEYHRDDITFIGIPLTAICNKEYSDPRQRQLFKNIIYVGALSALLAIEFEVLETL
                     VTEQFKGKEKLIAPNIHALQLGVNYVKENFSYPLDIQVERRNLIKDKILLNGNTAAGI
                     GAVYGGASVVSWYPITPSTSLIEAFEDYAILYRNDKETGKKNYAIVQAEDELAALGMA
                     IGANWNGARSFTATSGPGLSLMNEFLGLAYYSEVPVVLVDVQRSGPSTGMPTRTQQSD
                     ILLAAYASHGDTKHVLLIPSTPAECFEMTATAFDLAEFLQTPVMVMLDLDLGMNDHIS
                     APLQWHPDRKYNRGKVLSAEQLENIERFGRYLDIDKDGVPYRTIPGTHATKGAYFTRG
                     SSHDEYAVYSEDSATYVRIMERLLQKFETAKSMVPAPIFYQKDNQHEIGFIFYGTTTA
                     AAEEAMDILNTQGISIDAIRIKAFPFIDDVLNFINAHNEVFVIEQNRDAQMRTLLINE
                     LEIAPSKLKKILNFDGMPITADFIVKQTIAQLPKSLLNNKETLSV"
     gene            complement(10596..12371)
                     /locus_tag="PLL59_05640"
     CDS             complement(10596..12371)
                     /locus_tag="PLL59_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY23322.1"
                     /translation="MEPTNVNDPEYYHKVVDCQHACPAHTPVPQYIRLIAQEKYDEAY
                     LVNWESNVFPGVLGRTCDRPCEPACRRGRLADEEPVAICRLKRVAADNKGNIQHLLPI
                     PETANGKKIALIGGGPASLTVARDLALLGYELHLYDDHNAAGGMMRSEIPSFRLPDEV
                     LDEEVNQILQYGIHTHFNTYVNSLAEIINQDYDAIFIGTGAPRGKNLNLPGRQEAKEK
                     IHIGIDWLANVAFEHTHKIGKKVIVLGGGNTAMDCCRTARRLGGEAVKVIVRSPRSEM
                     KASPWEIEDAVHEDIPIIENHVPKSFVIKDAQLIGMTFDIVKASYDTNGKRTLTSTGE
                     EAFYEADEVLIAIGQENSFPWIERNLGIEFNEWNLPIVDSNTFQSTLPKVFFGGDAAL
                     GPKNVITAVAQGHQAAISIDLFCQNKNVQDRPAPFTTLVSTKMGIHEWAYDSIVSNDD
                     RYIVPQAAKAVVLQNRKMEVELGFNLPTGFKEAQRCLNCDVQTVFTATKCIECDACMD
                     ICPTNCITFTFNDEEEKLREKLKAPALNLDQSLLVSSTLTTGKIMVKDEDVCLHCGLC
                     AERCPTSAWDMQEFLYKTSKAGSCH"
     gene            12822..14531
                     /locus_tag="PLL59_05645"
     CDS             12822..14531
                     /locus_tag="PLL59_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMY23323.1"
                     /translation="MEGFRALGLSDEILDALEKKGYESPTPIQAQTIPLLLNGNLDVV
                     GQAQTGTGKTAAFGLPILDRFDPAQKSVQALVLAPTRELAIQVAEEIKSFKGAKRIWI
                     TTVYGGAPIGAQMRELEKGTHVVVGTPGRVIDLIERKKLVLDEVKYVVLDEADEMLNM
                     GFIDDIELILKSCNETRRMLLFSATMPDRILSLAKRYMGKFEMVRIAKQEITNVNIEQ
                     SYFEVPDKDRFDALCRVLDVEEDFYGIIFCNTKAEVVEISNHLNDKGYTSDALHGDIQ
                     QNLREKVVKQFKTNKFGVLVATDVAARGIDISDLTHVINYGLPQDPESYVHRIGRTGR
                     AGKEGKAIAIISPSEKRKLLFVERIAKTKMKKGKLPDANDMVKIKTRRLTEHVETIIG
                     TEHYSEYLELAAQLIGDNEVEVIVAALIKHFAKDELNPKAYKQIKEAAPREEFGNRFE
                     KGRRDDRGGRRDDRSSRRDDRNDRHDRRDERGGNRRGGSAQRLFFAKGRADGMTPRAL
                     LDELKNTAGIESRRIDDIRILDNFSFFATNKEDAEQILAVYKRNKGNGKPMVDKARER
                     DDR"
     gene            complement(14619..15053)
                     /locus_tag="PLL59_05650"
     CDS             complement(14619..15053)
                     /locus_tag="PLL59_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23324.1"
                     /translation="MKTKIKQFIPILLLLFVISINTGCKKDKIKIQTQLPSNYTNQGT
                     INVVNKNISICIYDNSVEDGDIIDLLFNGNTLISDYEILNTERCFTVSLENGDNWIGI
                     NVDNEGSNPPASVTVKINDGTSEQEFDIDGEVDAPGGYIIKL"
     gene            complement(15058..15516)
                     /locus_tag="PLL59_05655"
     CDS             complement(15058..15516)
                     /locus_tag="PLL59_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23325.1"
                     /translation="MSKKIIDKETGTLTLYNGFIISKDTTLENLLNTFTSNELKKSEY
                     ADNICSLKQAKYDEYYIRFWFYFIEEKIYKIGFEIETKPIQRVPWSNQRDLETKWIAE
                     QMDDKSNFIWDMNIAGRHYSLKYNWGFIGVYYDFKNGTFDSSINYHSLKK"
     gene            complement(15559..16602)
                     /locus_tag="PLL59_05660"
     CDS             complement(15559..16602)
                     /locus_tag="PLL59_05660"
                     /inference="COORDINATES: protein motif:HMM:NF016973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm10 family immunity protein"
                     /protein_id="HMY23326.1"
                     /translation="MLALEQYAALYLSMIAATGNNEQEQQICSSNGITIAQWQEAHTY
                     YTAKMSDPMDMGKTAMAFSAAMTNAKPIIPIPANFIASNVKIYISEYDVQMIEFTNNA
                     SKQHIILQLGFEAKDDFEKNYINGRVHISINDQSHSLYGGVSKVELSSKNIIFSFDEE
                     GKTRMNCNSIEVVFDINRKYYNLLKRKMQFMYKTILTIKDEEIPTTYNVNGILLSDEW
                     ISFTPNNNIKISIRPNLQNIKETGKYNQVVFVDFRSNTIGQDANETALLTEMEACLTD
                     TIEKDLAAVIAFHVTDQEKRRFFIYTYLSQDDFMMRINDAFKLLPKMPLAFSGGVDAA
                     WENYKNCLADLKN"
     gene            complement(16621..17190)
                     /locus_tag="PLL59_05665"
     CDS             complement(16621..17190)
                     /locus_tag="PLL59_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23327.1"
                     /translation="MKKLWSYIFVCSVFFTACKTNKPDSSETTIEQNIDEIKSSTTEQ
                     GKAGKGKITLNCIDKTYEINGVCGAVSSMGTLTIAVTDDSIPAKSFTINFNTDKLPEN
                     SSSYTIVKYNVEDKNATNISISYADMRSTDQMLWESNNKTGKLYFVVNGNEIKCTFSK
                     LTLQPSMMYNKGGLDAPATVSGELTIYNN"
     gene            complement(17200..17655)
                     /locus_tag="PLL59_05670"
     CDS             complement(17200..17655)
                     /locus_tag="PLL59_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23328.1"
                     /translation="MKTTIQTIQIVLFLFLAVATTTACKKDKNSAGKTGTFTIDGVSY
                     TGNTEVQTFVNNNYSIVCQQDAPFKLIQVTFHNQAEAEAGGTFDVEDDALNVPSGSVN
                     VGVDGLTFDPDGSQTISVSGKKITINNLALDQTGGGSKHPLINNASINF"
     gene            complement(17710..18642)
                     /locus_tag="PLL59_05675"
     CDS             complement(17710..18642)
                     /locus_tag="PLL59_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23329.1"
                     /translation="MKTKILSLMIVASLSTLTACKNENTPEPAVNLKGKIAPSLSMEK
                     DGEIEFFDLRDVNNPKKAAFNIIGTADNFYLQYVKSRPIGSKTIKANYGDLSINHNKE
                     MYLADKNWPAWKNISTVGDWLIMNNDNTNQLYPAYQDYEDSTEIYSYTNFFDDFASKP
                     NWGNAGIINGCAEVYENYNSILSTRNAFYFNFKTQEYLFDYNINNSGGIYTGYKITDL
                     VKKPNGNMDTDPIDWTKADFVIALSYNHSMQTLTYNGKNVTALVFVDMDTKTYAYFLR
                     NAVDDVVNGADKGRQTLMTITSQPLSNLFKGWDL"
     gene            complement(18671..19516)
                     /locus_tag="PLL59_05680"
     CDS             complement(18671..19516)
                     /locus_tag="PLL59_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23330.1"
                     /translation="MKNLKHRISIVLLIFFFSSCKKEETASETWQFTKMVNITKDNSG
                     ALLSIDFYQKKGNQWRIVQMEPDDYTKFYLKKIVTPNAYQLPIENENAFMTPNVRLFT
                     PDNKVYDPGNIKWLLGEPIYFKTFNTFSYEFPDMPSQYADVSKFSAATYSYEQVYDNN
                     GKNDSYIFYDFSNQKYLYYGFRAGADLILENALTILCPSCNSIDWKNIDAATCTVNYS
                     TAEDLYYFFDFDAKKFYIITRINKNSNNPSFTFDTGLVINFNEAFFNEAGSNVGNELP
                     FDFSK"
     gene            complement(19660..>19987)
                     /locus_tag="PLL59_05685"
     CDS             complement(19660..>19987)
                     /locus_tag="PLL59_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23331.1"
                     /translation="PFPQNYVLKTIKSIYFYLIQDFDTALSIISNVIQSVASDKMSHH
                     KTISLLYKKLYTAKQKHVLQKKLSKKDVKILQDSIIEFEEKTPTEFKLVSVYLWLKQE
                     IEQNLV"
BASE COUNT         6406 a         3430 c         3514 g         6637 t
ORIGIN      
        1 gactttgttg ctgcaggtga atatacttcc gccgaagaag ccctgattcc aatcaaagaa
       61 aaaaaaccgg atgtcatttt tttagatgtt gacatgggtg aaatgagtgg catcgacctg
      121 agaagccaac tgcctgaaat tccggtttgt atttttatta ccagtcatcc tgaatatgct
      181 gttgatggct ttgaacaaaa tgcattggat ttcctaacga aacctttaca aaaagaccga
      241 tttgaacgaa gtattacaag agcaagacaa tatcttcaga taaaacagaa ggcaacacta
      301 ctggatgaaa atatcggatc agacagtatt tttataaaag acggtcatga aaaaattaag
      361 attcaaacac acgaaatctt atttctggaa gcgctgagag attatacaaa aattgtaaca
      421 agcaataagt cgcattttgt cttaaccggt ctttctgcca tattatcgga aaaagcattt
      481 tcttcattta tcaggattca cagaagtttt gcagtaccca gacattatat taagatgata
      541 aaatcaaatg aggtcatcat agaaaaatac gccattccga ttggaagatc ctataaaatg
      601 caactacaaa atctgtgaac cgattcatta tactatatct tttattgggt cctattatcg
      661 cactgggaca ctcttaccaa agagaaactg actcgataag gcaattgatc aagaccagtg
      721 tttccgaaac gaaaaagatt gaactatata cgcaattagg tcagtattgc attgaatcaa
      781 acaaccatac tcaggcatta gattatttca tactggcgtt gaacttatgt aaacaaccta
      841 tgcaagacag cctgaagata gtgtgctata agcagataag cagaatattt ttttaccaaa
      901 aaaattttgc caagagtgat cattacaaca atcttgcctt aaaactatca aaagaaaatc
      961 cgttactgga aggtgattat tataagcgca aaggagatgc gtttacctcg cagcttatgt
     1021 acgactctgc acaattttat tatgcaataa cagaaaattt atacaaaaaa tcaaacaata
     1081 cggattcctt acgtattgcc ggtttatatg ccaacttaag tatcacttat tatgaaacag
     1141 acaaagtaaa atccattgag tatgcttata aggcgaaaga cttttttggc aaaaacagag
     1201 acagcaaata ttacattaat cagggaaaca ttggtaattt ctacaaggac atcgttcgat
     1261 ttaatgtata tgacagcctg tcaaaactat ctacccatgt ttcttcatca aagaaaacct
     1321 gtatacaaaa aggatatttt ttcattcagg atgccattac actggccaga gtatctggca
     1381 acagaactga tgaagcgtac tacacaggaa ttctatcaga attacaggaa gtagatggag
     1441 attataaatc agccctactt aattttaaaa cctattacga aatatcagat tcggtttatt
     1501 ctcaggacat aaaaaatgaa ctggcagaga aagaaagtgc cttggaaata gaaaaaaaga
     1561 atcaggaaat agcattaaaa caaatccagt taacatcgca gaaacgcctg gcaattgcac
     1621 ttacaacagg tatcttatta ctttctgtaa taggattatt actttatttt caggcgcgca
     1681 acagaaaaaa aaataacatc cttcttaatc ggttaaatgc tgatttatca catgccaatg
     1741 aaataaaaac taaattattt ggcattctca gccatgacct cagaagtccg attgcgaatt
     1801 taataacact cttgcaatta aaaagagatg gtagttttga cactaaaaat gaagaacagg
     1861 tatacaccga taaagtaatt ctaagtgccg agcatctttt agaaaacatg gaaactttat
     1921 tgttgtggag caaaggtcaa atgcaaaatt ttaagccaaa ctatcaaaat attttaattt
     1981 cagaattatt tagatatata gcaattttct tcgaacatga aaagcaagtg cttatccaat
     2041 tcaataatcc agatcatctg caaattatat ctgatgaaat ctttctacag ataatcatgc
     2101 agaatctgac ttctaatgcc attaaaagtt taaaaaatac agatcaagca ctcatttgct
     2161 ggaatgccaa tcaaacagaa gatggcatta tgattcaaat aacagataat ggaccaggaa
     2221 tggatgaaaa taaactgaac gaaatattag tatccaatca aacactaagt caaaaaagtg
     2281 gattaggttt gcaaattgtt aatgatttag ctaaagccat aaattgcaag ttacatttca
     2341 aaacagctct taataaaggc ttttctgtaa ctattattat caataaataa caacatttca
     2401 caacaataaa aaaatttatt gataatttca ttcttttatt tgaatcttta ttgccgtatt
     2461 tttgttacat aaaaaaataa gaaatttaca tgaacaaaca cgatatcact attataggca
     2521 gcggccctgg tggctacgtg gcagccattc gttgcgcaca attaggtttt aatactgcac
     2581 tcattgaaaa atataatacg ctcggtggca cttgtttaaa tgtaggttgc attccatcta
     2641 aagctttatt agattcatca gaacattatc acaatgcttt gcatacgttt aaagaacatg
     2701 gtattgaact acaagaaaca ccaactgtaa actttgaaca atttattgca cgtaaatcta
     2761 aagtagtaga aaccacttgt ggaggcattg attttttaat gaaaaaaaat aaaatcacgg
     2821 tctatcatgg gcatggttcg tttgtaaatg ctactactat tgctataaaa aaagaagatg
     2881 gcagcgaaga acaaattgaa tctgcaaaaa ctataatagc aactggttca aaaccatcta
     2941 ccttgccatt cttaccaata gacaaaacaa gaattattac atcaacagaa gcattgtcat
     3001 taaaagagct accaaaatct atggtcatta ttggtggtgg tgtaattggc ttggaattag
     3061 gttctgtcta tcagcgttta ggaacacaag tttctgttgt tgagtttatg gataaaatca
     3121 ttccaactat ggatgatgac atgggaaaag aattgcaacg cagtttgaaa aaattaggca
     3181 ttaaatttta tctatcacat aaggtaactg gtgcggtaaa caatggcgat tcagtaacag
     3241 taacagcatt agacaagaaa gaacaagaaa taaaaattga tgctgattat tgtttagtgg
     3301 ctatcggcag aagaccatac accgataatt taggtttgga aaacattggc atcaccttga
     3361 acgaaagaaa acaaattcct gttgatgcac atttacaaac tgtcgttcca aacgtctatg
     3421 cgattggcga cgttatacaa ggcgcgatgt tggcacacaa agcagaggaa gaaggtgttt
     3481 ttgttgcaga aactattgcc ggacaaaaac cacatatcaa ttacaatctg attcctggtg
     3541 tggtttatac ttggcctgag gtagcagccg ttggacaaac agaaaaccaa ctaaaagaaa
     3601 aaggcattgc ctacaaagtt ggctcatttc cgatgcgtgc cttaggcaga gcgcgtgcca
     3661 gcatggatat cgatggtatg gtaaaagtgt tagcagatgc caataccgat gaaattctgg
     3721 gcgtacatat tattggtgct cgtgctgccg atatgattgc agaggctgtg gtggccatgg
     3781 aattccgtgc aagcgcagaa gacatcagta gaatttcaca tgcacatcca acttataccg
     3841 aagctttgaa agaagcagca ttagcggcta cagcaaatag agcgatacat tcgtaaaaca
     3901 ttataggcga atcaatgcaa actatcaatc acccgcaatt agtcaagctg ttacagcagg
     3961 cttattctgc ggagcgtgca gcatcatttg cctatcaagg tcatgcgggt tccgtaaaaa
     4021 ataaacaaga aaaaaatacg ataaagcaga tagaaacaga cgaatggaat catcgtgccg
     4081 aagtattgaa aattatgcaa cagtataata ttccagtttc tacatggtat gaaattaaat
     4141 tctatattat tggtaaaata atttcttttt cttgttacat cattggatgg tttatgccat
     4201 attattttgc aggaaggctc gaaagtggca atgtttgtga gtatttcaga atgatgaaat
     4261 attttcatga aataggcatt actacgcaca atactatttt atttgaaatg ggcataaaag
     4321 aaaaagaaca tgaaatctat ttcctaaaca gcatagaaaa taaaaaacta ttgcctttct
     4381 tcgaaaaaat atttgcttgg ggaaatcaca aaagttttaa cgacattgat ttggaaaata
     4441 aatatccaat agaagagtcg agcaaatatt gtggtttata aaaattgcct atcatatttt
     4501 ttgtgaaata ttttcccgct aaaaattgct taattatttc attagcctaa ctctcataac
     4561 agttagcaat cagtaatttc ccaactacaa tttcacgaag tttatcatat ttcttactgt
     4621 tcataaagtc aaattgacca ttcgtgaatt ggacaactag attgaatacc tattgctgca
     4681 tctttgtatt acaatattat taaacacatt tttaaacctt aaaaaacaaa caaaatgaaa
     4741 aaaacactta tctttttctt gttattcatc ggcgcagcga cagtaatgac atcttgcaaa
     4801 aaaacagcag ctcaaactga aaaagaaaga gtattagaat tattaactag cggtaaatgg
     4861 tattaccaat cttaccaaaa ccttccagac ggcgagattt atacttgttt caatgctgtt
     4921 gattattttg aattcgaaac ggatggttcg tttgaagaaa catgggattt tggcaatagc
     4981 acttatacca tagcagaaga tggaaagtct atcgtattaa acttaggaga aggtggtgct
     5041 ataccaacga ctgcgactgt aactattact gaaacttcat taaaactagt tatagattgg
     5101 gatgagggtg gcgaaacaga atatattttt tctaaaacag catcggtttg tttataaaaa
     5161 ccaagcaaat aaaaaactaa aactatatag agttaagata gcgaagcatt tgcttcgcta
     5221 tcttttttaa ttatacacca aattgtctta aatgatgatc gagatgttta tacatcatgt
     5281 tgttccattc ttctgtcgtt aatacatcaa acgaatttga agattttcct tcgaagtatt
     5341 tatttccatt gctcactact ttgttgatgt agtttattaa gcgtgttttt tcttgtgtga
     5401 aattttttgt gtcaggtata ataaaagcag gcgatgtagc catattgtgc tcgtaaggta
     5461 gctcgcttgt tactttcttt tttacaaatg tttttagtag ccattttact aaaaaattgg
     5521 gtttgtcagt gcgctcctca aagatatatt cgtacgttac attgcaatgt gccaacattt
     5581 gtgaagcatt catcttgccc caaagtggct gtgtttccgc tgtaacttgt tctatccgat
     5641 ggataagatt ttgcgccact tgagggtcgc atacgtttgg tagtgccata gctttaattt
     5701 ttataaattt actataattc gccggttctt aaacatatat ttggattttt ttgatacgct
     5761 atcgataaaa cttgtacact tcgcccattc tcgcttacac gaactcgcat ccaagcataa
     5821 tatttgaatt gcggttcaat gtttgaaaat gttctaaata ctatgtactt atcgcctttg
     5881 ccagccaaat ttcctttgtt agcactcgga tttgatacat aatcgtaagc aaacgcacca
     5941 tcagttgtgt cactccaatt gcctgcaagt gtggagtcta ttttaatgcc agaatcgaat
     6001 gcaagaatac agccattatt atctatcaac acatctccat ttaaattatt gaagtgcaaa
     6061 taaatgccat gtggcgttgt tggataccaa tataaatcga tgtcaaaact ggtcgtgttt
     6121 ggaatagtaa atgaaaaaat actttcaaac cctggccctg ccgctggata aaaagagctt
     6181 aaagtactgt caatttgtac agaaacaaca tcaggattgt cattcgttgg atcttctact
     6241 tttgcacatg aaaaatgaag aataaatacg atagaaagta ttgaaagttg aaatagtcta
     6301 aacatatagc aaagatacgc tttatcaatt aatgcgttct atttgatttt agtatattca
     6361 aataaaccac taaaccagct catcgtaacg aatatcgcag taggcgcaca gtcggtatgg
     6421 ttgtcgcttc ctaaatttac taattcaaca tcagcaccgc gcgcacgaaa aacggaatcg
     6481 gcatacacag cattatcata atatacattg tcatcgcctt cacaatgata taatcgcatt
     6541 gacgttgttg gcgtccagtc atctaccaag tcataacctt tgcacgctaa gcgtattgga
     6601 tggtttgggt ttgtacgaat gtttgctttc acagagtcaa gtataatatt gctgacataa
     6661 taatcaaatt gcgaattggc ataactatag gaatgatagc catcaatggc aacaggaatg
     6721 cgtgcatcaa atggcgattt aaaaagctgg ctgtaagatg catataatgg ataagttttg
     6781 aataaactca cagctaagta aggcaaataa aatggattag gataatatat agaatctagc
     6841 atgacattaa attgcgatgt gcggctcatc gcatacggac ccgccattgg cgcacttgcc
     6901 gtgagcttga attcattact gttttctaat tctaattgac gttgtgcggc cattgttgcg
     6961 tgtccgcctt gcgaatatcc atataaaaaa acctgaccat tcaaggcggt actttgcttt
     7021 tgcttgacca gcttacgtgc tgctcgtaat atatcacgaa ccgaattagc ttcctctcgt
     7081 ggattcaggt ataaatgttg tccttcgcca gtgcctaaac ctaaataatc aggaatacat
     7141 gttaccacat ctttactgct ggataatgct aaattcaatt tatattcttc gcctttatgt
     7201 attgatggcg cactattttt tggcaagata gtgccgtgtt gatagctaag taatggaata
     7261 gcaatgtttt ctgtgtttgg tataataata atgccactcg caataacggt atcgccgtct
     7321 ttattttgtg atttgtattc gacgacataa gcagtaatat tgctttttaa tgttagaaat
     7381 gaggtgatgt ctgaaggtag ttctgcaaaa atatcactta tagaaagtgg agtttcactt
     7441 attatttttg tgatgtctcc taatgcacca tcatattcaa atggtgttgg ttggatgtca
     7501 ttttgcttac atgaaaaaaa taaaaacgtt gcgaatagaa aaatgtatat gtgtctcata
     7561 gctaatgtgt tttatcaaat gtagcgaaaa tattgcgaaa gataaaatca ttgctttttg
     7621 gattgttatt aaaagacaca ataaataatg ttcgttttgg tgaggacacc aagcgataga
     7681 aaaaggctag gctattaaag taaattgtgc tagatgataa ctatctaaat tcagcattga
     7741 tttcttctaa cactgcgacg ccaggacaca atgtagtttc atcgagttta tttaatggtg
     7801 tgtctgtagt gtttaataat gtatgtaaat ctttacttgc tgtatggata tacaataaac
     7861 ctgttagtat ttcttgttta gcatgtgcca atttcaacgc attcatagcc gcaatcttat
     7921 catcaggatc ccatgattgt gctaccttgt gtaagctcaa ccacgaacca tcgtgcattt
     7981 caatggcttt tgtttcatcg tcatgtaagc tggccgtaat ttctgttctt ggcggcacaa
     8041 aatcaatggt gcctgtagct tctacatgtt cgcgtacaaa atcatacgat tttgtagagc
     8101 ctacattgtt gttgaaagtt acacatggtg acataacatt aattaatgca aatccttgat
     8161 gagacattgc tgcttgtatt aatggaatta attgtgtttt atcaccgcta aaactctgcg
     8221 ccacaaatgt ggcacctagc tcgattgcca aactacacaa atctattcct tcaaacatat
     8281 tggtatttcc tgatttgctt ttcgaaccta catcagcagt ggcagaatct tgtccttttg
     8341 ttaaaccgta acagccatta ttcataacaa tgtaagtcat gtttagattt cttcgaatga
     8401 cgtgaacaaa ttgtcccatg ccgatagatg ctgaatcgcc atcgccagaa acaccaagat
     8461 aaattaagtc tttgtttgcg aggttggcac ctgtggcaat cgacggcatt ctaccatgaa
     8521 ctgaattaaa accatgtgaa ttgtttaaat aatatgcagg tgtttttgat gaacagccaa
     8581 tgccagataa tttagccact ttatgtggtt caatgttcat ttgccaacat gcttcaacga
     8641 ttgcggcgcc aattgaatca tggccacagc cagcacataa agtactgagc gcaccttcat
     8701 aatattttct ggtataacct aatttatttt taggcaaatg aggatgtgca aactgtgatt
     8761 ttatataggt catacactta gtgtttcttt gttgtttaat aatgattttg gcaattgtgc
     8821 aatcgtctgc ttaacgataa aatcagcggt gataggcatg ccatcaaagt ttaaaatctt
     8881 tttcaacttc gatggtgcga tttccagttc atttatcaag agcgtgcgca tttgtgcatc
     8941 tctgttttgt tcaatgacaa acacttcgtt gtgtgcatta ataaaattga gtacatcgtc
     9001 tataaatgga aatgctttga tacgaatagc atctatgcta attccttgcg tatttaatat
     9061 atccatcgct tcttcggcag cagcagtagt cgtgccatag aatataaagc caatttcatg
     9121 ttgattgtct ttttggtaaa agattggtgc tggcaccatt gattttgccg tttcaaattt
     9181 ctgcaataat cgctccatga tgcgcacata agttgctgag tcttctgaat atactgcata
     9241 ttcatcatgc gatgaaccac gtgtaaaata ggcacctttg gtagcatgcg ttccaggaat
     9301 tgttctgtat ggtacgccat ctttatcgat atctaaatat ctaccgaagc gttctatgtt
     9361 ttctaattgt tcagccgata gcacttttcc tctattatat tttctatctg gatgccattg
     9421 taatggtgca gaaatatgat cgttcatgcc taagtctaaa tccagcatga ccattactgg
     9481 tgtttgtagg aattctgcta gatcaaatgc tgttgctgtc atttcaaaac actcagctgg
     9541 tgttgacgga attaataata catgcttagt gtcgccatgt gatgcataag ctgctaataa
     9601 aatatcagac tgctgcgtgc gtgttggcat gcctgttgaa ggtcctgaac gctgcacatc
     9661 tactaatact actggcactt ccgagtaata tgctaagcct aaaaactcat tcattaatga
     9721 aagtcctggc ccacttgtag cggtgaaaga acgtgcgcca ttccaattgg caccgatagc
     9781 catgcctaag gctgctaact catcttcagc ttgcacgatg gcataatttt tctttccagt
     9841 ttctttgtca tttctatata aaatggcata gtcttcaaaa gcttcaatca gagaagtaga
     9901 tggtgtgata ggataccaag aaactactga tgcgccaccg taaactgcgc caatgccagc
     9961 agcagtattt ccatttagta atattttatc tttaattaaa tttcttcgtt ccacctgtat
    10021 atctaatgga taagaaaaat tttcttttac atagttcaca cctaattgca atgcatgaat
    10081 attgggtgct attaattttt cttttccttt aaattgttca gtaactaatg tttctaatac
    10141 ttcaaattca atagctaaaa gtgctgataa agcaccaaca taaataatat ttttaaaaag
    10201 ttgtcgttgg cgcggatcgc tatattcttt attgcaaatg gccgttaatg gtatgccaat
    10261 aaatgtaata tcatcacggt gatattcttt atgcaatggt ttagtattgt catacataaa
    10321 gtagccacca ctttttacac tttgcacatc tttgagcatg ctttgcggat ttacgcatag
    10381 cattaaatca atgccttcgc gtcttcctaa gtaacctttt tcactcacac ggacttcata
    10441 ccaagttggc aatccttgaa tgttagaagg aaaaatgttt tttggagtta ccggaattcc
    10501 cattctaaaa attgacttgg cgaacagctg gttggcactt gccgatcctg tgccattcac
    10561 attggcaaat cgcactacaa aattatttac tttacttaat gacatgaacc tgctttactt
    10621 gttttatata aaaattcttg catatcccat gctgacgttg gacaacgctc agcgcacaag
    10681 ccacagtgca aacaaacatc ttcatctttc accattattt ttcctgtagt taatgttgaa
    10741 gacaccaata aactttgatc gagattgagt gctggtgctt ttaacttttc ccgtaatttt
    10801 tcttcctcat cattaaatgt aaatgtgatg caattggtag gacaaatatc catgcaggca
    10861 tcacattcga tgcattttgt tgcggtaaaa acggtttgca catcacagtt caaacaacgc
    10921 tgcgcttctt taaagcccgt aggtaagtta aagcctaatt caacttccat ttttcggttt
    10981 tgcaaaacta cagcttttgc tgcctgtggc acgatgtatc tatcatcatt agaaactata
    11041 ctgtcgtatg cccattcatg aatgcccatt tttgtactta ctaatgttgt aaatggtgct
    11101 ggtctatctt gtacattttt attttgacaa aataaatcaa tcgatatggc tgcctgatgg
    11161 ccttgtgcaa ccgcagtgat aacattttta ggccctaatg ctgcatcgcc accaaaaaat
    11221 acttttggta aagtagattg aaaagtattg ctgtcaacaa ttggtaaatt ccattcatta
    11281 aattcaattc ctaaatttct ttcaatccaa gggaaactat tttcttgtcc aatggcaatt
    11341 aatacttcat ctgcttcata aaatgcttct tcaccagttg atgttaaagt gcgtttccca
    11401 tttgtgtcat agctagcttt tactatgtcg aaagtcattc ctatgagttg agcatctttt
    11461 ataacaaatg atttgggaac atggttttcg atgataggaa tatcttcatg cacggcatct
    11521 tcaatttccc aaggacttgc tttcatttcg ctacgaggac ttcgcacgat tacttttacc
    11581 gcttcaccac caaggcgtcg tgctgtgcga cagcaatcca ttgctgtatt tccaccgcct
    11641 aatacgatta ctttttttcc aattttatgt gtatgctcaa aggcaacatt ggccagccaa
    11701 tcaataccga tgtgtatttt ctcttttgct tcttgtctgc caggcaagtt aagatttttg
    11761 cctctgggtg caccagtgcc aataaaaata gcatcgtaat cttgattgat aatttcggct
    11821 aagctattga cataagtatt aaaatgtgtg tgtatgccgt attgtaaaat ctgattgact
    11881 tcttcatcta acacttcatc aggcaaacga aaagatggga tttcactgcg catcatacca
    11941 ccagcagcat tgtggtcatc atagagatgt aattcgtagc caagtagcgc taagtcacga
    12001 gcgacagtta aagaagcagg accgccacca atgagtgcta tttttttacc attagctgtt
    12061 tcaggaattg gcaataaatg ttggatattt cctttgttgt ctgctgccac gcgcttaagc
    12121 cgacaaatgg caactggttc ttcatctgct aatctgccac gacggcaggc aggctcgcaa
    12181 ggtcgatcgc aagttctccc taaaacacca ggaaacacat tggattccca attgactaaa
    12241 taggcttcat catatttctc ttgtgctata agtcttatgt attgcggcac aggtgtgtgc
    12301 gctgggcaag catgttgaca gtctactact ttatggtaat attctgggtc atttacattt
    12361 gtaggttcca agatgttaaa ttgtttctgt tacggcaatg ttacctatat tgcatttatt
    12421 tgctactata acaaatataa cgtgcaataa agtttccaac aagcaaaatt ataaacaatt
    12481 tggtaatgat ttgatgattt tcgtcagtat tttgactgcg atgcattttt agattgtgcg
    12541 atatgtattt attattttta taatataata aatatgtatt gtatacaggc atcaaaatca
    12601 tgttttatct ttttgaagat ataagtttga aaataatttt cttttgttgc tgtatcgaag
    12661 aaaggatctg tcaaaattaa gacgaagcaa agtgtaacct ctctatttat aattattata
    12721 cagattgcta tttttaatta actcaataac aacatcttct atacattaag caaaaaaaag
    12781 attactaaaa taagcgtaac tttgcgccca aataaaaata tatggaagga tttagagcgt
    12841 taggattaag tgatgaaatt ttagacgcat tagaaaagaa aggctatgaa tcgcctacac
    12901 caatacaagc acaaacaata ccattattat taaatggaaa tttagatgtg gtcggtcaag
    12961 cacaaacggg cactggaaag accgcagcat ttggtttgcc tattttggac cgatttgacc
    13021 cagcacaaaa aagcgtgcaa gctttagtat tagcaccaac acgagagcta gctattcaag
    13081 tagcagaaga aattaagagt tttaaaggcg ctaagcgaat ttggattacg acagtatatg
    13141 gtggtgcacc aatcggagca cagatgcgcg aattggagaa aggcacgcat gtagtagtag
    13201 gtacgccagg tcgcgtgatt gatttaatcg aaagaaaaaa attagtactc gacgaagtaa
    13261 aatacgtagt attagacgaa gctgatgaaa tgttgaacat gggtttcatt gatgatatcg
    13321 aattgatttt gaaatcatgt aatgaaacgc gtagaatgtt gttgttttct gcaacgatgc
    13381 ctgatagaat tctctcatta gcgaagcgct acatgggaaa atttgaaatg gtgcgtattg
    13441 ccaaacaaga aattacgaat gtaaatatcg agcaatctta ttttgaagta ccagacaaag
    13501 accgttttga tgctttatgt cgcgtattag atgtggaaga agatttttat ggtatcatat
    13561 tttgtaatac caaggcggaa gtagtggaaa tatcgaacca cttaaatgac aaaggatata
    13621 cttcagatgc tttgcatggc gacatacaac agaatttgcg agagaaagta gtaaagcaat
    13681 ttaagaccaa taaattcggt gttttagtag caacagatgt ggctgcacga ggtatagata
    13741 tttctgattt gacacatgta atcaattatg gtttgccaca agatcctgag tcttatgtgc
    13801 atagaattgg ccgaactggc cgcgcaggca aagaaggaaa agctattgct attatttcac
    13861 catcagaaaa acgaaaatta ctttttgtag agcgtattgc aaaaacaaaa atgaagaaag
    13921 gtaaattgcc tgatgctaat gatatggtga aaattaaaac acgtcgttta acagaacacg
    13981 tggaaaccat tattggcaca gagcattata gcgaatattt agagctggct gcacaattga
    14041 ttggcgataa cgaagtagaa gtaattgtag ctgctttaat caaacatttt gcaaaagatg
    14101 agttgaaccc gaaagcctac aaacaaataa aagaagcggc accacgcgaa gaatttggca
    14161 atcgctttga aaaaggcaga agagatgata gaggtggcag aagagacgac agaagcagca
    14221 gaagagatga tagaaatgac agacacgaca gacgtgatga gcgtggtggc aatcgtcgtg
    14281 gtggttctgc gcaacgattg ttttttgcaa aaggcagagc agacggcatg acgccgcgtg
    14341 cgctattaga cgaattaaaa aacactgcag gtatcgaatc tcgtagaatt gatgatatca
    14401 gaatattaga taatttctct ttctttgcaa ctaacaaaga agatgcggaa caaattttag
    14461 ctgtatataa acgcaataaa ggcaatggaa aaccaatggt ggacaaagcc cgcgagcgcg
    14521 atgatagata gtatttaaaa aacgaagcct ttcggcttcg cttacaaaaa atgaataata
    14581 ccacttgatg aaaattcaag tggtattttc ttttagtgtt atagctttat gatatatcca
    14641 cctggcgcat ctacttctcc atcgatatca aattcttgtt ctgatgtgcc gtcatttatt
    14701 tttaccgtaa cagatgctgg cggattgctg ccttcattat ctacattaat accaatccaa
    14761 ttgtcgccat tttctaagga tacagtaaaa catctttcag tatttaaaat ctcatagtca
    14821 ctaatgagtg tgttgccatt aaatagtaaa tctatgatat cgccatcttc tacgctattg
    14881 tcgtaaatgc agattgaaat atttttattt accacattta tcgttccttg attggtgtaa
    14941 ttagaaggca attgtgtttg tatttttatt ttgtcttttt tgcaaccagt gttgattgat
    15001 ataacaaata acaataatag tattggaata aattgtttga tttttgtttt catagtttta
    15061 ttttttaagt gaatgataat ttatactact gtcaaaagtg ccatttttaa agtcgtaata
    15121 aacgccgata aaaccccaat tgtatttcaa gctgtaatga cgtcctgcta tattcatatc
    15181 ccaaataaaa ttacttttgt cgtccatttg ctctgctatc cattttgttt ctaaatcacg
    15241 ttgatttgac catggaactc gttgtatcgg tttggtttcg atttcaaatc ctattttata
    15301 tattttttct tctataaaat aaaaccaaaa tctaatataa tattcgtcgt atttggcttg
    15361 ttttaaagaa caaatattgt cagcgtattc actttttttt agttcatttg atgtaaaagt
    15421 gtttagcaaa ttttctaatg ttgtgtcttt gctgataata aatccattgt ataaagtgag
    15481 tgtgccagtt tctttatcga tgattttttt tgacataaat attaaatatc taaaacgaga
    15541 ctttggaatt agagaagatt aattttttaa atcagcaaga caatttttgt aattttccca
    15601 tgcagcatct actccgccag aaaaagctaa aggcattttt ggtaacaatt taaaagcatc
    15661 attaatacgc atcataaaat catcttgact caaataagtg tagatgaaaa acctacgttt
    15721 ttcttggtcg gtaacatgaa atgctataac ggcggcaaga tctttttcaa tcgtatcggt
    15781 tagacatgct tccatttcag tcaataaagc agtttcgttt gcatcttgtc caatagtgtt
    15841 tgatctaaaa tctacaaaca ctacttgatt atatttaccg gtttctttga tattttgtaa
    15901 gtttggtcgg atacttattt ttatattgtt atttggagta aaagaaatcc attcgtcact
    15961 gagaagaatg ccatttacat tatatgttgt aggaatttct tcgtccttaa ttgtcaaaat
    16021 agttttatac ataaattgca tttttcgttt caatagattg tagtattttc tattaatatc
    16081 aaacactact tcaatgctat tgcaattcat ccttgttttt ccttcttcat caaaagagaa
    16141 aataatattt ttactgctta attctacttt agaaacgcca ccatataggc tatgtgattg
    16201 gtcgttgata gaaatatgaa ctcttccatt aatgtaattt ttttcaaaat catcttttgc
    16261 ctcaaatccg agttgtagta taatatgttg cttactggca ttattagtaa attctatcat
    16321 ctgtacatca tattcgctaa tgtaaatttt tacattgctt gcaataaagt ttgctggaat
    16381 tggaattatt ggtttcgcat tggtcattgc tgcagagaat gccattgccg tttttcccat
    16441 atccattggg tcgctcattt ttgctgtata atatgtatgt gcttcttgcc attgtgcaat
    16501 tgtaatgccg ttgctactac aaatttgttg ttcttgttca ttgtttccag ttgcagctat
    16561 catcgataaa taaagtgctg catattgttc taaagctaac atagttttgt ttttgtattt
    16621 ttagttatta taaatagtta attcaccaga aactgttgca ggtgcatcta gtcctccttt
    16681 gttatacatc atacttggct gtaatgtaag cttggaaaaa gtgcatttaa tttcatttcc
    16741 atttacaaca aaatatagct taccagtttt gttgttgctt tcccacaaca tttggtctgt
    16801 gcttctcatg tcagcataac taattgagat atttgttgca tttttatcct ctacattgta
    16861 ctttacaatg gtataagaag atgagttttc aggaagcttg tctgtattaa aattgatggt
    16921 aaatgatttt gcaggtatgc tatcgtcagt aacagcaatt gttagtgttc ccatagagga
    16981 tactgctccg caaacgccat ttatttcata ggttttatca atgcaattta aggtgatttt
    17041 tccttttcct gctttgcctt gttctgttgt agatgatttt atttcatcga tattttgctc
    17101 aatagttgtt tcagaactat ctggcttgtt ggttttgcat gcagtgaaaa acacactgca
    17161 tacaaaaata taactccata gttttttcat agctatatct tagaaattaa tactagcatt
    17221 gtttattaaa ggatgttttg agccaccacc agtttggtct aatgcaagat tgttaatcgt
    17281 aattttttta ccggaaacag aaatggtttg actgccatct gggtcaaatg ttaagccatc
    17341 gacgccaaca ttaacgctac cacttggcac attcaaagca tcgtcttcta catcaaaagt
    17401 gccgccagct tcagcttctg cttgattgtg aaaagtaact tgtatcaact taaaaggtgc
    17461 gtcttgctgg catacaatag aataattgtt attgacaaaa gtttgcactt ctgtgttgcc
    17521 agtatagctt acaccatcaa tggtgaaagt gcctgttttc cctgcgctgt tcttgtcttt
    17581 tttacaagct gttgttgtgg ctactgctaa aaaaaggaat agcacgattt gaattgtttg
    17641 aattgttgtt ttcatttttt tgtaattttt tttgtaatta ataattatga caacttaatg
    17701 tattatcatt tataaatccc aacctttaaa taaattgctt agtggctgcg aagtaattgt
    17761 cattagggtt tgtctgcctt tgtcagctcc attaacaaca tcatctacag cattccgcaa
    17821 gaaataggca tacgttttgg tatccatatc tacaaatact aatgcagtta cgtttttacc
    17881 attataagtt agagtctgca tactatgatt gtacgaaagt gcaataacaa aatcagcttt
    17941 tgtccaatca attgggtcag tatccatatt gccattaggc tttttaacta aatcagttat
    18001 tttataacca gtataaattc ctcctgaatt atttatatta taatcgaaaa gatactcttg
    18061 tgttttaaaa ttaaagtaga atgcatttct tgttgaaaga attgaattat aattttcata
    18121 aacttcagca caaccattta taataccagc atttccccag ttgggctttg aggcaaaatc
    18181 atcaaaaaag tttgtgtacg aatatatttc tgtagaatcc tcataatctt gataggctgg
    18241 ataaagctga ttggtattgt cattattcat aataagccaa tcgcctactg ttgaaatgtt
    18301 tttccaagct ggccaatttt tatctgccaa atacatttct ttattatgat taatcgataa
    18361 atcaccataa tttgctttta tagttttgct gccaattggt ctgcttttta catattgcaa
    18421 ataaaaatta tcagctgttc cgatgatatt aaaagcagct tttttaggat tattgacatc
    18481 tcttaaatca aaaaattcaa tttctccatc tttttccata cttaatgatg gtgcaatttt
    18541 cccttttaaa tttactgcag gctctggtgt attttcattt ttacatgctg taagtgtgct
    18601 taatgatgct actatcatta aacttaaaat ttttgttttc atttttttca ttgtttataa
    18661 ttatggaata ttatttacta aaatcgaatg gcaattcatt tcccacatta gaaccagctt
    18721 cattaaaaaa tgcctcgttg aaattgataa ctaatcccgt gtcaaaagtg aatgaaggat
    18781 tattgctgtt tttattgatt cgtgtgatga tgtaaaattt tttagcatca aaatcaaaga
    18841 aataataaag gtcttcagct gtgctatagt ttactgtaca tgtagccgca tctatatttt
    18901 tccaatctat agagttacaa gatggacaaa gaatagttaa tgcattttct aaaattaaat
    18961 cagcgccagc tctaaatcca taatacaagt atttttgatt tgagaaatca taaaaaatat
    19021 aagaatcatt ttttccatta ttgtcataca cttgttcgta tgaataggta gcagcactaa
    19081 atttacttac atcagcatat tggctgggca tatcaggaaa ttcataacta aatgtattaa
    19141 acgttttgaa ataaatcggc tcacctaata accatttaat atttcctgga tcatacactt
    19201 tgttatcagg agtaaagaga cgaacattgg gcgtcataaa tgcattttca ttttctatag
    19261 gtagctgata agcattaggc gttactattt tctttaaata aaatttagtg taatcatctg
    19321 gttccatttg cacaatgcgc cattgatttc cttttttttg ataaaaatct atgcttaaca
    19381 atgcgccact attgtctttg gtgatattaa ccattttggt aaattgccaa gtttcagaag
    19441 cagtttcttc ttttttacaa gaactgaaga aaaagattag taatactata ctaatacgat
    19501 gttttaaatt tttcataatt cattttttgt gatgtaaaac tgcatcaaac agaaaatgaa
    19561 ataaaatgaa gtattttagc tttgtgagtg ttgtttatgg attttatttt gtaatatagt
    19621 tgttatcagg aagttagatg tatttgtttt tttaagttat tagaccaaat tttgttcaat
    19681 ttcttgcttt aaccacaaat aaacagaaac taatttgaat tcagtcggtg ttttttcttc
    19741 aaattcaatg atggaatcct gtaatatttt cacatctttt ttcgataatt ttttttgcaa
    19801 tacatgcttc tgttttgctg tataaagttt tttgtataac aatgaaattg ttttgtgatg
    19861 tgacatttta tctgaagcca cagattgtat aacgtttgaa ataatagaaa gtgctgtgtc
    19921 aaaatcttgt atcaaataaa agtagatgga tttgatagtt ttcaatacat agttttgcgg
    19981 gaaaggt
//
LOCUS       DAOQQV010000038        19649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_830_length_19649_cov_7.934317, whole genome shotgun sequence.
ACCESSION   DAOQQV010000038 DAOQQV010000000
VERSION     DAOQQV010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19649
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_830_length_19649_cov_7.934317"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLL59_05690"
     CDS             <1..355
                     /locus_tag="PLL59_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HMY23332.1"
                     /translation="SDSKKSNTLLDDYGNILNNSGRLVSPASTIVFDTWEHYVFVKSA
                     TGNFMKIYKNGIEIATKTGGAAISNKAKKVVLGGSEANDEHNDQLFKGSLDDLKIFNR
                     ALSISEVSNIYNTEM"
     gene            538..1287
                     /locus_tag="PLL59_05695"
     CDS             538..1287
                     /locus_tag="PLL59_05695"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY23333.1"
                     /translation="MKEHHPHLDLPETSLKHRKIVFSKPFLKRIHEDWYLAFKNYAQA
                     SPLQGEIIEIGAGGGFLKDIYPEVITSDIMPLSVCDMQFSATAMPFENNTLKAIFMLN
                     VLHHIPDNNAFFTEANRVLKSGGFIYMIEPATTWFSKFIYKNFHHEPFDENVEEWKFA
                     SKGPLSDANGALPYIIFKRDKTTFYKLYPELEVIEFKYHTPFKYLLSGGLSKPAIVPY
                     FIYGLVCFIEKIFSPLFPTLAMFQTIIVRKI"
     gene            1293..2726
                     /locus_tag="PLL59_05700"
     CDS             1293..2726
                     /locus_tag="PLL59_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HMY23334.1"
                     /translation="MSSSDRKQEIYNHFENAANNYLQYRKRFSYYGNDIINYLNFFIS
                     ETDSILEVGCGCGETIAHLKGNRKVGIDFSPKMIEVAQQNFPDVDFQVMDAENISLDK
                     QYDVIIFNNIIGYLDNIQDVFYAIKKNCHEHTRIIISYYNHYWEPILNFGETIGYKKK
                     SPQQNWLDAKDISNLLYLSGFETYRINKRLLLPIYIPILSWLLNKYVAKLPLFKSMCL
                     NQFTFSRPLLNHSETVVADKYSVSICIPARNESGNIENAILRLPKFGKHQEIIFVEGN
                     SSDDTWQKIQEIQEKYKHTHDIKIAQQSGKGKGDAVRKGYSIATQDILMILDADLTMP
                     PEELPKFYNAIATGKGEFINGSRLVYPMDKEAMRFLNTLGNKFFSWVFSWLLEQPIKD
                     SLCGTKVMFREDYLKLIANRKFFGDFDPFGDFDLLFGAYKLNLKIIDLPIRYKERTYG
                     TTNISRFTNGFMLLRMCWFAAGKIKFW"
     gene            complement(2774..3682)
                     /locus_tag="PLL59_05705"
     CDS             complement(2774..3682)
                     /locus_tag="PLL59_05705"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMY23335.1"
                     /translation="MRKRISRAPIQPLTSPNKTNFHKNKKSFVPKNDPFAYKIEQTVK
                     NFTPPKDEPAPDEMRLNKFISNAGVCSRRQADEYIKAGYVTVNDVAVLEMGYKVKPSD
                     IVKFKNRKVQNEKKVYVLLNKPKGYLTTVSDDRERKTVMELVSTVKDVRLYPVGRLDR
                     NSTGILLMTNDGELAQKLSHPSGEVTKVYQVELDKPLDQKDLLKIREGVELEDGKANV
                     DQVEYTLPRGDDKHVGIELHSGKNRIVRRIFESIGYEVQKLDRVLYAGLTKKNLPRGK
                     WRFLTEKEVIFLKYFIANKKEKKKVE"
     gene            complement(3624..4226)
                     /gene="scpB"
                     /locus_tag="PLL59_05710"
     CDS             complement(3624..4226)
                     /gene="scpB"
                     /locus_tag="PLL59_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HMY23336.1"
                     /translation="MEEVQKNIEAIIFAAESPVKTEELLTYLNKTAETTFTQEEIDGF
                     IFVIKEKYNNDLFPFELGEIGGGFQFLTKADYHVAVSTFLNRNTVKRLTTAALESLAI
                     IAYKQPVTKNIIETIRGVNADYTIQKLMEKELVEIAGRSEEPGKPLLYKTTQQFLDYF
                     GINSIDELPKLKEFEELQNQIGEKENIESANSAADIAQQN"
     gene            4396..5037
                     /locus_tag="PLL59_05715"
     CDS             4396..5037
                     /locus_tag="PLL59_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HMY23337.1"
                     /translation="MQHLIFDLDGTISNPETGILNGYKYTFPKIGLATPDDATLRTLI
                     GPPLRIVFENMYGLSTDTANFAINTYRTYYNELGGAYENELYNGMKTLLETLHVQNRK
                     LHIATHKGAMTEQILQHFQIRNYFSQIQHYNEEKDITTKEKMIALIMQAENISDKNNV
                     LMIGDRHSDIEAAHYHQIGSIGVVYGFGTLAEIQLSKPTHIADDVQKLAQILL"
     gene            complement(5050..5250)
                     /locus_tag="PLL59_05720"
     CDS             complement(5050..5250)
                     /locus_tag="PLL59_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23338.1"
                     /translation="MKSLVWLMFSILIISACQEEEGGRKDNSKCTCKNSDHVVVGTYT
                     GLTYEECMEKSRPDLGETCILE"
     gene            5407..6567
                     /gene="nhaA"
                     /locus_tag="PLL59_05725"
     CDS             5407..6567
                     /gene="nhaA"
                     /locus_tag="PLL59_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HMY23339.1"
                     /translation="MLKKITTPFIHPIRELAESGKLGGVLLIIATLFSIIVTNSKYGE
                     AYLHFWHINIGNSFLSMHIEHWINDALMAIFFFLVGLEIKREIVSGELSSPKKAMLPL
                     LAAIGGMIFPALIYFLFNNNTHNAHGWAIPTATDIAFSLGVLSLLGKRVPLSLKIFLT
                     ALAIIDDLGGIIIIAVFYTAEIHFTYLLYAGIILFVLFLLNTFKVRKFIFYFIPALCL
                     WYCVYQSGVHATIAGVLSALFIPIEKIEKYEHYLHQPVNYLILPIFALVNTTILLSAD
                     SIVHVSSPLGLGIILALFLGKSFGITLFTYISIKTKTGELQDNSDIKDIFGVSMLAGI
                     GFTMSLFFTALSFDNPESANIARLSIIIGSTLSAVCGLLFLSFTLDKEAMNQ"
     gene            6834..7997
                     /locus_tag="PLL59_05730"
     CDS             6834..7997
                     /locus_tag="PLL59_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMY23340.1"
                     /translation="MTEAAFLHYDLKLIEPSFDSELTDLIIELDYLRKKPLGGSTHPK
                     VFFQLKHIFHTLESIGSARIEGNNTTIAEYIETKLAVNNKIPPNIKEIQNIEKAMAFI
                     EENVKDYPINRAFLSEMHKMIVDGLLPPPGGEGDGTPGEYRKTNLKINKSTHKPPEWL
                     KVDDYMNELLDFINREDSPKYDLLKAAIAHHRFVWIHPFGNGNGRTVRLFTYAMLVRT
                     GFNVNVGRIINPTAVFCSNRNDYYTNLSEADKGTDAGIITWIEYVLKGLKEEIEKIDK
                     LLDYKFLRKEILIPTITHSLERKYITDVEAKILKKAIDEQVIQAADVKEFFGGKADAE
                     VSRQIRKLIDKKMLVPEKEGTRKYVLRFDNNYLLRSVIKTLGEKGFLPVRDEV"
     gene            complement(8230..9177)
                     /locus_tag="PLL59_05735"
     CDS             complement(8230..9177)
                     /locus_tag="PLL59_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23341.1"
                     /translation="MKQNEQSFNSKILLFGEYSIIQDSMGLTLPYDEFQGKLTFDAND
                     MNEQFVSESNEHLEEFANYLQRLLSTNELAIQFDIQAFKSDIAKGMYFQSSIPQGFGV
                     GSSGALVAAVYGKYTKDKVLVDAAPNKSDILQLKAIFAKMESYFHGNSSGVDPLICYL
                     NLPLLINGKNGVEPVKVELSNKGKGAIFLIDTGISRSTEPLVKYYMQKMEEPSFREFV
                     DKQLKPFANNCIQAFLDGDRETLFAYMKLLSKFQFEHFKPMIPALFNKVWKRGLDSGE
                     YYLKLCGAGGGGFILGFTKNYELTKKQLKDQNIELLCSI"
     gene            complement(9199..10257)
                     /locus_tag="PLL59_05740"
     CDS             complement(9199..10257)
                     /locus_tag="PLL59_05740"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23342.1"
                     /translation="MNPFQNKQGETAWRTPSNIALVKYWGKYGQQLPRNASISFTLNN
                     AFTETVVKYYSKTKDDKNIDIQFMFEGKVNPKFAEKIHKFLESIVPHFPFLPYLTLEI
                     SSQNSFPHSTGIASSASSMAALALCLTTIEEQLNGDMLARSFLEKASMIARLGSGSAC
                     RSVYPYLAVWGQHDAIQDSSNEYAVPFYEQVHPTFKTFKDAILIVSSDEKSVSSRAGH
                     ALMEGNPFADSRYSQAKTHLTTITDCLKYGDLEKFGEIVENEALTLHALMMCSTPSYM
                     LMLPNTLKIIDLIKTFRTEKAVPLYYTLDAGPNVHLLYPAGNAVEVETFIQQALLPYC
                     EQQKVIFDEVGKGPIRLK"
     gene            complement(10335..11123)
                     /locus_tag="PLL59_05745"
     CDS             complement(10335..11123)
                     /locus_tag="PLL59_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMY23343.1"
                     /translation="MHKIFLSVVLMLCASTFLIAQDSIPPSVKSTLAGPKGISEKVKK
                     RITGANDRLVIELAMMNAIVKKDGGVTVPNDFKLGAFNRGINVYFMYDILLGKKTKPR
                     HFSIAPGIGVGSENYYFKNYKLTWHYDTLTRFQAFGDSISSKKSKLNMTYIDVPIEFR
                     YRSKPHAKTGMSWKIAVGFKLGFLVGSKWKYKGEDPNVPNNGETVTIKDMKVANMSKF
                     RYGPTLRAGYGPLSLFAYYSIGSAFTTKGPKMNPIVFGISINGL"
     gene            complement(11257..12864)
                     /locus_tag="PLL59_05750"
     CDS             complement(11257..12864)
                     /locus_tag="PLL59_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23344.1"
                     /translation="MILNYEKSLNAWDKASFHSAVKPYTAAEVYQFVFPDTVQHYERV
                     PHTNWFSKLVNVVGFENLLEFDEKGYVKHYGKILAKDSVFYEDNIYDKAYKKRKVYVS
                     LNPILNIGFGYDVSSKKFLTYNLKGLELRADIGKKVTLYTAFLNTVGKFPTYIDIYNA
                     QTGVVPGEGNVRNRGKQGLDFSSIYAYISYSPVKQFNIQVGNGKHFIGDGYRSLLLSD
                     NAYTYPYLQLTANVWRIKYTTIYAELIDNIRNFNDFGQGFPRKLANFNYLSIDAAKFL
                     QIGIFEGITWRRTTVNGNNYFNYNFLNPILGVRGFQKKLEPNTTKVYGLNVKVTCPKY
                     VVLYGQFMINKYGKKNTVDRRMGWQAGVKYFDVGGVKNLNFQMEYNSVRPFTYQGQDS
                     GIGYSHYNQSLAHPMGANFNELLLMANYQYKRFYTSYKMSFIKTAADSAVSQNYGNNI
                     LDIASQASNISGIKIQNGIPYKVVDHDLRFGYIINPKINMVIEAKLQFRKYTTNLAST
                     SSLKTNSFMVSFATNIFNRYYDLPVVY"
     gene            complement(12947..14362)
                     /locus_tag="PLL59_05755"
     CDS             complement(12947..14362)
                     /locus_tag="PLL59_05755"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HMY23345.1"
                     /translation="MKKRITNLYVLMHALGDLFILNTAYIIGHFLSDNYRMIFDTKYL
                     QLWIYLNVIYLFAAQLSGTFDMYRNTRFFTLLSALLRLFFFEVLFAFSYVVVFKDFNQ
                     TFKLSRELLLITYTVAFFFTMMWRFGFIKIVRFYRARGFNNRKVMIIGAGDTAQEFKA
                     MLEHKIEYGYQFLGFYDDHPTHFKPEIQKNILGNIEDAKQYAITHQIDELFCALPYKE
                     EEKIKSLIQFGDENLIRVKIVPDLSRFLTHHLNKVEIDYYGQIPVMTLRSEPLENIVN
                     RFIKRLFDLGFSILVFLFILWWFIPLVAFLIKITSKGPVFFVQQRSGIKNKSFQVIKF
                     RTMYINSDAHYKQATRDDQRITPIGKLLRRTNLDEMPQFINVFFGQMSVIGPRPHMLK
                     HTEEYSKIIDKFMVRHLVKPGITGWAQVNGYRGETKDVTDMEGRVRADVWYIENWSFG
                     LDVKIIIMTIINMIRGEENAF"
     gene            14758..15780
                     /gene="recA"
                     /locus_tag="PLL59_05760"
     CDS             14758..15780
                     /gene="recA"
                     /locus_tag="PLL59_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMY23346.1"
                     /translation="MSKEDKANKEAGSEKAKALLLTLEKLDKQYGKGTVMKLGDNSIV
                     ESEVISTGSLGLDLALGIGGIPKGRIIEIYGPESSGKTTLAIHAIAEAQKKGGICAII
                     DAEHAFDKKYAEALGVDTDNLYITQPDDGEQALDIAEQLIRSAAIDLVVVDSVAALVP
                     RSEIEGEMGDSKMGLHARLMSQAMRKLTGAINKTSCSCIFINQLREKIGVMFGNPETT
                     TGGNALKFYASIRLDIRRAGQIKDKEGNVIGNQTKVKVVKNKLAPPFKVAEFDIMFGR
                     GVSKEGEIVDMGAELGILQKSGAWYSYESQKIGQGRDQVKQLMLDNPGLADEIEGKIR
                     EKLKTV"
     gene            complement(15979..>16470)
                     /locus_tag="PLL59_05765"
     CDS             complement(15979..>16470)
                     /locus_tag="PLL59_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HMY23347.1"
                     /translation="TKDKVAFGLGRLEIRAKLNAAKGAWPAFWLLAQGRPYPQGGEID
                     IMERLNSDNFVYQTVHSTYTINLGIKDNPKNYGTAAINPNAYNTYAVEKYADKVVFYV
                     NGTKTFTYPKIQTTQPDQFPYADGDHYLLLDMQLGGSWVGTVDGNQLPVEMEIDWVKF
                     YKF"
     assembly_gap    16471..16570
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16780..18027
                     /locus_tag="PLL59_05770"
     CDS             16780..18027
                     /locus_tag="PLL59_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23348.1"
                     /translation="MEFLQTIISDFKAHWYIYLSMPIIAALIGYGTKIVAIKMMFEPL
                     EFFGIKPFLGWQGIIPRKAAVMSTIACDTLTARLIKPEEIFARLDPNRIAQELEKPML
                     PIVEKITHDVMSHYQPGLWESIPENVKKMLIKQIQQQSPDMVRQLMQQTKDNLNEVFD
                     LKDMVVTNLTKDKKLLNKIFLESGEKEFQFIRNSGIYFGFLIGLVQMVVWILTHNVWV
                     MPLFGLFTGWFTDWLALKMIFAPKEPKKYFGLFTWHGLFFKRQQEVASKYGALIAKEV
                     LTPANMIESILTGKMSDNLFNMIQKNVQQMVDEQAGILKPVVVFAVGSEKYKAMKIAI
                     TGNMVHQLPVALKHIEKYAEDAMDIQNTLVSKMQELTPEEFEGVLRPAFKQDEWILIT
                     VGAVLGFLVGELQVLIMEHFVLR"
     gene            18229..>19649
                     /locus_tag="PLL59_05775"
     CDS             18229..>19649
                     /locus_tag="PLL59_05775"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HMY23349.1"
                     /translation="MSNKVFIFDTTLRDGEQVPGCQLNTIEKIEVGLALEALGVDVLE
                     AGFPISSPGDFKSVVEITKNIFNPTICALTRAVAADIDCAAEALKHAKRGRIHTGIGS
                     SDIHIKNKLKTTREAILEKAAWAAKYARNYVDDVEFFCEDAGRADLEFLARMVETVIA
                     AGATVVNIPDTTGYCLPEQYGAKIAYLMNNVPNIDKAIISAHCHNDLGMATANSMAAL
                     QNGARQVEVTMNGIGERAGNTSLEEIAMILKSHENNLNLQCDINIKKIYPTSRLITRL
                     MRMPVQPNKAIVGRNAFAHSSGIHQDGVLKNRENYEIIDPHDVGIPESLIVLTARSGR
                     AALKHRLENIGFTLDQAQLDETYKKFLDMADQRKEIFDEDLLEMMGQSREGKIYEIDY
                     LEVHCGKRHQATATVALVKNGKRTVEIADGCGPVDATLKAIDKIVQQPVRLEEYLVQA
                     VTGGSDDTGKVHIQISKNGVFYYG"
BASE COUNT         6264 a         3449 c         3512 g         6324 t
ORIGIN      
        1 tagtgatagt aaaaaatcta atactttatt agatgattat ggtaatattt taaacaatag
       61 cggcaggctg gtttctcctg caagtacaat agtattcgac acttgggaac actacgtttt
      121 tgtaaaaagt gccacaggca attttatgaa aatatataaa aatggaatag aaatcgctac
      181 aaaaactggc ggtgctgcta tttcaaacaa agcgaaaaag gtagtgttag gtggtagtga
      241 agcaaatgat gaacataacg accaattgtt taaaggcagt ttagatgatt taaaaatatt
      301 caatagagca ttatccatat ccgaagtgtc aaatatttat aatactgaga tgtaatatca
      361 ttgagaaact atataatcaa aagaggctgt ctcaaaaggc gaggtagcct cttttttgtt
      421 cggattcgta tctgagaaaa ttcagaatgt taaaatctta gagttatttt tttgttttga
      481 gacaacctct ttttattaat agtacttaat ttttgttttt gtaattttgc aaaaccaatg
      541 aaagaacatc atccacatct tgatttacct gaaactagtt tgaaacatag aaagattgta
      601 ttttcaaaac catttctaaa aagaatacac gaagattggt atctagcttt taaaaattat
      661 gcacaagcat ctccattaca aggagaaata attgaaattg gtgcaggtgg tggttttcta
      721 aaagatattt atccggaagt tattacttcc gatatcatgc cgctttcagt ttgcgatatg
      781 cagttttccg ctaccgcaat gccatttgaa aacaatacat taaaagccat ttttatgctc
      841 aatgtattac atcatattcc ggataataat gcatttttta cagaagcaaa tagagtttta
      901 aaatccggtg gttttattta tatgatagaa cctgccacta cttggttctc aaaatttatt
      961 tataaaaatt ttcatcacga accatttgat gaaaatgttg aggaatggaa atttgccagt
     1021 aaaggtccat taagcgatgc aaatggcgct ttgccttata ttattttcaa aagagacaaa
     1081 acaacctttt ataaactata tcctgaatta gaagtaatag aatttaaata tcatacacca
     1141 tttaaatatt tattatcagg tggactttca aaacctgcaa tagtgccata ttttatttat
     1201 gggttggttt gctttatcga aaaaatattt tctccattat ttccaacatt agcaatgttt
     1261 caaactataa tagttagaaa aatataatca gtatgtcatc atcagataga aaacaagaaa
     1321 tatataatca ttttgaaaat gcggcgaaca actacctaca gtatcgtaag cgattttctt
     1381 attatggtaa tgacatcatc aattatctta atttttttat tagtgaaaca gattccatct
     1441 tagaagtagg ttgtggttgt ggtgaaacga ttgcacattt aaaaggcaac agaaaagtag
     1501 gtattgattt ctcaccaaaa atgattgaag tagcacaaca aaattttccc gatgtggact
     1561 ttcaggtgat ggatgccgaa aacattagct tagacaagca atacgacgtt attattttca
     1621 ataatattat cggctattta gacaatattc aagatgtttt ttatgccatt aaaaaaaatt
     1681 gtcacgaaca tacgcgtatt attatttcct attataatca ttattgggag cctattttaa
     1741 attttggcga gaccattggt tataaaaaga aatcgccaca acaaaactgg ttagatgcaa
     1801 aagatatttc taatttattg tatctgtcag gttttgaaac atatcgcatt aacaagcgac
     1861 tactactgcc tatttatatt cctatattat cgtggttgct caataaatac gttgccaaat
     1921 tgcccttatt taaatcgatg tgcctaaatc aatttacatt ttcacgtcca ttattaaacc
     1981 attccgaaac agtggttgcc gataaatatt cggtgagtat ttgcatacct gcgcgaaacg
     2041 aaagtggcaa tatcgaaaat gcaattttgc gattgccaaa atttggcaag caccaagaaa
     2101 tcatttttgt ggaaggaaat tcttctgatg atacttggca aaaaattcag gaaatacaag
     2161 aaaaatacaa acacacacac gacataaaaa ttgcacagca aagtggcaaa ggaaaaggcg
     2221 atgcggtacg taaaggttat tctatagcga cacaagatat tttgatgata ctcgatgccg
     2281 acttgacgat gccaccagaa gaattgccta agttttacaa tgcaattgct acaggcaaag
     2341 gcgagtttat taacggctcg cgattggtat atccaatgga caaagaagcc atgcgttttt
     2401 taaatacctt aggcaataaa tttttctcgt gggttttctc gtggctctta gagcaaccga
     2461 taaaagatag tttgtgtggc acaaaagtga tgttccgaga agattatttg aaattaattg
     2521 ccaatagaaa attctttggc gatttcgatc cgttcggcga ttttgattta ttatttggtg
     2581 cttacaaact caatttgaaa attatcgatt tgccaattag atataaagag cgtacgtatg
     2641 gcacgacgaa tatctcgcga tttactaatg gctttatgct attaagaatg tgctggtttg
     2701 cagctggcaa aatcaaattt tggtaatgct attatataaa catagcagaa taggtatgta
     2761 gcgcaataaa cttttactct actttctttt tttctttctt attggcaata aaatatttca
     2821 agaaaatgac ttctttttct gtcaagaaac gccattttcc acgaggtaaa tttttcttgg
     2881 tgagaccagc atataatact ctatcgagct tttgtacttc gtatccaatg ctttcaaaaa
     2941 tgcgacgcac aatcctgttt ttgccactat gtagttcgat gccgacgtgt ttatcgtcgc
     3001 cacgaggtaa tgtatattct acttggtcca cattggcttt gccatcttct agctctacac
     3061 cttcgcgaat ttttagtaaa tctttttggt cgagcggttt atctaattct acttgatata
     3121 cctttgtcac ttcgccgctt gggtgcgaaa gtttttgagc taattcgcca tcattggtca
     3181 tcaataaaat accggtggaa tttctgtcta atcgaccaac tggatacagg cgcacatctt
     3241 ttactgtact caccaattcc ataaccgttt tgcgctcgcg atcgtcggat acagttgtca
     3301 aatagccttt tggcttattg agcaacacat atactttctt ttcgttttgt actttccgat
     3361 ttttgaattt tacgatatcg ctcggcttta ctttatagcc catttccaaa actgcgacat
     3421 catttaccgt tacatagcca gctttaatat actcatctgc ttgtctgcgt gaacatacac
     3481 cagcattaga gataaacttg ttcaaacgca tctcatcagg cgctggctcg tcctttggag
     3541 gtgtaaagtt tttcaccgtt tgctctattt tataagcaaa aggatcattt ttgggcacaa
     3601 acgacttctt atttttgtgg aagttagttt tgttgggcga tgtcagcggc tgaattggcg
     3661 ctctcgatat tctctttctc acctatttgg ttttgtaatt cttcaaattc ttttaatttc
     3721 ggcaactcgt caatactatt gatgccaaag taatctaaaa actgctgagt ggttttgtaa
     3781 agtaatggtt tgccaggctc ttcgcttctg cctgctattt ccactagttc tttttccatc
     3841 agcttttgta tggtgtaatc tgcgtttacg cctcgaatgg tctcaattat atttttcgtt
     3901 acaggttgtt tgtaggcaat aattgctaag gattccaatg cagcagtagt caaacgcttt
     3961 actgtatttc tgtttaagaa tgtcgaaacg gcgacgtggt aatctgcttt tgtcaaaaac
     4021 tgaaagcctc caccgatttc gcctaattcg aagggaaata agtcgttgtt atatttttct
     4081 ttaataacaa aaataaaacc gtcaatctct tcttgtgtaa atgtcgtttc tgccgtttta
     4141 ttcaaatacg taaggagttc ttccgttttt acaggacttt ctgcagcaaa aatgatagct
     4201 tctatatttt tttgtacctc ttccacagca caaatatacg aaaatatgtt gcgctatgct
     4261 attgtcgact aaaagtaaaa gaattgccat tgttaaaaaa tgaaaaaaca attgctatcg
     4321 tgatttaaga ataacatatt ctctattttg gtgtaattca gttaaaacta tgtatatatg
     4381 tagtcgatta agcacatgca acaccttatt tttgacttag atggcactat ttctaatccg
     4441 gagaccggca ttttaaatgg ttataaatat acttttccta aaattggctt ggcaacgccc
     4501 gacgatgcca ctttgagaac actgatagga ccgcctttac gtattgtttt tgaaaatatg
     4561 tatggcctat ctactgacac tgctaatttt gccatcaaca cctatcgcac ttattacaat
     4621 gagctcggcg gcgcgtatga aaatgaattg tataatggca tgaaaacatt gctagaaacc
     4681 ttacatgttc aaaatagaaa actgcacatt gccacacata aaggcgcaat gacagagcaa
     4741 attttacagc attttcaaat caggaattac ttttcgcaaa ttcaacatta caacgaagaa
     4801 aaagatatca ctacaaaaga aaaaatgatt gcattaatca tgcaagccga aaatataagc
     4861 gataaaaata atgtgctaat gattggtgat agacatagcg atattgaggc ggctcattat
     4921 catcaaatag gcagtattgg tgttgtttat ggcttcggaa ctttagcaga aatacaacta
     4981 agcaaaccca cgcacatcgc tgatgatgtt caaaaattag cacaaattct actgtaagta
     5041 ttgactacat tactcaagta tgcaagtttc acctaaatct ggtctgcttt tttccatgca
     5101 ttcttcataa gttaaacctg tatatgtacc cactaccaca tgatctgaat ttttacaagt
     5161 acacttacta ttgtcttttc tgcctccttc ttcttcttgg caagcactga ttatcaaaat
     5221 gctaaacata agccatacta aacttttcat aataatggtt ttatcataca atttaaacat
     5281 ttttatgatg cttcgcttat gtttttgtgc gcattacttt taaatgaaat aaatatcact
     5341 tcaaatacaa ttgctttttg taaaatagcg cttagtaaac tacctttgaa aatctaaaac
     5401 gtagcaatgc tcaagaaaat tacaacacct ttcatacatc caattcgtga gctggcagaa
     5461 agcggtaaac ttggtggcgt gctactcatt atcgcgacac ttttttccat catcgtaacc
     5521 aatagtaaat acggcgaagc ttatttacat ttttggcata tcaatatcgg aaattctttt
     5581 ttgtcgatgc atatcgaaca ttggattaat gatgcattga tggctatttt tttcttttta
     5641 gttggcttag aaatcaaacg cgaaattgta tctggagaat tatcatctcc taaaaaagcc
     5701 atgctgcctt tgcttgctgc cattggtggt atgattttcc ctgctcttat ttatttctta
     5761 tttaacaaca acacgcacaa tgcgcatggt tgggcaattc caacggcaac tgacatcgca
     5821 ttttctttag gcgtgctgtc tttactcgga aaacgggtgc cactttcttt aaaaatattt
     5881 cttaccgctt tagctatcat cgacgattta ggtggcataa ttattattgc tgtcttttat
     5941 actgctgaga ttcattttac ttatttattg tatgctggaa taattttatt tgtgctgttt
     6001 cttttgaata cattcaaagt gagaaagttt attttctatt ttattcctgc cttgtgttta
     6061 tggtattgtg tttaccaatc aggtgtgcat gcaacaatag ctggtgtgct ttctgcatta
     6121 tttattccaa tagaaaaaat agaaaaatac gaacattatt tacaccaacc tgtcaactat
     6181 cttatcttac ctatttttgc attggttaat acgaccattc tattgtcggc cgatagtatt
     6241 gtgcatgtaa gctcgccatt aggtttgggc attattcttg ccttgttttt aggcaaatct
     6301 tttggcatca cattgtttac ttacatcagc ataaaaacca aaactggcga actgcaagac
     6361 aattcggaca tcaaagatat ttttggtgtg agtatgttgg ccggcattgg ctttaccatg
     6421 tccttatttt ttactgcatt atcatttgac aatccggaaa gtgccaatat tgctaggctt
     6481 tccattatca ttggttctac actatccgct gtttgtggct tattgttttt atcatttact
     6541 ttagataaag aagctatgaa ccagtagtac aaaagctgaa tttaacactc agccctgctt
     6601 ttgcaaatat gtttgttagc tacttttata ctgttcattc gaagcaaaat tgttaaatat
     6661 tagcacaagt atattacaat tgtaatgcac ttgtgctaaa aatgtctgca aaacatttgt
     6721 taattgtagt aaaagaatgt aagtttgtaa cgtcttgata tacaagtcta ttttaatcca
     6781 agatgttaaa tcttgaatta tggttacgca aatcattaaa ttacttttct caaatgaccg
     6841 aagcagcgtt tttacattac gatttaaaat tgatagagcc gtcttttgat tctgaactaa
     6901 cggatttaat tattgaattg gattatctcc gaaagaaacc acttggtggt tcaacgcatc
     6961 caaaagtttt ttttcaattg aaacacatct ttcatacact tgaaagtata gggtcggcaa
     7021 gaatagaagg gaacaataca actattgcag aatatattga aacaaagctt gcggtaaaca
     7081 ataaaattcc tccaaatatt aaggaaattc agaatattga aaaggcaatg gcattcattg
     7141 aagagaatgt aaaagattat ccaatcaaca gagcattttt aagtgagatg cacaaaatga
     7201 ttgtagatgg attattaccg cctccaggtg gagaaggaga cggaactcca ggtgaataca
     7261 gaaaaacaaa tttgaaaata aataagtcaa cacacaagcc acctgaatgg ttaaaggttg
     7321 atgattacat gaatgaactc ttagatttta tcaatcgtga agacagccca aaatatgact
     7381 tgcttaaagc tgctattgca catcatcgat ttgtttggat tcacccattt ggcaatggca
     7441 atggaagaac ggttcgtctg tttacctatg ccatgcttgt tagaacagga ttcaatgtta
     7501 atgttggaag aataataaat cctactgctg tgttttgcag caatagaaat gattattaca
     7561 caaatttgtc tgaagctgac aaaggaacag atgcaggaat aataacttgg attgaatatg
     7621 tattgaaagg attgaaagaa gagattgaaa aaatagataa gcttttagat tacaagtttt
     7681 tgagaaaaga aattctcatt cctacaatca ctcactcatt ggaaaggaaa tatattactg
     7741 atgttgaagc aaaaatattg aaaaaagcca ttgatgagca agtaatccaa gcagcagatg
     7801 tcaaagagtt ttttggtggt aaagccgatg cagaagtgtc aaggcaaatc agaaaattga
     7861 ttgacaagaa aatgctcgtc cctgaaaaag aaggcacgag aaaatatgtt ttgcgtttcg
     7921 acaacaacta tctactgaga agtgtcataa agacattagg tgaaaaagga ttccttcccg
     7981 tccgtgatga agtttgatag tggcactgtc ggtcggagtc gcgaaatgct tgcaggtaac
     8041 gtcttgggat attggttgtg gtggaggatt tttagtacaa aagttcaaac gaagacgttt
     8101 acgcactctt gcgttcaaac cgcttattgt gggctgtttt tcttttttca gagtcgtagt
     8161 tcatctgttt taacttcgac atcagcttat gtataactgt tgacgcattg acaagaaaat
     8221 ggaaatgatt tatatactac aaagcaactc aatattttga tctttgagtt gttttttggt
     8281 gagttcgtag tttttggtaa aacctaagat aaagccgcca ccgccagcac cgcagagttt
     8341 gagatagtat tcgccgctgt cgagtcctcg cttccatact ttattaaata atgcaggtat
     8401 catgggttta aaatgctcga attgaaattt agatagcaat ttcatataag caaacaaagt
     8461 ttctctgtcg ccatctaaaa atgcttgaat gcagttattg gcaaatggtt ttaattgctt
     8521 gtccacaaat tctcggaagc ttggttcttc cattttttgc atgtagtatt ttaccaatgg
     8581 ttctgtgctt ctgctaatgc ctgtatcaat aagaaaaatg gcacctttgc ctttgttgct
     8641 cagttctact tttacaggtt ctacgccatt ttttccatta attaataagg gtaaatttaa
     8701 gtagcatatt aaaggatcga ctccagaact attgccatgg aaataagatt ccattttggc
     8761 aaatatggct ttcaactgca atatatctga cttattgggt gcagcatcta ccagcacttt
     8821 gtctttcgtg tatttgccat acactgctgc aactaaagcg ccgctactgc caacgccaaa
     8881 tccttgagga atcgacgatt gaaaatacat tcctttagca atgtcacttt tgaatgcttg
     8941 tatatcaaac tggatagcta actcatttgt ggacagtaag cgttgtagat agttggcaaa
     9001 ctcttctaaa tgctcatttg attctgagac aaattgctca ttcatgtcgt tagcatcgaa
     9061 agtgagcttt ccttgaaatt catcatacgg caaagtcaag cccatagagt cttgaatgat
     9121 gctatattcg ccaaagagca aaatttttga attaaaagat tgttcgtttt gtttcatatg
     9181 ctaattccaa atggaatatt actttaatcg tattggacct ttaccaactt cgtcaaaaat
     9241 tactttctgt tgctcacaat aaggtaacaa tgcttgctga ataaaagttt ctacttctac
     9301 tgcatttcct gctggataca acaagtgtac gtttggtcct gcgtctaaag tataatacaa
     9361 aggtacagct ttttccgttc ggaaggtttt tattaaatca ataattttca aggtattagg
     9421 tagcattaac atgtatgatg gtgtgctaca catcattaac gcatgcaatg taagtgcttc
     9481 attttcaact atttctccga atttctcaag gtcaccatat ttcaaacaat cagtaattgt
     9541 agttaaatgt gttttcgcct gtgagtatct gctatcggca aaaggattgc cttccatcag
     9601 cgcatggcca gcgcggctgg acactgattt ttcatctgac gacactatta aaatcgcatc
     9661 tttgaaggtt ttgaatgttg gatgtacttg ctcataaaat ggcactgcgt attcattact
     9721 cgagtcttgg atggcatcgt gctgtcccca aacggccaaa tatggataca cggaacggca
     9781 tgcactgcct gagcctaaac gagcaatcat actagctttt tctaagaaag accttgctag
     9841 catatcgcca ttcagctgct cttcaattgt tgttagacac aatgccaatg cagccattga
     9901 cgaagcggag ctggcaattc cagtagagtg aggaaaggaa ttctgcgaac taatttctaa
     9961 tgtcaaatat ggaagaaatg gaaaatgcgg aacgatgctt tctaaaaatt tatgaatctt
    10021 ttctgcaaat tttggattta cttttccttc aaacataaat tgtatgtcga tgtttttatc
    10081 gtctttagtt ttactataat acttcactac tgtttctgta aaagcattat ttagtgtaaa
    10141 actgatagag gcattgcgtg gtaattgttg gccatatttt ccccaatatt ttactaaagc
    10201 aatattagat ggcgtgcgcc aagctgtttc tccttgtttg ttttgaaatg gattcaagat
    10261 agatatttga gttgcaagat aaaaaaaagg tgagacaaat atcatctcac ctatatataa
    10321 tattactata cgctttacaa gccgtttata gaaataccaa atacaattgg attcattttt
    10381 ggtcctttag tagtaaatgc gctaccgata gaatagtagg caaataagct cagcggtcca
    10441 taaccagctc gtaaagttgg accatagcga aacttgctca tgtttgctac tttcatgtct
    10501 ttaatcgtca ctgtttctcc attgttaggc acatttggat cttcgccttt atatttccat
    10561 ttgctgccta ctaagaagcc gagtttaaaa ccaactgcaa ttttccagct catgccggtt
    10621 tttgcatgtg gttttgagcg atatctaaat tcaataggaa cgtcgatata cgtcatgttt
    10681 aattttgact ttttagaaga aatagaatcg ccaaatgctt ggaaacgtgt taaagtatcg
    10741 tagtgccacg ttaacttgta gtttttgaaa taataatttt cagaacccac accaatacca
    10801 ggtgcaatac taaaatgacg tggttttgtt tttttgccta ataaaatgtc gtacataaaa
    10861 tagacattga taccacgatt aaacgcgcct aatttaaaat cattaggcac cgttacgcct
    10921 ccgtctttct ttacgattgc attcatcatt gccaattcaa tgaccaatct atcgtttgcg
    10981 ccagtaattc tttttttaac tttttctgaa atgcctttcg ggccagcaag tgttgacttt
    11041 acgcttggtg gaattgaatc ttgtgcaata aggaatgtgc ttgcacataa catcaacaca
    11101 acgcttaaaa atatcttatg cataaatcga tttactctta aaaataagat gcaaaaataa
    11161 gattattaat tgattgtacg tgtgaattaa cctttattaa ataaaaataa ttcgataaac
    11221 ggtattatta aatagcataa gttttagata aaaccattaa tataccactg gcaagtcata
    11281 gtatctatta aatatattgg ttgcaaaaga caccataaac gagttggttt ttaaactgct
    11341 ggtacttgct aaattggtcg tgtattttct aaattgcaat tttgcttcga tgaccatatt
    11401 tattttcgga ttgatgatat agccaaatcg caaatcgtgg tccactactt tataaggaat
    11461 gccgttttgt attttaatgc cacttatatt gctggcttgt gaggcaatat ccaaaatatt
    11521 gtttccataa ttttgactga cagccgagtc tgcagcggtt ttaataaacg acattttgta
    11581 agatgtatag aatcgtttgt attgataatt tgccattagc aataattcat tgaaattggc
    11641 gcccatagga tgtgccagcg attggttata atgcgagtag ccgataccgc tgtcttgccc
    11701 ttggtaagta aatggtcgca cgctgttgta ttccatttgg aaattgagat ttttcacacc
    11761 accaacatca aaatatttaa caccagcttg ccagcccatt ctgcggtcaa ccgtattttt
    11821 tttaccatat ttattaatca taaattgacc atacaacaca acatatttcg ggcaagttac
    11881 ttttacattt aagccatata ctttagttgt atttggctct aatttcttct gaaaaccacg
    11941 aacgccgaga attggattga ggaaattata attaaaataa ttattgccat ttacagtagt
    12001 tctgcgccag gtgatacctt caaaaattcc gatttgcaag aactttgctg catcgatact
    12061 tagataattg aagttggcta atttgcgagg aaagccttgt ccaaaatcgt tgaaatttct
    12121 aatgttatcg atgagttctg cataaattgt cgtgtacttg atgcgccaaa cattcgccgt
    12181 taattgtaaa tatggatagg tataagcatt atcagacaac agtaaagagc gatagccatc
    12241 gccaataaaa tgcttgccat tgccgacttg tatattaaat tgcttaactg gtgaatagga
    12301 aatataggca taaatactgg agaaatcgag cccttgcttg ccacgatttc tgacatttcc
    12361 ttcgccaggc accacgcctg tttgcgcgtt atatatatca atatatgtcg gaaacttgcc
    12421 tactgtattt aagaatgctg tgtaaagagt cactttttta ccaatgtcgg cgcgtaattc
    12481 taagcctttt aagttgtagg ttaggaattt tttcgagctt acatcatatc caaatccaat
    12541 atttaatata ggattgagcg acacatatac ttttcgtttt ttatacgctt tatcataaat
    12601 attgtcttca taaaaaacgg aatctttcgc caatattttt ccataatgtt tgacatagcc
    12661 tttctcatca aattctaata agttttcgaa accaaccaca tttactagct tgctaaacca
    12721 attggtgtgt ggcacacgct cgtaatgctg tacagtgtct ggaaaaacaa actgatagac
    12781 ttctgctgcc gtatatggtt ttacggcaga gtggaaagag gccttgtccc aagcgtttaa
    12841 tgacttctca taattgagta tcatgtttct gttgagtggc agattttctt gttgtgcagt
    12901 agcggaaaga gcgtacgata acagcagaat taggaatacg taatttttag aatgcatttt
    12961 cttcacctcg aatcatattg attatagtca tgataataat tttgacatca agtccaaaac
    13021 tccaattttc gatataccat acatcggcac gcacgcggcc ttccatatct gtcacatctt
    13081 ttgtttcgcc tcgatatcca tttacttgtg cccagcctgt tatgccaggt ttcaccaaat
    13141 gtctgaccat aaatttatca attattttgg aatattcttc agtatgtttt agcatgtgcg
    13201 gtcttggacc aataactgac atttgaccaa agaatacatt tataaattgt ggcatctcat
    13261 ctaagttggt tctgcgcaat aattttccta ttggcgtgat gcgttggtca tcgcgcgtgg
    13321 cttgtttgta atgcgcgtca ctattgatgt acatcgttct aaatttaatt acttggaacg
    13381 atttattttt gatgcctgag cgttgttgca cgaagaatac gggtcctttt gaagtaattt
    13441 ttattaaaaa tgcaaccaat ggtataaacc accacaagat gaaaagaaat accaaaatag
    13501 aaaatccaag gtcaaacaat ctttttataa aacggttgac aatattctct aaaggctcgc
    13561 ttcgcaaggt cattactgga atttgtccat aataatcgat ttctactttg tttaaatgat
    13621 gtgttaaaaa gcgtgataaa tctggaacta ttttgacacg aataagattt tcatctccaa
    13681 attgaattaa tgatttgatt ttttcttctt ctttataggg caatgcgcag aacaattcgt
    13741 ctatctgatg tgtgatggca tattgttttg catcttcaat attacctaaa atattttttt
    13801 gaatttctgg tttaaaatgc gttggatggt cgtcataaaa gcctaaaaac tgatagccat
    13861 attctatttt atgctccaac atggctttaa actcttgtgc tgtatcgcct gcaccaatta
    13921 tcatcacttt gcgattatta aaaccacgcg ctctgtaaaa tcgtacaatt ttaataaagc
    13981 caaaacgcca catcatcgtg aagaaaaaag ccactgtata ggtaatcaaa agtaactcac
    14041 gtgaaagttt gaatgtctga ttaaaatctt tgaaaacgac aacataggaa aatgcaaaaa
    14101 gcacttcaaa aaagaacaat cgcaacaagg ccgataataa tgtgaaaaat cgtgtgttgc
    14161 gatacatatc aaatgtgcca gatagttgcg ccgcaaacaa gtaaattaca ttcaaatata
    14221 tccacaattg gagatactta gtgtcgaata tcattcggta attgtcgctt aaaaaatggc
    14281 caataatata ggcagtatta agaataaaca aatcgcctaa tgcatgcatt aaaacgtata
    14341 aatttgttat cctttttttc attttcttag tgttctttag caataaaaat tacatataca
    14401 gcaatcagac cgcttataat tgtaatggat aataaaaacc tacggcttgt tggcgcaagc
    14461 aatctgcatt atctgtatag atttataatc taccaaaaac gttgcagcta cttagttggg
    14521 catcttgcaa acaaaaacct ttctccttta acaaatttac tacagaaaag ctaaaccacc
    14581 actatttata tcatttatat tttaattttt gcattttcat agttcttgag taacaattta
    14641 ttgtgaacta gcttcaaaaa tgaaattgtg catatttttt tctgtataat ttatggaaaa
    14701 tctttcattt cggtctctta atagtataaa tttgttcaac gataaaatag atgtaatatg
    14761 agtaaagaag acaaagcaaa caaagaagca ggcagtgaaa aggcgaaagc cttattatta
    14821 accttagaga aactggacaa acaatacggc aaaggcaccg taatgaaatt aggcgacaat
    14881 agcattgtag agtcagaagt tatatctact ggctcattag gtttggactt agccttaggt
    14941 attggtggaa ttccaaaagg aagaattatc gaaatatacg gacctgagtc ttctggtaaa
    15001 acaacactgg ccattcatgc aattgcagag gcacaaaaga aaggtggtat ctgcgctatc
    15061 atagatgcag aacatgcatt tgacaaaaaa tatgcggaag cattaggcgt tgatacagac
    15121 aatttatata tcacacaacc tgatgatggc gaacaagcat tagacattgc cgagcaatta
    15181 attcgttctg ccgctatcga cttagttgtg gtggactcag tggcagcatt ggtgcctcgc
    15241 agtgaaattg aaggtgaaat gggcgatagc aaaatgggct tgcatgctcg actgatgagc
    15301 caagccatgc gtaaactaac tggtgccatc aataaaactt cttgcagttg tattttcatt
    15361 aatcaattgc gtgaaaaaat tggtgtgatg ttcggaaacc ctgaaacaac aacaggtggt
    15421 aatgcgctta aattctatgc ttctatacgt ttagatatcc gtcgtgctgg tcaaattaaa
    15481 gataaagaag gtaatgtcat cggcaatcaa accaaagtga aagtggtgaa aaacaaatta
    15541 gcaccgccat ttaaagttgc tgaattcgac atcatgtttg gcagaggtgt ttctaaagaa
    15601 ggagaaattg tagatatggg cgccgagttg ggcatcttac aaaaaagtgg tgcatggtat
    15661 agctacgaaa gccaaaaaat tggtcaaggt cgcgaccaag tgaaacaatt aatgttagac
    15721 aatccaggat tggcggatga aattgaagga aaaatcagag aaaaattaaa aactgtataa
    15781 gcaaatagtt tttaataaac aaaataggaa cgccatttca tcactgaaat ggcgttccta
    15841 tttatgtata cacaaaaaaa tagcgcttat acagaattct gaaaaacaac ttgtgactat
    15901 tctttatgca ccttacaaag aaaagaggct atctcaaaaa ttgaaatagc ctcttttaaa
    15961 tattattttt gtttaatctt aaaatttata gaatttcacc cagtcaattt ccatttccac
    16021 tggtaattgg ttgccatcaa ctgtaccgac ccagcttcct ccaagctgca tatctaaaag
    16081 caaataatga tcgccatcag catatgggaa ttgatctggt tgtgtagttt gaatttttgg
    16141 ataggtaaat gtttttgtgc catttacata aaacactact ttgtcagcat atttttctac
    16201 agcatacgtg ttatacgcat tcggattaat ggccgctgtg ccataatttt ttggattgtc
    16261 tttgattcct aaatttatag tgtaagtaga atgtactgtt tgatagacaa aattatcact
    16321 gtttaaacgc tccatgatgt ctatttcacc accttgcgga taaggacgac cttgtgcaag
    16381 caaccagaaa gctggccatg caccttttgc tgcattcagc ttagcgcgaa tttcaagtct
    16441 tccaagacca aatgctactt tatctttagt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnn gctctaatga caaatacaat gttcgttatc attgacttat ctaacacaaa
    16621 aatactgctt atattttatc aaattgagta aagacaattg acaattgata agaaaacaag
    16681 atgtcaaaca aaaaataatt cagccaattt aaaaacgtgt tattggtatt ttaattcttt
    16741 tttcaattat aaatttcgat attcgtagaa tagacacaca tggaattttt acaaactatc
    16801 atttctgatt ttaaggcaca ctggtacatc tatttatcaa tgcctattat tgctgcattg
    16861 attggctatg gaactaaaat tgttgccata aaaatgatgt tcgagccatt agagtttttt
    16921 ggcattaaac cgtttcttgg ttggcaaggt attattcctc gcaaagcagc cgtcatgagc
    16981 actatagcat gtgatacttt aacggctcgt ctcatcaaac ctgaagaaat ttttgccaga
    17041 ctcgatccga accgaattgc acaagaatta gaaaaaccta tgttgccgat agtagaaaaa
    17101 atcacacacg acgtgatgag ccactatcaa ccaggtttat gggaaagcat tcctgaaaat
    17161 gtcaaaaaaa tgctcatcaa acaaatccag cagcaatcac cagatatggt gcgccagctg
    17221 atgcagcaaa caaaagacaa tctcaacgaa gtgtttgatt taaaagatat ggtggtcacc
    17281 aatctaacta aagacaaaaa attactcaac aaaatatttt tagaaagtgg cgaaaaggaa
    17341 ttccaattta tcagaaattc aggaatatat tttggttttc taataggact agtgcagatg
    17401 gtcgtttgga ttttaacaca caatgtttgg gtaatgccgt tatttggttt atttacaggt
    17461 tggtttactg attggctggc gctaaaaatg atatttgcac caaaagaacc taaaaaatat
    17521 tttggtttgt ttacttggca tggtttattc tttaaaagac aacaagaggt ggcatcaaag
    17581 tatggtgcat taatagcgaa agaagtgcta acgcctgcca atatgattga gtctattctc
    17641 actggaaaaa tgtctgataa tctatttaat atgattcaaa aaaacgtaca gcagatggtt
    17701 gatgaacaag caggcatttt aaaaccagtt gtcgtatttg ccgtaggttc agaaaaatac
    17761 aaagccatga aaattgcgat tactggaaac atggtgcatc aattaccagt tgctttgaaa
    17821 catatcgaaa aatatgcgga agatgcaatg gacatacaaa acacattagt cagcaaaatg
    17881 caagaattga cacctgaaga atttgaagga gtattgcgac ctgcgttcaa acaagatgaa
    17941 tggattttaa tcactgttgg agctgtttta ggatttcttg tcggcgaatt acaggtactt
    18001 attatggagc actttgtttt gcgctgagaa atttagttgc aattacaact ttcatttaaa
    18061 caattcttaa ctttctactt atgctatgca tactttgttt ttgtaatttt atttaattct
    18121 tttaatgacg ctttgtcatc gtgaacgttt tgtagccgct cttgtaaaaa acattatttt
    18181 atcagaaaag atacatcgtg ccagcgcatg acaagaaaac ataacataat gagcaacaaa
    18241 gtttttatct tcgacacaac actacgcgac ggcgaacaag ttccaggctg tcaattgaat
    18301 actattgaaa aaatcgaagt aggcttagca ttagaagcat taggcgttga tgtgttagaa
    18361 gctggttttc caatttcatc acctggcgat ttcaaatcgg tagtcgaaat taccaaaaac
    18421 attttcaatc caactatttg tgcgctcaca cgagccgtag ctgcagacat tgattgcgct
    18481 gcggaagcat taaaacacgc taagcgtggt cgtatccata caggcatagg ctcctcagat
    18541 attcatatca aaaataaatt aaaaacaaca cgtgaagcca ttttagaaaa agctgcttgg
    18601 gccgccaaat atgcacgtaa ctatgtagat gatgttgagt ttttctgtga agatgctgga
    18661 cgtgccgact tggagttttt agcacgcatg gtagaaactg taattgctgc aggtgctaca
    18721 gtagtcaata taccagacac tacaggctat tgcttgcctg aacaatacgg cgcaaaaatt
    18781 gcgtatttga tgaataatgt gccaaatatc gacaaagcaa ttatttctgc acattgccac
    18841 aacgacttag gcatggcaac agccaactct atggctgctt tgcaaaatgg cgcacgtcaa
    18901 gtagaagtaa ccatgaatgg cattggcgaa cgcgccggca atacttcctt agaagaaatt
    18961 gccatgattt taaaatcgca tgaaaataat ttgaatttac aatgcgatat caatatcaaa
    19021 aaaatatatc ctacatcacg cttgattaca agattgatgc gtatgcctgt acaacctaac
    19081 aaagcaattg ttggccgtaa tgcatttgct cattcgtctg gtattcacca agatggcgtt
    19141 ttaaaaaata gagaaaacta cgaaatcatc gacccacatg acgtaggcat tccagaatct
    19201 ttaattgttt taacggcaag aagcggacgc gcagcgctga agcaccgttt ggaaaatatt
    19261 ggattcacct tagaccaagc tcagctagac gaaacatata agaaattctt agacatggca
    19321 gaccaacgca aagaaatctt cgacgaagat ttgttggaaa tgatgggcca aagccgagaa
    19381 ggaaaaattt atgaaataga ctatttagaa gtgcattgcg gtaaacgtca tcaagctaca
    19441 gcgacagttg ctttagtaaa aaatggaaag cgcacagtag aaatcgcaga tggctgtggc
    19501 ccggttgatg ctacgctcaa ggctattgac aaaattgtac aacagcccgt acgtctcgaa
    19561 gaatacttag tgcaagcagt aaccggcggc agcgatgaca ctggcaaagt acacattcaa
    19621 atttcgaaaa atggtgtgtt ttactatgg
//
LOCUS       DAOQQV010000039        19431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_852_length_19431_cov_8.356678, whole genome shotgun sequence.
ACCESSION   DAOQQV010000039 DAOQQV010000000
VERSION     DAOQQV010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19431
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_852_length_19431_cov_8.356678"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLL59_05780"
     CDS             <1..327
                     /locus_tag="PLL59_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HMY23350.1"
                     /translation="KIIKQIAAYKNIKKIYITENGVAFKDSLVNGNINDTNRIQFLKD
                     YLKQILRAKKEGVDIGGYFVWSFMDNFEWAEGYHPRFGLVHVDFKTQQRIIKDSGKWF
                     SEFLQK"
     gene            344..1780
                     /locus_tag="PLL59_05785"
     CDS             344..1780
                     /locus_tag="PLL59_05785"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HMY23351.1"
                     /translation="MKKTIPFWIILFSILSANAQQHLFSGKPVIHTPTEIGNYPNTPF
                     LYKIPATGERPLQIQVSGLPQGLVFDDKTNIITGQINTASKFNINITATNKEGSTTKS
                     STLVFGDKLCLTPPLGWNSWNVYAHEVTEDIILKVADAMQKNGMQDVGYEYINLDDYW
                     HADQREANGNPKADSIKFPHGIKWLADQLHSKGFKLGIYSCAGDMTCGRRFGGYNYEE
                     IDAKQYAAWGIDLLKYDYCFVPKGKEIAMTRFIKMGNALKNSGRSIVYSLCEWGKNEP
                     WLWADSANAHYYRTTDDIFERWNGLSFGYEGVFSIVNQNEKLVQYAKPGHWNDPDMLL
                     VANNGNGLATGKQKRFKGLSDAQYGSHFAMWCFMNSPLLSSADPSKLNDKNLNLLLDS
                     TLLAIQQDVSHQQASLLSKKNGIYVFKKQLSNNKTAYLFVNKSKRNKNVKLADFINGT
                     AISNQPKFISNNKESLSIAAHDYSIILQ"
     gene            1939..3006
                     /gene="serC"
                     /locus_tag="PLL59_05790"
     CDS             1939..3006
                     /gene="serC"
                     /locus_tag="PLL59_05790"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HMY23352.1"
                     /translation="MKKHNFGAGPSILPQEVFEQSAQACLDYNGIGLSLLEISHRSKE
                     FTAILEESISLIKSLLNVPDDYSILFLQGGANMQFNMIPYNILNEEETALFIETDIWS
                     TRAEEETRIYGKTKIIASSKADQFKYIPKDFTVDKDAAYLHITSNNTIYGTEFFEDRD
                     YGVPLVCDMSSDIFSRPIDVSKYHLIYAGAQKNLGCSGITLVIIKNEWLGRAKRAKSK
                     MWDYSVYAKNNSLYNTPPVFTIYTCLLTLRWLNKLGGLQVMEQRNKAKAELLYAEIDR
                     NPLFAGHAAVEDRSRMNINFITTHAEDEDKFLEFVKPYQIHGIKGYRTVGGFRASCYN
                     ALPIESVQVLVQAMQDFEKAR"
     gene            complement(3020..5221)
                     /locus_tag="PLL59_05795"
     CDS             complement(3020..5221)
                     /locus_tag="PLL59_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23353.1"
                     /translation="MHNFPFSLRIVFSFLLFFSIQLQTVAINYPSIQQPGCATFETQN
                     DNWTLKNNLLTATFIKQGNKLIFGGVAEMNLIKGTELFEIHLGNGTKIMSSEMICKSL
                     EAENLEANSQALKRSLQVAGKAVKAAFEYKSLTVLWRAILRDSSHYLRFELSVYSKEN
                     IQLQSITPLQFTVKNIAGNTTPTIVGNTRGSVIASNCLFAAVETPMATLSQNNISLAS
                     FVEAKSPNITPNTKLYDVNKSETNTNAENYTSISGKWNRNTILAIGDTFHVSSVVGMI
                     ADGQRRRSFLAYLERERAVPWRSFIIYNSWYELNINRNDDKDPRKRMNEAQCLSVLDS
                     WKSNLFTKHKTNIDAFVWDDGWDNFNSLWDFHVGFPNGFSSVNAKAKEQNCGIGTWLS
                     PVGGYGASKKNRLAFWNGTHGTRLKNFQLSNKEYFDAFVSSCSQMVSNYNMRYFKFDG
                     ISDFFSAVGPKNDEDAEGIINVINVLRKQQPDLFINSTVGTWASPFWLRYSDAVWWQE
                     GDFGTIGNQGNKREKWITYRDRLVYQNFVQNAPFSPINSIMTHGLIVTKYGPPSEMPH
                     DNSKATFDGIVKEMRCAFACGTNMVELYLDSDLMSNIGEDGALWAALADCIAWHRSNA
                     DVLADSHWVGGNPWDGKHANVYGWAAWNEQKSTLTLRNPSGMNKLFSTTLRQALEIPN
                     HINGKIVLKSAFNNQTNYADIIGKEIDIDALINFNMPPFDVIVLNGVSLAN"
     gene            complement(5314..5925)
                     /locus_tag="PLL59_05800"
     CDS             complement(5314..5925)
                     /locus_tag="PLL59_05800"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HMY23354.1"
                     /translation="MAFELPNLPYAHDALEPHFDTLTMQIHHGKHHNAYVSNLNAAVA
                     GTENEGKSLEELMAKISTLPAAVRNNGGGHFNHSLFWTILSPNGGGEPTGAIADAIQA
                     TFGSYDKFKEEFTKAATTRFGSGWAWLCVKADKSIAVCSSPNQDNPLMDIAECPGTPI
                     LGLDVWEHAYYLHYQNRRPDYIAAFYNLINWDEVNARYAAATK"
     gene            6673..7260
                     /locus_tag="PLL59_05805"
     CDS             6673..7260
                     /locus_tag="PLL59_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23355.1"
                     /translation="MSFLWLEDGRKFKAYNIYFNDTKQISSIKETDTLGNNEAHTTHI
                     FRLNNNIDSIFDIGYAGSFIESGTYISNISYNNNNCKSFLLQYSTYYPTNTNVDDTMQ
                     FYFSQQPNTNMLPYQLPDANAGGSIFRLLSYYLSINGYYAFNPNINLIDSIKIADVTI
                     RYNYEFINNFVSKARYITERDNGSSYTNNNIFEYY"
     gene            complement(7272..8510)
                     /gene="clpX"
                     /locus_tag="PLL59_05810"
     CDS             complement(7272..8510)
                     /gene="clpX"
                     /locus_tag="PLL59_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HMY23356.1"
                     /translation="MAKKVISKCDFCGRAKEDVQMLISGQDAKICDACVEQAMQLLNE
                     EGFVQHEKPNYKGEPNKNLKPLAIKAFLDDYVIGQDEAKKVIAVAVYNHYKRLYQKTT
                     EDDVEIEKSNIIMVGQTGTGKTLIVKSIAKLLNVPFTIVDATVFTEAGYVGEDVESIL
                     TRLLQVCDYDINAAEKGIVFIDEIDKISRKGDNPSLTRDIKEGTQQSLLKLIEGTEVL
                     VPPQGGRKHPEQKLLKVDTKNILFLCGGSFDGIDKIISKRLNTSVIGFKNKKGSEEVD
                     KENLLNYITHQDVRAFGIIPELLGRLPVLVHLDPLDKNALTNILTQPKNALLKQYKKL
                     FAIEGITLHFEAAAIDFIVDKALEYKLGARGLRSICEAIITDAMFELPSKTDVKEYTI
                     TEAYAKQKLSKSKLAKLKVA"
     gene            complement(8681..9352)
                     /gene="clpP"
                     /locus_tag="PLL59_05815"
     CDS             complement(8681..9352)
                     /gene="clpP"
                     /locus_tag="PLL59_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HMY23357.1"
                     /translation="MDYGKEFRKYATKHHGLSSIYVDNYLQTNITNLTPNIIEERPMN
                     VAALDVFSRLMMDRIIFMGTGIDDYVANVIMAQLLFLDSADRTRDIQIYVNSPGGSVY
                     AGLGIYDTMQYVEPNVATCCVGIAASMAAVLLCAGEKGKRSALKHSRVMIHQPMGGAQ
                     GQVSEIEITYKEITKLKKELYDIIANHSGQPFEKVEKDSDRDYWMTSEEAKGYGMIDE
                     VLIRK"
     gene            complement(9440..10531)
                     /locus_tag="PLL59_05820"
     CDS             complement(9440..10531)
                     /locus_tag="PLL59_05820"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HMY23358.1"
                     /translation="MNISQKNTDSLNAIISIELKKEDYLPNVEKALANAKSNAAIKGF
                     RKGHVPTSLIKKMYGNSILLDELNKAVSENLNKHIEDSKLNMLGRPLPIPSETLEIDI
                     NNPVDYTFEYEIGLAPEVKVDALSKKTSIVKDVISIDEKTLTDEVEKLRERHGNLINP
                     DRAEGEEAKYEKVELNQELFDKVYGPNIVTSVDEFKEKIRKELQDFSDQSVVNKFKDN
                     IYTTLMEKTEVKLPDAFLKKFIKSSNEKPIDDEQIEQEYPQFEKGLKWNLITNAIAKE
                     NDIKIEFEDVKNFSKEQIRAQFMMYGGGITDDMIESFNNSMMAKEEHVKKSYDGALEQ
                     KLFTFIESQVTVEEKTTTFDEFFNKKDEK"
     gene            complement(10618..10697)
                     /locus_tag="PLL59_05825"
     tRNA            complement(10618..10697)
                     /locus_tag="PLL59_05825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10661..10663),aa:Leu,seq:tag)
     gene            10797..11399
                     /locus_tag="PLL59_05830"
     CDS             10797..11399
                     /locus_tag="PLL59_05830"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMY23359.1"
                     /translation="MKNISLILNVILFILVGVLFYLQFTEKKGSSAVPQVIKTDGKSV
                     TVPQIAYIDIDSFQANYDYFKTGKTKLEAKQKSMEAELERSMSAFQAEYNSLVQKAQT
                     MTEEEGMAAQQKLAEKQQKIEERKQSMEMQFMKETSDFNIELQERIIAYLKKYNANGA
                     YTYILPYSRETINLLYVNDAYNITTEVLKGMNEEYKTNKK"
     gene            11488..13716
                     /locus_tag="PLL59_05835"
     CDS             11488..13716
                     /locus_tag="PLL59_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HMY23360.1"
                     /translation="MEVNVEAEKQAIIKDYRQLLRLARPRMKKGDDKRIRHAFELAVD
                     AHKHMRRKSGEPYIHHPIAVAKIVAGDMGLGTTSVICALLHDTVEDTDLTIDDVKKEF
                     GNTAAKIIDGLTKISKISHQTESIQAENYRKILLTLSDDIRVILIKIADRLHNMRTLD
                     GQSRKNQLKIASETIYLYAPLAHRLGLYSIKGELEDLAMKYTESEKYKEIAKALESTK
                     KDRTKYINDFVRPLQEEMDKSGLKANVFGRPKSISSIWNKMRKKEVEFDDVYDKFAIR
                     VILDSAQENEKADCWRAYSIVTDKYSPQPNRMRDWISHPKGNGYESLHTTVMGSGGQW
                     VEVQIRSQRMDEVAEKGLAAHYKYKENKFKAANSAEEAIESWLMQVREFLKNPDVNPI
                     EFINDFKLQLYQKEIYIFTPKGELKILPATATALDFAYTVHTNVGDNCIGAKVNNKLV
                     PISHELKNGDQVEIITSRKQKPSEDWLNIVTTSRAKSKIKNSLREEKRRIADDGKEIL
                     ERKLSQIKANFSNDNINLLTNLYKMPSSMDLLYGIAKGTINLTELKQLRNVGGALEII
                     KPEKIGKPKDEEVFYKTEDNNGDIPKNAELVIFDESSKFPYEFAKCCNAIPGDDVIGF
                     ISINGVIKIHRTGCPNVINLASKYGYRIIKTRWTAKTTPAFLTTVKLTGLDDIGVISK
                     LTTIISGEMKLNMQSISLETNEGIFEGIIKVFVKDNEQVNKLISKLKSIHGIDSVIRE
                     EI"
     gene            complement(13713..15608)
                     /locus_tag="PLL59_05840"
     CDS             complement(13713..15608)
                     /locus_tag="PLL59_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23361.1"
                     /translation="MISCTHKQSFNSFILTTQNHKSNNSNFKLVDIGTIAYYNAFTKA
                     IQLSPKHNFGFTIEKNVKPKTYYHLQLKVHSKNYSVNLATESNFYGWIGSHNPTFQDK
                     SKEWYYINLFIKTPDTIKQGIMKIYAYNWTNDTAYVDSLKVTEYSEFPFQDSVPDFIF
                     KPLTYNLLNELAFYGKNVSVENFLDLLDEDILFSVFDKQFIPKKEYIKELNRRTKKCK
                     LNDIFDMIKQDANILKNPSVNTKPRETFETKLSGYTDKIIYTKGEQVTIKIQNAHQLN
                     SVQLLQPIENYKFRKLMDIAVLNKENIIIETKDLSPNTYCIQLKDKQSVFNIPFIISS
                     NQQSDIVLLAPVTTWHAYNYYGGKSFYVNTKDDSCVYTISTQRPLVSCTFDSILVGHD
                     LFIFDNIYKFFFQNYQCNVYPDYYLDAHPELFKNAKTIIFAQHCEYFSTKMFNALAQF
                     SKSKNIISLGGNQAYWKIRFSDNYQKIECRKDGTFFENTLIPAGLWRTSFSNEAKYLG
                     NAYTDAGYETYCSYKVINAQHWLYKNCTIKNGDEFGKYGIDNRGLSGDEMDKVNDNTP
                     KNSVLLAKGTNPDNGGGDILIIENKNNAILSFGSIACGSGLNKDKVFTQMTKNFLERY
                     HKKQIQH"
     gene            complement(15701..16792)
                     /locus_tag="PLL59_05845"
     CDS             complement(15701..16792)
                     /locus_tag="PLL59_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMY23362.1"
                     /translation="MNNLSVMNLPTSSPHIADSKIQFVLDTIYFWNDTFGWDGTIDNQ
                     VPAGSEKGDSLYTTYVVNKHGVKNKYTTLHVFISGLGGNVNGHGGGQAKWFGAKEWVL
                     VNSIYQAYLNNTMWNASGLLRHELGHALGLYHSWSGDNCNDTPNNPNCWDNTQPGCNP
                     NSNNTMDYNVSQDALTRCQINTVHYFLLGNQGNISDCIISGVNVTTPIVSGPTSICNP
                     NGSNYTINNLQLGVNDNATAYPSTLFQNADICGGPVINFKPASTASSGGAILTFTTGF
                     GVYGNTFITKRVWVGTRPSVLNVCSFTNNPQIYGATTINSPGTGCTSTSVPLNQDVTF
                     IAETTNLNPGFEVPLGATFEVIPEIISCQ"
     gene            complement(17115..18011)
                     /locus_tag="PLL59_05850"
     CDS             complement(17115..18011)
                     /locus_tag="PLL59_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23363.1"
                     /translation="MKKNLLLLSFFIITFTQLKATDWFPIGAKWSWDYTLCCPDGPYT
                     EKWVVEKDTIIGIDTFQILNQVNYHTNSVRRLIIKEENNKVYRWLNNELLPICDFTKS
                     EHDTCLFVAPNSSNILDSFFYIIDSIRPLQQNSTIKVQYGILKPKTTTVFPSYHLEIK
                     EKACFWGFTNSSYFLSTEPPILNLRCYQDTELSINFSSLACDSILALIKEKYNNQLQI
                     EIYPNPISDKILIEYYGTKNYQKAEIIILDINGKIIKPINLIDNMLKFKEISLSDVLN
                     GIYFLKISIDDEFTIKKIIVNH"
     gene            complement(18019..19077)
                     /locus_tag="PLL59_05855"
     CDS             complement(18019..19077)
                     /locus_tag="PLL59_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HMY23364.1"
                     /translation="MFSSTTSNAQIKVISNGNVGLGVSSPQGKLHINDVLLLGPKTGV
                     SYSEGIKFTRSQNYPTSPAEWAFDTDGSGFNIWKPYPSTNYGNFKLFIKDANGYVGIN
                     TGNPSYRLDVNGSLRCYGFINSSDERLKSNVIEIKNALDLILKLTPKTYDLTIPVNDQ
                     SIADELKKTNNSKDKIKPEILYNQAGLVAQELKAVLPNLISEDNKGYLGINYIGLIPY
                     LIQSIKEQNKKIQDLQRQLQQCCTNKLYDSEKSETERNKIIENTPNTYAFLEQNNPNP
                     FHQQTQISYTIPNNCTNSSLHIYDLNGVELSSFSINQSGKGSIFIDGNSLKAGMYLYT
                     LICDGKEIATKKMILTSK"
BASE COUNT         6422 a         3264 c         3108 g         6637 t
ORIGIN      
        1 aaaattatca aacagattgc tgcctacaaa aacatcaaga aaatttacat taccgaaaat
       61 ggcgtagcgt ttaaagactc tttagtaaat ggcaacatca atgatactaa tcgtattcag
      121 tttttaaaag attatttgaa acaaatactt cgtgcgaaaa aagaaggcgt tgatattggt
      181 ggttactttg tgtggagctt tatggacaac ttcgaatggg cggaaggcta tcatccaaga
      241 tttggattag tgcacgtaga tttcaagaca cagcaacgta ttatcaaaga ttccggaaaa
      301 tggtttagcg aattcctaca aaaataaaat attattacac atcatgaaaa aaacaattcc
      361 attttggatt atcctattta gtatattaag tgcaaatgca caacagcatt tatttagtgg
      421 caaacctgtt attcatacac ctacggaaat tggaaattat ccaaatacac cgtttcttta
      481 taaaatacca gctacaggcg aaaggccatt acaaatacaa gtgagcggtt tgccacaagg
      541 tttagtattt gatgacaaaa caaatataat tacaggacag attaatacag cttcaaagtt
      601 taatattaac atcactgcaa caaacaagga aggcagcacc acaaaaagtt ccactttagt
      661 ttttggcgat aaattatgtt taacaccacc attaggctgg aacagttgga acgtatatgc
      721 acatgaagtt accgaagata ttattctaaa agtcgctgat gccatgcaaa aaaatggcat
      781 gcaagatgtt ggttatgaat atatcaatct tgatgattat tggcatgctg accaacgtga
      841 agcaaacggc aatccaaaag ctgattctat taagtttcca catggcatca aatggttagc
      901 agatcagttg catagcaaag gttttaaatt gggaatttat tcttgtgctg gcgacatgac
      961 ctgtggccga agatttggcg gttacaatta tgaagaaatc gacgcaaagc agtatgcagc
     1021 ttggggaatt gatttattaa aatatgatta ttgtttcgtt cctaaaggca aagaaatcgc
     1081 aatgacacga tttataaaaa tgggaaatgc cttgaaaaat tctggtcgtt ctattgtata
     1141 tagcttgtgc gaatggggaa aaaatgaacc ttggctttgg gctgatagtg ccaatgcaca
     1201 ttattacaga acgacagacg atatctttga aagatggaat ggtttgagct ttggctacga
     1261 aggtgtattt agtattgtca atcaaaatga aaaacttgta caatatgcaa aaccaggtca
     1321 ttggaacgat cctgatatgt tgttagtcgc taataatggc aatggtttag ctacaggtaa
     1381 gcaaaaacgt tttaagggat taagtgatgc acagtatggt tcacattttg caatgtggtg
     1441 ttttatgaat tcgccattac tttcctcggc tgacccttct aaactaaatg acaagaatct
     1501 aaatttactg ttagacagta ctttattagc tatacaacaa gatgtgagcc atcaacaagc
     1561 atcattatta tctaagaaaa atggcattta tgttttcaaa aaacaattga gtaataacaa
     1621 aactgcttat ttatttgtca ataaatcaaa aagaaataaa aatgtcaaat tagctgattt
     1681 tattaatgga acagccattt caaaccagcc aaaattcatc agcaataaca aagaaagttt
     1741 gtcaattgca gcacatgatt attctatcat tttacaataa gaagtctgaa atataaatct
     1801 cactaaaatg accatggttt tttagccatg gtttttttta ttttcttcat taataattct
     1861 ataatctaag gttgatacat tatttagtaa tttcgttaca atactgaatt ctattaggtt
     1921 tcaagaaaat attaaagcat gaaaaaacac aatttcggag caggtccatc tattttaccg
     1981 caagaggttt ttgagcaaag cgcacaagct tgtttagatt acaatggcat cggactttct
     2041 ttattagaga tatcgcacag gagcaaagag tttacggcca ttctcgagga aagtatttcc
     2101 ttgataaaat cattactcaa tgtaccagat gattatagca ttttattctt acaaggtggt
     2161 gctaatatgc aattcaatat gattccttac aacatcttaa atgaagagga aacggcattg
     2221 tttatcgaaa cagatatttg gtcaactcgt gccgaagaag aaacacgaat atacggaaag
     2281 accaagatta tagcatcttc aaaagcagat caattcaaat atattccaaa agattttacc
     2341 gtagataaag atgcagccta tttgcatatc acgtcgaata acactatata tggaactgaa
     2401 ttttttgaag atagagatta tggcgttccg ctagtttgcg acatgagttc tgatattttt
     2461 tcgcgtccaa tagatgtttc caaatatcac ttaatttatg caggtgcgca aaaaaacttg
     2521 ggatgttcag gaattacttt agtcattata aaaaatgaat ggttaggcag agccaaacgt
     2581 gcgaaatcga aaatgtggga ttatagtgtg tatgctaaaa acaattcatt atacaacaca
     2641 ccaccagtat ttacaatcta tacatgcttg ctcacattac gttggttaaa taagctaggt
     2701 ggcttgcagg ttatggagca acgcaataaa gcaaaagccg aattattgta tgctgaaata
     2761 gacaggaatc ctttgtttgc tggacacgca gcagtagaag accgaagccg aatgaatata
     2821 aattttataa caacgcatgc tgaagatgaa gacaaatttt tagaatttgt aaaaccttat
     2881 caaattcatg gcatcaaagg ctatcgtact gttggtggtt ttcgtgcatc ttgttataat
     2941 gcattgccca ttgagagcgt acaagtctta gtgcaagcca tgcaagattt tgaaaaagct
     3001 agataagata aaactttaat tagttagcaa gtgaaacgcc gtttaataca atgacatcaa
     3061 aaggtggcat attaaaattt atcagtgcat caatatcaat ttcctttccg ataatgtcag
     3121 cataatttgt ttgattatta aaagcagact tcaatacaat tttaccattt atatgattcg
     3181 gaatttcgag tgcctgccta agtgttgtcg agaagagctt attcattcca cttggattac
     3241 gcaatgttag cgtagatttc tgctcattcc atgctgccca accgtaaaca ttggcatgct
     3301 taccgtccca aggattacca ccaacccaat ggctatcagc caatacatca gcgttactac
     3361 gatgccacgc gatacaatca gctaatgctg cccaaagtgc tccatcttcg ccaatattac
     3421 tcatcaagtc actatctaaa tacagttcta ccatgtttgt accacatgca aaggcacaac
     3481 gcatttcttt cacaatacca tcaaacgtag ctttggaatt atcatgcggc atctctgaag
     3541 gtggaccata tttagtgacg attaatccat gtgtcataat tgaatttata ggactaaaag
     3601 gtgcattctg aacgaagttt tgatatacca aacggtctcg atacgttatc catttttcac
     3661 gtttgttacc ttgatttccg atggttccaa aatcaccttc ttgccaccaa acagcatcac
     3721 tataacgcaa ccaaaaaggt gatgcccaag tgccaacagt agaattgatg aataaatctg
     3781 gctgctgttt acgaagcaca tttattacgt tgataatacc ttctgcatct tcatcattct
     3841 ttggtccaac agcactaaaa aaatcactaa tcccatcaaa tttaaaataa cgcatattat
     3901 aattgctgac catttgcgag caactgctaa caaatgcatc gaagtattct ttattggaaa
     3961 gttggaaatt ttttagtcgc gttccgtgag tgccattcca aaatgccaat cgattttttt
     4021 tggaagcacc ataaccgccc actggactta accatgtgcc aattccacaa ttctgttctt
     4081 tagctttcgc atttacggaa gaaaatccat tgggaaaacc aacatgaaaa tcccataaac
     4141 tattgaaatt atcccaacca tcatcccata caaatgcatc aatatttgtt ttatgtttgg
     4201 taaataaatt actcttccac gaatctagta cagaaaggca ttgcgcttca ttcatccgtt
     4261 tgcgagggtc tttatcatca tttcgattaa tatttaactc ataccacgaa ttataaataa
     4321 taaaggaacg ccaaggtact gctcgttccc gttcgagata ggcaagaaat gagcgacggc
     4381 gctggccatc agcaatcata ccaactactg aagagacatg aaatgtatca cctattgcta
     4441 atattgtatt gcgattccat ttaccggaaa ttgatgtata attttcggca tttgtatttg
     4501 tttctgattt attgacatca tataacttag tgtttggtgt tatgtttggt gatttcgcct
     4561 caacaaacga agctaacgat atattgtttt gcgaaagtgt agccattggt gtttctactg
     4621 ccgcaaacag acaatttgaa gctattactg accctcgtgt attgccaact attgtaggtg
     4681 ttgtatttcc tgcaatattt tttacagtaa actgtagcgg tgttatgctt tgtaattgta
     4741 tgttttcttt agaatagact gataattcga aacgtaaata atgagaacta tctcgtaata
     4801 ttgcgcgcca taatacggtc aagcttttat attcaaatgc tgcctttaca gccttgcctg
     4861 ctacttgaag tgagcgtttt agagcttgac tattagcttc taaattttct gcttctaaag
     4921 atttgcaaat catttctgat gacataattt ttgtaccatt gcctaagtga atttcaaaca
     4981 gctctgtgcc ttttatcaaa ttcatttcag caacaccgcc aaaaattaat ttgtttcctt
     5041 gttttataaa tgtcgccgtc aacaaattat tctttagcgt ccaattatca ttctgtgttt
     5101 caaatgtagc gcacccaggc tgttgtatag aaggataatt aatagcaact gtttgcagct
     5161 gtattgaaaa aaagagtagg aaactgaata caattcttag actaaaagga aaattatgca
     5221 tagataaaga tactaactaa cattaaaata tacttgacta aaaaaaagac tgctctaccg
     5281 cagtctttta taaatatgaa ttaaaaatat cctttatttc gttgctgctg catatcgagc
     5341 attcacttcg tcccaattga taagattgta aaacgctgca atataatctg ggcgacgatt
     5401 ttggtaatgt aaataataag catgttccca aacgtctaaa cctaagatag gtgtgcctgg
     5461 acactcagcg atatccatca agggattatc ttggttgggt gaggaacata ccgcaataga
     5521 tttatccgct tttacacaca gccaagccca accagaaccg aatcttgttg tagctgcttt
     5581 tgtaaattcc tctttaaatt tatcataaga gccaaaagtt gcttgaatag catcagctat
     5641 tgcaccagta ggctcgcctc ctccatttgg agataagata gtccagaaaa gtgagtgatt
     5701 aaaatgtccg ccgccattgt tgcgaactgc tgcaggcaat gtagaaattt ttgccattaa
     5761 ttcttctaaa gatttacctt cattttctgt tcctgcgact gcagcattta agttgcttac
     5821 atacgcatta tggtgcttgc catggtggat ttgcatagtt aaggtgtcaa aatgaggttc
     5881 taatgcatca tgtgcatacg gtaaatttgg taattcgaaa gccatttttt tttatttaag
     5941 agttattgaa tataatagta aagattgaat gattaattaa ttgcattcaa cctttgcaaa
     6001 tatataacaa atgatagtga ttttagttta gaagcagaat gaaaatataa aaaaatgttt
     6061 atcatgccgt caactcatta atagatattt acaacttcag ttatggtatt tttttacgtc
     6121 acaaatttta ataattattt attctaatgt attgatatat atatatgtat atgtatgtgt
     6181 atgtgtgcgt gtcacttgaa ttcagcaaga aaaatgttag atagtaaaaa tattacaaga
     6241 agtatatttg gaatacaaat tcttgtaatt taataagcgt aaaataaata tgacatgaaa
     6301 tacaaaatac tattaatatt tacgattttg ttgctaataa gcaccttcgc aactcaagtt
     6361 aaaattggtt gaatggacat taaaattagt gcttatgaaa aatattattt caataattct
     6421 ctttatttca tttctatcta ttaagtgtaa aaatgaaaat ataagaccac ctaacaatct
     6481 ggctggaaaa attaaaacac tcattcaata ttctggaata acgacaccaa taataaacag
     6541 ttattcaggt atcaatttat ttatgacagc ataataggca cacttgaaaa aattaatctg
     6601 tctgttaaaa gtaacgatat atattataac aatactaccg agtatattct cacaaatact
     6661 aatgacaata aattgagctt tttatggctg gaagatggtc gaaaattcaa agcatataat
     6721 atatatttta acgacacaaa acaaatttca tcaattaaag aaacagacac tttaggtaat
     6781 aatgaggcac atacaactca tatttttagg cttaataata atatagacag catttttgac
     6841 attggatatg ctggttcatt tatagaaagt ggtacatata tcagtaatat aagttacaat
     6901 aataataatt gcaaatcatt ccttttacaa tactcaacct attatccaac caatacaaat
     6961 gttgatgaca ccatgcagtt ttatttttcg cagcaaccca atacaaacat gttaccttat
     7021 caattacctg atgccaatgc aggtggtagt atttttagat tattgagtta ttacttaagt
     7081 attaatggat attatgcatt taatcctaat attaatttaa ttgatagcat aaaaattgca
     7141 gatgtaacaa tcaggtataa ctacgaattc attaataatt ttgtatctaa ggcacgttat
     7201 attactgaaa gagataatgg cagtagttat acaaataata atatttttga gtactattaa
     7261 agagatatgt tttacgctac tttcaacttc gctaattttg atttacttag tttttgtttg
     7321 gcgtaagctt ctgtaattgt atattcttta acatctgttt tagatggcaa ctcaaacatg
     7381 gcatcggtaa tgatggcttc gcatatcgat cgcaaaccgc gtgctcctaa tttgtattcc
     7441 agcgctttat ctacaataaa atctatggca gcagcctcaa aatgtaaagt aataccttct
     7501 atggcaaaca attttttata ttgttttaat aaggcatttt tcggttgtgt caagatattt
     7561 gttaatgcat ttttatctaa aggatctaaa tgcacaagta ctggcaatct gcctagcaat
     7621 tctggaatta taccaaaagc gcgtacatct tgatgagtaa tataattcag caaattttct
     7681 ttatcaactt cttcgcttcc ttttttattt ttaaaaccaa tgactgatgt attgagtcgc
     7741 ttcgaaatta ttttgtcaat accatcaaat gagcctccac ataaaaacaa gatattttta
     7801 gtatctactt ttaataattt ttgttcagga tgttttcttc ctccttgtgg cggcaccaat
     7861 acttcggttc cttcaattaa ttttaataaa gattgttgtg tgccttcttt aatatcacgt
     7921 gtcaatgaag gattgtcgcc cttgcgtgag attttatcta tttcatctat aaaaacaata
     7981 cctttttctg cggcattaat atcgtagtcg cacacttgca ataagcgtgt taaaatactt
     8041 tctacatctt cgccgacata tccagcttct gtaaacacag tagcatctac aatggtaaac
     8101 ggcacattca ataatttagc gatagatttt acgatgagtg tcttgcctgt tcctgtttgg
     8161 cctaccatta ttatgtttga tttttcaatc tctacatcgt cttccgttgt tttttgatac
     8221 aatcttttgt aatgattata gacagctacg gcaatgactt tttttgcttc atcttgacca
     8281 attacataat catctaaaaa tgcttttatc gctagtggct ttagattttt attgggttcg
     8341 cctttataat ttggtttctc gtgctgtaca aatccttctt catttaataa ttgcatggct
     8401 tgttccacac aagcatcaca gatttttgca tcttgtccag aaatgagcat ctgaacatct
     8461 tcttttgctc ttccgcaaaa atcacactta cttataactt tctttgccat atacaaattt
     8521 ataaaaattc gctttgcaat gctattgcat taatcttatt taatatacag aacagtacgt
     8581 tgtatgcatt tgtagtgcat ccatttattt cttggatgca tgtgatactt cacttcaaag
     8641 aaagtacgga cacatgatag gtgtccgtac tacttttttt ttattttctg attaatacct
     8701 cgtcaatcat accgtagcct ttggcttcct cgctggtcat ccagtaatcg cggtcgctat
     8761 ctttttctac tttttcgaag ggctgtccgc tatggttggc tataatatcg tacaattctt
     8821 ttttcaattt cgtaatttct ttgtacgtaa tttctatttc tgaaacttgt ccttgtgcgc
     8881 cacccatggg ttggtgaatc atcacacggc tgtgttttaa tgcgcttcgt tttccttttt
     8941 ctccagcaca taacaataca gctgccatcg atgcggcgat gcctacacag caagtcgcta
     9001 cattcggttc tacatattgc atcgtatcat aaatacctaa acctgcgtaa acactaccac
     9061 ctggcgagtt gacatatatt tgaatatcgc gtgttctgtc agcagaatcc aagaataaca
     9121 attgcgccat gattacgttg gctacataat catcaatgcc tgtgcccatg aagataattc
     9181 tatccatcat aagcctagag aatacatcta acgcggccac attcatcggt cgttcctcga
     9241 tgatgtttgg cgttagatta gtaatattag tttgtaaata attatctaca taaatagaag
     9301 ataatccgtg atgcttagta gcatactttc tgaattcttt accgtagtcc atgtttatag
     9361 tatttagtat tgagtaatta gtttagaaat gtaaacaaag tcgaattacg caatacgtac
     9421 tactgaaact tgatacgtac tacttctcgt ctttcttatt aaaaaactcg tcaaatgtag
     9481 ttgttttttc ttcaactgta acctgacttt cgataaaggt aaacaatttt tgctccaacg
     9541 caccatcgta tgactttttg acatgttctt cttttgccat catactgtta ttgaagcttt
     9601 caatcatatc atcagtgatg ccgccgccat acatcataaa ctgtgcacga atttgttctt
     9661 tcgaaaaatt cttcacatct tcaaattcta ttttaatatc attttcctta gcaatggcat
     9721 tggtaattaa attccatttc aagccctttt cgaattgtgg atattcttgt tctatttgct
     9781 cgtcatcgat tggcttttca ttgctcgatt tgataaattt ctttaaaaat gcatctggca
     9841 atttcacttc tgttttttcc attaaagtag tatagatatt gtctttaaat ttattgacaa
     9901 cagattgatc agaaaagtcc tgcaattctt tacgtatttt ttctttaaac tcatctacac
     9961 ttgtaacaat attagggcca tacactttat caaacaattc ttgattcaac tctacttttt
    10021 cgtatttcgc ttcctcgcct tctgctctat ctggatttat taaattacca tggcgttctc
    10081 ttagtttttc tacttcgtct gttaaagttt tctcatctat gctgattaca tctttaacga
    10141 tgcttgtttt tttagaaagt gcatctactt ttacctctgg tgctaatcca atttcatatt
    10201 caaaagtata atcaacagga ttgttaatgt caatttctaa agtctctgat ggaattggca
    10261 atggacgacc caacatattt aattttgaat cttcaatatg tttattcaag ttttcagaaa
    10321 ctgctttatt caattcatct aacaagatgg aattaccata catcttttta attaatgaag
    10381 ttggcacatg tccttttcta aaccctttta ttgctgcatt acttttagcg tttgctaaag
    10441 ctttttctac atttggaaga taatcttctt tctttaattc gatgcttatg atggcattca
    10501 aactgtctgt gtttttttga gaaatattca cttgttgtaa attttagtta aataaaaaat
    10561 gttgccagat tgaacaagat tcttcacttc gttcagaatg acaacattca aaatttgtgc
    10621 gggtggaggg actcgaaccc ccatggttgc ccgctagatc ctaagtctag tgcgtctacc
    10681 aatttcgcca cacccgcagt ttcctgctca atttgggttg caaagataag aatatttcgg
    10741 caaatcacca atctgtaaat taagcaatta taaaatattc gtatctttgc gccattatga
    10801 aaaatatctc tctcatctta aacgtcattt tattcatttt agtaggcgtt ttattctatt
    10861 tacagttcac tgaaaaaaag ggctctagtg ctgtacctca agtgattaaa accgatggca
    10921 agtctgtaac cgtacctcaa attgcctata ttgatatcga ctccttccaa gcaaattacg
    10981 actactttaa aactggaaaa acaaaattag aagcgaaaca aaaatctatg gaagccgaat
    11041 tggagagaag catgagcgcg ttccaagcgg aatataattc attagtgcaa aaagcacaaa
    11101 ccatgacgga agaagaaggc atggcagcgc aacaaaagtt agctgaaaaa cagcaaaaaa
    11161 ttgaagagcg caagcaatct atggaaatgc aattcatgaa agaaacttca gattttaata
    11221 tcgaattaca agaaagaatt atcgcctatt taaaaaaata taatgctaat ggtgcctata
    11281 cctatattct accctattca agagaaacga ttaatctctt gtatgtaaat gatgcttaca
    11341 atatcactac tgaagtatta aaaggcatga acgaagaata taaaacaaac aaaaaataag
    11401 ctgtaagact tatttatatg taaaggttca agtaaaatac ttgaactttt ttttatctta
    11461 gtttttaagg tagtatattg tatcctaatg gaagtaaatg tagaagcaga aaagcaagcc
    11521 attattaaag attatcgcca attgttgcga ctcgcaagac cgcgcatgaa aaaaggcgac
    11581 gacaaacgca ttcgccatgc atttgaatta gctgtagatg cacacaaaca catgcgccgc
    11641 aagagtggcg agccttatat tcaccatccg attgctgttg ctaaaattgt tgctggtgat
    11701 atgggcttgg gcacaacttc tgttatctgt gcattgctac atgacactgt cgaagacacc
    11761 gatttaacga tagatgatgt aaaaaaagaa tttggcaata cggcagcaaa aattattgat
    11821 gggcttacaa agattagcaa aatatcgcac caaacagaat ccattcaagc tgagaattat
    11881 cgcaaaatat tattgacttt aagcgatgac attcgtgtaa ttctgataaa aattgccgat
    11941 cgattgcaca acatgcgcac gctcgacgga caaagccgga aaaatcaatt aaaaatagca
    12001 tcagaaacca tttatttgta tgcaccattg gcacatcgct taggtttata ttctatcaaa
    12061 ggagaattgg aagatttggc tatgaaatat accgaatcgg aaaagtataa agagattgcc
    12121 aaagcattag aatctaccaa aaaagacaga accaaataca tcaatgattt tgtgcgacca
    12181 ttgcaagaag aaatggataa atctggtttg aaagcgaatg tttttggtag accaaagtct
    12241 atttcttcca tctggaataa gatgcgcaaa aaagaagtag aatttgatga tgtatatgac
    12301 aaatttgcca tacgcgtaat cttagattct gctcaagaaa atgagaaagc agattgctgg
    12361 cgtgcctatt cgattgtgac agacaaatac tcgccacaac ccaatcgtat gcgtgattgg
    12421 atttcgcacc cgaaaggcaa tggctatgaa tcgctacaca ccacagtaat gggctctggc
    12481 ggacagtggg tcgaggtgca aattcgctcg caacgcatgg acgaagtagc agaaaaaggt
    12541 ttagcagcac attataaata caaagaaaac aaatttaaag cggcgaattc ggcggaagaa
    12601 gctattgaaa gttggctgat gcaagtacgt gaatttctaa aaaatcctga tgtcaatcca
    12661 attgagttta tcaatgactt caagcttcag ttgtaccaaa aagaaattta tatttttacg
    12721 ccaaaaggcg aattaaaaat tttacctgct acggctactg cattagattt tgcttataca
    12781 gtacacacta atgttggcga caattgcatc ggtgctaaag taaataataa attggtgcct
    12841 atcagccatg aactaaaaaa tggcgaccaa gtagagatta ttacatcacg caagcaaaag
    12901 ccgagtgaag attggttgaa tattgttaca acatcgcgtg ctaaatcaaa aataaaaaat
    12961 tctttgcgcg aggagaaacg cagaattgct gacgatggta aagaaatatt agaaagaaaa
    13021 ctgagtcaaa taaaagcgaa tttcagcaat gacaatatca acttgttgac taatttatat
    13081 aaaatgccaa gttctatgga cttgctgtat ggcattgcta aaggcaccat caatcttaca
    13141 gagttaaaac aattacgcaa tgttggtggt gctttagaaa tcattaaacc tgaaaaaata
    13201 ggcaaaccca aagacgaaga agttttttat aaaactgaag acaataatgg tgatattcca
    13261 aagaatgcgg agttggttat ttttgatgag tcgtcaaaat ttccttatga gtttgcaaaa
    13321 tgttgcaatg ccattccggg tgacgatgtg attggattta tatctatcaa tggcgtgata
    13381 aaaatacaca gaacaggctg tccgaatgtt attaatttag catctaaata tggatatcga
    13441 attataaaaa cacgatggac tgccaagacc acacccgcat ttttaactac agtaaaatta
    13501 actggcttag acgacatagg cgtcatcagc aaacttacta cgattatatc aggagagatg
    13561 aaattgaata tgcagtccat ttcattagaa accaatgaag gtatttttga aggtatcatt
    13621 aaagtatttg taaaagacaa tgagcaagta aataaactca tcagcaaatt aaaatctatc
    13681 catggaattg actcggtgat tagagaagag atttaatgct gtatttgctt tttatgatat
    13741 ctttctaaga aatttttcgt catttgagtg aaaaccttat ctttattcaa accactacca
    13801 caggcgattg aaccaaaaga aagaattgca ttatttttat tttcaattat tagaatatca
    13861 ccaccaccat tatctggatt tgttcctttt gccaatagta cagaattttt aggtgtattg
    13921 tcatttactt tatccatctc atcaccgctt aaacctctgt tgtctatacc atatttgcca
    13981 aattcatctc catttttaat agtacaattt ttatacagcc aatgttgtgc gtttattact
    14041 ttataactac aatacgtttc gtaaccagca tctgtataag cattgcctaa atattttgct
    14101 tcattagaga atgatgtacg ccataagcct gctggaatta aagtattctc aaaaaaagtg
    14161 ccatcttttc tacattcaat tttttgataa ttatcagaaa atctaatttt ccaatatgcc
    14221 tgatttccac ctaatgatat tatgttttta gacttagaaa actgtgccaa tgcattaaac
    14281 atttttgttg aaaaatactc acaatgctga gcaaatatta ttgtttttgc atttttgaat
    14341 aattcaggat gtgcatctaa ataataatca ggatatacat tgcattgata attttgaaag
    14401 aaaaatttgt aaatattatc aaagataaac aagtcgtgtc ctactaaaat cgaatcaaat
    14461 gtgcaagaaa ccaatggacg ttgtgtggaa attgtatata cacaactatc gtcttttgta
    14521 tttacataga aactttttcc accataataa ttatacgcat gccaagtcgt gacaggtgca
    14581 agtaaaacta tatcagattg ttgattacta ctaatgatga acggaatatt aaaaactgat
    14641 tgtttatctt taagttgtat acagtatgtg tttgggctta agtcttttgt ttcaatgatt
    14701 atattttctt tatttaaaac tgcaatatcc attaattttc gaaacttata attttctatt
    14761 ggttgcagca actgtactga atttagctga tgcgcatttt gtatcttaat ggtcacctgt
    14821 tcacctttag tataaataat tttatctgtg taacctgata attttgtttc aaatgtttct
    14881 cgtggttttg tatttacact tggatttttt aaaatatttg catcttgttt tatcatatca
    14941 aaaatatcat tcaatttaca tttttttgtt cttctattta attcctttat gtattccttt
    15001 ttaggtataa attgtttgtc aaaaacagaa aataaaatat cttcatctaa taaatctaag
    15061 aagttttcca ctgaaacatt ttttccataa aatgctaact catttagtaa gttatatgta
    15121 agtggtttaa aaataaaatc aggtacacta tcttgaaatg gaaattcaga gtactccgtc
    15181 acttttaaac tatcaacata agccgtatcg tttgtccagt tataagcata tattttcatt
    15241 atgccttgtt taatagtatc aggtgtttta ataaataaat ttatataata ccattccttg
    15301 cttttatctt gaaatgtagg gttatgtgaa ccaatccagc cataaaaatt agattctgta
    15361 gctaaattaa ctgaataatt ttttgaatgt acttttaatt gcaggtggta atatgttttt
    15421 ggcttaacat ttttttctat tgtaaagcca aaattatgct tgggtgaaag ctgaattgct
    15481 tttgtgaatg cattgtaata tgcaattgta ccaatatcta ctagtttgaa attactatta
    15541 ttacttttgt gattttgcgt tgtaagaata aatgaattaa atgattgttt atgagtgcaa
    15601 cttatcatac aataacataa agatattgaa ataaaaaaat gcaaatattt catagctcac
    15661 taagattctt tttctaagaa tattatttaa aattattagc ctattggcaa gaaattattt
    15721 caggtattac ttcaaaagtt gctcctaaag gtacttcaaa ccccggattt aagttagtag
    15781 tttctgcaat aaaagttaca tcttggttta atggtacact tgtagatgta caaccagttc
    15841 caggtgagtt aattgtagta gcaccatata tctgaggatt attagtaaat gagcatacat
    15901 ttaatacaga aggtctagta cctacccaaa ctcgtttagt gataaatgta ttgccatata
    15961 caccaaaacc tgtggtaaat gttaagattg caccaccaga tgaagcagta gaagcaggtt
    16021 taaagtttat tactggacct ccacaaatat ctgcattttg aaataaagta cttggatatg
    16081 cagttgcatt atcattaact cctaattgta aattattaat agtataattt gaaccgtttg
    16141 gattacaaat acttgtaggt ccgcttacta taggtgttgt aacattgaca ccactgatta
    16201 tacaatctga tatattgcct tgattaccta ataaaaaata atgcacagta ttaatttgac
    16261 atcttgtcaa tgcatcttgg gaaacattat aatccatagt gttatttgaa tttggattac
    16321 aaccaggctg agtattatcc caacagtttg gattatttgg agtatcatta cagttatcac
    16381 ctgaccaaga atgatataat cctaatgcat gacctaattc atgtctaagc aagccactag
    16441 cattccacat tgtattattg agatatgctt gataaatact attaactagt acccattctt
    16501 tagctccaaa ccatttagcc tgtccacctc catgcccatt tacatttccg cctaaccctg
    16561 agataaatac atgtaatgta gtatatttat tttttacacc atgcttattt acaacataag
    16621 ttgtgtataa actatctcct ttttctgaac ctgctggcac ttggttatct attgtgccat
    16681 cccaaccaaa agtatcattc cagaaataaa ttgtatctaa tacaaattgt attttactat
    16741 ctgcaatatg tggtgaagaa gttggaagat tcattacgga taaattattc attttgttat
    16801 ttatatagaa gtaagtaaaa tcgtgtaaca tttgcctgtt tgtaggatta ttaggtatat
    16861 tacctgttcc gtcattttta ttaaaaagat gatacgcaat tcttacgtat tttatatttg
    16921 tattatcgtt caacgttgta tacgtattcc agtcattaca aatacttagt aatgaatttt
    16981 gattattaat agatagcctt tgcggattat taactaatgg gcatcctgat gattgaccaa
    17041 aatttaactt tgaaatcaga actattagtg ataaaatgaa aatatattta tatatgcttt
    17101 gcatgataat atttttagtg gttaacaata atttttttta ttgtaaattc atcatcaata
    17161 gagattttca agaagtaaat gccatttaaa acatcagaaa gcgaaatctc tttaaattta
    17221 agcatattat ctattaaatt aataggcttt attattttgc cattaatatc taatattata
    17281 atttcagctt tttggtaatt ttttgttcca tagtattcta taagtatttt atcagaaatc
    17341 ggatttggat aaatttctat ttgtaactga ttattgtact tttcttttat caatgctaaa
    17401 atagaatcac atgcaagaga actaaaatta atacttaact cagtatcttg ataacatctc
    17461 aaattcagaa ttggtggttc agttgaaaga aaataacttg aattagtaaa accccaaaaa
    17521 catgcttttt ctttaatttc taaatgatat gagggaaaaa ctgtagttgt tttaggcttt
    17581 aagattccat attgcacttt aatagttgaa ttttgttgta ggggtctaat gctgtcgata
    17641 atataaaaga aagaatctaa tatattactc gaattaggtg cgacaaacaa acaagtatca
    17701 tgttcacttt ttgtaaaatc acaaattggt agcaattcat tatttagcca tctatagact
    17761 ttattgtttt cttctttaat aattaaacgt ctaacactat ttgtatgata atttacttga
    17821 tttaaaattt ggaaagtatc aataccaata attgtatctt tttcaacaac ccatttttct
    17881 gtatatggtc catctggaca acacaatgta taatcccaag accactttgc accaatagga
    17941 aaccaatctg ttgcttttag ttgtgtaaaa gttataatga aaaatgatag tagtaataaa
    18001 ttctttttca tcttgaattt atttactagt taatatcatc ttttttgtag caatttcttt
    18061 tccatcgcaa ataagtgtgt ataaatacat ccctgctttt aggctattac catcaataaa
    18121 aatgcttcct tttccgctct gatttattga aaaactactt aactcaactc cgttcaaatc
    18181 ataaatatgc aaactactat ttgtacaatt attcggaatt gtatagctta tttgcgtttg
    18241 ttggtgaaat ggattaggat tattttgctc taaaaaagca taagtatttg gagtattttc
    18301 tattatttta tttctttcag tttcactttt ttcggagtca tataatttgt ttgtgcaaca
    18361 ttgttgaagt tgtctttgta agtcttggat ttttttattc tgttctttaa ttgattggat
    18421 taaatatggt attagaccta tatagttaat tcctaaatac cctttattat cttcagatat
    18481 taaatttggg agaactgcct ttaattcttg tgcaacaaga cctgcttgat tatacaatat
    18541 ttctggtttg attttatcct tactattatt tgtttttttt aactcatctg cgatagattg
    18601 atcattaact ggtattgtta agtcatacgt tttgggtgtg agctttaata ttaaatctaa
    18661 agcattcttg atttcaatta catttgattt tagtcgttca tcagacgagt ttataaatcc
    18721 ataacatctt aaagaaccat taacatctaa tctgtacgaa ggatttccag tatttatacc
    18781 aacataacca tttgcatctt taataaatag cttgaagttt ccataatttg ttgacggata
    18841 tggtttccaa atattaaatc cacttccatc tgtatcaaaa gcccattcag caggtgatgt
    18901 aggataattt tgagaacgag tgaatttaat tccctcagaa tatgaaacac ctgttttggg
    18961 tcctaacagt aaaacatcat taatatgtaa ttttccttga ggtgagctaa caccaagtcc
    19021 aacatttcca tttgaaatta cttttatttg tgcatttgat gtagtactac taaacatcat
    19081 ggttaatagc attaatacgc ttataaactt aattgtgtgt gtgtatgtgt gtgtgtgtgt
    19141 gttagaaaag aattttgcat attgaagtat ttttatgtaa aataataatt aatttatgat
    19201 attgtcaagt attagcataa aaaatattta ttaacaaaaa atgtacctgt aaaattcacc
    19261 tcatatattt cagttaaatt tcttttgact ttgctggctt gtagcctttg cctttgatag
    19321 cttgtagcca acaatcagcc aaacaataat gattaccgtt tatgacccat aactcttgca
    19381 gtatgcagcg cagcggttta taagcaaaga actacccatc gaaagaataa t
//
LOCUS       DAOQQV010000040        19363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_853_length_19363_cov_7.231821, whole genome shotgun sequence.
ACCESSION   DAOQQV010000040 DAOQQV010000000
VERSION     DAOQQV010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19363
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_853_length_19363_cov_7.231821"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..982)
                     /locus_tag="PLL59_05860"
     CDS             complement(83..982)
                     /locus_tag="PLL59_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMY23365.1"
                     /translation="MFQLPSPLQKIENEWTKKFDIQLYIKRDDLIHAAVSGNKWRKLK
                     YNLTQAKYENKNTILTFGGAYSNHITATAKACELLGFNSIGIIRGEAYFPLNKSLQFA
                     ADCGMQLLYLDREHYRNKNISPLLEQIETNEAAIFIVPEGGANEYAIQGCTEIVDEIN
                     QQLMENYSTKNFDFICCASGTGTTIAGISMNLLAHQTAIGFSVLKHDTLKQEILSRIP
                     NLKDNFSMQAAHFGGYAKTTPALLQFIRDFYKTNNIILDYVYTGKMMYAFFDMLSKGF
                     FPSKSTIVVVHTGGVMNANLFEE"
     gene            complement(985..2013)
                     /locus_tag="PLL59_05865"
     CDS             complement(985..2013)
                     /locus_tag="PLL59_05865"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMY23366.1"
                     /translation="MDSISQIALGAGVGELVLGKKIGNKALLFGAIAGTIPDLDVFLH
                     FFTHLSESEALLVHRSYSHAAIPQLAIALPFTYLSYKLFKKQISFFRWYLLWYLGFLT
                     HAMLDCCTTYGTQFFLPFSRYLVGLNNISVVDPLYTIPFLILLAILLFYPKESKTRFI
                     IAVLSVVLSTGYIGYATLNKFKANSFFKDALHKENIQYTQFSTSPTLFTSFLWNMIAA
                     NDSTIYVSEYSIFQSSQKPIIQAYKRNLHLLDDCKNSEAVKTLLWFSQGKYFVEKGNN
                     DTLDFYNIKWGRTDYTQINPKDAFRFYVKIYKDKNGIYTFDKVEPNFKRRDIKAYLSL
                     IWHRIFDN"
     gene            complement(2056..4644)
                     /locus_tag="PLL59_05870"
     CDS             complement(2056..4644)
                     /locus_tag="PLL59_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HMY23367.1"
                     /translation="MSTEEFNEQHSDHITTIDGLYENWFLDYASYVILERAVPALYDG
                     FKPVQRRILHAMKEMDDGRYNKVANIVGSTMQYHPHGDASINDAIVNIGQKDLLIDCQ
                     GNWGDIRTGDSAAAARYIEARLSKFALDVVFNDDTTDWQVSYDGRKREPVQLPVKFPM
                     LLAQGAEGIAVGLSTKVMPHNFCELIDASIKYLRKEPFELFPDFLTGGRVDVREYNDG
                     KRGGRIRVRAKMEIVDKKTLRITEIPFGTTTSDLIDSILKANEKGKIKIKKVVDNTAK
                     EVEILIELQPQVSPSVTVDALFAFSDCELSISPNTCVIIDDKPHFIGATDALKLSTDT
                     TVHLLKRELEIQLNELEDKWHFSSLEKIFIENKIYRDIEEEETWEGVIAAIDKGLTPF
                     KKLLMREVTEADITRLTEIKIKRISKFDKKKADEYIKGLEDEMEETKKNLKSLTKYAI
                     KYFEDLLKKYGKGKERKTEIAIFDTVEIKTVAANNEKLYVNRAEGFIGFGKDLKKEEF
                     IAECSDIDDIIAFMKDGTMKVVRNAEKVFVGKDIIHVAVWKKNDERTTYNAVYLDGKS
                     GTSYAKRFNVTAITRDKEYPITQGNPKSKILYFSVNPNGEAESCSVVLSPNCSARNKV
                     FEFDFYNLDIKGRSANGNIVTKYPVKKIEQKGKGRTTLGGVEIWLDDTVGRLNIDKRG
                     RYLGSFQNDDRILVVLKSGEYYLTNFDLSNRYTMNDIVAIDKLYPTTTISAVHYSDSK
                     KANYVKRFQIETTSIDTKNNFISDENNAKLVFATLFSNPTIEYTVDLGKGKTSEPSTI
                     NLLEFIEVKGWKAIGNKLIGGTIKDIRLIESATNEAEEQAVNFDVDFEITNLNDKKEP
                     EQGELF"
     gene            complement(4672..6534)
                     /locus_tag="PLL59_05875"
     CDS             complement(4672..6534)
                     /locus_tag="PLL59_05875"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HMY23368.1"
                     /translation="MAEVQYTEDNIRSLDWKEHIRLRPGMYIGKLGDGSAPDDGIYVL
                     LKEIIDNSIDEFVMGHGKQIDVSIKDKTVTVRDYGRGIPLGAVIDCVSKINTGGKYDS
                     RAFQKSVGLNGVGTKAVNALSSYFKVESVRDGDIKVAEFDKGVLMQDHKIAKTTDRNG
                     TKFQFHADNTIFKNFHFVTEFIEEQLWNYAYLNAGLTINFNGQKFVSKEGLKDLLNKK
                     TNEAELRYPIIHLKDNDIEIALTHNNQYGEEYYSFVNGQYTTQGGTHLAAFKEAIVKT
                     LREFYKKQFEASDIRQAIVGAIAVRVQEPVFESQTKTKLGSLNMWENGPSLKSFVNDF
                     IKDKLDSYLHKNPTIADALLKRIQQSERERKDLSGIRKLANERAKSANLHNKKLRDCR
                     VHYTDEKNELHADTMLFITEGDSASGSITKARNPQTQAVFSLRGKPLNCYGLTKKIVY
                     QNEEFNLLQHALDIEEDVDNLRYNNVVIATDADVDGMHIRLLLMTFFLQFFPDLVKKG
                     HLYVLNTPLFRVRDKKETIYCYSEQEKIQAIDKLRGKPEITRFKGLGEISPNEFEQFI
                     GDNIRLEPVVLKSNQSIEQLLEYYMGKNTPDRQLFIINNLKVEKDEVAAELVEN"
     gene            complement(6673..7332)
                     /locus_tag="PLL59_05880"
     CDS             complement(6673..7332)
                     /locus_tag="PLL59_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMY23369.1"
                     /translation="MRRLFSIISFYIFMLVSAYAEVGVSVGAKAGFDVAFIRKYPSPY
                     TYSKRAILGSDISAVLRIDFNKFIGLQTELEFIQKGQGWKNSNDSAKFSAKKVLNYVQ
                     FPILAVARVGSQKVKAVFFLGPYLAYQTGGYLQESVSVDKQSKNSITSKYLFSPTDNR
                     FDVGMIIGAGTNIKVGKGWIEVAARQDIGMMPLVKLKSGLPKTYNCNFNLSIAYLYTI
                     K"
     gene            7474..8058
                     /locus_tag="PLL59_05885"
     CDS             7474..8058
                     /locus_tag="PLL59_05885"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMY23370.1"
                     /translation="MKKLFLALTITASTFGAFAQTTPAVAPAVDPNAPTMEFTKDAID
                     YGTIKQDADGNRTFSFTNNGTTPIIISEAHGSCGCTVPTYPKEPIMPGQKGEIKVHYD
                     THRVGPFSKSVTVNSNAKNSPVVLKISGTVEAAAVVPTATEPSIIPAPAPATTTPAVK
                     ATTAPVKKTTAPVKKTTAPVKKTTSTTKAKTTTK"
     gene            complement(8112..8867)
                     /locus_tag="PLL59_05890"
     CDS             complement(8112..8867)
                     /locus_tag="PLL59_05890"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMY23371.1"
                     /translation="MAKIEIPTTQHVIIQYETAGFLWRAIAWSIDFFIFNTVIFLALN
                     FMPNWLSSLLGLSFSFFELLAYFILYFSLFILVQLFIEKRLNGQSIGKKVVGIKIISL
                     DGNSMDFYKYFVRCMYNYFDIYFSFFSVGSLLILMSEKNQRLGDMVTNVTVVRLKPDR
                     VVTLTDLLNLPDKDEYIPKYPQVVHYTDKEMLALKTLLIRTQQFNTDVYQILLHDTVE
                     QLKQQMDILVQDSNQSDADFLREIISEYVILTR"
     gene            8989..10845
                     /locus_tag="PLL59_05895"
     CDS             8989..10845
                     /locus_tag="PLL59_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HMY23372.1"
                     /translation="MKESKFIEQNQKKWAEFEEMQNNKHKDNNHYSKLFIQITDDLSY
                     ARTFYSNRSVRLYLNKLAQDIFHKLFSKNKKQKSFFKDFWLEELPSVMYYHRTELLVS
                     FTTLIIAIGIGLLTYMHDNEFAAKILGDTYLQMTAENIAKIDPMAVYKSMSPVQMFNH
                     IVINNLRVDIITFFFGIIFSIGSIFVLIHNGLMLSAFQFYFIKQGLFWESFLAVWLHG
                     TLEISAMVICGAAGIVLGKGLVFPGTYSRSQAFFSSAQRATKIFIAVLPITIVAAIVE
                     AFLTRYTETPDVIRFIFILFSLGFILAYFVYLPWLKNRNGTLRAPTNSIILAEKHAAF
                     DYSSIKNISTITLETFSMLRKYSHQFASIIFWSCIIMLFIGFYYNQEYILENSEQDWK
                     FNMIFTFTDNIRLNAFCYVYPILFIVIAFLCNYLFCVEMQIKNKQEKETIMQWLLKNK
                     AAFIICVIYQLAYYYILFNRETNTSTILFALPFQFMVSYVLFFPRLQKENFVFTTFRI
                     TVTGLLKIMVLNFLFIAFICLLYALLNSSVYWIIYEFVNTNLTDNVSTRDKAYLLLEI
                     ITVHSIIAIASIIFCMYQGIFYFSQKEKISAEVLKAELENIKIAKNKNVLFK"
     gene            10829..11545
                     /locus_tag="PLL59_05900"
     CDS             10829..11545
                     /locus_tag="PLL59_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23373.1"
                     /translation="MYYLNKIFILLILIKCNTIVAQQNNNIDNESNIEKQDKYLHQHI
                     DKKEFDKHKWETLREKTKIDESFQKRNQEAAQNNKPAQNKAISGTLENIIKWTVYIII
                     FGILMYLIFRAINFSIFNKNKKLKTKFSIEEIENDLNNAEIDPHLYAAIKSKNYKLAI
                     RLYYLKIIQKLHEKEKIIWRKHKTNKHYLQEMYDKEDYIIFKEITQQYEKYWFGNTTI
                     DETKYNTLSMEFVNYHQNIK"
     gene            11546..12847
                     /locus_tag="PLL59_05905"
     CDS             11546..12847
                     /locus_tag="PLL59_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23374.1"
                     /translation="MSKDSKKYILVFMAVIIVIIAYFLLRDYNEKWNLNFDKTKKNAY
                     GTYITYEILKHNNDSNFIEINNSVISTFRTLDKNKKYNYVFINYANFYDSATIDTICK
                     FVENGNTAFLAVEDIYYSRILSDSILEQQYHLRTNTDLFSESDTIVKDKNSARFNFIN
                     PSLQNKDGYCYYVRDEYDTIQYAFNHFEAIPDSEWTATNNKEKELLLFGNKTVNHITN
                     NFISLKHGKGNFIILLSAIPFTNYFMRKQEGLEYAEKVFAHLPNNTIIWDNVSQYEKE
                     EINDDRINDSRYNESALYFILNNRSLRWAWYLCLLGVLIYVIFQAKRRQNIIPLIETK
                     QNTSLQYVQTIGQLYYQDKAHQEIAKEMKLQFLNFLRNKYNCKTNEIDKVFMQQIAIK
                     SNIDKEKIASIFNHFNNIETVNTTQLHQLNTALEYFYKNCK"
     gene            12852..13838
                     /locus_tag="PLL59_05910"
     CDS             12852..13838
                     /locus_tag="PLL59_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMY23375.1"
                     /translation="MEENKPTNAVASEDINFVLSITEKIKNEVGKIIIGQNSTIELLL
                     AALYVNGHILLEGVPGIAKTLTAKMLAKTIAVDFSRIQFTPDLMPTDIVGTSVFNMKN
                     TEFTFKQGPVFSNIILIDEINRSPAKTQAALFELMEERQVTVDGTTYPMEYPFLVLAT
                     QNPIEQEGTYRLPEAQLDRFIFKIEMKYPNLNDEIAILQRFNYDFSQTITQDVSAVAS
                     ANDIKKCSNIIEKIFIKDELINYIANIVYETRNHGDLFLGASPRASLSLLKASKAMAA
                     IAGRDFVIPDDIRAVADAVLNHRVIPSAEKEMEGITAKDIIADIINKIEVPR"
     gene            14083..15213
                     /locus_tag="PLL59_05915"
     CDS             14083..15213
                     /locus_tag="PLL59_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMY23376.1"
                     /translation="MPNILSLGDEQNITLRIKNKNLSPIKIEVIDELPIEFQKRDFSF
                     KLNMSGNETKNILYNIRPLTRGAYTFGNCNIFVSTLLGLIQHKQIFALQTGVPVYPSI
                     IQLKKHELQLFNKLHSFQGIKKSRKIGHSYEFEQIKNYASGDDYRTINWKATSKKNQL
                     MVNQFGDEKSQQVYSIIDKSRAMIMPFNGLSLLDYAINSTLVISNTVLHKDDKAGLLT
                     FNEKMGNFVKADRKIGQLKRITEVLYKEEESSYEANYELLYDAVTKLIKQRSLLIFYT
                     NFESYYTLERYLPVFRKINKTHLLLLVVFKNTEIEKYSYETATDTLSIYQKTIGKKML
                     AEKEMMLKELAKYGIQALLTAPQDLSTQTINKYLEFKAKGWI"
     gene            15350..17170
                     /locus_tag="PLL59_05920"
     CDS             15350..17170
                     /locus_tag="PLL59_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMY23377.1"
                     /translation="MNTTRNWILLTLFTLLSFFTVRWLANYQEKKNTITEPTVDVDID
                     AIKERGTLRVIMEYNSISYFIYKGQRLGFEFELMQHFAKAIGVKLEVIIAKTTDEQFN
                     LLNEGKGDIIANGIIQNSKIKQQVALTQAYRDVEQVLVQRRSGISVNPIDSIQKNDTL
                     LQNVNELSNKIVYVADNSSYYTSLKELSDSSGINMDVRILPDERGVDDIIEAIANGEI
                     DYTIANKDIALLNKSYFENLNVDVAVGKKQALHWAVRKNAPDLLSSINYWLTKFEKGK
                     TFSALYYKYFNEEKNVAYGYNENAELQKGMISHYDQLIQCYAKKINWDWRLVAAIIYQ
                     ESKFNPNARSWCGAQGLMQLMPGTARQVGVYSSEVYNPESNVRGGTEYLRQLEKTWSH
                     ITDYTQRIKFILASYNAGPGHVLDAARLAEKNGYPKDKWDGSVEYFILYKSNPRFYND
                     PVVKYGYCRGQEPFNYVRNIIKKFFDYETKINTATASATPMDMRLEQVESIPFDGIEG
                     AYNPTQGLIARSARRELFLSRKLFGEETQLIPKNSNRNPFDKPKKELFIRENPKEFAD
                     SSRQLFKKKKQLFQVQSPSNSSNLQLNENEEKINQLTPRR"
     gene            complement(17179..18243)
                     /gene="lpxB"
                     /locus_tag="PLL59_05925"
     CDS             complement(17179..18243)
                     /gene="lpxB"
                     /locus_tag="PLL59_05925"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HMY23378.1"
                     /translation="MKYFIIAAEVSGDMHSSQLVKHIQAIDKDAVFIGVGGPALQAAN
                     VKLIFGLERVAFMGFFEVVKNIFTIWRNFKEVKNSILQTKPDRVILVDYAGFNLRIAK
                     WCKQHHIKVAYYISPKIWAWNESRVEIIKKYVDVMLCVFPFEIPFYQKHQYTTAYFVG
                     HPLQDSIAKIVNESSSTAKNHIALLPGSRRQEIKALLPMMLATTNDFPNEKFVIAGMS
                     AVNELYQIPLPNNVSIVYDATYDVLQYAKAAIVCSGTATLETALFSVPQVVVYKTSWI
                     NYQIGKRLAKVRFISLPNLIVDNLLVKELIQDDCTKENCSQALQLLLQQQSTEFYASL
                     QAKMQRPTLTDSGAQLIVRL"
     gene            complement(18261..18584)
                     /locus_tag="PLL59_05930"
     CDS             complement(18261..18584)
                     /locus_tag="PLL59_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23379.1"
                     /translation="MQSNSTSQTTWLDKLDTTLNGFILGMVLPMIMFVIYREIKFSYL
                     PWDQYVASVKDLSVLPSFIKVCVFINLPFFFLFNLLKKFSLCMGIFVASLLYIIAMFS
                     VKYLL"
     gene            complement(18638..19036)
                     /locus_tag="PLL59_05935"
     CDS             complement(18638..19036)
                     /locus_tag="PLL59_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HMY23380.1"
                     /translation="MSVVKDSHKHKHYSEQFTIFLSVLCTIHCIITPILVVVLPVAGA
                     YFEQYHWVEYVIIASVFLFGTSSILHGYKHHHHNKIPAFIFFSGLVLLCAATLLKLAF
                     QVGDFQTHLISGIGGIAAGIGQLYNLKLSK"
     gene            19103..>19363
                     /locus_tag="PLL59_05940"
     CDS             19103..>19363
                     /locus_tag="PLL59_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HMY23381.1"
                     /translation="MSKIEEILKNHDLRITQIRVDILNYFLKDKSNALTHANLEQQFG
                     KKHDRVTIYRTLNSFLENGLVHKIADDSGVAKFAVCQHGSSKH"
BASE COUNT         6764 a         3317 c         3029 g         6253 t
ORIGIN      
        1 tattgacttg aagcactatt ctttgctcca tttttgacca cattgtcttt ttttatgtat
       61 cagttagaag aaaataatta agctattctt cgaatagatt agcattcatc acaccgccag
      121 tatgcaccac aactattgtt gatttcgatg ggaaaaaacc ttttgatagc atatcaaaaa
      181 aggcatacat cattttgcca gtgtaaacat aatcaagtat aatattgttt gttttataaa
      241 aatctcgaat gaattgcaat aaagcaggcg tcgtttttgc atagccacca aagtgtgccg
      301 cttgcattga aaaattatcc ttcaaattag gtatgcgact tagtatctct tgctttaagg
      361 tatcatgttt taatacagaa aaaccaatcg ctgtttgatg tgctagtaaa ttcatcgaaa
      421 taccagcaat ggtagtaccc gtaccgctag cgcaacaaat aaaatcgaaa tttttagttg
      481 aataattttc cattaactgc tgattaattt catcaacaat ttcagtacag ccttgtatgg
      541 catattcatt ggcaccacct tcgggcacta taaaaattgc tgcttcattc gtttcaattt
      601 gctcaagcaa aggagaaatg tttttatttc tataatgctc tctgtcaagg tataacaatt
      661 gcatgccgca gtcagcagca aattgaaggc ttttatttaa tggaaaataa gcttcgccac
      721 gaatgatgcc aatgctatta aaaccaagca attcacacgc ttttgcagtt gctgtaatgt
      781 gattggaata ggcaccacca aaagttagaa tggtattttt attttcgtat ttggcttgtg
      841 ttaaattgta ttttaatttt cgccatttat taccagaaac tgctgcatga attaaatcat
      901 cgcgtttaat gtagagttga atatcgaatt ttttagtcca ttcgttttca attttttgta
      961 aaggcgatgg tagctgaaac atgattaatt atcaaatata cgatgccaaa ttaaggaaag
     1021 ataagctttt atatctcgac gtttaaaatt aggctctacc ttatcaaacg tataaatgcc
     1081 atttttatct ttatagattt ttacataaaa acgaaatgca tcttttggat ttatttgtgt
     1141 gtaatccgtt cgaccccatt ttatgttata aaaatcaagt gtatcattat tgcctttttc
     1201 cacaaaatat ttaccttgac taaaccacag taatgtcttt acagcttctg aatttttaca
     1261 atcatctaac aaatgtaaat tgcgcttata cgcttgtatt atcggctttt gtgatgattg
     1321 aaagatggaa tattcagaca cgtatattgt actatcatta gcagctatca tgttccataa
     1381 gaaggaagta aacagcgttg gcgatgtaga aaattgtgtg tattgaatat tctctttatg
     1441 caaagcatct ttaaaaaatg agtttgcttt aaacttattt agtgtagcat agccaatata
     1501 tccagtactg agtacgacag acaaaacagc aataataaat cttgttttac tttcttttgg
     1561 ataaaatagt aagattgcta gcagaattaa aaatggaatg gtgtacaatg gatcgacaac
     1621 agaaatatta tttaagccaa ctaaataacg tgaaaatggc agaaaaaact gcgtgccata
     1681 cgttgtgcag caatcgagca tagcatgtgt aagaaaacct aaataccaca gtaagtacca
     1741 tctgaaaaat gagatttgtt ttttgaataa cttgtagctt agataggtaa atggcaaagc
     1801 aattgctaat tgtggaatgg cagcatgact atagctgcga tgtacaagta atgcttcaga
     1861 ttcacttaaa tgcgtaaaga aatgcaaaaa tacatctaag tcaggaatgg tgcctgctat
     1921 ggcgccaaag agcaaagcct tattgccaat tttttttcct aaaaccaact caccaacacc
     1981 agcgcctaag gcaatttgtg aaatcgaatc catctttact atctatttat gtaggcaggg
     2041 acaaagaaaa taccattaaa ataactcgcc ttgctctggt tcttttttat cgtttaaatt
     2101 tgtgatttcg aaatcaacat caaagttgac ggcttgttct tctgcttcgt ttgttgcaga
     2161 ttctattaat ctaatatctt tgatagtgcc accaatcagc ttgtttccaa tggctttcca
     2221 gccttttact tctataaatt ctagtaaatt aatcgtgcta ggctcgcttg tttttccttt
     2281 acctaaatcg acagtatatt caatcgttgg attgctgaac aatgttgcaa aaacaagctt
     2341 cgcattattc tcatctgata taaaattatt tttagtatcg atagatgttg tttctatttg
     2401 gaaacgctta acataatttg ctttcttact atcagaataa tgaacagcag aaatagttgt
     2461 tgttggatag agtttatcta ttgcgacaat atcgttcatc gtatatcgat ttgataaatc
     2521 aaaattagtc agataatatt cacctgattt aagtactact aaaattctgt catcattttg
     2581 gaatgagcct aaatatctgc cacgtttatc aatatttaag cgacctaccg tatcgtctaa
     2641 ccaaatttcg acgccaccta aggttgttct tccttttcct ttttgttcaa tttttttcac
     2701 aggatatttc gtgacaatat ttccatttgc acttctacct ttgatgtcta aattataaaa
     2761 atcaaattca aaaactttat tgcgcgcact gcaatttgga gaaaggacaa cgctacagct
     2821 ttctgcttca ccatttggat tgaccgaaaa atatagaatt tttgatttag gattaccttg
     2881 cgttatcgga tattctttat cgcgtgtgat ggcagtaaca ttaaatcgtt tagcataact
     2941 ggttccagat ttaccatcta aataaactgc gttataagtg gtacgttcat catttttctt
     3001 ccaaactgca acatggataa tatcttttcc tacaaaaact ttttctgcat tgcgcacaac
     3061 tttcatcgta ccatctttca taaaggcaat aatatcatcg atgtcagagc attcagcaat
     3121 gaactcttct tttttcaaat ctttaccaaa accgataaaa ccttctgcac gattgacata
     3181 tagtttttcg ttattcgctg ctactgtttt tatttctact gtatcaaaga tggcaatttc
     3241 tgttttacgt tcttttcctt tgccatattt tttcaataaa tcttcaaaat acttaatcgc
     3301 atatttagtc aaagatttca gattcttctt agtctcttcc atctcatctt ccaaaccttt
     3361 gatatattcg tccgcttttt tcttatcaaa tttggagatg cgtttaattt ttatttccgt
     3421 caatcgagtg atatccgctt ctgttacctc acgcatcaat aattttttga aaggtgtcaa
     3481 tcctttatca attgcggcaa taacaccttc ccaagtttct tcttcttcaa tatcacgata
     3541 gattttattt tcgataaata ttttttctaa tgaagaaaaa tgccatttat cttccaactc
     3601 gttcaattga atttccaact ctcgttttag caggtgaacc gttgtatctg tcgataattt
     3661 tagtgcatca gttgcgccaa taaaatgagg tttatcatcg ataataacgc aagtatttgg
     3721 agaaatggat aattcacaat ctgaaaatgc aaacagcgca tctacggtaa cacttggcga
     3781 aacttgtggt tgtaattcga ttaatatttc cacttctttg gctgtgttat caactacttt
     3841 tttaattttt attttacctt tttcattggc tttcaaaata gaatcaatta aatccgaagt
     3901 tgtagtacca aatggtattt ctgtaatgcg taatgttttt ttatcgacga tttccatttt
     3961 cgctcgcacg cgaattctgc caccacgttt gccgtcatta tattcgcgca catctactct
     4021 accgccagtt aagaaatcgg gaaataattc aaaaggttct tttcgcaaat attttatcga
     4081 agcatctatt aattcgcaaa aattatgcgg catcactttt gtcgataaac ctacggcaat
     4141 tccttctgca ccctgcgcta atagcatcgg aaattttaca ggcaattgta ctggttctct
     4201 ttttcttcca tcatacgaga cttgccaatc tgtagtatca tcgttaaaca cgacatctaa
     4261 ggcaaactta gacaaacgag cttcgatata acgagctgca gcagcactat cgccagttct
     4321 gatgtcgccc caatttccct gacaatcgat taataaatct ttttgaccta tattgacaat
     4381 tgcatcatta atacttgcgt cgccatgtgg atggtattgc atggtgctac ctacaatatt
     4441 ggctacttta ttgtatctgc catcgtccat ttctttcatg gcatgtagaa tacgtcgttg
     4501 aactggcttg aaaccatcat acaatgctgg cacagcacgt tctaatatca catacgaagc
     4561 atagtctaaa aaccagtttt catacaagcc gtctatggta gtaatatggt cgctatgttg
     4621 ttcgttaaat tcttctgttg acatattatc ttattgtatt acaatatttt tttagttttc
     4681 tactaattct gctgctactt cgtctttttc gactttcaaa ttattaatga taaataattg
     4741 tctgtctggt gtattttttc ccatataata ttcgagcagt tgctctatac tttgattgct
     4801 ttttaaaaca acaggttcta atcgaatatt atcgccaata aactgttcga actcatttgg
     4861 cgagatttcg cctaagcctt taaatcgtgt aatttcaggc ttgccacgta gtttatcgat
     4921 agcctgtatt ttttcttgtt cagaatagca ataaatcgtt tcttttttat cgcgcacgcg
     4981 aaacaaaggc gtattcaaca catataaatg tccttttttc actaaatcag gaaaaaattg
     5041 caagaaaaaa gtcattaata ataagcgaat atgcatacca tcaacatcgg catcagtggc
     5101 aataacaaca ttattatagc gcaaattatc gacatcttct tcgatatcta atgcatgttg
     5161 caatagatta aattcttcat tttggtaaac tatttttttc gttaaaccat aacaatttaa
     5221 aggctttcca cgtaaggaga aaactgcttg tgtttgtgga ttgcgtgctt tggtaataga
     5281 gcctgatgca gaatcacctt cagtgataaa caacatcgta tctgcatgaa gttcattttt
     5341 ttcgtcagta taatgtacac gacaatcacg caatttttta ttatgtagat ttgctgactt
     5401 cgctctttca ttggccagtt ttcgaatacc agacaaatct ttacgttcgc gttcagactg
     5461 ctgaattctt ttcaacaatg cgtcggcaat tgttggattt ttatgtaggt atgaatctaa
     5521 tttatctttg ataaaatcat tgacaaatga ctttaaactt ggcccatttt cccacatatt
     5581 taaggagcct aatttcgttt tggtttgcga ttcaaaaact ggttcttgca cacgcacggc
     5641 aatggcgcca acgatagctt gtcgtatatc agatgcttcg aattgttttt tataaaactc
     5701 tcgtaaggtt ttgactatcg cttctttaaa tgcagccaaa tgcgttccac cttgtgtcgt
     5761 atattgacca ttgacaaatg aatagtattc ttcgccatat tggttattat gtgttaatgc
     5821 aatttcgata tcattatctt ttaaatgaat aattggatag cgcaattcag cttcatttgt
     5881 tttcttgttc aataaatctt ttaaaccttc tttagaaaca aacttttggc cattgaaatt
     5941 aatagttaat ccagcattta gataggcata attccataat tgctcttcga taaactctgt
     6001 gacaaaatga aaatttttaa aaatagtatt gtccgcatga aattgaaatt ttgtaccatt
     6061 tctatctgtt gtttttgcaa ttttatggtc ttgcattaac acgcctttat caaattctgc
     6121 gactttaata tcgccatcgc gaacagactc aactttaaaa taagatgaca atgcattcac
     6181 tgcttttgta ccgacgccat tcaagcctac tgatttttga aaagcacgtg aatcatattt
     6241 tccgcctgta ttgattttgg agacacaatc aataacagca cctaatggaa taccgcgacc
     6301 ataatcacgc acagtcacag ttttatcttt gatagataca tctatttgtt tgccatgtcc
     6361 catgacaaac tcgtcgatag agttatcgat aatctctttt agcaacacat aaattccatc
     6421 atcaggtgct gagccgtcac ctaattttcc gatatacata cctggacgca aacgaatatg
     6481 ttctttccag tcgagcgagc gaatgttatc ttcggtatat tgaacttctg ccatttaatt
     6541 aaattaatac atttgaataa agcgatattg tcaaaaaata acttgacttt caagcaacaa
     6601 aagtatagat atagtaatgg aaattttatt aaatttttcc acaaggttgc atattaaata
     6661 agtagctgat gcttatttga ttgtatataa ataagctatt gaaaggttga agttacaatt
     6721 atatgttttg ggcaaaccac ttttcaattt caccaaaggc atcataccaa tgtcttgccg
     6781 agcggctact tcaatccagc cttttcctac tttgatattt gtgcctgcgc caataatcat
     6841 tccaacatcg aatcgattat ctgttggact gaataagtat tttgaagtaa tagaattttt
     6901 agattgttta tcaacggaaa ctgattcttg cagatagcca cctgtttgat aggcaagata
     6961 tggccctaaa aagaagacgg cttttacttt ttggctacca actcgagcga ctgccaatat
     7021 tggaaattgc acgtaattaa gtactttttt tgcgctaaac tttgctgaat catttgaatt
     7081 tttccatcct tgaccttttt ggataaattc taattctgtt tgtaaaccaa taaatttatt
     7141 aaaatcgata cgtagtacgg ccgaaatatc agaacctaat atagctcttt tagaataagt
     7201 atatggagat ggatatttgc ggataaatgc aacatcaaag cctgcttttg caccgacaga
     7261 cacacctact tcagcgtatg cagacactag catgaagatg tagaatgata ttatagaaaa
     7321 cagccttctc attattttat atttcacaca aatatacctt agtttttcta actatcattc
     7381 aaatttgtta aactatactt aaaattttta ggaaacaaag cgcattatta taattggtat
     7441 tacatttgtt tctgttatca aaaaaagaca aacatgaaaa aattatttct agcactaact
     7501 atcacagcaa gcacatttgg tgcatttgcg caaactacac cagcagtagc acctgctgta
     7561 gatccaaatg ctccgacaat ggaatttaca aaagatgcta tcgactatgg cactattaag
     7621 caagatgcag atggcaacag aacgttttcg tttacaaaca atggtactac gccgatcatc
     7681 atcagcgaag cacacggctc atgcggttgt actgtaccaa cgtatcctaa agagccaatt
     7741 atgcctggtc aaaaaggaga aattaaagta cattacgaca cacacagagt tggacctttt
     7801 tctaagagtg taactgtaaa ctctaatgca aaaaactcgc cagtagtatt aaaaattagt
     7861 ggtacagtag aagcagcagc agtagttcct acagcaacag agccaagcat catacctgcg
     7921 ccagcaccag ctacaactac tccagcagta aaagcaacaa cagcgccagt taaaaaaaca
     7981 acagcgccag taaagaaaac aacagctccg gtaaagaaaa caacttctac tactaaggca
     8041 aaaacaacaa caaaataatt gtttgttatt tagtaataaa aaaatcctga cttcgtcagg
     8101 atttttttat tttatctagt aagtattaca tattcactga taatttctcg taagaaatct
     8161 gcatcacttt gattgctgtc ttgtacaagg atatccattt gttgttttaa ttgctcaact
     8221 gtgtcgtgta aaagtatttg ataaacatca gtattaaatt gctgtgttct aattaataat
     8281 gttttcaagg cgagcatttc tttgtctgta taatgtacta cttgtggata ttttggaata
     8341 tattcatctt tatcaggcaa atttaatagg tcggtaagtg taacaactct atctggtttt
     8401 aaacgcacaa ctgtaacatt agtgaccatg tcgcccagtc gttgattttt ttcactcatc
     8461 aaaatcaaca aagaacctac tgaaaagaac gaaaaataga tatcgaaata attgtacatg
     8521 catcgtacaa aatacttata gaaatccatc gaattaccat ctaaacttat aatctttatg
     8581 ccgaccacct tcttgccaat cgattgacca tttaggcgtt tttcaataaa taattgaaca
     8641 agaataaata aagaaaaata cagtataaaa tatgccaata actcaaaaaa actaaaagac
     8701 aaacccaata aggagctcaa ccagttaggc ataaaattca atgcaagaaa tatgacggtg
     8761 ttaaagatga aaaaatcgat actccacgct attgcgcgcc acaaaaatcc agcagtttca
     8821 tattggataa taacatgctg tgtggtaggt atttcaattt tagccataaa ctaaatatag
     8881 gaagaagaat cataatttca aatttgaaaa atcttgaaaa acaaaatagt aaacatcatt
     8941 agtaaaatga aagatgatta caattaaatt ttattatttt agtaattcat gaaagagagc
     9001 aaattcatag aacaaaacca aaaaaaatgg gctgaattcg aagaaatgca aaataacaag
     9061 cataaagaca acaatcatta tagcaagtta tttatacaaa ttactgatga tttatcatat
     9121 gctagaacgt tttatagcaa tcgttctgtg cgtttatatt taaataaatt agcacaagat
     9181 atttttcata aattatttag taaaaacaag aaacaaaaat cttttttcaa agatttttgg
     9241 ttggaagaat tgccgtctgt tatgtattat cacagaacag aattactcgt aagttttacc
     9301 acacttataa tcgcaatcgg cattggattg cttacgtata tgcacgacaa tgaatttgcg
     9361 gcaaaaatac tgggtgacac ttatttgcaa atgaccgcag aaaatatagc aaaaatagat
     9421 ccgatggcgg tttacaaaag tatgtctcca gtgcaaatgt ttaatcacat tgtaattaac
     9481 aatctacgtg tagatatcat cacttttttc tttggtatta tattttctat aggatcgata
     9541 tttgtgttga tacacaatgg cctcatgctt tctgcgtttc aattttattt tataaagcaa
     9601 ggattatttt gggaatcatt tttagctgtt tggctgcatg gtacattaga aatatctgct
     9661 atggtaattt gtggtgcggc tggcatcgta ttgggcaaag gacttgtgtt tcctggaact
     9721 tacagcagat cgcaagcttt tttttcatcg gcacaacgtg ctactaaaat atttattgct
     9781 gtgcttccca taacaattgt tgcagcaata gttgaagctt ttttgacgcg ttacacagaa
     9841 acaccagatg tcattcggtt tatttttatt ttattctctt taggttttat tctcgcttat
     9901 tttgtgtatc tgccttggct taaaaataga aatggaacat tgcgtgcgcc aacaaactct
     9961 ataatattag ccgaaaagca tgcggctttc gattattcaa gtataaaaaa tatttctaca
    10021 attacacttg aaacatttag tatgcttcga aaatatagtc atcaatttgc aagtataatt
    10081 ttttggagtt gtattattat gctctttatc ggtttttatt acaatcaaga atacatactt
    10141 gaaaatagtg aacaagattg gaaattcaat atgattttta cttttacaga caacattaga
    10201 ctgaacgctt tttgttatgt atatccaatt ttatttattg taattgcttt cctttgcaat
    10261 tacctttttt gtgtagaaat gcaaataaaa aataaacaag aaaaggaaac aatcatgcaa
    10321 tggttattaa aaaacaaagc agcatttatc atctgtgtca tctatcaatt agcctactat
    10381 tatattttat tcaatagaga aacaaataca agtacaatat tattcgcact gccttttcaa
    10441 tttatggtga gttatgtttt attttttccg cgactacaaa aagagaattt tgtatttact
    10501 acatttcgca ttactgttac tggtttatta aaaataatgg ttttgaattt tctatttatt
    10561 gcgtttatat gccttttata tgcgctatta aactcttccg tttattggat aatctatgaa
    10621 tttgtaaata caaacttaac agacaatgta tcaacacgag ataaggctta tctcctatta
    10681 gaaataatta ctgtacatag cattattgca atagcaagta ttattttctg tatgtatcaa
    10741 ggaatatttt atttttctca aaaagaaaaa attagtgcag aggttttgaa agcagaactc
    10801 gaaaatataa aaatcgctaa aaataaaaat gtattattta aataaaatat tcatcctgct
    10861 tattcttatc aaatgtaaca ctattgttgc acagcaaaat aataacattg ataatgaatc
    10921 aaacattgag aaacaagaca aatatttaca tcagcacatt gataaaaaag aatttgataa
    10981 gcataaatgg gaaacattaa gagaaaaaac aaaaattgat gaaagctttc aaaaaaggaa
    11041 tcaagaagct gctcaaaata ataaaccagc acaaaacaaa gctatttctg gtacattaga
    11101 aaatatcata aaatggactg tctacattat tatatttggt attctgatgt acttgatatt
    11161 tcgtgctatt aatttttcaa tatttaacaa aaacaaaaag ctcaaaacaa aattttctat
    11221 tgaagaaatt gagaacgact taaacaatgc ggaaatagat ccacatcttt atgccgcaat
    11281 aaaatctaaa aattacaaat tagcaattcg cttatattac cttaaaatca tacaaaagct
    11341 tcatgaaaaa gagaaaatca tttggagaaa acacaaaaca aacaaacatt acttacagga
    11401 aatgtatgac aaggaagact atataatttt taaagaaata actcagcaat atgaaaaata
    11461 ttggtttggc aacacaacaa ttgatgaaac gaaatacaac acgttaagta tggaatttgt
    11521 aaactatcat caaaatataa aataattgag caaggattct aaaaaatata ttttagtatt
    11581 tatggcagtc attattgtca taattgctta ttttttatta cgtgattata atgaaaaatg
    11641 gaatttaaat tttgataaaa caaaaaaaaa tgcatacggc acttacatta cgtatgagat
    11701 tttaaaacac aataatgaca gcaacttcat tgaaataaac aatagtgtta tcagcacttt
    11761 taggacatta gataaaaata aaaaatacaa ctatgtattc attaattacg ccaattttta
    11821 tgatagcgca acaatagata ccatttgtaa atttgtcgaa aatgggaata ctgctttctt
    11881 ggcagttgag gacatatact attccagaat attaagcgac tctatacttg aacagcaata
    11941 tcatttgcgt acaaatacag atttgttttc tgaatcagat acaattgtaa aagataaaaa
    12001 tagtgcaagg ttcaatttta tcaatccatc attacaaaat aaagatggat attgttatta
    12061 tgttcgcgat gagtatgaca ctatacaata cgcattcaac cattttgaag caattcctga
    12121 cagcgaatgg actgcaacaa acaacaaaga aaaagaatta ttattatttg gcaataaaac
    12181 ggtaaatcat attaccaata attttatttc attgaagcat ggtaaaggaa acttcattat
    12241 attactatct gcaataccat tcaccaatta ttttatgcgc aaacaagaag gcttagaata
    12301 tgcagaaaaa gtatttgcac atttaccaaa caatacaata atttgggata atgtatcgca
    12361 atatgagaaa gaagaaataa atgatgacag aattaatgat agtagatata acgaaagtgc
    12421 tttatatttt attttaaaca atcgctcctt gcggtgggct tggtacttat gcttgttagg
    12481 tgtgcttatt tatgttattt ttcaggcaaa gcgaagacaa aacatcattc cgcttatcga
    12541 aacaaaacaa aatacatcac tacaatatgt acaaactatt ggccaattat attatcaaga
    12601 taaagcacat caagaaattg ccaaagagat gaagttgcaa tttttaaatt ttttaagaaa
    12661 taaatacaat tgcaaaacca atgaaattga caaggtattt atgcagcaaa tagccataaa
    12721 gtcaaacatt gacaaagaaa agatagcatc tatattcaat catttcaaca atattgaaac
    12781 agtgaataca actcaactac atcaactcaa cacagcatta gaatattttt ataaaaattg
    12841 taaataattt tatggaagaa aataaaccaa caaacgcagt agcatcagaa gacatcaatt
    12901 ttgtattgtc tataactgaa aaaataaaaa atgaagtcgg taaaattatc attggacaga
    12961 acagcaccat agaactccta ttagctgctt tatatgtcaa tggtcatatc ttattagaag
    13021 gtgtgcctgg tattgctaaa actttaactg caaaaatgct ggcaaagacg attgctgttg
    13081 acttttcgcg cattcagttt acaccagatt taatgccgac agatattgta ggaaccagcg
    13141 tttttaatat gaaaaatact gagtttactt ttaaacaagg tcctgttttt tctaacatca
    13201 ttttgataga tgaaatcaat cgttcgccag caaaaacaca agctgcgctt tttgaattaa
    13261 tggaagaacg tcaagtaact gttgatggaa ccacctatcc gatggaatat ccgtttttag
    13321 ttttagcaac acaaaatcca attgaacaag aaggcactta tcgtttaccg gaagcacaac
    13381 tcgatagatt catttttaaa atagaaatga aatatccaaa tttaaatgac gaaattgcca
    13441 ttttacaacg atttaattac gatttttcac aaacaataac gcaagatgta agcgctgttg
    13501 cgagcgcaaa tgacatcaaa aaatgcagca atattataga aaaaatattc atcaaagatg
    13561 aactcatcaa ctacattgcc aatatcgtat atgaaacacg caatcatggt gatttatttt
    13621 taggagcatc gccgcgcgcc tcactttcat tattgaaagc aagtaaagca atggctgcta
    13681 ttgctggcag agattttgtt attcctgatg acattcgcgc agtagctgac gcagttttaa
    13741 atcaccgcgt gataccttcg gcagaaaaag aaatggaagg cattacagca aaagacatca
    13801 tagctgatat tataaataaa attgaagtgc ctcgctaatt ttactttata gaatcttatg
    13861 caaatcttaa aacaactata caaaactatt tatttacaca cgtttttgac gaaacgattt
    13921 tttataacat ggttagtcat tattgcagta tttactatta gtttcaatta tagtttttta
    13981 tttacaatcg gaaaaatact gttgattttg ctcacagtaa tcagcgtctt agattttata
    14041 tttgttctta tttatcgacg aaaaattgaa tgcgtacgaa ccgtgccaaa tatcttgtct
    14101 ttaggtgatg agcaaaatat tacattacgt ataaaaaata aaaatctatc tccaataaaa
    14161 attgaagtaa tagacgaatt gcctatcgaa tttcaaaaac gagatttttc tttcaaacta
    14221 aatatgagtg gcaatgaaac aaaaaatata ctctacaaca tcagaccgct aacacgtggt
    14281 gcatatacgt ttgggaattg taatattttt gtgtccacat tattaggttt aatacaacac
    14341 aaacagattt ttgccttgca aacaggtgta cctgtttatc catcaataat acaactcaaa
    14401 aagcatgaac ttcaactttt caataaattg cattcatttc aaggaataaa gaaatcaagg
    14461 aaaattggac acagctatga attcgaacaa ataaaaaatt atgcaagtgg tgatgattat
    14521 cgcacgataa actggaaagc aaccagcaaa aaaaatcagc tgatggttaa ccagtttggc
    14581 gatgaaaaat cacaacaagt atatagcatt atcgataaaa gcagagccat gataatgcct
    14641 tttaatggtt taagtttatt agactatgcc atcaacagca ctttagttat ttccaatact
    14701 gtcttgcaca aagatgacaa agcaggccta ctcaccttta atgaaaaaat gggaaatttt
    14761 gtgaaagccg ataggaaaat aggtcaacta aaacgcatta cagaagtatt atacaaagaa
    14821 gaagaaagca gctatgaagc caattatgaa ttgctatacg acgccgtaac taagctcata
    14881 aaacaacgaa gcttgcttat tttttacacc aacttcgaaa gttattacac actggaaaga
    14941 tacttgccag tttttcgtaa aataaacaaa acgcacttat tgctccttgt cgtatttaaa
    15001 aatacggaaa tcgaaaaata tagctacgaa acagcaacag acacgctgtc tatttatcaa
    15061 aaaacaattg gcaagaaaat gttggcagaa aaagaaatga tgctgaaaga attagcaaaa
    15121 tatggcattc aagcgctgct cactgcgcct caagatttat ctacacaaac catcaataaa
    15181 tacttagaat ttaaggcgaa aggctggatt taataagcta ataatattta ttgcgttaca
    15241 taaactattt taacgaataa aatattgaat tttaatggta gaattcagat atttgaacaa
    15301 ttgaaacgtc taaatttttt ctactctact tgatcattag caagcattca tgaacaccac
    15361 aagaaattgg atattactca cattatttac attgctgtca ttttttacag tcagatggtt
    15421 agccaattat caagagaaaa aaaacaccat taccgaacct actgttgatg tcgatattga
    15481 tgccatcaaa gaacgtggca ctttacgtgt gattatggaa tataattcta tcagctattt
    15541 tatatataaa ggtcaacgtt taggttttga atttgaattg atgcagcatt ttgcaaaagc
    15601 aattggcgta aaactagagg tcataatcgc taaaacaaca gacgagcaat ttaatttatt
    15661 aaatgaagga aaaggcgata ttattgccaa tggcattata caaaacagca agataaaaca
    15721 acaagtagca cttacacaag cctacagaga tgtagagcaa gtattggtgc aacgacgttc
    15781 tggcattagt gttaatccaa ttgacagcat tcaaaaaaat gatacgcttt tacaaaacgt
    15841 aaatgaactt tctaataaaa ttgtatatgt tgcagacaac tcttcgtatt atactagttt
    15901 gaaagaatta tcggattcca gtggcatcaa catggacgtt cgtattttac cagatgagcg
    15961 tggcgttgat gatattattg aagccattgc aaatggagaa atcgattaca ccattgccaa
    16021 taaagacata gcattgctca acaaatctta tttcgaaaat cttaatgtcg atgtagctgt
    16081 tggcaaaaag caagccttgc attgggcagt gcgcaagaac gctcctgact tgctgagcag
    16141 catcaactat tggcttacta aatttgaaaa aggaaaaaca ttcagcgcac tttattataa
    16201 atattttaat gaagaaaaaa atgtagctta tggttataat gaaaatgcag aattgcaaaa
    16261 aggtatgatt tcacattacg atcagctaat acaatgctat gccaaaaaaa taaattggga
    16321 ttggcgtttg gtggccgcta tcatttatca agaatctaaa tttaatccga atgcacgctc
    16381 atggtgcggt gcacaaggct tgatgcagtt gatgccaggc accgcacgac aagttggtgt
    16441 gtatagtagt gaagtgtaca atccggaaag taatgtgcga ggtggaactg aatatttacg
    16501 acaattagaa aaaacatggt cgcatattac agactataca caacgtatca aatttatttt
    16561 agcatcgtat aatgcaggac ctggtcacgt attagatgct gcgcgacttg cagaaaaaaa
    16621 tggttatcca aaagataaat gggatggctc tgtagaatat tttattttgt ataaatcgaa
    16681 tccaagattt tacaatgatc ctgtggtaaa atatggttat tgtcgtggac aagagccgtt
    16741 taactacgta agaaatatta tcaaaaaatt cttcgattac gaaacaaaaa taaatacagc
    16801 cacagcgtct gcaacgccaa tggatatgcg ccttgagcaa gtagaatcaa ttccatttga
    16861 tggcattgaa ggtgcatata atccaacgca aggcttgata gcgcgttctg ctcgtcgcga
    16921 attatttcta tcacgaaaat tatttggtga agaaacacaa ttgataccaa aaaattcaaa
    16981 cagaaatcct ttcgacaaac ctaaaaaaga gctgttcatt cgcgaaaatc caaaagaatt
    17041 tgccgattca tcaaggcaat tattcaagaa gaaaaaacaa ttatttcaag tgcaatcgcc
    17101 aagcaattct tccaacttgc agctcaacga aaatgaagaa aaaataaatc aactgacgcc
    17161 aagaagataa acaaacaatt ataagcgtac aatgagttga gcaccgctgt ccgtcagcgt
    17221 cggtctttgc atcttagctt gtaatgaagc ataaaattca gtcgattgct gttgtagtaa
    17281 aagctgtaat gcttgactgc aattttcttt cgtacaatca tcttgaatca attctttcac
    17341 caacaaattg tcaacaatta aattaggcaa agaaataaaa cgaaccttag ccaaacgttt
    17401 acctatttga taatttatcc aagaggtttt ataaactacc acttgaggca ctgaaaacaa
    17461 agccgtttct aatgttgctg tgccactaca cacaatcgct gcctttgcat attgcaatac
    17521 atcatacgtt gcatcatata caatgcttac attattgggc aatggaattt gatataactc
    17581 atttactgct gacatgcctg caatgacaaa tttttcatta ggaaaatcgt tggttgtggc
    17641 aagcatcatt ggtagtaatg ctttaatttc ttgccggcga ctgcctggca ataatgcaat
    17701 atgatttttt gctgtagagc ttgattcatt cactatttta gcaatactat cttgcaatgg
    17761 atggccaaca aaatatgctg tcgtgtattg atgtttttga taaaatggaa tttcaaatgg
    17821 aaaaacacat aacatcacat caacatattt tttaattatt tcaacgcgac tttcattcca
    17881 agcccaaatt tttggagaaa tataataggc cacttttata tgatgttgct tacaccattt
    17941 tgcaatgcgc aaattaaaac ctgcgtaatc aactaagata acgcgatcag gttttgtttg
    18001 cagaatggaa ttttttactt ctttaaaatt gcgccagata gtaaatatat ttttgacaac
    18061 ttcaaaaaaa cccataaatg ccacacgctc taagccaaat atcagtttca cgtttgcagc
    18121 ttgcaaagca ggtccgccaa cgcctataaa tacagcatct ttatcaatag cttgtatatg
    18181 tttcactagt tgactgctat gcatatcacc agaaacttca gcggctatga taaaatattt
    18241 caagcttttt aaggaaaata ttacaataaa tatttaacag aaaacatggc aatgatatat
    18301 agcaatgatg ccacaaaaat gcccatgcat aaagaaaatt tctttaataa attgaacaag
    18361 aaaaagaaag gcagattgat aaacacacaa accttaataa acgaaggtaa aactgataag
    18421 tctttaacac ttgcaacata ttgatcccac ggcaagtagc taaatttaat ttctctgtaa
    18481 ataacaaaca taatcatcgg caataccatg cctaaaataa aaccatttaa tgttgtatct
    18541 agtttgtcaa gccatgttgt ttgagatgtc gaatttgatt gcatattaat ttttatgagt
    18601 ctaaaattaa atctttaaaa gtaaaaagga gaaaaaatta tttgcttaat ttaagattgt
    18661 ataattgacc aatgccagca gcaatgccac caataccact gataagatgt gtttgaaaat
    18721 caccaacttg aaaagctaat ttcaataaag tagcagcaca caacaaaacg agtccactga
    18781 aaaatataaa agcaggaatt ttgttgtgat gatgatgttt atagccatga agaatggacg
    18841 aggtgccgaa caaaaatacg ctcgcgataa ttacatattc tacccagtgg tattgttcaa
    18901 aataagctcc tgcaactggc aaaaccacaa ctaaaatggg tgtaatgata caatgaattg
    18961 tacacaaaac agatagaaaa attgtaaact gttctgaata atgtttatgt ttatgactat
    19021 ctttgactac gctcattttt gttattgtgt tgcaaatata caactttatg caactaaatt
    19081 gcaaagaaaa catactttat ttatgtctaa aatagaagag atactcaaaa accacgattt
    19141 acgtattacg caaattcgcg ttgatatatt gaattatttt ttaaaagaca agtcaaatgc
    19201 gcttacgcat gctaatttag agcaacaatt tggcaaaaaa cacgaccgtg taacaatata
    19261 tcgcacttta aattcctttt tagaaaatgg attagtacat aagattgctg acgactcagg
    19321 agtcgcaaaa tttgctgttt gtcaacatgg aagcagcaaa cat
//
LOCUS       DAOQQV010000041        19270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_861_length_19270_cov_6.499037, whole genome shotgun sequence.
ACCESSION   DAOQQV010000041 DAOQQV010000000
VERSION     DAOQQV010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19270
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_861_length_19270_cov_6.499037"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..1511)
                     /locus_tag="PLL59_05945"
     CDS             complement(435..1511)
                     /locus_tag="PLL59_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23382.1"
                     /translation="MKFKIFVTAITLSFFMSCQKDNDQTTDNYLEKGSAMIINEGGFT
                     KNNGSISFITRDNKVINNIFEQANGGRSVGDVLQSFTRIGNLGILCVNNSQKVVFVDV
                     PSFKQIAEITTGTDYPRFAIAINDEKAYISNGSFSGQVLVVNLKNFTVTKTINVGGGP
                     EEMLSSNGKVFVANSGGFGTDNTISVINTATDKEISRITVGDNPTEMERDAQGYLWVL
                     CKGNVTYSPPTYMPVRHSAAKLVRINPALDGIDKEIEIVASNNNFSTADNLAISGDGS
                     KIFVCVDDKVYQLPYSATSLPATPIITHLFYGLDVHPYTGEIWGLDAGNYNESGKIIR
                     YNSSAVAVDTFRVGIIPNAVYFNL"
     gene            complement(1508..3457)
                     /locus_tag="PLL59_05950"
     CDS             complement(1508..3457)
                     /locus_tag="PLL59_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMY23383.1"
                     /translation="MQKKIGYIIGVLFVLLTKCHYAADVLDSVQHIDTVRIHGNKPSA
                     NLFNSYSIIEKENSIASLNTINGIADVLQRFTSAQVNSYGAGNIASISIRGTNDDQSN
                     VYWNGIKLNSLTLGTTDLSIIPIESTDALLIDNNSSAIGGNVQLQTRPNWSNIVSIKA
                     KSDLSSFDNYKNGISLKVGNSRIQFHSDVYYHTAKNNFTFKDIYKFGNPIDTANHNQL
                     KSIAAINQLFFQFRKQIFISIGSLYNKKDKDLPAIMGTNELSSKFQKDKTLRHFITID
                     KSIKNGDFNFSFSHAFDELLYTDKRLPTDTFLFINSNYKSHRIASTFKSNSYFKYKLS
                     LQTGYTYCIQFAQVKEYGKKHVLEHIGDVYAKLKWENNNFNSSLSITQPFSSFKYIRP
                     QFTWSAAYKTNNTNKQYTIQLLYSDTYRFPDMNDRYWSPGGNPKLKPEHGWKMSVDNS
                     FRLLKNKNQFSVNTSVYVSIINNNIVWTPITNAIWTPKNLKKTRMYGVETAVDYSYRD
                     ADKFLFQLQAMYLFNKSQILQDYLQHQLEGKYLRYKPQHSIKLNMYVEQMFVGFGANY
                     TYTSLRYTDEDNLSYYMLKPFHLLDAFITFKGTIQDKHTLQFLFRINNITNTAYESIR
                     SYAQPLRNYTISFIYKFSKHQTTLK"
     regulatory      complement(3482..3675)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3741..4541)
                     /locus_tag="PLL59_05955"
     CDS             complement(3741..4541)
                     /locus_tag="PLL59_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HMY23384.1"
                     /translation="MISNIIKNKSTKLFIFLAGFFITNAFIAEVIGVKIFSLEDTMGI
                     NPFAIPLFGNTFSFNLTAGVLLWPVVFIMTDIINEYYGERGVKFLSYLTVCLLAYAFF
                     MYYFAIHLVPASWWNGSKVEQGVPNMQAAYAQIFGQGIAIIVGSLVAFLIGQIVDVVI
                     FHKIKQWSGEKYIWLRATGSTIVSQFIDSFVVLMIAFYWYPKYISEANGKAWPFALVM
                     SICIGNYIYKFIIAVVLTPVIYIVHYFIEIYLGKETATAMKNAALKAK"
     gene            complement(4594..6216)
                     /locus_tag="PLL59_05960"
     CDS             complement(4594..6216)
                     /locus_tag="PLL59_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMY23385.1"
                     /translation="MNNYVKYIIIVAFANILLTRCDSATSSKNDVFHMNISAGLTSLD
                     PAFSKDQATMWCDNQLYNGLVQIDEQLNVQPCIAKYWKISTDGLLYEFILRNDVYFHD
                     HEKFENGKGRKVTAHDFVYSFNRLIDSTVASTGGWLFNDKIDKSNPFTAINDTTFQIH
                     LKTPFFPMLGMLTLQYCSVVPKEVADFYGKDFRKHPVGTGPFKFVRWEENNVLILTKN
                     QNYFEKDSTGQQLPYLQGVRISFISDKGIEFLQFSQKKLEFITGLDIAYKDKLLTREG
                     ELQAAWKDKIKFEKMPYLNTEYLGISMAKQPCKALKNKKVRQAINYAIDRKKMITYLR
                     NGIGTPAENGMIPKGIPCYNANIVKGYTYNIDKAKQLLAEAGYPNGKGIGEFKIYSNP
                     TYSDLITYIANELANIGISCKIENTQATFLREAMRKNEVEFFRASWIGDYPDGENYLT
                     LFYGKNGAPPNYTFFKNEKFDKLYEAAMRETNQSTSCQLYHEMEQLIIEEAPVVPLFY
                     DEVTRFVHNHVVGLKPNAMNLVVLKEVKLKDK"
     gene            complement(6221..6745)
                     /locus_tag="PLL59_05965"
     CDS             complement(6221..6745)
                     /locus_tag="PLL59_05965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMY23386.1"
                     /translation="MITIFHGTARPNSNSRKIADFYSELLGSKNIAHQFFTLENVEAD
                     TFNKTFHNEKSAQQLMIEEQILAPTSKYIFIVPEYNGSFPGMFKGFIDSCNVKNCFHN
                     KKASLTGVAEGRAGNLRGIEHLTNIFNHMKMNVLHFKIPISQVHLMLDENGKLSNPEI
                     VRLIDMQIDLLLDF"
     gene            complement(6817..7809)
                     /locus_tag="PLL59_05970"
     CDS             complement(6817..7809)
                     /locus_tag="PLL59_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23387.1"
                     /translation="MKAEIKSIYDAHVAFELSQFDKKHLKANIKEEIAAAWKWLDTVK
                     LDDVASVDAVISFEERNIKERKLTKHQKEYIVELSESIHELALKSKHTVADFINEDSF
                     NDIAEKIISQKEMRERIIKDIVNNPFYGEMIADTLYDGIKSFAAQSGPSNETVGGSLF
                     NIGKGILGAALSGVSDTIDKNIKKFIADNLQKSIAKSEKTLKEKLNDAQLHKMAKNLW
                     EKLEETKVKDLAKGVKLTDKDRAAIVTSAEKAIHIAINSEPISELNEHVLTHFFEHSG
                     KKSIAQILEDNGITEKIVIKEADEFLIPYIEVALKDGFLKSRIEARLHKFYSTL"
     gene            complement(7996..8478)
                     /locus_tag="PLL59_05975"
     CDS             complement(7996..8478)
                     /locus_tag="PLL59_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23388.1"
                     /translation="MNTEYKQYPDESMVWVYQSNRFLKEEEKQHIENKVKSFIAQWES
                     HGSLVKGTYTLLHDAFVVIFADGEGQMLCGRAQSASVALMKELENELQIPFVDRMNLS
                     YKVGEQIHFINFNDLKNLYNNGVIQDDSLVFDNTIITKKDFENKWLCALENSWHKRYV
                     "
     gene            complement(8574..9416)
                     /locus_tag="PLL59_05980"
     CDS             complement(8574..9416)
                     /locus_tag="PLL59_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HMY23389.1"
                     /translation="MKTNFTKIAFYSFFVFALFTQCQQKSDSSAYSVNDAVAMQKEEI
                     ATDASAAPTNTNSTIPSQSADSSIQRMLTKEATLRWETSNIDKTHATIIEHSKKMNAY
                     IANDNQTRTEYEITNEMLLRIPSDKLDDFLTSIEKEVTRFDEKRIDVVDVTEEYIDVA
                     ARMKTKKELEQHYYDLLKQTKNVAEVLQVEQQLNNVRAEIESAEGRLKYLKDKVNMST
                     LRLTFYETTSAPVGFFGEIGKSFVAGWKGFLYFILGIIRAWPILLIITLISMYIIRKR
                     RKLK"
     gene            complement(9607..10317)
                     /locus_tag="PLL59_05985"
     CDS             complement(9607..10317)
                     /locus_tag="PLL59_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMY23390.1"
                     /translation="MGRAFEYRKAAKFARWDRMAKAFTRAGREIAMAIKSGGPSPDTN
                     ANLRRAIQNAKAAQMPKDRIEAAIKRASSTDTKGFSEVVYEAMGPHATAIVIETATDN
                     PTRTVANLRAILNKNGGSMGTQGMHDFIFERKGVFKISAANVDVEELEFELIDAGLDS
                     MEKTEDNHLILYTKFADFGSLQKALEDKNIEVVSAELQRIPTINKELNEEQTDEVLEL
                     IDKLEQDDDVQTVFHNLA"
     gene            complement(10507..11961)
                     /locus_tag="PLL59_05990"
     CDS             complement(10507..11961)
                     /locus_tag="PLL59_05990"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMY23391.1"
                     /translation="MEKNTFDVIIMGAGLSGIGTAYHLQENCKGKSYIILENRPSLGG
                     TWDLFRYPGIRSDSDMFTLGYSFKPWKYNEAIADGGLILNYIKETASENGIDKHIRFN
                     TKINSADWDSTTNRWTITCTDNKTNKTKTFTAKYVISCMGYYNYEKGHRPKFEGEKKF
                     KGDIIHPQFWPENYDYSGKDVVIIGSGATAVTLVPAMADKAKSVTMLQRSPTYIASIP
                     NRSLIPQQLLKNLPTKVQYGLNRAAQIGLQVTSYNLCQSQPELMKMYFRDHVKRQLPK
                     GYDIEKHFTPKYNPWDERLCAVPNGDLFKAIRSGKANVVTDHIEKFVEDGIMLESGKK
                     LKADIIVTATGLELQMLGGIKASIDGKEFKASDGIVYKGLMIKDIPNFAFVLGYTNSS
                     WTLKSDMVGEYFCRLINETERKGKNAFVVKSNKPIEREPALNFGAGYVQRAIQAGVLP
                     SQGVEYPWKLKQNYLLDAANFKFRSIHDKYLKFT"
     gene            complement(12120..12533)
                     /locus_tag="PLL59_05995"
     CDS             complement(12120..12533)
                     /locus_tag="PLL59_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HMY23392.1"
                     /translation="MSNINLQQYDKLVLFDGVCNFCEASVQFIIKHDKTNSLKFTSLQ
                     SEIGQQLLIHYKLSTAIEGVVFIENNIAYFKSKAAFKIANYFGGFWRVLQLFSILPTS
                     LTDFAYDIIAKNRYKWFGKKESCMLPSPDVRSRFL"
     gene            complement(12526..14442)
                     /locus_tag="PLL59_06000"
     CDS             complement(12526..14442)
                     /locus_tag="PLL59_06000"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23393.1"
                     /translation="MRIITTYLFLLITIQLMAQKESLTPLTYHTKMNKSTNQSFLQQK
                     VAHRISAISLPFFEDFYQQDIYPNPALWQDNFVFINTTFPKETVSVGVATFDGTNASG
                     VPYNTSGNTTHGPADMLTSNPIDLSGLSGADNVFLSFYFIQGDFGESPAAPNDLLTVQ
                     YKDTSGNWNIIWQVTALDSASLQQVFLKVDSIYLNANFQFRFQSFGNLNGANDTWHID
                     YVKLDKNRDTASEKNIKEMAYAFLPPSLLKQYYVMPYNQFDTTDLADTVSLFVKNNFI
                     NTTTDIVDFYEATDVSSSSTIATFSGPSRDFGPITLNKIDYPKFNIPTNLIGDTVVIN
                     VDYHFNVSAEAGEPAKVLANNAVSHQQVFSNYFAYDDGSAERGYLVGDLKTNTGMDFY
                     KMAVKYGLRNPDTLQAIKLQFFPVKPNNHLAVFSVCVWKNFTRNTVYNDNDLIYQETN
                     LKIEDLVNEYGVDTINGFYYAPIKPQFVKNGASYPLIMNDTFAVGLIVDNKLSLVVGF
                     DRNNIRSSYNYYVEGNTNKWRESGLPGTMMINPVMGKALPGYLTPVKETPAKNYTVKV
                     YPNPAKDKLFIEGVNDNTLLEIFDIKGSLVMQTTLQQSGYVDIHELPSSVYVIKITNT
                     HTNQIGTSKFIKNE"
     gene            complement(14453..15283)
                     /locus_tag="PLL59_06005"
     CDS             complement(14453..15283)
                     /locus_tag="PLL59_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HMY23394.1"
                     /translation="MGKTGLTILYIMGASVALLLFVYFGLNWYTDHGDFVVVPNVKGK
                     SLYDAKNELKNYDLDYIIADSTFDESKPPLSVLEQQPLNGAKVKENRRIYLTLNASAP
                     PSVKIPSIIDNSRRQAELILNSWGLKLGQLIYIPDMAKDAVLNIQVNGKIVKPGTVVP
                     KGTMVDLVLGDGFGNQVTEVPPITDMTVLEARAVLEAVHLNVGEIIAAGPIEDTLSAY
                     VYDQNPKFGQPGQLGANNSVQLYIRQQKSDIAEPEVQAAESNGMNKLVPKKNSNTTNP
                     "
     gene            15415..16500
                     /gene="ddlA"
                     /locus_tag="PLL59_06010"
     CDS             15415..16500
                     /gene="ddlA"
                     /locus_tag="PLL59_06010"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HMY23395.1"
                     /translation="MQKRKIGLVFGGKSPEHEISIISARNIYNAINKEKFEVILIGIA
                     QDGKWYLEDALNLQDEKCVIGKSGMQLAVVFGNEQHKLIRLDNQQYISDVEAVFPITH
                     GPNGEDGTLQGIFLQLDIPFVGPSVLGSAVAMDKDFTKRILRDAGIGHAKGITIYKHQ
                     IDNISYDAIVAELGNTLYIKPCNMGSSVGVSKATNEADFKKSIAEAFKFDHKVLVEKA
                     IIGRELECAVLGNEIIETSTVGEIVLEKGFYDFENKYKNDNAKIYIPAPNISAVQLEK
                     IQSTARDAYQALGLEGLSRVDVFLTDNDEVIINEPNTLPGFTSISMYPKLWESSGLKY
                     TDLIEKLIELSIERATREKQLQRVRLV"
     gene            16602..17018
                     /locus_tag="PLL59_06015"
     CDS             16602..17018
                     /locus_tag="PLL59_06015"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HMY23396.1"
                     /translation="MKKVILLMFLSIIVINTFAQKRNESKSPIIGSWKFTRQSTLNDF
                     QKVFKEKSYQAELFTFSGNQTFKHEFVDASEQVIKVLKGKWKIANDKIQIKYADVDYE
                     VYIDYFFLESDLVLGQNFSHVIFKKLDNEERNLTMK"
     gene            17178..17600
                     /locus_tag="PLL59_06020"
     CDS             17178..17600
                     /locus_tag="PLL59_06020"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HMY23397.1"
                     /translation="MKKAFFLMCLVAISVTALAQRKSETKNPLIGTWKFTNQTKVNEF
                     QKVFQNKNDFPIEYFTFNANQTFKHEFLDADEHTIKVLTGKWKKGSNSSITIYYKDIE
                     FELNTAYFFIDKDLVLGQNFSHVIFSKQDMFDKNIAMK"
     gene            18014..18409
                     /locus_tag="PLL59_06025"
     CDS             18014..18409
                     /locus_tag="PLL59_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23398.1"
                     /translation="MEFTQKFKEAVDSLTAETKANVTELRYNVENTVKNAVSEAKDEV
                     ELQRNHFKSTAERIQAKLKGPFTLDKVQADVKEEANILVADLKTTFNRNADRVKGLFN
                     EASAKVEKQVEEVKEEVKKATSKKTKATA"
     gene            18519..18767
                     /locus_tag="PLL59_06030"
     CDS             18519..18767
                     /locus_tag="PLL59_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23399.1"
                     /translation="MKIAGYIEHPIVKITVLHMNNRYVIKVEANMMEQTYKFHEDDKL
                     RTMADMEKLVDETFINECIKRFAEMNKSRGEMYQRNIL"
     gene            complement(18789..>19270)
                     /locus_tag="PLL59_06035"
     CDS             complement(18789..>19270)
                     /locus_tag="PLL59_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY23400.1"
                     /translation="SHVSTLYFSTEHAPISKPVVILNGNNGTTLVNHVFVPTLLHSNY
                     APEGKHLVAVNVVKDHDLDDEELVAKCLTELSEWFGLKVMDWQYLKTYHIKYAMPFKP
                     ILDEIKFVQAISDNVYACGDCFSVGSMESALRSGRETANVIAQEISLSKSMYDDAIA"
BASE COUNT         6350 a         3309 c         3006 g         6605 t
ORIGIN      
        1 gtagcattaa ctacgtcaaa agaatcgctt ggaaattgaa cattttcaac taaaggaata
       61 ttgactttgc ctttacgtgt atagataata cttatgttgc accacaaaag gtacaatgaa
      121 aaaatcagaa ataaagtaag tagtatatat ctgttcttca catactaagt ttaaacaaaa
      181 gtatgtaatt attatttaat gcgagaagaa atgtataaaa aagaaagctg actacaattg
      241 cttatccaca ttatattgat ttataaaatc taaaatgcag gattcgcata agcaatttga
      301 atattttgaa gttaaaatct cttgtacagg ctgaggtatg agcattttat tgcaaaaaca
      361 ttgtgaaata tacgctgcat tgcactcaaa tgttgagtga caatgcagac atattttctt
      421 caaagaggac aattttacaa attgaaataa acagcatttg gaataatacc aactctaaaa
      481 gtatcaactg caactgcact cgaattatat cgaataattt ttccagattc attataatta
      541 ccagcatcaa ggccccaaat ttcaccagta taaggatgaa cgtctaaacc ataaaataaa
      601 tgagtgataa taggtgttgc aggcaaagat gttgcgctat atggcaattg atacacttta
      661 tcatctacac aaacgaaaat tttagaaccg tcaccactga ttgctaaatt gtcagcagtg
      721 ctgaaattat tatttgatgc aacaatttct atttctttat cgataccatc taatgcagga
      781 tttatacgca ctaattttgc agctgaatgt cttacaggca tgtatgttgg aggcgaatac
      841 gttacatttc ctttgcaaag cacccataaa taaccttgtg catcgcgctc catttcagta
      901 ggattgtcgc cgactgtaat acgtgaaatt tctttatctg ttgctgtgtt gattacgcta
      961 atcgtattat cggtaccaaa tccaccgcta ttcgctacaa acacttttcc gtttgatgat
     1021 agcatttctt ctggaccacc gccaacattg atagttttag tcacagtaaa gttctttaaa
     1081 ttaacgacca atacttggcc agaaaaactt ccatttgaaa tgtatgcttt ttcatcattt
     1141 atagcaatag caaatcttgg atagtccgtg cctgttgtaa tttcagcgat ttgcttaaaa
     1201 gatggcacat ctacaaaaac aactttttgc gaattgttca cacacaaaat cccaagattt
     1261 ccaatgcgag taaatgactg caatacatct cctaccgagc gaccaccatt cgcttgttcg
     1321 aaaatattat tgattacctt attatctcga gtgataaatg aaatactgcc attgttttta
     1381 gtaaaaccac cttcattaat aatcatggca gagcctttct ctaagtaatt atctgtggtt
     1441 tggtcattgt ctttttggca agacataaaa aagctaagtg taatagctgt tacaaaaatt
     1501 ttgaatttca ttttaatgtt gtttgatgtt tagaaaattt ataaataaat gagattgtat
     1561 aatttctcaa aggttgtgca taagagcgta ttgattcata agcagtattg gtgatgttat
     1621 ttattctaaa aagaaattgc aatgtatgtt tatcttgaat tgttccttta aatgtgataa
     1681 atgcatctaa taaatgaaat ggtttgagca tgtaatatga caagttatct tcgtctgtat
     1741 aacgcaatga tgtataagta taattagcac caaagccaac aaacatttgt tcgacataca
     1801 tattcagttt aatgctgtgt tgtggtttgt atcgcaaata tttaccttcc agttgatgtt
     1861 gtaaataatc ttgtaaaatc tgtgatttat taaacagata catagcttgt aattgaaaca
     1921 aaaatttatc agcatcgcga tagctataat ctacagcagt ctcaacacca tacattcttg
     1981 ttttcttcag atttttaggc gtccaaatcg catttgtgat tggcgtccat acaatgttgt
     2041 tattaattat tgacacataa actgatgtgt ttacgctgaa ctgattttta ttttttaata
     2101 agcgaaagct attatctaca ctcattttcc aaccatgctc tggctttaat tttggatttc
     2161 ctccagggct ccaataccta tcgttcatat caggaaaacg ataggtatcg ctgtataata
     2221 attgaatggt atattgcttg ttagtattat tggttttata ggcagcactc caagtaaatt
     2281 gtggtcgaat atatttaaaa gaagaaaacg gctgtgttat agataagctc gaattaaaat
     2341 tattattttc ccatttcagt ttagcatata catctccaat atgctctaaa acgtgtttct
     2401 ttccatattc ttttacttgt gcaaattgaa tacaatatgt atatcctgtc tgtaaagaca
     2461 gcttgtattt aaaatagcta ttgcttttaa aggttgatgc aattctatga gatttataat
     2521 tggaattaat aaataaaaaa gtatctgttg gcagtcgctt atctgtataa agtaattcat
     2581 caaaagcatg tgaaaatgaa aaattaaaat cgccattttt aatactcttg tctattgtaa
     2641 taaaatgcct taatgttttg tctttttgaa atttgctact caattcattc gttcccatta
     2701 ttgctggcaa gtctttgtcc tttttgttat acaagcttcc aatgctgata aaaatttgtt
     2761 tacgaaattg aaaaaaaagt tgattaattg ctgcaataga ttttaattga ttgtgattcg
     2821 ctgtatcaat tggatttcca aatttgtaga tatctttaaa agtgaaattg ttttttgctg
     2881 tatggtaata aacatctgaa tgaaattgaa tacggctatt gcctactttc aatgagatac
     2941 catttttgta gttatcaaaa ctacttaagt cactcttcgc ttttatgctt acaatatttg
     3001 accaattcgg tcgagtttgc aattgcacat ttccaccaat tgcagatgaa ttattatcta
     3061 tcagcaatgc atctgtcgat tctattggaa tgatagataa atctgtagtt ccaagcgtta
     3121 atgaatttag ttttataccg ttccaataaa cattagattg gtcatcattt gtaccacgta
     3181 tagaaatact tgcaatatta ccagcaccat aagaatttac ttgagccgaa gtaaatcttt
     3241 gtaaaacatc tgcaattcca tttatcgtat ttagcgaagc aatggaattt tctttttcta
     3301 ttatcgaata ggaattaaac aaattagcag atggtttatt accatggatt ctgactgtgt
     3361 cgatgtgttg cacagagtct aaaacatcag cagcataatg gcactttgta agcagtacaa
     3421 ataaaacacc tatgatatat ccaattttct tttgcatact gaaatgtttc ctcagaacat
     3481 ttaatagtta atgaaaattg gcaggtcttc tgacttacac tttatcgagc cttcccattt
     3541 cgaagaaata gtggttgtag ttcgatacct tatttaataa ggtgtgctta cagcaactgg
     3601 gattgtacaa gacttacact tgtttccctt ttaattccgg atatgacacc gaaaaccaaa
     3661 ttactttgca atattacaac atatttgaga tatccaagaa caagtaatgt ttaaattatt
     3721 gttgactgat aaaaatcata ctatttggct ttcaaggcag catttttcat ggctgtcgca
     3781 gtttcttttc ccaaatagat ttcgataaaa taatgcacta tataaattac tggagtgagt
     3841 acaacagcga taataaattt ataaatataa ttgcctatac aaatcgacat cactaaggca
     3901 aatggccatg cttttccatt ggcttcgctg atatattttg gataccaata aaacgcaatc
     3961 attaacacaa caaaactatc gataaactgc gaaactatag tagaacctgt tgctctcagc
     4021 cagatgtatt tctcgccgct ccattgtttt attttatgaa agataacaac atcgacaatt
     4081 tgtccaatta aaaatgccac taatgaacca acgataatag caataccttg tccgaatatt
     4141 tgcgcatacg cggcttgcat atttggcaca ccttgttcaa cttttgagcc attccaccaa
     4201 cttgccggaa ctaaatgtat agcgaagtaa tacatgaaaa aagcatacgc taataaacaa
     4261 accgtcaaat aagataaaaa tttaacgcca cgttcgccgt aatattcatt tataatatct
     4321 gtcataataa acacgactgg ccataataaa acaccagcag ttagattaaa tgaaaatgta
     4381 tttccaaaaa gtggaatggc gaatggattt attcccattg tatcttctaa tgaaaatatt
     4441 ttaacgccaa ttacttccgc aataaaagca tttgtaataa aaaaaccagc caaaaaaatg
     4501 aacagctttg tcgatttatt tttaatgata ttcgaaatca tagaaatgca tttttggaaa
     4561 attatacaaa aaaaattata ctcaaaaaaa ttgctattta tctttcaatt ttacttcttt
     4621 taaaacaacc aagttcatcg cattgggttt taatccaaca acatgattgt gtacaaaacg
     4681 tgtgacttca tcataaaaca aaggaactac aggtgcttct tcaatgataa gctgttccat
     4741 ttcatgatat aattggcacg atgtactttg attagtttct cgcatggctg cttcatataa
     4801 cttatcaaat ttttcatttt taaagaaagt ataattaggt ggagcgccat ttttaccata
     4861 aaataaagtc agataatttt caccatcagg ataatcgcca atccaagatg ctctaaaaaa
     4921 ttccacttca tttttacgca tcgcttctcg gagaaaagtg gcttgtgtat tttcaatttt
     4981 acatgaaata ccaatatttg cgagctcatt ggcaatatac gttatcaaat cactatacgt
     5041 tggattggaa tatattttaa attcgccgat gccttttcca ttcggataac cggcttctgc
     5101 cagtaattgt tttgctttat ctatattgta tgtatagcct ttcacgatgt ttgcattata
     5161 acaaggaatg cctttgggaa tcatgccatt ttcagcagga gtgccgatgc catttcgcag
     5221 ataagttatc attttttttc tgtcgattgc ataattgatg gcctgtctta ctttcttatt
     5281 ttttaatgcc ttacaaggtt gtttcgccat tgaaattcct aaatattctg tattcaaata
     5341 gggcattttc tcgaacttaa ttttatcttt ccacgctgct tgcaattcgc cttcacgtgt
     5401 taatagttta tctttgtagg caatatctaa gcctgttata aactctaatt ttttttgtga
     5461 aaattgtaaa aattcaatgc ctttatcgga gataaaactt atgcgaacac cttgtaaata
     5521 cggcaattgc tggcctgtac tatccttttc aaaatagttt tgatttttag ttaaaatgag
     5581 cacattgttt tcttcccagc gtacaaactt aaatggtcct gttccgactg gatgcttgcg
     5641 aaaatctttg ccataaaaat cagctacttc tttaggtact acggagcagt attgtaaagt
     5701 tagcatgcct aacattggaa aaaatggtgt ttttaaatgt atctgaaacg tcgtatcatt
     5761 tattgctgtg aacggatttg atttgtctat tttatcatta aataaccatc caccagtcga
     5821 tgcaactgtt gaatcaatta gacgattaaa agaatacaca aaatcatgtg cagtgacttt
     5881 tcttcctttg ccattttcaa atttttcatg gtcatgaaaa tacacatcat tgcgtagtat
     5941 aaattcatat aaaagaccgt ctgtactaat tttccaatat ttagcaatac aaggctgcac
     6001 atttaactgt tcatcaattt gcactaaacc attatataat tgattgtcgc accacatggt
     6061 agcttgatct ttagaaaaag ctggatctaa tgatgttaat ccagcagaaa tattcatgtg
     6121 aaatacatca ttttttgagc ttgtcgctga atcgcaacga gtcaataaaa tattggcaaa
     6181 cgcaacaata attatatact taacgtagtt gttcatctat ttagaaatct aacaataaat
     6241 ctatttgcat atcgatgagt cgtactattt ctgggttgct aagtttgcca ttttcgtcta
     6301 acatcaagtg tacttgtgag attggaattt taaaatgcag tacattcatc ttcatgtgat
     6361 tgaaaatatt tgtaaggtgc tcgatgccac gcaagttgcc ggctctacct tctgcaacac
     6421 ctgtcaagct tgctttttta ttgtggaagc aatttttcac attgcacgag tctataaaac
     6481 ctttaaacat accaggaaaa cttccgttat attctggcac aataaaaata tattttgatg
     6541 taggtgcaag tatttgctct tcaatcatta attgctgtgc agatttttca ttgtgaaatg
     6601 ttttattaaa cgtatctgct tctacatttt ctaatgtaaa aaattgatgt gctatgtttt
     6661 tgctgcctaa tagttcgctg taaaaatcgg caattttacg tgaattacta tttggacgtg
     6721 ctgtaccatg gaaaattgta atcatttgat tttgaaattt ccataaaaat agtagctata
     6781 aaatctatat cacatgattt ttatccacaa acaagcttac aacgtagaat aaaacttatg
     6841 taaacgagct tcgattctgg attttaagaa accgtctttc agtgctactt cgatatatgg
     6901 tattagaaat tcatcagctt ctttgatgac aattttttct gtaatgccat tatcttctaa
     6961 gatttgtgcg atagatttct tacctgaatg ttcaaagaag tgcgtcaata catgttcatt
     7021 taattctgat atcggctcgc tgttaattgc aatatgaata gccttttccg cagaagtaac
     7081 aattgctgct ctgtctttat ctgtcaactt cacgcctttt gccaagtctt ttacctttgt
     7141 ttcttcgagt ttttcccaga gattttttgc cattttgtgc aattgcgcat cgtttaattt
     7201 ttcttttagc gttttctcac ttttagcaat cgatttttgc aaattatcag caataaattt
     7261 tttgatattt ttatctatag tatcagatac gccagacaat gcagcaccta aaatgccttt
     7321 gcctatattg aacaaagagc cgcccactgt ttcattactt gggccacttt gtgctgcgaa
     7381 cgatttaatg ccgtcatata aagtgtctgc aatcatttca ccataaaaag gattgttcac
     7441 tatgtctttg ataatgcgct cgcgcatttc tttttgtgaa ataatttttt cggcgatgtc
     7501 attaaatgaa tcttcattta taaaatcagc aacagtatgt ttacttttta atgctaattc
     7561 atgaatagac tcagacagtt caacgatata ttctttttga tgtttcgtaa gtttacgctc
     7621 tttaatattg cgttcttcaa atgaaattac agcatcaaca gaggctacat catcaagttt
     7681 tacggtatct aaccatttcc atgcagcagc aatttcttct ttaatatttg ccttaagatg
     7741 ttttttgtca aattgtgata attcaaatgc cacatgtgca tcgtagatgc tttttatttc
     7801 tgctttcata ctttcattta tttaaacgaa aataaaatta ttttcttagc agacaattaa
     7861 cctaggaaag attacatata atttaatgca gatttgagta caaatcgaaa tataaacctg
     7921 ctgattttga caaaaacata tttcaaattg ctgattttaa aatgagccag caattgtatt
     7981 tttcaattat tgcgcttaca catatctttt gtgccaacta ttttcaagcg cacaaagcca
     8041 tttattttca aaatccttct tagtgataat ggtattatca aacaccaaag aatcatcttg
     8101 aataacacca ttgttataaa gatttttcaa atcgttgaag ttgatgaaat gtatctgctc
     8161 acctacttta taacttagat tcattctgtc aacaaatgga atttgtaatt cattttctaa
     8221 ttctttcatc aaggccacag acgctgattg tgctctgccg caaagcattt gaccttcgcc
     8281 atcagcaaag ataacaacaa aggcgtcgtg taataatgtg taggttcctt tcaccaagct
     8341 gccgtgcgat tcccattgcg caataaaaga ttttacttta ttctcaatat gttgtttttc
     8401 ttcttctttt aaaaatctat tggattgata aacccaaacc atcgattcgt ctggatattg
     8461 tttatattcc gtattcattt gacaatattt tgaaatacaa aattaagcat ttctttaata
     8521 agaaattatt gatttgcgtc atttatgcag tcaaaaaaaa ggatagtata atatcatttt
     8581 agctttcttc ttttgcgaat tatatacatt gaaatgagtg taataatcaa taaaattggc
     8641 catgcacgaa taatgcctaa tataaaatac aagaagcctt tccaacctgc gacgaagctt
     8701 tttcctattt cgccaaagaa accaaccggt gctgatgtgg tttcataaaa agtaaggcgc
     8761 aaggtgctca tatttacttt atcttttaga tatttcagtc tgccttctgc actttcaatt
     8821 tctgcacgta cattatttaa ttgctgttca acttgtaata cttctgcaac atttttggtt
     8881 tgttttaata agtcataata atgctgttcc agttctttct ttgttttcat acgtgcagca
     8941 acatcaatat attcttctgt tacatcaaca acatctattc ttttttcatc aaatcttgtc
     9001 acctcttttt ctatgcttgt taaaaaatca tcaagcttat ctgatggaat tcgcaacaac
     9061 atttcattgg taatttcgta ctccgtacgc gtttgattgt cattagcgat ataagcattc
     9121 attttttttg aatgctcaat aattgtagca tgtgttttat cgatattgga agtttcccag
     9181 cgcaaggttg cttctttagt aagcatgcgt tgaatagaag aatcagcgct ttggcttggt
     9241 attgtcgagt ttgtattagt tggtgctgcg cttgcgtctg ttgctatttc ctctttctgc
     9301 atagctacag catcatttac tgaataggca gaactatctg atttttgttg gcattgtgtg
     9361 aataatgcaa agacaaagaa gctataaaaa gctattttag tgaagttggt tttcataata
     9421 tggattggtt tgcttgaaag gaaaacgtat tatcttacag catattgaat acttgaaaaa
     9481 tattaatata aattaacgaa aaaaaatcgt catcttaaaa tatactagag taaaacatat
     9541 taaagattgg caaataaaac tgaaataaaa ttcgtgctgt caagaaaata taacggaaag
     9601 tataaactac gctaaattat gaaagacagt ttgcacgtca tcgtcttgct ccaatttatc
     9661 aattagctct agcacttcat ctgtttgttc ttcgttgagt tctttattaa tggttggaat
     9721 acgttgaagt tcagcgctca ctacttcaat atttttgtct tctaaggctt tttgcagaga
     9781 gccgaaatca gcaaattttg tatataaaat aaggtgatta tcttctgttt tttccatgct
     9841 atccaatcca gcatcaatca gttcaaattc taattcttct acatcgacat tagctgcaga
     9901 aattttaaaa acacctttac gttcaaagat aaaatcatgc attccttgtg tgcccattga
     9961 accaccattt ttatttaaaa tggcacgtaa atttgcgaca gttcttgttg gattatctgt
    10021 ggcagtttca atgacaatag ctgtagcatg cggtcccatt gcctcataca ccacttcgct
    10081 aaagcctttt gtatccgtag atgaggcgcg ttttatggct gcttctatcc tatctttagg
    10141 catttgagct gctttggcat tttgtatagc tctacgtaaa ttggcatttg tatctggact
    10201 tggccctcca gattttattg ccatagcaat ttctcttcca gctcttgtaa atgccttagc
    10261 cattctgtcc cagcgagcaa acttggctgc ttttcgatat tcaaacgcgc gacccataaa
    10321 aatataataa tcttattatg tgaattaatt agaatgcaaa gataagcgaa tacttttttt
    10381 ttgcaaaaaa atataaacac taagtatgat tagcataatc cacattaatt tgatacttat
    10441 taattgcatt aaataaaaaa atgccaatca taaagattga catttgatat tggataaaaa
    10501 atacgcttaa gtaaatttca agtatttatc gtgaatactg cgaaatttaa aattagcagc
    10561 atctaataaa taattttgtt ttagcttcca aggatattct acgccttgac ttggaagtac
    10621 gcctgcttga atagcacgtt gtacatagcc agcaccaaag tttaaagctg gttctctttc
    10681 aattggctta tttgacttta ccacgaaggc attttttcct ttgcgttctg tttcgtttat
    10741 caatcggcag aagtactcgc ctaccatatc agattttaaa gtccatgatg aattcgtata
    10801 gcctaataca aaagcaaaat taggaatatc ttttatcatt aaacctttat aaacgatgcc
    10861 atcagatgct ttaaattctt tgccatcaat tgaagcttta ataccaccta acatttgtaa
    10921 ttctaagcca gttgcggtta caattatatc tgcttttaat tttttaccag attccaacat
    10981 gatgccatct tcgacaaatt tctctatatg atctgtgacc acatttgctt taccagatcg
    11041 tattgcttta aataaatctc cattgggcac tgcacacaat ctttcgtccc aaggattgta
    11101 ttttggtgta aaatgttttt caatgtcata gccttttggt agctggcgtt ttacatggtc
    11161 tctgaagtac attttcatta attcaggctg actttggcaa agattatagc tggtaacttg
    11221 taagcctatt tgtgcagctc tgtttaaacc atactgtact tttgtaggca aattctttag
    11281 taattgctga ggaattaagc ttctatttgg aatggaagca atatatgtcg gtgagcgttg
    11341 taacatagtt acactcttag ctttatcggc cattgccggc acgagcgtta cagcagttgc
    11401 accactgcca atgatgacaa cgtctttacc tgaatagtca taattctccg gccaaaattg
    11461 tggatgaatg atatcacctt taaatttctt ttccccttca aattttgggc gatgtccttt
    11521 ctcgtaatta taataaccca tacaagaaat gacatatttt gcagtaaacg tctttgtttt
    11581 atttgtttta ttatctgtgc aagtgatagt ccatctattt gtcgtactgt cccagtctgc
    11641 actattaatt ttagtgttaa aacgaatgtg cttatctata ccattttcac ttgcagtttc
    11701 tttgatataa ttcaagatta aaccaccatc agcaatagct tcgttatatt tccaaggttt
    11761 aaagctatag cctaaggtga acatatctga atcagaacga atgcctggat acctaaataa
    11821 atcccatgta ccacccaatg atggtctgtt ttctaaaatt atatatgatt tacctttgca
    11881 gttttcttgc aagtgatagg cagtgccaat accagatagt ccggcaccca taataataac
    11941 atcgaaagtg tttttttcca tatctaaata atttgataat tcaagataga atgactattt
    12001 ataaaatgca ctttactatg gataatttat taaataaaga caatctatta gtcgctttaa
    12061 taacaaatgt atatagaaat attaggcaaa aacaggtgta ttattcaatt gtttaattgt
    12121 tacaagaaac gactgcgcac atcaggacta ggcagcatgc atgattcttt cttaccaaac
    12181 catttatatc tattcttggc aataatatcg taggcaaaat ctgttaatga tgtaggtaga
    12241 atgctaaaca attgcaaaac gcgccagaaa ccaccaaaat agttcgctat tttaaatgct
    12301 gcttttgatt taaaataggc tatattattt tcaataaaaa caacgccttc aattgctgtt
    12361 gacagcttat aatgtatcaa taattgctgt ccgatttctg attgtaggct cgtaaatttt
    12421 aatgagttgg ttttatcatg tttgataata aactgtacac ttgcttcaca gaaattacaa
    12481 acgccatcaa ataaaactaa tttatcgtat tgttgtagat ttatgttact cattcttaat
    12541 aaattttgaa gtacctattt gatttgtatg tgtattcgta atctttataa cataaacgga
    12601 ggaaggtagt tcatgtatat caacatagcc tgactgttgc aatgtggttt gcatcaccaa
    12661 acttcctttt atatcaaata tttcaagcag tgtattgtca tttacacctt caataaataa
    12721 tttatctttt gcaggatttg gatacacttt tactgtgtag tttttagcag gtgtttcttt
    12781 tactggtgtc aaatatcctg gcaatgcttt tcccataact ggatttatca tcattgtacc
    12841 tggcaatcct gattctcgcc atttattggt gttgccttca acataataat tatatgaaga
    12901 acgaatatta tttctatcaa aaccaacaac taaactcagt ttattatcga caattaaacc
    12961 tacggcaaat gtatcgttca taattaaagg ataactcgct ccgtttttta caaattgtgg
    13021 cttaataggc gcataataaa agccatttat tgtatctaca ccatactcat ttaccaaatc
    13081 ttctattttt aaattcgttt cttgataaat taaatcattg tcattataca ccgtgtttct
    13141 tgtgaaattt ttccatacac acacactgaa aacagctagg tgattattcg gtttgacagg
    13201 gaaaaattgc aacttaatag cttgtaatgt atctggattt cttaaaccat atttaaccgc
    13261 cattttataa aaatccatgc ctgtatttgt ttttaaatca ccaacaagat aaccacgctc
    13321 agcagagcca tcatcatagg caaaatagtt gctaaacact tgttgatgcg aaaccgcatt
    13381 attcgccaat acttttgcag gttcaccagc ttcagcagag acattaaaat gataatcgac
    13441 atttataact acagtgtctc ctattaaatt tgttggaata ttgaattttg gataatcaat
    13501 tttatttaaa gtaattggac cgaaatcgcg cgaagggcca ctaaatgttg caattgtact
    13561 tgaagaagac acatctgttg cttcataaaa atcaacaata tctgtcgttg tattaatgaa
    13621 attattttta acaaataaac tcactgtatc tgctaaatca gtcgtatcaa attgattgta
    13681 cggcatcaca taatactgct tcaataaact tggtggcaaa aaagcatacg ccatttcttt
    13741 gatatttttt tccgatgcgg tatctctatt tttatcgagt tttacataat cgatatgcca
    13801 cgtatcattt gcaccattta agttgccaaa agactggaat ctaaattgaa aattagcatt
    13861 caaataaatg gaatccactt ttaaaaaaac ttgctgtaaa cttgcactgt ctaaagcggt
    13921 cacttgccaa atgatattcc aatttcctga tgtgtcttta tattgaaccg tcaataaatc
    13981 attaggtgca gcaggtgatt cgccaaaatc tccttgaata aaatagaaac tcaaaaaaac
    14041 attgtcagca ccagacaaac cagataaatc aattggattt gacgtcaaca tatctgctgg
    14101 tccatgcgtg gtattgcctg atgtattgta gggcacacca ctggcatttg tgccatcgaa
    14161 agttgctacg cctacggata ctgtttcttt gggaaatgtt gtattaataa acacaaaatt
    14221 atcttgccaa agtgcaggat tgggataaat atcttgctgg taaaaatctt caaagaatgg
    14281 taatgaaatt gcagaaattc tatgtgctac tttttgttgt aaaaatgatt gattcgtcga
    14341 tttattcatt ttcgtatgat aagttagtgg cgtcagagat tctttttgtg ccatcaactg
    14401 tattgtaatc aataaaaata aataggtagt aattatacgc atatcaatca aattatggat
    14461 tggtggtgtt gctatttttc tttggcacaa gtttattcat gccattgctt tctgctgctt
    14521 gaacttcagg ctctgcgata tcgctttttt gttggcgaat atataattga acagaattat
    14581 ttgcacctaa ttgtcctggt tgtccgaact ttggattttg gtcgtacaca taagccgaaa
    14641 gcgtgtcttc tattgggcca gctgcaataa tttcgccaac atttaaatgt actgcttcta
    14701 aaacagcacg tgcttccaat accgtcatgt ctgtaattgg tggcacttct gttacttgat
    14761 taccaaagcc atcgcctaaa accaaatcta ccatagtgcc tttaggcact accgttccag
    14821 gctttacaat tttgccattt acctgaatat tcaaaacagc atcttttgcc atatcaggta
    14881 tataaatcaa ttgacccaac tttaggcccc aactatttaa tattaattct gcctgacgtc
    14941 gtgagttgtc aataattgaa ggaattttaa cgcttggtgg tgctgatgca tttagagtca
    15001 aataaattct tctattttct tttacttttg cgccatttaa aggctgttgc tccaaaaccg
    15061 acaaaggtgg tttgctttca tcaaatgtag aatccgcaat gatataatct aaatcataat
    15121 ttttcagctc atttttagca tcatataaag atttgccttt cacattgggc acgactacaa
    15181 aatcaccatg atctgtgtac caatttaagc caaaatatac aaacaataaa agtgcaacag
    15241 atgcacccat tatatacagt atcgtcaatc cggtttttcc catcaaataa gctttacttt
    15301 caacgtcaaa tatatctatt tatgttgtaa cccacatttt ttttaaataa aaaaagcaca
    15361 tttattgttc agatattgtt agtaactcta tatttacagc ataattacac ttgcatgcaa
    15421 aaacgaaaaa ttggattagt atttggagga aaatcaccag aacacgaaat ctctatcatc
    15481 tctgctcgaa atatttataa tgctatcaac aaagaaaaat ttgaagttat tttaattggc
    15541 attgcccaag atggaaaatg gtatttagaa gatgcgttga acctgcaaga tgaaaaatgt
    15601 gtcattggaa aatcaggcat gcaattggcc gtcgtttttg gtaatgagca gcataaatta
    15661 atccgcttag acaatcaaca atatatatca gatgtagaag ctgtatttcc gataacacat
    15721 ggcccaaatg gcgaagatgg cacattgcaa ggaatatttt tacaactcga tattccattt
    15781 gttggcccaa gtgttttagg ctcggcagta gccatggata aagattttac caaacgtatc
    15841 ttgcgagatg ctggcattgg ccatgcaaaa ggcattacaa tttataaaca ccaaatcgac
    15901 aatatttctt acgatgctat tgtggctgag ttgggcaata cgctgtatat caaaccttgt
    15961 aacatgggct cgagcgttgg cgtttcaaag gctacaaatg aagctgactt taagaaatct
    16021 attgccgaag cgtttaaatt cgaccataaa gtattggttg aaaaagcaat tattggtcgt
    16081 gaattagagt gtgctgtttt aggaaatgaa attatagaaa caagcacagt aggagaaatt
    16141 gtgttagaaa aaggttttta tgattttgaa aataaatata aaaacgacaa tgcaaaaatc
    16201 tatataccag cgcccaacat ttctgctgtg caattagaaa aaatacaatc tactgccaga
    16261 gatgcctatc aagcattagg attagaagga ctttcgagag tcgatgtttt tttaacagat
    16321 aatgatgaag ttatcatcaa cgaaccgaat accttgcctg ggtttacgtc tatatctatg
    16381 tatccaaagc tttgggaaag ctcaggtttg aaatatacag atttgattga gaaactcata
    16441 gaattgtcga ttgaacgcgc tacacgcgag aaacaattac agcgcgtccg actcgtttaa
    16501 ataatcgatt ttatccgttt actaagtata attacctttc aaacaacaaa tatcatagtt
    16561 gtgtgtgttt tgctcaaatt tacagcaaca aacaccataa catgaaaaaa gtaatcctct
    16621 taatgttttt atccataatc gtgataaata catttgctca aaaaagaaat gaaagtaaaa
    16681 gcccaatcat aggaagttgg aaatttacgc gacaatcaac actcaatgat tttcaaaaag
    16741 tatttaaaga aaaaagttac caagctgagt tatttacatt ttctggcaat caaaccttta
    16801 aacatgaatt tgtagatgca agtgagcagg tcattaaagt attgaaaggc aaatggaaaa
    16861 ttgcaaacga taaaatccaa attaaatatg ctgatgtcga ttatgaagta tatatcgatt
    16921 attttttctt agaaagtgat ctagtattag gacaaaattt cagccatgta attttcaaaa
    16981 agctggataa tgaagaaaga aatttgacga tgaaataaaa tataagcaat atttcatcat
    17041 aataaatgaa tttctactca aaaaatgtta aaaaatatcc atttactcat tcaaacctac
    17101 cgtttactaa ctatatattg acatatgtca agaaaatgag caattttgat gtaccataaa
    17161 acattaaaat aaagcatatg aaaaaggcat tttttctaat gtgtttagta gcgataagcg
    17221 ttacagcatt agcacaacga aaaagtgaaa caaaaaaccc ccttattggc acttggaaat
    17281 tcaccaacca gactaaggtg aatgaattcc aaaaagtatt tcaaaataag aacgattttc
    17341 cgattgagta ctttacgttc aatgcaaacc aaacattcaa acatgaattt ttagatgctg
    17401 atgaacatac cataaaagta ttgactggaa aatggaaaaa aggaagcaat tcaagcatca
    17461 ctatctacta taaagatatt gagtttgaat tgaacactgc ttattttttc atagacaaag
    17521 acttagtgct tggacaaaac tttagtcacg ttattttttc aaaacaagat atgtttgata
    17581 aaaatatcgc tatgaaataa gaatacaagt tgaatattca attgcatcgt gtatgcaatg
    17641 tggttaggat atactataat tgtttctatt ctgttcacta aaaaaatgaa aaaaaaatgc
    17701 cgtgacggat gcttggaata cagcatccgt tttgatttgc aacactttta ttttcaatat
    17761 ttcttgaaag tttaaaaaca acaaaattct aaacttaaca tgtattacac gtaatgaacc
    17821 atagttttac gattaattta caatacctgt atgtatatgt atttaagttt aaatttcaca
    17881 ttccattaac tgctcattta cacgaaatac atttttatat caaactttag taattggatt
    17941 tatttgacaa atgtcaaaca actgacctaa atttgcagta tcaattttga taaaaaaata
    18001 aatcttttta aaaatggaat tcacacaaaa attcaaagaa gctgttgaca gcttaacagc
    18061 agaaacaaaa gcaaatgtaa cagaattacg ttacaacgta gaaaacactg taaaaaacgc
    18121 agtaagcgaa gctaaagacg aagtagaatt acaacgtaat cacttcaaat ctacagctga
    18181 aagaatccaa gctaaattga aaggaccttt cactttagac aaagttcaag ctgatgttaa
    18241 agaagaagct aacattttag tggctgactt aaaaactact ttcaacagaa atgctgacag
    18301 agtaaaaggt ttgttcaacg aagcatctgc aaaagttgaa aaacaagtag aagaagtgaa
    18361 agaagaagtg aaaaaagcta cttctaaaaa aactaaagca acagcgtaat cctgttttat
    18421 agattacatt tttaaaaaga gccgccaatt agagcggctc tttttttatg cttgaattga
    18481 tattatttaa attaaatacc tttgtacaaa ataaaaaaat gaaaattgct ggttatatag
    18541 aacatcctat tgtaaaaatt acagtgttac acatgaataa cagatacgtt ataaaagtag
    18601 aagctaacat gatggagcaa acatacaaat ttcatgaaga cgacaagcta cgcactatgg
    18661 ctgatatgga aaaattagtt gatgaaacat ttatcaatga atgcatcaag cgctttgcag
    18721 aaatgaacaa aagtcgagga gaaatgtatc aaagaaatat actgtaaacg caaaaatcag
    18781 tattattact atgctattgc atcgtcatac attgacttac tgaggctgat ttcttgtgct
    18841 ataacattgg cagtttctct gccagagcgc aacgctgatt ccatacttcc aacagaaaaa
    18901 caatcgccac aagcatagac attatcgcta attgcttgca caaatttgat ttcatctaaa
    18961 attggtttga atggcatagc atattttata tgataggttt ttaagtattg ccaatccatt
    19021 acttttagac caaaccattc tgataattct gtcaggcatt ttgctactaa ttcttcatca
    19081 tctaaatcgt gatctttaac gacattgaca gctactaaat gtttgccttc tggtgcataa
    19141 ttgctatgca ataatgttgg aacaaaaaca tgatttacta aagtagtacc attattacca
    19201 tttaaaataa caacaggttt acttattggt gcatgttcag tagaaaaata aagtgtcgat
    19261 acgtgcgaac
//
LOCUS       DAOQQV010000042        19053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_875_length_19053_cov_7.820823, whole genome shotgun sequence.
ACCESSION   DAOQQV010000042 DAOQQV010000000
VERSION     DAOQQV010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19053
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_875_length_19053_cov_7.820823"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1352)
                     /locus_tag="PLL59_06040"
     CDS             complement(222..1352)
                     /locus_tag="PLL59_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY23401.1"
                     /translation="MKIAIVGGGIGGLTTALALKQNGQDVTIYESASEIKPVGAGILL
                     AINAMQVYKNLGIHKKIEKAGAIVSNIQTTDAQLNTISETKLSHFENKYGVCSVAIHR
                     ADLHTILADEIGWENIKLSKRLINIEEGNEFKLHFEDGSSINCDVLIGADGIKSVVRK
                     QLFNEGTFRDTKQRCWRGVCEFDSTAKYNQIAIESWGKGKRFGFVEIGKNKVYWYAVI
                     KDTMMKENYDLADLFNEFHPEIVKIINKTPKDNIIFSDIKDLQPINQWQKGKVCLVGD
                     AAHATTPNMGQGACQAIEDAYVIGKLFGQEKSVEAVFTQYEKLRLEKAHYIINTSWTI
                     GKVAHYENSIAIWVRNFLVKNTPINIVEKQLNKVFSIEYNIV"
     gene            complement(1486..3276)
                     /gene="lepA"
                     /locus_tag="PLL59_06045"
     CDS             complement(1486..3276)
                     /gene="lepA"
                     /locus_tag="PLL59_06045"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HMY23402.1"
                     /translation="MENIRNFCIIAHIDHGKSTLADRLLQITNTVSERDLQAQTLDDM
                     DLERERGITIKSHAIQMDYFQDGKKYIFNLIDTPGHVDFSYEVSRSLAACEGALLLVD
                     ATQGIQAQTISNLYLAIENGLEIIPIINKIDMDGAMVEEVKDQIVDLIGCKHEDILEA
                     SGKTGLGVDKILPAVIERIPAPKGDANAPLQALIFDSVFNSFRGVIAYFRVINGTIKK
                     GDKVKFVNTGKEYHADEIGIMRMTPEPKGEVSAGDIGYIITGIKVSREIKVGDTITHV
                     QRPCIESIKGFEDVKPMVFAGIYPIDNDDFEELRDSIEKLQLNDASLVWEPETSLALG
                     FGFRCGFLGLLHLEIIQERLEREYDMTIITTVPNVTYYCYTKQGEKITVNNPTDLPDP
                     TYIDYVEEPYIKAQVITKPEYVGPIMKLCMDKRGILKNQVYLSPTKIELQFEMPLAEI
                     VFDFYDQLKSISKGYASFDYHMHDFRESDLVKLDIKLNGDNVDAFSALIHRSKAYHFG
                     SRLCEKLKELIPRQQFVIAIQAAIGAKIIARETISAMRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMRQVGNVEVPQQAFMAVLKLND"
     gene            complement(3327..4199)
                     /locus_tag="PLL59_06050"
     CDS             complement(3327..4199)
                     /locus_tag="PLL59_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23403.1"
                     /translation="MHTAIFSFAAGEIRFAGAKQAGVGMNSVCMVNVYSAYNNQAAGA
                     YLERPSFGIYYAPVFIGQGVSNVTGIFAMPVKKGGTIGLSVNYFGYNLYNEKKVGLSY
                     AIKLAKWMAIGMQLDYLNTKISGYGSKNNVTFELGLFSKPIDELSIAFHVYNPLKLYI
                     DKATGERIPTMFRIGVTYEALKKFFISVQFDKDLTNKVIFRTGAEYTLKDIVSFRAGI
                     ATTPVTGTLGLGVLLKQGVGFDFAFSYQANLGFQPHFGLVYSFMKKKPDLQQPATSAP
                     KQKKLLQRRLADKH"
     gene            4445..5587
                     /locus_tag="PLL59_06055"
     CDS             4445..5587
                     /locus_tag="PLL59_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMY23404.1"
                     /translation="MKKIILLSLLVFSVIKLSNAAAFLHNEYAYLQEITFELYTDEVP
                     AYKIPEQLKSIEEILMFEVLHATWKDKHWVAYTLNKNLLGTFKVGQPALMDNGKNKKI
                     FFIASFPGTVGGLDIYTSEYENGKWSNPKNLGKEINTTKNESNPGLLNENTLTYSSGG
                     IIKKLNLKTFAVVDLEEPKAAEIVKPEIKSTPTNTGATKNNKEEIAAIGDNSTALPAG
                     STVPKDSDYGAIGDNSTAAPVMSKESKNAIEKQTKAIEEKIVVEEKAKKEVTMQSAPK
                     STMATSSVVNTSTTMSSGISSIGVQSRDAMLAKYKTAIQLGAFSAPKWEQFEPLQKLG
                     KIITYKNEKGANVVWLVGFANRAAAEQILPQVKAVPGFEDAYVTGK"
     gene            complement(5659..12393)
                     /locus_tag="PLL59_06060"
     CDS             complement(5659..12393)
                     /locus_tag="PLL59_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HMY23405.1"
                     /translation="MKKFLQFLLLCLLLFIASKAEAQNNNCANAITVCNNQLAEQLND
                     GVGTQECPSGGCGCMLAGEKNTRWFRIVIQTGGTLEFTIRPYNGSADYDFSLWNRGVG
                     GTCPSGGTLGTPDRCNFAAPASPTGIRGTSNGNSNGASGNLYSNNMTVTAGQVLYLLI
                     DNYDGTPEGFYLDFFGGAPGSGTGTTATFSCSGVNQCTTCNDADCKTYYFASPDDYSF
                     DETAANGACHSNFAYASVKTSTVCGTFNVPAPYTTVQFPTNRGYEITTTNGANTTTCL
                     NSANISYQVWDNCAVAPLTPSSPGIYNGLNNLSTYKVCKTITVSGADCWLSRVCLPYW
                     TEVANDVICNASPLTVNAAPINGTNAGASSDLDAGCTGYQDVYYSFVAPASGRVQINV
                     DPNAASDVKINIIGPMAGLDEGINDCNLSCSQLGENNVVSDCNDYAGRDGVERLFAFV
                     IPGQTYYVWVSGTFSYPTANFTIQVSETLTSSAIPQPGPELTNNSGLPDAIPSNDLCT
                     NATNLNLCVAQAGTTIGATAGCTDPDPEYVDALTLENDVWYRWTAPANNGNSEVTLTV
                     TGISCSGGMDGSTGIQFGIFSGSCATLTPISQGTTSVTFTPIAGQTYYFVIDGNAGAQ
                     CNFNIEVKRPIVSSQTCNPASSCAGSALSASMGVTYYGSNPGTRWAYCKSNTFGTPCN
                     TINLDDPNTYSIYNPSVGLPDPGCTPATYTFIGYILADNGASTIPGFPSYPLPQPATT
                     NCTRATSACTFNIYPRLSNYITYTANGCSQVVTINPSCPAALSVTGGALSQSVAVGTV
                     GGNFTAVNVAWNPPYNTGSPAACNTLSIQTPIYCPGPTSDAPCIARPLTVGAPALASD
                     NISAVYNANEQVSCTANSAGYGVWFSFITPPSGNADINLTAVGTSNNLDAQIYWLSSR
                     RASVFDCSIGQLTSDVCSSCTEFNTDCNIVDHGGFCADAGGVNANETLQLRGLFPGET
                     NYIMIDGYDSPTASERRGNFSIRVVDAGGGPTRPSNDNCSGAIDITNIACGKIPASNK
                     NATSLCNGDPSFTPGSTENSVWYYYVPPVTGSYTISYANAKGYDCLGHIQNSLFPFPS
                     SANGAGIQFALYTSSTNNCNGTFSELPTSAISTGALNGQVTLNLTGGQKYYILIDGFL
                     GFQCDFEFKINSNLCCSADLGAVEKSDTVLCYGERMTLGVTADPINFGSNAEDNPVVA
                     WLFSPTEPTGAALDPFDPSNTSLTYLVGQTDIITPGTAYTDSTKVYKENIVRSPAGGY
                     GDISYGYWDTPIYKDIAISGFPAGATLQNVNNIKVCVYFGFEDIGSHDVQLVSPSGAT
                     IDLFSDICNESSGGMSVCFTSTAPTTIGSSCLAIGGVPQFGALNNELTGDFRPEASFA
                     PLVGTALNGTWRLRIDDDDLDNEGFWYFGWTIDLVHTQTSIGPPSIGPRHGDLTIVNN
                     DPFKYGPQEFWLTPVTMIDYDPNTDLLTNDPTCYDYGPPTKVTLLERVTTPTITPTCA
                     APGDGSNGISLTLTSPTGGLPGLPSAPSSNTLSFSNTSSLAIPDNSASWSNRDINVSG
                     FTAGATLTANTNINVCLTFNPEHTYASDVVAQLVAPNGGILQLTPGSGANGNDLNGQY
                     CFAISGTALLEAGVASGGNINTGIYDWSGNYNNINGTLLNGLWRLQIRDDAGGDVGTL
                     ASWSIALTGLTPGVSTAAYTVTGIGAASSISFPSPPVQEAEVSNPFTVADGQAWNVRF
                     RDAAGCESEIGGTYNQPNLGDLTLDTSVCDGAIDIAFSTSNPIPLFSQYRVLLDFDSY
                     PQDVSWFIYDGSNQVIASGGGYGTSVGASTFTTTATINPNNGPFRFVLYDGFDDGFGS
                     GGGSSNNGGNTSLNYIRIMELHANGSVDTLFSQNYAYCSPIYCVGPAASLFGQLDVNL
                     GTPIGTFATGTAVTLENDRTCTGTTVAGAITMNTSGSGTINTTAAGVNPGSSYSVQYS
                     YTDQYGCTKSICGPIDVFPPVSINPTINCTNPPTVNVNASCPNCNATYVAEYSYNGGL
                     SWTTATSANFQEIYTFARVRNTVTGRISCEVSSVKLADCPTVLPIELISISARAINNE
                     FIQVSWSTATERNTNKFEILRSTDAIQFTKVGEVAAAGNSNNIRQYKYDDFDVAPGIV
                     YYYRIKEIDNDNRHQLTNIVHAKINGASFEVVSIHPNPFVDNTTITLYTEEEREVNLK
                     VFNDLGELLKHEQKQLTIGLNEWNIATEEWAKGVYYFIINNGEKPLTKQVIKLN"
     gene            complement(12396..13010)
                     /locus_tag="PLL59_06065"
     CDS             complement(12396..13010)
                     /locus_tag="PLL59_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23406.1"
                     /translation="MYKILHILLIIFFFSCNYFSIIAQENRINNFKKTNTENNIKTKS
                     NTEVLGINKAEIQENLKGINTNNTTTQLRINDSLSIQKIQTEKGINNLEISIQNNTAA
                     TENASYTKVTFSENEKTTNTERSYTKIKPSINYVPKQVKADIHPIVDYNSAPVNPNKK
                     DYLLQEANDLQREIELNTNNPNYELEKNTRKLEEIRDLIEKTNK"
     gene            13158..14162
                     /gene="trpS"
                     /locus_tag="PLL59_06070"
     CDS             13158..14162
                     /gene="trpS"
                     /locus_tag="PLL59_06070"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HMY23407.1"
                     /translation="MKERVLSGVRSTGNLHLGNYFGAIKNFIYMQDNYEAYFFIANLH
                     ALTTHPNPELLKKSVRATLVEYIACGLDPEKSTIFVQSDIPEISELYALLNMLAYKGE
                     LEKCTSFKEKIKKHQENINAGLLTYPVLMAADILIHRAHKVPVGKDQEQHLEMTRNFA
                     VRFNHLFGVDFFPEPQAYSTSGKLVKVPGLDGTGKMGKSEGEGNAIFLREDAESIRKK
                     VMRAKTDAGPTQANQTKSEEIQNLFTLLDLVSTPDTVSYFEDKYNDCTIQYGYLKKQL
                     AEDIINFCAPLKEKIIALENDHAFINKIAKDGAEKARVSARATLDGVQEIMGLKPIWK
                     "
     gene            14351..14989
                     /locus_tag="PLL59_06075"
     CDS             14351..14989
                     /locus_tag="PLL59_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMY23408.1"
                     /translation="MAKQKKQLKHIAVAGNIGSGKTTLTEKLAKHYNWEARFEDVENN
                     PYLNDFYQDMPRWSFNLQIYFLNSRLRQLVEIQEGTQTVIQDRTIYEDAHIFAPNLHE
                     MGLMSTRDFENYISFFRTISKKVNAPDLLIYLKASLGTLVGQIERRGRAYEDNIRLDY
                     LKQLNQYYDNWIENYKEGPLLVINVDDLNYADKNADFGKVVEKIDAELFGLF"
     gene            15189..16481
                     /locus_tag="PLL59_06080"
     CDS             15189..16481
                     /locus_tag="PLL59_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HMY23409.1"
                     /translation="MSNYKFETLQLHAGQEADPTTGARAVPIYQTTSYQFKDTEHAAN
                     LFGLKEFGNIYTRIMNPTTDVFEKRIAALEGGAAALATASGQSAQFLALNNILQAGDN
                     FISTPFLYGGTYNQFKVAFKRLGIDARFAEGDNPASFEKLIDDKTKAIYLESIGNPRG
                     NVPDWAAFKALSDKYDIPIVVDNTFGAAGYLFQPFKHGAHVVVASATKWIGGHGTSIG
                     GVIVDGGNYNWGNGKFPQFTEPSEGYHGMVFWDVFGDKGPFGNIAYIIRARVEGLRDF
                     GNAISPFNSFLLLQGVETLSLRMERHQQNAMALAQWLKEHPKVASVTYTGLPDSPYHE
                     LAKKYLTNGFGSLFTFEVKGGYDAAKKFINSLQLTSHVANLGDAKTLAIHSASTTHEQ
                     LTDEEQIAAGVSKSSIRVSVGIEHIDDIKADFDQALNA"
     gene            16592..16891
                     /gene="gatC"
                     /locus_tag="PLL59_06085"
     CDS             16592..16891
                     /gene="gatC"
                     /locus_tag="PLL59_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HMY23410.1"
                     /translation="MIVDNALVDKLANLSKLEFDAESKEAIKQDLSKILNFMEKLNEL
                     KTDGVEPLIYINEEPNASRADEVNYPITKAEALMNAPLHNEDFIKVPKFVKGTKY"
     gene            16941..17657
                     /locus_tag="PLL59_06090"
     CDS             16941..17657
                     /locus_tag="PLL59_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMY23411.1"
                     /translation="MSNAVINLIDIKRAFHLGDEVIHALKGINLSINTNEYVALMGPS
                     GSGKSTLMNLLGCLDTPSAGQYLLDGIDVSKSTDNELAAVRNKQIGFIFQTFNLIPRL
                     TTIENVALPLIYAGWNKSDREARAIEVLHQVGLGDRMKHKPNELSGGQRQRVAVARAL
                     VNRPSLILADEPTGNLDTKTSYEIMRLFEEIHANGNTIVVVTHEEDIAMYAHRIVRMR
                     DGLIESDKLNETIKKATAAL"
     gene            complement(17705..18289)
                     /locus_tag="PLL59_06095"
     CDS             complement(17705..18289)
                     /locus_tag="PLL59_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HMY23412.1"
                     /translation="MPIDLKELSTVTLILFAIIDILGSVPVILDIKSKVGQVDAKYAT
                     GVSGGIMIVFLFLGKELLNLIGIDVASFSLAGSIVIFLIGLEMTLNRDIFKSGNSTPK
                     STSVVPIAFPMIAGAGTLTTLLSLRSEYHVQVIMIAILINLAFIYIVIRFIPKIEHLL
                     GDTGINISRKIFGVILLSISIKLFSKNLAILFHF"
     gene            18550..>19053
                     /locus_tag="PLL59_06100"
     CDS             18550..>19053
                     /locus_tag="PLL59_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23413.1"
                     /translation="MKKGIVLLCALLVLASSVSLGAKKPPKSNKSAVGNNRFSLGIGM
                     EVADYISPANKKVTTFKTPVSFGPRFTFWYNPNSSVAVGADLGSHAFSSKTDASATAI
                     NTYNVLYAAVVAYKFNNGYILKEDAAVSPYLFAKLQGSWAETPLYKESVHGFGIPIGA
                     GINFKVAN"
BASE COUNT         6043 a         3444 c         3486 g         6080 t
ORIGIN      
        1 aatttctcta ttcttataat acaacgtaat cttccgaaca cttatggtgt cctcacgaac
       61 cattactatt gtggtcgctt tcaaagtcaa aagtttctta aaagtcaaaa cctaatttag
      121 tcaacaccat gctaccatgg tttgtgtcct caagaaccat ggttaaggct aatgttgaat
      181 tttgctatga actcgacatt tccaaactgc agttagcagt atcaaacgat gttatattca
      241 atactgaaaa ctttgtttaa ttgtttctca acaatattta tgggtgtgtt ttttaccaaa
      301 aaatttctta cccaaatagc aatactattt tcataatgag ccactttgcc aattgtccag
      361 ctcgtattaa tgatatagtg cgctttttcc aaccttagtt tttcgtattg agtaaaaacg
      421 gcttcaacgc ttttctcttg tccgaataac tttcctatta cataagcatc ttcaatagct
      481 tgacatgcac cttgccccat attaggtgtg gtggcatgtg cagcatcgcc tactaaacaa
      541 acttttcctt tttgccattg attgattggt tgcaagtctt taatgtcgct gaatatgata
      601 ttgtcttttg gcgttttatt aataattttt acaatctcag gatgaaattc attaaataaa
      661 tctgcaaggt cataattttc tttcatcatt gtatccttaa ttacggcata ccaataaact
      721 ttatttttgc cgatttctac gaacccaaaa cgtttgcctt ttccccacga ttcaatggct
      781 atttgattat acttagcggt cgaatcaaat tcacaaacac cacgccaaca tctctgtttt
      841 gtatctctaa atgtaccttc attaaataat tgttttctaa cgacagattt aatgccatca
      901 gctccgatta gtacatcaca gttaatactt gagccatctt caaaatgtaa tttgaattca
      961 tttccttcct caatatttat aagcctttta gatagtttga tgttttccca tccgatttca
     1021 tctgccaata ttgtatgcaa atctgcgcga tgtatcgcca cactacatac gccatatttg
     1081 ttttcaaagt gacttaactt tgtttcagaa attgtattga gttgcgcatc tgtagtttgg
     1141 atatttgaaa cgatagcgcc tgccttttct atttttttgt gaatgcctag atttttatat
     1201 acttgcatag cattgattgc gagcagaatg ccagcaccaa caggtttaat ttctgacgca
     1261 ctttcgtaaa tagttacatc ttgtccattt tgttttaaag ctaaggctgt tgtcaaaccg
     1321 ccaattccgc cacctacgat tgctattttc atgtgtattg ctgtttttag tgtaaaccta
     1381 tttcaagacg agacaaagaa atacgtcgaa gaaaaacatt gttcaaagca aaattaagtg
     1441 aataatttcc gcagttctaa atgaacacca actaaaaact ttattttaat cattcagttt
     1501 taaaacagcc ataaacgctt gttgtggcac ttctacatta cctacttggc gcatgcgttt
     1561 cttgcctttc ttttgcttct ctaataattt tcgtttacga gaaatatcac caccataaca
     1621 ttttgcagtt acgtctttgc gcatagcaga gattgtttcg cgtgcaataa tttttgcacc
     1681 tatcgctgct tgtatggcaa ttacgaattg ttggcgcggt atcaactctt ttaacttttc
     1741 acacaagcgg ctgccaaaat ggtaagcttt gcttctatgt atcaacgcag agaatgcatc
     1801 gacattatcg ccatttaatt tgatatctaa ttttaccaaa tcactttctc taaaatcgtg
     1861 catgtgataa tcaaaacttg cgtaaccttt agaaatagat tttagttgat cataaaagtc
     1921 aaatacaatt tctgctaatg gcatttcaaa ttgtaattct atttttgttg gcgatagata
     1981 cacttggttt tttaagatgc cgcgtttatc catacataac ttcataattg ggccaacata
     2041 ctcaggtttg gtaattactt gtgcttttat gtatggctct tccacatagt caatatatgt
     2101 tggatcaggt aaatctgttg gattgtttac cgtaattttt tcaccttgtt ttgtgtagca
     2161 ataataggtt acgttgggaa cggttgtgat gatggtcatg tcatattctc gttccaatcg
     2221 ttcttgtatt atttctaaat gcaacagacc taagaaacca catcgaaaac caaaacctaa
     2281 agctaatgat gtttcaggtt cccaaactaa tgaagcatca tttagttgta atttttcaat
     2341 ggaatcgcga agttcttcaa agtcgtcatt gtcaattgga taaattccag caaaaaccat
     2401 cggttttaca tcttcgaacc ctttgatact ttctatgcat ggacgttgta catgtgtaat
     2461 tgtatcacca acttttatct cgcgtgatac tttaatgcct gtaataatat agccgatatc
     2521 gcctgcgctg acttcgcctt ttggctctgg cgtcatgcgc ataatgccaa tttcatcagc
     2581 atgatattct ttcccagtat tgacaaattt tactttatcg ccttttttta ttgtgccgtt
     2641 tattacacga aaataagcga taacaccacg aaaagagttg aaaacagagt cgaagattaa
     2701 tgcttgcagt ggtgcattgg catcgccttt tggagcagga attcgttcaa ttaccgcagg
     2761 taatattttg tcaacgccta atccagtttt accacttgct tctaaaatgt cttcgtgttt
     2821 gcagccaatt aaatctacta tttgatcttt tacttcttcc accatggcgc catccatatc
     2881 aattttattg ataattggaa ttatttccaa tccattctcg atggctaaat ataaattaga
     2941 aatagtttgt gcttgaattc cttgcgtggc atcgactaat aacaaagcgc cttcacaggc
     3001 agctaaagag cgtgaaactt cataagaaaa atccacgtgg ccaggtgtgt caattaaatt
     3061 gaaaatgtat tttttgccat cttggaaata gtccatttgt atagcatgac ttttaatggt
     3121 aatgcctcgc tcgcgttcca aatccatgtc atctaaagtc tgtgcttgta agtcgcgttc
     3181 agatacggta ttggtaattt gcaataaacg gtcggctaat gtactttttc cgtggtcaat
     3241 atgtgcaata atgcaaaaat ttcgaatatt ttccatcttg caaaagtaca gatattcaac
     3301 gtattcttga aatggtaaat aatttattaa tgcttgtcag ccaaacgtcg ttggagtaat
     3361 tttttctgct ttggtgcgct tgttgctggt tgctgtaagt ctggtttctt tttcataaaa
     3421 gaataaacga gtccaaaatg aggttgaaat cctaaattcg cttgatagga aaaagcaaaa
     3481 tcgaagccaa caccttgctt taacaaaacg cctaagccta aggtaccagt gactggtgtg
     3541 gtcgcaattc cagctctaaa actgacgatg tcttttaagg tgtattctgc gcctgttcta
     3601 aaaattactt tattagtcaa atctttgtca aattgaacag aaatgaaaaa tttctttaaa
     3661 gcttcatagg taacacctat tctaaacatg gtcggaatgc gttcgccagt agctttatct
     3721 atatatagtt ttaatggatt ataaacatga aatgctatgg aaagctcgtc aataggttta
     3781 gaaaataaac caagttcaaa tgtgacatta tttttactgc catagccact gatttttgta
     3841 tttagataat ccagctgcat gccaattgcc atccattttg ctaatttaat ggcataagat
     3901 aatccaactt ttttttcatt atatagattg tagccaaaat aattaacaga caaaccaatg
     3961 gttccacctt tttttactgg catcgcaaag attccggtta cattacttac gccttgtcca
     4021 atgaacacag gcgcataata aattccaaag gaaggacgtt ctaaataagc accagcagct
     4081 tgattgttgt aggctgaata tacatttacc atgcaaacag agttcatgcc aacacctgct
     4141 tgcttagcgc ctgcaaatct tatttctcct gctgcaaaag aaaaaatagc agtatgcaaa
     4201 agggcaactg tacttactat tttattaata ttatttctta tgtcttaatt gctttgttga
     4261 aataataaat atctcacatt cactagaatg ggaatgctaa gatacaacat tctcttgcaa
     4321 aataaatgct tggcatcaaa cttgcacgct taagttgtaa tacaatattg tggaaaactt
     4381 tacgtattaa tagtggttta aaataccact tttgttgttt atgattttga aatatttata
     4441 cattatgaaa aaaattattc tacttagttt gttggttttt agtgtgatta aattgtcgaa
     4501 tgcggcggct tttctacaca acgaatatgc ttatttgcaa gaaattacct ttgaattata
     4561 tacagatgag gtgccagcct ataaaattcc agagcaatta aagtcaattg aagaaatctt
     4621 gatgtttgaa gtattgcatg ctacttggaa agataaacat tgggtggctt atacgctcaa
     4681 taagaattta ttgggaacat ttaaagttgg tcagccagca ttgatggata atgggaaaaa
     4741 taaaaagatt ttcttcatcg catcttttcc tggcacagtt ggaggtttgg atatctatac
     4801 atcagaatat gaaaatggaa aatggagcaa tcctaaaaat ttaggcaaag aaattaatac
     4861 gactaaaaat gaatctaatc caggtttgtt aaatgaaaat acactaacct attcttctgg
     4921 tggtatcatc aagaagttaa acttaaaaac atttgccgtt gtagatttag aagaaccaaa
     4981 agcggcagaa attgtaaagc ctgaaataaa atcgactcca acaaacacag gtgctactaa
     5041 aaacaataaa gaagagatag ctgcaatcgg cgataattct actgcattgc ctgcaggaag
     5101 cacagtgcca aaggatagtg attatggtgc gatcggcgat aactcaacag ctgcaccggt
     5161 gatgagtaaa gaaagtaaaa atgcaataga aaaacaaaca aaagcaattg aagaaaaaat
     5221 tgttgttgaa gaaaaagcaa aaaaagaagt gacaatgcaa agtgctccaa aatctacaat
     5281 ggcaacatct tcagtagtaa atacatcaac aacaatgtca tcaggtattt catcaatagg
     5341 tgttcagtcg cgcgatgcga tgttggcaaa atataaaacg gcaattcaat taggagcatt
     5401 ctcggcacca aaatgggagc aatttgaacc acttcaaaag ttaggaaaaa ttatcaccta
     5461 caaaaatgaa aaaggcgcaa atgttgtttg gttagtaggc tttgctaatc gtgcagctgc
     5521 cgagcagata cttccacaag tgaaagcagt gccaggtttt gaagatgctt acgtaacggg
     5581 aaaataataa tacttaaaat gaaaaagcaa aaggttgacg taataatgtt aacctttttt
     5641 tttattcttt ttgttttttt agttcaattt aatgacttgc ttagtgagtg gtttttcacc
     5701 attattgata atgaaataat atacaccttt tgcccattct tctgttgcaa tattccattc
     5761 atttaaaccg atagttagtt gtttttgttc gtgttttaat aattcgccta agtcattaaa
     5821 tactttcaaa tttacttctc tttcttcttc tgtgtaaagc gtaattgtag tattgtcaac
     5881 gaatggatta ggatgaatac ttacgacttc aaatgacgcg ccatttattt tagcatgaac
     5941 aatgttcgtc agttgatgtc tattgtcatt gtcaatttct ttaattctgt agtaatagac
     6001 aatgcctggt gcaacatcaa agtcatcata tttatattgt ctgatgttgt tagaattacc
     6061 agcagcagca acttcgccta ccttagtaaa ttgtatagca tctgtacttc tcaaaatctc
     6121 aaatttattt gtgttgcgtt cggttgctgt tgaccaagat acttgtataa attcattatt
     6181 aatggctctt gcactaatag agattaattc tattggtaat acagttggac agtctgccaa
     6241 tttaacactg cttacttcac aagagattct tcctgtaact gtatttctta cgcgtgcaaa
     6301 ggtgtagatt tcttgaaaat tagcagatgt tgcagttgtc caactcaagc caccattgta
     6361 agaatattct gctacataag ttgcattgca atttgggcaa cttgcattta cattgactgt
     6421 tggcggattg gtgcagttaa tcgttggatt tatcgaaact ggtgggaaaa catcgatagg
     6481 gccacatata ctcttagtgc aaccatattg atctgtataa gaatattgta cagagtacga
     6541 tgagccagga tttacaccag cagcagtagt gtttattgta cctgagccac tagtattcat
     6601 tgtgattgcg ccagcaacag tagttcctgt gcaggttctg tcgttttcga gcgtaacggc
     6661 tgtgcctgta gcaaaggtgc cgataggcgt tcctaaatta acatctaact ggccaaataa
     6721 tgaagcggca ggtcctacac aataaattgg agagcaatat gcataattct ggctaaataa
     6781 tgtatcaacg ctaccattag catgcaattc cataatacga atataattca aactcgtatt
     6841 tcctccatta ttactactgc caccaccaga gccaaatcca tcatcaaatc cgtcgtataa
     6901 cacaaagcgg aatggaccat tatttgggtt aattgttgca gttgtagtaa atgtgcttgc
     6961 accaacggaa gtaccataac caccgcctga tgcaattact tgattgcttc catcgtatat
     7021 aaaccaagat acatcttgtg gataggaatc aaagtctaac aacactcgat attgtgaaaa
     7081 taaaggtata ggatttgatg tcgaaaaggc tatgtcaatt gcaccatcac atacgcttgt
     7141 gtctaatgtc agatctccta aattaggctg gttgtatgtg ccacctattt cactttcaca
     7201 gcctgcagca tctctaaatc taacattcca ggcttgtccg tcggcaacag taaatggatt
     7261 tgatacttca gcttcctgaa ctggcggcga tgggaacgaa atagatgagg ctgcgccaat
     7321 tcctgttacc gtataggcag ccgttgacac gccaggtgtt aatcctgtta atgctattga
     7381 ccaactggca agtgttccta catcgccacc ggcgtcatct ctaatttgta agcgccataa
     7441 gccatttagg agcgtaccat taatattatt gtaattacca gaccagtcgt aaattcctgt
     7501 gttaatattt ccaccgcttg ctacgcctgc ttctaataaa gcggtgccac ttattgcaaa
     7561 acaatattgt ccattcaggt cgttgccatt agctcctgag ccgggtgtca attgtaaaat
     7621 gccaccattc ggtgccacta attgtgctac tacgtctgaa gcataggtgt gttctggatt
     7681 aaatgttaaa caaacattta tattggtatt tgctgtcaat gttgcaccag cagtaaatcc
     7741 agaaacatta atatctctat tgctccaaga agcgctattg tcaggtatcg cgagtgatga
     7801 agtatttgaa aaacttaaag tgttgctact tggagcagaa ggtaatcctg gcaaaccgcc
     7861 agtcggcgaa gtaagggtca gtgaaattcc attacttcca tcgccaggtg cagcacaagt
     7921 aggtgttatt gttggcgttg taacacgttc taatagcgta acttttgtag gcggtccata
     7981 gtcataacaa gtagggtcat tagttaataa atctgtgtta gggtcgtaat ctatcatggt
     8041 aacaggcgtt agccagaatt cttgaggacc atacttaaaa gggtcattgt tgacaatagt
     8101 taaatcgcca tgtcgaggtc ctatgcttgg tggcccaatc gatgtttgcg tatgtactaa
     8161 atcaattgtc caaccaaaat accaaaagcc ttcgttatct aagtcatcgt catcgattcg
     8221 taatcgccaa gtgccattaa gtgctgtgcc aacaagtgga gcaaagctcg cttccggtct
     8281 aaaatcgcct gtaagctcat tgtttaatgc accaaattgt ggtacaccac cgatggctaa
     8341 acaagaagag ccaattgttg tcggtgcagt tgaagtaaag catacactca taccaccaga
     8401 tgattcatta cagatatcag agaataaatc tatagttgca cctgatggac ttactaattg
     8461 gacatcgtgt gagccaatgt cttcaaatcc gaaataaacg catactttaa tgttatttac
     8521 attttgtaag gttgctccag cagggaagcc tgaaatggca atatctttat aaataggcgt
     8581 gtcccagtag ccataactaa tatcgccata tccaccagca gggctcctga caatattttc
     8641 tttataaact tttgttgagt ctgtatatgc agtacctggt gtaataatgt ctgtttgacc
     8701 aaccaaatat gtcaatgaag tattagatgg atcaaaagga tctaaagcag cacctgttgg
     8761 ttcagttggc gaaaataacc atgcaacaac aggattgtct tctgcatttg aaccaaaatt
     8821 aattggatca gcagttacac caagcgtcat gcgttcgcca tagcacaaaa cagtatctga
     8881 tttttcgaca gcacctaaat ctgctgaaca gcaaagatta ctattaattt taaattcaaa
     8941 atcacattgg aaacccaaaa agccatctat taaaatataa tatttctgcc caccagtaag
     9001 attgagtgtt acttgtccgt ttaaagcacc agtgcttatt gcacttgttg gaagctcaga
     9061 aaatgttcca ttgcaattat tagtggaact agtatataat gcaaactgaa tgccagcacc
     9121 atttgccgaa ctaggaaatg ggaaaagaga attttgtata tgccctaaac agtcataacc
     9181 ttttgcattg gcataggaaa ttgtataact gccagtaact ggcggcacat agtagtacca
     9241 aacagagttt tcagttgatc ctggcgtgaa gctaggatcg ccattacaaa gtgatgttgc
     9301 atttttatta gatgcaggga ttttaccaca ggcaatattg gtgatatcta ttgcaccact
     9361 acaattatca tttgaaggtc gtgttggtcc accacctgca tcaactacac gaatactaaa
     9421 attaccacgt ctttcagaag ctgtcggact gtcatatcca tcaatcatga tatagtttgt
     9481 ttcgcctggg aataatcctc tcagctgtaa ggtttcattg gcatttacac caccagcatc
     9541 tgcacaaaaa ccaccatggt caacaatatt acaatctgta ttgaactcag tacaactaga
     9601 acaaacatct gaagttaatt gacctatgga acaatcaaaa acggacgctc ttctgctact
     9661 caaccaataa atttgagcat ctaaattatt agatgtacca acagctgtga gattgatgtc
     9721 agcatttcca gatggaggcg tgataaacga aaaccatacg ccatagcctg cagaatttgc
     9781 tgtgcacgaa acttgttcat ttgcattata cactgcagat atgttgtcag aagctaatgc
     9841 tggtgcacca acagttaatg gccttgcaat acaaggtgca tcagaagttg gtcctggaca
     9901 atatattggt gtttgaatgc tcaatgtatt acatgcagca ggtgagccag tattgtatgg
     9961 tggattccaa gcaacattaa cagcagtgaa gttgccacca acggtaccaa cagcaactga
    10021 ttgacttaaa gcacctcctg tgacacttaa tgctgccggg caacttgggt taatagttac
    10081 aacttgtgag caaccattgg cagtataagt aatatagtta cttaatcgtg gatagatatt
    10141 aaatgtacat gcactcgttg ctcttgtgca gtttgttgtt gcaggttgag gtagtggata
    10201 agaaggaaat cctggaatag tagaagcacc gttgtcagct aaaatataac cgataaaagt
    10261 ataggttgct ggcgtacaac ctgggtcagg taagcctact gaaggattgt aaatagagta
    10321 ggtgtttgga tcatctaaat tgatggtgtt gcaaggtgta ccaaatgtat tggatttgca
    10381 atatgcccat ctggttccag gattactacc ataataggta acgcccatgc ttgcgctcaa
    10441 agcacttcca gcgcaggaag aagcaggatt gcaggtttgt gaacttacga tcggtcgttt
    10501 tacttcaata ttgaaattac attgtgcacc tgcattgcca tctataacaa aataatatgt
    10561 ttgtcctgct attggtgtga atgtaacaga ggtagtgcct tggctaattg gagttaatgt
    10621 ggcgcatgaa ccgctaaaaa taccaaactg tatgcctgta cttccatcca ttccaccact
    10681 acagcttatg cctgttacgg ttaaagtcac ttcactattt ccattattgg ctggtgctgt
    10741 ccatctatac caaacgtcat tttctagcgt taatgcatcg acatattcag gatcagggtc
    10801 tgtgcaacct gctgtagcac caatggttgt gcctgcttgc gctacacata aatttaagtt
    10861 agtagcattt gtacataaat catttgaagg aattgcatct ggcaagccac tattattagt
    10921 taattctggt ccaggttgcg gaatagcact tgatgtgagc gtttcgctta cttgtatggt
    10981 aaagtttgct gttggataag agaatgtgcc actgacccaa acataatagg tttgccctgg
    11041 aataacgaaa gcaaaaaggc gctcaacgcc gtctcttcct gcataatcat tacaatcgct
    11101 gacaacattg ttttctccta attgtgagca acttaaatta cagtcattta taccttcatc
    11161 aagtccagcc attggaccaa tgatattaat ttttacatct gaagcagcat tagggtcaac
    11221 atttatttgt actctgccag aagctggtgc aacgaagcta taatatacat cttgatagcc
    11281 tgtacagcct gcatctaaat cagatgatgc gccagcattt gtaccattga taggtgctgc
    11341 gttaacagtt aatgggcttg cattgcagat gacgtcattt gctacttcag tccagtaggg
    11401 caagcatacc cgacttaacc agcagtctgc tccacttact gtaattgttt tacaaacttt
    11461 atatgttgaa agattattta agccattgta aataccaggt gaacttggcg tcaaaggtgc
    11521 aacggcacag ttgtcccaaa cttggtagct aatgtttgcc gagtttaaac atgtcgttgt
    11581 gtttgcacca ttggttgttg taatttcata gcctctattt gtaggaaact gaacagtagt
    11641 ataaggcgca ggcacattga aagtgccaca aacagtcgat gtttttacgg atgcataagc
    11701 aaaattggag tgacaggcac catttgctgc agtttcatcg aagctataat cgtcaggact
    11761 tgcaaagtaa tatgttttgc agtctgcatc attacatgtt gtacattgat taacaccaga
    11821 acaagaaaat gtagctgttg tgcctgttcc tgagccaggt gctccaccaa agaaatctaa
    11881 ataaaatcct tcaggtgtgc catcataatt atcgattaat aaatacagta cttgacctgc
    11941 agtcactgtc atgttgttag agtataaatt gcctgatgca ccattggaat ttccatttga
    12001 tgtgcctctt ataccagttg gagatgcagg tgctgcaaaa ttacatctgt ctggagtgcc
    12061 taaagtgcca ccacttggac atgtgccacc tacacctctg ttccataatg aaaagtcata
    12121 atcagcagag ccgttatatg gacgtatcgt aaactctaag gttccaccag tttgaatgac
    12181 aattcgaaac caacgtgtgt ttttttcacc tgctaacata caaccacagc caccagatgg
    12241 acattcttgt gtgcctacac catcgtttaa ttgctctgct aattgattat tacagactgt
    12301 aattgcatta gcacaattat tattttgcgc ttctgctttg cttgcaataa ataataataa
    12361 acaaagcaat agaaattgta aaaatttctt cataatcact tgtttgtttt ttctattaaa
    12421 tctctaattt cttcgagttt tcttgtgttt ttttcaagct cataattcgg attgttcgtg
    12481 ttaagttcaa tttctctttg caaatcattt gcttcttgta ataaatagtc ttttttattt
    12541 ggattaacag gtgctgaatt ataatctacg attggatgga tatctgcttt tacttgttta
    12601 ggcacatagt ttatggaagg ttttattttt gtatagcttc gttcagtgtt tgtcgtcttt
    12661 tcattttctg aaaatgttac tttagtatag ctagcatttt cagttgctgc agtattgttt
    12721 tgaatagaaa tttctagatt atttatgcct ttctctgttt gaattttttg tatgcttaat
    12781 gaatcattta ttctaagttg cgtggttgtg ttgtttgtat ttatgccttt tagattttct
    12841 tggatttcag ctttgtttat gcccaacacc tctgtgttgc tttttgtttt tatattgttt
    12901 tcggtgtttg tttttttgaa attatttatc ctattttctt gtgctatgat tgaaaaataa
    12961 ttacaagaaa aaaagaatat aatcaacaaa atgtgtaaaa ttttatacat agaagttggt
    13021 tttactttga gagtactttg ttaataaagc ataatattac aaatttattg acattaatca
    13081 atatttttta agtgttattt tttataatag ctgttttttt attcattttt ctaaatcaat
    13141 cctactactt ttgcgcaatg aaagaacgtg tattaagtgg cgtgcgtagc accggaaatc
    13201 tacatttggg caattatttt ggagcgataa agaattttat ttacatgcag gataactatg
    13261 aagcctattt ttttatcgct aatttgcacg cattaacgac gcatccaaat ccagaattat
    13321 taaaaaagtc agtgcgcgcc accttggtag agtacattgc ttgcggcctt gaccctgaaa
    13381 aaagcaccat ttttgtgcaa agtgacattc ctgaaatttc agaattatat gctttactaa
    13441 atatgttagc ctataaaggt gaattagaaa aatgtacgtc atttaaggaa aaaataaaaa
    13501 aacaccaaga aaatataaac gcaggtttgc ttacctatcc tgttttaatg gctgcagata
    13561 ttttgattca tcgtgcacac aaagtgccag taggcaaaga tcaagagcag catcttgaga
    13621 tgactcgtaa ttttgcagta cgattcaatc atttgtttgg tgttgatttc tttccagaac
    13681 cacaagcata tagcactagc ggtaaattag tgaaagtgcc aggcttagac ggaactggaa
    13741 aaatgggaaa aagtgaagga gaaggcaatg ctattttttt gagagaagat gcagaaagca
    13801 ttcgtaaaaa ggtaatgcga gcaaaaactg atgcaggccc aacacaagcc aatcaaacaa
    13861 aatcagaaga aatacaaaac ttgtttactt tgttagattt agtgtcaaca cctgatactg
    13921 tttcctattt tgaagataag tataacgact gcactataca gtatggatat ttaaaaaaac
    13981 agttggcaga agatattatt aatttctgtg cacctttgaa agaaaaaata atcgcgcttg
    14041 aaaatgacca tgcttttatt aataaaatag ccaaagatgg tgcagaaaaa gctcgagtaa
    14101 gtgcacgtgc aaccttagat ggcgtacagg aaattatggg tctaaagcct atttggaagt
    14161 aagctcaaac atttatttac atcactttga aaaatgcact agcaatcatt caaccgttct
    14221 gttaaaaatt tttactttct taaaagataa aacttttcca cttttttgaa aaaaattacg
    14281 acagcttatt attcaatgaa aaaaaatgta tagcttagtt gtccttaatt tacactaata
    14341 aagcgttctc gtggcaaaac aaaaaaaaca attaaaacat attgctgttg ctggaaatat
    14401 tggctccgga aaaacgacat tgactgaaaa attagcaaaa cattataatt gggaagcgcg
    14461 ttttgaagat gtagaaaata atccatatct aaatgacttt taccaagaca tgccacgttg
    14521 gtcattcaat ttacagattt atttcttaaa tagtcgttta cgtcaattag tagaaattca
    14581 agaaggcaca caaacagtta ttcaagatag aacaatctac gaagacgcac atatctttgc
    14641 accgaaccta cacgaaatgg gtttgatgag tacgcgtgat ttcgaaaact atattagttt
    14701 ttttagaacc attagcaaga aggtaaatgc gcctgatttg cttatttatt taaaagcatc
    14761 gctaggaact ttagtcggac aaattgaacg cagaggccgt gcctatgaag ataatatcag
    14821 attggattat ttaaaacaac tcaaccagta ctacgataac tggatagaaa attacaaaga
    14881 aggaccgctt ttagtgatta atgtagatga tttaaactac gcagataaga atgctgattt
    14941 tggcaaagta gtagaaaaaa ttgatgctga gttgttcgga ttgttttaaa aatatttcta
    15001 tcattttaaa acaaaatcct tagttaattt ttttgtgtat taatattgtt tccattatat
    15061 ttgacacaaa gacgcatcat attttttctt tgagtgattg tcaaatacta caggaaaaaa
    15121 agatacattt tatcaaacta tatgccttct tttagaaggc tttttttata actaattaat
    15181 atttttttat cagtaattac aaatttgaga ccctacaatt acatgcagga caagaagctg
    15241 accctacaac aggcgcgaga gccgtgccaa tttatcaaac tacatcctac caatttaagg
    15301 acacagaaca cgcagcaaat cttttcggtt tgaaagaatt tggaaatata tacacgcgta
    15361 tcatgaatcc aacaacagat gtttttgaaa agcgtattgc agctttagaa ggaggtgcag
    15421 cggcattggc aacagcttct ggccagtcag cacagttttt agcattaaac aatatattac
    15481 aagcaggtga taattttatt tccacgccat ttttatatgg aggtacttat aatcaattta
    15541 aagtggcttt caaaagattg ggcatcgatg cgcgttttgc tgagggagat aatcctgcat
    15601 catttgagaa attaatcgac gataagacaa aagcgattta tcttgaatca attggaaatc
    15661 caagaggcaa tgtgcctgat tgggcagcat ttaaagcact ttccgacaaa tatgatattc
    15721 caattgtagt tgataataca ttcggtgctg ctggttattt gttccaacct tttaaacatg
    15781 gtgcacatgt tgtggttgct tctgcgacaa aatggattgg cggacatgga actagtattg
    15841 gtggcgtaat tgtagatggt ggcaattata attggggaaa tgggaaattt ccacaattta
    15901 cagaaccatc agaaggctac cacggtatgg tgttttggga tgtgtttggt gacaaaggtc
    15961 catttggcaa tattgcatat attatacgtg cacgagtgga aggcttgcgt gattttggca
    16021 atgccatttc tccatttaac tcatttttgt tattacaagg tgttgagacg ctgtctctac
    16081 gcatggaaag acatcagcaa aatgcaatgg cattagctca atggttaaaa gaacatccaa
    16141 aagtggcaag tgttacctat acaggattgc cagattctcc ttatcatgaa ttagcaaaaa
    16201 aatatcttac caacggattc ggttctttat ttactttcga agtaaaaggt ggttatgatg
    16261 ctgcgaaaaa atttataaat tcattgcaat taacttcaca tgttgccaac ctcggcgatg
    16321 cgaaaacatt ggcaatacac tctgcatcga cgacgcatga gcagttgaca gacgaagaac
    16381 aaatagccgc tggagtttca aaaagttcca ttcgcgtttc tgttggtata gaacatatcg
    16441 acgatataaa ggctgatttt gatcaagcat tgaatgctta attataaaaa ataagacaca
    16501 ctttataaag aggtacagac aatttaaaat ggctgtacct ttttttcttt atcttcgtaa
    16561 taatttttaa aaatacaata ctaataaatt tatgatagta gataatgcat tagtggataa
    16621 attagccaat ctttcgaagt tagaatttga tgcagaaagc aaggaagcca taaaacaaga
    16681 cttgtccaaa attctgaatt ttatggagaa gttaaatgag ttaaagacag atggtgtaga
    16741 gccattaatt tatataaatg aggagccaaa tgccagtcgt gctgatgaag taaactatcc
    16801 tattaccaaa gcagaagcac tgatgaatgc acctttacac aatgaagatt ttattaaagt
    16861 gcctaaattt gtaaaaggaa caaaatatta atcatacaga ttattataaa ataatacgcc
    16921 aaagcaattc cattatattt atgtcaaacg cagtcatcaa cttaatagat attaaaagag
    16981 cgtttcatct tggagatgaa gtgattcatg cgctaaaagg cattaattta agtattaata
    17041 ctaatgaata cgtggcactc atggggcctt caggttctgg aaaatctact ttaatgaatt
    17101 tattaggctg cttagataca ccatcggcag gtcagtattt actagatgga atagatgtca
    17161 gcaagtcaac agataatgaa ttggcagctg ttcgcaataa acaaattgga tttattttcc
    17221 agacatttaa tttaattccg cgcttgacaa ccatagaaaa tgtagcttta ccacttatat
    17281 atgcaggttg gaataaaagt gatcgagagg cacgtgctat cgaagtcttg catcaagtag
    17341 gcttaggcga tagaatgaag cataaaccga atgaattgtc aggcggtcag cgtcagcgtg
    17401 tagccgttgc gcgtgcatta gtaaatcgac catcgctaat acttgccgat gagccaaccg
    17461 gaaatttaga tactaaaact tcatacgaaa tcatgcgcct gtttgaagaa atacatgcca
    17521 atggaaatac aatcgtcgtg gttacacatg aagaagatat agccatgtat gcacatagaa
    17581 ttgtgcgtat gcgcgatggc ttgatagaaa gtgacaagct aaatgaaaca ataaaaaaag
    17641 ctactgcagc attataatca tttaaaccat acgagagcgg cctgtttctg tccgtaaatg
    17701 ctacttagaa atgaaacaaa atagctaagt tttttgagaa cagtttaatg gaaatcgata
    17761 ataagataac accgaatatc tttctagaaa tatttatgcc agtatcgcca agtagatgtt
    17821 caatttttgg aataaatcga atcacaatat aaataaatgc aagattgata agaattgcaa
    17881 tcattatgac ttgcacatga tattcagacc gaagtgacaa taaggtggta agcgtaccag
    17941 caccagcaat cataggaaaa gcaataggca caacggaagt gctttttggt gtcgagtttc
    18001 ctgatttaaa aatatcgcga tttaacgtca tctctaagcc tatcaaaaat atcacaattg
    18061 agccagctaa agaaaacgaa gccacatcta tgcctatgag gttcagcaat tctttgccta
    18121 aaaataaaaa aacaatcatt atgccaccag agacgcctgt tgcatacttg gcatcaactt
    18181 gccctacttt agatttaata tctaagatga caggcacaga tcctaaaata tcgatgatgg
    18241 caaacaatat taaggtaaca gtagatagtt cttttaaatc tatcggcata gcaatttttt
    18301 cacaaaggtc tgtattttat cgcacttcaa caagcgcgta taggtttcac ttttttagga
    18361 aaataatgta tcgtaatttc ttttaaggac ttgtttttat acttaatata gaacacatta
    18421 taatcagtta tcaacaatca gttacacaaa atcaacgatt ttgtaataat ttactaaaaa
    18481 agttgtttaa atttgtgaat taatataaat taacactatt tttgaccaat aaattaaact
    18541 aaagtcttta tgaaaaaagg tatcgtatta ttatgtgcac tgcttgtatt agctagctca
    18601 gtatctttag gtgctaaaaa gccacctaag agtaataaaa gtgcagtagg aaacaacaga
    18661 tttagcttag gcatcggcat ggaagtagca gattatattt cacctgccaa caaaaaggta
    18721 acaactttca aaacgccagt gtcttttggg ccacgcttta cattttggta taatcccaat
    18781 tcatcagttg cagttggtgc tgacttaggt agccatgcat tttcttcaaa aacagatgca
    18841 agtgctactg ctattaacac atataatgta ttatatgcgg cagtggttgc gtataaattt
    18901 aataacggtt acattttaaa agaagacgct gcggtatctc cttacttatt tgcaaaatta
    18961 caaggctctt gggctgaaac accactttac aaagagtcag ttcacggttt tggtattcca
    19021 atcggagcag gtattaattt caaagttgca aac
//
LOCUS       DAOQQV010000043        18826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_892_length_18826_cov_7.575729, whole genome shotgun sequence.
ACCESSION   DAOQQV010000043 DAOQQV010000000
VERSION     DAOQQV010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18826
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_18826_cov_7.575729"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="PLL59_06105"
     CDS             <1..1667
                     /locus_tag="PLL59_06105"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HMY23414.1"
                     /translation="IKGSKYRAVSNLFGTTSRSEFIFRDTLPAVKKMIALKSDPMQAL
                     KNPIQNIGVALAAWKALPKKVSSHSFEKIKISDLPQIHCWEKDGGAFVTLPIVYTEDA
                     DKPGAMHSNLGMYRIQLSGNDYVQDKEIGLHYQLHRGIGVHQTKWNKKGMPMKVSVFI
                     GGPPSHSFAAVMPLPEGLSELTFAGALGGRRFRYMYDEEGYCLSTDADFVITGEVYPN
                     ENKAEGPFGDHLGYYSLKHDFPLMRVKNVYAKKNAIWSFTVVGRPPQEDTAFGNMIHE
                     MTGTAISKEIPGLHEVHAVDAAGVHPLLLAIGSERYTPYYKEKRPQEILTIANHILGT
                     GQLSLAKFLFICDKNDNETLSTHDEEKYFHHILERIDLSRDIHFQTKTSIDTLDYSGD
                     GLNTGSKVIIAACGDKKRDLLDKLPTDFSMPSAFKNRRMVTNGILALEMSNAQWSMLN
                     SNDIADLNKMWSLHKAYFEKIPLLIICDDADFVTANFSNFLWVTFTRSNPSHDIYGIN
                     ERFENKHWCCDTLIIDARIKSHHAPVLEKNAAAEKRVDEILSNGKF"
     gene            complement(1664..3304)
                     /locus_tag="PLL59_06110"
     CDS             complement(1664..3304)
                     /locus_tag="PLL59_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMY23415.1"
                     /translation="MKNSLLLFCSLITLSAISQSLQRGPYLQSMTNNSVKIMWRTSDS
                     TTAIVKYGTSPTTLTSTITDNTLTKNHIVQVNGLQAKTKYYYSVGYNTTVLAGENDQH
                     HFITAPIPGDSSEFKFWVTGDFGGGNGGQIKVRRWFENYLQGNNVTGWLWLGDNAYND
                     GTDAQYQAKVFDTYYGYDSIFRFLPFYPIPGNHDYNSVNGADDPITHKGPYYDMVEVF
                     KNAEMGGVPSGMEAYYSYDYGNTHFLALNSELYRVFVLWDLHASAIAFKNWLINDLKS
                     SDKKFKVAYWHQPPYSKGSHDSDDFWEVFMGSMRDKVIPILEQYGVDLILCGHSHVYE
                     RSYLINKHYGLSHTFNRSTMLIDSTSGNPDSNRTYLKYTYGANKDKGTVYGVIGNSGK
                     SEPENGKMHPAMYKKYAADGGVGSMILEVKGNQMTCFYYKENGELYDKFRILKQDSTA
                     IISGIRNNTSVSDLKIYPNPFNNAIVVEFDAKAIKTTAIAIQNIIGQLIVETIWTGKS
                     NLGNNRIEINNLQGLPSGEYIITIKQDDEVVSEKIVKF"
     gene            complement(3374..3835)
                     /locus_tag="PLL59_06115"
     CDS             complement(3374..3835)
                     /locus_tag="PLL59_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HMY23416.1"
                     /translation="MKTQSIFIFLIVLFILSACEQKHVYNKYESVDTKAWKATDTLSF
                     PINIDTENKSYQYAISVRYSNDYEFSNIWLKVSIKGNQIDTSFRYEIPLFKNDGKPYG
                     ENSGSLFTQTIPLKTNLPLYQKGDYTFTVVQLMRKDPLNGISDIGIIVDKK"
     gene            complement(3891..6041)
                     /gene="ligA"
                     /locus_tag="PLL59_06120"
     CDS             complement(3891..6041)
                     /gene="ligA"
                     /locus_tag="PLL59_06120"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HMY23417.1"
                     /translation="MSNDLFSDVNSIDSRISLTASLLNKIDDFVTEQESEQFCDDIKT
                     VINFHDNQYYVKSNSLISDYDYDRLFKKLKELEENFPNLVTPDSPTQRIAHGLNNDFP
                     TVQHLVPMMSLNNSYNITDLEDFDTQVKKALTQQAAVEYIAEPKFDGSSIALIYQDDR
                     LVRAATRGNGVAGDEITLNAKAIKTIPLKANFSEYGIAKIEVRGEVLLELAMLNKLNE
                     DRKKQNEQLRAENKKELDLYKNARNTAAGSLRLKDSSEVAARKLDAIIYQIGYAENAA
                     GIDITDIFQTHNKNLEILEKLGFKTPFHEKKIFTSILDVETFCKYWENKRDTYHFDID
                     GMVIKVNSIHQQQLIGKTSHHPKWAVAYKFKAKQAFSKLLNVEFQIGRTGAITPVAKI
                     EPVQLMGVEISSISLHNEDFILDKNIMLNDTVIVERAGDVIPYIVGSVPEKRNGAEIK
                     IQFPTTCPSCQHALVKPLEESVWRCINTNCPAQLEEHLIHFVSKQAMDIFGFGEENVR
                     TFVANNIIQNITSIYTIDYDKVRQLEGWKEKSIQNLKDGIEASKHNELHRLIVGLGIR
                     HIGSTTAKLLAKKIEKLSDFQTWTQEQFTELEDIGPKVAQSLFQFFGDAENINTIETL
                     STLGVNIYKTETTLASEKLSGKTFLCTGTFPQHSRDDIKVMIEKNGGKNLSAVSANLD
                     FLIAGEKAGSKLAKAQKISSIQIIDEATFLKMIE"
     gene            complement(6038..6274)
                     /locus_tag="PLL59_06125"
     CDS             complement(6038..6274)
                     /locus_tag="PLL59_06125"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HMY23418.1"
                     /translation="MTTWNTKDNKLEKEFIFKDFVAAFAFLTKIAIVAEKQNHHPEIY
                     NVYNKVKISLQTHDAGNTVTEKDFTLAKTIDSLV"
     gene            6312..7235
                     /locus_tag="PLL59_06130"
     CDS             6312..7235
                     /locus_tag="PLL59_06130"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMY23419.1"
                     /translation="MNWKLRFLLWFVNNVQPLREVENKDIAKERRNTGLASNLGKLLF
                     DKKIKVYSVEDAVFKGVPVRIYRNSKQANQKVILYFHGGGFVFYNIESHDYVTRRLCA
                     MNDCIVISVDYRLAPEYSFPAAHEDAYKVISYVHEHAENLLIDNNKIIVAGDSAGGNL
                     AACAAHHFKKNKEINIAAQVLIYPWIDGKIASDSIEKYQEGYLLTKKAMYWFQQQYTP
                     KLEDRLNPEVSPIYHKDFKQLPPAFVLTAEYDPLKDEGKFYADTLKENGNVTIYKDYK
                     HLVHGFFNLPKLSEESMQAYYDIQAFLRKVV"
     gene            complement(7236..7631)
                     /locus_tag="PLL59_06135"
     CDS             complement(7236..7631)
                     /locus_tag="PLL59_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMY23420.1"
                     /translation="MNIKRRRPFHDEDAMASMNDIMFFLMLFFLIISTMANPNVIKLM
                     LPKANTTEKLPKQPVTLSVTADKHYFINTTEIPAEGIENALSAALENNPDPTVVLRVA
                     ENLSIQDLVDIMSIGAKLKLKMVLSTDKK"
     gene            complement(7684..8391)
                     /locus_tag="PLL59_06140"
     CDS             complement(7684..8391)
                     /locus_tag="PLL59_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMY23421.1"
                     /translation="MGILLQAAQVASTVVDTANAIMPAAAPPVQELSVLGLLTKGGVI
                     MIPLVAFLAIALFFIIERYLFFSQRSKINDNLVREVLDKLYAGNMQGADALCTADKSA
                     LGSVLQAGISQLGKPIDHIEKALDIQSNIELNEMEKRMSWISMISGVAPRLGFIGTIL
                     GVIKIFYNISQSADISIGTISAGLYEKMITSAAGLVVGVIAFMGYHFLLTKIDNYSMK
                     LQKQIFEFVRGIQKPVK"
     gene            8590..9825
                     /locus_tag="PLL59_06145"
     CDS             8590..9825
                     /locus_tag="PLL59_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMY23422.1"
                     /translation="MPEYDLNKNYPGSQYKFSFVQLSDIHIGEGFGDYGTSGYFNDTI
                     PVVDTSDPANDLRAAIRWINENKMNKNLKFVVISGDLTGSAEKSEFQMCKNLLNTLEI
                     PYVPIIGNHDIWPYVRYQDEAPYACGDSVMNEIFDDVYTQNASFFSNWNDGSRLQRTY
                     NPETQREHYLQNFSFEYDNFIFYGLDFNPRYHVNKAEPGIGPEAQLMDWNGGTFQWLK
                     NELTNNPRKGNKNICFISHHPATDNILFILSGFVFDANEYIKMMDMLTPFKQHLGLWM
                     AGHIHIDYNYPLVNNIMPVIGIAANKDFDSAHFRIVNVYEIPTATAIRTNVNSSKLQV
                     FPNPSNGLFHVSGIALSNTATIQIFDVLGQSVYSKNRNEMNMQENEYSIDISKFPKGV
                     YLLHVNDGASEYFQQLILH"
     gene            complement(9826..10755)
                     /locus_tag="PLL59_06150"
     CDS             complement(9826..10755)
                     /locus_tag="PLL59_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23423.1"
                     /translation="MKKAIVLLLSASVGFLFIISSITKIYPMEPFEFQFVDIGIANWK
                     TAPYLARVFISLEFFLGILLVLNIAIRKFTIKFAIALLSVFSFYLLYKIISVGNTGNC
                     GCFGEYFKMSPLEGIIKNIVLIISCVLCYLLSDFSIWKNQHLKFVVPLLLLISLCIGF
                     FLYPINIQYSSSIDTARVNYKVPLDLMYSATQKERPNIELRQGKHIVAFLSLTCSHCR
                     VAAQKLAIIHKRNPALPIYFALNGNKKKLPTFFDETHSKAIPHNLFLGPENWIKVAGV
                     SLPIVMYLENSIVVKKCILPEIDQEDMEVWMAK"
     gene            complement(10813..11025)
                     /locus_tag="PLL59_06155"
     CDS             complement(10813..11025)
                     /locus_tag="PLL59_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23424.1"
                     /translation="MKNKILIAVALICIFATSCVKDWKCECTDGTTTQVVETYPSTKM
                     LDAKKVCDQRESDIKKINSSVTCKIK"
     gene            11146..11610
                     /locus_tag="PLL59_06160"
     CDS             11146..11610
                     /locus_tag="PLL59_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23425.1"
                     /translation="MPVVQFNQVSEEEKELLYNATALVTYLIGGADNNFDEKERAQSA
                     HLVNIRTEQGDPMLFDYYLDLQVDFAERVAKVEAKYGVDSVNDRTALLVAELEKLNDI
                     LPKLDDIYARAYLKALRTLAKGVAEASGGLLGFLEISYEEEHLMGLTMITFE"
     gene            11613..12113
                     /locus_tag="PLL59_06165"
     CDS             11613..12113
                     /locus_tag="PLL59_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HMY23426.1"
                     /translation="MPIDHQINGKPIPESLQKIAKITKLMDSQFRIPGTKFTFGIDPI
                     IGLIPGLGDLIDYVISAYLLIAMVRNGASGRAVAKMIINITIDGIVGLIPFFGHIFDV
                     FYKANRRNLVLAVEHFEEGKHQGSAWPIVLPILSVLLLVFILFAVLTFYVVKYMLIFL
                     FPTGGI"
     gene            complement(12149..13216)
                     /locus_tag="PLL59_06170"
     CDS             complement(12149..13216)
                     /locus_tag="PLL59_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23427.1"
                     /translation="MILKLINNLLSVGKNTKKAEPKKTSVAAKPVAKKTLASKETKSV
                     SKAKISAKPTAKKAAPTPVAKKVTPVAPAKKATPKKVEEKKSNVKKAAPKVAPKKVAP
                     APAVKSKSPVKNTKPSNAVTKQTVSAKTTTKKAEPIKKNEETKLVKKVTTPVEAPKKV
                     AVATKTVEQVSTKKEEPKKSKLEEKAPAVKIAPTPPPGGFKKVIVDYKNVPDEVLKAL
                     YEKYPHGYNKGIIRFTNAKKELVSAVPIELGEYSYLVKVSTQLQKMVDDYDEDELFDN
                     VASSSEKEISVGAGGDFDDFDKSDDDDSPKRKAKGKVKSADDLEYGFSDDDDDDDDDD
                     DDDDDDDDDDDVSDDDDDDDE"
     gene            13326..13655
                     /locus_tag="PLL59_06175"
     CDS             13326..13655
                     /locus_tag="PLL59_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23428.1"
                     /translation="MKKLLILIVISLVMLHSQARPKVYVKVNKNEHRYVKVCPSATNV
                     VVVKPACPSPKHIWIDGDWIWNPQINQYVYVQGKWIIPISTKSIWIAGHWKNSRYGWH
                     WVPGYWK"
     gene            13825..14826
                     /locus_tag="PLL59_06180"
     CDS             13825..14826
                     /locus_tag="PLL59_06180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMY23429.1"
                     /translation="MIETDICIIGAGPVGLFTVFEAGLLKLRCHLVDYLPQPGGQLSE
                     IYPKKPIYDIPGFPTVDAQELVDNLLEQGKPFNPTFTLGERIETINKLEDGSFELFTH
                     LDTKIHAKVICIAGGLGVFEPRKPAIENLTAYEKNGVEYMVLDPEKFRNKKIVIAGGG
                     DSALDWTLFLADVAAELTMVHRGETFRGAPDSVDKVKQLAAEGKINLALSSNITHLHG
                     DGKLEKVTITHNDTSTKEVEADNYLALFGLSPKLGPIANWGLNIEKNAINVDTRDYST
                     NIKGIYAIGDINNYEGKLKLILCGFHEAAIMCHSAAELISGKKPTLKYTTVNGVNSF"
     gene            14880..15209
                     /locus_tag="PLL59_06185"
     CDS             14880..15209
                     /locus_tag="PLL59_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMY23430.1"
                     /translation="MDAVTIHIQQADFSFQALEIPLGVDLSLMEILKGEEHPIEAICG
                     GMAICGTCRVEVLNKDEIMESGPNDDELCMLETLPCYTGNCRLSCQIKISEKIDGLKI
                     RIPVENS"
     gene            15294..18371
                     /locus_tag="PLL59_06190"
     CDS             15294..18371
                     /locus_tag="PLL59_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY23431.1"
                     /translation="MQFYLISIVDICYSFALEKIEPAAKIDIMKYACNIILLIILSSL
                     SLNVLAQHQKDTTKLIDKLKDIPQVVVRKSNKEFTIKGNITDKLTKEPLIGVNIFVET
                     DKVRGTVTDFDGNYELKANEGDVIVFTYMGLRDERYTVTQSQTYNLIMEEEGNVMDEV
                     VISVGRKKEKALDAPASIVSVDATAIKNKAGLGITDHIKNSAGVQITSTGIQGGQPSV
                     RGFAGYFNSDVMSLTDTRVAKLPNTGLNMYQMMTTTDADIERIEVLKGPAAALYGPNA
                     NQGVIHIITKSPIKYQETKFWTSIGARVKIKDTLQDINRENPRFDYDKIAKRMIFSLG
                     FMVSDTIHSKNPNVKMGMKVSSQFFTGHDWRYSDVGDPAYVIRFKAGRDTIYNERPDE
                     TTDPSGKGVEVANERDEKILNGQVDARYDIQIKEDFNIVLAGGVNVSNGIIPSPVGAM
                     QNKNWAYSWAQVRFNWKNLFVQAYMNGNNSHKTYFVPVGGRYIDKSHMYSVQAQYSHD
                     VFKKRINLIYGFDAFFTRPNTGGTLNGKYEDKDAIDEYGIYMQGDYRLHPRFNIIVAT
                     RLDYNTAVKKVLFSPKVAIVYKPGTGHNLRFTFNRAFKNPASAAFYVDAKQGEIPGGI
                     QVRNVGTPRSGFNYSFNANPNFDGAMLPQFLSPFGTSGEFHDVGDKSFNTTGWDGILS
                     AIRGQFGGQAGIDDNSPILPIINLVVNSIIPQTIPGDINHVVKNLNTAKQVFESNNDR
                     WKKTEDIKPLKPTTTYTYELGYKGVIGKMFGMQIDVYRTDYKDYLAPVTLLTPAVMFD
                     DEQLMDYLGPRILQNYNNLNPVIQLAMNAFLDQSKKFNGNNNGTASDEFVSLIRRAVQ
                     NLPIGVITPKEAEGPNMLLVTRNIGDVVVYGVDFNSTLYLPKGISILANYSFVDKDSV
                     KVESAPLGYVALNSPKHRVNAGVNYNAEKIGFNIGLRFKWSAGFPVLSGNFIGRVKPT
                     HDMDLDISYTPKFMKDHFNMTVSISNLYNRKQQYFIGAPVMGLMGVCKLTYTL"
     gene            18448..18804
                     /gene="queD"
                     /locus_tag="PLL59_06195"
     CDS             18448..18804
                     /gene="queD"
                     /locus_tag="PLL59_06195"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HMY23432.1"
                     /translation="MQIFKQFSFDSAHFLPNVPVGHKCKEIHGHTYRLTVYIEGELDN
                     ELGWVIDFADLKHVIEPVINSIDHKLLNEIPGLDNPTCERIAIWIWNQIKPNVPLLSK
                     IELYETLSSGVVYEGK"
BASE COUNT         6018 a         3096 c         3378 g         6334 t
ORIGIN      
        1 aaataaaagg aagtaaatat cgcgcagttt ctaacttatt tggcacgaca tcacgcagtg
       61 aatttatttt tagagatacc ttgccagcag taaaaaaaat gattgcatta aaatcagatc
      121 caatgcaggc attaaaaaat ccaatccaaa atattggtgt tgcattggca gcttggaaag
      181 cattacccaa aaaagtttca tcacattcat ttgaaaaaat aaaaatatca gatttaccgc
      241 agattcattg ctgggaaaaa gatggcggtg cttttgtgac cttaccaata gtgtacacag
      301 aagatgccga caaaccaggc gctatgcatt caaatcttgg catgtaccgt attcaattga
      361 gtggaaatga ttatgtgcaa gacaaagaaa ttggtttaca ttatcagcta catcgaggca
      421 ttggcgtgca ccaaacaaaa tggaataaga aaggaatgcc gatgaaagta tctgtgttta
      481 ttggtggtcc gccctcgcat tcatttgctg cagtgatgcc attgccagaa ggtctatctg
      541 aattgacatt tgcaggtgca ttgggtggca gacgatttag atacatgtat gatgaagaag
      601 gatattgttt aagcaccgat gccgattttg tcattactgg cgaggtgtat cctaatgaaa
      661 ataaagcaga aggtcctttt ggcgaccatt taggttacta ctcattaaag catgattttc
      721 cattaatgcg tgtaaaaaat gtgtatgcta aaaaaaatgc gatttggagt tttactgtcg
      781 ttggcagacc accacaagaa gacactgcat ttggaaatat gatacatgaa atgacaggta
      841 cagccatcag caaagaaatt cctggcttac acgaagtgca tgccgtagat gcagcaggcg
      901 tgcatccatt attattggcg attggaagcg agcgatacac accttattac aaagaaaaaa
      961 gaccacaaga aatactcacg atagccaatc atattttagg aacaggtcaa ttgtcattag
     1021 cgaagttttt atttatttgt gataaaaatg ataatgagac attatcgaca cacgatgaag
     1081 aaaaatattt tcatcatatt cttgaacgca tagatttatc gcgcgatatt catttccaaa
     1141 caaaaacaag catcgataca ttagattata gtggtgatgg cttaaatact ggaagtaaag
     1201 tgattattgc tgcatgtggc gataaaaaaa gagatttatt agataagttg ccgactgatt
     1261 tttccatgcc atctgctttc aaaaatagga gaatggtaac gaatggaata ttagcattag
     1321 aaatgtcgaa tgcacaatgg tcgatgctta atagcaatga tatagctgat ttgaataaaa
     1381 tgtggtcttt gcataaagca tatttcgaaa aaattccatt gttgattatt tgcgatgatg
     1441 ctgattttgt tacagctaat tttagtaatt tcctttgggt gacatttaca cgtagcaatc
     1501 cttctcatga tatatatggc atcaatgagc gatttgaaaa taagcattgg tgttgtgata
     1561 ctttaattat cgatgcacgc atcaagtcac atcatgcacc agtattagaa aaaaatgctg
     1621 cggcagaaaa acgtgtcgat gaaatattgt cgaatggtaa gttttaaaat ttcacaattt
     1681 tttcgctcac cacttcatca tcttgtttta ttgtaatgat atattcgcca ctaggtaatc
     1741 cttgtagatt gttgatttca attctattat tgccgagatt acttttgcca gtccaaatag
     1801 tttcaacaat aagttgtcca ataatatttt gtatagcaat agctgttgtt ttaattgctt
     1861 tggcatcaaa ctcgacgacg attgcattgt taaatggatt tggataaatt ttcaaatcac
     1921 taacggaagt gttattgcga ataccagata taatggctgt tgaatcttgt tttaatatgc
     1981 ggaatttatc gtacaattca ccattttctt tgtaatagaa gcaagtcatt tgattgcctt
     2041 tgacttctaa aatcatactg ccaacacctc catctgcagc atatttttta tacatggcag
     2101 gatgcatttt tccattttct ggctctgatt ttccactgtt tccgataacg ccgtaaacgg
     2161 tacctttgtc tttgttagca ccatacgtgt atttcaaata agtacgattg ctatccggat
     2221 ttcctgaggt gctatcgatt agcatggtac ttctattaaa ggtatgagaa agaccataat
     2281 gtttattaat caaataagaa cgctcataga cgtggctgtg tccgcataaa attaaatcaa
     2341 caccatattg ctctaaaatt ggaattactt tatcgcgcat gctgcccata aatacttccc
     2401 aaaaatcatc agaatcgtgt gagcctttag aatagggtgg ttgatgccaa taagcaactt
     2461 taaatttttt atcagaagat tttaaatcgt taatcaacca atttttaaaa gcaatagcag
     2521 atgcatgcaa atcccataaa acaaaaacgc gatacaattc tgaattgagt gctaagaaat
     2581 gtgtatttcc ataatcataa gaataataag cttccatgcc tgaaggaacg ccgcccattt
     2641 cagcattttt aaacacttca accatgtcat aatacggtcc tttgtgcgtg atagggtcgt
     2701 cggcaccatt gacactattg taatcgtgat tgcctggaat tggatagaat ggcaagaacc
     2761 gaaaaataga atcgtagcca tagtaggtat caaatacttt tgcttgatat tgtgcatcag
     2821 tgccatcatt ataggcattg tcgccgagcc aaagccagcc agttacatta ttgccttgca
     2881 aataattttc aaaccaacga cgcactttaa tttgcccacc gtttccacca ccaaaatcgc
     2941 cagttaccca aaatttgaat tcagaagaat cgccaggaat gggtgctgtt ataaagtgat
     3001 gttgatcgtt ttcgcctgct aatacagtgg tgttgtagcc aacgctgtaa taatattttg
     3061 ttttagcttg caagccattg acttgtacaa tatgattttt agtaagtgta ttatctgtaa
     3121 ttgtactcgt aagtgttgtt ggcgaagtgc catattttac aatagctgta gtagaatcgc
     3181 tggtacgcca catgattttt acgctattgt tcgtcatgct ttgtaagtat ggaccgcgtt
     3241 gtaagctttg tgaaatggca gaaagtgtaa ttaaactaca gaatagtaaa agactatttt
     3301 tcattagatt ggttttaata aagttaagaa gataaatttt attaacctac gatagaaaat
     3361 gaaaattgcg tgtttatttt ttgtccacaa taatgccaat atctgaaatg ccgtttaaag
     3421 ggtctttgcg catcaactga acaactgtaa aagtataatc acctttttga tatagtggta
     3481 agtttgtttt taatggaatt gtttgtgtaa aaaggcttcc agaattttcg ccataaggtt
     3541 ttccatcgtt tttaaacaat ggaatttcat accggaagct tgtgtcaatc tggtttcctt
     3601 taatggacac tttcaaccag atattactaa attcatagtc attagagtaa cgaacgctaa
     3661 tcgcgtattg ataactttta ttttcagtat cgatattaat gggaaaagat aaagtgtctg
     3721 tggctttcca agctttcgta tctacagact catatttgtt atagacatgt ttttgttcac
     3781 atgctgaaag tatgaataat acaattagga aaataaaaat ggattgagtc ttcataagat
     3841 gaaatgtaaa ggtatgaaaa aataatgcat agttatattg ttcattctta tcattctatc
     3901 atttttaaga atgttgcttc gtcaatgatt tgaatactag atattttctg tgcttttgct
     3961 agtttgcttc ccgctttttc gccggcaatt aaaaagtcta agtttgcaga gactgcactc
     4021 aagttttttc caccattttt ttctatcatt acctttatat cgtcgcgcga atgttgagga
     4081 aacgtgcccg tacaaagaaa tgttttgccg cttaattttt cagaggctaa tgttgtttcc
     4141 gttttataaa tattaacgcc taaagttgaa agcgtttcga tggtgtttat attttctgca
     4201 tcgccaaaaa attgaaataa ggattgtgct acttttggcc ctatgtcttc caattccgta
     4261 aattgctctt gtgtccacgt ttgaaaatca ctgagttttt ctattttttt tgccaatagt
     4321 tttgctgtgg tgcttccaat gtgccgaata cctaagccaa ctattaatct gtgtaattca
     4381 ttatgtttgg aagcttcaat gccatctttt aaattctgaa tggacttttc tttccagcct
     4441 tctagctgtc ttactttatc atagtcgata gtgtagattg atgtgatgtt ttgtataata
     4501 ttatttgcta cgaatgtgcg tacattttct tctccaaatc caaaaatatc catcgcttgt
     4561 ttggatacaa aatgtataag atgttcttct aattgtgctg gacaattcgt atttatacaa
     4621 cgccaaacac tttcttccag tggttttacc aatgcatgct ggcaggaagg acatgtagta
     4681 ggaaattgaa tttttatttc tgcgccattt cgtttctcag gcacgctgcc tacaatatat
     4741 ggaattacat cgccggcacg ttctacaatt accgtgtcat tgagcattat atttttgtct
     4801 aaaataaaat cttcattgtg caaagaaata gatgatattt cgacacccat taattgaaca
     4861 ggttcgattt tagcaacagg cgtaatggca cctgttctgc caatttggaa ttccacattt
     4921 aatagttttg aaaatgcttg tttcgcttta aatttatagg caacagccca ttttggatgg
     4981 tgtgaagttt ttccgatgag ttgttgttgg tgtatgctgt ttacttttat caccatgcca
     5041 tcgatatcga aatggtaagt gtctctttta ttttcccaat atttacaaaa tgtttcaaca
     5101 tcaagaatag aagtaaatat ttttttttca tggaaaggcg tcttaaagcc taatttttct
     5161 aagatttcta agttcttatt gtgtgtttga aaaatatctg ttatatcaat gccagctgca
     5221 ttttcggcat atccaatttg ataaattatc gcgtctaact tgcgcgctgc tacttcacta
     5281 ctgtctttta aacgcaggct gcctgctgct gtgttgcgtg catttttata caagtccaat
     5341 tcttttttgt tttcagcacg taattgttcg ttttgttttt tacgatcttc attgagcttg
     5401 ttcaacatgg caagttccaa taatacttca cctctgacct cgattttagc aatgccatat
     5461 tcagaaaaat ttgcttttaa tggtattgtt ttaatggctt ttgcgttcaa cgtgatttca
     5521 tcacctgcca cgccattgcc acgtgttgca gcacgcacaa gcctgtcatc ttgatagatt
     5581 aaagcaatac tgctaccgtc aaattttggt tctgcaatat attcgactgc tgcttgctga
     5641 gttaatgctt tttttacttg tgtgtcaaag tcttctaagt ctgtgatgtt gtacgaatta
     5701 tttaatgaca tcatcggtac taaatgttgt acagtcggga aatcattgtt caatccgtgt
     5761 gcgatacgct gtgttggcga gtctggtgtc actaaattcg gaaaattttc ttctagttct
     5821 ttcagctttt taaaaagcct atcgtaatca taatcagaaa ttaaagaatt tgattttaca
     5881 tagtattgat tgtcgtgaaa atttatcact gttttaatgt catcgcaaaa ctgctccgat
     5941 tcttgttctg ttacgaaatc atctatcttg tttaataaag atgctgttaa tgatatacgc
     6001 gaatcaatgc tattaacatc agaaaataaa tcgttgctca tacaagtgaa tctatagttt
     6061 ttgctaaggt aaaatctttt tctgtaaccg tgtttcctgc atcgtgtgtt tgcagcgata
     6121 tttttacttt gttgtaaaca ttgtatattt ctggatgatg attttgcttt tctgctacga
     6181 tagcaatctt agttaaaaat gcaaaagctg caacaaaatc tttaaaaata aattcttttt
     6241 cgagtttatt atcctttgta ttccaagtcg tcataaaatt attttatctt gtataaagtt
     6301 aaatgataaa tatgaattgg aaactgcgtt ttttgttatg gtttgtaaat aatgtacaac
     6361 ctttacgcga ggtagaaaat aaagatattg ccaaagagag aagaaatacg ggacttgcat
     6421 caaatttagg caagttgtta ttcgacaaaa aaataaaagt gtatagcgtt gaagatgccg
     6481 tttttaaagg tgttcctgtt cgtatataca gaaattcaaa acaagcaaat caaaaagtga
     6541 tattgtattt tcatggtggt ggctttgttt tttataacat agaatcacat gattatgtaa
     6601 cgcgtcgttt atgtgcgatg aacgactgca tcgtaatttc tgttgattat agattggcgc
     6661 cagagtattc atttcctgct gcacatgaag atgcgtacaa agtcatttcg tatgtgcatg
     6721 aacatgcaga aaatttattg attgataaca ataaaattat tgttgccggc gatagtgctg
     6781 gtggcaattt ggcagcatgt gcagcccatc atttcaagaa aaataaagaa ataaatattg
     6841 cagcacaagt gttgatatat ccgtggattg atggcaaaat tgcatctgat tcaatcgaaa
     6901 aatatcaaga aggttatctg ctaaccaaga aagcaatgta ttggtttcaa caacaatata
     6961 cgcccaaatt ggaagatcgt ttaaatcctg aggtatcgcc aatttatcat aaagacttta
     7021 agcaattgcc accagctttt gtgcttactg ctgagtatga tcctttaaaa gatgaaggga
     7081 aattttatgc agatacattg aaagaaaatg gaaatgttac aatctataaa gactataaac
     7141 atttagtaca tggctttttt aatttgccta aattgtctga agaaagcatg caagcatatt
     7201 acgatattca agcattttta agaaaagtag tctaactatt ttttgtcagt actcaatacc
     7261 attttcaatt ttagcttggc accaatcgac ataatatcga ctaaatcttg tatgctcaaa
     7321 ttttctgcga cacgcaacac tactgtagga tcaggattat tttctaatgc tgcagaaagt
     7381 gcattttcta tgccttctgc aggaatttct gttgtattaa tgaaataatg tttgtctgct
     7441 gtaacagaaa gtgtgacagg ttgttttggt aatttctcag tggtattggc ttttggcaac
     7501 atcaatttga tgacattagg atttgccatc gtagaaataa ttaggaaaaa caacatcaaa
     7561 aagaacatga tatcattcat ggacgccatt gcatcttcat cgtgaaacgg tcttcttctt
     7621 tttatattca tgttacttta ttcattgcaa acaatcgcat tatgattgct tgttgttaaa
     7681 aaattatttt acaggttttt gtatgcctct tacaaattca aaaatttgct tttgcaattt
     7741 catagaataa ttatcaatct tggttaataa gaaatgataa cccataaaag caataacacc
     7801 aacaactaaa cctgcggccg aagtaatcat tttttcatat aaaccagcag agattgtacc
     7861 aatagaaatg tccgctgatt gtgagatatt ataaaagatt ttgattacac ctaaaattgt
     7921 accaataaaa cctaaacgtg gcgctacacc tgatatcata cttatccagc tcatgcgttt
     7981 ttccatttca ttaagttcaa tattcgattg aatatctaat gctttctcaa tatgatctat
     8041 cggtttgcct aattgcgaaa tcccagcttg taacacacta cctaaggcag atttgtcagc
     8101 agtacataaa gcatcagcac cttgcatatt gccagcatat aatttgtcta atacttcgcg
     8161 aactaagttg tcgttgattt ttgaacgctg actaaaaaat agatatcgtt caatgataaa
     8221 aaataaagcg atagccaaaa atgccactaa aggtatcatg ataacaccac ctttcgtaag
     8281 tagtcctaac acagataact cttgtactgg tggagccgct gccggcataa tagcatttgc
     8341 tgtatctaca acggtgcttg ctacttgtgc agcttgcaat aaaattccca tatacttcta
     8401 atattttaac aaaaataaga ttttactatg aacgttcaaa tacaatatac tattgtgttg
     8461 tggataactg aaaatttatt ttcttttact atatttattt ctaaatatta atgtatgttt
     8521 tccaggctac aatatgtgat attgattatt tgtgtttctt tttctttcgc tgaagctcaa
     8581 acaaattcta tgcctgaata tgatttaaat aaaaattatc caggctcaca atataaattt
     8641 tccttcgttc agctgtcaga catacatatt ggtgaaggtt ttggcgatta tggtacaagc
     8701 ggttatttta atgataccat tcctgttgtc gatacaagcg accctgcaaa tgacttgcgc
     8761 gctgcaattc gctggattaa tgaaaataaa atgaataaga acttgaaatt tgtcgtcatt
     8821 tcaggagatt taactggaag cgccgagaaa tcagaatttc aaatgtgtaa aaatttatta
     8881 aatacactcg aaattccata cgtgcctatt attggcaatc atgatatttg gccttatgtt
     8941 cgctatcaag acgaagcgcc ttatgcttgt ggcgattcgg taatgaatga aatatttgat
     9001 gatgtttaca cacaaaatgc gagttttttt tctaattgga atgatggctc tcgccttcag
     9061 agaacttaca atccagaaac tcagagagaa cattatctac aaaatttttc ttttgaatac
     9121 gataatttca ttttctatgg gttggatttt aatccacgtt atcatgtcaa taaagcagag
     9181 cctggcattg gccctgaagc acaactaatg gattggaatg gtggtacttt tcagtggctt
     9241 aaaaatgaac tgacaaataa tccaagaaaa ggcaataaga acatttgttt tatctcgcac
     9301 catccagcaa ctgacaatat cttgtttata ctttcaggtt ttgtttttga tgctaatgaa
     9361 tatataaaaa tgatggacat gcttacgcct tttaaacaac atttaggctt gtggatggct
     9421 ggtcatattc atattgatta taattatcct ttggtcaata atattatgcc tgttattgga
     9481 attgctgcca ataaagattt tgattcggca cattttagaa ttgtgaatgt atatgaaatt
     9541 ccgactgcaa cagctattcg tacgaatgta aattcctcca aattacaagt gtttcccaat
     9601 ccaagtaatg gacttttcca tgttagtggt attgcattat ctaatacagc tactattcaa
     9661 atatttgatg tattaggtca gtcagtgtat tctaaaaata ggaatgaaat gaatatgcaa
     9721 gaaaatgagt attcaattga tatttcgaag tttccaaaag gcgtgtattt gttgcatgta
     9781 aatgatggcg ctagcgaata tttccaacaa ttgatattgc attaatcatt ttgccatcca
     9841 aacttccatg tcttcttggt cgatttctgg caagatacat ttttttacta cgatactatt
     9901 ttctaaatac attacgatag gcaaacttac acctgctact tttatccaat tttctggccc
     9961 taaaaataaa ttgtgtggaa ttgccttgga atgtgtttcg tcaaaaaagg taggcaactt
    10021 ttttttattg ccattaagtg caaaatagat tggcaatgca ggatttcttt tgtgaataat
    10081 cgctaatttt tgtgctgcaa ctctacaatg cgagcaggtt aaacttaaaa atgcaacaat
    10141 atgttttccc tgtcttagtt caatgtttgg gcgttctttc tgtgtagcac tatacattaa
    10201 atctaaaggc actttataat ttacgcgtgc tgtgtcaata gatgaactgt attgtatatt
    10261 aataggataa agaaaaaatc cgatacagag tgatatcagt agtaacaagg gtacaacaaa
    10321 cttcaggtgc tgatttttcc atatcgaaaa atcagataat agatagcata atacacatga
    10381 aatgattaaa acgatatttt ttattatacc ttcaagaggc gacatcttaa aatattcacc
    10441 gaagcaacca caattgcccg tgttgccaac actgataatt ttatacagta aatagaaact
    10501 aaaaacactc agtaatgcaa tggcaaattt tattgtaaat tttctaattg caatatttaa
    10561 tacgagtagt atgcctaaaa agaactcaag gctgataaaa acacgtgcta gatatggcgc
    10621 tgttttccaa tttgcgatgc cgatatctac gaactgaaat tcgaacggct ccatcggata
    10681 tattttcgta atcgacgaaa taataaataa aaatccaact gatgcactta ataaaagtac
    10741 gattgctttt ttcatatagc aaagctacaa aaactgtgtt tctgtgttat aaaaatttat
    10801 gaaatagaag tatcacttaa ttttacaggt gacactcgaa ttgattttct ttatatcact
    10861 ttctctttgg tcacatactt tttttgcatc tagcattttt gtacttggat atgtttcgac
    10921 aacttgtgtc gtagtgccat cggtacactc acatttccag tcttttacac atgaagtagc
    10981 aaaaatgcaa atgagtgcta ctgcaattag aattttattt ttcatagtgt gcatttttat
    11041 taatattact aaaaaattac taaacaagca atattctgat gtataataat gtaatttttg
    11101 gaaacctaat ttttttgata catttgcata attaaatttt ttaaaatgcc agtagtacaa
    11161 ttcaaccaag tatcagaaga agaaaaagaa cttttatata atgcaacggc attagtaact
    11221 tatctgattg gtggtgctga taataatttt gatgaaaaag aaagagcgca gtcagcacat
    11281 ttggtaaaca tcagaacgga acaaggcgac ccaatgttat ttgattacta tcttgatttg
    11341 caagttgatt ttgctgaaag agtagcaaaa gtagaggcaa aatatggcgt tgattcggta
    11401 aatgatagaa cggctctttt agttgctgaa ttagaaaaat taaacgatat cttaccgaaa
    11461 ttagatgata tctatgctag agcttattta aaagcattgc gcactttagc aaaaggcgtt
    11521 gctgaggcat ctggaggctt gttaggtttc ttagaaattt cttacgaaga agagcactta
    11581 atgggcttaa ccatgattac attcgaatag aaatgcctat cgatcatcaa ataaatggca
    11641 aaccaattcc tgaatctttg caaaagatag caaagattac aaagttaatg gatagtcaat
    11701 ttcgaattcc aggaacaaaa tttacttttg gtatagatcc aatcatcggc ttgattcctg
    11761 gtcttggcga tttaattgat tatgtaattt ctgcttattt attaattgcc atggttcgta
    11821 atggtgctag cggtcgtgcc gtagcaaaaa tgattattaa tatcaccatc gatggtattg
    11881 ttggacttat tccattcttt ggacatatat ttgatgtatt ctataaagcc aatcgtagaa
    11941 atttggtatt agcagtagag cattttgaag aaggaaaaca tcaaggaagt gcatggccaa
    12001 ttgtgttgcc tattctgtct gttttattgc ttgtttttat attgtttgcc gtcttgactt
    12061 tttatgttgt aaaatatatg ctcatatttc tatttccaac aggcggaata taaaaaaaag
    12121 gttcagcaaa gctgaacctt tttttatctt actcatcatc gtcatcgtcg tcgtcagata
    12181 catcatcatc atcatcatcg tcgtcatcgt cgtcgtcatc gtcgtcgtcg tcatcatcgt
    12241 catcggagaa tccatattcc aaatcatctg cacttttaac tttgccttta gcttttcttt
    12301 ttggagaatc atcatcatcg cttttgtcaa aatcgtcaaa gtcgccacca gcaccaacag
    12361 aaatttcttt ttcactagaa gaagctacat tgtcgaataa ttcatcttca tcgtaatcat
    12421 ctaccatttt ttgtaattgt gtacttactt tcaccaaata ggaatattct cctaattcaa
    12481 taggcacagc agatacaagt tcttttttag cattagtgaa tctgataatt cctttgttgt
    12541 aaccatgtgg atacttttca tacaacgctt ttaacacttc gtcaggaaca tttttgtagt
    12601 caacaatcac ctttttaaat ccaccaggag gtggcgtagg tgctattttt acagcaggtg
    12661 ctttttcttc taatttactt tttttcggtt cttctttttt agtgcttact tgctcaacag
    12721 tctttgtagc aactgctact tttttaggtg cttctactgg agtcgttact ttttttacaa
    12781 gctttgtctc ctcatttttt ttaatgggtt ctgctttctt agttgttgtt tttgcagata
    12841 cagtttgttt ggtcacagcg ttggagggtt ttgtgttttt aacaggtgat ttagatttta
    12901 cagcaggtgc aggtgcaact tttttcggtg ccacctttgg tgctgctttt ttaacattag
    12961 attttttttc ctctactttt ttcggtgttg ccttttttgc aggagctaca ggagttactt
    13021 ttttagcaac aggtgtaggc gctgcttttt tggcagttgg cttcgcacta attttagcct
    13081 tagaaacact tttagtctct ttcgaagcaa gcgtcttctt agcaacaggc tttgctgcca
    13141 ctgacgtttt ctttggttct gcttttttag tattttttcc cactgacagt aagttgttta
    13201 tgagttttaa aatcattggc taaatgtaaa atgaatttga ccaattatgc aaattatttt
    13261 gtaaaaaaaa taaaaaatat ttttaaacta tcgtttctgt ttaaggtcta atagaaaaag
    13321 agaccatgaa aaagctacta attttaattg taataagcct tgtgatgtta cattcacaag
    13381 ctcgaccaaa agtgtatgtg aaggtgaata aaaatgaaca ccgttacgta aaagtttgcc
    13441 cttcagctac aaatgttgta gtggtaaagc cagcatgtcc aagtcctaag catatttgga
    13501 ttgatggcga ttggatttgg aatcctcaaa tcaatcagta tgtatatgta caaggcaaat
    13561 ggataattcc aatttctaca aaatcaattt ggattgctgg ccattggaaa aatagcagat
    13621 atggctggca ttgggtgcca ggttattgga aataatacac tatcttttca atataattct
    13681 tatgcttttt agtaattaca gcacttaatg ctaagttcaa gtgatgaatg cattacttta
    13741 cttagctaat tcataacatt cctttaatct atctcttctt acttttgggt tgagcaataa
    13801 atttgttaac tcaaaataat aatcatgata gaaacagata tttgtataat aggcgctggt
    13861 cctgttggtt tatttaccgt atttgaagct ggtttattaa agttaagatg tcatttggta
    13921 gattatttgc cacaacctgg tggtcagctt tctgaaattt atcctaaaaa acctatctac
    13981 gatattccag gatttccaac tgttgatgca caagagttag tagataatct tttagaacaa
    14041 ggaaagccat tcaatcctac tttcacttta ggtgagcgca ttgaaacaat aaataaatta
    14101 gaagatggtt cattcgaatt gtttacacac ctcgatacta aaatacatgc aaaagttata
    14161 tgtattgcag gcggcttagg cgtttttgaa ccacgtaaac cagctatcga aaatttaact
    14221 gcttacgaaa aaaatggtgt cgaatacatg gtgttagatc ctgagaaatt tagaaataag
    14281 aaaattgtaa ttgctggcgg tggtgatagc gctttagact ggacattatt cttagcagat
    14341 gttgctgcgg aattgacaat ggtacaccgt ggcgaaacat ttagaggcgc acctgacagc
    14401 gtggataaag tcaagcaatt agctgcagaa ggtaaaatta atcttgcact cagctcaaat
    14461 attacacatt tacacggaga tggtaaatta gaaaaagtta ccattacaca caacgatact
    14521 tcaactaaag aagtagaggc tgataattat cttgcattat ttggcttaag tccaaaatta
    14581 gggccaattg ctaattgggg cttaaatatc gaaaagaatg caatcaatgt agatacgcgc
    14641 gattattcta ccaatataaa aggaatttat gctattggcg atatcaataa ctatgaaggt
    14701 aaattaaaac tgatactttg tggtttccac gaagctgcta tcatgtgtca tagcgctgca
    14761 gagcttattt caggaaaaaa acctacattg aaatatacga cagttaatgg tgtaaattcg
    14821 ttttaataat tattcaattt taatatgcat attgtttgct tgtagcaaac gattaagtta
    14881 tggatgcagt aacaattcat attcaacaag ctgatttttc atttcaagct ttagaaattc
    14941 cattaggcgt agatttgtca ttgatggaga ttctaaaagg agaggagcat ccaatcgaag
    15001 ctatatgtgg cggtatggcg atttgtggca cgtgcagagt ggaagtatta aacaaagatg
    15061 aaattatgga aagcggccca aatgatgacg aattgtgtat gctcgaaacg cttccatgtt
    15121 acacaggcaa ttgcagactg tcttgccaga taaaaatttc agaaaaaata gacggtttaa
    15181 aaattagaat tcctgtagaa aatagctaat ggatacctaa tttccgattt cttacaaaca
    15241 ttctaactgt ttttggttac tttattgttt gtaaatttta gttgttaaca ataatgcaat
    15301 tctatttaat ttctattgta gatatttgct atagttttgc gctcgaaaaa atagagcctg
    15361 cagcaaaaat tgacattatg aaatatgcat gtaatataat tctacttatc atattaagta
    15421 gtttaagttt aaacgttctt gcacaacatc aaaaagatac tacaaaactg attgataaac
    15481 tcaaagatat tccacaagta gtagtaagaa aatcaaataa ggaatttaca attaaaggta
    15541 atattaccga caagctgacc aaagaacctt taattggcgt aaatattttt gtagagacag
    15601 ataaagtgcg tggtaccgtt accgattttg atggtaatta tgaactaaaa gccaacgaag
    15661 gtgatgttat tgtatttacc tatatgggct tgcgcgatga aagatataca gttactcagt
    15721 cacaaacata caatttgata atggaagaag aaggtaatgt gatggacgaa gtggttattt
    15781 ccgtaggtcg aaaaaaagaa aaagctttag acgcacctgc atctatcgtt tctgttgatg
    15841 caacagcaat aaaaaataaa gccggtcttg gtattactga tcatatcaaa aattcagctg
    15901 gtgtgcaaat tactagcaca ggtattcaag gtggacagcc ttcagttcgt ggttttgcag
    15961 gttatttcaa tagcgatgta atgtcattaa cagatacaag agtggcaaaa ctacctaata
    16021 caggtttgaa tatgtatcag atgatgacca ctactgatgc tgatatcgaa cgtatagaag
    16081 tgttaaaagg tccagccgca gctttatatg gtccaaacgc caatcaaggt gtgatacata
    16141 ttatcaccaa atcgccaata aaataccaag aaacaaaatt ttggacatct atcggcgctc
    16201 gtgttaaaat caaagataca ttgcaagata tcaatcgtga aaatccaaga tttgattacg
    16261 ataaaattgc aaaacgtatg attttctctt taggatttat ggttagtgat actattcatt
    16321 ctaaaaatcc aaatgtaaaa atgggaatga aagtttccag tcaattcttt accggccacg
    16381 actggcgtta tagtgatgta ggcgatcctg cttacgtaat tagatttaaa gcgggacgag
    16441 atactattta taatgaacgt ccagatgaaa cgacagatcc aagtggaaaa ggtgtggaag
    16501 tagctaacga acgcgatgag aaaattttga atggtcaagt agatgcacga tatgatatac
    16561 aaatcaaaga agattttaat attgtgttag ctggtggcgt caatgtcagt aatggcatta
    16621 ttccgagtcc agtcggtgct atgcaaaata aaaattgggc ttactcttgg gcacaagtac
    16681 gcttcaactg gaaaaattta tttgtgcaag cttacatgaa tggcaataat tcgcataaaa
    16741 catatttcgt tccagttggt ggtagatata ttgacaaatc acacatgtat tctgtacagg
    16801 ctcaatattc tcatgatgta tttaaaaaac gtatcaactt aatttatggc ttcgatgcat
    16861 tttttacacg accaaatact ggaggtactt tgaatggaaa atatgaagac aaagatgcta
    16921 ttgacgaata tggtatttat atgcaaggcg attatagatt acatcctcgt tttaatatca
    16981 ttgttgctac tcgtttagat tataatacag ccgtaaaaaa agtattattt tcaccaaaag
    17041 tggcaatagt ttataaacca ggaactggac ataatttgcg ttttacattt aatagagcat
    17101 ttaaaaatcc agcatcagca gcgttttatg tcgatgcaaa acaaggcgaa attccaggtg
    17161 gtattcaagt tcgaaatgtg ggcacgccac gttccggatt taattatagc ttcaatgcta
    17221 atccaaattt tgatggtgca atgttgccac agtttttatc gccatttggc acatcaggag
    17281 aatttcatga cgtaggcgat aaatcgttta atacgacagg ttgggacggc attttatctg
    17341 ctattcgtgg tcagtttggc ggtcaggctg gcatcgatga taattcacca atattgccta
    17401 ttatcaattt agtagttaat tctattattc ctcaaactat tccaggtgat attaatcatg
    17461 tggtaaaaaa tctgaatacc gcaaaacaag tttttgaatc taataatgat agatggaaaa
    17521 aaacagaaga cattaaacct ttaaaaccta ccactaccta tacgtacgaa ttaggctaca
    17581 aaggtgtaat tggtaaaatg tttggtatgc aaatcgatgt atatagaaca gattataaag
    17641 actacttagc accagtaact ttgcttacac cagcagttat gttcgacgat gaacagttaa
    17701 tggattattt aggacctcga atattgcaaa attataacaa cttaaatcca gtcattcaat
    17761 tagccatgaa tgcattctta gatcaaagca aaaaattcaa cggcaataat aacggtacag
    17821 caagtgatga gtttgtaagc ttaattcgta gagccgtgca aaacctacca attggtgtga
    17881 ttacacctaa agaagcggaa ggaccaaata tgctattagt aacacgaaat atcggcgacg
    17941 ttgtcgtata tggtgtagat tttaactcga cattatattt gccaaaaggc atttcaattt
    18001 tagcaaatta ctctttcgtt gataaagatt ctgtaaaggt ggaaagtgct cctttaggtt
    18061 atgtagcgct taattcacca aaacatcgcg tgaatgctgg tgtgaattat aatgcagaaa
    18121 aaataggttt caatatcggt ttgcgtttta aatggagcgc aggtttccct gtattatctg
    18181 gtaatttcat cggccgagtg aagccaacac atgatatgga tttagatatt tcgtacacac
    18241 caaaattcat gaaagatcat tttaatatga ctgtgtctat atcaaaccta tacaatagaa
    18301 aacaacaata ttttataggt gcgccagtaa tgggattaat gggcgtatgt aagttgacat
    18361 acacacttta atttttttat ttttatttaa aagcattaat tttgcaaatc atttgaagca
    18421 aattcaagat tattcaaaga ttttattatg caaattttta aacaattttc tttcgattcg
    18481 gcacatttct tacctaatgt gccagtaggc cataaatgca aagaaattca tggccacact
    18541 tataggctta cagtatatat cgaaggtgaa ttggacaatg aattaggttg ggtaattgat
    18601 ttcgctgatt taaaacacgt tatagagcca gttataaata gcatagacca caaactatta
    18661 aatgaaattc ctggcctaga taatccgaca tgtgagcgaa ttgctatttg gatttggaac
    18721 caaataaaac cgaatgttcc gctactttcc aaaattgaat tgtatgaaac actgagctca
    18781 ggcgttgtat atgaaggcaa ataaaatagg catgctccac caaaaa
//
LOCUS       DAOQQV010000044        18608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_911_length_18608_cov_8.172910, whole genome shotgun sequence.
ACCESSION   DAOQQV010000044 DAOQQV010000000
VERSION     DAOQQV010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18608
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_18608_cov_8.172910"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PLL59_06200"
     CDS             complement(<1..1047)
                     /locus_tag="PLL59_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HMY23433.1"
                     /translation="MHDIKLDKSILKRNFNKNSLALKFTTKIVAIFLFFFTKNEVFSQ
                     LSLESIFLQQEYKLQPSPTIQFLHQQSNYVKVLNEKNETLLEFYNKEYKKINDWKISK
                     TSTSSAIRDVSFSNTDKYILARTDCAPLFRHSLACRYFISDSSGMLQPINSNKVLFPS
                     FSPDDKFIAYISNNDIFYKNIDNSRETQITSDGEWNKIINGKGDWVNEEEFALSKAYE
                     WNPKSNQIAYIKFDESEVKTFSIPEYYDMQYPNYFNYKYPKVGEKNAKLSVHIYQLKN
                     KKTKSVSIPYAYEYIPRIYWNKSGTELIMLLMNRNQDTMRLVAYNTSSKMARLLYLET
                     DTAYVNIPIALTFLN"
     gene            1042..1716
                     /locus_tag="PLL59_06205"
     CDS             1042..1716
                     /locus_tag="PLL59_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23434.1"
                     /translation="MQKFYLLLYVAFFMSGSHLFAQEEIYDSPIRIMDTLERNPDSKK
                     GKEKPDKIDVADDAKTALEKKKEMLNRLRIGGNFGLQFGSYTYINLSPTLGFMAVKKR
                     LEVGAGPIFIYQRFRYTNFTSYNFFVYGADVYSHGFLYKGLYLEARYDAVNKPSYYNL
                     DKRLWVHHLLLGAGYAVEVGKVGVFNISLMYNVLDNQESIYQGTFSDKFPLILNMGFG
                     FGVGGN"
     gene            complement(1771..2094)
                     /locus_tag="PLL59_06210"
     CDS             complement(1771..2094)
                     /locus_tag="PLL59_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23435.1"
                     /translation="MKTKNVITAIIINALGISIAFAGGTMPHAKNRQIHQQERIYNGV
                     KSGELTKKEAIKLEIQQNNIQQAKQAAKADGIITAQEKAKIEYKQDKASNNIYEQKHD
                     TQNRP"
     gene            complement(2175..2846)
                     /locus_tag="PLL59_06215"
     CDS             complement(2175..2846)
                     /locus_tag="PLL59_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23436.1"
                     /translation="MKKIPFPFQLLQISCISFLVLTAIAMFFFPGGTILDHNTKGYSF
                     FNNFFSELGRWRTHTGETKWISFIGFELALVIHSIAMFVFNIYFLKSTDSIKLNKSLY
                     YIALISGSIFPFLLTGIAFTPCDLVLPQHMDFVYAAFGTLIPLSFSYTFLVRQHHILP
                     NKYGNVLMIIVVSIALYLLLMIFGPSPKTVPYVQQTAQKIIVYAMIFSLLYLTRGCKK
                     YLSVA"
     gene            complement(2949..4916)
                     /gene="gyrB"
                     /locus_tag="PLL59_06220"
     CDS             complement(2949..4916)
                     /gene="gyrB"
                     /locus_tag="PLL59_06220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HMY23437.1"
                     /translation="MSEEVLEEVKKNAQYGAENITVLEGLEAVRKRPAMYIGDINVRG
                     LHHLVYEVVDNSIDEALAGHCKNIFVTIHEDNSISVLDDGRGIPVDMHPKEGRSALEV
                     VLTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSTLLRATVYRDGKEYQQEFSIGIPK
                     YPVKEIGASDKRGTKIHFKPDDSIFTVTEYNYDTLAARMRELAYLNRGISITISDERN
                     KDENGNIFSENFYSEGGLREFVTFLDKTRQPLIPEVIYMEGAKDNVVVEVAFQYNDSY
                     NENIHSYVNNINTIEGGTHVVGFRRALTRVFKSWATKNDLFKNVKTEISGEDFREGIT
                     CLVSVKVPEPLFEGQTKTKLGNSEVDAIVASVVGEHLEYFLEEHPKEAKIIVNKVILA
                     ATARMAAKKARELVQRKNVLGGSGLPGKLSDCSDKDPGACELFLVEGDSAGGTAKQGR
                     NRAFQAILPLRGKILNVEKAMEHKIYENEEIKNIFTALGVTKGIEGDEKALNIVKLRY
                     HKIVIMCDADVDGSHITTLILTFFFRHMKELIENGYIYIASPPLYLVKKGNKQQYAWN
                     DEQRKRFIKELAGEGREDSVHVQRYKGLGEMNAEQLWETTLNPETRTLKQVTIESAAE
                     ADNIFSMLMGDEVPPRREFIETHAKYAKIDV"
     gene            complement(5205..5723)
                     /locus_tag="PLL59_06225"
     CDS             complement(5205..5723)
                     /locus_tag="PLL59_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMY23438.1"
                     /translation="MKLAFLYTFILLYFTSCLNNSSKLHVLMEDANGLEEGSKVSCKG
                     VNVGTIDVIKIHNNNVVATIVLNDDFKATKGTTAQVSLDNIFGKRSLILLPGASTSFL
                     ANNDTIIAQSGKNISIVDNLLEKGNVDSLLHNINLDKFHLDSLKNKVDSLDIDGLLKK
                     AEKILDFNKLLN"
     gene            complement(5734..7044)
                     /gene="murA"
                     /locus_tag="PLL59_06230"
     CDS             complement(5734..7044)
                     /gene="murA"
                     /locus_tag="PLL59_06230"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HMY23439.1"
                     /translation="MSADAFEVYGGKPLNGTIIPQGAKNEALQILSAVLLSEEDITIS
                     NIPNILDVNVLIELLSEMGVSVNKINDDTYTFNAKAVDPDILLTENFKRRAGHLRGSV
                     MILGPLLARFKKAYLPTPGGDKIGRRRLDTHFLGFVELGAKYHYDKDNSFYSLSTEKL
                     IGKYILLEEPSVTGTANIVMAAVLAEGTTTIFNAACEPYLQQLCGMLNRMGAKIKGIG
                     SNLLIIEGVQKLGGTTHRLLPDMIEIGSFIGLAAMTKSTITIKDARVDMLGIIPTYFQ
                     KLGIHLEIKSDDIIVHAQEKYEIQTFIDGSILTISDGPWPLFTPDLLSIILVTATQAD
                     GSVLIHQKMFESRLFFVDKLIDMGAKIILCDPHRATVIGINREYNLRGINMSSPDIRA
                     GVSLLIAALSAEGKSIISNIHQIDRGYQYIDTRLQALGADIKRL"
     gene            complement(7050..7742)
                     /locus_tag="PLL59_06235"
     CDS             complement(7050..7742)
                     /locus_tag="PLL59_06235"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HMY23440.1"
                     /translation="MNYNTSRNDIEMREYGRHVQKMVDYAVSLQDRKERQAVAENIIQ
                     LMSILNPQLRNTADYKHKLWDHIFIISDFKLDVDSPYPIPTAATARIKPSFLPYPQKR
                     IKLKHYGKNVEALVKKAIDMEDEDKKEIFTEIIGNFMKLAYKNWSNEEVSNDLIKEDM
                     KTLSGGELIISEDMNIESMTRNQQKQRSFSKNNNKNRNNNNRNNNNRNNNNRNNNNRN
                     NFQNNNNRNRYK"
     gene            complement(7939..9018)
                     /gene="lpxK"
                     /locus_tag="PLL59_06240"
     CDS             complement(7939..9018)
                     /gene="lpxK"
                     /locus_tag="PLL59_06240"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HMY23441.1"
                     /translation="MRIITKILLFPFAVIYGAIIWLRNWLYKSNFIGSTDFEVPVISV
                     GNLSVGGTGKTPFVALLIEILYGKYSAAVLSRGYKRSTKGYLEVNINHSAKDVGDEPL
                     MLKLKYPHVQVSVGEQRVFAIPHLLASHHDTQVIVLDDAFQHQSVRPDINILLTTYEK
                     PFYDDAILPIGTLRECSSGKQRANIIVVTKCPADLSITQQNLIRKKINIHQKQKLFFS
                     TIEYGTAYNILQPNEKYNVAENKSHLLITGIANASHLRQYLEEKKVDVVHFEFSDHHY
                     FEMHELEKIKQNYPTLCQWLTTEKDVVRLALHKEWFLQNEITLFCMPIRAKIIGENAA
                     DFQKDIIGFLDYFYAQENISAVLND"
     gene            9033..10127
                     /locus_tag="PLL59_06245"
     CDS             9033..10127
                     /locus_tag="PLL59_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMY23442.1"
                     /translation="MKIAEIIQTIEDFAPLSYQESYDNAGLLIGDKMSTCTGVLICID
                     VLEAVLDEAIRKKCNLIVAHHPIVFSGLKKINGNNYIERIVLKAIKNDIAIYACHTNI
                     DNVQNGVNAIIADRLGLINRRVLLPSKNVLKKLYTYVPEVNKAMLMDQLFAAGAGNIA
                     NYSECSFSVAGVGTFKGNAQSNPVLGKKNVRSVEAEHKIEVIFPKHLESKIIKTLLHY
                     HPYEEVAYEVISLDNHYQQIGSGMIAELKTAMPTKTFLAMLKQEMKAKCIRHTSLVKK
                     KIKRVALCGGAGSFLLPNAIAQEADIFISGDFKYHQFFDADNQIIIADIGHFESEQFT
                     VQLFNKIISEKFPTFAVRISTINTNPINYS"
     gene            10127..10897
                     /locus_tag="PLL59_06250"
     CDS             10127..10897
                     /locus_tag="PLL59_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HMY23443.1"
                     /translation="MARKKELTTVADKLEQLEELQAIHSKIDAIHILKGELPIEVADL
                     EDEIEGTNKRIAKVQGEINEAKEEIEHRKTLTKEAQSLITKYDKQLHNVKNNREFDAL
                     NKEIELQKLEIQLAEKKMKDANFTIENHQKQLDEIQKRLDSKEKDLELKKKELQNIIK
                     DTEKEEQELNVKAKEVEVDVEEKFLLAFNRIRKTYKNGLAVVKVSRGACGGCFGYIPP
                     QTQSEIRTKKTIMTCEHCGRIIAGVDDKDVYISEEEMA"
     gene            complement(10984..12681)
                     /gene="gldG"
                     /locus_tag="PLL59_06255"
     CDS             complement(10984..12681)
                     /gene="gldG"
                     /locus_tag="PLL59_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HMY23444.1"
                     /translation="MKRHYFIQSIKALSISIFGILCINFISSFFYHRFDLTAEKRYTL
                     TTSSKKLLNGLDGKIDIEVYLTGKDLPAGIKILQNETRELLQEFRNNSKGLVAFHFFD
                     YNSIKDIKKREEFQMELVKKGLRPTNLEVKSQDGFTEKLVFPGAIIKNNGREIPIQLL
                     ENQFSVGAQGSLNNSIGFLEYKLANGIQKITKEHPVKVAFLQGHGELGIEPLQEFLKA
                     LSEQNFILDKLELDKDKLLNNNIDILIVAKPQKAMSEYEKFLIDQYIMNGGKTLWLLD
                     NIISDLDSFKLAPSIVATPRELNLDDMMFRYGVRIEHNLVLDLYCNQIPIIENIGGNP
                     QPKLFPWVFYPIAVTKNNHPIVKNLDPVALKFVSSLDTLNNPDVRKTVLLSTSTYART
                     QPTPFQLYLEGAKQKPNPYLFNQKDIPMAVLLEGNFSSFYKNQFTTDLQKLLATQQIQ
                     FQAKSGLNKMIVISDGDIASNDLEANGVPLALGYDKYSRKLYANKDFLVNCVEYLVDE
                     NNLIDARNREVKMRLLDKAKVLENKILWQIITFGLPLTLILIFGIGYHIYRQRKYAK"
     gene            complement(12716..13444)
                     /gene="gldF"
                     /locus_tag="PLL59_06260"
     CDS             complement(12716..13444)
                     /gene="gldF"
                     /locus_tag="PLL59_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HMY23445.1"
                     /translation="MTAIIKKEINLFFSSLIAYISMAVFFIALALNLFIFQGNILDSN
                     YATLDTFFTLAPWILLFLIPAITMRSFADEKQSGTIELIATKPITDFQIVVGKFIAAL
                     CLWTFTFLPTLIYFICVKTLSLTEAPIDSGATYGSYIGLYFLGAVFVAIGIFSSAITN
                     NQIVAFLIGVFLCYVMYDVFFRISSLPIFQGQLDYWIQSIGLNAHYDSISRGVVDTRD
                     IVYFLSVISLFLIGTKTALESRKW"
     gene            complement(13473..14390)
                     /gene="gldA"
                     /locus_tag="PLL59_06265"
     CDS             complement(13473..14390)
                     /gene="gldA"
                     /locus_tag="PLL59_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HMY23446.1"
                     /translation="MSISVQNLSKWYGQQKAVDSVSFELKKGEILGFLGPNGAGKSTT
                     MKMLTCYIPPSDGTAKVAGIDIIENPLLVKEKIGYLPENNPLYYDMYVKEFLEFALHI
                     NRPKQSSNRQQRIKETIDIVGLGIEQHKKIGQLSKGYKQRVGLAQALIHDPEILILDE
                     PTTGLDPNQLADIRSLIKTLGREKTILFSTHIMQEVQAVCDSVVIINKGKLVANDKTD
                     NLQKLWSSDVVVEVEFEQAITIEQLQQINGVQLVNKLSDTNFVLHGKDSTQLRKDIFT
                     IAVNTNNPLLTLKQESKSLEDIFQQLTHP"
     gene            14487..15026
                     /locus_tag="PLL59_06270"
     CDS             14487..15026
                     /locus_tag="PLL59_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23447.1"
                     /translation="MKSFSTYKYLIILLFIAISTACSVGNKVIPNPGGISGVDTSNTT
                     LNAFQIIVTGDTSFIMSITSIDSLNGVYDDSLIVAGLELSGPTLAGGVGFKTFSSSTG
                     DYLTEETPPGINTAQFGILKTIRGVRKIFFMNHGRISITEHNLADKTVKGSFDVNNEF
                     NLTGDIYLRCRGNFYIKYQ"
     gene            complement(15034..16218)
                     /locus_tag="PLL59_06275"
     CDS             complement(15034..16218)
                     /locus_tag="PLL59_06275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMY23448.1"
                     /translation="MKKILVLTPTLPYPPVSGGVIKSNKVIQFLLEHYSVSVACFLKN
                     EDPLYLQEFKVIFPLQNFIAEELNVTRNAINLIKSNILGIPLNLYRNKSKTLKEKIKA
                     IIHQHDVVFCDHYVMFQYIPEDYKGKIILHEHNCEYLIWKRYAAIEQNIAKKLALLNQ
                     AYRIKRYEQEICAKAHVVLAAPNDQEELVKIGADANKFMETYHLGDDSLLQAANIEFS
                     KTKKSLLYIGTLSWEANIDGLLWFYDKVWPILKKQDSELTLSIIGKHADARLQEMAAK
                     DDDIILTGFVEDVEPYFQQHRVFITPLRFGSGIKVKVVNALYRGIPCVTTSIGAEGLK
                     VKDAEHLFIKDTPEAYAAAIQKLLTDEECWNAIRNNAREIANQYYTWHAVLATIKDAV
                     EK"
     gene            complement(16356..18230)
                     /locus_tag="PLL59_06280"
     CDS             complement(16356..18230)
                     /locus_tag="PLL59_06280"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23449.1"
                     /translation="MKQVFITYIITHFVFNVFGQIQITKPNPKLYFDAKYVTLENSTP
                     QKPDSLIDNFHRYNTTEKQFAPYNNLGNYGTAYYSLMFNHNNFIGFKHGFNSFDLYFI
                     QPEKIKYFNTKTPYTQLDFVFGGKEEIVGGAEFAINIRPNVNLGFSFHRNSFKGKSQH
                     QLSIHNLFSIQNTYQSKNKRYDLKIAFIYNGIKNEENGGWSQTDVFTNPFYKKNKSFV
                     SVHLDDASNRYNERNVNLHQQINLGKKVATQINDSTTRKIVKQAKYAIVHDFELSHWK
                     FIYKDFEKDSSFYSSLIFDEDSTHDATKTWKLSNAIYFKNNQQDSHPKKFLFAIGAQY
                     DFIKYQQRYTNEFTHDIQLKAKIYNKTDLAFFGYTVTAAIDLAPKYIGDFEFNFLGKL
                     NFKKTISVALAGNVSLASPTRKQDSYLGNHYAYHTDFKKTFQVLAQAEFAWKKQLLFA
                     TIQNYFIQNYIYNDTASLPAQYNKPLNALVVKFRKDFNTKHIYSGTELYAQWISNRDI
                     VRLPVFAMKQTLYYKGGWISGKLNAQLGFDIMYNTNFKGNAYNPALAEFYLQNKETLK
                     FYPIMDLFFRLQVKFTNIFFRVEHVNQGMFKQKGIFTAPNYGYLDRTFRVGVLWQFYD
                     "
     gene            complement(18335..>18608)
                     /locus_tag="PLL59_06285"
     CDS             complement(18335..>18608)
                     /locus_tag="PLL59_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HMY23450.1"
                     /translation="QKQEAATPKPTYEAPKHLTKINSAAKSTATITSDLKALQAGMQV
                     LHEKFNAGKVISVEGSGENRIATIFFDGVGNKKIMLKFAKLQIIES"
BASE COUNT         5759 a         3097 c         3250 g         6502 t
ORIGIN      
        1 gtttaaaaat gtgagcgcaa taggaatatt tacatacgca gtgtccgttt caaggtatag
       61 cagtcttgcc atttttgagc ttgtgttata ggctactaag cgcattgtat cttggtttct
      121 attcattaag agcattatta attcagtgcc acttttattc caataaatgc gtggaatata
      181 ttcgtatgca tagggaatcg aaaccgactt tgttttcttg ttttttaatt gataaatatg
      241 cacactcagc ttggcatttt tctcgccaac ttttggatat ttgtagttaa aataatttgg
      301 atattgcata tcgtaatatt ctggaatgct aaaggttttc acttcgcttt catcaaattt
      361 tatataagca atctgattgc tttttggatt ccattcgtat gcttttgata aagcaaattc
      421 ctcttcattt acccaatcac ctttgccgtt aataatctta ttccattcgc catctgatgt
      481 gatttgtgtt tccctactat tgtctatatt cttataaaaa atgtcgttgt tactgatgta
      541 agcaatgaat ttgtcatctg gagagaagga aggaaaaagt actttgttgc tatttattgg
      601 ctgaagcata ccactgctat cgctaataaa ataacgacaa gcaagtgaat gtctaaataa
      661 aggtgcacag tctgttcttg caagtatata cttatcagtg tttgaaaatg aaacatctcg
      721 aatagcactt gaggtacttg ttttgcttat tttccagtca tttatttttt tatattcctt
      781 gttgtaaaat tcaagtaaag tttcattttt ttcattaagt actttaacat aattggattg
      841 ttgatgtaga aattgtattg taggtgaggg ctgaagttta tattcttgtt gtaaaaaaat
      901 agattctaag ctcaattggc taaatacttc attcttagta aagaaaaata aaaatattgc
      961 aactatttta gtggtgaatt tcaatgctaa actattttta ttaaagttac gttttagtat
     1021 agatttatct aattttatat catgcaaaaa ttctatcttt tactatacgt tgcttttttt
     1081 atgagtggca gccatttatt tgcacaggaa gaaatatatg acagtccgat acgcattatg
     1141 gacactttag agcgtaatcc agatagtaag aaaggcaaag aaaaaccgga taaaattgat
     1201 gtagcagatg atgcaaaaac ggcattggag aagaaaaaag aaatgctgaa cagattgaga
     1261 ataggtggaa acttcggctt gcagtttgga agttatacct acatcaattt gtcgccaacc
     1321 ctaggtttta tggcagttaa gaagcgttta gaggtaggtg caggtccgat atttatatat
     1381 caacgattta gatataccaa ttttacttct tataatttct ttgtgtatgg tgctgatgta
     1441 tattcacacg gttttttgta taaaggccta tatttagaag cacgttatga tgcagtaaat
     1501 aagccttcct attataattt agataagaga ctttgggtgc atcatctctt actaggcgca
     1561 ggctatgctg ttgaggttgg caaagtcgga gtttttaata tttctttgat gtataatgtt
     1621 ctagataatc aagaatctat ttatcaaggc acgttcagcg ataaatttcc tttaatactt
     1681 aatatgggct ttggtttcgg tgtaggtgga aattaaaatg agcttatttc ataagaaata
     1741 agctcaccaa aattacacta ctataaatat ttacggacga ttttgtgtat cgtgtttttg
     1801 ctcgtaaata ttattcgatg ccttatcttg tttgtattca atttttgctt tctcttgtgc
     1861 agtaataatt ccatcagctt ttgctgcttg cttagcttgc tgtatgttgt tttgttgtat
     1921 ttctaattta atagcctctt tttttgtgag ttcgccactt tttacgccat tgtagattct
     1981 ttcttgttga tgaatttgtc tattcttcgc atgtggcatt gttcctcctg caaatgcgat
     2041 tgatatgcca agtgcattaa taataattgc tgtgattaca ttctttgttt tcatgatgtt
     2101 cattttaaat gattaattaa ttgtttgcta ctttgactgc aacaattgtc aatggtttaa
     2161 aatgaatata gaaattaagc tacgcttaaa tattttttgc atcctcgtgt taaatataac
     2221 aaggaaaaaa tcatggcata tacaataatt ttttgagctg tttgttgaac gtagggtact
     2281 gttttcggac ttggaccaaa tatcatcaat aataaataga gtgcaattga aaccacaatt
     2341 atcattaata cattgccata tttattaggc aaaatatgat gttgcctaac aagaaatgtg
     2401 tatgaaaagc tcaatggaat caaggtgcca aaagcagcat atacaaaatc catgtgttgt
     2461 ggcaacacta aatcgcaagg agtgaaagca atgcctgtta ataaaaatgg gaaaatgctt
     2521 ccactgatta atgcgatata atacagtgat ttatttagtt tgatgctgtc tgtacttttt
     2581 agaaaataaa tattgaaaac aaacatcgcg atggaatgta ttaccaatgc taattcaaaa
     2641 cctataaaag atatccattt cgtttcgcca gtatgtgtgc gccatctgcc taattctgaa
     2701 aaaaaattat tgaagaaact atatcctttt gtattatggt cgagtatagt gccaccaggg
     2761 aaaaagaaca tcgcaatggc tgtcagtact aaaaatgaaa tacagctaat ttgtaatagt
     2821 tgaaagggga aaggtatttt cttcatcaat taaaaataag aaaataaacg cattgtcgtc
     2881 aaatattaga taaagaaaaa atccgttcgc atagaacgga ttttattatt tcaaaagtgt
     2941 attaaatctt acacgtcaat ttttgcatat tttgcatgtg tttcgataaa ttctctacgc
     3001 ggtggcacct catcgcccat caacatagag aaaatattgt cggcttctgc tgcgctttct
     3061 attgttactt gtttcagcgt tcttgtttca ggatttaatg tcgtttccca aagctgttca
     3121 gcattcatct cacctaaacc tttgtaacgt tgtacgtgta cgctgtcttc tcttccttct
     3181 ccagccaatt cttttataaa gcgcttacgt tgctcatcgt tccaagcata ttgttgcttg
     3241 ttgccttttt ttactaaata caaaggcggc gaagcgatgt agatatatcc attttcgata
     3301 agctctttca tgtgtctgaa gaagaacgta agaattaaag tggtgatgtg agagccatca
     3361 acgtcggcat cgcacatgat gactatttta tgataacgta gttttacgat attaagtgct
     3421 ttttcgtcac cttcgatgcc tttggtaaca cctaatgctg taaagatatt ttttatctct
     3481 tcattttcat aaattttgtg ttccattgct ttttctacat tcaaaatttt accacgcaat
     3541 ggtaaaatag cttgaaatgc acgatttctg ccttgttttg cagtgccacc agcagagtca
     3601 ccttcgacta agaacaactc acatgctcca ggatctttgt cggaacagtc tgatagtttg
     3661 cccggtaatc ctgagccacc taatacattc ttgcgttgta ctaactcgcg tgcttttttt
     3721 gctgccatac gagcagtagc ggcgagtata actttattta caattatctt agcttctttt
     3781 ggatgctctt ctaaaaaata ttctaagtgt tcacccacaa cactagctac gatggcgtca
     3841 acttctgagt tgcctaattt cgtttttgtt tgtccttcaa ataatggctc aggtactttt
     3901 acagatacta agcaagtaat accttctcta aagtcttcac cacttatttc tgtttttaca
     3961 tttttaaaca aatcattttt tgttgcccaa gatttgaata ctcttgttaa tgctcttcgg
     4021 aaaccaacca catgtgtgcc accttcaata gtgttaatgt tattgacata agaatgaatg
     4081 ttttcgttat aagagtcatt atattgaaat gcaacttcaa ctactacatt atcttttgca
     4141 ccttccatgt aaattacttc tggaattagt ggttggcgtg ttttgtctaa gaaagtgaca
     4201 aactctcgca agccaccttc agagtagaag ttttcagaga aaatatttcc attttcatct
     4261 ttatttcttt catcggagat agtgatgctg atgcctctat ttaaataggc taattcacgc
     4321 atacgtgctg ctagtgtgtc gtagttatat tctgtaacgg tgaatatgct gtcatctggt
     4381 ttgaagtgta tttttgtacc gcgtttgtct gaagcgccaa tttcttttac aggatatttt
     4441 ggaataccta tgctaaattc ttgttgatat tctttgccat cacgatagac tgttgcacgt
     4501 aaaagtgtgg ataaagcatt aacacaagat acaccaacgc cgtgcaaacc accagatact
     4561 ttataagaat ctttatcgaa tttaccacca gcatgcaata ctgttagtac aacttctaat
     4621 gcagaacgtc cttcttttgg atgcatgtcc acaggaatac cacgaccatc gtctaaaaca
     4681 gagatggaat tatcttcgtg aattgtaaca aaaatatttt tacaatgacc agctaatgct
     4741 tcatcaatgg agttatccac tacttcatat actaaatgat gcaagccacg tacgttgatg
     4801 tcgccaatat acatggcagg tcgtttacga acggcttcta aaccttctaa aacagtgatg
     4861 ttttccgcgc catattgcgc atttttttta acttcttcta gtacttcttc gctcattttt
     4921 ttaaggtatt gatggttgag tttgtagtgt atgtatttta tatgttttat atagaaaaaa
     4981 taatcacatc aaacgtctga acatagacag acaaagatac aaaatgatag tctaaatcag
     5041 gcaaaatagc agctatatta tccacatttg ctaagttaaa aaaatgtaat tttgaacgta
     5101 aagaagtatt gaattaccgg ctattagtgt aatgctgtta catttctatt ttttcaataa
     5161 aattagaaga aagaaaacga tgttgagttg aaaaattgga ttttctaatt caataatttg
     5221 ttgaaatcta ggattttttc tgcttttttt aataaaccat ctatgtcaag tgaatctacc
     5281 ttgtttttca atgaatccaa gtgaaattta tccaaattga tgttgtgtaa aagcgaatca
     5341 acatttcctt tttcaagcaa attatctacg atgctaatat tcttgccgct ttgtgcaatg
     5401 atagtgtcat tgttagctaa aaatgaggtg ctcgcaccag gcaaaagtat caaacttctt
     5461 ttaccaaaaa tattgtctaa tgatacttgc gcagtcgtgc cttttgttgc tttaaaatca
     5521 tcatttaata ctatggtggc gactacattg ttgttatgta tttttatgac atcgatagtg
     5581 ccaacattta cacctttaca gctgacctta ctgccttctt ctaagccatt tgcatcttcc
     5641 atcaatacat gcagtttcga gctattattt aaacagcttg taaagtacaa tagtataaaa
     5701 gtataaagga aagcaagctt catgattttc attttatagt cgtttgatgt ctgcacctaa
     5761 ggcttgtaag cgtgtgtcaa tatattgata acctctatct atttgatgaa tattggagat
     5821 gatgcttttt ccttcggcag acaaagctgc aatcaacaat gaaacaccag cacgaatatc
     5881 tggtgaactc atattgatgc ctcgcaaatt atattctcga tttatgccga ttactgttgc
     5941 acgatgtggg tcgcacaaga tgatttttgc gcccatatca atgagtttat cgacaaaaaa
     6001 caatctggat tcgaacattt tttgatgtat taaaacgcta ccatcagctt gcgtagctgt
     6061 gaccaaaata atactcaaca aatcgggtgt aaatagcggc catggtccat ctgaaatggt
     6121 taagatagaa ccatcaataa acgtttgtat ttcatatttt tcttgtgcgt gcacgatgat
     6181 gtcatcgctt tttatttcta agtgaatgcc taatttttgg aagtaagttg gaatgatgcc
     6241 gagcatgtca acacgagcat ctttaattgt aatagttgac ttcgtcattg cggctaagcc
     6301 aataaaactg cctatttcta tcatgtcagg caataagcga tgtgtagtgc cgcctagttt
     6361 ttgaacgcct tcgataatta atagattcga gcctattcct tttatttttg cacccattct
     6421 attgagcatt ccacatagct gttgtagata tggctcacaa gcggcattaa atattgtcgt
     6481 tgtgccttcc gctaaaacgg ctgccatgac tatatttgct gtgcctgtaa cagaaggttc
     6541 ttcaagtaaa atatatttac cgattagttt ttcagtgctt aacgaataga aagaattgtc
     6601 tttgtcgtaa tggtattttg cgcctagctc aacaaagcct aaaaagtgag tatctaatct
     6661 gcgacgacca attttatcgc cgccaggcgt aggcaaatat gcttttttaa agcgcgctaa
     6721 aagtggtcct aatatcatta cggagccacg taagtgacct gcgcgtcttt tgaaattttc
     6781 tgttagtaaa atatcaggat ctaccgcttt agcattgaaa gtataagtgt catcattgat
     6841 tttattgaca gaaacgccca tttctgaaag caattcaatt aagacattta catctaaaat
     6901 attgggaata ttagaaatgg taatatcttc ttcagacaat aagacagcag ataatatctg
     6961 caaagcctcg ttttttgcac cttgaggaat gattgttcca tttaatggct ttcctccata
     7021 aacttcaaaa gcatcagcac tcataattat tatttataac gatttcggtt gttgttattt
     7081 tggaaattat ttcgattatt attatttcga ttattgttat ttcgattgtt attatttcga
     7141 ttattgttat ttctgttttt attattgttt tttgagaagc tgcgttgttt ttgttggttg
     7201 cgtgtcatgc tttcgatatt catatcttca ctgataatca attcgccgcc agacaatgtt
     7261 ttcatgtctt ctttaattaa atcattgctc acttcttcat tgctccagtt tttgtaagcc
     7321 agcttcatga agttgccaat gatttcagta aatatttctt ttttatcctc gtcttccatg
     7381 tcgatagctt ttttcaccaa ggcttctaca tttttaccat agtgttttaa cttaatgcgt
     7441 ttctgtggat aaggcaaaaa ggaaggcttt attcgagccg ttgcagccgt tggaattgga
     7501 tatggagaat cgacatctaa tttaaagtca gagatgataa atatatgatc ccaaagcttg
     7561 tgtttatagt cagcagtatt gcgtaattgc ggatttaaaa tgctcattaa ttgtataatg
     7621 ttttctgcta cggcttgacg ctcttttctg tcttgcaaac tcacggcata gtccaccatt
     7681 ttttgtacgt gacgaccgta ctcgcgcatt tctatatcat ttctactggt attgtaattc
     7741 attgttattg ttgattatta ttattgttga ttattaatat tattatttaa aaagcattgt
     7801 ttattagaga tgaaataaac cgaaagatgt tgatgaaaag taaaacttac tatgcgctag
     7861 aatggcctga ataagcttac gaaggcaaaa caaaaataat aaaatatttc ggtattattt
     7921 catgtgtcga ttaaaaattt aatcatttaa cactgctgaa atgttttcct gtgcatagaa
     7981 ataatctaaa aatccaatga tatctttttg aaaatcagca gcattttcgc ctattatttt
     8041 cgctcttata ggcatgcaaa ataatgtaat ttcgttttgt aaaaaccatt ctttatgcaa
     8101 tgccaatcgc acgacatctt tttcggtagt caaccattgg cataaagtcg gataattttg
     8161 tttgattttc tctaattcat gcatctcaaa ataatgatga tcactaaact caaaatgcac
     8221 gacatctact tttttttctt ctagatattg cctcaagtgc gaagcattag caattcctgt
     8281 gatgagcaga tgacttttat tttctgctac attatatttt tcattgggtt gcaatatatt
     8341 ataagcggtg ccatactcga tggtgctaaa aaataatttt tgcttttggt ggatatttat
     8401 ttttttacga atcaaatttt gttgagtaat agataaatca gcagggcact tcgtcacaac
     8461 tataatattt gcacgctgct tgcctgaact acattcgcgt aatgttccaa ttggtaaaat
     8521 agcgtcgtca taaaatggct tttcatacgt agtcaacaga atattaatat caggccgtac
     8581 actttgatgt tggaatgcat cgtctaacac gatgacttgt gtatcatgat gtgatgcgag
     8641 taaatgcgga atggcgaaaa cacgctgctc accgacactc acctgcacat gtggatattt
     8701 cagtttgagc atcaaaggtt cgtcgccaac atctttagcg ctatgattaa tatttacttc
     8761 aaggtaacct tttgtcgagc gtttgtaacc acgactcagc acagcagcgg aatattttcc
     8821 ataaagtatt tcaattaata atgcaacaaa tggcgttttt ccggtaccgc caacacttaa
     8881 attgcctact gaaattacag gaacttcaaa gtctgtagaa ccaataaaat ttgatttgta
     8941 cagccaattg cgaagccata ttattgcgcc atagattaca gcaaacggaa ataatagtat
     9001 ttttgtaatt attctcaaaa taaattaagc ctatgaaaat agcagaaata atccaaacta
     9061 tagaagattt tgcaccatta tcgtatcaag aaagctatga taatgctggc ttattaattg
     9121 gagataaaat gagcacttgc acaggtgtgt tgatttgtat cgatgtgtta gaagcagtgt
     9181 tagatgaagc catacgcaag aaatgtaatt taatagtggc acatcatcct attgtatttt
     9241 ctggtctaaa aaaaataaat ggcaacaatt atatcgaacg tatcgtattg aaagccataa
     9301 aaaatgacat tgccatttat gcttgtcata ccaatattga caatgtacaa aatggtgtca
     9361 atgcaattat cgcagatcga ttaggcttga taaatagacg tgttttgctg ccaagtaaaa
     9421 atgtactcaa aaaattatac acgtatgttc ctgaagtgaa taaagctatg cttatggatc
     9481 agctttttgc tgcaggtgct ggaaatattg ccaattacag tgaatgtagt ttttctgttg
     9541 ctggtgttgg cacattcaaa ggcaatgcac aatccaatcc agtactgggt aaaaaaaatg
     9601 tgcgtagtgt agaagcggaa cataaaattg aagtcatttt tcctaaacat ttggagtcga
     9661 aaattatcaa aacactacta cactatcatc cgtatgaaga agtcgcatat gaagtaattt
     9721 cattagacaa tcactaccag caaattggct ccggcatgat tgcggaactg aaaactgcaa
     9781 tgccaacaaa aacattttta gcaatgctta aacaagaaat gaaggcaaaa tgtattagac
     9841 acactagctt ggtgaagaaa aaaataaaac gtgtagcttt gtgtggaggc gcaggaagtt
     9901 ttttgttgcc caatgcaatt gcacaagaag cagatatctt tatctccgga gattttaaat
     9961 accatcaatt ctttgatgct gataatcaaa taattatagc agatattggt cattttgaaa
    10021 gtgagcagtt tacagtacaa ttatttaaca aaataatctc ggaaaaattt cctacttttg
    10081 cggttcgaat ttcgacaatt aacactaatc caataaatta cagctaatgg cacgtaagaa
    10141 agaattgacc actgttgcag acaaattaga gcaattagaa gagttacaag caatacactc
    10201 aaaaattgat gctatccata ttttaaaagg tgaattacca atcgaggtgg ctgacttgga
    10261 agatgagatt gaaggcacaa acaagcgtat tgctaaagtg caaggagaaa tcaatgaggc
    10321 aaaagaagaa attgagcaca gaaaaacatt gacaaaagag gcgcaaagct tgattacaaa
    10381 gtatgacaaa caattgcaca atgtaaaaaa taatcgtgag tttgatgctt taaataaaga
    10441 gattgaattg caaaaattag aaattcaatt agcagagaaa aaaatgaaag atgctaactt
    10501 tacgattgaa aatcatcaaa agcaattaga tgaaattcaa aaacgattag atagcaaaga
    10561 gaaagattta gagctaaaga aaaaagaatt acaaaacatc atcaaagata cagagaaaga
    10621 agaacaagag ctaaatgtga aagctaaaga agtagaagtt gatgtagagg agaaattttt
    10681 attagcattt aacagaatca gaaagacata caaaaatgga ttagcggtag taaaagtaag
    10741 ccgtggcgca tgtggtggct gttttggtta tattccacct caaacacaat cagaaatccg
    10801 cacaaagaaa acaatcatga catgcgaaca ttgcggtaga attattgcag gtgttgatga
    10861 taaagatgtt tatatttctg aagaagaaat ggcttagtcg tatagccttt tatacaaata
    10921 tacataaaaa tccatctcaa atgagatgga ttttttttat gtttttttca gaaggaaata
    10981 atattattta gcatattttc gttggcgata gatatggtat cctatgccga aaataagtat
    11041 aagtgtgagt ggcaaaccga aggtaattat ctgccaaagt attttatttt ccaatacttt
    11101 ggctttgtct agcaagcgca tttttacttc tctgttgcgc gcgtctatca aattattttc
    11161 gtccaccaaa tactcgacac aattgactaa aaaatctttg tttgcataca gttttctgct
    11221 gtatttgtca taacctaatg ccaagggcac accatttgct tccaagtcat tggaggcgat
    11281 atcgccatcg ctaatgacta tcattttatt taagccactt tttgcttgaa attggatttg
    11341 ctgtgtggct aataattttt gcaagtctgt tgtaaactga tttttataaa atgagctgaa
    11401 attgccttct aacaatacag ccattggaat atctttttga ttgaataaat agggatttgg
    11461 tttttgcttc gcaccttcta agtataattg aaatggcgtt ggctgtgtgc gtgcatacgt
    11521 agaagtagaa agtaacaccg ttttacgaac gtcaggattg ttgagcgtat ctaaggagct
    11581 aacaaatttc agcgctacag gatctaaatt tttgacaatt ggatgattgt ttttggtgac
    11641 cgcaatcgga taaaaaaccc atggaaataa tttaggttga ggattgcctc caatgttttc
    11701 aataatagga atttgattgc agtataaatc caatactaaa ttgtgctcta tacgcacacc
    11761 atatcgaaac atcatatcat cgagattcaa ttcgcgtggt gtggctacga tgcttggtgc
    11821 taatttgaaa ctatctaaat cacttataat attatcaagc agccacaagg ttttgccgcc
    11881 attcataata tattggtcta tcagaaattt ttcatattct gacattgctt tttgtggctt
    11941 ggcaacaatt aaaatatcta tattattgtt tagcaatttg tctttatcta actctaattt
    12001 gtcgagtata aaattttgtt cagataatgc ttttaaaaat tcttgtaagg gttcgatgcc
    12061 taattctccg tggccttgca gaaaagctac ttttacagga tgttcctttg tgattttttg
    12121 aatgccattg gcgagtttat attccaaaaa tccgatagaa ttatttaaag aaccttgcgc
    12181 acctacagag aattgatttt ccaataattg aattgggatt tctctgccat tgttttttat
    12241 tatggcgcct ggaaatacta atttctctgt aaaaccatct tgcgatttca cttctaaatt
    12301 ggtaggtcgg agtccctttt ttaccaattc catttgaaat tcttcgcgtt tttttatgtc
    12361 tttgatgctg ttgtaatcaa agaaatgaaa agcgaccaat cctttagaat tgtttctgaa
    12421 ttcttgcaat aattcgcgtg tttcattttg caatattttg atgccagcag gtaagtcttt
    12481 gcctgtcaga tatacttcaa tgtctatctt tccatctaaa ccgtttagta gctttttact
    12541 tgatgtggtg agcgtgtatc ttttctctgc cgttaaatcg aaacgatgat aaaaaaatga
    12601 agaaataaaa ttaatgcata gtatgccaaa aatactgatg cttaatgctt taatagattg
    12661 tataaaataa tgtctcttca ttttatttgt tcatcaaaaa aaaatcaaat attctttacc
    12721 attttctgct ttctaaggct gtttttgttc caattagaaa taaactaatc acacttagga
    12781 aataaacaat atcacgtgta tccaccacac cacgactaat ggaatcgtaa tgtgcattta
    12841 aaccaatcga ttgaatccag tagtctaatt gcccttgaaa aataggcaaa gagctgattc
    12901 taaaaaaaac gtcatacatg acgtaacaca gaaatacgcc aattaagaat gcaacaattt
    12961 gattgttggt aatggcagag gagaagatgc ctattgctac aaatacagcg cctaaaaaat
    13021 agagtccgat atagctgcca tacgttgcgc cgctgtctat tggtgcttct gtaaggctta
    13081 atgtttttac gcaaataaaa taaataagcg ttggtaaaaa tgtaaaagtc cacaagcata
    13141 aagcagcaat aaattttcca acaacaattt gaaaatctgt aattggtttt gtggcaatca
    13201 actctatcgt gccactttgt ttttcatcgg caaaagaacg catggtaatt gctggaatca
    13261 gaaacaataa tatccaaggt gctaaggtga aaaatgtatc taaagtagca tagttactat
    13321 ctaaaatatt gccttggaaa ataaatagat ttaaagcaag cgctataaaa aaaacagcca
    13381 tgctgatata ggcaatcagc gaggaaaaaa ataaattgat ttctttttta atgatagctg
    13441 tcatgtgtta ttctttgtga tatgctgcaa aattatggat gtgtcaattg ttggaaaatg
    13501 tcttccaagc ttttgctttc ttgtttaagt gttaataatg gattgttggt attaacggct
    13561 attgtaaaaa tatctttgcg cagttgtgtg ctgtccttcc catgtaagac gaaattcgtg
    13621 tcagatagtt tatttactaa ttgtacgcca tttatttgtt gcagttgttc aatagttata
    13681 gcttgttcaa attcgacttc aacgacaaca tcgctgctcc ataatttttg caagttatct
    13741 gttttgtcat tcgccaccaa tttcccttta ttaataatca caacactatc gcacacggct
    13801 tgtacttctt gcatgatgtg tgttgaaaat aagattgtct tttctcttcc taatgttttt
    13861 atcaagctgc gaatgtctgc caattgatta ggatctaagc ctgttgttgg ctcatctaag
    13921 attaatattt cagggtcgtg aattaaagcc tgtgctaatc caacacgctg tttgtagcct
    13981 ttcgaaagct gacctatttt tttatgttgt tcgatgccca agccaacgat gtctattgtt
    14041 tctttaatac gttgttggcg attgctgctt tgttttggcc gattgatatg caaagcaaat
    14101 tccagaaatt ccttgacata catatcatag tataatggat tattttctgg taagtagcct
    14161 attttttctt ttaccaataa aggattttca ataatatcta ttcctgcaac ttttgccgtg
    14221 ccatcactcg gtggaatata gcatgtcagc attttcatag tcgtcgattt tccggcacca
    14281 ttcggtccta aaaagcctag gatttctcct ttttttaact caaatgaaac gctgtccact
    14341 gccttttgct ggccatacca ttttgataag ttttgaacgg aaatagacat aattaagatg
    14401 caaaaataag gaatttaata gcaatcaaaa tcttaaaatt agcaaataat aaaacgatag
    14461 aaaaaatagc tatatttact gcctacatga agtcgttttc tacatacaaa tatttaatta
    14521 ttctactttt tattgctatt agcacggctt gttctgtcgg caataaagtg atacctaatc
    14581 ctggtggcat tagcggcgtc gatacttcga acaccacatt aaacgccttc cagattatag
    14641 taactggcga cacctcattt atcatgagca ttacatcgat agattcatta aatggagttt
    14701 atgacgacag ccttattgtt gctggtttag aactgtcagg gccaacctta gctggtggtg
    14761 ttggatttaa aactttttct tcatcaacag gagattatct aacggaagaa acaccaccag
    14821 gaattaatac ggcgcaattt ggtatcctta aaacaataag aggtgtgcgc aaaatatttt
    14881 tcatgaatca cggtagaatt tcgattacag aacataatct tgccgataaa acggtaaaag
    14941 gaagttttga tgtcaataat gagtttaatc ttaccggtga tatttacctg cgttgcagag
    15001 gaaattttta tataaaatat caataatcct tgcttatttt tctacggcat cttttattgt
    15061 tgccaaaaca gcatgccaag tataatattg attagcaatt tcgcgtgcat tgtttcgtat
    15121 cgcattccag cattcttcat ctgtcaataa tttttgaatg gcagcagcat aggcttcagg
    15181 tgtgtctttg ataaaaagat gttctgcgtc ctttactttt aaaccttcag caccaattga
    15241 tgtcgttaca caaggtattc cacgatataa agcatttacc acttttactt taatgccgct
    15301 accgaaacgc agcggtgtaa taaatacacg gtgttgctga aagtagggct ctacatcttc
    15361 aacaaatcca gttaaaataa tatcatcatc tttcgccgcc atttcttgta agcgtgcatc
    15421 agcatgtttg ccgatgatgc tcagtgtaag ttcgctgtct tgttttttta gtattggcca
    15481 aactttatcg taaaaccaaa gcaggccatc gatgttggct tcccagctta atgtaccaat
    15541 atataaaagt gattttttgg ttttcgaaaa ttcgatattt gctgcttgca ataaactatc
    15601 atcgcctaga tggtaggttt ccataaattt attagcatct gcgccaattt tgactaattc
    15661 ttcttggtcg tttggtgccg ccaaaacaac atgtgctttc gcacatattt cttgctcgta
    15721 acgtttgatg cgatatgcct gatttaataa ggccaatttt ttagcaatat tttgttctat
    15781 agctgcatat cgcttccaaa tcaaatattc gcaattatgc tcatgcaata ttattttgcc
    15841 tttgtagtct tctggtatgt attgaaacat gacataatga tcgcaaaata ctacatcgtg
    15901 ttggtgtata atggccttta ttttttcttt taatgttttt gatttattgc gatataaatt
    15961 tagtggaatg cctaagatgt ttgattttat taggttgata gcattccgtg ttacatttaa
    16021 ttcttctgca ataaaatttt gcaacggaaa aatgaccttg aattcttgta gatatagtgg
    16081 gtcttcgttt tttagaaaac aagcgacact caccgagtag tgttccaata gaaactgaat
    16141 aactttgtta gatttaatca cgccaccgct tactggcgga tacggtaatg ttggcgtaag
    16201 gacaaggatt tttttcactt attttatttg tatatcaagg aaaaacatta acggcatatg
    16261 gttttacaag atacgaaatt gtatttgttt ttgattttat ttatgcaata atatatgtgt
    16321 agctagacat agatggataa gctatagcaa caatattaat cataaaattg ccataatacg
    16381 ccaactctaa atgttctgtc aaggtagcca taatttggcg ctgtgaagat gcctttttgc
    16441 ttaaacatgc cttgattgac gtgttccact ctgaagaata tattggtgaa ttttacttgc
    16501 aatctaaaaa ataaatccat gataggatag aattttaatg tctctttatt ttgtaaataa
    16561 aactcggcta aagctggatt gtatgcattg cctttgaagt tagtgttata cataatgtca
    16621 aaacctaact gtgcatttaa ttttccagaa atccagccac ctttgtaata tagcgtctgt
    16681 ttcatagcaa acactggcag gcgcacgatg tctctgtttg aaatccattg tgcatataat
    16741 tctgtgcccg aataaatatg tttagtatta aaatctttgc gaaattttac cactaaagca
    16801 tttaacggtt tgttgtattg agctggcaaa gaagcggtgt cgttgtaaat ataattttga
    16861 ataaaataat tttggatagt agcaaataag agctgttttt tccaagcaaa ttcggcttgc
    16921 gccaacacct gaaatgtctt tttaaaatcc gtgtgataag catagtgatt gcctaaatag
    16981 ctatcttgtt tgcgcgttgg cgatgctaat gaaacattgc cagcaagcgc aacagaaatg
    17041 gtttttttga aatttaattt acctaaaaaa ttgaactcaa aatctccaat gtattttggt
    17101 gcaaggtcga tggcagccgt tacagtgtag ccaaaaaatg caaggtctgt tttgttgtat
    17161 atttttgctt tcagttgtat gtcatgcgta aattcattgg tataacgttg ctgatatttg
    17221 atgaaatcat actgagcgcc aatagcaaac aagaattttt ttggatgact atcttgttga
    17281 ttgtttttaa aataaatcgc attgctcaat ttccatgttt ttgtagcgtc atgtgttgag
    17341 tcttcatcaa aaattaaact agaataaaat gaactgtctt tctcaaaatc tttgtatata
    17401 aatttccaat gtgataattc aaaatcatga acaatggcat acttagcctg cttcacaatt
    17461 ttgcgcgttg tgctgtcgtt gatttgagtc gcaacttttt tacctaaatt aatttgctga
    17521 tgtagattaa cattgcgctc attataacga ttgctggcat catctaaatg cacgcttaca
    17581 aaggatttat tttttttata aaatggattt gtaaatacat cagtttgcga ccaaccacca
    17641 ttttcttcat ttttgatacc attgtagata aatgctattt ttaaatcgta tcgcttgttt
    17701 ttactttgat acgtattttg gattgaaaac aaattatgta tcgataattg atgctgactt
    17761 tttcctttaa aactatttct atgaaacgaa aaaccaagat ttacatttgg tctaatattg
    17821 atagcaaatt cagctccacc aacgatttcc tctttgccgc caaatacaaa atccaattgt
    17881 gtgtaaggtg ttttagtatt gaaatatttt attttctcag gttgaatgaa atataagtcg
    17941 aatgaattaa aaccatgttt aaagccaata aaattattgt gattaaacat taacgaataa
    18001 taggcggtgc cataatttcc gagattgtta taaggcgcaa attgtttttc ggtcgtgttg
    18061 tatcgatgga aattgtcgat taaagaatct ggtttttgtg gcgtcgaatt ttctaaagtt
    18121 acatacttag catcaaagta taattttggg ttgggtttgg taatttgtat ctgaccaaaa
    18181 acattgaata cgaagtgcgt gatgatatat gtgatgaata cttgcttcac tattcaaaaa
    18241 tacctttttt tattttatca attattgctt ttatagggaa tttattgctg tttaaactta
    18301 tcaaatccaa ttctcaaaag catattattg tgtattatga ttcgataatt tgcagtttgg
    18361 caaatttgag catgattttt ttgttgccaa caccgtcaaa aaagatagtt gcaattctgt
    18421 tttctccact tccttcaaca ctgatgactt ttcctgcatt aaatttctca tgtaatactt
    18481 gcatgccagc ctgtaaggcc tttaaatctg atgtgattgt tgctgtcgat ttagcagcgc
    18541 tatttatttt agttaaatgt tttggtgctt cgtatgttgg ttttggtgta gcagcttctt
    18601 gcttttgt
//
LOCUS       DAOQQV010000045        18002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_959_length_18002_cov_7.735220, whole genome shotgun sequence.
ACCESSION   DAOQQV010000045 DAOQQV010000000
VERSION     DAOQQV010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18002
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_959_length_18002_cov_7.735220"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLL59_06290"
     CDS             <1..861
                     /locus_tag="PLL59_06290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY23451.1"
                     /translation="ENNYSYEIIAVDDGSKDKSWQVITTHAQKNPAIKGIRFQRNYGK
                     SAALQVGFEAAQGDVVITMDADLQDSPDEIPELYKMISVDAYHLVSGWKKKRYDPLTK
                     TIPTKLFNAATRYVSGIHNLHDFNCGLKAYQNKVVKNIEVYGEMHRYIPVIAKWAGFD
                     KITEKAVQHQERKYGVTKFGIERFVNGLLDLMSITFVGKYGKRPMHLFGTLGVLSFFF
                     GVLILVYLTVLKLIFSSEVSLTNRPSFYLAMLTVIVGTQLFVAGFLGELIARNGIERN
                     TYSIGEKINL"
     gene            complement(869..1987)
                     /locus_tag="PLL59_06295"
     CDS             complement(869..1987)
                     /locus_tag="PLL59_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMY23452.1"
                     /translation="MKYLKWLLSFTSRTVNIVFVVLLFISLSASYFSPEKLWFLAFAG
                     MLLPLLLIINSIFVVVWILRKRHFAIVSILAIAACLPQIFRLFASNIGQPKIENISNQ
                     AIKVLSYNVRDFDLYNWSENEKSKEIIFKTLKEKDADVICFQEFYNDTSKEFNTIKQL
                     HDIGYPYYYFTREVVLRNSDEWGIAIFSKTPIAEHGTIMKQSFKTGYGKKPFKGLYAD
                     ITINNKVIRFINVHLQSIYFGEKEYETIADFKETQNLDEHGAKNIFRKLKRGFERRVH
                     QADELRTFIDNQKMPLVVCGDFNDLPNSYVINTVSKNLKDAFIDYGFGIGHTYNGNIP
                     FLRIDYIFCSQTINVQKFEIINNKISDHYPIYSELSLP"
     gene            complement(2033..2953)
                     /locus_tag="PLL59_06300"
     CDS             complement(2033..2953)
                     /locus_tag="PLL59_06300"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMY23453.1"
                     /translation="MTIWQDIKWQYQYGSDIIKLILINCAVFIIVNLISLPFMLMAKN
                     APIDLVRFFSLFASIKNIAFHPWGIFTYMFVHQSFLHILFNMLGLYWFGQIVQDLVGR
                     AKILPLYIFGGMAGGLLYVVAYNIFPAFNAVLDSTYNFGASAAVLAMVLAAATIAPNY
                     ELGLLFFGPVKIKWIAFVYVLLDLINIQNGNAGGHIAHLGGALFGFLYIKLLQNGTDL
                     GRPFYAVVDFFSNLFKKKNTLKVTYKKEKQYAYTAAENQTKTNNTTQQKDTLSKQERL
                     DAILDKINRSSYDSLSKEEKDFLFKISQED"
     gene            complement(2956..3642)
                     /locus_tag="PLL59_06305"
     CDS             complement(2956..3642)
                     /locus_tag="PLL59_06305"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMY23454.1"
                     /translation="MNNLTPVVKNLILLNILVFVACFLYPDLTDILAMHYPTNPAFKP
                     WQLVTHMFTHGGIFHILFNMYALYSFGAVLESDRVLGSKRFAFLYLFSGLGAIAFYLA
                     INAGMAYYYIGDIFFTNETSLPATLNESAIKSLSQIYYASLVGASGAIYGLLAAFGYL
                     FPNAPLMFMFVPVPIKAKYMIPIVIIVFDIMFAQTGIGNIAHTAHIGGAIFGFLLVVF
                     WNKTNKTTFY"
     gene            complement(3739..5619)
                     /gene="mutL"
                     /locus_tag="PLL59_06310"
     CDS             complement(3739..5619)
                     /gene="mutL"
                     /locus_tag="PLL59_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HMY23455.1"
                     /translation="MSDIIKLLPDNIANQIAAGEVIQRPASVVKELLENSIDAGAKNI
                     QLIIKDAGRALIQVVDDGCGMSETDARMCFERHATSKIKKIDDLFSLYTFGFRGEAMA
                     SIAAVAQVEMKTKLHEQEVGTLLIVEGSKVIQQEPVATNAGTSISVRHLFYNVPARRN
                     FLKSNQIETKHILDEFIHVALANPSVFFSLHHNGSEIYHLKSSNIRQRIVNVFGKNYN
                     EKLVPIEEITDYVSVRGFIGKPELSKKTRGEQFFFVNNRFIKNNFLNHAISKAYDGMI
                     ADKNFPFYCLFIDIEPSAIDINVHPTKQEIKFEDEKAIYLLLNAAAKHGLSQYSVMPT
                     IDFEHEATFNLLVDERVYNEQPFKVVQSKLNSDSNVRGSYHSPFDKDIPLTQQKSVST
                     GTNFDDWKKLYDIDEQTEEKTLTIKSKMDLLALENENDISTNPFKNERFEPIQLHERY
                     ILTQIKSGLILIDQHKAHQRILFEKYLRNFASQPSSTQQTLFPKSLEFNNSDAELLKE
                     MLSDLKLLGFDIEFFGGTTFVVHGMPSDLRDENETHLIEKLLDDYKNGAKTEEFDKRK
                     NVAKSLAYQTAIKSGKSLDKNTMQQLIDELFACEQPMINPVGMATFVQYSFDEIERKF
                     GN"
     gene            5817..8738
                     /locus_tag="PLL59_06315"
     CDS             5817..8738
                     /locus_tag="PLL59_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HMY23456.1"
                     /translation="MKNKTFTIFLLTICCMLFAANTKASAPPYLKDTSAVWVDSILQS
                     MSLDEKIGQLFMVAAYSNKDEASYKTIDKYIKEYKVGGLIFFQGTPQKQAELTNRYQA
                     QSKIPLWIGFDGEWGLGMRLDNTISYPRQLTLGAIDNPDLIYEMGAEIARQMKRIGIH
                     INFAPDIDVNSNPNNPVINDRAFGDNKFGVTRKGLAYMFGLQDNKILASGKHFPGHGD
                     TDVDSHKDLPILKHSLERLESVELYPFKQFIRYGIGSIMVSHLNVLALDAAPNFPATL
                     SPKIIQQKLIDSMKYNGLIFTDAMNMKGVAKFYPSGVAEVMAIMAGNDVMLFPEDVDK
                     AIEGVRCAVLQDSIISEETINRKVRKILAAKYWLGLQTYQPIQLEGIDADINNDAAKY
                     LKFKLVEHAITVVKDSARLIPVVNLQKRIANVSIGSSKPSVFSTTLDKYTAIDHFYLP
                     KETTETTFNDVLSKLKDYHLVIIDIQDMSRFAAKNFGVTDNSRLFLNKLNKQNQTILT
                     VLGSPYSLKYFQDFNTIIVGYNEEEEAESICAQIIFGARAAEGTLPVTVGNFKYGDGE
                     CTQSNMRLKYGIPLEVNMNVQKLQKADAIIYKAIDDKIFPGAQLLVAKDGNVIYEKAY
                     GFHTYDNTIATKITDLFDIASLTKVTATVPMIMKMQEQGKININKKLGDYFHYPDGVN
                     KKDINIKEMLCHQAGLQAWIPFYKNTLDSLGNPDTLWYSKVQDSIFSVKIVDSLFLKK
                     SFQDSVWNMIYHSALSPVKEYKYSDLGYYLLQQIIEKTYKDSLQNIVKRYLFAPLGMN
                     YTQYNPRWNAYDLNKIMPTEIENNFRKSLLLGTVHDQGAALLGGVAGHAGVFSNANDL
                     AKYFQLLLNEGTYGANRYYEAATINKFTTKQYPNNRRAIGFDKPTPKGHDGPTCDAAS
                     PESFGHTGFTGTIAWADPTNNLIYIFLCNRVYPNAAVNNLAKQNIRPKIQQAVYDAIE
                     K"
     gene            8899..9624
                     /locus_tag="PLL59_06320"
     CDS             8899..9624
                     /locus_tag="PLL59_06320"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMY23457.1"
                     /translation="MNNVYNELTELFTKIDKALSIKDIDEIASLDKLKEICDSSNEMS
                     SIFEMDSMSYSMVYSNKAAFEYLGITEDTIQKMGFKYIISIIHPDNISSMYNMIKFYA
                     NKENKNLCYTGVFFIKVKNGWEWTYSCMKPAIYNADGTVKYLLSTGCSIDNLLKSHKN
                     YKNLQNNLSFFEAHAAKYLSLTSREKEVLHLIAEEHTSKEIAGMLHISPYTVDTYRKT
                     LIEKLQVKSSIGLARYGVLFSLK"
     gene            complement(9920..10954)
                     /locus_tag="PLL59_06325"
     CDS             complement(9920..10954)
                     /locus_tag="PLL59_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HMY23458.1"
                     /translation="MNASTQNTINFLSKLTVRRVWNMSLVYLSFYLTKYFKKPIQYGK
                     PFSISFEPTTSCNLRCPECPSGLRAFTRPIGMLVPAFFEKTIDELSNHLLYLTFYFQG
                     EPYLNTKFLDMVRYAKQKNIYTATSTNAHYLTDDVAKRTIESGLDRLIISIDGTTQET
                     YQSYRVGGHLYKVIEGAKNMVKWKKELKAKTPYIIFQFLVVKPNEHQIDDLKKIAKEI
                     GVDEVILKTAQVYDYKNGNPLIPENEQYSRYKKNNDGTYSIKNNLENSCWKLWHSCVI
                     TWDGKVVPCCFDKDAQHQLGNLQTHSFSQIWNNDLYKNFRTQLVKGRKEIDICKNCSE
                     GCEVNFNYEV"
     gene            complement(11361..12014)
                     /locus_tag="PLL59_06330"
     CDS             complement(11361..12014)
                     /locus_tag="PLL59_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HMY23459.1"
                     /translation="MDTTIMQFVSKDALHFFSDNKVLLSLVMGLGLSASCGFRVFVPL
                     LAASIATKMGILHLGEGFLWMGSTAALICFGAATIFEIGGYYIPFIDNILDTIMTPAS
                     VIAGTLLTASAILPDIDPMLKWGLGIIVGGGSAGIIQAGTALTRATSTATTAGVANPL
                     VATIEHILAIVGSIFSIIIPFVIAAIVCIVIVVLLFFMIRYFKKHIPKTGMKTAIQK"
     gene            12213..12587
                     /locus_tag="PLL59_06335"
     CDS             12213..12587
                     /locus_tag="PLL59_06335"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HMY23460.1"
                     /translation="MEISGKVFKVLPVESGEGKNGTWKKQAIVIETAHDRFPKKVAVQ
                     FWGDLVNHENFVEGNDISVEFDVESREYMGKWYTDVKAWRINTKSTTQNNTSSANTQQ
                     AAPAHTSADIPVATGADDDLPF"
     gene            complement(12589..13257)
                     /locus_tag="PLL59_06340"
     CDS             complement(12589..13257)
                     /locus_tag="PLL59_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23461.1"
                     /translation="MRKIFLSLIFILPVLGFSQSTLFGTWNNPSVGTLILQANGNGSL
                     DGGAFTFTHTANKIIAFSDDGTFNYYYKFSNGNLVVSGGIFENPVTFSKAKTNTVNNS
                     SKSGSIDKSIVGTWCWTNASATYTSSSSNTRCIVINSNGTYQYTYEGSISGSGGGYYG
                     GSSSQNADQGSWKLHGNTISIVSQNEGAQTLSFQKKNHPKTGDPMIIIDGDAYVSYYQ
                     KNPW"
     gene            13466..15895
                     /locus_tag="PLL59_06345"
     CDS             13466..15895
                     /locus_tag="PLL59_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMY23462.1"
                     /translation="MTHQYKISGMSCNGCRTKVENTLNSIQGVTAKVTLEPPIATITM
                     EKHIPTIELQEALSAVGKYTIEMMHDQHHDADETESTSCCSTTTHTAHQHEVPDSLKN
                     NAGKYYCPMQCEGDKVYDKAGSCPVCGMDLVQIPSADDNDDSTYNELWKKMKVALLFT
                     IPIFLIAMLEMVPALDFTAIMPQIAWNWLQFLLSLPVVFYACWMFFQRAWTSLKTMNL
                     NMFTLIGIGVGAAFLFSVIALLFPSIFPAQFKGHNGTVYVYFEAVSVILTLVLLGQLL
                     EARAHSQTNGAIKALLKLAPTEATLVENGVDKVIAIDAIKKGDLLRVKPGEKIPVDGI
                     IIDGSSNIDESMLTGEPIPVEKKINDKVSAGTINGTKSFVIKAEKIGAETLLSQIIEM
                     VNAASRSRAPIQKLADKISKYFVPIVVLISIITFIVWAIFSKESPYVFAFVNAIAVLI
                     IACPCALGLATPMSIMVGVGKGAQNGVLIKNAEALENMNKVNVLITDKTGTITEGKPS
                     VENIISFDNADTDKLLRFAASLNQYSEHPLATAVVNFAKSKNITVIPVTNFEAIMGKG
                     VIGTIENIKIVVGNKKLMEDEQVVITDKIASQITQEQIKGKTVSFIAVNGAVQAYITI
                     TDAIKSSSKQAIKDLMDQGVEVIMLTGDNHNTAKAVADTLGLKHFKAECLPQDKLNEI
                     KRLQAEGKIVAMAGDGINDAPALAQADIGIAMGTGTDVAIESSEITLLHGDLLGIVKA
                     KKLSHAVMKNIKENLFFAFFYNALGIPVAAGVLFPFFGLLLSPMIAAAAMSFSSVSVI
                     ANSLRLRTKKI"
     gene            complement(16070..16142)
                     /locus_tag="PLL59_06350"
     tRNA            complement(16070..16142)
                     /locus_tag="PLL59_06350"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16107..16109),aa:Lys,seq:ttt)
     gene            complement(16228..16605)
                     /locus_tag="PLL59_06355"
     CDS             complement(16228..16605)
                     /locus_tag="PLL59_06355"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HMY23463.1"
                     /translation="MKRSLIIVLLLSIFAACETSSKQLGVKFSEKNTITVDDLIARIN
                     TNPVVNDIQISGKIDKSCRSEGCWFTVKDKEGDEILFDVKDKLFKVPTNSPGKSVVIL
                     ADAAQDSTTEQKFTLSVKGIMFK"
     gene            complement(16612..17205)
                     /locus_tag="PLL59_06360"
     CDS             complement(16612..17205)
                     /locus_tag="PLL59_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HMY23464.1"
                     /translation="MLLFFTACSNKQKVEVKNAQGVVVESYFVDKKKPELKIGVYHKF
                     YDDGKMLEESHFTDGKLNGKRTLYHPNGKIMQTENYVDNKYDGAFTIYYEDGSLQQEG
                     TYKDNMMNGVVKNYFKSPKNKVKEEISMKDNHVNGIYKEYYDNGNIYAMGTKKEIMED
                     IDVFDGEVQIFDSLVNNKLIRKLQFENGRQISKEEIK"
     gene            17298..>18002
                     /locus_tag="PLL59_06365"
     CDS             17298..>18002
                     /locus_tag="PLL59_06365"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HMY23465.1"
                     /translation="MHELQQIIENAWSDRSLLQETATLTAINEVITLLDSGKLRVAAP
                     TTEGWKVNDWVKKAVILYFPTQKMETIEVGPFEFHDKIPLKTNYAALGVRVVPHAIAR
                     RGAYLEKGVIMMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEP
                     VQAAPVIIEDDCFIGSRCIVVEGVRVEKEAVLGANVVLTMSTKIIDVSGSEPKEYRGV
                     VPARSVVIPGTIPKEFP"
BASE COUNT         5977 a         3059 c         3190 g         5776 t
ORIGIN      
        1 gaaaataatt attcgtatga aatcattgcc gttgatgatg gcagtaaaga caagtcatgg
       61 caagttatta caacacatgc acaaaaaaat cctgccatca aaggcatacg atttcagcgc
      121 aactatggta agtcagcagc cttgcaagta ggcttcgagg cggcacaagg cgacgtggtc
      181 attacgatgg atgccgattt acaagattca cctgacgaaa ttccggaatt atataagatg
      241 ataagtgtcg atgcttacca tcttgtgtca ggctggaaaa agaaacgata tgatccattg
      301 accaaaacaa taccaacaaa attatttaat gcagctacgc gatatgtttc aggtattcat
      361 aatttacacg attttaattg tggtctaaaa gcctatcaaa ataaagtagt aaaaaacatt
      421 gaagtatatg gcgaaatgca cagatatatt ccagtgattg caaaatgggc tggcttcgat
      481 aaaatcacag aaaaagcagt acagcaccaa gagcggaaat atggcgttac aaaatttgga
      541 atagagcgct ttgtaaatgg cttgctcgat ttgatgagca ttacttttgt cggaaagtat
      601 ggcaagcgtc caatgcattt atttggcaca ttaggcgttt tatctttttt ctttggtgtg
      661 ctaattctcg tctatctaac ggtattaaaa ttaattttca gcagtgaagt ttctctcact
      721 aatcgacctt cgttttattt agcaatgctt actgttattg taggaacgca gttgtttgta
      781 gcaggatttt taggcgaact tattgcacgc aatggcattg agcgcaacac ctattccata
      841 ggcgaaaaaa tcaatttata agcaacgatt agggcaaact caattcacta tatattggat
      901 agtgatcaga aattttatta tttataattt caaacttctg cacattgatt gtttgggaac
      961 agaaaatata atcgatacgc agaaatggaa tattgccatt ataggtatgt ccaataccaa
     1021 atccataatc tataaatgcg tctttcagat ttttagaaac ggtatttatt acataagaat
     1081 tgggcaagtc gttaaaatcg ccacaaacca ctaaaggcat tttctgattg tcgataaatg
     1141 ttcttagttc atcagcttga tgcacgcgac gctcaaatcc tctttttaat tttctgaaaa
     1201 tgttcttagc accgtgttca tctaagtttt gcgtttcttt aaaatcggca atcgtttcat
     1261 attctttctc tccaaaataa atcgattgca aatgcacatt aataaagcgt atcaccttgt
     1321 tgttaatcgt gatgtcagca tataaacctt tgaaaggttt tttgccatag cctgttttaa
     1381 aagattgttt cataatcgtg ccatgctctg caattggcgt ctttgaaaag atggcaattc
     1441 cccattcatc actattgcgc aatacgactt ctcttgtaaa ataatagtat ggataaccta
     1501 tgtcatgcag ctgtttgatg gtgttgaatt ccttactggt gtcattataa aattcttgaa
     1561 agcaaatgac atcagcatct ttttctttca atgttttaaa aataatttct ttcgattttt
     1621 cattttcact ccaattgtat aaatcaaaat cgcgaacatt atatgataat actttaatag
     1681 cttgatttga aatattttct attttcggtt ggccaatatt gctggcaaat agtctaaaaa
     1741 tctgaggcaa acaagctgca atcgccaaaa ttgaaacaat agcgaaatgt ctttttctga
     1801 gtatccatac gacaacaaaa atgctattaa tgattaataa taaaggcaat aacatgccag
     1861 caaaagcgag aaaccataat ttttccggac tgaaataaga tgcggataga ctaataaata
     1921 gtagaacaac aaatacaata tttactgttc tggaagtgaa agacaaaagc cactttaggt
     1981 atttcatttt ccaaattaaa ttaaataatc agtaaaaaca tagcatttaa tattaatctt
     2041 cctgacttat tttaaacaaa aaatctttct cttctttgga taagctatca taactcgagc
     2101 gattgatttt gtccaaaata gcatctaagc gttcttgctt gcttaatgta tctttttgtt
     2161 gtgttgtatt attggttttt gtctgatttt ctgctgccgt gtaggcatat tgtttctctt
     2221 ttttgtacgt tacttttaat gtattttttt tcttaaataa attggaaaaa aaatcgacaa
     2281 cagcataaaa tggcctacct aaatcagtgc cattttgcag taattttata tacaaaaaac
     2341 caaatagcgc accgccaaga tgtgcgatgt gaccacctgc atttccattt tgtatgttaa
     2401 ttaaatcgag tagtacatat acgaatgcaa tccattttat cttcacaggt ccaaaaaaca
     2461 ataaacctaa ttcgtaatta ggtgcaatcg ttgctgcggc aagcaccatc gccaacacag
     2521 cagctgatgc accaaagttg tacgtgctgt ctaggactgc attaaatgct ggaaaaatat
     2581 tataagccac tacataaagc aggccacctg ccatgccacc gaagatatat aatggcaata
     2641 tttttgctct gcctacgagg tcttgcacaa tttgtccaaa ccaatataag cctagcatat
     2701 taaacaaaat atgcaagaaa ctttgatgca caaacatata cgtaaaaatt ccccaaggat
     2761 ggaaggctat atttttaatg gaagcaaata gtgaaaagaa acgcactaaa tcgataggtg
     2821 cgttttttgc cattaacata aatggtaagc taataagatt gacaataata aatacggcac
     2881 aattaatcaa aattaatttg atgatgtctg agccatattg atattgccat tttatgtctt
     2941 gccaaatagt catatctaat agaacgtagt tttatttgtt ttgttccaaa aaacaactaa
     3001 taaaaatccg aaaatagcac caccaatatg tgctgtatgt gcgatgttgc cgatgccagt
     3061 ttgtgcaaac atgatatcaa atacaatgat gacgattgga atcatatact tcgcttttat
     3121 aggcacaggc acaaacataa acattaaagg tgcatttgga aatagatagc cgaatgctgc
     3181 caacaaacca taaatagcac cgctagcacc cactaagctt gcgtaataaa tttgtgatag
     3241 acttttaatt gcagattcgt tcaatgtggc tggcaacgat gtttcattcg taaaaaagat
     3301 atcgccaatg tagtaatagg ccatacctgc attaattgcc aaatagaaag caattgcgcc
     3361 aagtccagaa aataaatata aaaaggcaaa tcgtttgctg cctaatactc gatcgctttc
     3421 cagcacagca ccaaaactat aaagtgcata catgttgaat aaaatatgga agatgccgcc
     3481 atgcgtaaac atgtgcgtca ccaattgcca aggtttaaat gctggatttg tcggataatg
     3541 catcgctaat atatctgtca aatcaggata tagaaaacag gctacgaata ctaaaatatt
     3601 tagtaagata aggtttttta ctacaggtgt taagttgttc attattttgt tttatcatgc
     3661 ttcgatacgc ttgttttttt gtctttgtca atgcgtgtca ttaataaata ttcttttgtc
     3721 gaagtaataa atgtaaatct aatttccaaa ttttctctca atttcatcaa aactatattg
     3781 cacaaaagta gccatgccta ctggattaat cattggctgc tcacaggcaa ataattcgtc
     3841 aatcaactgc tgcattgtgt ttttatcgag tgactttcct gattttattg ctgtttgata
     3901 agctaaggat tttgccacat ttttcctttt gtcaaattct tctgtcttgg cgccattctt
     3961 ataatcatct aacaattttt ctatcaaatg agtttcattt tcgtcacgca aatcggaagg
     4021 catgccgtga acgacaaatg tagtgcctcc aaaaaattcg atatcaaaac ctaagagttt
     4081 caagtcggac agcatctctt ttaataactc agcatcactg ttgttgaatt ctaaagattt
     4141 aggaaataaa gtttgctgtg tagaggaagg ctgtgaagca aaattgcgca aatatttttc
     4201 aaataaaatg cgctgatgtg ctttatgctg atcgattaaa atcaaaccac tttttatttg
     4261 tgtcaatata tatcgctcgt gtagttgtat cggctcgaag cgttcgtttt taaaaggatt
     4321 ggttgaaatg tcattttcat tttccaacgc caacaaatcc attttgcttt taatagttag
     4381 cgttttttct tccgtttgtt cgtcgatgtc gtataacttt ttccaatcat cgaaattagt
     4441 acctgtcgaa acagactttt gttgagtaag tggaatgtct ttgtcaaatg gtgaatgata
     4501 agaacctctt acattagagt cggaatttaa tttcgattgc actactttaa aaggctgctc
     4561 gttataaact ctttcatcta ccaataaatt gaaagtagct tcgtgttcaa agtcaattgt
     4621 tggcattacg ctgtactgcg acaagccatg ttttgccgcc gcattgagta agagataaat
     4681 ggctttctca tcttcaaact ttatttcctg ttttgttgga tgcacattaa tgtcaatggc
     4741 gctaggttct atgtcaataa acaaacaata aaatggaaaa tttttatctg ctatcatgcc
     4801 atcatatgcc ttgctaatag catgattcaa aaaattattt ttaatgaatc ggttattgac
     4861 aaagaaaaat tgttcgcctc gagttttttt gctcagctca ggtttgccaa taaaaccgcg
     4921 cacgctgaca tagtcagtga tttcttcaat aggcactaat ttctcgttgt aatttttgcc
     4981 aaacacattg acaatacgtt gtctgatatt gctcgacttt aaatgataga tttcactacc
     5041 attatgatgc aatgaaaaaa atacagatgg atttgctaag gcaacatgaa taaattcatc
     5101 aaggatatgc ttggtttcta tctgatttga tttcagaaaa ttacgacgtg ccggcacatt
     5161 ataaaataaa tgtcttaccg aaatggaagt gccagcatta gtagcaacgg gctcttgctg
     5221 tattactttt gagccttcga caatcagcaa ggtgccgacc tcctgctcgt gtaatttggt
     5281 tttcatttcc acttgcgcaa cagcggcaat acttgccatg gcttctccac ggaagccaaa
     5341 ggtataaagc gaaaataaat catctatttt ttttattttt gatgtagcat gtcgctcaaa
     5401 acacatacga gcatcggtct cactcatgcc gcagccatca tcaacaactt gtattaatgc
     5461 tctgccagca tcttttataa ttagctgtat atttttagcg ccagcgtcta tgctgttttc
     5521 caataattct ttcaccacag acgctggtcg ttgtatgact tcgccagcag caatctggtt
     5581 ggctatattg tctggtaata gttttatgat gtctgacata atacaagcac aaaatacaaa
     5641 atactactcg ttacaacgtg ttaaatgcta ttattttgat gcattaaatt agtataaaat
     5701 cttatttcca ctttcgttta aagattataa cacctaaaag actttatttt tagtatgttt
     5761 gacaaattag gtctgatttt tgttgtaggt ctgatttttg aattggaaat tataagatga
     5821 aaaataaaac atttacaata ttcttgttga ctatatgttg tatgttattt gctgcaaaca
     5881 caaaagcctc tgcaccaccc tatcttaagg atacgagtgc cgtttgggtt gattctattt
     5941 tgcagtcgat gtcattagat gagaaaattg gtcagttatt tatggtagca gcttattcta
     6001 ataaagacga agctagctat aaaaccatag ataaatatat caaagaatac aaagttggtg
     6061 gtttgatttt ttttcaagga acgccacaaa aacaagcaga actcacaaac agatatcaag
     6121 cgcaaagtaa aattccgtta tggataggtt ttgatggtga atggggtttg ggcatgcgtt
     6181 tagataacac gatttcttat ccaaggcagt tgacacttgg tgctatcgac aatccagatt
     6241 tgatatatga aatgggcgct gagattgcac gacaaatgaa acgtattggt attcatatca
     6301 attttgctcc tgatatagat gtgaatagca atccaaacaa tccagtgata aacgatagag
     6361 catttggcga taataaattt ggtgtcacac gtaaaggatt agcctatatg tttggtttgc
     6421 aggacaataa aattttagca agtggcaagc attttccggg tcatggtgat acagatgtcg
     6481 attcccataa agacttgccc atactaaaac acagcttaga gcgattagaa tcagtcgagc
     6541 tatatccatt caaacaattc attcgttatg gtattggcag cattatggtg tcgcatttga
     6601 atgtgctggc tttagatgca gcgcctaatt ttccagccac cttgtcacct aaaatcatac
     6661 aacaaaaact aatcgattct atgaaataca atggattgat ttttacagat gctatgaata
     6721 tgaaaggcgt agctaaattt tatccatcag gagtggctga agtaatggca ataatggcag
     6781 gtaatgacgt gatgctgttt ccagaagatg tggacaaagc tattgaaggc gtgcgctgtg
     6841 cagtcttgca agattctatt attagcgaag aaactatcaa tagaaaagtg cgtaagattt
     6901 tagctgcgaa atattggtta ggcttacaaa cctatcagcc tatacaacta gaaggcattg
     6961 atgcggatat caataatgat gcagcgaaat atctgaaatt taaattggta gaacatgcaa
     7021 taacagtagt aaaagattct gcacgattaa taccagtcgt aaatcttcaa aagcgaatag
     7081 cgaatgttag cattggcagt agcaaacctt ccgtgttttc taccacctta gataaatata
     7141 ctgcgattga tcatttttat ttaccaaagg aaacaacaga aactacattt aatgatgtgt
     7201 tgtctaaatt gaaagattat catttggtaa taattgatat tcaagatatg agccgttttg
     7261 ctgcaaaaaa ttttggtgta acagataatt cacgtttgtt tttaaataag ctcaacaaac
     7321 aaaatcaaac catacttact gttttaggtt cgccatacag cttgaaatat ttccaagatt
     7381 tcaacacaat aattgtaggc tataatgaag aagaagaagc cgaatctatt tgtgcacaaa
     7441 tcatatttgg cgcacgggct gctgaaggta ccttgccagt tacagttggt aatttcaaat
     7501 atggcgatgg tgaatgcaca caatcaaata tgcgcttgaa atatggcatt cctttagaag
     7561 taaatatgaa tgtccaaaaa ttgcaaaaag cagatgccat tatttataaa gcaatagacg
     7621 ataaaatttt tcctggcgct cagttattgg tcgcaaaaga tggtaatgtg atatacgaaa
     7681 aagcctatgg ttttcatact tatgacaata ccattgcaac aaaaataaca gacttatttg
     7741 atatcgcgtc cctgactaaa gttacagcta ctgtgcctat gattatgaag atgcaagaac
     7801 aaggcaaaat caacatcaat aaaaaattag gcgactactt tcactatcct gatggtgtaa
     7861 ataaaaaaga tatcaatatc aaagaaatgc tttgtcatca agcgggtttg caagcatgga
     7921 ttccatttta taaaaacaca ttagattctt taggaaatcc agatactttg tggtattcaa
     7981 aagtgcaaga ctccattttc tcagtaaaaa ttgttgatag tttatttttg aaaaaatcat
     8041 ttcaagattc tgtttggaat atgatatatc acagcgcatt atcgccagtg aaagaatata
     8101 aatacagcga tttaggctac tatttgttgc agcaaattat tgaaaaaaca tataaggatt
     8161 cattgcaaaa tattgtcaag agatatttat ttgcgccttt aggtatgaat tatacgcagt
     8221 acaatccgcg atggaatgct tatgatttga ataaaatcat gccgacggaa attgagaata
     8281 atttccgtaa atctttattg ttaggaaccg ttcacgatca aggtgcggcg ctgcttggtg
     8341 gcgttgctgg tcatgccggt gttttttcta atgccaacga tttagccaaa tattttcagc
     8401 ttttgctaaa tgaaggtacg tacggcgcta atcgatatta tgaagctgca acgattaata
     8461 aattcactac aaaacaatat ccaaataaca gaagagcaat aggctttgat aagccgacac
     8521 ctaaagggca tgacggccca acttgcgatg cagcatcgcc agaatcgttt ggacatacag
     8581 gctttacagg cacgatagct tgggccgacc caacaaataa tctcatttat atattcttat
     8641 gtaatcgagt gtatccaaat gcagcggtca ataatttagc aaaacaaaat attcgaccta
     8701 agatacaaca agctgtttat gatgctattg aaaaataaga tgttgtattt atgacttgaa
     8761 tttagttatg tgttgattta tatctttatt tttattaatt tgtacataaa ttaccactta
     8821 ctcattagaa ttaccactta cgcagtgtat gtttgaaatg agcgttaagt gtctaatttg
     8881 aaatgaaatg cagaactaat gaataacgtt tataacgaac taacagaatt gtttaccaaa
     8941 atagacaaag cattgtctat taaagatatt gatgaaatag cttcactcga taagttaaaa
     9001 gaaatatgcg attcctcaaa cgaaatgtct tctatttttg agatggatag catgtcctat
     9061 tcaatggttt attctaataa ggcagcattt gaatatttag gcattacgga agatacgata
     9121 caaaagatgg gttttaaata catcattagt ataattcatc ctgataatat ttcatcaatg
     9181 tataacatga ttaaatttta tgccaataaa gaaaataaaa atctttgtta tacaggcgtg
     9241 ttttttatta aagtaaaaaa tggttgggaa tggacatact cttgcatgaa accagcaata
     9301 tacaatgctg atggcactgt gaagtactta ctgtcaactg gttgtagcat agataattta
     9361 ttaaaatcac ataaaaacta caagaattta caaaacaatt tatccttctt tgaggcacat
     9421 gccgcaaaat atctttcgct cacaagcaga gaaaaagaag tattgcattt aattgcagaa
     9481 gaacatactt ctaaagaaat tgctggaatg cttcatataa gtccatatac agtagatacg
     9541 tatagaaaaa cactgattga gaaactacag gtaaaaagct ccataggttt agctcgatat
     9601 ggtgttttgt ttagcttaaa ataagctcgt ttatagcgtt ttatgccacc tatttaggct
     9661 caattccatg tttttttgtt aaatactcct aaatttaggt agttttgttg atgtatgtca
     9721 agaaaaatac actaaatgat aggattgtgc tgtaagaaaa taatgtagaa ctttacatca
     9781 tcactgaaac cataaactat gaaatgcatt ttatttcatt gcagcgcctt ctactccaaa
     9841 aactagaagg cgctgtcttt tatatgtttt tatagttgaa taattttagt cagaaagttg
     9901 atgttatatt gaaaatttat tacacttcgt aattaaaatt tacttcacag ccttcagagc
     9961 aatttttgca gatgtcaatt tcttttcgac cttttactaa ttgtgttctg aagtttttat
    10021 acaaatcatt attccaaatt tgtgaaaacg aatgcgtttg caaattgcct aattgatgtt
    10081 gtgcatcttt gtcaaaacaa caaggcacta cttttccgtc ccaagtaatg acacagctat
    10141 gccacaattt ccagcaagaa ttttctaagt tatttttaat agaatatgtg ccatcattat
    10201 tttttttata acgcgaatat tgttcatttt ctggtatcaa tggatttcca tttttatagt
    10261 catacacttg tgccgttttt aaaatcactt cgtccacgcc aatttctttc gctatttttt
    10321 ttaagtcatc aatctgatgt tcattaggtt ttaccactaa aaattgaaat ataatataag
    10381 gcgtttttgc tttcaattct tttttccatt ttaccatatt ttttgcacct tcaattactt
    10441 tatacaaatg gccgccaacg cgatagctct gatatgtctc ttgtgtagtg ccatcaatgg
    10501 aaataattaa tctatctaat ccactttcta ttgttctttt tgctacatca tctgttaaat
    10561 aatgagcatt agtagatgtt gctgtataga tatttttttg cttcgcatat ctgaccatat
    10621 ctaagaactt cgtatttaaa tagggctcgc cttgaaaata aaaggtcaaa tacagcaaat
    10681 gattggataa ttcgtcaatt gttttttcga aaaaagcagg cactaacatg ccaattggcc
    10741 gtgtgaaagc acgcaggcca cttggacatt ccggacagcg taaattacaa gatgtagtag
    10801 gttcaaatga tatcgaaaat ggcttgccgt attgaattgg ttttttaaaa tattttgtca
    10861 aataaaatga taaatatacc aaactcatat tccacacacg tcgaacagta agtttcgata
    10921 agaagttaat ggtgttttgt gtagaagcgt tcacaactca aaaatacaaa attaaaagtc
    10981 agaattgtta agtgttaaaa cgtattcgtt gcgccattta cttttattaa tttttgtcca
    11041 cgtatttata ttttgccaat attttttaca ataaatcgaa tcatttttca ttgttattct
    11101 ttttataaaa aatatcgaat cgaataccat actgttaccc atcttactaa aaagagttat
    11161 ttcagttttt gtgtttggaa taatcttaat aattgaatca ttttcttgaa ttgaatcatt
    11221 attcgtgaaa cttccagcaa cttttaaagt atcattcgtg taatttgaaa tagtaaagta
    11281 atcagtacta attgggtcac aagacgtagc aacaatagta ataaataata caataattaa
    11341 tttattcatt tgaaacccat ttatttttga attgcagttt tcattcctgt tttcggaatg
    11401 tgtttcttaa aatatcttat cataaaaaac agcaaaacaa cgatgacaat acaaacaatg
    11461 gcagcaataa caaatggaat aatgatactg aaaatactgc caacaatggc caaaatatgt
    11521 tctattgttg ctacaagcgg attggcaacg cccgccgtag tagccgtaga agtggcacgt
    11581 gtcaatgccg tgcctgcttg tatgatgcca gcactaccac cgccaacgat aatacctaag
    11641 ccccatttta acatcgggtc gatatcggga agtatggcag atgcagtgag taaagtgcca
    11701 gcaattacag atgctggcgt cattatagtg tccaaaatat tatcgataaa aggaatgtaa
    11761 tagcctccaa tttcaaaaat agttgcagcg ccaaaacaaa tcagtgcggc cgtgcttccc
    11821 atccatagga agccttcgcc taagtgcaaa atacccattt ttgtagcaat gcttgctgcc
    11881 aataatggca caaacactct aaatccacaa ctggcgctta agccaaggcc cattacaagg
    11941 ctcaacaaaa ctttattgtc gctaaaaaaa tgcaaagcat cttttgaaac aaattgcatg
    12001 atagttgtat ccatagttaa aaatagtatt ttttacagaa taacatcaaa gtatgcacca
    12061 aaattcccga cagatgttat aactagatta agaaatatct ttgtattgat gaatgttggt
    12121 atgtgttggt aaacttaata ttcgtgtata aatatcttct gacttattac atactgctta
    12181 ttactttatt tatttttgta aaaaatagta agatggaaat tagtggaaaa gtatttaaag
    12241 tattgcctgt agagtcaggt gaaggaaaaa atggaacatg gaaaaaacaa gctatcgtta
    12301 tcgaaacagc gcatgataga tttccgaaaa aagtggctgt tcagttttgg ggcgacttgg
    12361 tgaatcacga aaatttcgta gaaggaaatg atatttcagt agagtttgat gtagaaagcc
    12421 gtgagtatat gggaaaatgg tacactgatg taaaagcatg gcgtataaac acaaaatcaa
    12481 ctacacaaaa taatacatca agtgccaata cacaacaagc tgcacctgct catacaagtg
    12541 ctgatatacc agttgccaca ggtgctgatg acgatttgcc gttttaattt accaaggatt
    12601 tttttggtaa tatgaaacat aggcatctcc atcaataata atcataggat cgcccgtttt
    12661 tgggtgattt ttcttttgaa aggagagtgt ttgtgcgcct tcgttttgtg atacgatgct
    12721 aattgtatta ccatgtagtt tccatgaacc ttggtctgcg ttttggctgc tcgagcctcc
    12781 atagtagcca ccgccagagc cacttattga accttcgtat gtatattgat acgtgccatt
    12841 actattgata acaatacatc ttgtattgct tgagctggag gtatatgtag cggatgcatt
    12901 tgtccaacac catgtgccta ctattgattt atcaatagaa ccagattttg atgaattatt
    12961 tacagtgttt gtttttgcct tactaaatgt aacaggattt tcaaaaatac cacctgaaac
    13021 gaccaagttg ccgttggaaa atttataata ataattaaaa gttccatcat cggaaaatgc
    13081 gataatctta tttgccgtat gtgtaaatgt aaatgcgcca ccatctaaag agccattacc
    13141 attcgcttgt agtatcaatg tgcctactga aggattattc caagtgccaa ataatgtaga
    13201 ttgcgaaaac cctaaaactg gcaggataaa aattagcgat aaaaatatct ttctcatatg
    13261 tgtaatttat ggtcattcga agttatgaag atttttaaaa agttgccaca tcgcttttgt
    13321 gcttataatt gtgtgtatct aaaataaaac ttaatcaaat gtcaacaaac taattaaaac
    13381 aaaaatgaca tatttctatt taattagtga taaataaaaa tgataaatta gacttgaaaa
    13441 taatttgttc aataaaataa taagcatgac acatcaatat aaaatttctg gaatgagctg
    13501 taatggttgc agaaccaaag tagaaaatac actcaattct attcaaggtg taacggcaaa
    13561 ggtcacatta gagccaccaa tagcgacgat taccatggaa aaacatattc ctaccataga
    13621 attacaagag gcattaagtg ctgtagggaa atataccata gaaatgatgc atgatcaaca
    13681 tcatgatgct gatgaaacag aatcaacatc atgttgctca acgacaacgc acacggcaca
    13741 tcagcatgaa gtgccggatt cattaaaaaa caacgctggt aaatattatt gtcctatgca
    13801 atgcgaaggt gataaggtgt atgataaggc tggctcttgt cctgtttgtg gtatggattt
    13861 agtgcaaata ccaagcgctg atgataatga tgatagcact tataatgaac tttggaagaa
    13921 gatgaaagtt gccctactct tcacaattcc cattttctta attgcaatgt tggaaatggt
    13981 gcctgcctta gattttacag ccataatgcc tcaaatagct tggaattggt tgcagttttt
    14041 gttgtcattg cctgtcgtgt tttatgcttg ttggatgttt tttcaaagag catggacttc
    14101 cctaaagacc atgaatttaa acatgtttac cttaatcggc attggcgttg gagccgcatt
    14161 tttatttagt gtaattgcac tgctatttcc atctattttt ccagcgcaat tcaaagggca
    14221 caacggcact gtatatgtct atttcgaagc cgtaagtgta attctaacat tagtgttgtt
    14281 aggccaattg ttagaagcga gagcacatag ccaaaccaat ggagcaataa aggcactttt
    14341 gaaattagcg ccaactgagg caacattagt agaaaatggt gtagataaag tgattgccat
    14401 tgatgccata aaaaaaggtg acttattgcg tgttaaacca ggcgaaaaaa ttcccgttga
    14461 tggtataatc atagacggaa gtagtaatat agatgaatcg atgctgaccg gtgaaccaat
    14521 tcctgttgaa aaaaagataa atgacaaagt aagtgcaggc acgataaatg gcactaaatc
    14581 atttgtgata aaggcggaaa aaataggtgc agaaacattg ctgtcgcaaa tcatagaaat
    14641 ggtaaatgca gcaagtcgct cgagagcgcc aattcaaaaa ttagcagata aaatatcaaa
    14701 atattttgta ccaattgtgg tattaatttc tataatcaca tttattgttt gggcaatatt
    14761 tagtaaggaa tcgccgtatg tatttgcatt tgtaaatgcc attgctgttt taataattgc
    14821 atgtccttgc gcattaggtc tggccacacc aatgtcaata atggtaggcg ttggcaaagg
    14881 cgcacaaaat ggcgtattaa taaaaaatgc agaagcatta gaaaacatga ataaagtaaa
    14941 cgtgttgatt actgataaaa caggaacaat aacggaaggc aaaccatcag tggagaatat
    15001 catttcattt gataatgctg acacagataa attactcaga tttgcagcct cactcaatca
    15061 atatagcgag catccattag caacagcagt agtaaatttt gcaaaatcaa aaaatattac
    15121 tgtcattcct gttacaaatt ttgaggcaat tatgggtaaa ggtgttattg gaacaattga
    15181 aaacataaaa attgttgttg gtaataaaaa actaatggaa gatgagcaag tagttattac
    15241 agataaaatt gcatcgcaaa ttacacaaga acaaatcaaa gggaaaacgg tttctttcat
    15301 tgcagtaaat ggcgccgtgc aggcttatat taccatcact gatgctatta aatccagtag
    15361 taaacaagct ataaaagatt taatggacca aggtgtggaa gtcataatgc ttactggcga
    15421 caaccataat acggcgaaag ctgtagcaga tacattagga ttgaaacatt ttaaagcaga
    15481 atgcttgcca caagataaac taaacgaaat aaaacgattg caggcagaag gaaaaattgt
    15541 tgcaatggca ggcgatggta ttaatgatgc gccagcatta gcacaagcag atatcggtat
    15601 tgcaatgggc actggtactg atgtcgctat tgaaagttca gaaataactt tattacacgg
    15661 cgatttactg ggcatagtga aagcaaaaaa gctcagtcat gccgtgatga aaaatataaa
    15721 agaaaattta ttctttgctt ttttctataa tgccttagga attccagtag cagctggcgt
    15781 attatttcca ttttttggtc ttctattgtc gccaatgatt gcagcagctg caatgagttt
    15841 tagttcggta tctgtaattg ccaactcatt aagactgcga acaaaaaaaa tataaataat
    15901 gaaaaagaaa attattgcag tattgaagca gatagttttg tttctgaaaa aaatgttgaa
    15961 agcgatattc gttcggaaga aagaatgctg caagtaatga taaaagatta tctaaaaaaa
    16021 agaacgcctg tcatcctctg ataatcaagc gttcgtggtt tggcacctgt gatcccgctg
    16081 ggattcgaac ccaggaccca tacattaaaa gtgtattgct ctaccagctg agctacggaa
    16141 tcattgcttt tttacaaaag cgagtgcaaa gatagttttt tttctataaa tgtcaaaagt
    16201 atgtgcattt ttttctctac ttaacgctta tttaaacata atacctttga cagatagcgt
    16261 aaatttctgt tctgtcgtgc tgtcttgagc agcatctgct aatatcacca ctgattttcc
    16321 agggctattc gttggtactt taaataattt gtctttaaca tcaaaaagta tttcatcacc
    16381 ttctttatct ttaacagtaa accagcagcc ctcacttcta cagcttttat ctatttttcc
    16441 gctgatttgt atatcgttta ccaccggatt cgtatttatt ctggcaatta aatcgtcaac
    16501 tgtgattgtg tttttctcag aaaatttaac tcctaactgt ttgctagatg tttcacatgc
    16561 tgcaaatatg ctaagtagca gcacaatgat taatgacctt ttcattttat attattttat
    16621 ttcttcttta gaaatttgtc gtccattttc aaactgcaat ttacgtatta gtttgttatt
    16681 tacaagagag tcaaaaattt gcacttcacc atcaaacaca tcaatgtctt ccataatttc
    16741 cttttttgtg cccattgcgt atatattgcc attgtcatag tattctttat aaataccatt
    16801 tacatggttg tctttcatgc ttatttcttc tttaactttg ttttttggtg atttaaaata
    16861 atttttcacc acaccattca tcatattgtc tttgtaagtt ccttcttgtt gtaagcttcc
    16921 atcttcataa taaatagtaa atgcgccatc atatttattg tccacataat tctcagtttg
    16981 cattattttc ccatttggat gatacaatgt acgcttacca tttagcttgc catcagtaaa
    17041 atgcgactct tccagcattt tgccatcatc ataaaactta tgataaacac caatttttaa
    17101 ctcaggtttc tttttgtcga cgaaataact ttcgactact acgccttgcg catttttaac
    17161 ttctactttt tgtttgttgc tacatgcagt aaaaaatagt aacattgcaa aaacaataca
    17221 agtgctaaca tttaattgaa cattaacttt atacatcttt ctaaatattt aattttgaaa
    17281 caaaatacgg aatttttatg cacgaattac aacaaataat tgaaaacgca tggagtgata
    17341 gatcacttct gcaagaaaca gcaacactca cggctatcaa tgaagtgata acgttattag
    17401 atagtggaaa attgcgtgtt gcagcgccaa caacagaagg atggaaagta aatgattggg
    17461 taaaaaaagc tgttatctta tattttccta cacaaaaaat ggaaacaata gaggtcggcc
    17521 ctttcgagtt tcacgataaa attccattaa aaaccaatta tgcagcatta ggtgtgcgtg
    17581 tggtgccaca tgcaattgct cgccgtggtg cttacttaga aaaaggtgta attatgatgc
    17641 cttcttatgt aaatattgga gcttatgtag atagtggcac catggtggac acttgggcaa
    17701 cggttggcag ttgtgcgcaa attggaaaaa atgtacattt gagtggaggc gttggcattg
    17761 gtggcgtgtt agagcccgtg caagcagctc cagttattat cgaagatgat tgtttcatcg
    17821 gctcgagatg tatcgtggta gaaggcgtac gcgtggaaaa agaagcagtg ttgggcgcca
    17881 atgtggtgct tactatgtcc acaaaaataa tcgacgtgtc tggtagcgaa ccaaaagaat
    17941 atagaggagt ggtgcccgca agaagcgtgg tcattccagg aacaattcca aaagaatttc
    18001 cg
//
LOCUS       DAOQQV010000046        17931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_966_length_17931_cov_8.871839, whole genome shotgun sequence.
ACCESSION   DAOQQV010000046 DAOQQV010000000
VERSION     DAOQQV010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17931
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_17931_cov_8.871839"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..2892)
                     /locus_tag="PLL59_06370"
     CDS             complement(106..2892)
                     /locus_tag="PLL59_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY23466.1"
                     /translation="MNAFLKHTLTIFSIVIPMLTWAQTGKISGIIIDKNTQETLIGVN
                     VSIDSSMLGATTDIDGKYEITDLTPGQYNFIISYIGYANKYIQNIIVRADEVTSLDIH
                     LEVASNELAEVQVVDFKKTNTETAVLLEMKNANLIASGISSQQIQKSADKDAAQVVRR
                     IPGVSILGNFINIRGLNQRYNNVLLHNAMAPSMETDVKSFSFDIIPSAQLDRIMVVKS
                     PSADIVGEFAGGMVKIFTKSFPDNNFVDVSYGTSFRVGTTFKSFYNQKNSALFYLAND
                     PQNNMASNFPKNLNSVQQQYLKPIAESMNNDWSPTKKTAIPDQKIGISFGRRISTDKL
                     LLGMITSINYSISKQTNTIFRADYNLYDFANDKQAPKYNFTDEQYNNNVRLGILHNWA
                     FKFKKNTIEFKNIYNATALSQYTHRYGVDFANDLSLDNHALYNTYKGIYAGQLLGKHQ
                     LHTEHNTMDWVFGYSRAYKNEPDFRRYTSTLNTTSNVYEINVLPTAVNANNLGRFFSK
                     MHENIFTLGLNSQNKIVKRIDKGFIPSINTGAYIEYKNRDFNARVLGFKRADLDMFDY
                     SLTTQGIDYLLQHINSTNGIKLAEQTRSYDSYAANNLIAAAYVNTEFAIKSKVRIVAG
                     LRYEYSNQQLTSGKSTDLVKIRNQKNAYLPSLNISYTITPKMVLRAAYGMSVNRPEFR
                     EIAPFAFDDFYTRYTIEGNPSLKNASVHNSDLKFEYYPSNGEVISVSVFYKKFINPIE
                     SKAVVGTSGSTFSFANAYQSDVAGVELEIKKSFTTAKNYFFQHFGLMANASYIYSKVN
                     LGKENIGQSNNRPLQGQSPYTVNAGVFFEDKELGLQANLLYNVIGKRIAFVGTDDNPD
                     IYEMPRHTLDFNIQYRFKKNVEISLSANDLVNQSVLFIQDGNRDKKWNRKSDQIFQEY
                     QPGQSISLGVKYNF"
     gene            3093..5885
                     /locus_tag="PLL59_06375"
     CDS             3093..5885
                     /locus_tag="PLL59_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HMY23467.1"
                     /translation="MIAFLEKITESISKKDLPYLYKRCYIFPSRRAAIHFSNFLKEKF
                     KNDNFIFPEIITIQNFIQSNTSFNVKDDWYLLSKLHQTQNALTHTNQAFEAFLPWGKL
                     ILKDFDECDKYLVDATQLYSILKAHKALEDGAVISDEIRQYIEQFIATTTKKEKEGIF
                     KDSFIKTWSLLGDMYQHFQEELSQKQFAYEGMAYRDVLQKLQNNTLQLPYSKIACCGF
                     NALSRCEEEIFKTLSQQYDTEFWWDADVLFIDNKFHEAGNLLRRYQQIFPTENGNSHW
                     IIDDELTQNKNITISGISSNIGQAMYVAQHALQHQQEKTAIVLCDENLITPLLSTVDT
                     TQANITMGYPIAQSELYSLLKLLLNFYGNARVTENKIAFYHKDISALIEHHYFNNFVT
                     NKEKLHTQLAYFVPFMPQEEIQQYISIDLLSVHNSSSTALSYILSTLRKVESKDAYFA
                     AIKHTLIEQIQNIIHLLLENNINIQRNALSFFVQQYISNCKVSFDTNTKSNTQIMGFL
                     ETRLLDFDNLYILSMNDDQLPGTNKTNSFIPYNLRKGFGLPTFEQFDGVNAYHFYRLL
                     KRAKNIQLLYNNQVGDNASEKSRFIRQLTYDMATPKNTVKEQIVVLEKLGNSPTVTSS
                     LLQIKKTDEIIANLRERKFSPSAIKTYIKCPVQFYMKYVAGIDEPSEIEEEIDASVFG
                     LILHKTLELIYLPYLNAEISAKTILSFTDKDFIKEKIKVTCNELELPKEITQGSNKLQ
                     LRIIERIVEKILHNDAANKQLTILFAEQKFIWQHLALTDGTFASIQGTFDRIDKVGDH
                     GIRIIDYKTGKIELPAFPDMSKDEKIEEFLDTLFLFDKKDYSATFQGIMYALIYYKLY
                     HCTEIYVGYHHAKAMKDGIVYLNNQEAIPTALLLSFEKRLSALLSELIYQAPYFKQSE
                     NEEAYKYSAYASFINI"
     gene            complement(5894..6385)
                     /locus_tag="PLL59_06380"
     CDS             complement(5894..6385)
                     /locus_tag="PLL59_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23468.1"
                     /translation="MEKKLFLSIVYEIIWFLLAIGGAYICIVPIQDEISKPFFQFLLF
                     ALFIAFTYFRFIAFMMRSILLESIWAKLLLFVLNVPLFFLLLNKYYAFGRTFDDYVFT
                     LPANVFQHIKSGTDIDDLMYIKNLVTFAGVTSMTVIVLLEMRIVYAIFKLRQLDKILY
                     KKK"
     gene            6529..7236
                     /locus_tag="PLL59_06385"
     CDS             6529..7236
                     /locus_tag="PLL59_06385"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HMY23469.1"
                     /translation="MKLITTTKQIAVLIDPDKTNLKKLSTLIVSANKANIDFFLVGGS
                     LLQKNNLEEIVVYLKKHSKKKVILFPGNPTQICAKADGILLLSLISGRNADLLIGNHV
                     QAAPLLKQSKLDIIPTGYILIESGTATTVSYISNTTPIPANKPEIAVCTALAGEQLGL
                     QCIYLEAGSGAKNAVHQETIRAVRKSISLPLIVGGGLRSKAQIEAAFDAGADIVVVGT
                     AIENNIHFLDEIKSIKK"
     gene            7273..8202
                     /gene="rsgA"
                     /locus_tag="PLL59_06390"
     CDS             7273..8202
                     /gene="rsgA"
                     /locus_tag="PLL59_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMY23470.1"
                     /translation="MKGKIIQTTGSWYQVLVDGKIIAARLKGKFKLDQFDTTNPIAVG
                     DDVIIEATERHEYVIQEILERHNYIIRQSPRNKHQRHIIASNIDLALLIVTISQPRTS
                     LGFIDRFIAAAASFHIPVKIIINKIDLIKEKEMEQLEYMQIVYNRLGYDVICSSTITN
                     EGIEQIRAVLANKTTLVAGHSGVGKSTLLNVLQPELNLKTGKISEKWGKGMHTTTFAT
                     MFQLFENTFIIDTPGIKEFMMLQVEPEEVSGYFIDIKQFATNCQFNNCLHQNEPNCSV
                     KDAVENKQIAATRYENYLHIIENIKDINYWERK"
     gene            8434..8973
                     /locus_tag="PLL59_06395"
     CDS             8434..8973
                     /locus_tag="PLL59_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23471.1"
                     /translation="MINYCIDNTIIENDNYLYIGEEYFRCVRKYFILLDIPLEKVHKL
                     FEKHDNMLQLLSSIISVYQLDESLLHSCCILINVQYNLKDIKQIENLTKVRNRNKYYE
                     ILKPDFALHFKQQILTHNIPQYYDSSIIVMQKFNSTLLYISSKLKHIFVAHEMLYVEL
                     LLTIDGQLNKKIIHYLNTL"
     gene            complement(8981..9259)
                     /locus_tag="PLL59_06400"
     CDS             complement(8981..9259)
                     /locus_tag="PLL59_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23472.1"
                     /translation="MKKSYSTCIIPQTSEGLLFSLQCDDYLYHCWASNQQFVLIKESN
                     NFALCEPNQLLNFTERELKRYLFNATKQLKELDNILFFNELLWCKYCE"
     gene            complement(9371..10267)
                     /locus_tag="PLL59_06405"
     CDS             complement(9371..10267)
                     /locus_tag="PLL59_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HMY23473.1"
                     /translation="MSLTGKLKRFDLIINKVERTKYPSLQVILKYLENYGHTVTARTL
                     QRDLEELKSEFKIFIEYSSFNRGYFIEKDSMSDNVIQFIKNMTLQANLLDFAKLGEHP
                     NAIILKENHALKGVEFIPQILTAIQQQLQIDFSYQAFEKDKPKNYTVQAYALKEYLGR
                     WYMVACSTSRKGIVKFGVDRILSLTITEVKFKKDKQIDLSAYFSRMIGIIDEGAACEQ
                     VILSFTPMQAKYIRTLPWHWSQEEVLTNDKEVHFEYFILTNYELMQKILSYGNTVTVV
                     KPATLKKEHQRYLKEALAQYKK"
     gene            10350..10802
                     /gene="dtd"
                     /locus_tag="PLL59_06410"
     CDS             10350..10802
                     /gene="dtd"
                     /locus_tag="PLL59_06410"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HMY23474.1"
                     /translation="MRVVIQRVANASVSIEGIEKGKINTGLLIFLGIEEQDDAADIAY
                     LVKKIIPLRIFSDEAEKMNLSIQDIHGDILLISQFTLHASTKKGNRPSFIRAAKPASA
                     IPLYEQFIKEMELQLGKKICTGIFGADMKITLLNDGPVTIIIDSKNPE"
     gene            11173..12150
                     /locus_tag="PLL59_06415"
     CDS             11173..12150
                     /locus_tag="PLL59_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HMY23475.1"
                     /translation="MNTALATEKILQESDNRFVLFPIKHDAIWAMYKKHLAAHWVTEE
                     VDLSQDIIDWNNKLNDNERHFIKHVLAFFAASDGIVNENLVVNFMQDVTIPEVRCFYG
                     FQVAIENIHAEMYSLLIDTFIRDTAEKDKMFNAIETLDCVKLKASWALKWIETAPSFA
                     HRLVAFAAVEGIFFSGSFCSIYWLTDRGLMKGLGKSNEFISRDEGMHCDFACLLYSML
                     ENRLSKEEIYQIICDAVECEKVFVKDSLPVSLIGMNEKLMCEYIEFCADRLIVALGYP
                     KVYNAQNPFPFMEKISTQGKTNFFEERPTEYVKAGVESGEKKFETDDDF"
     gene            12206..14719
                     /locus_tag="PLL59_06420"
     CDS             12206..14719
                     /locus_tag="PLL59_06420"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HMY23476.1"
                     /translation="MYVIKRDGRKEPVHFDKITARISKLSFGLNATFVVPHRVAQAVI
                     QGLYDGVTTTELDELAARTSANMTVVHPEYAVLAARIAVSNLHKNTKKNFSRIIEELY
                     NYIDPKTGKQAPLIADDVYEIIMTNKDKLDAAIIHHRDYNYDFFGFKTLEKSYLLKMH
                     GKVVERPQHMLMRVAVGIHKNDIDAAIETYDLMSDKWFTHATPTLYNAGTPKAQLSSC
                     FLLTMKDDSIKGIYDTLKNCAEISQAAGGIGLSIHNVRAKGSYIKGTGGESNGLVPML
                     QVFNSTARYVDQGGGKRKGAFAIYLEPWHADVFDFLELRRPHGKEEMKARDLFFALWI
                     PDLFMKRVKENGTWSLMCPNECPGLADTYGDEFEALYERYEAEGKFRKQIKAQELWFH
                     ILDSQTETGTPYMLYKDHVNRKSNQKNLGVIRSSNLCTEIVEYTSADEIAVCNLASIN
                     LSRFVENNTFNFQKLYDITYIVTKNLNKVIDVNFYPVQEAQNSNMRHRPVGLGVQGLA
                     DTFMMLRHPFDSEEARILNKNIFETIYFAALSASKDIAKVDGPYETFKGSPISEGKFQ
                     FDLWNVTPTNRWDWDTLRSEVMQHGVRNSLLVAPMPTASTSQILGNNECFEPYTSNIY
                     TRRVLSGQFMVVNKHLLKDLIDLGLWSEEMKNAIIANNGSIQSIEGIPQETKELYKTA
                     WEIKQRAIIDMAADRGAFIDQSQSLNLFMESPNYKKLTSAHFYAWEKGLKTGMYYLRS
                     RPAVDPIKFTVDVEKAKMSNATAQQQDTNSDKVASIAPIYESVANAQQEMFVVTEPTI
                     QEKSIEEKAAEFGMSVDEFLSAQKVCSLENPGECEMCGS"
     gene            14818..15375
                     /locus_tag="PLL59_06425"
     CDS             14818..15375
                     /locus_tag="PLL59_06425"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HMY23477.1"
                     /translation="MSFLQQPFAWYIAGPLIALNLFLLLYFGQRFGISTGLKTTCAAL
                     GAGSISQFFKYNWKEEAWLLVYIVGTIIGGFIAFTFMNAGDAVIVSDNTKAALENMNI
                     VANQTFAPDEIFSIENLFTLKGSSILVIGGLLIGFGTRWANGCTSGHAISGLSDLQLP
                     SLVAVIGFFIGGLLMTFLFIPLIFK"
     gene            15377..15790
                     /locus_tag="PLL59_06430"
     CDS             15377..15790
                     /locus_tag="PLL59_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HMY23478.1"
                     /translation="MRLLKFLLLGIFFGIVLAKAQVISWFRIYEMFRFESFHMYGIIG
                     SAVILGVLIIQLIKRKKLRAFNGEAISIAPKPNTYKASIFGGILFGLGWALVGACPGP
                     IFIMIGAGFWSFIVVLAGALAGTFIYGLIKEKLPH"
     gene            15852..16169
                     /locus_tag="PLL59_06435"
     CDS             15852..16169
                     /locus_tag="PLL59_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMY23479.1"
                     /translation="MAHEDLAPKEFLAAYNEQKDNAVILDVRTAAEIADANIDGHIAI
                     DIQQPGFMEKVAELDKSKTYFVYCRSGNRSGQACRYMSTQGFEKLYNLKGGMMAWMET
                     DFE"
     gene            16353..16835
                     /gene="eco"
                     /locus_tag="PLL59_06440"
     CDS             16353..16835
                     /gene="eco"
                     /locus_tag="PLL59_06440"
                     /inference="COORDINATES: protein motif:HMM:NF002987.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HMY23480.1"
                     /translation="MKHIIILFALLIAATTTPLQAKNKKSKLATIDISMFPKAKPGYK
                     MLYIQVPATKQENDLKVEFFVGRNEQVDCNKYFLTGDIKEQNLEGWGYTYYTVESDGN
                     IAGTKMACPDNVLQTKFVHLQGEITRYNSKLPIVLYVPEKLDVQYRIWKADKKMQHAK
                     "
     gene            16903..17265
                     /locus_tag="PLL59_06445"
     CDS             16903..17265
                     /locus_tag="PLL59_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23481.1"
                     /translation="MKKYILCSIFIPSMLFGQHKLKVKPMDSLAVLQFAKERKWIFQQ
                     HDDMSETAPVYAPIISYDTTKKQWTIVSKRYTTTRKGKCKHTNGCTIEHTQIIIIDAI
                     NKRLIKRQINKKAYPNYE"
BASE COUNT         6257 a         3053 c         2974 g         5647 t
ORIGIN      
        1 atagtcaatg ctacaaatac aaagaagtaa tgttgtttgt tgtttttaaa tgttaatgtc
       61 ataaaatttg ttttgtttgt ttatttactt ggcttttgcc attttttaaa aattatattt
      121 tacacctaaa gaaatggatt gtcctggttg atattcttga aaaatttggt ctgattttct
      181 attccatttt ttatctctat tgccatcttg aataaatagc acagattgat taactaaatc
      241 atttgcggaa aggctgattt caacattttt cttaaagcga tattgtatat taaaatcgag
      301 tgtatgcctt ggcatttcat agatatcagg attgtcgtcg gttccaacaa atgcgatacg
      361 tttaccgata acattgtata gtaaatttgc ttgcaaaccc aattctttat cttcaaagaa
      421 aacaccagca tttacagtgt aaggtgattg accttgtaat ggtcgattat tgctctgccc
      481 tatattttct tttccaagat ttactttgct atagatatat gaagcatttg ccattaaacc
      541 gaaatgttgg aagaagtaat ttttagcagt agtgaatgat ttctttattt ctaattctac
      601 gccagcaaca tctgattggt atgcattcgc gaaggagaat gtactaccac ttgttcctac
      661 tactgcttta gactctattg gattaataaa ttttttataa aaaacagaaa cactaattac
      721 ttcgccatta gaaggataat attcaaactt caaatcgctg ttgtgtacgg aagcattttt
      781 caaagatgga ttgccttcaa ttgtatatcg cgtataaaaa tcatcaaaag caaaaggtgc
      841 gatttctcta aattcaggac gatttacgct cataccgtaa gcagcacgca ataccatttt
      901 aggcgtaata gtatagctga tatttaatga aggtaagtag gcattttttt gatttcttat
      961 tttcaccaaa tcagttgact tgccagatgt caattgctgg tttgaatact catagcgcaa
     1021 gcctgcaacg atgcgcactt tgcttttaat agcaaattct gtatttacat acgcagcagc
     1081 aattagattg tttgctgcat aggaatcgta tgagcgagtt tgctctgcta atttaattcc
     1141 atttgtgcta ttaatatgtt gtaataaata atcaattcct tgtgtagtaa ggctataatc
     1201 gaacatatct aaatctgcac gcttaaaccc taaaactctt gcattaaaat ctctgttttt
     1261 atactctata tatgctccag tattgattga tggtataaaa cctttatcta tacgttttac
     1321 tattttattt tgcgaattta aaccaagcgt aaatatgttt tcatgcatct tagagaagaa
     1381 tctgcccaaa ttattagcat taacagcagt tggcaataca ttaatctcat acacattaga
     1441 tgtagtgttt aatgttgaag tgtatcttct gaaatctggt tcatttttat aagctctact
     1501 atagccaaaa acccaatcca tcgtattgtg ttctgtatgt aattgatgtt ttcctaataa
     1561 ttggcctgca taaattcctt tataagtatt atataatgca tgattatcta gacttaaatc
     1621 attagcaaaa tcgactccat atctgtgtgt atattgtgat aatgctgttg cattgtaaat
     1681 gtttttaaat tcaattgtat ttttcttaaa cttaaacgcc caattatgta atatgcctag
     1741 tcgcacattg ttgttgtatt gttcgtctgt gaaattatat ttaggagctt gtttatcgtt
     1801 tgcaaaatcg tacaaattat aatccgctct aaagatggta tttgtttgtt tagaaatact
     1861 gtaattaata gaagtaatca tacctagcaa caatttatct gtagaaattc gtctgccaaa
     1921 actgatacca attttttgat caggaatagc tgttttcttt gtaggcgacc aatcattatt
     1981 catactttca gcaattggct ttagatattg ctgctgcaca ctattcaaat ttttaggaaa
     2041 atttgatgcc atattatttt gcggatcatt ggctaaataa aacaaggcac tatttttttg
     2101 attgtaaaat gatttaaatg tagtaccgac tcggaatgac gtaccataag aaacatcaac
     2161 aaaattattg tctggaaaac ttttcgtaaa aatcttcacc ataccacctg caaattcgcc
     2221 aactatatca gcagatgggc ttttgacaac cattattctg tctaattgtg ccgacggaat
     2281 aatatcgaat gaaaatgatt ttacgtcagt ttccattgat ggtgccatag cattgtgcaa
     2341 cagcacatta ttatatcgtt gatttaaacc acgtatatta atgaaattgc ctaaaatact
     2401 tacgccagga atacgtcgca ctacttgtgc tgcatctttg tctgctgatt tctgaatctg
     2461 ttgcgatgaa attccactcg caattaaatt cgcatttttc atttccagta agacagctgt
     2521 ttctgtattt gtttttttga aatcgacaac ttgtacttct gcaagttcgt tgctggcaac
     2581 ttctaagtgt atgtctaatg aggttacttc gtcagcgcgc acgattatgt tttgtatata
     2641 cttatttgca taaccgatgt aagaaataat gaaattgtat tgacctggcg tcaagtctgt
     2701 gatttcatat ttgccatcta tatctgttgt agcaccaagc attgagctat cgattgacac
     2761 atttacacca attagtgttt cttgtgtatt tttatcgata ataattcctg aaatttttcc
     2821 tgtttgtgcc catgtcaaca ttggtattac aatgctgaaa atggtaagtg tgtgttttaa
     2881 aaatgcgttc atttgtgtga ttaatttgat actgcaaagt aaaaacagca gtttcaccac
     2941 caagtaaagg gcgattgaat gttttatggt ttattggcaa ttaatacgtc tttttagtac
     3001 gatataagga attattagag gcaattgtgt gaacataaag tgaacgattg taaggaaata
     3061 gctttactat atagttttcg tatcttagta aaatgatagc atttttagaa aaaattactg
     3121 agtccatttc gaaaaaagat ttgccgtatc tttacaaacg ctgttatatt tttccatctc
     3181 gcagggcagc tattcatttt agcaatttct tgaaagaaaa atttaagaat gacaatttta
     3241 tttttccgga aattattacc attcaaaatt ttattcaatc aaatacttct ttcaatgtaa
     3301 aagatgattg gtatttattg agcaaattac atcaaacaca aaatgctctt acacatacga
     3361 atcaagcctt tgaagcattc ttaccttggg gaaaattgat attaaaagat tttgatgaat
     3421 gcgataaata tttagtagat gccacacaat tatattccat tttaaaggct cataaagcat
     3481 tagaagatgg tgctgtaatt tcagatgaaa tccgtcagta tatcgaacaa ttcattgcca
     3541 caactacaaa aaaagaaaaa gaaggcatct tcaaagactc atttataaaa acatggagtt
     3601 tgttaggcga tatgtatcag cattttcagg aagaattatc acaaaagcaa tttgcttacg
     3661 aaggaatggc gtatcgagat gtattgcaga aactacaaaa caatacacta caattaccct
     3721 attccaaaat tgcatgctgt ggttttaatg cgctatcacg ttgcgaagaa gaaatattta
     3781 aaacactttc acaacagtat gatacagaat tttggtggga cgcagatgta ctttttatcg
     3841 acaataaatt tcatgaagca ggaaatttat tgagaagata tcagcaaata tttcctacag
     3901 aaaacggaaa tagccattgg attattgacg acgaattgac gcaaaataaa aacattacca
     3961 ttagcggtat ttcttctaac attggacaag cgatgtatgt agcacaacat gccttgcaac
     4021 atcaacaaga aaaaacggcc attgtattgt gcgatgaaaa tttaatcaca ccattgctta
     4081 gtaccgttga taccacgcag gcaaacatca ctatgggtta tcctattgcg caaagcgagt
     4141 tgtattcact tttgaaattg ctattaaatt tttatggaaa tgcacgtgtt actgaaaata
     4201 aaattgcatt ttatcataaa gatattagcg cattaattga acaccattac ttcaataatt
     4261 ttgttactaa taaagaaaaa ttacacacac agcttgccta ctttgtgcct tttatgccac
     4321 aagaagaaat acagcaatat atctctattg atttactaag cgtacataat agcagcagca
     4381 ctgcgctttc atatattcta tctacacttc gtaaagtcga aagcaaagat gcttattttg
     4441 ctgctataaa acatacatta atcgaacaaa tacaaaacat catacatctt ctattagaaa
     4501 ataacattaa catacaacgc aatgctttat cgttttttgt gcagcaatat attagcaatt
     4561 gcaaagtatc tttcgataca aacactaaaa gcaatacgca gataatggga tttttagaaa
     4621 ccagattact agactttgac aatctctata tactttccat gaatgatgat caactgcctg
     4681 gcaccaataa aaccaattct ttcattcctt ataacttgcg aaaaggattc gggctgccta
     4741 catttgagca atttgacggt gtgaatgcat atcattttta tcgcttacta aaacgtgcga
     4801 aaaatatcca gctattgtat aacaatcaag tgggcgacaa tgcaagtgaa aaaagtcgat
     4861 tcatcagaca gttgacatat gatatggcaa cgccaaagaa cacggtaaaa gaacaaatag
     4921 tagtattaga aaaacttggc aacagtccta ctgtcacatc aagtttatta caaattaaaa
     4981 aaacagatga aatcatcgcc aatttaagag agcgaaagtt ttcaccctca gcaataaaaa
     5041 cgtatatcaa atgtcctgta caattttata tgaaatatgt tgccggcatt gatgaaccga
     5101 gtgaaataga agaagaaatc gatgcctctg tatttggttt aatattacac aaaacattag
     5161 aactcattta tcttccatat ctgaatgccg aaatttcagc aaagacaatc ctatcattta
     5221 ctgacaaaga ttttattaaa gaaaaaataa aagttacgtg taatgaatta gaattaccta
     5281 aagaaataac acaaggcagc aacaaattgc aattaagaat catagagcgc atcgttgaaa
     5341 aaatattgca caatgatgca gcaaacaagc agcttacgat tttatttgca gagcaaaaat
     5401 ttatatggca acatctagca cttactgatg gtacatttgc cagtattcaa ggtaccttcg
     5461 acagaataga caaggtaggt gatcatggca ttcgcatcat cgattataag actggaaaaa
     5521 tcgaattgcc agcattccct gatatgagca aagatgaaaa aattgaagaa tttttagata
     5581 ctttattcct atttgacaaa aaagattaca gcgccacatt tcaaggaatt atgtatgcat
     5641 taatatatta caaactatat cattgtactg aaatttatgt cggctaccat catgcaaaag
     5701 ccatgaaaga tggtattgtt tatttaaaca atcaagaagc aattccaaca gccttattat
     5761 taagctttga aaaaagatta tctgcgttat taagtgaact catttatcaa gctccatatt
     5821 tcaagcaatc tgaaaatgaa gaagcctata aatactctgc ttatgcgtct ttcatcaata
     5881 tataggaaag tgattatttc tttttataaa gaattttatc caattgtcgt aatttaaaaa
     5941 ttgcatagac aatacgcatt tccagtaaca cgattaccgt catggacgta acgcctgcaa
     6001 atgtcaccaa gtttttgatg tacattaaat catcaatatc agtacctgat tttatatgct
     6061 gaaaaacgtt agcaggaagt gtaaaaacat aatcatcaaa tgtacgtccg aatgcataat
     6121 atttatttag caataaaaaa aacaaaggca cattcagtac aaacaacaac agttttgccc
     6181 aaatactttc caacaatatc gaacgcatca tgaatgcaat aaatcgaaaa taggtaaatg
     6241 ctataaacaa tgcgaaaaga agaaactgaa aaaatggctt actgatttca tcttgaatcg
     6301 gcacaataca aatatatgca ccaccgatgg ctagtaaaaa ccaaataatt tcgtaaacga
     6361 tacttagaaa cagcttcttt tccatagcgc aaaggtacac aaccaaaatc taattcttat
     6421 tagataaacg ttattagata atgcgtttgt cgccaataaa cgcctattca agcgcataat
     6481 atgtagtgtc aatagtaatg tttgacgaat ttttatattt ttacagtaat gaagctaata
     6541 actacaacaa aacagattgc tgttttaatc gatcctgaca aaacaaatct aaaaaagttg
     6601 agtacactaa ttgtgagtgc taacaaagct aatattgatt tttttttggt aggcggcagt
     6661 ttattacaaa aaaataatct ggaagaaatc gtagtttatt taaaaaaaca cagcaagaaa
     6721 aaggtcatct tatttccagg aaatcctact caaatttgcg caaaagcaga tggcatttta
     6781 ctactaagtt taatctctgg cagaaatgcc gatttattaa ttggcaacca tgtacaagct
     6841 gctcctttat taaaacaaag caaattagac attattccta caggatatat tttaatcgaa
     6901 agtggaacag cgacaacggt ttcttacatt tctaacacca caccaattcc tgccaacaag
     6961 ccagaaattg ctgtatgcac agcacttgca ggcgagcaac ttggtttaca atgcatctat
     7021 ttagaagcag gcagcggtgc taaaaatgct gttcatcaag aaaccattcg cgctgtaaga
     7081 aaatcaattt cacttccact tattgttggc ggcggactac gcagtaaagc acaaatcgaa
     7141 gcggcttttg atgctggtgc agatatcgta gtggttggta ctgctataga aaataacatt
     7201 cattttttag atgaaattaa aagtataaaa aaataatttc gatcaaaata tatcttccat
     7261 aacaaaatcg tcttgaaagg caaaatcata caaacaacag gctcgtggta tcaagtattg
     7321 gtagatggaa aaattatagc tgcacgctta aaaggaaaat ttaaactcga ccagttcgac
     7381 actaccaatc cgattgctgt tggcgatgat gttattattg aagctacgga aaggcacgaa
     7441 tacgtcattc aagaaattct agagcgtcac aactacatca ttcggcagtc gcctcgcaac
     7501 aaacaccaac gacatattat tgcctcaaat atcgacttgg cattgctcat tgtaaccata
     7561 tctcaaccac gcacatcatt aggttttatt gatagattta ttgccgccgc tgcatcattt
     7621 cacattccag taaaaatcat catcaataaa atcgatttga taaaagaaaa agaaatggag
     7681 caattggaat acatgcaaat cgtctataat cgtttaggtt acgatgttat ttgcagttcc
     7741 accatcacca atgaaggcat tgaacaaata cgtgctgtat tggcaaacaa aacgacatta
     7801 gttgctggac attctggcgt tggcaaatca actttattaa atgttttaca gcctgaatta
     7861 aatttaaaga ctgggaaaat atctgaaaaa tggggaaaag gcatgcatac tacaacgttt
     7921 gctaccatgt tccaactttt tgaaaacaca tttattatcg acacaccagg catcaaagaa
     7981 tttatgatgt tacaagtcga accggaagaa gtttctggct attttatcga catcaaacaa
     8041 ttcgctacta attgccaatt caataattgt ctccaccaaa acgaaccaaa ctgctcggtg
     8101 aaagatgctg tagaaaataa gcaaattgcc gctacgcgct acgaaaatta tttgcacatt
     8161 attgaaaata tcaaagatat taattactgg gaaagaaaat aacttcctgt cattttcata
     8221 ctacactgta ttttttatgg gcacattaat acatgtgtat tcttgaggtt tgtcgtttta
     8281 aaaatgattt ttgatgacat ttattaattt aatttattta ctacgacatg ttttgtcgta
     8341 gttcagcatt aattttgtta tacaaaaacc atattacttt tcggctggca ttctgccagc
     8401 cgaaaaaccc aaatttatta actatttagg tttatgataa actattgcat cgacaataca
     8461 ataatagaaa acgacaacta cttatacatt ggagaagaat acttcagatg cgttcgaaaa
     8521 tattttatcc tcttagatat tccactagag aaagtacaca aactatttga aaaacatgac
     8581 aacatgttgc aattactcag tagtattatt agtgtttatc aactcgatga aagtttattg
     8641 catagttgtt gtattttgat aaatgttcaa tacaatttaa aagacatcaa acagatagaa
     8701 aatttgacga aagtaagaaa tagaaataaa tattatgaaa tattgaaacc agattttgca
     8761 ctacatttta aacagcaaat attgacccat aacataccgc aatactatga ttcatcaata
     8821 attgtaatgc aaaagtttaa ttccacgtta ttatatattt caagtaaatt aaaacacata
     8881 tttgtagcac acgaaatgct gtatgtcgag ttattattga ccattgacgg acagcttaac
     8941 aagaaaataa tacattacct gaacacgcta tgatctttta ttattcacaa tatttgcacc
     9001 ataacagttc attaaaaaac aaaatattat ctagttcttt gagctgctta gttgcgttaa
     9061 ataaataacg tttcaattca cgttctgtaa agttcaacag ttgattcggc tcacacaaag
     9121 caaaattatt agactcttta atcaacacaa actgctgatt gcttgcccag caatggtata
     9181 aataatcatc acattgcaaa gaaaacagta agccttctga tgtttgtgga ataatgcagg
     9241 tagaatacga ttttttcata ttccaaagct aaatcacagg tacgacacaa tatgtcgtag
     9301 taaaaaaaca attttatatt tattgtaaaa aataaatttg tttagttcaa ttcaatattc
     9361 aatgaaaaaa ttatttcttg tattgcgcta atgcttcttt taagtatcgt tgatgctcct
     9421 tcttaagtgt agccggtttc actactgtga ctgtattgcc atagctcaat attttttgca
     9481 ttaattcata attcgtcaaa ataaaatact cgaagtgaac ttctttatca tttgtcagca
     9541 cttcttcttg tgaccaatgc caaggtaatg ttctaatata tttagcctgc attggtgtga
     9601 agcttaaaat tacttgctca caagcagcac cttcatctat tattcctatc attctagaaa
     9661 aataagcgct aaggtcgatt tgtttatcct tcttaaattt gacttcagta atcgtcagcg
     9721 ataaaattct gtccacacca aattttacaa ttccttttct actcgttgaa caagcaacca
     9781 tataccaacg gccaagatat tcttttaaag catatgcttg caccgtataa tttttgggtt
     9841 tatccttttc aaaagcctga taactaaaat caatttgtaa ttgttgctga atagctgtta
     9901 atatttgcgg aataaattca acgcctttca acgcatgatt ttctttcaaa ataatggcat
     9961 tcgggtgttc tcctaatttt gcaaaatcaa gtaaattggc ttgtagcgtc atgtttttaa
    10021 taaactgaat gacattgtct gacatcgaat ctttttcaat aaaataacca cgattgaaac
    10081 tactatattc aataaaaatt ttaaattcag atttcagttc ttccaaatct cgctgtaagg
    10141 tgcgtgcagt tacagtatgt ccataatttt ctaaatactt tagtatgact tgtaacgacg
    10201 gatattttgt acgttctact ttgtttatta tcaaatcaaa gcgttttaat tttcctgtaa
    10261 gtgacattct ataaaaataa tgcttttgta tgacaaataa tgtcgtagta aaaacaaact
    10321 tatttcacaa tgcgcattat atttgcatga tgcgtgtagt tatccaacga gtagccaatg
    10381 ctagtgtaag tattgaaggc atagaaaaag gcaaaatcaa cacaggtttg ctcatatttt
    10441 taggtattga agaacaagac gatgcagcag atattgccta tttggtaaag aagattattc
    10501 cattgcgcat ctttagtgac gaagccgaaa aaatgaattt atcgatacaa gatattcatg
    10561 gcgatatttt actcatttca caatttacat tacatgcctc tacaaagaaa ggtaatagac
    10621 ctagttttat acgagccgct aagccagcaa gtgccattcc attatacgaa caatttatca
    10681 aggaaatgga gctacaatta ggcaaaaaaa tctgcactgg catttttggt gcagacatga
    10741 aaataacatt actcaacgat ggtccagtta ccataattat agacagtaaa aatccagaat
    10801 aaaagttgga aaattcctct cttaagataa tctaattttt gaaaaaacca agaaaaaaac
    10861 gaagtatttt ttattttact tcaaagtagc aaattgttat ttttttcatt ttcacaattt
    10921 ttttttgaaa aaacaattca cgtacgtgcc tataaaatgt taatacattt tatagtgttt
    10981 aagagtgcca ataactcatt ctatttcaag cgaattacca ttattaacat tcccaataaa
    11041 cagcttgtgg aaaagatata gaaaaattct aaatctatta aagtagcttt aagaagtcag
    11101 aaatgatgaa acacaaaaat cctgcctttc aattattatt tttttaaaaa aaactagtta
    11161 aaacgaacat agatgaatac agcattggct acggagaaaa ttttacaaga aagcgataat
    11221 cgtttcgtat tatttccgat taaacacgat gccatttggg cgatgtataa aaaacattta
    11281 gccgctcatt gggttacaga agaagtggac cttagccaag acattattga ctggaataat
    11341 aaattaaatg acaatgaacg tcattttatt aaacatgtat tagcattctt tgcagcaagc
    11401 gatggaattg taaacgaaaa cttggtggtc aacttcatgc aagatgtcac gattcctgaa
    11461 gttcgttgtt tctatggctt tcaagtagct atcgaaaaca tacatgcaga aatgtactct
    11521 ttattaatag atacatttat acgtgacact gcggagaaag acaaaatgtt taatgcgata
    11581 gaaacattag actgtgtaaa attaaaagca agctgggctt taaagtggat agaaacagca
    11641 ccttcattcg cacatcgctt agtagctttt gctgctgtag aaggcatttt cttcagcgga
    11701 agtttctgtt ctatttattg gcttaccgat agaggtttaa tgaaaggctt gggcaaatcc
    11761 aacgaattca tcagcagaga cgaaggtatg cattgcgatt tcgcttgctt gctctattct
    11821 atgctcgaaa acagattaag caaagaagag atatatcaaa ttatttgtga tgcagtagaa
    11881 tgcgaaaaag tatttgtcaa agactcattg ccagtgtctt taattggcat gaatgaaaaa
    11941 ttaatgtgcg aatatatcga gttttgcgcc gaccgtttga ttgtagcttt aggttaccct
    12001 aaagtatata acgcacaaaa tccttttcca tttatggaaa aaatatctac gcaaggaaaa
    12061 acaaacttct tcgaagaaag acctactgaa tatgtaaaag caggtgtaga atcaggcgag
    12121 aagaaatttg aaacagacga cgatttttaa taaacactta ctattgaaga tccaattgta
    12181 agttcaccta taaaagctat aaattatgta cgttatcaaa cgcgacggca gaaaagagcc
    12241 tgtccacttc gacaaaatta ctgcaagaat cagtaagtta tcttttggtt taaatgccac
    12301 ttttgttgtg cctcatcgcg tggcacaagc ggtaattcaa ggcctgtacg atggtgtaac
    12361 aaccacagaa ctcgatgagc ttgcagcacg caccagcgcc aatatgactg ttgttcatcc
    12421 agaatatgcc gtacttgcag cacgtattgc cgtatcgaac ttacacaaaa acacgaaaaa
    12481 gaatttctct cgcattatcg aagaattgta taattacatc gatccaaaaa ctggaaaaca
    12541 agctccgcta attgctgatg atgtctatga gataatcatg accaacaaag acaaattaga
    12601 tgcagccatc attcaccatc gcgactacaa ctatgatttc ttcggcttta aaactttaga
    12661 gaaatcttat ttattgaaaa tgcatggcaa agtagtagaa cgtcctcaac acatgttgat
    12721 gcgtgttgct gtaggtattc acaaaaatga catcgatgca gccatcgaaa cttatgattt
    12781 aatgtctgac aaatggttta cacacgcaac accaacacta tataatgcag gaacaccaaa
    12841 agcacaattg tcttcttgct ttttattaac catgaaagat gatagcatta aaggcattta
    12901 cgatacacta aaaaattgtg ccgagatttc acaagcagct ggtggcatcg gtttaagcat
    12961 acataatgta cgcgcaaaag gtagctacat caaaggaaca ggtggagaaa gcaatggtct
    13021 agtgccaatg ttacaagtgt ttaattcaac ggcacgttat gtcgatcaag gtggtggcaa
    13081 acgcaaaggc gcttttgcca tttatttaga accttggcat gctgatgtat ttgatttctt
    13141 ggaattgcgt cgtcctcatg gcaaagaaga aatgaaagca cgtgatttgt tcttcgcact
    13201 ttggatacca gatttattca tgaaacgcgt caaagaaaat ggcacttggt cattgatgtg
    13261 tcctaatgaa tgtcctggtt tggcagatac ttacggagat gaatttgaag cattatatga
    13321 aagatatgaa gccgaaggaa aattcagaaa acaaatcaaa gcacaagaat tatggttcca
    13381 tattttggat agccaaacag aaactggcac gccatacatg ttatacaaag accatgtcaa
    13441 cagaaaatct aatcaaaaga atttaggtgt cattcgttca agtaacttat gtacggaaat
    13501 tgtagaatat acttctgctg acgaaattgc ggtttgtaat ttagcatcta tcaaccttag
    13561 tcgcttcgta gaaaacaaca ccttcaattt ccaaaaacta tacgatatta cttacatcgt
    13621 tacgaaaaac ctaaacaaag ttatcgatgt aaatttctat cctgtacaag aagctcagaa
    13681 tagcaacatg cgtcatcgtc ctgtaggctt aggtgtgcaa ggcttagcgg acacgtttat
    13741 gatgttgcgc catccattcg acagcgaaga ggcacgcatc cttaataaaa atatttttga
    13801 aacaatatac ttcgctgctt tatcagcctc taaagatatc gcaaaagttg acggaccata
    13861 cgaaacattt aaaggttctc ctatttcaga aggtaaattc caattcgatc tatggaatgt
    13921 tacacctacc aaccgttggg actgggatac attaagaagc gaagtaatgc aacatggggt
    13981 tcgcaatagc ttgttagtag caccaatgcc aactgcatca acgtcacaaa tcttaggcaa
    14041 caatgaatgt tttgaaccct atacttctaa catctataca cgtcgtgtat tgagcggtca
    14101 atttatggtg gtaaacaaac atttactaaa agatcttatc gatttaggat tgtggagtga
    14161 agaaatgaaa aatgctatca ttgccaacaa tggttctatt caatctatcg aaggaattcc
    14221 tcaagaaaca aaagaattgt ataaaacagc atgggaaatt aagcaacgtg ccataataga
    14281 tatggctgct gaccgtggcg catttatcga ccaaagtcaa agcttgaatc tattcatgga
    14341 atcaccgaat tataaaaaat tgacatctgc tcatttctat gcttgggaaa aaggcttaaa
    14401 aaccggtatg tattacttac gttcccgtcc agcagtagat ccaattaagt ttactgttga
    14461 tgtagagaaa gcaaaaatga gtaatgctac tgcacaacaa caagatacaa acagcgataa
    14521 agtagcaagt atcgctccga tatatgaaag tgttgccaat gcacaacaag aaatgtttgt
    14581 tgtaacagag ccaaccattc aagaaaaatc tattgaagaa aaagccgcag aatttggtat
    14641 gagcgtagat gaattcttat cagcacaaaa agtgtgctca ttagaaaatc cgggcgagtg
    14701 cgaaatgtgc ggaagctaga aatacactaa ccaactggaa ttcaagcttt cccgtctcga
    14761 taatcgaggc gggatttttt ttgtgtatat ttgtgctcat ttttaaattt ttcgcttatg
    14821 tcatttttac aacagccttt tgcatggtat attgctgggc cactaattgc acttaactta
    14881 ttcctattat tatatttcgg acaaagattc ggaatatcta caggcttaaa aacaacatgt
    14941 gctgcactcg gcgctggttc catttctcaa ttttttaaat acaattggaa agaagaagct
    15001 tggttgttag tgtatattgt aggcacgata attggcggtt ttattgcttt tacctttatg
    15061 aatgcaggcg atgctgttat cgtgtctgac aacactaaag cagcactcga aaatatgaac
    15121 atcgtcgcca accaaacatt tgcgcctgat gaaatttttt caatagaaaa tttatttaca
    15181 ctaaaaggaa gtagcatttt ggtgattggt ggattgctga ttggttttgg cacacgctgg
    15241 gcaaacggct gcacatcagg acacgccatt tctggcttga gcgacttgca actgccatca
    15301 ttagttgctg ttatcggatt ttttatcggc ggattgctga tgacattttt attcattcct
    15361 ttaattttta aataaaatga gattattaaa atttttattg cttggtatat tttttggtat
    15421 tgtattagca aaagctcaag ttatttcatg gtttagaatc tatgaaatgt tcaggtttga
    15481 atcttttcac atgtatggta ttattggtag tgctgtcatc ttaggtgtgt tgattataca
    15541 acttataaaa agaaaaaaat tacgtgcatt taatggcgaa gcaattagca ttgcaccaaa
    15601 accaaacacg tataaagcaa gtatatttgg tggtatatta tttggcttag gttgggcctt
    15661 agtaggtgct tgtcctggtc ccatcttcat catgatagga gctggatttt ggagttttat
    15721 tgtagtattg gctggcgcat tagcaggtac ttttatttat ggattaataa aagaaaaatt
    15781 accacattaa gtaaataaat tctacttttg aaaatataaa ttcaattttt caacattaaa
    15841 aattatataa aatggcacac gaagacttag ctcctaaaga atttttagca gcctataatg
    15901 aacaaaaaga caatgctgta atattagatg tgcgaacagc agcagaaatt gcagatgcca
    15961 atattgatgg tcatattgca atagacatac aacaaccagg atttatggaa aaagtggcag
    16021 aattagataa aagcaaaacc tattttgtgt attgcagaag tggcaacaga agcggtcaag
    16081 catgcagata tatgtcaaca caaggatttg aaaagctata caacttgaaa ggtggcatga
    16141 tggcttggat ggaaactgat tttgaataat aacaacctac tttttattga caaaaggtta
    16201 ctgattgttt aagcaatagt tggtgaaatc ttaagttttc tattattatt gtatatacaa
    16261 cgatgctgac catattaggc atgataattg taaaattcat tagcgtaagc aacaatatta
    16321 ttgcttgcat taattaataa acaattatac aaatgaaaca catcattatt ttattcgcgc
    16381 tattgatagc ggcaacaacg acaccattgc aagcaaaaaa caaaaaatcg aaactcgcta
    16441 caatagacat cagcatgttt cctaaagcaa aaccaggata caaaatgctg tatattcaag
    16501 tgcctgcaac aaaacaagaa aacgatttaa aagtagaatt tttcgttgga agaaatgagc
    16561 aagtagattg caacaaatat ttcttaaccg gcgatattaa agaacaaaac ttagaaggct
    16621 ggggttatac ctattatacg gtagaatctg atggcaatat tgcaggtaca aaaatggctt
    16681 gtccagacaa tgtattacaa acaaagtttg ttcatttaca aggagaaatt acaagatata
    16741 acagcaaatt acctattgta ttgtatgttc cagagaaatt agacgtacaa tacagaatat
    16801 ggaaagcaga caaaaaaatg caacacgcaa aataacaatc actcaatatt aaatggagca
    16861 ataagtcaaa ttctacttga tttattgctc ttttttatgc ttatgaaaaa atatattctt
    16921 tgttcgatat tcattccgag tatgctgttc ggacagcaca agctcaaagt aaaaccaatg
    16981 gacagcttag cagtattaca atttgccaaa gaacgaaaat ggatatttca gcagcatgat
    17041 gacatgtcag aaactgcgcc tgtgtatgct ccaataataa gctatgacac gacgaaaaaa
    17101 caatggacga ttgtatcaaa gcgatacact acaacacgaa aaggtaaatg taaacatact
    17161 aatggttgca cgattgaaca cacgcaaatc ataattatag acgcaataaa taagcgacta
    17221 ataaaaagac aaatcaacaa aaaggcatat ccaaattacg aataagacaa tttccagcct
    17281 acaatttgat gaatttgcct aaataatgcc attactacga aaaatatcat tacgaaatct
    17341 tcatttttag ccttcataaa caatatcaaa atgctgaatc atcaataata ttgcttttta
    17401 attacatact atgtaattat tgcataattt tattgaaata tttgcataat tcaaattgct
    17461 tttttaattt tacaccatca agttctttac agcacaacaa aattgtgcct attgaaacca
    17521 cattgtgagg tgatgaaatt tggcagccat gccctcttgt ctcgggggtg aggagttcga
    17581 aataaagacg aaatagagca ctttagtgtg caggggttga ccaccaactt ttgttttgtt
    17641 cctaatcgcc ttgtgtaggt tcaagtccta ctctcacagc aataaaaaga ccgtctcgtt
    17701 ttaaatgaga cggtttttta tttacaacaa aaaaggaaca atcttactcg ttcctttcca
    17761 gcctaaacta ctttagccgc ttatcctttg aatagcatct actcgaggta ttgctctatt
    17821 ccaaacttgc gtttaagtag tgctgattac tcagtctatg tcgcacgcaa acacaaaatt
    17881 aataatctat ttcatattaa atgactaaag actttcaaaa aaagtcttta g
//
LOCUS       DAOQQV010000047        17863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_967_length_17863_cov_8.131514, whole genome shotgun sequence.
ACCESSION   DAOQQV010000047 DAOQQV010000000
VERSION     DAOQQV010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17863
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_967_length_17863_cov_8.131514"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /gene="serS"
                     /locus_tag="PLL59_06450"
     CDS             <1..1125
                     /gene="serS"
                     /locus_tag="PLL59_06450"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMY23482.1"
                     /translation="LSQRNTLSKEIGALMKEGKKEAAEQLKSNIAQIKDNTDLLEAQV
                     SDLENQIYERLVTLPNFPNSIVPAGKTPEDNEVVKSVGELPKLHVNAVPHWDLTKKYD
                     LIDFELGNKITGAGFPVYKGKGAALQRSLISFFLDKAIEAGYAEVQVPHVVNRDSGFG
                     TGQLPDKEGQMYHITQDDLYLIPTAEVPITNLYRDVIIDEKNLPIKNVGYTPCFRREA
                     GSYGKDVRGLNRLHQFDKVEIVRIEKPEHSYAALDDMLKHVESLLQALELPYRILRLC
                     GGDMTFSSALTYDFEVYSAAQQRWLEVSSVSNFETFQANRLKLRTKDADNKKSLAHTL
                     NGSALALPRIVASLLENHQTENGIKIPKALVPYTKFDMIS"
     gene            1233..1559
                     /locus_tag="PLL59_06455"
     CDS             1233..1559
                     /locus_tag="PLL59_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMY23483.1"
                     /translation="MIFISEKAKARVLKIWQDEHLTPEHFVRVSVTSGGCSGLTYNMN
                     FDNQSQPNDEIFEDKGIKLVVDPKSILYLLGTELDFSEGLNGKGFYFNNPNAARTCSC
                     GESFSL"
     gene            complement(1560..2585)
                     /gene="pheS"
                     /locus_tag="PLL59_06460"
     CDS             complement(1560..2585)
                     /gene="pheS"
                     /locus_tag="PLL59_06460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMY23484.1"
                     /translation="MDIFEKTTALLNEVNDATITSVESLETFRIRFLGTKNVLKDIFG
                     EIKNVPNERKKEFGQLVNSVKQAAEEKFAIHQAQLQSNTDVAENIDLSLPGDITTIGS
                     RHPIQLVKNRIIEIFSKIGFNVEEDREIEDDWHNFTALNMPEDHPARDMQDTFFINVN
                     PDIVLRTHTSSVQVRVMENQKPPIRILSPGRVYRNETISARSHCFFHQVEGLYIDENV
                     SFADLLQTLDFFVKELFGQDIKIKVRPSYFPFTEPSAEVDVTCFICGGGGCNVCKHSG
                     WVEILGCGMVDPKVLENCNIDPKKYAGFAFGMGVERITMLKYQIKDIRLMSENDTRFL
                     EQFKSAR"
     gene            complement(2651..3751)
                     /locus_tag="PLL59_06465"
     CDS             complement(2651..3751)
                     /locus_tag="PLL59_06465"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMY23485.1"
                     /translation="MKRLLLVSAITIFLFGCKNSSDNNVPEEPLSRIAFGSCCHQNLG
                     DKKIFDRILELNPQLYIAGGDNMYGDFFALAPGTKEYMEGAYRGLDASQSWKKLRASV
                     PIIATWDDHDTGENDATTTNPVKDIAKDLFFKYWNIPENDPRRSRPDGGIYGSYYYND
                     DEHRVQVLMLDLRYNHSPYKSSGPAAALSGYDTMLSPNATILGATQWTWLEEELRKPA
                     KVRIIMSSLQFNSLYDGSENWAVLPLEKQKMYDLIKSTGANGVFFLSGDVHYSELTKT
                     QPAGMYPIYDVTSSGITHHEDKVSPRNNSIRVGKGWQYVNFGFIDIDWKASPVSIKLQ
                     VFGNTNGSGPNGTLTRDDAKISHTVTLDELKF"
     gene            3906..4550
                     /locus_tag="PLL59_06470"
     CDS             3906..4550
                     /locus_tag="PLL59_06470"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HMY23486.1"
                     /translation="MFQDFKNDLRRALTLPLPGKEGQAPLKPYLQVNKSIDAPALPNK
                     RKGAVMSLIYPVNSIPHILFIERPIYDGVHSGQIAFPGGKIESSDPSNLAAALRETKE
                     EIGIESNIIDVVGPLSSVYVLASNFIVYPFVGIINEAPKIIAEAQEVSGVLEVPLYQF
                     LEPNVIKEKPIKSAFGLTLMAPYYDIQGKVLWGATAMMVSELCAVLQRQQIYLK"
     gene            complement(4553..5677)
                     /locus_tag="PLL59_06475"
     CDS             complement(4553..5677)
                     /locus_tag="PLL59_06475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HMY23487.1"
                     /translation="MHSDLIKIVSALLTSFVLTYMFIPSIIKVANIKHLFDTPGGRKS
                     HVQVTPTLGGIGIFGGFMISLCLFSQFYPGNGLRYILAALCFTFMLGAKDDIVELVAS
                     KKFIGQIFAASIVVILGDIRLTSLYGMFGISYIHDVFSIVLSIVTIIFIINAFNIIDG
                     INLLAGSISIVMSLAFGAWFYYYGFFDYAILAAAMSGAVFAFLKYNYTPAKIFMGDSG
                     SLSIGLMSAILAIQFIEKNEMILLSGKSEGFEIVAAPAMAIAVLIIPVYDTLRVFTLR
                     IINKKSPFMADRNHIHHRLIDLGLTHIQATFVLIGTNLFFIAIAYLLQSWGNLYLILT
                     EFILATVLTYVLFAIPLKRNNDTQKISHKKVNLKLETKAG"
     gene            complement(5729..6877)
                     /locus_tag="PLL59_06480"
     CDS             complement(5729..6877)
                     /locus_tag="PLL59_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMY23488.1"
                     /translation="MSKALKIGIVANTAFNIYNFRLSLILALKQEGHHVFAIAPYDDY
                     VPLLKENNIDYIEIKNLSRKGTNPITDLKLLNELRRLYKLHQLDAVLQYTIKPNIYGT
                     LAARLTKTIAICTVTGLGYTFLNKSTASKIARRLYRFAFSFTHKVLFQNEDDLAVFVQ
                     NNLVDKAKTQIVLGSGIDVEKFQPQFCLNMPSSQLPFAKTKHQISFLLIARLLKDKGV
                     YEFVEAAKQMLQTSTLATFHLLGDIDTNNPSAIDKATISQWENEGIIKYYGFTKDTRP
                     YICAADCIVLPSYREGMPRVILESMAMGKPSITTDAPGCKDAIVHQVSGWICKAQDSN
                     SLRDAMQMFLELDEVAKLKIGLNARNRAEEIFSTTAVNLFYINLLKNL"
     gene            complement(6927..8822)
                     /gene="asnB"
                     /locus_tag="PLL59_06485"
     CDS             complement(6927..8822)
                     /gene="asnB"
                     /locus_tag="PLL59_06485"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HMY23489.1"
                     /translation="MCGIAGAYSFKNKLVEQQCLQNAATSLNKRGPDNQNFVTIGKAS
                     LVHARLSIIDTSVAANQPIYDITQRYAIIFNGEIFNYQSVRDELLQKGVQFKTHSDTE
                     VLLYAYIHFGESFLHKLNGFFAFAIHDKQEDTLFIACDRFGIKPLYYYSDDDFFFFGS
                     ELKNILQFPIKKEIDWSAVSLYFQMNYIPGKNAIFKNIQRLEPGQQLSIRNNNIEIKR
                     WYNIPYNALNAEQNQLSYEEQKKKLLALMDDSVRLRLIADVPLGAFLSGGIDSSVVTA
                     MASRHTPHLNTFSIGFESNAFFDETPYANLVAKHFKTNHTVFKLKNDDLFESLHELLD
                     YSDEPFADSSALLVNILSKFTRQHVTVALSGDAGDELFAGYNKHYGEWQMRNNSWQAS
                     IIHLLQPVWEKLPKSRSGNIANKIRQLERFAKGMDLKPAERWWQWCCINDTNYVEQLI
                     GDKADLSITEMIKIRQQYASLVQYDGTLNDCLLADVNMVLPYDMLTKVDRMSMRNSLE
                     VRVPFLDYRIVEFAFSLPVSSKINGNMKKKIVQDAFREVLPNELYNRPKKGFEVPLLQ
                     WFRTDLKDKILNDWLSDEYIQQQGVFNINTIQQLKLQLFSNNPGDVHAQIWALIVFQN
                     FWRKYMD"
     gene            complement(8892..9770)
                     /locus_tag="PLL59_06490"
     CDS             complement(8892..9770)
                     /locus_tag="PLL59_06490"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HMY23490.1"
                     /translation="MTIGIYSRVFKEEHTPFVLELILQLTQKQIPFLIYDTYYKSIKK
                     YIAKQKIQTFKNHNDVKNNIDLLLTLGGDGTLLDTLQLVRDSEIPVAGINMGRLGFLA
                     DIHKSEIKALVESIQQNTFTIEDRTLICIETTKPVFGDVNYGLNEFTIHKTDSSSMIV
                     VHVFLNGEFLNSFWADGLIVATPTGSTAYSLSCGGPIVFPSSNNFVLTPVAPHNLNAR
                     PIIIADNTVLSFEVECRGNNFLCSIDSRSVNIDNTIQLAVRKANFSFKLLRMPESSYL
                     STLREKLNWGEDSRNS"
     gene            complement(9821..11221)
                     /locus_tag="PLL59_06495"
     CDS             complement(9821..11221)
                     /locus_tag="PLL59_06495"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HMY23491.1"
                     /translation="MLIIMLGAHAQDSIHYLKVRHINIAGNKKTKDFVIHREMTIHEN
                     DSFPAKEMDALLLQQRLNIFNIGLFNDVSINIKNWEDDSLDLSIQVHERWVIIPLPII
                     KFVDRNVVEWWKNYRHDFKRLQYGAQVNWRNLTGRNDALQLGISFGFAQRLDIGYAVP
                     QFNIRKEKVGMSMYFTMLRTKRIAYATTNDALSFMNLGTSWQQQKIEVATQVTYRNAI
                     HNTHYFTIGYGLTVISDSVRNANPNYFLNNDRKQHYFKVGYTIIADHRNLRSYPTAGW
                     YLQASLTNYGLGFMRTRMTTTSFQLSKYFQWKKHQRFSGAAMIKWQFSWPFKQPYNLQ
                     NTKSLGYDENTIRGYELNVIDGQHYLLFKNEYRFRVFSFQMSKIKKFKGKNAAILNSS
                     LAYLPLNLYLTAYFDAGYVWDNYFTANNQYKNKWQFGYGIGLNFVTFNQKLFRIEYSI
                     NRYLKKGVYLHFEQPL"
     gene            complement(11337..11945)
                     /locus_tag="PLL59_06500"
     CDS             complement(11337..11945)
                     /locus_tag="PLL59_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23492.1"
                     /translation="MIKIISKIVSYIFHPLLMATWFVLLLLYTNPFSFAGMPNGIVIG
                     VVFVNTFMFPAIALLLMRKLDFIDSLELPDKKQRIIPLVATIVFYVWAFLAIKKLNFP
                     FMVGIFMLGSVVSLFLAFFINIFSKISLHMVGISGALTAIMLLVFISPADVSYYFLLL
                     IVLTGAVATARLYLNAHTIKEVYAGFIVGMIGQIFGLILYHP"
     gene            complement(11992..13470)
                     /gene="rpoN"
                     /locus_tag="PLL59_06505"
     CDS             complement(11992..13470)
                     /gene="rpoN"
                     /locus_tag="PLL59_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HMY23493.1"
                     /translation="MLHQRLSQKMLQKLSPQQIQLMKLLQVPTANLEQRIKEELEINP
                     ALEESISDFDNDKEDLSEPEQIRDDEENYENNNEDDDFADSLDIQEYTKDDYDYTNDD
                     GDYRGKEDKKQTPLAIGKTFHDFLDEQLHLLDLDEHEWQIADQLIGSIDDDGYLRREL
                     DAIVDDLAFQKNVSTSVEEIEEVLRTIHRFDPPGVGARNLQECLQIQLERKEQNSHVK
                     LALRIITLQFEAFTKKHYDKLLKSLDIEEEQLKKALDVILHLNPKPGGASDNTTAVNH
                     QTIIPDFTVENNNGELEVLLNGKNAPDLRISDSFKEMLSSYGKGAMKDKKQKEAVVFI
                     KQKIDAAKWFIDAIKQRQHTLFVTMEAIVDFQKEYFLSGDEMKLRPMILKDIAEITSL
                     DISTVSRVANSKYCQTEFGVFSLKHFFSEGLQTEGGEEVSTREVKKILEDLIAVEDKS
                     KPLSDEFLTEQLKEKGYNIARRTVAKYREQLNIPVARLRKEL"
     gene            complement(13517..14941)
                     /gene="asnS"
                     /locus_tag="PLL59_06510"
     CDS             complement(13517..14941)
                     /gene="asnS"
                     /locus_tag="PLL59_06510"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HMY23494.1"
                     /translation="MRKIYIKEILENTSVGSDVVVRGWVRTKRNSKNVTFINVSDGTT
                     IHTIQAVVEAETISDDLLAKINTGACLEIQGQLIASQGTQKVEVAVKNIIILGEADAE
                     KYPIQPKKHSFEFLREKAHLRPRTNTFGAILRIRHALAFAIHNYFNDQGFFYLHTPII
                     TGSDAEGAGEMFRVTTLDATNPPKTEEGKIDYSQDFFGKETNLTVSGQLEGELGAMAL
                     SKIYTFGPTFRAENSNTSRHLAEFWMIEPEVAFNDLVDNMDLAEDFLKYLINYALSHC
                     KEDLEFLDKRFAEEEKQKPTQERSELGLLDKLKFCVENEFQRMTYTEAIEILKTAKKK
                     FQYPVDWGTDLQSEHERYLVEKHYKKPVILTDYPEHIKAFYMKLSEDGKTVRAMDVLF
                     PGIGEIIGGSQREENYDKLKKRAHQFGIEEESIWWYLETRQFGTCPHAGFGLGFERLV
                     LFVTGMGNIRDVIPFPRYPKSAEF"
     gene            15074..16024
                     /locus_tag="PLL59_06515"
     CDS             15074..16024
                     /locus_tag="PLL59_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HMY23495.1"
                     /translation="MSFIPVLDLADYLSGDETRRQRFIQGFGKAYSEIGFAAIDNHGI
                     PQAVIDKYYHLVEQFYALPLESKRGYEKVEYAGQRGYTSFGREKAKQSEVADLKEFWQ
                     VGQYIEDGELMPAADYPDNIEVAELPEFNETGRQLYKSFENSGRHLLRAIAEYLDLDV
                     HYFDAKIHNGNSILRAIHYPPITQEPKSAIRAEAHEDINLITLLVGASADGLQVMDVD
                     GNWHDAKAEPHQIMVNVGDMLQRLCNNKLKSTTHRVVNPPKEKWHTHRYSIPFFLHPR
                     SEVRLDCLENCIDATHPIAYEPITAGEYLDERLREIGLKK"
     gene            16064..16810
                     /locus_tag="PLL59_06520"
     CDS             16064..16810
                     /locus_tag="PLL59_06520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMY23496.1"
                     /translation="MPSALSIKIGDFLYKNAFPIYNVIYPFFKRKQDKHEIALLKQYV
                     KKGDIVLDIGANIGFYTKILAELVGENGKVYAFEPDKTNFSYLMKNAGHLRNVEFHNK
                     AVSEKSGKITLYHSDLLNVDHKTYATENYTSTSEIECVAIDDIIPNHKVDFIKIDIQG
                     YEYFAFQGMQTVFQKNETLKIITELYPLGMQNAKIDAIAFFNLLWENNFIVYKSEENS
                     SSEFTINDIKQYTSIKDEYTFVDLFIEKRN"
     gene            16812..>17863
                     /locus_tag="PLL59_06525"
     CDS             16812..>17863
                     /locus_tag="PLL59_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23497.1"
                     /translation="MAKKQPIRNQQQPVATNKKNTTRTTNAISKSMFDILETHFSAKR
                     NMYLILLVLISFIFALFCFDNKISTANDDALYIESGARYANAFFAKTSWYTANAPLYP
                     IVLGLIIKVIGVRLMWLKMFSVISFAAAIYFIFRTYEKRIPYLILIPSLLLTAINFEY
                     LMYASLTYTETFSLMIIGICFFLIFKIFDKLQIEGLSIQDKILPVFILGALCLLLMLT
                     RSVALGAIGVVVLYLVYRKNYKEAVLAVASFVIVFLAYKFLVDAIWQPSDSQFKTQIK
                     LMYQKDVYNPQLGDEDLSGFIVRFWKNCEIYISSRFMFVLGFREEMAETNTLVTIICI
                     AIVLWSLYLAHISKQYT"
BASE COUNT         5689 a         3217 c         3009 g         5948 t
ORIGIN      
        1 ttgtcacaac gcaatacatt gtcgaaagaa attggtgcgc tgatgaaaga aggcaaaaaa
       61 gaagcagcag aacaacttaa atcgaatatt gcacaaatca aagacaatac cgacttgctg
      121 gaagcacaag ttagcgactt agaaaatcaa atttatgagc gactggttac gcttccaaat
      181 tttccgaata gtatagtgcc tgctgggaaa actccagaag ataatgaagt cgtaaaatct
      241 gttggcgaat taccaaaatt acatgtcaat gcagtaccgc attgggattt gacaaaaaaa
      301 tatgatttaa tagattttga attaggcaat aaaatcacag gcgctggttt tccagtgtat
      361 aaaggaaaag gcgcagcact gcaacgttca cttatttctt ttttcttaga caaagccata
      421 gaagctggtt atgcagaagt gcaagtgcct catgttgtta atcgagattc tggttttggc
      481 actggacaat tgcctgacaa agaaggccaa atgtatcaca tcacacaaga cgatttatac
      541 cttattccaa cagcagaagt acctattact aatttatatc gtgatgtcat catcgatgag
      601 aaaaatttac ctataaaaaa tgttggctac acaccctgtt tccgtcgcga ggctggaagc
      661 tacggtaaag atgtgcgtgg tttaaatcgt ttgcatcaat tcgataaagt agaaatcgtg
      721 cgcatcgaaa aacctgaaca ttcgtatgcc gcattagacg acatgctaaa gcatgtagaa
      781 agtttattgc aagcattaga attgccctat cgaatcttgc gtttatgtgg tggcgacatg
      841 acattttcat cagcattaac ttatgatttt gaagtgtatt ctgcggcaca acaaagatgg
      901 ttggaagtaa gttctgtttc aaattttgaa actttccaag ccaatcgatt aaagctaaga
      961 acaaaagatg cagacaacaa aaaatcattg gcacacacac ttaatggaag tgcattagca
     1021 ttgccacgca tcgtagcaag tttattagaa aatcatcaaa cagaaaatgg tattaaaata
     1081 ccaaaagcat tggtacctta tacgaagttt gatatgattt cgtaatttat atgctttgtt
     1141 ttatttgtta gactatttaa acttttaaca cacaaaatgt gtttagataa atgaattttc
     1201 tagattaata atttctaatt ctaattttat acatgatatt tatttctgaa aaagcaaaag
     1261 cacgtgtctt aaaaatttgg caagatgaac acttgacacc agagcatttt gtgcgtgtat
     1321 ctgtcaccag tggcggttgt agtggcttga catataatat gaattttgac aatcagtcac
     1381 agcctaatga tgaaatattt gaagacaaag gcatcaagct tgttgtagat ccaaaaagta
     1441 ttttatacct acttggcaca gaattagatt tttcggaagg gctgaatggc aaaggctttt
     1501 atttcaacaa tccgaatgcc gctcgcacat gtagttgtgg tgaaagtttt agtttgtaac
     1561 tatctcgctg atttaaactg ctctaaaaat cgcgtatcat tttcagacat tagacgtatg
     1621 tctttgattt gatatttcaa cattgttata cgttcaacgc ccataccaaa ggcaaatccg
     1681 gcatactttt tcgggtcgat attgcaattt tccaacactt ttggatctac cataccacaa
     1741 cctaatattt cgacccaacc gctatgtttg cagacattac agcctccgcc accgcatata
     1801 aaacaagtaa catctacttc tgcactcggc tctgtgaatg gaaaataaga aggtcttact
     1861 tttattttta tatcttgtcc aaaaagttct tttacaaaga aatctaaggt ttgtagtaaa
     1921 tctgcgaacg aaacattttc atcgatatat aaaccttcta cttgatggaa aaaacaatgt
     1981 gagcgtgctg aaatagtttc atttctatat acacgtccag gtgataaaat tcggataggt
     2041 ggtttttggt tttccataac acgcacttgc acagaagaag tatgcgtgcg caatacgata
     2101 tctggattaa cattaataaa aaaagtatcc tgcatatcgc gcgcaggatg atcttccggc
     2161 atatttaatg cagtaaaatt atgccaatca tcttcaattt ctcggtcttc ttctacatta
     2221 aaaccaatct tagaaaatat ttcgataatt ctatttttga ccaattgaat gggatgcctt
     2281 gagccaattg ttgtaatatc tccaggcaaa cttaggtcga tattttctgc cacgtctgta
     2341 ttgctttgta attgtgcttg atgtatggca aatttctctt cagctgcttg ttttacgcta
     2401 ttgactaatt ggccaaattc tttttttcgt tcattcggca cgttttttat ttcgccaaag
     2461 atatctttta agacattttt agttcctaaa aatcgaatgc ggaaagtctc caatgattcg
     2521 acagaagtaa ttgtcgcatc atttacttca tttagtagtg cagttgtctt ttcaaaaata
     2581 tccatacttc aaaaataaaa aaaagaggca catgatatgc ctctttgtct atattgtaat
     2641 gattaattaa ttaaaatttg agttcatcta aagtaacagt gtgcgaaatt ttagcatcgt
     2701 cacgtgttaa agtaccattt ggaccagaac catttgtgtt gccaaacact tgtaatttta
     2761 ttgacaccgg agatgctttc caatcgatat cgataaaacc aaagtttaca tattgccaac
     2821 cttttccaac tctgatgctg ttatttcttg gagacacttt gtcttcgtga tgcgtgatac
     2881 cgctcgacgt aacgtcataa attggataca taccagctgg ttgcgttttt gtcaattcag
     2941 aataatgtac gtcaccactt aagaaaaaca cgccgtttgc tcctgtgctt ttgattaaat
     3001 catacatttt ttgtttttct aatggtaaaa ctgcccaatt ctcactacca tcatacaaag
     3061 agttaaattg caacgaagac ataatgattc ttacttttgc tggttttctt aattcttctt
     3121 ctaaccatgt ccattgtgtt gcgcctaaaa tggtagcatt tggagacaac atcgtatcat
     3181 aaccactaag agctgctgct gggccacttg atttgtatgg cgagtgatta taacgcaaat
     3241 ccaacattag cacttgtact ctgtgttcgt cgtcattata ataataagat ccataaattc
     3301 caccatctgg gcgtgaacga cgtggatcat tttctggtat attccaatat ttaaaaaata
     3361 aatcttttgc aatgtcttta actgggttgg tagttgttgc gtcattttcg ccagtatcat
     3421 ggtcgtccca agtagcaata ataggtacgc tagctctcaa ttttttccat gattggctag
     3481 cgtctaatcc acgataagca ccttccatat attcttttgt gccaggtgcc aacgcgaaga
     3541 aatcgccata catattatct ccaccagcaa tatataattg tgggtttaat tccaatattc
     3601 tatcaaatat ttttttgtca cctaaatttt gatgacaaca tgaaccaaat gcaattctag
     3661 acaaaggttc ttccggaaca ttgttatcag aactattctt acagccaaac aagaatattg
     3721 ttatagctga tactaacaaa agacgtttca tttagatttt tttaagtgag ttgcgaatgt
     3781 ataaatttat gcatttttag tgaattttaa cgcttaccta taaacgattt aacatatttt
     3841 tttcaaatta actacacttt tagcattgta tttgaatttc gacatagaac atatattttt
     3901 gaatgatgtt tcaagatttt aaaaacgatt tacgacgtgc acttacattg ccattgcctg
     3961 gtaaagaagg acaagcgcca ttaaaaccct atttgcaagt taataaatca atagacgcac
     4021 cagcactacc gaacaagcga aaaggtgccg ttatgtcttt aatctatcct gtcaatagta
     4081 ttcctcatat cttgtttata gagcgaccta tttacgacgg tgttcacagt ggacagattg
     4141 catttcctgg tggtaaaatt gagtcgagtg acccttcaaa tttagctgct gcattgcgcg
     4201 aaaccaagga agaaatcggc atagagtcaa atattataga cgttgttggt ccgctttcga
     4261 gtgtatatgt gttagccagc aattttatcg tatatccatt tgttggcatc atcaatgaag
     4321 cacctaaaat aattgctgaa gcgcaagaag tatcaggtgt tttagaagta cctttatatc
     4381 aatttttaga gccaaatgtc ataaaagaaa aaccaataaa aagtgcattt ggattaacct
     4441 taatggcgcc ttattatgat attcaaggga aagtattgtg gggcgcaacg gcgatgatgg
     4501 ttagcgaact ttgtgctgta ttacaacgac aacaaattta tttgaaataa gcttatcctg
     4561 cttttgtttc caacttcaaa ttgacttttt tatggctaat tttctgtgtg tcattatttc
     4621 tttttaatgg aatagcaaac aggacatagg ttagcacggt tgccaatata aactcagtta
     4681 gaatgagata taaatttccc caactttgta ataagtaagc tatagcaata aaaaacaaat
     4741 tagtgcctat aagtacaaat gttgcttgta tatgcgtcaa gcctaaatct attagacgat
     4801 ggtgaatatg atttctatct gccataaatg gtgatttttt gttaatgata cgtagcgtaa
     4861 acacacgcaa tgtatcatat acaggaatta tcagcacggc aattgccata gccggtgcag
     4921 ccacgatttc aaaaccttcg ctcttgccgc ttaataatat catctcattt ttttctatga
     4981 actgaatcgc caatatagcc gacattaagc cgatgctcag gctacctgag tcgcccataa
     5041 atatttttgc tggcgtataa ttatatttta aaaatgcaaa cacagcacca ctcattgccg
     5101 ccgctaatat ggcataatca aaaaagccat agtaataaaa ccaagcgcca aatgctaaag
     5161 acattacaat agaaatactt cctgcaagca aatttattcc atcaataata ttgaaagcat
     5221 tgataataaa aatgatggtg acaatagata gaacaatgct aaatacatcg tgaatatatg
     5281 aaattccaaa catgccataa aggctggtca gtctgatatc acctaatatt actacgatac
     5341 ttgctgcaaa tatttgaccg ataaattttt tagaagcgac caattctacg atatcatctt
     5401 ttgcacccaa cataaatgta aaacacagag ccgctaaaat atatcgaaga ccattgcctg
     5461 gataaaattg agaaaacaag caaaggctta tcataaaacc gccaaaaatt ccaattcctc
     5521 ctaatgttgg agtgacttgc acatgtgatt ttctaccgcc aggcgtatca aaaagatgct
     5581 taatgttggc cacctttata atggaaggta taaacatata cgtaagtacg aaagaagtca
     5641 gaagtgcaga aacaatttta attaagtcgc tatgcatatg ttgtgttaac aaatttaatg
     5701 ctttttattt aaataacgtt aaaattgact atagattttt gagtaaattg atataaaaca
     5761 aattgactgc cgttgtagaa aatatttctt ccgctctatt tctggcattt aagccaatct
     5821 ttaattttgc cacttcgtca agttcaagaa acatttgcat ggcatcacgt aaggaatttg
     5881 agtcttgtgc tttacaaatc cagccactca cttggtgtac gatggcatct ttacatcctg
     5941 gcgcatctgt cgtgatactt ggttttccca tggccatact ttctaaaatc acgcgaggca
     6001 tgccttcgcg atacgatggc aaaacaatac aatctgcagc acatatatat ggtcgcgtgt
     6061 cttttgtaaa accataatat tttatgatgc cctcattttc ccattgcgaa atcgttgctt
     6121 tatcgattgc tgatggatta ttggtatcaa tatcgccaag cagatgaaat gttgcaagag
     6181 ttgaggtctg aagcatttgt tttgctgctt caacaaattc atacacacct ttatctttca
     6241 gtaaacgagc tatcaataaa aagctaatct gatgcttagt tttcgcaaaa ggcaattgcg
     6301 aagatggcat attcagacaa aactgtggct gaaatttttc gacatcgatg cccgaaccta
     6361 gaactatttg tgtttttgct ttatctacta aattattttg cacaaaaaca gcaaggtcat
     6421 cttcattttg aaataagact ttatgcgtaa aggaaaatgc gaatcgatat aaacgacgtg
     6481 ctattttact tgcagtgctt ttatttaaaa aagtatagcc taaaccagtt accgtacaga
     6541 ttgcgatggt ctttgttaat cgcgcggcca atgttccata aatattaggc ttgatggtgt
     6601 attgcaaaac agcgtctaat tgatgcaact tatacagacg ccgcaactca ttcagtaatt
     6661 ttaaatcagt gattgggttc gtgcctttgc gtgacaggtt ttttatttcg atataatcga
     6721 tgttattttc tttcaacaat ggaacatagt catcataagg cgcgattgca aacacatggt
     6781 gtccttcttg ttttaatgca agaattaaac ttaatctgaa attataaata ttgaatgctg
     6841 tattggctac gatgcctatt ttgagtgcct tgctcatgtt tttcaaagat aattttttca
     6901 ctcaaaaaat aaaatgtgtt tttgcatcaa tccatatatt ttcgccaaaa attttgaaac
     6961 actatcaatg cccaaatctg tgcgtgcaca tcgcctggat tatttgaaaa taattgcaat
     7021 tttaattgtt gtatcgtgtt tatattaaaa acgccttgtt gttgtatata ttcatcactt
     7081 aaccaatcat tcagtatctt atcttttaaa tctgttcgga accattgtag caaaggtact
     7141 tcgaagcctt ttttaggtcg attgtacaat tcatttggca atacttctct aaatgcatct
     7201 tgcacaattt tctttttcat attgccattt attttactgg aaacgggcaa cgaaaacgca
     7261 aattctacaa ttctataatc tagaaacgga acacgcactt ctaaggaatt gcgcatactc
     7321 attctatcta ctttggtgag catatcatac ggcaatacca tgttcacatc agccaataaa
     7381 caatcgttaa gcgtgccatc atactgcacc aatgatgcat actgttgtcg aatttttatc
     7441 atttccgtta tagacaaatc tgctttgtca ccaatcaatt gttctacata gttagtgtca
     7501 tttatacaac accattgcca ccatcgctct gcaggtttta aatccatgcc ttttgcaaaa
     7561 cgttctagtt gtctaatttt attagcaata tttccgcttc tcgatttagg caatttttcc
     7621 caaactggct gcaacagatg tattatgctg gcttgccaac tattatttcg catttgccat
     7681 tcgccatagt gcttattata accagcaaat aactcgtcgc cagcatcacc agatagtgca
     7741 acagtgacgt gttggcgtgt aaattttgat aaaatattga ccaataatgc tgaggaatct
     7801 gcaaaaggtt cgtctgaata atctaacaac tcgtgtaaac tttcgaacaa atcatcattc
     7861 tttagcttaa acacggtatg gttcgtttta aaatgttttg ccaccaaatt agcgtatggt
     7921 gtttcatcaa aaaaagcatt gctttcgaaa ccaatagaaa atgtattgag atgaggcgtg
     7981 tgtcgcgacg ccattgctgt tacaacagag gagtcaatac caccacttaa gaatgcacca
     8041 agaggcacat cagcaattag gcgcagcctt acggaatcat ccatcagcgc gagcaatttc
     8101 tttttttgct cttcgtaact aagttgattt tgttccgcat ttaatgcgtt gtatggaata
     8161 ttataccatc tctttatctc tatattgttg ttgcgaatcg agagctgttg acctggttct
     8221 aagcgttgaa tatttttgaa aatggcattt tttcctggaa tgtaattcat ttgaaaatac
     8281 aaactcaccg cactccaatc tatttctttt ttgataggaa attgcaaaat atttttcaac
     8341 tctgagccaa agaaaaaaaa gtcatcatcg ctataataat acaatggttt gatgccaaat
     8401 ctatcacaag cgataaacag tgtgtcttct tgtttatcgt gaatcgcaaa agcaaaaaat
     8461 ccatttagtt tatgcaaaaa agattcgcca aaatgtatgt atgcatacag caaaacttct
     8521 gtatcagaat gtgttttaaa ctgcacacct ttttgcagta gctcatcgcg aacagactga
     8581 tagttgaaaa tttcgccatt gaaaataatc gcatatcgtt gtgtgatgtc ataaatgggc
     8641 tggttggccg ctacagatgt gtcaattata gaaaggcgag catgcactaa agaagctttg
     8701 ccaatggtca caaaattttg attgtctggc cctcttttat ttaaagaagt cgctgcattt
     8761 tgcaaacatt gttgttctac taatttattt ttaaatgaat atgcacctgc aattccacac
     8821 attttacatt gttatttatg ctgtacaatg tacgattttt atttttactt gaaaatgaga
     8881 atcatcgatt cttagctatt tctgctgtct tcgccccaat ttagtttttc gcgtaaagtg
     8941 cttaagtaag agctttctgg catacgcaat aatttaaacg agaaattggc tttgcgcact
     9001 gctaattgta ttgtgttatc aatatttact gagcgtgaat caatagaaca tagaaaatta
     9061 ttgccacggc attctacttc gaaagaaagc actgtattat cagcaataat aataggtcga
     9121 gcattgagat tgtgtggtgc caccggtgtg agcacaaaat tatttgaact tggaaaaaca
     9181 attggaccgc cacaactcaa agaatatgca gtagagcctg taggcgtcgc tacaattagg
     9241 ccatctgccc agaatgaatt taaaaattcg ccattcaaaa acacatgcac tacaatcata
     9301 gaagaagagt cggttttatg aatagtaaat tcatttaatc cataattcac atcgccaaaa
     9361 acaggctttg ttgtttcgat gcaaatcagt gttctgtctt caattgtaaa agtattttgc
     9421 tgtatagact ctactaatgc tttaatttca gatttatgaa tatccgctaa aaaaccaagc
     9481 ctgcccatat taatgccagc aacaggaatt tcgctatcgc gcacgagttg taaggtatct
     9541 agcaaggttc catcgccacc taatgtaagc aataaatcga tgttgttttt tacatcattg
     9601 tgattcttaa atgtttggat tttttgcttt gctatgtatt tctttatgga tttataatag
     9661 gtatcataaa ttaagaatgg aatttgtttt tgtgtcaatt gcaaaatcaa ctctaacaca
     9721 aatggtgtgt gttcttcttt aaatacgcga ctataaattc ctattgtcat ttaatcaatt
     9781 attaaattgc aagctcattg ctgtttattg acaaacaaag ctacagtggt tgctcaaaat
     9841 gtaaatatac tcctttcttc aaatatctgt taatgctata ttcgatacgg aaaagctttt
     9901 gattgaaagt gacaaaattt aatccgatgc cataaccgaa ctgccatttg tttttgtatt
     9961 gattattcgc agtaaaataa ttgtcccaaa catagcctgc atcgaaataa gccgttaaat
    10021 ataaattgag tggcaagtaa gccaatgaag aatttaaaat ggcagcattt tttcctttaa
    10081 attttttaat cttgctcatt tgaaagctaa acactctaaa gcgatattca tttttaaaca
    10141 acaaataatg ttgtccatca attacattta gttcataccc acgaattgta ttttcatcat
    10201 agccaagtga tttggtattt tgtaaattat acggttgttt aaaaggccac gaaaactgcc
    10261 attttatcat ggctgctcct gaaaagcgtt gatgtttttt ccattgaaaa tatttactca
    10321 attggaagct tgtcgtcgtc atgcgtgttc tcataaatcc taagccatag ttcgttaaac
    10381 tggcttgtaa ataccagcct gccgttggat aagagcgtag atttctatgg tcagcaatta
    10441 tggtatatcc tactttaaag tagtgttgtt ttctatcatt atttaaaaaa taatttggat
    10501 tagcattgcg tacagaatca ctgatgacgg taagaccata acctattgta aaataatgtg
    10561 tattatgaat cgcgtttcta taggtaactt gtgtagccac ttctattttt tgttgctgcc
    10621 aagatgttcc caaattcata aatgacaatg catcgtttgt tgttgcatag gcaattcgtt
    10681 tggtgcgcag catggtaaag tacatcgaca tgcctacttt ctctttccgt atattgaatt
    10741 gtggaacggc gtatccgatg tctaatcttt gagcaaagcc aaagctaatt cccaattgca
    10801 gtgcgtcatt tctgccagtc aagtttcgcc aattcacttg tgcgccatac tgtaatcgct
    10861 taaaatcgtg tctataattt ttccaccatt caacaacgtt acggtcaaca aacttgatga
    10921 tgggcaatgg aataatgacc catctttcat gtacttgtat tgataaatcc aagctatcat
    10981 cttcccaatt ttttatgttt atggatacat cattaaacaa accaatatta aaaatattta
    11041 aacgctgctg caataataaa gcatccattt ctttggctgg aaaagagtca ttttcgtgaa
    11101 tcgtcatctc acgatgaatg acaaaatctt ttgtcttttt attgcctgct atattgatat
    11161 gtctgacttt taaataatga atgctatcct gtgcgtgtgc accaagcatt attattagca
    11221 taaaaagaaa aactattttt ttcaaataca acgatgatat taaacaaagt tacgagaata
    11281 aatgtgctat tagcgatttt agtttttgtg caattgtgaa aaagtgtttt ttcgatttac
    11341 ggatgatata gtattaaacc aaatatttga ccaatcatac cgacaataaa accagcatat
    11401 acttctttta ttgtgtgtgc atttaaatac agccttgctg tcgctactgc tcctgttagc
    11461 acgatgagca aaagaaaata atagctaaca tcggcaggtg agataaacac caataacatt
    11521 attgcagtaa gcgcgccact gatgccgacc atgtgtaagc ttatttttga aaaaatattt
    11581 ataaaaaatg caagaaatag agacacaaca gagccgagca taaaaatacc taccataaat
    11641 ggaaagttta attttttgat ggccaaaaat gcccaaacat aaaatacgat tgttgctact
    11701 aagggaataa ttctttgttt cttatcaggc aattccaagc tatcgataaa gtctaattta
    11761 cgcatgagca atagtgcaat tgcaggaaac ataaatgtat tgacaaatac aacgccaatc
    11821 acaattccgt taggcatgcc agcaaatgaa aatggattgg tgtacaacaa taataacaca
    11881 aaccacgttg ccattaataa tggatgaaat atataggaaa caatttttga gattattttt
    11941 atcatgttaa tacaatcagt gttttattgt tcaaatttac aataagtggc tttacagttc
    12001 tttgcgcaat cgcgcaacag gaatattgag ctgttctcta tattttgcta ctgttctgcg
    12061 agcaatatta taaccttttt ctttcagctg ttcagtcaga aactcatcgc ttaatggttt
    12121 tgatttgtct tccactgcaa ttaaatcttc caatattttc ttcacttctc gtgttgatac
    12181 ctcttctcct ccttctgttt gtaagccttc ggaaaaaaaa tgtttcaaag aaaaaacacc
    12241 gaattctgtt tggcaatatt tgctatttgc cacgcgtgat acagtagaaa tatccaagct
    12301 tgtaatttct gcaatatctt ttaaaatcat tggacgtagc ttcatctcat cgcctgataa
    12361 gaaatattct ttttgaaaat cgacgattgc ttccatcgtt acaaataaag tatgttggcg
    12421 ttgttttatg gcatcgataa accactttgc tgcgtctatt ttttgtttga taaacacaac
    12481 agcttccttc tgttttttgt ctttcatagc gcctttgcca tacgatgaaa gcatctcttt
    12541 aaacgagtcg ctgattctta aatcaggtgc atttttacca tttaacaata cttctaactc
    12601 accattatta ttttcgactg taaaatcagg aataattgtt tgatgattaa ctgcagtcgt
    12661 attatcgcta gcaccaccag gtttcggatt gaggtgtaat atcacatcga gtgccttttt
    12721 caattgctct tcctctatat cgagcgattt taatagctta tcgtaatgtt tttttgtaaa
    12781 agcctcaaat tgtaaggtaa taatgcgcag tgccaatttt acatggctat tttgttcttt
    12841 gcgttctaat tgtatttgta agcactcttg caggttgcga gcaccaacac caggcggatc
    12901 gaagcgatga atagttcgca atacttcttc aatttcttca actgatgtcg atacattttt
    12961 ttgaaatgcc aaatcatcga caattgcatc taactctcta cgcaagtaac catcatcatc
    13021 aatgctacca atgagttggt cggctatttg ccattcgtgt tcatctaaat ctaataagtg
    13081 taactgctcg tctaaaaaat catgaaacgt tttgccgatg gcaagtggtg tttgtttttt
    13141 atcttcttta cctcgataat cgccgtcgtc attcgtataa tcataatcat ctttggtata
    13201 ttcttgaata tctaagctat cggcaaaatc atcatcttca ttattgtttt catagttttc
    13261 ctcgtcatcg cgtatttgtt ctggctccga taagtcttct ttatcattat caaaatccga
    13321 gatgctttct tccaatgctg gattaatttc caattcttct ttgatgcgtt gttctaaatt
    13381 tgcagtagga acttgcaata acttcatcag ctgtatctgt tgtggcgaca gcttttgtag
    13441 cattttctgt gatagacgtt gatgaagcat gtataaattg agaattaaaa attaaataat
    13501 aaaaatacgc aaacaattaa aactctgcac tttttggata acgtggaaat ggaattacgt
    13561 ctctaatatt tcccattccg gtaacgaaca atacgagcct ttcaaaccct aaaccaaaac
    13621 cagcatgtgg acatgttccg aattgacgtg tttccaaata ccaccaaata ctttcttctt
    13681 cgatgccaaa ttgatgtgcg cgtttcttca atttatcata gttttcttca cgttgtgaac
    13741 caccaataat ttcaccgatg cctggaaaca aaacgtccat agcacgaacg gttttaccat
    13801 cttcactcaa cttcatataa aatgctttga tgtgctcagg atagtccgtg agtataactg
    13861 gttttttata gtgtttttct actaaataac gttcgtgttc cgactgcaag tctgttcccc
    13921 aatcgacagg atattgaaat ttctttttcg cagttttcag aatttctatc gcttcagtgt
    13981 aagtcatgcg ctgaaattca ttttcaacac aaaatttcaa tttgtctaac aagcctaatt
    14041 cactgcgctc ttgcgttggt ttttgttttt cttcttcagc aaatcttttg tctaaaaatt
    14101 ccaaatcctc tttgcaatgg cttaatgcat agttaatcag atatttcaga aaatcttctg
    14161 ccaaatccat attatcgact aaatcattaa aagctacttc tggctcaatc atccaaaact
    14221 ccgctaaatg gcgagatgta ttagaatttt ctgctctaaa ggttggacca aatgtatata
    14281 tttttgacaa tgccattgca cctaattctc cttctaactg accagacacg gtaaggttgg
    14341 tttctttgcc aaaaaagtct tgtgaataat ctattttacc ttcttctgtt tttggtggat
    14401 tcgttgcgtc taatgtcgta acgcgaaaca tttcgccagc accttctgca tcagaacctg
    14461 tgataattgg tgtatgaaga taaaagaatc cttgatcatt aaaataatta tgaatcgcaa
    14521 atgctaacgc atgacgtatg cgaaggatgg cgccaaacgt atttgtgcgt ggtcttaaat
    14581 gcgctttttc tctcaaaaat tcgaatgaat gctttttagg ctgaatagga tatttttctg
    14641 catctgcttc ccctaaaatg ataatatttt ttaccgctac ttctactttt tgtgtgcctt
    14701 gcgaagctat caattgacct tgtatttcca aacatgcacc tgtatttatt tttgcaagta
    14761 aatcatcact aattgtttca gcttctacaa ctgcttgtat ggtgtgtatc gtcgtaccat
    14821 cgctgacatt aataaacgtt acgtttttac tgtttctttt cgtgcgaacc cagcctctaa
    14881 cgacaacatc acttcctacg gaagtatttt ctaaaatttc tttaatataa attttgcgca
    14941 taaatggaaa aattggattt caaaaatacg ctttttttag caaggtaaaa agtgtttaat
    15001 gcgcatttaa ccttagcaac acaaactata tatttactac aattaattct tatatttaca
    15061 gcaaataaca gtaatgagtt ttattcccgt attagatttg gctgattatt tatcaggcga
    15121 tgagacaaga agacagcgct ttattcaagg atttggaaaa gcctattcag aaattggttt
    15181 tgccgctatt gacaaccatg gcattccaca agctgttatc gataaatact atcatctcgt
    15241 agaacaattt tatgcattgc cattggaaag caaacgagga tacgaaaaag ttgaatacgc
    15301 tggacaacgt ggctacacat catttggtcg cgaaaaagcg aaacaaagcg aggtggctga
    15361 tttaaaagaa ttttggcaag tagggcaata tattgaagat ggcgaactta tgccagcagc
    15421 agattatcca gataatattg aagtagcaga actgcctgaa tttaatgaaa caggtagaca
    15481 actatataaa agttttgaaa atagtggccg tcatttactg cgtgcaattg cagaatattt
    15541 ggacttagat gtacattatt tcgatgctaa aatacacaat ggaaacagca ttttacgcgc
    15601 catacattat ccgccaatta cgcaagagcc taaatcagcc attcgtgcgg aagcgcatga
    15661 agacatcaac ttgattacgc tattggtcgg tgcaagtgct gatggtttgc aggtgatgga
    15721 cgtcgatggc aattggcacg atgctaaagc ggagccgcat caaataatgg tgaatgttgg
    15781 tgatatgtta caacgacttt gcaataacaa actcaaatca accacacatc gtgtcgtcaa
    15841 tccaccaaaa gagaaatggc atacacatcg ctattctatt ccatttttct tgcatccacg
    15901 aagtgaagta cgtttagatt gtttggaaaa ttgcattgac gcaacgcatc caattgcata
    15961 tgagcctatc acagcaggcg aatatttaga cgaacggttg agagaaatag gattgaaaaa
    16021 ataataaaaa taacaattag ctaattagct aattcattac attatgccat ctgctttatc
    16081 tataaaaata ggtgacttct tatataagaa tgcatttcct atttacaatg taatttatcc
    16141 gttttttaaa agaaaacaag acaaacacga aatagcacta ttaaaacaat atgtcaaaaa
    16201 aggagatatc gttttagata ttggcgctaa cattggtttc tacactaaaa tactcgctga
    16261 gcttgtaggt gaaaacggga aagtgtatgc ttttgaacca gataaaacca acttttcata
    16321 tttgatgaaa aatgctggac acttacggaa tgttgagttt cacaacaaag cagtaagtga
    16381 gaaaagcgga aaaattactt tatatcattc tgatttatta aatgtggacc ataaaacata
    16441 tgctacagaa aattacactt ccacttcaga gattgagtgt gtcgctatag atgatattat
    16501 tccaaatcac aaagtggatt ttataaaaat agatattcaa ggctacgaat attttgcctt
    16561 ccaaggtatg caaactgttt ttcaaaaaaa cgaaacacta aaaatcataa ccgagttgta
    16621 tccattaggt atgcaaaatg cgaaaattga tgctatcgca ttttttaatt tactttggga
    16681 aaataatttt atagtatata aaagtgaaga aaatagtagt tctgaattta ctataaatga
    16741 tattaagcaa tacacatcta taaaagatga atatacattt gtagatttat ttatagaaaa
    16801 gagaaattaa tatggcaaaa aaacaaccga ttagaaatca gcaacagcca gttgctacaa
    16861 ataaaaaaaa tacgacacgc actaccaatg ctatctctaa aagtatgttt gacattttag
    16921 aaactcattt ttctgctaag agaaatatgt atttgatttt attagtttta ataagtttta
    16981 tttttgcact tttttgtttt gacaataaaa tatctaccgc aaatgacgat gctttgtaca
    17041 ttgaatctgg cgcaagatat gccaatgcat tttttgcaaa aacaagttgg tacacagcta
    17101 atgcaccttt atatcctatt gttttaggtt taataattaa ggtaattgga gttaggttaa
    17161 tgtggttaaa aatgtttagt gtaatttcat ttgctgcggc aatatatttt atttttagaa
    17221 cgtatgaaaa acgaattcct tatttaattt taattccttc tttgctactt acagccatca
    17281 attttgaata cttgatgtat gctagtctaa cttatacaga aacattttca ctaatgataa
    17341 ttggcatttg tttcttctta atatttaaaa tctttgacaa attacaaatt gaaggtttat
    17401 ctatccaaga taaaatattg ccagtattta ttttaggtgc attatgtcta ttactaatgt
    17461 taacaagaag tgttgcttta ggtgctatcg gtgttgttgt actatatttg gtatatcgaa
    17521 aaaattataa agaagcagtt ttagccgttg caagttttgt aattgtattc cttgcctata
    17581 aatttttggt agatgccatc tggcagccaa gcgattctca atttaaaaca caaataaaat
    17641 taatgtatca aaaagatgta tataatccac aattaggaga tgaagatttg agtggtttta
    17701 tagtacgctt ttggaaaaac tgtgaaatat acatttcatc acgtttcatg tttgttttag
    17761 gatttagaga agaaatggct gagactaata cgcttgttac aataatatgc atcgctatag
    17821 ttttatggtc attgtattta gcacatatat ctaaacaata tac
//
LOCUS       DAOQQV010000048        17650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_992_length_17650_cov_6.591645, whole genome shotgun sequence.
ACCESSION   DAOQQV010000048 DAOQQV010000000
VERSION     DAOQQV010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17650
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_992_length_17650_cov_6.591645"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLL59_06530"
     CDS             <1..441
                     /locus_tag="PLL59_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23498.1"
                     /translation="AVALFAGAKFKETKWAIFVPIISLFISDAILAYINNFDVFHNTI
                     LFTYGSILLIILLGKLLNDNKINVGKTIAVTLSSSLLFFILSNFGTWMFGTMFTHDMS
                     GLIKCFIMAILFNKYSWHGDLFFVFAMFGIYETISNKIAITTKA"
     gene            570..1952
                     /gene="glmM"
                     /locus_tag="PLL59_06535"
     CDS             570..1952
                     /gene="glmM"
                     /locus_tag="PLL59_06535"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMY23499.1"
                     /translation="MTLIKSISGIRGTIGGKPGDALTPIDIAKFTLAFGTILKQKDAK
                     KIVVGRDARVSGEMVHHIIIGNLISIGLDVIDLGLATTPTVEMAVTAEQADGGIIITA
                     SHNPKQWNALKLLNNKGEFISEKVGQDVLKKANSSKFEFVDVDKLGQVTHKDYLDYHI
                     QKILEIPYVNVEAIQAKQFKVVVDAVNSVGGIAVPALLKALGVEAIHELYCEPNGKFA
                     HNPEPLPENLSALCSEVKQQKADVGIAVDPDVDRLALIDEKGIPFGEEYTLVSVADYV
                     LQHKPGNTVSNLSSTKALRDISRKHKVSYTASAVGEVNVVEKMKATNAVVGGEGNGGI
                     ILPELHYGRDALVGIALFLSHLATSNKSVHSLRAQYPNYVISKNKIQLEEGMDLHDII
                     EKIKLKYKQYPQNTIDGLRIDIDEDWVHLRGSNTEPIIRIYAESSTEQTANAIANKMQ
                     KDITTVLKEK"
     gene            2060..3061
                     /gene="holA"
                     /locus_tag="PLL59_06540"
     CDS             2060..3061
                     /gene="holA"
                     /locus_tag="PLL59_06540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HMY23500.1"
                     /translation="MASTLEAIWKNLHNKQYEPVYFLCGEEPYYIDLIEEFINNNALE
                     PHEKDFNFSMFYGKDADPIAVLNDAKQYPTFADKRLVIVREAQDLKVKDWELFEKYLE
                     QPVKSTILVFAHKNKTLDKRTKIAKIIEKKSVYFESKKIGEDALPNWIKGYVKKKNFT
                     IDDIAAKILAENIGNDLSRIANEMDKLSVMLSENAIITADIIEKYIGISKEYNITEFN
                     SAIIKRDFTKAMKMVLYFEKNPKAGPIIAILAFMYNHFSKLFVLHHIKDVKQATAAIG
                     WIPSDVSNGIKYYSIQKTQEILHLLCEYDAKAKGLYATGKNTNADLLKELVIKVIRN"
     gene            complement(3058..4209)
                     /locus_tag="PLL59_06545"
     CDS             complement(3058..4209)
                     /locus_tag="PLL59_06545"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HMY23501.1"
                     /translation="MTFLDANIFYFRFQTHLRFIYLHCFKYFTIRRFFIGILYALTIL
                     IVSILNIVTRLIDELIFPNYRNIHIKQPVFIISNPRSGTTFLHRLMCLDDNKFVYNLL
                     YHTTFPSITLYRIIQFFSTIDNKTGKNLFRLLEWINSKMFGGWDDIHATGFNKSEEDE
                     GLHFISGISASVGLVTPFLSEFKELYIPDKLDEKTKAYIKKYYKSTIQRWMYALGSDK
                     IFLSKTVMSSGRLEMLHEIFPDVKIIFLIRNPYQAIPSFTSMFAEPWSLLHPDIKEHS
                     EEYREWGELGIQYYKYFNEQKQNFKKENLITVAYTDLVAHPKETVIKIYEQLKLEITP
                     EFLARLEVATSTAKKYKSTHSYSLETYGFNKAHIYAELHFIFDEFGFEV"
     gene            complement(4287..4991)
                     /gene="mtgA"
                     /locus_tag="PLL59_06550"
     CDS             complement(4287..4991)
                     /gene="mtgA"
                     /locus_tag="PLL59_06550"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HMY23502.1"
                     /translation="MKNIFSVFWKWLKRIVAAFFILSIVSTILFRFIPVPITPLMLIR
                     CVEQIADGELPTLKKDWVPLSEINSSLVLAVIASEDQKFAEHFGFDFEAIEKVAKQNI
                     KLQKRGKPIKGGSTISQQCAKNVYLFPQRSYIRKAFEVYFTFLIEVFWSKKRIMEVYL
                     NVIEMGNGIYGAQAAAKTFFKKDAKQLSNAESATIAAVLPNPRRWNAGKPTGYINKRK
                     NWILRQMSHLRGSIQL"
     gene            5052..6416
                     /locus_tag="PLL59_06555"
     CDS             5052..6416
                     /locus_tag="PLL59_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HMY23503.1"
                     /translation="MLATKKLNQLPYPNSFHFFLGNLLQLRNERIQFFLEQKKALGNI
                     YQIKVPSRKIVVLTNPDWIKYVLVDNNKNYTKSFAYDSIKLFLGNGLLTSEGEFWKRQ
                     RRLSQPAFHKEKLSLMFKNMMAQTEKSIKRLEKYADTNLSINLSKELYKLTLDIVNST
                     LFYNEIDNTTDRIYKVVSKGNEYITKRIDSPIQLPEWLPTPSRMKEKEVLKEMDEIFF
                     SVIERRRKNPTQHEDLLSMLMDARDEETGEAMTNRQLRDEIITIFVAGHETTQIALGW
                     IFYLLTNNADVLQRLIHEIDSTLHGEAPTIQNIRSLHYLKQVIDESMRCFPPAWILGR
                     KTIEKDVIDGYEIPAGTNVIMPIYVVHHDEQYWENPSTFNPDRFTFENVKSQHKYAYF
                     PFGGGPRLCIGNNFAMQEMQVVIVMVLQKYRIIVNKDFIPDLNPLVTLHPKNEIFAKV
                     YKRK"
     gene            6467..7021
                     /locus_tag="PLL59_06560"
     CDS             6467..7021
                     /locus_tag="PLL59_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMY23504.1"
                     /translation="MAATETIRIPLGYTAPDFTLFDTVSDKFLSLQSLKGEKATVIMF
                     ICNHCPYVKHVNKQLVSLANDYISKGISFIAISSNDIVNYPQDAPELMKITANEEGYP
                     FPYLYDESQEVAKAYHAACTPDFSVFDANLSCVYRGQLDNSRPKNDSFSDGHDIRAAL
                     DNILAGIAVSEIQVPSLGCNIKWK"
     gene            7052..7954
                     /locus_tag="PLL59_06565"
     CDS             7052..7954
                     /locus_tag="PLL59_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23505.1"
                     /translation="MSILKKITENPLVESARAFTRATKTVNNLIALIADNKVSNSVRE
                     MLQGLRTVISEKPNITSVNHYINHFLLKIDPENQPIVIKELLEVFHERWKNVDRKTAE
                     IASQTFDFENKTILLLGNDINIQSLVDLLNVNQKKFNAIQLISFQKKFAKEQATLLAS
                     KGILVKVIDDAGLGKFLPEIDVILLGCDIIMHETFITNAGAHVIAAAAKHYDIPIFVL
                     ADSRKILNKKFFPQSVLASLIGSEEKSGDSIWKNAPENIEVVFNHIEEVPNHLITKFI
                     LEKESLTPSELPDKIDKVLVANFF"
     gene            8118..9068
                     /locus_tag="PLL59_06570"
     CDS             8118..9068
                     /locus_tag="PLL59_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23506.1"
                     /translation="MSHDIQIQQRKSNQQSFVITGIAMLAILITLLFIKLSRPIIDDA
                     ISGVMIDFGDSPDGLGDDNLVEAGGQSASPAPSVSNPTPPPPAPSTPIHQASTPVKAP
                     QNTTITAVDPQAVAIAQAAKEEKIRQQKALLEQQRIAELVKKAEQERLAREAEEARIK
                     AQTASVFTKGKTGQGNGSGNASGNGPGNGQGNTKPGGNQGAQWGTPGGSPNGTGNGPN
                     GSGPGSGNGMSYDLKGRTWRQKPLVFDNSQKTGKVVVGIKVDKNGNVVYAKYQQKGST
                     TTDAQLIKLAEDAALKSKFSADVGADEEQFGTITFKFSVQ"
     gene            9116..10399
                     /locus_tag="PLL59_06575"
     CDS             9116..10399
                     /locus_tag="PLL59_06575"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HMY23507.1"
                     /translation="MNYQQTIDYLFNKLPMFSRIGDAAIKKDLSNTIALCDFLQQPQN
                     KIKTIHVAGTNGKGSVSHMLASIFQEAGYKTGLYTSPHLKDFRERIKVNGKPCKEKYV
                     VKFTDKIKPIIEEIQPSFFEITVAMAFDYFAKQKVDIAIIETGLGGRLDSTNIITPIL
                     SVITNISLDHQHLLGDTLNKIAYEKAGIIKNNVPVVIGRKDAETDAVFIQKAADVNAA
                     LTFVDDVVSNLKYINTAKFLIVDATLQAKHVNVCCDLNSTYQVENIKTVLTTLSVIEQ
                     HQFFQIKEKAIQLGFSNVKQNTQLLGRWQTLDIEPKVIADVGHNEDGIKKIIAQLAHE
                     NYNKLHIVFGAVKDKDINKILNLLPKNAQYYFTEPQIPRKLAVEELYENAKTFDLVGN
                     IFHHPKDALGVAKSNAADNDLILICGSFFVVGEIL"
     gene            10492..11802
                     /locus_tag="PLL59_06580"
     CDS             10492..11802
                     /locus_tag="PLL59_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23508.1"
                     /translation="MRFQLFFISILLTFSSFATDKISTLAPKNYLVSYNLVKSHTTKT
                     ISALWKKHKIPKVILPVNNDVDIYEIIYKAKWIDSTWINCSGIYYAPKNIKKAAPIVM
                     YGHGTQLQKARKISDGDAQQGICLGFATDGYAACYPDYYGIGKGEKEHIYQHAWTEAM
                     SFIYMLYAVDELNKLIDVKTNGQLFLTGYSQGGHASFAAHKYLEELNDPRFQVTASSP
                     MSGAYDMTGEQEKYMFQVYPRPFYLPYLLVSYQRAYNIIDIDNIYSIFKPPFDTLLPK
                     FYADNQDRTLEDLDKIMPKIPAEVVVDSFVEVYKSTPNFFFKEKLKENNLTNWKPKAP
                     VQLCYCKGDREVNYKNSEVAYNTMKGLGVEHIKLNNLSDKLDHNTCAPFAVMATKYFF
                     DRFKNDGANPELKDVPIFKKKLADIVKKKEEKKYMKTGNDGIRY"
     gene            11869..12513
                     /locus_tag="PLL59_06585"
     CDS             11869..12513
                     /locus_tag="PLL59_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HMY23509.1"
                     /translation="MKIKICGMKYPENIQAVADLLPDYLGFIFYPKSKRFVLDEHKDA
                     FNSFMYDDNLRVQKVAVFVNESLKNIIRIATDYNIQIIQLHGDESSEFCEELQLLGFQ
                     VIKAFGVDADFSFASLDEYESVCDFFLFDTKTSDYGGTGQHFNWDLLINYHLQKPFFL
                     SGGLTINDLPKLKSLANRLPIHALDFNSKLEISPAMKDIEQCQLLINAIRNATK"
     gene            12590..13792
                     /gene="trpB"
                     /locus_tag="PLL59_06590"
     CDS             12590..13792
                     /gene="trpB"
                     /locus_tag="PLL59_06590"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HMY23510.1"
                     /translation="MSKIQSNSIFQVDEHGYYGQFGGAYIPEMLHPNIEELQQKYLDI
                     IYDESFQTEFLSLLKDYVGRPTPLYFAQRLSAHFKTQIYLKREDLCHTGAHKVNNTIG
                     QILLAKRLGKTRIIAETGAGQHGVATATVCALMNLPCVVYMGEVDIERQAPNVKRMQM
                     LGAEVRPAKSGSRTLKDATNEAMRDWIANPVDTHYIIGSVVGPHPFPDMVARFQSIIS
                     KEIKIQLKEKTGNENPNYVVACVGGGSNAAGAFYHYLAEQNVQLIAVEAAGHGVNSGK
                     SAATSQLGRVGVLHSSKTLFMQTEDGQVVEPHSISAGLDYPGIGPLHAHLFDTKRATF
                     LSATDEEALQAVLLLARLEGIIPALESAHAIAALDKLKAKENEIVVLNLSGRGDKDLD
                     TYIRELKL"
     gene            13816..14589
                     /gene="trpA"
                     /locus_tag="PLL59_06595"
     CDS             13816..14589
                     /gene="trpA"
                     /locus_tag="PLL59_06595"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HMY23511.1"
                     /translation="MNRINYLFQQKNKNVLSVFCTAGFPELHNIVPILEELQKNGVDM
                     VEIGMPFSDPTADGPVIQYSNTIAINNGMMLKVLFEQLKGVRAKIHIPLVLMGYLNPA
                     LQYGIDNFLNDAHACGIDGVIIPDLPMYEYETFYKEKFEKFDLQNIFLVTPQTTENRI
                     RKIDNLSNSFIYIVSSNAITGGNNDIQHAQEDYFKRIQQMQLKNPTVIGFGIKDKQTF
                     DTACNYANGAIIGTAFIKAIDQSKDLPTSIHHFIQTIKQ"
     gene            14619..15209
                     /gene="hisH"
                     /locus_tag="PLL59_06600"
     CDS             14619..15209
                     /gene="hisH"
                     /locus_tag="PLL59_06600"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HMY23512.1"
                     /translation="MKVVIIKYNAGNTKSVENALLRLQLQPIITDDADEIIRADKIIF
                     PGVGHAQTTMRYLKDRNLDVLIKSLKQPVLGICLGQQLMCAHSEEGDVDGLGIFPVNV
                     KKFISNNNCDKIPQIGWNSLYNVQSPLFASIPENSFVYFVHSYYCELSEYTIAKCDYI
                     LPFSAALQKDNFYAVQFHPEKSADVGQQIISNFISL"
     gene            15278..>17650
                     /locus_tag="PLL59_06605"
     CDS             15278..>17650
                     /locus_tag="PLL59_06605"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMY23513.1"
                     /translation="MIKYYFSVLFFVLCINISKASKNNETQMRRTCGTVMPSEQWKQE
                     FRQKVSDFKTQQNNSQNRINANYTLPIIVHVVYWNANENISAAQINAQLDVLNADYAG
                     TGFNVSNCPTAFQSLISNTNISFCPASKRPNGTTLPEVGIDRVNAVSAGFTNPGTSGW
                     SRSYIDNTIKPATTWDATKYLNVWVLPLDNTLLGYATFPGGPANKDGVVIGYTIYGMG
                     PSVSAPYDKGRTATHEVGHWLGVYHISGDEVCGDDECDDTPLQMGGNSDGENGLNYGC
                     PSFPFQVNGCGAGSSPNGELFMNFMDYVNDACMYLFTPDQRTRMQTTMTNFSMRSALA
                     SSNVCTVAPNIPVANFSANRTAVCPNSTVNFTDLSTGIPTSWSWSITPNTGWNYITGS
                     SASSQNPKVNFSTAGNYTVALTATNGLGADVETKNNYINVATPTGSSLPFAEGFQGSS
                     FPPSAWSLTSASTFNWERNTAVGGFGLSNACMYFNNYDNDADNKKDDINSPIINLNGA
                     INPRLKFDVAYAPYLEDNGTSRYDTLEVLITDFCANSTTSIYKKGGTQLATAPAKSLA
                     FAPNSAQWRIDSVNIPAAFLNKQIKITFRNYGKYANNIFIDNVNMYNVITSTCTGIPT
                     ANFTSSASSVCSGASLTFTNTSTSSSGTLDSIRWTNTSGTPNTSTAASYVPTYNTAGS
                     FTVTLKAYKCGNVNTKSQVITVNAKPNVTVTSPSICSGETATVQASGASTYSWSNGLG
                     TGNPKTTPILSSNTNYTVTGTDANNCTGTAVATITVKPKPTTPIITQGHDT"
BASE COUNT         6107 a         2883 c         3138 g         5522 t
ORIGIN      
        1 gcagtagcat tatttgcagg tgcaaaattt aaagaaacaa aatgggcgat ttttgtaccg
       61 ataatttctt tgttcatcag tgacgcgatt ttagcataca taaataattt tgacgtattt
      121 cacaatacga tattatttac gtatggttct atattgctga ttattttatt aggtaaatta
      181 ttaaacgata ataaaatcaa tgtaggtaaa actatagcag ttacactttc ctcttctttg
      241 ttgtttttta tactttctaa ttttggtact tggatgtttg gcaccatgtt cacacatgat
      301 atgtctggtt taatcaaatg ttttattatg gcaattctgt ttaacaaata tagctggcat
      361 ggcgatttgt ttttcgtgtt tgcaatgttt ggaatttacg agactatttc caataaaata
      421 gcaattacaa caaaagcata agaaatacta tagtagatta aactatttgt attattttct
      481 tatcgtaata ttatttaaca agtgttacta attttcaata acactcattg tacttattta
      541 cataaatttg acgctctaaa tttattgttt tgacattaat aaaatctata tcaggcattc
      601 gtggaacgat aggtggaaag ccaggtgatg ctttgactcc aattgatatt gcaaagttta
      661 ctttagcatt cggcactatt ctcaagcaaa aagacgcaaa aaaaatagta gtaggcagag
      721 atgctcgtgt gtctggtgaa atggtgcatc atatcatcat cggaaattta ataagcatcg
      781 gtttagatgt aattgatctt ggcttggcaa caacaccaac agtagaaatg gcggtaacag
      841 ctgaacaagc agatggtgga attattatta cggcatcaca caatccgaaa caatggaatg
      901 cgcttaaatt attgaataat aaaggcgagt ttatttcaga aaaagtgggt caagatgttt
      961 tgaaaaaagc aaattcttca aaatttgaat ttgtcgatgt agataaatta ggacaagtta
     1021 cacataaaga ttatctcgat tatcatattc aaaaaatact cgaaattcct tacgtaaatg
     1081 tcgaggcaat tcaagcaaag caatttaaag tggttgttga tgctgttaat agtgttggtg
     1141 gcattgccgt tccagcattg ttaaaagcat taggtgttga agctattcat gaattatact
     1201 gcgagccaaa cggaaaattt gcacacaatc cggagccatt accagaaaac ttatctgcac
     1261 tatgctctga agtaaaacaa caaaaagcag atgttggaat tgctgtcgac ccagatgttg
     1321 acaggttggc tttgatcgat gaaaaaggca ttccgttcgg cgaagaatat actttggtat
     1381 cggttgcaga ttatgtattg caacacaaac caggcaatac tgtatctaat ttgtcttcta
     1441 caaaagcatt gcgcgacatt agcagaaaac ataaggtcag ctatactgct tcagctgttg
     1501 gtgaagtaaa tgttgttgaa aaaatgaaag cgacaaatgc tgttgtaggc ggcgaaggaa
     1561 atggtggtat catcttacct gagttgcatt atggccgaga tgctttagtt ggtatcgcat
     1621 tatttttatc tcatttagct acatcaaata agagtgtaca ttcattgcgc gcgcagtatc
     1681 caaattatgt catttctaaa aataaaattc agttagaaga aggcatggat ttgcatgata
     1741 ttattgagaa aataaaatta aagtataagc aatatccaca aaacacaatt gatggtttgc
     1801 gtatcgatat agatgaagat tgggtgcacc tcagaggctc caatacggaa cctattattc
     1861 gtatttatgc tgaaagctca acagagcaaa cagcaaatgc aattgcaaat aaaatgcaaa
     1921 aagatatcac tactgtttta aaagaaaaat agactgttta tttttacttg taaatagtgg
     1981 aaatgtttta gtaaagcatt tacgctaata tttcataatt ctgtatcttt ccttttaatt
     2041 ctattttatc tcattttaga tggcaagcac actcgaagca atttggaaaa atttacataa
     2101 taaacagtat gaacctgtat atttcttgtg tggtgaagaa ccctattata ttgatttaat
     2161 tgaagaattc atcaataaca atgccttaga accacatgaa aaagatttta atttttcgat
     2221 gttttatggt aaagatgccg acccaattgc agtgcttaat gatgcaaaac aatatccaac
     2281 atttgcagat aaaagattgg tgatagtgcg cgaagcacaa gacctaaaag taaaagattg
     2341 ggagctcttt gaaaaatact tggaacagcc tgtaaaaagt acgattttag tatttgccca
     2401 caaaaataaa acgttagaca aacgtacaaa aattgcaaaa atcatcgaga aaaaatctgt
     2461 ctattttgag tcaaaaaaaa taggtgaaga tgctttgcct aattggataa aaggatatgt
     2521 gaaaaagaaa aatttcacga ttgatgatat agcagcaaag atactagcag aaaatattgg
     2581 aaatgactta tcgcgtattg ccaatgaaat ggataagctg tctgttatgt tgtctgagaa
     2641 tgcaataatt acggctgata tcattgaaaa atatatcggc atttcaaaag aatataatat
     2701 cacggagttt aatagcgcaa ttattaaacg cgattttact aaggccatga aaatggtctt
     2761 gtactttgaa aaaaatccga aagcaggacc aattatcgct attttggcat ttatgtataa
     2821 ccattttagc aaactatttg tattgcatca tataaaagat gtgaaacaag caacagcagc
     2881 tatcggctgg attccgtcag atgttagcaa tggcattaaa tactattcta ttcaaaaaac
     2941 gcaagaaata ttgcatcttt tatgtgagta cgatgctaaa gcaaaaggct tatacgcaac
     3001 aggtaaaaat accaatgctg atttattaaa agaattagtg ataaaggtaa ttcgcaatta
     3061 aacctcaaat ccaaactcgt cgaagataaa atgcaattct gcgtaaatat gtgctttatt
     3121 aaaaccgtat gtttctaatg aataactatg tgtgctttta tattttttgg cagttgaagt
     3181 agctacttcc aatcgtgcca aaaattcagg tgttatttct agttttaact gctcgtaaat
     3241 tttaattact gtttcttttg gatgcgctac caaatctgtg tatgcgacag taattaaatt
     3301 ctctttcttg aaattttgtt tctgctcgtt gaaatattta taatattgaa ttcctaactc
     3361 gccccattct ctatattcct ctgagtgctc tttaatatct ggatgtaata gactccaagg
     3421 ctctgcaaac atggaagtaa atgaaggtat ggcttgataa ggatttcgaa ttaaaaatat
     3481 aatttttaca tctggaaata tttcgtgtaa catctctaag cgtcctgaac tcattactgt
     3541 tttacttaaa aatattttat cgcttcctaa agcatacatc caacgttgta tcgtgctttt
     3601 ataatatttt ttaatgtatg cttttgtttt ttcatcgagc ttatctggaa tgtataattc
     3661 tttaaattcg cttaaaaaag gtgtaaccaa accaacacta gcagaaattc cggaaatgaa
     3721 atgcaagcct tcgtcttctt ccgatttgtt aaaaccagta gcatgtatgt cgtcccagcc
     3781 tccaaacatt ttggaattaa tccattctaa taaacgaaat aaattttttc cagttttatt
     3841 gtcaatggta ctaaaaaact gaataatacg atataatgtt atggaaggaa atgtagtatg
     3901 atagagtaaa ttatacacaa atttattatc atctaaacac atcaatcgat gcaagaatgt
     3961 tgtgccactt cttggattgc tgataataaa aacaggttgt ttgatatgta tgtttctgta
     4021 atttggaaat attaattcat ctattaatct cgtaacgata ttgagtatgc ttacgataag
     4081 tattgtcaat gcataaagta ttccaataaa aaaacggcgt attgtgaagt atttgaaaca
     4141 atgtaaatag ataaatcgta aatgcgtttg gaaacgaaaa taaaatatgt tggcgtctaa
     4201 aaatgtcatt tattgtgttg cgaggtgcaa aaatagcatt attcgtccat aaattaaggc
     4261 atacttgtgt taaggaaact caataattat aattgaatac tgcctcttaa atgactcatt
     4321 tgacgcaaaa tccaattttt ccttttgttg atatagccag taggcttgcc agcattccac
     4381 ctacgaggat tcggtaaaac ggctgcaatc gttgctgatt ctgcattgct aagttgtttg
     4441 gcatctttct tgaaaaatgt tttggcagca gcttgtgcac cataaatgcc atttcccatt
     4501 tctataacat ttagatagac ttccatgatg cgctttttac tccaaaatac ttcaatcaaa
     4561 aaggtgaaat atacttcaaa tgcttttctg atataacttc gctgaggaaa taaatacaca
     4621 ttttttgcac actgctgact aattgtgctg ccgcctttaa taggtttgcc tcgtttctgt
     4681 aattttatat tttgtttggc cactttttct atagcttcaa agtcaaaccc aaaatgctct
     4741 gcaaactttt gatcttcaga tgcaattaca gcaagcacca agctagaatt gatttcactt
     4801 aaaggcaccc aatctttttt taatgtaggt aactcaccat cagcaatttg ctctacacag
     4861 cgtatgagca tcaatggtgt gatgggcaca ggtataaatc gaaataaaat agtggaaaca
     4921 atactgagta taaaaaatgc ggcaacgatt cgtttaagcc atttccagaa aacgctaaat
     4981 atatttttca tcttttgtta atccaaagat gagaaacttc atgtaatttc agaaaaataa
     5041 atatccactt tatgttagcc acgaagaagc tgaatcagtt gccttatccg aattcctttc
     5101 acttttttct tggcaatttg ttacagctga gaaatgaacg cattcaattt tttttggaac
     5161 aaaaaaaagc gcttggcaat atttatcaaa tcaaagtgcc atcgcgaaaa attgtagtgc
     5221 ttacaaatcc tgattggata aaatatgtat tggtcgataa taataaaaac tataccaaaa
     5281 gctttgctta tgattcgata aagttgtttt taggaaatgg tctattaaca agtgaaggtg
     5341 aattttggaa acggcagcga aggttgtcgc aaccagcatt tcataaggag aaattgtcct
     5401 tgatgtttaa aaacatgatg gcgcaaacag aaaaatctat aaagcgatta gaaaaatatg
     5461 ccgacactaa tctttccata aatttgtcta aagaattata taaactcacc ttggatattg
     5521 tcaattctac attattctac aatgaaattg ataatacaac ggatagaatt tataaggtag
     5581 tatcaaaagg caatgaatac atcacgaaaa gaattgatag tcctatacaa ttgccagaat
     5641 ggctgcctac tccatcgcga atgaaagaaa aagaagtatt gaaagagatg gacgaaatat
     5701 ttttttccgt cattgagcga aggcgaaaaa atccaacaca acacgaagat ttattgtcga
     5761 tgcttatgga cgcgagagac gaagaaacag gagaggcaat gacgaaccgt caattacgcg
     5821 atgaaatcat taccattttt gtagcaggtc atgaaacaac acaaattgca ttaggctgga
     5881 ttttctattt gcttacaaat aatgctgatg ttttgcaacg tttaatacat gaaatagata
     5941 gtacattgca tggtgaagca ccaacaattc aaaatattag aagtttgcat tatctaaaac
     6001 aagttatcga tgagtcgatg cgttgttttc caccagcttg gattctaggt cgtaaaacga
     6061 tagagaaaga tgtcattgat ggctatgaaa ttcctgcagg cactaatgtt attatgccta
     6121 tttatgtcgt gcatcacgat gaacaatatt gggaaaatcc atcgacattt aatccagata
     6181 gatttacttt tgaaaatgtg aaatcacaac ataagtatgc ctatttccca tttggtggtg
     6241 gcccaagatt gtgtattggt aataattttg ccatgcaaga aatgcaagtt gtaattgtca
     6301 tggttttgca gaaatataga attatagtca ataaagattt tattccagat ttaaacccac
     6361 ttgtgacctt gcatcctaaa aatgaaattt ttgctaaagt ctataaaaga aaataatgta
     6421 taaatttata tgcctacatt taccataatt aaatagatag atacacatgg cagcaacaga
     6481 aaccatcaga attcctttag gctatacggc acccgacttt actttatttg atactgtatc
     6541 tgataaattt ttgtctttac aatcgttaaa aggagagaag gcgaccgtga taatgttcat
     6601 ttgcaatcat tgtccgtatg taaaacatgt caataaacaa ttagtgagct tggcaaatga
     6661 ttatatatca aaaggaatat cttttattgc tattagctct aatgatattg tcaattatcc
     6721 gcaagatgca ccagaattaa tgaaaataac tgcaaacgaa gaaggctatc catttcctta
     6781 tttgtacgat gaatcacagg aagtcgcaaa ggcatatcat gccgcatgca cgccggattt
     6841 tagtgttttt gatgcaaatt tatcatgtgt atatcgcggc cagcttgaca actcaagacc
     6901 aaaaaatgat agtttctctg atggacatga tatacgtgct gctttagata atattttggc
     6961 aggcattgcc gtaagtgaaa ttcaagtgcc aagtcttggc tgtaatatta aatggaaata
     7021 aatcagatac actcagcaat aaaccttaca tatgagtatt ttaaaaaaga taacagaaaa
     7081 tccattggtc gaaagtgcac gtgcatttac acgcgctact aaaaccgtca ataatctgat
     7141 tgcactcatt gccgacaata aagtatcaaa ttctgttcgt gaaatgctac aaggtttgcg
     7201 aacagttatt tcagaaaaac caaatattac gtctgtcaat cactatatca atcatttttt
     7261 attaaaaatt gatcctgaaa atcagccgat agtcatcaaa gaattattag aagtgtttca
     7321 tgaacgctgg aagaatgtgg acagaaaaac agcagagatt gccagccaaa catttgactt
     7381 tgaaaataag actattttgc tacttggcaa tgatatcaat attcaatctt tagttgattt
     7441 gttaaatgtt aatcagaaga aattcaatgc catacaatta atttcatttc aaaagaaatt
     7501 tgctaaagaa caagcaacgc tattagcatc aaaaggaatt ttagtaaaag taatagacga
     7561 tgctggttta ggaaaattct tgccagaaat agatgttatt ctcttaggtt gtgatattat
     7621 catgcatgaa acattcatta ctaatgctgg cgcacatgtc atcgcagcag ctgcgaagca
     7681 ttatgacatt cccatttttg tattagcaga tagtcgcaaa attttgaata aaaaattttt
     7741 ccctcaatca gtattagctt ccttaattgg aagtgaagaa aaatctggcg atagcatttg
     7801 gaaaaatgca cctgaaaata tagaagtagt atttaatcac attgaagaag tacccaatca
     7861 cttaattaca aagtttattt tagaaaaaga atcactaact ccaagcgaat tgcctgataa
     7921 aattgacaaa gtgctcgttg ctaacttttt ttagcattca ttagttattg ttttaaacaa
     7981 taaatttatc taaaggaagt taaattaata gtcttggcat ttattttgat ttaataacag
     8041 tggtgtgtat tttttttgct gcataaaata ataaacacgt aattttcagc gcaaataagc
     8101 tcatatatag aataacaatg tctcacgata tacaaataca acaacgaaaa tccaaccagc
     8161 aatcttttgt tattacaggt attgccatgt tggctatatt aattacctta ttatttatca
     8221 aattgagtcg cccaattatt gatgatgcaa tttctggcgt tatgattgat tttggcgata
     8281 gtcctgatgg cttaggcgat gataatttag tagaggcagg cggacaaagt gcttctccag
     8341 ctcctagtgt ttccaatcca acgccaccac ctccagcgcc atctacacca attcatcaag
     8401 cttctacgcc agtaaaagcg ccacagaata caactattac tgctgtcgat ccacaagcag
     8461 tggcaattgc acaagcggca aaagaagaga aaatcagaca acaaaaagca ttattagagc
     8521 aacaacgtat tgcagagttg gtgaagaaag cagaacaaga acgacttgca cgtgaggcgg
     8581 aagaagcacg tattaaagcg caaacggcta gtgtgtttac gaaaggcaaa acaggacaag
     8641 gcaatggtag cggaaatgcg agtggtaatg gaccaggcaa tggtcaagga aatactaaac
     8701 caggcggaaa ccaaggtgca caatggggaa cacctggcgg ctctcctaat ggtactggaa
     8761 acggtcctaa tggcagtggc cctggtagtg gaaatggtat gagctacgac ctaaaaggta
     8821 gaacatggcg ccaaaaacct ttagtttttg ataattctca aaaaacagga aaagtcgtgg
     8881 taggtattaa agttgacaaa aatggtaatg ttgtttatgc taaatatcaa caaaaaggaa
     8941 gcacaacaac agatgcgcaa ttaatcaaat tagcggaaga tgcagcattg aaatctaaat
     9001 ttagtgctga tgttggtgct gatgaagaac aatttggtac gatcactttt aaatttagtg
     9061 ttcagtaatc gcttatttta ttttatgttt gcaaacattt agtaagcatt agtgaatgaa
     9121 ttatcaacaa acgattgact atctttttaa taagttgcct atgttttcgc gcattggcga
     9181 tgctgcaata aaaaaagatt taagcaacac gattgcttta tgcgattttt tacaacaacc
     9241 acaaaacaag ataaaaacaa ttcatgtagc aggtacaaat ggcaaaggtt ctgtatcgca
     9301 catgttggct tcaatttttc aagaagctgg ctataaaaca ggattgtaca cgtctcctca
     9361 tttaaaagat tttagagagc gaattaaggt taatggaaaa ccatgcaaag agaagtatgt
     9421 ggtaaaattt acagataaaa tcaagcccat aattgaagaa attcagccat cattttttga
     9481 aattacggta gcgatggcat ttgattattt cgcaaaacaa aaagtggata tagctatcat
     9541 cgaaacaggt ttaggtggcc gattagactc aacgaatatc attacgccaa ttctttctgt
     9601 cattaccaat attagcttag atcatcaaca tttattaggc gatacgttga ataagattgc
     9661 ctatgaaaaa gcaggaatta taaaaaacaa tgtgcctgta gttattggca gaaaagatgc
     9721 tgaaacggac gctgttttta ttcagaaggc tgctgatgta aatgctgctt tgacttttgt
     9781 tgatgatgtt gtaagcaatt tgaaatatat aaatacggca aaatttctga ttgtagatgc
     9841 cacattgcaa gctaaacacg ttaatgtttg ttgtgattta aattctacct atcaagtaga
     9901 aaatataaaa actgttttga ctactttgtc agtaatagaa caacatcaat tttttcaaat
     9961 caaagagaaa gccattcaat tagggttttc caatgttaaa caaaacacac aacttcttgg
    10021 caggtggcaa acattggata tagaacccaa agtaattgca gatgttggtc ataacgaaga
    10081 tggaattaaa aaaattatag cacaattggc acatgaaaac tataataagt tacacatagt
    10141 ttttggtgcc gtaaaagaca aagacatcaa taagatattg aatctactgc cgaagaatgc
    10201 acaatactat tttacagagc cacaaattcc acgcaaatta gccgtcgaag aattatatga
    10261 aaatgcaaaa actttcgatt tagttggcaa tattttccac catccgaaag acgctttagg
    10321 cgtcgctaaa tctaatgctg ccgacaatga tttgattcta atttgtggaa gtttttttgt
    10381 tgttggtgaa atcttgtaat tttttttgac aaaaatcatc aaaagtttaa ataaagttaa
    10441 tcgacttgta agtaactaaa aacagttata tcttgttgaa aaattctatt catgcgtttc
    10501 caattatttt tcatctctat cttactcacg ttttcatcat ttgctactga taaaattagc
    10561 acattggcgc caaaaaatta tttggtaagc tataacttag taaaatcaca cacgacaaaa
    10621 acgatttccg ctttatggaa aaagcataaa attccaaaag tgattttacc agtaaacaac
    10681 gatgtagata tttatgaaat tatatataaa gcaaaatgga tagacagcac ttggatcaat
    10741 tgctcaggta tttattatgc accaaaaaat ataaaaaaag cagcaccaat tgtgatgtat
    10801 ggacatggaa ctcagctgca aaaggcaaga aaaataagtg atggcgatgc acaacaaggc
    10861 atttgcttag gttttgctac tgatggttat gctgcttgct atcctgatta ttatggcatt
    10921 ggcaaaggtg aaaaagaaca tatttaccaa catgcatgga cagaagcaat gtcgtttatc
    10981 tatatgttgt atgctgttga tgaattgaat aaattaattg atgtaaaaac aaatggtcaa
    11041 ttgtttttaa caggctattc tcaaggtggt catgcttcct ttgctgctca caaatacttg
    11101 gaagaactaa acgatcctcg ttttcaagta acggcttcat cgccaatgag tggtgcttat
    11161 gatatgactg gcgaacaaga aaaatatatg ttccaagtgt atcctcgacc attttatttg
    11221 ccttatttgt tagtgtctta tcaacgagct tataacataa tagacattga caatatttat
    11281 agtattttca aaccgccttt tgatacttta ttacctaaat tttatgcaga caatcaagat
    11341 agaacattag aagacttaga taaaattatg cctaaaattc cggcagaagt agtggtcgat
    11401 tcttttgttg aagtatataa atcaacacca aatttcttct ttaaagaaaa attaaaagaa
    11461 aataatttga caaattggaa accaaaagct ccagtacaat tatgctattg caaaggcgac
    11521 agagaagtga actataaaaa ctcggaagtt gcttataata cgatgaaagg cttaggtgtt
    11581 gaacatatca aattaaataa tttaagtgat aaactcgacc acaacacttg tgctcccttt
    11641 gctgtaatgg ctacgaaata tttctttgat agatttaaga atgatggcgc aaatcctgaa
    11701 ttaaaagatg ttcctatttt caagaaaaaa ttggctgaca ttgtgaaaaa gaaagaagag
    11761 aaaaaatata tgaaaacagg taatgacggc attcgttatt aattgttttg ttcttactac
    11821 ttactcaagg cttgaaaatt gtgcatattt ttttcttatt tttactagat gaaaattaaa
    11881 atatgtggca tgaaatatcc tgaaaacatt caggctgttg ccgatttatt gcctgattat
    11941 ttaggtttta ttttctatcc aaagtctaaa agatttgtac ttgatgaaca taaagacgca
    12001 tttaattcat ttatgtatga tgataacttg cgtgtacaaa aagtagcagt atttgtcaat
    12061 gaatctttaa aaaacatcat tcgaattgcc acagattaca acattcaaat aatacaatta
    12121 cacggtgacg aaagttcaga attttgtgaa gaattacaac tcttaggctt tcaagtaatc
    12181 aaagcatttg gtgttgatgc tgatttttca tttgcctcat tagatgaata tgaaagtgtt
    12241 tgcgatttct ttttattcga tactaaaacc agcgattacg gtggaacagg acagcatttt
    12301 aattgggatt tattaatcaa ttaccatttg caaaaacctt ttttcctatc tggtggtttg
    12361 actatcaatg accttccaaa gttaaaatca ttagccaatc gtttaccaat tcatgcactt
    12421 gattttaata gcaaattaga aatttcacca gcaatgaaag atattgaaca atgtcaattg
    12481 ctgataaatg ctatacgtaa tgctacaaag taacattgta ttaacgaact aacttttcaa
    12541 tcattcaatt ttattactac attgtacttt tatttttatg gaaaattcga tgtcaaaaat
    12601 acaatcaaat tcaatatttc aagtcgatga acatggctac tatggtcagt ttggtggtgc
    12661 atatattcct gaaatgttac atcctaatat cgaggaacta caacaaaaat atttggatat
    12721 tatttacgac gaaagtttcc aaacagaatt tttatcgctt ttaaaagatt atgttggacg
    12781 accaacacca ttgtattttg cccaacgatt gtctgctcat tttaagacgc aaatttatct
    12841 taaacgcgaa gatttatgtc acactggtgc gcacaaagtg aacaatacaa taggtcaaat
    12901 tttattggcc aaacgattag gcaaaactag aataatagct gaaactggtg caggtcaaca
    12961 tggtgttgct actgctactg tttgcgcctt aatgaatttg ccatgtgtcg tctatatggg
    13021 tgaagttgac atcgaaagac aagcacccaa tgtcaagcgt atgcaaatgc ttggtgcgga
    13081 agtgcgtcct gcaaaatcag gctcacgcac gctgaaagat gctacgaatg aagccatgcg
    13141 cgattggatt gccaatcctg tcgacacaca ttatatcatt ggtagcgtag tgggtccaca
    13201 tccatttcca gacatggttg cacgttttca aagtattatt tctaaagaaa taaaaataca
    13261 actgaaagaa aaaacaggca acgaaaatcc gaattatgtt gtcgcttgcg taggtggtgg
    13321 gagcaatgcc gctggtgctt tttatcatta tttagccgaa caaaatgtac aattgattgc
    13381 agtggaagct gctggacacg gcgtgaactc aggcaagagc gcagcaacat cacagctcgg
    13441 tagagtaggc gtgttacata gtagcaaaac gctttttatg caaacagaag atggtcaggt
    13501 tgtcgagcct cattcaattt cagcaggctt agattatcca ggaattggtc cgttacacgc
    13561 acatcttttt gataccaaac gtgccacttt tttgagcgcc actgacgaag aagccttgca
    13621 agccgtactt ttattagcgc gattagaagg tataattcct gctttagaaa gtgcacatgc
    13681 tattgctgca ttggataaat taaaggcaaa agaaaatgaa attgttgtgc ttaatctttc
    13741 tggtcgtggc gacaaagatt tggatacata catcagagaa ctaaaattgt agtgaaataa
    13801 ttttataact aaatcatgaa cagaataaat tatttattcc aacaaaaaaa caaaaatgta
    13861 cttagtgttt tctgcacagc tggatttcct gaattacata atattgttcc aattttagaa
    13921 gaattacaaa agaatggtgt ggatatggta gaaataggaa tgccattttc tgatccaaca
    13981 gctgatggtc ctgtgattca atattccaac acaatagcta ttaacaatgg aatgatgcta
    14041 aaagtattgt ttgaacaatt aaaaggtgtt cgagcaaaaa tacacattcc tttggtgttg
    14101 atgggttact taaatccagc attacaatat ggtatagata attttttaaa cgatgctcat
    14161 gcttgtggaa ttgatggtgt cattattcct gatttgccaa tgtatgaata tgaaactttt
    14221 tataaagaaa aatttgaaaa attcgatttg caaaatatat tcttagttac tccacaaaca
    14281 actgaaaaca gaattagaaa aattgataat ctctctaatt cttttattta tatcgtttct
    14341 tccaatgcga taacaggtgg caataatgac attcaacatg cacaagaaga ttatttcaaa
    14401 cgcatccaac aaatgcaatt aaaaaatcca actgtaattg gatttggtat caaggataaa
    14461 caaacatttg atactgcctg taattatgct aatggtgcaa ttattggtac tgcatttatt
    14521 aaggcaatag accaaagtaa agatttgcca acatcaatac atcattttat tcaaacaatc
    14581 aaacagtaat tttttttcaa agacgaagta tcttttttat gaaagttgtc atcatcaagt
    14641 ataatgctgg aaataccaaa agcgtagaaa atgctttact acgcttgcaa ttgcagccta
    14701 taataacaga tgatgctgat gagattatta gagcagataa aataatattt cctggtgttg
    14761 gtcatgcaca aacaaccatg cgatatttga aagatcggaa tttagatgtg cttatcaaaa
    14821 gtttaaaaca accagtatta ggaatttgcc ttggccaaca attaatgtgc gcacacagcg
    14881 aagaaggtga tgtagatggc ttaggaattt ttcctgtgaa tgtaaaaaag ttcatttcta
    14941 ataacaattg cgataaaata cctcaaatcg gttggaacag tttatataat gtccaatctc
    15001 ctttgtttgc ttctattcct gagaatagtt tcgtgtattt tgtacatagt tattattgtg
    15061 aattaagcga atataccatt gcaaaatgtg attatatctt accattttct gcagcattgc
    15121 aaaaagataa tttttatgca gtacaatttc atccagaaaa aagtgctgac gttggacagc
    15181 aaataatcag taattttata agcctctgag gtttattggt tttcattttt ttaaaaaaaa
    15241 tcgttcattt tctgtatatt tgaatataaa ttcaactatg ataaagtatt atttcagtgt
    15301 gcttttcttt gttttatgta taaacatttc aaaagcatca aaaaataatg aaacgcaaat
    15361 gcgtcgtact tgtggtactg taatgccttc agaacaatgg aaacaagaat ttcgccaaaa
    15421 agtaagcgat tttaaaacac aacaaaacaa tagtcaaaac agaataaacg ctaattacac
    15481 tttaccaatt attgttcatg ttgtgtattg gaatgccaat gaaaatattt cagcagcaca
    15541 aattaatgct cagctcgatg tattgaatgc agattatgct ggtacaggtt ttaatgtttc
    15601 aaattgtcct actgcctttc aatctcttat ttcaaataca aatatttcct tttgtccagc
    15661 ttcaaaacgt ccaaatggta cgaccttgcc tgaggtaggc atcgaccgag tgaatgcagt
    15721 ttcagctgga tttaccaatc ctggcaccag tggttggtca agaagttata ttgataatac
    15781 catcaagcct gctacaactt gggatgctac aaaatattta aatgtttggg tattgccatt
    15841 agataataca ctcttaggtt atgccacatt tccaggaggg ccagccaata aagatggtgt
    15901 agtaataggc tatactattt atggcatggg accaagcgta agtgcacctt acgataaagg
    15961 taggacggca actcatgaag taggccactg gctcggtgtt tatcatatta gtggcgacga
    16021 agtgtgtggc gatgatgaat gtgacgacac gccactacaa atgggcggca atagtgatgg
    16081 tgaaaatgga ttaaattatg gctgtccctc ttttccattt caagtgaatg gctgcggtgc
    16141 tggctctagc cctaatggtg agttgtttat gaattttatg gattatgtca atgatgcctg
    16201 tatgtatctt tttacgccag accagagaac tcgtatgcaa acaaccatga caaacttttc
    16261 aatgcgaagc gctttagctt catctaatgt ttgtactgtt gcaccaaata ttcctgtggc
    16321 taatttttca gcgaacagaa ctgctgtttg tccaaatagt acggttaatt ttacagattt
    16381 atctacaggt attcctacat catggtcgtg gtcaatcacg cccaatacgg gttggaatta
    16441 tataacaggt tcatctgcaa gttcacaaaa tccaaaagtg aattttagta ctgctggaaa
    16501 ttatactgtt gctttaactg ctactaatgg cttaggcgca gatgtggaaa caaaaaataa
    16561 ttatatcaat gttgctacgc caactggtag tagtttgcct tttgcagaag gttttcaagg
    16621 aagctcattt ccaccttcag cttggtcgct aacaagtgct tctactttta attgggaaag
    16681 aaacacggca gttggcggct ttggtctgtc gaatgcttgt atgtatttta ataattacga
    16741 taatgatgcc gacaataaaa aagatgatat caattctcca attataaatc ttaacggcgc
    16801 aataaatcca cgcttaaaat ttgatgttgc ctatgcgccg tatttagaag ataatggcac
    16861 aagcagatat gatacattag aagttttaat tacagatttt tgtgctaatt caaccaccag
    16921 catttacaaa aaaggcggca cgcagttagc aacagctcca gctaaaagcc ttgcttttgc
    16981 acctaattcg gctcaatggc gtattgactc agtaaatatt ccagctgcct ttttgaataa
    17041 acaaataaaa attactttta gaaattatgg caaatatgcc aacaatattt ttatagataa
    17101 tgtaaatatg tacaatgtaa ttacaagtac atgtactggc atacctactg cgaacttcac
    17161 aagctctgct tcatcggttt gtagtggcgc atcgcttaca tttacaaata cttcaacatc
    17221 ttcgtctggt acgctcgatt ctattcgttg gacaaacaca agtggcacac caaacacatc
    17281 aacagctgct tcatacgtgc caacatataa cacagctggc tcatttactg tcacactaaa
    17341 ggcttataaa tgtggcaatg taaacacaaa atctcaggtc ataactgtaa acgcaaaacc
    17401 aaatgttacg gtaacttctc ctagtatatg tagtggagaa acagcaacag ttcaagcctc
    17461 tggtgcgagc acatatagct ggtcgaatgg tcttggaaca ggcaatccaa aaactacacc
    17521 tatactaagt agtaatacta attatacggt tacaggaact gatgcgaata actgtactgg
    17581 cactgctgtt gcaacaatta cagttaaacc aaaaccaact acaccaataa ttactcaagg
    17641 ccacgatact
//
LOCUS       DAOQQV010000049        17624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_995_length_17624_cov_7.298879, whole genome shotgun sequence.
ACCESSION   DAOQQV010000049 DAOQQV010000000
VERSION     DAOQQV010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17624
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_995_length_17624_cov_7.298879"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLL59_06610"
     CDS             complement(<1..699)
                     /locus_tag="PLL59_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HMY23514.1"
                     /translation="MQAIFLIKNGPSKQAFEFRNINMSEPNDDEVGIIVEASGINFAD
                     VTARLGQYRDCPPLPTIVGYEAVGRVEKVGKKVTSVKKGDRVLVFTRFGGYSQYVIQK
                     ENAIAKIGEDMDAGKALALATQYSTAFYACSVATNVLPHEKVLIHAGAGGVGTALIQL
                     CKLKNAKIYTTAGSEKKLAYCKEQGADVSINYTKEDFTQVIKEPIDVAFDSLGADNFR
                     KSYNLLQRGGRIVGY"
     gene            complement(717..1949)
                     /locus_tag="PLL59_06615"
     CDS             complement(717..1949)
                     /locus_tag="PLL59_06615"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HMY23515.1"
                     /translation="MRLLQSLLLVLFLMMNVTFVRANTKTYTFNSNKSKISIPFKLYN
                     NIIIVDVKINETKTLKFIFDSGCKSTIVIHPKWIDSFEIPLHQKVFFSGLGIRDSIET
                     RKMENGRIDIDLIRGENIPIFFLTKDTLNLDHYLGTDVDGIFGAELFERYYIFINYKK
                     RMIDFYHKKPVKKINSTFAKLPVQIRKSKGYINCAIMNSNNEIFMSELLLDTGSNIPI
                     IIKNKVPSDLNLNQYIQAEIGEGLSGAMYSSVGRIKRLFIDTLRFDSVIIAFNETPIT
                     FKEPDEYTLDGNIGNDILNRMDIYFAYPEKYVYFKPNRNIHDKFEFNISNIILLENKT
                     KNGGFIIKSIAENSPPLMAGLQQGDEIIKIDTYKCEDIQLEDALRLLNKRIGKKINIQ
                     YKRNGISNKISYKLSSII"
     gene            1996..3849
                     /locus_tag="PLL59_06620"
     CDS             1996..3849
                     /locus_tag="PLL59_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMY23516.1"
                     /translation="MNYLTVNNLCKYYGDKLLFENITFFIDKGQKVALIAKNGTGKSS
                     LIKILQGLEPPESGDYKFHKDISAGFLVQEPDFDDNKSVYQTIMHADNPVLMASLNYE
                     YALDGHGDLQMAMNKMDELQAWDYEHKMKLILSKFLIFDLDQKVGTMSGGQKKRLALA
                     ILLLEEPDIFILDEPTNHLDMDMIEWLEDYLTKNNITLLMITHDRYFLENICTEILEL
                     DNGILYKHKGNYSKYLENKELREENQRINVEKAQNTFRKELEWMRRQPKARTTKAQSR
                     VDNFENVKAAATAKAEDKRVEIEVKMQRLGGKILELHNISKSHGDKKLLHKFSHKFQR
                     GERVGIVGRNGCGKSTLLNIITGTELQDDGNIVLGETVVFAYYNQKGLQLKVDKKVID
                     VIKDIAEYLPIGKGTLSASQLLERFLFVPSQQHNLVSTLSGGERRRLYLLTILMKNPN
                     FLILDEPTNDLDILTLQVLEDFLLDFKGCLLIVSHDRYFLDKLTEHLFVFEGEGVVRD
                     FLGNYNEYRLKLKDEQREAARAEAQQQTNTTKPTANSNKRKLTFKEQTEFKQLEQDID
                     TLSNEKSALTAQLSGGMLHHDEIQKIAKRLEEIILYLDAKENRWLELSEFV"
     gene            3867..5033
                     /locus_tag="PLL59_06625"
     CDS             3867..5033
                     /locus_tag="PLL59_06625"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMY23517.1"
                     /translation="MTKKYFHNFNGLRFYFASYILFAHIEEIKWVLKLPNFFHDFKFV
                     EPLGTSAITLFFTMSGFLISYFLFLEKDKNEDSKINLKKFYKSRVVRIWPLYFLCIVL
                     YWFIGPHSVLKELFNSIYFTQVLKTPFTTDIPHAVYLGLFLIILPQLAGAIAFTYGGM
                     AIYGGHAWSIGSEELFYLFWPLLINKFVSYKKIFKVGFIAVYSCYGLVILAFIVNKLF
                     FKYTPLNGVLLTITSFLALTRLYCMLIGGLLAYLFVNNHRWLSFLRKKSVLYTAVVML
                     FIMIANGLDAPMLIHDIYALLWSVVLLYFADNESGHKWLNNKVVSYLGSITYGVYMYH
                     VFVIIVLLYFAQQLNITHDFWLFNIFLYVLSPLLTFTVSHYSYKYFENWFFRFK"
     gene            5051..6610
                     /locus_tag="PLL59_06630"
     CDS             5051..6610
                     /locus_tag="PLL59_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23518.1"
                     /translation="MNNDNIKKYALLLISIFSFFLGVLIFFQVYIDDAFIFFKYGYNL
                     VNHGIWSWSIEGKPAEAYTSFIYAVLSIFPALLHVPPHIFIKVVGLLFLLALLFRVYS
                     KISNKKWALIAMLVITTNWQLYTHAFSGLETVLWLWLLLESFFLIEKEELTVKNQMLL
                     WSICLLMTLTRPEGAIYGFFFFVYLVFYKKQKLHFGVLATLIFIGILYFAARFAYFGL
                     PFPLPFYHKLMDSRFAKLAWIFNIYTSWYYIICATLVLYFFRKHKVIYFIGIITFLIF
                     IGLYLRAFLVMNFADRFPFQLFWPFILFALIKLEKSTYVDKVKIVLIACFLNMIIFSK
                     GLYDENLIELASITNNAGAAFFLPRSHYILAKNINKIKDVERKKLKVLFGDAGMFPYY
                     VKAQCYDYNGLVDAEIAINKLDKKYFYSKQADIVLIGTPNENKIGLKQDLSNCKTIYE
                     YAESDSTYKYVGKVISKVNGYYVHIYFNTKSSYAKDVETALNNAVTESANAQFRIKRF
                     LKLKYLDLENI"
     gene            complement(6621..7811)
                     /locus_tag="PLL59_06635"
     CDS             complement(6621..7811)
                     /locus_tag="PLL59_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMY23519.1"
                     /translation="MTFKKMRLVWLITLLPILSSCDAVLQNLQKSHQQQTASSLNAGT
                     APKRAYTLAFPPNKDRILPDGYANSGDMLVTTPNPWGIRYKDYIFNDFDIHTFYYKHD
                     APMWDNTKKDLIIDVITPRNDAEKKRPCIIFLFGGGFAGKFDDCTQEIGKGFAQKGYV
                     VALPDYRIGYKNALLAGQCVGDFQTGMYPAYMRGVQDVRSAIRYLKANADKFGIDKNK
                     IFVSGHSAGALATIGLAVQTTENAPKNVLEQVGGSLDPMNDNMNYDATVAGVIALAGA
                     VADPNVIVGKKINTPMHLIHGTCDELIDMYAGNPFRCKERKTFPIVYGGAAIYEAAKL
                     NNDNVSLSVICNGSHSMSSIGYNELIDIMNDFTYGIISGNPIKGKKIIPAKKGICNKP
                     ELCK"
     gene            complement(7853..8764)
                     /locus_tag="PLL59_06640"
     CDS             complement(7853..8764)
                     /locus_tag="PLL59_06640"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HMY23520.1"
                     /translation="MPILDFDKFKEDFNLFNHKKRVFTQPFFGTIIIAAGLFILYKLN
                     QYVDPWASWRQALGNATHFCEMNRFDQLIVQPSNTWSNLGYIIVGLIFLSIAKNDHKY
                     EHRKQVNNVLAKFPGFTYLIGFSVLYLGLGSFLYHATLTYFFQKLDQTGMYFILVSFI
                     AYVLFKLFPQIKFRGKIYVSNTFFMICAIIVQLLFFFFLWKLPINILFPALTISFFTI
                     NFLLIAKMRNSVSMVSLMKASLVSILIAASIWILDITDKLCSPTSIFQGHALWHILGA
                     LSIFLGYLYYRSETFLTPEEQEVTETI"
     gene            complement(8843..9604)
                     /locus_tag="PLL59_06645"
     CDS             complement(8843..9604)
                     /locus_tag="PLL59_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23521.1"
                     /translation="MNTKRILFYFISILLASSAIAQTNSSSKNKVVTTSKTNTTKSFA
                     FTGSYTMKFTVKETNGKMQSGEIKSAFEGVQMASIPTFSADKNMNVRTVFNMNDNTMT
                     MYFFDVKKNKKNGMMMKIPSLTQNNITTEQKITSTIQKTNETKIIDGYNCTKWIINYS
                     DGATCVAWISKDLHINTAEAISFFTKGMKGKKSPINTDCANINACALQSTYTAKDGST
                     VNMKMTDIKIGKPDAAFFSSSGYEVTDVTGIQFFK"
     gene            complement(9594..10424)
                     /locus_tag="PLL59_06650"
     CDS             complement(9594..10424)
                     /locus_tag="PLL59_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23522.1"
                     /translation="MKYLQRISIHDKDTFAKVVFAYYILLLLFRWHSSAFLTNIFAQP
                     LQSPGADNAFWLSHIIGFPQYIIQHYWASLLLDISLVVFAIACFFMATKKPVVCIVFI
                     IIYITHRITLESYACSHTKSMAAIFMAFLPFCCSKERNFYLTFEFSRYFLLYIMVASA
                     FFKVYNGMALQPNLFATTLINQHIDVAINQPTNFSYQIASILIKYPIIAGISYTALVI
                     VQAFFIIGFFTKKIDNILFILLLCFTISTYLLMRIYNFDIIILGLFLLFSSYNKKHEH
                     "
     gene            complement(10421..10969)
                     /locus_tag="PLL59_06655"
     CDS             complement(10421..10969)
                     /locus_tag="PLL59_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23523.1"
                     /translation="MSYSKWYIIRLFQQNKILFFFVCIFIFFQFYFNKKQIHSFPWYV
                     WDMYSRIETKPDTITQTAFYVDGKQLDITSIPIWEESTLLHTFNMYYSIQSNNYKDPI
                     ETSVKKRTKYLTLNMQNYIAHQIQNSEAAVRDYPRWLLRYVEKITHKNIKQVEVRKMY
                     YQYQNGKIKDLQRSELLLKINK"
     gene            complement(10969..11460)
                     /locus_tag="PLL59_06660"
     CDS             complement(10969..11460)
                     /locus_tag="PLL59_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HMY23524.1"
                     /translation="MEAAILISIGIILLVIGLVSCVLPPLPGPPIAYIALLIAAISLN
                     KTQDLPNWLLIVYAIITVFILIIDNFIPVWSTKKFGGTKAGIRGCFIGIVIGIIFSPF
                     GGISIILCPFLGAIIGEIVDGQSLSIAVKSGIGSFIGFLLTSGIKIILVLFMCYHYFK
                     AVF"
     gene            complement(11467..12081)
                     /locus_tag="PLL59_06665"
     CDS             complement(11467..12081)
                     /locus_tag="PLL59_06665"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HMY23525.1"
                     /translation="MSSSSKNILIALLIFIVSVLSIILLSKTLFTKKDNSTVDSTIVI
                     QKIQKVMKLVTVEGNYAELLRYNDFEYVDFPGFRKDAILKVKAKVSVGYNLENMKIST
                     DEKKKIIKIQQMPKPEIISIDTDISFENLSTGWFTSFSEAELTKLNNIAKQKIKEKAL
                     STELIRQAEEQKNDVFDLLFYMAKQNGYTIQVEGVTLQALSQSN"
     gene            12250..13029
                     /gene="surE"
                     /locus_tag="PLL59_06670"
     CDS             12250..13029
                     /gene="surE"
                     /locus_tag="PLL59_06670"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HMY23526.1"
                     /translation="MSRPVILVTNDDGIISPGIRALVEVMTKFGDVTVVAPDSPQSGM
                     GHAITIHDPLRLQKVDVFNDLQVPAYQCSGTPVDCVKIAIDKILRRKPDLCVSGINHG
                     SNASINVIYSGTMSAAMEAAIDGIPAIGFSLLDFAFDADFSAAQVYVERIVRNMMNTG
                     LTRDALLNVNFPKLPLAEIKGMKICRQANAKWEEEFDERRDPSGKNYYWLTGKFVNHD
                     QGEDTDAWALENGYVSIVPVQFDLTHHHAISFLNQNWNLQD"
     gene            complement(13057..13971)
                     /locus_tag="PLL59_06675"
     CDS             complement(13057..13971)
                     /locus_tag="PLL59_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HMY23527.1"
                     /translation="MNQLSDKEFKALITLLDDNDNEVFAHVSDKIISLGVEGIPLLES
                     AWETSENQIIQNRLEDLINRIQFSNVKDRLIKWINNGGVDLLEGALLIAKFQYADLDE
                     FKIKQKIESISKNIWIELNPALSPLEEAHVLNHVFFQLHGFFGLQTQQYDPELGYINN
                     LLDSKKGNSLSLSILYLILAQKNDLPIYGINLPYHFIMAYTRKHLKKEELDNNDAENM
                     VMFYINPLNKGIAFSRSEITHYLEQMKVKSNAKYYSPCNNLEIIKTLIYNQLSCYDQN
                     GNADKAHQLKELFDLFVNGNENLDIEEE"
     gene            complement(14042..16588)
                     /gene="topA"
                     /locus_tag="PLL59_06680"
     CDS             complement(14042..16588)
                     /gene="topA"
                     /locus_tag="PLL59_06680"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HMY23528.1"
                     /translation="MAKNLLIVESPAKAKTIEKILGKDFMVKSCYGHIRDLAKKEMGI
                     DVNNNFEPTYIISEDKEQVVKELQKLAKNAEVWLATDEDREGEAISWHLCEVLDLDKN
                     KTKRIVFNEITAPAIKNAVANPRKLDQNLFYAQQARRVLDRVVGFELSPVLWKKLGRR
                     TLSAGRVQSVAVRLVVEREREINNFNSASFYKVVAYFLSENKLFKAELSEKLKDEKEA
                     QQFLEEVKNALFKVADIQVKPTQKSPAAPFTTSTLQQEASRKLGFSVSRTMSVAQKLY
                     ESGKITYMRTDSPNLSDLALNSIQSYITKEYGKEYSNRKQYASKHSNAQEAHEAIRPT
                     YIENTNEGINRDEERLYDLIWKRTIASQMSNARLEKTVAKIDISTRKEQFIAEGEVLK
                     FEGFLKVYLEGTDDEDTEESGILPPLSVGQNLQYKNIEARQRFTQAPARFTEASLIKK
                     LEELGIGRPSTYAPTISTIQNREYVVKGEAEGKPREYQKLELNNKHEIKKTSHTESTG
                     SLKGKLIPTDIGLLVTDYLMENFSRVMDYSFTAEMEKDFDEIAEGNKQWSKILKQFYG
                     PFHKEIQASEDTAFVTGERELGIDKESGKTVVAKLGRYGPMIQIGNADDEEKPRYAKL
                     KSTQSIETISLDEAMELFKLPLVLGTYEGQEIAVNIGRFGPYVKFGEAFISLPKGEEP
                     TEVTLERAIEVIKSKQVEDAPIAHYQGKPITKGKGRFGPFIKWDGLYINIPVRYKFDT
                     INEVECADLIEKKIEKEANRFILNFPEEKISVENGRWGPFIKFNKKMLKLSGKKYTAE
                     EAALLTLEEIKSMIEAQVPNAFAKATAKKATSKKTTTTKASTKKTSTKKK"
     gene            16797..>17624
                     /locus_tag="PLL59_06685"
     CDS             16797..>17624
                     /locus_tag="PLL59_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HMY23529.1"
                     /translation="MKKNILMNALLSISPIDGRYRTKVENLENYFSEFALIKYRIQVE
                     IEYFIVLADELDEIGKLSEGQIVALRNLYQNFSIEHALRVKEIEKTTNHDVKAVEYFI
                     KENITDEQLLAKSEFIHFGLTSQDINNTAIPLSLKDFFSANYNNQFKVLLQELEQKVA
                     SYATISMLAKTHGQPASPTRLGKEFQVFVERLSWQYQYFAQIPYSAKFGGATGNFNAH
                     HVAYPAKDWKTFGNNFVNTTLGLSRSQTTTQIEHYDNLAALFHNYARINTILIDLCRD
                     "
BASE COUNT         5480 a         2903 c         2977 g         6264 t
ORIGIN      
        1 atagccaacg atacgaccac cacgctgcaa caaattatag ctttttctaa aattatcagc
       61 acctaagctg tcaaatgcaa catctatagg ctctttgatg acttgtgtaa aatcttcttt
      121 tgtgtaattt atgctcacat cagcaccttg ttctttacaa taggctaatt ttttttcact
      181 accagcagtg gtgtaaattt ttgcattctt tagtttgcat aattgaatga gcgcagtacc
      241 aacaccacca gcacctgcat gtatcaatac tttctcatgt ggaagtacat tcgtggccac
      301 tgaacaagca taaaaagcag tgctatattg agtcgccaat gccaatgctt tgcctgcgtc
      361 catgtcttct ccaatttttg ctatcgcatt ttctttttga atgacatatt gactataacc
      421 accaaatctg gtaaagacta aaacacgatc gcctttcttt acgcttgtaa cttttttccc
      481 tactttttca actcggccta ctgcttcata gccaacaata gtaggcagtg gcggacagtc
      541 acggtattgt cctaagcgtg ctgttacatc agcaaaatta atacctgatg cttccacgat
      601 gatgcctact tcgtcatcat tgggctcgct catattgata tttctaaatt caaaagcttg
      661 ttttgagggt ccatttttga ttaaaaatat tgcttgcatt tgattgtctt atttgattaa
      721 atgatactac ttaatttgta cgaaatttta tttgaaattc catttcgttt gtattgaatg
      781 tttatttttt tgcctattct tttgtttaat aagcgtaaag catcttccaa ttgtatgtct
      841 tcgcatttat aagtgtcaat ttttataatt tcatcgcctt gttgtaaacc agccatcaaa
      901 ggtggcgaat tttctgcgat gctcttgata ataaatccac catttttagt tttattttct
      961 agtaaaataa tattggaaat attgaattca aatttgtcat gtatatttct attgggcttg
     1021 aaatatacat atttctcagg ataggcgaag tagatgtcca ttctgttgag aatatcattg
     1081 ccaatatttc catctaaggt gtattcatca ggctctttaa aagtgatagg cgtttcatta
     1141 aaagcaataa taacgctatc aaaacgaagt gtgtcaataa ataatctttt tattctacca
     1201 acacttgaat acatagcgcc ggaaagtcct tctccaattt ctgcttgaat atattgattc
     1261 aaattcaaat cgctaggtac tttgttttta atgatgattg gaatgtttga gcctgtgtct
     1321 aacaataatt cagacataaa gatttcattg tttgaattca tgatagcgca gttgatatat
     1381 cctttgcttt ttctaatttg cacaggcagc ttagcaaaag tcgaattgat tttttttaca
     1441 ggctttttat ggtaaaagtc aatcattctt tttttataat tgataaaaat gtagtagcgc
     1501 tcaaatagtt ctgcaccaaa aattccatcg acatcagtgc ctaagtaatg gtctaaattt
     1561 aatgtgtctt tcgtaagaaa aaatattgga atattttctc cacgaattaa atcaatatct
     1621 attcgaccat tttccatttt tctagtttct atagaatctc taatgccaag acctgaaaaa
     1681 aatactttct gatgcaaagg tatttcaaat gagtctatcc atttaggatg aataacgatg
     1741 gtgcttttgc aaccagaatc gaaaataaat tttaatgttt ttgtttcatt tattttcacg
     1801 tcaacaataa ttatattatt gtataacttg aacggaattg atattttact tttgttgcta
     1861 ttaaaagtat aggttttagt attggcacgg acaaatgtga cattcatcat caaaaacaaa
     1921 accagcaaca agctttgaag taagcgcatg taataaaatt aaaataattt tgaatagtaa
     1981 gtgtattttt gcagcatgaa ttaccttacg gttaataatc tctgcaaata ttatggtgat
     2041 aagttgttat ttgaaaacat cacttttttt atcgataaag gtcaaaaagt agcattaatt
     2101 gcgaaaaatg gtactggaaa atcgtcattg ataaaaattt tacaaggctt ggagccacca
     2161 gaaagtggcg attataaatt tcataaagat atcagtgcgg gctttttggt gcaagagcct
     2221 gattttgacg ataacaaatc cgtctatcaa actataatgc atgctgataa tcctgtgttg
     2281 atggcgagtt tgaactatga atatgcatta gatggtcatg gtgatttgca aatggctatg
     2341 aataaaatgg atgaattgca agcttgggat tatgaacata aaatgaaatt gattttatct
     2401 aaatttctca ttttcgattt agaccagaaa gtaggcacaa tgtctggcgg tcaaaaaaaa
     2461 cgccttgcat tagcgatatt attgttggaa gagcctgata tatttatttt agatgagcca
     2521 accaatcatt tagatatgga tatgattgaa tggctcgaag attatcttac aaaaaacaat
     2581 atcacattat tgatgataac gcatgatcgt tatttcttag aaaatatttg tactgaaata
     2641 ttagagttag ataatggaat tttgtataaa cacaaaggaa attattccaa gtatttagag
     2701 aataaagaat tgcgggaaga aaatcaacga ataaacgtag aaaaggcaca aaataccttt
     2761 cgcaaagagt tagagtggat gcgtcgccaa ccaaaagcac gcaccacaaa ggcacaatcg
     2821 cgtgtagata attttgaaaa tgtaaaagcg gctgctactg cgaaagcaga agataagcgc
     2881 gtggaaatag aagtgaagat gcaacgcctt ggcggtaaaa ttttagagct acacaatatt
     2941 agtaaatcgc atggcgacaa aaaattattg cataagtttt ctcataaatt tcaacgaggc
     3001 gaacgcgtcg gcattgtcgg cagaaatggt tgtggcaaat caacattatt gaatataatt
     3061 acaggcacag aattacaaga tgatggcaat attgtgcttg gcgaaacagt agtgtttgct
     3121 tattacaatc aaaaaggatt gcaattgaaa gtagataaaa aagttataga cgtcatcaaa
     3181 gatattgcag aatatttgcc tattggaaaa ggtacgctca gtgcatctca attattagaa
     3241 cgcttccttt ttgtgccttc ccaacagcac aatcttgtgt caacgctgag tggtggcgag
     3301 cgcagacgct tgtatttatt gacaatttta atgaaaaatc caaatttttt aattcttgat
     3361 gaaccaacga atgatttaga tattttgaca ttgcaagtgc tcgaagattt tttattagac
     3421 tttaaaggtt gcttgttgat tgtaagccat gatcgttatt ttttagacaa actcacagaa
     3481 catctatttg tttttgaagg cgaaggtgtt gtgcgtgatt ttttaggaaa ttataatgag
     3541 tataggctga agctaaagga cgaacaacgt gaagcggcac gcgccgaagc acaacagcaa
     3601 acaaatacaa caaagccaac tgcaaattct aataagcgta aactgacttt taaagaacaa
     3661 acagaattta agcaacttga acaagatatt gacaccttga gcaatgaaaa atcagcattg
     3721 acagctcagt tgtctggcgg aatgcttcat catgacgaaa ttcaaaaaat tgcaaaacga
     3781 ttagaagaaa taattctata tttagatgct aaagaaaata gatggttgga gctgagtgag
     3841 tttgtgtaat attaatgcca tacaatatga caaaaaaata ttttcataat ttcaacggtc
     3901 ttcgttttta ttttgcatct tatattctct ttgcacacat cgaagagata aaatgggttt
     3961 tgaaattgcc taattttttt cacgatttta aatttgtcga accattaggc acatcagcga
     4021 ttacgctctt cttcactatg agtggttttt tgatttctta ttttctattc ttagaaaaag
     4081 ataaaaatga agacagtaaa atcaacttaa agaaatttta taaatcgcgt gtcgtacgca
     4141 tttggccttt gtattttttg tgtattgtgc tgtattggtt catcggtcct cattctgtat
     4201 tgaaagaatt atttaatagt atttacttca ctcaagtttt aaaaacacca tttactacag
     4261 atattccaca tgcagtatat ctcggattgt ttttgatcat attaccacaa cttgctggtg
     4321 caattgcatt tacttatggt ggtatggcaa tatatggtgg ccatgcttgg agtataggct
     4381 ctgaagaatt attttatctt ttttggcctt tattgattaa taaatttgtg agctacaaaa
     4441 agatattcaa agttggtttc attgctgtgt atagttgtta tggcttggtg attttagcat
     4501 tcatcgtaaa taaacttttt tttaaataca cgccattaaa cggtgttttg ctgacaataa
     4561 cttcctttct tgctttaaca agattgtatt gcatgctcat aggtggtttg ttagcctatt
     4621 tatttgtcaa taaccacaga tggcttagct tcttgcgcaa aaaatcggtg ctttatacag
     4681 cagtcgttat gttatttata atgattgcaa atggtttaga tgcaccgatg ctgatacatg
     4741 atatttatgc cttgttgtgg tctgttgttt tgctctattt tgccgacaac gaatctggac
     4801 ataagtggct aaacaataaa gttgtttctt atcttggttc tataacgtat ggtgtgtata
     4861 tgtatcatgt ttttgtcatc atcgtgttgt tgtattttgc acaacaatta aatatcacac
     4921 acgatttttg gctgtttaat atcttcttat acgtactgtc gccactgctc acatttacag
     4981 tgtcgcatta ttcctataaa tattttgaaa actggttttt ccgatttaaa taatgtataa
     5041 tattttttaa atgaacaatg acaatataaa aaaatacgct ttattgctga taagtatttt
     5101 ctcatttttc ttaggtgtac ttatcttttt tcaagtgtat atcgacgatg catttatttt
     5161 ttttaagtat ggatataatt tagttaatca tggcatttgg agttggagta ttgaaggcaa
     5221 gccagcggaa gcgtacacga gttttatata tgcagtatta tccatttttc cagcattgtt
     5281 gcatgtgccg ccacatattt ttattaaagt tgtaggctta ctttttttac tagcattgtt
     5341 gtttagagtg tatagtaaaa tcagcaataa aaaatgggct ttaatagcga tgcttgtcat
     5401 tactacaaat tggcaattat atacacatgc attttcaggt ttagaaacgg tgctttggtt
     5461 gtggctgctg ttggagtcat ttttccttat tgaaaaagag gaattgacgg taaaaaatca
     5521 aatgttgctt tggtcgattt gtttgctaat gacactcacg cgaccagaag gtgctatcta
     5581 tggttttttc ttttttgtat atcttgtttt ttataaaaaa caaaaattgc attttggcgt
     5641 attagcgacg cttattttta taggtattct ttattttgca gctcgttttg cctactttgg
     5701 attgccgttt ccattgcctt tttatcataa attaatggac agtcgatttg cgaaattagc
     5761 ttggattttt aatatctata catcttggta ttatatcatc tgtgccactt tagttttata
     5821 ttttttcaga aaacacaaag tgatatattt tattggcata attacttttt taattttcat
     5881 tggcttgtat ttacgtgcat ttttagtgat gaattttgca gatagatttc catttcaact
     5941 attctggcca ttcattttat ttgcacttat taagctggag aaatcgactt atgtagataa
     6001 agtaaaaata gtattgattg catgtttttt aaacatgatt attttttcaa aaggcttata
     6061 cgatgagaat ttgattgaat tagcatcgat tacgaataat gctggtgctg cttttttctt
     6121 gccacgttca cattatattt tggctaaaaa tatcaataaa attaaagatg tagaacgaaa
     6181 gaaattgaaa gtattatttg gcgatgcagg tatgtttcct tactatgtaa aagcacaatg
     6241 ctatgattat aatggcttgg ttgatgcaga gattgctatt aataaattag ataaaaaata
     6301 tttctatagt aaacaggcag acattgtttt gataggcaca cctaatgaaa ataaaatcgg
     6361 tttaaaacaa gatttatcta attgtaaaac tatttatgaa tatgccgaaa gcgactcaac
     6421 ttataaatat gttggcaaag taatcagtaa ggtaaatggc tattacgtgc atatttattt
     6481 taataccaaa tcaagctatg caaaagatgt agaaactgct ttaaataatg cagtcaccga
     6541 gtctgcaaat gcgcaattta gaataaagcg tttcttgaaa ttgaaatatt tagatttaga
     6601 aaacatctaa aatcaattta ttatttgcat aattctggct tgttgcaaat tccttttttt
     6661 gcaggaataa tctttttacc ttttatcgga tttccactaa tgatgccata agtaaaatca
     6721 ttcatgatgt ctattaattc gttatatcca attgatgaca tactgtgcga accattacaa
     6781 attacactta gagaaacatt gtcattattg agttttgcag cttcatagat agcagcgcca
     6841 ccatatacaa tcggaaatgt tttgcgctct ttacaacgaa acggattgcc agcatacata
     6901 tctatcaatt catcacaagt gccatgaatt agatgcatcg gtgtgtttat ttttttgcct
     6961 acaattacat tagggtcagc cactgcacca gctaaagcga ttacacctgc gactgtagca
     7021 tcgtaattca tattgtcgtt catagggtct aaagaaccac caacttgttc taatacattt
     7081 ttaggtgcat tttcagtggt ttgcacggcc aagccaatag tagcaagtgc tccagcgcta
     7141 tggccactca caaaaatctt atttttatcg atgccaaact tgtcagcatt tgcttttaaa
     7201 tatctaatag cagaacgcac gtcttgtacg ccacgcatat acgcaggata catgcctgtt
     7261 tggaaatctc caacacattg acccgccaac aatgcatttt tataaccaat tctatagtct
     7321 ggtaatgcga ctacatatcc tttttgtgca aatccttttc caatttcttg tgtgcaatca
     7381 tcaaactttc cagcaaaacc acctccaaat aaaaatatta tacagggtcg ttttttttcc
     7441 gcatcatttc tcggcgtaat cacatcgata atcaaatctt ttttagtgtt gtcccacatg
     7501 ggcgcatcgt gtttgtaata aaaagtatgg atatcaaaat cattaaaaat ataatcttta
     7561 tatcgaatgc cccaagggtt gggcgtagta actagcatgt cgccagaatt agcatatcca
     7621 tcaggcagta ttctgtcttt atttggtgga aacgctaatg tataagcacg cttaggcgct
     7681 gtgcctgcat tgagcgatga tgccgtttgt tgctgatgag atttttgtag attttgtaat
     7741 acagcatcac aacttgataa aataggcaat agcgtgataa gccacaccaa gcgcattttt
     7801 ttaaatgtca taaaaatgat ttgcctaaaa atacctaaaa attatgccaa atctatatag
     7861 tttctgtgac ttcttgctct tcaggtgtta agaatgtttc acttctgtag tataaataac
     7921 ctaagaaaat acttagtgca cctaggatat gccataaggc atgaccttga aaaatactag
     7981 taggcgagca aagtttgtcc gtaatatcta atatccaaat ggaagctgct attagtatag
     8041 aaactaatga tgctttcatt aagcttacca tcgaaacaga atttctcatt tttgcgatga
     8101 gcaaaaagtt gatagtgaaa aatgaaatag ttaatgctgg aaacaaaata ttgataggca
     8161 atttccaaag gaaaaagaaa aataaaagtt gtacgatgat agcacatatc atgaaaaaag
     8221 tatttgaaac ataaattttt cctctgaact ttatttgcgg aaataattta aataatacat
     8281 aagctatgaa tgaaaccaaa atgaaataca ttccggtttg atcgagtttt tggaaaaaat
     8341 aggtgagtgt tgcatggtat aaaaagctgc ctaatccaag atataacact gaaaatccaa
     8401 ttaaataagt aaagccagga aattttgcca atacattatt tacctgtttg cggtgttcat
     8461 atttatgatc atttttggcg atactcaaaa atattaaacc tacaatgatg taacctaaat
     8521 ttgaccatgt attacttggt tgtactatca gctggtcgaa tctgttcatc tcacaaaaat
     8581 gtgtagcatt gcctaatgct tgtcgccagc ttgcccaagg gtcaacatat tgattgagtt
     8641 tgtacaaaat aaataagcca gcggcaataa taatagtacc aaaaaatggt tgtgtaaata
     8701 cacgcttttt gtggttaaat aaattgaagt cttctttgaa tttgtcgaaa tctaaaatag
     8761 gcatcttgaa ttggaaattt ttattgaaaa taataaatct aatgctgtaa ttaccagata
     8821 caaatgttaa agaagtggta aattatttga aaaactggat gccagtcaca tctgttactt
     8881 cgtaaccact gctgctgaaa aatgctgcat ctggtttccc gatttttata tccgtcattt
     8941 tcatattgac agtgcttcca tcttttgcag tataggtaga ttgtaaagca caggcattga
     9001 tgttggcaca gtccgtattt attggcgatt tctttccttt catacctttt gtgaaaaagg
     9061 aaatggcttc tgctgtatta atatgtaaat ccttgctaat ccacgcaaca caagtagcac
     9121 cgtcgctata attaattatc catttagtac aattgtatcc gtcaataatt tttgtctcat
     9181 tggttttttg aatagtgctt gttatttttt gttctgtggt aatattgttt tgtgttaatg
     9241 atggaatttt catcatcatg ccattttttt tatttttctt gacatcaaaa aaatacatcg
     9301 tcattgtgtt gtcattcatg ttaaaaacgg tgcgtacgtt catgttttta tcagcagaaa
     9361 acgttggaat acttgccatt tgtacgcctt caaatgcaga ttttatttca ccactttgca
     9421 tttttccatt ggtttcttta actgtaaatt tcattgtgta gcttcctgta aatgcaaatg
     9481 actttgttgt atttgtcttc gatgttgtaa caactttatt tttcgaacta gaattagttt
     9541 gtgcaattgc cgatgaagcc agtaatatag aaatgaaata aaataagatt cgtttagtgt
     9601 tcatgctttt tattgtaaga tgaaaataat aaaaataatc ctaaaatgat gatgtcaaaa
     9661 ttataaattc tcattagtag ataagtggaa atggtaaaac aaagtaaaag tataaacaaa
     9721 atattgtcta ttttttttgt aaaaaatcca atgataaaaa atgcttgaac gattactaat
     9781 gcagtgtatg aaatgccagc aattattgga tatttgatga gaattgatgc tatttgatag
     9841 ctgaagttag ttggctggtt tatagcaaca tcgatgtgtt gattaatgag tgttgtggca
     9901 aataaatttg gttgtaatgc cattccatta tacactttga aaaatgcaga tgctaccata
     9961 atatatagca gaaaatatcg tgaaaactca aatgtcaagt agaaatttct ttctttgcta
    10021 caacaaaacg gtagaaatgc catgaaaata gctgccatac ttttggtgtg tgagcaggca
    10081 taactttcta atgttatgcg atgtgtgata tagattatta taaatacaat acaaacgaca
    10141 ggtttttttg ttgccataaa aaaacatgca attgcaaaaa caactaagct aatatcgagc
    10201 agtaaacttg cccaatagtg ttgaatgata tactgcggaa atccaatgat atggcttaac
    10261 caaaatgcat tgtctgctcc aggtgattgc aatggctgag cgaagatgtt agttaaaaaa
    10321 gcactgctgt gccatcggaa tagcaataac aaaatatagt atgcaaatac gacttttgca
    10381 aatgtatctt tatcatgtat tgatatacgt tgtaaatatt tcatttattt attttaagca
    10441 acaattcgct gcgctgcaag tcttttattt ttccattttg atactgataa tacatttttc
    10501 tcacttctac ttgtttgata tttttatgtg ttattttttc tacatatcgt aacaaccatc
    10561 ttggataatc ccttacagct gcttcgctgt tttgtatctg atgcgcaata taattttgca
    10621 tattcagtgt caaatatttt gttctttttt ttacgcttgt ttctattgga tctttataat
    10681 tattagattg tatgctgtag tacatattaa atgtatgcag caatgttgac tcttcccaaa
    10741 ttggaatact tgttatgtca agctgtttgc catcaacata aaatgctgtt tgcgttatgg
    10801 tatctggttt tgtttcaatt cttgaataca tgtcccaaac gtaccaagga aaagaatgga
    10861 tttgtttttt attgaaataa aactgaaaaa atataaaaat acagacgaaa aaaaacaaga
    10921 ttttattttg ttggaataat cgaatgatat accattttga atagctcatc agaatactgc
    10981 tttaaaataa tggtaacaca taaacaatac caatattatt tttatgccac tcgtcaataa
    11041 aaatccaata aatgagccaa tgcctgattt tactgctata ctgagcgatt gcccatccac
    11101 aatttctccg atgatggcgc caagaaatgg gcaaagtata attgatattc caccaaaagg
    11161 agagaagata ataccgataa cgatgccaat aaaacaacca cgaattcccg cttttgtgcc
    11221 accaaatttc ttagtactcc ataccggaat aaaattatct atgattaaaa taaatacagt
    11281 aatgattgca taaacaatca gcaaccaatt cggtaaatcc tgcgttttat ttaaacttat
    11341 tgcagcaatt agtagtgcaa tataggcaat tggtgggcca ggcaggggtg gcaacacaca
    11401 actcaccaat ccgatgacta agagtataat gcctatgctt attaatattg cagcctccaa
    11461 tgtgttttaa tttgattgtg ataatgcttg taaggtgaca ccttcgactt gtatagtata
    11521 accattttgt tttgccatat aaaatagcaa atcaaacaca tcattttttt gttcttcagc
    11581 ttgtcgaatc aattctgtag atagagcttt ttcttttatt ttttgcttag cgatgttgtt
    11641 gagcttagtt agttccgctt cactaaaact ggtaaaccag ccagtactta aattttcaaa
    11701 tgaaatgtct gtgtctatag atataatttc tggttttggc atctgttgta tcttaattat
    11761 tttctttttt tcatcagtac tgattttcat attttctaaa ttatagccga cagatacttt
    11821 agcctttact tttaatattg catcttttcg aaagccaggg aaatcgacgt attcaaagtc
    11881 attatagcgc aataactcag cataatttcc ttctacagtc accaatttca ttaccttttg
    11941 aattttttga ataacgattg tgctatcaac tgtagaatta tcttttttag taaaaagtgt
    12001 tttagaaagc aatataatac ttaatacaga aacaataaaa atcaataaag cgatcagtat
    12061 atttttcgaa gaagaagaca tactcaaaca taagtaaaaa tttgcaatct tgtatttttt
    12121 tggagctgtg ttgatgtggg cttgactttg agaataaaat ttattattac ataatgcatt
    12181 agccaagttt cgtgccctca cgtaccacat tgccatcaag caatgctcat ttttatgtat
    12241 atttgtgaca tgagcagacc agtaatctta gtaaccaatg acgatggcat catatcgcca
    12301 ggtattcgcg ccttagtaga agtaatgaca aaatttggcg atgtaacagt ggttgcacct
    12361 gatagtccac aatctggcat gggtcatgct atcaccatac atgatccttt gcgtttgcaa
    12421 aaagtagatg tgtttaacga tttgcaggtt ccggcttacc aatgttctgg aacgcctgta
    12481 gattgcgtca aaattgccat tgataaaatt ttgcgtagaa aaccagactt atgtgtatca
    12541 ggtattaacc acggttcaaa tgcgtcgata aatgttatct attcaggtac catgtcggca
    12601 gctatggaag cagcaattga tggcattcct gctattggtt tttctttgtt agattttgcc
    12661 ttcgatgctg acttctctgc tgcacaagtg tatgttgaac gtatcgtgcg caatatgatg
    12721 aacacgggat tgacgcgtga tgctttgcta aacgtcaatt ttccaaaatt gcctttggca
    12781 gaaattaaag gcatgaaaat atgcagacaa gccaatgcaa aatgggaaga agaatttgac
    12841 gaacgcagag atccaagtgg caaaaattat tactggctca ccggtaaatt tgtaaaccac
    12901 gatcaaggcg aagacacaga tgcatgggca ttagaaaatg gatatgtttc tatcgttccg
    12961 gtacagttcg atttaacaca tcatcatgca atttcttttt taaatcaaaa ttggaatttg
    13021 caagattaat ttgaatagaa tataattgta aattggctat tcttcttcta tatccaaatt
    13081 ttcatttcca ttgacaaaca aatcaaaaag ctcttttagt tggtgtgctt tatccgcatt
    13141 tccattttgg tcatagcaag acagctggtt ataaataagt gtttttatta tttccaaatt
    13201 attgcaaggt gaatagtatt tagcattcga ttttactttc atctgctcta agtaatgcgt
    13261 tatttcgctt ctcgaaaaag caattccttt atttagtgga ttgatataaa acatcaccat
    13321 attttcggcg tcattgttat ccagctcctc ttttttaaga tgcttgcgtg tataagccat
    13381 gatgaaatga taaggcaagt taatgccata aattggcaaa tcattttttt gagccaagat
    13441 gagatataag atacttaagc taagtgagtt tccctttttg gagtcgagca agttgttgat
    13501 ataacctaat tccggatcgt attgctgtgt ttgcaaacca aaaaatccgt gaagctgaaa
    13561 gaagacatga ttgagcacat gggcttcttc caaaggagat aatgcaggat ttaattctat
    13621 ccaaatgttt ttagaaatag actcaatttt ttgttttatt ttaaattcgt ctaaatcagc
    13681 atattgaaat ttggctatca ataaagcgcc ttccagcaaa tctacaccac cattatttat
    13741 ccattttatt agtctgtctt tgacattaga aaattgaatt ctattaatca agtcttctaa
    13801 tctgttttgg ataatttggt tttctgaagt ttcccatgcc gattctaaca gtggaattcc
    13861 ttctactcct agagaaataa ttttgtcgct tacatgtgca aacacttcgt tatcattgtc
    13921 gtctaacaaa gtaataagtg ctttaaattc tttatcacta agttggttca tacgtagttt
    13981 cttactacta atttataatt ttttaggaaa acaattttat agtttatcca cacaggtgag
    14041 cttacttctt ttttgtgctg gttttttttg tacttgcttt agtggtagta gtttttttag
    14101 aagttgcttt tttagcagta gcttttgcaa aggcattagg aacttgcgct tctatcatgg
    14161 attttatttc ttccaatgtc agcaatgctg cttcttccgc agtgtatttt ttaccgctta
    14221 gtttcaacat ttttttgttg aattttataa acgggcccca gcggccattt tctacagata
    14281 ttttttcttc agggaaattg agtataaaac gattggcttc tttttctatt tttttctcaa
    14341 tcaaatctgc acattctact tcattgatgg tgtcaaattt atagcgtact ggaatattga
    14401 tatacaagcc gtcccattta ataaatggac caaatctgcc tttccctttt gtaatcggct
    14461 ttccttgata atgtgcaatt ggtgcgtctt ctacttgctt agattttatt acttcgatgg
    14521 ctctttctaa tgtcacttct gttggttctt cgccttttgg aagtgaaata aatgcttcgc
    14581 caaattttac ataaggaccg aaacgaccaa tgtttacggc tatttcctga ccttcatacg
    14641 tgcctaatac caatggtaat ttaaacaatt ccatagcttc atcgagcgaa atggtttcaa
    14701 tgctttgcgt cgattttaat ttagcatagc gcggcttttc ttcatcatct gcattgccaa
    14761 tttgtatcat tgggccatat cttccaagtt ttgccactac agttttgcca ctttctttat
    14821 caatgcctaa ttcacgttcg ccagttacga atgcagtatc ttctgaagct tgtatttctt
    14881 tgtgaaatgg accataaaat tgcttaagta tttttgacca ttgcttgttg ccttctgcta
    14941 tttcatcaaa atctttttcc atttctgcgg taaacgaata atccattaca cgcgaaaaat
    15001 tttccattaa ataatcagtt actaataaac caatatcagt aggaatcagt ttgcctttta
    15061 aggaaccagt actttctgta tggctagttt tttttatttc gtgtttgttg tttaattcta
    15121 atttttgata ctcacgtggt ttgccttctg cttctccttt tacgacatat tctctatttt
    15181 gaattgtcga aatcgtaggc gcataggtcg atggtctgcc aatgcctaat tcttctaatt
    15241 ttttaattaa acttgcctct gtaaatcgtg caggtgcttg tgtaaatcgt tgtcgtgctt
    15301 caatattttt gtattgcaga ttttgaccaa cagacaatgg aggcaagatg ccactttctt
    15361 ccgtgtcttc atcatctgtg ccttctaaat atacttttaa aaagccttca aattttaata
    15421 cttcaccttc tgcaataaat tgttctttac gtgttgaaat atcaattttt gccactgttt
    15481 tttctagacg tgcattgctc atttgcgatg cgatagtgcg tttccaaatt aaatcatata
    15541 aacgctcttc atctctgttt ataccttcgt ttgtattttc aatataagta ggccttattg
    15601 cttcgtgcgc ttcttgtgca ttgctatgct ttgatgcgta ctgttttctg ttagaatatt
    15661 cttttccgta ttctttagtg atataagatt gtatgctgtt taatgccaaa tcagaaagat
    15721 taggtgagtc cgttctcata taagtaatct ttccactttc atatagcttt tgcgctacag
    15781 acattgtgcg tgaaacagaa aatcctaatt ttctagaggc ttcttgttgc agtgtcgaag
    15841 ttgtaaaagg tgctgccgga cttttttgcg ttggttttac ttgtatatct gcaaccttaa
    15901 aaagtgcatt ttttacctct tctaaaaact gttgtgcttc cttttcgtct ttcaatttct
    15961 ctgaaagctc tgccttgaaa agcttatttt ctgaaaggaa ataggcaact actttataga
    16021 aactcgcaga attaaagttg ttgatttcgc gctcgcgttc taccactaat cgcaccgcta
    16081 cagattgtac acggcctgcc gacaaggtgc gtctgcctaa ttttttccaa agtactggcg
    16141 acagctcaaa gccaactact ctgtctaaga cacgtcgagc ttgttgtgca tagaataaat
    16201 tttggtcgag tttgcgtgga ttggctactg cattttttat agcaggtgct gtaatttcat
    16261 taaaaacaat tcgctttgtt ttgtttttat ctaaatctaa aacttcacat aagtgccaag
    16321 aaatagcttc accttcgcgg tcctcatccg ttgctagcca tacttccgca ttttttgcta
    16381 atttttgtaa ctcttttacg acttgctcct tgtcttcaga tattatatag gtaggctcaa
    16441 agttgttgtt gacatcaatg cccatttctt tttttgctaa atcgcgaata tgaccataac
    16501 aagattttac cataaaatct ttccctaaga ttttttctat ggttttcgcc tttgcaggtg
    16561 actctacgat taataaattt ttcgccattt catatattta agtatcagca aagtaaaagt
    16621 agtgatatgc atttgttttt tactaaaaag tattactata tatatagtat aaacaatttt
    16681 tctgttccac tatttttact aaaaccgaat tttggcacaa ataaagctaa attttccatt
    16741 ttgttcacta tatcttattt aaaaatcaaa ttctttttgc ctttaattag ctatttttga
    16801 aaaaaaatat acttatgaat gccttgcttt ccatttctcc tattgatgga agataccgta
    16861 caaaagttga gaatttggaa aattatttct cagaatttgc tttaataaaa tatagaattc
    16921 aagtagagat agaatatttt atcgtattag ctgacgaatt agatgaaatt ggtaagttgt
    16981 cagaaggtca gattgttgcg ctaagaaact tgtatcaaaa ctttagcatt gaacatgctt
    17041 tacgtgtgaa agaaattgaa aaaacaacga atcatgatgt caaagcagta gaatatttta
    17101 tcaaagagaa tattacagat gaacagctgt tggcaaaaag tgagtttata cattttggtt
    17161 taacatcgca agacattaac aacacagcga ttcctttgtc cttgaaagat tttttttcag
    17221 caaattataa caatcaattt aaagtattac tacaagaact tgaacaaaaa gtagcatcgt
    17281 atgcgactat ttcgatgttg gcaaaaacgc atggacagcc agcatcgcca acacgcttag
    17341 gaaaagaatt tcaggtattc gtggaaagat tatcatggca atatcaatat tttgcacaaa
    17401 ttccctactc agcaaaattt ggtggtgcga caggaaattt taatgcacat catgtagcct
    17461 atccagcaaa agactggaaa acatttggta ataattttgt taatactact ttaggattat
    17521 cgcgctcaca aaccacaaca caaatcgaac attacgataa tttggcggca ctttttcata
    17581 attacgcgcg aatcaatact attttaatcg acttgtgtag agat
//
LOCUS       DAOQQV010000050        17536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1002_length_17536_cov_9.239918, whole genome shotgun sequence.
ACCESSION   DAOQQV010000050 DAOQQV010000000
VERSION     DAOQQV010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17536
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1002_length_17536_cov_9.239918"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..476)
                     /locus_tag="PLL59_06690"
     CDS             complement(159..476)
                     /locus_tag="PLL59_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23530.1"
                     /translation="MRHNKTIELPQKFFTILLLLNFLVFIIPINTGKCIAKQFTTSIP
                     AEEEHSHSHGLETSKILHQYKIYNFSKEIFSLFSKETIAHHQLQMLPVDWLETFSPPP
                     DIS"
     gene            920..2278
                     /locus_tag="PLL59_06695"
     CDS             920..2278
                     /locus_tag="PLL59_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HMY23531.1"
                     /translation="MKKSIYALKIVLSISIVVANIKQVLATHADSVVRIDTTLQTIVR
                     DTVYVETKKKKGKAYIAFKTWYNNQKEEGLSTNTNKTTKKWYEMIALRGYAQFRYNRL
                     AETNEKLKCEQCDRSWGEGGGFAFRRIRLVFYGQIGKHVYFYIQPDFASNASSSAQNF
                     LQLRDAYFDLGIGKHNEFRFRIGQSKIPFGFENMQSSQNRVTFDRSDAINSGIANERD
                     IGIFFYWAPEKMRTLFADVVRDGLKGSGDFGIIGLGIYNGQTSNKPELNKTPHVVLRA
                     CYPIKIKNQILEPAIQGYSGKYMLSKDALSSTVKFKSDRSYWDQRIAASLVLYPKPFG
                     IQAEYNFGRGPEFNKTNDSIETKKLHGGYITLSYFLPIKKHILYPYFRAQYYKGGKKH
                     ELDARSYEVQEYELGIEYQPIKQFEFVMAYVYSQRRFEDFVLQDNFQKGHLIRLQAQL
                     NF"
     gene            2308..2952
                     /locus_tag="PLL59_06700"
     CDS             2308..2952
                     /locus_tag="PLL59_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HMY23532.1"
                     /translation="MAISFLKFFQPKDKVFQNLFERGADVSVKISEVFLEAMKHTDDK
                     RFETLIQTGALEHQADDVVHNIFIELSKNFITPFDREDIHALAAAMDDVVDCIDEVGN
                     KMKNYEFTEFNEYILKMAELNNESVKELKIAIYELRNMKNLQKVNDACIKVHGYESKV
                     DLIYNQAMGDLIRNNKDNAVKIIVMKDLFEELEIISDKCQDVSNVIESIVIKYS"
     gene            3023..4690
                     /locus_tag="PLL59_06705"
     CDS             3023..4690
                     /locus_tag="PLL59_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PiT family inorganic phosphate transporter"
                     /protein_id="HMY23533.1"
                     /translation="MEFIIIVIAMALIFDYINGFHDAANSIATIVSTKVLTPFQAVLW
                     AAFFNFVAFLIFKDHAVANTIGKTVQENYITLPVIFSGLLAAIFWNLLTWWYGIPSSS
                     SHTLIGGFAGAAVAHAFMKNGVMPLQDIIDSGKVLKTIAFILFAPLIGMAISMFYTLV
                     MMNNNTWIKVGILSLTAVAIWFTFIHFQEKKIDDNIAKFFRIEKYEHALHDATSAEEK
                     EKLSKKLAQAQQDAQKSYTFISHYESRGSKVVANEIANTVDLDAVTAARIEDKLKVFF
                     EVKKLENKAKKDEYYKNASAKAKAAVDSLKPIAKLHASLGMNNMAEAMYDKGLVQPNK
                     HDEFVKMVQIDIKKDLAKEMDKSNNRIIRYGLIVMLAIIILSFIYNEKIKEPSAQRTA
                     NNFKKLQLLSSAAFSVGHGGNDAQKVMGIIAAALIANGNITDIKNMPDWVPLSCYLAI
                     GLGTLSGGWKIVKTMGSKITKVTPLEGVCAETSGATTLFLTEQMGIPVSTTHTITGAI
                     IGVGATKRLSAVRWGVTVNLLWAWILTIPVSALLASITYYIVQFFVK"
     gene            4811..5980
                     /locus_tag="PLL59_06710"
     CDS             4811..5980
                     /locus_tag="PLL59_06710"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMY23534.1"
                     /translation="MLSIFKINTNPEKRYFGFDIIRAFSVVLVMQMHFLQHFSQYLGI
                     NYFMLPLPDPVDMFFVCSGFLIGYQHLNEVHKLDKITPKYTARFLVMRWVRTLPSYYI
                     LLTFLVFLFALFSKKLDIPFRNYFFIQALYENRSKFYRETWTIAIEEWFYFTFPVLFY
                     IITSLLKVNKKKSFLIILLLYIFVCNFLKVYYYYHVFPSHTFDQWTKFREIVVLRIDT
                     IAIGLLAAYVCYFYTDFWDKYKYKFLPLGLIIYIGSIMFYWTWSNKIVTAEFATFYHY
                     TFYYLVSPISIMLCLPYLKTIRFKDNFVNHFILFTSLISYSIFLLHGTFLLLLFVLVY
                     MAVIKNVYIVYALWMFTTFFLANIFYNKIELPIMNMRRKIIEKLNLNENQSNNLR"
     gene            complement(5959..6402)
                     /locus_tag="PLL59_06715"
     CDS             complement(5959..6402)
                     /locus_tag="PLL59_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMY23535.1"
                     /translation="MVTQALCIIFERDLNKLKEEIRLYKSEDNIWKTANDVNNSAGNL
                     CLHLIGNLNHFIGAILGKTGYVRERNKEFSDKNIPIEKINNDIDATIIMVKNVLNNIS
                     DETLYSPFPEKKQGQEHPSIFILMHLLAHLEYHVGQINYHRRLLD"
     gene            complement(6458..7768)
                     /locus_tag="PLL59_06720"
     CDS             complement(6458..7768)
                     /locus_tag="PLL59_06720"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HMY23536.1"
                     /translation="MNRNYNILSNVLLLSFALFCFQQGFSIEKRKIKKQKIKNEITED
                     TARYKIHLDTVINGNNIEIHADTIFNKKYEFKKIIKNSETIADTTWQTKDVYSVNFKK
                     IDHIDYVPTDPAYAYTGKIKMSKPYSFFRPSEVLNKPRVALVSGMVAGLYAGANAWWS
                     AAWYSKYDRGKFHLFNDWGEWNQMDKIAHAYSCYFESKWHYDLYRWAGVKEKNAIWIG
                     MLMGNAWQLSIEINDGFQKKWGFSWGDMVMNVSGSLLFGIQQYLWHDQRINMKVSSFP
                     VNYGKYDDPEVKARAQKLYGTSFAEILLKDYNSMTIWWSVSPGAFIKNPNSKFPKCIQ
                     FSFGYGAGGMLGGYKNIWSKKDLGSDIDLGNTDPADIVDRTDIQRLRRFYFSFDIDWT
                     KLPVKKHWAKGLMKVLNIIKLPAPALEVNNNKNGSKVAWHWLKF"
     gene            complement(7883..8545)
                     /locus_tag="PLL59_06725"
     CDS             complement(7883..8545)
                     /locus_tag="PLL59_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23537.1"
                     /translation="MKKPTLLLAIMCITICISISSCKKDALEKQQQRIDEIIPAKYLD
                     TLKKLGATINTGIKPPNIEGIYYLNPAKLKASNIPGSVVGSIFGSVKIKFSSQNNDDF
                     SIQYISNQGSVSDTSQFTAISGSGNKFTVYGRTKATTSYNSDYAFFSIVFSGEKDGST
                     IKNLEWSFINSDDSHGGSTFIEEGQSRVIYDGDFNSESITTLRKNGTQPMQTNPSSIY
                     RR"
     gene            complement(8654..9322)
                     /locus_tag="PLL59_06730"
     CDS             complement(8654..9322)
                     /locus_tag="PLL59_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMY23538.1"
                     /translation="MSFRIAVTEDNAINRNTFMQKVQQMHDVKMVFIAENGNECLAEL
                     KGLPHNILPQVIFMDLEMPVMNGIETIQIAKALYPNIHFIVLTVFDDDDKIFEAIKAG
                     ASGYLLKHEPANILQEALINVIETGGAPMSPAIARKTLKLLSNTTNSISVNTENAFPN
                     FITEREKEILKHTINGWDAKRISMEINVSVLTVRKHIANIYEKLHVSSKAQIITLAHK
                     NKWI"
     gene            complement(9368..11263)
                     /locus_tag="PLL59_06735"
     CDS             complement(9368..11263)
                     /locus_tag="PLL59_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY23539.1"
                     /translation="MIDSLLHKINTSKNNNQKLTDLLLLFEQHHSIQKDTLLQYAMIA
                     QQLATQSTKVNKGLAQIAFLNAQLRFNITDTVLKIIAIELPKYNAAKQTERNIYFQLA
                     QLKSEAYNLQSDYVDAAEILYTKIKFAEKYKDTANIAISYNSLGEIAYNRNQLDESLN
                     WLKKAISITKKGEKYTAQNAVSYINLAGLYNWLDKIDSAKYYIDLSIPICKKIDNLYY
                     LCYAYLALSDYYKKNKKYKEAEYYMLEAMSLREKTEGKIIFSNEQLALGNLYYRSRQY
                     DKAIQVFKSGIAYDDSVKQFGKKKYNAKSNLEVKQYYYKGLAKCYKATNRAILYGEML
                     EKFIATNDTLNQLNAASAMAAMQVQYETQKKENTIIQQQLDLSKKNNLIYSIIGSIVA
                     LAIIFIILFWSYKSKQELKLQQQIEEDKWQTQQEVAKAEENERSRIAADLHDNLGVYA
                     ASIASNLSNIQIDNENKSNRIALNELRNNSQSIVTQLNDTIWVLQKNTILLTAISDRI
                     KLLTNRTQKSFPSIQFNITEDIEDDILFTASNAYQLYRIMQEAINNALKHSQCTQINI
                     KIVSNKKWTIEIHDNGIGIDTSVLNDSNTGNGLKNMTSRAIAANWNIKWKSDLKGTFV
                     IISPTIN"
     gene            complement(11424..12824)
                     /locus_tag="PLL59_06740"
     CDS             complement(11424..12824)
                     /locus_tag="PLL59_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23540.1"
                     /translation="MQIKFGTDGWRAIIAQEFTTDNVARVAKATALWVKSTSATPSIA
                     IGHDCRFAGPLFTEVSIKVLCNEGVKVYAHKGICATPMISLACVKLNTSAGVVITASH
                     NPPSYNGFKLKSNFGGPSTPAEIAKVEALIPEHADIPSTSLAAFEAQGLLEYVDLETM
                     YFNHVEASFDMNAIRNSGMIFGYDAMYGAGQKITRRLLPSNLVYLHCEENPGFNGQAP
                     EPLHKNLLEFSNLIKNSTDIEFGFANDGDADRIGCYDNEGNFIDSHHVILLLIHYLHK
                     YKGLTGKVAISFTVSDKIKIMCDAYGLPCEVTPVGFKYVCEIMINEDVLVGGEESGGI
                     AIKGHIPERDGVWMALVLMEFMAKTGKTIKELIAEVYAVVGTFDYDRYDLHISEEIKQ
                     KVIANCKNDEYKSFGKYTIERKEDIDGWKYFLPDGAWVMIRASGTEPVLRVYGQAKDR
                     AAVIELLDTVKAVLLS"
     gene            complement(12930..15362)
                     /locus_tag="PLL59_06745"
     CDS             complement(12930..15362)
                     /locus_tag="PLL59_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY23541.1"
                     /translation="MRNIIAYLFLLFGNTELIGKSISGIVQDEQKHAIEFANIILHKQ
                     DDKNYINGTTTDSSGQFEISGLDDGNYFMEIVFVGYKTDTVSAIRIEQETPVDIGIKT
                     LVIEDNLLKNVTISASRPVIERKADRIIYNVENSAKANGENIMDLLRTVPGVTVSGND
                     QIRINGKSDVQVMINGKVEQLNTEQLASLLKSIQSSNVKKIDVVSNPSARYDAESKGG
                     VLEIQLKTSQSTGVNGSVYANYRQNKYASTDVGFNINVNYKKLILSTNYNFGLQNNYQ
                     QNTYTRDFSNDSTKLRFAESSFAKSKFMSNYANLSLKYNINDKNTIGIGGEIFQFYDP
                     SSAFSNLNIIDNIYISENINAKQKTENSSYGKGINPSANFNFRSQLDSLGSTLELVYD
                     YTYSNLYTNSQLDMAYFDSIDNEKPIPHQIFKQDNPYIVNLHTARLNYNKPLKKGHEL
                     EFGVKFTWTKTTNDIQYKNFNGADFIYDSSKSNKFQYIENINAIFTTWSKSWKKGWST
                     NLGLRMEQTNTNQRSYTLNTLTKKHYFDFFPSLFIQKNIKDKHSININFSRKIKRPDF
                     NDLNPFQFYLSPYSIWTGNENLKPQLANVTEFTYTFDNAYSFFISHENLNNNYTHLIF
                     QDDSTKISTYRASNFKVRNNLNIGVNINKELFKWWTISYSAQYIFFRYNSIVNNSPFK
                     VTSHKFHISLDNTFILPKDFTINVFGFYTSPHYDATDIMLSDGMLNIAVSKSFFDKKL
                     RIRLAGNDILRTKNMSYTTNFFNVNSKTKNRWSSSFFSLSVSYNFQKGKKFQNSRVNK
                     SNEEEKGRIGGQ"
     gene            15605..16252
                     /locus_tag="PLL59_06750"
     CDS             15605..16252
                     /locus_tag="PLL59_06750"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HMY23542.1"
                     /translation="MQDTFRHQGLRKRLVQELRTLGINDEKVLSVIEKIPRHLFIETT
                     YGDDAYENKPFSIGRGQTISNPYTVAYQTALLNIKAGDKVLEIGTGSGYQSAVLAELN
                     AEIYTIERHQPLSKKAEKILKHFHYSNVHTYYGDGFKGLPEIAPFDKIIITAAAPEIP
                     KELLKQLAINGIMVIPFGDGDTQEMIRITRLASNEFEKETLSTCTFVPMLKGVVD"
     gene            16272..17528
                     /locus_tag="PLL59_06755"
     CDS             16272..17528
                     /locus_tag="PLL59_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23543.1"
                     /translation="MKKTTLLFWICCFMMQCAFAQSNNSKKQFDDLWNKTVNDQNGTT
                     KDLNALEKNKIESIDVTQKPVSNPNYNFKKVDTSNLEELSTKPIIKNASKGTAVKPKE
                     AVIDLKSKTKTSVPNKEKNVVTSPVKKVVNDTVKPKQKITTEDFSNKPIVKSKEKETS
                     SKPSMTPITTNTTKQKLDTAKGFREFTFETTPVVNNNATYHRKNEPLPKSKEQIEAEI
                     PVNRIATNKNTETENVQMKAAYAQYDKEADSLHSANKRRLDSIMQALNIKVPIVINPT
                     EFIDIFVNGGGVIANGDSKIYDRISILHTGNIQRETKSKNDGVQRSEKKISKDELTKL
                     AQYIVDMGFFDFNEDYDCTDEEAACNQRFSKSPQPVPLEVSLTVGQKKHKIKLAIYSP
                     KSDKNWVNYPANLEKIMNAIYTVVEK"
BASE COUNT         5700 a         2829 c         2904 g         6103 t
ORIGIN      
        1 agtgctatgt tttcaacata aaatactttc aattataaat acaaaaattg gatacagatg
       61 atgtatattg attccgtatg ctttgagaat atgcatacct aataatccat taagcgatta
      121 gcatagaata aactaaaatc aataaactag gtgaaagctt atgagatatc tggtggcgga
      181 gagaaggttt ccaaccaatc aacaggaagc atttgcaatt gatgatgtgc aatggtttct
      241 tttgaaaata aagaaaaaat ttctttcgaa aaattatata ttttgtattg gtgcaatatc
      301 ttgctcgtct ccaaaccatg actatggctg tgttcttcct ctgcaggaat gcttgtcgta
      361 aattgtttag caatacattt tcctgtattt ataggaataa taaatactaa aaaattcaat
      421 aacaataata tggtaaaaaa cttttgtggc aattcaattg ttttattgtg acgcaatttc
      481 tattatttta atcatatttt tattgctttc ttcattaaca tattcttatc taaaaaggtt
      541 caagtttgat gctttttttt cttcatgcct tttgtgaaaa atttttgtga atgttttgtg
      601 aacatttttc attaatcatg aatatttacc tacatgatta cacaggcttg tttttattag
      661 tttgataatc agggtattgt ttatttaaaa ttaattttac gtggataagt atttctttta
      721 ataataagtg aagtgcaaca actttaacca aaaatggtga actttgcact gatgatttta
      781 attaatcacg ataaatcaaa accagcattg cattttttga cacacactca aaatgctagc
      841 agtacaaatg ctattgacac attagcactt gtaaaaacac ttcataaagc aacacacaca
      901 cattattgta ttcagacaga tgaaaaaaag tatctatgca ttaaaaattg tattatctat
      961 aagtattgtt gtagctaata taaaacaagt tttggcaaca catgccgatt cagtagtgcg
     1021 aatagatact acactgcaaa ctattgtgcg agacactgtg tatgtcgaaa caaagaagaa
     1081 aaaaggcaag gcttacatag catttaaaac gtggtacaat aatcaaaaag aagaaggctt
     1141 atctaccaat accaataaaa ctacaaagaa atggtatgag atgattgcat tacgaggcta
     1201 tgctcaattt agatataata gactggcaga gacaaatgaa aaattaaaat gcgaacaatg
     1261 cgatagaagc tggggcgaag gtggcggctt tgcttttcgt cgaattcgat tggtgtttta
     1321 tggtcaaata ggcaagcatg tatattttta tattcaaccc gattttgcaa gcaatgcatc
     1381 gtcatcagca caaaactttt tgcaattgcg tgatgcttat tttgacttag gaattggaaa
     1441 acataatgag tttagatttc gcataggaca atcaaaaatt ccatttggat ttgaaaatat
     1501 gcaatcatca caaaatcgag taacgttcga taggagtgac gccatcaaca gtggtattgc
     1561 aaatgaaaga gatatcggca ttttctttta ttgggcacct gaaaagatgc gtaccttatt
     1621 tgctgatgtt gttcgcgatg gcttaaaagg atctggtgat tttggtatta taggtttagg
     1681 aatttataat ggacaaactt caaataaacc agagctgaat aaaacaccac acgtagtatt
     1741 gagagcttgc tatccaatta aaataaaaaa tcaaatttta gagccagcca ttcaaggata
     1801 ttctggaaaa tatatgctgt caaaagatgc actttcgagt accgtaaaat ttaaatcaga
     1861 tagaagttat tgggatcaac gcattgcagc ttctttagtg ttgtatccaa aaccatttgg
     1921 catacaagct gaatataatt tcggcagagg tccagagttt aataaaacaa acgactctat
     1981 cgaaaccaaa aaactacacg gtggctatat cacactgagt tattttttac ctattaaaaa
     2041 acatattctt tatccatatt tcagagcaca atattataaa ggtggcaaaa aacacgaact
     2101 ggatgctcgt agttacgaag tacaggaata tgaactcggt atagaatatc aacctataaa
     2161 acaatttgaa tttgtaatgg catacgtgta ttcgcaacgc agatttgaag acttcgtgtt
     2221 gcaagataat ttccaaaaag gacatttaat cagattacaa gcacaactaa atttttaaaa
     2281 ataattttaa taaataaatc aacaattatg gcaatttcat tcttaaaatt tttccagccc
     2341 aaagacaaag tatttcaaaa tttatttgaa cgaggcgcag atgtttccgt aaaaattagc
     2401 gaagtatttt tagaggcaat gaaacataca gatgacaaac gcttcgaaac attaatacaa
     2461 acaggtgctt tagaacatca agcagatgat gtagtacaca atatttttat cgaattgagt
     2521 aaaaatttta ttacaccatt cgacagagaa gatattcatg cgttagcagc tgccatggat
     2581 gatgtggttg actgtattga tgaagtaggg aataaaatga aaaactatga gttcacagaa
     2641 tttaacgagt acattttgaa aatggcagaa ttaaacaacg aatcagtaaa agaattaaaa
     2701 attgcaattt acgaattacg caatatgaaa aatttgcaaa aagtaaatga tgcctgtatc
     2761 aaagtgcatg gctatgaatc taaagtagat ttgatttata accaagcaat gggcgacttg
     2821 attcgcaaca ataaagacaa tgctgttaaa ataattgtga tgaaagattt gttcgaagaa
     2881 ttagaaataa tttccgataa atgccaagat gtttctaatg taattgaatc cattgttatc
     2941 aaatattcat aacaaacatt agtcttattc aaagcagaaa taaattagca gtatttcaca
     3001 agtgctcaac taaaaaacaa atatggaatt tataattata gtaatagcaa tggcactgat
     3061 atttgattat atcaatggtt ttcacgatgc cgcaaactcc attgccacta tcgtatctac
     3121 aaaggtgctt acaccatttc aagcagtgtt gtgggcagct ttttttaatt ttgttgcctt
     3181 cttaattttt aaagatcatg ctgttgccaa cacgattggc aaaacggtac aagaaaatta
     3241 tattactttg cctgttattt tttcaggatt gctcgccgca atcttttgga atctattgac
     3301 atggtggtac ggaattccgt catcttcttc tcacacactt atcggtggat ttgctggcgc
     3361 tgcagtagca cacgctttta tgaagaatgg tgttatgcca ttacaagata ttatcgatag
     3421 cggaaaagta ttgaaaacaa tagcttttat tctatttgct ccattaattg gtatggctat
     3481 ttccatgttt tataccttgg tgatgatgaa taataataca tggataaaag tgggaatatt
     3541 gtctttaaca gcagttgcaa tttggtttac atttatacat tttcaagaaa aaaagataga
     3601 tgataatatt gcaaagtttt ttagaataga aaaatatgaa catgcattac atgatgcaac
     3661 atcagcagaa gaaaaggaaa agctatctaa aaaactggca caagcgcaac aagatgcaca
     3721 gaaaagttac acatttatat cgcattacga aagcagagga agtaaagtgg tagcaaatga
     3781 aatagcgaat actgttgatt tagatgcagt aactgcggcg cgaatcgaag ataaactaaa
     3841 ggttttcttt gaagtgaaaa aactagaaaa taaagccaag aaagacgaat actataaaaa
     3901 tgcatctgct aaagcaaaag ctgctgtcga ttcattaaaa cctatagcca aacttcatgc
     3961 atctttgggt atgaataata tggcggaagc catgtatgac aaaggattag tgcaaccaaa
     4021 taaacatgat gagtttgtaa aaatggtaca aatcgacatc aaaaaagatt tagcaaaaga
     4081 aatggataaa tctaataata gaattattag atatggactc attgtaatgt tggcaattat
     4141 tattctttct ttcatctaca acgaaaaaat taaagaacct agtgcacaac gcacggcaaa
     4201 taattttaaa aaactacaat tgctatcttc agcagccttt agtgttggac atggtggcaa
     4261 tgatgcgcaa aaagtaatgg gcataattgc ggcagcatta attgcaaatg gcaatataac
     4321 cgatatcaaa aatatgcctg attgggtgcc tttgtcttgc tatcttgcaa ttggtttagg
     4381 cactttgagt ggtggttgga aaattgtaaa aacaatgggc tcgaaaatta caaaagtaac
     4441 accattagaa ggcgtctgtg cagaaacatc tggcgcaaca actttgtttt taactgaaca
     4501 aatgggcata cctgtctcaa cgactcacac gattactggt gccatcattg gtgttggtgc
     4561 tacaaaacga ttaagtgcgg tgcgctgggg cgttacggtt aatctattat gggcctggat
     4621 tttgacaatt ccagtgagtg ctttacttgc gagcattacc tattatattg tccagttttt
     4681 tgtaaaataa aataaatctt ttcaattgta aggcttcgga ttttccgaag ccttttttat
     4741 tgacttttta tgttgcgaaa aatttcaaaa tgtgtattac ttatggtatt ttagtcgatt
     4801 aaaaaaaaca ttgctatcta tatttaaaat caataccaat cctgagaaaa gatattttgg
     4861 atttgatatc atacgtgcct tctccgttgt gttggttatg caaatgcatt ttctacagca
     4921 ctttagccag tatttaggca ttaattattt tatgttgcca ctgcctgatc ctgtcgatat
     4981 gttttttgtt tgtagtggtt tcttaatcgg ttatcagcat ttaaacgaag tacacaaact
     5041 cgataaaatt acaccaaaat atacagcacg ctttttggtg atgcgttggg ttcgcacctt
     5101 gccgagttac tatattttac tcaccttttt agtgttttta tttgctctat tttccaagaa
     5161 attagatatt ccatttcgca attatttttt catacaagct ttatacgaaa accgttctaa
     5221 gttctatcgc gaaacatgga ctattgccat tgaagaatgg ttttacttta cgttcccagt
     5281 gctgttttat attattacgt cactactaaa agtaaacaag aaaaaatcat ttctgattat
     5341 tttgctcttg tatattttcg tttgtaattt cttaaaagtc tattattatt atcatgtttt
     5401 tcctagtcat acttttgacc aatggactaa atttagagaa atagttgtct tgcgaataga
     5461 cacgattgcc ataggtttgt tggctgctta tgtatgttat ttttacaccg acttttggga
     5521 caagtataaa tacaaatttc tgccattggg tttaatcatc tatattggaa gtataatgtt
     5581 ttattggaca tggtctaata aaattgtcac tgctgaattt gcaacattct atcattatac
     5641 tttttattat ttggtaagtc caatttcaat aatgctgtgt ttaccttatt tgaaaacgat
     5701 taggtttaaa gacaatttcg tgaatcattt tatattattc acgagtttaa tttcttatag
     5761 catcttcttg ctgcatggca cattcttatt gttactgttt gtattggttt atatggcagt
     5821 aatcaaaaat gtatatatag tgtatgcttt atggatgttc acgactttct ttttggcaaa
     5881 tattttctac aacaaaatag agttgccgat tatgaacatg cgaagaaaaa tcattgaaaa
     5941 gttaaacctg aatgagaatc agtctaataa tcttcggtga taattaattt gtcctacatg
     6001 gtattctaaa tgagccaaca agtgcattaa gatgaaaatg cttgggtgtt cttgtccttg
     6061 tttcttctct ggaaatggac tgtataatgt ttcgtctgaa atattattca acacattttt
     6121 taccattata atagtagcat cgatatcatt gttgattttt tctattggta tgtttttatc
     6181 gctaaattct ttattgcgct cacgcacata gcctgtcttt cctaatattg cgccaataaa
     6241 atgatttaag ttgcctatga gatgtaagca taaatttccg gcactattat tcacatcatt
     6301 tgccgttttc caaatgttgt cttctgattt atacaatcta atttcttctt ttaatttgtt
     6361 taagtctcgc tcaaagatga tacataatgc ttgtgtaacc ataagaaaat attttaaaat
     6421 tggaataatg aatacgattt tcagttgtta tgtttgctta gaatttcaac caatgccaag
     6481 ccactttact gccgttttta ttattattga cctctaaagc tggagcaggc aatttaataa
     6541 tatttaatac tttcatcaag ccttttgccc aatgtttttt tactggcaac tttgtccaat
     6601 caatatcaaa tgaaaaataa aatctacgta aacgctgtat gtctgttcgg tcaacaatat
     6661 cagccggatc tgtattgccc aagtctatat cacttcccaa gtcttttttg ctccatatat
     6721 ttttataacc accaagcata ccacctgcgc cataaccgaa tgagaattga atgcatttcg
     6781 gaaattttga attaggattt ttaataaatg cgccagggct aacactccac caaatcgtca
     6841 ttgagttata atctttcaat agaatttcgg cgaaggaagt gccgtataat ttttgtgctc
     6901 ttgctttcac ttccgggtcg tcatacttgc cataatttac aggaaatgat gaaaccttca
     6961 tgttgatacg ttggtcatgc cataaatatt gttgtatgcc aaacagcaaa ctaccactga
     7021 cattcatcac catgtcgccc caagaaaaac cccatttttt ctgaaatcca tcgttgattt
     7081 caatagataa ttgccaagca tttcccatga gcataccaat ccaaatggca tttttttctt
     7141 taacgccagc ccatctatat aaatcataat gccattttga ttcaaaataa caactataag
     7201 catgtgcaat cttatccatt tgattccatt cgccccaatc gttaaatagg tggaattttc
     7261 ctctgtcata ttttgaatac caagctgcgc tccaccatgc attagcgcca gcatacaagc
     7321 cagcgaccat gccagacacc aatgctacgc gaggcttatt gagtacttcc gatggtctaa
     7381 aaaaggaata cggttttgac atttttattt ttccagtata agcataggca ggatctgttg
     7441 gtacataatc aatgtggtct atttttttaa aattgacact atacacatct tttgtttgcc
     7501 atgttgtatc tgcaattgtt tcgctattct ttattatttt tttaaactcg tattttttgt
     7561 tgaagatagt gtctgcatgt atttcgatgt tattgccatt gataacagta tctagatgta
     7621 ttttgtagcg tgctgtgtct tccgtaattt cgtttttgat tttctgtttt ttgatttttc
     7681 ttttttcaat tgaaaatcct tgttgaaagc aaaacaacgc gaaagacaaa agcaatacat
     7741 tggacaagat gttgtaattt ctattcatag caacatcact aaaatacgca taaataatta
     7801 tttatactaa tcaacaaata gagcttgcct aattatatac tagacaagct ccgctttatg
     7861 aaaaaaaaat atggttgatg tcttatcttc tatatattga agatggatta gtttgcatag
     7921 gttgagttcc atttttccta agtgttgtaa ttgattcaga attaaagtct ccatcataaa
     7981 ttacacgaga ttgtccttct tctataaagg tagaaccacc atgtgaatca tctgaattaa
     8041 taaatgacca ttctaaattt ttaatagttg agccgtcttt ctcaccagaa aatactatcg
     8101 aaaaaaaagc gtaatcggaa ttatagcttg tagttgcttt tgttctaccg tacactgtaa
     8161 atttatttcc tgagcccgaa atagcagtga actgtgatgt gtctgataca ctaccttggt
     8221 ttgaaatata ttgaatacta aaatcatcat tattttgcga actgaatttt atttttacac
     8281 taccaaatat actaccaact acgctgcctg gtatgtttga tgcttttaat ttagctggat
     8341 ttaagtaata tatgccttct atattaggag gttttatacc ggtgttaatg gtagcaccta
     8401 atttctttaa tgtatctaaa tattttgcag gaatgatttc atcgatacgt tgctgctgtt
     8461 tttctaaagc atcttttttg caagatgaaa ttgatatgca tatggtaata cacatgatag
     8521 cgaggagaag agttggcttt ttcataagtt tattattttt ggatacataa agatgtgata
     8581 agccacgaaa gttatcaata cttaatttgt agtaggctag attgtgagct aatctccact
     8641 ttcgaaattt atcctatatc catttgtttt tatgtgctaa tgtaatgatt tgcgcttttg
     8701 aactcacgtg tagtttttca taaatattag ctatgtgctt tctcacagtc agcacgctaa
     8761 catttatttc catgcttata cgcttagcgt cccagccatt tatagtatgt ttcaaaattt
     8821 ctttttctct ttctgttata aaattaggga aagcattttc cgtatttaca gaaatagaat
     8881 ttgtagtatt gcttaataat ttcaatgttt tgcgtgcaat cgcaggactc attggcgcgc
     8941 cacctgtttc aattacatta attaaagctt cttgtaaaat attcgcaggc tcatgtttta
     9001 ataagtagcc agatgcacca gctttaatag cttcgaatat tttatcatcg tcgtcaaaaa
     9061 ccgttaatac aataaagtga atatttggat acagcgcttt tgcgatttgt atcgtttcaa
     9121 tgccattcat cacaggcatt tctaaatcca taaatatgac ttgtggtaaa atgttgtgtg
     9181 gtaagccttt taattctgct aagcactcat ttccattttc tgcaataaat accattttca
     9241 catcatgcat ttgttgtact ttttgcatga atgtgttcct attaatggca ttatcttcag
     9301 tgacagcaat acggaagctc atcgtaattt ttttaaattt atcaatcttt tctaaaatgg
     9361 taaataatta atttatagta ggagatatga taacaaaagt accctttaaa tctgatttcc
     9421 atttaatgtt ccaattggca gctattgctc gcgatgtcat atttttcaag ccatttcctg
     9481 tatttgaatc gttaagcact gaggtatcta tgccgatgcc attatcatga atttcaattg
     9541 tccatttttt attgctaact atttttatat ttatttgtgt acattgactg tgttttaaag
     9601 cgttgttaat ggcttcctgc atgatgcgat aaagttgata agcatttgaa gcagtaaata
     9661 atatatcatc ttcaatatct tctgtaatat tgaattgaat agaaggaaaa cttttttgcg
     9721 ttctattagt taataatttt attctatcac taattgctgt aagtaaaata gtgttttttt
     9781 gtaaaaccca aatcgtgtca ttgagttgag taactataga ttgtgaatta tttcgtaatt
     9841 catttaatgc aattctattg cttttatttt cattatcaat ttgaatgttg gataaatttg
     9901 atgcgatgga agctgcataa actcctaaat tatcgtgcaa gtcagcggca attctactgc
     9961 gttcattttc ttctgctttg gcaacttctt gttgtgtttg ccatttatct tcttcaattt
    10021 gttgttgtaa ttttaattct tgtttacttt tataactcca aaataaaatt ataaaaataa
    10081 ttgcaagtgc aactatgcta ccgatgatgc tatagataag attatttttt ttagataaat
    10141 ccaactgctg ttgaatgatc gtattttctt ttttttgtgt ttcgtattgt acttgcattg
    10201 ctgccatcgc actggccgca tttagctgat ttagggtgtc gttcgttgca ataaattttt
    10261 cgagcatctc gccatataaa attgctctgt ttgttgcttt atagcatttt gccaagcctt
    10321 tgtaataata ttgcttcact tccaagttag attttgcatt gtattttttt ttgccaaatt
    10381 gcttgacaga atcatcgtaa gcaattccac ttttaaacac ttgaattgct ttgtcatact
    10441 gtcttgatct gtaatacaaa ttgcctaatg caagttgctc gtttgaaaat attattttgc
    10501 cttccgtttt ttcacgaagc gacattgctt ccaacatata atattccgct tctttgtatt
    10561 ttttgttttt tttatagtaa tcagataatg ccaaatatgc gtagcataaa taatataaat
    10621 tatctatttt cttacatatc ggaattgata aatcgatata atattttgca gaatctattt
    10681 tgtctaacca attgtataat ccagccaaat taatatagct aactgcattt tgtgcagtat
    10741 atttttctcc ttttttagta attgaaatgg ctttttttag ccaatttaaa ctttcgtcga
    10801 gttgatttct attataagca atttctccta aagaattata tgatatagca atgtttgctg
    10861 tgtctttgta cttttctgca aactttattt tagtatataa tatttcagcc gcatctacat
    10921 aatcagattg taagttataa gcttcacttt taagttgagc caactgaaaa tatatatttc
    10981 tttcagtctg tttggctgca ttatattttg gcaactcaat ggctataatt tttaatacgg
    11041 tgtctgtaat attaaatcta agctgcgcat ttaaaaatgc aatttgagct aaacctttat
    11101 ttacttttgt gctttgtgtt gctaattgtt gagcaatcat tgcgtattgt aataaagtat
    11161 ctttttgtat gctatgatgt tgctcgaata aaagaagtaa atctgttagt ttttggttgt
    11221 tattttttga agtatttatt ttatgtaata aactgtctat cactcgagtc tgcgagtgca
    11281 gaacactact aaaagcaata aaaatacata gcagtatgtg ttttcttatc ttcatatagc
    11341 agctgcctat aataaagata tcgaaataaa atgaaaaatg ccgaatataa tattcggcat
    11401 ttttaagaga gtaaacgtct gtgttatgat aaaaggacag cctttaccgt gtctaataat
    11461 tcgataactg ctgctctgtc ctttgcttgt ccatacacac gcaacactgg ttctgtccct
    11521 gaggcacgta tcatcaccca agcaccatct ggcaagaaat atttccaccc atcgatatct
    11581 tctttgcgct cgattgtgta ttttccaaaa cttttgtatt catcattttt gcaattagca
    11641 atgacttttt gttttatttc ttcagagatg tgtaagtcat atctgtcata atcgaaagtg
    11701 ccaaccactg cataaacttc agcgatgagt tctttaatag ttttaccagt tttggccatg
    11761 aattccatta aaactaatgc catccacacg ccatcgcgtt caggaatgtg tcctttaatt
    11821 gcaatgccac cactttcttc tccaccaact aatacatctt cattgatcat gatttcgcat
    11881 acatatttaa aaccaacagg agtaacttcg catggcaatc cataagcgtc gcacatgatt
    11941 tttattttat cggaaacagt aaatgaaatg gctacttttc ctgttaatcc tttgtattta
    12001 tgcagataat gaataagcaa taagatgacg tgatgcgagt cgataaagtt gccttcgttg
    12061 tcataacaac caattctgtc tgcatcgcca tcgttggcaa atccaaattc aatatcagtt
    12121 gagtttttga tgagattaga aaactctaat aaatttttat gtaatggctc tggtgcttgt
    12181 ccattaaatc ctggattttc ttcgcaatgt aaatacacta aattgctcgg caataatcga
    12241 cgtgtaattt tttggccagc accatacatt gcgtcatagc caaatatcat accgctgttt
    12301 ctgattgcgt tcatgtcaaa tgaagcttct acatgattga aatacatagt ttctaaatca
    12361 acatattcta ataatccttg tgcttcaaaa gccgccaatg acgtactcgg aatatctgca
    12421 tgttctggaa ttaatgcttc tacttttgca atttctgcag gcgtgcttgg tccgccaaaa
    12481 tttgatttta acttaaaacc attatagctc ggaggattgt gtgatgccgt aataacaacg
    12541 ccagcactgg tgtttaattt tacgcaagct aatgatatca ttggtgttgc gcagatgcct
    12601 ttgtgcgcat acactttcac gccttcgtta cacaatactt ttatacttac ttcagtgaaa
    12661 agtggtccag cgaaacggca gtcgtgaccg atagcaatgc ttggtgttgc agaggtggat
    12721 tttacccaaa gtgcagttgc ttttgctaca cgtgcaacat tatctgttgt aaattcttgc
    12781 gcaatgatgg ctctccagcc gtcagttcca aattttattt gcatattgaa aaattaattt
    12841 ccaaaagtac aaaaagaata gaatattact gaaagtaaaa tgtcaaatgc atagattgaa
    12901 ttttttatgt tttgtcaatt ggaaatatat tattgtccac caattctacc tttttcttcc
    12961 tcattactct tattgacgcg tgaattttgg aactttttac ctttttggaa attataggat
    13021 acgcttaagc taaaaaaact cgaactccat ctgttttttg tcttggaatt gacattaaaa
    13081 aaattggtag tgtaggacat attttttgtg cgtaaaatat cattaccagc caaacgaatt
    13141 ctcaattttt tatcgaagaa tgatttagaa acagcaatat ttaacatgcc atcgctgagc
    13201 ataatatctg tagcgtcata atgtggtgaa gtgtaaaatc caaatacatt aatcgtgaaa
    13261 tcttttggca atataaatgt gttgtccaat gaaatatgga atttgtgcga ggtaacttta
    13321 aaaggtgaat tatttactat tgaattgtac ctaaaaaata tgtattgtgc tgagtaagaa
    13381 attgtccacc atttaaataa ttctttatta atatttacgc caatatttaa gttgttgcgc
    13441 actttaaagt tggaagcacg ataagtagaa atttttgtac tatcatcttg aaatattaag
    13501 tgtgtataat tgttgttcaa attctcatga ctaataaaaa aactataagc attgtcgaat
    13561 gtgtaagtaa actcagtaac attggcaagt tgtggtttta gattttcatt gccagtccaa
    13621 atagaatatg gacttaaata gaattgaaaa ggattcaaat cattaaaatc agggcgttta
    13681 atttttctac taaagttaat attgatgctg tgtttgtctt tgatattttt ttgaatgaac
    13741 aagctcggaa agaaatcaaa gtaatgtttt tttgtcaatg tatttaatgt ataggaacgt
    13801 tgattcgtat tggtttgttc catgcgcaat cctaagttag tactccagcc ttttttccag
    13861 cttttgctcc atgttgtaaa gatggcattg atattttcaa tgtattgaaa cttattagat
    13921 ttactgctgt cgtagataaa atcagcgccg ttaaaatttt tatattggat atcgttcgtt
    13981 gtttttgtcc aagtaaattt cacaccgaat tcgagctcat gtcctttttt taatggcttg
    14041 ttgtaattca atcttgccgt atgtaaattg acaatataag gattgtcctg cttgaaaatt
    14101 tgatgaggaa taggtttctc attgtcaata gaatcaaagt atgccatatc aagctgacta
    14161 ttcgtatata ggttagaata ggtgtaatca tacaccaatt ccaatgtgct accgaggcta
    14221 tctaattggc ttctaaaatt aaaattagcg gaaggattaa tgcctttgcc ataagaactg
    14281 ttttctgttt tttgtttagc attgatattt tcggaaatat aaatattgtc gataatattc
    14341 aagtttgaaa atgcggaact tgggtcataa aattgaaaaa tttcaccacc aatgccaata
    14401 gtatttttat cgttgatatt atatttgagg cttagattgg cataattgct catgaattta
    14461 ctttttgcaa aactagattc agcaaatcga agtttggtgc tgtcattgct aaagtcgcgc
    14521 gtgtatgtat tttgctgata attgttttgt aaaccaaaat tgtaattcgt gctgagtatt
    14581 aattttttat aatttacatt gatgttaaag cctacatctg tgctggcata tttattctga
    14641 cgataattag catatacgga gccattcacg ccagtgcttt gagatgtctt caattgaatt
    14701 tctaaaacgc caccttttga ttctgcatca tatcgcgctg atggattgct gaccacgtct
    14761 atttttttta cattgctact ttgaatcgat tttaataagc tggcgagttg ttctgtgttt
    14821 aattgctcta cttttccatt aatcattact tgcacatcgc tttttccatt gatgcgtatt
    14881 tggtcgttgc cactcacggt tacgccaggc acagtgcgaa gtaaatccat aatattttcg
    14941 ccatttgcct ttgcgctatt ttctacgtta taaatgatgc gatctgcttt gcgctcaatt
    15001 actggtcgag atgcagatat ggtaacattt tttagtaaat tatcttcgat aacgagtgtc
    15061 ttaatgccaa tatctacagg cgtttcctgc tctatgcgaa tagctgaaac cgtgtctgtt
    15121 ttataaccga cgaatacaat ttccataaaa taattgccat catctaaacc actaatttca
    15181 aactgaccac tgctgtctgt ggttgttcca ttaatgtagt ttttgtcatc ttgcttatgt
    15241 aaaatgatat tagcaaattc gattgcgtgc ttttgctcgt cttgaacaat accagaaatg
    15301 ctctttccta ttagttctgt gttcccaaat agtaagaaca gataagcaat aatatttctc
    15361 atagcttttt ttgtgtgtta ttggtataac acttttaaaa aatattacac aatctgctaa
    15421 atattttaaa atatgatacc aaatcactgt tttcaaagtt atgatgcgaa ttgcggtact
    15481 atagttaatt tttttaagct tgcttaaatt aattagtatt taaagtgtac tatttgtctt
    15541 atttattaac attattgaat acattttcct ttattaaggc aacaaagaag tttaattttg
    15601 cctcatgcaa gatacgttca gacatcaagg attacgtaaa agattagtac aagaattgag
    15661 aacattaggc attaatgatg aaaaagtgct tagtgtaatt gaaaaaattc caagacatct
    15721 ttttatagaa actacttatg gcgatgatgc ctatgaaaat aaaccatttt caattggtcg
    15781 tggtcaaaca atttcaaacc cttatactgt tgcttatcaa acagcattac taaacataaa
    15841 agcaggagat aaggtactag aaattggcac tggctcaggt tatcaatcag cagtattagc
    15901 agagctgaat gctgaaatat ataccatcga gcgccatcag ccattatcaa aaaaggcaga
    15961 aaaaatttta aaacattttc attattcaaa tgttcatacc tattatggtg atggttttaa
    16021 aggattgcca gaaattgcac cttttgataa aattatcatt acagcagcag ctcctgaaat
    16081 tccgaaagaa ctgctaaaac agttggcaat aaatggcata atggtcatac cttttggtga
    16141 tggtgatacg caagaaatga tacgcatcac tcgattagct agcaacgaat ttgaaaaaga
    16201 aacactttct acatgtacct ttgtgccaat gctaaaaggt gttgttgatt aatatattaa
    16261 attatttaga gatgaaaaaa actacacttt tattttggat atgttgcttt atgatgcaat
    16321 gcgcattcgc acaatcgaac aattcgaaaa aacaatttga cgatttgtgg aacaagacag
    16381 taaatgacca aaatggtacc actaaagact taaatgctct cgaaaaaaat aaaatcgaat
    16441 cgatagatgt aacacaaaaa ccagtaagca atccaaatta taatttcaaa aaagttgata
    16501 ccagtaattt ggaagaactt tcaacaaaac ctattataaa aaatgcttca aaaggtactg
    16561 ccgtaaagcc taaagaggct gtcattgatt tgaaatcaaa aacgaaaacg agtgtgccga
    16621 acaaagaaaa aaatgtagta acatctccag taaaaaaagt agtaaatgat acggtaaaac
    16681 caaagcaaaa aattactacg gaagattttt ctaacaaacc tattgttaaa agcaaagaaa
    16741 aagaaacgtc gagcaagcct tcaatgacgc ctattacaac aaacacaaca aaacaaaaat
    16801 tagatactgc taaaggattt agagagttta cgtttgaaac gacaccagtc gtcaataata
    16861 atgcaacata tcatagaaaa aatgagcctt tgccgaaatc gaaagagcaa atagaagcgg
    16921 aaattccagt gaatagaatt gctacgaata aaaatacaga aactgaaaat gtgcaaatga
    16981 aagcagctta tgcacaatat gataaagaag cggattcctt acactctgcc aacaaaagga
    17041 gacttgatag tattatgcag gctttgaata ttaaagttcc tattgttatc aatccaacag
    17101 agtttattga tatttttgta aatggtggcg gtgttatcgc taatggcgat tcaaaaatat
    17161 atgatagaat ttctattttg cataccggaa atattcaacg agagacaaaa tctaaaaacg
    17221 atggtgtcca acgaagcgag aaaaaaatat cgaaagatga attgacaaaa ctggcgcaat
    17281 acatagtaga tatgggcttt tttgatttca atgaagacta tgattgtaca gatgaagaag
    17341 ctgcgtgtaa ccaacgtttt tctaagtcgc cacagccagt gccactcgaa gtgtctttga
    17401 cagtaggaca gaaaaaacat aaaataaaat tggccattta ttcaccaaaa tctgataaaa
    17461 attgggtgaa ctatccagca aatttagaga aaataatgaa cgccatttat accgtagtag
    17521 agaagtaata aataga
//
LOCUS       DAOQQV010000051        17320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1015_length_17320_cov_8.144454, whole genome shotgun sequence.
ACCESSION   DAOQQV010000051 DAOQQV010000000
VERSION     DAOQQV010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17320
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1015_length_17320_cov_8.144454"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="PLL59_06760"
     CDS             <1..1793
                     /locus_tag="PLL59_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMY23544.1"
                     /translation="EYYGADNIYATDVQLELSAAYLQNHQIKEATMAANKALSITQAG
                     NKILYPYDNWLCNVQLLECKKAMYKQQAPSLLQIKNDIAIINHAIAVASAIRHTLYTS
                     GSQTYYTEKMAILNELGIYFITKWYKKKDAFFYQQLFYFAENDKANLLRSKIINYEND
                     KILPLAEKKKANLISGKLNYFYTLNEDIASNTSINDSILKYNKQFEQFTHYIEKKYPK
                     IHYLKYGNKNISIEKIQSEIQEDHSFLAYFKDSEDYYCMSISNANVHFMHCGKVEYID
                     SLVHLFQTAILSKEYDKETAALLSKSILPIGIKKQLIVSPAGELSKVAFDVLQADNRY
                     LIYTHSTQFAFSASTYFLSRNAIFNKNVLGIFPDFTTSNLAVLNTQKEEKAIQLFDEY
                     DLLKDSLITKDNIIKKTQTLGLVHFGTHITIDTVQPLRSTLLLQTKDNYNLCINDIWK
                     LNLNTQLVTLAACQSNFGKQQVGEGIKNFAWAFHYAGARNILSTQWNASNKATATIIS
                     DFYSNIRHGESKANAIQNAKLNYLKNTDNIGAQPFFWSNFNLYGDESEIALETPFLAN
                     FWWLPLLLSAMLYFGLTRFRRYYKSRRYGY"
     gene            1870..3000
                     /locus_tag="PLL59_06765"
     CDS             1870..3000
                     /locus_tag="PLL59_06765"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23545.1"
                     /translation="MLLPLEYHFPDNTVSAKRLDRLLASGYFRTGNYMLRTRVLYFND
                     EILNTLHIRIQLKEHTFSKSLQKILQQNNKRFTYNILPFNITVEKEGLYKAHSKRFKG
                     NASPTLSSYMLDTSNKSVFNTYEINIYDNGKLIAFSFFDIGINSMASIIGIFDQNYTK
                     HSLGIYTMLLEIEYAKSIQLKYYYPGYVAYEPSQFNYKLRLSDRFDFYDWYSKRWTSF
                     ENRHKKQKVNDFLKQKMQLAKTWLQNFKLPFQELFYPYFYMGSMYPKSDCVKSVHHLQ
                     IQDFDIEGLFYIVEFQPEKMELSLSGVTIHKYQFEEYIDFSETNDGKKWNRVLLYLQP
                     SIIIRNEYELYAGYIFLQELFRQHLLPPPLNKATSQESTTEE"
     gene            3039..3635
                     /locus_tag="PLL59_06770"
     CDS             3039..3635
                     /locus_tag="PLL59_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY23546.1"
                     /translation="MDITELLPTAQRVMKHYDITFLTPKEWAMLIQYGTLRSFKKGEN
                     FITANQINTKIAFILKGGFRFFYEKNDNEITCLLSFENGLIGSFESNILKQPCTQTIQ
                     AIEESELFIIDYRDLEALYDKSTKFERVGRMILEYYIAFLQQRITSYLLDTPEERYRT
                     LVNKTPDLLNRVPLQYIASYIGVTPVSLSRIRKRILKK"
     gene            3831..4079
                     /locus_tag="PLL59_06775"
     CDS             3831..4079
                     /locus_tag="PLL59_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23547.1"
                     /translation="MSTVSSTIKEKLHQYIDSAEERKLHAIYIMLEDEIEYEEDYTDE
                     FKNELDKRFAEYKLDGVAIEAAAAKERIDSLMQKLKSR"
     gene            4079..4369
                     /locus_tag="PLL59_06780"
     CDS             4079..4369
                     /locus_tag="PLL59_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMY23548.1"
                     /translation="MYSVAYKPKALEEYEQSIEWYAERSIKAAENFILDVQESIKEIK
                     ANPTQFKNRYKKYYETTLKKYPFDIVYIIEAERIVIISIYHQKRNPKKRYRK"
     gene            complement(4651..6483)
                     /locus_tag="PLL59_06785"
     CDS             complement(4651..6483)
                     /locus_tag="PLL59_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HMY23549.1"
                     /translation="MNIVPNKLSLAQLFANSNEQFIVPSYQRRYSWKIQHTGALFDDI
                     DLLKDNDGHLFGMIILHAGAYNGGFMTPELVDGQQRLTTLTILLKSIEKAYRKIEREE
                     TANEIRKMLQCKGPDKVLKNKILLGDLDNPDYQIITVDGDFDEIKNKHLKDAIECFDF
                     WLSEYEEEDLDLFYHKLINVAVIIRLDVSLAQDAYKLFETINNRGLKLSATDIIKNFL
                     LGHASKINDDNTLEQVKLIWAQIITELDGINTDNFFRQYFCSILKRKITFNGLVEEFK
                     TYYIKNVENTDLLGEFEVYSDTSIDDEEDSVFDNGDENNTEENYQELEEQPSMKVTNI
                     EFLKRIRSASEVYRKMCYEDFAEQWMNIHITNLWRVQCFPSFIFLMHFFQKDYTKKTK
                     INVLKMIETFMLRRHICERRTGENDYIFSQLMNCLEEKTEDEMLYSFRERLMDYYPED
                     AEFEDKLPTYQFKGKVEDRARYILEQLEYDKRGNTKETLISTSEDVHLEHIVPQTINT
                     KKAISEFGDWITYLGEKALVKHKKYVSRIGNLTLLAAPLNIQASNNPFTRKKGSYRQS
                     DLHITKDLATKSDFKFYHIDQRGEEIAKKAIEIWSIDFSDIEDE"
     gene            complement(6538..7716)
                     /locus_tag="PLL59_06790"
     CDS             complement(6538..7716)
                     /locus_tag="PLL59_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HMY23550.1"
                     /translation="MKLQFPEGFIWGTSTAAYQIETASENNWKGLKCHDGTIFDKCSQ
                     HDLHRDEDLEYICQLGNAYRMSHDWAKLQKAPFAPFDPKEVEPYLRFMEELKRRGKHI
                     MLVLHHFTNPLWFEKEGSWEKEGNRKMWLDFVQKSVDTFGHLVDTWNTFNEPNVYISN
                     GWILGSFPPFKKGKILTARKVLKEIGRAHDEAYDIIKAKSTAPIGISHNTVKFVGEIF
                     PGHLIAKVFDWWFMDYCHKHFLKVDFQGLSYYARMSFRPMPVDNIFSPGKLEKLGRRV
                     DLMWEYEPKGFYEMFHRYWNKTKKPIIMTESGVCTHDPKFRIESIKEYLGWVHQAQKE
                     GIPIQGYFHWSTMDNHEWNIGQYYRFGLVHVDFETGKRTMTEAGTFLGEVAKNNSVEV
                     "
     gene            7822..8403
                     /locus_tag="PLL59_06795"
     CDS             7822..8403
                     /locus_tag="PLL59_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23551.1"
                     /translation="MQSNSLEYNNNRIQVRNRKSMLEIRIPIKKNGIALLMMLLATAA
                     WTVGLYVLVRIELHLQYFWYKAGLILAIAAWFVLGMTVASFFIWLFFGRERILVTNKY
                     LITDKPLVLFYRRNIYDIAAIFNLRTDIEIYKANRGGTWIDESRTVIRFDTTDKLVTF
                     ARGITKQEAEFILLQLAKSTYLQKQQFAVEQHF"
     gene            complement(8400..10922)
                     /locus_tag="PLL59_06800"
     CDS             complement(8400..10922)
                     /locus_tag="PLL59_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMY23552.1"
                     /translation="MRKFGLLACMLLVFSVLYANCPRKKSKKKYHSQLEEIVVTPQNF
                     ITSYNASYQKLFDLQRTKLHLTPNFKQKTLSGKATLTLKPHFYEQNQLVLDAKWMQIA
                     AVALKTNETYKTLKFTYDNLLLKIDLDKYYSKNDQIEIEIDYVALPYQQDSLQVEDGR
                     GLYFIDTEDKNPYKPMHLWSQGESESASCWFPTLDATNQKSTQEIFVTIDTAMVSLSN
                     GLLIDSKPNGDGTKTDHWKQDKPHSPYLFFLGVGDYYISRDKWRDKEVTAYTFPKYKD
                     DVAEIFKNLPAMMEFFSNILGVDYPWDKLGNIMSYDYTAGAMENTSAIIYFDRMLCHR
                     QQLIDGDFDWIIAHELFHHWFGDLVTAESWANLTINESFADYSEYLWYDFRDGKDEGD
                     AVKYKSTQKYFRTYKYKNEPIVNYYYVHPSDVFDGIRYEKGGAVLHMLRYYVGDDAFF
                     KSLNKFLQQYKFNNAELSDLRKCFEETTGEDLNWFFNQWWLDKGHPILDITHQFDEKN
                     KTIALTVRQTQRSIDGPTFRIPTKVDIYSNNTKETKVIDITDRIQTFYFPSDTVPQLV
                     NFDADKVLVCEKTENLSTAQNLFKYYNAPLFVDKLEAINALALVQKANVTIQELLLKA
                     LQDKNWTLREEALDAIEIDKFDNKTKLSLALQQVIYTDNNSHVREKAIQKYARLEKNK
                     SIDVLETVLKKDSSFICLSAALAQLNTYDKSKAYTYATKLSSTENSSLLVAIGRIFKD
                     TTADNMEFFKKAIWLNGPYTFYSNFRSFADYLEKANNTDLEKGILFLKDIYTYEESNY
                     NKQGAKQIVGNLYYYFNEKAKKDSQASVKLQIVKKAGKELLN"
     gene            11093..11596
                     /locus_tag="PLL59_06805"
     CDS             11093..11596
                     /locus_tag="PLL59_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HMY23553.1"
                     /translation="MRYKVFYKEDNAHEIELDEQQIKIDGKATDIDLVKLLDNKFHIL
                     QNHKSYNIEVLHTDYNLKRFSIKVNNNIYDLNLQTELDILLEKMGMSAAGDEKMDNVK
                     APMPGLVLDVLVEIGQAVKKGDNLLILEAMKMENIIKASGNGIVKSIKINKKDAVEKG
                     QLLIEME"
     gene            11681..12316
                     /locus_tag="PLL59_06810"
     CDS             11681..12316
                     /locus_tag="PLL59_06810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HMY23554.1"
                     /translation="MHIVPHDIEKYIDAHTSAESAVLQQLNRKTQTDVLMPQMLSGKV
                     QGQFLKFISQMVQPSRVLEIGTFTGYAAICLADGLSADGKLFTIDINEELEDIVRTHV
                     EKAGLQNKIVQIIGNAAQEISKLNETFDIVFIDADKQNYSLYYDLVIDKVRNGGFILA
                     DNVLWSGKIIDEKKDKDTQKLAEFNDKVQQDNRVENVIISIRDGIMMIRKK"
     gene            12319..13848
                     /locus_tag="PLL59_06815"
     CDS             12319..13848
                     /locus_tag="PLL59_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HMY23555.1"
                     /translation="MKIKYSILFFTYLLLSTVSINAQKLDTASIIAYIKLYKDIAVSE
                     MQRTGIPASITLAQGIHESGFGKSYLAQNTNNHFGIKCKEEWKGKTFKYTDDAPNECF
                     RVYDKVEDSYIDHSNFLKYRPRYADLFLLDKYDYKGWSYGLKKAGYATNPKYPVILIK
                     TIEDFELTAFDKGINPAYLQTTLESINKPIANDEQLKKDAPEIIQHTFEVEKKTVIES
                     VNKQNTVPVSTKKLNKTITKINKCKTVKIYPSKGETLDLIGNVMRIDKEKLLIFNDVN
                     DDYVWKDGQTIFIQQKKKSNKTEPSYKVKQEDNLWSISQKKGVRLSSLLKMNKLSANE
                     EPAVKETIMLKGKSKLKPALRSQKNTDSTIKNTIVSTPTKNVIYDTIIRARDTVYPNI
                     QTDAKLSVDSNKILGWESETKLLEKPSAITYPVQSKPESIVTVEKTNTTTANMPTVYP
                     KTIDYAKLPKSSTGLHTVIKGDTMYNICKRYNITTAQLMEWNNLSDQSVKLGQELKIQ
                     Q"
     gene            13873..14412
                     /gene="hpt"
                     /locus_tag="PLL59_06820"
     CDS             13873..14412
                     /gene="hpt"
                     /locus_tag="PLL59_06820"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HMY23556.1"
                     /translation="MQTIQIKDKLFKQYISDAEIQHRIQEIVAELSSKYHDKSPVFIV
                     VLRGAFMFASDILKKYNHPCEIEFVKLTSYTGMESTGKINVSLPIKEEVIKDRHVIII
                     EDIVDSGLTMDFFSHYIQGMQPASVALVSFLYKPENLVKPVNIDIVGFTIPELFVIGY
                     GLDYDGEGRNLSAVYQVAD"
     gene            complement(14409..15080)
                     /locus_tag="PLL59_06825"
     CDS             complement(14409..15080)
                     /locus_tag="PLL59_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HMY23557.1"
                     /translation="MIKKIFFLHALLLCILMNSIAQKSAAQWLALAKKGEKNLVNADL
                     AGADLSKLDLHGVNLSGANLSGANLSSSKLYDCNLSNANLSGANLSKADLFAAKMQYA
                     NLQKANLTQALLQEAVLQFADLTEANLQTANLLLTDLKGSNLQGANVTNAIFDYAQTS
                     SITAQVNSAPEMVETEALGNALTLLERNINVFVRMSGAKINKNTKGVNFVWAKKNGAV
                     IVAAN"
     gene            15358..15891
                     /locus_tag="PLL59_06830"
     CDS             15358..15891
                     /locus_tag="PLL59_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HMY23558.1"
                     /translation="MSNSLNEYQIPHAGLKKDIHEFSYELTEKFFANFENALIQKCEI
                     QVNIILDNRQEPLLLEIDIDGIVWSDCDKCTAPIPIVIHSTFEINVKYTNDETLKHTD
                     ETDIMYIARDEQYIDIAQYLYDFVHLSIPVHVICDQPGKTDYCDMEIIGLLEKQQTTS
                     DTDPRWSGLDKLKDKLN"
     gene            15898..16086
                     /gene="rpmF"
                     /locus_tag="PLL59_06835"
     CDS             15898..16086
                     /gene="rpmF"
                     /locus_tag="PLL59_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HMY23559.1"
                     /translation="MAHPKRRQSTTRRDKRRTHYKMEAPNVVTCKVTGESHLSHRAYV
                     SGNDLYYNGKVLIEGYVK"
     gene            16164..16727
                     /gene="efp"
                     /locus_tag="PLL59_06840"
     CDS             16164..16727
                     /gene="efp"
                     /locus_tag="PLL59_06840"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HMY23560.1"
                     /translation="MASTSDIRNGMCINFNSDIYKVVEFLHVKPGKGAAFVRTKLKSI
                     TTGRVLENTFPAGAKLDEVRVEYREYQYLYPENDAYTFMNNETYEQITVSGNIINAPE
                     FLEEGMNVKISVRAEDEMPLTCELPATVIVEVTYAEPGIKGDTATNTLRPSTVSSGAK
                     VMIPLFINVGDKIKVNTETGAYMERVK"
     gene            16862..17320
                     /gene="accB"
                     /locus_tag="PLL59_06845"
     CDS             16862..17320
                     /gene="accB"
                     /locus_tag="PLL59_06845"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HMY23561.1"
                     /translation="MDIAQIKKLIQLLNDNHLGELKVQDGDFKISIKHRDYAKTSGVA
                     SVISAPVATSTPSIAQETATAPKKEETPSGHYQTVKAPMIGTFYRAAGEGKEPFVKVG
                     DKVKKGDVLCIIEAMKLFNEIQSEVSGKIVKVMVDNAQAVEYDQALFLIE"
BASE COUNT         5987 a         2935 c         2886 g         5512 t
ORIGIN      
        1 aagaatacta tggtgctgat aatatttatg ccaccgatgt acaattagaa ttatcagctg
       61 catatttaca aaaccatcaa ataaaagaag ccacaatggc tgcaaataaa gcattatcca
      121 tcacacaggc tggaaataaa atattgtatc cgtatgacaa ttggttgtgt aacgttcagc
      181 tgctggaatg caaaaaagcg atgtacaaac aacaagctcc aagtttatta caaataaaaa
      241 atgatatcgc aatcatcaat catgctattg ctgttgcaag tgcaatacgg cacactttat
      301 acactagcgg cagtcaaacc tactacacag aaaaaatggc catactaaat gaacttggca
      361 tttattttat aacgaaatgg tacaaaaaga aagacgcatt tttttatcaa cagctattct
      421 attttgccga aaacgacaaa gcgaatttat tgcgtagcaa aattatcaac tatgaaaatg
      481 acaagatttt gcctttggca gaaaagaaaa aagctaattt aatttctgga aagctcaatt
      541 atttctacac cttgaacgaa gatattgcat caaacacatc tatcaatgat tccattttaa
      601 aatacaataa acaatttgaa caattcacac attacatcga aaagaaatat ccaaagatac
      661 attatctaaa atatggaaat aaaaatatta gcattgagaa aattcaatca gaaatacaag
      721 aagaccatag cttcttagca tattttaaag atagcgaaga ctattattgc atgtctatta
      781 gcaatgcaaa cgtacatttt atgcattgcg gaaaagtgga atacattgac tcattagtgc
      841 atttatttca aactgctatt ctatctaaag aatatgataa agaaacagct gctttgcttt
      901 caaaaagtat tttgcctatt ggcataaaaa aacaattgat tgtctcgcca gctggcgagc
      961 tatcaaaagt ggcatttgat gttttgcagg ccgataacag atatctgatt tacacacaca
     1021 gcacacaatt tgcattttct gctagcacct atttccttag cagaaatgct attttcaata
     1081 aaaatgtact tggtatattt cctgatttca ctactagtaa tttagcggta ttaaacacac
     1141 aaaaagaaga aaaagctatc caattatttg atgagtatga cctcttaaaa gacagcttaa
     1201 ttacaaaaga taatattatc aaaaaaacac aaacgcttgg actcgttcat tttggcacac
     1261 acatcacgat tgacactgtg caaccattac gttctacgct tttgttacaa accaaggata
     1321 attacaatct ttgtataaat gatatttgga aattaaacct caacacgcag ttagtaacat
     1381 tggctgcatg tcagagtaat tttggaaagc aacaagttgg ggaaggcatt aaaaattttg
     1441 catgggcttt tcattatgct ggagcgagaa atattttatc gacgcaatgg aatgcatcaa
     1501 acaaggctac tgctacaatc attagcgatt tttattcaaa catcagacat ggcgaatcta
     1561 aagccaatgc gatacaaaat gcgaagttaa attatttgaa aaacactgat aacattggcg
     1621 cgcagccgtt tttctggtca aattttaatt tatatggcga cgaatcagag atcgcattag
     1681 aaactccttt tcttgcaaat ttttggtggt taccactatt actttcagca atgctttatt
     1741 tcgggctaac tcgttttaga cgttattata aaagcagacg ttatggatat taatgttgaa
     1801 tagaatagca aggttttaaa aaaagtatta caaaatttta ttaattttag cttcaaccct
     1861 tgcgcaacta tgctactacc tttggaatat cattttcctg acaatacggt ctcggcaaaa
     1921 cgattagata gattgttggc atcaggttat ttcagaactg gaaattacat gttgcgtact
     1981 cgtgttttgt attttaatga tgaaatatta aatacgctac acattcgaat tcaattaaag
     2041 gaacatacat tttccaaaag tttgcaaaaa atactacagc aaaacaacaa gcgatttact
     2101 tataacattc tacctttcaa tattacagta gagaaagaag gcttatacaa ggcacatagc
     2161 aagcgattta aaggcaacgc ttcacctact ttgtcgagct atatgttaga cacaagcaac
     2221 aagagcgttt tcaatacgta cgaaataaat atttatgaca atggcaagct cattgctttt
     2281 agtttctttg atattggcat aaatagcatg gcaagcatca ttggtatatt tgaccaaaac
     2341 tatacaaaac atagccttgg catatataca atgttgctgg aaatagaata tgcaaaatct
     2401 attcaattaa aatattacta tccaggttat gtagcttacg agccatctca atttaattat
     2461 aaacttcgct tatccgaccg atttgacttc tatgattggt attccaagcg atggacatct
     2521 tttgaaaaca ggcataagaa acagaaagta aacgattttt taaaacagaa aatgcaactc
     2581 gcaaagacat ggttacagaa tttcaagctg ccttttcaag aattatttta tccctacttt
     2641 tatatgggca gcatgtatcc aaaatctgat tgtgtgaaaa gtgtgcacca tctacaaatt
     2701 caagattttg atatcgaagg cttgttctat atcgttgagt ttcaaccaga aaaaatggaa
     2761 ttatcacttt caggtgtgac cattcacaaa tatcaatttg aggaatacat cgatttttca
     2821 gagacaaatg acggaaaaaa atggaatcgt gtgttattat atttgcaacc aagcatcatt
     2881 attcgcaatg aatatgagtt atatgcaggt tatatttttt tacaagaatt gtttaggcaa
     2941 catctattgc ctccaccatt aaataaagca acatcgcaag agtcaacaac agaggagtaa
     3001 gcatttaatt acttaatttc gtatctttat ataacattat ggatattaca gaactattgc
     3061 cgaccgcaca gcgtgtaatg aaacattacg acattacctt tttaacacca aaggaatggg
     3121 caatgctgat tcaatatggt acgctacgct cctttaaaaa aggagaaaac tttatcaccg
     3181 ccaaccaaat aaatacaaaa atcgctttta ttctaaaagg tggttttcga tttttttatg
     3241 agaaaaatga taatgaaata acgtgcttat tatcgtttga aaatggacta atcggcagtt
     3301 ttgaatctaa tattttgaag caaccttgca cacaaacgat acaagctatt gaagaatcag
     3361 aattgtttat tatcgactat cgcgatttag aagcattata tgacaaaagc actaagtttg
     3421 aacgtgttgg ccgcatgatt ttagaatatt atatcgcatt tttacagcaa cgtattacct
     3481 cttatttatt agacacgcct gaggagcgct atcgaacttt ggtaaacaaa acacccgatt
     3541 tattgaatcg cgtgccttta caatatattg catcttatat tggcgtaacg cctgtatcat
     3601 taagtcgaat acgcaagcga attttgaaga aataaaaata tcatttgtag tattagtttt
     3661 cacatacttt ttaggcacct attttccgat gttattggct tattggtgac gcatgtaatg
     3721 tacatagata tgctatctaa acacctgcaa agcaaggcga ctttcatcca attagttaat
     3781 ttaacacaac gaaactttaa aaaagctaaa tttgataaaa ataaaataag atgagtacag
     3841 taagttccac catcaaagaa aagttacatc aatacattga ttctgcggaa gaacgcaaat
     3901 tacatgctat ctatattatg cttgaagatg aaatagaata tgaagaagat tatacagatg
     3961 aatttaaaaa tgaattggat aagcgatttg ccgagtataa gttagacgga gttgcgatag
     4021 aagcagcagc agccaaagaa cgaatagata gtttaatgca aaaattaaaa tctagataat
     4081 gtatagtgtt gcgtataaac caaaagcatt agaagaatac gaacagagca tagaatggta
     4141 cgcagaaaga agtattaaag cagcagaaaa ctttatctta gatgtacagg aaagtattaa
     4201 agaaataaaa gccaatccaa cacaatttaa aaataggtac aaaaagtatt acgaaaccac
     4261 attaaaaaaa tatccattcg atatagtgta cattattgag gcagaaagaa tagtcatcat
     4321 atctatttat catcagaaac gaaatccaaa gaagagatat aggaaataaa catgattcat
     4381 acagttgcaa cgcactagcc atctttagcc tttggtgttt agttttcaga gctatagcgg
     4441 agcaaatttc acccttagct aaaaaaactc aaatacatct aactaaattt ttataaaaca
     4501 acgatttggc gcttggcgtt cgttcgggat ttcagggcat aaaactgtca acccaacact
     4561 aaagctgatt tgaaaaaccg cacataattt taccaccgtc acctgcatca cgccaatcgc
     4621 atgttatagc cagaggtttt gtaattcgtc ttattcatct tcaatatctg aaaagtcaat
     4681 gctccaaatt tcaattgctt tttttgcaat ttcttcacct ctttggtcaa tgtgataaaa
     4741 tttaaaatcg gattttgtcg ctaagtcttt tgttatatgt aagtcagatt gtctatagga
     4801 tccttttttt ctcgtaaatg ggttgtttga cgcctgtatg tttaaaggtg ctgctaatag
     4861 tgtcaagttc ccaattctgc tcacatactt tttatgtttt actagtgctt tttctcctaa
     4921 atatgtaatc cagtcaccaa actcactaat tgctttttta gtatttattg tctggggaac
     4981 aatatgctcc agatgaacat cttctgatgt gctaattaaa gtctctttgg tattacctcg
     5041 cttatcatat tctaactgtt ctaaaatata tcttgctcta tcctcaactt tacctttaaa
     5101 ctgatatgtt ggtagcttat cctcaaattc tgcatcttca ggataataat ccattaatct
     5161 ttccctaaat gaatacaaca tttcgtcttc tgttttttct tcgagacaat tcattagttg
     5221 cgaaaaaatg taatcatttt cacctgttct tcgttcacaa atgtgtctgc gtaacatgaa
     5281 cgtctcaatc attttgagaa cattaatttt agttttcttc gtgtagtctt tttgaaaaaa
     5341 gtgcattaag aaaatgaaag aaggaaaaca ttgaactctc cagagattag taatatgaat
     5401 attcatccat tgttctgcaa aatcttcata gcacattttt cggtagactt cacttgcact
     5461 tcttatccgt ttaagaaact caatatttgt aaccttcata gaaggttgtt cctctagttc
     5521 ctgataattt tcttctgtgt tgttctcatc gccattatca aaaacggaat cttcttcatc
     5581 atcgattgaa gtgtccgaat acacttcaaa ttcacctaac aaatctgtat tttctacatt
     5641 cttgatgtag taggttttaa attcctctac aaggccattg aatgtgattt ttcttttgag
     5701 aatcgagcaa aaatattgtc tgaagaaatt gtctgtgtta attccatcta actcggttat
     5761 gatttgagcc catattaatt ttacttgttc gagtgtattg tcatcattta ttttagatgc
     5821 gtgtccaagt aaaaagtttt taatgatgtc ggtagcagat aattttaacc ctctgttgtt
     5881 aattgtttca aatagtttgt aagcatcttg agcaagtgat acatctaatc gaataattac
     5941 agcaacgttt attagtttat gataaaataa gtccaaatct tcttcttcat attcggataa
     6001 ccaaaagtca aaacattcta tagcatcctt aagatgtttg tttttaattt catcaaagtc
     6061 tccatcaacc gtaatgattt ggtagtctgg attgtccaag tcacccaata gtattttgtt
     6121 tttcagaacc ttgtcagggc ctttgcattg aagcatttta cggatttcat ttgccgtttc
     6181 ttctcgttca atttttctat atgctttttc tattgacttt aataaaatag taagagttgt
     6241 caatctttgt tgtccatcaa caagttctgg tgtcatgaag cctccattgt aagcaccagc
     6301 atgaagaatt atcattccga aaagatgtcc gtcattgtct tttagtaaat caatgtcgtc
     6361 aaagagtgca cctgtgtgtt gaattttcca agaataacgt ctttgataag atggcacaat
     6421 aaattgttcg tttgagttag caaaaagttg tgcaagggaa agtttgttgg ggactatatt
     6481 catttttttt tagtcgtaat tttttgttgt taaatgtcgt cttaccattg actataacta
     6541 tacttctacg ctattatttt tagccacttc tcctaagaaa gtacctgcct ctgtcatggt
     6601 gcgtttgcct gtttcgaaat caacatgtac taagccaaat ctataatatt gaccaatgtt
     6661 ccattcatgg ttatccatcg tgctccaatg gaaatagcct tgtatcggaa tgccctcttt
     6721 ttgtgcctga tgcacccagc ctaagtattc ttttatactt tcaattctaa attttggatc
     6781 gtgtgtacac acaccgcttt ccgtcataat aataggtttt tttgttttgt tccaatagcg
     6841 atgaaacatt tcataaaaac ctttaggttc atattcccac ataaggtcaa cacgacgacc
     6901 taatttttct aatttgccag gtgagaaaat attatcgaca ggcatcggtc ggaaagacat
     6961 gcgcgcatag taactcaatc cctgaaaatc tacttttaaa aaatgtttgt ggcaatagtc
     7021 cataaaccac cagtcgaaca ctttagcaat cagatgtcct ggaaatattt ctccaacaaa
     7081 ttttactgta ttatgtgaaa tgcctattgg cgctgttgat tttgctttaa tgatatcata
     7141 tgcttcgtcg tgtgctctgc ctatttcttt taacactttt ctagccgtta atattttacc
     7201 ttttttaaaa ggcggaaagc ttcctaaaat ccagccatta gagatataaa cattgggttc
     7261 gttaaatgta ttccatgtat ctactaaatg tccgaatgtg tccactgatt tttgaacaaa
     7321 atctaaccac atttttctat tgccttcttt ttcccagctg ccttcttttt caaaccaaag
     7381 tggattggta aaatgatgca acaccaacat aatgtgtttg cctctacgtt ttagttcttc
     7441 cataaaacgc aaatagggtt cgacttcttt tgggtcgaat ggagcaaaag gtgctttttg
     7501 taattttgcc caatcatgac tcatacgata agcattgcct aattggcata tatattccaa
     7561 atcttcgtct ctatgcaagt cgtgttgcga gcatttatca aagatggttc cgtcgtggca
     7621 tttcaatcct ttccagttgt tctctgatgc tgtttcaatt tgataagctg cggtagaagt
     7681 gccccaaata aatccttcgg gaaattgtaa tttcatgcgt ttagaataat tagtgtatta
     7741 aggttgttgc aataaatctg cgtatataac aaagataaga aacaattttg cattaaaatt
     7801 atttttatct tagttgaggc aatgcaaagc aattctttag aatataataa caatagaatc
     7861 caagtgcgca acagaaaaag tatgttggaa attcggattc ctatcaaaaa aaatggtatt
     7921 gcgctactta tgatgctatt ggcaacagcg gcatggactg taggtttata tgttttggtt
     7981 cgtattgaat tgcatcttca atatttttgg tataaagctg ggctcatttt agccattgcc
     8041 gcatggtttg ttttaggcat gacggttgcc agtttcttta tctggttatt ttttggtcgt
     8101 gagagaattt tagtaaccaa caaatatttg attacagaca aacccttggt attattttat
     8161 agaagaaata tctatgatat tgcagcaata tttaatctta gaaccgatat agaaatttac
     8221 aaagccaatc gcggcggcac atggattgat gaaagcagaa cagtcattcg ctttgacact
     8281 acagacaaat tggtcacttt tgcaagaggc attacaaagc aagaagcgga atttatatta
     8341 ctacaattgg caaaatctac ttacttacag aaacagcaat ttgccgtcga gcagcatttt
     8401 taattcaaca attcttttcc tgcttttttt acaatttgta gtttcacgct cgcctggcta
     8461 tctttcttgg ctttttcatt gaaataataa tacaaattgc ctactatttg tttcgctcct
     8521 tgtttgttgt agttcgattc ttcatacgta taaatgtctt ttaaaaacaa aatacctttt
     8581 tctaaatctg tattgtttgc tttttctaaa tagtcggcaa aacttctaaa attgctgtag
     8641 aatgtatatg gtccgtttaa ccagattgct tttttaaaaa attccatatt atcagcagta
     8701 gtatctttaa aaattctacc aatagccact aataatgaag aattttctgt agaagaaagt
     8761 ttcgtcgcat aagtataagc tttgctttta tcataagtat tgagttgagc aagtgctgca
     8821 ctcaagcaaa tgaaagaaga gtctttttta agcacagttt ccaatacatc tatgctttta
     8881 tttttttcta agcgtgcata tttttgtatt gccttttcgc gcacatgtga gttattgtct
     8941 gtataaatca cttgttgtaa ggctaatgac agttttgttt tattgtcaaa cttatcgatt
     9001 tcaatggcat caagagcttc ttcccgcaaa gtccaatttt tgtcctgtaa ggcttttaac
     9061 aacaattctt gaatggtaac attagctttc tgcaccaatg cgagtgcatt tattgcctct
     9121 aacttatcta caaataaagg tgcattgtag tatttaaaga gattttgcgc cgtgcttaag
     9181 ttttctgttt tttcgcaaac caatacttta tctgcgtcaa agttgactaa ttgtggaacg
     9241 gtgtcagatg gaaaatagaa tgtttgaatt ctgtcagtga tatcgattac tttggtttct
     9301 tttgtgttat tactataaat atctactttg gttggaatgc gaaacgtggg tccatcaata
     9361 ctacgttgcg tttggcgaac tgtgagtgca atggttttat ttttttcgtc gaattgatgt
     9421 gttatgtcaa gaattggatg tcctttatcg agccaccatt gattaaaaaa ccagttcaaa
     9481 tcttcgccag tagtctcttc aaaacatttg cgcaaatcag acaattctgc attattgaat
     9541 ttatattgtt gcaaaaattt atttaaggat ttaaagaaag catcatcgcc aacataataa
     9601 cgcaacatat gtaacacagc gccacctttt tcatatctga tgccatcaaa aacatcagaa
     9661 ggatggacgt aatagtaatt tacaattggt tcatttttgt atttataggt gcgaaaatat
     9721 ttctgtgtag atttatattt aacggcatcg ccttcatctt ttccatctcg aaaatcgtac
     9781 catagatatt cggaataatc tgcaaaagat tcgttaatag ttaagtttgc ccaagactca
     9841 gccgttacca aatcaccaaa ccagtgatga aataattcat gggcaattat ccaatcaaaa
     9901 tcgccatcta ttagctgttg acgatggcac aacattctat caaaataaat gatagccgat
     9961 gtgttttcca ttgcacctgc agtataatcg taagacatta tattgccaag cttgtcccat
    10021 ggataatcaa cacctaaaat attggaaaaa aattccatca tggccggcag gtttttaaat
    10081 atttccgcca catcatcttt atactttgga aaagtataag ccgttacttc tttatcgcgc
    10141 catttatctc tgctgatata gtaatcgcca acgcctaaga aaaacagata cggagaatgc
    10201 ggtttgtctt gcttccaatg atctgttttt gtgccatctc catttggttt gctgtcgatc
    10261 aataaaccat tggatagcga aaccatagcc gtgtcgatag ttacaaaaat ttcttgtgtt
    10321 gatttttggt tggtggcatc taaagtagga aaccaacacg acgcagattc tgattcgcct
    10381 tgcgaccata aatgcattgg tttatacgga tttttatctt ccgtatcgat aaagtacaaa
    10441 cctcgaccat cttcgacctg caagctgtct tgctgataag gcaatgcaac ataatcaatc
    10501 tcaatttcga tttggtcatt ttttgaataa tatttatcca agtctatttt taaaagcaaa
    10561 ttatcgtaag taaacttcag tgttttatac gtttcattag tttttaatgc aaccgcagct
    10621 atttgcatcc atttagcatc tagcactaat tgattttgtt cgtaaaagtg tggctttaag
    10681 gtaagcgtgg cttttccgct tagtgttttt tgtttaaaat taggtgtaag atgtagtttg
    10741 gtgcgttgca gatcaaataa tttttgataa gaagcattat agctcgtgat aaagttttga
    10801 ggtgttacca caatctcttc cagttggctg tgatattttt ttttagactt tttacgtgga
    10861 caatttgcat ataaaacact aaaaaccaac aacatacagg ccaataaacc aaatttacgc
    10921 atagatactt attaacatag tttgaaaaag taaagatatt gaattatccc taattactta
    10981 atatcgtgtt gtgattacaa aattacaaaa tgaattagtt aaactattgt tctatatcaa
    11041 gaaaaagtag tctgttttat ataagataaa aaaagaaaat aaaaacacaa tcatgagata
    11101 taaagtattt tacaaggaag acaatgcaca cgaaattgaa ttagacgagc agcaaataaa
    11161 aatcgatggc aaagcgacag atattgacct tgtaaaacta ttggacaata aatttcatat
    11221 tctgcaaaac cacaagtcat ataatattga ggtgttgcat actgattata atttaaaacg
    11281 attttccata aaagtaaata acaatatcta cgacttaaac ttacagacgg aattagatat
    11341 actactcgaa aaaatgggca tgtccgctgc tggcgatgaa aagatggaca atgtaaaagc
    11401 gccaatgcct ggcttagttt tagatgtatt ggtagaaata ggacaagccg taaaaaaagg
    11461 cgacaatcta ttaatattgg aagccatgaa aatggaaaat attattaaag caagtggaaa
    11521 tggcattgta aaatcaatca aaataaacaa aaaagatgcc gttgaaaaag ggcaactatt
    11581 gattgaaatg gaataattaa atcagtgtga aattattcaa acaatgacat aaaaaattgt
    11641 gttaaaacga caatagcctt tcctctttat cataaatgac atgcacatag ttccacatga
    11701 catagaaaaa tatattgatg cgcacacaag tgccgaaagt gctgttttgc agcagctcaa
    11761 tagaaaaaca caaactgatg tgttaatgcc acagatgttg agtggaaaag tgcaaggtca
    11821 atttttaaaa tttatttcgc aaatggtaca gccaagccgt gtgttagaaa tcggcacttt
    11881 cacaggttat gccgctattt gcttggctga tggcctaagt gctgatggca aacttttcac
    11941 tattgatatc aatgaagagc tggaagacat tgtgcgcacg catgtagaaa aagctggatt
    12001 gcaaaataaa attgtgcaaa tcattggcaa tgctgctcaa gaaataagca agctcaacga
    12061 aacattcgat attgtgttta tagatgcaga caaacaaaac tatagtctat actatgattt
    12121 ggtcatcgat aaagtgcgca atggtggatt tatattggca gacaatgtac tttggagcgg
    12181 aaaaattatt gatgagaaaa aagataaaga cacacaaaaa ttagcagaat ttaacgataa
    12241 agtgcaacaa gataatcgcg ttgaaaacgt tatcatctct atacgcgacg gcattatgat
    12301 gattagaaaa aaatgaaaat gaaaatcaaa tattcgatac tgttttttac ttatttgcta
    12361 ctaagtactg ttagcattaa cgcacaaaaa ttagacacgg cgtctatcat tgcatatatc
    12421 aaactgtata aagatatcgc tgtctctgag atgcagcgca ctggcattcc ggctagcatt
    12481 acactggcgc aaggcataca cgaaagtggt tttggcaaaa gttatttagc acaaaacacc
    12541 aataatcatt ttggtataaa atgtaaagaa gaatggaaag gaaaaacttt caaatacact
    12601 gatgatgcac ctaatgagtg ttttcgcgtg tatgacaaag tagaagattc ttatatcgac
    12661 cattctaact ttttaaaata cagaccacga tatgctgact tgtttttgtt agataaatat
    12721 gattataaag gctggtctta tggtttgaaa aaagctggct atgcgacgaa tccaaaatat
    12781 cctgttatac tcatcaaaac cattgaagat ttcgaattaa cagcatttga caaaggtatt
    12841 aatcctgctt atttacaaac gactttggaa agtataaata agcctatcgc caacgatgag
    12901 caattaaaaa aagacgcacc tgaaataatt cagcatactt ttgaagtgga aaaaaaaact
    12961 gtaattgaat ctgttaataa gcaaaatacg gtgccagtaa gcactaaaaa attaaacaaa
    13021 acaattacaa aaattaataa gtgcaagact gtaaaaattt atccttcaaa aggcgaaaca
    13081 ctcgatttaa ttggcaatgt aatgcgtatc gacaaagaaa aattgttgat ctttaacgat
    13141 gtaaacgacg attatgtttg gaaagatgga caaaccattt ttattcaaca aaagaaaaaa
    13201 tcaaacaaaa cagagcctag ttataaagta aaacaagaag ataatctttg gagcatctct
    13261 caaaagaaag gcgtgcgttt gtcgagttta ttaaaaatga ataaacttag tgccaatgaa
    13321 gaacctgcag tgaaagaaac tattatgcta aaaggaaaaa gcaagctaaa acctgcctta
    13381 cgcagtcaaa aaaatacaga cagcactata aaaaatacta tcgtatcaac accaacaaaa
    13441 aacgtaattt acgatacaat tattcgcgca agagacactg tttatcctaa tatacaaaca
    13501 gatgcaaaat tatctgtcga tagtaataaa atattaggct gggaaagtga aacaaaactc
    13561 ttagaaaaac caagtgccat aacttatcca gtacaatcaa aaccggaaag tattgtaaca
    13621 gtagaaaaaa caaatacaac aactgcgaat atgccaacag tatatccaaa aacaattgac
    13681 tatgcaaaat tgccgaaaag cagcacaggt ttgcacacag ttataaaagg cgatacgatg
    13741 tataacattt gcaaacgcta taatattaca actgcacaat taatggaatg gaataattta
    13801 tcagatcaaa gtgttaaatt aggacaagaa ttaaaaatac aacagtaatt tttgtgctga
    13861 aaaaaattcg aaatgcaaac cattcaaatt aaagataaac tttttaaaca atacatttcc
    13921 gacgcagaaa ttcaacatcg aattcaagaa attgttgccg agctttcatc aaaatatcac
    13981 gacaaaagtc ctgtatttat tgtggtttta cgaggtgcat tcatgtttgc ttctgatatt
    14041 ttaaaaaagt ataatcatcc ttgcgaaata gaatttgtga agctgacctc ttacacaggc
    14101 atggaatcga ctggaaaaat aaatgtgagt ttaccaataa aagaagaagt gattaaagac
    14161 agacatgtca tcattattga agatatcgtc gattctggtt taacgatgga ttttttcagt
    14221 cattacatac aaggcatgca acctgcatca gtggcacttg tatctttttt atacaagcca
    14281 gaaaatcttg taaagcctgt aaatattgac atcgtcggct ttacgattcc agaattattt
    14341 gtaatcggtt atggcttaga ttatgacggt gaaggtcgta atttgagtgc cgtctatcaa
    14401 gtagctgatt aattagcagc aacgattaca gcaccatttt tctttgccca aacgaagttt
    14461 acacctttcg tgtttttatt gatttttgca ccactcatgc gcacaaaaac attgatattt
    14521 ctttcgagca atgtcaatgc attgcctagt gcttccgttt ctaccatttc gggagcgcta
    14581 ttgacttgcg ctgtaatgct agatgtctgt gcataatcaa aaatagcatt tgtgacattt
    14641 gcaccttgca aatttgaacc ttttaaatca gttagtaaga gatttgctgt ttgcaaatta
    14701 gcctctgtca aatcagcaaa ttgcaaaacg gcttcttgca acaatgcttg tgttaaattg
    14761 gctttttgca aattggcata ttgcatttta gcagcaaata aatctgcttt cgataaattt
    14821 gcgccgctta aattcgcatt tgacaaatta caatcatata acttactaga tgacaaatta
    14881 gcgccactta aattggcgcc acttaagttc actccatgta aatcgagttt agataaatca
    14941 gcacctgcta agtcagcatt gaccaagttt ttttcgcctt tcttagccaa ggctaaccat
    15001 tgtgcggcgc ttttttgcgc tatgctattc atcaaaatac atagcaataa ggcatgtaaa
    15061 aagaagattt tttttatcat aatgaattgc gtgttgacta gtgaagatac aaataaagaa
    15121 acaaaaaata aagttaaaaa gtcaaatttt gattgagtat caactgattg tcaaaaatgt
    15181 actgtgccat taaaaaaata gagttttaat aaaaacataa cgacaatttg cttgtttatt
    15241 gatttaatta attgacgttc aatgatttaa aaaacaatga tttcgtttaa taaatttcca
    15301 atttcagaaa aaatattgta tttttgcagt cctttttaaa tctaatacta attaggcatg
    15361 tctaattcat taaacgaata tcaaatacca catgccggat tgaaaaagga tattcatgag
    15421 ttttcttatg aattaacaga aaagttcttt gccaattttg aaaatgcact cattcaaaaa
    15481 tgtgaaattc aagtcaatat tatcctcgat aatagacaag aaccactatt gcttgaaata
    15541 gatatagatg gtattgtgtg gagcgattgt gacaaatgca ctgcgcctat tccaatcgtc
    15601 attcattcta catttgaaat caatgtgaaa tacacaaatg acgaaacgct gaaacacaca
    15661 gacgaaaccg atattatgta tatcgctaga gatgagcaat acatcgatat cgctcaatat
    15721 ttgtatgatt tcgtgcattt atccattcct gttcatgtta tctgtgacca acccggaaaa
    15781 acagattact gcgacatgga aattattggc ttattagaaa aacaacaaac aacatcagat
    15841 acagatccga gatggtctgg tttagataaa ttaaaagaca aattaaatta gaataaaatg
    15901 gcacatccta aacgtagaca atctactaca agacgcgaca aaagacgtac acattacaaa
    15961 atggaagctc caaacgtagt tacatgtaaa gtaactggcg aaagccacct ttctcacaga
    16021 gcttatgttt ctggtaatga tttatactac aacggtaaag tattaatcga aggatacgta
    16081 aaataattta gaccaattgg tgtgagacct aggatgtagg gaaaaacctc aagtctaaat
    16141 ctcatatctc aaaatctaca aaaatggctt ctacatcaga tatcagaaat ggtatgtgta
    16201 tcaacttcaa ctctgacata tacaaagtag tggagttttt gcatgttaaa ccaggaaaag
    16261 gtgcagcatt cgtgcgcact aaattgaaaa gcattaccac aggccgtgta ttagaaaata
    16321 catttcctgc aggtgcaaaa ttagatgaag ttcgcgttga atatcgcgag tatcaatatt
    16381 tatatcctga aaatgatgct tatactttca tgaataatga aacgtatgag caaatcactg
    16441 tttctggaaa tatcatcaat gctcctgaat ttttagaaga aggcatgaac gtaaaaattt
    16501 ctgttcgcgc agaagacgaa atgccattaa cttgcgagct tccagcaact gtaattgtag
    16561 aagttactta cgcagaacct ggtatcaaag gtgatacagc aacaaatact ttgcgtccaa
    16621 gtaccgtttc ttctggtgca aaagttatga tacctttatt tataaatgta ggcgataaaa
    16681 taaaggtaaa cacagaaaca ggtgcttata tggaacgtgt aaaataatac catttcaata
    16741 tgtataaaaa aatgcttcac cttcttgtga agcatttttt ttgcatttaa gtgtagtcac
    16801 attttaatta tgacacatga ttagatgact acattttcaa attattatct atatttgata
    16861 catggatatt gcacaaatca aaaagcttat acaattatta aacgataatc atttaggcga
    16921 attaaaagta caagatggtg attttaaaat tagcatcaaa catagagatt atgcaaaaac
    16981 aagtggtgtt gcgtctgtca tttcagctcc tgtagctacg agcacaccaa gtatagcaca
    17041 agaaactgct actgcaccaa aaaaagaaga aacgccaagt ggccattatc aaacagtaaa
    17101 agcaccgatg attggtacgt tttacagagc tgcaggcgaa ggcaaagaac catttgtaaa
    17161 agtcggcgac aaagtaaaaa aaggcgatgt cttgtgtatt attgaagcaa tgaaattatt
    17221 caacgaaata caaagtgaag tatctggaaa aatagtaaaa gtaatggtgg ataatgcaca
    17281 agccgttgag tatgaccaag cattattttt gatagagtaa
//
LOCUS       DAOQQV010000052        16703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1073_length_16703_cov_7.583133, whole genome shotgun sequence.
ACCESSION   DAOQQV010000052 DAOQQV010000000
VERSION     DAOQQV010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16703
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_16703_cov_7.583133"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLL59_06850"
     CDS             <1..348
                     /locus_tag="PLL59_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMY23562.1"
                     /translation="QEFKNEGFEIIGFPSNDFAKQEPNTAEQAADFCKINYGVSFPIM
                     EKIHVKKGALQHPIFNFLGNNAAGLNLVTHPTWNFQKYLLDKNGLVVDYFVPTTKPDA
                     AKIKSSIKELLKK"
     gene            374..1090
                     /gene="bshB1"
                     /locus_tag="PLL59_06855"
     CDS             374..1090
                     /gene="bshB1"
                     /locus_tag="PLL59_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HMY23563.1"
                     /translation="MKLDILVFAAHPDDVELSCSGTVLKHIQLGYKVGIIDLTQGELG
                     SRGTAEIRAIESKNASQILGIHHRENLGFKDGFFEIDEAHLLKVIQVIRKFQPSIVLV
                     NAKQDRHPDHKRGGDLVSRAAFLSGLVKIETNSDGQQQERWRPNVVYRYIQEQYIEPD
                     FVVDISGFESKKLEAIKAFTSQFYDPNSTEPTTPIAKADYLEFVVARMKQFGTQINVP
                     FGEGFTIERYIGVDNLFQLK"
     gene            complement(1091..2098)
                     /locus_tag="PLL59_06860"
     CDS             complement(1091..2098)
                     /locus_tag="PLL59_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STELLO glycosyltransferase family protein"
                     /protein_id="HMY23564.1"
                     /translation="MSVKSLVITSIAADTLEVLHTYANECKNRNFQFIMIGDTKSPDN
                     FHIDGCDFWSVERQLTLDSKFAKLCPTGHYARKNIGYIIALQNGTVEIVETDDDNLPR
                     EEFWNAKSKIVSAHDIKEQGWVNVYNYFSDKFIWPRGLPLEELQKAAKPLSDFNIKDI
                     ICPIQQGLADENPDVDAVYRLAYPLPLNFNKGMNIALGNNAWSPFNSQNTYWYKEAFP
                     LLYLPAYCSFRMTDIWRSYVAQRISWANDWSVLYHQATVWQERNQHNLMKDFEDEIPG
                     YLNNAKICNELGNLNIKGGQSNMLDDLLTCYDMLTSKGFVGKEEDALVRAWCDDIAKV
                     W"
     gene            complement(2130..2864)
                     /locus_tag="PLL59_06865"
     CDS             complement(2130..2864)
                     /locus_tag="PLL59_06865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY23565.1"
                     /translation="MYLYTNWAATIPMANEEQDFAPFVDLLSRVMDVIGTGKIYFIID
                     NASKDKTLELAQDLSKKDARFEVIWAPDNKNVVDAYLTGFKVAYERGHDIIIEMDAGL
                     SHDPRAIPMYLRVLNEGNEVAFGSRFIKDGSMGDSPFNRRMLSKAGTVLANLLLGTKL
                     YDMTSGFQGFHRDIIGKLLQYPLKSRAHFYQTEVKYLLRKHRTAEVPIHYQAPSPRVS
                     SSAIKNARQTLLYYFIERLKGNSPTI"
     gene            complement(3067..4791)
                     /gene="aceK"
                     /locus_tag="PLL59_06870"
     CDS             complement(3067..4791)
                     /gene="aceK"
                     /locus_tag="PLL59_06870"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HMY23566.1"
                     /translation="MINKDIVHQVACNISEKYDILLAAFNEKTACAKSYFEQRRYQEL
                     FLRQQERYRLYGNYVKEVFADTKDILGESLYNIDYWKVIKIDFTKEVKDKPHRLNSET
                     FYNSITRKVFLDKSFNPDYEFFDYNDYKPMSKYDAEIFDVIECTHFSKVAIKQFLQYF
                     KFDVPFEDLDRDAQLIFDEMAPTIIHQKSNLFLDRIEVLKPVFYRNRGAFIVGRIFYK
                     NWSMPIVIPLLNEEKGIFVDSVIYIPADISIIFSFTRASFFVHTKYPAQLIDYLKAML
                     THKTIGELYDSIGYYRHGKTILYRDLMNYIHNHDDKFLIAPGIKGMVMAVFTLKYYNF
                     VFKIIRDRFEAPKNCTRDFVIKKYEEVEINDRVGRMAYAHLFENLEFPRNLFTHELIK
                     ELMDSCKEAVEFKGDKVIIKHVYIERRMTPLNLYLQNEANPINKGRVILDYGFCVKEL
                     AAANIFPGDLLLKNFGVTRHGRVIFYDYDEIAKVTDCRFRRIPEFNDEDDTRGHHDMI
                     TAEPNDIFPEEFKAFMAPEGPIGEIFLAEHNEIFDPKYWRDIQKRIKAGEYLSFFAYD
                     QYKRFKKA"
     gene            4840..5199
                     /locus_tag="PLL59_06875"
     CDS             4840..5199
                     /locus_tag="PLL59_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HMY23567.1"
                     /translation="MDLETIRSYCLSKKGVTECFPFDEETLVFKVMDKMFLLCNINSD
                     ELAISVKCDSLLAMELRERYEFVTPAYHMNKKMWNTVTYTGEISQFEFLKMIDHSYDE
                     VVKKMSKKMQSVLESMS"
     gene            5196..7040
                     /locus_tag="PLL59_06880"
     CDS             5196..7040
                     /locus_tag="PLL59_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMY23568.1"
                     /translation="MKIWKLKYIAFLIIWFCFNSFSIAYANGDNFKKTININNAFYAE
                     DIYNALVHATTILDDSISIELNIINTSGNAQQLYLSIINPTIDKINIIDEKNKVVVLG
                     DLIPFTKREFKHTNHVFPLFIDKDSSTILHIVIPTIKYKQLNVRIKLATETSFIKTTN
                     HDNFFNGIYIGIYFMFILLMLCVYFFSKNNFFLIYLSIITTSLLLVFQYNGSGFQYLW
                     FYSAFIQKYNTIFISILYFTVHILFISTFFSVQLRKLSKYFFRILLPIVICIVFFIVL
                     QLYRHTTSTLYLYFPYIVMSVFLTYAVLVSLFCIYSYYESKRREVLWVFTGMIFYTAN
                     WLIFINNEFLIFPFFNSWCNFKLYNSDIFVPHLNYIISLLEVIAVTTFIAINYHKLIQ
                     QNNLSSKRLAFLQKRYINTFVLGQEEERGKISEEIEIEISKDILQLKTDLQNSIFKND
                     SKNIIPSVLQVLNTTLTDIDNITDNYVAPNLQQMHLKELIITSTDKLFQEINTSYHLS
                     SIPDKFTLNPVTNINLYRIFQEISNNALKHSQASSISISANIDQKSLQIIIKDNGIGF
                     SENAVASSGIGLLNMESRINSLNGNLYIMSNEKKGTTIHLIMPLSDIL"
     gene            7133..8812
                     /locus_tag="PLL59_06885"
     CDS             7133..8812
                     /locus_tag="PLL59_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMY23569.1"
                     /translation="MRCQPASNYLTLVSNKNDTIIYKLKIKNITAINQELLLEIPIQQ
                     IEKIHVFQLNKNQLIENYNFISKKISQRIYYDRNVIFPINIKAKEINEIYFKYHIDDI
                     HNFSDLNILVWKKDAKITRTEALELTRGVFYGILILYAFVGFVLTVLLNVRNYYYYLF
                     YLVAGGIYLFVKNNLGYEMFWPDYPSIDLFFKKIMLSIYLITSILFLRGFIGDRIKSI
                     KLQITLKYFVYIGAFFVFVSLIAGLLSTPAQNVFIVVQQVFTVVCFITVVLTLIFVYF
                     NTNEKSVVLFTFIFFLSFSFFLFYPQPEFGSGFFGVYFGQIYTYSNAFFIATIFCIFT
                     IYRIKSIMKNNETLKKEVSEINAFYNFSLIEGQQNERLRVGRELHDGVGIMMSAIKMK
                     MSAMQVQDEKEKQRLRYLTHEVDNICDKIRALSHRLLPPTLKKFGLQVALKDVMEDYK
                     IHHQNFTYNFNIPNNLSAVSQQIILDFSENLLDYFSEINPMQLTMSVYVIPSIKEAQI
                     RIQYTGTSLDVNHQYIKSVTSVNDLINGKFTITMLNTWNFKVQMEFPILLQ"
     gene            complement(8815..9756)
                     /locus_tag="PLL59_06890"
     CDS             complement(8815..9756)
                     /locus_tag="PLL59_06890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HMY23570.1"
                     /translation="MDFFTKIKIPKANFSITHQDKIMLIGSCFTENIGNYLVQNCFKV
                     NINPFGIIYNPISIFNVIKYIGTSKIYAVEDLLMNNELYLSTDHHGKFSATSSDEILE
                     NINSSIYIANKHLQACEYIIITLGTAFVYHHIEQNKTVANCHKLPSAHFQKRILSIAE
                     IKNAFNEIQQYINNKKIIFTVSPVRHWRDGAMENLRSKSILIESIYQLIDEYPNCRYF
                     PAYEIMLDELRDYRFYNEDMLHPNELAVKYIWEKFSATYFHVETTEIIKFIDKGNALF
                     AHKIKHSNTQEHRKLEENKLIYKADFMQKYPNIEFPF"
     gene            9836..11104
                     /locus_tag="PLL59_06895"
     CDS             9836..11104
                     /locus_tag="PLL59_06895"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HMY23571.1"
                     /translation="MKLKFFIVTLLFFCLQKSNALVYENRVYAPFIKTVQFFPQGADM
                     AMPILNMNLNDKLELHFDDLSNKPKNYYYTVIQCHSDWTPTTMNAMEYIEGFTEANIN
                     DYDYSNGTRVPYIHYRLTFPNNDMQLNRSGNYVLIVYESNKENPIFVRRFMIAESKVI
                     IDAGIAYPRSLYKRDQFQEVVFKVNYKGFNIDNPQMEIKATVLQNYRWDNAKSDIKPQ
                     FIGMNELNFDYNGVLTFPTAGREFRFFDMRSLRFRGQNIRAFDIRDDENEVYLLFDKP
                     HQRDNYQMTRDLNGQYYIENLDNPYYNTGADYANVHFNFDFNGKLSQGNFYVIGSFNN
                     WICDSSSLLYYDALDKSYTTSILLKQGTYNYTYVLKDEKEKIFDSFTTESYYMDTEND
                     YTILLYYTPFGERYDRLIAIKHLNSLLNKW"
     gene            11118..11666
                     /locus_tag="PLL59_06900"
     CDS             11118..11666
                     /locus_tag="PLL59_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HMY23572.1"
                     /translation="MKHNIEKGDLLVAEPFMKDPSFKRTVTLICEHNTEGSLGLVLSR
                     PSIFRINEMIPNFPAFQGNVFYGGPVGMDRLNYIHAYGDLLEGAFHIKDNLYWNGNFE
                     QLKSLIKAKKIMPQNIKFFAGYAGWEAGQLDEEMQENSWIVCKNYPEIFKMNEYMWHD
                     VLVKMGGKNKFVATLPEDPILN"
     gene            11704..13179
                     /locus_tag="PLL59_06905"
     CDS             11704..13179
                     /locus_tag="PLL59_06905"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HMY23573.1"
                     /translation="MKKFSLLFSIFFTLLYTKAHETVNRCALDEINTHTLLNNPGLAD
                     AYRLEINNGINNITAYDTTIANTDTIYTIKVVVHVVYLNNNKYENIPDNIIQSQIDAL
                     NRDFNALNADTANLRDFFIPFRGNAKIKFELAKTAPNGAVTTGITRTQGSLGNLAGWD
                     PISTILWTALGIEPLKEDYFLLTNSKGKSPWPSGKYLNLYVCDLNKGSRKCASCLTLC
                     DTCGALGGFAYPPSNATNWGPLALDRGKNDGIVIDFRFFGQDNWYAIDSTTQRFKDFY
                     TDGRTTVHEVGHYLGLQHSWGNVLPGITPDGCAVDDFIDDTPEEAAAFASNLVRGTLN
                     PCDTSINTCTKNYLGRDWPDMFENFMDYSTDKCYNLFTKQQVNLMRYNLITRRNGIIS
                     TRELAPTLPTAIKNVSTKEFGVSIFPNPASNQLHILATIPAKEPIFIRVLDMTGKEIL
                     SDKIQNGAQEKTFSIQSIPAGIYLIQCYNESFSTFEKLVKE"
     gene            13308..14687
                     /locus_tag="PLL59_06910"
     CDS             13308..14687
                     /locus_tag="PLL59_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY23574.1"
                     /translation="MIYDFIVVGSGSGGGAIAYYLNKSGAKVLLLEAGHFYRKDTFPK
                     TEAETSAHLYWGGGIEFGKDGRMGFLRTKMVGGSSIVYQCLMDRFDDIAFNDWKNESG
                     VDWFNAQEMDKHYSQVESDMVLHTFNADEMRPNGKLFADACDKLGYKWGFLRRGQSDC
                     APEKDNDCTGCFGGCFRDSKQSSLVAFIQKAEKEGLEIQANTEILKVEPNSNTCKIHG
                     FQNGKAISFEGKKVILSGGAFGSTRMMTLSGFKSKLPALGNNFSSHPQFMWLGFQNEL
                     VDAHKGMFQSVASKDDNFRKQGFKLENVFASPISIGMLMNESGVEHQRLMKQYRYMQC
                     IEVAVRDENVGKILCDNKGKLTIEKPLTDQDKLRRDKGHQAIENILNMSGAKEIYKSN
                     FYFGLHLMGGCVMGTDATKSVVNPDFQVHGEKTLYIADSSIYPNAPGINPGLTIKSLG
                     HRLATQLTK"
     gene            14689..15093
                     /locus_tag="PLL59_06915"
     CDS             14689..15093
                     /locus_tag="PLL59_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23575.1"
                     /translation="MANFFSTSATKGLLKVGEVYCPKNGEFPSFKETAGTAYLNNLVK
                     NVPQDDFSSLSLVLSIFSFLPQFMVAWIIEFCRKAQYNTSDNLLYNNLRQLNLGLRGL
                     CFSIYYSEFTNPNYKGKKPLEILDYSLNRVTE"
     gene            15214..16002
                     /locus_tag="PLL59_06920"
     CDS             15214..16002
                     /locus_tag="PLL59_06920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY23576.1"
                     /translation="MPSLFQIEEFIHYWITSFNKYKIHSPYIFKLVTEVFEKEINYAD
                     FKRIEDLRDSLKENDNEIEVVDLGAGSRAFKTHRRKISKIVRRSVKKEKYGHLLYMMI
                     RHAKANAILELGTSLGITTAYMASAHQNTRVITVEGSESIAKLAQENFDKLKLNNIQL
                     VVGNFDDVLKNICDEHKVFDLIFIDGNHREVPTINYFNTVLPCCHNDTILLFDDIHWS
                     EEMQEAWEYIKNHEKVTCTIDIFEMGIVFLRKEFRQKQHMQITY"
     gene            complement(16012..16545)
                     /locus_tag="PLL59_06925"
     CDS             complement(16012..16545)
                     /locus_tag="PLL59_06925"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMY23577.1"
                     /translation="MRTVAETLNIQTELIMPAHTNPIGNLMGGHLLSWMDVCAGISAI
                     KHSGGISVTAGLDNVSFNLPIKMGDIVILKSFVTRAFNTSMEVICEVFIKNIKTQEEV
                     KSHEAFFSFVGIDFQGKPIRCAEVLPVSDREKELYESALRRRELRLINAGKLSPKDAV
                     LLRKELFDDIEDSSNIL"
BASE COUNT         5678 a         2657 c         2694 g         5674 t
ORIGIN      
        1 caagaattta aaaatgaagg cttcgaaatt ataggttttc caagcaatga ttttgccaag
       61 caagaaccaa acacagcaga acaagccgct gatttttgta aaatcaatta tggtgtttcg
      121 tttccgataa tggaaaaaat tcatgtaaaa aaaggagcgc tgcaacatcc aatttttaat
      181 tttttaggca acaatgcagc tggtttaaat ttggtgacgc atccaacatg gaattttcaa
      241 aaatatttgc ttgataaaaa tggacttgtc gtcgattatt ttgtgccgac caccaaacct
      301 gatgctgcca aaataaaatc ttctattaaa gaattactta aaaagtaagt aattttgtag
      361 cgcaataaat gttatgaaat tagatatttt ggtttttgca gcgcatcctg atgatgtaga
      421 attgtcgtgc agtggaactg tgctaaaaca cattcaatta ggctataaag taggcatcat
      481 tgatcttaca caaggtgaat taggctcgcg tggtacagca gaaattcgtg ccatagaatc
      541 aaaaaatgct tcgcaaatat taggcattca tcaccgtgaa aatttaggat ttaaagatgg
      601 ttttttcgaa attgatgaag cacatctgct gaaagttatc caagtaatca gaaaatttca
      661 accttccatc gttttggtta atgcaaaaca agacagacat ccagatcata aacgaggtgg
      721 cgatttggtg tcgcgagcag catttttgag tggtttagta aagattgaga caaattctga
      781 tgggcaacaa caagaaagat ggcgcccgaa tgttgtgtat cgatatatac aagaacaata
      841 catagagcca gattttgtgg tagatatttc aggttttgaa tctaaaaaat tggaagcgat
      901 aaaagctttt acttcgcaat tttatgatcc taattcaaca gaaccaacta caccaattgc
      961 caaagccgat tatttagaat ttgtagtagc acgaatgaaa caatttggca cacaaattaa
     1021 tgtgcctttt ggagaagggt ttactatcga gcgttatatt ggcgtcgata atttatttca
     1081 attgaaataa ttaccaaact tttgcaatat catcacacca agcacgcact aacgcgtctt
     1141 cttctttgcc tacaaaacct ttagaagtca acatatcata gcaggtcaat aaatcatcta
     1201 acatattgga ttgtccacct ttgatattca aattgcctaa ttcattgcaa atttttgcat
     1261 tatttaaata acctggaatt tcgtcttcaa aatctttcat aagattatgc tgatttcttt
     1321 cttgccaaac cgttgcttga tgataaagca cagaccaatc atttgcccaa gatatacgtt
     1381 gcgccacata gcttctccaa atatcggtca tacggaaact gcaataagct ggcaagtata
     1441 aaagtggaaa tgcttcttta taccaataag tattttggct attgaaagga ctccatgcat
     1501 tattgcccaa agcaatgttc atgcctttat taaaatttaa aggtaatgga taagccaatc
     1561 tatatacggc atcaacatca ggattttcat cagcaaggcc ttgttgaata ggacaaatga
     1621 tatctttaat gttaaaatcg gagagtggtt ttgctgcttt ttgtagttct tcgagtggca
     1681 aaccacgtgg ccatataaac ttgtcgctga agtagttata tacgtttacc caaccttgtt
     1741 ctttaatgtc atgagcactt actattttcg attttgcatt ccaaaattct tcacgtggca
     1801 aattgtcgtc atcagtttct acaatctcaa ctgttccatt ttgaagcgca atgatataac
     1861 ctatattttt tcttgcataa tgtcctgttg gacaaagttt tgcaaatttt gaatcaagtg
     1921 ttaattgacg ttcaacagac cagaaatcac aaccatcgat atgaaaatta tctggcgatt
     1981 tagtatcacc aatcataata aactggaaat tacgattctt acattcattg gcgtaagtgt
     2041 gtagcacttc caaggtatct gctgcaatag atgtaataac taatgattta actgacatat
     2101 tgtaatgtat ttattttgaa ttatttatgt taaattgttg gtgaatttcc ttttaatctt
     2161 tcaataaaat aatagagcaa agtttgtcta gcatttttga ttgctgaaga cgaaacacgt
     2221 ggagaaggtg cttgataatg aataggcacc tcagctgttc tatgttttct gagtaaatat
     2281 ttaacttcag tctggtaaaa atgagctctc gatttcagtg gatattgtaa taatttaccg
     2341 attatgtcgc gatggaaacc ttggaaacca gatgtcatat cgtataactt tgtgcctaac
     2401 aataagttag ctaaaacagt tccagcttta cttaacattc gtctgttaaa aggagaatcg
     2461 cccatggagc catcttttat gaatcttgag ccgaatgcaa cttcatttcc ttcatttaaa
     2521 acgcgtaaat acatcggaat tgctcgtgga tcatggctta atcctgcgtc catttcaata
     2581 ataatatcat gaccacgttc ataagctact ttaaaaccag ttaagtaagc atcaacgaca
     2641 ttcttattat ctggcgccca gattacttca aatcgtgcat cttttttaga taaatcttgt
     2701 gcaagttcta aagttttatc ttttgatgca ttgtctatga taaaataaat ttttccagta
     2761 ccgatgacat ccattactcg agaaagtaaa tctacaaaag gagcaaaatc ttgctcttca
     2821 tttgccattg gtattgtagc tgcccagttt gtatataagt acatgcgtat taggttagtg
     2881 gtagtttgcg atttatctac ttaacataat ataaattata ggacaaatta tatagtgtta
     2941 atgttgataa tcagcggttt ataattagtt taattttgca aaaatacaaa catactaatt
     3001 atatcgaatt tatacaagtt gctaaaaaat ttatgacaaa tctgatataa ttaagtcatt
     3061 gtcaacttat gcctttttaa atcttttata ttggtcataa gcgaaaaagc taagatattc
     3121 tccagccttt attcgttttt gaatatcacg ccaatatttc ggatcaaaaa tttcattatg
     3181 ctctgctagg aaaatttctc cgattggacc ttctggcgcc atgaatgctt taaattcttc
     3241 agggaaaata tcgtttggtt cggctgtaat catgtcgtga tgtccacgtg tatcgtcttc
     3301 gtcattgaat tctggaattc gacgaaaacg gcaatccgta acttttgcaa tttcatcata
     3361 atcgtaaaaa ataacgcgtc catgtctggt aacaccaaaa tttttcaata ataaatcacc
     3421 tggaaatata tttgctgccg ctaattcttt tacacaaaat ccataatcta aaattactcg
     3481 tcctttgttt atcggattgg cttcattttg taaatacaaa tttaaaggtg tcattctgcg
     3541 ctcaatatat acgtgtttta taatcacttt atcaccttta aactcgacag cttctttgca
     3601 agaatccatt agttctttaa tcaactcgtg tgtaaataga ttgcgtggaa attctaaatt
     3661 ttcaaataaa tgagcatacg ccattctgcc tacgcggtca ttaatctcca cttcttcata
     3721 ttttttgata acaaaatcac gcgtacaatt tttgggtgct tcaaatctgt cgcgaataat
     3781 tttgaaaaca aaattgtaat atttaagtgt aaaaactgcc attaccatac cttttatacc
     3841 tggtgcaatc agaaatttat cgtcgtgatt atgtatgtag ttcatcaaat cgcgatataa
     3901 aatagttttc ccgtgtcgat aatagccaat agaatcatac aactcgccga tagttttatg
     3961 tgtcagcatg gcttttaagt aatcaatcag ttgtgctgga tatttggtgt gtacaaaaaa
     4021 cgaagctcta gtgaagctaa aaatgatact aatatcagct ggaatataga taacagaatc
     4081 tacaaaaatg cctttttctt catttaataa aggaattaca attggcatcg accaattttt
     4141 ataaaaaatt ctaccgacaa taaaagcacc tcgattgcga tagaaaactg gcttgagaac
     4201 ttctatccta tctaaaaata aattagattt ttgatgaata attgtcggcg ccatttcatc
     4261 aaaaataagt tgtgcatcac ggtcaagatc ttcgaaagga acatcaaatt taaaatattg
     4321 cagaaattgt ttgatggcaa ctttcgagaa atgtgtacat tctataacat caaaaatctc
     4381 tgcgtcatat tttgacatgg gtttataatc attataatcg aagaactcgt aatctggatt
     4441 aaaacttttg tcgagaaata ccttacgtgt tatggagtta taaaatgttt ctgaattgag
     4501 acgatgtggt ttgtctttta cttctttggt gaaatcgatt tttataactt tccaatagtc
     4561 aatattgtat aagctttcgc ctaatatatc ttttgtgtct gcaaaaactt ctttgacata
     4621 attgccatat agtcgatagc gttcttgttg gcgcaagaat aattcttgat aacgtctttg
     4681 ttcaaaatag gatttagcgc aagccgtttt ttcattaaaa gcagctaata aaatatcgta
     4741 tttttctgaa atattacaag caacttgatg tactatatct ttgtttatca ttattatagt
     4801 atttttagaa ttgtttatct gtaaatgtaa aagtaaaaaa tggatttaga aaccatcagg
     4861 tcatattgtt tatcaaaaaa aggcgtaaca gaatgttttc catttgatga agaaaccttg
     4921 gtttttaaag taatggacaa gatgtttttg ttatgtaata tcaatagtga tgaattagca
     4981 atttcagtaa aatgcgatag tttattagca atggaattgc gagaacgcta tgaatttgtt
     5041 actcctgcgt atcatatgaa taaaaaaatg tggaatactg taacttatac aggtgaaatt
     5101 tcacagtttg aatttcttaa aatgatagat cattcttatg atgaagtggt aaaaaaaatg
     5161 tctaaaaaaa tgcaatctgt tttggaatca atgtcatgaa aatttggaaa ctaaaatata
     5221 ttgccttcct cataatttgg ttttgtttca attccttttc catcgcttat gcaaatggag
     5281 ataattttaa aaaaacaatt aatataaata atgcatttta tgcagaagat atttacaatg
     5341 cattggtaca tgcaacaaca attttagacg acagtatttc tattgagctc aatattatca
     5401 acacttctgg caatgcgcag caattatatc taagcattat aaatcccaca atagacaaaa
     5461 taaatattat tgatgagaaa aataaagttg ttgtattagg tgatttgatt ccttttacta
     5521 agcgtgaatt taaacatacc aatcatgttt ttcctttgtt tatagataaa gacagcagca
     5581 cgatattaca cattgttatt cctacaatta aatacaaaca attaaacgtt agaattaaac
     5641 ttgctactga aacttcattt atcaaaacta ccaaccacga taattttttt aatggcattt
     5701 atataggtat ttatttcatg tttatcttgt taatgctgtg tgtatatttc tttagtaaaa
     5761 ataatttctt tctaatttac ttatccatta tcaccacttc tctcctattg gtttttcaat
     5821 ataatggctc tggttttcaa tatttatggt tttattccgc atttatacaa aagtataata
     5881 ccatttttat ttctatcctt tattttactg tgcatatttt atttatcagc actttttttt
     5941 cagttcaact acgaaaatta tctaaatatt tttttagaat tttgttgccg atagttattt
     6001 gtattgtctt ttttattgta ttgcagctct atagacacac gacgagcaca ctgtatttat
     6061 attttccgta tatagtaatg agtgtttttt tgacgtatgc agtactggta tcattgtttt
     6121 gtatatattc ttattacgaa tcaaaacgac gtgaagtact ttgggttttt acaggtatga
     6181 tattttacac agccaattgg ttgattttta ttaataatga atttttgatt tttccatttt
     6241 tcaattcgtg gtgtaatttt aaattataca atagtgatat ttttgttcct catttgaatt
     6301 atattatttc tctgctcgag gtgattgcag ttactacttt tattgcaata aattatcaca
     6361 aactcattca gcaaaataat ttgtcttcta aaagattggc ttttttacaa aaacgttata
     6421 tcaatacatt tgtactaggt caggaagaag agcgtggaaa aattagcgaa gaaattgaaa
     6481 tagaaatttc aaaagacata ttgcaattaa aaacagattt acaaaatagc atatttaaaa
     6541 atgattcaaa aaatataata ccttctgtgc tgcaagtgct taatacaaca ttgacagata
     6601 tcgataatat tacagataat tacgttgcac ctaatttgca gcaaatgcat ttaaaggaat
     6661 tgataatcac ttctactgat aagctttttc aagagataaa tacaagctat catttatctt
     6721 ctattcctga taaatttact ttaaatccgg tgacaaacat aaatctatat cgcatttttc
     6781 aagaaatttc aaataatgca ttgaagcatt ctcaagcttc atctatttct atttcagcaa
     6841 atatcgatca aaaatctttg cagataatta ttaaagataa tggaattgga tttagtgaaa
     6901 atgcagtggc atcaagcgga attggcttgc tgaacatgga atcgagaatt aatagcttaa
     6961 acggaaattt atatattatg tctaatgaaa aaaaaggaac aaccattcat ttaatcatgc
     7021 cactttctga tatactttga aaaatagtaa acacatatta tttctattga ttgttctgta
     7081 ttcgtttaat tgtttacagg caaataataa tgtctatata attgataaat cattgcgttg
     7141 tcaacctgcg agtaattatc tcacattggt aagcaataaa aacgacacga ttatttataa
     7201 actaaaaata aaaaatatta ctgctataaa tcaagaatta ttacttgaga ttccaattca
     7261 gcaaattgaa aaaattcatg ttttccaatt aaataaaaat caacttattg aaaattataa
     7321 ttttatttct aaaaaaatta gtcaacgtat ttactacgat agaaatgtca ttttccctat
     7381 taatattaaa gcgaaagaaa tcaatgaaat ttattttaaa tatcacatcg atgacattca
     7441 taatttttct gacttgaata ttttagtttg gaaaaaagat gctaaaatta ctagaactga
     7501 agcactcgaa ttaacacgtg gtgtttttta tggcattcta attttatacg cattcgttgg
     7561 ttttgtactt accgttttat taaacgttcg taattattat tactatttat tttacttagt
     7621 agctggtgga atttatcttt ttgtgaaaaa taatttaggc tatgaaatgt tctggcctga
     7681 ttatccttct atcgatttat ttttcaaaaa aataatgcta tctatttatt taattacatc
     7741 aattcttttt ctacgtggtt ttattggaga tagaataaaa tcaattaaac tacaaataac
     7801 actgaaatat ttcgtttaca ttggagcatt ttttgtcttt gtgtccctga ttgctggctt
     7861 attgagcacg ccagcacaaa atgtatttat agtagtacag caagtgttta cagtagtttg
     7921 ttttattacg gttgtactta ctttaatatt tgtatatttt aatacaaatg aaaaatctgt
     7981 tgtgttattt acgtttatat ttttcttgtc tttttcattc ttcttatttt atccacaacc
     8041 tgagtttggc agtggtttct ttggcgtata ttttggtcaa atatacacct acagtaatgc
     8101 attttttata gcaacgattt tttgcatttt cacaatttat agaataaaat ctataatgaa
     8161 aaacaacgaa acattaaaga aagaagtgag tgaaatcaat gctttctata atttttcatt
     8221 aattgaaggc caacaaaacg agcgtttacg cgttggtaga gaattgcatg atggcgttgg
     8281 tataatgatg tcggccatta aaatgaaaat gtcggccatg caagtgcaag atgaaaaaga
     8341 aaaacaacgc cttcgatatc ttacgcatga agttgataat atttgcgata aaatcagagc
     8401 attgtctcat cgacttttgc cgcctacgct aaaaaaattt ggcctacaag ttgcactaaa
     8461 agatgtaatg gaagattata aaatacatca tcaaaatttt acttacaact ttaatattcc
     8521 aaataattta tctgctgttt cgcaacaaat catattggat tttagtgaaa atctactcga
     8581 ttatttttca gaaataaatc ctatgcaact caccatgagt gtttatgtaa taccatccat
     8641 caaagaagca caaatcagaa ttcaatatac aggaacaagc ttagatgtta atcaccaata
     8701 tataaaatca gtaacttctg ttaatgattt aattaatggc aagtttacga taacgatgtt
     8761 aaatacttgg aattttaaag tgcaaatgga atttccaatt ttattacaat agcattaaaa
     8821 tggaaattca atgtttggat atttctgcat aaaatccgct ttatatataa gtttattttc
     8881 ttctaattta cgatgttctt gcgtgttgct atgcttaatt ttatgagcaa ataaggcatt
     8941 tcctttatcg atgaatttaa ttatttctgt agtttcaaca tgaaaatatg tagcagaaaa
     9001 tttctcccaa atatacttta cagcaagctc atttggatgt aacatatctt cgttataaaa
     9061 tctataatct cgtaattcat caagcataat ttcataagca ggaaaatatc tacaatttgg
     9121 atattcatca ataagttgat aaatactttc gattaaaata cttttgctac gtaggttttc
     9181 catagcacca tcgcgccaat gtcgaacagg cgaaaccgta aatattattt tcttattatt
     9241 gatatattgt tgaatttcat taaatgcatt ttttatttca gcaatactta aaattctttt
     9301 ttgaaaatgt gcactcggaa gtttatgaca attcgccact gttttatttt gctcaatatg
     9361 atgatacaca aaggcagttc ctaatgtaat gatgatatat tcacatgctt gtaagtgctt
     9421 atttgcaatg tatattgaac tatttatatt ttctaaaatt tcatcacttg aggtagcaga
     9481 aaatttacca tgatgatcgg tcgataaata caattcatta ttcatcaata aatcttctac
     9541 ggcatatatt ttcgaagtgc caatatattt gataacatta aaaattgaaa tcggattgta
     9601 gattattcca aatggattta tattgacttt aaaacagttc tgtacaagat agtttccaat
     9661 gttttcagta aaacaagagc caatcaacat tattttatct tgatgtgtga ttgaaaaatt
     9721 agctttaggt atttttattt ttgtaaaaaa gtccattttt gatgatataa atctattatc
     9781 tttcacaaag atactgaatt tccatttttg gaaagatatt tgctatactc aaatgatgaa
     9841 gcttaaattt tttatagtca ccttactctt tttttgctta cagaaaagca atgcacttgt
     9901 ttatgaaaat agagtctatg cgccatttat taaaacggtg caatttttcc ctcaaggtgc
     9961 cgatatggca atgccgatct tgaatatgaa tcttaacgat aaactcgaac ttcacttcga
    10021 tgatttatct aataaaccaa aaaattatta ttatacagtc atacaatgcc acagcgattg
    10081 gacgccaacc accatgaatg caatggaata tattgaagga ttcacggaag caaacataaa
    10141 tgattacgat tattcaaatg gaacgagagt gccgtatatt cattatcgtt tgacttttcc
    10201 caataatgat atgcagctta atcgttctgg caattatgtc ttaattgtat atgaaagcaa
    10261 taaagaaaat ccaatttttg ttcgccgttt tatgattgcc gagtcgaaag ttataattga
    10321 tgcgggcatt gcttatccac gtagcctcta caaacgcgac caatttcaag aagtagtttt
    10381 caaagtcaat tacaaaggct ttaatattga caatccacaa atggaaataa aagcaacagt
    10441 tttgcaaaac tatagatggg acaatgcaaa aagcgatatc aagccacaat ttatcggcat
    10501 gaatgagctt aattttgatt acaatggtgt cctcaccttt cctactgctg gcagagaatt
    10561 tagatttttt gatatgcgtt ctctgcgctt tcgtggacaa aatattagag cattcgatat
    10621 tcgcgatgac gaaaatgagg tgtatctgtt atttgacaaa ccacatcaac gcgataatta
    10681 tcaaatgact cgcgacttaa atggtcaata ttatatcgaa aatttagata atccatatta
    10741 caacacaggc gccgattatg ctaatgtgca ttttaatttt gattttaatg gcaaactttc
    10801 gcaaggcaat ttttatgtca tcggttcctt taataattgg atctgcgatt cgtcttccct
    10861 actgtattat gacgctttgg acaaaagtta tactacctca attttattaa aacaaggaac
    10921 ctataattat acttatgtgt tgaaagatga gaaagaaaaa atatttgatt cttttacaac
    10981 agaaagctat tatatggaca cagaaaatga ttatactatt ctattgtatt atacaccttt
    11041 tggcgaaaga tatgatagac ttattgccat aaaacatcta aattcgttat tgaataaatg
    11101 gtaaatgaaa tatgcctatg aaacacaaca tagaaaaagg agatttattg gtagctgagc
    11161 catttatgaa agacccaagc ttcaaaagaa cagttacgct catctgtgaa cacaatacag
    11221 aaggttcttt aggcttggtg cttagcagac catctatttt tcgaatcaat gaaatgatac
    11281 caaattttcc ggcttttcaa ggcaacgttt tttatggcgg accagttggc atggataggt
    11341 taaactatat tcacgcttat ggcgatttac tcgaaggcgc gtttcatatt aaagataatt
    11401 tatattggaa tggcaacttt gaacaattaa aatctttgat aaaagccaaa aaaattatgc
    11461 cgcaaaatat aaaattcttc gcaggatatg ccggttggga agcaggtcaa ctcgatgaag
    11521 aaatgcagga aaatagctgg attgtatgca aaaactatcc tgaaattttc aaaatgaatg
    11581 aatatatgtg gcacgatgtg ttagtaaaaa tgggcggaaa aaataaattt gtcgccacat
    11641 tacctgaaga tcctattctg aattaaaaag aaagtttcgt aactttgttt aaatagccag
    11701 gttatgaaga aattttcttt acttttttca attttcttta ctttattata tacgaaagca
    11761 cacgaaactg taaaccgttg tgctttagac gaaataaata cacatacttt attaaacaat
    11821 cctggtttag cagatgcata tcgattagaa attaataatg gcatcaacaa tataactgct
    11881 tacgacacta cgattgccaa tactgatacc atttacacta tcaaagttgt agtacatgtt
    11941 gtctatctca acaataataa atacgaaaat ataccagata atatcattca atctcaaatc
    12001 gatgcgctaa atagagattt caatgcttta aatgctgaca ctgccaatct tcgcgatttc
    12061 ttcattccat ttagaggcaa tgcaaaaatc aagtttgaat tggccaaaac agcacccaat
    12121 ggtgctgtaa ctacaggtat tacacgcacg caaggaagct taggcaatct tgctggctgg
    12181 gatccaatca gcactatttt atggacagca ttgggcatcg agccactcaa agaagattat
    12241 ttcttactta ctaactcaaa aggaaaatct ccatggcctt ctggtaaata tttaaactta
    12301 tatgtttgcg accttaataa aggcagcaga aagtgtgcat cgtgtctaac gctttgtgat
    12361 acttgcggtg cattaggcgg ctttgcttat ccgccttcaa atgctactaa ctggggacca
    12421 cttgctttag atagaggaaa aaatgatgga atcgtaattg acttcagatt ttttgggcaa
    12481 gacaattggt atgcaataga ttcaactacg caacgcttta aagatttcta tactgatgga
    12541 agaacaactg ttcacgaagt aggccactat ttaggtttac aacatagttg gggaaatgta
    12601 ttgccaggta tcacacctga tggctgtgcc gtcgatgatt ttatagatga tacaccagaa
    12661 gaagctgccg cttttgccag taatctggtg agaggcactc tcaatccttg tgacacatcg
    12721 attaatactt gcaccaaaaa ttatttagga agagattggc cagatatgtt tgaaaatttt
    12781 atggactatt caacagacaa atgctataat ttatttacaa aacaacaggt aaacttaatg
    12841 cgctacaatt taatcactcg tagaaatggt attatttcca cgcgtgaact agcacccacc
    12901 ctaccaactg ccattaaaaa tgtttctact aaagaatttg gtgtttcaat ttttccaaat
    12961 ccagcatcaa accaattaca cattctcgcc actatacctg cgaaagagcc aatatttatt
    13021 cgtgtgctcg acatgacggg caaagaaata ttgtcggata aaatacaaaa tggagcgcaa
    13081 gaaaaaactt tttctataca atctattcct gctggtattt acttgattca atgttataat
    13141 gaatcatttt ctacttttga gaagttagta aaagagtaat tcatcattag atgatttgaa
    13201 ttatctttta aacaagatta acaatttatt tacagctatt aactgtttta tactacaaaa
    13261 taataggtat atttaagcaa ttatctaaac agtaacccac aattatcatg atttacgatt
    13321 ttattgttgt tggctcaggt tctggaggtg gcgcaattgc ctattatctc aataaatctg
    13381 gtgcaaaagt gcttttgctt gaagctggac atttttacag aaaagatact tttccaaaaa
    13441 cagaagcgga aacgtcagcg catttatatt ggggcggagg catcgaattt ggaaaagatg
    13501 gccgtatggg tttcttgcgt actaaaatgg taggtggttc atctatagta tatcaatgtt
    13561 tgatggatag atttgatgat attgccttta atgattggaa aaatgaaagt ggcgttgatt
    13621 ggtttaatgc gcaagaaatg gataaacatt attctcaagt cgaaagcgac atggtattgc
    13681 atacattcaa tgcagatgaa atgcgaccaa acggcaaatt attcgctgat gcatgcgata
    13741 aattaggcta taaatgggga tttttacgtc gtggacaaag cgactgtgca ccagaaaaag
    13801 ataatgattg cactggctgt tttggtggtt gctttagaga ttctaaacaa agttctttag
    13861 tagcatttat tcaaaaagca gaaaaagaag gcttggaaat acaagccaat acggaaatat
    13921 taaaagtaga acccaatagc aatacttgca aaattcatgg ttttcaaaat ggaaaagcga
    13981 taagttttga aggcaaaaaa gttatcttat ctggtggtgc ttttggctct acgcgtatga
    14041 tgacgctttc aggatttaag tctaaattgc ctgctttagg aaacaacttc tcatcgcatc
    14101 cacaatttat gtggttagga tttcaaaacg aattggtaga tgcacataaa ggcatgtttc
    14161 aatcagtagc atcaaaagat gacaacttca gaaagcaagg ttttaaatta gaaaatgttt
    14221 ttgcttcgcc aatatctatt ggtatgttga tgaatgagtc tggcgtagaa catcagcgac
    14281 taatgaaaca atatcgttat atgcagtgta ttgaagtggc tgtgcgcgat gaaaatgtgg
    14341 gtaagatact atgtgataat aaaggaaaac tcaccattga aaaaccattg accgatcaag
    14401 ataaattacg cagagacaaa ggacaccaag ccattgaaaa catcttaaat atgtcaggtg
    14461 ctaaagaaat ctataaatct aatttctact ttggtttgca ccttatgggc ggctgtgtta
    14521 tgggcactga tgcgactaaa tctgttgtaa atcctgactt tcaagtgcat ggagagaaaa
    14581 cgctttatat tgcagattcc agtatatatc caaacgcacc tggaattaat ccaggattaa
    14641 ctatcaaatc attaggtcat cgtttagcaa cacaattaac aaaataaaat ggcaaacttt
    14701 ttctctacca gcgcaacaaa aggacttcta aaagttggcg aagtatattg tccaaaaaat
    14761 ggagaatttc catcatttaa agaaacagca ggcacagcat acttaaataa tttagtaaag
    14821 aatgtaccac aagatgattt ttcatcctta agtttggtct tgtctatttt ctctttcttg
    14881 ccacaattta tggtagcctg gatcatcgaa ttttgtagaa aagcacaata caacactagt
    14941 gacaaccttt tgtataataa tttacgtcaa ttgaatttag gattacgtgg tttatgtttt
    15001 tcgatatatt attcagagtt taccaatcca aattataaag gcaaaaagcc gcttgaaata
    15061 ttggactatt cactcaatcg agttacggaa taattttttt aaaaagaata attcctataa
    15121 aagcaacatt aaatattttg tagcatgcac ttattaataa tggtggaaaa ggtttttccg
    15181 cataattaat atagaatatg tatttttgtt caaatgccaa gtcttttcca aatagaagaa
    15241 tttattcatt attggattac ctctttcaat aagtataaaa tacactcgcc ttatattttc
    15301 aaattagtta ctgaagtttt tgaaaaagaa atcaactatg ctgattttaa acgcatcgaa
    15361 gaccttcgtg attcattaaa agaaaatgac aacgaaattg aagttgtaga tttaggcgct
    15421 ggttctcgtg catttaaaac acatcgcaga aaaatcagca aaattgttcg tcgtagtgta
    15481 aaaaaagaaa aatacggaca tctactctat atgatgattc gacatgctaa agcaaatgcc
    15541 attttagaac tcggcacttc tttaggtatt actactgctt acatggcatc tgctcatcaa
    15601 aatacaagag taattactgt tgaaggaagt gaatctattg ccaaattagc gcaagaaaat
    15661 ttcgataaat taaagctgaa caatattcaa cttgtagtcg gaaattttga tgatgtatta
    15721 aaaaacattt gtgatgaaca caaagtattt gatttaattt ttattgatgg caatcatcga
    15781 gaagtaccaa ccattaatta tttcaatact gttttaccgt gctgccataa cgacaccatt
    15841 ttattatttg acgatattca ttggagtgaa gaaatgcaag aagcttggga atacatcaag
    15901 aaccacgaaa aagtaacctg tacaatcgat attttcgaaa tgggaattgt gtttttaaga
    15961 aaagaattcc gacaaaaaca acacatgcaa attacctatt agcaataaat gttacaatat
    16021 attcgaactg tcttctatgt cgtcgaataa ttctttccga agcaaaacag catctttagg
    16081 acttaatttt ccagcattta ttaagcgcaa ttcacgtctg cgtaaagcac tttcatatag
    16141 ttctttttcg cgatctgata ctggcaatac ttcagcgcat cgtataggct ttccttgaaa
    16201 atcgatgcct acaaatgaaa aaaatgcctc gtgtgatttt acttcttctt gtgttttaat
    16261 atttttgata aacacttcac aaatcacttc catcgacgta ttaaatgcac gagtaacaaa
    16321 ggattttaga attacgatat cgcccatttt tattggcaaa ttaaaagaga cattgtcgag
    16381 ccctgcagtt acagaaatgc caccactgtg tttgatagca gaaatgccag cgcaaacatc
    16441 catccagctg agtaaatgtc cacccattaa attgccaatt ggattagtat gagcaggcat
    16501 aataagttca gtttgtatgt ttaaagtttc agctaccgtt cgcatttata tatagatttt
    16561 attttcactt tgatattaaa atatttggct tatttttgca gtcaaatcgt tacaaatata
    16621 gcacttgtaa cgacattttt caaagattgg ctgcgtagtt caactggata gaatatcaga
    16681 tttcggctct gagggttgag ggt
//
LOCUS       DAOQQV010000053        16553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1087_length_16553_cov_8.096315, whole genome shotgun sequence.
ACCESSION   DAOQQV010000053 DAOQQV010000000
VERSION     DAOQQV010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16553
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1087_length_16553_cov_8.096315"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2281
                     /locus_tag="PLL59_06930"
     CDS             <1..2281
                     /locus_tag="PLL59_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY23578.1"
                     /translation="KNGEVLIGANVYVQGNNALGATTNLYGFYSLSLPKGNYQIAFSY
                     LGYNTKIQDIVLEKDTRVNLELSDDSKTLDEVVIVDERKDENVKSTFMGKEELSTDRI
                     KSIPAFMGEVDIIKAIQLMPGVQAAGEGNSGIYVRGGGPDQNLVLLDDAIVYNTGHLF
                     GFFSVFNPDAVKNTTLYKGTMPANYGGRLSSVIDIQMKEGNNKRFEMEGGIGIISSRL
                     TLQGPFKKPKSEKTAKGSFLISGRRTYAFDIAQPFIKKTDFAGTNYYFYDLNIKANYQ
                     FSDKDRLYISAYFGRDVFVYNSQKNATKVKIPWGNATATVRYNHLFNDKIFMNMSFVF
                     NDYQFSFTGEQLDFGFKAKSGVRDYNAKVDFDYYINTKHKLKFGMNYTHHRMTPTQAS
                     ARSGDTDFESNQNKRYSSEFAWYIDHEFDVNEHLKFDFGFRLSMFQHIGPYKSITGDN
                     IIGYDTLTYKRSKPIKTYVGPEPRFSMRYAFGKNNSIKAGAGLNYQYIHLVSNSNSTL
                     PTDIWVQSSSMVKPQMALQYSLGYFKNFKDNMFEASIEVYFKHLWNQIDYSESYVQEL
                     NVDQELGYVYGKGKAYGAEFFLKKRSGKLNGWIGYTLSRSDRKFPDLNNGKVFPAKYD
                     RTHDLSINLNYDISKEWSVGATWVYATGNAITLPNELYFIEFGFVTGYGGRNTYRVPA
                     YHRMDFSLTYTPIPKRKPNRRWRSSYNISIYNVYNRKNTYFIYYKTEGSLVSGNLKFT
                     PINVSLFPVIPSFTWNFKF"
     gene            2284..3258
                     /locus_tag="PLL59_06935"
     CDS             2284..3258
                     /locus_tag="PLL59_06935"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HMY23579.1"
                     /translation="MKQKLQILFYVLTLFGVVNMLVSCEKEIKLKTSAPKNAIIVEGH
                     IENGMPPYVLLTKNSPFFGDININDLGAYFINGATITVITDNDSVQLVEYNSAFVQTL
                     PDSVVVAMAAQFGLQIDSAAQFPEISIYTVGLADADFVGQVGKQYNLRIELDGKIITS
                     TTTIPTPVFFDSLWILPHPNPVLADSFYQVYGRLNDPIELGNYYRYFTKADNEPFLIS
                     SQSVFDDAFFNGKKFQIFIPKGHPVGTMGSTDFNRAGYWDLNDSICTIKLSMIDKPHY
                     DFWRTLEANRQSNGNPFSSVVYVKSNIQGALGVWGGYGSITGSYVRVP"
     gene            complement(3264..3857)
                     /locus_tag="PLL59_06940"
     CDS             complement(3264..3857)
                     /locus_tag="PLL59_06940"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMY23580.1"
                     /translation="MFEFEKEYILENERALLRPLARNDKEHLLTFSLHEPELWQYSAQ
                     QADSEENLEKYVSTALEAREKQLEYPFIIFDKKTQEYAGSTRFYDIQNKHKSAQLGYT
                     WYGKKFQRTGLNRHCKLLMLSFAFDTLQFNRIEFRADLRNKKSITAMKAIGCKEEGVL
                     RQHLQLANNVCRDSIILSILREEWLYEVKENLLKKIY"
     gene            complement(3951..6170)
                     /locus_tag="PLL59_06945"
     CDS             complement(3951..6170)
                     /locus_tag="PLL59_06945"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HMY23581.1"
                     /translation="MNSKKVLLPVFLMAILSTANAQTTNVKSETKVAPVNKQKTSDKA
                     FTRPTLDKTTKGPQSVKPTKPYTISNDSLLKKMKPISYVKDPAIKLTDFSNKIKPQED
                     FFEYVNQRWIKTNPIPEDKASFGMFEKLEEKSKLAVKRILENAANVKIKVAKGSNTQI
                     LGDFYRNAMDTAAINKVGVQPLQTLFTEIENAKSTSALTKIFSKLGMRDINSPLGYYV
                     DADAKNTSRYIMYINQGGLGLPDKDFYFRKDEKSLKNLDAYREYVKNIFQLAGLDKTK
                     SSKEQANNVMEIEMLLAAASKSRVELRDPEKNYNLYTIEKLKQDFKHIDWDAFFAEMK
                     VAPKEIVLGQPSFYKTVDSMFAKVPHEKWVSYIEFQLVNGTAKHLNEELVNARFNFYG
                     KTLSGIQQLEPRWKRVAGISEQYLRDLIGQEYVKLNFSPKAKTRAHELVENIRAALKE
                     RINELTWMSSETKIKAQEKLAKIDVKVGYPDKWWTYENTDVSNQPHVLNVLNATYAEN
                     LRILARLSREKIDRTEWGMGPQTVNAYYNPLNNEIVFPAAILQPPFFYENADDAVNYG
                     GIGMVIGHEITHGFDDQGCQFDADGNLKNWWTDKDKKNYEELTSKFVKQYNGYHPFPN
                     DSLHVNGELTLGENIADLGGMIISYNALQKALAKKAQAPINSLTPDQRFFINYAIIWR
                     TSMRPEAMRMQVLSNEHSPAKFRVNGVLSNLPIFYKAFNVQATDKMYRKEEDRAKMW"
     gene            complement(6286..6852)
                     /locus_tag="PLL59_06950"
     CDS             complement(6286..6852)
                     /locus_tag="PLL59_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23582.1"
                     /translation="MKKKSFFWAITTSILITISCKKEPQAINEEELISTLIISLKKNS
                     SLSIQNYIWNDTDGIGGIDATVDSIIIDKDAEYNAYILLLNTHKTPTDTISNEVLAEG
                     TTHQFFYQSTPASALSNFQYQTPNDADGNPIGINFRFETIDASNTGFLKIILRHQPNK
                     LGSGVSSGDITNAQGETDIEIEFPFRIQ"
     gene            complement(6871..9195)
                     /locus_tag="PLL59_06955"
     CDS             complement(6871..9195)
                     /locus_tag="PLL59_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY23583.1"
                     /translation="MLQKLIAIYFLVFNALTYAQDCDIQIKGNILDEHDKSALEYATV
                     YIQELSFGVSADANAYFVLNNLCAGKYTFIVEHLGCKPDTIILTITKSITKNFYLEHH
                     AEELAEIITTASKLEKENTQTINIINQKVLNKLEGKDFASILSSVTGVNQLKTGTSIS
                     KPIINGLYGDRILIVNNGVKMETQNWGTEHATEIDPFASSTIKVIKGIGSLEYGTDAL
                     AGMVLIEPSILNKYKHITSSISLVGQTNGRSITTSAKIEQGFKKQIAYFLQGTYKRSG
                     DQQAPHYNLSNTGLQEGNFSSGFGVLKNSWDINIYYSLFQQQVGLLKSSHIGNLTDLQ
                     NAIESDTPLIVNRFTYNIENPKQKVAHHLAKINVVKYFKNQNHLAFTYSFQLNKRKEF
                     DIRRGGRSTIPSLHMNLVSNNFSAAYKRSKQFAKTNSRLEGKSGINLLMKINENKPET
                     GIRPLIPDYYQYSFGVFDLEKFIIENFIIEFGARYDFTQFQAYKFDRNNQLQKPVFKF
                     HTYAANIGGSWINNSNNIQVQSNISYNSRFPNTSELFSDGLHHGIATLEFGNDKLKPE
                     HGVKWINTVSINYRKFIKAEASFYISKIVDFIYLAPLPAPILTIRGAFPAFQFYQTDA
                     RLVGFDATLYSEPTSFLSLVFRSSIIRGKNTSANDNLVNMPSDRVSAAIDLHFDFKKI
                     KNVHFGMNVQHVLKQNKAPNLIRDYKPIPNAYTTLNTEIGFDYLASEKHKISFSVTGE
                     NITNANYRDYLNRFRYYADELGWNLIFRLKYIFS"
     gene            complement(9292..9366)
                     /locus_tag="PLL59_06960"
     tRNA            complement(9292..9366)
                     /locus_tag="PLL59_06960"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9329..9331),aa:Val,seq:tac)
     gene            9468..10634
                     /locus_tag="PLL59_06965"
     CDS             9468..10634
                     /locus_tag="PLL59_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMY23584.1"
                     /translation="MSYFQNRKRWKIILFIFAVSIVIFTLWYTHRLADYIAKEEIKKA
                     EEIASAYNLLNSNTSDELELSKALDKIKLNATIPVIWANSKDEILAAKNFDSLKINRN
                     KNYLAAELKKLKTKEQFIIVLLPDEQKQYLYYKDSDLLVSIRKYPFYVLALVALFFVI
                     SYAAFTSARRAEQNQVWVGLAKETAHQLGTPLSSLTAWIDILREKLNSDDDALMFDEM
                     QKDIDRLVLIAERFSKIGSVPELEKCRIIDVLQNGVDYISKRASKRINIYLEKDTNIN
                     LFAMLNPPLFEWVLENLMKNALDAMDDKGEIHVKAFEHDGFIFIDINDTGKGVPKNKF
                     EQIFEPGYSTKKRGWGLGLTLARRIVEEYHHGKIFVKESSPKGTTFRIQLQQAL"
     gene            10706..11830
                     /locus_tag="PLL59_06970"
     CDS             10706..11830
                     /locus_tag="PLL59_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMY23585.1"
                     /translation="MKVYFDNAATTPLDEEVLAAMMPYLKEHFGNPSTQYSYGRDTRA
                     AIEEARKEIAQLINAQASEVIFTSGATESNNMVIKGAVNYLGVERIITSPIEHHCVEY
                     SVVFCRDHLQVETAFVKIDDKGNIDFDDLETLLQSSSKKTLVSLMHANNEIGTLLDAE
                     RVGALCKKYNALFHSDTVQTFAHFPIDVQKMNVDFISGAAHKFHGPKGIGFLYMRKQH
                     KLQAYIHGGGQERGFRAGTENVYGIVGMAAAAKKAYANLENDKAYILGLKNYFIQQLQ
                     ANFQDIDFNGNIDENSLFTVLNVSFPPSDKAALLLFMLDIDGICCSGGSACGSGASAG
                     SHVISALAKDENRVSIRFSFSKHNTKEEVDYVISKIKEKL"
     gene            complement(11839..12333)
                     /locus_tag="PLL59_06975"
     CDS             complement(11839..12333)
                     /locus_tag="PLL59_06975"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HMY23586.1"
                     /translation="MQIRLFITGGTFDKEYNELDGTLFFEDTHVPEMLELGRCKLDVN
                     IRTLMMIDSLQMSDDDRVIIAHNCLQCDEEKIVITHGTDTMVETAKVLAEKINNKTIV
                     LTGAMVPYKFGSSDGLFNLGAALAYVQTLPNGVYIAMNGQIFNWNNVRKNKALGLFEP
                     LQES"
     gene            complement(12339..12917)
                     /locus_tag="PLL59_06980"
     CDS             complement(12339..12917)
                     /locus_tag="PLL59_06980"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMY23587.1"
                     /translation="MSSSTYICEQCGSASYEQFSRTQVRCLQCSTVSEFNTGYTPMPD
                     FQLSVDVDLLNIENKKTTPTSIAKRIINYFIDLLIISFIYAFYSSFGEQAKTEEITTK
                     IPDSQLLILLLIYGAYYIFMEYKFGKTIGKMITKTKVISIDNSALSLLQCIGRFLCRI
                     IPFEAVSGLFMNGNFWHDSIPRTMVVDDNNIN"
     gene            complement(12928..13929)
                     /locus_tag="PLL59_06985"
     CDS             complement(12928..13929)
                     /locus_tag="PLL59_06985"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HMY23588.1"
                     /translation="MNFQKQIAAFVALGDYILSNPTDLQEITHKSYLHNTWFTKENTE
                     KALQNIASAFLQKGKLENWLYTYAENLDKHTPKTFAIVAAGNIPLVAFHDVLCVLISG
                     NQLQIKLSDKDKFILPFLFDKLVEFEPTFEGKISMPFKLENFDAIIATGSNNSAKYFE
                     HYFSKYKNIIRKNRNSVAILSGHESIQDIENLGHDIFDYFGLGCRNVSKIFVPESYDL
                     LQLKEGFEKHKDIMQHTQYMNNLDYQRTVYLMNQIPLVDIDFINIVENPSMHSPISCL
                     HVERYKVIDMVNNSIIENKDELQCVVGKNYLSFGSSQQPALNDYADNVDTLAFILSN"
     gene            14002..14349
                     /locus_tag="PLL59_06990"
     CDS             14002..14349
                     /locus_tag="PLL59_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMY23589.1"
                     /translation="MAIRITDECINCGACEPECPNNAIYEGGIEWAMSDGTSLKGDYI
                     LMDGSVVDAEFKHKPVSNDVYFIVTDKCTECKGFHEEPQCAAVCPVDCCILDDDHVET
                     EEELLAKKAKLHL"
     gene            complement(14353..15525)
                     /locus_tag="PLL59_06995"
     CDS             complement(14353..15525)
                     /locus_tag="PLL59_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HMY23590.1"
                     /translation="MKKVFLFLFCFFSISINMIANNTVVGAERIDQYLPFLKGKKVGL
                     VVNQTSSVGNTHLLDTLLQLQINVSCVFAPEHGFRGTADAGEHVKNGIDPKTKTTIIS
                     LYGDNKKPKATQLKNIDVVLFDIQDVGVRFYTYISTLQYVMEACAENKKALIILDRPN
                     PNGHYVDGPVLDTINRSFVGMQPIPIVHGMTIGEYANMLNGEKWLANKLHCNLTVIKC
                     INYDHNTFYNLPIKPSPNLPNMAAIYLYPSLCFFEGTDHVSLGRGTNKPFQIYGSNVF
                     TGKLPYSFTPKPVEGAKNPPLLDKICYGYDLSKLSLDSLRKQKFTLKYVLKAYQMTPD
                     SIPFFNNFFVKLSGTDVLTTQIKSGLNEKVIKASWKEKISQFKAIRKKYLLYADFE"
     gene            15710..16447
                     /locus_tag="PLL59_07000"
     CDS             15710..16447
                     /locus_tag="PLL59_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY23591.1"
                     /translation="MKNILQKLGLVVFVILSVYNSNAQTAEAYLQAASAKHKIGDYAD
                     ALQDVNAAILLSPTYDAYLLRASIFYLTSNYSDAIRNASSALSLNENGYEAYLIKGKS
                     WYAIGDYESAMKDIEKTLAINTEQAEALYYEGLCKKIEGNYESAIDFYNRAIALNSND
                     AGMYNARAICFYFNGNTEKAFEDFDKAISIQPAFAEAYFNRAKFYYYEGETEKACTDW
                     LKAIALQYVEAEAYVKKKCAEQEISDK"
BASE COUNT         5222 a         2643 c         2870 g         5818 t
ORIGIN      
        1 aaaaaatgga gaagtactaa ttggtgcaaa tgtgtatgta caaggcaata atgcattagg
       61 agcaacgaca aatctttacg gattttattc tttgtctttg ccaaaaggaa attatcaaat
      121 tgctttttct tatttaggat acaatactaa aatccaagat attgttttgg agaaagatac
      181 acgtgtcaat cttgaattgt ctgacgattc aaaaacatta gatgaagttg tcattgttga
      241 tgaacgtaaa gatgagaatg tgaagagtac cttcatggga aaagaagaac tttctacaga
      301 tagaattaaa tcaattcctg cttttatggg cgaagttgat atcatcaaag ctattcaatt
      361 aatgccaggt gtgcaagctg ctggtgaagg aaattcaggc atttacgtgc gcggaggtgg
      421 ccctgaccag aacttagtat tactcgatga tgccatcgtt tataacacag gtcatttgtt
      481 tggcttcttc tctgtattta atcctgatgc agtaaaaaat accactttat acaaaggtac
      541 aatgccagca aactatggtg gtcgtctttc ttctgtaatt gatattcaaa tgaaagaagg
      601 caataacaaa cgattcgaaa tggaaggcgg tatcggtatt atttcttctc gactaacttt
      661 gcaagggcct tttaaaaaac ctaaatcaga aaaaacggcc aaaggctcat ttttaatttc
      721 tggacgacgt acttatgcat tcgatattgc gcagccgttt ataaaaaaaa cagactttgc
      781 tggcactaat tattacttct atgatttgaa tattaaagca aattatcaat tttctgataa
      841 agatagattg tatatcagtg cttattttgg tcgtgatgtt tttgtttata attcgcaaaa
      901 gaatgccact aaggttaaaa ttccatgggg aaatgcaact gctacagtgc gttacaacca
      961 tctatttaac gacaaaatat ttatgaatat gtcgtttgta ttcaatgact atcaatttag
     1021 ttttacaggc gaacaattag attttggatt taaagcaaaa tcaggcgttc gcgattataa
     1081 tgcaaaagtt gattttgatt attacatcaa taccaaacac aaattaaaat ttggtatgaa
     1141 ttatacacat catagaatga caccaacgca agcgtctgca cgtagtggtg ataccgattt
     1201 tgaatccaat caaaataaac gttattcaag cgaatttgct tggtatatcg atcatgagtt
     1261 tgatgtcaac gaacatttaa aatttgattt tggatttaga ttgagtatgt ttcaacacat
     1321 aggaccatat aaaagcatta caggtgataa tattattggc tacgatacgc ttacttacaa
     1381 acgcagcaaa cctataaaaa cctatgttgg tccagaacct cgctttagca tgcgatacgc
     1441 attcggaaaa aacaattcca taaaagcagg tgctggttta aactatcaat acattcattt
     1501 agtgtctaac tcaaatagca cattgccaac tgatatttgg gtgcaaagtt cttccatggt
     1561 aaaaccacaa atggcattgc aatactcact aggttacttc aaaaatttta aagataatat
     1621 gtttgaagct tcaatagaag tgtattttaa acatctctgg aatcaaattg attatagcga
     1681 atcgtatgtg caagaattaa atgtcgatca agaattaggg tatgtttatg gtaaaggtaa
     1741 agcgtatggc gccgaatttt ttctaaaaaa acgaagtgga aaattaaatg gctggattgg
     1801 ctatactttg tcgcgtagtg atagaaaatt tcctgacttg aataatggaa aagtatttcc
     1861 ggcaaagtat gacagaacac atgatttgtc tattaatctt aattatgaca ttagcaaaga
     1921 atggagcgta ggcgcaactt gggtttatgc aacaggtaat gcaattactt tgcctaatga
     1981 attgtatttt attgagtttg gttttgtaac aggctatggc ggtagaaata cctatcgtgt
     2041 gcctgcatat catcgtatgg atttttcatt gacatacacg cctataccaa agcgcaagcc
     2101 caataggaga tggcgttcca gttataatat ttcaatctac aatgtgtaca atagaaagaa
     2161 tacatacttc atttactata aaacggaagg aagtttagtg agcggtaatt tgaaatttac
     2221 acctattaat gtttcacttt ttccagtaat accatcgttc acttggaatt ttaaatttta
     2281 gacatgaaac aaaaactaca aatattattt tatgtgctga ctttgtttgg agtagtcaat
     2341 atgttggttt catgcgagaa agaaattaaa ttaaaaacaa gtgctcctaa aaatgcaata
     2401 attgtagaag gtcatattga aaatggcatg ccaccatatg tattgcttac aaaaaattca
     2461 cctttcttcg gcgatatcaa tatcaatgac cttggtgctt attttattaa tggtgcaaca
     2521 ataacagtaa ttacagataa tgattccgtg caattagtag aatataattc tgcattcgta
     2581 caaacattac cagattcagt agtggtggcg atggcagcac aatttggatt gcaaattgat
     2641 agtgctgcac aatttccaga aataagtatt tatactgttg gtcttgcaga tgccgatttt
     2701 gttgggcaag taggcaaaca atacaattta cgtatcgaat tagatggcaa aataattact
     2761 tccacaacaa ctattcctac gcctgttttt tttgattcac tttggatttt accacatccc
     2821 aatcctgttt tggcagattc attttatcaa gtgtatggaa gattgaatga cccaatcgaa
     2881 ttaggaaatt attatcgcta ttttactaaa gcggacaatg aaccattttt aattagtagc
     2941 caatccgtat ttgacgatgc attttttaac ggcaaaaaat ttcaaatatt tatacctaaa
     3001 gggcacccag ttggcacaat gggaagcaca gattttaatc gggcaggata ctgggatttg
     3061 aatgactcta tttgcacgat taaactttcg atgatagata aaccacatta tgatttttgg
     3121 cgcacattag aagctaaccg tcaaagcaat ggcaatccat ttagctctgt cgtttatgtg
     3181 aagtctaaca tacaaggtgc attaggtgtt tggggcggct acggcagtat taccggtagt
     3241 tatgtaagag tgccttaaat tgtttaataa atttttttca atagattttc ttttacttca
     3301 taaagccatt cttctcgcaa gatacttaag atgatgctgt cgcgacatac attatttgct
     3361 agttgtaaat gctgacgcaa aacgccttct tctttacagc caattgcttt catcgctgta
     3421 atgctttttt tatttctcaa gtcggctcta aattcaatac gattaaattg caaggtgtcg
     3481 aaggcaaagc taagcattaa taatttgcag tgtctgttta agccagttct ttgaaatttt
     3541 tttccatacc aagtatagcc aagctgtgcc gatttatgct tgttttgtat gtcgtaaaaa
     3601 cgcgtgctgc ctgcatattc ttgcgttttt ttatcaaaaa taataaacgg atattccaac
     3661 tgtttttctc tggcctctaa tgcggtgctt acatattttt ctaagttttc ttcgctgtct
     3721 gcttgctgag cagagtattg ccaaagttca ggctcgtgta acgaaaaggt aagtaaatgt
     3781 tctttgtcat tcctcgccaa tggacgcagt aatgctctct cgttttctaa aatatattct
     3841 ttctcaaatt caaacatata ttgttgtttt aaaaaattaa aattacacat ttttcaaaac
     3901 aaaaaatgcc accgaatatg ggtggcattt ttttataact ataaaattga ttaccacatt
     3961 tttgctctgt cttcttcctt tctatacatc ttatctgtag cttgtacatt aaatgctttg
     4021 tagaaaatcg gtaagttaga caatacgcca tttactctaa atttagcagg tgagtgctcg
     4081 tttgaaagta cctgcatacg catggcttct ggtcgcatac ttgttctcca aatgatagca
     4141 taattgataa agaaacgttg gtcaggtgtt aagctgttta ttggtgcttg cgcttttttt
     4201 gctaatgctt tttgtaaggc attgtagcta ataatcatac cacctaagtc ggcaatgttt
     4261 tcgcctagtg tcaattcacc atttacgtgt aagctgtcat taggaaaagg atggtagcca
     4321 ttgtattgtt ttacaaattt cgaagttaac tcttcgtagt tttttttgtc tttatctgtc
     4381 caccagtttt ttaaattacc gtcagcatca aactggcagc cctggtcatc aaaaccatgt
     4441 gtaatttcat gaccaatcac catgccaatt ccaccataat tcactgcatc atctgcgttt
     4501 tcgtaaaaga aaggaggctg taatatagct gctggaaaaa cgatttcgtt gtttaaagga
     4561 ttgtaataag catttacggt ttgtgggccc atgccccatt ctgtacggtc tattttttcg
     4621 cgacttaaac gagctaatat tcttaagttt tcagcgtatg ttgcatttaa gacatttaaa
     4681 acatgtggtt gattgctaac atctgtattt tcgtatgtcc accatttatc aggatagcca
     4741 acttttacat ctatttttgc taatttttct tgtgctttta tttttgtttc actgctcatc
     4801 caagtgagtt cattgatgcg ttcttttaaa gcagctctaa tgttttcaac taattcatgc
     4861 gctcgagttt ttgctttagg agagaagttt aattttacat attcttgtcc aattaaatct
     4921 cttaagtatt gctcagaaat acctgctaca cgtttccatc ttggttctag ttgttgaatg
     4981 ccacttaatg ttttgccata aaaattaaat cttgcattga ctaattcttc attcaaatgt
     5041 tttgctgttc catttactaa ttgaaactcg atataagata cccatttttc gtgtggcact
     5101 tttgcgaaca tgctgtcaac tgttttgtaa aatgatggtt gtcctaatac aatttcttta
     5161 ggtgctactt tcatttcagc aaagaatgca tcccaatcga tgtgtttaaa atcttgtttt
     5221 aatttttcga tggtatatag gttgtaattt ttttcaggat ctctgagttc tactcttgat
     5281 tttgaagcag cagccaataa catttctatt tccattacat tatttgcttg ctcttttgaa
     5341 gatttagttt tgtcaagacc tgctaattga aaaatatttt taacatattc gcgatatgca
     5401 tctaaatttt tcaatgactt ttcatctttt ctgaaataaa aatctttatc aggcaaccct
     5461 aatccacctt ggttgatata cataatataa cgagatgtgt tttttgcatc agcatcaaca
     5521 taataaccaa gaggagagtt gatgtcgcgc atgcctagct ttgaaaatat ttttgttaat
     5581 gcgctagtcg attttgcgtt ttcaatttca gtaaacaaag tttgcaaagg ctgaactcca
     5641 actttattga ttgcagctgt gtccattgca tttctataaa agtcgcctaa aatttgtgtg
     5701 tttgagcctt tagcaacctt aatttttaca ttggcagcat tttctagtat tcgttttaca
     5761 gctagtttgc ttttttcttc taatttttca aacatgccaa agcttgcttt gtcttccgga
     5821 attggatttg tttttatcca gcgttggttg acatattcaa agaaatcttc ttgtggtttt
     5881 attttattgc tgaaatcagt cagtttgata gctggatctt taacataaga aatgggtttc
     5941 attttcttta gtaaggaatc attgctaata gtgtatggct ttgtcggttt tacagactga
     6001 ggtccttttg ttgttttatc cagcgtaggt ctggtaaatg ctttgtccga tgttttttgt
     6061 ttattgaccg gcgctacttt tgtttctgat ttaacatttg ttgtttgcgc attcgctgta
     6121 cttagtattg ccataagaaa aacaggcaat aatacttttt tactattcat actattttgt
     6181 tatgttgtaa aattagtgtt tagaatgtat taaaaattac aaaagagcgt taaaaaatgt
     6241 aattgtgaga aaaaaataag ttggtattta gatttagtgt gataattact gaatacgaaa
     6301 gggaaattct atttcgatat ctgtttcgcc ctgtgcattt gtgatgtcac ctgatgaaac
     6361 gccacttcct aatttattgg gttgatgtcg taatattatt tttaaaaagc cagtattaga
     6421 tgcatctata gtttcaaatc taaaatttat accaatagga ttgccatcag cgtcatttgg
     6481 tgtctgatat tgaaaattgc ttaaagcgct tgctggcgtt gattgataaa agaattgatg
     6541 tgtagtgcct tctgccaaaa cttcattaga aattgtgtct gtcggtgttt tatgtgtatt
     6601 taataataga atgtaagcat tgtattcggc atctttatct ataatgatac tatctactgt
     6661 cgcatctata ccacctatac catcagtatc attccagata tagttttgta tggataaaga
     6721 actattcttt tttaaagaaa taatcaacgt gcttattaat tcttcttcgt taattgcttg
     6781 tggctccttt ttgcagctaa ttgtaatcaa aatacttgtt gtgattgccc aaaaaaatga
     6841 tttttttttc ataataatta atttatagag ttaactaaaa atgtatttca atctgaatat
     6901 taaattccaa cctaactcat ctgcgtaata tctaaaacgg tttagataat ctcgataatt
     6961 ggcatttgta atgttttcac ctgtgactga aaatgaaatt ttatgtttct ccgaagcaag
     7021 ataatcaaaa cctatttctg tatttagtgt tgtgtatgca tttggtatgg gcttgtaatc
     7081 acgaataaga ttaggtgctt tattttgttt taaaacatgt tgaacattca taccgaaatg
     7141 aacatttttt atttttttaa aatcaaaatg taaatcaatt gcagcactga ctctgtcaga
     7201 aggcatgtta actaaattat catttgcact cgtatttttg cctcttataa ttgaagaacg
     7261 aaatactaaa gataggaatg aagtaggctc ggaatataat gttgcatcaa accctacgag
     7321 gcgcgcatcg gtttgataaa attgaaatgc tggaaacgcg ccacgtatag ttaatattgg
     7381 tgcaggcagt ggtgcaagat agatgaagtc aactatttta gatatgtaaa agctagcttc
     7441 tgcttttata aattttctgt aatttatgct tactgtgttt atccatttta caccatgttc
     7501 aggctttagt ttgtcatttc caaattctaa tgtagcaatg ccgtggtgta aaccatcgct
     7561 aaataattcg ctggtatttg ggaagcgtga attgtaagag atatttgatt gtacttgaat
     7621 attattagaa ttatttatcc aacttccgcc aatatttgca gcatatgtgt gaaatttaaa
     7681 cactggtttt tgtagctgat tatttctgtc aaatttataa gcttgaaatt gtgtgaaatc
     7741 atatctagca ccaaattcaa tgatgaaatt ttcaatgatg aatttctcca aatcaaaaac
     7801 accaaagctg tactgataat aatcaggtat caagggccga ataccagttt caggtttgtt
     7861 ctcatttatt ttcattaata aatttatacc tgattttcct tctaaacgac tatttgtttt
     7921 tgcaaattgt ttagatcgtt tatatgctgc cgaaaaatta tttgaaacaa gattcatgtg
     7981 taacgaagga atagtagaac gaccaccacg acgaatatca aactcttttc tcttgtttaa
     8041 ttgaaatgaa taagtaaaag ccaaatgatt ttgattttta aaatatttta caacatttat
     8101 ttttgctaaa tgatgcgcaa ctttttgttt tggattttcg atattgtatg taaatctatt
     8161 tactattaaa ggtgtgtcac tttctatggc attctgtaga tccgttaaat ttccaatgtg
     8221 cgaactcttt aataaaccta cttgttgttg aaataatgaa taataaatat tgatgtccca
     8281 gctatttttt aacacaccaa agcctgaaga aaaatttcct tcttgtaaac ctgtattaga
     8341 taaattataa tgcggtgctt gctggtcgcc tgaacgttta taggtgcctt gcaaaaaata
     8401 ggctatttgt tttttgaaac cttgttcaat ttttgcggaa gtagtaatgc ttcgtccatt
     8461 agtttgtcca actaaactaa ttgatgaagt aatatgtttg tatttattca agatagatgg
     8521 ctctattaac accattccag ccaatgcatc agtaccgtat tctaggcttc caataccttt
     8581 tattacttta atagtagagg aagcaaaggg atcaatttct gttgcatgtt cagtgcccca
     8641 attttgcgtt tccattttta caccattatt tactattaaa attctatcgc catataaacc
     8701 atttataatt ggcttgctaa tgcttgttcc tgtttttagt tgatttacgc ctgttactga
     8761 ggataaaatg ctagcaaaat cttttccctc tagcttattt aatacttttt gattgataat
     8821 attgattgtt tgtgtatttt ctttttctaa tttagatgcg gttgttatta tttctgctaa
     8881 ttcttcagca tgatgttcta agtaaaaatt ttttgtgatt gattttgtta ttgtaagaat
     8941 aatagtatct ggtttgcatc caagatgttc tacaataaat gtatattttc cagcacataa
     9001 attatttaat acaaaatatg cattagcatc tgcgctgacg ccaaaactta attcttgaat
     9061 atatacagtt gcatattcta atgcagattt atcgtgttca tctaaaatat ttccttttat
     9121 ttgaatatca caatcttgag catatgttag tgcattgaaa actaagaaat aaatagcaat
     9181 gagtttttgc aacataattg caaatatagt aagaatattc aaatgcaact atgttgcaag
     9241 tatattttta ttaaaaaaaa taccattcag ttattgaatg gtatttcgaa gtgggcgcac
     9301 acgggttcga accgcggacc ccctgcttgt aaggcaggtg ctctgaacca gctgagctat
     9361 gcgccctctt ttttaaagag agtgcaaaga tataaaatag tttttcataa acaagcaagt
     9421 attaaaaaaa aatgtgtaat ctttacatta acactatttt tatttcaatg tcatactttc
     9481 aaaataggaa aagatggaaa attatactgt tcatatttgc agtgagtatt gtcattttta
     9541 cactttggta cacgcatcgc ttagcggatt acatcgccaa agaggaaata aaaaaagcag
     9601 aagaaattgc aagtgcttat aatttattga atagcaatac ttctgatgag cttgagcttt
     9661 caaaggcatt agataaaatt aaattgaatg ccaccattcc tgtgatttgg gcaaatagta
     9721 aagatgaaat attggctgca aaaaattttg attccctaaa aattaataga aacaagaatt
     9781 acttagcagc tgaattaaaa aaactcaaaa cgaaagaaca atttattata gtgttgcttc
     9841 cagatgagca aaaacaatat ctgtattata aagattccga tttgttggtg agcatacgta
     9901 aatatccttt ttatgtgctg gctttagtcg cgttgttctt tgtcatcagc tatgcagcct
     9961 ttacctcggc acgaagagca gagcaaaatc aagtttgggt tggattggca aaagaaacag
    10021 cacatcagtt aggcacacca ctttcgtcat taacggcatg gattgatatt ttaagagaaa
    10081 aactaaacag cgacgatgat gcgcttatgt ttgatgaaat gcaaaaagat atcgatagat
    10141 tggtgttgat tgcagaacgc ttttcgaaaa taggttcggt tccagagtta gaaaaatgta
    10201 gaattattga tgtgttacaa aatggcgtag attatatttc gaaacgagca tcgaaacgca
    10261 tcaatattta cttggaaaaa gataccaata tcaatttgtt cgcgatgtta aatccaccat
    10321 tatttgaatg ggttttagaa aatttaatga aaaacgcgct tgatgctatg gacgacaagg
    10381 gcgaaattca tgtcaaagcg tttgaacacg atggctttat ttttattgat atcaacgata
    10441 caggaaaagg agtgccaaaa aataaatttg aacaaatttt tgagcctgga tatagcacaa
    10501 aaaaacgagg ttggggtctt ggattaacac tggctcgacg tatcgtggaa gaatatcatc
    10561 acggtaaaat atttgttaaa gaatcatcac caaaaggaac tacatttcgt attcaacttc
    10621 aacaggcatt gtaaagttta tgttgcttgt tataacaact aaaattgttt tgtaactttg
    10681 catatcatta atttatcctt ttcacatgaa agtttatttc gataacgctg caacgacacc
    10741 attagatgaa gaagttttag cagcgatgat gccttattta aaagagcatt ttggaaatcc
    10801 atctactcaa tattcttatg gccgcgatac acgtgccgcg attgaagaag cgagaaagga
    10861 aattgcacaa cttatcaatg cgcaagcaag cgaagtgatt tttacttcag gtgctacaga
    10921 gtcgaataat atggttatta aaggtgcggt aaattattta ggagttgagc gcatcatcac
    10981 ttctccaatc gaacatcatt gtgttgaata ttctgttgtt ttttgcagag atcacctaca
    11041 agtagaaact gcttttgtaa agattgatga taaaggaaat attgattttg atgatttaga
    11101 aacattgctt caatcatcat ctaaaaaaac attagtatcg ctaatgcatg caaataacga
    11161 aattggaacc ttgctcgatg cagaacgtgt tggtgcacta tgcaaaaaat ataatgcatt
    11221 atttcattca gatacagtgc aaacattcgc tcattttcct attgatgttc aaaagatgaa
    11281 tgtagatttt atttcaggcg ctgcacataa atttcacgga cctaaaggaa tcggattttt
    11341 gtatatgcgt aaacaacaca aattgcaagc ttatattcat ggcggcggac aggagcgtgg
    11401 ttttcgtgct ggtacagaaa acgtttatgg tattgtgggc atggctgccg cggctaaaaa
    11461 ggcatatgct aacctagaaa atgataaagc ttatatttta ggtttgaaaa attattttat
    11521 acaacaattg caagcaaatt ttcaagacat tgattttaat ggaaatatag atgaaaatag
    11581 cttatttact gtgctgaatg tatcttttcc accaagcgat aaggcagcat tgttgttgtt
    11641 tatgttagac attgatggta tttgttgtag tggtggcagc gcttgtggca gcggcgcgtc
    11701 tgctggttcg catgttatta gcgcacttgc taaagatgaa aatcgtgttt ctattcgatt
    11761 ttctttttct aaacacaata caaaagaaga agttgattat gtcatttcta aaataaaaga
    11821 gaagttataa gtcttttttt aagattcttg tagtggctca aacaagccta atgctttgtt
    11881 tttacgaaca ttattccaat taaaaatttg tccattcatg gcaatataaa cgccattggg
    11941 aagcgtttgt acataagcca atgctgctcc aaggttgaat aaaccatctg agcttccaaa
    12001 tttataagga accatagcgc cagtaagtac aattgtttta ttgtttattt tttctgctaa
    12061 tacttttgct gtctccacca tggtgtctgt gccatgtgtg atgacaattt tttcctcatc
    12121 gcattgcaag caattatgtg ctatgataac acgatcgtca tcactcattt gcaagctatc
    12181 aatcatcatt agtgttctaa tgtttacgtc taatttacat cttcctaatt ccaacatttc
    12241 tggcacgtgt gtgtcttcaa aaaataaagt accatcgagc tcgttatatt ctttgtcgaa
    12301 tgtgccacct gttataaata atcgaatttg cattggaatt aatttatatt attgtcatca
    12361 acaaccattg tgcgtggaat tgaatcatgc caaaaattgc cattcataaa taatccactt
    12421 accgcttcaa atggaattat tctacacaaa aatctgccta tgcattgcag taagcttaat
    12481 gctgaattat caattgaaat aacttttgtt tttgttatca ttttaccaat tgttttcccg
    12541 aatttatatt ccataaatat atagtaagct ccataaataa gcaatagtat taatagctgg
    12601 ctgtcaggaa tttttgttgt aatttcttcc gtttttgctt gctcaccaaa gctactataa
    12661 aaagcatata tgaaactaat aattagcaaa tcgatgaaat aattaatgat tctttttgca
    12721 attgaagttg gcgttgtttt tttattttcg atattgagta agtcaacatc tacactcagt
    12781 tggaagtctg gcattggcgt atagccagta ttaaattcac ttactgtgct acattgtaag
    12841 caacgtactt gtgtacgcga aaattgctca taacttgcac tgccacattg ttcacagata
    12901 tatgttgaac tactcatttc tcttttttta gtttgataaa ataaatgcta aagtatccac
    12961 attgtcagca taatcattca atgctggttg ctggctgctg ccaaatgaaa gatagttttt
    13021 tcctacaaca cattgcaatt cgtctttgtt ttcgataata ctattgttca ccatgtctat
    13081 gactttataa cgctccacat gtaagcacga aataggagag tgcatgctcg gattttctac
    13141 tatattgata aaatcgatat cgaccaatgg tatttgattc atcaaatata cagtgcgttg
    13201 ataatctaaa ttattcatgt attgtgtgtg ctgcatgatg tctttgtgtt tttcaaagcc
    13261 ttcttttagt tgtaataaat cgtaagattc aggaacaaaa attttagata catttcgaca
    13321 gcctaatcca aaataatcga aaatatcatg tcctaaattt tcaatatctt gtatgctttc
    13381 atgtccactt aaaatggcaa cagaatttct gtttttacga attatatttt tgtatttact
    13441 aaagtaatgc tcaaaatatt ttgcagaatt gttgctacca gttgcgatga tggcatcaaa
    13501 attttctagc ttaaaaggca tgcttatttt accttcaaag gtgggctcaa attcaactaa
    13561 tttgtcaaat aaaaatggca atataaattt gtctttatcg gataatttta tttgtaattg
    13621 attgccactt attagtacgc acaacacatc atgaaaagca actaatggta tatttcctgc
    13681 tgccactatc gcaaatgttt tcggtgtgtg cttgtctaaa ttttcagcat acgtatataa
    13741 ccaattttct aatttccctt tttgtaaaaa tgctgatgca atattttgca atgctttttc
    13801 tgtgttttct ttggtaaacc aagtgttatg tagatagctt ttgtgtgtaa tttcttgtaa
    13861 atcagttgga ttactaagaa tgtaatcacc taaggcaaca aaggcagcta tttgtttttg
    13921 gaaattcatt aacacaaaat tattaattat atgctaccga aaatggtatc tatgtttatt
    13981 tttgtatcaa acttacataa aatggctata cgtataactg acgaatgtat taattgtggt
    14041 gcttgcgaac cggaatgtcc gaataatgca atctatgaag gtggcattga gtgggcaatg
    14101 agtgatggaa cttctttaaa aggcgattat attttaatgg atggaagtgt tgttgatgca
    14161 gaattcaaac acaaacctgt gtctaatgat gtttatttta tagtaactga taaatgcaca
    14221 gaatgtaaag gtttccatga agagccacaa tgtgctgcgg tttgccctgt tgattgttgt
    14281 attttagatg atgaccatgt agaaacggaa gaagagttat tagctaaaaa agcaaaactt
    14341 catttatagt ctttactcga aatcagcata aagcaaatac ttcttacgta ttgccttaaa
    14401 ttgactaatt ttttctttcc aagatgcttt tatgactttt tcatttaagc cagatttaat
    14461 ttgtgttgtt aaaacatcgg tgccacttag ctttacaaaa aaattgttga aaaatggaat
    14521 gctatcaggt gtcatctgat aggcttttaa gacatacttt aatgtaaatt tttgcttacg
    14581 caaactatct aaagacaatt tgcttaaatc atatccgtag catatcttat ctaataatgg
    14641 cggatttttt gcgccttcta ctggttttgg tgtgaaggaa tagggcaatt ttcctgtaaa
    14701 aacattgctg ccataaatct gaaaaggctt attcgttcct cttcctaagc tgacatgatc
    14761 tgttccttca aaaaaacata aactcggata taagtagatt gctgccatat tcggtaagtt
    14821 tggcgaaggt tttatcggca agttgtaaaa tgtattatgg tcgtagttaa tacatttaat
    14881 tacagtcaaa ttgcagtgta atttattggc taaccatttt tcgccgttta acatattcgc
    14941 atactcgcca atggtcatac catgcacaat tggaataggc tgcatgccaa caaaacttct
    15001 atttattgta tctaaaactg gaccatcaac ataatgtcca ttcggattcg gtctgtccag
    15061 gataatgagt gccttcttgt tctctgcaca tgcttccatt acgtattgta aggtagaaat
    15121 ataggtgtaa aaacgaacgc caacatcttg tatgtcaaac aggacaacat ctatattttt
    15181 tagttgtgtt gctttcggtt ttttattgtc tccatatagc gaaataatcg tagtcttcgt
    15241 tttgggatca atgccattct tcacatgttc gcctgcatcg gcagtgcctc taaaaccatg
    15301 ttcaggtgca aaaacacagg atacatttat ttgtaactgt aataatgtgt caagtaaatg
    15361 cgtattgcca acacttgatg tttggttgac gactaatcca acttttttcc cttttaaaaa
    15421 tggtaaatat tggtctattc tctcagcacc tacaacggtg ttatttgcaa tcatattaat
    15481 agatatcgag aaaaaacaaa ataagaagag gaatactttt ttcatgttgc ctgtttttac
    15541 cttttatagg tttgcaaaat aaaacaaatt aaatgccaag acaacagtac ttttaaataa
    15601 tgtacatcat tttgaaatta ttatttatta tttctattta attagaaatt ttacagttat
    15661 tagttatact gcaatttttt attaatttta gctgtcaata ctattttata tgaaaaatat
    15721 tttacaaaaa ttaggcttag tagtgtttgt tatactgtct gtatataaca gtaatgcaca
    15781 aaccgctgaa gcttatttac aagcggcatc tgcaaagcac aaaattggcg attacgctga
    15841 tgcattacaa gatgtaaacg cagctatatt attgagtcca acttatgatg cttatctttt
    15901 gcgtgcttca atattttatc tgacttctaa ttattctgac gcgatacgca atgcaagctc
    15961 ggcactttca ttaaatgaaa acggatatga agcatatcta atcaaaggta aatcgtggta
    16021 tgcaattgga gattatgaat cagccatgaa agatattgaa aaaacattag caatcaatac
    16081 agaacaagcg gaagcgcttt attacgaagg cttatgtaaa aaaattgagg gtaactatga
    16141 gagcgcaatc gacttttata atcgagctat tgcattaaat agtaatgatg caggtatgta
    16201 taatgcacgt gctatctgtt tttattttaa tggcaataca gaaaaagcat ttgaagactt
    16261 tgataaagca atcagcattc agccagcatt tgcagaagcg tattttaatc gtgctaaatt
    16321 ttattattat gaaggtgaaa cagaaaaagc atgcacagat tggttgaaag caatcgcttt
    16381 acaatatgta gaagcagaag cgtatgtcaa gaaaaaatgt gcagaacaag aaataagtga
    16441 taaataaatt taagggaaaa gagaataaaa aaaggttcag atttttctga accttttttt
    16501 tgtagcccgt aggggaatcg aacccctgtt tgaagaatga aaatctcctg tcc
//
LOCUS       DAOQQV010000054        16438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1101_length_16438_cov_7.759812, whole genome shotgun sequence.
ACCESSION   DAOQQV010000054 DAOQQV010000000
VERSION     DAOQQV010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16438
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1101_length_16438_cov_7.759812"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PLL59_07005"
     CDS             <1..1193
                     /locus_tag="PLL59_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMY23592.1"
                     /translation="KPILALDPGFATGCKLVCIDDKGTLLEDSVIYPHPPLNKKREAE
                     DIVRGLLYKYNINTIAIGNGTAGRETEDWLNKISWERKPRLFSVNESGASIYSASDVA
                     REEFPDKDITVRGAVSIGRRLMDPLAELVKIDPKSIGVGQYQHDVNQYLLKQSLDETV
                     VSCVSQVGVNINTASKHLLMYVAGLGQSTAENIIQHRTNNGIFKSRKELLQVSRLGEK
                     IFEQAAGFLRIADAQNPLDNSAVHPERYALVEQMAHDQNVTIQELLANKDVRKKIDLK
                     KYISEEVGLPTLQDILKELEKPGRDPRDEIQAFVFDATIKTIEDLHTGMVVPGIVTNI
                     TNFGCFVNIGVKQDGLIHISELANKFIKDPNEIVKLQQAIRVKIIEVDLSRKRIVLSK
                     KQAE"
     gene            1252..1776
                     /locus_tag="PLL59_07010"
     CDS             1252..1776
                     /locus_tag="PLL59_07010"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HMY23593.1"
                     /translation="MTFVAIDFETATSQPYSACAVGIISVQDSVITDEFECLIQPPNN
                     LYFQQNTKVHGLSANDTKHALAFDNVFVEIQQRLIGKQVVAHNESFDRNVLKQCIAHY
                     QLSSKGLLLHRNWNCTVKLSQHLGYSPNKLSDCCQRFNIPLQHHDALSDARACAILFM
                     QLSAQQRKNKMYLF"
     gene            complement(1789..2649)
                     /locus_tag="PLL59_07015"
     CDS             complement(1789..2649)
                     /locus_tag="PLL59_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C
                     domain-containing protein"
                     /protein_id="HMY23594.1"
                     /translation="MKRYAFCSINILIFSFAFIVACTKTKNDDKEDTLLKKHYDEVCF
                     LMTHNAMNNSEKSYAIPNQTHSITHQLQNGVRGLMIDTYDGAGGVAKTYHAIEAMGSQ
                     NLVDVLKEVNDFMVTNPKSVVSIIFQNEGSNAQLQKAIDSSGLSAMTYTHNTANWATL
                     QSMIDSNQRLVLFAEFSKLPSISYLHYAWSMIFDTKYSYQNRSQFDTDVNRGGSGTKE
                     LYLINHWLQNVLGLPDKNLAPQANARAVVGKRVQDCATDNSHFVNFLGVDFYEIGAAK
                     AIVDSINAVP"
     gene            2742..3584
                     /locus_tag="PLL59_07020"
     CDS             2742..3584
                     /locus_tag="PLL59_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMY23595.1"
                     /translation="MATQKKNIISKIFDDQRIQEYVNVFPNNVGSFGFDAWGFNIKNM
                     KNNMRFTKYLYEEFYKVEAFGLENIPKDGRCLIIPNHSGQLPFDGMLVGYAMITNEKG
                     PRAPKAMVERFLPTVPFIGNWLNSVGAVIGDPINCERMLEREEAIVVFPEGVRGSNKL
                     YKHKYQLQRFGAGFMHLAMKHDAPIIPVGIVGMEETIRSYADIKPLANLLGMPVAPLV
                     IPFIFPSKVYLLFGKPIYFKNDTYKESEINARVDIVKDAINELLKEGLAKREAYIQEK
                     KQKK"
     gene            3617..4549
                     /locus_tag="PLL59_07025"
     CDS             3617..4549
                     /locus_tag="PLL59_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY23596.1"
                     /translation="MEPRKKILVTGAAGALAKKVIDKLKKDYKIIAVDFRTKVDLGIP
                     VESYKVDFNKRLFEDIFREHQFDGIIHLGRISAQELNRFTRYNANVIGTKKLFDLARK
                     YNVPKTLVLSTYFVYGASAYNPSLLDETTPLKASELTMDLVDSVELENLSTIYLYKHP
                     ELNITVLRPCNIAGPGVRNTFSLLLSEKRSPVLWGFSPLMQFIHIDDMRDAICTAFEK
                     NFPGVYNVAPDDYIGFQDAIKAAGSSTIPIPSIPPSLTESISKFLKWKSFPPYLINYF
                     KYPVIIDGSLFAKTFNFRPKKTLSDIFTYYRNLK"
     gene            4573..5295
                     /locus_tag="PLL59_07030"
     CDS             4573..5295
                     /locus_tag="PLL59_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23597.1"
                     /translation="MFYKLDMRTLLLSLLLIACTIHLQAKENTFFAGIKLNNGRKIDA
                     IKNSFANGTFRDKSYFLSNTYYFGGTIKNVSISCGIAYNLYHYNDKRDYLFLIIGPNY
                     QKKNNHILSIPFNVDYNINIYKEKLFVIVGAGIEYTITLSQVLKPNNGTVFPDVKVKH
                     KALNNTYKTHALLFNFRSGLLYAVNKRVDIFTSFDFGIYANKYMALRNDYPSANKKYT
                     YLLSNSIGVAIKFAKLKSKSKN"
     gene            complement(5292..6638)
                     /gene="radA"
                     /locus_tag="PLL59_07035"
     CDS             complement(5292..6638)
                     /gene="radA"
                     /locus_tag="PLL59_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HMY23598.1"
                     /translation="MSKTKSIYICQNCGTNSPKWMGKCNNCGEWNSYVEELIEPKSKS
                     AAVVNAPKNKPQLLTEISSENRQRIITSDPELNRVLGGGIVPGSLILIGGEPGIGKST
                     LLLQVVLQTKGLKTLYVSGEESEQQLKMRADRIHQQNSDILVYTETSVESIVQQIEQT
                     QPQLLVIDSIQTLETSLIESAAGSVSQIREATHVLQQYAKRHHLPIFIVGHITKEGTI
                     AGPKILEHMVDTVLQFEGDRNYNYRILRTQKNRFGSTAELGIYEMRGDGMRAVSNPSE
                     LLITQRDEQVSGVAIAVTMEGMRALLVEVQALVSPAVYGTPQRSATGYDLRRLGMLLA
                     VLDKRCGFRFGAKDVFLNIAGGLRVEDPAVDLAVVAALLSSYEDIAINNKYSFSGEVG
                     LSGEIRAVNKVEQRIAEAGKLGYEKIIISKYNQSVKSESATIQLVKSVAEFYKLLF"
     gene            6702..7673
                     /locus_tag="PLL59_07040"
     CDS             6702..7673
                     /locus_tag="PLL59_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY23599.1"
                     /translation="MKRSLLYLFVAIVFTNCVTQKEYDKALNDYLNEAIKNKYLNESL
                     DSLKADCTTKNTQLSTQIEQLRADSSELATLIESMKKEIDEQALEYAKQQQEYLKKLK
                     DAGDKNQKTNADLIQMQLDLEKQRIALQQKETDLKKANTDLLAREAKINELEKLLTEQ
                     KTKADALRDAIKKALTDFSAGELSVYTKDGKVYVSMSDKLLFKSGSTTVESKGVDALG
                     KLSDVIKKNPDILVNIEGHTDNVPYGTGSGLIKDNWDLSLMRSSSVLHILTDKYKVSS
                     LQVIASGRGEFSPKESNSTADGRAANRRTEIILAPKVDKLLDLVNGQ"
     gene            7758..8591
                     /gene="tatC"
                     /locus_tag="PLL59_07045"
     CDS             7758..8591
                     /gene="tatC"
                     /locus_tag="PLL59_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HMY23600.1"
                     /translation="MGNITDGKDKKEMGFFDHIEALRWHIMRSAIAVVAGALIAFTQK
                     KFIFDYLLFGPTNAQFPTNRFFCWLENNYTFFQGLCIKDAKFTFINTDITGQFMLHIQ
                     MALYVGAIGAFPYVFWEIWRFIKPALHEREIRNTRGIVFITSMLFTIGCLFGYFIIAP
                     FSINFLVQYQATSIVLNMIDINSYIGLLITMTIPTGILFELPMLVYLLTKLGILTPEF
                     MRKGRRISYVAILVVVAIITPQGDVTSLLLISAPIFLLYELSIGVSNRVYKQLVKEEA
                     Y"
     gene            complement(8588..9274)
                     /locus_tag="PLL59_07050"
     CDS             complement(8588..9274)
                     /locus_tag="PLL59_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HMY23601.1"
                     /translation="MQIILFYFLIGLLNVCAEFLVNEPLKYASKVLLMPLLMLFVYRT
                     IKTANVMRMIYAALFFSWLGDIFLMFPKTQTVISPKLLFLAGLISFLIAHIFYSLYFY
                     YEIKGKNTASIISKNPSLLLPFAIFVATLLYILFPTLDAMKVPVIIYTLVIITMAIMA
                     LNRHGFVESRSFVYVFIGACLFVFSDACIAINLFYKPFNLARLLIMSTYITAQFLIIK
                     GVILSKQMKA"
     gene            9355..10158
                     /gene="proC"
                     /locus_tag="PLL59_07055"
     CDS             9355..10158
                     /gene="proC"
                     /locus_tag="PLL59_07055"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HMY23602.1"
                     /translation="MNVLIIGGGNMGRSFAESFLNAKILTHKQLTVIEKDHAQIQELN
                     RMKLGQVYNDYGDFIVEKDLIIIAVKPQDAYAVYPKLQPFIQEKHIVLTIMAGVKIQE
                     VEQQLGTNKVIRCMPNLPCQIGLGVTGFMVSETIPQRDIDFIRKLLETTGISIQLYDE
                     DKLNAITAITGSGPAYIFYFMDAMIQKSMEYGFTLTEASKMVEQTFLGAVELYKVNDL
                     SCKEWINKVASKGGTTEAALKSFNQSAVSNNIQKGMDEALQRSRDLSKN"
     gene            10253..11494
                     /locus_tag="PLL59_07060"
     CDS             10253..11494
                     /locus_tag="PLL59_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HMY23603.1"
                     /translation="MERFTGILGIIAILGIAFLFSNNKKAINYRLVISGLALQLTLAL
                     LILKVPPVKSFFQTLGEGMKAIEGYAQQGAAFVYGGIMTDTHSPKGAIDALPYGAPGT
                     FVFAFSIPATIILVCVLVAILYHVGIMQRVVAAIARVMNFVMRVSGAEALSNVASAFV
                     GQVEAQVMIRPYLAGMTNSELLASMAGSMACIAGGILIVYVSMGVPAEYLIAASLMAA
                     PAALVISKIVFPETEESQTKGKVSVEIKSQYTNIIDAISHGASDGMKISVNVIAMLIG
                     FIALMALINALLGLIHPNLGLNTIFGYVFYPLAWVMGVPSEEVMKVATMMGQKLTINE
                     FVAYTSLIAEKATMSPKALAIASFALCGFANFSSVGIQIGGIGELAPSRRADLAKLGL
                     KALLCGTLASYLSATIAGMLM"
     gene            complement(11495..12760)
                     /locus_tag="PLL59_07065"
     CDS             complement(11495..12760)
                     /locus_tag="PLL59_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23604.1"
                     /translation="MKSQIEDKFRWYVLIAMLYFIYVLLMHKWGHQGDMTFWMNWSKY
                     IFTHGITHIYELENCNYLPAYLYVLWLHTHIQGNFQDIGDNLYTIKYFTLLFDFGAAY
                     FAVYFVPTMRDKIFYFFVLLLNAAFIYNTVFWGQVDAIYTCFGFVALIMAIKNRTVIS
                     IICLALALNFKLQALVFVPIITMILCFHLLQHKSYKKILIGILCAIVLQIFILLPFIL
                     HHQLPKIIAVITNTVGFYNFPTLGAFNFWSLMLPNVSIEGMYQISDNVKFGFLTYHQI
                     GLLLFFVLSFAALFPFLHFMYKQSFITKDIQFPIKYIFLITALCSLNFYFFNTQMHER
                     YVHPAIVLIATYSFLSKRYGIFLILSIAYFLNLERICWYLHLHNETYLNGLLFKPRLI
                     AVLYLFVILQSYYYLYFDKSKQETDISLA"
     gene            12832..14613
                     /gene="uvrC"
                     /locus_tag="PLL59_07070"
     CDS             12832..14613
                     /gene="uvrC"
                     /locus_tag="PLL59_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMY23605.1"
                     /translation="MSNFETLHTIIKNLPKNPGVYRYYDIDGNLLYIGKAKNLKSRVS
                     SYFVNKDHSFRIQIMVKKIHNIEYSIVPTEKDALLLENALIKEHQPKYNILLKDDKSF
                     PYIKILNEPFPRIFFTRKYEQDGAEYYGPYTSVNQVRSILELIKKLYPIRNCSLSLTE
                     KNIKAKKFKVCLEYHIGNCLAPCVGFQSKENYDNNIAQIRKIVKGRLHEVRQTLKEQM
                     QSYAEQMEFEKAELIKIKFHSLKEYISVSTVVNPSLGNFHIFGFAEDAQKAYINYLCV
                     YEGSIIRTKAITLQKKLDETKEELLNFAINDILSELKEKENILLPFELENENEQIQIL
                     VPKIGEKKKLIELAQRNAIFQLQKGKEKPKIDNTTRVLALMKEELKLNKIPRHIECFD
                     NSNFQGTNAVSACVVFKNAKPSKKDYRHFNVKTVIGANDFDTMKEVVYRRYHRMILEK
                     EALPDLVVIDGGKGQLSAAMESIMNLQLEDELQVISIAKRLEEIYYPNDPIPLHINKK
                     SDTLKVLQHIRNEAHRFGITFHRNKRDKNTLSTELQTIAGIGEATATELLRIFGSIKR
                     IKQTEKIELEKAIGKAKAKLVFDYYHK"
     gene            complement(14688..15647)
                     /gene="gldN"
                     /locus_tag="PLL59_07075"
     CDS             complement(14688..15647)
                     /gene="gldN"
                     /locus_tag="PLL59_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HMY23606.1"
                     /translation="MKNIGYVLLTASLLNFTYSFAQDGDSTVNSSSTQPTTTEDPNAG
                     STVQPISLPPAAATASTVDTNNLKTEVQKLPWMQEITGKRQAMSYEPIREADVFWWKN
                     VWRVIDLREKINLHFKWPKAPFIQVLLDAVQAGKCQVYSGMDDDFSAPIDPKEALSVA
                     GGGVDSIYVTDPGTGIHTLQVINKEVNWNNVNKLRVKEVWFFNKQTSTMEVRIMGVCP
                     LYDNYDPATGDFRGEVPVFWAYFPSLRQTLVNTEAFNPFPNGVRLNWDQVFAVRLFGS
                     YIFKVDNVQDFRIKDYKSGIDVLYESEAKKQELFNFEHDLWEY"
     gene            complement(15675..>16438)
                     /locus_tag="PLL59_07080"
     CDS             complement(15675..>16438)
                     /locus_tag="PLL59_07080"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HMY23607.1"
                     /translation="SKSAGTVTVNVNGQNVPVDQTTGIAKYKASPGVGEYPLRGSVSV
                     RNERTGEVKSYPLPEFKYTVAAPFATVSPTKMNVFYIGVPNPVQVSAAGVANSKLQVS
                     MSAGSISGSAGKYEARVTTQGTTNVTVSANGKSYGSFPFRVKLIPNPIAKVSGQPGGR
                     INAGVWKAQQGVVADLENFDFDAKFEVLSFNMFYQPKLQDPGIASATGPYFSAAQKAF
                     IAKAKPGDIFYIEEIKVKGPDGLSRKIPGVAFKID"
BASE COUNT         5732 a         2875 c         2823 g         5008 t
ORIGIN      
        1 aaaaacctat tcttgcttta gatcctggat ttgctactgg ttgcaaatta gtttgtatag
       61 atgataaagg tacactctta gaagacagtg tgatttatcc gcatcctcct ttaaataaaa
      121 agagagaggc ggaagatata gtgcgtggct tgctatataa atacaacata aacaccattg
      181 ccataggaaa cggcactgct ggaagggaaa ccgaagattg gttgaataag atatcatggg
      241 agcgaaaacc acgattgttt tctgttaatg aaagtggcgc ttctatctat tcagcatctg
      301 atgtcgcgcg agaagaattt cctgataaag acattacagt tcgtggtgca gtatctatag
      361 gaagaagatt aatggatcca ttagcagagc tcgtaaaaat tgatccaaag tcaattggcg
      421 taggtcagta tcagcatgat gtcaatcaat atttactaaa gcaatcactc gatgaaacag
      481 tagtaagctg tgtaagccaa gttggcgtaa acattaacac tgcaagcaaa cacctgctga
      541 tgtatgttgc tggtttaggt caaagtacgg ctgaaaatat tatacaacac agaacgaata
      601 atggcatttt taaatcgaga aaagaactgt tacaagtaag tagattagga gaaaaaatat
      661 tcgaacaagc agcagggttt ttacgaattg ctgatgctca aaatccatta gacaatagtg
      721 ctgtgcatcc tgaaagatat gcattggtag agcaaatggc acatgaccaa aatgtgacca
      781 tacaagaatt gcttgcgaat aaagatgtca gaaaaaaaat tgacttaaaa aaatatattt
      841 ctgaagaagt aggcttacct acattacaag atattttgaa agaattagaa aaacctggca
      901 gagacccgcg cgacgaaatc caagcttttg tgtttgatgc aacaataaaa acgattgaag
      961 acttacacac aggcatggtc gtcccaggta ttgtcactaa tattactaat ttcggttgtt
     1021 ttgtcaatat tggtgtaaag caagatggat taattcatat ctcagaatta gccaataaat
     1081 ttatcaaaga cccaaatgaa atagtaaaac tacaacaagc tattcgagta aaaattattg
     1141 aagtggatct aagtagaaaa cgcatcgttc ttagcaaaaa acaagcagaa taaaacaaca
     1201 ttatttttta taaaattaca attgctctgg tctattttat aactttataa aatgacattc
     1261 gttgcaatag attttgaaac agcgacttcg caaccttatt ctgcttgtgc cgtcggaatt
     1321 atatctgttc aagacagtgt gataactgac gagtttgaat gtttgattca gcctccgaat
     1381 aatctatatt ttcagcaaaa tacaaaggtt catggcttgt ctgcaaatga cactaaacat
     1441 gcgctagcat ttgacaatgt atttgtagaa atacagcagc gattaatagg aaaacaagtt
     1501 gttgcacaca atgaaagttt tgatcgaaat gtattgaaac aatgcattgc tcattatcaa
     1561 ttatcatcaa aaggattact gcttcatcga aattggaatt gcaccgtaaa actatcacag
     1621 catttaggtt attcgccaaa taaacttagc gattgctgtc aacggtttaa tattccacta
     1681 caacatcatg atgcattgtc tgatgcacgt gcatgtgcta ttttattcat gcaattatct
     1741 gcacaacaaa gaaaaaataa gatgtatctg ttttaagaaa aaatgaaatt aaggaacagc
     1801 atttatcgaa tcaactattg ctttggcagc accaatttca taaaaatcaa cacctaaaaa
     1861 attgacaaaa tggctattgt ctgttgcgca atcttggaca cgcttgccaa cgactgctct
     1921 cgcatttgct tgaggcgcta aatttttatc tggcaaacct aacacatttt gcaaccaatg
     1981 attgattaaa tacaattcct ttgtaccgct tccaccacgg ttgacatcag tatcaaactg
     2041 acttctattt tgataagaat atttcgtgtc aaatatcata gaccacgcat aatgtaaata
     2101 actaattgaa ggtaatttgc taaattctgc aaacaatact aagcgttgat tgctatcaat
     2161 catcgattgt aatgttgccc aattggctgt attatgtgta tatgtcattg cactcagtcc
     2221 actactatca attgcctttt gcaattgtgc attgctgcct tcattttgaa aaataatgct
     2281 cactacactt ttaggattgg tcaccataaa atcgtttacc tcttttagca catctactaa
     2341 attttgcgaa cccattgctt caatagcatg atatgttttt gcaacaccac cagcaccgtc
     2401 ataagtgtct atcattaaac cacgtacacc gttttgcaat tgatgtgtaa tagagtgtgt
     2461 ttgatttgga atggcgtacg atttctccga attattcatc gcattatgtg tcatcaggaa
     2521 acaaacttca tcgtaatgct tctttaataa tgtgtcttct ttgtcatcat tttttgtttt
     2581 agtacaagct acaataaatg caaaagagaa aatcaaaata tttatcgagc aaaaagcata
     2641 tcttttcata agcaatgtat attttgtaaa agtcaaattt ataataaatc gagcataagt
     2701 ataaattttt taatcgattt ttattaatat agtatcactt tatggctacg caaaagaaaa
     2761 atatcatatc aaaaattttt gacgaccaac ggatacaaga atatgtgaac gtatttccta
     2821 ataacgttgg ctcttttgga tttgatgctt ggggttttaa tatcaagaat atgaaaaaca
     2881 acatgcgttt taccaaatat ttgtatgaag aattttacaa agtagaagct tttggtttag
     2941 aaaatattcc taaagatggc agatgtttaa ttattcctaa ccacagcggg caattgccat
     3001 ttgatggcat gctggtaggc tacgccatga ttactaatga aaaaggacca cgagcaccaa
     3061 aggcaatggt ggagcgattt ctaccaaccg ttccgtttat tggcaattgg ctaaactccg
     3121 ttggtgcggt tattggcgac ccgataaatt gcgagcgaat gcttgaaaga gaagaagcta
     3181 ttgttgtatt tccagaaggc gttcgcggct ctaataaatt atacaaacac aaataccaat
     3241 tgcaacgctt tggtgctggc tttatgcatt tagccatgaa acacgatgca cctattattc
     3301 cagtaggaat tgtaggcatg gaagaaacta tacgctccta cgcagacatt aaaccattag
     3361 ccaatttatt gggcatgccc gttgcacctt tagttattcc atttattttt ccatcaaaag
     3421 tttatctctt atttggaaag cctatttatt ttaaaaatga cacttataaa gaaagtgaaa
     3481 taaatgcgcg agttgatata gtaaaagacg caatcaatga attgctaaaa gaaggattag
     3541 ccaaacgcga agcatatata caagaaaaga agcaaaaaaa ataagtaaat actagcaata
     3601 acctaaacaa tatattatgg aaccgagaaa gaaaatttta gtaacaggtg ctgcgggtgc
     3661 attagcaaaa aaagtaatcg acaaacttaa aaaagattat aagattattg cggttgactt
     3721 tagaacaaaa gtagatttag ggattcctgt agagagctat aaagtggatt ttaataaaag
     3781 actttttgaa gatattttcc gcgaacatca gtttgacggc attatacatt tgggaagaat
     3841 atctgcacag gaattaaata gatttacgcg ttataatgcc aatgtaattg gtacaaaaaa
     3901 attattcgat ttagcacgca aatacaatgt gccaaaaaca ttggtattgt ccacttattt
     3961 cgtttatggc gcatcagcat ataatccgtc cttgctcgac gaaacaacgc cattaaaagc
     4021 atcagagctc acaatggatt tagtagattc tgtcgagttg gaaaatttat caaccatcta
     4081 tttatacaaa catcctgaac ttaatattac tgttttacgt ccgtgcaata ttgctgggcc
     4141 tggtgtacgc aatactttct ctttactatt atcggaaaaa cgttcgccag ttttgtgggg
     4201 tttttcgcca ttaatgcaat tcatacatat cgatgatatg cgcgatgcca tttgtacagc
     4261 atttgaaaag aactttcctg gcgtgtataa tgtagcacct gatgattata tcggcttcca
     4321 agacgctatt aaagcagctg gctcaagtac tatacctatt ccatcaattc caccatcatt
     4381 aacagaatct atttctaagt ttttaaaatg gaaatctttt ccgccatatc tcattaatta
     4441 cttcaaatat cctgttatta ttgatggaag tttgtttgcc aaaactttca acttccgacc
     4501 aaagaaaacg ctatccgata tttttaccta ttatagaaat ttgaaataat taataataca
     4561 aaaaattgta ttttgtttta taaactcgat atgcgaacat tactgctctc tctactttta
     4621 attgcttgta ccatacattt acaagctaaa gaaaatacat tttttgccgg tataaaactc
     4681 aacaacggca gaaaaattga tgctataaaa aatagctttg caaacggaac atttcgtgac
     4741 aaaagttatt tcttaagcaa tacctattat tttggtggca ctataaaaaa tgtaagtatt
     4801 tcatgcggta ttgcatataa cttatatcat tacaatgaca aaagagacta cttatttcta
     4861 ataatcggtc caaactatca gaaaaaaaac aaccacatat tatctattcc atttaatgta
     4921 gattataata ttaatattta caaagagaaa ttatttgtta ttgttggcgc aggcatcgaa
     4981 tatacaatta ccttatcaca agttttaaaa ccaaataatg gcactgtatt tcctgatgta
     5041 aaagtgaagc acaaggcttt aaataatacc tataaaacac acgcgctgct ttttaatttc
     5101 cgaagcggat tgttatatgc tgtcaataaa agagtggaca tttttacctc atttgatttt
     5161 ggtatttatg ctaataaata tatggcattg cgcaatgatt atccaagcgc taataaaaaa
     5221 tacacctatc tgttaagcaa tagtattggt gtggctataa aatttgctaa actaaaaagt
     5281 aaatcaaaaa attagaaaag tagtttgtaa aattctgcta ccgattttac taattgtatc
     5341 gttgcgcttt cgctttttac actttgattg tatttagata taatgatttt ttcgtagcct
     5401 aattttccag cttctgctat tctttgttct actttgttta ctgctctaat ttcgccgctc
     5461 aatcctactt caccgctaaa agaatattta ttatttattg caatatcttc atacgatgac
     5521 aacaaagcag ctaccacagc taaatccacc gctggatctt ccacgcgcaa accaccggcg
     5581 atattaagaa atacatcttt tgcaccaaat cgaaaaccac atcttttatc caagaccgcc
     5641 aaaagcatac ctaatcttcg caaatcgtag ccagtagcac tgcgctgtgg cgtgccatac
     5701 actgctggcg acactaaggc ttgcacctct accaacaagg cacgcatacc ttccatcgtt
     5761 acggcaatgg caacgccact cacctgttca tctctttgtg taattagcaa ttccgaagga
     5821 ttgctgacag cgcgcatgcc atcgccacgc atttcgtaaa tacctaattc tgctgttgaa
     5881 ccaaaacgat tcttttgtgt acgcaaaatt ctataattgt aatttctatc accttcgaac
     5941 tgcaagaccg tatccaccat gtgttctaat atttttggac cagcgattgt gccttctttt
     6001 gtgatgtgcc ctacaataaa aatcggcaaa tgatgtcgct tcgcatattg ctgcaacaca
     6061 tgtgtcgctt cacggatttg cgaaacgcta ccagcagcag attctattaa tgatgtttct
     6121 aaagtttgta ttgaatctat aactaataat tgaggttgtg tttgttctat ttgttgtaca
     6181 atcgactcta cagatgtttc tgtgtaaact aaaatatccg aattttgttg atgtattctg
     6241 tcagcacgca ttttcaattg ttgctcgctt tcttcgcctg aaacatataa tgttttcaag
     6301 ccttttgttt gcaatacaac ctgcaataac aaggtcgatt ttccgatgcc aggttcacct
     6361 cctatcaata tcaatgagcc gggaactatg ccaccaccta acactctatt taactctggg
     6421 tctgatgtta taatgcgttg tctgttttcc gacgaaattt ctgttaacag ctgtggcttg
     6481 tttttaggtg cattcacaac agctgcgctt tttgactttg gctctattaa ctcttctaca
     6541 taactattcc attcgccaca attattgcat tttcccatcc attttggcga gttggtgcca
     6601 caattctgac aaatgtatat gctcttggtt ttagacatgt gtgtaaagat aagcatgtat
     6661 tatcgtaaat agaaagctga tatttgtaat aaattatttt tatgaaaaga agtttgctct
     6721 acttgtttgt tgccatcgta tttaccaact gcgtcacgca aaaagaatat gacaaagctt
     6781 tgaatgatta tttaaatgaa gccataaaaa ataaatattt gaatgagtct ttagattctt
     6841 taaaggctga ttgcacgacc aaaaacactc aactttcaac gcaaattgaa caattaagag
     6901 ccgactccag cgaattggca acattgattg agtcgatgaa aaaagaaatc gatgagcaag
     6961 cgcttgaata tgctaaacaa caacaagaat atctcaaaaa attgaaagat gctggcgata
     7021 aaaatcagaa aacaaatgcc gatttaattc aaatgcaatt agatttagaa aaacaacgta
     7081 tcgcattaca acaaaaagaa accgacctta aaaaagcaaa tactgatttg ctagcgcgtg
     7141 aggccaagat aaatgaactt gagaaactat taacagaaca aaaaacaaaa gcagatgcgc
     7201 tgcgtgatgc cattaaaaaa gcattaactg atttttctgc tggcgaatta agcgtgtaca
     7261 ccaaagacgg aaaagtatat gtgtcgatgt cagataaatt attgttcaaa tcaggctcta
     7321 cgacggtaga aagcaaaggc gttgatgcat tgggaaaact ttcagatgtc atcaaaaaaa
     7381 atcctgatat tttagtaaat attgaaggcc ataccgataa tgtgccttat ggaacaggga
     7441 gcggccttat caaagataat tgggatttga gcttgatgcg ttcaagtagt gtgctacata
     7501 ttttaacaga caaatataaa gtaagctcct tgcaagtcat tgcatctgga cgtggcgagt
     7561 tttcgccgaa agaaagcaat agcaccgccg acggcagagc agccaatcgt agaacagaaa
     7621 ttatattagc gccaaaagtc gataaactat tggatttagt aaatggtcaa taaaaaatcg
     7681 taattgtttt tctaaaaatt tattgactaa aattaatatt tcgttgatat acaaagcagt
     7741 aattttgtgt tttcaacatg ggcaacatca cagacggaaa ggataagaaa gaaatggggt
     7801 ttttcgacca catcgaagca ttgcgctggc acatcatgcg ctctgccatt gctgtggttg
     7861 ctggtgcatt aattgcattt acacaaaaaa aattcatttt tgactattta ctgtttgggc
     7921 caacaaatgc acaatttccg acaaatcgtt ttttctgctg gctcgaaaac aattacacat
     7981 tttttcaagg tttatgtata aaagatgcca aattcacttt catcaataca gacattacag
     8041 gtcagtttat gctacacatt caaatggcat tatatgttgg tgctatcggc gcatttcctt
     8101 atgttttttg ggaaatctgg cgatttataa agccggcact acatgagcgt gaaatcagaa
     8161 atacacgtgg catcgtgttt attacatcaa tgctatttac aataggttgt ttgtttggat
     8221 attttattat cgcacctttt tctatcaatt ttttagtaca atatcaagca acatctattg
     8281 tcctcaatat gattgatatt aacagctaca tcggcttatt gattaccatg acgataccaa
     8341 caggcatttt atttgaatta ccaatgttgg tgtatttact aacaaaatta ggcatcttga
     8401 cgccagaatt tatgcgcaaa ggcagaagaa tttcatatgt cgccatatta gttgttgttg
     8461 ccattatcac accacaaggt gatgttacaa gcttattgct tatctctgcg cctatatttt
     8521 tattgtacga attaagtatt ggcgtttcta atagagtgta caaacaattg gtgaaagaag
     8581 aggcttatta agccttcatt tgctttgaca atatcacacc ttttataatt aaaaattgtg
     8641 cagttatgta agtactcata atcagcagtc gagctaaatt aaaaggttta taaaataaat
     8701 taatggcgat acaagcatcg gaaaacacga ataaacacgc accaataaac acatatacaa
     8761 aagaacgaga ctctacaaat ccatggcgat taagtgccat tattgccatt gtaatgatga
     8821 ctaaagtata aataattact ggcactttca tcgcgtctaa agttggaaat aaaatataca
     8881 gtagtgttgc aacaaaaata gcaaatggca aaagtaagct tggatttttg cttataattg
     8941 atgctgtatt ttttcctttt atttcataat aaaaataaag gctataaaaa atatgagcaa
     9001 ttaaaaagga gattaaacca gctaagaaaa gcagctttgg cgatatgact gtttgtgttt
     9061 ttggaaacat taaaaagata tcgcctagcc acgaaaagaa tagtgccgcg taaatcatac
     9121 gcattacatt agctgtttta attgtgcgat atacaaacag cattaataga ggcatcagca
     9181 atacttttga agcatatttt aatggttcat ttaccaaaaa ctcagcgcat acatttagca
     9241 agcctatcaa aaaataaaat agtataattt gcatatttga atatagtaaa aaaaacaaaa
     9301 atcatttcta attatcaata gcaattattc acaactaaat actattttta tgacatgaac
     9361 gtacttatca ttggcggtgg aaatatgggc agaagctttg ctgaaagctt tttaaatgct
     9421 aaaatattaa cacataaaca gctcacagtt atagaaaaag atcatgcaca aattcaagaa
     9481 ctgaatcgca tgaaattagg tcaagtatat aatgattacg gtgattttat agtagagaaa
     9541 gatttaatca tcattgccgt aaaaccgcaa gatgcgtatg ccgtatatcc aaaattacaa
     9601 ccatttatcc aagaaaaaca tatcgtgctt accattatgg caggcgtgaa gatccaagag
     9661 gtagaacaac agcttggcac aaacaaagtc attcgctgta tgccaaacct accatgtcaa
     9721 atcggattag gcgtaactgg ttttatggta tccgaaacca taccacaacg cgatatcgat
     9781 tttatccgca aactattaga aacaacagga atttcaattc aattatacga tgaagataag
     9841 ctgaatgcca tcacggcgat tactggctct ggccctgctt atatttttta ttttatggac
     9901 gccatgattc aaaaatcaat ggaatatggt tttacattaa cagaagcatc aaaaatggtg
     9961 gaacaaactt ttttaggtgc tgtagagctt tataaagtca atgatttatc gtgcaaagaa
    10021 tggataaaca aagtagcctc taaaggcgga accacagaag cagcattgaa atcttttaat
    10081 caatcagcag ttagtaataa catacaaaaa ggtatggacg aggcattaca gcgcagtcgt
    10141 gatttaagta aaaactaaag caaacaagca tttcttactg ttgatatttt atcatcattg
    10201 caatacgaat aacgtcgaca aaaaagtata tttgttatac atacaacaat ttatggagag
    10261 atttacaggt attttaggca tcatcgctat tttaggtata gcatttctat tttccaataa
    10321 taaaaaagcc atcaactaca gattagtcat cagtggtctt gcacttcaat taacactagc
    10381 tttgctaata ttaaaagtgc cacctgtaaa atcatttttt caaacgctgg gcgaaggcat
    10441 gaaagccatt gaaggctatg cgcaacaagg agcagcattc gtttatggtg gcattatgac
    10501 agacacacat tcaccaaaag gcgccatcga tgctttgcct tatggtgcgc ctggcacatt
    10561 tgtgtttgcc ttcagcattc ctgcaacaat tatcttagtt tgtgtattag tagccatttt
    10621 atatcatgta ggcattatgc agcgtgtagt ggcagccatt gctcgcgtaa tgaattttgt
    10681 aatgcgtgtt tctggagctg aagcactcag caacgtggca agtgcttttg tagggcaagt
    10741 ggaagcacag gtaatgatac gtccttactt agcaggcatg accaatagcg aattattagc
    10801 atcaatggct ggcagtatgg catgtatcgc tggcggaatt ttaattgtat atgtgagtat
    10861 gggtgtgcct gcggaatatt taatagcagc cagcttaatg gcagctcctg ccgcattagt
    10921 aatctctaaa attgtatttc cagaaacgga agaatcacaa acgaaaggaa aagtaagtgt
    10981 agaaattaaa agtcaatata caaacatcat cgatgctatt tcacacggcg caagcgatgg
    11041 catgaaaata tctgtaaatg taattgcgat gctaattggt tttattgcgt taatggcatt
    11101 aatcaatgcg ctattaggtc taattcatcc aaacttaggc ttaaatacca tctttggcta
    11161 tgtattttat ccattggcat gggtcatggg cgtgccgagt gaagaagtga tgaaagtagc
    11221 aaccatgatg ggacaaaaat taaccattaa cgagtttgtt gcttatacca gtttaattgc
    11281 agagaaagcg acaatgtcgc caaaagcatt agccattgca agcttcgcat tatgtggatt
    11341 cgccaatttt agttccgtag gcattcaaat tggaggcata ggagaattgg cacctagccg
    11401 acgtgccgac ctagcaaaac tcggcttaaa agcattactt tgcggtactt tagcttctta
    11461 cctttctgca acaattgctg gaatgttgat gtaatcaagc aagtgaaatg tctgtttcct
    11521 gttttgattt atcaaagtat agatagtaat acgactgtag tataacaaac aaatacaaaa
    11581 cagcaattaa tcttggttta aataataagc cgtttaggta cgtttcatta tgtaaatgta
    11641 agtaccaaca tattctttct aagttaagaa aataagcaat agataaaatt aaaaaaatgc
    11701 catatcgttt cgacaaaaaa ctatatgtag cgataagcac aatcgccgga tgcacatatc
    11761 gttcatgcat ttgtgtatta aaaaaataga aatttagaga acacaatgct gttatcagaa
    11821 aaatatattt gattggaaat tgaatatctt ttgtaataaa agattgctta tacataaaat
    11881 gcaaaaaagg aaataatgcc gcaaatgaca acacaaaaaa caagagtaaa ccaatttgat
    11941 gatacgtcaa aaaaccaaat ttaacattat cacttatttg atacatacct tctatagaga
    12001 cattcggcaa catcaaagac cagaaattaa atgcacctaa agttgggaaa ttataaaatc
    12061 caactgtatt tgtaattact gcaataattt taggcaattg atgatgaagt ataaacggca
    12121 ataaaataaa aatttgtaaa acaatagcgc ataaaatacc aattagtatt ttcttgtatg
    12181 acttgtgttg taataaatga aaacacaaaa tcattgttat aattggcaca aacactaatg
    12241 cttgtagttt aaaattcaat gccaatgcta aacagattat actgattaca gttctatttt
    12301 taatagccat aattaaagca acaaaaccaa agcatgtata aatagcatct acttgtcccc
    12361 aaaatacagt attataaata aatgcggcat tcaacagtaa tacgaaaaaa taaaatattt
    12421 tatctcgcat cgttggtaca aaatatacag caaagtatgc tgcgccaaaa tcaaaaagca
    12481 aggtgaaata tttaatggta tatagattat cgccaatgtc ttgaaaattt ccttgaatgt
    12541 gcgtatgtaa ccataataca tataaatatg ctggcaagta attgcaattt tctagctcgt
    12601 aaatatgcgt aattccatgc gtaaatatat attttgacca attcatccag aatgtcatat
    12661 cgccttggtg gccccattta tgcatgagta gtacatatat aaaatatagc attgcgatga
    12721 gcacatacca tctgaattta tcttctattt gcgatttcat gttatatctt ttcaaatata
    12781 cctttttagt tttaatataa acatcatcag catcgcttat atttgtatcg catgtcgaat
    12841 tttgaaacac tacatactat cattaaaaac ttacctaaaa atccaggtgt ttatcgttat
    12901 tatgatattg atggaaattt attatacatt ggaaaagcaa aaaatctaaa aagtagagtt
    12961 tcatcctact tcgtgaataa agaccattcc tttcgcattc aaataatggt caaaaaaatt
    13021 cacaatatag aatactcaat cgtgcctact gaaaaagatg ccttgttgct ggaaaatgca
    13081 cttatcaaag aacatcaacc aaaatataat atcttattaa aagacgataa atcattccca
    13141 tatatcaaaa tattaaatga gccatttcca cgaatatttt ttactagaaa gtatgagcaa
    13201 gatggtgcag agtattatgg tccatatacc tcagtcaatc aagtgcgttc aattttagaa
    13261 ctgataaaga aattatatcc aatacgaaat tgctcacttt cgctgacaga aaaaaatatt
    13321 aaagcaaaaa aattcaaagt ctgtcttgag tatcatattg gcaattgctt agcaccctgt
    13381 gttggttttc aaagcaaaga aaattatgat aataatatag cacaaattcg caaaattgta
    13441 aaaggcagat tgcatgaagt acggcaaact ttaaaagaac agatgcaatc ttacgcagaa
    13501 caaatggaat ttgaaaaagc agaactcatt aaaataaaat ttcattcact gaaagaatac
    13561 attagcgtat caaccgtagt caatccaagt ttaggaaatt ttcatatatt tggatttgca
    13621 gaagatgcgc aaaaagcata catcaattat ttgtgcgtat atgaaggcag catcatacga
    13681 acaaaagcca ttaccttaca aaagaaatta gatgaaacaa aagaagagtt attgaacttc
    13741 gcaataaacg acatcttaag cgaactgaaa gaaaaagaaa acatcttatt gcctttcgaa
    13801 ttagaaaatg aaaatgaaca gatacaaatt ctagtgccaa aaattggcga gaaaaagaaa
    13861 ttaatagaat tagctcaacg caatgctatt ttccagcttc aaaaaggaaa agaaaaacct
    13921 aagatagaca acacaacacg tgtgttggcc ttgatgaaag aagaattaaa actcaataaa
    13981 ataccaagac atattgagtg ttttgacaat tcaaatttcc aaggtacaaa tgctgtatct
    14041 gcttgcgttg tctttaaaaa tgcaaagcca agcaaaaaag attacagaca ttttaatgta
    14101 aaaacggtaa tcggcgcaaa tgattttgac accatgaaag aagtcgtata tcgacgatat
    14161 catagaatga ttttggagaa ggaagcattg cctgacttag tagtcatcga tggtggaaaa
    14221 ggacaattaa gtgctgctat ggaaagcatt atgaatttac aattagaaga tgagttacag
    14281 gtaatatcta tagccaaacg cctagaagaa atttattatc cgaatgatcc gattccgttg
    14341 catatcaata aaaaatcgga tacattaaaa gtattgcaac atatacgaaa cgaagcacat
    14401 cgatttggca ttacatttca tagaaataaa agagataaaa atacgcttag cacggagttg
    14461 cagaccattg caggaattgg agaagctaca gcaactgagt tattgcgcat atttggttcg
    14521 ataaagagaa taaaacaaac agaaaaaatt gaattggaga aagctattgg gaaagcaaaa
    14581 gcaaaactag tatttgatta ctatcataaa taaaaaaaga ttttgtagca tacaattaaa
    14641 aaaatgtctc gccgatggcg agacattttt ttataaaatc gtattgatta atattcccac
    14701 aaatcgtgtt caaaattaaa caattcttgt tttttagctt cagattcata caagacatcg
    14761 atacctgatt tataatcttt aatacggaaa tcttgaacat tatctacttt aaagatgtag
    14821 ctaccaaaca aacgaacagc aaatacttga tcccaattta agcgaacacc attaggaaaa
    14881 gggttgaatg cctctgtatt tactaatgtt tgacgtaagc ttgggaaata agcccaaaac
    14941 acgggaacct caccacggaa atctcctgta gctgggtcgt agttatcata taatggacaa
    15001 acacccatga tacgcacttc catagtagaa gtttgtttgt taaagaacca aacttctttc
    15061 acgcgtaatt tatttacgtt attccagttt acttctttat tgataacttg caaagtatga
    15121 ataccagtac ctgggtcagt aacatagatt gagtcaacac caccaccagc aacgcttaac
    15181 gcttcttttg gatcaatcgg tgcagaaaaa tcatcatcca taccgctata cacttggcat
    15241 tttcccgctt gaacagcatc taacaatacc tgaatgaaag gagctttcgg ccatttgaaa
    15301 tgcaaattaa tcttttctct taggtcaata acgcgccaaa catttttcca ccaaaataca
    15361 tctgcttcac gaattggttc gtagctcatc gcttgtcttt tacctgtaat ctcttgcatc
    15421 caaggcaatt tttgcacctc agttttcaag ttattggtat ctactgttga tgctgttgca
    15481 gcagcaggtg gtaaagaaat tggctgaact gttgagccag catttggatc ttcagtcgtt
    15541 gttggttgtg ttgacgaact attgacagta ctgtctccat cttgcgcaaa agaataagtg
    15601 aagtttaaca acgaagctgt tagcaacaca tatcctatat ttttcatttg cattttcata
    15661 taactaaatt cttattaatc gattttaaag gctacacctg gaattttacg agacaaacca
    15721 tctggacctt ttactttaat ctcttcaata tagaagatat caccaggttt tgctttagca
    15781 ataaatgctt tttgcgctgc gctgaaatat ggaccagtag cagaagcaat accaggatct
    15841 tgtaatttag gttgatagaa catattgaaa cttaatactt caaatttagc atcaaagtca
    15901 aagttctcta aatcagcaac tacaccttgt tgtgctttcc atacaccagc attgattctt
    15961 ccgccaggtt ggccagaaac ttttgcaatt ggatttggaa ttaacttcac acggaatgga
    16021 aaagaaccat agcttttgcc attagcagaa actgttacat ttgtagttcc ttgtgtagtt
    16081 acgcgagctt cgtatttacc ggctgaacca gatattgaac cagcagacat actaacttgt
    16141 agtttagaat ttgcaacacc agccgcagaa acttgaactg gatttggtac gccaatatag
    16201 aatacgttca tttttgtagg tgaaaccgta gcaaacggtg ctgcaacagt atatttgaat
    16261 tctggtaatg gataagattt tacctctcca gttctttcgt ttctaacaga aacggaaccc
    16321 cgtaaaggat actcacctac acctggtgat gctttatact tagcaatacc tgtagtttgg
    16381 tcaactggta cgttttgacc gtttacattt acagtaactg taccagcact tttagatg
//
LOCUS       DAOQQV010000055        16220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1124_length_16220_cov_9.132509, whole genome shotgun sequence.
ACCESSION   DAOQQV010000055 DAOQQV010000000
VERSION     DAOQQV010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16220
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1124_length_16220_cov_9.132509"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="PLL59_07085"
     CDS             <1..1462
                     /locus_tag="PLL59_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HMY23608.1"
                     /translation="ISVVAAQEQRRGNDPFADMMEEMEEMQRQMMRQFQQPQYSEAEI
                     KQYLAKNLFVRVIPTKSTLYQGEQTTLSYKIYARVSCNGFMADKMPSYNGFLSEEFKL
                     PEQQEPKIEVLNGVEYQTLEVKRVTLFPQKTGKLNIEPLALTTNAYIGYQPYEYSFKS
                     NTITLDVKPLPEKNKPLTFSGAVGKFSFEADYDKTKTSVGEPITLKITYTGTGNLKLI
                     TAPKLEFPEEFESFEPKTKDNYENNGNVVSGSKTFEYVLIPQDGGTFKLPKYEFAYFD
                     VDKGDYVKFVLPETEITVSGKAKLSENVANFFKREKQDKPRSIYAIKKVYVKNKSFAA
                     SNIFWTLAITPIVFLIIAFVFRKRDYSSTELIALRRKKANKIALQRLSIAKKLLQQKQ
                     EEAFYNEVVRALWHYLSDKLYLPQSELSKENISEKLHLRAIEQSKINDLHNTLDTCEQ
                     ALFSPIGQEMAMQQTYTKAIELIIDLEEQLKKDSKI"
     gene            1481..2233
                     /locus_tag="PLL59_07090"
     CDS             1481..2233
                     /locus_tag="PLL59_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY23609.1"
                     /translation="MSKHSKYILLVFFYLIFGFTKAQNNISLFQTANKYYQNKQYEEA
                     EKMYLLLLKKENNNANANYNLGNTYFHLKQYTNAILYYEKAKKIEPDNKQIQHNIDLT
                     NNKLFSKIEFSKTFFVAKKIKGFAHTKSADTWSIYLLVSLWIGIVLICIHFLKNKQMA
                     FNIGATILLFAMLFAYFTYTTYKSEQLQDFAIVMTNDATLNKMPVANIGNTDSIQSGT
                     KVQIIDSDKSWLKIKLPNDKIGWISIDKIARI"
     gene            complement(2230..2913)
                     /locus_tag="PLL59_07095"
     CDS             complement(2230..2913)
                     /locus_tag="PLL59_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23610.1"
                     /translation="MKRGLFFLFFGFLFFCVQAQDDIVLDLDSIKNIKTTRYSSINEV
                     GVGIHVAGKLIQKFPDITKKTTLQVEKPSFIFRTVHGALINPKLFIGAGLGIDFRPNG
                     TNGSRFYYFTFPIFAEFREYFLEGNFNLFLSERVGGAIYIDSYFNSQINKGRYAGAFG
                     EFMIGGRYVTNSRKLAIHFGAGYRLQHLQRKVDIQSLNGSGGINQTYANTPEITIKHY
                     VPITIGVTF"
     gene            3049..4470
                     /locus_tag="PLL59_07100"
     CDS             3049..4470
                     /locus_tag="PLL59_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23611.1"
                     /translation="MLAQTFEYSYNCKQAYQNIYKLKFNEGQQWLAQEKADNPKNVLT
                     YFIENHIDFLKLYINDNRKLYAAIEPKMEQRLEKIKKGNPVSPYYLYAQADIHLQWAF
                     CKIKFGDYLSAIFEVKKSFSMLQENKKRFPAFKPNIKSLGLLHTLFGAVPDKYKFGAK
                     LLGLKGSIDEGMQELNSVLQDSSFQFKEETIILYTLLLLHLQKDKTNAWNMIENTTIP
                     LGDNLLNHFIAASVAEHTGKNDKMISILANRPNSAAYYAFPYLDFLYGFAKLNRLDAD
                     ADVYLKKFITTNKGRAYQKEALRKLAWFYLINDNPTGYKKYMQQILTISEAPSDEDKS
                     AQKEAEQNFIPNKELLKARVLSDGAYFNKALATINAIAPEKLTRTRDIIEYYYRKARI
                     YDELKQNTEAVKYYLISIQKGENYKYYFAANACIKLGAIFEEQHKKSNAIQYYKKAIQ
                     LDKDEYSNSIDAEAKAGLNRLGN"
     gene            4539..5570
                     /gene="murB"
                     /locus_tag="PLL59_07105"
     CDS             4539..5570
                     /gene="murB"
                     /locus_tag="PLL59_07105"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HMY23612.1"
                     /translation="MHAMVSIQKDISLLPYNTFGIDVNASNFCRVSSKEALISLINQA
                     IFPISDWLIIGGGSNLLFCKNMEGLVVKNEIIGIELIKENETHVWLKSYSGTIWHELV
                     MYCVENNLGGIENLALIPGTVGAAPMQNIGAYGVEIKDTFVEATAIELSTGDTYIFGK
                     DACNFGYRESIFKKEAKGKYFIYDVTFKLSKSPQLNMAYGDIQKIIKEKNIATPSIKD
                     VADAVIQIRESKLPNPKDIGNAGSFFKNPEISIEQFSLLQQQFPNMPHYSLSNGKEKI
                     PAAWLIEQAGWKGKQIGNTGNHAKQALVIVNYGHATGAEIWQHALLVRDDVQQKFGII
                     LEAEVNIIA"
     gene            complement(5604..6497)
                     /locus_tag="PLL59_07110"
     CDS             complement(5604..6497)
                     /locus_tag="PLL59_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23613.1"
                     /translation="MAQKGGTLSIGATPGLSYMLAQNTYFLAENSKELDYKPKFSYHV
                     FIQGGYNFKEQHGIVAFANFCDEGQNYKDEFKWKKYPSLIGTHRKSVDFKYMGFGVLY
                     RFAPLLRGQKDHIRVAGDDFKWRMKLLVGFETDFLLNAQIKYTVDVNNAGNLVDYGYP
                     IPPAIGGYPDYAPNQSNNYKSYYKTIQGVGVIRFGFDHIFKNNLFLGVAMETKFGFND
                     INANEFKVHPKYKKSKNYFIGLNVEFGYNFQRDKMKKAAEKKQTTKAPKYKPEKVKTP
                     GIDLNKRVDEVDKATKKKYKK"
     gene            6697..8199
                     /locus_tag="PLL59_07115"
     CDS             6697..8199
                     /locus_tag="PLL59_07115"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HMY23614.1"
                     /translation="MSNKKNSLSKSTLIRSIQCSKSLYLYKNNFQLRDKPSTTQQQKF
                     DRGHRIGKLAHQYFPGGKDCSPPNPFSYDASIAATKLLIQQQFAVIYEAAFKHNGILV
                     ALDILENKNGKWYAHEVKSSFRISNTYLLDAAIQYYIITKSGIELEGFDIVTINNDYV
                     FDGTLAISSYFKCTSVMKEIMERLPFVEKTIEEAIATLSSDTMPSIDIGAHCNKPYPC
                     DFQGYCWKNVDADSIWYLPGISMQEKSSFIEKGINKIQDIINTDEFNARQKVIIQSFQ
                     KQQVHIDADKLRAFVQPLQYPLYYFDIEAFQPAIPIFPNTKPYERIPFLYSLHYKENA
                     DSPLEHICYISPVGNDDRENFVQHFLLATEKSGQILVFNTLMEKGILFKMATDFPQFK
                     TQILERINRIIDIEVPFKELYYYHPAQQGSFSLKAIGNAMLGIDEFSHSIVKDGEEAM
                     AVYNELFYWENKEEVQSKLEHLKTYCYTDTFVLSKIVDVLAQQVKMQHKI"
     gene            complement(8268..10601)
                     /locus_tag="PLL59_07120"
     CDS             complement(8268..10601)
                     /locus_tag="PLL59_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMY23615.1"
                     /translation="MINFAYKSLLYRSLTTAIFLSCFAVLSAQNLALGTWTAHLPLSN
                     ALSVCQSKDYVYAGTENGVIGVHKESHFIEKYTKVNGLADVFVAQTGYDTSTSTLLIA
                     YKNSDIDLIHEGKIKNLPYLKNAMLAGDKNVYSIFCVNGFAYLGTGFGVLKINLEKQE
                     IAETYIFNDGILTYKVNAVWADDNTIFAATQKGVLSAAISPSINLLNFNNWTQHSIGI
                     PQAEASAITQYDNKIIASISNVLYAFDGVNWSVFFSADANWITRHLNTSNGNLLLAQQ
                     RVSGADVVENRIGKWNGTTFAFFTSANNIAYPLQIIEDENNSIWYADLYRGLVHQEGA
                     NFNAYFPNAPARITSKEMEFLGNTLWVASSNINDGGAPTFNKNGIYACTEYFWENIDI
                     YNTPALDSFFDIAVVQPIAVENKIVFGSQMGILVYDINDKTFDINKYRPNAIDPSKKI
                     FRITGADIDGAANVWLSDAYSNVPLVCRKSGGEYTYFNSGFLNGMLVKDIVVDDFNQI
                     WIAKSDASGGLTMLNYGNDIDDKSDDTYFNFRTGAGFGNLPSNNVICMAKDNDGVLWL
                     GTENGIARIACAGYVVDNACEAEQICIDRKDGSGFCDNLLEDEIINCITVDAANRKWI
                     GTNNGLFLVSADGEKTIHYFNEANSPLLSNYIRALAINPENGDLFIGTGKGICTYRAD
                     ATLTTAETEAPYVYPNPVRENYEGPIAIKGIPNNCNVKIVDVSGNLVYETTANGGQAT
                     WDGKLINGERAATGIYYALCKGTSKKDTAKLKFLLLR"
     gene            10747..14061
                     /gene="ileS"
                     /locus_tag="PLL59_07125"
     CDS             10747..14061
                     /gene="ileS"
                     /locus_tag="PLL59_07125"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HMY23616.1"
                     /translation="MSNTKQYREIKNSTQTQIEQEISLFWQSNSIFEKSIAEREGKKP
                     YVFYEGPPSANGMPGIHHVLARTIKDIFCRYKTIQGYQVKRKGGWDTHGLPVELQVEK
                     LLGITKKDIGTKISVAEYNQKCREEVMKFKDVWDNITQKMGYWVDLNNPYITFDNNYI
                     ESIWWILKELYHKDLLYKGYKIQPYSPAAGTGLSSAELNLPGCYKDVKDTSAVAQFKI
                     SKQSILDSDFLPNKNEDYYFLAWTTTPWTLPSNTALAVGANIEYVLIKTINPYTHTSV
                     QLILAKDLLHKYFQAENENKDFATYQNDGKQLPYQHLATFLGSDLKGIRYEQLLPFEA
                     NKKDQIDGDAFRVITGDFVTTSDGTGIVHIAPSFGADDMKVAAENGIGALTLVDMEGK
                     FIDGVGEFSGRYVKDYKNEENYVSVDIDIAIKLKKENKAFKVEKYEHSYPHCWRTDKP
                     IIYYPLDSWFVRVTAVKDRMVALNKTINWKPESTGSGRFGQWLENLIDWNLSRSRYWG
                     TPLPIWRTEDKTEEICIGSIAELKAEMQKAIDAGFMDNSILDKTIDLHKPYVDDIILI
                     SASGKAMKRESDLIDVWFDSGAMPYAQWHFPFENKDTFAQSFPADFIAEGVDQTRGWF
                     YTLHAIATMLFDNVAYKNVVSNGLVLDKNGNKMSKSKGNTVDPFKTIEKYGADVTRWY
                     MISNSQPWDNLKFDEEGLAETGRKLFGTLNNTYNFFAIYANIDGFTIDKNNTTPLADR
                     AEIDRWVISKLNSLTAEVATAYEEYEPTKATRLIEDFVIEHLSNWYVRLCRRRFWGNE
                     LSQDKKAAFETLHECLRTIAQLMSPVAPFVSDWLWQNLLATNDSVHLSLLQQADDTVI
                     DKALEQRMEWAQEISSLVLSLRKKTKIKVRQPLQKILVPAIDKSFIEQIDKVKQLILS
                     EVNVKEIEYISDTTGIISKKIKPNFKSLGKKLGSHMKAVAEKIQELDNTAIAAFEKNA
                     SIDINSNGETFTILISEVEIISDDIQGWLVASNNGITVALDTHISEDLKNEGFAREIV
                     NKIQTERKESNFEVTDKIILHVQNQLVLNGILNSYKEYICNETLASDILFSDNVSNAK
                     EFDINGEILQLFIQKK"
     gene            14065..15042
                     /locus_tag="PLL59_07130"
     CDS             14065..15042
                     /locus_tag="PLL59_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HMY23617.1"
                     /translation="MAKVTKKAKPVAKKVAAKKPAAKAVKAVATKKAAPKKAVAKSKP
                     APKKAVAKPKAKVVAKPTKKVAAPAKKTVAPAKKAVAKTKPAPKKATTKPVAKKVAAK
                     PAPAKKVAKATPKKVVAKPAPKKTTTKPAVKKAVAKKPIVAKATTPKVVIEKKQAPKA
                     NVKAVAPKPEKIKQNTVKPIHQAAAEKAHQLSATRHQQEAKPVMEVKVVRYSDADLSI
                     FKEVILKKIAKAKEEIDFYTEQIKNASESETQFASIDDGSITSERENMNQIVARMLKL
                     ISHLESALVRIENKSYGICRETGKLIPKERLMVVPHATLSVEGKKMEKK"
     gene            15099..15743
                     /locus_tag="PLL59_07135"
     CDS             15099..15743
                     /locus_tag="PLL59_07135"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HMY23618.1"
                     /translation="MSKKTIAILTIVISLVIDQASKIWVKLTMRSGDEFMYIGNWARI
                     HFVENEGMAFGMSFGAGFGKLMLTVFRIIAVFFITYYLSQQIKDKKSSKGFVFALSLI
                     LVGAVGNIIDSIFYGQIFSHSEGQIATLFPAEGGYAKWFHGRVVDMFYFPIYRGILPE
                     WIPFFGGKFFEFFQFIFNVADACITVGVAIILLFQKQFFKEEATQDIQATSSNP"
     gene            complement(15830..>16220)
                     /locus_tag="PLL59_07140"
     CDS             complement(15830..>16220)
                     /locus_tag="PLL59_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23619.1"
                     /translation="QSGNARINAIEFSNTLNQEAHLNEVYFTNTSSNYEVKFNQIFTV
                     DLDDTTPLNYFIKSNEYKDVQINVYKSKGEYSTKLNKNLNIGDNFIMFDELSANYHGK
                     YIVVITENKPNNKTKKSSNSNITVMND"
BASE COUNT         5758 a         2866 c         2770 g         4826 t
ORIGIN      
        1 tattagcgtt gtagcggcac aagaacaacg cagaggcaat gatccgtttg ctgacatgat
       61 ggaggaaatg gaagaaatgc aacgccaaat gatgcgtcaa tttcagcagc cacaatacag
      121 cgaagcagaa ataaaacagt acttagcaaa aaacttattt gttcgtgtca ttccaacaaa
      181 atcgacattg taccaaggcg aacaaacgac attatcttat aaaatatacg cgcgtgtatc
      241 ttgtaatggt tttatggccg acaaaatgcc gagctacaat ggtttcctta gtgaagagtt
      301 taaactacca gagcaacaag aaccaaaaat agaagtgcta aacggtgtcg aatatcaaac
      361 actcgaagtt aaacgcgtta cattatttcc gcaaaaaaca ggcaagctaa acatcgaacc
      421 attagcactt acaactaatg catacattgg ctatcagccg tatgaataca gctttaaaag
      481 caatacaatt actttagacg taaaaccact gcctgaaaaa aacaaaccac ttacttttag
      541 tggtgctgtt ggaaaatttt ctttcgaagc agattatgat aaaacaaaaa caagtgttgg
      601 cgaaccaatt actttaaaaa tcacttacac aggcactgga aatcttaaat tgattacggc
      661 acctaagtta gaatttcctg aagaatttga gtcgttcgag ccaaaaacca aagacaatta
      721 tgaaaacaat ggcaatgtgg ttagcggctc aaaaacattt gaatatgtat taattccaca
      781 agatggtggc acttttaaac tgcctaaata tgagttcgca tattttgatg ttgacaaagg
      841 tgattacgtg aagtttgtat tgccagaaac agaaatcaca gtgagcggaa aagctaagct
      901 aagcgaaaat gtggccaatt ttttcaaacg agaaaagcaa gataaaccac gctctattta
      961 tgctattaaa aaagtgtatg taaaaaataa atcgtttgcg gcaagcaaca tattttggac
     1021 acttgctatc acgccaatcg tttttcttat aatagcattt gtttttagaa aacgtgatta
     1081 ctcaagcact gaattaattg cacttcgcag aaaaaaagcc aataaaatag cattacaacg
     1141 tttatcgatt gcaaaaaaat tattgcagca aaaacaagaa gaagcattct acaacgaagt
     1201 ggtgcgcgca ttatggcatt atttatcaga caagttatac ctgccacaaa gtgaattgtc
     1261 gaaagaaaac ataagtgaga aattacatct tcgagcaata gaacaaagca agataaacga
     1321 tttacacaac acattagaca catgcgagca ggctttattc tcaccaattg gtcaagaaat
     1381 ggcgatgcaa caaacctaca caaaggctat cgaattaatt atagatttgg aagaacaatt
     1441 aaaaaaagat tcgaaaattt aaatataatc agtagtattc atgagcaaac attctaagta
     1501 catattactc gttttcttct acttgatatt tggatttacc aaagcacaaa acaatatctc
     1561 attatttcaa actgccaata aatactatca aaacaagcaa tacgaagaag cagaaaaaat
     1621 gtatttattg ttgctgaaaa aagagaataa caatgcaaat gcaaactata atcttggcaa
     1681 tacctatttc catttaaaac aatatacgaa tgctatttta tactatgaaa aagcgaaaaa
     1741 aattgaacct gacaataaac aaatccagca caatatcgac ttaaccaaca ataaattatt
     1801 ttcaaaaata gaattcagta aaacattttt tgttgccaaa aaaataaaag gctttgcaca
     1861 tactaaatct gctgacactt ggagcatcta tttgcttgtt tctttgtgga ttggcattgt
     1921 gctcatttgc atacattttt taaaaaacaa gcaaatggca tttaacatag gcgctacaat
     1981 attacttttt gcaatgcttt tcgcttattt tacttatact acatacaaaa gtgagcaact
     2041 ccaagacttc gcaattgtta tgacgaatga tgctacatta aacaaaatgc ccgtagcgaa
     2101 tattggcaac acagacagca ttcaaagtgg tacaaaagtg caaattattg acagtgacaa
     2161 atcttggtta aaaataaaat taccaaacga taaaataggc tggattagca tagataaaat
     2221 tgcacgcatc taaaacgtga cgcctatcgt tattggcaca taatgtttaa tagtaatttc
     2281 tggcgtattc gcataagttt ggtttatacc gccagagccg tttaaacttt gaatatccac
     2341 tttgcgttgt aagtgttgta agcgataacc agcaccaaag tggatagcca attttcgtga
     2401 atttgtgacg tagcgaccgc caatcatgaa ttcgccaaaa gcaccggcat atctgccttt
     2461 gttaatctga ctattaaagt aagaatcaat atagatagcg ccaccaactc gctctgataa
     2521 aaataaatta aaattacctt ctaaaaaata ttctctaaat tctgcaaaaa tgggaaaagt
     2581 gaaataataa aagcgcgaac cattagtacc attaggtcga aagtcaatac ccagtcctgc
     2641 accaataaac aacttcggat tgataagagc gccatgcact gtgcgaaaaa taaaactcgg
     2701 cttctccact tgcaaggtgg tctttttcgt tatatctgga aatttctgga ttaattttcc
     2761 agctacatgt atgccaacgc caacttcatt gatactagaa taacgggtag tttttatatt
     2821 tttaatcgaa tctaaatcca atacaatatc atcttgtgct tgtacgcaaa aaaataaaaa
     2881 accaaaaaac agaaaaaaca gacctcgctt cataatatta ttttacaaca aaattaaaca
     2941 ctaaaatgtt ttattactcc ttaattatta aatttatacg ttattctcaa cacatcgatg
     3001 gtaatacgtt ttacaatttt attttcgctt atctgtaact tcaatatttt gctagcgcaa
     3061 acttttgaat attcctataa ttgcaaacag gcttaccaaa atatttataa attaaaattt
     3121 aacgaagggc aacaatggtt agcacaggaa aaagccgata acccaaaaaa tgtattgact
     3181 tattttatag aaaatcatat tgactttttg aagctataca tcaatgacaa tcgaaagcta
     3241 tatgctgcta ttgaaccaaa aatggaacag cgattagaga aaataaaaaa aggaaatcca
     3301 gtgtctccct attatttata cgcgcaagca gatatacatc tacaatgggc tttttgcaaa
     3361 attaaattcg gcgattattt gagtgctatc tttgaagtaa aaaaatcgtt ttctatgttg
     3421 caagaaaata aaaagagatt tcctgcattc aagccaaaca ttaaaagctt aggattattg
     3481 catacacttt ttggtgcagt gcccgacaaa tacaagttcg gcgcaaaact tttaggttta
     3541 aaaggctcaa ttgatgaagg tatgcaagag ctaaattccg ttttacaaga ctctagtttt
     3601 caatttaaag aagagacgat tatcttatac actttgttgt tattgcattt gcaaaaagat
     3661 aaaaccaatg catggaatat gatagaaaat acgacgattc ctttaggtga taatttatta
     3721 aatcatttta ttgcagcaag tgttgcggag catacaggaa aaaatgataa gatgattagc
     3781 atattagcca atagacctaa tagtgccgcg tattatgcat ttccatatct tgatttttta
     3841 tatggctttg ccaaattaaa cagacttgat gctgatgctg atgtatattt aaaaaaattt
     3901 atcactacaa ataaaggtag agcttaccaa aaggaagcgc tcagaaaact agcttggttt
     3961 tatttgatta atgacaatcc gacaggatat aaaaagtata tgcaacaaat tttgacgatt
     4021 agcgaagcac ccagcgacga agataagtcg gcacaaaaag aagcagagca aaattttatt
     4081 ccaaataaag aattattgaa agcacgtgtt ttaagtgatg gcgcctattt caacaaagca
     4141 ctagcaacaa taaacgccat tgcaccagaa aaacttacac gaacacgaga tatcattgag
     4201 tattactata ggaaagcgag gatttatgac gagctcaaac aaaatacaga agcagtaaag
     4261 tactatctta tttctataca aaaaggcgaa aattacaagt actattttgc agcaaatgca
     4321 tgcataaaac taggtgctat ttttgaagaa cagcataaaa aatcgaatgc tattcaatat
     4381 tataaaaaag cgatacagct cgacaaagat gagtatagca acagcataga tgcggaagca
     4441 aaagctggtt taaatcgatt aggcaattaa tttatgttct ttattttcta gtttcaatgt
     4501 tgttttttca atttccgata aaataatatc gtatttttat gcacgctatg gtcagcattc
     4561 aaaaagatat ttctttatta ccctataata ctttcggcat tgatgtaaat gcaagtaatt
     4621 tttgtcgtgt tagcagcaaa gaagcgctta tttcacttat taatcaagct attttcccca
     4681 tatcagattg gttgattatt ggtggtggta gtaatctttt attttgtaaa aatatggaag
     4741 gcttagtggt aaaaaatgaa atcattggca ttgagctcat caaagaaaat gaaacacatg
     4801 tttggctaaa aagctattca ggcacgatat ggcacgaact cgttatgtat tgtgtggaga
     4861 ataacttggg tggcatagaa aatctagctt taattccagg caccgtaggt gctgcgccga
     4921 tgcaaaacat tggtgcttat ggtgtagaaa tcaaagatac atttgtagaa gcaacagcta
     4981 tcgaattaag cactggcgac acctatattt ttggcaaaga cgcatgtaat tttggctatc
     5041 gcgaaagcat ctttaagaaa gaagccaaag gcaaatattt tatttacgat gttactttta
     5101 aactctcaaa gtcgccgcaa ttaaatatgg cctatggcga tattcagaaa ataattaaag
     5161 aaaaaaacat cgccacacca agcattaaag acgtggctga tgcagttatt caaattcgtg
     5221 aaagcaagtt accaaatcca aaagacatag gaaatgcagg tagttttttt aaaaatcctg
     5281 aaatcagcat agaacaattt tctttattgc aacaacaatt tccaaatatg ccacattaca
     5341 gcttgagcaa tggaaaagaa aaaattcctg ctgcatggtt aatcgagcaa gctggctgga
     5401 aaggaaagca aattggaaac acaggcaacc atgcaaaaca agctttagtt attgtaaatt
     5461 atggtcatgc cactggtgct gaaatatggc aacacgcact tcttgtgcga gatgatgtgc
     5521 agcaaaaatt tggtattata ttagaagctg aagtcaatat tatcgcataa aaaaaggcca
     5581 ttgttgccaa tgacctttct ttcttatttt ttgtattttt tcttggttgc tttatctact
     5641 tcatcaacgc gtttattcaa gtctatacct ggcgttttta ctttttctgg tttgtatttt
     5701 ggtgctttag tcgtttgttt tttctctgct gcttttttca ttttatcgcg ttgaaaattg
     5761 tatccaaatt cgacattcaa accaatgaaa tagtttttag attttttata tttcggatgt
     5821 actttaaatt cgttggcatt aatatcatta aagccaaatt tcgtttccat cgcaacgcct
     5881 aaaaataaat tgtttttaaa aatatggtcg aagccaaaac gaattacacc tacaccttgt
     5941 atggttttat agtacgattt gtaattattg ctttggttgg gtgcatagtc aggataaccg
     6001 ccaattgctg gtggaattgg atatccatag tccactaaat tacctgcatt attgacatca
     6061 accgtatatt ttatttgcgc gtttaacaag aaatctgttt caaagccgac taataatttc
     6121 attctccact taaagtcatc accagcaacg cgaatatggt ctttttggcc acgcaacaat
     6181 ggtgcgaagc gatacaacac accaaaaccc atgtatttaa aatcaacaga ttttcggtga
     6241 gtgcctatta atgaagggta ctttttccac ttaaattcat ctttataatt ttgaccttca
     6301 tcgcaaaaat tggcgaatgc aactataccg tgttgctctt taaaattata acctccttga
     6361 ataaatacat ggtagctaaa ctttggtttg tagtctaact ctttgctatt ttctgctaaa
     6421 aaataagtgt tttgtgccaa catatatgac aagcctggtg tagcaccaat actgagtgta
     6481 ccgccttttt gtgccattaa cacattacag caaaaaatag atagaaaaaa aagtgtaaaa
     6541 tttcttagtt tcatctcaaa attattttat tcgttttaca actccatttt acaaagtata
     6601 ggcaatataa aattgcggaa taagcgcgaa tatttttatt ttacttcgta acggacatca
     6661 aagataataa aacataagca tcttttagtt aatataatga gcaacaaaaa aaattcactt
     6721 agcaaatcaa cacttatacg cagtatccaa tgctcgaaat cattatatct ttataaaaat
     6781 aattttcaac taagagacaa acccagcacc acacagcaac aaaaatttga cagaggtcat
     6841 cgcattggca agcttgcaca tcaatatttt cctggtggca aagattgttc tccgccaaat
     6901 ccattctcgt atgatgcatc aattgcggca acaaaactct taattcaaca gcaatttgca
     6961 gttatttatg aagctgcatt taagcataat ggcatcttgg tagccttaga tattttagaa
     7021 aataaaaatg ggaaatggta tgcacacgaa gtgaaaagct cgtttcgaat ttctaacacc
     7081 tatttattag atgcagccat acagtattac atcattacta aaagtggcat cgagctcgaa
     7141 ggttttgaca tcgttactat caataacgac tatgtttttg atggcacatt agctatttct
     7201 tcctatttca aatgcacttc tgtaatgaaa gaaattatgg aaagacttcc atttgtagaa
     7261 aaaacaatag aagaggcaat cgcaacatta agtagcgaca cgatgccaag tatcgacatt
     7321 ggtgcgcatt gcaacaaacc atatccttgc gattttcaag gttattgctg gaaaaatgtc
     7381 gatgccgatt ctatttggta tttgcctgga atttctatgc aggaaaaaag tagttttatc
     7441 gaaaaaggca tcaataaaat tcaagacata ataaatacag atgaatttaa tgcgcgccaa
     7501 aaagtaatca tccaatcttt tcaaaaacag caagtacata ttgatgcaga caaattacgt
     7561 gcgtttgttc agcctttaca atatcctttg tattattttg acattgaagc attccagcct
     7621 gctattccta tatttcccaa caccaaacca tacgaaagaa taccattttt atattccttg
     7681 cattacaaag aaaatgctga ttctccatta gaacacattt gctatatttc tccagtaggc
     7741 aatgacgata gagaaaattt tgttcaacat tttttattag ctactgaaaa atcaggacag
     7801 attttagtat ttaacacgct catggaaaaa ggtattttat tcaaaatggc aacagatttt
     7861 cctcagttta aaacacaaat tttagaacgc atcaatcgca tcatagatat cgaagtgccg
     7921 tttaaagaat tatattatta tcatccagca cagcaaggca gtttctcatt aaaagccatt
     7981 ggaaatgcta tgcttggcat cgatgaattt tctcatagca tcgtgaaaga tggcgaggaa
     8041 gctatggctg tgtataacga gcttttttat tgggaaaata aagaagaagt acaatcaaaa
     8101 ttagaacact taaagacata ttgctatacg gatacttttg tcttgtctaa aattgttgat
     8161 gtattagcgc agcaagttaa aatgcagcat aaaatttagc acattttatg aaataatatt
     8221 tctcgtttcg tagtaaaaat cattgtttat cctaataaaa atttacctta tcgtagcaat
     8281 aaaaatttca actttgcagt gtcttttttg cttgtgcctt tacataatgc ataataaata
     8341 cctgtagcag cgcgctcacc atttatcagc ttgccgtccc aagtggcttg tccgccattg
     8401 gctgtagttt cataaaccag attaccgcta acatctacaa tttttacatt gcaattatta
     8461 ggaatacctt tgatagcaat tggtccttca tagttttcgc gtactggatt tggataaaca
     8521 taaggtgctt ctgtttcagc tgttgtcaat gtagcatctg ctctgtaagt acaaatacct
     8581 tttcctgtgc caataaagag atcgccattt tcaggattta tggctaatgc acgtatgtaa
     8641 tttgacagta aaggcgaatt ggcttcattg aaataatgta tcgttttttc gccatctgca
     8701 ctcactaaaa acaggccatt attagtgcct atccattttc tgtttgctgc atcaacggta
     8761 atacaattaa taatttcgtc ttccaataaa ttatcgcaaa agccactacc atcttttctg
     8821 tctatacaaa tctgttccgc ttcacatgca ttatccacta catatccggc acatgcaatt
     8881 ctagcaatgc cattttctgt gcccaaccaa agtacgccat cattatcctt tgccatgcaa
     8941 atcacattat tcgatggcaa atttccgaat ccagcgccag ttctaaaatt aaaataagta
     9001 tcgtcagatt tatcatcaat atcattgcca taattcaaca ttgtcaggcc tccactcgca
     9061 tcgcttttag caatccaaat ttggttgaaa tcatctacaa caatatcttt tactaacata
     9121 ccattcaaaa atcctgaatt aaaataggtg tattctcctc cagatttccg acagacaagt
     9181 ggcacattcg aatacgcatc agacagccac acattagcag caccatcaat atccgcccca
     9241 gtaatcctaa atattttctt tgatggatcg atagcattgg gtctgtattt attgatgtcg
     9301 aatgttttgt catttatatc atacaccaag atgcccattt gtgagccaaa cacgatttta
     9361 ttttcaacgg caataggttg tactacggct atgtcaaaaa aagaatctaa cgcaggtgtg
     9421 ttgtatatgt ctatgttttc ccaaaagtat tctgtacaag cataaatgcc atttttatta
     9481 aaagtaggcg cgccaccatc atttatattt gacgatgcca cccataaagt atttcctaaa
     9541 aattccattt cttttgaagt aattcgtgct ggcgcatttg ggaaatacgc attgaaatta
     9601 gcaccttctt gatgcaccaa acctctatat aaatcggcat accaaatact attattttca
     9661 tcttcaataa tctgcaaagg ataggcaata ttattagcac tcgtgaaaaa agcaaatgtt
     9721 gttccattcc attttccgat tctattttcc actacgtctg caccacttac tcgttgctgg
     9781 gcaagcagta aatttccgtt tgacgtattc aaatggcgtg ttatccaatt ggcatcagca
     9841 ctaaaaaaga cagaccaatt gacgccatca aatgcatata aaacattgga aatacttgca
     9901 ataattttat tgtcatattg cgtgattgca cttgcttctg cctgtggtat tccgattgaa
     9961 tgctgtgtcc aattattaaa attcaataaa tttatcgatg gtgaaatggc agcactcaac
    10021 acaccttttt gcgtagcagc aaagatggta ttatcatcag cccaaactgc attgacttta
    10081 taggttaaaa tgccatcatt aaaaatatag gtctctgcaa tttcttgctt ttctaagtta
    10141 atttttaaga caccaaatcc tgttcctaaa tatgcaaatc cgttaacaca aaaaatggaa
    10201 tagacattct tatcacctgc aagcattgca tttttcaaat agggcaggtt ttttatcttt
    10261 ccttcatgta taaggtcgat gtccgaattt ttgtaggcaa tcagcaaggt agaagtgctc
    10321 gtatcatatc ctgtttgtgc cacaaataca tcagccaatc cgtttacttt tgtgtatttt
    10381 tctataaaat gactttcctt atgcacgcca ataacaccat tttccgtacc tgcatataca
    10441 taatccttgc tttggcacac agatagagca ttgcttaatg gcaaatgtgc agtccaggta
    10501 cccaaagcta gattttgcgc cgacaaaaca gcaaagcaag acaagaaaat tgccgttgtc
    10561 aaacttctgt atagcaagct tttgtatgca aaatttatca ttttgaacta ttcaagtgat
    10621 ttaaaggtaa atttttacca aatctataca ttttatgcga gattgataga atatattaac
    10681 gtacaaaacc aataatttat atagctttta gtatctttgc gcaaattttt tttgatacgg
    10741 aaagacatgt cgaatacaaa gcaatacaga gaaataaaaa acagcacaca aactcagata
    10801 gagcaagaga ttagcctatt ttggcaatca aattctattt ttgaaaaaag cattgctgaa
    10861 cgcgaaggca aaaaacctta tgtgttttat gaaggaccgc cgagtgctaa tggtatgcct
    10921 ggaattcatc atgtattagc gcgaaccata aaagatattt tttgtcgcta taagacaata
    10981 caaggctatc aggtaaaacg taagggtggc tgggacacgc atggtttgcc ggtagaactg
    11041 caagtagaaa aattattagg aatcacaaaa aaagacatcg gaacaaagat ttccgttgca
    11101 gaatacaatc aaaaatgccg agaagaagtg atgaagttca aagatgtttg ggacaacatt
    11161 acacaaaaaa tgggctattg ggtggactta aataatccat acattacatt tgacaacaac
    11221 tacatcgaaa gtatttggtg gatattaaaa gaactatacc ataaggattt attgtacaaa
    11281 gggtataaaa ttcaaccata ttctcctgct gctggcacgg gtttgagttc tgctgaatta
    11341 aacttgcctg gttgctataa agatgtgaaa gacacgagtg ctgttgcgca gttcaaaatc
    11401 agcaaacaat ccatactaga tagtgatttt ttaccaaaca aaaatgaaga ttattatttc
    11461 ttagcttgga cgactactcc atggacatta ccatctaaca ctgcattggc tgttggtgca
    11521 aatattgaat atgtattgat taaaacaatc aatccttata cacacacaag cgttcaatta
    11581 attttagcaa aagatttatt acataaatat tttcaagcag aaaatgaaaa taaagatttt
    11641 gctacttacc aaaatgatgg caagcaacta ccttatcagc atttagccac ctttttgggc
    11701 agcgatttga aaggcatcag atacgagcaa ttattgcctt ttgaggccaa caaaaaagat
    11761 cagattgatg gcgatgcttt tcgtgtcatc acgggcgatt ttgttaccac atcagacgga
    11821 acaggcatcg tgcacattgc accaagtttt ggtgctgacg acatgaaagt agcagctgaa
    11881 aatggtattg gcgctttgac attagtagat atggaaggta agtttatcga tggcgttggc
    11941 gaattttctg gaagatatgt aaaggattac aaaaacgaag aaaactatgt ttctgtagat
    12001 atcgatattg ctattaaact aaagaaagaa aataaagcat ttaaggtaga aaaatacgaa
    12061 cactcgtatc cgcattgctg gcgaacagat aaacctatta tctattatcc tttagactct
    12121 tggtttgtac gtgttactgc tgtcaaagat cgcatggtgg cattaaacaa aaccatcaac
    12181 tggaaaccag aaagtacggg cagcggtcgt tttggtcaat ggttagaaaa tttaatcgat
    12241 tggaatttat cgagaagcag atattgggga actccattgc caatttggcg aacggaagat
    12301 aaaacagaag aaatttgcat tggctctatt gctgagctaa aagctgaaat gcaaaaggca
    12361 atagatgcag gattcatgga taatagcatc ttagataaaa ccattgattt gcataaacct
    12421 tatgttgacg atatcatctt gatatcagcc agtggcaaag caatgaaacg cgagagtgat
    12481 ttgattgatg tgtggtttga tagtggtgca atgccttatg cgcaatggca tttcccattc
    12541 gaaaataaag atacttttgc gcaatcattt ccagcagatt ttattgcaga aggtgtcgat
    12601 caaacgcgtg gttggtttta tacattacat gccattgcta caatgttatt cgacaatgta
    12661 gcttataaaa atgtcgtgtc aaatggtttg gttttagata aaaatggcaa taaaatgtct
    12721 aagtcaaaag gcaatacggt tgatccattt aaaacaatag aaaaatacgg cgctgatgta
    12781 acacgctggt atatgatttc caattcacaa ccttgggaca atcttaaatt tgacgaagaa
    12841 ggcttagctg aaaccggcag aaaattgttt ggaacattaa acaatacgta taattttttc
    12901 gctatttatg ctaatataga tggttttacc attgacaaaa acaacaccac accattagcc
    12961 gatagagcag aaatagaccg ttgggtaatt tctaaattga actcacttac agcagaagta
    13021 gccacagcat acgaagagta tgaacctaca aaagcaaccc gattaattga agatttcgtg
    13081 attgaacatc tgtctaattg gtacgtgcgt ttatgccgtc gcagattttg gggaaatgaa
    13141 ttatcgcaag ataaaaaagc ggcatttgaa acactacatg aatgcttgcg tacaatagca
    13201 caattaatga gcccggtggc accatttgtc agtgattggt tatggcaaaa cttgctcgca
    13261 acaaatgatt ccgtacatct atcattgcta caacaagcag atgacactgt aatagacaaa
    13321 gccttagaac aacgtatgga atgggcgcaa gaaatttcgt cattagtact ttctttaaga
    13381 aaaaagacga aaataaaagt tcgtcagcca ttacaaaaaa ttttagtgcc agcaattgac
    13441 aaatcattca tcgagcaaat cgacaaagta aagcagttga ttctctcaga agtaaatgtc
    13501 aaagaaatcg aatatatcag cgacacgact ggtattatat ccaaaaagat aaaacctaat
    13561 ttcaaaagtt taggtaaaaa attaggcagt cacatgaaag ctgtggcaga aaaaatacaa
    13621 gaattagaca atactgccat tgcagcattc gagaaaaatg ctagcataga tatcaattct
    13681 aatggtgaaa catttactat tttaatcagt gaagtagaaa tcatatctga cgatatccaa
    13741 ggctggctgg tggcttccaa caatggaata actgtagctt tggatactca tatttcagaa
    13801 gatttaaaaa atgaaggttt cgccagagag attgtcaaca agatacaaac cgagcgaaaa
    13861 gagtccaact ttgaagttac tgacaaaatc attttgcatg tgcaaaatca attggtactc
    13921 aatggtattt tgaatagtta taaagaatat atttgcaacg aaactttagc aagcgacatc
    13981 ttattttctg ataatgtatc gaatgcaaaa gaatttgata ttaatggtga gatattacaa
    14041 ttgttcattc aaaaaaagta gaagatggca aaagtgacaa agaaagcgaa acctgtagcg
    14101 aaaaaagtag ctgcaaaaaa accagctgca aaagcagtaa aagcagtagc taccaaaaaa
    14161 gcagcaccta agaaagctgt agcaaaatca aaaccagctc ctaaaaaagc agttgcaaaa
    14221 ccgaaggcaa aagtagtagc aaaacctaca aaaaaagttg cagcacctgc aaaaaagaca
    14281 gtagcacctg caaaaaaagc agtagcgaaa accaagcctg ctccgaaaaa agcaacaaca
    14341 aaaccagtag caaagaaagt agctgcaaag ccagctcctg ctaaaaaagt agctaaggca
    14401 acaccaaaga aagtagtagc caaaccagcg ccgaaaaaaa cgacaacgaa accagcagta
    14461 aagaaagccg tagcgaagaa acctattgtc gcaaaagcta caacaccaaa agttgtaatc
    14521 gaaaagaaac aagcacctaa agctaacgta aaagcagttg caccaaaacc tgaaaaaata
    14581 aaacaaaata ctgttaaacc gatacaccaa gctgcggccg aaaaagcaca tcaattatca
    14641 gcaacaagac atcagcaaga ggcaaaacca gtgatggaag tgaaagtagt gcgatacagt
    14701 gatgcagact tatctatttt taaagaagtc attttaaaaa agattgctaa agcaaaagaa
    14761 gaaattgatt tctatacaga acaaataaaa aatgcatctg aatcagaaac gcagtttgcc
    14821 agcatcgacg atggctctat cacctctgaa cgtgagaaca tgaatcaaat agtagcacgc
    14881 atgctcaaat taatttctca tttagaaagt gcattagtac gtattgagaa taaatcgtat
    14941 ggcatttgtc gcgaaaccgg aaagctcatt ccaaaagaac gtttgatggt agtgccacat
    15001 gctacattga gtgttgaagg gaaaaaaatg gaaaaaaaat aacaacaaca aaaatcccgc
    15061 aactgcggga ttttttttaa taatcaacaa atacattcat gagtaaaaaa acgattgcca
    15121 tacttactat cgttatatct ttagttattg atcaggcttc gaagatttgg gtaaaattaa
    15181 caatgcgcag cggcgacgaa tttatgtaca ttggcaattg ggcacgcata cactttgtag
    15241 aaaacgaagg tatggcattt ggcatgagct tcggtgcagg atttggcaaa ctgatgctta
    15301 ctgtgtttag aattattgct gtgtttttta ttacttatta tttatctcaa caaatcaaag
    15361 acaaaaaatc atcgaaagga tttgtatttg cactttcctt aattttagtc ggcgccgtag
    15421 gcaatatcat agacagcatt ttttatggcc aaatattctc gcatagcgaa ggacaaattg
    15481 ccacattatt tcctgctgaa ggtggttatg caaaatggtt tcacggcaga gtagtggaca
    15541 tgttctattt tcctatatac agaggaattt taccggaatg gattccattt tttggcggaa
    15601 aattctttga gttttttcaa tttattttta atgtcgctga tgcatgtata acagtcggtg
    15661 tagctattat tcttctcttt caaaagcaat ttttcaaaga agaagccaca caagatatac
    15721 aagcaacatc atcaaatcca taaaaaaaga tagaccttac tccgcacctt ttacagcttg
    15781 gaataaggtc ttgtccccca actggactac ttaaattttc atttttgaat taatcattca
    15841 tgactgttat gttggagttg gaggatttct tggttttgtt attaggtttg ttttctgtga
    15901 tgactactat atattttccg tgataatttg ctgataattc atcaaacata ataaaattat
    15961 ctccaatatt taaattctta tttaattttg ttgaatattc tccttttgat ttatatacat
    16021 ttatttgaac atctttatac tcatttgatt ttatgaaata attcagtggt gttgtatcat
    16081 ctaaatctac agtaaaaatc tgattaaatt ttacttcata attgctcgaa gtatttgtaa
    16141 aataaacttc attcaaatgt gcctcttggt tcaatgtatt agaaaattca atcgcattta
    16201 tacgcgcatt tccactttgc
//
LOCUS       DAOQQV010000056        16206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1126_length_16206_cov_6.408953, whole genome shotgun sequence.
ACCESSION   DAOQQV010000056 DAOQQV010000000
VERSION     DAOQQV010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16206
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1126_length_16206_cov_6.408953"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="pgk"
                     /locus_tag="PLL59_07145"
     CDS             <1..435
                     /gene="pgk"
                     /locus_tag="PLL59_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMY23620.1"
                     /translation="RRAKEKGVNILLPKDSVIADKFDNAANTQITINTAIPEGWMGLD
                     IAHEAIQEFETTIMTSKTILWNGPMGVFEMDNFQNGTKSIAVAVAHATAEGAFSLVGG
                     GDSVAAVNKFELAEKVSYVSTGGGALLEFFEGKTLPGVAALL"
     gene            complement(499..714)
                     /locus_tag="PLL59_07150"
     CDS             complement(499..714)
                     /locus_tag="PLL59_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23621.1"
                     /translation="MKKVFFVCMAVGLLATSCDWFKSKPATPEITDVTIETPDSTVST
                     LQDSTTQIKDSSVAVVEKKEASKEDKK"
     gene            complement(830..1579)
                     /locus_tag="PLL59_07155"
     CDS             complement(830..1579)
                     /locus_tag="PLL59_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMY23622.1"
                     /translation="MSIKTTAIYLLLAVIAEILGTIGGFGSSTFFVPLSNYFFDFQTV
                     LGITAVFHVFSNISKIALFKNGLNKQIILRIGVPAVIFVIIGAVASKYINIDYLQLAL
                     GVFLLIISIVFYIKKEIRVEPSQKNGFIGGSISGFVAGLLGTGGAIRGVTMTAFNVEK
                     SVFVATSAAIDLAVDFSRSIVYFVNGYIHKDIIVIIPFLVIVSIVGSYIGKIILDKIP
                     PEKFRVLVLFLIFLVGITLILKHFGIDYFAE"
     gene            complement(1583..1996)
                     /locus_tag="PLL59_07160"
     CDS             complement(1583..1996)
                     /locus_tag="PLL59_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMY23623.1"
                     /translation="MKKMAVIRKANFSAAHKLWNEKWSTEKNKAVFGLCANENFHGHN
                     YNLELKVVGEIDAETGYVIDLKIIKKIIKEEIEDRFDHKNLNLDTKEFAQLNPTAENI
                     AIVIYDIIRQQLDEKYALYIKLWETEKNCVEYPYP"
     gene            2128..3246
                     /locus_tag="PLL59_07165"
     CDS             2128..3246
                     /locus_tag="PLL59_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23624.1"
                     /translation="MLFKDVIGQTEIKQHLLDIAHSGTIPHAQLFLSQPGAGGLALAL
                     AFTQYIICENKQAEDSCGTCPACVKAQKLVHPDIHFTYPIIAKKPSPHPSLSTDYIQE
                     WRKEIIKNAYINELEWLQAIDAENKQGNISSDEARNIIKGLNLTAFEANYKIHIIWMA
                     EALSKEGNILLKLLEEPPANTVLILIAENQEEILPTILSRCQILKINRINDDAIATAL
                     LSKGVPQSNTQHIAYLADGNFNEAIKLIDSETGANTRLLTQWLDYSINLKTGELLKWT
                     EENAKLGRENLKSFLEYTTHIFRESLMLMHIPNYIPRLANEENNLALRINKFMNHEKL
                     AKLLELINKKHYEIERNVSSKMVLMDMSLQMRKIIRGK"
     gene            3395..4186
                     /locus_tag="PLL59_07170"
     CDS             3395..4186
                     /locus_tag="PLL59_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23625.1"
                     /translation="MSKVVEIGQTDIKYKKFDNLDGPIYTEDKSNFVSVIFENGKIEA
                     IVSSPLTANDRHFGKARFTPLQPKYKNSIDFDFFSFYTADVSLQYTRYLKNEHFAITV
                     PIRVGWYYNGFNGMYLLMEEDRLYFQENNSQSYYYSYPADKPKGFKLLSGINVKYFFK
                     EAVKFRGYIAPEFAFGVHKETNKYYFYDSNSSNTESKELSHTAGLLGILGVFGFQATP
                     SSKIFIKLEMAGGYGGLISKNIYDHHTFVGRATFALGVNFKKKQD"
     gene            4391..5698
                     /gene="ricT"
                     /locus_tag="PLL59_07175"
     CDS             4391..5698
                     /gene="ricT"
                     /locus_tag="PLL59_07175"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HMY23626.1"
                     /translation="MSCNSCGTSGSTPGGCRNNGHCATGGCSKLEVHDWLSDINADPY
                     AKYNVHEVSFKNGSRKGFYRNDKRFDVFTGDYVIVEAAQGYDLGQVTLSGELVRLQMK
                     RKRVKDAEENLPTIMRIAKDNELDLMEQARAKEKQTMLKARVLARNLGLDMKLGDVQY
                     QADGKKATFYYTADGRVDFRELIKVLATEFKVKIEMRQIGARQEAGIVGGIGSCGREL
                     CCSTWLTSFKSVNTTAARYQNLSINQTKLSGQCGRLKCCLNYELDTYLDALRFVPEKA
                     EYLQLQSGDAKLMKTDILKGLMYYTVKGSGVFYPITLENVKLVLEMNANGKKPAEITD
                     LAEVEEKSEEESIEFADVVGQISLASLDRKNKKHHPKNKPNTLKQQRDNTTQRQMPTQ
                     EQQANRHQSNKQHGRSNRNQQNRPNKSANQSDNKNQNKDNKQD"
     gene            complement(5695..6537)
                     /locus_tag="PLL59_07180"
     CDS             complement(5695..6537)
                     /locus_tag="PLL59_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23627.1"
                     /translation="MQKIEFIIKGINEKPISIDIRYIENKQRKPIIIFSHGFKGFKNW
                     GHFDIIADYFAQNGFLFVKYNFSHNGTTAQQLCDFVNLEAFGQNNFSKEINDLSFVID
                     YIYNNANDFEGNNNEIYLIGHSRGGGISILKAANELRIKKLCTWASIQDISYFFQNMD
                     IEKWKIENVAYTFNSRTQQNMPLYYQLYEDYLKNKNELDITKAATSLSIPWLIIHGTN
                     DNSVSPVMANALHQINPKSEILLIEDADHTFGGKHPWHSENLPIASKILVEKTLLFFT
                     EKYV"
     gene            6601..7575
                     /locus_tag="PLL59_07185"
     CDS             6601..7575
                     /locus_tag="PLL59_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMY23628.1"
                     /translation="MSNTVDNLKAPIEQELEIFEQKFREVMRSHVSLLDKVNYYIYQR
                     KGKQVRPMFIFLAAKMCGEVTESTYVGASLVELLHTATLVHDDVVDDAYERRGFFSIN
                     ALWKNKIAVLVGDYFLSKGLLLALDTKNYNLLHITSTAVKRMSEGELLQMEKARKLDI
                     DESVYLEIITNKTASLIAAACEVGAASTTTDTALIEKMRLIGEYIGIAFQIKDDLFDY
                     GEADIGKPRGIDIKERKMTLPLIYTLNNASKEEKKWIINTVKNHNENKQRVQELIQFI
                     HKKGGIQYTEEKMHEYKLRAFEIIKTIPESPAKTGLIDLVNYITERNY"
     gene            7577..8587
                     /locus_tag="PLL59_07190"
     CDS             7577..8587
                     /locus_tag="PLL59_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMY23629.1"
                     /translation="MSIAQQAPNNKEKFFVQSLTLVVGLALLLVKFFAYFTTNSNAIL
                     TDALESIVNIVAGSFGLYSLYLAAKPNDEDHPYGHGKVEFISASIEGSLIAATGIAMI
                     IKSSYNFFVPTSLDNLDVGLMLIVIAGIINFILGKIAVAYGKKNSSLTLIASGEHLKV
                     DAYTSVGILLGLGIVYFTDFLWLDNVVAILMGIFIVYNGIKILKKSVAGIMDEADFFL
                     LEKVVVQLNDNRRMNWIDIHNLRIIKFGEKIHIDCHTTIPWYFNANEVHEELKLVESN
                     IKQIIPNNLETFIHADPCLPKSCRSCLKTDCKVRQFPFERKIEWTLENVMKNEKHFLE
                     KT"
     gene            8631..9113
                     /locus_tag="PLL59_07195"
     CDS             8631..9113
                     /locus_tag="PLL59_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HMY23630.1"
                     /translation="MKKKTVKYQIREDEIIHKVAEKEIAYYRASSVPALLNDDTHFLI
                     DKTRKGISMHQIQQQMNKYDLTLKEMANVLNVSERTLQRYETHDLLGKDASERALHLQ
                     RLYERGTEVFGSLSVFCEWMKTPVLLYNHELPISYLDTIFGFELLEQELGRIEHGIFA
                     "
     gene            9119..9577
                     /locus_tag="PLL59_07200"
     CDS             9119..9577
                     /locus_tag="PLL59_07200"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HMY23631.1"
                     /translation="MIVYRICNKLYANDLQGIGAKMYGGRWNNVGNAVVYTSSSRALA
                     ALEVLVHLPSTTLKKLDFTVVSIEIPENSVEEIFYDDIKRDFQINRFSAFFKNIGDNW
                     LRENKSLLLKVPSVVIHEEMNYLINPNHKDFNKVKLTETKLFRFDDRLAK"
     gene            complement(9581..10309)
                     /gene="lptB"
                     /locus_tag="PLL59_07205"
     CDS             complement(9581..10309)
                     /gene="lptB"
                     /locus_tag="PLL59_07205"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HMY23632.1"
                     /translation="MVLRAENLVKTYSNRKVVDNVSIEVNQGECVGLLGPNGAGKTTT
                     FYITVGLVTPNQGKVFLDNKDISSVAMYKRAKLGVGYLPQENSVFRTLSVEDNIKAIL
                     EFSDLSKTAQKEKLESLLEEFGLKHVRKTKGKLLSGGERRRTEIARALASDPKFILLD
                     EPFAGIDPIAVEDIQHIVEKLKFKNIGILITDHNVHETLSITDRAYLLFEGKIIKQGT
                     ADDLASDEQVRKVYLGQNFELRKK"
     gene            complement(10285..12006)
                     /gene="recJ"
                     /locus_tag="PLL59_07210"
     CDS             complement(10285..12006)
                     /gene="recJ"
                     /locus_tag="PLL59_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HMY23633.1"
                     /translation="MLQNKRWTYLEADENAVQELQKQLNIHPTLCKLLVLRNIKTFDE
                     AKDFFRPSLEKLHDPFLMKDMDKAIERIELAILKNEKILIYGDYDVDGTTAVSLVYSF
                     FKDIYPNLEYYIPDRYTEGYGISLQGIDYAAENNVQLIIALDCGIKAHDKINYANEKG
                     IDFIICDHHLPGDTIPSAVAILDPKREDCTYPFKELSGCGIGFKLIQAYTLKNQLDIE
                     RVYEHLDLVAVSIASDLVPITDENRILAKFGVDKVNTNTRAGFKALMSELKLTRILDI
                     NDLVFIIGPRINAAGRIAHGSEAVTLLIEKDYDVAVEKTKKVQQNNSDRKTLDKDITE
                     EAFAMIDTNDLLIQKKSTVLYQEHWHKGVIGIVASRLIEKYHRPTIILASSNGKAVGS
                     GRSVPGFDLHDAIDACSEHLVQFGGHKYAAGLTIEIDKIDTFIEAFEKTVSERILDEQ
                     LIPQIEIDAIIELNDINEKFFNIVEQMGPFGPGNMRPNFVTKDVYDTGYSKVLNGNHL
                     KLNLLKDGQNPRNGVGFGMGDYMKILENNETVDICFQMYANIWNGQTKIEFRLKDLKA
                     WYYAQKI"
     gene            12217..12528
                     /locus_tag="PLL59_07215"
     CDS             12217..12528
                     /locus_tag="PLL59_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23634.1"
                     /translation="MKKIIYTTLGVASVANEKFKELLEDLIQNNQFTEDEGKRIVDGF
                     FFDLRQQLDLVQGNVQSKLDDIMKKFGVSDFYSLKQEFENYVQDVKSNPTLLLKLPSK
                     K"
     gene            complement(12672..14201)
                     /gene="dnaB"
                     /locus_tag="PLL59_07220"
     CDS             complement(12672..14201)
                     /gene="dnaB"
                     /locus_tag="PLL59_07220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HMY23635.1"
                     /translation="MEEQTSNFTKKTTRFTRNKTEDMSNMLYGKVPPQARELEEAVLG
                     ALLIEKGAIAEVNDVLKPESFYVDAHATIFRSIQSLFGKSHPIDLLTVTEDLRKSGKL
                     EEVGGAFYISELTNKIASSANVEFHARIIVQKFIQRELIRISSDIIRDAFEDTTDVFV
                     LLDGAEKKIYEIADKNISRSVQNIGQLVSKSITDIDSLQSREDGLLENSVPSGYVELD
                     RMTSGWKATDLVIVAARPSMGKTAFVLNIARNAAVVHNMPVAIFSLEMGAVQLAKRLI
                     SLECEIDAQKVANGRMSENEYAILRDKVERLSSSPIYINDQPAINVYELRAQCRRLQN
                     AHGIKMVIIDYLQLMSGGGDKGMNREQEISNISRSLKGLAKELNIPVIALSQLNRSVE
                     TRGGDKKPQLSDLRESGSIEQDADMVMFLYRPEYYKLDEAANGAHLKGMSEIIIAKHR
                     NGPTGSVELRFNKHFGRFYDAGGLYDEMQEYSNSYKTVPSKGNFMKDEEGKKTDDFDL
                     F"
     gene            complement(14487..15674)
                     /locus_tag="PLL59_07225"
     CDS             complement(14487..15674)
                     /locus_tag="PLL59_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY23636.1"
                     /translation="MANRYASSFRGFDYLNLDAQLTDEQKLIRDSVRAWVNTAVIPII
                     EDCAEKNTFPKHLIKELGEIGAFGPHIPTQYGGGGLDAIAYGLIMQELERGDSGIRSC
                     ASVQSSLVMYPIWKFGSEEQKQTYLPKLAKGEFIGSFGLTEPNHGSDPSSMETRLIKH
                     GDEYILKGAKMWITNSPVCDIAVVWAKNEDGHVVGCIVERDMKGFTTPEIKGKWSLRA
                     SITGELIFDDVRIPSKNILPLAIGLKAPLSCLSSARFGISCGVLGAAMDCLHHAVEYS
                     KERNQFGKPLAAFQLTQKKLAEMLTELTKAQLLSLQLGKLNIEGKITPAQISMGKRNN
                     VETALYIARECRQICGGMGITQDFPFMRHAMNLESVITYEGTHEVHLLITGMDITGVN
                     AFN"
     gene            15703..16062
                     /gene="folB"
                     /locus_tag="PLL59_07230"
     CDS             15703..16062
                     /gene="folB"
                     /locus_tag="PLL59_07230"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HMY23637.1"
                     /translation="MQKKVWIGLEGMEFYAYHGVYEEERIKGANFIVDVMIYTDAENA
                     MLHDELSGTVNYELVYQAVAKNMQTPVQLIEHLAYKIITDIRTFVVKEDLIRIKIKKM
                     QPPLNGNVLASVVEVED"
     gene            16135..16205
                     /locus_tag="PLL59_07235"
     tRNA            16135..16205
                     /locus_tag="PLL59_07235"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16167..16169,aa:Cys,seq:gca)
BASE COUNT         5289 a         2639 c         2814 g         5464 t
ORIGIN      
        1 cgaagagcaa aagaaaaagg tgtaaatatt ttattgccaa aagactctgt aattgctgat
       61 aagtttgata atgcggcaaa cacgcaaatt actataaaca ctgcaatacc tgaaggatgg
      121 atgggtttag atattgcaca tgaggctatt caagaatttg aaactaccat tatgacatca
      181 aaaaccatac tttggaacgg accgatgggc gtatttgaaa tggataattt tcaaaatgga
      241 acaaaaagta tagccgttgc ggtggcacat gctacagcag aaggtgcctt tagtttagtt
      301 ggcggtggcg atagtgttgc ggctgttaat aaatttgaat tggcagagaa ggttagctat
      361 gtcagcacag gcggtggtgc cttattagaa ttttttgaag gaaaaacatt gccaggcgta
      421 gctgctttgt tataattttt ttaaaaaaaa tagtgcgagg tctcgtcgaa gacgagacct
      481 cgctgtcatt aaagaagctt attttttgtc ttctttactt gcttcctttt tctctacaac
      541 agcaacgcta ctgtctttta tttgtgtcgt gctgtcttgt aaagtcgata cagtagagtc
      601 cggtgtttcg atagtaacat ctgtaatctc tggtgttgct ggtttcgact tgaaccagtc
      661 gcatgatgta gctaataagc ctactgccat gcaaacaaaa aatacttttt tcattttaaa
      721 ttggtttggt tactactata tacgctatcg cgttttgttt agttcgttta agtataaata
      781 ttaagatatt ttaagaatac accttttgct aagtagattt gattaacatt tattcagcaa
      841 aataatcgat gccaaaatgt tttaaaatga gcgttatgcc tactaaaaaa atgaggaata
      901 atacgagtac tctgaatttt tctggaggaa ttttatctaa aattattttg ccaatataac
      961 taccaacaat actcactatt actaaaaaag gaataattac aatgatgtcc ttatgaatgt
     1021 agccatttac aaaatatact atgcttcttg aaaaatcaac ggctaaatca atggctgctg
     1081 aggttgccac aaagacagac ttctcaacat taaatgctgt cattgtcacc cctcgaatgg
     1141 caccgccagt gcccaataat cctgctacaa agccagatat actgccacct ataaatccat
     1201 ttttttggga aggctcaaca cgtatttctt ttttgatata gaaaacaata ctgatgatta
     1261 ataagaaaac acctaatgcc aattgtaaat agtcaatgtt gatatattta cttgccaccg
     1321 caccaataat aacaaatatt actgcaggta caccaatgcg taaaataatc tgtttgttta
     1381 aaccattttt aaataaagcg attttggaaa tattactaaa tacatgaaaa acagcggtga
     1441 tgcctaaaac ggtttgaaag tcaaaaaagt aattggataa tggtacaaaa aatgtggaag
     1501 aaccaaagcc accaatagtt cccaagattt ccgcaattac ggctaataac agatatatgg
     1561 ctgtggtttt tatcgacatg ttttaaggat atggatactc tacacaattt ttttctgttt
     1621 cccaaagctt gatataaagc gcatattttt catctaattg ttgtcgaatg atgtcatata
     1681 ttacaatagc aatattttct gccgtcggat ttaattgtgc aaattcttta gtatctaaat
     1741 tcaaattttt atgatcaaat cggtcttcaa tttcttcttt gataattttt tttataatct
     1801 tcaaatcaat gacatagcct gtttctgcat cgatttctcc gactactttt aattctaaat
     1861 tataattgtg tccatggaaa ttctcattgg cacaaagccc gaaaactgct ttgttctttt
     1921 ctgtactcca tttctcattc caaagtttat gtgcagcact aaaatttgcc tttcgaatta
     1981 cagccatttt tttcatgctc taaagttaca atttaggata gtaattacga taataaatta
     2041 tgcttatgga aattaaattg aaataaactg tgtataaaaa tcaacgcact ttttacgcac
     2101 actcatgact tacagctata ttcgtacatg ctttttaaag atgtaatcgg acagacagaa
     2161 attaaacaac atttactcga cattgctcat tctggcacta ttccacatgc gcaattattt
     2221 ttatcacaac ctggtgctgg tggcttggcc ttagccttag ccttcacaca atatattatt
     2281 tgtgaaaaca aacaagcaga agacagttgt ggcacttgcc ctgcctgcgt gaaagcacaa
     2341 aaactcgtgc atcctgatat acattttacg tatccaatta ttgcgaaaaa accatcacca
     2401 catccaagct taagtacgga ctatattcaa gaatggcgaa aggaaattat caaaaatgct
     2461 tatataaatg agttagaatg gctgcaagca attgatgccg aaaataaaca aggtaatata
     2521 tcttctgacg aagccagaaa cattataaaa ggattaaact taacagcctt tgaagcaaac
     2581 tataaaattc atatcatttg gatggctgaa gcactttcaa aagaaggtaa tattttatta
     2641 aaactgctcg aagaaccacc agctaatacg gtgcttattc taattgcaga aaatcaagaa
     2701 gaaattttgc ctaccattct ttctcgctgc cagatactga aaataaatag gattaatgac
     2761 gatgcgattg caactgcatt attatctaaa ggtgttccgc aatctaacac acaacacatt
     2821 gcttatttag ctgatggtaa ttttaacgaa gcgataaaac taatcgatag tgaaacaggc
     2881 gccaacacac gattgctaac acaatggctc gattattcta ttaatttaaa aactggtgag
     2941 cttttaaaat ggacagagga aaatgcaaaa ctcggtcgag aaaatcttaa atctttttta
     3001 gaatatacga cacatatttt tcgagagtcg ctgatgctga tgcatattcc aaattatatt
     3061 cctcgattgg ccaatgaaga aaataattta gcacttcgta tcaataaatt tatgaaccat
     3121 gaaaagctcg ctaaattgtt ggagctgatt aataaaaaac actacgaaat agaaagaaat
     3181 gttagctcca aaatggtgtt gatggatatg tcgctacaaa tgcgtaaaat tattagagga
     3241 aaataaactt ataataatct ttttgtatat ttgcataata ttaatcataa taatatttcc
     3301 tatgagattg ataccgctct ttttactaca ctttgtcata agcaatataa cgtattcaca
     3361 agataaactt gtccgaaaaa atagtgaaat tgtattgtca aaagtcgtag agattggaca
     3421 aactgatatt aaatataaga aatttgataa tttagacggt ccaatttaca cagaagataa
     3481 atcaaacttt gtctctgtaa tatttgaaaa tggcaaaata gaagcaattg tttcttcacc
     3541 acttactgca aatgatagac atttcggaaa ggcgagattt acacctttac aaccaaaata
     3601 taaaaatagt attgatttcg atttttttag cttttatact gcagatgtct ctttgcaata
     3661 tacacgatac ttgaaaaatg agcattttgc aatcacagtg ccaattcgag tcggttggta
     3721 ttataatggc tttaatggaa tgtatttact aatggaagaa gataggttgt attttcagga
     3781 aaacaatagt cagtcgtatt attactctta tccagcagat aaaccgaaag gatttaaatt
     3841 gttgagtggt attaatgtaa aatatttttt taaagaagca gttaagttta gaggttatat
     3901 tgcgccagaa tttgcatttg gcgtacataa agaaactaac aaatattatt tctatgattc
     3961 caattcgagc aatacagaaa gtaaagagct ttcacacacc gctggtttgt taggtatatt
     4021 gggtgttttt ggctttcaag caactccatc ttccaagata tttataaaac ttgaaatggc
     4081 tggtggttat ggtggtttaa ttagcaaaaa tatatacgac catcatacat tcgttggcag
     4141 ggcaacattt gccctaggcg tcaattttaa gaaaaaacaa gattaattcg taacattttt
     4201 tcgaatattt ttatagtaga tttagtttag taaatgcctg attaacattt gcgtacaact
     4261 tcatgacaaa aatctcattt tttaatgtaa ttttgcttta caacaagatg ttgtacaaat
     4321 ttattcttaa caattcagct gactttttat aatgtattta tgataagtca actacacaat
     4381 aatatataaa atgagttgta atagttgcgg aacttcgggc tctacaccag gaggttgcag
     4441 aaataatggc cattgtgcca caggtggttg ttcaaaatta gaagttcatg attggctgag
     4501 cgatattaat gctgatccgt atgcaaaata taatgtacat gaagtgagtt tcaaaaatgg
     4561 ctctcgtaaa ggtttttata gaaatgataa acgctttgat gtgtttactg gtgattacgt
     4621 tattgtagag gctgcacaag gttatgactt aggacaggtg acactgagtg gcgaattggt
     4681 gcgtttgcaa atgaaacgca agcgcgtgaa agatgcagaa gaaaatctgc cgactatcat
     4741 gcgaatagcc aaagacaatg aactcgattt gatggagcag gcgagagcca aagaaaaaca
     4801 aacgatgtta aaagctcgtg tattggcacg caacttaggt ttggatatga aacttggtga
     4861 tgtgcaatat caagctgatg gcaaaaaagc tacattttac tatactgccg acggcagagt
     4921 ggactttcgc gaattgataa aagtactcgc tactgagttt aaagttaaaa tagaaatgcg
     4981 ccaaattggt gctcgccaag aggcaggaat tgttggcggc atcggctcgt gcggtcgtga
     5041 attgtgttgc tcaacgtggt tgacgagttt taaatcagtc aatacaacgg ctgcgcgtta
     5101 tcaaaattta tctatcaacc aaacaaaatt aagtggacaa tgtggccgac ttaaatgttg
     5161 tttaaattat gagttagata cctatttaga tgcattgcgc tttgtacctg aaaaagcaga
     5221 atatttacaa ttgcaaagtg gtgatgccaa attaatgaaa acagatattc taaaaggttt
     5281 gatgtattat acagtcaaag gttctggcgt tttctatcca attactttgg aaaatgtgaa
     5341 gttggtatta gaaatgaatg cgaatgggaa aaaacctgct gagataacag atttagcaga
     5401 agtagaagaa aaatctgaag aagaaagcat tgaatttgct gatgtggttg gtcaaatttc
     5461 tttggcaagc ttagatagaa aaaataaaaa gcatcaccca aaaaacaaac ctaatactct
     5521 aaagcaacaa cgcgacaata ctacacaaag gcaaatgcct acacaagagc aacaagcaaa
     5581 cagacatcaa agtaataaac aacacggacg ttccaaccga aatcagcaaa atcgtccgaa
     5641 taaatcagcg aatcaaagcg ataataaaaa tcaaaataaa gacaataaac aagattaaac
     5701 gtatttttct gtaaaaaata ataatgtttt ttctacgagt attttcgatg ctattggtaa
     5761 gttttcagaa tgccaaggat gtttgccacc aaaagtatgg tctgcatctt caatgagtag
     5821 tatctcgctt tttggattaa tttgatgtag tgcattcgcc attactggag aaacagaatt
     5881 gtcatttgtg ccatgaataa tcaaccatgg aattgatagt gaagttgcgg cttttgttat
     5941 atctaattcg tttttatttt ttagataatc ttcatagagt tgataatata atggcatatt
     6001 ttgttgtgta cgcgagttga aagtataagc aacattttca attttccatt tttcaatatc
     6061 catattttgg aaaaaatagg agatgtcttg tatgcttgcc caagtgcaca atttttttat
     6121 tcgtaattca tttgctgcct ttaaaattga aattccacca ccacggctgt gtccaatcaa
     6181 atatatttca ttgttgtttc cttcaaagtc gtttgcattg ttatagatgt aatcaataac
     6241 aaagctcaaa tcatttattt cttttgaaaa attattttga ccaaaagctt ctaagttaac
     6301 aaaatcacat agctgctgtg cggtagtgcc attgtgagaa aaattatatt ttacaaatag
     6361 aaaaccattt tgcgcaaagt aatctgcgat aatatcaaaa tgtccccaat ttttaaaacc
     6421 tttaaatcca tgagagaaaa taataatagg ttttctttgt ttattttcta tataacgaat
     6481 gtcaatgctt attggttttt cgtttatacc ttttataata aattctattt tctgcatttt
     6541 taatttttgt atattgtaaa tagcatttat aactttacct ttgacaaaaa taattaatcg
     6601 ttgagtaata cggtcgataa tttaaaagca cctattgaac aggaacttga aatttttgaa
     6661 caaaagtttc gcgaggtcat gcgcagccac gtatcgttat tagataaagt gaactattat
     6721 atctatcaac gcaaaggcaa acaagtgcgt ccgatgttta tttttttagc ggcaaagatg
     6781 tgtggcgaag taacagaatc aacttatgtt ggtgcctcat tagtggagtt gctgcataca
     6841 gcaacattgg tgcatgatga tgttgttgat gatgcgtatg aacgcagagg ttttttttct
     6901 ataaatgcat tatggaaaaa taagattgct gtgttggttg gtgattattt tttgtctaag
     6961 ggtttattgt tagcactaga tacgaaaaat tataatttac tacatattac ttctacggca
     7021 gttaagcgca tgagtgaagg tgaattattg caaatggaga aagcgagaaa attagatatc
     7081 gacgaaagcg tttatcttga aattatcact aacaaaacgg catcgctgat tgctgcagcc
     7141 tgcgaagtgg gcgccgcatc aacaactact gatactgcat tgatagaaaa aatgagactg
     7201 attggtgagt atattggaat tgcttttcaa attaaagatg atttatttga ttatggcgaa
     7261 gcagatatcg gcaaacctcg aggcattgat attaaagaac ggaaaatgac attgcctttg
     7321 atttatacat taaataatgc ttctaaagaa gaaaaaaaat ggataatcaa tacggtgaaa
     7381 aaccataatg aaaataagca acgtgtgcag gaattgattc agtttattca taaaaaagga
     7441 ggcattcaat acacagaaga aaagatgcat gaatataaat tgcgcgcttt tgaaattatt
     7501 aaaacgatac ctgagtcgcc agcaaaaaca ggattgattg atttggtaaa ttatattaca
     7561 gaacgtaatt attaagatgt ctatagcaca acaagcacca aataataaag agaaattctt
     7621 tgtacaatca ctcacattag tggtaggttt ggctttgctc ctcgttaaat tttttgctta
     7681 ttttactact aattccaatg ccattttaac agatgcctta gaaagtattg tgaatattgt
     7741 tgcaggcagt tttgggttgt atagcttgta tctcgctgca aaacctaatg atgaagacca
     7801 tccatacgga cacggcaaag tggagtttat ttcagcaagc atagaaggtt ctttaattgc
     7861 agcaacgggc attgcaatga ttataaaatc gagctataat tttttcgttc cgacttcatt
     7921 ggataactta gatgttggtt tgatgctcat tgtaattgct ggtattatta attttatttt
     7981 aggcaaaatt gcggttgctt atggcaaaaa aaatagttca ctcacactta ttgccagtgg
     8041 tgaacattta aaagttgatg cctatacttc ggtaggtatt ctattaggtt taggtattgt
     8101 ttattttact gattttttat ggttggataa tgtcgttgct attttaatgg gaatttttat
     8161 tgtgtataat ggtattaaaa ttcttaagaa atctgttgct ggtattatgg atgaagcaga
     8221 ttttttctta ttggaaaagg tagtggtgca actgaatgat aatagacgta tgaattggat
     8281 tgacattcat aatttacgca ttattaaatt tggtgaaaaa attcatatag attgccatac
     8341 tacaatacca tggtatttta atgcgaatga agtacacgaa gaattaaaat tagtcgagtc
     8401 aaacatcaaa cagattattc ccaataattt agaaactttt atacatgcag atccttgctt
     8461 gcctaaatcg tgtcgaagct gtttaaaaac agattgtaag gtcagacaat ttccatttga
     8521 acgtaaaata gagtggacac tcgaaaatgt gatgaaaaat gaaaaacatt ttttggaaaa
     8581 aacttgacaa acccgacaaa atgtattaat tttgtgacaa atgacgtgtt gtgaagaaaa
     8641 agactgtaaa atatcaaatc cgtgaagacg aaataataca taaagtagca gaaaaagaaa
     8701 tagcctatta tagagcatca tctgttcctg ctttattaaa tgatgataca catttcttga
     8761 ttgataaaac aaggaaaggt atatcgatgc atcagattca acaacagatg aataaatatg
     8821 atttgacgct taaagaaatg gcaaatgttt taaatgtttc agagcgtacc ttacaacgat
     8881 atgaaacaca tgatttactt ggaaaagatg cttcagaacg ggctttacat ttacaacgct
     8941 tatacgaaag aggtacagaa gtttttggtt ccttgtcagt tttttgtgag tggatgaaaa
     9001 cgcctgtttt attatataat catgagttgc ctatcagtta tcttgatacg atatttggtt
     9061 ttgaattgtt agaacaagaa ttaggcagaa ttgaacatgg catcttcgca taattccaat
     9121 gatagtttat aggatttgta ataaattata tgctaatgac ctgcaaggta ttggcgcaaa
     9181 aatgtatgga ggcagatgga ataatgttgg taatgcggtg gtttatactt catcatcacg
     9241 tgcattggct gcgcttgaag tattagtaca tttgccttca actacgctta aaaagcttga
     9301 ttttacagtg gtttcgatag aaattcctga aaatagtgta gaagaaattt tttatgatga
     9361 tattaaacgt gattttcaaa ttaataggtt tagtgcattt ttcaaaaata tcggagataa
     9421 ttggttgcga gaaaataaga gtttgttgtt aaaagttcct tcagttgtta ttcatgaaga
     9481 gatgaattat ttaattaatc cgaatcataa agactttaat aaagtaaaat tgacagagac
     9541 gaaattattt agattcgacg atcggcttgc gaagtgattt ttatttttta cgcaattcaa
     9601 aattttgtcc taaatatact tttcttacct gttcatcaga tgctaaatca tctgcagtgc
     9661 cttgttttat aatttttcct tcgaacagta aataggctct atctgtaatg gataatgttt
     9721 catgcacatt atgatcagta attaaaattc cgatattttt aaatttcagt ttttctacaa
     9781 tgtgttgtat gtcttcgact gctatagggt cgatgccagc aaatggttcg tctaataaaa
     9841 taaattttgg atctgatgct aatgcacgag caatctctgt tctgcgacgc tcgccacctg
     9901 aaagtaattt tccttttgtt ttgcgcacat gttttaatcc aaattcttct aataatgatt
     9961 ctagtttctc tttttgtgct gttttactta aatcactaaa ttctagaatt gctttgatat
    10021 tatcttccac acttagtgtt ctgaaaacgg aattttcttg aggcagatat ccgacaccaa
    10081 gttttgcgcg tttgtacatg gctacactcg aaatatcttt attatctaaa aatactttac
    10141 cttgattggg tgtaactaat ccaaccgtaa tgtaaaatgt agttgttttt ccagcgccat
    10201 ttggaccaag taaaccgaca cattctcctt gatttacttc aatggaaaca ttatcgacta
    10261 cttttcgatt gctatatgtt tttactagat tttctgcgcg tagtaccatg ctttcaaatc
    10321 ttttaatcga aattcaattt ttgtttgtcc attccagata tttgcataca tctgaaaaca
    10381 aatatcaaca gtttcgttat tttccaaaat tttcatataa tcgcccatgc caaatcctac
    10441 gccgtttcgt gggttttgtc catctttcaa aaggtttaat ttgagatgat tgccgtttaa
    10501 tactttacta taaccagtat catagacatc tttggtaacg aagttaggac gcatattgcc
    10561 aggtccaaat ggtcccattt gctctacgat gttgaagaac ttttcattga tatcattaag
    10621 ctcgataatg gcatcaattt caatttgagg aatgagttgt tcgtctaaaa ttctttcgct
    10681 aacagttttt tcgaatgctt caataaaagt atcgatttta tcaatttcta tagttaaacc
    10741 agcagcatat ttatggccac caaactgtac taaatgctca ctacaagcat caatagcgtc
    10801 gtgtaagtcg aaacctggca cggagcgacc cgagcctact gcttttccat ttgacgatgc
    10861 taaaataatg gtgggccgat gatatttctc tattaagcga gatgcaacaa taccgattac
    10921 acctttatgc caatgctctt gatataatac ggtagatttt ttttgaatta ataaatcatt
    10981 ggtgtcaatc attgcaaatg cttcttccgt aatgtcttta tctaaagtct ttctatcgct
    11041 attattttgc tgcacttttt ttgttttttc aactgcaaca tcataatctt tttctattaa
    11101 taaagttaca gcttctgagc catgtgctat tcttcctgca gcatttattc ttggtccgat
    11161 aataaaaact aaatcattga tatctaatat tcgtgtgagt ttgagctcgc tcatcaatgc
    11221 tttaaaacca gctctggtat ttgtgtttac tttatctaca ccaaatttag cgagtattct
    11281 gttctcgtcc gtaataggta ctaaatcaga ggcaatgctg acggctacta aatccagatg
    11341 ttcatacact ctttcaatgt ctaattggtt tttaagagta tatgcctgaa taagtttgaa
    11401 acctattcca cagccactca gttccttaaa aggataagta caatcttccc gttttggatc
    11461 taaaatagcg actgcacttg gaattgtatc acctggtaaa tgatggtcgc aaatgataaa
    11521 atcgatgcct ttttcattag cataatttat tttatcatgt gcttttatac cacagtctaa
    11581 tgcaataatc aattgtacat tattttctgc tgcgtaatca attccttgta gggaaattcc
    11641 atatccttct gtgtatctat caggaatata atattccaaa tttggataaa tatctttgaa
    11701 gaaagaatag actaatgata cagcagtggt gccatcaaca tcataatcgc cgtagattag
    11761 aattttttca tttttcaaaa tagcgagttc aatacgttca atggctttat ccatatcttt
    11821 catcaggaac ggatcgtgta atttttctaa acttggtcta aaaaaatctt tagcttcatc
    11881 aaatgttttg atatttcgca ataccaataa tttgcataac gttgggtgaa tgtttaattg
    11941 cttttgcaat tcttgcactg cattttcgtc agcttctaag tatgtccatc ttttattttg
    12001 cagcataatt ttaaagaatg tcaaatttat caaattgtta ccgtaataaa taattttaat
    12061 ttgacattat actatttttt agaaaatagt ttaaatcttc tagtgtcatc acttagattt
    12121 ttgatattgt attacttttc tataaatttt agaatatgtg aataaattga tgttttatag
    12181 tctaactatt attttaattt tgtattgcta atgtgtatga aaaagataat ttataccacc
    12241 ttaggtgtgg cttctgttgc aaacgaaaag tttaaagaat tactggaaga tttaattcaa
    12301 aataatcagt ttacggaaga tgaaggcaaa cgtattgttg atggcttttt tttcgatttg
    12361 cgccaacaat tggatttggt gcaaggcaat gtgcagtcta aactggacga tattatgaag
    12421 aaattcggtg tttctgattt ttatagtttg aaacaggaat ttgaaaatta tgtgcaagat
    12481 gtaaaatcaa atccaacgct tttattaaaa ttaccgtcta agaaataaaa agtcgcctta
    12541 aaaggcgact acttcggatt cttatgtttc tattttatgt taatgcaaag ttaaggctaa
    12601 tttgaagaac tagttttgac ttgtatcaaa taaatcacat atagtatctt tttgtaatgt
    12661 ggcgttttta actagaaaag atcaaaatca tcagtttttt taccttcctc atctttcatg
    12721 aaattaccct ttgatggtac agttttatag ctgttgctat attcttgcat ttcatcataa
    12781 agtccaccag catcatagaa tcgaccaaag tgtttgttga atcttaattc aacactgcct
    12841 gttggtccgt ttctatgctt ggcaataatg atttctgaca ttccttttaa atgtgcgcca
    12901 ttagctgctt catctaattt ataatattca ggacgataca agaacataac catatcggca
    12961 tcttgttcta tagagcctga ttcacgtaag tcagatagct gtggtttctt atcgccaccg
    13021 cgcgtctcca cgcttcggtt taattgtgac aatgcgatta caggaatgtt taattctttt
    13081 gccaagcctt ttaaggagcg tgaaatattg gagatttctt gctctctatt catgcctttg
    13141 tcgccgccgc cactcattaa ttgtaagtaa tcgatgatga ccatttttat accgtgcgca
    13201 ttttgcaatc tacggcattg tgcacgaagc tcatatacat tgattgctgg ttggtcattg
    13261 atgtatattg gtgaagagga aagacgttct actttgtctc ttaaaatggc gtattcgttt
    13321 tcactcattc ttccgtttgc tactttttga gcatcaatct cgcactctaa tgaaataaga
    13381 cgttttgcta attgcacggc gcccatctct aatgagaaaa ttgcaactgg catattgtga
    13441 accacagcag catttcttgc tatatttagt acgaatgcgg ttttacccat agatggacgt
    13501 gcagcaacta tgactaaatc agttgctttc cagcctgatg tcattctgtc taattcaaca
    13561 taaccagatg gcactgagtt ttctaataaa ccatcttctc tgctttgcaa actgtctata
    13621 tcagttattg atttagatac taattgtcca atattttgta ccgaacgact gatgttttta
    13681 tcagcaattt cataaatctt tttctctgca ccatctaata aaacaaaaac atcagttgtg
    13741 tcctcaaaag catcgcgaat aatatcacta gatatgcgaa ttaattcacg ctgtataaat
    13801 ttttgtacaa taatacgtgc atgaaattct acattggctg atgatgcaat tttattggtg
    13861 agctcactga tataaaaagc accgccaact tcttctaatt tgccactttt tcgtaagtct
    13921 tcggtaaccg ttaataagtc gatcggatgt gacttgccaa ataatgattg aattgaacgg
    13981 aagatagtag catgcgcatc tacataaaaa ctttctggtt ttaatacgtc atttacttct
    14041 gcaatagctc ctttttcgat gagcaatgca cctaatacgg cttcttctaa ttcacgagcc
    14101 tgaggcggaa ctttgccata taacatatta gacatatctt ctgtcttatt tctagtaaat
    14161 cgtgtagttt ttttagtaaa attgcttgtt tgttcttcca tgggacaacg aaaatacaat
    14221 ataaaatttg ttgtaaatag cgtaaaaatc aactacaatg ttaataacga atgtggatac
    14281 attgttgata gtacgaaccc gcgtgaaaca tgaggtttat tacttgaaat tgaacgtttt
    14341 aattggaatt tcaacacaaa caaattaata attacataat ttattgctgt tagaaacttt
    14401 ttttcacccc aattttaaac aagtttatgc actattttat ataaatttca aggtgttatt
    14461 tatgtatata attactttat attgaattaa ttaaatgcat ttacgccagt aatatccata
    14521 cctgtaataa gtaaatgcac ttcgtgcgtt ccttcatagg tgattacact ttctaagttc
    14581 atggcatgac gcataaaagg aaaatcctgt gtgatgccca ttccaccaca tatttgtcgg
    14641 cattcacgag ctatatataa tgcagtttca acattattgc gcttgcccat cgatatttgt
    14701 gcaggtgtta tttttccttc tatatttaat tttcctaact gtaaagaaag taattgcgct
    14761 ttagtaagtt cagttagcat ttctgctaat tttttctgtg ttaattgaaa tgctgctaat
    14821 ggttttccga attgattacg ttctttactg tattctacag catgatgcaa gcaatccatt
    14881 gcagctccaa gcacaccaca tgaaatgcca aatctggcgg aagacagaca tgataatggc
    14941 gcttttaatc ctattgctaa tggtaaaata ttttttgatg gaatacgcac atcatcaaat
    15001 attagctctc cagtaataga ggcacgtaaa ctccattttc ctttgatttc tggtgtagta
    15061 aagcctttca tatcgcgttc tactatacag cctacgacat gaccatcttc attttttgcc
    15121 caaacaacag cgatatcaca tacgggtgaa ttggtaatcc acatttttgc accttttaaa
    15181 atatattcat caccatgttt aatcaatctt gtttccattg atgaagggtc gctgccgtga
    15241 ttaggttctg ttaatccaaa gcttccaata aattctcctt ttgctaattt tggtaaataa
    15301 gtttgttttt gttcttcgct accaaatttc caaattggat acatcactaa tgagctttgt
    15361 acgctggcgc aactacgaat accagaatcg ccacgttcga gctcttgcat aatcaatccg
    15421 taagcaattg catctaagcc accgccacca tattgcgttg gtatatgagg gccaaatgca
    15481 cctatttcac cgagttcttt tattaaatgt ttcggaaatg tatttttctc tgcacaatct
    15541 tcgattattg gaattacagc cgtatttacc caagctctga cgctatcgcg aattagcttt
    15601 tgttcgtctg ttaattgtgc atctaagttt aaataatcaa aaccacggaa agaagaagca
    15661 tatctattag ccataatata ctatttttgt taaaacaaag gtatgcaaaa aaaagtatgg
    15721 ataggattgg aaggaatgga attttatgcc tatcatggcg tatatgaaga agaaagaata
    15781 aaaggtgcaa attttattgt tgatgtaatg atatatactg atgcggaaaa tgccatgttg
    15841 cacgacgaat tatcaggaac agtaaattat gaattagtgt atcaagcagt tgcaaaaaat
    15901 atgcaaacac ctgtgcagct cattgaacat ttggcatata aaataattac tgatattcgc
    15961 acatttgtag tgaaagaaga ccttattaga ataaaaatta aaaaaatgca accaccattg
    16021 aacggaaatg tattggctag cgttgtagaa gtggaagatt gattatttta taaaaagtga
    16081 attctatttt tgttttctgt aaaaataatg tacatttgca ttccaaaaaa taatggtcgg
    16141 ttggccgaga ggctaggcag agctctgcaa aagctcgtac ggcggttcga atccgccatc
    16201 gacctc
//
LOCUS       DAOQQV010000057        15908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1159_length_15908_cov_7.653756, whole genome shotgun sequence.
ACCESSION   DAOQQV010000057 DAOQQV010000000
VERSION     DAOQQV010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15908
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1159_length_15908_cov_7.653756"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..825)
                     /locus_tag="PLL59_07240"
     CDS             complement(4..825)
                     /locus_tag="PLL59_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23638.1"
                     /translation="MKLHFLLVLAFACLLCNSLIAGDNIFYTIKTNSADTNSNITVQT
                     NENTIASQHDKAFYKLVSESDTALYLKKMTLQNGKLVYSKDITIVSKLDNVSSKSLKN
                     AFSSLKLKDKISFSLGTIMMPIKMRFSSEDSNHISKKDFSFSGSFNVGATAGMRVSLN
                     KENSLSASILFGLSVSHVEIDERESQGHYTDKTPVAAMSPSMNFIVSYKKFNAGFAVG
                     WDILNQTIGKYWIYNKKTWLGIGIGVQITDTENSQAESRIKKQMQAEQEGNASSK"
     gene            complement(1141..1983)
                     /locus_tag="PLL59_07245"
     CDS             complement(1141..1983)
                     /locus_tag="PLL59_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMY23639.1"
                     /translation="MAVRKFSYEFDGVSYYRFSVYRLGSNVQTVYTFVLDDVLIDTAQ
                     SHNRENILHVAQKHDIKKIILTHHHEDHSGNVGFLIDELKIDAYAHPETVRLLKRGLK
                     LSPLAKLLSGSVGHAHLLPLTENSIIQTANYNLQAIYTPGHCKDHYCYYEANKGWLFS
                     GDLYVADKIKYFAKFESLLTQIESLKKLIALDFDVLFCSHNPKTKDGKQRLKNKLQFF
                     EDFAGTVTTLYNQGHNARQIFALMGMKENYVNKYLTFGAFCSENMVHSVVADLKKQKL
                     ALKK"
     gene            2078..2950
                     /locus_tag="PLL59_07250"
     CDS             2078..2950
                     /locus_tag="PLL59_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23640.1"
                     /translation="MKKYVIGGTLLLLIVAGVTFFLTKTMMQMQIDKAVQIHIKDKVP
                     LDAQIDSSILISIINDLQTKINVNDELKIKLDETFDVPLRMNLKVPLNTEVFMDQELD
                     LKFDLPVDINLDQTEMPLKNLVIPFNKKLQINDSLAVDFSIPLDTKIRTNFKHFFNIS
                     LPVKATIPVKVNIPVNQPLQVQDTLVLSMQDYQIPLKTVIPVVAKVPIKQLVKIQGEL
                     EVPVDQKIKIPLSKVISTPVLQPFTASVKTTNDLQTSFKSSLKANASFSEPLRVIQMD
                     SLRIEPSKIKFSVK"
     gene            complement(2964..3305)
                     /locus_tag="PLL59_07255"
     CDS             complement(2964..3305)
                     /locus_tag="PLL59_07255"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HMY23641.1"
                     /translation="MENPFLQIKSITPAEMMYLKNKTALLNEEQLKSFTILYNSKRRE
                     SDLLLIIACAGFFGVAGIQRFLVGQILMGLLFFFTAGFCGIGTIIDIINNKELANEYN
                     AKMADECLQLI"
     gene            complement(3280..3594)
                     /locus_tag="PLL59_07260"
     CDS             complement(3280..3594)
                     /locus_tag="PLL59_07260"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HMY23642.1"
                     /translation="MHRIKKYFSLELCFWIITLVYLYFIDTTAPHLSFCIFKLFDVTA
                     CNGCGIGKSIALLIHGEYKQSISTHWLGTFALAMIVYRIVQLFFTIYKTNRQNGKSIL
                     TN"
     gene            complement(3605..4423)
                     /locus_tag="PLL59_07265"
     CDS             complement(3605..4423)
                     /locus_tag="PLL59_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMY23643.1"
                     /translation="MSFKNKTVFISGATRGIGHAIGMRLAKEGANIVIAAKSTTENPK
                     LGGTIYTAAEDMTNAGGKGLAIKCDIRNDDEVEAAVKQTVDVFGGIDILINNASAINL
                     SPLEQLEMKRYDLMQDINTRGTFLCSKMCMPYLLKSENPHILMLSPPLTSINERWFAG
                     HTGYSIAKFGMSLVVLGLGGEYKGKVGVNALWPRTTIATAAVLNLLGGESVANTSRKP
                     SIMADAAYCILKRDGKKTTGNFFIDDEVLLSEGISDLEQYAVKPGAELTPDFFV"
     gene            4616..5890
                     /locus_tag="PLL59_07270"
     CDS             4616..5890
                     /locus_tag="PLL59_07270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HMY23644.1"
                     /translation="MRKVVFIDGVRTPFLKSGTDFMDLMSYQLGQFAIKGLIDKTGID
                     VNEIDKVVYGNVISNVRTENVAREAAVTAGIPVKAPCNTVKQACISANQAIANAVDSI
                     QLGRADVIVSGGTECTSDTPIGYTKEMRKKLFSAQKLSTPMEYAKFATTLRPTDFLPD
                     RPSVSEFITGRTMGQDCEILAQKFGVTREEQDQLAAASHQNAAKAFEAGLMNDIVPVQ
                     LPPNFSPITKDNGVRGDSTVEKMAKLKPAFDKYVGTITAANASFLTDGATAVLLSSEE
                     KAKELGLTPKAEIVDYAFTGGDLYEELLLGPAYSIAAVLKQTGLTIDDIDVFEIHEAF
                     AGQVLANLNALKSAEWCKKQLGLDKPIGQIPLNKVNAWGGSLSIGHPFGATGGRLLTM
                     AANRLQKDGGKYALLAACAAGAHGHAMIIKKY"
     gene            5955..8141
                     /locus_tag="PLL59_07275"
     CDS             5955..8141
                     /locus_tag="PLL59_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMY23645.1"
                     /translation="MVVLKREDYISIEKNSNGVCTIWMDQKDEKINKIGPDLIGLFES
                     VFEELNADDSIKAFVLSSKKKDFIAGADIDAFAKVEKPGDWKPIARKGHEILNQIEKS
                     KKPIVAAIHGNALGAGLEIAMACHARVLSNDKSTKVALPEVKLGLLPGGGGTQRLPRL
                     VGLQAALDMMLTGKNVFAYPALKMGLADKVVHVSALQKVAQKFALELVDNPIKRERNE
                     LDIKAGFPSIQKSLTNIVLEAPLVNKIVFDQAKKMVDKQAHGNYPAPYKIMECVEIGW
                     NQGIEKGYEAEVDRFEELILSPVSRQLINIFFAMTDKKKNPYGEDKVKQVDRLGMIGA
                     GFMGAGIAEVSMNDGMHVLLKDINQDMISSAYKTIYDGYNKRVSKKAMSKIELEEKMA
                     LLSGSLNYEDLDNQEIIIEAVFEDLKLKQAILKDVEANAKPNTIFATNTSALPIKHIA
                     ANAKHPELVIGMHYFSPVPKMPLLEIVKTDKTADWVIATCYDVGVRQGKTVIVVHDGP
                     GFYTTRILAPLMNEAQLLLDEGGDIQQIDREMNLFGFPVGPITLADEVGIDVGAHIMS
                     GELMQELLSTRPKFKVSKTLLEISKAGYKGRKNKKGFYKYDEKGKKVSGQVDADIYSF
                     YGGNTRKKFDANDIHMRCAMAMVNEAALCLQENIIESPLDGDIGAVFGLGFPPFRGGP
                     FRYIDSVGAQKVVDILNDLTARFGERFEPAQILLEYAKSGKKFYDK"
     gene            8351..8785
                     /locus_tag="PLL59_07280"
     CDS             8351..8785
                     /locus_tag="PLL59_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMY23646.1"
                     /translation="MFSKACEYGIRASIYIAEQSLQCKKVSLKEVAFAIESPEAYTSK
                     ILQQLVRERIIKSIKGPNGGFMIDNEKIETLKLSAIVLAIDGDSIYEGCGLGLSRCND
                     KMPCPVHHQFKAIRDNLKEMLTSTTLKSLALEVECGLTFLKR"
     gene            8791..9189
                     /locus_tag="PLL59_07285"
     CDS             8791..9189
                     /locus_tag="PLL59_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HMY23647.1"
                     /translation="MQDLRKDITHLDDVKLLVDSFYGKVRQDEKLKDIFDNVIKDRWP
                     EHLEKMYRFWQTVLLDEHTYFGSPFVPHANLPVIAEHFNQWIHLFFETVDENFKGKNA
                     DKAKWQGQRMAEMFHTKIEYYKNNTSIPLL"
     gene            9231..9953
                     /gene="ric"
                     /locus_tag="PLL59_07290"
     CDS             9231..9953
                     /gene="ric"
                     /locus_tag="PLL59_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HMY23648.1"
                     /translation="MSITKETIIGELVANDYRAASVFKKYKLDFCCQGNRTIGEACEL
                     KSVDANALVSDLIATTQNVEAGAIDFQTWPLDLLADYIQKKHHRYVEEKSLEIKPYLD
                     KICRVHGQHHPELFEINEHFTATAGELAMHMKKEELILFPFIKKMEKAKQEGTGLEMP
                     PFGSVQNPIDMMMHEHTTEGERFRKIEELTNNYNPPAEACNTYRVTFALLKEFEADLH
                     LHIHLENNILFPKAIEMEKTFR"
     gene            9950..10333
                     /locus_tag="PLL59_07295"
     CDS             9950..10333
                     /locus_tag="PLL59_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HMY23649.1"
                     /translation="MKKIKIKRIYDEPSSNDGYRILVDRLWPRGISKEKAAIDEWNKD
                     LAPSTELRKWFHHAAELFDLFEEKYRNELSEQTTAIKHVMEINKHRDISLLYGAKDPN
                     INHAIVLLDVIKNKINDYEQSNKKK"
     gene            10308..10766
                     /locus_tag="PLL59_07300"
     CDS             10308..10766
                     /locus_tag="PLL59_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23650.1"
                     /translation="MSSPIKRNELLKPYSRDHHHGLLLCWKIKVGLQKNIELNRISNY
                     VSSFWNSHLKAHFLEEEKYVFTVLPSNDEHIIQAIKEHNSLRAHFEKENMDIATLQSI
                     EMELNAHIRFEERLLFNKIQDAATETQLQQLNEHYNNEYKEEWNDEFWLN"
     gene            10941..11174
                     /locus_tag="PLL59_07305"
     CDS             10941..11174
                     /locus_tag="PLL59_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMY23651.1"
                     /translation="MSKSIFYHAGCPVCVSAEHDIVNLIGAENVEVVHIGQDRSRIEE
                     AETKGVKSVPALVTPNGNVLHINFGASMADVKG"
     gene            11234..11500
                     /locus_tag="PLL59_07310"
     CDS             11234..11500
                     /locus_tag="PLL59_07310"
                     /inference="COORDINATES: protein motif:HMM:NF021169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HMY23652.1"
                     /translation="MQVAVWDTYVTKKDGSIMHFDIIVPENIKDAAVIYNYGLEYLAT
                     KGQQGQTLTATECQFCHIETIRPQWKDAIIEKGYYIYEMENCHS"
     gene            11497..12108
                     /locus_tag="PLL59_07315"
     CDS             11497..12108
                     /locus_tag="PLL59_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HMY23653.1"
                     /translation="MKKSDFNLEQQNHHIESKIIASLERIAQAFRILLWQESVTYALT
                     PIQIQLLIFIHYHQIEKCKVSYLANEFNLSKATISDTIKLLEQKQFIQKIFDQNDSRS
                     YTIKLTKKGKEIALKTSFFSEEISKPIEQLHSEDKQNMLLGLLDIIRHLNSSGVITLQ
                     RMCYTCTYYKSSHNGKQHYCNLLQQKLSIEDLRIDCPEHQQLT"
     gene            12105..12842
                     /locus_tag="PLL59_07320"
     CDS             12105..12842
                     /locus_tag="PLL59_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMY23654.1"
                     /translation="MNNIVSNIQAIQNRINEACEKANRNPNEVKLLLATKTVSAENIK
                     HAIEAGACIIGENKIQEIKEKYESLKSVVHEKHFIGHLQSNKIKEILRYEVSCLQSLD
                     RLDLAEKLQQRLATENKELDVYIQVNTSGEESKFGVAPNDAITLVKSVATLKNIHIKG
                     LMTIGLFSAEHNKVRQCFALLKSIQQSIIRENIEGVEMKELSMGMSGDLEIAIEEGST
                     MIRVGTAIFGNRIYPDSYYWNESKAVN"
     gene            complement(12835..13716)
                     /gene="prmC"
                     /locus_tag="PLL59_07325"
     CDS             complement(12835..13716)
                     /gene="prmC"
                     /locus_tag="PLL59_07325"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HMY23655.1"
                     /translation="MNVHQYKNIILPMLAESFDEREAANLFKYALEDYFHKRYFDIKD
                     FDLNEEEIEELNYIFEKISNHYPIQYIFNKAHFFGLDFYVDENVLIPRPETEELVHWI
                     LTNHADNSLQILDIGTGSGCIAIALKKNKPDWKFFAIDISEKALEVANINANKHHTEI
                     EFFQHDILPTTNFFVDVLLYMERDDAAPLQLDIIVSNPPYILFFEQDKMSSSTLNFEP
                     PTALFVSNDSPILFYEKIADFALLHLTKNGKLYFELNEFNANEVVTMLQQKGFIDIQL
                     KKDLAGKDRMLRCTIIN"
     gene            13800..14813
                     /gene="ribD"
                     /locus_tag="PLL59_07330"
     CDS             13800..14813
                     /gene="ribD"
                     /locus_tag="PLL59_07330"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HMY23656.1"
                     /translation="MHRCFELAKLGKGYVSPNPMVGAVLVHNDNIIGEGYHQQFGEAH
                     AEVNCLNSVAEKDMELISDATLYLNLEPCSHFGKTPPCTDLIIRHKIKNVVISTLDAS
                     DKVNGKGVQRLREHDVNCITGILEKEGSDLNKHFFYFHKHNLPYITLKYAQSHDGYIG
                     VIGKEIKISNDATQRYVHQLRASHQAIAVGKNTVLSDNPSLTVRFATGKNPIRIVLGS
                     EHAIPKDYHVFNNEASTFFYGRINDEAITIQELLQQLAKNNIISLLVEGGANVLQQFI
                     DNNAWNEAHIITAQNNMQIISNENIDTYIKAPVISGTLQENCTLQTDNIQVLKNPNAL
                     SYS"
     gene            14794..15651
                     /locus_tag="PLL59_07335"
     CDS             14794..15651
                     /locus_tag="PLL59_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23657.1"
                     /translation="MLYLILSIICATALVVLFRLFDKWKINSTHAIVFNYIFCVLTGL
                     IFVDDFSVFPKMIQWNGFPIVVLLGFAFILIFLLVGKTALSLGVVTASIAMKLSFVIP
                     VSVAIILYNDSITALKIIGIICAIIAVYLIAYENESKQEASTRALIYPFLIFIGSGLC
                     DTAFNYIQKKMMPTNWDHQVTIGVFAAAMVLGILLHFRDKKLYQWKNVLAGIILGIPN
                     YFSLYFLLKTLSTLPWQSSVIFPVNNLGIVCLSAFIGFIIYKEAFNTRKSIGFILAIS
                     SIIIIGFIA"
     gene            15653..>15908
                     /locus_tag="PLL59_07340"
     CDS             15653..>15908
                     /locus_tag="PLL59_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HMY23658.1"
                     /translation="MYSYYTIHNAVEQVLYKDKASKFYGFAYPVKNEIEIKEKLEFVK
                     AQHPKATHHCYAYRIGIDKNNYRANDDGEPSGTAGKPILGQ"
BASE COUNT         5760 a         2576 c         2687 g         4885 t
ORIGIN      
        1 cttttatttg ctgcttgcat taccttcttg ctcagcttgc atttgcttct tgattctact
       61 ttctgcttgt gaattttccg tatcggtgat ttgtacgccg atgccaatac ctaaccatgt
      121 tttcttatta taaatccaat actttccaat cgtttgattt aagatgtccc aaccaacggc
      181 aaatcctgca ttaaattttt tgtaagaaac tataaaattc atactcggtg acatagcagc
      241 tactggcgtt ttatccgtat agtgtccttg cgactcacgt tcatctattt cgacgtgcga
      301 aactgacaag ccaaataaaa tactggcaga taatgaattt tctttattca aacttactct
      361 cattcctgct gtagcaccta cattaaaact tcctgaaaat gaaaaatctt ttttagaaat
      421 atggttacta tcttctgatg aaaagcgcat ttttataggc atcataatcg ttccaagcga
      481 aaaagaaatt ttgtctttta attttagaga agaaaatgca ttcttcaaac tcttactact
      541 aacattatcc aactttgaaa caatagttat atccttactg taaactaatt ttccattttg
      601 taacgtcatt tttttcaaat aaagtgctgt atcactttca ctcacgagtt tataaaatgc
      661 tttgtcatgt tggctcgcaa ttgtattttc atttgtttgt acagttatat ttgaatttgt
      721 atctgcacta tttgttttta tggtgtaaaa tatattatca ccagcaatta gcgaattgca
      781 caacaaacat gcaaatgcta atacaagtaa aaaatgtaat ttcatacgtc ttatttttta
      841 ttaaaataca ttaatataca aaaaaagtgt cttgacagca aacgccgtca agacaacacg
      901 caaaaaataa acgatgcgaa ctaaaaatgc atgaactaaa tctacatcgt ttattatcat
      961 tatttctctt ctgtatttct ttcgtaaaag cagaaccaca cgcttaaata caataaaact
     1021 gtcataatta attcgtgtgg ttccatggtc gaaaagttta tgtaaaccta catcgagcaa
     1081 ataaatgaac agtccttcgg tatgtaaacg gtaagaattt tgagtaaacg gtaaaacgtg
     1141 ttacttttta agcgctaact tttgcttttt taggtcagca acaacgctat gtaccatatt
     1201 ttctgaacaa aatgcgccga aagtaagata tttattgaca taattttctt tcatgcccat
     1261 cagcgcaaat atttgtcgtg cattatgacc ttgattatat agcgttgtaa cagtgccggc
     1321 aaagtcttca aaaaattgaa gtttattttt aaggcgttgt ttaccatctt ttgttttagg
     1381 attgtgcgaa caaaataaca catcgaaatc aagtgcaatt aattttttca atgactcaat
     1441 ttgcgtaagc aaactttcaa actttgcaaa gtattttatt ttatctgcaa cgtataaatc
     1501 tcctgaaaac aaccaacctt tattggcttc gtagtagcaa taatgatctt tacaatgacc
     1561 tggtgtgtag atggcttgta aattataatt ggcagtttgg ataatactat tttctgtcaa
     1621 tggcaataaa tgtgcatgac caaccgaacc actcaataat ttagccaatg gcgaaagttt
     1681 caaaccacgt tttaacaatc gaacggtttc gggatgagca taagcatcga ttttcaattc
     1741 atctattaaa aatccaacat tgccactgtg gtcttcatga tgatgtgtta gaataatttt
     1801 ttttatgtcg tgcttttgag cgacgtgtaa tatattttct ctgttatggc tttgtgccgt
     1861 gtctatgagc acatcatcca atacaaaggt ataaaccgtt tgtacattac tacctaaacg
     1921 atacacacta aatctataat aagaaacgcc atcaaactca taactaaact tgcgaactgc
     1981 cataaacgaa aataatattt ccaaaaataa aaatacttta ccttggtaaa cattaatttg
     2041 caagaatatt tctattttgt taaataaaat ctttcaaatg aaaaaatatg taattggcgg
     2101 aacgctactt ttattgatag ttgctggagt aacatttttt ctgactaaaa cgatgatgca
     2161 aatgcaaata gacaaagctg ttcaaataca tataaaagac aaagtacctc tagatgctca
     2221 aattgatagt tccattttaa tatccattat taatgactta caaacaaaaa ttaatgtcaa
     2281 tgatgaatta aagataaagt tagacgaaac atttgatgtg cctttaagaa tgaaccttaa
     2341 agtaccttta aatacagagg ttttcatgga tcaagaatta gatttaaaat ttgatttacc
     2401 tgttgatatc aatcttgatc aaacagaaat gccattgaaa aatttagtaa ttccatttaa
     2461 taaaaaacta caaatcaatg attcattagc agtcgatttt tcaattccat tagacacaaa
     2521 aatcagaaca aactttaagc actttttcaa tatatcctta cctgtaaaag caacgattcc
     2581 tgtcaaagta aacataccag taaaccaacc tttacaagta caagacactc ttgtattaag
     2641 tatgcaagat tatcaaattc ctttgaaaac agttattcct gttgttgcta aagtgcctat
     2701 aaaacaatta gtaaaaatac aaggagaatt agaggttcct gtcgatcaga aaataaaaat
     2761 acctttatca aaagttattt ctacgccagt attacaacca tttacagcca gcgtaaaaac
     2821 tactaacgat ttgcaaacaa gttttaaaag ttcattaaaa gcaaatgctt cttttagcga
     2881 accattgcgt gttattcaaa tggactcttt gcgcatagaa cctagtaaaa ttaaattctc
     2941 tgtgaagtag cattttattt tttttaaata agctgcaaac attcatcagc catttttgcg
     3001 ttgtactcgt tcgctaattc tttattatta atgatatcta tgatggtgcc tattccacaa
     3061 aatccagctg taaaaaagaa tagcaaaccc attaggattt gaccgactaa aaatcgttga
     3121 ataccggcaa caccaaagaa accagcgcaa gcgattataa gcaacaagtc tgattctcta
     3181 cgtttggaat tataaaggat agtgaaactt ttcaattgtt cttcattaag caatgctgtt
     3241 ttatttttca aatacatcat ttcagcaggc gtaatcgatt taatttgtaa gaatggattt
     3301 tccattttgt ctattggttt tataaattgt aaaaaataat tgaacgattc tataaacaat
     3361 catggctaat gcaaaagttc ccagccaatg cgtcgatatc gattgtttat attcgccatg
     3421 tattaataat gcaatggact tgccaattcc acaaccatta caagcagtaa catcaaatag
     3481 cttgaatata caaaaagaaa gatgaggtgc tgttgtgtca ataaaataca aataaaccaa
     3541 agtaattatc caaaagcata attctaagga aaaatatttt tttattcgat gcaatgttgg
     3601 acaattaaac gaagaaatca ggcgttagct cagcaccagg ttttacggca tactgctcta
     3661 aatccgaaat tccttcactg agtaaaactt catcgtcgat aaaaaagttc ccagtagttt
     3721 tcttgccatc tctttttaaa atacaatacg cagcatctgc cataatcgaa ggcttgcgtg
     3781 aggtatttgc tacagattcg ccacctaaca agttaagaac tgccgcagtt gctatagtag
     3841 tacgaggcca aagtgcattc acaccaactt ttcctttgta ttcaccgcct agacctaata
     3901 cgactaagct cattccaaat ttagcaatag aataacctgt atgtcctgca aaccaacgtt
     3961 catttataga agtcaatggc ggcgataaca ttaaaatatg cggattttca gattttaata
     4021 aatacggcat acacattttt gaacacaaga aagtaccacg cgtattaatg tcttgcatta
     4081 agtcataacg tttcatttct aattgttcta atggtgataa atttattgcc gaagcattgt
     4141 taattagaat atcaatacca ccaaatacat cgacagtttg ctttacagca gcttcaactt
     4201 catcgtcatt tcttatatca catttgatgg ctaagccttt tccaccagca ttagtcatat
     4261 cttctgcagc agtataaatt gtgccaccta attttggatt ttccgttgta cttttcgctg
     4321 caattacaat gtttgcacct tctttcgcta aacgcatacc aatggcatgt ccaatgccac
     4381 gcgtcgcacc agaaataaat acagttttat ttttaaatga catattctca gatttaatag
     4441 tgcaatatac aaattaatac agccaaaata ataaccatct attttaactt ctatatttat
     4501 tgctctatgc aattgttaat ttcagtcaac aaaattcagc tacataataa attaactttc
     4561 aatttacaat gcatgcattg taaatcacta tatttacttc gtaaaatata tcaaaatgag
     4621 aaaagtagta tttatagatg gtgtgcgcac acctttttta aaatcaggta ctgattttat
     4681 ggatttaatg tcttatcaat taggtcaatt tgctatcaaa ggtttaatag ataaaacagg
     4741 aattgatgtg aatgaaatcg acaaagttgt atatggtaat gttatctcta atgttagaac
     4801 tgaaaacgtt gctcgtgaag cagcagtaac agcaggcatt cctgtaaaag caccttgtaa
     4861 taccgttaaa caagcttgta tatcagccaa ccaagcaatt gcaaatgcag ttgattccat
     4921 tcaattgggc agagctgatg tcatcgtttc tggtggtaca gaatgtactt cagatacacc
     4981 aattggctac actaaagaaa tgcgtaagaa attattttct gcgcaaaaat taagtacacc
     5041 aatggaatat gcaaaatttg caaccacttt acgtccaacc gattttttac cagatagacc
     5101 atctgtttct gaatttatta caggcagaac aatggggcaa gattgtgaaa tattagcaca
     5161 aaaatttggt gtaacgcgcg aagagcaaga tcaattagca gcagcatcac accaaaacgc
     5221 tgcgaaggct tttgaagctg gtttgatgaa tgatatcgta ccagtacaat tgccacctaa
     5281 cttctcacct attacaaaag ataatggtgt tcgtggcgat tctactgttg aaaaaatggc
     5341 aaaattaaaa cctgctttcg ataaatatgt aggcactatc actgcagcaa atgcttcttt
     5401 cttaacggat ggcgcaacag cagtattatt aagctcagaa gaaaaagcga aagaattagg
     5461 attaacacca aaggcagaaa tcgtagatta tgctttcaca ggtggcgatt tatatgaaga
     5521 attattatta ggaccagcct attcgattgc tgctgttcta aaacaaacag gattaacaat
     5581 tgatgacatc gatgtatttg aaattcatga agcttttgca ggtcaagtat tggcaaattt
     5641 gaatgcatta aaatctgctg aatggtgtaa aaaacaatta ggcttagata aaccaatcgg
     5701 acaaattcct ttgaacaaag taaatgcttg gggtggctct ctttctatcg gacatccatt
     5761 tggtgcaaca ggtggtcgtt tacttacaat ggcagccaat cgtttgcaaa aagatggtgg
     5821 caaatatgct ctattagcag catgtgcagc aggtgcacat ggtcatgcaa tgattattaa
     5881 aaaatattaa tgattagtaa atagcaaaaa cgtaatgcaa tattgcatta cgtttttgta
     5941 taaataaata aactatggta gtgttaaaac gagaagatta tatctctatt gaaaaaaatt
     6001 caaatggcgt atgcaccatt tggatggatc aaaaagatga aaaaataaat aaaatcggcc
     6061 ctgatttaat tggacttttt gaaagtgttt ttgaagagct aaatgctgac gattctatca
     6121 aagcgtttgt attaagtagc aaaaagaaag actttattgc tggtgcggat attgatgcat
     6181 ttgcaaaagt tgaaaaacca ggtgactgga aaccaatagc aagaaaagga catgagattt
     6241 taaaccaaat cgaaaaatca aaaaaaccaa tcgtagcagc aatacatggc aatgcattag
     6301 gtgcgggttt agaaattgcg atggcatgtc atgcgcgtgt tttatcgaat gataaatcta
     6361 caaaagtagc cttacctgaa gtaaaattag gcttactacc tggtggtggc ggcacacaac
     6421 gtttaccgcg tttagtaggc ttgcaagctg cattagatat gatgttaact ggtaaaaatg
     6481 tattcgcata tccagcatta aaaatgggat tagcggacaa ggtggtgcat gtctcagcgc
     6541 tgcaaaaagt agctcagaaa tttgctttgg aattagtaga taatccaata aaacgagaaa
     6601 gaaatgaatt agacattaaa gctggtttcc cttctattca aaagtcattg acaaatattg
     6661 ttttagaagc acccttagtg aataagatag tatttgacca agctaaaaaa atggtggaca
     6721 aacaagcaca tggcaattat cctgcaccat acaagataat ggagtgtgtt gaaattggct
     6781 ggaaccaagg aattgaaaaa ggatatgaag cagaagtaga tagatttgaa gagttgattt
     6841 tatcacctgt gtctcgtcag cttatcaata tcttttttgc aatgacagac aaaaagaaaa
     6901 atccatacgg cgaggacaaa gtaaaacaag tggacagatt aggtatgatt ggcgcaggat
     6961 ttatgggtgc tggtattgct gaagtttcta tgaatgatgg catgcacgtg ctattgaaag
     7021 atatcaatca agacatgatt agctcagcgt ataaaactat ctatgatggt tacaacaaaa
     7081 gagtgtctaa aaaagcaatg tctaaaattg agttagaaga aaaaatggca ttattgtctg
     7141 gctcattaaa ctacgaagac ttagacaatc aagaaatcat tatcgaagct gtgtttgaag
     7201 atttaaaact aaaacaagct atcttaaaag atgtagaagc taacgcaaaa ccaaatacta
     7261 tttttgctac taatacttct gcattaccaa tcaaacacat tgcggcaaat gcaaaacatc
     7321 cggaattggt aatcggtatg cactacttct cgcctgttcc caaaatgcca ttattagaaa
     7381 tcgtaaaaac tgataaaacg gctgattggg taattgcgac ttgctacgat gtaggcgtaa
     7441 gacaaggcaa aaccgttatt gtagtacacg atggtcctgg cttttatact acacgtattt
     7501 tagcgccatt aatgaatgaa gcacaattgt tattagatga aggaggcgat attcaacaaa
     7561 tagatagaga gatgaattta tttggtttcc ctgttggccc aattacatta gcagacgaag
     7621 taggcatcga tgtaggcgct cacattatga gtggcgaatt aatgcaagag ttactttcta
     7681 ctcgaccaaa attcaaggta agtaaaacct tattagaaat atctaaagct ggttacaaag
     7741 gcagaaaaaa taaaaaagga ttttacaaat acgatgaaaa aggcaagaaa gtatcaggac
     7801 aagtagatgc ggatatttac tctttctatg gtggcaacac acgtaaaaaa tttgatgcta
     7861 atgacattca catgcgttgt gcaatggcca tggtaaacga ggctgcatta tgcttacaag
     7921 aaaacatcat cgaaagtcca ttagatggcg acatcggtgc tgtgtttggt cttggcttcc
     7981 ctccttttcg tggcggtcct ttcagatata ttgatagcgt aggtgcacaa aaagtggtag
     8041 atattttaaa tgatttgaca gcacgatttg gtgaacgttt tgagcctgca caaattttat
     8101 tagagtatgc aaaatctggt aaaaagttct atgataaata gatagcaatt cgtcagaatt
     8161 attttaatat ttcaagtata atacgaccca tgggtcgtgt tattttattt taaaacaata
     8221 ctcataacgt tgcaaatcag ctatttatat aaaagaaatg caaaataacc ttaaattaaa
     8281 acaaaatagt ggatattttt gtccattatt aaaatataag gtgtaaattt gtgttctaaa
     8341 aaataaaata gtgttttcta aggcatgtga gtacggaata agagcttcaa tatatattgc
     8401 agaacaatcg ctgcaatgta aaaaagtaag tttaaaagaa gtagcattcg ctatagaatc
     8461 tcctgaagcc tatacttcta aaatattaca acaattagtg cgcgaaagaa taataaaatc
     8521 tattaaagga ccaaatggtg gttttatgat agataatgaa aaaatagaaa cactaaaatt
     8581 gagtgctatt gtattagcca ttgatggcga ttctatttac gaaggttgtg gattaggatt
     8641 aagtagatgc aatgataaaa tgccatgtcc tgtacaccat caatttaaag ccatacgaga
     8701 taatttaaaa gaaatgctaa caagcactac gctgaaatca ttagcactag aagtagaatg
     8761 tggtttgaca tttttaaaaa gataaaacaa atgcaagatt taagaaaaga tattactcat
     8821 ttagacgatg tcaaattgct tgttgatagc ttttatggaa aagtaagaca agacgaaaaa
     8881 ttgaaagaca tttttgacaa cgttattaaa gatagatggc ctgagcatct ggaaaaaatg
     8941 tatcgttttt ggcaaacagt attgcttgat gaacatactt attttggaag tccattcgtg
     9001 ccacacgcca atttgcctgt aattgctgag catttcaacc aatggataca tttatttttt
     9061 gaaacagtag atgaaaattt taaaggaaaa aatgcagaca aagcaaaatg gcaaggacaa
     9121 agaatggctg aaatgtttca tacgaagatt gagtattata aaaacaacac atcaattcct
     9181 ttactataat tattataaaa ttccaattct aaacaaataa ataaaataac atgagtatta
     9241 caaaagaaac aatcattggt gaattagttg caaatgatta ccgcgcggca tctgtattta
     9301 aaaaatacaa attagatttc tgttgtcaag gcaatcgcac cataggtgaa gcgtgcgaac
     9361 taaaaagtgt agatgccaat gctcttgtta gcgatttaat agctacaaca caaaatgtag
     9421 aagctggtgc tattgacttt caaacttggc cattagattt attggctgat tatattcaga
     9481 aaaaacatca tcgttatgtg gaagaaaaat cattagaaat caaaccatat cttgataaaa
     9541 tttgtagagt acacggacaa catcatccag aattatttga gataaatgag cactttacag
     9601 caacagctgg tgagttggcg atgcacatga aaaaagaaga attaatctta tttcctttca
     9661 tcaaaaaaat ggaaaaggca aagcaagaag gaactggctt agaaatgcct cctttcggtt
     9721 ctgtacaaaa tccgattgac atgatgatgc atgagcacac tactgaaggt gagcgtttta
     9781 gaaaaattga agagctaacg aacaattaca atccgccagc agaagcttgc aatacttacc
     9841 gcgttacatt cgctttattg aaagagtttg aagctgattt acacttgcat attcatttag
     9901 aaaataatat actttttcca aaagcaatag aaatggaaaa aacatttaga tgaaaaaaat
     9961 aaaaataaag cggatttatg atgaacccag tagcaatgat ggttatagga tattagtgga
    10021 cagactttgg cctcgtggaa taagcaaaga aaaagctgca atcgacgaat ggaataaaga
    10081 cctcgctcct tctactgaat tgcgtaaatg gtttcatcat gccgctgagt tatttgattt
    10141 attcgaagaa aaatatcgaa acgaattatc ggagcaaaca actgccatta aacacgtaat
    10201 ggaaatcaat aaacatcgtg atatttcctt gctgtatggt gcaaaagatc caaacataaa
    10261 tcacgcgatt gtgctattag atgtaataaa aaataaaatc aacgattatg agcagtccaa
    10321 taaaaagaaa tgaattatta aaaccttata gtagagatca tcatcatggc ttattattgt
    10381 gttggaagat taaagtaggt ttgcaaaaaa atattgaact aaatagaatt agtaattatg
    10441 tatcatcgtt ttggaattct catcttaaag cgcatttttt agaagaagaa aaatatgtat
    10501 ttaccgtttt accatcaaac gatgagcata tcatacaagc aataaaggaa cacaatagtt
    10561 tacgtgccca ttttgaaaaa gaaaatatgg atattgccac cttgcaaagt attgaaatgg
    10621 aattgaatgc acacatacgc ttcgaagaac gcttactctt taacaaaata caagatgcag
    10681 ctacagaaac acaattgcaa caattaaatg agcattataa caatgaatat aaagaagaat
    10741 ggaacgatga attttggttg aattaagtat aaattgtttt ttttgatgga atgtcattta
    10801 caagatgaaa agcacataca aacggcttat aaatctagta aatggcattt ttttataaaa
    10861 aaagaataag tgttgacatt gttaggtatc ctaactatat ttgtattgca atattgcata
    10921 taaagtaaaa ttaaacacaa atgtcaaaat caatttttta tcatgcagga tgtcctgtat
    10981 gcgttagtgc tgagcacgac atcgttaatc taatcggtgc tgaaaatgtc gaagttgtcc
    11041 atatcggtca agacagaagc agaatagaag aagcggaaac aaaaggcgta aaatctgtac
    11101 ctgctttagt aacgccaaac ggaaatgtat tacacatcaa ctttggtgca tcaatggcag
    11161 atgtaaaagg ctaactaatt tcctcactaa attatagtga ggaaatattt ttttcacatg
    11221 aaaaaataaa tatatgcaag tagcagtttg ggacacttat gtcactaaaa aagatggtag
    11281 tataatgcat tttgatatta ttgtgccaga aaatataaaa gatgcagcag tgatctataa
    11341 ttatggccta gaatatttag ccacaaaagg acagcaaggt caaactttga cagcaactga
    11401 atgtcaattt tgccatatcg aaacaataag acctcaatgg aaagatgcaa ttatagaaaa
    11461 aggatattac atttatgaaa tggaaaattg ccattcatga aaaaatctga tttcaattta
    11521 gagcaacaaa atcatcatat tgaaagcaag ataattgcat cattggaacg cattgcacaa
    11581 gcatttagaa tattgttatg gcaagaaagt gtaacgtatg cactcacgcc tattcaaatc
    11641 caattgctaa tttttattca ttaccatcaa atagaaaaat gcaaagtaag ctaccttgcc
    11701 aatgaattca atctttctaa agcaacaatt agcgacacaa taaaattatt ggaacaaaag
    11761 caattcatac aaaaaatatt cgatcaaaac gattctcgaa gctacactat aaaactcact
    11821 aaaaaaggga aagagatagc gttaaaaact tcatttttta gcgaagaaat atcaaagcca
    11881 atcgaacaat tgcacagcga agacaaacaa aatatgttgc ttggcttatt agatattatt
    11941 cgacacttga attcatcagg cgttattaca ttacaaagaa tgtgctacac ttgcacgtat
    12001 tacaagtcga gtcacaatgg caaacaacat tattgtaatt tgttacaaca aaaattatcg
    12061 attgaagatt tacgaataga ttgcccagaa catcaacaat taacatgaac aacattgtaa
    12121 gcaatataca agctattcaa aatcgtatta atgaagcttg tgagaaagca aatcgaaatc
    12181 caaacgaagt aaaactatta ttagcaacaa aaactgtatc tgctgaaaat attaaacatg
    12241 ctatcgaagc aggtgcatgt ataatcggtg aaaacaaaat tcaggaaatt aaagaaaaat
    12301 acgaatcact aaaatcagtt gtacatgaga aacattttat tggtcattta caaagcaata
    12361 aaataaaaga aatattacgt tacgaagtca gttgtttgca atcattggac agattagact
    12421 tagcagaaaa attgcaacaa cgactagcta ctgaaaataa agaattagat gtatatattc
    12481 aagtgaacac atctggcgaa gaaagtaaat ttggtgttgc accaaatgat gctataacct
    12541 tagtaaaatc tgttgctaca ctaaaaaata tacacatcaa aggcttaatg accattggat
    12601 tatttagtgc tgagcataat aaagtaagac aatgttttgc attattgaaa agcattcaac
    12661 aatcgataat acgagaaaat atcgaaggcg tggaaatgaa agaattatct atgggcatga
    12721 gtggcgattt ggaaattgcc attgaagaag gctcaaccat gataagagtt ggcactgcca
    12781 tatttggaaa tcgcatatat cctgatagtt attattggaa tgaatcaaaa gcggttaatt
    12841 aataatcgta catcgaagca ttctatcttt tcctgctaaa tcttttttca gttgtatatc
    12901 aataaaccct ttttgttgca acattgtcac cacttcgttt gcattaaatt catttaattc
    12961 aaaatacaac ttaccatttt tagtcagatg caataaggca aaatctgcaa ttttttcata
    13021 aaaaagaatt ggactgtcat tgctaacaaa taaagcggtt ggtggttcga aattcaaggt
    13081 ggaagaagac attttatctt gttcgaaaaa aagaatataa ggtggattgc ttacgataat
    13141 atctaattgc aaaggtgcag cgtcatctcg ttccatatat aacagaacgt ccacaaaaaa
    13201 attggttgta ggcaagatat catgctgaaa aaattctatt tcagtatggt gcttattagc
    13261 attaatatta gccacttcta atgctttttc actgatatcg attgcaaaaa atttccaatc
    13321 tggtttgttc ttttttagag caatagcaat gcatccacta cctgtaccga tatctaatat
    13381 ttgcaatgaa ttatcagcat gattcgtcaa tatccaatga actaactctt cggtctcggg
    13441 tcgtggaatt aatacattct catctacata aaaatcgagt ccaaaaaaat gtgctttatt
    13501 aaaaatatat tgaattggat aatggttcga tattttctca aaaatatagt tcaactcctc
    13561 tatttcctct tcgtttaaat cgaaatcttt tatatcaaaa taacgcttat gaaaataatc
    13621 ttctagtgca tatttaaaca aatttgcagc ctctcgttca tcaaaagact ctgcaagcat
    13681 cggcaagatt atatttttat actgatgtac gttcaattta ttatttttgt aaagctaata
    13741 ataagtaaga agtattttat atagcattga gtaaagaaac tacatatcat gaaaaatata
    13801 tgcatcgctg tttcgaattg gcgaaactag gcaaaggcta cgtatcgccc aatcctatgg
    13861 ttggtgctgt attagtacac aatgacaata ttattggaga aggttatcat caacaatttg
    13921 gcgaagcgca tgctgaagta aattgcttaa atagtgttgc cgaaaaagat atggaattaa
    13981 tcagcgacgc tactctttat ttgaatttag aaccatgttc acattttggc aaaacgccac
    14041 cctgtacaga tttaattatt cgtcataaaa taaaaaacgt agtcatatcc acgctggatg
    14101 cctctgacaa agtcaacgga aaaggtgtac agcgcctacg cgaacacgac gtgaattgca
    14161 tcactggaat tttagaaaag gaaggcagcg atttaaataa acattttttc tatttccata
    14221 aacacaattt gccttacatc actttaaaat atgcacaaag tcatgatgga tatattggcg
    14281 taattggcaa agaaataaaa ataagtaatg atgctactca gcgctatgta catcaactga
    14341 gagcatcaca tcaggcaatt gccgttggca aaaacacagt attgtcagac aatccttcac
    14401 ttaccgtacg ttttgccaca ggaaaaaatc caatacgtat cgtgcttgga agtgaacatg
    14461 ccattccgaa agactatcat gtatttaata atgaagcaag tacttttttt tatggcagaa
    14521 taaatgacga agcaatcacc atacaagaat tattacaaca attagcaaaa aacaatatta
    14581 tttccttgtt agtagaaggt ggcgcaaatg tgcttcagca atttattgat aataacgctt
    14641 ggaatgaagc gcatatcata actgcgcaaa ataacatgca aataatcagt aatgaaaata
    14701 ttgacaccta tatcaaagca ccagtaattt caggcacttt gcaagaaaac tgcacattac
    14761 aaacagacaa catacaagtt ctaaaaaatc caaatgcttt atcttattct tagtattatt
    14821 tgtgctactg cattagttgt tttatttcgt ttgtttgata aatggaaaat aaactcgaca
    14881 catgccattg tgtttaatta tattttttgt gtgctgacag gattgatttt tgtagatgac
    14941 ttttctgtat ttccaaaaat gatacaatgg aatggttttc ccatcgtcgt gttgttaggt
    15001 tttgcattta ttttaatttt tctcttagtc gggaaaacgg ctttatccct tggtgttgtc
    15061 actgccagta ttgcaatgaa attatcgttt gtcattcctg tcagtgtcgc aattattttg
    15121 tataacgaca gcattactgc gttaaaaatt ataggaatta tatgtgctat cattgctgtt
    15181 tatctgattg catatgaaaa tgaaagtaaa caggaagcct ccacaagagc attgatttat
    15241 ccatttttaa tttttattgg aagtggcttg tgtgatacag catttaatta cattcaaaag
    15301 aaaatgatgc caacaaattg ggatcatcaa gtaacaatag gtgtttttgc agcagctatg
    15361 gttttaggta tcttattaca tttcagagat aaaaaattgt atcaatggaa gaatgtatta
    15421 gcaggaatta ttttaggtat tccaaattat ttctctttat attttctttt aaaaacattg
    15481 agcacattgc cttggcaaag ctctgtcatt tttcctgtca ataatttagg aattgtttgt
    15541 ttgtctgctt ttattggatt cattatttac aaagaagcgt ttaacacacg aaaatcaatt
    15601 ggatttatac tcgcaatcag cagtatcatt attattggat ttattgcata atatgtatag
    15661 ctattacaca atacataatg ctgttgagca agtgctgtac aaagacaaag ccagcaaatt
    15721 ttatggcttc gcttatcctg taaaaaatga aatagaaata aaagaaaaac tggaatttgt
    15781 gaaggcacaa catccgaaag caacgcatca ttgttacgct tatcgaatag gtattgataa
    15841 aaataactat agagccaatg atgatggcga accgagtggc acagctggaa agccgatatt
    15901 aggacaga
//
LOCUS       DAOQQV010000058        15540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1214_length_15540_cov_6.579077, whole genome shotgun sequence.
ACCESSION   DAOQQV010000058 DAOQQV010000000
VERSION     DAOQQV010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15540
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_15540_cov_6.579077"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="PLL59_07345"
     CDS             <1..1647
                     /locus_tag="PLL59_07345"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HMY23659.1"
                     /translation="QYATHPSYKAPRGNILHAKSWQTEAPLRMLLNNLDFEVAENPAE
                     LVVYGGIGQAARNVEALNKIIKLLLELDENHSLLIQSGKPVGIVRTHPQAPRVLIANS
                     NLVPKWATWEHFYELREKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVECGRQHFAGN
                     LKGKLLVSSGIGGMGGAQPLAAVMAGATYLGADINKASIQKRLDTKYIDFMYDNYEEA
                     RNHAICARDKGEAISIGVVMDAGDLLQHLLDDNIIPDILSDQTSAHDPLNGYVPHDIS
                     FDEALELRKTNPTLYKQKAIKSMALHVQLMLDMQKRGAITFDYGNNIRAFAQEGGVQN
                     AFDFPGFTPAYIRPLFCEGKGPFRWVALSGDPQDIYTTDQALMELFPENKAMINWLQQ
                     AQQRISFQGLPARICWLGLGEREKAGLLFNDLVRSGKVKAPIVIGRDHLDCGSVASPN
                     RETEAMKDGSDAVSDWALLNLLSNASGGATWVSFHHGGGVGIGYSQHAGMVVLADGTE
                     RADECLRRVLFNDPAMGIFRHADAGYEIAQNIGEKHGIII"
     gene            1703..2176
                     /locus_tag="PLL59_07350"
     CDS             1703..2176
                     /locus_tag="PLL59_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HMY23660.1"
                     /translation="MAKYKSNHKEPLLLNEPLMVYGAKAIDVTQFFQNTYTVNNTIEK
                     GVSITLFDDIQRLVQFSEEKLADILSLSTKSIQRYRKEKGFRFKPIHSEKLIELAEVA
                     TFGLKVFDSKDQFHAWLDTPSFALANNKPADLLHNSYGIKLVMEELNRIEHGIFI"
     gene            2186..2644
                     /locus_tag="PLL59_07355"
     CDS             2186..2644
                     /locus_tag="PLL59_07355"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HMY23661.1"
                     /translation="MLVFRLVKQQYSKVLSGKGAAIFGERWNSKGLELIYTVENRSLA
                     MAEVAVHFSLATMPSSYNMLSIFIPDNLTIKEIKTIELPDDWNTFPHAKSTQNIGDNF
                     ILENKFVTLKVPSVVTKGDFNILINPHHKDFKKIKIKSSEPFLFDRRIFR"
     gene            2650..3882
                     /gene="hutI"
                     /locus_tag="PLL59_07360"
     CDS             2650..3882
                     /gene="hutI"
                     /locus_tag="PLL59_07360"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HMY23662.1"
                     /translation="MLITNIKYLFNVREATHLLRGHALAELPTLENAFLKIKDGYIED
                     YGSIHTLEEEDFKQEIYDAKQSSILPCWCDSHTHLVFAATRENEFIDKIKGMSYAEIA
                     AKGGGILNSAKKINETPEDTLYLHAQARLAELIQLGTGAIEIKSGYGLSVEGELKILR
                     VIKKLKEQNIIPIKATFLGAHAIPNAYKNNRSAYIDIIIHEMLPKIAAENLADYIDVF
                     CEDGFFTPKETIQICKAGMQYGLQAKIHANQLNISGGVQAGISVNAISVDHLETIGEE
                     EIALLAKETAPIATLLPSCSFFLNMHYAPARTLIENNAAIALASDYNPGSSPSGNMNF
                     VVSLACTQMKMLPEEAINAATINGAFALEVEHLVGSITRGKKANFIITKNIPSLAFLP
                     YQFGTNNINTVVINGNIL"
     gene            4006..4713
                     /locus_tag="PLL59_07365"
     CDS             4006..4713
                     /locus_tag="PLL59_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23663.1"
                     /translation="MSILGEDTKQKGSIDRSRSNLLRDLEQPTLLFLCRVMPRFLTPD
                     ILTAIGMLGTLLIFAAFYMAHSLNNKIYLLFAVLGFAINWFGDSLDGRIAYYRNIPRK
                     WYGFALDCVMDWISLVFMSAGFFLYIEPDFQIYVFLFACNYGWVVLMAQLKYKITDKY
                     SIDPGPIGPTEVRILLCLVIIAEWYFGNVLIYFSTIGNIVFLIINLVDTYKLLQFGNA
                     RDAEEKRLKAEAQSKNA"
     assembly_gap    4823..4922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4923..5249
                     /locus_tag="PLL59_07370"
     CDS             <4923..5249
                     /locus_tag="PLL59_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HMY23664.1"
                     /translation="VINFSINKYWTYQSKKPSLTSQLIKFYIVVAGSIYLKSNGTYIL
                     THLLQIDYKITRIMVDLVVSIGFNFMLQKYWVFRTTTLTESINQYSDTQKEKNSTKQQ
                     HEYQEV"
     gene            5283..6311
                     /locus_tag="PLL59_07375"
     CDS             5283..6311
                     /locus_tag="PLL59_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HMY23665.1"
                     /translation="MQNFVFYKKENAFAFIKKRNGETKLGEKIRAISNQKNWKNELKK
                     SSAPFVIIGIPEDIGVKANFGVGGAHTVWKPFLQAFFNIHDNQFLSGDSFLLLGEFDF
                     SDLQQKIKNKPIEFVREQVEVIDKQVAQIIQAITEAGKTPIIIGGGHNNAYGNIKGTA
                     LGLQQNGKIKNAKINAINIDAHADFRALEGRHSGNGFSYAMHDGYLDKYSIIGLHENY
                     TSQAMLENLNKNKKIQTHFFEDICIRKKIEFDDVIQDAILFTKNSYTGIEIDIDAIEN
                     TLSSAISPCGFSSQQIRQFIHQVASKSKIAYLHICEGATTLDNGLQSTTTGKLIAYIV
                     SDFAKSIR"
     gene            complement(6322..6849)
                     /locus_tag="PLL59_07380"
     CDS             complement(6322..6849)
                     /locus_tag="PLL59_07380"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HMY23666.1"
                     /translation="MPFDFHSLLDIALRSSAVYVFMLVAFRIFGKRELSQLSIADLVL
                     IVLISNAVQNAMVGDNTSLIGGLVAATTLFLLNLVLSFLMFKFKSLRVLVQSEPITLI
                     FKGKVLEKNLKAVMISEEELLSAIREHGIENVADVSLSMLEPDGNISVISGNDTHLKR
                     TQYKRKKHHKTLQDI"
     gene            7010..9457
                     /gene="pheT"
                     /locus_tag="PLL59_07385"
     CDS             7010..9457
                     /gene="pheT"
                     /locus_tag="PLL59_07385"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMY23667.1"
                     /translation="MKISLNWLKSYLNIDISIDELQHLLTDIGLEVESIEPFESIKGG
                     LKGLVVGHVVEREKHADADKLSVCKVDVGSETLQIVCGAPNVAAGQKVIVAQVGTKVF
                     PLSGEAFEIKRAKIRGVESAGMICAEDEIGLGTSHDGILILENNTQVGTLAADLFEVE
                     NDHVIEIGLTPNRTDAFSHIGVARDLAAALTFRKKQDFTLQLPRCATLGKEKNTEFTV
                     TVENTKACPKYYGVIINNIKVEASPIWLKNRLKTIGVKPINNIVDITNFVLHEYGQPL
                     HAFDLSAIKGNGIIVKNVAAKTKFIALDNTEIALDEEDLMICDSEGNSMCIAGVYGGA
                     NSGVKNDTTAIFLESAYFDPKTIRRTSTRHGLRTDAATHFEKGIDPNISERALLRAVH
                     LISEIANSEVASEIYTIENKTFEHFPVSLTYDNVKRLTGADISRDDINKILSLLEIKI
                     QKANDEQADLLVPPYRTDVTREADVIEDILRIYGFNNIVIPKKINASISFSSAFDSHK
                     LYNQTADALVNFGFFEMMNNSITKSKYQEKLTQQSPENWVRLLSSINAELDVLRNNML
                     FTGLESVAHNINHKNTNVKLFEFGKTYSKAPSDDINTSNYTEINHLSIFVSGNKSAAN
                     WNTVAKKTDFYYIKSIVDSILSKIGISAFDSYDNDASEIFQYALSYKKGEKKLVEFGK
                     IHSTYTKLFDIKQDVFFIDFNWDRITQLARKNNVSYSEIPKYPSIKRDLALLLDKKIK
                     FDEVKAIATKFGKKILRNIELFDIYEDEKIGKENKSYAVSFTFRDDTKTLQDTDIESI
                     MKKLIQEYQTQLNAVIR"
     gene            9459..9749
                     /locus_tag="PLL59_07390"
     CDS             9459..9749
                     /locus_tag="PLL59_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23668.1"
                     /translation="MAKIDEKWARIFKKIQLILEKNQKLEDANNALQLKITELEKNIT
                     EQNKEKESLKEQIQYIKAAKEVHLSEKERAVTKQQLKEFMIEIDDCLAKLIQ"
     gene            9749..10045
                     /locus_tag="PLL59_07395"
     CDS             9749..10045
                     /locus_tag="PLL59_07395"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HMY23669.1"
                     /translation="MAEPLNINLLIAGRPYKLKVTPDEEGYVRQAAKDINDKIAEYQK
                     TLLTRDRQDFLSMIALQSTAEALQQKNKATNANIDAKLDELEALLDKQLGITLF"
     gene            10235..11830
                     /gene="rny"
                     /locus_tag="PLL59_07400"
     CDS             10235..11830
                     /gene="rny"
                     /locus_tag="PLL59_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HMY23670.1"
                     /translation="MIAIIVGVVALLLGFGIGKILSKANVEKQLAEAKLAEEKAKALE
                     EKSKTEAEHIRKEAYATAESLKKDKMLEAKEHFFRLKEEHNKEIEQRNRKIIEAENKA
                     KQTEQSLRDKLSNAGKQEKENEILKGKLNEQIELANIKRSELEKAHTEHIAKLEQVAK
                     LSAEDAKAQLVEQLKSKAQSDAMAHVKQVMDEAKLKANKEAKKIIIQSIQRTAAETAI
                     ENTVSVFNLSSDEQKGQIIGREGRNIRALEAATGVEIIVDDTPEAIIISGYDAYKREI
                     ARLSLQRLVTDGRIHPARIEEVVAKTEKQLEQQIMEIGERTVIDLGIHGLHPELIRLV
                     GKMRFRSSYGQNLLQHSREVANLCAIMASELGLNSKIAKRAGLLHDIGKVSDEDPELS
                     HALLGAKLCEKFGESPVIVNAVGAHHDEMEMMYLVSPIIQACDAISGARPGARREIME
                     SYMKRIDELEKLAVSYQGVQKAYAIQAGRELRVLVEAEKVDDKQVEEISFKIAQQIQD
                     EMTYPGQIKVTVIRESRSVSVAK"
     gene            complement(11864..13048)
                     /gene="mqnE"
                     /locus_tag="PLL59_07405"
     CDS             complement(11864..13048)
                     /gene="mqnE"
                     /locus_tag="PLL59_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HMY23671.1"
                     /translation="MNTSADSIYTILQNLQIDHVLKQIAEKVLQNERITFDEGVLLYE
                     KGELGFVGMLANYIRNKKHGNNTYFNRNFHVEPTNICVYTCKFCSYSRLIKNREQGWE
                     YSIDDIINIVKKYDGQPVTEVHITGGVIPKQDFNFYIDLFKAIKAHRPDLHIKALTPV
                     EYHYILKKAKMSYEDGMRAMKAAGLDSMPGGGAEIFDAAIRDEIAGGKCSAEEWLAIH
                     EVAHQLGIPSNATMLYGHIEKYEHRIDHMERLRQLQDRTNGFNAFIPLKFRNQHNEMK
                     HLPEATITEDLRNYAISRIYLDNFEHIKSYWAMIGRNTTQLSLNFGVDDVDGTIDDTT
                     KIYAMAGSEEQHPAMSTRELVHLIKAVGRRPLERDTVYNVLQDYSNVVFEEDEKEKAR
                     MY"
     gene            13139..14425
                     /locus_tag="PLL59_07410"
     CDS             13139..14425
                     /locus_tag="PLL59_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMY23672.1"
                     /translation="MKNEKSILYLLTFIWFCAIVDFLLMLPLGTEMMEIYNIQPKEYS
                     YLIFAFSLSGGLSSFLASFYIDSYDRKTILLCAFFFFAIGSILCSFAHTYELMLFSRF
                     FTGIFGGLLGGVSTAYVSDMVPYERRGNAMGILNLGFGLASILGIPFAIFICEHLDVF
                     WPFRIIGILSLVVLVPAYFFLPSMRNHIPEDNTTIHDTFKAIFSLAIYPFTFYFFPRF
                     SQQLKQNIQKTKEVFDVLKNRNLQRALSFAFFLVLGHFMFISFINPYLVGNLGFKLED
                     TKWMYIIGGLSVSLTSPRIGKFIDTLGKVKSFRILIILSFFPILIIAHLQQASIFMAL
                     LICAFMFIFNSGRMIAAMTLITGAPTEEQRGKFLVIRSSLIEMSEGASALIGGMILTK
                     DELTGHLNNYHYIAYLAVLIGLLCIYLASKIEINEE"
     gene            14505..15251
                     /locus_tag="PLL59_07415"
     CDS             14505..15251
                     /locus_tag="PLL59_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23673.1"
                     /translation="MNVCISNAQNISGRWIGKRTSGASLTLDIQQCKDSIFGTAYIIF
                     PEEIGSANVAFSGNIQNGILEYETTKILEQNVDSAVLLCLVTGRDILKIKKRKQILEG
                     ACISIDKKEPCFNLSCIERYEKKMEFTFHKNLFLNKKVLIVDTIWASQDSLDIKVWDN
                     LKEDGDIVSIYLNEHNILEKYTLKHQQKTIKLACTKTYNNIVFYAHNVGSEAPNTASI
                     GIYQNGKLLKEIVLKSDAEKSESFVILRSN"
     gene            complement(15248..>15540)
                     /locus_tag="PLL59_07420"
     CDS             complement(15248..>15540)
                     /locus_tag="PLL59_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HMY23674.1"
                     /translation="GLSKDKYTVTFQSRLGKEIWLEPYTVDVIPQLGKAGKKKLLIFS
                     PAFVADCLETLYEIRVEYLELFEQYGGEHITLVESLNSEDNWVDAVCDIVTT"
BASE COUNT         5472 a         2611 c         2747 g         4610 t
ORIGIN      
        1 caatacgcaa cacatccatc atataaagca ccacgtggaa atatacttca tgctaaatct
       61 tggcaaacag aagcaccact gcgaatgcta ctaaataatt tagatttcga agttgctgaa
      121 aatccagcgg aattagttgt gtatggtggt attggacaag cagctcgcaa tgtagaagca
      181 ttaaataaaa tcattaaact tttgttagaa ttagatgaga atcattcttt gttaatacaa
      241 agtggcaaac ctgttggcat cgtgcgcaca catccacaag caccacgtgt gttgattgcc
      301 aattctaatt tagtacctaa atgggcaacc tgggaacatt tctatgaatt gcgtgaaaaa
      361 ggtttgatga tgtatgggca aatgactgct ggttcttgga tttatattgg ttctcaagga
      421 attgttcaag gtacctatga aacatttgta gagtgtggtc ggcaacattt tgctgggaat
      481 ttgaaaggaa aattattagt cagttcaggc ataggcggca tgggtggcgc acaaccatta
      541 gctgccgtga tggctggtgc tacttatctt ggtgctgata tcaataaagc tagtatccaa
      601 aaaagattag atacaaagta catagatttt atgtacgaca actatgagga agcacgtaat
      661 catgctattt gtgcaagaga taaaggggaa gctatttcta ttggtgtggt aatggatgca
      721 ggcgatttgt tacagcattt attagacgac aatattatac cagatatttt atcagaccaa
      781 acatctgctc atgatccatt gaatggctat gttccgcacg atatttcttt tgatgaagca
      841 ttagagttgc gcaaaaccaa tccaacttta tataagcaaa aagcaattaa aagtatggca
      901 ctccacgtgc aattgatgtt ggatatgcaa aaacgtggcg ctataacttt cgattatggc
      961 aataatatcc gtgcgtttgc acaagaagga ggtgtgcaaa atgcatttga ttttccagga
     1021 tttacaccag catatattcg tccattattt tgcgaaggca aaggtccttt tcgttgggta
     1081 gcattaagtg gcgatccaca agatatatac acaacagatc aagcgttaat ggaattattt
     1141 ccggaaaaca aagcaatgat aaactggcta caacaggcac aacagcgcat ttcatttcaa
     1201 ggcttacctg ctagaatttg ctggttagga ttaggcgaac gagaaaaagc tggtttgttg
     1261 tttaatgact tagtgcgctc tggaaaagtg aaagctccga ttgttattgg acgcgaccat
     1321 ttagattgtg gtagtgtagc atcgccgaat agagaaacag aagcgatgaa ggacggtagt
     1381 gatgctgtta gcgattgggc attgttaaat ttattgagca atgcaagtgg tggcgccact
     1441 tgggtgagtt ttcatcatgg tggtggtgtc ggaataggat attcacaaca cgctggaatg
     1501 gttgttttag ctgatggcac tgaacgtgca gatgaatgtt taagacgtgt tttatttaac
     1561 gatcctgcga tgggaatttt tagacatgca gatgctggat atgaaattgc tcaaaatatt
     1621 ggagaaaaac atggaataat aatttagaaa aacttgacaa actaagacaa aagtattaaa
     1681 tttgtggaca aaagtcttag gaatggcaaa atacaaatct aaccataaag aacctttatt
     1741 gctaaatgaa ccactcatgg tatatggtgc aaaagcaatc gatgtaactc aattttttca
     1801 aaatacatat acagtaaata acaccataga aaaaggagtt agcatcacgc tctttgacga
     1861 catacaaaga ttagttcaat tttcagaaga gaagttagca gatattttat cgctttccac
     1921 aaaatctata caacgatatc gaaaagaaaa aggatttcgt tttaaaccta tacattcaga
     1981 aaaattgatt gaattagcag aggttgccac atttggatta aaagtttttg actcaaaaga
     2041 ccaatttcat gcatggctag atacgccaag ctttgcactg gctaataata aaccagctga
     2101 tttactccat aattcttacg gaattaaatt agtcatggaa gaacttaatc gcattgaaca
     2161 cggcattttt atataaatag cattcatgct tgtatttcga ttagtaaaac aacaatacag
     2221 caaagtactc tcaggaaaag gtgctgctat atttggtgag cgatggaatt ccaaaggact
     2281 agaattgatt tacactgtcg aaaatcgttc gctggcaatg gcagaagtag cagtacattt
     2341 cagtttggct acaatgcctt cttcctataa tatgttaagc atttttatac cagacaattt
     2401 aactattaaa gaaataaaaa caattgagct tccagacgac tggaatactt ttccgcatgc
     2461 aaagtccaca caaaacattg gggacaactt catcttagaa aataaatttg taacacttaa
     2521 agtaccttct gtagtaacta aaggcgattt caatatttta ataaatccac atcataaaga
     2581 ttttaaaaaa ataaaaatca aatcttctga accttttcta ttcgacagac gtatatttag
     2641 ataatcatta tgctcattac caatataaaa tacttgttca atgttcgtga agcaacacat
     2701 ttattgcgtg ggcatgcatt agcggaatta ccaacattag aaaatgcatt tttaaaaatt
     2761 aaagatggct acattgagga ttatggctcg atacatacac ttgaagagga agattttaaa
     2821 caagaaattt acgatgcaaa acaatcatca atcttaccat gttggtgcga cagccataca
     2881 catcttgttt ttgctgctac tcgtgaaaat gaatttatcg ataaaataaa aggaatgagc
     2941 tatgcagaaa ttgctgctaa aggtggtggc attttaaact ctgcaaaaaa aataaatgaa
     3001 acgccagaag atacattata cttacatgcg caagcacgat tagctgagct catacagctt
     3061 ggcactggtg caatagaaat aaaaagtggc tatggtttaa gtgtagaagg cgaattaaaa
     3121 atactacgcg taataaaaaa attaaaggaa caaaatataa ttccaatcaa agctacattc
     3181 ttgggcgcac atgctatacc taatgcgtat aaaaataata gaagtgccta cattgatatt
     3241 attattcatg agatgcttcc caaaattgca gcagaaaatc tagctgatta tatagatgtt
     3301 ttttgtgaag atggattttt tacaccaaaa gaaacgattc aaatttgcaa ggcaggaatg
     3361 caatatggct tacaagcaaa aatacatgct aaccaattaa acatttcagg cggcgtacaa
     3421 gctggaatta gtgttaatgc catttctgta gatcatttag aaacaattgg cgaggaagaa
     3481 attgcattat tagcaaaaga aacagctcct attgcaacgc ttttaccgag ttgttctttc
     3541 tttttgaata tgcattatgc acctgcacga acattaatag aaaacaatgc tgccattgcc
     3601 ttagcatctg attataatcc tggctcctcg ccttctggaa atatgaattt tgtggtttct
     3661 ttagcatgta ctcaaatgaa aatgctacca gaagaagcga taaatgctgc caccataaat
     3721 ggtgcttttg ccttggaagt agaacatctt gttggcagta tcacacgtgg taaaaaagct
     3781 aactttatta ttacaaaaaa cattccgtca ttagcttttt tgccatatca atttggaacg
     3841 aataacatta atacggtcgt gataaatggt aatattttgt aagtaaccaa ttataggtat
     3901 tttaaataaa ataattgggt tttgaatata atgtgaatat aatttgactt cagtttaacg
     3961 aacatctaac attaatacac taattttgtg gactatttta gtcctatgag tatattaggt
     4021 gaagacacaa aacaaaaagg ttcaatagat agaagtagat ctaatctatt acgtgacctt
     4081 gaacaaccaa cgctcttatt tttatgccgt gtaatgccaa ggtttttaac gcctgacata
     4141 cttacggcta ttggaatgct gggcacctta ttaatttttg ctgcatttta catggcacat
     4201 agcttaaaca ataaaatcta tttattgttt gcggtgctag gatttgccat aaactggttc
     4261 ggcgactcct tagatggtag aattgcctat tatcgtaata ttcctagaaa atggtatggc
     4321 tttgcactgg attgtgtaat ggattggatt tcattagtat ttatgagtgc aggatttttc
     4381 ttgtatatcg agccagattt tcaaatttat gtattcctat ttgcttgtaa ttatggttgg
     4441 gttgttttga tggctcaatt aaaatacaaa attacggata aatactctat tgatccaggc
     4501 ccaattggtc caacagaagt gcgaatattg ctttgtttgg tcattattgc tgaatggtat
     4561 tttggtaatg ttttaattta tttctctacc ataggaaaca ttgtattctt aatcattaat
     4621 cttgtggata cgtataaatt attacaattc ggtaatgctc gtgatgcaga agaaaaaaga
     4681 ttaaaagcag aagctcaatc aaaaaatgct taagaaaaaa tcattactcg tattcggaaa
     4741 agcccaagtc tctgctttta taggtggctt agtcgattat ggtgtaatgg tattttgcgt
     4801 ggaattatta tttatcaact tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nngttattaa tttttctata aacaaatatt ggacatacca atcaaagaaa ccatcgctaa
     4981 ccagccaatt aataaaattc tacattgttg ttgctggtag catctattta aaatccaatg
     5041 gaacctacat tttgacacat ttattacaaa ttgattataa aatcactcgt atcatggtag
     5101 atttagtcgt gtcgattgga tttaatttca tgctacaaaa atattgggtt ttcagaacta
     5161 ctacattaac agaaagcatt aaccaatatt ctgacacaca gaaagaaaaa aacagcacaa
     5221 agcaacaaca cgaataccaa gaagtataaa ctactattca gctttttatt tacctttaag
     5281 ttatgcaaaa ctttgttttt tacaaaaaag aaaacgcatt tgcttttata aaaaaaagaa
     5341 atggtgaaac taaactcggt gaaaaaatcc gagctatctc aaatcaaaag aattggaaaa
     5401 atgaattaaa gaagtcgagt gcaccatttg taattattgg tatcccagaa gacattggcg
     5461 taaaagcaaa ctttggagtt ggtggtgctc atactgtttg gaaacctttt ttacaagctt
     5521 tcttcaacat tcatgataat cagtttttaa gtggcgattc atttttatta ttaggcgaat
     5581 tcgacttttc agaccttcaa caaaagataa aaaataaacc aatagagttt gtacgggaac
     5641 aagtggaagt catagacaaa caggtcgcac aaattataca agcaattact gaagctggca
     5701 aaactccaat aatcattggt ggtggacaca ataatgctta cggcaacatc aaaggcactg
     5761 ctcttggatt acagcaaaat ggaaaaataa aaaatgcaaa aatcaatgca ataaatattg
     5821 atgcacatgc tgattttcgc gcattggaag gcaggcattc tggcaacggt ttttcatatg
     5881 ccatgcatga tggctatctt gacaaataca gcatcattgg tcttcatgag aattatactt
     5941 cgcaagcgat gctagaaaac ttgaataaaa acaagaaaat acaaacacac ttttttgaag
     6001 atatttgtat aagaaaaaaa atagaatttg acgacgtgat tcaagatgct attttattta
     6061 caaaaaatag ctacacagga atcgaaattg atatagatgc aattgaaaat acgctttcga
     6121 gtgccatttc tccatgtgga ttttcttcac aacaaatacg acaatttata catcaagttg
     6181 catcgaaatc taaaattgct tatctgcaca tttgcgaagg tgcaacaact ttagacaatg
     6241 gtttacaatc gacaactact gggaaattaa tagcttatat cgtcagtgat tttgcaaaat
     6301 ctatacgata gcctgtaata ctcagatatc ttgcaatgtt ttgtggtgct ttttgcgttt
     6361 atactgcgtc ctttttaaat gtgtatcatt acctgaaatc acgctaatat ttccatctgg
     6421 ctcgagcatg gataagctta catccgctac gttttcaata ccatgttctc taatagcaga
     6481 caataattcc tcttcagaaa tcatcacagc tttcaggttt ttttctaaca ctttaccttt
     6541 aaaaattaaa gtaataggtt cggattgcac taacacacgt aagcttttaa atttaaacat
     6601 caaaaaactc aacactaaat tcaacaaaaa caatgtagta gcagctacca aaccgcctat
     6661 aaggcttgta ttatcaccaa ccatagcatt ttgcaccgca ttactaatga gcacaatcaa
     6721 cactaaatct gcaatagaca attgagataa ttcgcgcttg ccaaatatgc gaaatgcgac
     6781 aagcataaac acatatacag cactacttct taatgcaata tctaaaagtg aatgaaaatc
     6841 aaaaggcata ttcaaagtta ataaatatta ctacaaattc aatttaagat ataaatgaaa
     6901 aattatgttg taaaatgaaa aaaaatcaac aatgatgaca ttcaattcat agcattcctt
     6961 taatctataa acaattatag gcaatatttt ttttgtattt ttgtcgatta tgaaaatttc
     7021 acttaactgg ctaaaatctt acttaaacat tgacatatca atcgatgaat tacagcattt
     7081 attaacagat attggtttag aagtagaaag cattgagcca tttgaatcca taaagggtgg
     7141 cttaaaaggc ttagtagttg gtcatgttgt ggaacgagaa aaacatgctg atgccgataa
     7201 attatctgtt tgcaaggtag atgttggaag tgaaacctta caaattgttt gcggcgcgcc
     7261 aaatgttgct gctggacaaa aagtaattgt agcacaggta ggcacgaaag tatttccatt
     7321 aagtggcgaa gcattcgaaa tcaaaagagc aaaaattaga ggcgtcgaat cagcaggaat
     7381 gatatgcgct gaagacgaaa taggattagg cacttcgcat gatggcattc tgatattaga
     7441 aaacaacaca caagtaggca cattagcagc ggatttattt gaagtagaaa atgaccatgt
     7501 aatagaaatc ggattgacac ctaaccgcac agatgcattt tcacatatcg gtgtggcgcg
     7561 cgacttagct gccgcattga cattcagaaa aaaacaagat tttacattac aattaccaag
     7621 atgcgcaaca ttaggcaagg aaaaaaatac tgaatttaca gtcacagtag aaaacactaa
     7681 agcttgtcca aaatattatg gtgtcatcat caacaacatc aaagtagaag cgtcgccaat
     7741 ttggttaaaa aacagactaa aaactattgg cgtaaaacca ataaacaata tcgtagatat
     7801 taccaacttc gtattacacg agtacggcca gccacttcat gcttttgatt tatcagcaat
     7861 aaaaggcaat ggcataattg tgaaaaatgt agctgcaaaa acaaaattta ttgcgcttga
     7921 taatacagaa attgcactcg atgaagaaga cctgatgatt tgtgatagcg aaggaaacag
     7981 catgtgtatt gctggtgtgt atggtggcgc aaattctggt gtaaaaaatg atacaacagc
     8041 catcttttta gaaagtgcct atttcgaccc aaaaacaata agacgcacct ctacacgaca
     8101 tggcttgcga accgatgcgg ctacacattt tgaaaaaggc atagatccaa atataagcga
     8161 acgtgcactg ttacgcgctg tgcacttaat ttctgaaata gcgaatagcg aagtcgcttc
     8221 tgaaatctat actatcgaga acaaaacatt cgaacatttt ccagtaagtt taacttatga
     8281 caatgtgaaa cgtttgactg gtgccgatat cagcagagac gatatcaata aaatattatc
     8341 gctcttagaa attaaaatac aaaaagcaaa cgacgaacag gcagacttgt tagtgccgcc
     8401 atatcgcact gatgtcacgc gcgaagcaga tgttatcgaa gatattttac gcatttatgg
     8461 ttttaataat atcgtaattc ctaaaaaaat aaatgcaagt atcagctttt catcagcatt
     8521 cgacagccat aaattataca atcaaaccgc agatgcatta gtaaattttg gattttttga
     8581 aatgatgaat aacagcatca ccaaatctaa atatcaagaa aaacttacgc aacaatcgcc
     8641 agagaattgg gtgcgattgt taagttcaat caatgcagaa ttagatgtgt tgcgaaacaa
     8701 tatgcttttt actggattag aatctgtggc gcacaacatc aaccataaaa atacaaatgt
     8761 aaaattattt gagttcggca aaacgtactc aaaagctcca agtgatgaca tcaataccag
     8821 caattacaca gaaataaacc atttgtccat ttttgtatct ggcaataaaa gtgctgcaaa
     8881 ctggaataca gtagctaaga aaacagactt ttattatatc aaatctattg tcgattcaat
     8941 tttatcaaaa attggcattt ctgcatttga cagctatgac aatgatgcga gtgaaatatt
     9001 tcaatatgca ttaagctata aaaaaggcga gaaaaaatta gttgagtttg gcaaaataca
     9061 cagcacatat acgaagttat tcgacatcaa gcaagatgta ttttttatag atttcaattg
     9121 ggacagaata acacaattgg caagaaaaaa taatgtttcc tattcagaaa taccaaaata
     9181 tccatctatc aaacgcgatt tagcattatt attagacaaa aaaataaaat ttgatgaggt
     9241 aaaagccatt gcaacgaaat ttggtaagaa aattttgcgt aatattgagt tgtttgatat
     9301 ctatgaagat gaaaaaatag gaaaagaaaa taaatcctat gcggtaagtt tcacctttag
     9361 agatgacaca aaaacacttc aagatactga tattgagagt attatgaaga aattaattca
     9421 agaatatcaa acacaattaa acgctgttat acgttaatat ggcaaagatt gatgaaaaat
     9481 gggctcgaat ttttaaaaaa attcaactaa ttttagaaaa aaatcaaaag ttggaagatg
     9541 caaacaatgc cttacagctc aagattactg aattagaaaa aaatattacg gaacaaaaca
     9601 aagaaaaaga aagtttaaaa gaacaaatac aatatataaa agcagccaaa gaggtgcatt
     9661 tatctgaaaa agaaagagca gtaaccaagc aacagttgaa agagtttatg atagaaattg
     9721 atgactgttt ggcaaaattg atacaataat ggcagaacca ctcaacataa atctgttaat
     9781 agctggaaga ccttacaaat taaaagtaac accagatgaa gaaggctatg ttcgacaggc
     9841 cgccaaagat atcaatgaca aaatcgccga atatcaaaaa acattgctca cgcgcgaccg
     9901 acaggatttc ctgtcgatga ttgccttgca gtcgaccgca gaagcattgc aacagaaaaa
     9961 caaagcaaca aatgccaata ttgatgccaa attagatgaa ctcgaggcat tactcgacaa
    10021 acaactcggc atcactctct tttaatttct gagttatcct tacgctgtgt aaaacataga
    10081 aaatgcacat tttatatttt gtgtattttc acttgtcaac tgttttttta ttcataaaat
    10141 acattgacat acaaatggtt ttgattgctt tccatttcaa aaagcaacag aaatattttt
    10201 aaacaattaa aacaacaaca cagtggatcc aatcatgata gcaattatag taggcgttgt
    10261 cgctcttctt cttggttttg gtataggaaa aatactttcg aaagccaatg tagaaaaaca
    10321 acttgccgaa gcaaaattag cagaagaaaa agctaaagca ctagaagaaa aatcaaaaac
    10381 agaagcagaa cacattcgca aagaagcata tgcaactgcc gaatctttga aaaaagataa
    10441 aatgctcgaa gccaaagaac atttttttcg cttaaaagaa gaacataata aagaaattga
    10501 acaacgcaac cggaaaataa ttgaagcgga aaacaaagca aagcagacag aacaatcatt
    10561 gcgagacaaa ctatcgaatg ctggcaaaca agaaaaagaa aatgaaatat taaaaggcaa
    10621 gcttaacgaa caaatcgaat tagcaaatat aaaacgtagc gagcttgaaa aagcacatac
    10681 agaacatatt gcaaaattag agcaagtagc caagctttct gctgaagatg ctaaagcgca
    10741 attagtagag cagctaaaaa gcaaagctca atctgatgct atggctcatg taaaacaagt
    10801 gatggacgaa gcgaagctta aagcgaataa agaagcaaaa aaaatcatta ttcagtctat
    10861 acaacgaact gccgctgaaa cagccattga aaataccgtt tcagttttca acttaagtag
    10921 cgatgagcaa aaaggacaaa tcattggacg agaaggcaga aatatcagag cattagaagc
    10981 agcaactggt gtagaaatta ttgtagatga cacacctgag gcaattatta tttctggtta
    11041 tgatgcttac aaacgcgaaa ttgctcgttt gtcgctacaa cgcttggtca ccgatggtcg
    11101 tattcatcct gcaagaattg aagaagtcgt ggcaaaaacg gaaaaacaat tagagcaaca
    11161 aatcatggaa attggcgaac gcaccgttat cgatttagga attcatggtt tgcacccaga
    11221 gttgattcgt cttgtaggta aaatgcgatt ccgttcttct tatggtcaaa atttattaca
    11281 acactcacgt gaagttgcca acttatgcgc aatcatggcg agtgaattag gcttaaattc
    11341 taaaattgca aaacgtgcag gtttactaca tgatatcggc aaagtatctg atgaagatcc
    11401 tgaattatca cacgcattat taggcgcaaa actttgtgag aaatttggcg aatcaccagt
    11461 gattgtcaat gctgttggtg cacaccacga cgaaatggaa atgatgtatt tggtatcgcc
    11521 aattatacaa gcatgtgatg ctatctctgg tgcgcgacca ggtgcgcgtc gcgagattat
    11581 ggaaagctac atgaaacgta tcgacgaatt agaaaagctg gccgtatcct atcaaggtgt
    11641 gcaaaaggcg tatgccattc aagcaggaag agagttgcgt gtattagtcg aagcagaaaa
    11701 agtagatgac aaacaagtgg aagaaatttc atttaaaatt gcacagcaaa tccaagatga
    11761 aatgacgtat ccaggacaaa tcaaagtaac cgtaattcgc gaatctagaa gcgtttctgt
    11821 tgctaaataa attaagaaaa cacaatcata ataaagcaag aatctaatac attcttgctt
    11881 tttctttttc atcttcttcg aagactacat tcgaataatc ttgcaataca ttataaacgg
    11941 tatcgcgctc taaaggacgg cgaccaactg cttttattaa atgcaccaac tcgcgtgtgc
    12001 tcatcgcagg atgctgttct tcgctgcctg ccatagcata aattttcgtc gtatcgtcaa
    12061 tagtaccatc aacatcatca acaccaaagt tcaatgacaa ttgcgttgta ttgcgaccta
    12121 tcattgccca atacgattta atatgttcga aattatctaa ataaattcta ctgatggcat
    12181 aattgcgcaa atcctctgtt attgtcgctt ctggcagatg cttcatttca ttatgctgat
    12241 ttctaaactt caatggaata aacgcattaa aaccattcgt tctgtcttgc aactgacgaa
    12301 gtcgctccat gtgatcaata cgatgttcat atttttcgat atggccatac aacatggttg
    12361 cattacttgg aatacctaat tgatgcgcta cttcgtgtat cgccaaccac tcttccgcgc
    12421 tacattttcc gccggcaatt tcgtcgcgaa tagcagcatc aaaaatttct gcgccaccgc
    12481 caggcattga gtcaagacct gcagctttca ttgcgcgcat gccatcttca tagctcattt
    12541 ttgctttttt caaaatataa tgatattcta caggcgtcaa tgctttaata tgcaaatcag
    12601 gtcgatgtgc cttaatggct ttaaacaaat cgatataaaa attaaaatcc tgttttggaa
    12661 ttacgccgcc agtaatatgc acttctgtta caggctgtcc atcatatttt ttgacaatat
    12721 ttataatgtc atcgatagaa tattcccaac cttgctcgcg attttttatc aatcgagaat
    12781 aagaacaaaa cttacaagta taaacgcaaa tatttgttgg ctcgacatgg aaattacggt
    12841 tgaaataagt attgttgccg tgttttttat tgcgaatata attcgccaac atacctacaa
    12901 aacccaactc tcctttttca tacaacaaaa ccccttcatc aaaagtgata cgttcgtttt
    12961 gcaaaacttt ctctgcaatc tgttttaaca catggtcaat ttgtaaattt tgcagaatgg
    13021 tatatatgga atcagcgctc gtattcatat cagaataatt gattttctat cctacaaaaa
    13081 tacggaattg cctattaata gtgaattaag ttataactaa ttcgagtaac tttgcagtgt
    13141 gaagaatgaa aagtccatat tgtatttgct cacctttatt tggttttgtg ccattgtcga
    13201 ttttctattg atgttgccat taggcactga aatgatggaa atttacaata ttcaacccaa
    13261 agaatatagt tacttgattt ttgcattttc cttgagtggt ggtttatctt cttttttagc
    13321 ttctttttat atagatagct atgacagaaa aaccatatta ttatgtgcat ttttcttttt
    13381 tgccattggc agtattctgt gttcgtttgc acacacttac gaacttatgt tattttccag
    13441 attttttact ggcatttttg gcggcttgtt aggtggcgtg tctacagcat acgtaagtga
    13501 tatggtgcct tacgaacgca gaggaaatgc gatgggaatt ttaaatcttg gatttggttt
    13561 ggcatctatt ttgggcattc cgtttgctat ttttatatgt gaacatttag atgttttttg
    13621 gccatttaga attatcggca tattgtcttt agttgtgctg gtgccagctt attttttctt
    13681 accttctatg cgtaatcata tacctgaaga caacacaact atacacgata catttaaagc
    13741 gatattttca ttagcaatat atccgtttac tttttatttt tttccacgct tttcacaaca
    13801 actcaaacaa aatatacaaa aaacaaaaga agtttttgat gtattaaaaa atagaaactt
    13861 acaaagagca ttatcatttg cattttttct agtattaggt catttcatgt ttatctcttt
    13921 tatcaatcca tatttagtag gaaatttagg ttttaaacta gaagacacaa aatggatgta
    13981 tatcattggt ggtttgtcgg tttccttaac atcgccacgc atcggaaaat tcatcgatac
    14041 acttggaaaa gtaaaatcat ttagaatatt aatcattctt tcgttctttc ctattctcat
    14101 tattgcgcat ctgcaacaag cttcaatatt catggcttta ttaatttgtg catttatgtt
    14161 tatatttaat agtggtcgta tgattgctgc aatgacatta attacaggcg caccgacaga
    14221 agaacaacgc ggaaaatttt tagtcattcg ttcgtctctg attgaaatga gtgaaggtgc
    14281 ttctgcatta atcggtggca tgattttgac aaaagatgag cttaccggac atcttaataa
    14341 ttatcactac atcgcttatt tagctgtatt aattggttta ctgtgtattt atttagcaag
    14401 taaaattgag ataaacgaag agtagttttt ctaaaaaatg agtatattta ttatgattgc
    14461 tgaaaatgtt atattcaaca aaaatatact gtctgcttct cttaatgaat gtatgcatct
    14521 caaacgcaca aaacataagt ggtaggtgga ttggcaaacg cacgagtggt gcttcgctca
    14581 ctttagatat acagcaatgc aaagattcca tttttggcac agcttacatt atttttcctg
    14641 aagaaatagg cagcgccaat gtagcattta gtggcaatat acaaaatggc attctagaat
    14701 atgaaacgac aaaaatatta gaacaaaatg tggattctgc ggtattactc tgtctggtaa
    14761 ctggcagaga tatcttgaaa ataaaaaagc ggaaacaaat actagaaggc gcttgtattt
    14821 caattgataa aaaagaacca tgctttaact tgtcttgtat agaacgctat gaaaaaaaaa
    14881 tggaattcac ttttcataaa aatctatttt tgaataaaaa agtcttgatt gtcgatacaa
    14941 tatgggcaag ccaagacagc ttagatatca aagtttggga caatttaaaa gaagatggcg
    15001 atatcgtatc tatttatttg aacgagcata atatacttga aaaatacacg ctcaaacatc
    15061 aacaaaaaac aataaaacta gcctgtacaa aaacatataa caacattgtt ttttatgcgc
    15121 acaacgtagg cagcgaagca ccaaacacgg caagtatagg aatctaccag aatggaaaat
    15181 tattaaaaga aatcgtatta aaatctgatg ctgaaaaaag tgaaagcttt gtgatacttc
    15241 gcagtaatta agttgtaacg atatcacaca cagcatcgac ccaattatct tccgaattta
    15301 aactttctac caaagtaata tgttcaccac catattgttc aaacaactct aaatattcta
    15361 ctcgaatttc atataaggtt tctaagcagt cggccacaaa tgctggcgaa aatatgagta
    15421 atttcttttt gcctgcctta cccaattgtg gtataacatc gacagtatat ggttctaacc
    15481 aaatctcttt tcctaatcgc gattgaaatg tgaccgtata tttatctttc gagagtccaa
//
LOCUS       DAOQQV010000059        15444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1224_length_15444_cov_8.052375, whole genome shotgun sequence.
ACCESSION   DAOQQV010000059 DAOQQV010000000
VERSION     DAOQQV010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15444
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1224_length_15444_cov_8.052375"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="PLL59_07425"
     CDS             complement(<1..1527)
                     /locus_tag="PLL59_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HMY23675.1"
                     /translation="MQFLLATLNSKYIHTNLAIRIFYQLNKNKENIHWKEFTINEDAD
                     GIVSYCTAYDVIAFSCYIWNITQTLAVAKKIKTKNPSIKILLGGPEVSYEYQNVIALP
                     YIDFIIQGEGEIPLQAFFQSYPNVEEVPALIYKTDDNKIIENKVAAQIPLDALAEIMP
                     YSDDDKKSLKTKVLYIETSRGCPYKCEFCLASLDNKVRYFPNASIKRNLLYLMQHGRV
                     IKFLDRTFNLKKDFTIDIFQFILDNHTGNNIFQFEITADIIHPDIVQFIHDYVPKGLF
                     RFEIGIQTVNQKANLAVSRKQNFEKTKSIIKTLENKIELHLDLIVGLALDYYDDIKYS
                     FEEVFKLFAPELQLGFLKFLKGTPVRDKHQQHGFVFDPNPPYQIIESNYLSKTELKKI
                     VLLEHALEIYWNKGRAINTLKYIALHYSIFDFLQGLGTYFSCVSNLHRYTLKDVYEII
                     YQFANSNYTDDIIVKEMIAIDYYLHHKVRPQQLIIQDLPKNEKLKILQENKLNTQNFS
                     Y"
     gene            complement(1585..2688)
                     /locus_tag="PLL59_07430"
     CDS             complement(1585..2688)
                     /locus_tag="PLL59_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23676.1"
                     /translation="MRILIIVLLSSIIFIQCNNSEAQKNKLSQEENNTSNNDGNSSYE
                     KKNNWQKLEMFELKNNKGIVDALIPLPATWKLNTEGATMIGPNGIRVTDFPGKSFMIN
                     YDPSLQYAYSQSPQREMPGIDRLIEEDIIPEVSKKGWQYIRHYEIPEISKMDKWYSDQ
                     LYKAVPSKTDVRVYGTEWKKDNGEKAFIILHLNVSNTQQMQTWYYFYSMLVADADAFE
                     LAKKQLVFGLSNMRYNLEPIMAYNREEAARVGQSWETFNRKMKANQAAFEASQIAHVN
                     KSNAINDAIMSGWKAQNAASDKNQEQFIDNIYENQKVRNTETGQTYKVQQGYNQYWMN
                     NDGEYIGTKSNTYNPNLDDNMNDKKWEELKEVR"
     gene            complement(2799..3860)
                     /locus_tag="PLL59_07435"
     CDS             complement(2799..3860)
                     /locus_tag="PLL59_07435"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HMY23677.1"
                     /translation="MGFKNISDKNPVAVIGAGSFGTAVANLLAENGEVLFYSRRPEAV
                     EIINTERVNNGQSIHPNIRATCDMQELTDKCYLIFPSVSSDGFRQVMQNMAPYLRPDH
                     MMIHCTKGFDIKLEKDKTILDKDVLLKPADIYTMSKVILEETCVKRVGCMSGPNLARE
                     IANLQPAATVIASKFDEVIREGENAIRSKRLQVYSNHDIYSVELAGVMKNSMALAAGA
                     LGGLGYGQNAMAFLITRGLGEIIRLTSAMGANKQAFLGLAGIGDLVATCTSPMSRNYT
                     VGARLAKGETLEQIIATSSEVAEGIKTVSISKKLADSTGIKVPIIQTIYKVLFEELNV
                     EEALGFLMEYRWGFDADYM"
     gene            complement(3838..5505)
                     /locus_tag="PLL59_07440"
     CDS             complement(3838..5505)
                     /locus_tag="PLL59_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMY23678.1"
                     /translation="MLKSVIPRIKDWPINLIAQKREKILQECISEAVLEFNKSNPTLS
                     QLRNQLQKTLYLENIRIKNESWEVDPEDEREFWGKIKKRLLQADPNLVDEDVSKEQHQ
                     EMLQDIMSRYANEIIGHFNPNIFWFARQLLVRFFARLFNSHFGGIRGMLHPQEKLKDK
                     LIVAGPVEKLRNLSLKGTVILVPTHFSNLDSAIIGFGMDYIGMPAFQYGAGLNLFNSK
                     FFSFFMGNLGAYKLDRRKKNPIYLETLKSYSKTNVLAGAHTIFFPGGTRSRAGNIETE
                     LKLGLLGTVMEAQRIHFEKNPANLAPKIFIVPLTLSYNFVLEASSLIEDQLKRSGKEQ
                     YLVKEIPTATGKGIWKFFWETFSKSTDITLSLSEPMDVFGNALDDDGNSIDKFGKKIE
                     IKNYFVSKGQLKADDQREMIYTQMLGDKIIEGFFKNNVVYSSHVVTFVAFELINKQLD
                     FPDLFTLMRSSEEDREINFDTFKTAVKRVIDELYMLNNKGQIKLSPFISSKELDEIIE
                     LGITNVCIYHTNLPIMRLKNGNISSEDLKLLYYYHNRLLGYGLQKYI"
     gene            5608..6039
                     /locus_tag="PLL59_07445"
     CDS             5608..6039
                     /locus_tag="PLL59_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMY23679.1"
                     /translation="MRFDIKYIEENDALEKLIAKGEGEQLDFKQSISNAKKIAKTLVA
                     FANTTGGKLLIGVNDSGLIVGCEIEEEMYMIDLAITQYCQPKIAVEFSVYEFDEMLNV
                     LVVDVKNNSIKQAYFSLDDNDEWQYYIRHRDKTIHTHVDDK"
     gene            complement(6051..7340)
                     /locus_tag="PLL59_07450"
     CDS             complement(6051..7340)
                     /locus_tag="PLL59_07450"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMY23680.1"
                     /translation="MDLKIPSTGKKRVVIIGGGFGGIEVAKKLKNLDVQIVLLDKHNY
                     HCFQPLLYQVATGGLEPGSIAYPLRKFMQDIPNGLFRMAEVRHVDTAIQKIHTDIGDL
                     HYDHLIIATGSTTNFFKFPNEVSDKMMQMKDIPQALNLRSFILENFEEALLTYDEEKK
                     EELINISIVGGGPTGVELAGALGEMRKNILPKDYPEIDFRRMQIHLFESSDRILSAMH
                     IDNSSKALKYVKDFGVNVWLNTMVTDYNGDELTIADGKKIKCGTVIWTAGVKGNPIGG
                     LADAAMAGNRILVDEYCQVLHHNNIYAIGDVAAMINEQFPKGHPMVAPVAIQQGDLVA
                     KNIIASIKNKEIKSFKYFDKGAMATVGRNKAVMEAKGMRMGGFIAWLAWLFVHVMTLI
                     GFRNKITVTLGWLYNYITYDRTLRLIIRPFKNNNEKQ"
     gene            7500..8258
                     /locus_tag="PLL59_07455"
     CDS             7500..8258
                     /locus_tag="PLL59_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HMY23681.1"
                     /translation="MSHIHLVKKDNIGIIQMDRDKANPMNHEFVAALRIAFKNLLEDD
                     RIDGVILNGKENFFSAGLDLPELYNYDEQQFDNFWRNFMELIYDMIAFDKPLVASITG
                     HSPAGGCVLAICCDYRVMAEGKYVIGLNEIPVGLVLPRALFEIYSFWLGTRTAYQYGL
                     EGKLISPAQAKEIGLVDEVVAPTAVLEAAEAQLKRYLLFDKKSWRITKKQMKQNLLKS
                     ITNTSDFEMNTFVQQWWSEPVRTGIKQFVDKLKK"
     gene            8267..9208
                     /locus_tag="PLL59_07460"
     CDS             8267..9208
                     /locus_tag="PLL59_07460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY23682.1"
                     /translation="MQDKFIQFENRLIKVYRHLSKIAKKQEVSCYRLYNVDLPEFPFI
                     IDIYKQTVYVAEYKSKHKLTDDEYADWLQTSLQIIGNVLQVSQDNIYLKLRERQKGDQ
                     QYNKLQQEKKELIVEENGLSFIVNLSDYLDTGLFLDHRLTRQKVKEISAQKNVLNLFA
                     YTGSFSVYAAAGNANKIVTVDMSKTYLNWARRNLAYNKLYDDNKHFFIQDDTLHFLKN
                     ETHNSYDLIILDPPTFSNSKRMDGTLDIQRDHVDIINRCLLLLKENGILIFSTNYRSF
                     KLDASSLATTKIKDITKQTIPFDFEGKLQRACYWIEK"
     gene            complement(9209..9724)
                     /locus_tag="PLL59_07465"
     CDS             complement(9209..9724)
                     /locus_tag="PLL59_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23683.1"
                     /translation="MKQVFYLLIGFLCITNMYAQHDVAAIEYNNKIVNEQTKIGEAIL
                     SFSSNPNAFSLAKLKEQAESSMSIIKKMKAYKDDKQLLSAAKDMFKFYISISKKDYKE
                     LLYLVENKEKYESNELTRKLNTLVKNISAKEIPLDNAFQSAQKKFAEKYGFTLQKNDL
                     SDKLKDATKEN"
     gene            complement(9726..10196)
                     /locus_tag="PLL59_07470"
     CDS             complement(9726..10196)
                     /locus_tag="PLL59_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23684.1"
                     /translation="MRKLFGILLLLIVVNISKAQNYNVEAYKIHQSIVEAQQNIGKEV
                     YEFNATATALNLKILKFEINRSIAFIDSLKINKDDSTYYQAAKQLFSLYKDLTEHEYA
                     DLLKIVEDPDLEYKDFKAKKIKIYDAMKAKSAAVYPIFNAAQESYCKKYKLKIE"
     gene            complement(10261..11082)
                     /locus_tag="PLL59_07475"
     CDS             complement(10261..11082)
                     /locus_tag="PLL59_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23685.1"
                     /translation="MDEDKKLSYCNTKYPVILVSGLGFKDQNRIINYWNTIPDFLKQY
                     GCEVYTAQQDAFISIPDNAIKLKYRILDILSQTKHEKINIIGHSKGGLEARYLASRLG
                     MENQISSITTLGSPHRGTHLADIVLGRLPLPKYATARLVNLYATLLGDKRPDSLRAVV
                     QVTTESMKQFNDEVPDAANVYYQSYASHVNKEYPNMLWKTMAYILREYEGRNDGMVGI
                     ESSQWGNYRGLIQSETASSVSHGDMVGMTQFFGNPAFKANLFFKQLIHELKEMQH"
     gene            11384..12220
                     /locus_tag="PLL59_07480"
     CDS             11384..12220
                     /locus_tag="PLL59_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HMY23686.1"
                     /translation="MKPKIAIQGFEASFHEIVALQYFGNEIETVECATFPKLFSVMAN
                     GEAEYAVCAIENSLAGSILPNYAHLRNSNLKIIGEVYHRIEMNLMALPNQTIQDINEI
                     HSHPMALLQCRKFIENHQSIQIVESNDTALSAKEIKDNHIKHRAAIASKRAATVFNLE
                     IIAADIHDNKRNFTRFLILADAATHIAHASKINKSSISFRAHHTPGSLAKVLTTIGEY
                     GINLTKIQSLPVIGEEWQYYMYADLEFEDIEQYKKMIEEIKPVTKELKILGEYAQGDK
                     IV"
     gene            12241..13413
                     /locus_tag="PLL59_07485"
     CDS             12241..13413
                     /locus_tag="PLL59_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMY23687.1"
                     /translation="MIIQAANKLQHINEYYFSMKLKQIAQMRAEGKPIINLGIGNPDQ
                     APSTATIDALITAAANVTNHGYQSYIGIPELRTALSNWYKKTYEVALQPTTEVLPLLG
                     SKEGISYISHAFLNPGDKVLVPNPGYPTYTSATYLAGAEPVSYNLDASNNWAPDFTSI
                     PESVLQEAKIIWINYPNMPTGAAGNQEIFSNIIALAKKYNILVVNDNPYSLVLNEGQP
                     VSILQTAQAKDVCLELNSLSKSHNLAGARIGMIAGAKDYIDTILKVKSNVDSGMYLPV
                     QHAAIAALSNSDEWHLARNNEYKKRRTLAYAIMDELECVYDTNQIGMFLWAKIPDRYE
                     NVEVLTEHILQDANVFITPGFIFGSNGDRYIRISLCSPEKDLQEALIRIKNTKSSV"
     gene            13426..14268
                     /locus_tag="PLL59_07490"
     CDS             13426..14268
                     /locus_tag="PLL59_07490"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006307.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HMY23688.1"
                     /translation="MKVAIVGIGLIGGSTAIDLRAAKFCTEILGVGRSQQSNETALKL
                     GLVDKIVTKEEALQYADLIILTVPVNILIDELKYYLDNMQSHQVITDMGSTKGIIIDA
                     IQNHPNRKQYVASHPMAGTENSGPAAAIPHLFKDKVCIICDKEKSDQRAVALIEQLYQ
                     TLNMRIKYMDAHQHDMHAAYISHISHISSFVLAATVLEKERDEEAILEMAGGGFESTV
                     RLAKSSPDMWAPIFQQNKLYLLEVMDTYIEKMYHFRNLINKNKNEELRQFMQNANEIR
                     KILK"
     gene            14360..>15444
                     /locus_tag="PLL59_07495"
     CDS             14360..>15444
                     /locus_tag="PLL59_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HMY23689.1"
                     /translation="MSNLTIVPLKDWGFNFEKPVIISGPCSCETEEQMMETAQRLANK
                     GIHVLRAGIWKPRTRPGMFEGIGKEGLSWLVNAGKEIGLPTTVEVANAQHVEESLAAG
                     VDILWIGARTTVNPFSVQEVADALRGVDIPILVKNPINPDLQLWLGSMERLNAVGITK
                     LGAIHRGFSSYEKSKYRNKPMWELPVEMRRLYPNMPIIVDPSHICGSRELIPSVCQKA
                     LDLDFDGLMIESHRDPDNAWSDAAQQVTPERLGEIISSLVVRSSKLDNYAENQLEALR
                     AQIDRIDNYMLEIMGERMDIARKIGEFKRDNGITILQTNRWDEIIHDRVKKAAKKDLT
                     EDFVKDMMEAIHQESIRHQTKIMNNILA"
BASE COUNT         4994 a         2551 c         2573 g         5326 t
ORIGIN      
        1 gtagctgaag ttttgagtat ttagcttatt ttcttgtaaa atttttaatt tttcattttt
       61 tggtaagtct tgaataatca attgctgcgg tcttacttta tggtgtaaat aataatcaat
      121 ggcaatcatt tcttttacaa tgatatcatc agtatagttg gaatttgcga attggtaaat
      181 gatttcatac acatctttta aagtatagcg atgaagatta ctgacgcatg aaaaataagt
      241 gcctaagcct tgtaaaaaat caaaaatgct ataatgtaat gcaatatatt ttagtgtgtt
      301 tattgctctt cctttattcc aataaatttc taaagcatgt tcgagtagta caattttttt
      361 taactcggtt ttgctcaaat aattactctc aataatttga taaggtggat ttggatcaaa
      421 cacaaaacca tgttgttgat gcttatcacg aactggtgtt ccttttaaaa atttcaagaa
      481 acctaattgt aattcaggag cgaaaagctt aaaaacttct tcaaacgaat attttatatc
      541 gtcgtaataa tctaacgcca aaccaacaat taaatcaagg tgtaattcga ttttattttc
      601 gagtgtttta atgatggatt ttgttttttc aaaattctgt tttctgctga cggcaagatt
      661 tgctttttga tttactgttt gaatgccaat ctcaaatcgg aacaaacctt tcggcacata
      721 atcatgaata aattgaacaa tatctggatg aatgatatct gctgttattt cgaattgaaa
      781 gatattattt ccagtgtgat tgtctaaaat aaattggaaa atatctatcg tgaaatcttt
      841 ttttagattg aaagtgcgat ctaaaaattt gattacacga ccatgttgca tcaagtaaag
      901 taaatttctt ttaatgcttg catttggaaa ataacgcact ttattatcta ggctagctaa
      961 acaaaattcg catttgtaag gacaaccacg agaggtttct atgtataaaa cttttgtttt
     1021 taaactcttt ttatcatcat cgctatatgg cataatttct gccaatgcgt ctaatggaat
     1081 ttgcgcggct actttgtttt caattatctt attgtcgtct gttttataga ttaaagcagg
     1141 cacttcttcg acatttggat aagattgaaa aaatgcttgc agtggaattt ctccttctcc
     1201 ttgaataatg aaatcaatgt atggtaatgc tatcacattt tgatattcat agctcacttc
     1261 tggtccacca agcaaaattt ttatacttgg attttttgtt ttaatttttt tggcaactgc
     1321 aagtgtttgt gtgatattcc aaatgtaaca gctaaatgca ataacatcat aagcagtaca
     1381 ataggataca ataccatcag catcttcatt gatggtaaat tctttccaat gtatattttc
     1441 tttattttta tttaattgat aaaaaatacg aatggctaag ttggtatgaa tatattttga
     1501 attaagtgta gccagtaaaa attgcatacc acaaagataa acttttaaga tagaacctga
     1561 aatcgctaaa tttgaagctt attgttatct aacttctttt aattcttccc attttttgtc
     1621 gttcatattg tcatctaaat ttggattata ggtgttagat tttgtgccaa tatattcacc
     1681 atcattattc atccaatatt gattataacc ttgctgtact ttataagttt gtccagtttc
     1741 tgtatttcgc actttttggt tttcataaat attatcaata aactgttctt ggtttttatc
     1801 gcttgctgca ttttgtgctt tccaaccaga cataatcgca tcgtttatcg catttgattt
     1861 atttacatga gcaatttggc tggcctcaaa tgcggcttgg tttgctttca tttttctatt
     1921 aaatgtttcc cagctttgac caacgcgcgc tgcttcttct ctgttatatg ccattatggg
     1981 ttcaagatta taacgcatat tggagaggcc aaatactaat tgttttttcg ccaactcaaa
     2041 tgcatctgca tcagcgacta gcatagaata aaaataatac catgtttgca tttgttgtgt
     2101 attgctgaca tttaagtgta aaatgatgaa ggctttctcg ccattatctt ttttccattc
     2161 agtaccatat acacgtacat cagtttttga tggaactgct ttgtataatt gatcgctata
     2221 ccatttatcc atcttactaa tttctggaat ttcataatgc cttatatact gccatccttt
     2281 ttttgaaact tctggtatga tgtcttcttc tattaatcta tcgatgcctg gcatttctct
     2341 ttgtggcgat tgagaatagg catattgcaa acttggatca taatttatca taaatgactt
     2401 tcccggaaaa tctgtaactc taataccatt tggaccaatc attgttgcgc cttccgtatt
     2461 tagtttccac gttgctggta atggaattaa agcatctact attcctttgt tgttttttaa
     2521 ttcaaacatt tctagcttct gccaattgtt tttcttttcg tatgaagaat tgccatcatt
     2581 atttgatgta ttattttctt cttgagaaag cttgtttttc tgtgcttcgc tattattaca
     2641 ttgtataaat ataatcgacg ataatagcac aataatgagt atgcgcatag ttttggtttt
     2701 ttaataaagt ataaattaaa attacatttt taatacctaa aaagcaaatt ggaataagta
     2761 taaaagtgat attaataagt agtattaagt gcgaatcgtc acatataatc tgcatcaaat
     2821 ccccaacggt attccattaa aaatcctaat gcttcttcga catttaattc ttcgaataat
     2881 actttataaa tagtttgaat aataggtact tttatgccag tgctatcggc taatttcttt
     2941 gaaatactta ctgtttttat gccttctgcc acttcagatg aagtagcaat aatttgttct
     3001 aatgtttcgc ctttagcgag tcgtgcgcct acggtatagt ttctactcat aggcgaagta
     3061 catgttgcca ctaaatcgcc gataccagcc aatccgagaa aagcttgttt gttggcaccc
     3121 attgcactgg taagtctaat aatttcacct aatccgcgag taatgagaaa agccatagca
     3181 ttttgtccat atcctaaacc acctagagca cctgctgcta atgccattga gtttttcata
     3241 acgccagcta attccacaga atagatatcg tgattgctat atacttgtaa tcttttggaa
     3301 cgaatggcat tttcgccttc gcgaattact tcatcaaatt tactggcaat cacggttgct
     3361 gctggttgca gattggcgat ttctcttgct aaatttggac cactcataca gccaacacgc
     3421 tttacacatg tttcttccaa aattacttta ctcatggtgt aaatgtcggc tggttttaac
     3481 aagacatctt tatcgagtat ggttttatct ttttctaatt ttatgtcaaa accttttgta
     3541 caatgaatca tcatgtgatc tggccgaaga taaggcgcca tgttttgcat tacttgccta
     3601 aatccgtcgg aggaaacaga aggaaaaatg agataacatt tatctgtaag ctcttgcata
     3661 tcgcatgtag ctctaatgtt tggatgaata gattgcccgt tatttacacg ttccgtattg
     3721 ataatttcta ctgcttctgg tcggcgtgaa tagaataata cttcgccatt ttctgccaat
     3781 agattggcaa ctgccgtacc aaagctgcct gcaccaatta ctgctactgg atttttatca
     3841 gatatatttt tgaagcccat agccaagtag tctgttgtga tagtaataca ataattttaa
     3901 atcttcgctg gaaatatttc cattcttcaa acgcataatc ggaagatttg tatgatagat
     3961 acaaacattg gtaatgccca actcaattat ttcatctaat tctttggaag aaataaatgg
     4021 agatagtttg atttgtcctt tattattaag catatataac tcatcgatta ctctttttac
     4081 tgccgtttta aatgtgtcaa aattgatttc acgatcttcc tctgagctac gcattaaggt
     4141 aaataaatca gggaaatcga gctgtttatt gattaactca aatgcaacaa aagtgacaac
     4201 atgagaagag tacacaacat tgtttttaaa gaatccttca atgattttat cgccgagcat
     4261 ctgtgtgtaa atcatttcgc gttggtcgtc agcttttaat tgtcctttgc taacaaagta
     4321 atttttgatt tctatttttt tgccaaattt atcgatgcta ttgccatcgt cgtcaagcgc
     4381 atttccaaaa acgtccatag gctcgcttaa tgacaaggtg atgtctgtag atttggaaaa
     4441 tgtttcccaa aagaatttcc aaatgccttt gcctgttgct gtaggaattt ctttcaccaa
     4501 atattgctct tttccagagc gtttgagttg gtcttcgatg agtgatgatg cctctaaaac
     4561 aaagttataa ctgagtgtta gcggtacaat aaaaatttta ggagctaagt ttgctgggtt
     4621 tttttcgaag tgaatacgct gtgcttccat cacggttcct aataatccga gcttcaattc
     4681 tgtttctata ttgccagctc tagagcgcgt gccaccaggg aaaaatatag tgtgcgcacc
     4741 agctaaaaca ttagttttgg aataagattt taaggtttct aaataaatag gatttttctt
     4801 gcgtctgtcc aatttataag cacctaaatt gcccataaaa aaactgaaaa atttagagtt
     4861 aaataagttt aggcctgcac catattgaaa tgctggcatg ccaatataat ccatgccgaa
     4921 gccaataata gctgaatcta aattgctgaa atgtgtaggt actaaaataa cagtaccttt
     4981 taaagataaa tttcttaatt tttctactgg accagcaacg atgagtttgt cttttaattt
     5041 ttcttgtgga tgcaacatgc ctctgatgcc tccaaaatga gaattaaata agcgtgcaaa
     5101 aaatcgaact aataattgtc tggcaaacca aaaaatattt ggattaaaat gaccaataat
     5161 ctcattggca tatcgcgaca tgatatcttg cagcatttct tgatgttgct ctttagatac
     5221 atcttcgtca acaaggttag gatctgcttg taataatcgt ttttttattt tcccccaaaa
     5281 ttctctttca tcttcaggat cgacttccca cgattcattt ttaatgcgaa tattctcaag
     5341 gtaaagtgtt ttttgaagtt gatttctaag ttgtgataaa gtagggttgg acttattaaa
     5401 ttctaataca gcttctgaga tacattcttg caatatcttt tctctttttt gcgctatcaa
     5461 attgataggc cagtctttta ttctcggaat gacagatttt aacatgcgta tggttcaact
     5521 aatacggtaa aaatagggtt taaattataa aaatgaaaga atattttcaa gtgcgaactt
     5581 gttttttatt ttattaattt taatgtaatg cgatttgata taaaatatat tgaagaaaat
     5641 gatgccttag aaaagctaat tgcaaaagga gaaggagaac aattagattt taaacaatcc
     5701 atttctaatg caaaaaagat agctaaaaca cttgttgcat ttgctaatac tactggtgga
     5761 aaattattga ttggcgttaa tgatagtggt cttattgtag gctgtgaaat agaagaagaa
     5821 atgtatatga tagaccttgc aattactcaa tattgccaac caaaaattgc tgttgaattt
     5881 tctgtatatg aatttgatga aatgctcaat gtactcgttg tagatgtgaa aaataatagt
     5941 atcaaacaag catatttttc attagatgat aatgatgaat ggcagtatta tattcgccat
     6001 cgtgataaaa ccattcatac acatgttgat gataaatgac aataagtacc ttattgtttt
     6061 tcgttattat ttttaaatgg cctaataatc aaacgcaaag ttctatcata ggtaatgtag
     6121 ttatatagcc agccaagtgt aaccgtaatt ttgtttctaa atccaatcaa tgtcattaca
     6181 tgcacaaaca gccaagccag ccaagcaata aatccgccca ttcgcatgcc ttttgcttcc
     6241 ataacagctt tgtttctgcc aacagtagcc atagcacctt tgtcaaaata tttgaatgat
     6301 tttatttctt tatttttaat agaagcgata atattttttg ccactaaatc gccttgctgt
     6361 atggcaacag gagcaaccat tggatgacct tttggaaatt gctcatttat cattgctgca
     6421 acgtcgccaa ttgcgtaaat attgttatgg tgtaatactt gacaatattc atctactaaa
     6481 attctgttgc cagccattgc tgcatctgct aagcctccaa tcggatttcc ttttacgcct
     6541 gctgtccaaa tgacggtgcc acatttgatt tttttaccat ctgctattgt caattcatcg
     6601 ccattataat cagtcaccat cgtgtttagc cacacattta cgccaaaatc ttttacatat
     6661 tttaatgctt ttgaagaatt atcaatatgc atagcgctga gtattctgtc gcttgattca
     6721 aacaagtgta tttgcattct tcggaagtcg atttcaggat aatcttttgg taaaatattt
     6781 tttcgcattt cgcctaaagc accagctaat tcaacacctg ttgggccacc acccacaatt
     6841 gatatattta tcaattcttc ttttttttct tcatcatacg tcaacaatgc ttcttcaaaa
     6901 ttttccaaaa taaagcttct taagtttaat gcttgtggaa tatctttcat ttgcatcatc
     6961 ttgtcagata cttcatttgg aaacttaaaa aaatttgttg tacttccagt agcgatgatt
     7021 aaatggtcgt aatgtaagtc gccaatatct gtatgaattt tttgtatcgc tgtatcaaca
     7081 tgacgtactt ctgccattcg aaaaagacca tttggaatgt cttgcataaa ctttcgcaat
     7141 ggataggcaa tgctgcctgg ctctaatcca cctgttgcta cttgatataa taaaggttga
     7201 aaacagtgat aattatgttt gtctaacaat acaatttgaa catctaagtt ttttaatttt
     7261 tttgctactt cgatgccacc aaaaccaccg ccaatgatga cgactctttt tttaccagtt
     7321 gaaggaattt ttaagtccac gtttttattt ttagcttttt tataaagttt gtatcagtaa
     7381 atatattcac taattacttt acaaaagtac aagtgtaagt ttgttttaag attaaatgtt
     7441 ttatacacta ctgttatttc tatatattta cattttattt tgtttttaaa acatacaata
     7501 tgtcacacat acaccttgtc aaaaaagata acatcggcat tatacaaatg gatagagata
     7561 aagccaaccc aatgaatcat gagtttgttg ccgctttacg aattgcattt aaaaatttat
     7621 tagaagatga taggatagat ggtgttattt tgaacggtaa agaaaatttc ttttctgctg
     7681 gcttagattt accagaatta tataattatg acgaacaaca attcgataat ttttggcgaa
     7741 attttatgga attaatttat gacatgatag catttgataa accgcttgtc gcttctatta
     7801 caggtcattc acctgctggt ggttgtgtgc tggcaatttg ctgtgattat agagttatgg
     7861 cggaaggaaa atatgtgatt ggtttgaatg aaattccggt tggcttggta ttgcctcgtg
     7921 cattatttga aatttatagt ttctggttgg gcactcgaac tgcctatcaa tatggcttag
     7981 aaggaaaatt aatttcgcca gcacaagcaa aagaaatcgg attggtagat gaagtagtag
     8041 cgccaacagc agtccttgaa gcagcagaag cacagttgaa acgctatttg ctattcgata
     8101 aaaaaagttg gcgtataacg aaaaaacaaa tgaaacaaaa tctgcttaaa tctattacaa
     8161 atacatcaga ctttgaaatg aatacatttg tacaacaatg gtggagcgaa cctgttcgca
     8221 caggaataaa acagtttgtt gataaattga aaaaataata aatacagtgc aggataaatt
     8281 tatacaattt gaaaacagat tgatcaaagt atatcgccat ctttctaaga tagcaaaaaa
     8341 acaagaggta agttgctatc gattgtataa tgttgatttg cctgaatttc cttttattat
     8401 tgatatttac aagcaaacgg tatatgtcgc tgaatacaaa agtaaacata aactaactga
     8461 tgatgaatat gctgattggt tgcaaacttc gttacaaatt ataggcaatg tgttgcaagt
     8521 ttcacaagat aatatttacc taaaactacg cgaacgtcaa aaaggcgatc aacaatacaa
     8581 caaactacag caagaaaaaa aagaattaat agtagaagaa aatggattga gttttattgt
     8641 taatctttct gattatttag acactggttt attcttagac catagattaa cgcgtcaaaa
     8701 agtgaaagaa atttctgcac aaaaaaatgt gcttaatctt tttgcataca caggaagttt
     8761 ctctgtatat gctgctgccg gaaatgcaaa taagattgtc acggttgata tgtcaaaaac
     8821 ttatttgaat tgggctcgcc gcaatcttgc atataataaa ttatatgatg ataacaagca
     8881 tttttttatt caagatgata cgttgcattt cttgaaaaat gaaacacaca attcatatga
     8941 tttaatcata ctcgatccgc ctacattttc caatagcaaa agaatggacg gcactttaga
     9001 tattcaacgc gatcatgtag atattattaa tcgctgtttg ttgctcttaa aagaaaatgg
     9061 aattttgatt tttagtacta attatagaag tttcaaactt gatgcttcat cattggcaac
     9121 tacaaaaata aaagatatca ccaagcaaac aattcctttt gattttgaag gcaagctaca
     9181 acgtgcttgt tattggattg aaaaataatt aattttcttt cgtggcatct tttagcttat
     9241 cgctcaaatc atttttctga agcgtaaatc catatttttc tgcaaacttt ttttgtgctg
     9301 actgaaaagc attatccaat gggatttctt ttgcagaaat attttttact aatgtgttta
     9361 atttcctcgt aagctcgttg ctttcgtatt tctctttatt ttctaccaag taaagcaatt
     9421 ctttataatc ttttttagaa atactgatgt agaacttgaa catatctttt gcggcactta
     9481 aaagttgctt atcatcttta taagctttca ttttttttat aatactcata ctgctttcag
     9541 cttgttcctt caactttgca agtgaaaatg catttggatt tgaagaaaaa gagagaatag
     9601 cttcgcctat tttagtttgt tcatttacaa ttttattatt gtattctatt gcggcaacat
     9661 cgtgttgcgc atacatgttt gtaatgcaga gaaatccgat tagtaaataa aatacttgtt
     9721 tcatcttatt caatttttag tttatatttt ttgcaatagc tttcttgtgc cgcattaaaa
     9781 ataggatata cggcagcaga tttagctttc atagcatcat agatttttat ttttttagct
     9841 ttaaaatctt tgtattccaa atctggatct tctactattt ttaataaatc tgcatactca
     9901 tgttctgtta aatctttata caaactgaat aattgttttg ctgcttgata ataagtagaa
     9961 tcatctttat tgatttttaa actatcaata aatgcaatac ttctattgat ttcaaatttt
    10021 aatattttaa gattgagtgc tgttgcagtt gcattaaact catacacttc tttgcctata
    10081 ttttgttgtg cttctacaat tgattgatgt attttataag cttctacatt ataattttga
    10141 gctttgctaa tgtttacaac aattaaaagt aataatattc caaagagttt tcgcatgtat
    10201 aaaaatttat taaagatatc aataaaaatg ccaaaaatac aaatctacaa cttagcttct
    10261 ttaatgttgc atttctttca gctcatgaat caattgttta aaaaataaat tagccttaaa
    10321 cgcaggattg ccaaagaatt gtgtcatgcc aaccatgtcg ccatggctca cagacgaggc
    10381 cgtttctgac tgtattaaac ctcgataatt tccccattgg ctcgattcaa tgccaaccat
    10441 gccatcattt ctgccttcat attctcgaag tatataggcc atcgttttcc atagcatatt
    10501 cggatattct ttattgacat gactcgcata actctgataa tatacgttag cggcatcagg
    10561 cacttcgtcg ttaaactgtt tcatgctttc cgtcgtcact tgcaccactg ctcttaagct
    10621 gtcaggccgt ttgtcgccta agagtgttgc atacaaattt actaatcttg ctgttgcata
    10681 ttttggtaaa ggcagtctgc ctaaaacgat atctgctaaa tgcgtgcctc tatgtggcga
    10741 ccctaaagtg gtgatgcttg atatttgatt ttccatacct aaacgactag ctagatatct
    10801 tgcttccaag ccacctttcg agtgaccgat aatatttatt ttttcgtgct ttgtttgtga
    10861 taaaatatct aatattctat actttagttt tatcgcatta tcaggaatag aaataaatgc
    10921 gtcttgttgt gcagtatata cttcacagcc atattgcttg agaaaatctg gaattgtatt
    10981 ccaataatta ataattctat tttgatcttt aaatccaaga ccacttacca aaatcactgg
    11041 atattttgta ttacaatagg atagcttttt gtcttcgtcc ataacttatt accttaataa
    11101 aaacaaactt ttcttctgct tgttgttacc gatgtcgtga atttagcaac atttttattg
    11161 attttttttc atcttaacca tgaattaata ttgatatagt tgcattttaa acaaaatctt
    11221 ttttaatttg tgttatgaat aaattttatt tctacggtta ttatttcttt cgcaactaag
    11281 cgagacggat gatactgtag tagtaataag tataataagt ctcgagtaat atcgagactt
    11341 tttttttgaa tatactcgat tgaaaatgaa aatttaattg caaatgaaac caaaaatagc
    11401 catacaagga tttgaagctt ctttccacga aatagtagca ttgcaatatt ttggaaatga
    11461 aatagaaacg gttgaatgtg caacatttcc caaattattt agtgtaatgg ctaatggcga
    11521 agctgagtat gctgtttgtg ctatcgaaaa ctccttagct ggatctatat tgcccaatta
    11581 tgcgcacttg cgcaattcta atttgaaaat aattggagaa gtatatcatc gaattgaaat
    11641 gaatttaatg gctttgccca atcagacgat tcaagatatt aatgaaattc attctcatcc
    11701 aatggcatta ttacaatgca gaaaatttat tgaaaaccat caaagcatac aaatcgttga
    11761 atccaatgat acagcattaa gtgcaaaaga aattaaagac aatcacatca aacatcgcgc
    11821 tgccattgcc agcaagcgtg ctgccacagt ttttaactta gaaataattg ctgctgatat
    11881 tcatgacaac aaaagaaatt ttactcgttt tttgatttta gcagatgctg caacgcatat
    11941 tgctcatgct tccaaaatca ataagtcgtc tatttcattc agagcacatc atacaccagg
    12001 cagcttagca aaagtattaa ctaccattgg cgaatatggc attaacctaa caaaaattca
    12061 atcattgcca gtaatcggtg aagaatggca atattatatg tatgctgatt tagaatttga
    12121 agatattgaa cagtataaaa aaatgattga ggaaattaaa ccagttacta aagaattaaa
    12181 aatattaggc gaatacgcac aaggtgacaa aatcgtttag catccgaaat aaaatacaat
    12241 atgattatcc aagctgcaaa taaattacaa cacatcaatg aatactactt cagtatgaaa
    12301 ttgaaacaaa ttgcacaaat gcgtgctgaa ggaaaaccaa taataaatct aggtattggc
    12361 aatcctgatc aagcaccttc tactgcaact attgatgcat tgataacagc agcggccaat
    12421 gttacaaacc acggctacca aagttatata ggcattccag agttaagaac agccttaagc
    12481 aattggtaca aaaaaacgta tgaggttgct ttgcagccaa caacagaagt attgccattg
    12541 ttaggttcaa aagaaggaat atcttatatt tcacatgcat ttttaaatcc aggcgataaa
    12601 gtattagtgc ccaatcctgg ctatccaact tatacgtcag ccacttattt agcaggagcg
    12661 gagccagtct cttataactt agatgcatcg aataattggg cacctgattt tacgtctata
    12721 cctgagtccg tattgcagga ggcaaagata atttggataa actatcccaa tatgccaact
    12781 ggtgcagccg gtaatcagga gatattttct aatataattg cattagcaaa aaagtataat
    12841 attttagtgg ttaatgacaa tccatatagt ttagtgttga atgaaggtca acctgtttct
    12901 atattgcaaa cagcacaagc taaagacgtg tgtttggaat taaattcact aagcaaatca
    12961 cataatttag caggtgcacg aataggcatg attgctggtg ctaaagatta tatcgacaca
    13021 attttaaaag tgaaaagcaa tgtagatagt ggcatgtatt tgccagttca acatgcagca
    13081 atagccgctt taagtaatag cgatgaatgg catttggcac gcaataatga atacaagaaa
    13141 cgcagaactt tagcgtatgc aataatggac gaattggaat gtgtgtatga taccaatcag
    13201 ataggtatgt ttctttgggc aaaaatacct gatcgatatg aaaatgttga agtattaaca
    13261 gaacacattt tacaggatgc aaatgtcttt attacaccag gttttatttt tggtagtaat
    13321 ggtgatcgat acattcgtat ttctttatgt agtcctgaaa aagatttaca agaagcatta
    13381 ataagaataa aaaataccaa gtcatcagtc taattctaaa tcaacatgaa agttgcaatt
    13441 gtaggtatag gtttaatagg aggctcaaca gcaatagatt tgcgtgcagc aaagttttgt
    13501 acggaaatct taggcgttgg tagaagtcag caaagcaatg aaacagcatt aaaattaggc
    13561 ttagtagata aaattgtaac aaaagaagaa gccttacaat atgcagattt aattatacta
    13621 acagtacctg taaacatact aattgatgag ctaaaatatt acttggataa tatgcaatcg
    13681 catcaagtaa ttacagatat gggttctaca aaaggaatta ttatagatgc tatacaaaat
    13741 catccaaaca gaaaacaata cgtcgcttcg catccgatgg ctggtactga aaatagtgga
    13801 cctgcagcag caattcctca tttatttaaa gataaagttt gcattatttg tgataaagaa
    13861 aaaagcgatc aacgtgcagt cgctttgata gaacaacttt atcaaacgct taacatgcgc
    13921 attaaataca tggacgcaca tcagcacgat atgcatgcgg catatatttc tcatatttca
    13981 cacattagtt catttgtatt ggctgctact gtgttagaaa aagaaagaga tgaagaggct
    14041 attttagaaa tggctggtgg tggttttgaa agcacggttc gattagcgaa aagttcgcct
    14101 gatatgtggg cgcctatttt tcaacaaaac aaattgtatt tattagaagt aatggataca
    14161 tacattgaga agatgtacca ttttagaaat ttaattaata aaaataaaaa tgaagaatta
    14221 agacagttta tgcaaaatgc caatgaaatc agaaagatat tgaaataaaa ctttttgaaa
    14281 tactttgcat aattaaaact tattctattg gtaaaacaaa ggtttttaat aaaatataaa
    14341 taacaaataa atttacatta tgagcaattt aactatcgta cccttaaaag attggggttt
    14401 taattttgag aaaccagtca tcatttctgg tccgtgttcc tgcgaaacgg aagaacaaat
    14461 gatggaaacg gcacaacgtt tggcaaataa aggcatacat gtacttcgtg caggcatttg
    14521 gaaaccacgt acacgaccag gaatgttcga aggtattggc aaagaaggct tgtcatggct
    14581 tgtaaatgct ggtaaagaaa taggcttgcc aacaacagtt gaagtagcaa atgcacaaca
    14641 cgtagaagag tccttggctg caggtgtcga tatactttgg ataggagcgc gcacaacggt
    14701 taatccattt tctgtgcaag aagtagcaga tgcattacgt ggtgtagata ttcctatttt
    14761 agtaaaaaat cctattaatc ctgatttaca attgtggtta ggcagtatgg aacgtttaaa
    14821 tgctgttggc atcactaaat taggcgcaat tcatcgtggt ttttcctcgt acgaaaaatc
    14881 taaatacaga aataagccaa tgtgggaatt gccggtagaa atgcgtcgtt tgtatccaaa
    14941 tatgcctatc attgttgacc cttctcatat ctgtggttct cgcgaattaa ttccttctgt
    15001 ttgccaaaaa gcgcttgact tagactttga tggcttgatg atagaatctc accgcgatcc
    15061 agataatgct tggagtgatg ctgcgcagca agtaacgcct gagcgtttag gtgaaatcat
    15121 ttcttcattg gttgttcgaa gtagcaaatt agataattat gcggaaaatc aattagaagc
    15181 attacgcgct caaattgaca gaatcgacaa ctatatgttg gagattatgg gcgaacgtat
    15241 ggatatcgca cgtaagattg gtgagttcaa aagagataat ggaattacca tcttacaaac
    15301 caatcgttgg gacgaaatta ttcatgatag agtaaaaaaa gcagcgaaaa aagaccttac
    15361 agaagatttt gttaaggata tgatggaggc aattcaccaa gaatctatcc gtcatcaaac
    15421 aaaaattatg aacaatattt tagc
//
LOCUS       DAOQQV010000060        15289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1252_length_15289_cov_7.977091, whole genome shotgun sequence.
ACCESSION   DAOQQV010000060 DAOQQV010000000
VERSION     DAOQQV010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15289
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1252_length_15289_cov_7.977091"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLL59_07500"
     CDS             <1..872
                     /locus_tag="PLL59_07500"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMY23690.1"
                     /translation="DSITDAVHPIYRNHVNYAVFITMLLPFIFLARTWYSPKSFPYKL
                     LNFSVLFFLLAIYLSYTRGAWLAVIAMLVYFFILKWNATKQLLLIAGIAVISFTVYIL
                     NDNNYLKYSPNYDTTIYHDELGDHLSSTFEMEDMSTVERFYRWIAAVHLFKEHPLLGV
                     GPNNFVSHYKPYTVTAYETYISDNEEKSTVHNYFLLLLTEQGLPALILFICLLIVVLL
                     TAQKLYNQVSTTQRKYIVAITLCIIAFLLNNSLSDLVEANKVGSLFYICLALLLHFSV
                     NNISTEEDINKLT"
     gene            complement(865..2097)
                     /locus_tag="PLL59_07505"
     CDS             complement(865..2097)
                     /locus_tag="PLL59_07505"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMY23691.1"
                     /translation="MNLTEAEINADNLLALLKVKNKQLHALLDATKAINHNVSSEGLY
                     KLYKNTLSEQLQVKEVALFMYDQTWKRVVWEQSVPNFYIPIQLQFDNYKNASELSEEE
                     QKIFGNFKFIIPVYHKQQPLALALLGNIPEDVIHQKEEMLEFAQTFTSIITVAVENKR
                     LFKKEVEKKQYDKELDLASKVQGMLIPKKLPKNTMYEFAGMYLPFNGIGGDYYDVIHI
                     SKDEFVFCIGDISGKGVAAALVMANLQAYLNATLGLGFTREKFIEKLNQKIYSVTNGE
                     NFITLFIAKYNIITRELEYLNAGHIPPILLHHNKTMLLDSGCALLGAFEELPSIEFGN
                     ITLEPNTTIVCFTDGITELEDDAGVQFGTERMLEFSSNNYKLGPDIFNKILYDYVCKF
                     KGNVLFNDDISVLTAKFM"
     gene            complement(2249..2677)
                     /gene="bcp"
                     /locus_tag="PLL59_07510"
     CDS             complement(2249..2677)
                     /gene="bcp"
                     /locus_tag="PLL59_07510"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HMY23692.1"
                     /translation="MLQEGTKAPKIKGVDFKGKKTILYFYPKDDTPGCTANACDFRDN
                     YASLTKQGFSIIGVSPQDEKSHDKFKAKYDLPFELIPDTDKTIAQAYEVWGKKKFMGR
                     EYDGILRTTFVINEKGIIEKIITKVDTKNATKQLLKVLSE"
     gene            complement(2694..4370)
                     /locus_tag="PLL59_07515"
     CDS             complement(2694..4370)
                     /locus_tag="PLL59_07515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMY23693.1"
                     /translation="MKKTCFLFIFFVCFILFSNISYSNGHRDIGFIKPMQLPFNLAGS
                     FAEPRKDHFHSGIDIKTNGKEGEPVFAIGDGYISRIRVSPYGYGKAIYITHNNGYTSV
                     YGHLSAFYNEIGEYITQQHYAKQKSELDLYFDATKLPIKQGDTIAFSGNTGGSTAPHL
                     HFEIRDSKTEHALNPLDFYPNEFYIDSISPQLNAIKITQQNGQLQETYNLIKHANYWT
                     TNMPIYCCSQAPLALSLEGFDKQDTSSSKNGIIKMELFKDDSLHFGYHIQSVNFDDTR
                     MCNAFIDYHEMMNNNGYFYNCYQMPNNTLPIYVAGMNGLLQIEKHKTSHVLINCYDYN
                     DNKTTIHLTLIGDTTNNTTACANTMNTIENKTFSSFNISFPDKAFYKKEDIIQVQKNA
                     SKKCLSDTFMIAGKYSIIPLQQAAKIRFNSAIKKNRSKIIIVRQDFFGKENALKTKSD
                     KHNFYADTKELGSFYLKYDTTKPIIELIHLPQQDVNKNIKIKIIDVLSRIDSYNGYID
                     NNWVNFYYDAKNDEITYQLDEHCTKGEHLLKITVTDKVGNKQTLVQKFNY"
     gene            complement(4458..5276)
                     /locus_tag="PLL59_07520"
     CDS             complement(4458..5276)
                     /locus_tag="PLL59_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23694.1"
                     /translation="MFRNSHISIIIAILLMVIVGACNKPVSNENKIKQSDFKIEDNVN
                     GRVPPPNNIIESTKFFYNEDGTLSSATVHDDTTSNAHLLKEVNIIYGADKIIVDTYLD
                     TIGNVRYYIYFNEKKQVTSLMLNDSVGLIFSYLDDRMASIRLAPTANEYLGFIYDEND
                     NLLQYSFRFNNQIVHRYLLQYNNDEIKKEFDSRFLSKYIKFIYIGGLDLIDKLGLNYG
                     KSSQNKLVKRTDIDLSNDEVIDTYIFNYSYDNKARINKRNIQFSTDTLYYDFKY"
     gene            5390..6835
                     /locus_tag="PLL59_07525"
     CDS             5390..6835
                     /locus_tag="PLL59_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23695.1"
                     /translation="MYDSKLIALISTFSIEDKRKLRKWIKSDFVNKNTDIISFFQFID
                     TRSTLTAKTLAKEKAHLYMYPNLPYQDLRIRHLIWQTTEIVEQFIIYNKVTQQPALSG
                     QLLAQYYTDNELYKFAEQSTDESLTLIQQSKLQNASYHLYQYHIQALSFHIQTRKNRS
                     EVFNLQKVIDHATIFSVIETLKYACVVQSMQKITEANIDIHLLDTTLKLIHENSLFLQ
                     EISIRVYYNTYLMIKDESEQAYEQLIDDIKSHSSFFSISDLRDLYMFAISFCVKKSNQ
                     NIARFTRETFELYIYTIQNGILLENKVISRFSFTNVVTLGIKLKEFTATEEFIKQYAK
                     LISKEFQKNTIDFNNAKLLFAKGNYQKALQILLTNEFKDILWELNSKYIVLKIFFETK
                     DIIRFPIFLKAFKNFIKRKSNIGYHKNYFVNVSIALTQLKELQQKPAMAQHFKFDAST
                     PDIDWFNKALASMQGSKKQNKKADTKPTLKK"
     gene            complement(6848..7561)
                     /locus_tag="PLL59_07530"
     CDS             complement(6848..7561)
                     /locus_tag="PLL59_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMY23696.1"
                     /translation="MSLEDFKKKKPFKQRSWTQVNASNSWSMFRIMSEFVDAYDKMDK
                     IGPCISIFGSARTKPDNPYYQMATAVAERLSEEGYGVITGGGPGIMEAGNLGAQNKDG
                     KSVGLNIELPFEQGHNPYIDFDKLITHHYFFVRKVVFVKYAQGFVYLPGGFGTFDELF
                     EVMTLVQTKKIDRVPIVLMGKAFWQGMLDWMRTEVLNNQHNISENDFNLFLVTDDVEE
                     AIAYINTFYTEHDLAPNFS"
     gene            complement(7643..9106)
                     /locus_tag="PLL59_07535"
     CDS             complement(7643..9106)
                     /locus_tag="PLL59_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMY23697.1"
                     /translation="MSSTLILLCILFYFCLLIGIAYFTGKNADSDSYFIGNKKSVWYI
                     VAFGLIGDSLSGVTYISVPGSVNAAQFNYFQMVLGYVIGYWTISYVLLPLYYKMQLTS
                     IYTYLLNRIGVSTQRTGAFFFILSRIIGAGARLYLASIVLQRFVFDGFGIPFPVTVAI
                     IIVLMLVYTYKGGIKTLVWTDMLQSSFLLLGVIFSIYFISTQLDLSLGGMISSIKESD
                     YSQVFFWDWKAKNYFWKQFIGGAFIAICMTGLDQNMMQKNLSCKSLGEAQKNINAFSI
                     IVVIVNLLFLGLGALLYIYANSKQIIIPERSDALFPFLAFNHLGTFAGLVFVIGLTAA
                     TFNSADSVLTTLTTSFYIDFLGKETDNTQEHTKLRMSIHAGFAVVLFITVLLFGAFSD
                     QSMIFLVMDIAGITYGPLLGLFAFGIFTKKMPIDKLVPLVCIVSAACCTFLYFSSKYK
                     AAWLNGYAFGQELLLINGFITFIGLWLISQRATKLSV"
     gene            9195..9812
                     /gene="recR"
                     /locus_tag="PLL59_07540"
     CDS             9195..9812
                     /gene="recR"
                     /locus_tag="PLL59_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HMY23698.1"
                     /translation="MQFPSKLIEDAVHAFATLPGIGKKTALRLVLHLLQNETANTKQL
                     ADAILKMRMEIKFCQTCHNVSDADICSICSNGYRDKSVVCVVENFRDIIAIENTRHFN
                     GLYHVLEGLISPMDGIGPEQLNIQSLASRVQTGEIQEIIMALNPTIDGDTTIFYLSKM
                     LSKYEVKITSIARGIAFGGELEYVDEITLARSIQSRLPYENYLSK"
     gene            10004..11641
                     /locus_tag="PLL59_07545"
     CDS             10004..11641
                     /locus_tag="PLL59_07545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY23699.1"
                     /translation="MKLSIVIVNYNVRYFLEQTLLSVYQSNVQFEYEIFVVDNHSSDD
                     SVKMVQQKFPLVKLLANTENVGFSKANNQAIKQAIGEYILLLNPDTIIQEDTLQCCID
                     KMESDATIGGLGVKMYDGAGNFLPESKRGFPSPMAAFAKMSGMAKMFPKSKLFGQYHL
                     SYLDNNQTHEIDVLSGACMLLRKSVLDKIGHLDEDYFMYGEDIDLSYRIRKAGFKNVY
                     LANTSIIHFKGESTKKGSLNYIKVFYNAMLIFTNKHFTGSSAQVLKFILHIAIYLRAI
                     LAVIKKIFSNILAPIIDIVLMSSSLYALHLFWENVVKEEAHVIFPSTFFYVNIPIYVL
                     IWCASMWLFGVYAKDAKWRRLLLSVIFGTLLITIVYAFFPNYLRTSRGIIVCGMLLNI
                     FLLSAYRCLYYWQNGSLNTYIHNEKKYIIIGSEIEATTIATQIKQAMPKRKYLGFVAL
                     QDGQLHTKKLGTIENINEILKAFKPNEILFSTADLSTQFIIETMARIEEPIAFRLVTK
                     NNTIISSTSKNKSGEIYSFDINLTSKQSWIDKLRNWF"
     gene            12072..12593
                     /locus_tag="PLL59_07550"
     CDS             12072..12593
                     /locus_tag="PLL59_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23700.1"
                     /translation="MACQKVVVEKEHPFVTYTIRAGEQYADYFEYEELTSITEMKFVA
                     IFDSSAIYTSAIPTNQEDVNKLYGFADNNMHHHINSARIGWRWFNNELQLFAYVYNNA
                     VRSFVFIKSVNLLQAIHCAIKINGNKYLFTVDGTSIEMPRTASVADGYKLFPYFGGDE
                     FAPHEIRIRIKDM"
     gene            complement(12679..14163)
                     /gene="guaB"
                     /locus_tag="PLL59_07555"
     CDS             complement(12679..14163)
                     /gene="guaB"
                     /locus_tag="PLL59_07555"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HMY23701.1"
                     /translation="METLSHFSSNKFAGEGLTFDDVLLLPAYSQILPRETNLRTQVTR
                     NISINIPILSAAMDTVTEANLAIALAREGGIGVLHKNMSIDAQAAEVRKVKRSESGLI
                     TDPITLKPTSKVADAFALMSENRIGGIPITNESGILVGILTNRDLRFCKDKNMLIQDL
                     MTKERLITAPEKTTLKAAEEILHQYRIEKLPIVDKNNKLVGLITYKDILKVKNYPNST
                     KDNSGRLRVAAAVGVTADMMNRMEALVAVGVDFICIDTAHGHSQGVLSSIKKAKETFK
                     ELDIVGGNVATGAGAKALAEAGVDAVKVGVGPGSICTTRVVAGVGVPQLSAIYDASLA
                     LKNTGVPIIGDGGIRYTGDMVKALAAGANTIMAGSLFAGSEESPGKTVIFEGRKFKTY
                     RGMGSLEAMQEGSKDRYFQDVEDDIKKLVPEGIVGRVPYKGTLSEIVYQFVGGLRAGM
                     GYCGAADIPSLQATAQFVKITTAGVNESHPHDVTITNEAPNYSR"
     gene            complement(14328..14867)
                     /locus_tag="PLL59_07560"
     CDS             complement(14328..14867)
                     /locus_tag="PLL59_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23702.1"
                     /translation="MKKLFVVALLYTFFTSVNAQENRTKIEEAILTKNSLIKQEIFSV
                     DNIPAFRIEAVKITNLEKFNISTGLRIVHRVQEGKVYKNTYNYIDQSEIDGLIVSLQY
                     MKTMVKNKTLPSNYTEIKFATMSGFQITLAMVVNAQNNLDWNFATQTNTSNEKTLTIL
                     PIDNIEKLQKVLEQAKGKL"
     gene            complement(14921..>15289)
                     /locus_tag="PLL59_07565"
     CDS             complement(14921..>15289)
                     /locus_tag="PLL59_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMY23703.1"
                     /translation="NIIGALSMLVLEKKKDIATLTALGIQKNSIVRIFLFEGILLSCA
                     GALIGMLLAFLLCLLQQTVGLIPMPGTSFLVNYYPVKMMASDFLIVGAVVFCISCIAA
                     YLPAKKAARAVENEYLSYLS"
BASE COUNT         4935 a         2610 c         2609 g         5135 t
ORIGIN      
        1 tcgattccat cacagatgca gtgcatccaa tttatcgcaa tcatgtcaat tatgctgtat
       61 ttattacgat gttactgcct tttatctttt tggcgcgcac ttggtattca ccaaaatcgt
      121 ttccatataa attgctgaat ttttctgtct tatttttttt gttggccatt tatttgtcgt
      181 acacacgtgg tgcatggctg gctgttattg ctatgctggt ttattttttt atattaaaat
      241 ggaatgcaac aaaacagctt ttgctcattg cgggtattgc tgttatatca tttaccgtgt
      301 atattcttaa tgacaataat tatttgaaat attcaccgaa ttacgacact acgatttatc
      361 atgatgaatt aggtgatcac ctatcttcta catttgaaat ggaagacatg agcacagtgg
      421 agcgttttta tcgctggatt gcagccgtac atttgtttaa agaacatcca ctacttggcg
      481 tcggtccaaa taattttgtg agccattata agccttacac ggtcacagcc tatgaaacat
      541 acatcagcga taatgaagag aaatcaacgg tacacaatta ttttttgtta ttgcttacag
      601 agcaaggttt gcctgctttg attttgttta tatgcttatt gatagtggta ctgctcacag
      661 cacaaaaatt gtataatcaa gtaagtacga cacagcgtaa atatattgta gccattactt
      721 tgtgcatcat tgcattttta ctcaataact cattaagcga tttagtagaa gcgaataaag
      781 tgggatcatt gttttatatc tgtttggctt tgttgctgca ttttagtgtc aacaatattt
      841 cgacagaaga agatataaat aagcttacat aaatttggct gttaacacac tgatatcgtc
      901 gttaaacaac acattgcctt taaatttaca gacgtaatcg tataatattt tattgaaaat
      961 atcagggccc agtttatagt tgttactact aaattccagc attctttcgg tgccaaattg
     1021 aacgccagca tcatcttcta attctgtgat gccatcagta aaacaaacta tcgtggtgtt
     1081 gggttcaagc gtgatattgc caaattcaat agatggcaac tcttcgaaag cacctagtaa
     1141 ggcacagcca ctgtcgagta acattgtttt attgtggtgt aataaaatgg gcggaatgtg
     1201 acccgcgttt aaatattcta attcgcgcgt gatgatgttg tatttggcaa taaataaagt
     1261 aataaaattt tcgccattgg ttacagaata tattttctga ttgagttttt caataaattt
     1321 ttctcgtgta aatcctaagc ctaatgtagc atttaagtaa gcttgtaggt tggccatcac
     1381 caaagcggca gccacgcctt tgcccgaaat atcgccaatg caaaacacaa attcatcttt
     1441 actgatgtga atgacatcgt agtaatcgcc gccaatacca ttgaaaggaa gatacatacc
     1501 tgcaaattca tacattgtgt ttttgggaag cttcttcggg atcagcatgc cctgtacttt
     1561 tgatgccaaa tccaactctt tgtcgtattg ttttttctct acttcctttt tgaaaagcct
     1621 tttattttct acagctaccg tgataatgct ggtaaatgtt tgtgcaaatt ccaacatttc
     1681 ttctttctgg tgaattacat cttctggaat attgcctaat aatgctaatg ctaatggttg
     1741 ctgcttgtgg taaacaggaa taatgaattt gaaattgcca aatatttttt gctcttcttc
     1801 gctaagctcg ctcgcatttt tgtaattgtc aaattgcagc tggataggaa tgtagaaatt
     1861 aggaacagac tgttcccaca caacacgttt ccaggtttgg tcatacataa aaagcgcaac
     1921 ctcttttact tgtagttgtt ctgataaagt gtttttgtat aatttataca aaccttctga
     1981 agaaacatta tgattgattg ccttagtcgc atcgagtaaa gcatgcaatt gcttgttttt
     2041 tactttaagc aatgccaata aattatctgc gttgatttct gcttctgtca ggttcatcaa
     2101 aataaatcta cgcaaaaaaa aatcaagaaa gatgtaacag aaatgattaa tttttcacct
     2161 gcataaaaaa taacgaattt tagtgatgca tttgaatgtt attacacaag aaaatcaaat
     2221 aaaattcaat ctttcaacaa aaatttattt actcagacag cacttttagt agttgctttg
     2281 tagcattttt ggtatctact tttgtgatta ttttttcaat aattcctttc tcgttaatca
     2341 caaaagtagt acgtaaaatt ccatcatatt cacgacccat aaacttcttc tttccccaaa
     2401 cttcataagc ctgtgcaatg gttttatctg tatcaggaat aagctcgaaa ggtaaatcgt
     2461 attttgcttt aaacttatca tgtgattttt catcttgtgg tgaaacgcca ataatgctaa
     2521 aaccttgctt cgtcagtgaa gcataattgt ctctaaaatc acaagcattg gcagtgcatc
     2581 ctggtgtatc gtcttttgga taaaaatata aaattgtttt tttgccttta aaatctacac
     2641 cttttatttt tggtgctttt gttccttctt gtaacatgct tatttttttg agtttaataa
     2701 ttaaattttt gaactaaagt ctgcttattt ccaactttat ctgtaacagt tatttttaaa
     2761 agatgttcac cttttgtgca atgttcatcg agctggtaag tgatttcgtc gttcttggca
     2821 tcgtaataaa aattgaccca attattatcg atgtatccat tatacgagtc aattcttgaa
     2881 agcacatcaa ttatttttat tttgatattt ttgttgacgt cttgttgtgg taaatgtata
     2941 agctcaatga ttggttttgt tgtgtcatat tttaaataga atgaacctaa ttcctttgtg
     3001 tctgcataaa aattatgctt gtctgatttt gtttttaatg cattttcttt tccaaaaaaa
     3061 tcttgtctta caataataat cttacttcta ttttttttga tagctgaatt gaacctgatt
     3121 tttgcagcct gttgtaatgg tataattgaa tacttgccag ctatcataaa tgtatcagac
     3181 aaacattttt ttgatgcatt tttttgcacc tgtatgatgt cttctttttt gtaaaatgct
     3241 ttatctggaa atgaaatatt aaaactcgaa aatgttttat tttctatggt gttcatagtg
     3301 tttgcgcaag ctgtagtatt gttagtagtg tcgccaatca gtgttaaatg gatagtagtt
     3361 ttgttgtcat tgtaatcata acaatttata agtacatgag acgttttatg cttttctatt
     3421 tgtagcaatc cattcattcc agcaacataa ataggcagcg tgttgttggg catttgatag
     3481 caattgtaga aatagccatt attattcatc atttcatgat aatcgataaa tgcattgcac
     3541 attcgagtgt cgtcaaagtt tactgattga atatggtagc cgaaatgcaa agagtcatct
     3601 ttgaataatt ccatttttat tatgccattc ttgctactcg aagtatcttg tttgtcaaat
     3661 ccttctaatg acaaagctag tggagcttgg ctacaacagt aaattggcat gtttgtcgtc
     3721 caataatttg catgttttat aagattatat gtttcctgca attgcccatt ttgttgcgtt
     3781 atttttatag catttaactg cggcgaaatg ctgtctatat aaaattcatt aggataaaaa
     3841 tctaaaggat tgagcgcatg ctctgttttg ctatctcgta tttcgaagtg caagtgtggt
     3901 gcagtgctgc cgccagtatt acctgaaaat gcaatggtat cgccttgttt tataggtagc
     3961 tttgtagcat caaagtataa gtctaattct gatttttgtt tggcgtaatg ttgctgagta
     4021 atgtattctc caatttcgtt ataaaacgcg ctgagatgtc cataaacact agtatatcca
     4081 ttgttatgcg tgatgtaaat agcttttccg tatccgtatg gcgacactct aattctggaa
     4141 atatagccat cgccgatagc aaaaacaggc tcaccttctt tgccattggt ttttatgtct
     4201 atgccagaat gaaaatgatc tttgcgcggt tccgcaaagg agccagccaa attaaatggc
     4261 aattgcattg gtttgatgaa gccaatatcg cgatgcccat tggaatacga aatattgcta
     4321 aacaatataa agcaaacgaa aaagatgaat aggaaacatg tttttttcac acattaaaaa
     4381 tatcaattct aaaggaaaat aaaaagctct gaaaaatcag agcttctggt gaactaatct
     4441 aaaagattct aagaaaatta atacttaaaa tcatagtaca aagtgtctgt actaaattgg
     4501 atatttcgtt tgttgattct tgctttgttg tcatacgagt aattgaaaat ataagtatca
     4561 atgacttcgt cgttgctaag gtcaatgtct gttcgtttta ctaatttgtt ttgtgaactt
     4621 ttgccataat ttaagccgag tttatctatt aaatctaagc caccaatgta aatgaatttg
     4681 atatactttg acaagaaacg gctgtcaaac tcttttttga tttcatcgtt gttgtattgt
     4741 aataaataac gatgtacgat ttggttgtta aatctaaatg agtattgcaa taaattgtca
     4801 ttttcatcat aaatgaaacc aaggtattca tttgctgttg gtgctaatct gatactcgcc
     4861 attctgtcat ctaaatagga gaaaattaaa cctacagaat cattaagcat caaactcgta
     4921 acttgttttt tctcattgaa atatatataa tatcgaacat tgccaattgt gtctaaataa
     4981 gtgtcaacaa ttattttgtc tgcgccgtaa atgatattta cttcttttaa taaatgcgca
     5041 tttgatgtcg tgtcatcatg cacggttgca ctactcaatg ttccatcttc attatagaaa
     5101 aactttgtag attcaataat attattcggt ggtggcacac gaccatttac attgtcctca
     5161 attttgaagt cagattgctt aatcttattt tcgttggaaa caggcttgtt gcaagcgcct
     5221 acaatgacca ttaaaagtat ggcaattatg attgatatgt ggctgtttct aaacatgaga
     5281 ataagagtct ttactgtcaa tatacaaaga ttcaattatt ttttttaatt ttccggtaca
     5341 aataaatgcc tttgtcataa aatttaggca ctcaataaat aatactttta tgtatgattc
     5401 aaagttaatt gctttaatat caacgttttc tattgaagat aaacgtaaac ttcgcaagtg
     5461 gataaaatct gattttgtca ataaaaacac cgatataata tctttttttc aatttattga
     5521 tacacgttca acgctaacag caaagacttt agccaaagaa aaggcgcatt tgtacatgta
     5581 tccgaacttg ccttatcaag atttgcgcat tcgtcatctg atttggcaaa ccacggaaat
     5641 agtagaacag tttatcattt ataacaaagt aacacaacag cctgcattga gcgggcaact
     5701 tttggcgcaa tactatactg ataatgaatt atacaaattt gcagaacaat caacagatga
     5761 aagtttgact ttaatccaac agtcaaaatt gcagaatgct tcgtatcacc tatatcaata
     5821 tcatattcaa gcattgtcat tccatattca gacacggaaa aacagaagcg aagtatttaa
     5881 tttgcaaaaa gtaatagacc atgcaactat tttttctgtc atcgaaacct taaagtacgc
     5941 ctgtgtagtg cagtctatgc aaaaaataac agaagcgaat attgatatac atttactcga
     6001 cactactttg aaattgattc atgaaaattc tctttttctt caagaaattt ctattcgtgt
     6061 atattacaat acgtatctaa tgattaaaga cgaaagcgag caggcatacg aacaattgat
     6121 tgatgatatc aaatcgcaca gtagtttttt ctctatttct gatttaagag atttgtacat
     6181 gtttgcgatt agtttttgtg taaaaaaatc aaatcaaaat atagcaaggt ttacacgaga
     6241 aacatttgag ttatatatct acacaattca aaatggcatc ttgttagaaa ataaagtaat
     6301 cagccgtttt agctttacga atgtagtgac attaggtatt aaacttaaag aatttacagc
     6361 aacagaagaa tttataaaac agtatgctaa actaatatcc aaagaatttc aaaaaaatac
     6421 tattgacttt aataatgcga aactgctatt tgccaaaggc aattatcaga aagcattaca
     6481 aatattactg accaatgagt ttaaagatat tttatgggaa ttaaattcaa aatatatagt
     6541 gcttaaaatt ttctttgaga ccaaggacat cattcgtttt ccaatatttt tgaaagcatt
     6601 taaaaatttc attaaacgta aatcgaatat tggctatcat aaaaattatt ttgtcaatgt
     6661 aagtattgct ttgacacagc tgaaagaatt acaacaaaaa ccagctatgg cacagcattt
     6721 taaatttgat gccagcacac cagatatcga ttggtttaat aaagcattag caagtatgca
     6781 agggtcaaaa aaacaaaata aaaaagccga cactaagccg actttaaaaa aataaatctt
     6841 gtttttctta tgaaaagttt ggcgctaaat catgttctgt gtagaaagta ttgatatagg
     6901 caatggcttc ttcgacgtca tcagtaacaa ggaataaatt gaaatcgttt tcgctgatgt
     6961 tgtgttgatt attcaatact tctgtgcgca tccaatctaa catgccttgc cagaatgctt
     7021 ttcccattaa cacgatgggt actctatcta tttttttagt ttgcactaaa gtcattactt
     7081 caaacagctc atcgaaggtg ccaaagccac ctggtaaata cacaaatcct tgagcatact
     7141 ttacaaacac tactttgcgc acaaagaaat aatgatgtgt gatgagtttg tcaaaatcaa
     7201 tgtatggatt gtgtccttgt tcaaatggca attcgatatt taaaccaaca gatttgccat
     7261 ctttattttg tgcgcccaaa tttccggctt ccataatgcc tgggccaccg ccagtaatca
     7321 cgccatagcc ttcttcagat aagcgctccg ccacagcagt tgccatctgg taataaggat
     7381 tgtcaggctt ggtacgtgca gaaccaaata tagaaataca aggtccaatt ttatccatct
     7441 tatcatacgc atcaacaaac tcactcatga tacgaaacat gctccagctg ttcgatgcgt
     7501 ttacttgtgt ccaacttctt tgtttgaatg gtttcttctt tttaaaatct tctaatgaca
     7561 taataattaa tttaaaaatt aaaagagctt tgtgtttaca caaaaacccg acttgctaaa
     7621 ttagcgatta aatcctgatt ttttataccg ataatttagt agctcgttgg ctaatgagcc
     7681 aaagaccaat aaatgtaata aacccattga tgagcaatag ctcttgtcca aatgcataac
     7741 cgttgagcca cgctgctttg tattttgatg aaaaatataa aaatgtacaa caagcagcac
     7801 ttactataca taccaaagga acgagcttgt ctattggcat ttttttagta aatatgccga
     7861 atgcaaataa gcctaataat ggtccataag tgatgccagc aatatccatc actaagaata
     7921 tcatcgattg gtcactaaac gcaccaaaca ataaaaccgt gataaacaat accacagcaa
     7981 aaccagcgtg aatagacatt ctgagcttag tatgttcttg agtgttgtcc gtttctttgc
     8041 ctaaaaaatc gatatagaat gatgtggtta atgtagttaa tacggaatca gcgctgttga
     8101 atgttgctgc cgttaagccg atgacaaata ccaagccggc aaatgtgccc aaatggttga
     8161 atgctaaaaa tggaaataag gcgtcagaac gttctggaat gattatttgt ttgctattgg
     8221 cataaatata taataatgca cctaagccaa gaaataatag attgacaata acaacaataa
     8281 tactaaatgc attgatgttt ttttgcgctt cgccaagcga tttgcagctc aaatttttct
     8341 gcatcatatt ctggtctaaa ccagtcatgc aaatagcgat aaatgcacca ccgataaact
     8401 gtttccaaaa ataattctta gctttccaat cccagaaaaa cacctgagaa tagtcagatt
     8461 ctttgatgct acttatcatt ccgccaagag ataaatctaa ttgtgtgctg ataaaataaa
     8521 tggaaaaaat aacacctaat aaaagaaatg aagattgtag catgtccgtc catactagtg
     8581 ttttaatgcc acctttatat gtgtacacca acattaaaac tataatgata gcaactgtta
     8641 ctggaaatgg aatgccaaaa ccatcgaata caaaacgttg gagaacaata gaggctaagt
     8701 ataacctagc gccagcacca attatccttg ataagatgaa aaaaaatgca ccggttcttt
     8761 gtgtcgatac gccaattcta ttcaataaat acgtgtaaat agaggtgagt tgcattttat
     8821 aataaagcgg tagcaataca tacgaaattg tccaatagcc aatcacataa ccgagtacca
     8881 tttggaaata attgaattgt gcagcgttga cagagcctgg cactgaaata tatgttacac
     8941 ctgaaagcga atcgccaata agcccgaaag caacaatata ccaaacagat tttttgttgc
     9001 caataaaata actatcgcta tctgcatttt tgccagtgaa ataagcgatg ccgataagca
     9061 agcaaaaata aaataagata caaagtagta ttagcgttga tgacatgtaa atttttttaa
     9121 ttgaaaatga gattgaaggt aataaaaaac caagacttaa gcctacaact tacatctaat
     9181 tgctacattt gtgtatgcaa ttcccttcaa agttaatcga agatgctgtg catgcatttg
     9241 ctaccttgcc tggtattggc aagaagacag cattaaggct cgtgctacat ttattacaaa
     9301 atgaaacagc aaatacaaaa cagttggcag atgcaatctt gaaaatgcgc atggaaataa
     9361 aattttgcca aacctgtcat aatgtttcag atgcagacat ttgtagtatt tgcagtaatg
     9421 gctatcgcga taaaagtgta gtgtgtgtgg ttgaaaattt tagagatatt attgccattg
     9481 aaaacactag acattttaat ggattgtatc atgtattgga gggtctaata tcgccgatgg
     9541 acggcattgg acctgagcaa ctcaacatac aaagtctagc aagcagagtg cagactggcg
     9601 aaatccaaga aattataatg gcattaaatc caacgataga tggcgatacg accatatttt
     9661 atttgtcaaa aatgctatcg aaatatgaag taaaaattac gtctattgcc cgtggtatag
     9721 ccttcggtgg cgaattagaa tatgtcgatg aaatcacatt ggcacgctca atacaatcgc
     9781 gtttgccata tgagaactat ttaagtaaat aattttaacc acgtagaaac attgaatgtt
     9841 aatgaattca acattagata ttaaaataac tcagaaatat ttagatgagt taatttataa
     9901 aattgttggt gctgctaatg gaattcatat gaatgaaatt ttcagcgaac tgcaaaaaaa
     9961 ataaatctat gttctatgtt gttaaaatag catgtaaaaa gtaatgaaat taagcatagt
    10021 catagtcaat tataatgtgc gctatttttt agagcaaaca ctcttgtctg tgtatcaatc
    10081 gaatgttcaa tttgagtatg aaatttttgt tgtcgataat cattcttctg acgattctgt
    10141 gaagatggtg caacagaaat ttccattggt gaagttgttg gcaaacacgg aaaatgtagg
    10201 tttttcgaaa gccaataatc aggcgataaa acaagctatt ggcgagtata ttttactatt
    10261 aaatccagat accatcatac aagaagatac attgcaatgt tgcatagata agatggaaag
    10321 cgatgcgaca attggtggtt taggcgtgaa aatgtatgat ggagccggaa attttctgcc
    10381 agaatcgaaa cgaggttttc cgtcgccgat ggcggcattt gccaagatga gtggcatggc
    10441 aaaaatgttt ccaaaatcaa aattatttgg tcaataccat ctgagctatt tagacaataa
    10501 tcagacacat gaaattgatg tgttgagtgg cgcatgtatg ttattgcgca aaagtgtgtt
    10561 ggataaaatt ggccacttag atgaagatta ttttatgtat ggcgaagata ttgatttgtc
    10621 ctatcgcatt agaaaagctg gttttaaaaa tgtatatctt gccaacactt ctattattca
    10681 ttttaaaggc gaaagcacaa aaaaaggtag cttaaactac attaaagtat tttataatgc
    10741 tatgcttatt ttcaccaata agcacttcac cggtagtagc gcacaagtat taaaatttat
    10801 actgcacatt gccatttatt tacgagcgat attggctgtt ataaaaaaaa tttttagtaa
    10861 catattggct cccatcattg atattgtatt gatgtcatct agcttgtatg cattacattt
    10921 attttgggaa aatgttgtta aagaagaagc gcacgtcatt tttccatcta catttttcta
    10981 tgtcaatatt ccgatatatg tgttgatttg gtgtgcttcg atgtggcttt ttggtgtgta
    11041 tgcaaaagat gcgaagtggc ggcgcctatt gctgagtgtc atttttggga cgctgttgat
    11101 aaccattgtt tatgcgtttt ttcctaatta tttacgcaca tcgcgtggca ttattgtttg
    11161 tggcatgttg ctaaatatct ttcttttatc agcctaccgc tgtttgtatt attggcaaaa
    11221 tggcagtttg aatacatata ttcataacga aaaaaaatac atcattattg gtagtgaaat
    11281 agaagcaact acaattgcaa cacaaattaa acaggctatg cctaaaagaa aatatctagg
    11341 atttgttgct ttgcaagatg gccaactaca cacaaaaaaa ttaggcacta ttgaaaatat
    11401 aaatgaaata ttaaaagcat tcaagcctaa tgaaatatta ttttccacag cagatttatc
    11461 tacacaattt atcatagaaa caatggcgag aatagaggag ccaattgcat ttcggctggt
    11521 cacaaaaaat aatacaatca taagcagtac ttcaaaaaat aaaagtggag aaatttattc
    11581 ttttgatatc aatcttacaa gtaaacaatc ttggatagac aagttgagaa attggttttg
    11641 atttttataa agaaaaattc acaatatgac tccatgtgcc atgtttgtgt accagctgcc
    11701 atggtttgtg tcctcacaaa ccatcatatt aaaacgccct cttaaatcct tttaaaaaat
    11761 caaagtcttt aattgttttg taatgtggtt cgtaaagaca ataatcaagg ctaatagaaa
    11821 agtctgccat tttttattaa cttgtattgt acccagctgc catggtttgt gtcctcacaa
    11881 accatcataa ttaaaacgct tctcttaaat ccttttaaaa aatcaaagtc tttaattgct
    11941 ttgtaatgtg gttcgtaaag acaataatca aggctaatag aaaagtctgc cattttttat
    12001 taacttgtat tgtactacat gaaaagtata tgaagtatta ttatcgtatc ttgttttttt
    12061 tcgtatgcat catggcatgt cagaaagtag tagtggaaaa agaacatcca tttgttacct
    12121 ataccataag agcaggcgaa caatatgctg attattttga gtatgaagaa ctgacttcga
    12181 ttactgaaat gaagtttgtc gccatctttg acagctcagc aatttataca tcagcgattc
    12241 cgactaatca agaagatgtc aataaactat atggttttgc tgataacaat atgcaccatc
    12301 atatcaatag tgctagaatc ggctggcgct ggtttaataa tgaattgcaa ttgtttgctt
    12361 atgtttataa caatgcagtt cgttcatttg tgtttattaa atctgtaaac ttattgcaag
    12421 caatacattg cgctataaaa ataaatggca ataaatactt gtttaccgtt gacggcacta
    12481 gtattgaaat gccgagaaca gcaagtgtag ctgatggtta taagttgttt ccatactttg
    12541 gtggcgatga atttgcacca catgaaataa gaatcaggat taaagatatg taagtagtaa
    12601 actcaaatct ttagtagttt tttcgataaa aatataactg taaataagat ttttttcaga
    12661 aaaataattc aaatagaact atctgctata gtttggtgct tcgtttgtaa tcgtcacatc
    12721 atgcggatga ctttcattta cgccagcagt agtaattttt acaaactgtg ctgttgcctg
    12781 tagtgatgga atatctgctg caccgcaata gcccatacca gcacgcaagc cgcccacaaa
    12841 ctgataaacg atttcactca acgtgccttt gtatggtacg cgtcctacga tgccttcagg
    12901 tactaatttt ttgatatcgt cttctacgtc ttggaaatat ctgtctttgc tgccttcctg
    12961 catggcttcc aaactgccca taccacggta ggtcttgaat tttctgcctt caaaaattac
    13021 tgttttgcca gggctttctt cagagccagc aaacaaagaa cccgccatta ttgtgttggc
    13081 acctgctgcc aatgctttta ccatgtcgcc agtataacga ataccaccgt cgccaataat
    13141 aggaacgcca gtgtttttta aagccaatga tgcatcatag attgcactga gctgcggaac
    13201 gccaacgcca gctactacac gtgtggtgca aatagaacca ggtccaacgc caacttttac
    13261 tgcatctacg ccagcttctg ccagcgcttt tgcgcctgcg cctgtagcca cattgccacc
    13321 tacgatatct aattctttga aagtttcttt tgccttttta atggaagaca atacaccttg
    13381 tgaatgtcca tgtgcagtat ctatgcaaat aaaatcaacg cctacggcaa ctaatgcttc
    13441 catgcgattc atcatgtctg cagtaacacc aacagccgca gctacgcgca atctgccgga
    13501 attatctttt gtagagttcg gataattttt tactttcaaa atatctttgt aagtaattaa
    13561 accgaccagc ttattgtttt tatctacgat aggcaatttc tcaattctgt attgatgtaa
    13621 aatctcttct gccgctttta aagttgtttt ttcaggggcc gtaatcaatc tttctttggt
    13681 catcaaatcc tgaataagca tatttttgtc tttgcaaaaa cgcaaatctc tattggttaa
    13741 aatgccaacc aaaatgccac tttcattagt aatcggtatg ccaccaatcc tattttccga
    13801 catcaatgcg aacgcatcag ctacttttga tgttggtttt aatgtaatcg gatcagtaat
    13861 taagccactt tcagagcgct taaccttgcg tacttctgct gcttgagcat ctatagacat
    13921 gtttttgtgt aaaacaccaa tgccaccttc gcgcgccaat gcgatggcta agtttgcttc
    13981 tgtaactgtg tccattgcag cagataaaat tggaatatta atgctgatat tacgtgttac
    14041 ttgcgttcgt aggttggttt ctcttggtaa aatttgagaa tatgcaggta ataacaagac
    14101 gtcgtcaaat gttaaacctt cgcctgcaaa tttgtttgaa gaaaaatgag atagtgtttc
    14161 catgtgcaaa aatacgcttt tttttatgaa ataaaaattt cgtatgattc tgccttgtaa
    14221 attaagaatt acgaaataat taggtaaaaa gcaggaaata agttgcctgt ctaaatcgtt
    14281 tgcaagtcaa aacaaatgct tctttccctt tttacttaga tgatgtatta tagtttccct
    14341 ttagcttgct ccaatacctt ttgcaatttt tctatattgt caattggcaa tatcgtcaat
    14401 gttttttcat ttgacgtgtt ggtttgtgtt gcaaaattcc agtcgagatt attttgtgca
    14461 tttacgacca tagcaagtgt tatttgaaaa ccgctcatcg tagcaaattt aatctctgtg
    14521 taatttgaag gtaatgtctt gtttttcacc atggttttca tgtattgcaa gcttactatc
    14581 aaaccatcaa tttccgattg gtcgatatag ttgtatgtgt ttttatagac tttcccttct
    14641 tgcactcgat gtacaatgcg caaacctgtg ctaatattga acttctccaa attggtgatt
    14701 tttacggctt ctattctaaa tgctggaata ttatccacag aaaatatttc ttgttttatt
    14761 aatgaatttt tggtaagaat ggcttcttct atttttgtgc gattttcttg cgcattgaca
    14821 gaagtaaaga aagtgtataa caatgcaaca acaaataatt ttttcatgaa aatattttta
    14881 tggatcaaag atacaaataa gtccaaagcg aaatatttta ttaagataaa taacttaggt
    14941 attcattttc gacagcacgg gctgcttttt tggctggtaa ataggcagca atacagctaa
    15001 tacaaaatac aacagcaccg acgattaaaa aatcggaagc catcattttt actggataat
    15061 aattgactaa gaatgaagtg ccaggcattg gaattaaacc aacagtttgc tgtaataggc
    15121 agagtaaaaa tgccaataac atgccaatga gtgcgcctgc acatgacaat aaaatacctt
    15181 caaaaaggaa aatgcgaact atgctgtttt tttgaatacc taatgcagta agtgtcgcaa
    15241 tatctttttt cttttctaat accaacatcg ataaggctcc aataatatt
//
LOCUS       DAOQQV010000061        15275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1254_length_15275_cov_9.272076, whole genome shotgun sequence.
ACCESSION   DAOQQV010000061 DAOQQV010000000
VERSION     DAOQQV010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15275
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_15275_cov_9.272076"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLL59_07570"
     CDS             <1..322
                     /locus_tag="PLL59_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23704.1"
                     /translation="RAQDTAKAIARAIHYDVQKIQTEPSIYFGTSTSIVEKIKTLPNS
                     VNDVFIFGHEPILSSLIYQFTKNVVEKFPTCAVCRIAFDINDWATLKLGKTEFFIFPK
                     LLQA"
     gene            444..2510
                     /gene="ppk1"
                     /locus_tag="PLL59_07575"
     CDS             444..2510
                     /gene="ppk1"
                     /locus_tag="PLL59_07575"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HMY23705.1"
                     /translation="MPSRLPIINRELSWLSFNERVLQEAESTDVPVLERLKFLGIYSN
                     NLDEFFRVRIATIKRMMDFDKRAKQELGFSPSKIMDAIQEKLKVLQKKYAKAYENIIK
                     ALEKENIFILNETQLDKQQGIFVKNYFQEKVRLEIFPIMLDKAKEFPILLDASIYLAV
                     RFSKNKTKETHYALIEVPTSSLSRFVVLPAKKEKQYILFLDDVIRYNLKSIFQIYDFD
                     KIEAYTIKFTRDAELDVDNDVSLTFMESMEMSLKKRKHADALRFVYDAQMPKDFQNYL
                     IKKMELDSNVQLYAGARYHNFKDFIKFPSLGKSHLLNAAQQHIPHHDLEKTRSILDKM
                     KLKDILLYFPYHSFDYFLDLLREAAIDPNVSHIRITLYRVASQSHVIKSLITALHNGK
                     DVTVFIEIRARFDEEANLDWANKLREAGARIIPGISGLKVHSKLCLITRREKGNKVYY
                     ACIGTGNLHEKTARIYTDAMVLTCNQTITKEARQIFSFFDKNYSVPKFNQLLVAPFNL
                     RSKLTTLINKEIKNAKAGKKAEIFLKLNNLVDEDVIERLYNASKAGVKIRLIVRGINS
                     LVPQFSGFSENIEAISIVDKFLEHTRIFVFHNDGKPEVFIGSADLMTRNLDFRIEVVL
                     PIIDEKIKKQLLKIMELQWKDNVKSRWFDEGQKNIFKIKTEQEAVLRSQDAIYDFWKS
                     LSEKKK"
     gene            2658..4508
                     /locus_tag="PLL59_07580"
     CDS             2658..4508
                     /locus_tag="PLL59_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HMY23706.1"
                     /translation="MKLQKLVFFALICLFLTNCKKYSSDSDRETKEKLSFQSFKTMEA
                     VANRFIEIGDSMNVAPPVALYYASLWAQLQPEVEYAFTLDSAFLIIHMKSGLEKTIAL
                     RKLDVDGLSVYRGGTGGTGHVSRISSFDSKCSHKIENKKILLYAAYEYQFYSGNEFQT
                     RVVDKIKNGDVADINVTVLKNEQCTIDAMLTFNQYGLVILDTHGYPSSIYTGIRFNLD
                     TFDIPENVDKFLTILGQKIGTNYVHSIIDAQIDFNRVFEYDPNLQNQEQWEEYKQNLQ
                     RSYDVSVTSKGIRELMPDLSQTIIFANACYSGWTATEWTKPGGTHKFKNSDPIKLAWM
                     SRNPLVFYGYESTQGGVSYQAPNKEFCKPNEDTLIKSFFYDGDSTGNAHLSEQLGGAF
                     FIEFPWNINIGMISGNHGPLRFNHYASPSWCYGNCGDSVKDTRDGKKYPTVCIGDQVW
                     MAKNLDYAGAGVCYDGNIYSCDTYGRLYTWNEVTAGVSSNASPSGIKGICPNGWHVPS
                     LSEWQKLINYVGGDVAARDKLRANSPLWEEEGTDDYGFALLPAGQCERDINSGEHVCF
                     NLDDEVSLWTTSKEDVSSLPVYFLVSDGLANEFVQNSDVTDYRSCRCVKD"
     gene            4585..5484
                     /locus_tag="PLL59_07585"
     CDS             4585..5484
                     /locus_tag="PLL59_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HMY23707.1"
                     /translation="MKFAAIDIGSNAVRLIFINVYETPSGPQFVKDAMYRVALRLGEE
                     AFLKGKISKAKETEMLSTMKAFKHLIDIHKPVQVMACATSAMRDAKNAKEIVQKIYKK
                     TGIDIKIISGQKEAELILSNHVEKFSLEENKNYLYIDVGGGSTELILLSNGKLVNKWS
                     FNIGTLRMTLDKVKPKHWKALDLWIDHLKGKYQPLLGIGSGGNINTIQKHFVKRKGEV
                     VHLEELQNTYNTLRSMTLDERILQYSFRPDRADVIVPAAYIFMYIMKRLDMDILHVPK
                     VGLGDGMIHEMYDKISKQKTKRK"
     gene            5532..6008
                     /locus_tag="PLL59_07590"
     CDS             5532..6008
                     /locus_tag="PLL59_07590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMY23708.1"
                     /translation="MKLLDNITEKLDNKYITLFAIVIGAFFLRLYRLTFEGLWTDEMA
                     AVYTSNPDVSLRRMYNVLHFWDQTPPLYPLMLRYWFQITGFTDYNARLFSVIGGTLSL
                     IAVFYLIKELYNRRTALFITLLVAITPYHIYFSREARSYIWAFLSVTMLLLFFYRQ"
     gene            5957..6994
                     /locus_tag="PLL59_07595"
     CDS             5957..6994
                     /locus_tag="PLL59_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23709.1"
                     /translation="MGISICYNAVVVFLSPIKRYSNGYNRWLFILSGGILLQISYFSF
                     FVHAALVAVVVLTFMLKKEKVDLKNWIIDYVFIGIQFLPWAYQFVKIFGMQNGGNGTS
                     PSPMYLFEILNVFTAGVAGGFLLSIAFAVLLVLFVYLVWFQKKLFKNEYLWITALVLF
                     FIIFVLFYLKSVSGRNILNGFMYSYVIVMYPVFLILLGGILYLTNRMLVGVLLLVFIT
                     ANAIHFTDWYHLSFEKKKTEAYREMADFISKSENSKAPIVCAADYLQDFYYTQKNMQN
                     QLISLSDFDKKLDTSKTTYFWIIDSYDYKSELILAEAKKRFPVRVVEIDTIKPPIGHL
                     DFRALLIEKTN"
     gene            complement(7012..8964)
                     /locus_tag="PLL59_07600"
     CDS             complement(7012..8964)
                     /locus_tag="PLL59_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23710.1"
                     /translation="MPYRLLQKILSYTATPISKVLLFLMSITYSTFLFSQDSIPISLP
                     VNIDSSTSTITVIDTIAHSNRNDSLKNDKKEARAEKTKKVFKYMSYFNYNDRGYGKDF
                     SGIEKYLPYAGKKITCIDVVIFRPFGAEADGSYTATTKGQKFGNAIHFKSREWYIKGD
                     IFFKEGDTINPALFADTEKLLWERRKFKEVKLLIIPDSLDQNNAEVVLFVQDRLSWSM
                     SVGYYNQRVVMNMSTFNFFGLPHTLSVFTGINFNKHNLWAVGGTYKYENIQASQVNFI
                     TEFVVEKLNQKVAVSLNRSFFNLKTAFAFNAKYVFDNTTISLNGNIRDPSSYVRAKSN
                     FYSLWLASAVAANKVMPCKDDKLKFIFATKLNYLDYKNRPFLINKNYNELFIKQQNYL
                     FGFGIARWDYYLEKSAYYIDVAEYFPRGISASLWAGLQIDEVYGKRAAIDLSVNYGIY
                     VKRFGYLYPQASYNGFVRNKKGEQMKANFQVDYVSKQVSFARYMKFRQIIKAGTTLGF
                     YIPEERYFNINDMKGIRGFYSPTLKGSQSVTLSLESNLLFNKTVAMSKGMLYAFCDMG
                     WLSENGEKLIVESDFQYGIGAGIRLRSVDLGFPYIDFQFGFYPKGKSFGAQLFQFKLY
                     EQNNYAVLQNNMFVETPMNYNAKPSY"
     gene            complement(8980..9621)
                     /locus_tag="PLL59_07605"
     CDS             complement(8980..9621)
                     /locus_tag="PLL59_07605"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HMY23711.1"
                     /translation="METIKHAIDFVLHPDLAYLLQTYGVYIYAILFLIIFVETGLVIM
                     PFLPGDSLLFTAGMLTVIEPGLNIYVLIPLLLVAAVTGDTLNYFVGKYFSDRVLQWRF
                     RGKPLVQKKWIDDTHEFFEKHGNKTIVLARFVPIVRTIAPFVSGIGKMTYSTFTVYNI
                     FGAFLWIVSMVMLGHFLGSNAFVKENLEKFVLGIVFLSVLPMLIKFIQVRMKK"
     gene            complement(9689..12535)
                     /locus_tag="PLL59_07610"
     CDS             complement(9689..12535)
                     /locus_tag="PLL59_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HMY23712.1"
                     /translation="MNTMYKPIYLLLILMLLSISILSAHEGCNLPLSEKSNRIKVKMP
                     ATVEERLEIIDALEIDHYYIDNGYLITEVSETNANKLKSLPFEYDIVCSDVAAELKKE
                     NDAYYKAIRENSNARVAFENSNDTLGNIIKKPAAFQVLSTLGGYYSYAQMVTAMGNLI
                     NTYPTIVDTFSIGKTYEGRDIWCVKISDNVLTDENEPELGYMGLQHAREAIGGASMIF
                     FMQYLCENYSTDNRIKDLVDNREFFIIPCMNADGWEYNRSTNSSGGGGWRKNRRPFGS
                     GNYGVDLNRNWSVRWADCAGAMGASSCGSNSSSSETYWGTAAFSEPETQAVRAFTKTR
                     NFSIFLDQHAYGPYYSIPPGRVADALSPAGLKFYKELCANMGKYNGMKYGNSYEALAY
                     EVAGGVKDWMLSGEIGVGTKGKVYGFTGEGGAGGGTGGSYGNFWPPANKIVILCKGMT
                     YQNLQMAYTAGSYVDHQDLGNISVTTKTGNLPFRLRRLGLQNRPVTVSIIPLQNMSVG
                     AAVTTSLTNFNDTYDGNISYTLPAAITTGHVIKYAWKIETEAMVYYDTIAKIFNGVSE
                     IADNMESGSVSANWTVTAGSGASPTWAYSSAYSYAGSRSLANAPAAPTTKYGTSAVTR
                     TAQFNTNMNLSGSGSAYLSFMVKHRAENFCDKLQVQISTNGSTWTALSGKTTVKEPNN
                     WDGSTINGEPALTGIREEWTREIFDLQNYVGLNNLRFRFVFTTTNTDANYVFNTDEGF
                     FIDNFEVMSGGNSNVILPLELISFTGKNIEEGNLLNWTTASELNTTHFEIERTVNGVD
                     FETLGSVAAAGNSKSIQQYNFIDKQPYLGENLYRLKMYDLDGSYSYSKMIAIEVHDAT
                     AFTFTPTGIEKLFPNPTNANLNVLFNVSENIASFNLQAYDITGAVLYQQQLQLEKGKH
                     TIPIDVSILSTGQYILSITNPINNVTYEQKFIKQ"
     gene            complement(12577..15087)
                     /locus_tag="PLL59_07615"
     CDS             complement(12577..15087)
                     /locus_tag="PLL59_07615"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMY23713.1"
                     /translation="MNTFLHKLFVVICMLSAILNNKIAKAQCGGTYSPVTLNWDYQYY
                     NNSDLPSSPINFMMGKNTLSMSWSGATFNGVNNNHTNHTNSYGNGNDIQFSLRNGSIT
                     FTFLQPVENLRFTIHDVDNRHRVDPKGYNASNVQVPVVMTRVSGTAGSLVGSGGNNPY
                     YYNSAAYALNSTGSSVNVSIAGPLSRVTLNFTKSTTGSDPVYISDIQACTNSTWATDY
                     QAISAPETGQPTHMLVAYDSIMYVVNKLDNTAMQLFNDPSMTRLNTVAYDPYNQIIYY
                     CNSARVSSNRSVYKYDVKTGTKSTFIADVNTLGIQTFAQGLATAGAGFYDGFLFLGTD
                     SDLYDWTPAAIYRIDIDNSGTPIRASRYWGVNSAIPTGGGGTDILYDWGDFVINDGVL
                     YNFNGGSDAAANTELEHFDLNAQSKLAGYSFSPDTMAQASLDYEGNIFSIRNGFYYQQ
                     YNMDGTFGARNYYSGILPTRVLCDGAESFKYPYDYGDAPSTYGYASHVFRVSPNLRIG
                     STIDYEMSSFYSSDASADNSDVSDDEDGVNPSYFTSNPLTASHTNYTVPITISNTSGA
                     NAYLYGYLDFNSDGSFTNIGERSGIITIPTGTINGSFNVTWTGLTGGSAGSSFIRFRL
                     SNDSTELRYGSGYARSGEVEDYPINITPSFLPVELVSFTGEALENNTTLLKWSTASEF
                     NNQYFEVQRQDANTWETIGVENGKGNSQQLQNYTHIDAQPKEGINYYRLKQVDFDGKF
                     EYSPVISVKFSNISNSPSTTPAVSIYPNPTSGEVWVKTDENIPSDNPQNISVFDVKGQ
                     QILSSLHKENTQLVDLKYFENGLYYLKIGKQSYKIIKH"
BASE COUNT         4944 a         2599 c         2633 g         5099 t
ORIGIN      
        1 gcgtgcgcaa gacactgcaa aagctatcgc tcgtgctata cattatgatg tgcaaaaaat
       61 ccaaacggaa cccagtatct attttggcac ttcgacaagc atcgtcgaaa aaattaaaac
      121 attaccaaat agtgtgaatg atgtttttat atttggacat gagccgattt tgtcttcttt
      181 gatttatcag tttacaaaaa acgttgttga aaaatttcca acttgtgctg tatgtcgcat
      241 tgcgtttgat ataaatgact gggcaacatt aaaactcgga aaaacggagt tttttatttt
      301 tccaaaatta ttgcaagctt aaaccataag ctcaaaaaca ataaattata gtaattgtga
      361 attttaggtg aacgtacatt tttactatca tttcaggaac atattcttct atatttacgt
      421 tataattttt tagatacttt tttatgccgt ctcgtcttcc tattatcaac cgcgaattaa
      481 gctggctttc ctttaacgaa cgtgtgttgc aagaggcaga aagcactgac gtgcctgtgc
      541 tggaaaggct aaaattttta ggcatttact ctaataattt agatgaattt ttccgcgtgc
      601 gcatcgctac tattaaacgc atgatggatt ttgacaaacg tgccaagcaa gaattgggat
      661 tttcgccttc caaaataatg gatgccattc aggaaaaact gaaagtgctg caaaaaaaat
      721 atgcaaaagc ctatgaaaac atcatcaaag ctttagagaa agaaaatatt tttattttaa
      781 acgaaacgca attagacaaa cagcaaggca tatttgtcaa gaattatttt caggaaaaag
      841 tacgactcga aatttttcca attatgctgg ataaagccaa agagtttccg attttattag
      901 atgcatctat ttacttggcc gtgcgttttt caaaaaacaa aacaaaagaa acacactatg
      961 cgttgattga agtaccaaca agctcattgt cgagatttgt cgtgctgcca gcaaaaaaag
     1021 aaaagcaata tattttgttt ttagatgatg tgattcgcta taatttaaaa tccatttttc
     1081 agatttacga tttcgataaa atagaagctt acaccataaa gtttacgcgc gatgcggaat
     1141 tagatgtcga taatgatgtg tcgctcacct ttatggaatc tatggaaatg agtttgaaaa
     1201 aacgcaaaca tgcagatgca ttgcgttttg tatatgatgc acaaatgcca aaagatttcc
     1261 aaaattattt aataaaaaaa atggaattag atagcaatgt acagctatat gctggcgctc
     1321 gttatcataa tttcaaagac tttattaaat ttccttcgct cggaaaatcg caccttttaa
     1381 atgcagcgca gcaacatatt ccacatcacg acttagaaaa aacacgttct attttagaca
     1441 agatgaaatt gaaagatatt ttactatatt ttccatatca ttcattcgat tattttttag
     1501 accttttgcg cgaagctgcc attgatccaa atgtatcgca catccgcatt acgctatatc
     1561 gcgtagcatc tcaatcacat gtcataaaat ctctgattac cgcattgcat aatggaaaag
     1621 acgttaccgt gtttattgaa attcgtgcgc gatttgacga agaagctaac cttgattggg
     1681 caaacaaatt gcgcgaagca ggcgctcgta ttattcctgg aatttctgga ttgaaagtgc
     1741 attcaaaatt atgtttgatt acgcgcagag aaaaaggaaa caaagtttac tatgcctgca
     1801 ttggcactgg aaatttgcac gagaaaactg ctcgcatata tactgatgca atggtgttga
     1861 cttgtaatca aacaataaca aaagaggcac gacaaatttt ttctttcttt gataaaaatt
     1921 attctgttcc aaagttcaat cagttgttag tggcgccttt taatttgcgt agtaaattga
     1981 ccacactcat caacaaagaa attaaaaatg caaaagccgg caaaaaagcg gagatatttt
     2041 tgaagttgaa taatttagta gatgaagatg taattgaaag attgtacaat gccagtaagg
     2101 ctggtgtaaa aatcagatta atcgttcgcg gcatcaactc cttagtgcca caattttctg
     2161 gatttagcga aaacatcgaa gccatcagca tcgtcgataa atttttagaa cacactcgta
     2221 tttttgtatt tcataacgat ggcaagccag aagtatttat tggttctgcc gatttaatga
     2281 cacgcaatct tgacttcaga atcgaagtag tattgccaat aatagatgaa aaaataaaaa
     2341 agcaattact gaaaataatg gaattgcaat ggaaagacaa tgtgaagtcg agatggtttg
     2401 atgaaggaca aaaaaatatt ttcaaaatca aaacggaaca agaagccgta ttgcgcagtc
     2461 aagatgcgat ttatgatttt tggaaaagct tgagtgagaa gaaaaaataa tcattaatag
     2521 tcaacagatt ttaatagtcg tattggcttg ttaatcataa gattttttaa gttggcttaa
     2581 tttcattggt ttaatgaaag cttagggtgc tattgtaaat ctcgggtaaa aatattacat
     2641 tgtattaaag caaaaccatg aaactacaga aattggtatt ctttgcacta atttgccttt
     2701 ttttgacaaa ttgtaaaaaa tattcttcag attctgatag ggagacgaaa gaaaaactct
     2761 cctttcaatc atttaaaaca atggaagctg ttgctaatcg attcatcgaa attggtgact
     2821 caatgaatgt tgcgccacca gttgcacttt attatgctag cctatgggca caacttcagc
     2881 cagaagttga gtatgcattt actttagaca gcgcttttct aatcattcac atgaaaagtg
     2941 gcctagaaaa aactattgca ttacgtaaat tagatgttga tgggttaagt gtttatcgcg
     3001 gtggtactgg tggtactgga catgtttcaa gaatttcttc atttgacagc aaatgttccc
     3061 ataaaataga aaataaaaag atattactat atgcagctta tgaataccaa ttttattctg
     3121 gtaatgaatt tcaaactcgt gttgtggaca aaattaaaaa cggtgatgtg gcagatatta
     3181 acgttaccgt tcttaaaaat gaacaatgta ctattgatgc gatgttaact tttaatcaat
     3241 atggtcttgt aattttggac acacatggat acccgtcttc aatatatacg ggaataagat
     3301 ttaatcttga cacctttgat atacctgaaa atgttgataa attcctcaca atattaggtc
     3361 aaaaaattgg cacaaattat gtccattcga taatcgatgc tcaaattgac tttaatagag
     3421 ttttcgaata tgacccaaat cttcagaatc aagaacaatg ggaagagtat aaacaaaact
     3481 tgcaaagatc ttatgatgtt tctgttactt ctaaaggcat acgtgaattg atgccagatt
     3541 taagtcaaac aattattttt gctaatgctt gctatagcgg atggactgcc actgaatgga
     3601 caaagccagg aggtactcat aagtttaaaa acagtgaccc tataaaatta gcttggatgt
     3661 ctcgaaatcc tttagtattt tatggatatg aatcaactca aggtggtgta tcttatcaag
     3721 ccccaaataa agaattctgc aagccaaatg aggatacact tataaagtct tttttttatg
     3781 atggtgatag tacaggtaat gcacatctta gtgaacaatt aggtggggcg ttttttattg
     3841 aatttccatg gaatataaat atcggcatga taagtggcaa ccatggacca ttacgtttta
     3901 accattatgc aagtccatct tggtgctatg gcaattgtgg cgactctgtc aaagataccc
     3961 gagatggaaa aaaatatcca acggtatgca ttggcgacca ggtgtggatg gcgaaaaacc
     4021 tagattatgc aggagctgga gtttgctacg atggtaatat atactcatgt gacacctatg
     4081 gaaggcttta tacatggaat gaagtaactg ctggtgtttc ttcaaacgct agccctagtg
     4141 gaataaaagg aatatgtccc aacgggtggc acgttccttc tctctcagaa tggcaaaaac
     4201 ttattaatta tgtaggagga gacgtagctg ctcgggacaa attgagggct aactctccgc
     4261 tttgggaaga agaaggtaca gatgattatg gatttgcact tttgccagct ggtcagtgcg
     4321 aaagagacat caatagcggg gagcatgttt gttttaactt agatgatgaa gtaagcttat
     4381 ggaccacttc caaagaagac gtctcctccc ttcctgtata ttttttggtt tcagatggtt
     4441 tagccaatga atttgtacaa aatagtgatg ttactgatta tcgttcttgc cgctgtgtga
     4501 aggattgatt ttaaaaaaaa cttacttttt tctagtagtg gcttatcagt tgaattgaat
     4561 aaaataaaat cgtaaattgc ccgcatgaaa ttcgctgcta ttgatattgg ttccaacgct
     4621 gttcgcttaa tttttataaa tgtatatgaa acaccatctg gtccgcagtt tgtaaaagat
     4681 gccatgtatc gcgttgcact tcgccttggt gaagaagcat ttttaaaagg aaaaatttct
     4741 aaagcaaaag aaacagaaat gctatctacg atgaaagcat ttaagcattt gatagatata
     4801 cataaacctg tacaagtgat ggcatgcgca acgagtgcca tgcgcgatgc aaaaaatgca
     4861 aaagaaattg tacagaaaat ttataaaaaa accggcattg atatcaaaat tatttctggg
     4921 cagaaagaag cagaattaat cctgtcaaat catgtagaaa aattttcatt agaagaaaat
     4981 aaaaactatc tatacattga tgtcggcggt ggctctactg aattaatttt attatccaat
     5041 ggaaaattgg tcaataaatg gtcttttaat attggtactt tgcgcatgac attagataaa
     5101 gtaaaaccaa aacattggaa agctttggac ctatggattg accatttaaa aggaaaatat
     5161 caaccattat taggcattgg ttctggtgga aatatcaaca ccatacaaaa gcattttgta
     5221 aaacgaaaag gcgaagtggt gcatttagag gaattacaaa acacctacaa tacacttcgc
     5281 agtatgacac tcgacgagcg cattttacaa tatagtttcc gacctgatag agctgacgtt
     5341 atcgtgccgg ctgcttatat ttttatgtat atcatgaagc gtttggatat ggatatttta
     5401 catgttccaa aagttggatt gggtgatggt atgatacatg agatgtatga taaaatatca
     5461 aaacaaaaga ctaaaagaaa gtagcttatt tttttaagca aattgtctta cctttaattt
     5521 caaatacacc tatgaaatta ttggacaaca ttaccgaaaa attagacaac aaatacatta
     5581 ccttatttgc tatagttatt ggtgcatttt ttttgcgctt gtatcggcta acttttgaag
     5641 gtttatggac cgatgagatg gcggcggtat atacttcaaa tcctgatgta tcattgcgaa
     5701 ggatgtataa tgtattacat ttttgggatc aaacgcctcc gctatatcca ttaatgttgc
     5761 gatactggtt tcaaatcact ggatttactg attataatgc gcgtttattt tcagtaatag
     5821 gcggcacatt gagtttgatt gctgtgtttt atttgataaa agaattatac aataggcgaa
     5881 ccgcattatt tattacctta ttggtagcca taacgcctta tcatatttat ttttctagag
     5941 aagcgcgttc atatatttgg gcatttctat ctgttacaat gctgttgttg tttttttatc
     6001 gccaataaaa aggtattcaa acggatataa cagatggcta tttatattga gtggtggcat
     6061 tttattacaa ataagttatt tcagcttttt tgtgcacgca gctttggtgg ctgtggtcgt
     6121 gctcactttt atgctaaaaa aagaaaaggt agatctaaaa aattggatca tcgattatgt
     6181 attcattggc atacagtttt tgccatgggc ttatcagttt gtgaaaatat ttggcatgca
     6241 aaatggcggc aatggcacat caccaagtcc aatgtacttg tttgaaatac tcaatgtgtt
     6301 tactgctggc gtagcaggtg gtttcctatt atcaattgca tttgcagtat tattggtgtt
     6361 atttgtttac ttagtatggt ttcagaaaaa gttatttaag aatgaatatt tatggataac
     6421 tgctctcgtg cttttcttta tcatattcgt tttattttat ttaaaatctg tttctggtag
     6481 aaatattttg aatggcttta tgtatagcta tgttattgtc atgtatcctg tttttcttat
     6541 tttattaggt ggcattttgt atctaacaaa cagaatgtta gttggtgttt tattgttggt
     6601 gtttattact gcaaatgcaa tacattttac ggattggtat catctttcat ttgaaaaaaa
     6661 gaaaacagaa gcctatcgcg aaatggctga ttttattagt aaatctgaaa acagtaaagc
     6721 gcctattgta tgtgcggcag attacttaca agatttttat tacactcaaa aaaacatgca
     6781 aaaccaactc attagtttat ctgattttga caagaaatta gacacctcta agactaccta
     6841 tttttggatt attgactctt atgattacaa atcggaattg attttagcag aagcaaaaaa
     6901 acgttttccc gttcgtgtcg tagaaataga taccatcaaa ccaccaattg gtcatttgga
     6961 ttttcgcgca ttattaatag agaaaacaaa ctaaattaat cagaaaagtt tttaatagct
     7021 tggtttagca ttgtagttca tcggcgtttc tacaaacata ttattttgta agacagcata
     7081 attattttgt tcatacaatt taaactgaaa taactgtgca ccaaatgatt ttccttttgg
     7141 ataaaatccg aattgaaaat cgatatatgg aaatcctaaa tcgacacttc ttagacgtat
     7201 gccagcacct atgccatatt gaaaatcgct ttccacaatt agtttttcgc cattttcgct
     7261 gagccagccc atatcacaaa atgcatataa catgccttta ctcatggcta ctgttttgtt
     7321 aaacaaaagg ttactttcca agcttaaggt aacactctgt gagcctttca aagtcggcga
     7381 ataaaatccg cgaatgcctt tcatatcatt gatattaaag tagcgttctt ccggaatata
     7441 aaaacccaat gtagtgcctg cttttatgat ttgtctaaac ttcatatatc ttgcaaaaga
     7501 cacttgtttt gacacataat cgacttgaaa atttgccttc atctgttcgc cttttttgtt
     7561 gcgcacaaat ccattatatg atgcttgagg atataggtaa ccaaatcttt tgacataaat
     7621 tccataattg accgacaaat cgatggctgc tcttttgcca tatacttcat cgatttgcaa
     7681 accagcccaa agtgatgcgc tgatgcctct aggaaaatat tctgcaacat cgatatagta
     7741 agcgcttttt tcgagataat aatcccaacg cgcaatacca aaaccaaata gataattttg
     7801 ttgttttata aacaactcat tatagttttt attgattaaa aatggacgat ttttatagtc
     7861 taaataattg agtttcgtgg cgaaaataaa tttgagttta tcgtctttgc aaggcattac
     7921 tttattggcg gcaactgcac tagccaacca tagcgaataa aaattcgact ttgctcgcac
     7981 ataactcgat ggatctctga tattgccatt taggctgatg gtggtattat caaaaacata
     8041 ttttgcgtta aatgcaaagg cagttttcag attgaaaaaa cttctgttta aggaaacggc
     8101 tactttttga tttaattttt ctacaacaaa ttcagttatg aaatttactt gcgaagcttg
     8161 tatgttttca tatttataag taccgccaac agcccagaga ttgtgtttat tgaagttgat
     8221 gccagtaaac acagacaagg tgtgtggcaa gccaaaaaaa ttaaaagtag acatattcat
     8281 cactacgcgc tgattgtaat agccaacaga catcgaccaa gataatctgt cttgtacaaa
     8341 caataccact tcggcattat tttgatcgag tgaatctggt ataattaata gttttacttc
     8401 tttgaatttt cttcgttccc aaagtagttt ttctgtgtca gcaaacaatg ctggattgat
     8461 agtgtcgcct tctttaaaaa aaatatcgcc tttgatatac cattcacgac ttttgaaatg
     8521 aatggcattt ccaaattttt gtcctttcgt agtagcagta taagagccat ctgcctctgc
     8581 accaaatgga cgaaaaataa caacatcgat acaggtaatt tttttacctg cataaggcaa
     8641 atatttttct ataccagaaa aatctttacc atatccacga tcattatagt tgaagtaaga
     8701 catatacttg aagacttttt ttgtcttttc ggcacgtgct tcttttttgt cattttttaa
     8761 tgaatcgttg cgattactat gtgcaatggt atcaatgacc gttattgtag atgtcgagct
     8821 gtcaatattt acaggtagtg atataggaat gctatcttgc gagaataaaa atgtgctata
     8881 tgtaatgctc attaaaaaaa gcaacacctt ggaaattggt gttgctgtat aagataatat
     8941 tttttgcagt aaacgataag gcattgttta cactgtactt tattttttca ttcttacttg
     9001 tataaatttt ataagcattg gcaacactga taaaaacaca attcctaata caaatttttc
     9061 taaattttct tttacaaatg cattagaacc taagaaatgt ccgagcataa ccatgctaac
     9121 aatccataag aaagcaccaa aaatattata tacggtaaat gtagaatagg tcatttttcc
     9181 gatgcctgaa acaaaaggcg caatggtgcg aacgataggc acaaaacgcg cgagtacgat
     9241 ggttttgttt ccatgttttt caaaaaattc gtgtgtgtca tctatccatt ttttttgtac
     9301 taatggcttg cctctaaacc gccattgtaa cactctatca ctaaaatatt ttcctacaaa
     9361 ataattaagc gtatcgccag taacagcggc aactaataac agcggaatta atacatatat
     9421 atttaatcca ggttctataa cggtaagcat tcctgcggtg aataagaggg aatcgccagg
     9481 aaggaaaggc ataatcacca atccggtttc cacgaaaatg attagaaaaa gaatagcata
     9541 aatatatacg ccataggttt gcaataaata agctaaatca ggatgtagta caaaatcaat
     9601 agcgtgttta atagtttcca tagtaaaata ttcaattaaa tcggtttcta agatactgat
     9661 tttaattgtg gcaatactat aaaatatttt attgttttat aaacttctgc tcgtaggtaa
     9721 cattatttat agggttggta atcgacaata tatattgacc tgtagataat attgaaacat
     9781 ctataggaat ggtatgtttg cctttttcta attgtaattg ttgttgatat aaaactgcac
     9841 ctgtgatgtc atatgcttgt aaattaaatg atgcaatatt ttcagaaaca ttgaataata
     9901 cattgagatt agcattagtt ggatttggaa acaatttttc tatacctgtt ggtgtaaaag
     9961 tgaatgctgt tgcatcatga acttcaattg ctatcatttt actataagaa taagagccat
    10021 ccaagtcata catttttagt ctgtataaat tttcgcctaa ataaggttgt ttgtctataa
    10081 aattatattg ctgaatagat ttactatttc cagctgcagc aacagagccc agcgtttcaa
    10141 aatcaacgcc attaacagta cgttctattt caaagtgagt ggtgtttaat tcagaagcag
    10201 tggtccagtt gagcagattg ccttcttcta tatttttacc tgtaaaagag ataagttcta
    10261 aaggcaaaat aacattggaa ttaccaccag acattacttc aaaattatca ataaaaaaac
    10321 cttcatctgt attaaataca taatttgcat ctgtattagt agtggtaaaa acaaagcgaa
    10381 aacgcaaatt atttaaacca acataatttt gtaagtcaaa tatttctctg gtccactctt
    10441 ctctaatacc tgttaatgcg ggctctccat tgatagtact gccatcccaa ttattaggtt
    10501 ctttcactgt tgttttaccg cttaatgctg tccaagttga gccatttgta gatatctgta
    10561 cttgcaattt atcacaaaaa ttttcagctc tatgcttcac cataaagctt agataagcac
    10621 tgcctgagcc agatagattc atgttggtat taaattgagc tgttctcgtt actgccgacg
    10681 taccatattt tgttgttggc gcagcaggtg catttgctag tgatctagaa cctgcgtatg
    10741 aataagcgga agaatatgcc caagttggac ttgcacccga tccggcagta actgtccaat
    10801 tcgcacttac cgatcctgat tccatattat ctgcaatttc agagacgcca ttaaatattt
    10861 ttgcaatcgt atcataatat accattgctt ctgtttcgat tttccaagcg tattttatta
    10921 catgaccagt agtaatggcc gctggtagtg tataagatat gttgccatcg taagtatcat
    10981 taaaattagt caaactagta gtaacagcag cacctacact catgttttgt aatggaatta
    11041 tagatactgt tactggtctg ttttgtaaac ctaatcttct taatctaaaa ggtaaatttc
    11101 ctgttttggt ggtgacactt atattgccta aatcttgatg atctacataa gagccagcag
    11161 tatatgccat ttgcaaattc tgataagtca tacctttgca aaggataact attttattgg
    11221 ctggtggcca gaaattgcca taagaacctc cagtgcctcc acctgcacca ccttcgccag
    11281 taaatccata tacttttcct ttggtgccca caccaatttc gccgcttaac atccaatctt
    11341 ttacaccgcc tgctacttca tatgctaatg cttcataact attaccgtat ttcatgccat
    11401 tatattttcc catattggca catagttctt tataaaattt taagcctgca ggtgatagtg
    11461 cgtcagccac tcttcctggt ggaatactat aatatggtcc atatgcatgt tggtctaaga
    11521 aaatactaaa atttcttgtt ttagtgaaag cacgtactgc ttgtgtttct ggctcagaaa
    11581 atgctgctgt gccccaataa gtttcagaac tgctagaatt tgaaccacaa cttgaagcgc
    11641 ccattgcacc tgcacaatct gcccatctta cgctccaatt tctatttaaa tctacaccat
    11701 aattacctga tccgaatggt cttctatttt ttctccaacc accaccacca cttgaatttg
    11761 ttgatcgatt atattcccat ccatctgcgt tcatacatgg aataataaaa aactctctat
    11821 tatccactaa atctttgatt ctattatctg ttgaataatt ttcgcataaa tattgcataa
    11881 aaaatatcat gctggcaccg ccaatcgctt cacgtgcatg ttgcaaaccc atatatccta
    11941 gttcaggttc gttttcatct gttaatacat tatctgatat ttttacacac catatatctc
    12001 gaccttcata tgtttttcca atagagaaag tatctactat agtaggatag gtattaatta
    12061 aattgcccat tgcagttacc atctgtgcat aactataata accgcctaaa gtagataaaa
    12121 cttgaaatgc tgctggtttt ttgataatat tccctaaggt atcattgcta ttttcaaatg
    12181 ctactctcgc atttgagttt tctcgaatgg ctttatagta ggcatcattt tcttttttaa
    12241 gttcggctgc tacatcactg caaacgatat cgtactcaaa aggcaaagac ttaagtttgt
    12301 tggcattggt ttcactgact tctgttatca gatatccatt gtcgatataa taatggtcta
    12361 tttcaagtgc gtcaattatt tctaatcttt cttcaacagt agctggcatc ttcactttta
    12421 ttctattaga tttttctgat agtggaagat tgcaaccttc atgtgcagaa agaatagaaa
    12481 tagataaaag cattaatatc aatagtaagt atatgggttt gtacatagtg ttcatagtct
    12541 atatttattt gagttgggaa ttaagtaatc ttttgattag tgttttatta ttttgtatga
    12601 ctgttttcca attttcagat aatataatcc attttcaaaa tattttaaat ctactaattg
    12661 cgtattttct ttatgaagtg aggataatat ttgttggcct tttacatcaa atacgctgat
    12721 gttttgtgga ttgtcagatg gtatattctc atctgttttc acccaaactt ctccacttgt
    12781 tggattagga taaatagaaa ccgcaggtgt agtgcttggt gaattagata tattgctgaa
    12841 tttaacagat atcactggtg aatattcaaa tttaccatca aaatcaactt gtttaagtct
    12901 gtaatagttt attccttctt ttggctgcgc atcgatatgt gtatagtttt gtaattgttg
    12961 gctattgcct tttccattct ctacgccaat cgtttcccat gtgttagcgt cttggcgttg
    13021 tacttcaaag tattgattat taaattcaga tgctgttgac cacttcaaca aagtagtatt
    13081 attttccaaa gcttctcctg tgaatgaaac taattcgaca ggaagaaaag aaggtgttat
    13141 atttatcgga taatcttcta cttcaccaga tctagcgtaa ccgctaccat atctaagttc
    13201 tgttgagtca ttggataatc tgaaacgaat aaaagaactg ccagctgaac ctccagttaa
    13261 acctgtccat gttacattaa aagagccatt tattgttcct gttggaatag taattattcc
    13321 agatctttct ccaatatttg taaagctacc gtctgaatta aagtccaagt aaccgtataa
    13381 atatgcattt gcgccagatg tattgcttat tgtaatcggc actgtatagt tagtatgaga
    13441 tgctgttaaa ggattggaag taaaataaga aggattaacg ccatcttcat catcagagac
    13501 atctgaattg tctgcagatg catctgagct ataaaaacta ctcatttcat aatcaatagt
    13561 agaaccgatt ctcaaatttg gtgaaactct gaaaacgtgt gaagcatatc cataagtaga
    13621 tggcgcatca ccataatcat aaggatattt aaaagattcc gcaccatcgc aaagtactct
    13681 agtaggaagg atgcctgaat aataatttct agctccaaat gttccatcca tattatattg
    13741 ttgatagtaa aatccatttc tgatgctaaa gatattacct tcatagtcta atgatgcttg
    13801 ggccatggtg tctggagaaa atgaatagcc tgctaattta ctttgcgcat tcaaatcaaa
    13861 atgttctaat tccgtatttg ctgctgcatc tgatccacca ttaaaattat ataaaacgcc
    13921 gtcatttatt acaaaatctc cccagtcgta tagaatatct gttccaccac caccagttgg
    13981 gattgcacta ttaacgcccc aatatctgct ggctctaata ggtgtgccac tgttgtcaat
    14041 gtcgattcta taaatagctg ctggtgtcca atcatataaa tcactatctg ttcccaaaaa
    14101 taaaaaacca tcataaaagc cagcacctgc agttgccaaa ccttgagcaa atgtttgaat
    14161 gcctaaagtg ttcacatctg caataaatgt actttttgta ccagttttta catcatattt
    14221 ataaaccgat ctattagatg atacacgtgc actattacaa taataaatta tttgattata
    14281 tggatcgtat gcaaccgtat taagcctagt cattgatgga tcattaaata attgcattgc
    14341 tgtattgtct aatttattaa caacatacat gatgctatca taagctacaa gcatgtgtgt
    14401 aggctgtcct gtttctggcg cagatatcgc ttgatagtct gtagcccatg tactattcgt
    14461 gcaggcttgg atatctgata tataaactgg atctgaaccc gtagtcgatt tagtaaaatt
    14521 taaagtaacg cgagaaagag ggccagcaat agaaacattt actgatgaac ctgtactatt
    14581 caatgcataa gctgcactat tataataata tggattatta ccaccactac caactaaaga
    14641 gcctgctgta ccagaaaccc ttgtcataac tacgggcact tgcacattag atgcattata
    14701 tcctttagga tctactcgat gacggttgtc cacatcatgt atagtaaatc ttaaattttc
    14761 tactggttgc aaaaatgtga aggtaataga accatttctt aatgaaaatt gtatatcatt
    14821 accattacca taactatttg tatggttggt atgattatta tttactccat taaatgtagc
    14881 acctgaccaa gacatcgaca atgtgttttt gcccatcata aaattgattg gactgctcgg
    14941 taaatcagaa ttattatagt attggtaatc ccaattaagt gtaactggag aataagtacc
    15001 accacattgt gctttagcta ttttgttatt taatatagca cttaacatgc atatgacaac
    15061 aaataatttg tgtagaaatg tgttcattgt atagggttta atatgttatt aatttttata
    15121 gcgctttgca aatacaaaat acattttgca aattgctagt acaacacaat aaccctaata
    15181 aatatattaa aatatttaat ttaaaattgg ctaaaattta tttttttatt tatcaaatca
    15241 ataaagaatg aattttaggt tttatgtctt ttaac
//
LOCUS       DAOQQV010000062        14992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1289_length_14992_cov_7.111468, whole genome shotgun sequence.
ACCESSION   DAOQQV010000062 DAOQQV010000000
VERSION     DAOQQV010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14992
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1289_length_14992_cov_7.111468"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLL59_07620"
     CDS             complement(<1..385)
                     /locus_tag="PLL59_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23714.1"
                     /translation="MNPSYKISVVSYLNTKPFIHGLHHPSIYDNIKLSQETPAQCAQK
                     LLSGDIDIGLVPIAVIPQLKNPRIVSPFCIGANGAVKTVCLFSDVALEDISTVYLDYQ
                     SRTSVLLVQVLMKEFWKKEVSFLPAI"
     gene            complement(415..1041)
                     /locus_tag="PLL59_07625"
     CDS             complement(415..1041)
                     /locus_tag="PLL59_07625"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMY23715.1"
                     /translation="MQTSNTKKEIYIIRHGQTDFNVKQIVQGRGINSDINDTGIQQAK
                     LFFEAHKEIKFDIVYTSSLKRTWQTVDSFISKKIPHLIRTNLDEIDWGIFEGVEHHAG
                     LQKEYYDIIEEWKNGNLSVKIEGGESANDLAERLLPFIEEIKNTTAQTILVCTHGRTL
                     RILICLLLRIDIAQMDNFDHQNTCLYHLSYDDNTFSLIKENDTTHLNV"
     gene            complement(1195..2199)
                     /locus_tag="PLL59_07630"
     CDS             complement(1195..2199)
                     /locus_tag="PLL59_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMY23716.1"
                     /translation="MIKIGIIGIGRLGVQHLERLLISPYFKVIGCFDKEQHKLKPIQE
                     EYGVDCFDDVDELINKCDAVEILTPSDSHYYYAEKAIRFGKHVYVDKPISNSADEAKK
                     LVELIREAGIIFQVGHTERYNPVFLALKQQSIQPLFIESHRLSLFDAKRTNDSIIMDV
                     MIHDLDIILSIVKANIKSISASGVSVLSDQIDIANARIEFDNACVANLTASRTSVQKM
                     RKMLFFQKDSHITLDFLNREAELLHLSETAKHNAIPMHIQETTRYINTETMVTEEYDA
                     LKEELNSFAECLLFNKEPNVTALDSLKSIEVASEILQKIQKMNSHLELSKLPVNTMVG
                     "
     gene            2515..3822
                     /gene="mtaB"
                     /locus_tag="PLL59_07635"
     CDS             2515..3822
                     /gene="mtaB"
                     /locus_tag="PLL59_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HMY23717.1"
                     /translation="MISDKKIAFHTLGCKLNFSETSAITRQMQARGFSTVDFEEVSDV
                     YVINTCSVTEQADTKCRNIVRKALKLNPDAYVVVIGCYAQLKPKEIASIEGVDLVLGA
                     AEKFKLPEIITDLTKNPCGQVLASEISNANFFVDAYSVGDRTRSFLKIQDGCDYKCSF
                     CTIPLARGKSRSDTPENILKNAKDLAAKGVKEIVLTGVNTGDYGKGIASESNFFDIVK
                     MLDEVEGIERYRISSIEPNLLTDEIIEFVANSKRFMPHFHIPLQSGSDKMLKLMQRRY
                     KRDLYKSRVEKIKSVMPHACIGVDVIVGFPSETEDDFLETYQFINALDISYLHVFTYS
                     ERQNTKALEIEGIVPMPIRNERNAMLRSLSEKKKRYFYQQFLGQAKTVLLEQEQKGNI
                     MHGFTDNYIKISVPYHAASVNQMATAQLQSIDDAGDVLAILID"
     gene            complement(4073..5473)
                     /gene="fumC"
                     /locus_tag="PLL59_07640"
     CDS             complement(4073..5473)
                     /gene="fumC"
                     /locus_tag="PLL59_07640"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HMY23718.1"
                     /translation="MQYRIEKDTMGKVNVPIDAYYGAQTQRSIENFQIAQDINKMPKE
                     IIRAFAHLKKAAAITNYKAKVLDKQKCDTIGKVCDEILAGKLDDQFPLVVWQTGSGTQ
                     SNMNVNEVIAYRGHVLLGGQLTDEKKLINPNDDVNKSQSSNDTFPTAMHIAAYTILME
                     KTIPGIKKLRNTLAKKSKEFAKVVKIGRTHFMDATPLTLGQEFSGYVSQLDHGIKAIE
                     NSLTHLSELALGGTAVGTGINTPKGYAKNVAKEIANLTGLPFITAHNKFESLAAHDAI
                     VEAHGALKTVAVSLMKIGNDIRMLSSGPRCGIGEIFIPDNEPGSSIMPGKVNPTQCEA
                     MTMVAAQVLGNDVAINIGGSNGHFELNVFKPVMIYNFLHSARLIGDVCVSFNDKCAVG
                     IAPLHDNIKKNLENSLMLVTALNTKIGYYKSAEIAQTAHKNNATLKETAIKLGYVTAK
                     QFDEWVKPEDMIGGLK"
     gene            complement(5507..6289)
                     /locus_tag="PLL59_07645"
     CDS             complement(5507..6289)
                     /locus_tag="PLL59_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMY23719.1"
                     /translation="MEFKNLLWQIDENILFITINRPDKLNALNGDTLVEIKEAFLAAK
                     NITTLKGIIITGAGEKAFVAGADIAEFQGLDANQAKALAQRGHDIFFLIEQMPIPVIA
                     VVNGFALGGGCELSMACHLRIATSNAKFGQPEVNLGIIAGYGGTQRLIQYIGKAKALE
                     LHLTADMIDAQTALNLGLLNYIENDKSAAIDKAKSIINKIATKGPIAIAKVIACTNAF
                     YTDGVDGFATEVNAFSDIFSSSDVLEGVSAFLEKRKPAFTGK"
     gene            complement(6293..7678)
                     /locus_tag="PLL59_07650"
     CDS             complement(6293..7678)
                     /locus_tag="PLL59_07650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HMY23720.1"
                     /translation="MKELLLLSGLGILSLVAEILNARKLIYPLVILGLLMNIGLCIHD
                     FGRSESIYGMMVLDNIALAFTILFSFIAVLWFLMAKNFFKDDTNLSDHFSLVLFAIVG
                     VFILTSYTHMVMLFLGVEILSIPIYVLAGSDKRNIHSNEAAFKYFLLGAFASAFLLLG
                     ITFVYGALGSFNIVQIASGAMSTYGLSLPLLIVGILLILFAMAFKMSAVPFHFWAPDV
                     YTGAPTIVTAFMSTIVKIGAVVGFYRLFSIVLTFYPQYVRLLLIIAVMSILIGNIIAA
                     SQSNIKRMLAYSGIGHVGFVVLGITMLSASTPYVTWYYLAAYTLAGLAAFWVLIIIAD
                     KDNNVDISAFNGLVKRNPLLAGTMTIALLSMAGIPPLAGFFAKYFILINVIDKGFMLI
                     AIIAILASLIGVYYYFRIIIAMFTHTTQTDNVAKKIDIQFMSKLGLGIISLLLLLLGI
                     MPDYIFSFIFR"
     gene            complement(7786..9258)
                     /locus_tag="PLL59_07655"
     CDS             complement(7786..9258)
                     /locus_tag="PLL59_07655"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HMY23721.1"
                     /translation="MLLLLLLILPVFFAVCVMFNTEKQAKNTALFGAIATLALSVSLF
                     FQYQASPLSPSFTYNAYWISALGATFYVKLDGINILLVLLTTLLIPFIIQSAAGKVIK
                     SPKAYFVLILFMQSALLGVFLAKDALLFYTFWELALIPIYLIALIWGGKGRKAITFKF
                     FVFTLFGSLFMLLAIIYMWLHNPARTFDIYDLYTVSTSLSPQQQLFVFIAFYLAFAIK
                     IPIFPLHTWQADTYQNAPTQGTMLLSGIMLKMGTFALIVWLIPMVPKAWTLASKYVII
                     LSVISVVYGSMLALAQQNFKRLLAYSSLGHVGLIAAGIFSWNLQGVQGALYQMVSHGI
                     NVVALFYICDIISSRMQTDTMQQLGGIRNVSRVFSVLFLLVVLGSIALPLTNGFVGEF
                     LLIHGIFQYNTIAAVVAGLTIILGAWYMLRAYQWMMHGDTNTLTESFGDITINEKIVL
                     SCFAVLIIFFGIFPNHLLHVSEADVKELIEQVRRASVIVQ"
     gene            complement(9274..9522)
                     /locus_tag="PLL59_07660"
     CDS             complement(9274..9522)
                     /locus_tag="PLL59_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23722.1"
                     /translation="MILVLLLTMLVVAIPTLYVFNARSVQRIALFIGATQVVVLALSG
                     KIDESLSDYIVAASNHLISASYAVMLDKMVCLLNMFSI"
     gene            complement(9526..11427)
                     /gene="nuoL"
                     /locus_tag="PLL59_07665"
     CDS             complement(9526..11427)
                     /gene="nuoL"
                     /locus_tag="PLL59_07665"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HMY23723.1"
                     /translation="MEHLVYLIPLFPLLSFAIIALLGKHLSKNITSYLAPGSILASFI
                     VALLIFGSQIGHNEAVSVPLFSWIHVGDFNANFSFLVDSLSIIFTLVITGVGFLIHVY
                     STGYMHDEKDYNRYFSYLNLFVFFMLLLVLGDNLLLMFVGWEGVGLCSYLLIGFWFKN
                     INYASAAKKAFVMNRIGDLGFLLGIILIFGVFGSVNYKEVFTLAGNVVIDGQLITWIT
                     VLLFIGAIGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIARCGVLFSMSA
                     FTMQLIAIIGIATAFLAATIALFQNDIKKVLAYSTVSQLGLIFCALGVGAFSAGVFHV
                     VTHAFFKALLFLGAGSVIHGMSGDQDIRNMGGLKNKMPITYITFLIATIAISGIPPFA
                     GFFSKDQMLAEIFVHNKILFALALIVSLMTAFYMFRLLFLTFFGEFRGNDIVKNHIHE
                     SPKSMTIPLMILAVLSVLGGFIGFPHELGHAIGIHHWLDGFLATAVKMQEVEISLNTE
                     LLLMAITIVLILIVILIAKNSFLSKKIVPHSDEDAMPLWKEIIYYKYYIDEIYEAVIV
                     RPLTRLAGMLYQFVERSGIDAIVNGIGTSVVNWSQLLRLTQTGGLGFYVITMVISMVV
                     LFILMFIKL"
     gene            complement(11430..12311)
                     /locus_tag="PLL59_07670"
     CDS             complement(11430..12311)
                     /locus_tag="PLL59_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY23724.1"
                     /translation="MKKFGLYIVVVLALSSCVTKKKFQATQTEKDKIEMALRQTTSDL
                     AECEKKFGKKTSDFDALSDELAKAKTKIEELIKEVDYQKSANGKLLDQLSALSVISKE
                     GAASIKQSLETIDKQRGEISVLNSQIRSKDSLNLVLVMNLKRSLSDVNDTDVNVKVDK
                     GVVLISLSDNMLYKSGSSEISSKAGAVLEKIAKVINDYKTFDVLIEGNTDNVPIATSC
                     LLDNWDLSVKRATSIARALQKSYGVDPSRIIAGGRAEYNTKVPNDTKANKAINRRTEI
                     IIMPKLDEFFKLLEIKN"
     gene            complement(12342..12662)
                     /gene="nuoK"
                     /locus_tag="PLL59_07675"
     CDS             complement(12342..12662)
                     /gene="nuoK"
                     /locus_tag="PLL59_07675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HMY23725.1"
                     /translation="MKEVLIGIPIQWYIYLSVTLFCIGIFGVLFRRNAIIMFMCIELM
                     LNAVNLLMVAFSRYLGDTGGQVFVFFVMVVAAAEVAVGLAILMMIYRNTKSTDIDTLN
                     RLKW"
     gene            complement(12714..13217)
                     /locus_tag="PLL59_07680"
     CDS             complement(12714..13217)
                     /locus_tag="PLL59_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMY23726.1"
                     /translation="MPLSQYLFFFLSFVAVLSALLVILDKNPVHSVLYLIITFFSIAG
                     HFLLLNANFLFIVQIIVYTGAIMVLFLYVIMMLNLNKEIEPHKKNIPKVAAAISAGIF
                     LMSLIALLKGADVNLLPAARNSNIGLLENLGNVLFKEYMLPFEISSLLFLGAMVGAVF
                     LSKKEIN"
     gene            complement(13257..13868)
                     /locus_tag="PLL59_07685"
     CDS             complement(13257..13868)
                     /locus_tag="PLL59_07685"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HMY23727.1"
                     /translation="MQLTNRKKVVVKKEMSLAEKLYLPAITNGMVITLKHFFKKSATV
                     KYPEVKRELSGTYRGQHVLKRDEQGAERCTACGLCAVACPAEAITMEAGERKKGEENL
                     YREEKYAVIYEINMLRCIFCGLCEEACPKEAIFLTDRIVPTDLKRDEFIYGKDKLVED
                     IKQPVDVTKRQTQNVLDFKKDKTKGHNQTWTDKQKLLSDKKHH"
     gene            complement(13885..14919)
                     /gene="nuoH"
                     /locus_tag="PLL59_07690"
     CDS             complement(13885..14919)
                     /gene="nuoH"
                     /locus_tag="PLL59_07690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HMY23728.1"
                     /translation="MIVFIIYKAVLVLIVFLISLGVAAYSTYGERKVAAFMQDRVGPD
                     RAGPFGMLQPLADGLKMFMKEEIIPSVSNKFLFVLGPSLAMLTALMTGVVIPWGSSIT
                     IGNYTFPLQITDINIGVLYVFSVVSFGVYGIMIGGWASNNKYSLLGALRASSQMISYE
                     IAMGLAIVALVMTTGTLSLGEIVAQQSGTWFNFVYQPLGCLIFIICAFAETNRAPFDL
                     PECETELVGGYHTEYSSMKLGFYLFAEYINMFISSAIIVSLYFGGYNLPFQNLITQYI
                     DGNVLSILQFLCFFTKVLMGIFFFMWVRWTIPRFRYDQLMNLGWKILIPLAIFNILAT
                     GVMMYALGKV"
BASE COUNT         4918 a         2761 c         2396 g         4917 t
ORIGIN      
        1 ctattgctgg taagaagctg acttcttttt tccaaaactc cttcatgagt acttgtacta
       61 aaagtactga tgttcgagat tgataatcta aatacactgt cgaaatatct tctaatgcaa
      121 catctgagaa taagcaaact gtttttacag ctccattagc accgatacaa aatggcgaaa
      181 ctatgcgtgg gtttttcaat tgcggaataa ccgcaattgg tactaatcca atgtctatat
      241 cgccacttaa caatttctgc gcacattgtg ctggcgtttc ttgtgatagc tttatattat
      301 catatataga tggatgatgc agtccatgta taaatggctt ggtattgaga tatgatacta
      361 cagaaatttt atagcttgga ttcattaaac agtattttat tgcgatgtat cattttaaac
      421 gtttaaatgc gtagtatcat tttcctttat caagctaaat gtattgtcgt cataacttaa
      481 gtgatataaa caagtatttt gatggtcaaa attatccatt tgcgcaatat ctattcttaa
      541 taataaacaa attaaaatgc gtaaggttct accatgtgta caaactaaaa ttgtttgtgc
      601 tgtagtattc tttatttctt caataaatgg caataatctt tcagccaaat cattagcact
      661 ttcaccacct tcaattttca cggaaaggtt tccatttttc cattcttcaa taatatcgta
      721 atattctttt tgtaaaccag cgtgatgctc aacgccttca aatattcccc aatcaatctc
      781 atctaagttt gtgcgtataa gatgtggtat tttttttgaa ataaatgaat caaccgtttg
      841 ccaagttcgt ttgagcgatg atgtataaac gatatcaaat ttaatttctt tatgcgcttc
      901 aaaaaaaagc tttgcttgtt gtattccagt gtcgtttata tctgagttaa tgcctctgcc
      961 ttgaacgatt tgctttacat taaaatctgt ttgaccatgg cgtataatat agatttcttt
     1021 ctttgtgttt gatgtttgca ctaatcttta ttttggatgc aaatatactt attataaaga
     1081 tgttcttcta tttgaaaaaa acgaaaatgt caaacccgaa ttagatggag ataaaaaatg
     1141 gactttttaa aaagcttcaa ttttattcaa tgtatgtcaa aatacaattg acaattagcc
     1201 aaccatcgta tttactggca acttagataa ttctaaatgg ctgttcattt tttggatttt
     1261 ttgaaggatt tcgcttgcta cttctatact ttttaaacta tccaatgccg taacattagg
     1321 ttctttatta aacaacaaac attctgcaaa actattcaac tcttctttca gtgcatcata
     1381 ctcttctgtt accattgttt ccgtgttgat atagcgcgtt gtttcctgta tgtgcattgg
     1441 aatagcattg tgttttgctg tttcggataa gtgcaacaat tctgcttctc tatttaaaaa
     1501 atctaatgta atatggctgt ctttttgaaa gaaaagcatt ttgcgcattt tttgaacaga
     1561 agttctgctt gctgttaaat tcgctacaca tgcgttatca aattcaatac gagcgttggc
     1621 aatatcaatc tgatcagata atacggaaac gccgctggca ctaatgcttt taatattagc
     1681 ttttacgata gataaaataa tatccaagtc gtgtatcatt acatccatga tgatggaatc
     1741 gttggtgcgt ttagcatcaa ataatgacaa acggtgcgac tcaataaata aaggctgtat
     1801 cgattgttgt ttcagtgcta aaaatacagg attatatcgt tccgtatgtc caacttgaaa
     1861 gatgatgcct gcctcacgaa tcaattctac caactttttt gcctcatctg ccgaattgga
     1921 tatcggttta tctacatata catgcttgcc aaatctaatg gctttttcgg cataataata
     1981 atgggaatct gatggcgtta agatttcaac agcatcgcat ttatttatca attcatctac
     2041 atcatcaaaa caatccacgc catattcttc ctgaataggt ttaagtttat gctgctcttt
     2101 gtcgaagcaa cctattactt taaaatatgg agatatcaat aagcgttcaa gatgctgtac
     2161 acctaatcgc ccgataccga tgatgccaat ttttatcatt tctatacaat atttacttgt
     2221 atataatatc agtagtatat cgtttattac agcccatctc aatacatcgc actgcaagct
     2281 tagcagcaat catttgttta aaaagattgt ttcgcagctc gcaatgtcgt taataatcga
     2341 aaatttagct tgctatatga catgatatat cctttctata gtataaaatt aaatttctat
     2401 ttgattgtta attaataaat taatccacga gatgtgaatt aagccactta tacacctgaa
     2461 attcattaaa aatgattaat aatttcttta tgctagattc cgtatttttg tatggtgatt
     2521 agcgataaaa aaatagcatt tcatacttta ggttgcaaac tcaatttctc cgaaacttcg
     2581 gcaattacac gacaaatgca agcgcgtggt ttttcgactg tagattttga ggaagtttct
     2641 gatgtttatg tcatcaacac ttgttctgtg acggaacaag ctgacacaaa atgcagaaat
     2701 attgtgcgca aagcattaaa attaaatcca gatgcgtatg tcgtggtaat tggttgctat
     2761 gcgcaattaa aacctaaaga aatcgcatct atcgaaggtg ttgatttggt gttaggtgca
     2821 gccgaaaaat ttaagttgcc agaaatcatt acagacctta ctaaaaatcc atgtggacaa
     2881 gtattggcct ctgaaatttc gaatgctaat ttttttgttg acgcctattc tgttggtgat
     2941 cgtacacgct cttttttaaa aatccaagat ggctgcgact acaaatgctc cttttgtacg
     3001 attccattgg cacgtggaaa aagtcgaagt gacacgccag aaaacatttt gaaaaacgcc
     3061 aaagatttag ctgctaaagg tgtgaaagaa atcgtattaa caggcgtcaa tacaggtgac
     3121 tatggcaaag gcattgcatc agaaagtaat ttttttgaca tcgtaaaaat gttagatgaa
     3181 gtagaaggta tagagcgata tcgaatctct tctatcgaac ctaatttatt aacagatgaa
     3241 attattgagt ttgttgcaaa cagcaaacgt tttatgccgc actttcacat tccattgcaa
     3301 agcggaagtg ataagatgct aaaattaatg caacgtagat acaaacgcga tttatataaa
     3361 agcagagttg aaaaaattaa atctgtgatg ccacatgctt gtattggcgt agatgttatt
     3421 gttggttttc cttccgaaac tgaagatgat ttcttggaaa cttatcaatt catcaatgca
     3481 ctcgatattt cgtatttaca tgtattcacc tattctgaac gtcagaatac aaaagcctta
     3541 gaaatagaag gcatcgtgcc gatgcctatc cgcaatgaga gaaatgccat gttgcgttct
     3601 ttaagtgaaa aaaagaaacg atatttctac caacaatttt taggacaagc aaaaacggtg
     3661 ttgttggaac aagagcaaaa aggcaatatc atgcatggtt ttacggacaa ttatatcaaa
     3721 atatcagttc catatcatgc tgcatctgtc aatcaaatgg ctactgctca attacaatct
     3781 atcgacgacg ctggtgatgt gttggctata ttgatagatt gatttatact tattcgattt
     3841 tacaattctt gacatttatc tttctatact gaattttgta taacaatagt gtttcattat
     3901 tatggctcaa aaccattgtc attattgact caatagacta ttttttgctc catttttgac
     3961 cacattattc tagaaaaaat aagattgcct tttgcaggga aatgacttta taaaaaaaac
     4021 acgatgatat attgctatat catcgtgttt tataaaatac ttatttaata gcttatttca
     4081 gtccgccaat catgtcttct ggttttaccc attcgtcgaa ttgtttagcg gtaacatagc
     4141 ctaatttaat agctgtttct ttaagcgtag cattgttttt atgcgccgtt tgtgctattt
     4201 cagctgattt atagtagcct atttttgtat ttaatgcagt cactaacatt aaagaatttt
     4261 ctaaattctt tttaatatta tcgtgcaaag gtgcaatgcc taccgcacat ttatcattga
     4321 atgacacaca tacatcgcca attaagcgag cactatgcaa gaagttgtaa atcatcacag
     4381 gtttaaacac atttaattca aaatgaccat tgctaccacc aatattgatt gcaacgtcat
     4441 tgcctaatac ttgtgcagcc accatagtca tggcttcgca ttgtgtagga ttaaccttgc
     4501 ctggcataat agaagagcct ggttcattgt caggaataaa tatttcgccg ataccacagc
     4561 gtggtccaga agataacatt cgaatatcat ttccgatttt catcaatgac acagccactg
     4621 ttttcaaggc gccatgtgct tccacaatcg catcatgcgc ggctaaagat tcaaatttat
     4681 tgtgtgctgt aataaaaggt aaacctgtta agttggcaat ttcttttgct acatttttag
     4741 catatccttt tggtgtgtta atgccggtgc ctactgctgt tccgccgagc gccaactcag
     4801 aaagatgtgt taaagaattt tcaatagctt taatgccgtg atctaactga gaaacataac
     4861 ctgaaaactc ttggcctaat gttaatggtg ttgcatccat aaaatgtgta cgaccaattt
     4921 ttacgacttt agcaaattct ttcgatttct tagctaatgt atttctcagc tttttaattc
     4981 ctggaattgt tttttccatt aaaatagtat aagctgcgat atgcatggct gtaggaaatg
     5041 tatcgtttga tgattgtgat ttattgacat catcatttgg attgattagc tttttttcat
     5101 cagtcaactg accacctaaa agcacatgtc cgcgataggc aattacttca ttaacattca
     5161 tatttgattg tgtgcctgaa cccgtttgcc aaaccactaa tggaaactga tcatctagct
     5221 tacctgctaa aatttcgtcg caaacttttc cgatagtatc gcatttttgt ttgtctaata
     5281 ctttcgcttt gtagttggta attgctgctg cttttttcaa atgagcaaat gcgcgaataa
     5341 tctctttcgg cattttattg atgtcttgcg cgatttgaaa attctcgata gagcgttgcg
     5401 tttgtgcacc atagtaagcg tcgataggta cgtttacttt tcccatggtg tctttttcaa
     5461 ttctgtattg catttttttg agtattgaat gttgagtatt ttttaattat tttccagtga
     5521 atgctggttt acgcttttct aagaaagcac ttacaccttc aagcacatct gaagaagaaa
     5581 aaatatcgct gaatgcattt acttctgttg caaaaccatc gacgccatct gtataaaatg
     5641 cattcgtgca tgctattact tttgcgattg caattggtcc tttggtagca attttattta
     5701 taatgctttt tgctttatcg atagctgctg atttgtcatt ttctatgtaa ttcaacaagc
     5761 ctaaattcag tgctgtttgt gcatctatca tatcagcagt aaggtgtaat tctaaagctt
     5821 ttgctttacc gatatattgt attaatcttt gtgtgccacc ataacctgcg ataattccta
     5881 ggttgacttc tggctgacca aattttgcgt tgcttgttgc aatgcgtaaa tgacaagcca
     5941 tagacaactc acagcctcca cctaaagcaa atccattgac tactgcaatt acaggaattg
     6001 gcatctgttc tattaaaaag aaaatatcgt gaccgcgttg tgccaatgct tttgcttgat
     6061 tagcatctag accttgaaac tcagcaatat cagcaccagc aacgaatgcc ttttcgccgg
     6121 cgcctgtaat aataattcct tttagtgttg taatattttt tgcagcaaga aatgcttctt
     6181 tgatttctac taaagtatcg ccgtttaatg cgtttagttt atctggtcta ttgattgtga
     6241 tgaacaatat gttttcatca atttgccaaa gtaagttttt gaattccata aattatctaa
     6301 atataaaact gaatatataa tctggcataa tgccaagtag taataataaa agcgagataa
     6361 tacctaagcc caatttactc ataaattgaa tgtcgatttt tttggcaaca ttgtcagttt
     6421 gtgttgtatg cgtaaacatg gcaattataa ttctgaaata ataatacaca ccaattaaag
     6481 atgccaaaat tgctatgata gctatcaaca taaagccttt atcaatcaca tttattagaa
     6541 taaaatattt agcaaaaaag ccagccaagg gtggaatacc agccatcgac aacaatgcaa
     6601 ttgtcatggt gcctgccagt aaaggattgc gttttactaa tccattgaat gcggaaatat
     6661 cgacattatt atctttatcg gcaatgataa ttaaaaccca aaatgctgct aatcctgcta
     6721 aagtataggc tgctaaataa taccaagtaa catatggcgt tgatgccgac aacatcgtga
     6781 tacccaatac tacaaaaccc acatgaccaa taccagaata agccaacatg cgttttatat
     6841 tgctttgtga tgctgctatt atattgccaa ttaaaataga cattactgca ataatcaaca
     6901 acaatcgaac gtattgcgga tagaaagtaa gtacgatact gaacaggcga taaaaaccga
     6961 ctactgctcc gatttttacg atggtggaca taaatgctgt aacgatggtt ggtgcacctg
     7021 tatatacatc gggtgcccaa aaatggaagg gtactgccga cattttaaag gccattgcaa
     7081 ataaaataag taagatacca actattaata atggcaaact taatccatac gtggacatag
     7141 caccagatgc aatttgaacg atattaaaac tgcctaatgc accatataca aatgtgattc
     7201 ctaatagtaa aaatgcactt gcaaatgcac ctaataagaa atatttgaat gcagcttcat
     7261 tcgagtgtat attgcgttta tcgctacctg ctaaaacata tattggaatg gataaaattt
     7321 caacaccaag aaacaacatg accatgtgtg tataggaagt taaaataaaa acgccaacaa
     7381 tagcgaatag cactaaggaa aaatgatctg acagattggt atcgtcttta aagaaatttt
     7441 ttgccatcaa aaaccataaa acagcaataa aagaaaataa aatggtgaat gctaatgcta
     7501 tattgtctaa caccatcatg ccataaatac tttcgctacg gccaaaatcg tgtatgcaca
     7561 atccaatgtt catcaacaaa cctaaaatca ctaatggata gattaacttt cgcgcattta
     7621 agatttctgc cactaatgac aatatgccta atcctgataa gagtaataat tctttcatct
     7681 atttatcctg tttttggtgt attatttgaa tttgattttc tttttttaaa acatcaatat
     7741 ttcaatttcc tacacctatt tttaagactt ttacgtccat tttttttatt gtacaatcac
     7801 cgatgctctc ctcacctgtt caattaattc ttttacatca gcttctgaaa catgaagtaa
     7861 atgatttggg aaaattccaa agaaaataat taaaacggca aagcaactca atactatttt
     7921 ttcatttata gtgatatcgc caaagctttc tgttagtgta tttgtatcac catgcatcat
     7981 ccattgataa gcacgtaaca tataccacgc ccctaagatt atggttaatc cagctactac
     8041 agcagctatt gtattgtatt ggaatatacc atgaattaat aaaaattcgc caacaaaacc
     8101 attggttaat ggcaaggcaa tactgcctaa cactactaat aaaaacaaaa cactaaacac
     8161 acgtgataca tttctgatgc cacctaattg ttgcatcgtg tctgtttgca tacgtgaaga
     8221 aatgatatcg catatataaa acaaggcaac gacatttatg ccatgtgata ccatttgata
     8281 taatgcacct tgaacacctt gcaagttcca agaaaaaatt ccggccgcaa tcaaaccaac
     8341 atgtcctaaa gatgaatagg ctaataatct tttgaaattt tgttgtgcta aagctaacat
     8401 ggaaccatat actacgctta ttacacttaa tattatcaca tatttcgatg ctaatgtcca
     8461 agctttagga accattggaa tcaaccacac gattagtgcg aatgtaccca ttttaagcat
     8521 gataccagac agcagcatgg tgccttgtgt tggtgcattt tggtaagtat ctgcctgcca
     8581 agtatgcaaa ggaaaaatag gtattttgat agcgaatgct aagtaaaatg caataaagac
     8641 aaacaattgt tgctgtggac tcaagctagt acttactgta tataaatcat aaatatcaaa
     8701 tgttcttgct ggattgtgga gccacatata gataattgct aatagcataa acaagcttcc
     8761 aaataaggta aaaacaaaaa acttaaatgt aattgctttt ctgcctttgc caccccaaat
     8821 aagcgcaatc aaatatattg gtatcaatgc caattcccag aatgtataaa acaataatgc
     8881 atcttttgct aagaaaacac caagcaatgc actctgcata aaaagtatta acacaaaata
     8941 tgctttagga cttttaatta ctttgccggc agcactttgt atgataaatg gaatcagcaa
     9001 agtagtgagt aacaccaaga gtatattgat gccatctaac tttacataaa aagtagcacc
     9061 taatgcgctt atccaataag cattatatgt aaaacttggt gataatggcg atgcttggta
     9121 ttgaaaaaac aaagacacac ttagcgctaa agtagcaata gcgccaaata gtgctgtatt
     9181 tttcgcttgt ttttctgtgt taaacatgac acagactgca aaaaaaacgg gtaatatgag
     9241 caataaaagt aataacatgt atataattaa tttctaaatg gaaaacatat tcaacaagca
     9301 caccatctta tcaagcataa cagcatacga tgccgaaatg aggtgattac tagcagcaac
     9361 gatatagtca gacaagcttt catctatctt gccggaaagt gctaatacaa caacttgtgt
     9421 ggcaccaata aacaaggcaa tacgttgcac agatcttgca ttaaaaacgt ataaagtagg
     9481 aatagctact actaacatgg tcaacaataa aacaaggatc ataacttata attttataaa
     9541 cattaaaata aataaaacaa ccatacttat taccatagta atgacataaa aacctaagcc
     9601 acctgtctgt gtcaatctta ataattgact ccaattaaca acagatgttc cgattccatt
     9661 tactattgca tcgatgcctg agcgttccac aaactgatac aacatacctg ctaatcgtgt
     9721 caatggtctt actattacgg cttcataaat ctcgtcgata taatatttat aatagataat
     9781 ttctttccat aatggcattg catcttcatc actatgcggt acaattttct tactcaagaa
     9841 actatttttt gcaataagta ttacaataag aatcaacaca attgtaatgg ccattaatag
     9901 taattctgtg tttaaagaaa tttctacttc ttgcattttc acagctgttg ctaaaaagcc
     9961 atctagccaa tgatgtatgc caatggcatg tccaagttcg tgcggaaatc ctataaagcc
    10021 acctagtacg gaaagcactg ccaatatcat taatggaatt gtcatgcttt taggtgattc
    10081 gtgtatatga ttttttacga tatcgttacc acggaattcg ccaaagaaag taaggaaaag
    10141 caaacggaac atataaaatg ctgtcatcaa tgaaacaata agagctaatg caaacaagat
    10201 tttattatgt acaaaaattt ctgctagcat ttggtctttt gagaagaaac ctgcgaatgg
    10261 cggaatacca gaaatagcaa ttgttgctat taaaaatgtg atatacgtaa ttggcatttt
    10321 gttttttaag ccacccatat tgcgaatatc ctgatcacca ctcatgccat gaataacact
    10381 gccagcacct aaaaacaata aggctttgaa aaatgcgtgt gtcaccacat ggaacacacc
    10441 tgcagaaaat gcgccaacac ctaatgcaca aaatatcaaa cctaattgcg atacagtaga
    10501 ataggccaat acttttttaa tatcattttg aaaaagtgcg attgttgcag caaggaatgc
    10561 agtggcaata cctattattg ctattagttg catcgtaaat gccgacatag aaaatagcac
    10621 gccacatctt gcaatcatat aaataccagc ggttaccata gtagcagcat gtattaacgc
    10681 ggacactggt gttggtccag ccattgcatc tggaagccaa gtgtataatg gtatttgtgc
    10741 cgactttccg attgcaccaa taaacagtaa tacggtaatc caagttatta attgaccatc
    10801 gataacaaca ttgccagcta gtgtgaatac ttccttataa ttaacacttc cgaatacgcc
    10861 aaatattaag atgataccta ataaaaagcc taagtcgcca atacgattca tcacaaatgc
    10921 ttttttagca gcacttgcat aattgatatt tttgaaccaa aatccgatta ataaataaga
    10981 acacaatccg acaccttccc aaccgacgaa catgagcaat aagttatcac caagcactaa
    11041 caacaacatg aaaaacacaa ataaattcaa ataagaaaag tatctgttat aatctttctc
    11101 gtcgtgcatg tagcctgtcg aatatacgtg tatcaaaaaa cctacacctg taattacgag
    11161 tgtgaaaata atagataatg aatcgactaa aaatgaaaaa ttcgcattaa aatcgccaac
    11221 atgaatccat gaaaataaag gaacgcttac agcttcatta tgaccgattt gacttccaaa
    11281 aattagcaaa gcaactataa aagatgccaa tatgcttcca ggtgccaaat agcttgttat
    11341 atttttagac aaatgtttac caagcaaagc gataatggca aagcttagca aaggaaataa
    11401 tggtatgaga tatactaaat gttccactct taatttttaa tttctagtaa tttaaaaaac
    11461 tcatctaatt taggcataat gattatttca gtacgtctat ttattgcttt gtttgctttt
    11521 gtgtcattag gcacttttgt gttatactca gcgcgaccgc ctgcgatgat tctggaagga
    11581 tctacgccat agcttttttg taaggcacga gcgattgatg tagctcgttt cacacttaaa
    11641 tcccaattat ctaataagca actggtagca ataggtacat tatctgtgtt gccttcaatc
    11701 aacacatcaa atgttttata atcattgatt actttagcaa ttttctctaa tacagcacct
    11761 gctttagatg aaatttctga gcttccagat ttatacaaca tattatcaga caaagaaatt
    11821 aagacaacac ctttatctac ttttacattt acgtcagtgt cattaacatc agataatgag
    11881 cgtttcaaat tcatgactaa cactagattc aatgaatctt tagagcgaat ttgtgaattg
    11941 agcacagaaa tttcgccacg ttgtttgtct attgtttcta atgattgttt gatacttgct
    12001 gcaccttctt tgctaataac ggaaagcgca gataattgat ctaatagttt tccatttgca
    12061 gatttttgat aatcgacttc ttttatgagc tcttcaattt tagtttttgc ttttgctaat
    12121 tcatcagaaa gcgcatcgaa gtcacttgtt tttttaccaa attttttctc acactctgcc
    12181 aagtcagatg ttgtttggcg caatgccatt tcaattttat ctttttccgt ttgtgttgct
    12241 tgaaatttct ttttggtcac acaagaagaa agtgccaata caaccacaat atataatcca
    12301 aactttttca tatctttttt attagattaa tgacaaataa tttaccattt taatctgttt
    12361 aacgtatcga tgtctgttga ttttgtattt ctataaatca tcattaatat agccaatccg
    12421 acagcaactt ctgctgccgc tacgaccatt acgaagaaga caaaaacttg gccaccagta
    12481 tcgcctaaat aacgcgagaa agccaccatt aataagttta ctgcattcaa cattagttcg
    12541 atacacataa acatgataat ggcatttctt cgaaataaaa caccgaatat tccgatacaa
    12601 aacaaggtaa cacttagata aatgtaccac tgtattggaa tgcctattaa tacttctttc
    12661 atattttttt cgtttttaca tcttgctttg tagcaagcgc aaattttatt tatttaattt
    12721 atttcttttt tgcttaaaaa tactgcgccc accattgcgc ctaaaaacag caatgatgaa
    12781 atctcaaatg gtagcatata ttctttaaac aatacatttc ctaagttttc taaaagtccg
    12841 atattactat ttcttgcagc tggcaacaga ttaacatcgg cgccttttag caaagcaatt
    12901 aagctcatta aaaatatgcc agcagaaatt gctgctgcca ctttaggtat atttttttta
    12961 tgtggttcga tttctttatt taagttgagc atcatgatga catataaaaa caataccatg
    13021 atagcacctg tatagactat tatttgtacg atgaataaga aatttgcatt taatagtaag
    13081 aagtgaccag cgatagaaaa aaaagtgatg attaaataca atacggaatg tactggattt
    13141 ttatccaaaa taaccaacaa tgcgctaagc acagctacaa aggacaaaaa gaaaaataag
    13201 tattgtgata atggcatatt gactaaataa tttattcgtt ataactttta tattgcttaa
    13261 tgatgttttt tatcgctcaa taatttttgt ttatcagtcc aagtttgatt atgacctttt
    13321 gttttatctt tcttaaaatc taatacattt tgagtttggc gttttgttac atctactggt
    13381 tgtttaatat cttcaactaa tttatctttt ccataaataa actcatcgcg tttcaaatct
    13441 gtaggcacaa ttctatctgt taaaaatatc gcttcttttg ggcaagcttc ttcgcacaaa
    13501 ccacagaata tgcaacgcaa catattgatt tcgtagatga ctgcgtattt ctcttcgcga
    13561 tacaaattct cttctccttt tttacgctct ccagcttcca tggtgatggc ttctgccggg
    13621 caagccactg cgcataaacc acatgcagta caacgttctg cgccttgttc atcgcgtttc
    13681 aatacgtgct gtccgcgata ggtaccagaa agttcgcgtt ttacttcagg atatttaacg
    13741 gtagccgatt ttttgaaaaa atgttttaag gtaatcacca taccattagt aatagctggc
    13801 agatataatt tctctgctaa gctcatttcc ttttttacga ctactttttt tctatttgtt
    13861 aattgcatta ttcttttttt acaattatac tttacccaat gcatacatca tcacacctgt
    13921 tgccagtatg ttaaagatgg ctaatggaat taatattttc caacctaaat tcattaattg
    13981 gtcatatcta aatcttggta ttgtccaacg aacccacata aagaaaaaga tacccattaa
    14041 tacttttgtg aagaaacata agaattgcag aattgataaa acattaccat caatatattg
    14101 tgtaatcaaa ttttggaaag gcagattata accaccaaaa tataatgaca caattatcgc
    14161 tgaagagata aacatattga tatactctgc aaataaataa aatccgagtt tcatcgatga
    14221 atactcagta tgataaccgc cgacaagctc tgtttcgcac tcaggcaagt cgaaaggtgc
    14281 tcgatttgtt tcggcaaaag cgcaaataat aaatattaga cagccgagtg gttgataaac
    14341 aaaattaaac catgttccgc tttgttgtgc tactatctcg cctaagctta aagtacctgt
    14401 tgtcattacc aatgcaacaa tagccaaacc cattgcaatt tcgtagctta tcatctgaga
    14461 ggaagcgcgt aatgcaccta ataatgaata tttattgtta gatgcccaac cgccaatcat
    14521 gatgccgtaa acgccaaaag atactacgct aaatacatat aaaacgccga tattgatatc
    14581 ggtaatctgt agcgggaacg tgtaattgcc gatagtaata ctgctgcccc aaggtattac
    14641 tacccccgtc attagagcgg ttagcatagc taagctcggg cctaaaacga aaagaaattt
    14701 attagataca gaaggaatga tttcttcttt cataaacatt ttcaatccat cagctaaggg
    14761 ttgtaacatt ccaaaagggc cggctctgtc ggggccaacg cggtcttgca taaatgcagc
    14821 gactttacgc tctccgtaag ttgaataggc tgctacgcct aatgaaatca agaacactat
    14881 tagtacaagt acagccttat aaattataaa tactatcatt tcttttctgt attgtttggg
    14941 ttcaatggtc cttctcctaa ttccgctttt tttccctcca attgtttaag tt
//
LOCUS       DAOQQV010000063        14964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1291_length_14964_cov_8.332014, whole genome shotgun sequence.
ACCESSION   DAOQQV010000063 DAOQQV010000000
VERSION     DAOQQV010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1291_length_14964_cov_8.332014"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1575)
                     /locus_tag="PLL59_07695"
     CDS             complement(112..1575)
                     /locus_tag="PLL59_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23729.1"
                     /translation="MLIAFANAQTDVDALRYSTPSLQGTARNIAVGNTMGTIGAEISA
                     LGTNPAGIAKFSATEFTITPAISIAKSKSTFLNTSTKDDKVKFQLSNLGIVIAGRNSN
                     KSNAGKWNGLKFGFAINRLANYNNDFTFSGYNDKNSLLNAYYEKLNDRDYITDSTDAA
                     DKYPFDASIAYRLGLITIDSLGNTFTATNNGHMQQTYNIQKTGGMDELALGIASMYKD
                     KLMLGASIGIPFISYTERIRLEEKDVNDSAYNLNSFLNASYLKTRGVGFNLKVGIIGS
                     PIPNLRLSLAIQTPSIIFMKDAFLTQMEVDYANQSVIYTGESPEGASKYKYVQPWKLT
                     TGAGYIHKYGFVSMEYELSDAGNSKFKFSDASANQYERFVNANIDNKYALFHTIKAGI
                     EFKYDPVRIRGGVQYRTSPFKKSAAPSNLSTDVLTFSAGIGYRGKHFFADLAYMQTNF
                     KEMYVPYQLNTTAWNPVPAATLSTKNPLVVVTVGYKL"
     gene            complement(1628..2686)
                     /locus_tag="PLL59_07700"
     CDS             complement(1628..2686)
                     /locus_tag="PLL59_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23730.1"
                     /translation="MKNIVVFMTLAIFILPANISLAQDDVYDNPRPKQSKVKKTYTEP
                     TEPSEKKISTEISQPYTESEQTTTRIADKNFIADNYSDNYTDYNNGYDSDWNYSYTDR
                     IRRFHNPSFQFNYGWNNWNNNYYNPWNSYNNNFGWNNWDNYSFTPSWYHSYANFYNPF
                     NSTQIIIIQPGFGYSSWYNTGYNNPWNAWNNFGWNSWNNNWYGFSPYCSNAIYNNSYY
                     HHYGINENYYNKRNVVNTPRTGGYNNTGNVQSGNGNYNQYNNYPSNTNNGKGGFNDNS
                     STTNTQQVTKTTKWNTKQSNSSTNTPNENVYKYAQPNNHSNNSWNNTPSYNNNSYERE
                     KINTNDGNSGIKIGTRSK"
     gene            2805..4286
                     /gene="proS"
                     /locus_tag="PLL59_07705"
     CDS             2805..4286
                     /gene="proS"
                     /locus_tag="PLL59_07705"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMY23731.1"
                     /translation="MSKEITSRAENYSEWYNDLVLKGGLADYSAVRGCMVIKPYGFTL
                     WENMRDVLDKMFKETGHVNAYFPLFVPKSLFEAEEKNAEGFAKECAVVTHYRLKTNPD
                     EPGKLIVDPEAKLEEELVVRPTSEAIIWNTYKKWIQSYRDLPILVNQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHATKAEAVAETIQMINVYADFAENYMALPVIKGVKSESERF
                     AGAEDTYTIEAMMQDGKALQSGTSHFLGQNFAKAFDVTYVNKDNQPEYVWATSWGVST
                     RLIGALIMAHADDDGLVMPPRIAPHQVVIIPFLSKNEEETQRMITTANELKSDLQKLG
                     IRAIVDSDETKRPGFKFAEYELKGIPIRVVIGPRDLANGMVEIARRDTKEKSSYSVEN
                     LPKSIEQLLDNIQQNLFDKAKAFRDSNITKVDDFATFQKLLDEQPGFILAHWDGTAET
                     EEKIKELTKATIRCIPLDNPQEDGKCILTGNPSKQRVLFARAY"
     gene            4330..5931
                     /locus_tag="PLL59_07710"
     CDS             4330..5931
                     /locus_tag="PLL59_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HMY23732.1"
                     /translation="MIKNLILELPPEVAYDEIIFHQYIKNYLQINDAQEYHIRISKRS
                     IDARSRNIKVNINATVFIDEQPDSIVSPIQYKNVANAKSVIIVGAGPAGLFAALRLIE
                     LGVKPIVIERGKDVQDRRRDLANINKKNIVNENSNYCFGEGGAGTYSDGKLYTRSKKR
                     GDINRILEIMVTHGAQENILFEAHPHIGTNKLPKLIQAIRNTILEYGGEIHFNTKVVD
                     FIVNKNEMKGVVAVRTDTLQTLSTTPNIEYIGIGVILATGHSARDIFELLHQKNILIE
                     SKPFALGVRIEHQQSLINQIQYHGNDCTILPAATYSLVEQVKVGTTQKAVFSFCMCPG
                     GFIVPSATHQEEIVVNGMSPSKRDSPYSNSGMVVAINELDWKDFNHLGPLAALAYQKN
                     IEQKAWQIAGRTQAAPAHRMLDFVKNKPSDDLLDTSYQPGLVSVDMHDILPSTIAYSL
                     QEGFTSFGKKMKGYLSNDAQIIGVESRTSSPIKIPRDKESLEHITIKRLFPCAEGAGY
                     AGGIMSAAIDGERCAEKLVALYASL"
     gene            6110..7534
                     /locus_tag="PLL59_07715"
     CDS             6110..7534
                     /locus_tag="PLL59_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HMY23733.1"
                     /translation="MKRLLFLVAITAFLFSCKKESAERQRNQIANDPTIIQDQYGRQL
                     ILHGLNTASNAKSDSLRNPWISESDVEREVNIFGFNVVRYLIFWDAIEPQKDFFDDAY
                     LDRVEERVNWYSSRGMYVMLDMHQDMYSIIFGGDGAPAWACRTNGAAPIELPGGTPWW
                     LKNIDPQVVNSWINFWAYTNHKDLQDHYILAWKKVIERFKNNPYVIGYDLMNEPWGGD
                     LIKVFITGEFERYQLTAFYNRLIPALRAADPDKYMFFEPTPAPVTFGMPSNLRKISDT
                     RSSSRTVYAPHCYPFDTHEGTGYTAASKSQLIDFERERKKESQLHGNVPVLIGEFGLT
                     PHQAGFDEYLKDFHKMSDRNQWHWMYYSNDRGGWSPLNADGSETLIGPWLVRTYPKAT
                     AGKIKSFSFDTDTKIFEMTFVANAAIGEPTEIFMPNRHYPSGYDLEVNGTTNYTQNFN
                     SAKQVLSFSTTENDKEISINIAPK"
     gene            7628..8323
                     /locus_tag="PLL59_07720"
     CDS             7628..8323
                     /locus_tag="PLL59_07720"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMY23734.1"
                     /translation="MYAIKSYFLTLYTNWSAELKNAKFRNQFIITMLSYVFLFKYCRI
                     VMTIFESRKGVQINDKFLALLPPIDSSNTIFILTYSVLALFILTNITNPIKFAKAMQA
                     YTLLMIMRTITIYLVPLEPPVGMIILKDTISNFFMSGESGKYIVKDLFFSGHVSTAML
                     FFIITENKIIKRILLGLSICIATLILFQHVHYLIDVVAAPFFSLLAYTIVMLVHKESP
                     MLILFPNRYTKNN"
     gene            8382..10442
                     /gene="scpA"
                     /locus_tag="PLL59_07725"
     CDS             8382..10442
                     /gene="scpA"
                     /locus_tag="PLL59_07725"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HMY23735.1"
                     /translation="MSSDKKAYPTFEELSKAERTVDSVLFKHVDYVAGIAPFLRGPYS
                     SMYVNQPWTIRQYAGFSTAEASNAFYKRNLAQGQKGLSVAFDLATHRGYDSDHERVHG
                     DVGKAGVAIDSVEDMKILFNGIPLDKISVSMTMNGAVLPILAFYIVAAEEQGVSQHLL
                     SGTIQNDILKEFMVRNTYIYPPQASMKIISDIFEYTSKNMPKFNSISISGYHMQEAGA
                     TPEIELAYTLADGLEYLRTGIAAGLNIDDFAPRLSFFWAIGMQHFKEIAKMRAARMLW
                     AKIVSQFNPASEKSLALRTHCQTSGWSLTEQDAFNNVARTCIEAMAAALGGTQSLHTN
                     ALDEAIALPTDFSAKIARDTQIYIQKETEICRTVDPWAGAEYVEQLTEELAQKAWKLI
                     EEVESYGGMTKAIEAGIPKMRIEEAAAQKQALIDSGKDAIIGVNVFKTDDEQAIDILD
                     IDNAEVRENQIARLKSIKSNRNNIAVQKILQQLTEAAINNNNNLVALCVEAARCRATL
                     GEISSALESVYGRYKATIRSIAGVYSAEINMNEDFQLARQLSDEFAKEKGRRPRILVA
                     KIGQDGHDRGAKIIATAFADIGFDVDIGPLFQTPQEAAMQAAENDVHIVGVSSLAAGH
                     KTLIPQLIAELKKINRADILVACGGVIPKQDYDFLYQAGVVGIFGPGTVIAKAALHIL
                     EKLK"
     gene            10548..11222
                     /locus_tag="PLL59_07730"
     CDS             10548..11222
                     /locus_tag="PLL59_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23736.1"
                     /translation="MNKLSLRFSIFCFLLIGLLNVLQAQSNKYKDIIKIDGIKNTKYL
                     ATVSGIADVELFKPASFSFGMSFSGSIRLLYKPKLNQAKSVYSARVIEREMFIKPTLG
                     YIYRKRYNTALFFIPEIAYRHTFNNGIFVEVNFDAGYLFTKSNAPTYERQDDGTFKKV
                     FFGYHNVILGGKLNAGYDFSKKMKTPLAINFGTGLWYHYPSNEQWTRRIFVEAGLSYV
                     FRRNKE"
     gene            11227..12276
                     /locus_tag="PLL59_07735"
     CDS             11227..12276
                     /locus_tag="PLL59_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMY23737.1"
                     /translation="MKFNQFNIIYFFVAICLLTACSTSPKVPADQLDNYYFVKVGDIA
                     LPVRVCGNINADIAIVYVHGGPGGSAQGERANKYWEEIEKYYKVIYYDQRGSGITQGN
                     AHAEDMTIEQFSEDLDNIVDFTKQVAKANTVFIHGASWGGGLATYYLLDTTHQHKING
                     AIIEAPAYDIKNGAALSVQWMLHKADSMIALGNNLAYWNNCKDFYHAQTALSYKQFKQ
                     HQSYLTQVFGIVSNSINLQVRDISVPQFEIGLSYANAEFAPSKLTYEGQSIFTHLDLT
                     PKLNQIKTPILLIWGAKDGLLPKDNLAQKYALNIGSTDFTYDATKYLLSAHLPHFEEW
                     QQCNVDVKTFVEAHK"
     gene            12339..13841
                     /locus_tag="PLL59_07740"
     CDS             12339..13841
                     /locus_tag="PLL59_07740"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HMY23738.1"
                     /translation="MKKYFYLFLLATCSLLTVSCSVNPVSGKKQLSFMSESQELAIGK
                     ENDPAVIAQFGLYPNDTIQQFIKEKGNQMAAKSHRPTLPFEFRVLDSDVINAFAVPGG
                     YVYFTRGILAHFNNEAQFAGVLGHEIGHITARHSSQQYTKQMLTQIAFMGGMLLSEKF
                     RNISEQAMQGMQLMFLKFSRDDESESDVLGVKYSSAIGYDAKEMADFFKTLQRVTEKD
                     GARLPEFMSTHPDPGNRYNDVMKLATEYQTTNNLSGLKINRDAYLKMIDGIVYGPDPR
                     QGYLDKSNSIFYQPEMKFEYPIPRDWQFQNSPQAVQAAPKEGDAALILMLSSQNNFQD
                     ASAEMLEAYNLKLVANPISSKINNIDAYTFIAEQKQESNQYYGQQQPTQQTQNTDENL
                     LVHTTLYRYDGNIYILHGMCAKKDYAKYKNTFTYSMNGFKALTDPKRINVVPERIQIV
                     ALKNDATLKEVMIANKIPADRFEEVAILNGMQQNTIVKKGTLVKMLEKKL"
     gene            complement(13911..14810)
                     /locus_tag="PLL59_07745"
     CDS             complement(13911..14810)
                     /locus_tag="PLL59_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23739.1"
                     /translation="MKKKHVLIGIIAIATTIFSCKKENKEPTAASSLFYTVYSDSIIG
                     KIDLKNGNAISNFSKGIASGLNRGSLLGLALNTNTGEIYATNEQTNGPIYKINTNGIA
                     TILYDGANADRPAGIAYSSSTNKVYWSNRGDGKMYSISASGGTPTQMFVGFDDADGYS
                     VKVDDKNGKIYFANFDEIFIGNIDGSGTPTVLYSDKNDTLEAPSSIELDVDNNKIYWS
                     DEFADVVASANLDGSGNIKILFNNTTHGVDRSDGLAIDFVSKKIYWTETDGVGRIRVG
                     NLDGSGTPITLNSGFDTYNLLLK"
BASE COUNT         4961 a         2516 c         2754 g         4733 t
ORIGIN      
        1 gatttctgtc tttgtttttt agatagtttt tccaccactt taattcaaga ttttgtgcta
       61 atttccattt ggcagaagtg tttgtagtca aggatatacg tttatacaaa tttataattt
      121 ataaccgaca gttactacta ccaaaggatt ttttgtgctg agcgttgctg ctggcactgg
      181 attccaagct gttgtattga gctgatatgg cacatacatt tctttgaaat tggtttgcat
      241 gtaagcaagg tcggcaaaga aatgtttgcc acgataacca ataccagcag aaaaagtaag
      301 cacatcagtt gatagattgc ttggtgctgc acttttctta aatggtgaag tgcgatactg
      361 cacgccacct cgtatgcgaa caggatcgta tttaaattct atgccagctt ttatcgtatg
      421 aaatagagcg tatttgttgt caatgttggc attcacaaag cgctcatatt gattagcact
      481 ggcatcacta aatttgaatt tcgaattgcc agcatcactg agctcatatt ccatcgaaac
      541 aaaaccatat ttgtgaatat agccagcacc agttgttaat ttccaaggtt gaacatattt
      601 gtatttagag gcgccttcag gcgattcacc tgtatatatt acagattgat tcgcataatc
      661 aacttccatt tgcgttagaa atgcatcttt cataaatata atacttggcg tttgtatggc
      721 taaggataat ctcagatttg gtataggcga gccaattata ccaactttta gattaaagcc
      781 gacgccacgt gtttttaaat agctagcatt taaaaacgaa tttaggttgt atgccgaatc
      841 atttacatct ttttcttcta atcgtatgcg ctctgtgtag cttataaatg gaatgccaat
      901 tgacgcgcca agcattaatt tatctttata catgctagca atacccagcg ctagttcatc
      961 catgccacca gttttttgaa tgttgtatgt ttgctgcatg tggccattgt ttgtagctgt
     1021 aaaagtattg cccaaactgt ctatcgtaat taaacctaat cgataggcta tgctagcatc
     1081 gaaagggtat ttgtctgctg cgtcagtaga atctgttatg taatctctat cgtttaattt
     1141 ttcgtagtat gcattcagta aactattttt atcgttatag ccactaaaag taaaatcatt
     1201 attgtaattg gctaatctat taatagcaaa tccaaatttt aaaccattcc atttgccggc
     1261 attggatttg ttactgtttc gacctgcaat cacaatacct agattgctca attgaaactt
     1321 tactttatca tcttttgtgc tcgtatttaa aaaagtagat tttgatttag caatcgaaat
     1381 agctggcgta atagtaaact cagttgcgct aaattttgca atgcccgcag gattggtgcc
     1441 aagcgcacta atttctgcac cgatggtgcc catcgtattg cctactgcaa tatttcttgc
     1501 agttccttgt aacgaaggag tgctgtaacg tagcgcatca acatcagttt gggcgtttgc
     1561 aaaagctata agcattattt gcaagtaaaa taaaataact tgtttgcgca tttcagaaat
     1621 ttatacatta ttttgaacgt gtgccaattt taataccact atttccgtcg ttggtattta
     1681 ttttttcgcg ttcatagcta ttgttgttat aggaaggtgt attattccaa gaattattag
     1741 aatgattatt tggctgtgca tatttataca cattctcatt cggtgtattc gtcgatgaat
     1801 tcgattgctt agtattccat ttagtcgttt ttgttacttg ttgtgtgttt gtagtgcttg
     1861 aattgtcatt aaatccacct ttgccattat ttgtgtttga tggataatta ttgtattgat
     1921 tgtaattgcc atttcccgat tgaacattac ctgtgttgtt gtatccgcca gttctaggtg
     1981 tgtttacaac atttcttttg ttataataat tctcattaat gccataatgg tgataatatg
     2041 aattattata aatagcattc gaacaataag gtgaaaatcc gtaccagttg ttgttccaag
     2101 aattccaacc aaagttattc caagcattcc aaggattatt atagcctgta ttgtaccaac
     2161 tgctatatcc aaatccaggt tgtataatta taatttgtgt cgaattaaat ggattataaa
     2221 aattagcata actatgatac caacttggcg taaaggaata attgtcccaa ttattccaac
     2281 cgaagttgtt attgtatgaa ttccaaggat tgtagtagtt attattccaa ttgttccagc
     2341 cataattaaa ttggaaagat ggattgtgga atcttctaat tctgtctgta tagctgtaat
     2401 tccagtcgct gtcatagcca ttattataat cagtgtaatt gtcactataa ttatcagcaa
     2461 tgaaattttt atcagcaatt cgagtcgttg tttgctcgct ttccgtataa ggttggctaa
     2521 tttcagttga aatctttttc tcagatggtt ctgtaggttc tgtatatgtt ttttttactt
     2581 tgctttgttt tggcctcgga ttgtcatata catcatcttg tgctaatgat atatttgctg
     2641 gcaaaataaa tatggcaagc gtcataaaaa ctactatatt tttcatcgca tacattttta
     2701 atcttttaga aatctaaaat ctcgtacttt tataaaccaa aattacacat aaacggtttc
     2761 aaaaaacata ccaaattatg ttaattaaca tctaattcaa caatatgagc aaagaaatta
     2821 ccagtagagc agaaaattat tcagaatggt ataatgattt agtattaaaa ggtggcctgg
     2881 cagattattc cgctgtgcgt ggatgtatgg taatcaagcc ttatggattt acactttggg
     2941 aaaatatgcg agatgtattg gacaagatgt ttaaagaaac aggtcatgtg aatgcctatt
     3001 ttccattgtt tgttccaaaa agtttatttg aagcggaaga aaaaaatgca gaaggatttg
     3061 caaaagaatg tgctgtagtt acgcactatc gattaaaaac aaatcctgat gaaccaggca
     3121 aactaatagt cgatccagaa gctaaattag aagaggaatt agtcgttcga cctacatctg
     3181 aagccattat ctggaacacc tacaaaaagt ggattcaatc ttatcgcgat ttaccaattt
     3241 tagttaacca atgggcaaat gtcgtgcgct gggaaatgcg cactagattg tttttgcgta
     3301 cagcagagtt cttgtggcaa gaaggtcata ctgctcatgc tacaaaagca gaagcggtgg
     3361 ccgaaaccat acaaatgata aatgtatatg cagattttgc agagaattat atggcattgc
     3421 cagtcatcaa aggcgtaaaa tcagaaagtg aaagatttgc aggcgccgaa gatacttaca
     3481 ccattgaggc catgatgcaa gatggaaaag cattgcagtc gggcacatct catttcttag
     3541 gacaaaattt tgcgaaagca tttgatgtta cctatgtcaa caaagacaac cagcctgaat
     3601 atgtttgggc tacttcttgg ggcgtttcta cacgattaat cggtgcatta ataatggcac
     3661 acgctgatga tgatggctta gtaatgcctc cgcgaattgc acctcatcaa gtggtgataa
     3721 ttccattttt gtctaaaaat gaagaggaaa cacagcgcat gattactaca gcaaatgaac
     3781 taaaaagtga cttgcaaaag ttaggaataa gagcgattgt tgacagtgac gaaacaaaac
     3841 gtcctggttt taaatttgca gaatatgaac taaaaggaat tcctatccga gtcgttattg
     3901 gtccacgcga tttagcaaat ggaatggtgg aaattgctcg cagggataca aaagaaaaat
     3961 catcttattc ggttgaaaat ttacctaaat caattgaaca attattggat aacattcagc
     4021 aaaatttatt tgataaagca aaagcattta gagatagcaa tattactaaa gttgatgatt
     4081 ttgcaacttt ccaaaaatta ctcgatgagc aaccaggttt tatattagca cattgggacg
     4141 gcaccgctga gacagaagag aaaattaaag aattgaccaa agcaacaata cgctgtattc
     4201 cattagacaa tccacaagaa gatggaaaat gtatattaac aggtaatcca agtaaacaaa
     4261 gagttttgtt tgcaagagcc tattaatttt tttgattata ctattttcag caataaaaat
     4321 gctgcattta tgataaagaa tttaatactc gaacttcctc cagaagtagc atatgatgaa
     4381 ataatttttc atcagtatat taaaaattat ttacaaataa atgatgcaca agaatatcat
     4441 attcgcatct ctaaacgttc tattgatgca cgttcgcgaa acatcaaagt caatatcaat
     4501 gctaccgttt ttatagatga gcaacccgat tcaattgtgt caccaatcca atataagaat
     4561 gttgcaaatg caaaatcagt aattatagta ggagcagggc ctgccggttt gtttgccgca
     4621 ttgcgattga tagaattagg tgtaaaacca atagtgattg aaagaggaaa agatgtacaa
     4681 gatcgaagac gtgatttagc caatatcaat aaaaaaaata tagtcaacga aaattctaac
     4741 tattgttttg gcgaaggtgg agcaggcaca tatagcgatg ggaaattata tacacgctca
     4801 aaaaaacgtg gagatataaa taggatttta gaaattatgg tgacacatgg tgcgcaagaa
     4861 aatatcttgt tcgaagcgca tccacatatt ggcaccaata aattaccaaa acttattcaa
     4921 gcaattagaa ataccatttt ggaatatggt ggtgaaatac attttaacac caaggtagta
     4981 gattttattg taaacaagaa tgaaatgaaa ggcgttgtcg ccgttcgtac agatacatta
     5041 caaacattat ctacgacgcc aaatatagaa tatataggca taggcgtaat attggcaaca
     5101 ggccattctg ctcgagatat ttttgaattg ttgcaccaaa aaaatattct aatagaatcc
     5161 aaaccttttg ccttaggtgt acgcatcgaa catcaacaat cattaatcaa ccaaatacaa
     5221 tatcatggta atgattgcac gatattgcct gccgcaacat atagcttggt tgagcaagtg
     5281 aaagttggaa caacgcaaaa agcggtgttt tctttttgta tgtgccctgg tggttttatt
     5341 gtgccttcag ccactcatca agaagaaatt gtcgtgaacg gcatgtcgcc atctaagcgc
     5401 gattctcctt attccaattc tggaatggtg gtcgccatta acgaattaga ttggaaagat
     5461 tttaatcact tggggccttt agcagcattg gcatatcaaa aaaatataga acagaaagcg
     5521 tggcagattg caggtcgcac gcaagcagca ccagcacatc gaatgttgga ttttgtgaaa
     5581 aataaaccat ctgatgattt gttggatact tcgtatcagc ctggattagt cagtgtggat
     5641 atgcatgata ttctaccatc aacaattgca tattctttgc aagaaggctt tacaagtttt
     5701 ggaaaaaaaa tgaaaggata tcttagcaat gatgcacaaa ttattggcgt cgaaagtcgg
     5761 acatcatcac ctattaaaat tccacgcgac aaagaatcat tagaacatat cacgattaaa
     5821 agattgtttc catgtgctga aggtgcaggc tatgccggtg gcattatgag tgcagcgatt
     5881 gatggtgaac gatgtgctga aaaattagtt gctttgtatg caagcttata gcgtgttgct
     5941 ttgctaggat tttttgtttt cttctttttc tataataaat tcttcgactt tgcttcgata
     6001 aatatatttt ctcttgctaa atgtcaatta attgtagatg aaatgagatt tcgaggaaat
     6061 aatcagtcat aaaataccaa tttatggtga tataacttaa tttagtaaaa tgaaacgatt
     6121 acttttttta gttgccatca ctgctttctt attttcctgt aaaaaagaaa gtgcagaacg
     6181 tcaacgcaat cagatagcca acgatcctac gattattcaa gaccagtatg gtcgccagct
     6241 tattcttcat ggtttaaata cagcttcaaa tgcaaaatca gactctttgc gcaatccatg
     6301 gatttcagaa agtgatgtag aaagagaagt aaatattttt ggcttcaatg tagtgagata
     6361 cttaatattt tgggacgcta tagagccaca aaaagatttt tttgatgatg cttatttaga
     6421 cagagtggaa gaacgagtaa attggtattc atcacgtggt atgtacgtga tgttagatat
     6481 gcaccaagat atgtattcca ttatatttgg tggcgatggt gctccggcat gggcttgccg
     6541 taccaatggt gcagctccaa tagaattacc aggtggaacg ccttggtggt tgaaaaatat
     6601 cgacccacaa gtagtcaatt cttggattaa tttttgggct tatactaatc ataaagactt
     6661 acaagaccat tacattttag catggaaaaa agtaatagaa cgattcaaaa ataatccata
     6721 cgtaattggt tatgatttga tgaatgagcc ttggggcggc gacttaataa aagtatttat
     6781 aacaggtgag tttgagcgtt atcaattaac agcattttac aatcgcttga ttccagcatt
     6841 acgcgcagca gacccagata agtatatgtt cttcgagcca acacctgcgc cagttacttt
     6901 tggtatgcct tctaatttgc gtaagatcag tgataccaga agctcatcca gaactgtata
     6961 tgctccacat tgttatcctt tcgatacaca cgaaggcaca ggctatacag cggcttcaaa
     7021 aagccagttg atagattttg aaagagagcg aaagaaagaa tcacaactac atggtaatgt
     7081 accagtgcta atcggcgagt ttggcttgac gccacatcaa gctgggtttg atgaatatct
     7141 taaagatttt cataaaatgt cagacagaaa ccaatggcat tggatgtatt atagtaatga
     7201 tagaggtggc tggtcgcctt tgaacgctga tggttcagaa actttaatcg gaccatggtt
     7261 agtacgcact tatccaaaag caacagcagg gaaaattaag tccttcagct tcgatacaga
     7321 tactaaaatt tttgaaatga cttttgttgc caatgctgct attggagagc caacagaaat
     7381 atttatgcca aataggcatt atccatctgg atacgatttg gaagtgaatg gcactacgaa
     7441 ttatacacaa aattttaatt cggcaaaaca agttttatct ttctccacaa ctgagaacga
     7501 caaagaaatt agcattaata ttgctcctaa ataagcgcac tttttttgtt aaaatttagt
     7561 gaaacctatt tgtcatattt gaagaaatta tgtatattta ctacttttga aaaaaaagta
     7621 tagataaatg tacgccataa aatcatattt ccttacatta tataccaatt ggagtgctga
     7681 acttaaaaat gcaaagttca gaaatcaatt tattatcacg atgctgtcgt atgttttttt
     7741 atttaaatat tgcagaattg taatgacaat atttgaaagt agaaaaggcg ttcaaataaa
     7801 tgataaattc ctagctttat tgccaccaat agactcctca aatactattt tcattcttac
     7861 gtattccgta ttagcattat ttatattgac aaatattact aatccaataa agttcgccaa
     7921 agcaatgcaa gcttatactt tattgatgat tatgcgtaca attactattt atttagtgcc
     7981 tttagaacct ccagtaggta tgattattct aaaagatacc atttcaaact tttttatgag
     8041 tggcgagagc ggaaagtata tcgtcaaaga tttatttttc tctggtcacg tgtcaacggc
     8101 aatgctattt tttattatca ctgaaaataa aattatcaaa cgtattttat taggcttaag
     8161 tatttgtatt gcaactttaa tattgtttca acatgtacac tatttgatag atgtcgttgc
     8221 agcacccttc ttttctttat tggcatatac tattgtaatg ttggtacata aagaaagtcc
     8281 aatgctgatt ttatttccaa atcgatatac aaagaataat taattcattg cttttttgaa
     8341 gaaatattca attaaaacga tacactccgt atctttacta catgtcatca gataaaaaag
     8401 cttatccaac atttgaagaa ttatcaaaag cagaaagaac tgtcgattct gttttgttta
     8461 aacatgtaga ttatgttgca ggcatcgcgc catttttgcg aggaccatat tcttctatgt
     8521 atgtcaacca accatggaca atacgccaat atgcaggatt ttcaacagca gaagcatcga
     8581 atgcatttta taaacgaaat ttagcgcaag gtcaaaaagg cttgtccgtc gcattcgatt
     8641 tagcaacaca tagaggttat gacagcgacc atgaacgagt gcatggcgat gtaggcaaag
     8701 caggcgtagc catcgattct gtagaagaca tgaaaatatt gtttaatggt attccattag
     8761 ataaaatttc agtgtctatg acaatgaatg gtgctgtgtt gccaatttta gcattttata
     8821 ttgtggccgc cgaagaacaa ggtgtatcac aacatttatt gagtggtacg atacaaaatg
     8881 acattttaaa agagtttatg gtgcgtaata cttacattta tccgccacaa gcctctatga
     8941 aaattatctc tgatattttt gaatatacca gcaagaatat gccaaagttt aattcgattt
     9001 ctatcagtgg ttaccacatg caagaagcag gtgcaacacc agaaattgaa ttggcataca
     9061 cattagcaga tggcttagaa tatttacgta cgggcattgc agcaggtttg aatatagatg
     9121 atttcgcacc acgactttct ttcttttggg caattggtat gcaacatttt aaagaaattg
     9181 ccaagatgag agcagcacgt atgttgtggg caaaaattgt atcacaattt aatccagcat
     9241 ccgaaaaaag tctggcgctt cgtacacatt gtcaaacttc tggttggagt ttaacagagc
     9301 aagatgcttt caataatgta gcacgtactt gtattgaagc aatggctgct gcacttggtg
     9361 gcacacaatc acttcatacg aatgcattag atgaggcgat tgctttgcca acggatttct
     9421 ctgcaaaaat tgctagagat acacaaattt atatacaaaa agaaacagaa atttgtagaa
     9481 cagttgatcc ttgggcaggt gctgaatatg ttgaacaatt aacagaagaa ctagcacaaa
     9541 aagcttggaa attaatcgaa gaagtggaaa gttatggtgg catgacaaaa gcaatcgaag
     9601 ccggaattcc taaaatgcgc atcgaagaag cagccgctca aaaacaagca ctcatcgata
     9661 gtggaaaaga tgccattatt ggtgttaatg tatttaaaac tgatgatgaa caagcaattg
     9721 atatattaga tattgacaat gcagaagttc gcgaaaatca aattgcgcgc ttgaaaagta
     9781 ttaagtctaa tcgaaacaat atagcagtac aaaaaatatt acaacagctt acggaagcag
     9841 caataaataa taataataat ttagttgcac tatgcgttga ggcagcacgt tgcagagcca
     9901 cactcggcga aatctcgtcg gcattagaaa gtgtgtatgg acgatacaaa gcaaccataa
     9961 gaagtattgc aggtgtctat tctgctgaaa taaatatgaa tgaagatttt caattggctc
    10021 gtcagctaag cgacgaattt gcaaaggaaa aaggccgccg acctcgtata ttagtcgcta
    10081 aaattggtca agatggacac gatagaggtg ccaaaattat agcaactgca tttgcagata
    10141 tcggctttga tgtggacata ggaccgcttt ttcaaacacc acaagaagcc gcaatgcaag
    10201 ccgcggaaaa tgatgtgcat attgttggcg tttcatcatt agcagcagga cacaaaacat
    10261 taattccaca attgatagca gaattaaaaa aaataaatcg tgctgatata ttagtagctt
    10321 gcggtggcgt tattccaaaa caagattatg actttcttta tcaagcaggt gtagtaggta
    10381 ttttcggacc aggcacggtc atcgcaaaag cagcattaca catcttagaa aagttgaagt
    10441 aaatctattt ttatcacaat atgtaatgtt ttccaactta ttatagcgca aatacaaatt
    10501 aactgctatt ttttatttat tttgcacctt tatttatata cattcagatg aataaactgt
    10561 ctttgcgctt tagtatattt tgttttcttt taataggatt gttgaatgtg ttgcaggcgc
    10621 agtctaataa atataaagac attatcaaaa ttgatggcat caagaacaca aaatacttgg
    10681 ctactgtctc tggtattgct gatgtggaat tatttaaacc agcttctttc agcttcggaa
    10741 tgagctttag tggcagcatt cgtttattgt ataaaccaaa attaaatcaa gcaaaaagtg
    10801 tgtatagtgc tcgagtaata gagcgcgaaa tgtttatcaa acctaccttg ggttatattt
    10861 atagaaaaag atacaataca gcattgtttt ttattccaga aatcgcatat cgtcatactt
    10921 tcaataatgg gatttttgtg gaggttaatt ttgatgcagg ttacctgttt accaagtcaa
    10981 atgcgcctac ctatgaacgg caagatgatg gcacatttaa aaaagttttt ttcggctatc
    11041 acaatgtaat tctaggtggc aagctgaatg caggatatga tttttctaaa aaaatgaaaa
    11101 caccattagc cattaatttt ggcacaggct tgtggtatca ttatccttca aatgaacaat
    11161 ggacaagacg tatttttgta gaagctggtt tgtcgtacgt atttagaaga aacaaagaat
    11221 aagaacatga aatttaatca atttaatatc atttattttt ttgttgcaat ttgcttattg
    11281 acggcatgct ctacatctcc aaaagttcca gcagatcaat tggataatta ttacttcgta
    11341 aaagtaggtg atattgcatt gcctgttcga gtttgtggaa atatcaatgc ggatattgct
    11401 attgtatatg tacacggcgg acctggcggt tctgctcaag gtgaacgtgc taataaatat
    11461 tgggaagaaa ttgaaaaata ttataaagtc atttattacg atcaacgagg ttctggtatt
    11521 acgcaaggaa atgcacacgc agaagatatg acgatagagc agtttagcga agatttagac
    11581 aatattgtag atttcactaa acaagtggca aaagctaata ccgtttttat tcacggcgca
    11641 agttggggtg gaggcttagc aacctattat ttacttgaca ctacacatca acacaaaatt
    11701 aatggcgcca ttatcgaagc acctgcatac gatataaaaa atggtgctgc tttgtctgta
    11761 caatggatgt tgcataaagc cgattccatg attgcactcg gaaataactt ggcatattgg
    11821 aataattgta aagattttta tcatgcacaa actgcattat cgtacaagca atttaaacaa
    11881 caccaatctt acttgactca agtattcggt atcgtttcca atagtattaa tttacaagtg
    11941 agagatatta gcgttccgca attcgaaata ggtttgtcgt atgcaaatgc agaatttgca
    12001 ccatcaaaat taacgtatga aggacaatct atttttacac atctagattt gacaccaaaa
    12061 ttaaatcaga ttaaaacacc aatactatta atttggggcg caaaagatgg actattgcca
    12121 aaagacaatc tggctcagaa atatgcacta aatattggca gcacagattt tacttacgat
    12181 gcaacaaaat atcttttatc cgcacacttg cctcattttg aagaatggca gcaatgcaac
    12241 gttgacgtaa aaactttcgt cgaagctcat aaataataca ctatactgta taaatggtat
    12301 aattattgca tttcttatct cgattaatat tcatatttat gaaaaaatat ttctatctat
    12361 tcttgcttgc tacatgttca ttgttgaccg tgtcgtgttc cgtaaatcca gtttctggca
    12421 aaaagcaatt aagctttatg tctgagtcgc aagaacttgc cattggcaaa gaaaacgacc
    12481 ccgcagtaat agcacaattt ggattgtatc cgaatgatac cattcagcaa tttatcaaag
    12541 aaaaaggcaa tcaaatggca gcaaaatcgc atagaccaac tttaccattt gaatttagag
    12601 ttttagattc tgatgtcatt aatgcatttg ccgtacctgg cggatatgtt tattttacac
    12661 gcggaatctt agcacatttt aataatgaag cacaatttgc aggcgtgtta ggacacgaaa
    12721 ttggacacat caccgcaaga cattcttcgc aacaatacac caagcaaatg ctgacgcaaa
    12781 ttgcatttat gggtggcatg ttgttgtcag aaaaatttag aaatatatca gagcaagcca
    12841 tgcaaggtat gcaattgatg ttcttgaagt tctcgagaga tgacgaatca gaatctgatg
    12901 ttttaggcgt aaaatattca agcgctatcg gctatgatgc aaaagaaatg gccgactttt
    12961 ttaagacatt acaacgcgtt acggaaaaag atggtgcgcg tttgccagag tttatgtcca
    13021 cacatccaga tccaggaaat agatataatg atgtgatgaa attagcaacg gaatatcaaa
    13081 ccaccaataa tctttcaggc ttaaaaataa atagagatgc ttatttaaag atgattgatg
    13141 gtattgttta tgggccagat cctcgtcaag gatacttaga taaatcaaac tccattttct
    13201 accagcctga aatgaaattt gaatatccaa ttccgcgcga ttggcaattt cagaactcgc
    13261 cgcaagctgt gcaggctgcg ccaaaagaag gtgatgctgc tttaatatta atgctttctt
    13321 ctcaaaataa tttccaagat gcaagtgctg aaatgttgga agcttacaat ttaaaattgg
    13381 tcgctaatcc gataagcagt aaaattaata acatagatgc ttatactttt attgctgagc
    13441 aaaaacaaga aagtaaccag tattacggac aacaacagcc aactcaacaa acacaaaata
    13501 cagatgagaa tttattagtg catactacac tttatcgcta tgatggcaat atttatatac
    13561 tacatggcat gtgtgctaaa aaagattatg caaaatataa aaatactttc acctattcga
    13621 tgaatggctt taaagcatta acagacccta agcgcatcaa tgttgttcct gaaagaatac
    13681 agatagttgc ccttaagaat gatgccactt taaaagaagt gatgatagca aataaaatac
    13741 ctgctgacag atttgaagaa gtagctattt tgaacggtat gcaacaaaat acaattgtga
    13801 agaaaggtac actggtaaaa atgctagaga agaaattgta atgtgaaata acgtagagat
    13861 aaaaaaaaga gccaaattct ggctcttttt tttatggaat aatactgatt ttatttcaat
    13921 aacaaattat atgtatcaaa gccactattt aaagtaattg gcgtgccact gccgtccaag
    13981 tttccaactc ttattcttcc tacaccatca gtctcagtcc aatatatttt tttagaaaca
    14041 aaatcaatag ccaaaccgtc tgacctatct acaccatgag tagtgttatt gaataatatt
    14101 tttatatttc ctgagccgtc taaattagct gatgcaacaa catctgcaaa ttcgtcagac
    14161 cagtatattt tattattgtc cacatcgagt tcaatactag atggagcttc taaagtatca
    14221 tttttgtcgc tatataaaac agtaggagtg ccgcttccat ctatgttgcc tatgaatatt
    14281 tcatcaaaat ttgcaaaata tatttttcca tttttatcat ctacttttac agaatatcca
    14341 tcggcatcat caaaacctac aaacatttgt gttggtgtgc caccagatgc actaatacta
    14401 tacattttac catcacctct gttagaccaa taaactttat tggtactgga agaataggca
    14461 attcctgctg gtctgtctgc atttgcgcca tcatacagaa tcgtagctat accattagta
    14521 ttaattttat agataggtcc gtttgtttgt tcatttgtgg catatatttc acctgtgttc
    14581 gtgtttaatg ccaaaccgag taatgaacct ctatttaacc cagatgctat acctttagaa
    14641 aaatttgaaa tggcattccc attttttagg tctattttcc cgattataga gtcactatat
    14701 actgtgtaaa ataagcttga tgcagctgtt ggttctttgt tttctttttt acaagaaaag
    14761 atagttgttg cgatggcaat tatgccaatt aatacatgtt tttttttcat aacttttttg
    14821 atttataaat gaagataata catagatatg aatttgtcaa taaaaataat cctatttttg
    14881 cctaattatg aagtacaaat ccttacaaga atgtattgat gatttagata aatcaggtca
    14941 attgattcgc ataacagaag aagt
//
LOCUS       DAOQQV010000064        14445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1365_length_14445_cov_7.533426, whole genome shotgun sequence.
ACCESSION   DAOQQV010000064 DAOQQV010000000
VERSION     DAOQQV010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14445
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1365_length_14445_cov_7.533426"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1804)
                     /locus_tag="PLL59_07750"
     CDS             complement(176..1804)
                     /locus_tag="PLL59_07750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HMY23740.1"
                     /translation="MAKSKFLIILSFMAVFSVQAKINTSKYIIYFKDKTFDINVKALF
                     NTKAIQQRNRFNIPFDERDYPVNANYIEQLKQNNAIILHTTNWLNAALIRIEDKKLAT
                     IAQFPFIASIVKIETAKSHKNTMSVATQDACVGNEDELAFEDQYTSSFPQVNLLHGEY
                     LHEQGFNGENMTIAVCDNGFYNVYNNPAYAAVMADNRLLGTYDFVNNDSTVHDDTNGK
                     HGTNCFSFIAGMKDSQYIGTATKSSYYLFQTENNNSERLQEEFNLASALERCNQLGVD
                     VVSISLGYTTFDVASENHDTTDLRNNSTPAAKAVNMASSKGILVCVAAGNEGLKPWKY
                     ISTPSDADSAFCIASVDINGVVAGSSAYGLPNDARVKPNVAAMGVSAKYLNDAGNVSS
                     GGGTSYATPQLAGLAACLWQAFPTKTNWEIKTAIEQSASQYLTPDKRIGYGIPDFKKA
                     YDLLANPTFVSTKSIIDDIHVFPNPFQNILAVQCNGNANLQSIQLMNSVGQMVYSAYT
                     PTNNMLDFSYLSTGMYLLQITTDKGVFIKKIIKE"
     gene            complement(1791..2531)
                     /locus_tag="PLL59_07755"
     CDS             complement(1791..2531)
                     /locus_tag="PLL59_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23741.1"
                     /translation="MKYKFTIFLFFCFTLIAIISCKKETDVSKYDIGYDYFPNEYGNF
                     VIYKVDSVLFNDFDNSRRYNTVYLKEKIGEQFVDNLGRTANKILRYYADSLTTNWEIF
                     NVDYLVKTNLVAERVEDNLRYIKMVFPNDVGLKWLGNKFITVPPPFVLDTVNYFLDDW
                     KYTIKARDAYYNNGTRIFDSTLLITHIQDSTAITKTYSAERYARSIGMVYKEFWLVEG
                     QINIGAPWEDRAEKGFILRQYAIDYGKE"
     gene            complement(2562..3302)
                     /locus_tag="PLL59_07760"
     CDS             complement(2562..3302)
                     /locus_tag="PLL59_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23742.1"
                     /translation="MKKIPLSFLVLVLLFVSCKKETETVTFDFGYNYFPDDSGTYVIY
                     KVDSVLYNNFDPLNLKRTSSHYLKEIITEQFIDNLGRRAKKVERFVTDSLNHSWVPHN
                     VWYIVKNTTNAERVEDNIRYIKVTFPVSVGNIWRGNKYNELNHFPYTDLKFTATNFDW
                     NYEVKQVNQNYTFGSIITDSTMTVLQVNDSSNVQKVYSVEKYAKNIGMVYKELWRLDA
                     QLVNNQTYENNAVFGFILKQYAVEYGRE"
     gene            3581..4063
                     /locus_tag="PLL59_07765"
     CDS             3581..4063
                     /locus_tag="PLL59_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23743.1"
                     /translation="MNNQQTVGTAFKTISIFHIAMCVGLIAIVAVFRFIVKQDNTAST
                     PNQTMEIIGIAVGFIGIISARLLFFKQVKTAHTVSALKDKIAIFRNAFIIQMAILEAA
                     ALLNAVLYFITKNDMHFFIAVGIILLMIVRRPTRAMAGMTLFTSMEDKQVIYNDSTPL
                     "
     gene            complement(4064..4546)
                     /gene="rraA"
                     /locus_tag="PLL59_07770"
     CDS             complement(4064..4546)
                     /gene="rraA"
                     /locus_tag="PLL59_07770"
                     /inference="COORDINATES: protein motif:HMM:NF006875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HMY23744.1"
                     /translation="MSFSTADLYDANEGKVHVAMSIFQNYGLKKQFHGQIYTIKCFED
                     NTPVGETLRNENGKGKVLVIDGGASLRCALVGDLIAAAAIKNEWEGIIVNGCIRDSAV
                     INTMQIGIKALNTNPTKSVKKYPGLKNIIVDFAHVIFHPNQFIYCDEDGILISKNALL
                     "
     gene            4638..5567
                     /locus_tag="PLL59_07775"
     CDS             4638..5567
                     /locus_tag="PLL59_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HMY23745.1"
                     /translation="MAKQYTSSLMMVRPASFQFNIETAQSNAFQQHLEGLSKEEIKCK
                     AIEEFDAYVEKLREHKIDVTVIQDTPNPAKPDAIFPNNWISMHADGTIYLFPMCTANR
                     RLEIRPEIIKEIEKKFVVNKVDNMSALANDKLFLEGTGSIIFDHIHRKAYACISPRTD
                     KKLLEEFCLKIGYQAITFTSVDLQNKLVYHTNVMLTIGDTFAIICLESIRDERERELV
                     KQHLANSGHDIIDITFEQMQSFAGNMLQVQNTDGKTYVVMSETALDSLTATQIKQIEM
                     HTAILSVEIPTIETIGGGSARCMLAEIFLKEKN"
     gene            complement(5564..6040)
                     /locus_tag="PLL59_07780"
     CDS             complement(5564..6040)
                     /locus_tag="PLL59_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMY23746.1"
                     /translation="MPVIQLTTEIAAPIDICFDLSRSIDLHKYSMKHTNEEAVAGRTS
                     GLIEDGETVTWQAKHLGVTQYLSSKISNFKRPFIFRDSQTKGIFDFYHHDHIFEEKNG
                     KVVLIDIFEYKSPFGIFGIIADMLYVKKYMIKLLQQRNQVIKEFAESDKWKSILSR"
     gene            complement(6101..7276)
                     /locus_tag="PLL59_07785"
     CDS             complement(6101..7276)
                     /locus_tag="PLL59_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HMY23747.1"
                     /translation="MSNTNRYDQRGVSATKDEVHAAIKNADKGLFPNAFCKIFPDYLT
                     LDENYCSIIHADGAGTKSVLAYLYWKETGDLSVWKGIAQDAIVMNTDDLICVGATSNF
                     LMSSIINRNKHLIPGEVIKALIDGTNEVVEMMHENGVNIHAMGGETADMGDVIRTVSV
                     DAVMTARFKRADVIDNANIKPGNVIVGLASYGQASYENEYNGGMGSNGLTSARHDVFS
                     KIYAQKYPEAFDPNTNMDFIYAGTKLLTDKVENSPLDAGKLVLSPTRTYAPIVKRVID
                     ELGVKNINGIIHCSGGAQTKILHYVDCLHIVKNNLLAVPPLFALIQEESKTDWKEMYQ
                     VFNCGHRLEIYCAPEHAEIIISIANSFAVEAQVIGYVEAADKKQVTISSDKGEFIYH"
     gene            complement(7287..7694)
                     /locus_tag="PLL59_07790"
     CDS             complement(7287..7694)
                     /locus_tag="PLL59_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMY23748.1"
                     /translation="MQRINNLSASSQRKWGKMSVSQMLKHMTTAFAVPIEKIQVPREP
                     LYYVVANPIARWMMIKAMTKWPKNMMTAQAFLVKEDIDFEPAKKSFLETYQAFLQASS
                     FNGSHPIFGTMSKELWGDAMYIHLNHHLEQFGV"
     gene            complement(7848..9809)
                     /locus_tag="PLL59_07795"
     CDS             complement(7848..9809)
                     /locus_tag="PLL59_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY23749.1"
                     /translation="MKRICNILCIGIVLVLASCKSSSKVQDGETAFKIKKYTLAAEML
                     QKDYSKTKDIAQQSKIALKIAQSYDKQLNYIAAETWYKKIADARVLDNSLMLYVNALK
                     RNEKYTEALKTLEDYLKANKTEKFRLQKEYDFLQAAVKQTKAKQHTKLSNLEINTESI
                     DFSPFLKDNILYFSSTRGGGKNNTDDWTGEGYADIYSSQKLNDIAFNSPVNIGTPFNS
                     PYHDANIIFTKNGKTAFFTRCGSANKNVDEYCHIYRVDKNIDDSWSTPQRVKLFADTI
                     NEGQAYLSTDENELYFSSDNKDGYGGRDIYFAKKNIAGEYENPINAGSKINGQGDELF
                     PVIANDNTLYFSSNSKIGYGGLDIFAATKEGKIYTNVQNLGFGVNSGADDLSLQLTKV
                     IEDSVLLNGYLVSNRSGGKGKDDIYYFVKRVPPKEPLPPTVYVLNVHVQENNYENPNN
                     PSSKLLGQIPVENAIISLPKVDKMKTNSVGKIQSIIPANVEFTYNITKDGYLSQVDAV
                     STFANNEAKDGDTVIIERNIILSKIYKNVEIVLENIYYDYDKWDIRADATESLTLLVD
                     VLEKNPNIKIELASHTDCRGKDAYNETLSQKRAESVVKYLIGKGIDANRLTAKGYGET
                     MPIEKCDCETCTEEQHQKNRRTTFKIVSE"
     gene            complement(9990..12182)
                     /locus_tag="PLL59_07800"
     CDS             complement(9990..12182)
                     /locus_tag="PLL59_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HMY23750.1"
                     /translation="MSKTRFNALSAFSSRPKVNVELPSEKISDFFASAVFGEKEMREF
                     LPSDAYKKLAASIKDGKKVDREIADQVASAMKAWAMKQGATHFTHWFQPLTGTTAEKH
                     DSFFMPKGGSGIEEFSSDALVQQEPDASSFPSGGIRVTFEARGYTAWDVTSPAFIMTV
                     GSGKTLCVPTIFISYTGETLDYKAPLLKSIQALDQAATEVAHYFDRDVKHVYTTLGWE
                     QEYFLVDSAMYYARPDLVMTGRTLLGRRSPKGQQLEDHYFGAIPERVYAYMRDFEIEC
                     LKLGIPVRTRHNEVAPSQYECAPMFEEANLAVDHNSLLMDVMDRVSSKHKLKVLFHEK
                     PFAGINGSGKHNNWSMGTDTGVNLLSPGKTPRTNLQFLTFFINTIAAVNRHEELLRAS
                     VATHGNEHRLGANEAPPAIISVFTGSYLKEVLEMIESRVDEDKFDEQDNITLRLDIHN
                     RIPDIMLDNTDRNRTSPFAFTGNKFEFRAVGSSANCALPMTVLNAIVAEQLKLFKVEV
                     DEYIKGGDYKDVAILKVLRKTIKDAKRIMFEGDNYSDDWHKEAKKRGLSNNKTAPVAL
                     GAFVSDSSVKLFADLGIYSHREVEARYEIALENYIKAIQIESRALGEIVINQVIPACL
                     EYQNKLITNIRGLKEIGIDAKSYSAQSSLLKQISEGIEKVIKLEKDMLDLRVKANDLS
                     NALKQAQIYSDKVRPIMDEIRDVTDNLETIIDDELWPLPKYREMLFTK"
     gene            complement(12258..13529)
                     /locus_tag="PLL59_07805"
     CDS             complement(12258..13529)
                     /locus_tag="PLL59_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23751.1"
                     /translation="MLQELLAPTFYFLLFLFLISNKRLFPVPSISRNVLYIGFSLKVF
                     SALLFGFFFRNGILTGNDTFLYFYDGEIIYKTLKTDPFIYFKLAIGSNDFKPVPAYLL
                     PYTDAMHFWFDSSNYFIVRLNALLRPFSFGVYNVHAIIFAFLSFIGTYNLYLFFQDKV
                     RHLKMVQFILFGIPSIVFWTSGIHKESIVIFALGIILYNMDIVLTAKHTNRNILFSIA
                     GLFILGYTRIYLLAFLLPLMGSIALYNKLEIKTSYLKVIALSAMLLVFVLLLLDLIIP
                     NFSLTHEFLSRRTYFLNSPGNMSFQVEHLPHNFKGVLILLWEAFTNPFIRPLPNDCNY
                     FLAYLASFESIILLLTIVALLFSSNLKLVIKNPYALFCILFGLSTLFLIGLIVNNSGA
                     IVRYRSIAIPFILIGLCLVRQENKQTVLNNS"
     gene            13628..>14445
                     /locus_tag="PLL59_07810"
     CDS             13628..>14445
                     /locus_tag="PLL59_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HMY23752.1"
                     /translation="MSTPIQQPQVNEEIAKSLGLLPEEYQKIQDILGRIPTFTELSVY
                     SVMWSEHCSYKNSIKYLKKLPRKGKALLVEAGEENAGLVDIGDGWACAFKIESHNHPS
                     AVEPFQGAATGVGGISRDIFTMGARPIASLNSLRFGNLENKRTQHLLKGVVKGISHYG
                     NCFGVPTVAGEVYFDDCYQVNPLVNAMSVGIVKVGETISATSAGVGNPIYIVGASTGK
                     DGIHGATFASEDLGEDAEDKIPSVQVGDPFTEKLLLEATLEVIKTGAVVGMQDMG"
BASE COUNT         4665 a         2536 c         2352 g         4892 t
ORIGIN      
        1 tacttctcca ttttttgcat ctttcaaaaa accgctaagc gtaaattttt caattttttc
       61 atcttgagta tatccagcaa gtgggcacag aaaaagcaag cagtaaagaa ttatagtgta
      121 ttgagtcatt aatctattga ttggctaaaa ataagcatat gcctgcataa atcatttatt
      181 ctttaattat tttttttatg aatacacctt tatctgtagt tatttgcaac aaatacatgc
      241 cagtgcttaa ataagaaaaa tcaagcatat tatttgttgg tgtataggct gaatatacca
      301 tttgtcctac gctattcatc aattgaatgg attgcaagtt ggcatttcca ttacattgta
      361 cagcaagtat attttgaaat ggatttggga aaacatggat atcatcaata atagattttg
      421 tagagacaaa tgttggattc gctaataaat catacgcttt tttaaaatca ggaattccgt
      481 atcctattcg tttgtcaggc gttaggtatt gtgatgcact ttgttcgata gctgttttaa
      541 tttcccaatt tgttttagta ggaaaagcct gccataaaca agcggctaag cctgctaatt
      601 gtggtgtagc ataagaggtg ccaccgccag aagatacatt gcccgcatca ttcaaatatt
      661 ttgccgacac gcccatcgct gctacattag gttttacgcg cgcatcattg ggcaagccat
      721 aagcactaga accagcaaca acaccattga tatctacaga cgcaatacaa aatgcactat
      781 cggcatctga tggtgtagaa atatatttcc atggtttaag accttcattg cccgcagcca
      841 cacatactaa gattcctttg ctcgatgcca tatttacggc tttagcagct ggtgtgctat
      901 tattacgcag gtctgtcgta tcatggtttt ccgatgcaac gtcaaacgta gtataaccta
      961 aagaaatact gacgacatcg acacctaatt gattacaacg ttctagagca ctagccaaat
     1021 tgaactcttc ttgcaggcgt tctgaattgt tattttctgt ttgaaaaaga tagtatgatg
     1081 attttgttgc cgtgccaata tattggctat ctttcattcc agcaataaag gaaaagcagt
     1141 tggtgccgtg ttttccgttt gtatcatcat gtacagttga gtcattattt acaaaatcgt
     1201 atgtacctaa aagtcgatta tctgccatga ccgctgcata agcaggattg ttataaacgt
     1261 tataaaatcc gttatcacaa acggcaatcg tcatattctc gccattaaaa ccttgctcat
     1321 gcaaatattc gccatgtaac aaattcactt gtggaaagga agatgtatat tgatcttcaa
     1381 atgctaattc atcttcatta ccaacacaag catcttgcgt tgctactgac attgtatttt
     1441 tatgcgattt tgccgtttca atttttacaa ttgatgcaat aaaaggaaat tgtgctattg
     1501 ttgctaattt tttatcctca atacgaatta atgctgcatt taaccaattt gtagtatgca
     1561 gaattatggc attgttttgt tttaactgtt cgatatagtt agcattaaca ggataatcgc
     1621 gttcgtcgaa tggaatatta aacctattgc gttgttggat tgctttcgta ttaaacaaag
     1681 cttttacatt gatatcaaaa gttttatctt taaaatagat aatatattta gacgtattga
     1741 tttttgcctg tacagaaaat acagccataa aagacaatat gattaggaat ttactctttg
     1801 ccataatcga ttgcgtattg tcgtaaaata aatccttttt ctgctctgtc ttcccatgga
     1861 gcgccaatat ttatttgacc ttccaccagc caaaattctt tatacaccat tccaatactg
     1921 cgagcatatc tttctgccga ataggttttg gtaatcgctg tagaatcttg aatatgtgta
     1981 atcagcaagg ttgaatcaaa tattcgagtg ccattattgt aataggcatc tcgtgctttt
     2041 atggtgtatt tccaatcgtc aagaaaataa ttaaccgtat ctaaaacaaa aggtggtggc
     2101 acggtgataa atttattacc taaccacttt aatccgacgt catttgggaa aaccattttt
     2161 atataacgta gattatcttc gacgcgctcc gctacaagat ttgtttttac taagtaatct
     2221 acattaaaaa tttcccagtt tgttgtaagc gagtctgcat aatagcgtaa tattttattg
     2281 gcagttctac ctaaattatc tacaaactgc tctcctattt tttcttttaa atacacagta
     2341 ttgtagcgtc ttgaattatc aaaatcatta aacaacacag agtccacttt ataaatgaca
     2401 aagttgccat attcgtttgg gaaataatca taaccaatat catatttgct aacatctgtt
     2461 tcttttttac aagatattat agcaataagt gtaaagcaaa aaaaaaggaa tatggtaaat
     2521 ttatatttca ctaataaaaa aaattaattg cagtaaacat tttattctcg cccatattct
     2581 actgcatatt gtttcagaat aaatccgaaa acagcattgt tttcatacgt ctgattattt
     2641 acaagctgtg catctaatcg ccataactct ttatatacca ttccaatatt ttttgcatac
     2701 ttctctactg aatatacttt ttggacatta gaagaatcat taacttgcaa aaccgtcata
     2761 gtcgaatctg tgattatgct accaaatgta taattttgat tgacttgttt gacttcgtaa
     2821 ttccaatcaa aattggtagc cgtaaatttc aaatcggtat agggaaaatg attgagctcg
     2881 ttgtatttat taccacgcca aatatttccg acagagacag gaaatgtcac ttttatataa
     2941 cgaatattat cttccactct ttctgcattc gtcgtatttt ttacaatgta ccaaacatta
     3001 tgtggcaccc aagaatgatt taaactatct gttacgaaac gttcaacttt tttggctcta
     3061 cgacccaaat tgtcaataaa ttgttctgta ataatttctt ttagataatg tgatgaagtt
     3121 cttttaagat tcaatggatc aaaattatta tacaacacag aatctacttt gtatatgaca
     3181 taagtgcccg aatcatcagg aaaataattg tacccaaaat caaacgtgac tgtttctgtc
     3241 tcttttttac aagaaacaaa caacaacact aagactaaga aagaaagtgg tattttcttc
     3301 attgacatca tgcataaata tacaaataag atgtaaattt tgtttaaata gttggtactc
     3361 ttgctcgtct tttttggttt taatgaagga aaatgacagg aatttacttt tacagcatat
     3421 aggaaaaata atttagcgct atttatcaag tcatctgatg tcaggacagt aatttctatt
     3481 tgtataaaac cttcttgttt tgtataacaa ttttatcaaa ttttatgata gaattctatt
     3541 aaacaaatta gcgagccatt ctgtatattt gctaaaaaaa atgaataatc agcaaactgt
     3601 tggaacagcg tttaagacga tttccatatt tcatattgcc atgtgtgttg gattaattgc
     3661 cattgtagct gtttttagat ttattgtaaa acaagataat acagcatcaa caccaaatca
     3721 aacgatggaa atcataggta ttgccgttgg atttatcggc atcatctctg cacgattgct
     3781 tttttttaag caagtaaaaa cggcacatac tgtatctgca ttaaaagaca aaattgctat
     3841 tttcagaaat gcttttatta tacaaatggc catattagaa gccgctgcat tgctcaatgc
     3901 tgttttatat ttcataacaa aaaatgacat gcattttttt attgccgtag gcatcatttt
     3961 attgatgata gtaagacggc caacgcgcgc catggctgga atgaccttat ttaccagcat
     4021 ggaagataag caagttattt ataatgatag cacgcctttg taattacagt aaggcatttt
     4081 tactaattag aatgccatct tcatcgcaat aaataaattg atttggatga aaaatgacat
     4141 gtgcaaagtc aactataata ttttttaatc ctggatattt ttttacagat ttcgttgggt
     4201 ttgtatttag tgctttaatg ccaatttgca tagtgttaat gactgcactg tctctaatac
     4261 aaccattaac aataatgcct tcccattcat ttttgatagc tgctgccgca attaaatcgc
     4321 ccactaaagc gcaacgcaag gaagcaccgc catcgataac cagcactttg ccttttccat
     4381 tttcattgcg caaggtttcg ccaacaggcg tattgtcttc gaaacacttt atcgtataaa
     4441 tttgaccatg aaattgtttt ttaagtccat aattttgaaa aattgacatg gcgacatgca
     4501 cctttccttc gttagcatca tacaaatctg cggtggaaaa tgacataaaa agatttttaa
     4561 gttgagaaat atgcctttaa attacaaatt agatttaaaa atcataaaac ttgcttcata
     4621 aatattacat ttactatatg gcaaaacaat atacttcttc attaatgatg gttcgccctg
     4681 caagttttca gttcaatatt gaaacagcac aatcgaatgc ttttcagcaa cacttagaag
     4741 gtttaagtaa ggaagaaata aaatgcaaag ccatcgaaga atttgatgca tatgtagaga
     4801 aattacgcga acataaaatt gatgttacag tcattcaaga cacaccaaat ccagccaagc
     4861 cagatgctat ttttccaaac aattggataa gcatgcatgc tgatggcaca atctatttat
     4921 ttccaatgtg cacagcaaac agaagattgg aaataaggcc agaaatcatc aaagaaattg
     4981 aaaaaaaatt tgtggttaat aaggttgata acatgagtgc tttggcaaac gataagctat
     5041 ttttagaagg cactggtagt attattttcg accatattca tcgcaaggcg tatgcgtgta
     5101 tttcgccaag aacagacaaa aaattattgg aagaattttg tttaaaaata ggatatcaag
     5161 caattacatt tacaagtgtt gaccttcaga acaagcttgt ttatcacacg aatgtgatgt
     5221 taaccattgg cgatacattt gcaattatct gcctggaaag cataagagat gaaagagaaa
     5281 gagaattagt aaaacaacat ttagcaaaca gtggacatga catcattgac attacatttg
     5341 agcaaatgca gtcgtttgca ggcaatatgt tacaagtaca aaatactgat ggcaaaacgt
     5401 atgtagtgat gtcggaaact gcattagaca gtttaactgc aacgcaaata aaacaaatag
     5461 aaatgcatac tgcaattttg tctgttgaaa ttccaacgat agaaactata ggtggcggaa
     5521 gtgctcgctg tatgttggca gaaatatttt taaaagaaaa aaattagcgc gataaaatcg
     5581 atttccattt atcgctttcc gcaaattctt taatgacttg attgcgttgt tgcaataatt
     5641 ttatcatgta ttttttcacg taaagcatat cagcaattat gccaaaaata ccaaatggtg
     5701 atttgtattc aaagatatct atcaacacta cttttccatt tttttcttca aaaatatggt
     5761 cgtggtgata aaaatcaaag atgccttttg tttgtgaatc tctgaaaata aaaggtcgtt
     5821 taaaattaga aattttagaa gaaagatatt gtgtaacacc taagtgttta gcttgccatg
     5881 ttacagtctc gccatcttcg ataagtccag aagtgcggcc agcaacagct tcttcatttg
     5941 tatgtttcat ggaatattta tgcaagtcaa tacttctcga caagtcaaaa caaatgtcta
     6001 tcggagcagc aatttctgtg gttaattgaa taactggcat aaacttatgt acttaaatat
     6061 acgacataaa aagtattgaa ccaataaaaa acacaaggtt ttaatgataa ataaattcgc
     6121 ctttatcaga agatatcgtt acttgttttt tatcagctgc ttcgacgtaa ccaattactt
     6181 gcgcttcgac agcaaatgaa ttggcaattg aaatgatgat ttccgcatgt tctggtgcgc
     6241 aataaatttc caatctatgg ccacaattaa acacttgata catttctttc cagtctgttt
     6301 tactttcttc ttgtattaat gcaaataatg gtggaacagc taataaatta tttttcacta
     6361 tgtgtaaaca atcgacataa tgtaatattt tagtttgcgc accaccactg caatgtataa
     6421 taccatttat attttttacg cctaattcat ctataacacg tttcacgatt ggcgcatacg
     6481 tacgcgttgg cgaaagcact aattttccgg catctaatgg actattttcc actttatctg
     6541 tcaacaattt tgtgccagca taaatgaaat ccatattggt atttgggtcg aatgcttctg
     6601 gatatttctg tgcatatatt tttgagaaaa catcatgtcg tgcagacgtt aaaccattgc
     6661 ttcccatacc accattatac tcattttcat aagatgcttg gccataagat gccaagccaa
     6721 caatcacgtt gcctggtttt atattagcat tatcaatcac gtctgcgcgt ttaaatcgtg
     6781 cagtcataac agcatcgaca gaaacggtgc gaatcacatc gcccatgtcg gcagtttcac
     6841 cgcccatagc atgaatattc acgccatttt cgtgcatcat ttctacaact tcatttgtgc
     6901 catcgataag tgctttaatc acttctccag gaatcagatg tttattgcgg ttgatgatac
     6961 ttgacattaa aaagttagac gttgcgccca cacaaatcaa atcgtctgta ttcatgacaa
     7021 tggcatcttg cgctatgcct ttccacacag ataaatcacc agtttctttc caatataaat
     7081 aggctaacac acttttcgtg cctgctccat cagcatgaat aatagagcaa tagttttcat
     7141 ctagtgtcaa atagtctgga aatattttac aaaatgcatt tggaaataat cctttatctg
     7201 cattttttat agcggcatgt acttcatctt ttgtggcgga aacaccgcgt tggtcgtatc
     7261 tgttggtatt ggacatttta aaatttttag actccaaatt gttctaaatg atgatttaag
     7321 tgtatataca tggcatcgcc ccaaagttct ttgctcatgg tgccaaatat tgggtggcta
     7381 ccattaaaag aagatgcttg aagaaatgct tggtaagttt caagaaatga ttttttggca
     7441 ggctcgaaat cgatatcctc ttttaccaaa aatgcttgtg cagtcatcat attttttggc
     7501 catttcgtca ttgcctttat catcatccat cgtgctattg gattggcaac tacataatat
     7561 aagggttcgc gaggtacttg tattttttcg ataggcacgg caaatgctgt tgtcatgtgt
     7621 tttagcattt gtgacacgct catttttccc catttgcgtt gtgaagaagc agataaattg
     7681 ttaatgcgtt gcattatctc tgattgcgtg gaagcgttaa atagattagg catactttaa
     7741 ttatgctaca aaaataagct atttttcctt agtgtaagga catgattttt ttaaataaat
     7801 gctaatgaaa gtgaactcga attaaactat tattgaatag gagcaaatta ttcactcact
     7861 attttaaaag ttgttcttct gtttttttga tgttgttctt ccgtgcaggt ttcacaatcg
     7921 catttttcta ttggcattgt ttcgccatag ccttttgccg tcagcctatt ggcatcaatg
     7981 ccctttccta ttagatattt cactacgctt tccgcacgtt tttgcgagag tgtttcattg
     8041 tatgcatctt ttcctctaca gtctgtatgt gatgctaatt caattttaat atttgggttt
     8101 ttttctaaca catcgacgag caaagtcaag ctttctgttg cgtcagcacg aatgtcccat
     8161 ttatcataat cgtaataaat attttctaaa acaatttcaa catttttata aatttttgac
     8221 aaaatgatat tgcgttcaat gattaccgta tcgccatctt ttgcctcatt atttgcaaat
     8281 gtgcttactg catctacttg cgacaaatag ccgtctttag taatattata agtaaattct
     8341 acatttgctg gtattatcga ttgaattttt ccaacagaat tagttttcat tttatcgact
     8401 ttaggtaagc tgatgatggc attttcgaca ggaatttgtc ccaataattt tgatgatgga
     8461 ttatttggat tttcataatt attttcttgc acatgcacat tcaatacata cactgttggc
     8521 ggcaatggtt ctttgggtgg cacacgtttt acaaaataat aaatatcgtc tttgcctttg
     8581 cctccacttc gattagacac caaataacca ttgagcaaga cagaatcttc tattacctta
     8641 gttaattgca aacttaaatc atctgctcct gagttgacac caaatcctaa attttgtaca
     8701 ttggtataaa ttttaccttc ctttgtagca gcaaaaatat ccaatcctcc atatccaatt
     8761 ttactatttg atgaaaaata caaagtatta tcattggcaa tgacaggaaa tagctcatct
     8821 ccttgtccat taatttttga acctgcattg attggatttt catattcgcc agcaatattt
     8881 ttcttggcga aataaatatc tcgtccgcca taaccatctt tgttatcaga agaaaaatac
     8941 aattcatttt catcagttga caaatacgct tgcccttcat ttatggtatc ggcaaaaagc
     9001 ttcacacgtt gtggtgtact ccatgagtcg tcaatatttt tatcaactcg gtagatatga
     9061 caatattcat ctacgttttt attagcgcta ccacatctag taaaaaaggc agttttacca
     9121 tttttagtaa aaataatatt agcatcgtga taaggcgaat tgaatggagt accaatattt
     9181 acaggcgaat tgaatgcaat atcgtttaat ttttgtgaac tatatatgtc tgcatatcct
     9241 tctccagtcc aatcatcagt attatttttg cctccacctc tcgtcgatga aaaatagagt
     9301 atattatctt tcaaaaatgg tgagaagtca atgctttctg tattaatctc taaattgctt
     9361 aattttgtat gctgctttgc tttagtttgc tttacagctg cttgtaaaaa atcgtattct
     9421 ttttgtaagc ggaatttctc tgttttattg gctttaagat aatcttctaa tgtttttagt
     9481 gcttctgtat atttctcatt tcgtttgagt gcattaacat aaagcatcaa cgaattatca
     9541 agcacacgcg cgtctgcaat ttttttgtac catgtttctg ctgctatgta attaagttgc
     9601 ttgtcataac tctgtgcaat ttttaaagca atttttgatt gttgagcaat atcttttgtt
     9661 ttgctataat ctttttgaag catttctgct gccagtgtgt atttttttat tttaaatgct
     9721 gtttcgccat cttgcacttt tgaagatgac ttgcatgatg caagcacaag gacgatacca
     9781 atacataaaa tgttacaaat tcttttcatg cgcaataagt tctaactaca acaaatatac
     9841 ttgcttgaag tttatttttt gaatatgcta ttaaatattt cgagaataaa tgaattattc
     9901 tgatgtcata cgtattcaaa gaaattacaa aagcatgtaa ataaaaaacc tgcctaaatc
     9961 ggcaggtttt tctgtacttc taaaaaatat tatttagtaa ataacatctc acgatatttt
    10021 ggcaatggcc ataattcatc atcaatgata gtttctaagt tgtcagtaac atctctgatt
    10081 tcatccatga ttggtcttac tttatcagaa tagatttgtg cttgtttaag cgcattgctc
    10141 aaatcattgg cttttacacg taaatcgagc atatcttttt ctaattttat tactttctca
    10201 atgccttcag aaatttgttt taacaaactt gattgtgcac tgtatgattt agcatcgatg
    10261 ccaatttctt tcaagcctct gatattggta attaatttat tttgatactc taaacatgca
    10321 ggaattactt gattgataac gatttcacct aaagcacgag attcgatttg gatagcttta
    10381 atatagtttt ccaatgcaat ttcataacgt gcttccactt ctctatgtga gtagatacct
    10441 aaatctgcaa ataatttaac tgaagaatca gaaacgaaag cacctaaggc tacaggcgcc
    10501 gttttgttat tgcttaagcc acgttttttc gcctctttat gccaatcatc tgaataatta
    10561 tcgccttcaa acatgatacg ttttgcatct ttaattgttt ttctaagcac ttttaaaata
    10621 gcaacgtctt tatagtcacc acctttgata tactcatcta cttctacttt aaacaacttc
    10681 aattgctcag caacgatagc atttaaaacg gtcattggca atgcacaatt ggcagaagaa
    10741 ccaaccgcac ggaattcgaa tttgttacca gtaaacgcga atggtgaagt tctatttctg
    10801 tcagtattat ccaacatgat atctggaata cggttgtgaa tatctaagcg caaagtgata
    10861 ttatcttgct catcaaattt atcttcatcg acacgtgatt caatcatttc caatacttct
    10921 tttaagtaag aacctgtaaa tacagaaatg atagctggtg gtgcttcgtt agcacctagg
    10981 cgatgctcat taccatgcgt tgctactgaa gcacgtagta attcttcgtg tctgttaact
    11041 gctgcaatag tgtttataaa gaatgtcaag aattgtaagt ttgttcttgg cgtttttcct
    11101 ggagataata agttgacacc agtgtctgtg cccatactcc agttgttatg tttaccactt
    11161 ccatttattc ctgcaaatgg tttttcgtgg aataatactt taagtttgtg cttactagaa
    11221 actctgtcca tcacgtccat caataatgaa ttatgatcaa cggctaagtt agcttcttca
    11281 aacattggag cacattcgta ttgtgaagga gctacttcat tatgtcttgt gcgaacagga
    11341 atacctaatt ttaaacattc gatttcaaaa tcacgcatat aggcatatac acgctcagga
    11401 atggcaccaa aatagtgatc ttctaattgc tgaccttttg gagagcgtct gcctagcaat
    11461 gttctacctg tcatgactaa atctggacgt gcgtagtaca tcgctgaatc tactaagaaa
    11521 tattcttgtt cccagcctaa tgtagtatat acatgtttaa catctctgtc gaaatagtgt
    11581 gctacttccg tcgcggcttg gtctaatgct tggattgatt ttaataaagg tgctttatag
    11641 tctaatgttt cacctgtata tgagatgaaa atagtaggaa cacacaaagt ttttccgctt
    11701 ccaacagtca taataaatgc tggcgaagta acgtcccaag cagtataacc tctcgcttca
    11761 aaagttacac gaataccacc tgatggaaat gaagaggcat ctggttcttg ctgtacgagt
    11821 gcatctgaac taaattcttc aataccactg ccacctttag gcataaaaaa cgagtcatgt
    11881 ttttctgcag ttgtgcctgt taatggttga aaccaatgtg tgaaatgtgt tgcgccttgt
    11941 ttcatcgccc aagctttcat tgctgacgct acctgatccg caatttctct atctactttt
    12001 ttgccgtctt ttatcgacgc tgctaatttt ttatatgcat ctgaaggtaa aaattctctc
    12061 atttccttct cgccaaatac agcagaagca aaaaaatctg aaattttttc tgatggtaat
    12121 tctacattta cttttggtct tgaagaaaat gcagataatg cgttaaatct tgtttttgac
    12181 atacctaata tatttgtatg ctaatatcaa taaaatttaa cagaaataag agaaattaaa
    12241 gttaagaaat gaaaaattta gctattattt agcactgtct gcttattttc ttgtctcacc
    12301 aaacacaatc ctatcaaaat aaatggtatt gcaattgacc gataacgtac tattgcacca
    12361 gaattgttta ctattaatcc tattaagaat agcgtgctta agccaaataa gatacaaaaa
    12421 agcgcataag gattttttat caccaatttt aggttagatg aaaacagtaa agctactatt
    12481 gtaagtaata gtataatgga ttcgaaagaa gctaaataag ctaagaaata attacaatca
    12541 ttaggcaaag gtcgaataaa tggatttgta aaagcttccc aaagtagaat aagaactcct
    12601 ttgaaattat gtggtaaatg ctctacttga aaagacatat ttcctggtga atttaaaaaa
    12661 tatgttcttc tacttaaaaa ttcatgtgta agactaaaat ttggaataat caaatctaac
    12721 agcaacaaaa caaacaccaa aagcattgcg gacaaagcaa ttacttttaa ataagatgtt
    12781 ttaatttcta atttattata caaggcaatt gaacccatta atggcaataa aaatgccaat
    12841 aaatatatac gcgtataacc taagataaac aaaccagcaa tggaaaataa aatattgcga
    12901 ttagtatgtt ttgctgttaa aacaatatcc atattataca atatgatgcc caatgcaaaa
    12961 ataacaattg attctttatg aattcctgat gtccaaaata caatagaagg aattccaaat
    13021 aaaataaatt gtaccatttt tagatggcgc actttgtctt gaaaaaacaa atagagatta
    13081 tacgttccaa taaatgacaa gaaagcaaaa atgatagcat gtacgttata cacaccaaat
    13141 gaaaatgggc gcaacaatgc atttaatcga acgataaaat aattactact atcaaaccaa
    13201 aaatgcatcg catctgtata tggcagtaaa taagctggaa caggcttgaa atcattgctg
    13261 cctattgcca atttaaaata aataaacgga tctgttttca gcgttttata aataatctca
    13321 ccatcataaa agtataagaa tgtatcattg cctgttaaaa ttccatttct aaaaaaaaaa
    13381 ccaaacaata atgcactaaa cactttcaag gaaaaaccta tatataatac attccgcgaa
    13441 atcgaaggaa caggaaatag ccttttatta cttatcagaa ataagaataa taaaaaataa
    13501 aatgttggcg ctaataattc ttgcaacatg gctatacgac gtaaatttac actttttatt
    13561 gtaatgataa taattattcc aaattggtaa ataagaaatc cgtaattttg agctgaatta
    13621 ttaatatatg tcaactccaa ttcagcaacc acaagtcaac gaagaaatag caaaatcatt
    13681 gggattattg ccagaagaat atcaaaaaat acaagatata ctaggtcgca ttcctacatt
    13741 tacagaatta agtgtttatt ctgtcatgtg gagtgaacat tgcagttata aaaactccat
    13801 aaaatattta aagaaattac cgagaaaagg taaagcatta ttagtagaag ctggagaaga
    13861 aaatgctggt ttagtagata ttggcgatgg ttgggcttgt gctttcaaaa tagaatctca
    13921 taatcatcct agtgccgtag aaccatttca aggtgcagca actggcgttg gtggtatttc
    13981 tcgagacata ttcactatgg gcgctcgacc gatagcatcg ctcaactcat tgcgctttgg
    14041 aaatttagag aataaaagga cacaacactt attaaaaggt gtagtaaaag gcatatcaca
    14101 ttatggcaac tgtttcggcg tacctacagt tgcaggtgaa gtatattttg atgattgtta
    14161 tcaagtgaat cccttggtga acgcgatgag tgttggtata gtaaaagttg gtgaaactat
    14221 ttctgcaact tcagcaggtg tcggcaatcc aatttatatt gttggtgcct ccacaggcaa
    14281 agatggtatt catggtgcta catttgcatc tgaagattta ggggaagatg cggaagataa
    14341 aataccatct gttcaagttg gtgatccatt tacagaaaaa ttacttttag aagcaacttt
    14401 agaagtcatt aaaacaggcg ctgtggtagg catgcaagac atggg
//
LOCUS       DAOQQV010000065        13962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1451_length_13962_cov_9.729489, whole genome shotgun sequence.
ACCESSION   DAOQQV010000065 DAOQQV010000000
VERSION     DAOQQV010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1451_length_13962_cov_9.729489"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLL59_07815"
     CDS             <1..734
                     /locus_tag="PLL59_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMY23753.1"
                     /translation="AFIPLIIINLKSLSLPKEKLVFVPLLGLFGNLIPSIMFCIAGTK
                     IDSALSGIMNSTTPLFALTIGSLLFGISLTKNKIIGVAIGFIGTLIIILSKQSITSIN
                     PYILLPLLATICYGLNANIYKHHFQQEHPIRVALLQYAFVAFFSILYLIISGGFAKII
                     AQPTVSWQALKYLLLLGVLGTALAQVYFNILAQRTTALFATMTTYVIPMVSIIIGIFI
                     GEEILFLHIFGLMVILIGIYIGSRG"
     gene            901..1413
                     /locus_tag="PLL59_07820"
     CDS             901..1413
                     /locus_tag="PLL59_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMY23754.1"
                     /translation="MALILPCREKTPIIGENCYLAPNATIVGDVEMGNDCSVWFNAVI
                     RGDVNSIKIGDKVNIQDGAVLHCTFEKTKLNIGNNVSIGHNALVHGCTLEDNVLVGMG
                     AIVMDNCYVEKNALIAAGAVVLENTRVEAGSIYAGVPAKKVKQVSEEVFKDQNERIAN
                     NYLKYSSWFK"
     gene            complement(1424..2449)
                     /locus_tag="PLL59_07825"
     CDS             complement(1424..2449)
                     /locus_tag="PLL59_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY23755.1"
                     /translation="MILWKKKDFIITALVEKPSCHKLAHVETTKKRILITGAAGFLGS
                     HLCDRFIKEGYHVIGMDNLITGNIKNIEHLFPIKDFEYYHHDVSRFVHVAGKLDYILH
                     FASPASPIDYLKMPIQTLKVGSLGTHNLLGLAKEKNARMLIASTSEVYGDPTVHPQPE
                     EYWGNVNPVGPRGVYDEAKRFQEAITMAYHNFHGVETRIVRIFNTYGPRMRLDDGRAL
                     PAFMSQALNGEDLTMFGDGSQTRSFCYVSDLIDGIYKLLLSDYHLPVNIGNPQEITLL
                     QFAEEILKLIDTKSKIVYLPLPKDDPKQRKPDITKAKNILGWEPKVERAEGLRITLEY
                     FKKELGK"
     gene            complement(2385..3719)
                     /locus_tag="PLL59_07830"
     CDS             complement(2385..3719)
                     /locus_tag="PLL59_07830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HMY23756.1"
                     /translation="MNIAVVGTGYVGLVSGTCLSETGNNVICVDIDANKVAKLQAGNI
                     PVYEPGLDVIFQRNIKEGRLQFTTNLEEAIKDVSLIFLALPTPPGGDGSADLSYVLGV
                     AEKLGMIMQDYKVIINKSTVPVGTAEKTKAAIAKHAKVPFDVVSNPEFLREGVAVDDF
                     MKPDRVVVGTSSERARKLMEELYRPFVRQGNPIIFMDERSSEMTKYAANSYLATRISF
                     MNELANLCEKTGADVETVRIGMGSDARIGKRFLFPGIGYGGSCFPKDVQALAKTADEN
                     QYDFKILKAVMHVNDIQKHVLVEKIINHFGADLTGKKIAIWGLAFKPNTDDIREAPAL
                     YIIEDLLNAGAEIVAYDPEAMPNVQQYFANLSEDKKSRLHFVDSYYLALQDAAFLAIL
                     TEWTEFRTPDFNKVKSLLKQPIIFDGRNLYDLDIMEKEGFYYNSIGRKTIMP"
     gene            complement(3799..5193)
                     /locus_tag="PLL59_07835"
     CDS             complement(3799..5193)
                     /locus_tag="PLL59_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23757.1"
                     /translation="MQLFPQQVENFWLRQDLIFASDFILVPLYLYFIHVQLKKYIAKK
                     PEHYKEYMMKAWYVRLAGCILTALMYDYYYNGGDTTTFYFYALGVIKMLFTDPEMIPQ
                     IMLNPKSFYSLRYLSESKYFIESGLYFYDGTTAIIIWLTSACLVVFLQSYMLTSITFT
                     LFALYGCWKIFSMFYDMYPHLKKQMAISCLFIPSVFFWGSGVMKEPICIGGLGMMSYY
                     MYHFFFKKGNKLKSLFLALLGAFIVFSIKVYIILAFLPALAVWVFARYRYNIKSPFLK
                     TISTPLFIVLGLGAGVVVLQSMASYAERYAFEEIMRTAKDTQNWLVYSSKEQNGSFYT
                     LGDIEYSTMGMLKVFPKAINVSLFRPYIWEIRKPILLAASLEGIVTLYLTITLLFKTG
                     FVRFLKLIASNPEVQFCLVFSIIFGFAVGFTSFNFGALARYKIPFMPFYYIALFILSD
                     AQNKTQNTTAKTKK"
     gene            5358..6275
                     /locus_tag="PLL59_07840"
     CDS             5358..6275
                     /locus_tag="PLL59_07840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY23758.1"
                     /translation="MLLSVVAPIYFAQNSVEVLVKLIVSECSLLQMDFEIILIEDGST
                     DNTWEKIEVLSSDIPQLRGIKLSRNFGQHYAIACGIEQAKGDWIIVMDGDLQDHPSAI
                     KQLLAKANEGYDIVVAKRISRQTSFLNKIYSIIFWKILSYLTGRPLHHSISNFGIYHR
                     RVIDAIISMRDAIRFFPTMVDWVGFKKTTIDMPQQARHEGKSTYSFNKMIRLALDVVL
                     VNSEKPLRLVTKTGIFISIISFVVGIIYLYKHLTHQIEVPGYASIIISIWFLGGLIIC
                     ILGILGLYIGKTFDAVKNRPYYIIEKTTR"
     gene            6275..6994
                     /locus_tag="PLL59_07845"
     CDS             6275..6994
                     /locus_tag="PLL59_07845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY23759.1"
                     /translation="MEIIQALTWDSDFFQLRIGKIDELSKLQSITAAAINLFDLIYIE
                     QTAQNLDTFLENQTNFNITYTDIKTIYRKQLLHNVTSVDKQITVYPFATTNDALINLS
                     IQAGAFSRFKLDPNFGATSYKNLYRQWIENAVQKVSADIVLVYGDIQYPKGFITLVFK
                     ENLAKVGLIAVDQQHQNEGIGKALMEAAEYFAIKNNFTTLEIITQTENKNACAFYERI
                     GCHQTNILYTHHHWKKHANTI"
     gene            6978..8114
                     /gene="rffA"
                     /locus_tag="PLL59_07850"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             6978..8114
                     /gene="rffA"
                     /locus_tag="PLL59_07850"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HMY23760.1"
                     /translation="MQIPFNKPYLTGKEITYIQEAAAIGKLSGDGVFTKKCERFFEEK
                     YGFKKSMLTSSCTDALEMAAILINIQEGDEVIMPSFTFVSTANAFIMRGAKIVFADSS
                     AEHPNIDATKIANLITRNTKAIVVVHYAGVACDMESIMALANQYNLFVIEDAAQAIDA
                     YYKGKPLGSIGHLAAFSFHETKNIIAGEGGMLIINDEKFIERAEIIREKGTNRNKFYR
                     GEIDKYNWVDIGSSFLPSEITAAFLWAQIEAIDDIQQKRISIWNTYYEALLAYADFFQ
                     LPVIHEYASNNAHAFYLVCQQAEDRNQLIDFFRSKNILAVFHYQSLHKSPYFIDKHDA
                     RILQEADRYSQCLLRLPLFYELSTEEITHTIIPTITTFVEQYAY"
     gene            8104..9609
                     /locus_tag="PLL59_07855"
     CDS             8104..9609
                     /locus_tag="PLL59_07855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMY23761.1"
                     /translation="MPINKKNILIAFFGVIGFAFMINTINADFVKNASFLAENERLVH
                     GQTIYSIDNSFYLPQIKNWLAGHGFTCDASDKKYLVRRTPVYPMFYGVHYLLFGEKNS
                     FFFIKISQTILFALSAVLFLLAVNNLTSNKKMAWLAFLLYGFNPTLISNTYFTLTESI
                     SPALVVLTLYFFSKAMRNSKLRDWLTTGIFFSIASLCRPPIFFFGGAVAIAILLLYYK
                     NKTSLLKTTFAFGVGVLLVFTPYVIRNYKVTNGEFILLEKYYGDPMTYGLQNIELRKW
                     ISTWTNPADYSAESVSNKMWQQILSKRDKQTLIDSMLIALPKAAFTHYSKNTVASIYN
                     CVYDFYAYKFTSPTKKDLAIAEQNAVLFIKKINDKHIAQHPFSYYVIMPMKMMKSIIF
                     QSNAGTLYFLYDYKNNYLFFVIKAILLLLNILLFISLIGNILFIKKHLIIYIAILFFV
                     SINCFYMLFVINYFEARYLVPMFPFLYISGAIFAVEMYEFMKKKITLLNNA"
     gene            complement(9587..10246)
                     /locus_tag="PLL59_07860"
     CDS             complement(9587..10246)
                     /locus_tag="PLL59_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23762.1"
                     /translation="MIATQCIDNSIPFVRLTDTVGFALDLMQEFKTPALAVLNKEIYC
                     GLITENFLLELDDTLLIDALNEHLLTYKISSDLHLFDVIKSSAEFDSFFLPVVNATNE
                     YLGISTPQKILAAFSQNTALNNSGGILVLEMDTKNYSLTEISKIVESNNAMILHSILV
                     STSQANQIQVSLKINKNDLKDIQLSFERFQYNVVAVFHQSEYEHQLQERYESLMRYLE
                     V"
     gene            complement(10291..12768)
                     /locus_tag="PLL59_07865"
     CDS             complement(10291..12768)
                     /locus_tag="PLL59_07865"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMY23763.1"
                     /translation="MKAVKANILILFFLGYFSVHAQTFIDGIVLDSVSKSPISYANIY
                     IKGTTIGVTTDDTGYFKLPLTQAFDSITAAYLGYEEKTIRLSMQSKQRLVFLLSSSAN
                     TLAETVIVANKESLEDYLIRKILENKEKNDKKHLQNYSYELYNKIELEVKNMSDKFMN
                     RKILKPFKFVFDNIDSTSEEEPFLPLMLSENISDYYYTNKLNKKREIIKASKMSGISD
                     ASFNEFLNATYQDINVYDNAYTILEKQFISPIANVCKTFYKYKVIDTLVLDNIVHYKM
                     LFEPKTKGDNTFLGSFIVSENNYAIKSIQLRMAAHVNINFVKKIEINQDFDNVDGLCW
                     MMTKDHLVIEFKPLEKTPAIVTRKNTVYRNFKINTAYNDSMIAVLKEELTMDEHTIKK
                     DQAFWDTSRFIQLSKNEAAVYHIIDTLKTVPAYKTYVDIINFVLTGYYKAGPIEIGPI
                     LNTVSFNKLEGWRFGIGLRTNDKMCSWAQISAHIAYGLRDKKVKGDVGVRFLVDKRPR
                     QIISASYTRDVSELSKTSDLLAADNILSIIINRRKPDVRLLYFEEAKLGYSIEYKQGM
                     SFGIAFQNRYIKQGLVPFRYALTNKELVTDTLHSLRQSELIINARFAIGEKYINKNSL
                     FRSSINTSKVPVIQFEYVYGINKFLGSQFNYHKLTLSYAQIIPIRTIGRFEMHIQAGK
                     VFGTVPFLLNEVHDGNQTYAFSNTAFNVMNDYEFYSDRYLQWNFSHHFDGFFLNRIPG
                     IRKLKLREVIHTRGIWGNASAKNMAFNQLNTSFIKPIGKIPYIEVGFGLENILKFIRW
                     DVMWRVTHRDNPKAYKWMMTFGFNFNF"
     gene            12814..13596
                     /locus_tag="PLL59_07870"
     CDS             12814..13596
                     /locus_tag="PLL59_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HMY23764.1"
                     /translation="MKSLEIISYNVNGIRSAVSKGLIDWMKTTNHDVYLFQEIKANEL
                     DIPSEHFHEIGFQTHWFSAQKKGYSGVGIAIKNEVLHDNVVKGMDIHQYDFEGRLIRL
                     DIGDITIINSYFPSGSSGDERQQVKMAYLDDMYKYIHDIKQDSQKIILAGDYNICHTE
                     IDIHDPKGNKNSSGFLPEEREWMSKLFNSGFIDTFRHVHPSLLHHYSWWSNRANARGN
                     NKGWRIDYLATTKEMEQQIEDAKIFMDVKHSDHCPIYLKISI"
BASE COUNT         4508 a         2413 c         2350 g         4691 t
ORIGIN      
        1 ttgcttttat accattaata attatcaact taaaatcatt atcgctgccc aaagaaaaat
       61 tagtatttgt acctttattg ggtttgtttg gcaatctcat tccttcgatt atgttttgta
      121 ttgctggcac aaaaatagac agcgcattgt ctggcatcat gaattccact acgccattat
      181 ttgcgctaac aattggtagt ttgttatttg gcatttcctt aacaaaaaac aaaattattg
      241 gtgtagccat tggttttatt ggtactttga ttattatcct atccaaacaa agcattactt
      301 caatcaatcc ttatatttta ttgcctttgc tcgcaaccat ttgttatggt ttaaatgcca
      361 atatttacaa acatcatttt caacaggagc atcccattag agtagcgctt ttgcaatatg
      421 ctttcgttgc atttttttct attttgtatt taattatttc aggaggattt gcaaaaatta
      481 tagcacagcc tacagtaagc tggcaagcgt tgaaatattt gcttttatta ggtgttttag
      541 gcactgctct tgcgcaagta tattttaata ttttggcaca gcgaaccacg gcattatttg
      601 ccaccatgac aacttatgtc ataccaatgg tgtcgataat cattggaata tttattggtg
      661 aagaaatctt attcttacac atttttggtt taatggttat tcttattgga atatatattg
      721 gatcaagagg ctaatgtttc ttagttgtac caaaggttgg acattggcat ttctttgctt
      781 ttgctgttgc acaagattgc aagagcatta ttgataaaat tatcacgatt atcgtgtaat
      841 ttagtttcga ttttttaaat acttttatca ttatctaaac aagtataaaa ttataaaaaa
      901 atggcattaa tacttccatg cagagaaaaa acgccaataa ttggtgaaaa ttgttattta
      961 gcaccaaatg ctactatcgt tggcgatgta gaaatgggca atgattgtag tgtgtggttc
     1021 aatgctgtaa tcagaggtga tgtcaattcc ataaaaattg gcgacaaggt taatatacaa
     1081 gatggcgcag tcttacattg cacgtttgaa aaaacaaaat tgaatatcgg caataacgtt
     1141 tcaattggtc ataatgcatt ggtacatggt tgcacactgg aagacaatgt gctggtaggc
     1201 atgggtgcca ttgtcatgga caattgctat gttgaaaaaa atgccttaat tgctgctggc
     1261 gcagtcgtat tagaaaacac aagagtagaa gccggtagca tctatgccgg cgtgcctgcg
     1321 aaaaaggtaa aacaagtttc tgaagaagtg tttaaagacc aaaacgagcg aattgctaat
     1381 aattatttga agtattcgag ttggtttaaa tagaatatat aatttatttt cccaattctt
     1441 ttttaaaata ttccaaggta attctcaaac cttccgcacg ttctactttc ggttcccaac
     1501 ctaatatatt tttcgcttta gtgatatctg gtttgcgttg ttttgggtca tcttttggca
     1561 aaggtaaata tacaattttc gattttgtat caatcaactt caaaatctct tcggcaaact
     1621 gcaatagcgt aatctcttgc ggattgccaa tatttactgg caaatgataa tcgctcagta
     1681 atagcttata aataccgtct atcaaatcag acacgtaaca aaaagaacgt gtttgtgagc
     1741 catcgccaaa catagtcaaa tcttcgccat tgagcgcttg gctcataaat gctggtaaag
     1801 cacgaccatc atctaagcgc attcgtggtc cataggtatt gaagatgcgc acaatacgtg
     1861 tttcaacgcc atgaaaatta tgataggcca ttgtgatagc ttcctgaaat cgttttgctt
     1921 cgtcatatac acctcgagga cccactggat ttacgtttcc ccaatattct tctggctgag
     1981 gatgcacagt tgggtcgcca tatacttctg atgtagatgc gatgagcatt ctcgcatttt
     2041 tttccttagc caaacccaat aaattatgtg tccctaatga gcctactttc aatgtctgaa
     2101 ttggcatttt cagataatct attggcgatg caggtgaagc aaagtgtaaa atataatcaa
     2161 gttttcctgc cacatgcaca aatctgctga catcatggtg ataatattca aaatctttta
     2221 ttggaaaaag atgttcaata tttttaatat ttccagttat taggttatcc atgccaataa
     2281 catgatatcc ttctttaata aacctatcgc atagatgaga gcctaaaaat ccggctgcgc
     2341 ctgtaattaa tattcgtttc tttgttgttt ccacatgtgc taatttatgg catgatggtt
     2401 tttcgaccaa tgctgttata ataaaatcct tctttttcca taatatcaag atcgtataaa
     2461 ttgcgaccat caaaaattat aggctgtttt agtagcgatt ttactttatt gaaatctggt
     2521 gttctaaact ctgtccattc tgtcaaaatt gctaagaatg ctgcatcttg caaagcaagg
     2581 taatagctat ctacaaagtg taatctcgat tttttatctt ccgaaaggtt ggcaaaatat
     2641 tgctgaacgt tcggcatggc ttcagggtcg taagcaacaa tttctgcacc agcattcagc
     2701 aaatcttcga ttatatataa tgctggcgct tctctgatat catcggtatt tggcttaaat
     2761 gctaagcccc aaattgctat tttttttcct gtaagatctg caccaaaatg attgataatt
     2821 ttctctacca atacatgttt ttgtatgtca tttacatgca ttactgcttt tagaatttta
     2881 aaatcatatt gattttcatc tgctgttttt gccaatgctt gaacatcttt tggaaaacaa
     2941 cttccgccat atccgatacc aggaaataag aaacgttttc caattcgcgc atcactaccc
     3001 attcctattc gcacggtttc tacatcggct cctgttttct cgcacagatt cgccaattca
     3061 ttcataaagg aaatccgtgt tgccaaatac gaattagctg catattttgt catctcagag
     3121 ctgcgctcat ccataaaaat aattgggttg ccttgtctaa caaaaggacg atatagttct
     3181 tccattagct tacgtgctct ttccgaagaa gtgccgacga ctacgcggtc tggtttcata
     3241 aaatcatcaa cggcaacacc ttcgcgtaga aattcaggat ttgacacaac atcaaaaggc
     3301 actttcgcat gttttgctat agctgctttt gttttctcgg ccgttcctac tggtaccgta
     3361 cttttattga taatgacttt gtaatcctgc atgatcatgc ctagtttttc agcaacacca
     3421 aggacataag acaaatcagc ggaaccgtct ccacctggtg gcgttggcaa cgctaagaaa
     3481 attagtgaaa catctttaat ggcttcttct aaattagtcg tgaactgcaa cctgccttct
     3541 ttgatattac gttggaaaat cacgtctaga cctggctcat aaacaggaat atttcctgcc
     3601 tgcaattttg caactttatt tgcatctata tcgacgcaaa ttacattatt tcctgtctct
     3661 gacaaacaag tgcctgacac caatcctaca taacccgtac ctactacagc aatattcatt
     3721 tttaaaaatt tattctataa aaaatgcgta aagtttttat tcttttttga aaaaacaaat
     3781 gagtgccaaa tatacacttt atttcttagt ttttgcagtc gtattttgcg tcttattctg
     3841 tgcatctgac aagataaata aggcaatgta ataaaatggc ataaatggaa ttttatatcg
     3901 cgcaagcgct ccaaaattga agctggtaaa accaacagca aaaccaaaga tgatagaaaa
     3961 aaccaaacaa aactgaactt caggattgct ggctattaat tttaaaaaac gtacaaatcc
     4021 agtcttaaat agcaaagtaa tcgttaaata tagcgtaaca attccttcta gtgaagcagc
     4081 caataaaatc ggctttcgga tttcccagat atatggtcta aacagagaaa cattaattgc
     4141 ttttgggaat acttttagca ttcccattgt tgaatattca atatcaccga gtgtataaaa
     4201 agagccattc tgttctttac ttgaatatac caaccagttt tgtgtatctt ttgctgttcg
     4261 cataatttct tcaaaggcat atcgttctgc ataacttgcc attgactgca agacaacaac
     4321 tccagcgcca agacctaaga caataaacag tggagttgag attgttttta gaaatggact
     4381 tttgatatta tatctataac gagcaaacac ccaaactgcc aatgcaggta aaaatgccaa
     4441 gattatatat actttaatag aaaaaactat gaaagcgcct aagagtgcta aaaagaggct
     4501 ttttagttta tttccttttt tgaagaaaaa atgatacata taatatgaca tcatgcctaa
     4561 accaccaata catattggct ccttcattac gcctgagccc caaaaaaaca cggaaggaat
     4621 aaacagacag gaaatagcca tttgtttttt gagatgagga tacatgtcat aaaacatgct
     4681 aaatattttc caacagccat atagagcaaa aagtgtaaac gtgatactgg tgagcatata
     4741 tgattgcaaa aagacaacta aacaagctga agttagccaa atgataatag cagttgtgcc
     4801 atcataaaaa tataagccac tttcaataaa atatttagac tcacttaaat aacgtaagga
     4861 ataaaaactt ttaggattca acatgatttg tggtatcatt tcaggatcgg taaacagcat
     4921 tttaattaca ccaagtgcat aaaagtaaaa tgtagtagta tcgccaccat tgtaataata
     4981 atcatacatt aaggcagtaa gtatgcagcc tgctaaacga acataccatg ctttcatcat
     5041 gtactccttg taatgttctg gctttttggc tatatatttt ttgagttgga catgtataaa
     5101 atacaaatac agtggcacca atataaaatc acttgcaaat attaaatctt gccttagcca
     5161 aaaattttct acttgctgtg ggaatagctg catttgttaa agtgtacttt atatcttctg
     5221 ggttggtgaa atgacacaca actacaacaa agatacagta aagcatctaa aatctaaaaa
     5281 gagatttata attagcaata cttttacttt ttctaataaa agtattcttt agcatctttt
     5341 ttctatcttt ggttggcatg ttattatctg ttgttgcgcc tatttatttt gcacaaaact
     5401 ctgtagaagt tcttgtaaag ctaattgttt cagagtgttc gttgttacag atggactttg
     5461 aaatcatact tatagaagat ggaagcactg ataacacttg ggaaaaaatt gaagtacttt
     5521 catctgacat acctcagctt agaggtatta aattaagtag aaattttgga cagcactacg
     5581 ccattgcgtg cggcattgaa caagcaaaag gcgattggat tatcgtaatg gatggcgact
     5641 tgcaagacca tccaagtgcc attaaacaac ttcttgcaaa agctaatgaa ggttatgata
     5701 ttgttgttgc aaaacgcatc agccggcaga cttccttttt gaacaaaatt tactcaatca
     5761 ttttttggaa aatattaagc taccttactg gaagacctct acaccatagc atttcgaatt
     5821 ttggcatcta tcatagacgt gttatcgatg ctattatttc gatgcgtgat gccattcgat
     5881 ttttcccaac tatggtcgat tgggttggct ttaagaaaac gactatagat atgccacaac
     5941 aagcacggca tgaaggaaaa tcaacctatt catttaacaa gatgatacgt ttggcgctag
     6001 atgttgtatt agtgaattct gaaaagccat tgcgattagt tactaaaact ggcatcttta
     6061 tatcaatcat ttcatttgtt gtaggcatta tttatttata caaacatctc acacatcaaa
     6121 tcgaagtgcc tggctatgca agtatcatta tttctatttg gtttttagga ggtttaatca
     6181 tttgtattct aggtattttg ggattgtata ttggcaaaac tttcgatgcc gtaaaaaaca
     6241 gaccttacta catcattgaa aaaacaactc ggtgatggaa ataatacaag cactcacctg
     6301 ggattctgat ttttttcaac tacgcattgg taaaatcgat gagctcagta aattgcaatc
     6361 tattacagca gcagcaatta atttgtttga tttaatttac attgaacaaa cagcacaaaa
     6421 tctagacacg tttttagaaa atcaaacaaa ttttaatatt acatatacgg acataaaaac
     6481 aatttataga aaacaactgc ttcataacgt tacttcagtt gacaaacaaa taacagtgta
     6541 tccatttgcc acaactaacg acgctttaat taatttatct atacaagcag gcgctttttc
     6601 acggtttaag ttagatccaa attttggtgc tactagctat aaaaatctat atcgacaatg
     6661 gattgaaaat gctgtacaaa aagtaagcgc tgatattgtt ttggtatatg gtgatataca
     6721 atatccaaaa ggatttatca cattggtttt taaagaaaat ttggcgaaag tcggtttaat
     6781 tgcagtggat cagcaacatc aaaatgaagg cattggcaaa gccttaatgg aagctgccga
     6841 atactttgct attaaaaata attttactac attagaaatt ataacacaaa cagaaaacaa
     6901 gaatgcttgt gcattttacg aacgaatagg ttgtcatcaa acaaacattt tatatacaca
     6961 ccatcattgg aagaagcatg caaataccat ttaacaaacc atatttaaca ggaaaagaaa
     7021 tcacttacat acaagaagct gccgctatag gtaagctttc aggtgatggt gtttttacaa
     7081 aaaagtgcga acgttttttt gaagaaaaat atggttttaa aaagtcgatg cttacgtctt
     7141 cctgcactga tgcgctagag atggcagcca ttttaatcaa tattcaagaa ggtgatgaag
     7201 tgattatgcc aagctttact tttgtttcta ctgcaaatgc ttttataatg cgtggagcaa
     7261 aaattgtatt tgcggatagt agtgccgaac atccaaatat tgatgcaaca aaaattgcaa
     7321 acttaattac acgaaataca aaagcaattg ttgtggtgca ctatgctggc gtagcttgtg
     7381 atatggaaag tattatggca ttagccaatc aatataattt attcgtcatt gaagacgctg
     7441 ctcaagcgat agacgcttat tacaaaggca aaccactagg aagcattggt catttagcag
     7501 cgttctcatt tcacgaaaca aaaaatatca ttgcaggtga aggaggcatg ctcatcatta
     7561 atgacgaaaa atttattgaa cgtgctgaaa taatacgaga gaaaggaact aatagaaata
     7621 aattttatcg tggtgaaatt gacaaatata attgggtgga tattggttct tcctttctac
     7681 catctgaaat tacagcagcc tttttatggg cgcaaataga agccatagac gatattcaac
     7741 aaaaacgaat ttccatctgg aatacctatt atgaagcact ccttgcttat gctgattttt
     7801 ttcaattgcc tgtcattcat gaatatgcca gcaacaatgc tcatgcattt tatttggttt
     7861 gccagcaagc ggaagaccga aaccagctta tagacttttt tagaagtaaa aatattttag
     7921 cagtttttca ttatcaatct cttcataaaa gtccatattt tatagacaaa cacgatgctc
     7981 gtattttgca agaagcggat agatatagcc aatgtttact gcgtttaccg ctattctatg
     8041 agttatctac agaagaaatt acacatacta ttattccaac cattacaaca tttgttgagc
     8101 aatatgccta ttaataaaaa aaatatctta atagcgtttt ttggcgtgat aggatttgct
     8161 tttatgatca ataccatcaa tgctgatttt gtgaaaaatg ctagcttctt agcagaaaat
     8221 gaacgcttag tacatggaca aaccatttac tctattgaca atagttttta cttgccacaa
     8281 ataaaaaatt ggctcgcagg acatggtttc acttgcgacg ctagcgacaa aaaatatttg
     8341 gtgagacgaa cccctgttta tcctatgttt tatggtgttc attatctttt atttggcgaa
     8401 aaaaatagtt tcttttttat aaaaatctca caaacgattt tgtttgcact gtctgccgtt
     8461 ctttttttat tggcagtaaa caatctgact agcaataaaa aaatggcatg gctcgcattt
     8521 ttattgtatg gatttaatcc aaccttgatt agcaatacct atttcacgct tacagagtcg
     8581 ataagtccag cattggtagt acttacatta tatttttttt caaaagcaat gcgcaattcc
     8641 aagctccgtg attggttgac aacaggcatt ttcttttcta ttgcatcgtt gtgtaggcca
     8701 cctattttct tctttggagg tgctgttgct attgccattt tattgctata ttataaaaat
     8761 aaaacaagct tacttaaaac aacttttgca tttggtgtag gcgtgttact tgtatttaca
     8821 ccgtatgtga ttcgcaatta caaagtaaca aatggcgaat ttattttact cgaaaaatat
     8881 tacggcgatc cgatgactta tggtttacaa aatattgaac tgagaaaatg gatttctaca
     8941 tggaccaatc ctgctgatta tagcgcagaa agtgtttcta ataaaatgtg gcaacaaatt
     9001 ttatccaagc gcgacaaaca aacattaatt gactctatgc ttattgcttt acctaaagca
     9061 gcatttacac attatagcaa aaatactgtc gcttccattt acaattgtgt ttatgatttt
     9121 tatgcctaca aatttacatc gccaacaaaa aaagaccttg ccattgctga gcaaaatgct
     9181 gtactgttta taaaaaaaat taatgacaaa catatcgctc agcatccatt ttcatattat
     9241 gtaatcatgc caatgaaaat gatgaaaagc attatttttc aaagcaatgc aggcacactg
     9301 tatttcttat atgattacaa aaacaattat cttttctttg ttataaaagc aatcttattg
     9361 ctattgaata ttttgttatt tatctcttta atcggcaata ttttatttat aaaaaaacac
     9421 ttaattatct atattgccat cttgtttttt gttagcatca attgttttta catgctgttt
     9481 gtcatcaatt attttgaagc gagatattta gtacctatgt tcccgttttt atatatttca
     9541 ggcgcaatat ttgccgttga aatgtatgaa tttatgaaaa agaaaattac acttctaaat
     9601 aacgcataag gctctcgtag cgttcttgca attgatgttc atattctgat tgatgaaata
     9661 cagcaacaac attatattga aatcgctcga aggaaagttg aatgtctttc aagtcatttt
     9721 tattgatttt taatgacact tgtatctgat ttgcttgact tgttgatacc aatatactat
     9781 gcaatatcat tgcattattt gattctacaa tttttgatat ttctgtaagc gagtaattct
     9841 ttgtatccat ttccaatact aaaatgccgc cactattatt tagagctgtg ttttgtgaaa
     9901 acgctgctaa tattttttgt ggtgtgctaa tgccgagata ttcatttgta gcattgacca
     9961 caggaaggaa gaaagaatca aattctgccg acgatttaat gacatcaaaa agatgcaagt
    10021 cgctggaaat tttatatgtt agaagatgtt catttaaagc atcaattaac aaggtatcat
    10081 cgagctctaa taaaaaattt tctgttatta aaccgcaata tatttccttg ttaagcactg
    10141 ctaatgctgg tgttttaaac tcttgcatta aatcaagtgc aaacccaacg gtatctgtca
    10201 gcctaacaaa tggaatggaa ttatctatgc attgcgtagc aatcaaggtg tagctttttt
    10261 gtaaaagtat aaaataatta tcgttctcgc ttaaaaatta aagttaaaac caaatgtcat
    10321 catccactta tatgcttttg gattatcgcg atgtgtaaca cgccacatca cgtcccaacg
    10381 aataaatttt aaaatatttt ctaaaccaaa accaacttcg atatatggaa tttttcctat
    10441 cggcttgatg aagctcgtat ttaattgatt aaatgccata ttttttgctg aagcatttcc
    10501 ccaaatacca cgcgtatgaa taacttcgcg caatttcaat tttctaatgc ctggaattct
    10561 attcagaaaa aagccgtcga aatgatgtga aaaattccat tgtaaatatc tatcactata
    10621 aaattcataa tcgttcatca cattaaatgc tgtgttgcta aaggcatatg tctgattgcc
    10681 atcatgcact tcatttaaca aaaatggcac tgtgccgaat acttttcccg cttgaatatg
    10741 catttcaaat cttccaatag tgcgtattgg aatgatttgt gcatacgaca atgtgagttt
    10801 atgataatta aattgactac ctaaaaattt attgatgcca tagacatatt caaattgtat
    10861 gacaggcact ttactagtat ttatgctact tctgaacaag gagtttttgt tgatatattt
    10921 ctcgccaatc gcaaatcgtg cattaataat cagctctgat tgccttagac tatgtaaggt
    10981 gtcagtcact aattctttat tggtgagcgc atatcgaaat ggcactaatc cttgcttgat
    11041 gtatctgttt tgaaatgcga tgccaaagga catgccttgt ttatactcta ttgaatagcc
    11101 gagtttggct tcttcaaaat atagcaaacg cacatctggt tttcttctgt ttattataat
    11161 actcaagata ttgtcagcag ccagtaaatc tgaagttttg ctgagttcac ttacatcgcg
    11221 tgtgtacgat gcgctaataa tttgacgcgg tcgtttgtca actaaaaatc gaacacctac
    11281 atcgcctttc acctttttat cgcgcaagcc gtatgcgata tgagcgctaa tttgagccca
    11341 cgaacacatc ttgtcgttgg tgcgcaaacc aattccaaaa cgccaacctt cgagcttatt
    11401 aaagcttact gtattcaata ttggtccgat ttctattggt cctgctttgt aatagccagt
    11461 caacacaaaa ttgataatat cgacatacgt tttataagca ggcaccgttt ttagagtatc
    11521 tatgatatga taaactgctg cttcgttttt tgataattgt ataaatcgag aagtgtccca
    11581 aaatgcttga tcttttttta tcgtatgctc atccattgtg agttcctctt taagcacagc
    11641 tatcatggaa tcattatatg ctgtgttgat tttaaaattt ctatatactg tattttttct
    11701 agtgacaatc gctggtgttt tttccaaagg tttaaattcg atgacaagat ggtctttggt
    11761 catcatccaa cataggccat ctacattatc aaaatcttgg ttgatttcta tttttttgac
    11821 gaaattaata ttaacatgag cagccattct tagctgaatg cttttaatag cataattatt
    11881 ttctgacaca ataaaactgc ctaaaaatgt gttatcacct tttgttttag gctcaaatag
    11941 catcttgtaa tgcacaatat tatcgagcac caaagtatcg attactttat atttataaaa
    12001 cgttttacat acgttagcaa ttgggctgat gaattgtttt tcgagaatag tataggcgtt
    12061 gtcatagaca tttatatctt gataagttgc atttagaaac tcattaaaac ttgcatcgct
    12121 aatgcctgac atttttgagg ctttaattat ttctcgtttt ttattgagtt tattggtata
    12181 ataataatcg gaaatatttt cggacaacat caatggcaaa aatggttctt cttctgaggt
    12241 actgtcaata ttgtcgaata caaacttgaa tggttttagt atttttcgat tcataaattt
    12301 gtcagacata tttttcactt ctaactctat tttattatac agttcgtagc tgtaattttg
    12361 taaatgtttt ttgtcattct tttctttatt ttctaatatt ttacgaataa gataatcttc
    12421 caagctttct ttgttggcaa caattacggt ttctgccaat gtgtttgcgc tcgatgacaa
    12481 caaaaaaaca agtctttgtt tgctttgcat actgaggcga attgtttttt cttcataacc
    12541 taaatatgcc gccgtgatag aatcgaaagc ctgtgtgagt ggcaatttaa aatagcctgt
    12601 atcatctgta gtgacgccaa tggtggtgcc tttaatgtaa atattagcat aacttatcgg
    12661 cgatttgctt acactatcca acacaatgcc atctataaat gtctgtgcat gtacagaaaa
    12721 atatcctaag aaaaaaagga ttagtatatt tgcttttacc gctttcaata aatggtgaag
    12781 ttaaaaaatt atcgctgaaa aaaatatttg taaatgaagt cattagaaat tatttcgtat
    12841 aacgtaaatg gcatacgctc tgctgtctcc aaaggtttaa tagattggat gaaaacgacc
    12901 aatcatgatg tgtatttatt tcaagaaata aaagcgaatg aattagatat accatcagaa
    12961 cactttcatg aaataggctt tcaaactcat tggttttctg cgcaaaaaaa aggatatagc
    13021 ggcgttggaa ttgccataaa aaatgaggtt ttacatgata atgtggtgaa gggaatggat
    13081 attcatcaat atgattttga aggcaggctg attcgtttgg atattggaga catcacgatt
    13141 attaacagct attttccttc aggttcgagc ggcgatgaac gacaacaagt aaaaatggca
    13201 tatctcgatg atatgtacaa gtacatacat gatattaagc aagatagtca aaaaataatt
    13261 ctagcaggtg attataatat ttgtcatacc gaaattgata ttcacgatcc aaaaggaaac
    13321 aaaaatagtt caggattttt gccggaagaa cgcgaatgga tgagcaaatt atttaatagt
    13381 ggttttattg atacttttag acatgttcat cctagtttat tgcatcatta ttcttggtgg
    13441 agcaataggg caaatgcacg tggcaataat aaaggttggc gtatcgatta tttagcaacg
    13501 acaaaagaaa tggaacaaca aattgaagat gcaaaaatat ttatggacgt caaacactct
    13561 gaccattgtc cgatttattt gaagatttcg atatagaaat ttatctctct ctgcctttgt
    13621 tggcttgcag ccaacaaact gccaagcgcg aataaaaagg caataggcga atattattct
    13681 aatgatttgt tggtcaagaa gactaacaaa tcaaggaata atttataatt acctctatgc
    13741 tagtttgttg gtcaaaaaga ccaacaaagg ctaggcttaa tttttaagct aactctcgca
    13801 agtatgcaga cttggtctat gcagatgcgt acttgtgcgg aattaaagta tgtatgtatc
    13861 gtactaaaaa acaatagaaa tgtttttctt gtaatgtggt tcgtgaggac accataagtg
    13921 ttcggaagat tcacatactc tgaataaata gataagctgt at
//
LOCUS       DAOQQV010000066        13927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1457_length_13927_cov_7.592488, whole genome shotgun sequence.
ACCESSION   DAOQQV010000066 DAOQQV010000000
VERSION     DAOQQV010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13927
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_13927_cov_7.592488"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1987
                     /locus_tag="PLL59_07875"
     CDS             230..1987
                     /locus_tag="PLL59_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23765.1"
                     /translation="MLLFVYLSLDSNAQANAFFNKGGLVYLKGGSSDVNPTLYVNGRL
                     TNQDGRFVNDDSKLELAGDFHNNPSSYNYESTGTELFSGAGDQTIRGTFNGTTGNDNQ
                     FYNLKINKTSATGEYIFISEFGTNANINAAGSLEFVSTNGIIRTQSTGSTSPYTGNYA
                     RALYIQNPSASAITGHSTSAGATTKYIEGKLIREVNASDTYFFPIGVEKTGLDGMEAI
                     SINMNALSMPAGSTTGLQAYIRPANTLSYSSDLIANGDVLFYDIGGYDVLTNNNHFSQ
                     CVSGPEGYDDVALIDGAITHEWMVTPTVAPTSVSYDLTVVPGSTLENQVNYAVMGTAC
                     NSLYPKAQYLARDGRIGGNSSFGPTYIFDVPNLLGLVQAPTLKTITGQNGFSRFRLFG
                     ASDNNTSLPVELMSFTISPINNEYFILDWKTASELNNSGFFVQRSTNGITFETIGWID
                     GNGNSNSMHAYSFHDRNVEQNITYYYRLKQVDFNQNASFSATLSGILKGENFEVNSIT
                     PNPTNVNAEASIFMPENGSVEINIFNILGQNVQTSHVDLSKGLNKVTIESASLAPATY
                     LIKFQMNDKTITKKLLKNL"
     gene            1998..3332
                     /locus_tag="PLL59_07880"
     CDS             1998..3332
                     /locus_tag="PLL59_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23766.1"
                     /translation="MKKTLYTFLLTLTSIATFAQSGNVGIGTNNPNVSAILDISDAAR
                     GLLIPRITLASTTDAATITTPAQYLVVFNTATAGAAPNNVTPGLYYNAGTSGAPNWVR
                     LQTKADNDWTKATTTATPAIKTDNQYITGYAGIGDFSTTNPLKNLHIKHTASTDGLLL
                     QNTTGNIGSSTNIFFSTYADIVAGTNRPGAKIAAIDDNYSAHLTFSTKIPGADANAMT
                     ERVRVRSNGEVQVTNLAGTGTSMVAATSDGTLIRQNKFLDVTVHDVYVSSYESCSNCD
                     KFIWFSRPGGSSGDDRYDNPIYEKNMWIAPYNGRIVSIRLGVDPTSSNSGVEINKGRI
                     VFEHACNLPDADLSYYTNMSKSGSSTYANVSNWNGGEYEVLCGTPNFTITSPNRGRFN
                     LNEGQVINWNSKGSNFFSFTKGQYLAIGVSGEYIEDNSYHMTVVWEYEFDEN"
     gene            complement(3530..4309)
                     /locus_tag="PLL59_07885"
     CDS             complement(3530..4309)
                     /locus_tag="PLL59_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HMY23767.1"
                     /translation="MSALGLIETMKVVNGRPVFKNLHISRLAAGLKSINVPINEYQLE
                     DRILRLLKYECVDNGLKNFRLRIEVHKNRAHEYMPDIAALKWNCTLRPLESVKYTWNT
                     DKFILTILPKHKKMIDAFKNLKHTDRAIYNEALQYARENDCNDALVLNENGTIADASI
                     YNVFMVKDGIVYTPPLADAPVAGVLRELLLTRLDTITIQEKSITIQDLYAADELFLTN
                     ALRGIRAVDEFDGTVYESTITKQVFTAFQQVVSEHFGEHLV"
     gene            complement(4379..4960)
                     /locus_tag="PLL59_07890"
     CDS             complement(4379..4960)
                     /locus_tag="PLL59_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HMY23768.1"
                     /translation="MNKHYFILLLSFIALYSCTDETKNPVKPPLTAVIEQDINASHAN
                     YQYYSFATGDTLPFADSSTANWDIAFKGTSVIVNGGTGKAGVGGVIIMDTLFSEVTEA
                     PTAGYVQESASAAIPTGSNNGWYNYAGPPNHTITPLAGKVFVIKTGAGKYAKMEFISY
                     YKGAPATPNGLLDISKYYTIRFVYQADGSTSLK"
     gene            complement(4972..7077)
                     /locus_tag="PLL59_07895"
     CDS             complement(4972..7077)
                     /locus_tag="PLL59_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY23769.1"
                     /translation="MSKLLTLLLFCAVATVSNAQELDTVINENVNNNIEEIVVTATKT
                     ETKIQNTTVPMSVIASKYIKASGTIKLGDLLQEQSGLQLSNFLGTGVQLQGLSAEYTL
                     ILINGEPIIGKNAGVVDLSRIVVNNIKRVEIVKGPVSCLYGSDAIGGVINIITEDVGK
                     LKNTVDLSVQYKSPAILTTSLFSSFSKKKVSFENSVVHNYSNGFHTNKETIFKEGAPH
                     NDFTLSNKLTVRANERIQAGIGLKYYYSKLRNQDNYLNKSDSMIVAKQEDKINEFNFS
                     PFFKFVKNSIVLNIKNFNSYYTSTSNFSGFDLNDELYDDSFKQLLSKLEVQFDYTKKG
                     HLFTAGTGGFYNLVLSNRNTKKIHQHQEFLFFQYQYDWKSKIIFNVGNRIDFPSDYAI
                     QWLSPKASARFNVNKYFAIKISAGRGYKAPDVRQQYLNFTNALVGYSIFGAHIAADKL
                     SELEELGQIHSYSIPISEIKNLSPEKSWSANAEIEIKPIPDKLTISANYFRNQIDNMI
                     EAIPTVIKTNNALIYTYYNLNKVYTQGVDAVVNYQIIKGLSLNVGYSFIDAKDEGILK
                     KLKDGKIFRRDPETLETVRVTKKEYGGLFDRSKHSFTCKVYYENTKHAFSTYLRIIYR
                     GKYGWNDNNGNQILDVASEYAKGYTLMNFSFTKSFKNKYHVMVGIDNMLNTKRPIHVP
                     SMNGITAVVGLTINFLNTK"
     gene            complement(7085..7969)
                     /locus_tag="PLL59_07900"
     CDS             complement(7085..7969)
                     /locus_tag="PLL59_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23770.1"
                     /translation="MASPTDVSKFEKDKQSIKNLCGCFEVDFKYKETFATAKDYKLKD
                     KYVNEALEWVSYEPQENGSIMMQHILIIDESSIIKHWRENWSFENNEIYKYNKDYHWS
                     AITLPAENVKGTWTQKVYEVDDRPQYDGVATFNYFDNTTLWQSKIDAPLPRREYSTRA
                     DYNVLNRYNRLYITDSGYLHEQDNQKIARHDNTKDTLIAEEKGYNVYRKVEDSKCKAG
                     IDWWEKNKIYWLDVRTVWNALLKDRNEVKVNRVVEGKTLHDKLFALNLELLKEKKYNS
                     EKAKIAIQKAINEHLVSK"
     gene            complement(8112..9008)
                     /locus_tag="PLL59_07905"
     CDS             complement(8112..9008)
                     /locus_tag="PLL59_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMY23771.1"
                     /translation="MLRFIRLLIETNLFIAIAGACFMWANVVFLDLHVASLYILSAQI
                     FFSTWFVYQISRWIYFKKGEYSNKDELVAQWFEKYPNFNKFTIYGAGFATVIFTLFLR
                     LETIFVLGVIGAISVLYPVQILKPLGITTRLRDFPFVKIFLIAFVWSATSVILPYTES
                     KEYVSIDKVQVLLMFVVQFIYILFITLPFDINDAESDKSSDMKTIPSVFGITISKWIA
                     FFLGLLYLIAIVFMYMLMNWNHLPNKYLSEISIISIITMIVALQIFTFLKSDKVSKFW
                     IKMVYDGSMILYFLLIYLLNIR"
     gene            complement(9078..10160)
                     /locus_tag="PLL59_07910"
     CDS             complement(9078..10160)
                     /locus_tag="PLL59_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HMY23772.1"
                     /translation="MKYAIDKKAEEFLFKYLNNASPTGFEAEGQKIWLDYLKPYIDEY
                     HVDHYGTVYGVINPEAEFKVVIEAHADEISWFVSYIGDDGMINVIRNGGSDHMIAPSM
                     RVNLHTKKGIVKGIFGWPAIHLRDLAKDTSPSIKNITIDVGAKDKKEVESMGIHVGTV
                     VTYEDELMILNKKFYTGRALDNRLGGFMIAQVARLVKESKSKLPFGLYICNSVQEEVG
                     LRGAEMIVNKIKPNIAIITDVCHDTTTPMIDKKVHGDFKCGKGPVITYAPAVHNIIRE
                     LIIDTAEKEKIPFQRDAASRVTGTDTDAFAYNGGGVPSALISLPLKYMHTTVETVAKT
                     DVEDVIKLIFESLKKINPKMRLKYLS"
     gene            complement(10455..11504)
                     /locus_tag="PLL59_07915"
     CDS             complement(10455..11504)
                     /locus_tag="PLL59_07915"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMY23773.1"
                     /translation="MKKLFYLLSLPVIFITACIPTGNIPTYPAYDPSTIINKIVISSC
                     CYSGGNLSIFRNMQAKDADLYIAMGDNMYADNIISNPDYPNWIQSQYNLLSNNMDYSN
                     FRKSIQSIATWDDHDYGLNNAGKEFPYKLQSKEKFMSFWQIQSTHDIRTHDGVYTSYL
                     YGDAAHRVQVILLDCRNFLDVLSGEPIAPTTDTSKTILGATQWAWFRQQLQTPAKIRI
                     IVSSTQFATGHNGWETWANFPHEMEKFYQALRDANAEGAFVVSGDVHYAEYSKRTPAN
                     LYPIYDFTSSGLTHTEYNPSNNPYRIGDAYRQYNFGVVNIDWNASPVNITLETCAFNG
                     TVAKQISIPLDELKF"
     gene            complement(11553..13103)
                     /locus_tag="PLL59_07920"
     CDS             complement(11553..13103)
                     /locus_tag="PLL59_07920"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HMY23774.1"
                     /translation="MTHEQKLEALEVKKKEALLGGGQKRIDDQHKKGKLTARERIELL
                     LDAGTFEELGMLVTHRATDFGIDKEKYYGDGVVTGYGKVNGRLVYVFSQDFTVFGGSL
                     SETHAEKICKIMDLAAKTGAPVVGLNDSGGARIQEGVVSLGGYADIFHRNVQFSGVVP
                     QISAIMGPCAGGAVYSPAMTDFTLMVENTSYMFVTGPNVAKTVTHEEVTSEELGGAQT
                     HATKSGVTHFTAKNEVECIQQVKQLLSYLPQNCEDTAPVYPYQTEGSEKRDALSSILP
                     SNPNQPYDIREVIQHTTDENSFFEVHKNFAENLVVGFARIAGRSIGIVANQPAYLAGV
                     LDINSSRKGARFTRFCDAFNIPLLVLVDVPGFLPGTDQEWHGIITNGAKLLYALSEAT
                     VPKVTVITRKAYGGAYDVMNSKHIGADMNFAWPTAEIAVMGPKGAAEIIFKNEIKQAE
                     NPTQKLQEKVDEYTDTFANPYKAAARGFIDEVIKPEETRIKLIKAFQMLENKVVNSPK
                     KKHGNIPL"
     gene            complement(13166..>13927)
                     /locus_tag="PLL59_07925"
     CDS             complement(13166..>13927)
                     /locus_tag="PLL59_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HMY23775.1"
                     /translation="TMKNLLLSIVFLVFVFINKVNAQIVNIESQRYATDTTGWAGDYN
                     LGFTFGKQKEKYFSFFNSAHVQYKSKRSLYLLLGSLDILKKGNEKFVNAGFFHFRYNY
                     KIKHWLRWEAFTQVQYNKLIGLRMRYLLGTGPRFKLVQIEKFKTYLGTLYMLEYEINN
                     DKSQKLLQGRFSAYLSFSIRPIKTIEFISTTYYQPRFDEIKDYRIATENAILFKFHRI
                     LSFGMNFKLNYDAAPPVGAPTKLTYQLDNKFKIDF"
BASE COUNT         4506 a         2468 c         2241 g         4712 t
ORIGIN      
        1 ctgacttgat atttatcaaa taatctacaa tcacattaag aaaaaacaaa aaacaaaaaa
       61 tacaacaatc tgattatcaa cactcaacac atattgtgtg tttatttgac ttttgtcaaa
      121 cctaaaaaat tgacaggtct ctcaattatg attaatttta cgtaataatc ttattagcct
      181 tagattaaac ttaatatatg acaaaacttt acacttcaaa catcctcgtt tgcttctttt
      241 tgtttatctc tctttagata gcaatgctca agctaatgcc ttttttaata aaggtggttt
      301 agtgtactta aaaggtggta gtagcgatgt aaatcctacg ctttatgtta atggtcgatt
      361 gaccaatcaa gatggtcgtt tcgttaatga tgactctaaa ttagaacttg ctggagattt
      421 ccataataat ccatcatctt ataactacga atcaacaggt acagaattat tttctggcgc
      481 aggcgaccaa actataagag gcacttttaa tggcacaact ggaaatgata atcagtttta
      541 caatttaaaa ataaataaaa cttccgctac tggtgaatat atttttatta gtgagtttgg
      601 cactaatgca aatattaatg ctgcaggcag cctcgaattt gtaagcacaa acggcattat
      661 cagaacgcaa tctactggct ctacatcgcc ctatactgga aattatgcaa gagcattata
      721 tattcaaaac ccatctgcca gcgccattac tggtcacagc actagcgcag gtgcaactac
      781 taaatatata gaaggaaaat taatcagaga agtgaatgca agtgacactt atttcttccc
      841 aattggcgta gaaaaaacag gattagacgg catggaagca atctctatta acatgaatgc
      901 actaagcatg ccagctggtt ccactactgg cttgcaagct tatattcgtc ctgctaatac
      961 attgtcttac agcagtgatt taattgctaa tggcgatgtg ctattttatg atattggtgg
     1021 ttatgatgtt ttaaccaata acaaccactt ttcacaatgt gtaagcggtc cagaagggta
     1081 tgatgatgtt gctttaattg atggtgctat tacacacgaa tggatggtca cgccaactgt
     1141 tgcaccaacc tcagtttcgt atgacttgac tgttgttcct ggttcaactt tagaaaatca
     1201 agtaaattat gctgtaatgg gcacggcttg taattcctta tatccaaaag cacaatactt
     1261 agcacgtgat ggcagaattg gtggcaatag ttctttcggt cctacttaca ttttcgatgt
     1321 gccaaactta ttaggtttag tacaagcacc tacattaaaa actattactg gtcaaaatgg
     1381 attttcaaga tttagattgt ttggcgcttc tgacaacaat acttctttac ctgttgaatt
     1441 gatgagtttt accatttcgc caattaacaa tgagtatttt attttagatt ggaaaactgc
     1501 atcagaatta aacaattctg gattttttgt tcaacgcagc acgaatggta ttacttttga
     1561 aactataggt tggatagatg gcaatggcaa tagtaatagc atgcatgctt attctttcca
     1621 cgatagaaat gtagaacaaa atattactta ttattataga ttaaaacaag tagattttaa
     1681 tcaaaatgca agcttttctg caacgctatc tggcatcttg aaaggtgaaa attttgaagt
     1741 gaatagtatt acaccaaatc cgacgaatgt aaatgcagaa gctagtattt ttatgcctga
     1801 aaatggaagt gttgaaatta atatattcaa tatattaggt caaaatgtac aaacttccca
     1861 tgtagattta tctaaagggc ttaacaaagt tactattgaa tctgcatcgt tggcgcctgc
     1921 tacttatttg attaaattcc aaatgaacga taaaacaatt acaaaaaaat tattaaaaaa
     1981 cttataaata taaccgtatg aaaaagacac tctacacctt cttacttaca ttaacaagca
     2041 tcgctacttt tgcgcaaagt ggtaatgtag gtataggtac caataatcca aatgttagcg
     2101 caattttaga tattagtgat gctgcacgtg gtttattaat tcctcgtatt acattagcta
     2161 gtacaactga tgctgctacc atcacaacac ctgcacaata tcttgttgta tttaataccg
     2221 caactgctgg tgctgcacct aacaatgtta caccagggct ttattataat gctggcactt
     2281 ctggtgcgcc taattgggtg cgcttacaaa caaaagctga taatgactgg acaaaagcaa
     2341 ctaccacagc aacgcctgca attaaaacgg ataaccaata tattacagga tatgctggga
     2401 ttggcgattt ttctactaca aacccattaa aaaatttgca tataaaacat actgccagta
     2461 cagatggttt attgttacaa aatacaactg gcaatattgg tagtagcaca aatatatttt
     2521 tctctactta tgcagatata gttgcgggta ccaatagacc aggagccaaa attgcagcaa
     2581 ttgatgataa ttatagtgca caccttactt ttagcactaa aatacctgga gcagatgcaa
     2641 atgcaatgac cgaaagagta cgtgttcgta gcaacggaga agtgcaagta actaacttag
     2701 caggtactgg tactagtatg gttgcagcaa cttctgatgg tactttaata cgacaaaata
     2761 aatttttaga tgttacggta catgatgttt atgtaagttc atatgagagt tgtagtaatt
     2821 gtgataaatt tatctggttt tcaagaccag gtggctcaag tggagacgat agatatgaca
     2881 atcctattta tgagaaaaat atgtggattg caccatataa tggacgaatt gtatcgatac
     2941 gcttaggagt agatcctact tcaagtaata gtggtgtaga aattaataag ggtaggattg
     3001 ttttcgaaca tgcatgcaat cttccagacg ctgatctttc ctattatact aatatgtcta
     3061 aaagtgggag tagtacatat gcgaatgtat ctaattggaa tggaggagaa tatgaggtat
     3121 tgtgtgggac tccaaatttt actataacgt cgcctaacag aggcagattt aatttaaatg
     3181 aaggacaagt tatcaattgg aactcaaaag ggtctaattt ttttagcttc actaaaggac
     3241 agtatttggc gataggtgtt tctggagaat atattgaaga taattcatat catatgacag
     3301 ttgtttggga atatgaattt gatgaaaatt aatggtataa tatctaatgg tctgtgtcct
     3361 caccgaccat ttttattata atagctcaat gtcattccct tgcaaaaggg aatctcattt
     3421 tatcatataa catttccttt gggattccca ctttcgtggg aatgactttc actattgaac
     3481 ggtgtcatac caaccgtata ttttaacgca agtaagaatt tacttaaagc tacaccaaat
     3541 gttcgccaaa gtgctcgctg acgacttgct gaaatgctgt aaaaacctgt ttggtgatag
     3601 ttgattcgta aactgtacca tcaaattcat caacagcacg aatgccacgt aatgcatttg
     3661 tcaaaaagag ttcatcagcg gcatataagt cttggatcgt gatagatttt tcttgaatag
     3721 taatcgtatc taaacgcgtg agtaataatt cgcgcaaaac acctgcaact ggtgcatctg
     3781 ctaatggcgg cgtatagaca ataccatctt ttaccataaa tacattataa atagaagcat
     3841 cagctattgt tccattttca tttaatacca atgcatcgtt acaatcgttc tcacgagcat
     3901 attgtaaagc ctcattataa atggctctat ctgtatgttt aagattttta aaagcatcta
     3961 tcattttttt atgctttggc aatatggtga gtataaattt atccgtattc cacgtatatt
     4021 ttactgactc taaaggccgt aaggtgcaat tccacttcaa tgctgcaata tcaggcatgt
     4081 attcatgtgc tctatttttg tgcacttcaa ttcgcagcct aaaatttttc aaaccattat
     4141 cgacacattc atattttaat agtctcaaaa ttctatcttc taactgatat tcattaatag
     4201 gaacattgat tgattttaag ccagcagcca aacgacttat atgcaggttt ttaaaaactg
     4261 gcctgccatt tacgactttc atcgtttcta ttaatcctaa tgctgacatc ttgattatgc
     4321 taatataaaa aaagagacgt atgcaacgtc tctcttatat tgtgaaataa aagttagctt
     4381 atttcaatga agtgctaccg tctgcttgat agacaaaacg aatagtataa tatttagaga
     4441 tatccaacaa gccattcggc gtagcaggtg cacctttgta atagctgata aattccattt
     4501 tcgcatattt accagcacct gtttttataa caaatacttt tcctgctaat ggtgttatag
     4561 tatggttagg cggaccagca taattatacc aaccattgtt gctacctgtt ggtatagctg
     4621 cgcttgcact ttcttgcaca tagcctgctg ttggtgcttc tgttacttca gagaataaag
     4681 tatccattat tattacacca ccaacgccag cttttcctgt gccaccattt acaattacag
     4741 aagtgccttt aaatgcgatg tcccaattcg cagtagatga atcagcaaat ggaagtgtat
     4801 cgcctgtggc aaaactataa tattgatagt ttgcatgcga agcgttaata tcttgttcga
     4861 taacagctgt taatggtggt ttaacaggat ttttcgtttc atctgtgcaa ctataaagtg
     4921 caataaaact cagcaataaa ataaaataat gtttgttcat gttgtgttaa tttatttagt
     4981 gtttaaaaaa ttaatagtta atcctacaac agccgtaatg ccattcatag agggcacatg
     5041 tattggacgt tttgtattta gcatattatc gatgcctacc attacatgat atttattttt
     5101 aaatgattta gtaaatgaaa aattcattaa ggtatagcct ttagcatatt ctgaagcgac
     5161 atctaaaatt tggtttccat tattatcatt ccaaccatat ttgcctctgt aaataatacg
     5221 tagataagta gagaatgcat gttttgtatt ttcataatac actttgcagg tgaaagaatg
     5281 cttacttcta tcaaacaaac cgccatattc ttttttagta acacgtacgg tttctaaggt
     5341 ttccggatca cgtctgaata ttttgccatc ttttaatttt ttcaaaatgc cttcatcttt
     5401 cgcatcaata aaactataac ctacattgag tgaaagtcct tttattattt gatagtttac
     5461 gacagcatca acaccttgag tatatacttt attcaagttg tagtatgtgt aaataagcgc
     5521 attattcgtt ttgatgacgg taggaattgc ctctatcata ttgtcaattt gattgcggaa
     5581 ataattagct gagatggtca acttatcagg aataggcttt atctcaattt ctgcatttgc
     5641 agaccatgat ttttctggac ttaaattttt aatttctgaa attggaattg aataggaatg
     5701 gatttgacct agctcctcta attcgcttaa tttatctgcg gcaatatgtg caccaaaaat
     5761 agaatagcca actaaggcat tagtgaaatt taaatattgc tgacgtacgt ctggtgcttt
     5821 atagcctctg ccagctgata ttttaatggc aaaatattta ttaacgttaa agcgcgcaga
     5881 agcctttggc gacaaccatt gtatagcata atcacttgga aagtcgatac ggtttccgac
     5941 attaaaaatg attttagact tccaatcata ttgatattgg aaaaacaaaa actcttgatg
     6001 ttgatgtatt tttttagtat ttctattcga caaaacaaga ttgtaaaatc cacctgttcc
     6061 ggctgtaaat aaatgacctt tttttgtata atcaaattgt acttctaact tgctaagcaa
     6121 ttgtttaaaa ctatcatcat acaattcatc attcaaatca aaccctgaaa aattacttgt
     6181 actcgtatag taggaattga agttttttat gttgagtaca atactatttt tgacaaactt
     6241 aaagaaaggt gaaaagttga attcattgat tttatcttct tgcttggcaa caatcataga
     6301 atcagattta ttaaggtaat tatcttgatt gcgcagttta gaataatagt attttaggcc
     6361 aattccggct tgtatgcgtt cgttggcacg aactgtcaat ttattactta aggtgaaatc
     6421 attatgcggt gcaccttctt taaatatcgt ttctttattc gtgtgaaagc catttgaata
     6481 attgtgtacg actgaatttt caaagctcac tttcttttta gaaaaagatg aaaataatga
     6541 agtcgtaagt atggcaggcg atttatattg aacagataaa tcaacagtat ttttaagttt
     6601 tcctacatct tctgtaatga tattgataac gccaccaatt gcatcacttc catataaaca
     6661 agaaacagga ccttttacta tttcaacacg tttaatatta ttgaccacaa ttcgcgataa
     6721 atcaacaaca cctgcatttt tgccgattat tggttcgccg ttaataagta ttaatgtgta
     6781 ttctgctgat aaaccttgca attgcacacc agttcctaaa aaattcgaca attgcaatcc
     6841 tgattgttct tgcaataaat cgccaagttt tatggtacct gatgctttga tatacttact
     6901 cgcaatgaca ctcatcggaa cagtagtatt ctgtatttta gtttctgttt tagtggcagt
     6961 gactacaatt tcttcaatgt tattattgac attttcatta attacagtat ccaattcctg
     7021 cgcgtttgaa acggtagcca ctgcacaaaa aagaagtaag gttaatagct tagacattta
     7081 ttatttattt tgaaaccaag tgttcgttta ttgctttttg aatggcaatt tttgctttct
     7141 ctgaattgta ttttttctct ttcaacaatt ctaagttcaa tgcaaaaagc ttatcatgta
     7201 atgtttttcc ttcaactact ctattcactt ttacttcatt tctatctttt aatagagcgt
     7261 tccaaaccgt gcgaacatca agccaataga ttttgttttt ttcccaccaa tctataccag
     7321 ctttgcattt gctgtcctca actttacgat acacgttata acctttttct tcagcaataa
     7381 gcgtatcttt tgtattatca tgacgagcaa ttttttgatt atcttgttca tgtaaatagc
     7441 ctgaatcagt gatatacaaa cgattgtatc gatttaatac attataatca gcgcgtgtag
     7501 aatattctct gcgtggtaat ggcgcatcaa ttttagattg ccataaagta gtattatcaa
     7561 aatagttgaa tgttgcaacg ccatcgtatt gtggtctgtc gtcaacctca tacacttttt
     7621 gcgtccaagt accttttaca ttttcagcgg gtaatgtgat tgcgctccaa tgataatctt
     7681 tattatattt ataaatttca ttattctcaa aactccaatt ttctctccag tgcttgataa
     7741 tagaagattc atcgataatt aaaatatgct gcatcatgat ggaaccattt tcttgcggtt
     7801 catacgaaac ccattctaag gcctcgttga catatttgtc ttttaacttg taatcttttg
     7861 ctgtagcgaa tgtttcttta tatttaaaat caacttcaaa acagccacaa agatttttga
     7921 tactttgttt gtctttttca aattttgata catctgttgg cgatgccata gctacggatt
     7981 gtagcaagaa aagcgcgatt aggacattaa attgtttcat tttatttagc tttttaaaat
     8041 taagtgcaat attacagcat tcttaattgt atttagtcta aataaacaaa ataccaaaca
     8101 aaatacctaa tctacctaat attaagtaga taaattaaaa ggaaataaag aatcatgcta
     8161 ccatcataca ccattttaat ccaaaattta gaaactttat ctgatttaag gaaggtaaag
     8221 atttgtaaag ctacaatcat agtaatgata gaaatgatgg agatttcaga taaatattta
     8281 ttgggaagat gattccaatt catgagcata tacataaaaa cgatagcaat cagatagagt
     8341 aagccaagaa agaaagctat ccatttagat attgtaatac caaaaaccga tggaatagtt
     8401 ttcatgtcag atgacttatc agactctgca tcattgatat caaaaggcaa tgtaataaag
     8461 agaatataga taaattgcac tacaaacatt aataaaactt gtactttatc aatcgacaca
     8521 tattcttttg attctgtgta tggtaagatg acagaagtgg cactccaaac aaaagcaatc
     8581 aaaaatattt taacaaatgg aaaatcacgt aaacgcgtag ttatgcctaa aggcttaaga
     8641 atttgtactg gatataaaac tgaaattgct ccaataacac ctaatacaaa tattgtttcc
     8701 aatcgaagaa agagtgtaaa aattacagtt gcgaaaccag caccataaat cgtaaactta
     8761 ttaaagtttg gatatttctc aaaccactgc gccactaatt cgtctttgtt agaatactct
     8821 ccttttttaa aataaatcca acggctgatt tgatatacaa accaagtaga aaagaaaatt
     8881 tgagcagaca aaatatataa tgatgctacg tgcaaatcaa gaaaaactac atttgcccac
     8941 ataaagcaag cgccagcaat ggcaataaat aagttagttt ctattaataa tctgatgaag
     9001 cgtagcataa aaaaatccgt gtaggcaaag actacacgga tttgaatata caatttattt
     9061 tattgcaatc aattttttta acttaaatac ttcaatctca tttttggatt tatctttttt
     9121 aatgattcaa aaattagttt gataacatct tcaacgtcag tttttgcaac tgtttctact
     9181 gtagtgtgca tatatttcaa aggcaaggaa atcaatgcgc taggaacgcc accaccatta
     9241 taggcaaatg catcggtatc ggttccggta actctacttg cagcatctct ttggaaagga
     9301 attttttctt tctcagctgt atcgataatt aactcacgga tgatgttatg tacagctggc
     9361 gcataagtaa taacaggtcc tttaccgcat ttaaaatctc catgtacttt tttgtctatc
     9421 attggtgtag ttgtatcatg acatacatca gtaatgatgg caatatttgg ttttattttg
     9481 ttgacaatca tttctgcacc acgcagacct acttcttctt gtacagaatt acagatgtat
     9541 aagccaaatg gcaatttcga tttcgactct ttaactaagc gtgccacctg agcaatcata
     9601 aaaccaccta atcggttatc caaagctcgt cctgtataga attttttatt taaaatcatc
     9661 aattcatctt catacgtaac aacggtacct acatgtatgc ccatgctttc tacttctttt
     9721 ttgtctttag caccaacgtc gatagtaatg ttttttattg aaggtgaagt atcttttgct
     9781 aaatcacgga ggtgaattgc tggccagcca aaaataccct taacaatacc ttttttagta
     9841 tgcaaattaa cgcgcattga tggtgcaatc atgtggtctg agccaccgtt acgaataaca
     9901 ttaatcattc catcatcgcc aatataacta acaaaccatg atatttcatc agcatgtgct
     9961 tctataacca ctttaaattc tgcttcagga ttaataacgc catacactgt tccataatgg
    10021 tcaacgtgat attcgtcaat atatggtttg agatagtcta accaaatctt ttgaccttca
    10081 gcttcaaatc ctgttggtga agcattgttc agatatttaa ataaaaattc ttctgctttt
    10141 ttatcaatag catatttcat acgatgtaat taaaaaattt aaaaatatat acaaaccata
    10201 cttaaaaact atatatgaaa ttaaacatgg ttcacaaagt tacaattaat ctaaataaaa
    10261 tgaaatatca ttgtaattat taaatactgt gtgccaatcc gtattattaa atactattaa
    10321 aatcgtataa aatgaataag gtataatcca aaagtggata aaaataaaat ttgttgaatt
    10381 catgtgataa acactattaa taaaaaaaag caggaacgaa ttcctgctga tttgttcaat
    10441 agattttgaa aaatttagaa ttttaattcg tctaaaggaa tgcttatttg cttcgcaacg
    10501 gtgccattaa atgcacatgt ttctaatgta atattcactg gagacgcatt ccaatcgata
    10561 ttaactacac caaaattata ttgtctataa gcatcaccaa ttctatatgg attatttgaa
    10621 ggattatatt cagtatgtgt gagaccactt gatgtgaaat cgtagattgg atataagttg
    10681 gctggtgtgc gtttactgta ttcagcgtag tgcacatcac cacttaccac aaatgcacct
    10741 tcagcgttag catctctcaa agcttgatag aatttttcca tttcatgtgg gaaatttgcc
    10801 caagtttccc aaccattatg acccgttgca aattgagtac tagaaacaat aattctaatt
    10861 ttagctggtg tttgcaattg ctgtctgaac caagcccatt gcgtagctcc taagatagtt
    10921 ttagaagtat ctgttgttgg cgcaataggt tcgccgctta acacatctaa gaaatttcta
    10981 caatctaaca ggattacttg cacacgatga gcagcgtcgc catataagta tgatgtataa
    11041 acaccatcat gtgtgcgaat atcatgcgta gattggattt gccaaaaact catgaacttt
    11101 tctttagatt gcaatttgta aggaaattct tttccagcat tattcaaacc ataatcgtga
    11161 tcgtcccaag tggcaatact ttgtatagat tttctaaaat tagaataatc catattattg
    11221 cttaataagt tatactgaga ttgaatccaa ttgggataat caggattgct gatgatgtta
    11281 tcagcataca tgttatcgcc catagcaata tacaaatcag catctttggc ttgcatattt
    11341 ctaaaaattg aaagatttcc gcctgagtaa cagcaagaac taatcacaat tttatttata
    11401 attgtcgatg gatcataagc cggatacgtt ggaatatttc ccgtaggaat gcaagccgta
    11461 ataaatatta caggcaagct gagtaaatag aataattttt tcattggtgg attttcacca
    11521 aatatacaca ataagtacaa aatatcaatc cattacaatg gaatattgcc atgttttttc
    11581 ttaggtgaat tgactacttt attttctaac atctggaagg ctttaataag ttttattctt
    11641 gtctcttctg gtttgatgac ttcatcaata aaaccacgtg ctgccgcctt atatggatta
    11701 gcgaatgtgt ctgtatattc atctactttt tcttgcaatt tctgtgtagg attttcagct
    11761 tgtttgattt catttttaaa aataatctct gctgctcctt ttggtcccat aacggcaatt
    11821 tctgctgttg gccatgcaaa attcatgtct gcaccaatgt gcttagaatt cattacatca
    11881 tacgcgccac catacgcttt gcgtgtaatg acggtaactt ttggaactgt cgcttcgctt
    11941 aatgcatata ataactttgc tccattagta ataatgccat gccattcttg atctgtgcca
    12001 ggcaaaaatc caggtacatc aaccaacact aataaaggta tattaaaagc atcgcaaaag
    12061 cgtgtaaaac gcgctccttt cctactgctg ttgatgtcta aaacgcctgc taaataggca
    12121 ggttggttgg caacaattcc tatacttcta cctgctatac gagcaaatcc gactacaaga
    12181 ttttctgcaa aatttttgtg cacttcaaaa aatgaatttt catctgttgt gtgctgtatg
    12241 acttctcgaa tatcataagg ttgattagga tttgatggca atattgacga taaggcatct
    12301 cgtttttcag aaccttctgt ttgataagga taaaccggag ctgtgtcttc gcaattttgt
    12361 ggtaaatagc tcaacaattg ttttacctgt tgaatacatt ctacttcatt tttggctgta
    12421 aaatgtgtta caccactttt tgttgcatgt gtttgtgcac cgcctagctc ctcagaagtt
    12481 acttcttcgt gtgtcactgt ttttgctaca ttcgggcctg taacaaacat ataagatgta
    12541 ttttctacca ttaaagtaaa atccgtcatt gctggcgaat acacagcacc accagcacat
    12601 ggtcccataa tggcagaaat ttgtggcacc acgcctgaaa attgtacatt gcgatggaaa
    12661 atatctgcat agccaccaag cgaaactacg ccttcttgaa tacgtgcgcc accagagtca
    12721 tttaaaccaa caacaggtgc acctgttttt gcggccaaat ccattatttt acaaattttt
    12781 tcagcatgtg tttctgacaa tgagccacca aaaacagtaa aatcttgtga aaaaacatac
    12841 actaacctgc cattcacttt tccataacca gtaacaacac catcaccata atatttttct
    12901 ttatcgatgc caaaatcagt agctctgtgt gtgaccaaca tgcctaattc ttcaaacgta
    12961 cctgcatcta acaataattc tattcgttcg cgtgccgtca gttttccttt tttatgttga
    13021 tcgtcaatac gtttttgtcc accaccaagt aatgcttctt tcttcttaac ttctaatgct
    13081 tctaattttt gttcgtgtgt cataattaca aaaataaggt ttaatgctga atagcgtact
    13141 cacttcattc aaattaatca taagtttaaa aatctatctt gaatttatta tctaattgat
    13201 aggtaagttt tgttggcgcg cccactggtg gcgctgcatc ataatttaat ttgaaattca
    13261 ttccaaatga aagtattcta tgaaatttga ataaaatagc attttctgtg gcaatacgat
    13321 aatcttttat ctcatcaaat cttggttgat aataggtagt gctaataaac tctatcgttt
    13381 ttataggtcg tatactaaaa cttaaataag cactaaatcg accttgcagt agcttttgtg
    13441 acttatcgtt attaatttca tactcaagca tgtacaaagt ccctaaatac gttttgaatt
    13501 tttctatttg caccaactta aaccttggtc ctgtgcctaa taagtagcgc attcttaaac
    13561 caatcaactt attatattgc acttgtgtaa atgcttccca gcgcaaccaa tgttttattt
    13621 tataattata tctaaaatga aaaaaaccag cgttgacaaa cttctcattt ccttttttta
    13681 gaatatccaa gcttcctaaa agtaaataca agctccgctt agatttatat tgcacatgtg
    13741 cagaattgaa aaatgaaaaa tatttttctt tttgtttgcc aaatgtgaaa cctaaattgt
    13801 aatcgccagc ccaacctgtt gtatcagttg cataacgctg gctctcaata tttacgattt
    13861 gagcattgac tttattaata aatacaaaaa ctaaaaaaac aatcgataaa agcaggtttt
    13921 tcatagt
//
LOCUS       DAOQQV010000067        13812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1477_length_13812_cov_7.979283, whole genome shotgun sequence.
ACCESSION   DAOQQV010000067 DAOQQV010000000
VERSION     DAOQQV010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13812
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1477_length_13812_cov_7.979283"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLL59_07930"
     CDS             <1..663
                     /locus_tag="PLL59_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23776.1"
                     /translation="TNNNVTKLNGAIQYELIRPSFSTYRFHTNGGWGQPLPVEMVSFT
                     GFNEGIVNRLKWTTASEINTDRFEIEKSLDNSSWSLIGSKEAAGNSNQSLNYDFTDNE
                     PIVGNNYYRLKILDNDGTFKFSNIINIPISEAVINGFSRIYPNPTGGQLNVELQATTA
                     YETKITTYDVVGKKVQEKNISLVKGLNSLLFDFSDLAKGTYLLQFTDSKGKTHINKFV
                     KD"
     gene            complement(812..1552)
                     /locus_tag="PLL59_07935"
     CDS             complement(812..1552)
                     /locus_tag="PLL59_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HMY23777.1"
                     /translation="MSDCIVIIPTYNEKENIAQIVPYTLGLSERIDILIIEDGSPDGT
                     AAVVRQLMQAFPNRIHMIERSGKLGLGTAYITGFKWSLTKGYSYIFEMDADFSHPPEK
                     LLELLDACENRGAYLSVGSRYIKGGKVVNWPFDRLFLSWFASKYVRLVTGMRIKDTTA
                     GFVCYRKSTLQNIDFDKIKFVGYAFQIEMKFAVWLSELKVIEVPIEFKDREVGTSKMN
                     GGIIKEAVIGVLQMKWQAFKNKYFYVKQ"
     gene            complement(1664..3004)
                     /locus_tag="PLL59_07940"
     CDS             complement(1664..3004)
                     /locus_tag="PLL59_07940"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HMY23778.1"
                     /translation="MQHYHFIAIGGAVMHQLASHLKNQGNRITGSDDAIYEPAKSNLE
                     ALGLMPAQVGWFPEKIDSTIDAIILGMHAKADNPELLKAQSLGLKIYSFPEFVFEQCK
                     NKTRIAVAGSHGKTSITSMIMHMLRENYKDFDYLVGAKLEGFNFMVKTSENAPLMVIE
                     ADEYLTSPLDLRSKFLHYHPHIAIISGIAWDHINVFPTFDEYLETFRKFIKSIIPGGY
                     LIYNEEDEELVKMITSLQLSIHLIPYKAFQFDTINDESVLLHQTAQYVINVFGSHNFA
                     NLKAAFEVGKIIDLNDNAILNSFQTFKGAAKRLEMIANDSEKRITIFRDFAHAPSKVK
                     ATVSAVRERYANRKIIAVFELHTYSSLQENFIEQYAHSLDAADVRILFLDEEALKIKN
                     KQDLDTHLLKQQFADDSIVVYKKMETLKEKIENEKTENCVLLLMSSGNLGGLQV"
     gene            3246..3392
                     /locus_tag="PLL59_07945"
     CDS             3246..3392
                     /locus_tag="PLL59_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23779.1"
                     /translation="MNKLTKSLLAVAFAGFILSLSSCTASHRGTGCPAWSKAKQEQIN
                     KKNV"
     gene            3398..3724
                     /gene="ytxJ"
                     /locus_tag="PLL59_07950"
     CDS             3398..3724
                     /gene="ytxJ"
                     /locus_tag="PLL59_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HMY23780.1"
                     /translation="MNWLALTAEAQFAEALKEQPLFGVFKHSTRCSISNMAKNRLERD
                     WRFDFPIYYLDLLNYRPISNLIADTSKVQHESPQLIVFQNGKAIYAASHNGIVMDDVQ
                     AAITKQ"
     gene            complement(3714..4124)
                     /locus_tag="PLL59_07955"
     CDS             complement(3714..4124)
                     /locus_tag="PLL59_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMY23781.1"
                     /translation="MTTAKVEYLGELRTEATHTQSASILITDAPIDNNGKGAAFSPTD
                     LTATSLASCMMTIMGIKVAAWQKSMVGTVATVKKVMYGEPRRIGEIHIHMQVPANDFT
                     DKEKIALIQAAHHCPVAKSLHPDILQQIEIEFIA"
     gene            4241..4975
                     /locus_tag="PLL59_07960"
     CDS             4241..4975
                     /locus_tag="PLL59_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23782.1"
                     /translation="MAQYRREDSNLNRDSVLYKKSWAAGLRLRSNGFGLGAEFTKSKR
                     YKRSLLFQVQFSYFIHPKQSRQQSQYGGGGFFGDGFKPFVYGKQNSLFTLYGGVGQKF
                     LLAEKGKRHGVQIFVKYAGGFTLALLKPYSLRVIPKPLTSADIEDLVDVTYEEGVDNS
                     FLDYDRIVGASGFGKGWKIKVLPALHLDVGFDFDFGKNESIVKTLEIGIIADFYYKKV
                     PVMVQDNKFLYPGVYVGFMMGKRKEK"
     gene            4992..5876
                     /gene="lipA"
                     /locus_tag="PLL59_07965"
                     /pseudo
     CDS             4992..5876
                     /gene="lipA"
                     /locus_tag="PLL59_07965"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            complement(5919..6449)
                     /locus_tag="PLL59_07970"
     CDS             complement(5919..6449)
                     /locus_tag="PLL59_07970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HMY23783.1"
                     /translation="MTEQLRIRQETPDDFKEIFEVNHIAFGQDNEAKLVDALRSNPTL
                     FIPELSLVATVNNKIVGHILITKIIIKDNNGNLNESLGLAPMAVRPEFQKSGIGGQLI
                     KQGLKVAAELGFKSVIVLGHKHYYPKFGFEPAEKWNIKAPFDVPSDVFMAIELVKDGL
                     KNVSGTVIYPKEFETV"
     gene            complement(6518..6823)
                     /locus_tag="PLL59_07975"
     CDS             complement(6518..6823)
                     /locus_tag="PLL59_07975"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMY23784.1"
                     /translation="MNKKIIKNAKNFDELLDIKYGKIGSENRDKFEEKAQYFVISEML
                     KEARREANMTQEQLAEKVGTKKSYISRLENGKCDIQLSTLYRIFEFGLGKRVNLLFG"
     gene            complement(6823..7170)
                     /locus_tag="PLL59_07980"
     CDS             complement(6823..7170)
                     /locus_tag="PLL59_07980"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMY23785.1"
                     /translation="MNTERQIIFYEHYFIDFYVEQSEKVQEKIEYVFKILRTVQNVPK
                     KFLDHITGTDGLYEIRIEFESNIYRIFCCFDKGNLVVLFNGFQKKSQKTPKKEIDLAL
                     KLKDGYFNSKKTK"
     gene            complement(7470..7913)
                     /locus_tag="PLL59_07985"
     CDS             complement(7470..7913)
                     /locus_tag="PLL59_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23786.1"
                     /translation="MGTIFQICCDIIRALSNFWGITYEEMNTYLFIYAQPILSILSFV
                     TTISIITSKLLKKVSMLNLFNLVLFGMGGLVYTITTFVVWKRYSVLSAHDTCILAYKD
                     LETLGKMTGMGYIGINFFLFIILFSLVFGFNIISAFLIKKYVKVN"
     gene            complement(8105..9349)
                     /locus_tag="PLL59_07990"
     CDS             complement(8105..9349)
                     /locus_tag="PLL59_07990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMY23787.1"
                     /translation="MKFSDYHINEDIKYNLDKLGFKKPTDIQFKSIPAILRGEDVLAI
                     AQTGTGKTAAFAIPLIHKIHQDKTSKRTDYIKCLVMVPTHELAIQIAEVFEKIAEKTR
                     VKIATIFGGVEQEKQIQTLYKGVDIVIATPGRMFDLQHQGALDVSKIDILVLDEADHM
                     LDLGFIDDIRDMMRFVPKKRQTLFFSATINEKIKKLAYSLVSNAIRIQISPKDPVSKN
                     VQHAVVFIEMDDKRFFLERFINEHADNKILVFVRTKVRAERVMNAMARANIKTLTMHS
                     DKAHEERLKVMHDFKSGKAKVLIATDINARGIDIPNVDYVVNYDLPDEPENYVHRVGR
                     TGRGDKKGQAISFCSTEEKPTLKIIETFLHKPIHIIDIEKGDYMETIEFANDTIQKED
                     LQSLLKEAEAFEILQKKKKKKK"
     gene            complement(9426..9998)
                     /locus_tag="PLL59_07995"
     CDS             complement(9426..9998)
                     /locus_tag="PLL59_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY23788.1"
                     /translation="MLQIQLKEFPVLATNRLILRQICEIDFMEFYWLRNSDVVMQYIQ
                     LPKFKEEEEAKQKIRDLINICNKNEGFHWAITLRTDPTLIGWILLKDIDLTNHRAEIG
                     YLLHPDFWRKGIVQEAILAVIDFSFTTLQLHSLEAHVHPENIASFKLLEKVGFVKEAH
                     FKENFYFEGNFLDTAVYSLLKSRFNSNILR"
     gene            complement(10015..10770)
                     /locus_tag="PLL59_08000"
     CDS             complement(10015..10770)
                     /locus_tag="PLL59_08000"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY23789.1"
                     /translation="MNNELIKKYLADELSPIERNAFEQEMENDPFLKEAVEGLAMLSK
                     KMPKQQLHELEEKLKDNIDTTIRASSQRKRFTNVFFKYAAAACLIGVLGLLSYNLFFI
                     EKTINEQEIFANYYKPLTHPDATLRGDTKTNAETKAIQAYENENYFEAVNAYQKLVDE
                     NPDNVKNNLFLGISYLSTNQPKKAITHLKSITASEEFHYDIQWYLALAYLKNKNIEQA
                     QSIIYHLAHEENYYQKEAKEILEKLDGKIALKE"
     gene            complement(10760..11332)
                     /locus_tag="PLL59_08005"
     CDS             complement(10760..11332)
                     /locus_tag="PLL59_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMY23790.1"
                     /translation="MTNIYAQYSDAELITLYQKELQADIVGELYKRYALLVYGLCFKY
                     LNDEGEAEDAVSEIFELLLSKLQVQEINFFKSWLFIVSKNHLIRKQKKKNDIKTVEIE
                     KISENFMENEEDVSLNIRDEQKELLRQAISNLNEEQRTCIELFYYQQKPYQEVASISG
                     YDLNKVKSAIQNGKRMIKLFMEENYSQHEQ"
     gene            11416..12225
                     /locus_tag="PLL59_08010"
     CDS             11416..12225
                     /locus_tag="PLL59_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23791.1"
                     /translation="MEYFQIVVFLKVRFFLYILIACLPAIVQAQSAEIVVNVKIVDEN
                     NNIVPYADIAFRRLQLGFSASKEGFFSTKMLKSDSLIILKKGHVPSKLSCKDSVLRAS
                     YDIVFVLPRIPVELTEVQISAIRTHQQIRDQINNLYIKNTAKNPDARPFTNPISYLYQ
                     LLSKHEKEKKVAAQLETEEAKRIVLKDLFRLYNNYHIIDLDEDEYDRFISYLNMPYTF
                     LQQTSDYDLAVNIKRMYAGYNKDKSSSFRKQIYPAALDDMEFIKHKPTDER"
     gene            12250..13494
                     /locus_tag="PLL59_08015"
     CDS             12250..13494
                     /locus_tag="PLL59_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23792.1"
                     /translation="MKQFFSVLLTSIFFHTAFAQCEGGRFTQKIFQQSIRHKDIVYSI
                     AKNSAGLLQPIKMDVFEPVNDTATVRPLVIFEHGGAYWTGTKDYESQIAMGNEFAKRG
                     FVVCSATYRLESSPLSLLFQDLMLKAVGRGVQDTKQLIRYFFNSARDSANPYRIDTSK
                     IFLCGASAGAFNVLHTTYLDSTDVLPENWKTWLNDIGGVFGEYDFINYGEKILGVVNI
                     NGALGDKTYLDNEHTNFLSVHNLWDPEIPFNRGQPYNIPTLMFVDGSNILHTKAQELN
                     IYNPFYIIPDWGHTSYSTDLFGTVVQPFFDSTVWYMKNFFAYQLGCPNLTTNLNYFQT
                     IEKIGVYPNPTFDNIYFENVQRYVGQQITISDILGNKYYDAIFDGNVLNTSALGMTKG
                     VYTIQLYNRKTTTLYTSKIIVD"
     gene            13670..>13812
                     /locus_tag="PLL59_08020"
     CDS             13670..>13812
                     /locus_tag="PLL59_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HMY23793.1"
                     /translation="MAKTKLSIQPLADRVVVKAAAAETKTKSGIIIPDSAKEKPQKGE
                     VVAV"
BASE COUNT         4401 a         2353 c         2282 g         4776 t
ORIGIN      
        1 actaacaata atgttacgaa acttaatggc gctatacaat atgaattgat acgcccatcg
       61 ttttctacgt atcgctttca taccaacggc ggctggggac aacccttgcc ggtagaaatg
      121 gtatcgttta ctggctttaa tgaaggaatt gttaatcgtt tgaaatggac aacagcttct
      181 gaaatcaaca ctgatagatt tgaaatcgag aaaagtttgg ataactctag ttggagttta
      241 ataggctcaa aagaagctgc tggtaattct aaccaaagtc taaactatga ttttacagat
      301 aacgaaccta ttgttggcaa taattattat cgccttaaaa ttttggataa tgatggtact
      361 ttcaaattca gtaatatcat taacatacca atatctgagg ctgtaataaa tggattttcc
      421 agaatctatc caaatccgac aggtggtcag ctgaacgtag agttgcaagc aactactgct
      481 tatgaaacta aaattactac ttatgatgta gttggtaaaa aagtacaaga gaaaaacatc
      541 agtttagtta aaggattaaa tagcttgtta tttgattttt ctgacttggc aaaaggaact
      601 tacttgctac aatttactga cagtaaaggt aaaacacaca tcaataaatt tgtaaaagat
      661 taaaaacaaa caatttatta gcaaaaaacc gactccaacg agtcggtttt ttttgaatga
      721 tatcacaatt taagtggaag ataagtttta tttcaaatgt cattcctaag aaagcgggaa
      781 ccttaagcta atctgcatca cattaccaga attactgttt cacataaaag tacttgtttt
      841 taaatgcttg ccatttcatc tgaagcacac cgatgacagc ttccttgata atgccgccat
      901 tcatttttga tgtgccaact tctcggtctt taaactcaat tggtacttca attactttca
      961 attcagatag ccaaacagca aatttcattt cgatttgaaa ggcatagcct acgaatttta
     1021 ttttgtcaaa atcgatattt tgtaatgttg attttctata acatacaaaa ccagcagtag
     1081 tgtctttgat gcgcatgcct gttactaaac gtacatactt agatgcaaac cacgataaaa
     1141 acaaacgatc aaatggccaa ttaacaacct tgccaccttt tatatatcgc gaaccgacag
     1201 acaagtaggc accacggttt tcacaagcat cgagtagttc tagcaacttc tctggtggat
     1261 gtgaaaaatc tgcgtccatt tcaaaaatat aggaatatcc ttttgttaat gaccacttaa
     1321 atccagtgat gtaagccgtg cctaagccaa gctttccact gcgttctatc atgtgtatcc
     1381 tattgggaaa cgcttgcatt aattgtcgca caacagcagc tgtgccatcg ggcgagccat
     1441 cttcgatgat taatatatcg atgcgctcag ataagccaag tgtataagga acaatttgtg
     1501 ctatgttttc cttttcgtta taagttggta taattacaat acaatcgctc atctgttgca
     1561 acaaaaatac atagaaactt agttaaatag ttaaagtgtt aacgcttttt atagatttaa
     1621 taattggaca taaaatagta aaatgtgttt gaaaggcatt caactaaact tgtaaaccac
     1681 ctaaatttcc agagctcatc aataatagta cacaattctc agttttctcg ttttcgattt
     1741 tctctttgag cgtttccatc tttttataca caacaatact gtcatcggca aattgttgct
     1801 ttaacaagtg tgtgtctaaa tcttgtttgt ttttaatttt tagtgcttct tcatctaaaa
     1861 ataaaatgcg cacatcggca gcatcaagtg agtgtgcata ttgttcaata aagttttcct
     1921 gtaaggaact atatgtatgt aattcaaata cagcaataat tttccgattt gcatatcgct
     1981 ctcttactgc gctcactgtt gccttcacct tacttggcgc atgtgcaaaa tcgcgaaata
     2041 ttgttattcg tttttcgctg tcgttggcta tcatttccaa ccttttagcg gcacctttaa
     2101 aagtttgaaa gctgttcaat atggcattgt cgtttaggtc aataatttta ccaacttcaa
     2161 atgctgcttt aaggtttgcg aaattgtgtg agccaaaaac attaatcaca tattgtgcgg
     2221 tttgatgtaa taatacactt tcatcattta tagtgtcaaa ttggaatgct ttataaggta
     2281 ttagatgaat agatagttga agtgaagtaa tcatttttac taattcctca tcttcttcat
     2341 tataaataag atatcctcca ggtattatgc ttttgataaa cttcctaaat gtttctaaat
     2401 attcatcaaa agtcggaaat acattaatat gatcccaagc aattccagat attatggcaa
     2461 tatgtggatg ataatgaaga aatttacttc ttaaatctaa gggcgaagtt aaatactcat
     2521 ctgcttcaat aaccataagt ggcgcatttt cagatgtttt taccataaaa ttgaaacctt
     2581 caagtttagc tccaactaaa taatcaaaat ctttataatt ttcacgcaac atgtgcatta
     2641 tcatagatgt tatagatgtt tttccatgac tacctgcaac agcaatacgt gttttgtttt
     2701 tgcattgttc aaatacaaat tctggaaaag aataaatctt caaacccaat gattgtgctt
     2761 tcagtaattc aggattatcg gcttttgcgt gcattcctaa aataattgca tcaatagtac
     2821 tgtctatttt ttcaggaaac cagcctactt gcgcaggcat taatcctaaa gcttctaaat
     2881 tagattttgc tggttcataa atggcatcgt ctgaacctgt tatgcgattt ccttgatttt
     2941 ttagatgaga agcgagttgg tgcatgaccg ctccgccaat agcgataaag tgataatgtt
     3001 gcatactgta aaaatagaat tccgaaaatg gctagaacat aaattttata aacaagtgtc
     3061 aaatacaaaa acaaacgaaa acttcgcaag tgttttccaa tttttttccc ttttcacaat
     3121 tatacgacaa aagatatatt cacaaatgat ttttaaaatg ttaataaaag tgaagtagct
     3181 gtgcacaagt ataataaata cattattttt gcattcaact ctattacaac gtaaacaaca
     3241 aagacatgaa caaattaact aaatcacttt tagcggtagc attcgctggt tttattttaa
     3301 gcttatcatc gtgcactgct tctcacagag gtacaggttg ccctgcatgg tcaaaagcaa
     3361 agcaagaaca aattaacaaa aagaacgttt aagccttatg aattggttgg cattgactgc
     3421 tgaagcacaa tttgcggaag cattaaaaga acagccattg tttggcgtgt ttaaacatag
     3481 cacacgttgc tctatcagca atatggcaaa aaataggctg gagcgtgatt ggcggtttga
     3541 ttttccaatt tattatttgg atttgttaaa ctatagacca atttccaact tgattgcaga
     3601 tacatctaag gtgcaacatg aatcgccaca gttaattgtt tttcaaaatg gaaaagcaat
     3661 ttatgctgcg agccataacg gtattgtgat ggatgatgtg caagccgcaa taactaagca
     3721 ataaattcaa tttctatttg ctgtagaatg tcaggatgca aactcttagc aacgggacag
     3781 tgatgtgctg cttgaattaa tgctattttt tctttgtcag taaaatcatt ggcaggcact
     3841 tgcatgtgta tatgtatttc gccaattctt cttggttcgc catacatcac tttttttacc
     3901 gttgcaactg tgcctaccat acttttctgc caagcagcca cttttattcc cataatggtc
     3961 atcatgcatg acgctaatga agttgctgtt aagtctgtcg gcgaaaatgc cgcgccttta
     4021 ccattattgt caataggtgc atctgtaatt aatatgctag cactttgcgt gtgggtcgcc
     4081 tctgttctta gttcacctaa atattcaact tttgccgttg tcatctaaaa atattttttt
     4141 acttttaaac taaattagtc aatttagcgt cataatggaa gtgaaacgaa ttttttctat
     4201 acttttttta cttttaggcc tattttcatt ttctgagagt atggcacagt accggcgcga
     4261 agactctaat ctcaatagag attctgtgtt gtataaaaaa tcatgggcag ctggcttacg
     4321 cttgcgtagc aatggttttg gacttggcgc cgagtttact aaaagtaaaa gatacaaacg
     4381 tagtttgctt tttcaagtcc aattttccta tttcattcat cctaaacaat cgcgccaaca
     4441 atcacaatat ggtggaggcg gtttttttgg cgatggattt aagccttttg tgtatggtaa
     4501 acaaaattct ttatttacac tatatggcgg agtaggtcag aaatttttat tagcagaaaa
     4561 aggcaaacgg catggtgtgc aaatttttgt aaaatatgct ggcggtttta cattggcttt
     4621 attaaaacct tattccttgc gcgtgattcc aaaaccactt acctcagctg atattgaaga
     4681 cttagttgat gtcacctatg aagaaggtgt agataatagc ttcttagatt atgatagaat
     4741 cgttggcgct tctggatttg gcaaaggctg gaaaataaaa gtgctgccgg ctttgcatct
     4801 cgacgtcgga tttgattttg attttggcaa aaatgaaagc attgtaaaaa ccttagaaat
     4861 cggaattatt gctgatttct attataaaaa agtaccagtc atggtgcaag acaataaatt
     4921 tttatatcca ggtgtttatg tcggttttat gatgggaaaa agaaaagaga aatagaaaat
     4981 caacatacac acaattatca aaatatttca tgaacgatag tatagaaacg atatcaccaa
     5041 aaccacgcaa gccggaatgg ttaaaagtaa aaattccaat aggtgagaac tataaacgtg
     5101 tgcgaaattt agtggacacg cacaaactgc acaccatttg tgaaagtgga aattgcccaa
     5161 acatgggcga atgttggggt gccggcactg caacatttat gatcttagga aatgtatgca
     5221 cacgatcttg ttctttttgt gctgttgcca caggccgacc taaatttgtc gattatgacg
     5281 aaccgaaacg tgttgctgaa gccattcatc taatgcaagt aaaacatgca gttatcactt
     5341 ccgtaaacag agatgaaatg ccagacaaag gcgctcagat ttggcatgat acagtaagat
     5401 tagttaaaga aatgtcgcca gcaacaacta tcgaaacatt aattccagat acaaaaggcg
     5461 attggcaagc attagaaata atgattagcg cagggcaaga agttgtttcg cataatatgg
     5521 aaactgtaaa aagcttgtat cgtaaagtgc gaccacaagc aaagtacgaa agaagtttag
     5581 agcaaataca acgaaccaaa gattatggca aacgcacaaa aagtggcatc atgctgggct
     5641 ttggcgaaac agaagaagaa gtgtatgaaa tcttacacga tttaaaaaat catggttgcg
     5701 atgtggtgac attaggtcaa tatttacaac ctactaaaat gcatatcgaa gtagcagaat
     5761 ttgtgcatcc aactatattt gcaaaatata aagaatatgg tttgtctatt ggcattccgt
     5821 atgtagaaag cggacccatg gtgcgttcat cataccatgc agaacgacat atttaaagac
     5881 aatgtctttg ctcttatttt tcatttgtgt ttgttacgtt aaactgtttc aaattctttt
     5941 ggataaataa ctgttcccga tacatttttc agtccatctt ttactagttc aatcgccata
     6001 aaaacatcag aaggaacgtc aaacggtgct ttaatattcc acttttcggc tggttcaaat
     6061 ccaaattttg ggtaataatg tttgtgccct aatacgatta ccgatttaaa gcctaattct
     6121 gctgctactt tgagtccttg tttgattagt tgtccaccaa ttccactttt ttgaaattca
     6181 ggtctaacag ccattggtgc aagtcctaaa ctttcattta agtttccgtt attgtctttg
     6241 attatgattt tcgtaattaa gatatgccca acgattttat tgttgacagt tgccacaagt
     6301 gaaagttctg gaataaatag ggttggattg cttcttaaag catcaactaa ttttgcttca
     6361 ttgtcttgtc cgaatgcgat atgattgact tcaaaaattt ctttaaagtc gtctggtgtt
     6421 tcttgtctta tccttagctg ttctgtcatt ttatttctgt ttatggtatt tatttttaga
     6481 gtaaggcata acgttttgct agttgtaggt tttattttta tccaaatagt aagtttactc
     6541 gttttccaag tccaaattcg aaaattctat aaagagtaga aagctgaata tcacattttc
     6601 cattttcaag tcgagaaatg taacttttct ttgttccaac tttctcagca agttgctctt
     6661 gtgtcatatt tgcttctcta cgggcttctt tcaacatttc actaattaca aaatattgtg
     6721 ctttttcttc aaatttgtct ctattttcag accctatttt tccgtattta atgtctaata
     6781 actcgtcaaa gtttttagca tttttgataa tttttttgtt cattactttg ttttttttga
     6841 gttaaaatat ccatctttta atttcaatgc caaatcaatt tcttttttag gtgttttttg
     6901 agatttcttt tgaaaaccat tgaatagaac gactaaatta cctttgtcaa agcaacaaaa
     6961 aattctatat atgttgcttt caaattctat tctgatttca taaagcccat cagttcctgt
     7021 tatatgatcg agaaactttt taggaacatt ttgtacagtt ctcaaaatct taaaaacata
     7081 ctcgattttt tcttgaacct tttcactttg ttcgacataa aaatcaataa aataatgttc
     7141 gtaaaaaatg atttgtcttt ccgtattcac ttcacaaagt taacttaaaa gttatcagta
     7201 tgcaagtttt attttattga attttgaaat gtgtgttcgg gaatgcttgt cgcttcgttt
     7261 tggcgacttg acgaggtaga gcattttagt attaccgttg atgcgagaac tacagctcaa
     7321 ttttagcaca aaagttcgaa cggttcaatt cacgcatgct tgtctttaaa ccgattctgc
     7381 ctgctagctg tcctttcatt atacttatat aaatatttat ttaggtgttg acattctttt
     7441 acccatgttg aaaaatcaat tgtaaaagcc taatttactt tcacatactt cttgattaag
     7501 aaagcagaaa ttatattgaa gccgaataca agcgaaaata aaataataaa caaaaaaaaa
     7561 ttaattccaa tataacccat tcctgtcatt ttccccaaag tttccaaatc tttataagcc
     7621 aaaatacaag tatcgtgtgc ggataaaacg gaataacgtt tccatacaac aaaagttgta
     7681 attgtatata ctaaaccacc cattccaaat aatactaaat taaaaagatt aagcatagaa
     7741 acctttttta acagcttgga tgtaataata cttattgtag ttacaaatga aagtattgat
     7801 agtataggtt gagcatagat gaataaataa gtattcattt cctcataagt tataccccaa
     7861 aaattagata aagctctgat aatatcacaa catatttgaa aaattgttcc cattgtttaa
     7921 tttttttact ctgtattctg aatctattcc aatgcacgtt ggtgaaaaat ggaaataaat
     7981 tcagcagttg tgtttggttt agtttttcaa aattccgatt tatgactgct gttcaaatca
     8041 gcttaccact tactattaaa aatagttaca aagtgtcaaa aaatacattt cattcaatat
     8101 tacgctattt cttttttttc tttttctttt gtaatatttc aaaagcttcc gcttctttta
     8161 gtaaagattg taagtcttct ttttgaatag tgtcattagc aaattcaatg gtttccatat
     8221 aatcgccttt ttcgatatca atgatatgaa ttggtttatg taagaatgtt tcaataattt
     8281 ttaaagtagg tttttcttct gtactacaaa acgaaattgc ttgccctttt ttgtcgccac
     8341 gtccagttct gcccacgcga tgtacataat tttcaggctc gtcaggtaag tcgtaattta
     8401 ctacataatc aacattgggt atgtcgatgc cacgtgcatt gatatctgtt gcaatgagta
     8461 cctttgcttt tccagattta aaatcatgca ttactttcaa tcgttcttca tgtgctttgt
     8521 cgctgtgcat ggtcagcgtt ttaatatttg ctcgtgccat tgcattcatc acccgttccg
     8581 cacgtacttt agtacgcaca aatactaata ttttattatc agcatgttca ttaataaatc
     8641 gctcaaggaa aaaacgttta tcgtccattt ctataaaaac gacagcatgt tgcacatttt
     8701 ttgaaacagg atctttaggt gaaatttgaa tgcgaatagc attgcttact aaggagtagg
     8761 cgagcttctt gattttttca tttatagttg ctgagaaaaa tagtgtttgt cgctttttag
     8821 gcacaaatcg catcatatct ctaatgtcat caataaatcc taaatcgagc atgtggtcag
     8881 cttcatctaa tacgagtata tcaatcttgc tgacatcgag tgcaccttga tgttgtaagt
     8941 cgaacattct gcctggagta gcaatgacaa tatcaacacc tttatataaa gtttgtattt
     9001 gcttctcttg ttctacgcca ccaaaaatcg tggcgatttt tacacgtgtt ttttctgcaa
     9061 ttttttcaaa tacttccgca atctgaatgg ctaattcgtg tgtaggcacc atcaccaaac
     9121 acttaatata atcagtacgt ttggaggttt tgtcttgatg gattttgtgt attaaaggaa
     9181 tagcaaatgc ggctgtttta ccagtgccag tttgtgcaat tgccaataca tcttcaccgc
     9241 gcagaattgc aggaatcgat ttgaattgta tgtctgtcgg ctttttaaaa cctaatttat
     9301 ctaagttata ctttatatct tcgttgatat ggtaatctga aaatttcatt acgttcttta
     9361 taatacaaag gtacgtagag aaacacagag tttccttttt tatttttcaa attgtgctga
     9421 ccgttttatc gaagtatatt actattaaaa cgcgatttta gcaaagaata gacagcagta
     9481 tctaagaaat taccttcgaa ataaaaattc tctttaaaat gggcttcttt tacaaatccg
     9541 accttttcca atagtttaaa tgaagctata ttttctggat gaacatgagc ttccaagctg
     9601 tgtaattgaa gtgtagtaaa gctaaaatcg atgactgcta atatggcttc ttgcacgatg
     9661 cctttccgcc aaaaatctgg atgcaataaa taaccaattt ccgcgcgatg attagttaaa
     9721 tcaatatctt tcaacaatat ccaaccaatt agagtagggt ctgttcgtaa agttattgcc
     9781 caatgaaagc cttcattttt attgcaaata tttattaaat ctcgaatttt ttgctttgct
     9841 tcttcttcct ctttaaattt tggcaattga atatattgca tgactacatc actattgcgc
     9901 agccaataaa attccataaa atcaatttca caaatctgcc gtaaaatcaa acgatttgtt
     9961 gcaagtactg gaaattcttt taactgtatt tgtagcataa tttaagcgta ctttttattc
    10021 tttcaatgca atttttccgt ctagtttttc taaaatttct ttagcctctt tttgataata
    10081 attctcttca tgtgctaaat gataaataat ggattgcgcc tgttcgatat ttttattttt
    10141 tagatatgct aatgctaaat accattgtat gtcgtaatga aattcttcag aagcagtaat
    10201 gctttttaaa tgtgtaatgg ctttcttagg ctggttggtg cttaaataag aaatgcctaa
    10261 gaataaatta ttttttacat tatcaggatt ttcgtcaact agtttttggt aagcatttac
    10321 tgcttcaaaa tagttttcat tttcatacgc ttgaattgct ttagtttctg catttgtttt
    10381 ggtgtcgccg cgtaaagttg catcaggatg tgtcaaaggt ttataatagt tggcaaatat
    10441 ttcttgttcg tttatagttt tttcaataaa aaataaattg taagacagta aacctaacac
    10501 accaattaaa caagcagccg cggcatattt aaaaaaaaca ttcgtaaatc ttttgcgctg
    10561 tgatgatgca cgtatcgtag tgtcgatatt gtccttgagt ttttcttcta attcgtggag
    10621 ttgttgttta ggcattttct tgcttagcat agccaagcct tcaacggctt cctttaaaaa
    10681 tggatcgttt tccatttctt gctcaaaagc attgcgctca ataggcgata gctcatcagc
    10741 caaatatttt ttaattagct cattgttcat gttgtgaata attttcttcc ataaataatt
    10801 taatcattct ctttccattc tgaatagcac tctttacttt attcagatcg taaccgctta
    10861 tggaagcaac ttcttggtaa ggtttctgct ggtaataaaa caattcaatg cacgtacgct
    10921 gctcttcgtt taagttgctt atcgcttgac gtagcagttc tttttgctcg tctctgatat
    10981 tgagagacac gtcttcctca ttttccataa aattttcaga aattttttca atttctactg
    11041 tttttatgtc attttttttc ttttgcttgc gaattaagtg attttttgac acaataaaca
    11101 accatgattt aaagaaatta atctcttgaa cttgtaattt actaagcaag agctcaaaaa
    11161 tttctgacac cgcatcttcc gcttcgcctt catcattcag atatttaaaa cacaaaccgt
    11221 ataccaataa agcatagcgc ttatataact cgccaacgat atctgcttgt aattcctttt
    11281 gatataaagt gattagttca gcatctgaat attgggcata tatgtttgtc attatatcta
    11341 aaattaaaga aatatttatt aaaatcaaac atttatacca aaatacataa caacataaat
    11401 taacatgtaa tgtaaatgga atattttcaa attgtcgtat ttttgaaggt gcgatttttt
    11461 ctatacatac ttattgcttg tttgcccgct attgttcaag ctcaatcggc agaaattgta
    11521 gtcaatgtaa aaatagttga tgaaaacaat aatattgttc cttatgcaga tattgcattt
    11581 cgacgtttac aattaggatt ttctgcttct aaagaaggtt tctttagcac aaaaatgctg
    11641 aaatcagatt cattgattat acttaaaaaa ggacatgtgc cgagcaaact ttcttgtaaa
    11701 gattctgtat tgcgcgcatc gtacgatatt gtttttgtgc ttccgagaat acctgtagaa
    11761 ttaacagaag tgcaaattag tgccattcgt acacatcaac aaataagaga ccaaatcaat
    11821 aatttatata ttaaaaatac agcgaaaaat cctgacgcaa gaccattcac taatccgatt
    11881 tcttatttgt atcaattatt aagtaaacac gaaaaagaaa agaaagtagc cgcacaattg
    11941 gaaactgaag aagccaaacg tattgtttta aaagacttat tccgtttgta taataattat
    12001 catatcatcg atttagatga agacgagtat gatagattta tttcctatct caatatgcct
    12061 tatacctttt tacaacaaac cagcgattat gatttggcgg taaatattaa acgcatgtat
    12121 gcaggttata acaaagataa aagcagttct ttccgtaaac aaatttatcc tgccgcattg
    12181 gacgatatgg aatttataaa acacaagcca acagatgaac gctaattgct ttttttgtat
    12241 ctttaattag tgaagcaatt tttttctgta ttattgacat caatattctt tcatactgct
    12301 ttcgcgcaat gcgaaggtgg acgatttacg cagaaaatat ttcaacaaag tattcgccat
    12361 aaagacattg tttattccat agcaaaaaat tcagccggct tattgcagcc aattaaaatg
    12421 gatgtgtttg aacctgtaaa tgataccgcc actgtaaggc cattagtgat atttgaacat
    12481 ggcggtgcat attggacagg taccaaagat tatgagagtc agatagcaat gggcaacgaa
    12541 ttcgctaaac gtggttttgt cgtttgctca gcaacctatc gcttagaatc ctcaccattg
    12601 tcattactgt ttcaagattt aatgttgaaa gccgtcggaa gaggtgtgca agatacgaaa
    12661 caactaattc gctatttttt caattccgca cgcgatagcg cgaatccata tcgtatcgat
    12721 actagcaaaa tatttttgtg tggcgcaagt gctggtgctt ttaatgtgct acatacgact
    12781 tatttagatt ctactgatgt tttgccagaa aattggaaaa cttggctgaa tgatattggt
    12841 ggtgtttttg gtgagtatga ttttataaat tatggcgaaa aaattcttgg cgttgtaaat
    12901 ataaatggag cattaggcga taaaacttat ttagataatg aacatactaa tttcttaagt
    12961 gtgcataatc tttgggatcc tgaaatacca tttaatagag gacaacctta taatattcca
    13021 actttaatgt ttgtagatgg tagtaatata ttgcacacga aagcgcaaga attaaatatt
    13081 tacaatcctt tttatattat tcctgattgg ggacatactt cgtattctac cgatcttttt
    13141 ggtactgtcg tacaaccttt ttttgatagt acagtttggt atatgaaaaa cttttttgcc
    13201 tatcaattag gctgtccaaa tctgactaca aatttgaatt attttcaaac cattgaaaaa
    13261 attggtgttt atccgaatcc tacctttgat aatatttact ttgaaaatgt acagcgatat
    13321 gttggacaac aaattacgat aagcgatatt ttaggtaaca aatattatga cgccattttt
    13381 gatggcaatg tattgaatac atctgcatta ggtatgacaa aaggagtgta cacaatccaa
    13441 ctctacaatc gtaaaacaac aacactttac acgagtaaaa ttatagtaga ttagagtgta
    13501 ttatatttaa tatattaaaa tacatgattg cctatttttt atattttgaa attccgagat
    13561 aactgaaaaa attgcattta atatggacat tttgacgttt ttatggaaat ggcacgaaaa
    13621 tagactactt ttgcgtgaaa attataatta attaaatcaa taaataatca tggcaaagac
    13681 aaaacttagc attcaaccct tagctgatag agtagtggtg aaggctgctg cagcagaaac
    13741 aaaaacaaaa tcaggcatta tcattcctga ttcagcaaaa gaaaaaccac aaaaaggtga
    13801 agtagtagca gt
//
LOCUS       DAOQQV010000068        13657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1503_length_13657_cov_8.144979, whole genome shotgun sequence.
ACCESSION   DAOQQV010000068 DAOQQV010000000
VERSION     DAOQQV010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13657
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1503_length_13657_cov_8.144979"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PLL59_08025"
     CDS             <1..1342
                     /locus_tag="PLL59_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HMY23794.1"
                     /translation="GHKDFAEDTYRTLTAVDSVILVVDCVKGVEMQTEKLMEVCRMRN
                     TPVIVFINKLDRDGKDPFDLLDELEQKLFIKVRPLSWPIGGGKLFKGVYNLFDKRLNL
                     FRASSTKVEEEFVSIDNIQDAELDKQIGERDAAKLREDVELIDGVYETFNEEKYKTGE
                     IAPVFFGSGVNNFGVRELLETFIRIAPSPRGRNTDTRVVMPTEEKMTGFVFKIHANID
                     PNHRSRIAFLRVCSGKFERNKYFYHTRLKKELRFSNPVTFMASDKNIVEDAYPGDVIG
                     MNDTGNFKIGDTLTEGENMMFKGIPSFSPEIFKELVNTDPMKTKQLEKGIQQLTEEGV
                     AQLFTVHTTQAKIIGTVGELQFEVIQYRLLNEYGASCQFKPLQYYKACWLTAENQTKL
                     NEFIRTKSNYIAFDKEKQPVFLASSEWILRLTQQDYPEIHFHLTSEFNKDEVVV"
     gene            1408..1845
                     /locus_tag="PLL59_08030"
     CDS             1408..1845
                     /locus_tag="PLL59_08030"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMY23795.1"
                     /translation="MNKRIDFLRSQIDKEQWQYDYPLMDWLRARVVAVDEGYVKMQFT
                     VEKYMLNPIGIMHGGIMATVLDEVMGAASFTLGRPNGFATVNMNVDYLNPAKLGETIF
                     AIGKIVRAGKTILHIEASIVNANDKLLAKATSNMIVTAVQLPI"
     gene            complement(2061..2294)
                     /locus_tag="PLL59_08035"
     CDS             complement(2061..2294)
                     /locus_tag="PLL59_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23796.1"
                     /translation="MGIIVTIILALLKIVDPTGGNIVDPTGGNLTVNQRIVDPTGGNI
                     VDPTGGNFTVSSTASARIVDPTGGNIVDPTGGN"
     gene            2763..4259
                     /locus_tag="PLL59_08040"
     CDS             2763..4259
                     /locus_tag="PLL59_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23797.1"
                     /translation="MDTLKELVTIVNRKRLSKIDVFDKALLSQSASNLYYKLYEGLES
                     GKITDDASAASYIYGSDDKDAKYRKLKSRFKNKLQKTLLLLDTEEVFINNQSRAYYEC
                     LLNSQIIEIIIKITGTTKLVYELAKENYSKAEQYNFNEILKNYSYYLLSYYAIIGDQK
                     SFSKEEQLYLKYLDLSQKEQYAKFLYFKASIKFENQAPITDALIEEVRVDVEALNKLR
                     LELQNIEIDFYYFFLALDFYQRSNQINKLIEVSNSAEDIMTLKPSAFTYTRKTIIYIY
                     KLAALLSNKQYDEVKNIFNNIEQLNVPEGNYNWYLLQDIHFKTYLQCNDIENATKIYE
                     TVVNGKSFKRQNEKVQEKWIIYHAYLVFMDNYIHHGDYKFSLAKFINTVPVNSRDKSG
                     FNFAIRIIEILFNAARNDFELIFLKMDALRIYKSRYLNDNTYKRNHILLNLLLKAEKS
                     GFSHKVLVNADWKEITDIRKQNANIVAEWEIMPYEILWDIFVEFTKKE"
     gene            4325..5557
                     /locus_tag="PLL59_08045"
     CDS             4325..5557
                     /locus_tag="PLL59_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMY23798.1"
                     /translation="MINTELFIAKRLALGDKNSFSRFIIRIAVVAIALSVTVMIVSTC
                     MVSGFSKEIKERIFGFWGQVHITNFANNESLETMPIKKEQVLVDKIRNAKDVKNIAPF
                     ITKAGILKTKTDIEGIALKGINKEYDWKTIQSYIKQGNIIAYNDSSASKDILISQSTA
                     KRLHLQVGDKVIAYFIRKELAAPIGRKLQVCGIFHTGLEEYDKQYALVDMELLRTLNQ
                     WQANEVAGYEIRLHDMNKMDAFKDSVYYKYVDQSVNAQTMKDINRNIFEWLDLQKVNE
                     YLIVGFMVIVAMLNMITALIILILDRTNMIGILKALGANSKSITNIFLYNASYIILNG
                     LFWGNLFGIGLCLLQRFTGFIKLNEENYYLSVAPVDFNIIAILLINAGTLVICTLVLL
                     LPTQLINKISPIKAIRFE"
     gene            5605..6666
                     /locus_tag="PLL59_08050"
     CDS             5605..6666
                     /locus_tag="PLL59_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HMY23799.1"
                     /translation="MFQKLLKWKWILLAFICYLLLMVLPASFVEKYYSNAFFLFVRNL
                     LDSSIAKIPFPAIYIFIVIVFLLLLKWFLHFFKEKPQPFFQRIFSLTSSIAAFIVFFF
                     VLWGFNYGRIPIEEKLNINPVTISNIQLLEETKFTIKQLENLRKTLKRDTGSLPQIIF
                     INNIETISRDALNKTLQNFSYPYSSKIRGRFVVEDMFLVFSIGGQYLPYVGEANVDDA
                     VFFSKKPFYLIHELAHGNGFTEEATCNFLAYVSCKQSGNVALEYSGELNYLLYLLSEL
                     KYRDSVAFDSLMLSLPHNIAKDLAEIKQHIAEHTFKTAFFGDLVNNLYLKILGIPDGV
                     KNYDKMLLLVYAWKHQYPK"
     gene            6837..7442
                     /locus_tag="PLL59_08055"
     CDS             6837..7442
                     /locus_tag="PLL59_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMY23800.1"
                     /translation="MPNKERRRKEKEELQQKILAVAKDIAASDGWQNVTIRKICDEIH
                     YTAPVIYQYFENKEAILQSLRTDGFIQVFNLFEKVNAKHQTADKRLLEYAITWWNFAI
                     QHPEIYQVMFNLHGAVCVHNDSNIKVHNTVMDYYHPAFKQINQKAKRSEIYSLELTDS
                     FIAIIHGFISMNMANKIKSGNNKATQVFKNSIIRFIHSLKE"
     gene            7453..8538
                     /locus_tag="PLL59_08060"
     CDS             7453..8538
                     /locus_tag="PLL59_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMY23801.1"
                     /translation="MKIGKIIFWTSIVLLIAGLAYIKHTNNKKAAAKATPFGKFGPST
                     LRATGYVAEYTKLSNMLSANGNILAQDEIQLQPEVSGRVTYLNINEGSVVAKGTLLMK
                     INDADLKAQVAKLNTQQKIAQTNFDRVAALLAIKGISQAEYDAAENTLNNIKADIQLL
                     QVQIDKTELRAPFTGKLGLRKISLGAYVSPNTIIASLQNVSQLKIDVSVPEKYAGIIH
                     VGDNMQCKVAGFDEPFTAKVIAVEPFIDDITRNLTIRAIIQQHNAKLIPGAYAKVELK
                     LKDSPNSIMIPSSAIIPDDRFTKVVVTDSSKAKFVNVEIGVRTESEVQIISGINIGDT
                     VLTSGLLQVKPGMSVQITKATTRSTIK"
     gene            8583..11645
                     /locus_tag="PLL59_08065"
     CDS             8583..11645
                     /locus_tag="PLL59_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMY23802.1"
                     /translation="MGLPSLSLRRPVFAIVINIIIILFGVIGYTFLGVREYPSLDPPV
                     INVRTSYTGANADIIESQITEPLENAINGVQGIRTISSASNQGSSNITVEFDLGADLE
                     TAANDVRDKVSQAIRLLPQDIDASPVVSKADANSDAIISMTIQSDSKNVLELTDYAVN
                     VLQERVQTIPGVSSIQIWGQKKYAMRLWFDPQKLAAYQLTAIDVQNALNKTNVELPSG
                     KIAGQNTELTVNTLGRINTPDEFNNMIIKDVKGKAVRLKDIGYATLGTENFETSLKNN
                     AVGMVALALVPQPGANYVEISDEFYKRLEKIKKEIPSDIKLDIALDNTVFVKNSILEV
                     KETLFIAIVLVVIIIYLFFRDWVIAIRPLIDIPVSLIGAFFIMYIFGFTINVLTLLAI
                     VLATGLVVDDGIVVTENIFKKLEAGMPKMQAAREGSEEIFFAVISTSITLAIVFLPIV
                     FMQGFVGSLFKEFGIVVAGAVLISAFVSLTLTPVLNVILGKKVHKNSWFYDKTEPFFK
                     GMEDGYRDMLNSFMKKRLIAIPIVVLCLALIYFIGKNLQSELAPMEDRGAFRTMMSAP
                     EGTSYTAMQDYVDKVSEFVIDSVPEQRLMLSVTSPGFTGSGAANSAFMRIGLKDKSER
                     TRSQDDIAQYLQRNFSKFNLGKAFVIQDQTISVGSARFGQPVQFVIQNFDFEKLQNTI
                     PKFMEEVSKSPVFIGADVNLKFNKPEVNVIIDKDKATALGVSVQDIAQTLQFSMSGRR
                     FGYFLKDGKQYQIIGQAQYEERNKPEDLKNIYVRNNAGALISLDNLIRIEELAKPPQL
                     YHYDRYKSATVSSGLAPGKTVGDGIAEMERIAKQVLDESFTTSLSGTSRDFAESSSNT
                     MFALILALVLIYLVLAAQFESFIDPFIIMMTVPLALAGALLSLWIFGQTLNIFSQIGM
                     IMLIGLVTKNGILIVEFANQSRAKGMGKSEAVAYAAVMRLRPILMTSLAMALGAVPIA
                     IAFGAGSTSRVSLGIVIIGGIMFSLILTLFVVPAIYSLLSRTTVKTVE"
     gene            11645..12952
                     /locus_tag="PLL59_08070"
     CDS             11645..12952
                     /locus_tag="PLL59_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMY23803.1"
                     /translation="MKLYRILLVSICLHSYVSLQAQTLLTPEQAVTTALQKNYDIVLA
                     KSEADIAKINNNKATAGMLPIINVTAGNVFNLNNINQKFTTGQEVKKNWVPVNSFTTG
                     LNMNWTIFDGMRMFAAKDRLAALQSLSEMQLNDQIQNTIAQVLSVYYEIVRQKQLIKA
                     LNESLKITEERVFLSNKKLEVGYADKTPLLQAKVDLSSQKINILKQETQLQQSKVQLN
                     QLLGRTANDLFDVIDTIEIKSALNALPTLDAIHQNNLLIKGANKNIEIAKLQHKEIKA
                     KRMPQINFNTSYNFMQNNSKAGLQLFNRSYGPQLGVQASIPIFNGGMVKKELQVSAVN
                     IAMKQIQVDQIKNSLDAQLQSAYNDFDYATKVLDLNEDNVKTAAENVQVTLERFRLSQ
                     STSLEIKQAQSSYEDALYNVVLARYNAKIAEIALQKLNNALPK"
     gene            complement(12956..13393)
                     /locus_tag="PLL59_08075"
     CDS             complement(12956..13393)
                     /locus_tag="PLL59_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23804.1"
                     /translation="MKAKENEIKFPIIQLLKIDSSSILTNDEIEDNKKTVFINFSPTC
                     DHCQRTIKSILDNIFKFTNTQFILSSFESLTSIRQFYFDYGLSSYTTVFIGQEIDYTL
                     TKQIQYSSFPCLILFDENKQFIKKIDEESDAKKLLKALHIKTK"
BASE COUNT         4808 a         2051 c         2384 g         4414 t
ORIGIN      
        1 aggtcacaaa gattttgctg aggatactta ccgaacgctt acagcagttg atagcgttat
       61 tttagtggtt gattgcgtaa aaggtgtgga aatgcagaca gagaaattaa tggaagtttg
      121 tcgaatgcga aacacaccag tcatcgtgtt tattaataaa ctcgaccgcg atggaaaaga
      181 tccatttgat ttgcttgatg agctggaaca aaaattattt attaaagtac gtccattatc
      241 atggccgata ggcggcggaa aattatttaa aggtgtatat aatttatttg ataaacgctt
      301 gaatttgttc agagcttcat cgacaaaagt agaagaggaa tttgtgtcta ttgataatat
      361 tcaagatgca gaactagata aacaaattgg cgaacgtgat gcagcaaaat tgcgagaaga
      421 tgttgaatta attgacggtg tttatgaaac atttaatgaa gaaaaataca aaactggtga
      481 aatagcacct gtgttttttg gctcaggagt caataatttc ggtgttcgag agctcttgga
      541 aacctttatc agaattgcac cttcgccacg tggcagaaac accgacacaa gagtagtgat
      601 gccaacagaa gaaaaaatga caggttttgt ttttaaaatc catgcaaaca tcgacccaaa
      661 tcatagaagc agaatcgcat ttttgcgcgt atgctctggt aagtttgagc gaaataaata
      721 tttctatcac actcgactaa aaaaggaatt acgttttagt aatccagtaa cctttatggc
      781 atctgataaa aatattgtcg aagatgcata tcctggtgat gtaattggta tgaatgatac
      841 tggaaatttc aagataggag acaccttgac agagggcgaa aacatgatgt ttaaaggtat
      901 tccaagcttt tcaccagaaa tttttaaaga gttggtgaat acagatccaa tgaaaactaa
      961 acaactcgaa aaaggtattc aacaactaac agaagagggc gtggcacaat tatttactgt
     1021 gcatacgaca caagcaaaaa ttattggtac agtaggcgaa ttacagtttg aagtcattca
     1081 atacagatta ttgaacgagt atggtgcatc ttgccagttt aaacctttac agtattataa
     1141 agcttgttgg ttaacggctg aaaatcaaac aaaattaaat gagtttatca gaacaaaatc
     1201 caactatata gcgtttgata aagagaaaca gccagttttt ttggcaagtt ctgaatggat
     1261 tttaagatta acacaacaag attatcctga aattcatttt cacttgactt cagaatttaa
     1321 taaggatgaa gtagttgttt agatgcaata ttgaaatcca ataaattgca ttatttgtat
     1381 tattgaagca aaaaaaatat tactaacatg aacaagcgca ttgatttttt acgttcacaa
     1441 attgacaaag aacagtggca atacgattat cctttgatgg attggctcag agcacgtgtt
     1501 gttgcagtcg atgaaggtta tgtaaaaatg cagtttactg tcgaaaaata tatgcttaat
     1561 ccaattggca ttatgcatgg aggcatcatg gcgactgtct tagatgaagt gatgggtgct
     1621 gctagtttca cattgggccg accaaatgga tttgccactg taaatatgaa tgtggactat
     1681 ctaaatccag caaaattagg agaaaccatt tttgcaattg gcaaaattgt gcgagcaggt
     1741 aaaacaatat tgcatataga agcaagtatt gtaaatgcaa atgataaatt actagcgaaa
     1801 gctacttcca atatgattgt cactgctgtg caattgccta tttaggctac aacttttagc
     1861 acttcctaca cctttctcgg tatttttctt acaagtgaga aatcatctta tttcttgata
     1921 aaagacaaga cgaaagtaat agatgtgata taattttagc aaaacaaata taaaaaaaat
     1981 acggagtggc gaaaccactc cgtgtaagaa ctaaactact aactatttaa tttaatatgt
     2041 gggggagaaa ctaaatctaa ttaattgcca ccagtagggt caacgatgtt gccaccagta
     2101 gggtcaacga tacgtgcact tgctgtggag cttacagtga aattaccacc ggttgggtca
     2161 acgatgttgc caccagtagg gtcaacgatt ctttggttaa ctgttaaatt accaccagta
     2221 gggtcaacga tattgccacc agttggatct acaattttta aaagcgctaa gatgattgtt
     2281 actataattc ccataattgt ttgttttgta atacaaatat ggcaactatt tcttatgtgt
     2341 ttatttcgtt tttaacaata atgtgactgt tcaataattg gtatattatg aatttatgat
     2401 acaaaataca ttgattatca tacagttata tgtaatttct ttgggttttt acttcatttt
     2461 ttgtgacaaa ggtttaaatg ttgggttttg taaataatgt tttctagtag tcgtatttta
     2521 aataatccgg gttgttttgc tctatcaggg actaaataaa tagctaaaat gttaggcatt
     2581 tagtctattt atattaaaat aaatcactac tacagcgcaa agtttgctta taataaagca
     2641 tttgtcaatc tcttgataaa tatatttgtg actttgtttt ttttagtata atttacaaaa
     2701 cttattgcaa ctcgcttgtt tttaagtatt ttgtctattt tttttatgcc tattgtgaca
     2761 ttatggacac cttaaaggaa ttagtaacga ttgttaatag aaaacgtttg tctaaaatag
     2821 atgtttttga taaggcattg cttagccaaa gtgcttccaa tttatattat aagctttatg
     2881 aaggtttaga atctggtaaa atcacagatg acgcaagtgc ggcaagttat atatatggtt
     2941 ctgatgataa agacgcaaaa tatcgcaagc taaaatctag atttaaaaac aaacttcaaa
     3001 aaacactttt attattagat acagaggaag tatttattaa taatcagagt agagcttatt
     3061 atgaatgtct tttaaatagt caaataattg aaatcatcat caaaattact ggtaccacaa
     3121 aattagtgta tgaattagcc aaagaaaatt atagcaaggc agaacaatat aattttaatg
     3181 aaatccttaa aaattattct tattatttat taagctatta tgcaataata ggtgatcaaa
     3241 aatctttttc aaaagaagag caactctatt taaaatattt agatctttct caaaaagaac
     3301 aatatgcaaa gtttttatac tttaaagcat cgataaaatt tgaaaatcaa gcaccaatta
     3361 cggatgcttt gattgaagaa gtaagagtag atgttgaagc attaaataag ttaagattag
     3421 aacttcagaa catagaaata gatttttatt attttttttt ggcactcgac ttttatcaga
     3481 gaagcaatca aattaataaa ttaattgaag taagtaatag tgcagaagat ataatgactc
     3541 taaaaccttc ggcatttacc tatactcgaa aaacgattat ttacatttat aaattagctg
     3601 cattgttatc aaataaacaa tatgatgaag tcaaaaatat cttcaataat attgaacaac
     3661 ttaatgtacc agaaggaaat tataattggt atttattaca agatattcat ttcaaaacct
     3721 acttacaatg taatgatatt gagaatgcaa caaaaattta tgaaactgta gtcaacggaa
     3781 aatcatttaa acgccagaac gaaaaagtgc aggaaaaatg gattatctat catgcgtatt
     3841 tggtgtttat ggataattat attcaccatg gcgattataa atttagtttg gctaaattta
     3901 taaatacggt tccagtcaat tcaagagata aatcaggatt taatttcgcc attcgtatta
     3961 tagaaatatt attcaatgct gcgcgcaatg attttgaact tattttttta aaaatggacg
     4021 ctttgcgtat ctataaatca agatatttaa acgataacac ctataaacgc aatcacattt
     4081 tattaaacct attgctaaaa gcagaaaaat caggctttag ccataaagtt ttagtgaatg
     4141 ctgattggaa agaaattaca gatattagaa aacaaaatgc taatatcgta gccgaatggg
     4201 aaattatgcc ttatgagata ctttgggata tctttgttga gtttactaaa aaagaataaa
     4261 catttctata atacttgcat cattttttgt aattcaaaaa gattgaaata cttttgacaa
     4321 acggatgatt aatacagaac tcttcatagc aaaacgatta gcattaggtg ataaaaattc
     4381 tttctcgaga tttattataa gaattgcagt cgtagctatc gcattgagcg taaccgtaat
     4441 gattgtttcg acatgtatgg tgtctggttt ttctaaagaa attaaagaac gaatttttgg
     4501 cttttgggga caagtgcaca tcacaaattt cgccaataac gaatcgctag aaacgatgcc
     4561 aataaaaaaa gagcaagttt tagtagataa aattagaaat gctaaagatg tcaaaaatat
     4621 tgcacctttt atcaccaaag ctggaatatt aaaaacaaaa acagacatcg aaggtattgc
     4681 acttaaaggc attaataaag aatacgattg gaaaacaatt cagtcctata taaaacaagg
     4741 aaacattata gcttataatg acagcagcgc aagtaaagat attctcattt ctcagtcaac
     4801 tgcaaaacgc ttgcatttgc aagtaggtga taaggtaatt gcctatttta ttcgcaaaga
     4861 attagcggcg cctatcggca ggaaacttca ggtttgtggc atttttcata ctggcctcga
     4921 agaatatgat aagcaatatg cattggtaga tatggaatta cttcgaacac tcaatcaatg
     4981 gcaagccaat gaagtggcag gttatgaaat taggctacat gatatgaata aaatggacgc
     5041 attcaaagat tctgtgtatt ataaatatgt cgatcaaagt gtaaatgcac aaacaatgaa
     5101 agacattaat cgcaatattt ttgaatggct cgatttgcaa aaagtgaatg aatatttaat
     5161 agttgggttt atggttattg ttgccatgct taatatgatt acagcgctaa ttattctaat
     5221 tcttgacaga accaatatga ttggcatttt aaaagcatta ggtgccaaca gcaaaagcat
     5281 caccaatatt tttttataca acgcttctta tattatctta aatggtcttt tttggggaaa
     5341 tttatttgga ataggcttgt gtttattaca gcgatttact ggttttatta aactcaacga
     5401 agaaaattat tatctaagtg ttgcacctgt tgattttaat atcatagcaa ttctacttat
     5461 caatgcaggc actttagtaa tatgcacttt ggtcttgcta ttgcctacac aattaataaa
     5521 taaaatttcg ccgataaaag ccatacgttt cgaataataa attcagtatt tcgagcatca
     5581 catacttttt attatattgc acaaatgttt caaaaactac tgaaatggaa atggatactc
     5641 ttagcattta tttgctatct attacttatg gtattgccag cttcatttgt tgaaaaatat
     5701 tattccaatg ctttctttct ttttgttaga aatttgttgg atagtagcat cgctaaaatt
     5761 ccatttcctg ctatttatat ttttatagta atcgtttttc tactactatt gaaatggttt
     5821 cttcattttt ttaaagaaaa accacagcca ttctttcaac gtatttttag tctcacttca
     5881 agcattgcag cattcatcgt cttcttcttt gtcctttggg gttttaatta tggcagaatt
     5941 ccgatagaag aaaaattaaa catcaatcca gttactatta gcaatattca acttttggaa
     6001 gaaactaaat tcacaataaa gcaactcgaa aacttaagaa aaactctgaa acgcgatact
     6061 ggctctctgc ctcaaataat ttttataaat aatatcgaaa ccataagcag agatgcattg
     6121 aataaaacat tacaaaattt ttcctatcct tatagcagta aaattagagg acggtttgta
     6181 gtagaagata tgttcctagt gtttagcatc ggcggacaat acttacctta tgttggagag
     6241 gccaatgttg atgatgctgt atttttttcc aaaaaaccat tctacctcat tcacgaatta
     6301 gcacatggta atggttttac cgaagaagct acttgcaatt ttcttgccta tgtgagctgt
     6361 aaacaatctg gaaatgtggc tcttgaatat agcggtgaac taaattactt attatattta
     6421 ttaagcgaat taaaatatag agatagtgtt gcctttgatt cgctaatgct ttctttacca
     6481 cacaatatcg caaaggattt ggcagaaata aaacaacata ttgccgaaca tacttttaaa
     6541 acagctttct ttggcgactt agtcaataat ttatacctca aaatattagg cattcctgac
     6601 ggcgttaaaa actatgacaa aatgcttttg ctagtctatg cttggaaaca tcaatatcct
     6661 aaatagcttg ttttatttgc cttaatcgaa tttatttttt gttcaatttg ctggattgct
     6721 aaagcataat atagaagtca atatattgtt aagttctgtt ttattaatct aggtcaatat
     6781 tcctaaaaca atttattcct aactttgtta actaatctaa ctttgttaac aaaagtatgc
     6841 ctaataaaga acgcagacgt aaagaaaaag aagaattaca acagaaaata ttagctgttg
     6901 caaaagatat tgctgcatct gatggttggc aaaatgttac tattcgcaaa atttgtgatg
     6961 aaatacatta taccgcacct gttatttatc aatacttcga aaacaaagag gctattttac
     7021 agtctttacg aactgatggt tttattcagg tttttaactt atttgagaaa gtaaatgcca
     7081 aacatcaaac tgcagataaa aggttattgg aatatgcgat tacttggtgg aattttgcta
     7141 ttcagcatcc tgaaatttat caagtcatgt ttaatctgca tggtgccgtt tgtgttcata
     7201 atgatagcaa tataaaagta cacaatacgg taatggatta ttatcatcct gcttttaaac
     7261 aaattaatca aaaggcaaaa cgctctgaaa tatatagcct tgaactgaca gatagcttta
     7321 ttgcaattat acatggcttt atttcaatga acatggcaaa caaaattaaa tctggaaata
     7381 ataaggcaac tcaagtattt aaaaatagca taatcagatt tatacactca cttaaagaat
     7441 aatatttata caatgaaaat aggaaaaatc atcttctgga catcgattgt tttactcata
     7501 gcaggattag cttacataaa acatactaac aataaaaaag cagcagcaaa agcaacgcca
     7561 ttcggcaagt ttggcccttc aactttaaga gcgacaggct atgttgctga atatactaaa
     7621 ttgtcaaaca tgctaagtgc aaacggaaac attttagcac aagacgaaat acaattacag
     7681 ccagaagtat ctggtcgtgt tacctatctt aatattaacg aaggtagtgt tgtagctaaa
     7741 ggaactttgt tgatgaaaat taatgatgca gatttaaaag cacaagtggc taagttgaat
     7801 acacaacaaa aaattgcaca aacaaatttt gatagagtag cagcactact tgctattaaa
     7861 ggtatttctc aagcagaata cgatgcagca gaaaacacat taaacaacat caaagcagat
     7921 attcaattac tacaagtgca aattgataaa acagaattgc gtgcaccatt tacaggtaaa
     7981 ttaggcttgc gcaaaataag tttgggcgca tatgtttcac caaatactat aattgcatca
     8041 ttacaaaatg taagtcaact taaaatagat gtttctgttc ccgaaaaata tgcaggcatt
     8101 atacacgtcg gcgataatat gcagtgtaaa gtagctggtt tcgacgaacc atttacagca
     8161 aaagtaattg cagtagaacc attcatagat gatattactc gaaaccttac gatacgcgca
     8221 attatacaac aacacaatgc aaaattaatt ccaggtgcgt atgcaaaagt agaattaaaa
     8281 ctaaaagatt caccaaactc aattatgatt ccgagtagtg ccattattcc agatgataga
     8341 tttaccaaag tagtagtgac agacagcagt aaagcaaaat ttgtaaatgt agaaattggt
     8401 gtgcgcacag agagtgaagt gcaaattatt agcggaatta atattggcga tacggtcttg
     8461 acatctggct tgttgcaagt aaaaccaggc atgtccgtac agattacaaa agctacaaca
     8521 cgttctacta tcaaataaga aagtatatgc agcattcatt tttaaattat taattcaaca
     8581 tcatgggttt accttcatta agtttacgac gtcctgtttt cgcaatagtt atcaatatta
     8641 tcattattct tttcggtgtt attggttata catttttagg tgtgcgcgaa tatccatctt
     8701 tggatccacc tgttattaat gtacgcacga gctacacagg cgcaaatgcc gatatcatcg
     8761 aatcacaaat tactgagccg ctagaaaatg cgattaatgg tgtgcaagga atacgcacta
     8821 tttcttctgc aagcaatcaa ggttcttcca atattacagt ggaattcgac ttaggcgctg
     8881 acctagaaac tgcggctaat gatgtgcgcg ataaagtgtc gcaagctatt cgtttattac
     8941 cgcaagatat tgatgcatct ccagtggttt ctaaagcaga tgccaattca gatgctatca
     9001 tttccatgac tatccaaagc gattcgaaga atgtattaga gttaacagat tatgccgtaa
     9061 atgtattgca agaaagagtg caaactattc ctggtgtgag ctcaattcaa atttggggtc
     9121 aaaaaaaata tgcaatgcgt ttatggtttg acccacaaaa attagcagcc tatcaactta
     9181 ctgcgattga tgtacaaaat gcattgaaca aaaccaatgt ggagctgcca tcaggtaaaa
     9241 ttgctggaca aaatactgag cttaccgtaa acacgcttgg aagaataaat acacctgatg
     9301 aatttaataa catgattatt aaagatgtaa aaggtaaagc agtaagacta aaagatattg
     9361 gctatgcaac cttaggcacc gaaaattttg aaacttcttt gaagaacaat gctgtcggca
     9421 tggtggcgct cgccttagtg cctcagcctg gcgcaaacta tgttgaaatt tctgatgaat
     9481 tttataaacg attagaaaaa ataaaaaaag aaattccgag tgatataaaa ttagatattg
     9541 ctttagataa tactgtattc gtaaaaaatt ctattctaga agtaaaagaa acgcttttca
     9601 ttgctattgt tttagtggta attattatct atttattttt tcgcgattgg gtaattgcca
     9661 ttcgtccatt aatagatatt cctgtatcat tgattggtgc atttttcatt atgtatattt
     9721 tcggatttac cataaatgtg ttgaccttgt tggctatcgt cttagcaact ggcttggtcg
     9781 ttgatgatgg aattgttgtc actgaaaata tattcaaaaa gctagaggca ggaatgccta
     9841 aaatgcaagc ggcacgggaa ggctctgaag agatattttt tgccgttatt tctacttcta
     9901 tcacattggc cattgtgttt ttgccgattg tttttatgca aggttttgtt ggtagtttat
     9961 ttaaagaatt tggtattgta gtagcaggag ctgtactcat ttccgccttt gtgtcgctca
    10021 cgctgacgcc tgtactcaat gttattttag gaaaaaaagt gcataaaaat tcttggtttt
    10081 atgacaagac agaacctttc tttaaaggca tggaagatgg ctatagagat atgctgaatt
    10141 cgtttatgaa aaaacgattg atagcaattc caattgttgt gctgtgttta gcattgatat
    10201 attttattgg caaaaatttg caatcagaat tagcgccaat ggaagataga ggcgcattcc
    10261 gtacaatgat gtcggctccg gaaggaactt cttatactgc tatgcaagat tatgtggaca
    10321 aagtttctga gtttgttatc gattcagtac cagaacaaag attaatgttg agtgtgacat
    10381 caccaggttt tacaggttca ggtgcagcca attcagcttt tatgcgtata ggtttaaaag
    10441 ataaaagtga acgcacacgc tcacaagatg atatagcaca atatttacaa cgcaattttt
    10501 ctaagtttaa tttagggaaa gcattcgtaa ttcaagatca aacaatttct gtaggctccg
    10561 ctcgttttgg tcagcctgtt caatttgtga ttcagaattt tgattttgaa aaattacaaa
    10621 atacaattcc taagtttatg gaagaagtat ctaaaagtcc tgtgtttatt ggtgctgacg
    10681 tgaatttgaa atttaacaaa ccagaagtaa atgtaattat tgataaagat aaagcaacgg
    10741 ctttaggcgt ttctgtacaa gacatcgcac aaactttaca gttttctatg agtggccgac
    10801 gatttggtta tttcttaaaa gatggaaaac aatatcaaat tattggacaa gcacaatatg
    10861 aagaaagaaa caaaccagaa gatttaaaaa atatttatgt gcgaaataat gcaggtgctt
    10921 taatttcttt ggataatcta attagaattg aagaattggc gaagccaccg caattatatc
    10981 attatgatag atacaaatca gcaacagttt catcaggctt agcacctggc aaaacagtag
    11041 gcgatggaat agcagaaatg gaacgaatag caaaacaagt gttagatgaa tcatttacga
    11101 cttccttgtc tggaacatct cgcgactttg ccgaaagttc atcgaatacc atgttcgcat
    11161 taatccttgc attagtgttg atttatttag tattagctgc acagtttgaa agttttatcg
    11221 atccatttat tatcatgatg acagtgccat tggcattagc tggcgcatta ttatcacttt
    11281 ggatatttgg tcaaacactc aatattttca gccagattgg tatgattatg ttgattggtt
    11341 tagttacaaa aaatggcatt ttgattgtag aatttgctaa tcagagcaga gcaaaaggaa
    11401 tgggaaaatc agaggctgtt gcttatgccg cagtaatgcg cttgcgccca atattaatga
    11461 caagtttggc aatggcgctc ggcgctgtgc ctattgctat tgcatttggc gcaggttcaa
    11521 caagtagagt ttctttaggt attgttatta ttggtggaat tatgttctcc ttaattttaa
    11581 ccttattcgt cgtgcctgcc atctattctt tattatctag aacaacagta aaaacagtag
    11641 aataatgaaa ttatatagaa tattattagt gagcatttgc ttacatagtt atgtgtcatt
    11701 acaagcacaa acattgctca caccagaaca agctgtaact acggctttgc aaaaaaatta
    11761 tgatattgtg ttggcgaaaa gcgaagctga tattgcaaaa attaataata ataaagccac
    11821 tgctggaatg ttgccaatta taaatgtaac tgcaggaaat gtgtttaatt tgaacaatat
    11881 caatcagaaa tttacaacag ggcaggaagt gaaaaagaat tgggtgcctg tcaattcttt
    11941 cactacagga ttaaatatga attggacaat ttttgatggc atgcgtatgt ttgccgcaaa
    12001 agatagatta gcagcattgc aatcattaag cgaaatgcaa ttgaatgacc agattcaaaa
    12061 tacaatagca caagttttat ctgtttacta tgaaattgtg agacaaaaac aattgataaa
    12121 agccttgaat gaatctttga aaataacaga agagcgcgtt tttctttcca ataaaaaatt
    12181 agaagttggc tatgcagata aaacgccatt gttgcaagca aaagttgatt tatcttctca
    12241 gaaaataaat atcttgaaac aagaaacaca attgcaacaa tcaaaagttc aattaaatca
    12301 gctcttagga cgaactgcta atgatttatt tgatgtgata gatacaattg aaattaaatc
    12361 ggctttgaat gccttaccaa cattagatgc gattcaccaa aataatttat taattaaagg
    12421 tgcaaataaa aatattgaaa ttgcgaagtt gcaacacaaa gaaataaaag caaaacgaat
    12481 gcctcaaatt aactttaata caagctataa ctttatgcaa aataatagta aagctggttt
    12541 acaattattc aatcgtagct atggaccaca gcttggtgtg caagcaagta ttcctatttt
    12601 taatggtggt atggtgaaaa aagaattaca agtttctgct gtaaatatcg caatgaagca
    12661 aattcaagtt gaccaaataa aaaatagttt agatgctcaa ttgcaatcag catataatga
    12721 ttttgattac gctactaaag tgttagattt aaacgaggac aatgtgaaaa ctgctgctga
    12781 aaatgtgcaa gtcacattgg aaagatttcg tttaagccaa tcgacatctt tagaaattaa
    12841 acaagcacaa agtagttatg aagatgcttt atataatgtg gttttggctc gatataatgc
    12901 aaaaattgct gaaattgcat tgcagaaatt aaacaacgca cttccaaaat agtatctatt
    12961 ttgtttttat atgcaaggct ttcaataatt ttttggcatc actttcttcg tctatttttt
    13021 taatgaattg cttgttttcg tcgaataaaa ttaaacaggg aaaactcgaa tattgtattt
    13081 gtttggttaa tgtgtaatca atctcttggc caataaatac agttgtgtaa gaagacaaac
    13141 cataatcaaa ataaaattgt cgaatggatg tcaaactctc gaaagaactt aatataaatt
    13201 gagtattcgt aaatttaaaa atattgtcga gtatgctttt gatggtgcgc tggcaatgat
    13261 cgcatgttgg actaaaattg ataaataccg tttttttatt atcctctatt tcatcgtttg
    13321 tcaatatgct actgctgtct atttttaata attgaattat tggaaatttt atctcatttt
    13381 cttttgcttt caaagagaga aaatacagta agctaattaa tgttagaatt gaatttttat
    13441 aaatcatctt ctttcatctt gttgtttgag tatgtttata caaaaataat tccattcttc
    13501 agctttttat aatttccttc tactaattta ttagttcaat attctaatcg tttctttatt
    13561 aggcaaattt agacatcgca aaaaaaagta atatattaat tttcactact gtccgataaa
    13621 gataatgaat aaaaaatcag gtattgattt tcaaatt
//
LOCUS       DAOQQV010000069        12997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1642_length_12997_cov_8.499305, whole genome shotgun sequence.
ACCESSION   DAOQQV010000069 DAOQQV010000000
VERSION     DAOQQV010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1642_length_12997_cov_8.499305"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..715)
                     /locus_tag="PLL59_08080"
     CDS             complement(167..715)
                     /locus_tag="PLL59_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMY23805.1"
                     /translation="MDKDDIIAIIAYLRTLPSIENTTKESKADFPVNLILNTMPKDAT
                     FSERPSKDNFVRYGEYMTNAASCIECHTNAVRGEKIKGMEFGGGREFTFPGNKKIVSS
                     NITPDKETGIGYWTEDMFVAKFKLYADKSKLQDYKSPKDFQTIMPWSQYAGMDTVDLK
                     AIYAYLQSIKPIKNKVISNPEN"
     gene            complement(739..1134)
                     /locus_tag="PLL59_08085"
     CDS             complement(739..1134)
                     /locus_tag="PLL59_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23806.1"
                     /translation="MKKWILRIMAILLVLIISGIAYLKFALPNVKAPENLKITATPEM
                     IKRGEYLANYVTMCVDCHSTRDWSKFSGPLIESTIGKGGEAFLHEYGFPGNFYSKNLT
                     PFNLKTGLMVSYCEPSAVVLIKMVMHFFQ"
     gene            1389..3179
                     /locus_tag="PLL59_08090"
     CDS             1389..3179
                     /locus_tag="PLL59_08090"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HMY23807.1"
                     /translation="MKQEHQANYAAPDTRYFKTQIDFDKAVSKDFINAANQVTLKKQK
                     FLVCLSHGQSPAGAYQQIVHFYQKIKKPENVFYTFLNSPLKSQENLENVFTATVFLEK
                     LLQKNFITPNQILADFDNMQDIDAYATTLNDTLEGFLQANNKTGFDYVFMASNPKGSI
                     GAIERNSSAFQSDKIIDVVNISGEKKITVTPQFLLQTKRIAFLATKADKRRPLAWLYS
                     TTGKEHESPSFIRYIENVEERVAVFIDDTALTWPQIEIKRETPYGVSKIRVDVTYPYK
                     ENAKVKLPVIVMVHGFLGLNSYDGLLTTIPTTKYIAAAMHYGTIPTDLPLDDYSKHVA
                     QNIDAVIGFFGAKGHPVYLFDHSMGNIYFLMMDKNPNDYKNVKKYLRGRIGANPFFGE
                     EAKHALLGFLDNVIIPSMSFTENPIEKSLVLSFRRVAPLDSKSGVRKRGINLAKTLVG
                     KEESNTNAIWDLIKSRIVFLMSNMDSLPHLNRIPIEKALNKVPAKVFGIQVYSALKES
                     IQFDKQTSLVNSKIHNIPILILKSERDGVAKFVPRIYENEYDVEIVDVTNYADKDLFR
                     EHLYHMVNPQQTVAIIDKFISKIENDHKSI"
     gene            3323..4405
                     /locus_tag="PLL59_08095"
     CDS             3323..4405
                     /locus_tag="PLL59_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HMY23808.1"
                     /translation="MKFTEQQIIHALSQVEDPDFKKDLVSLKMIKDLSFTDDLIKFTI
                     ELTTPACPLKEQMENDCRNAIAQLVSNDVKVEIDFSSRVTTLRDVKSDILPSVKNIIT
                     VASGKGGVGKSTVSVNLALALLRTGAKVGLIDADIYGPSIPTMLGLQNARPSIQKVDD
                     KNKIVPVDYLGMQVMSIGFLIQDKQPVAWRGPMATSALRQIITDVLWEELDYLILDLP
                     PGTGDIHLTLAQLVPVTGAVVVTTPQEVAMADARKAIAFYQLESIHVPVLGVVENMSF
                     FTPLELPDKKYYIFGKGGGRLLAQQFEVPFLGELPIEQDIREGGDLGKPIALQTATIA
                     AAAFEQLAQTVARQIAVQNANAEVMS"
     gene            4416..4664
                     /locus_tag="PLL59_08100"
     CDS             4416..4664
                     /locus_tag="PLL59_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HMY23809.1"
                     /translation="MTEQIHNQDLHTKVEMALNNIRPFLQSDGGNIELIEVTNDFIAK
                     VKLVGACESCKMSASTMKAGVEATVKNFVPEIVAVIAV"
     gene            4697..5509
                     /gene="pyrF"
                     /locus_tag="PLL59_08105"
     CDS             4697..5509
                     /gene="pyrF"
                     /locus_tag="PLL59_08105"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMY23810.1"
                     /translation="MTRKQLIQQIKKKKSYLCVGLDTDITKIPPHLLKYKNPILEFNK
                     QIIEATHDVCVAYKPNIAFYEMYGAKGWETLEKTVQHIPNHIFKIADAKRGDIGNTSN
                     MYAKAFLENMPFDAITIAPYMGEDSIKPFLEIKNKWVIVLGLTSNKGAQDFQFLKSNK
                     KPLYQNVMEKCMQYGTADNMMFVIGATKALYFKDIRKFCPDHFFLVPGVGAQGGDLQA
                     LSQFGMNKDCGLLVNATRSIIYASNGKDFAERARVEAIRMQQEMKLLMHAIV"
     gene            5624..5956
                     /locus_tag="PLL59_08110"
     CDS             5624..5956
                     /locus_tag="PLL59_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMY23811.1"
                     /translation="MKKVKYLMAISLLAVVMISTSCKKKCVEPAGVTSSFTYEITGYV
                     EGGATIEFTNTSTSATSYLWDFGNGQTSTETNPTRVIGVSGDAEVKLKSTNGKSTATS
                     IMKIEFPF"
     gene            5968..6300
                     /locus_tag="PLL59_08115"
     CDS             5968..6300
                     /locus_tag="PLL59_08115"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMY23812.1"
                     /translation="MKKIFGILFIALAIFATSCKKKCLDPSADLVANFDLPIEPYTDN
                     ELTFNNTSIGAVSYEWDFGDGTPKSHSLHPKHTYETDGWYSVKLTASDGNISATSEKG
                     FYVSSEGD"
     gene            6430..7560
                     /gene="moeB"
                     /locus_tag="PLL59_08120"
     CDS             6430..7560
                     /gene="moeB"
                     /locus_tag="PLL59_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HMY23813.1"
                     /translation="MQEIQLSPEEIARYSRHLLVEEFGMEGQLRLKQAKVLVVGAGGL
                     GCPLLLYLAAAGVGTIGIIDFDTVEESNLQRQILYTIDDIGKPKVDVAKKKLLAINPY
                     IHIITYQQQLSAKNALEIFSSFDIVADGTDNFVTRYLVNDACVFSDIPNVYASILQFE
                     GQVSVFNYKEGPNYRDIFPVPPAPNTIPNCAEAGVLGVLPGIIGSMQANEVIKVISGI
                     GTPLSGRLFIFDALHFSSKIFTISKDKNNPLTGDNPTIKALTDVDLFCGATSSITNEI
                     SVQQLKEMFDTKEDIQLIDVREHYEYNQVNLGGICIPLHQLKQSMDLISKEKKVIVHC
                     KTGMRSAKAVQLLLDNGYRNVYNLNGGIIVYAKEIDNQLPLY"
     gene            complement(7621..8322)
                     /gene="radC"
                     /locus_tag="PLL59_08125"
     CDS             complement(7621..8322)
                     /gene="radC"
                     /locus_tag="PLL59_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HMY23814.1"
                     /translation="MYTPAKLSIKSWAEEDRPREKLLLKGKHSLSDAELLAIIIGAGN
                     TENSAVELSRQILAAVHNDLFELGRKPLDFLMQFKGIGEAKAISIAAALELGRRRTIA
                     ESKPQDGIISCKAAFEIISPILSDLPYEEFWIICLKRNNRFISKHKISTGGVSGTVVD
                     MKIVFNIAIKELASGIILCHNHPSGNLNPSDQDIRLTKQAVEAGKLFDITIVDHIIIA
                     HNNFYSFTENNNMYF"
     gene            complement(8386..8643)
                     /gene="rpsT"
                     /locus_tag="PLL59_08130"
     CDS             complement(8386..8643)
                     /gene="rpsT"
                     /locus_tag="PLL59_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HMY23815.1"
                     /translation="MAHHKSAKKRIRQTVKRRLENRYHGKTTRNALRKYLGLSDKNEA
                     EKSMSAVVAMVDKLAKRNQIHKNKASHLKSAVMRHLNALKK"
     gene            8824..11586
                     /locus_tag="PLL59_08135"
     CDS             8824..11586
                     /locus_tag="PLL59_08135"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HMY23816.1"
                     /translation="MEDLSILSNAHPSYIDALYKDYLKDQNSVDAEWKKFFAGFDLAY
                     KYGENGALTSENANADTQATNANVVSPKEFKVLKLISGYRNKGHLVAVTNPLRPRKDR
                     HADLDLHFFGLEESDLDVEFYAGKEIGIGRATLRTIIERLQKIYCRTIGWEYNYILDR
                     DERAWMREQIEHGYIAYEHPIEKKKRILSKLNDGEVLERFLGTKYIGQKRFSLEGGES
                     TIPALDAMINIAANHGVEEIVIGMAHRGRLNVLTNIVGKTYEQVFSEFEGVAPNDFSS
                     GTGDVKYHLGYSSQYDTMDGKEVYINLLPNPSHLEAVNPVVQGYCRAKADAIYESDYD
                     KILPITIHGDSAVAGQGIVYEVLQMQSLKGYTVGGTIHFVINNQIGFTTDFDDARTSN
                     YCTSIASTIQAPVFHVNGDDVECVTYVAELAAEYRQRFNKDVFVDMVCYRKWGHNEGD
                     DPSYTQPQMYKIIKDHVGVRDLYQKKLYDWGVAEAEFAKKMEEEFWQELQARLNDYKQ
                     QEKKYKPQSTELAWKALRRSTNADFEQSPETKISKDNLVKIVKALAKTPEGFKPLKKI
                     QQYLDQRRVLMRENNSVDWAAGELLAYGSILLDGKDVRLSGEDVKRGTFSHRHACVYD
                     EVTGQEYNRLNFIEEDGKQGTFHIYNSHLSEYGILGFEFGYSLPSPAPLTIWEAQFGD
                     FDNGAQIIIDQFIASCETKWNRQSGLVLLLPHGYEGAGPEHSSARLERFLQLCGENNM
                     IVTNITSPANFFHAIRRQLTWSFRKPMINMSPKSLLRHPKCVDPISKIYEGKFQEVID
                     DENTLDDVSVKKVLFCTGKIYYDLLAKKEADNRTDVAIVRVEQLYPVADKQLDAIIKK
                     YSKATYHWVQEEPINMGAYSFLLLNYSKAKYLQCIARPQAASPATGFSKLHEKEQKAL
                     VELAFG"
     gene            11663..11953
                     /locus_tag="PLL59_08140"
     CDS             11663..11953
                     /locus_tag="PLL59_08140"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMY23817.1"
                     /translation="MQPKTIQDIQNYFNEHDLFNKYGLTKIGVFGSFARGEKFNDIDL
                     YVEDDIELGKAAELKVLLENDYNLQIDLVLKAKENKLIMFYAKKDMKYIYAN"
     gene            11943..12389
                     /locus_tag="PLL59_08145"
     CDS             11943..12389
                     /locus_tag="PLL59_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMY23818.1"
                     /translation="MPIREDYIIVLSILQCVGKTVHFTNKFNTADIFFEHNNQLNYLA
                     SLTLLANIGEHANKLSENFKKEHSKINWKQIKGLRNFIVHNYEGVDYIILFNTIKNDL
                     PKLKLELENYLKESILSNCIDKEIFVELALDNPFYEFINFENIIEQ"
     gene            12386..>12997
                     /locus_tag="PLL59_08150"
     CDS             12386..>12997
                     /locus_tag="PLL59_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HMY23819.1"
                     /translation="MSKIEIKVPSIGESVTEVTLSSWLVEDGDYVKLDQALAEFESDK
                     ATFEMPSEATGVITRVAKEGDDVKIGGLVAYIDTDAAAPEKSTGSSQESVEKTEEIKV
                     EDKVEENLKSEISNLQTNYATGTPSPAAKKILDEKGIETTEVKGTGIDGRITKEDAEK
                     AQKSEKVLVKSEENETEKKTNIQQPITSNFSRSERIEKMTRMRK"
BASE COUNT         4500 a         2083 c         2373 g         4041 t
ORIGIN      
        1 tggcaactgg accacatgtt tgttatcgct tttggaaata cggcgcacaa gtcgatccgc
       61 tgaaggaaaa actaaatgta gtaaagcaaa taccaaaaaa tgacatgtcc ttgtatttgc
      121 agtttttttc taccgcaaaa aatattttgg aaaaaattcc aatggtttaa ttttctggat
      181 tagaaatgac tttgtttttt attggtttta tgctttgtaa ataggcataa attgctttta
      241 aatcaacagt gtccatgcct gcatattgtg accaaggcat tatggtttga aaatcttttg
      301 gcgacttgta gtcttgcaat tttgatttgt ctgcatacaa tttgaattta gcgacaaaca
      361 tatcttccgt ccaatagcca atgcccgttt ctttgtcggg cgtaatattc gatgatacaa
      421 ttttcttatt gccaggaaaa gtaaattctc tgccgccacc aaattccatt ccttttattt
      481 tttcgcctcg tacagcattc gtatgacatt ctatacaaga tgcagcgttt gtcatgtatt
      541 cgccataacg tacaaaatta tctttcgaag gccgttccga aaatgtagca tcttttggca
      601 ttgtatttaa aattaaattc acaggaaaat ctgctttcga ttctttagtt gtattttcta
      661 tcgaaggtaa agtgcgcaaa tatgcaataa tagcaatgat atcgtccttg tccatcttgc
      721 tataatttgg atagggcatt actggaaaaa atgcattacc atctttatca acaccacagc
      781 tgatggctcg caataactca ccatcagtcc agtttttaaa ttaaaaggtg ttaggttttt
      841 agaataaaaa ttgccaggaa aaccatactc atgcaagaat gcctctccac cttttccgat
      901 ggtgctctcg atgagtggtc cagaaaattt agaccagtct cttgtcgaat gacaatcaac
      961 acacatcgta acataattag ctaaatattc accacgctta atcatttccg gtgttgccgt
     1021 aatttttaaa ttttctggtg ctttaacatt tggcagtgca aattttaaat aggctattcc
     1081 acttattatc agtacaagca gaatagccat gatacgaagt atccattttt tcatagttag
     1141 cggttgttga atttaaattt atcttatttg tatcaatttg tcaaagcaaa tctcattatt
     1201 ttttcacttt tttatattaa atcttacatt tatgaaataa agcaagtaaa tactttgtat
     1261 ttttttgtct ttaatgaaaa ggaatattct ttagctatgt aattaaattt ttaaaaattg
     1321 atatgcatca ataataattc gaatgcataa tcactttatt tttctatttt tagacctttc
     1381 agaaaaatat gaaacaagag catcaggcaa attatgcagc gccagacacc agatatttca
     1441 aaacgcaaat agactttgac aaggcagtaa gcaaagattt tattaatgca gcgaatcaag
     1501 taacactaaa aaaacaaaag tttttagtgt gtctttctca tggacaatcg cctgcaggcg
     1561 cctatcaaca aatagtacat ttctatcaaa aaataaaaaa accagaaaat gtattttata
     1621 cttttttaaa ctctccatta aagagtcaag aaaatttaga gaatgtattt actgctactg
     1681 tttttttaga gaaattactt caaaagaatt tcattacgcc taatcaaatt ttagcagatt
     1741 ttgataatat gcaagatatt gatgcttatg ctacaacgct gaatgatact ttggagggat
     1801 ttttacaggc gaataataaa acaggatttg attatgtgtt tatggcatcg aatcctaaag
     1861 gtagcattgg cgctatagag cgaaattcct ctgcttttca atcggataag ataatagatg
     1921 tcgtcaatat ttcaggcgaa aaaaagataa cggttacacc acagttttta ttgcaaacga
     1981 aacgcattgc atttcttgcc actaaagcag ataagagacg tcctttggca tggttgtata
     2041 gcactacggg aaaagagcac gaaagcccaa gttttattcg ttatatcgaa aatgttgaag
     2101 aacgtgttgc tgtatttatt gatgatacag ctttgacgtg gccacaaatt gaaataaaac
     2161 gcgaaacacc ttatggtgtt agtaagatac gtgtagatgt aacttatcct tataaagaga
     2221 atgcaaaagt aaaattacca gtcatcgtta tggtgcatgg ttttttaggc ttaaattctt
     2281 atgatggttt attaacgacg attcctacta caaaatatat tgcagcagcg atgcactatg
     2341 gcactattcc gacagatttg cctttagatg attattcaaa acacgttgca caaaatattg
     2401 atgctgtaat tggatttttt ggtgcaaaag gtcaccctgt ttatctcttt gatcattcta
     2461 tgggtaatat ctatttcttg atgatggata aaaatccaaa tgattataaa aatgtaaaaa
     2521 aatatttgag aggcagaatc ggtgctaatc cattttttgg tgaagaagcc aaacatgcct
     2581 tgcttggttt tttagataat gtcataatac cttcgatgtc atttacagaa aatcctattg
     2641 agaaatcatt agtattatca tttagaagag tggcgccatt agactcaaaa tcaggagtgc
     2701 gcaagcgtgg catcaactta gccaaaacac tagtaggcaa agaagagtca aacactaatg
     2761 cgatttggga tttgattaaa agcagaatag tatttttgat gtcgaatatg gattcattgc
     2821 cacatctcaa tcgaattcct attgaaaaag ctttgaataa agtgccggca aaagtatttg
     2881 gcattcaagt gtattctgca ttaaaggaat ccattcagtt tgacaaacaa acgagcttgg
     2941 tcaattccaa aattcataat attccaatat tgatattaaa aagcgaaaga gatggcgtcg
     3001 caaagtttgt tcctcgtatt tatgaaaatg aatatgatgt cgaaattgtt gatgttacaa
     3061 actatgctga caaagatttg tttagagagc atttatatca catggtaaat ccacaacaaa
     3121 cagtagccat catcgataaa tttatatcaa aaatagaaaa cgatcacaaa tctatttagc
     3181 tatattattt acaaacacaa tagagaaata cttaaaaaat cctcgattgt aatgatttga
     3241 ccttaggctt tatctaatct ttacataaat acaacttctt aaatactgct attctttgta
     3301 tttttgtatt aatttttttt agatgaaatt cactgaacaa caaatcatac atgccttatc
     3361 gcaagtagaa gatccagatt tcaaaaaaga cttggtttct ctgaaaatga taaaagattt
     3421 atcatttaca gatgacttaa tcaaatttac aatagaattg actacgccag cttgtccact
     3481 caaagaacaa atggaaaatg attgcagaaa tgccattgca cagctcgtat ctaatgatgt
     3541 aaaagtagaa atcgacttca gctccagagt aacgacattg cgcgatgtaa aatcggatat
     3601 cttaccttct gtaaaaaata tcataacagt tgcatcaggt aaaggtggcg taggcaaatc
     3661 tacagtgtct gtaaatttgg cattagcact attgcgtact ggtgcaaaag taggtttaat
     3721 cgatgccgat atatatgggc catcaattcc aacaatgtta ggtttgcaaa atgcacgacc
     3781 ttctattcaa aaagtagatg acaaaaacaa gattgtacct gtcgactatt taggaatgca
     3841 agttatgtct attggttttt taattcaaga taagcaacca gtagcgtggc gcggaccaat
     3901 ggcaacatca gcattgcgac aaataattac agacgtgctt tgggaagaat tagattattt
     3961 aatcttggat ttgccgcctg gtactggtga tattcacttg actttagcgc aattagtgcc
     4021 agttacaggc gcagtagtgg tgaccacacc acaagaagta gccatggctg atgcacgtaa
     4081 agcaattgca ttttatcagt tagagtctat tcacgtgccc gtgttaggcg tggtagaaaa
     4141 tatgtctttc tttacgccat tagaactgcc agataagaag tattatatat tcggcaaagg
     4201 tggtggtcgc ttgttggcac aacaatttga agtgccattc ttaggcgaat tgcccatcga
     4261 gcaagatatt cgcgaaggtg gtgatttagg aaaaccaatc gcattacaaa ctgcaacaat
     4321 tgccgcagca gcatttgaac aactggcaca aacagtagca agacaaatag ctgtgcaaaa
     4381 tgcaaatgca gaagtaatgt cgtaacttta tagcaatgac agagcaaata cataatcaag
     4441 atttacacac caaagtagaa atggctttaa ataacattcg accatttcta caatctgatg
     4501 gtggcaatat cgaattgata gaagtgacaa atgattttat tgcaaaagta aaattagtag
     4561 gcgcatgcga atcctgtaaa atgagcgctt ctacaatgaa agcaggcgta gaagcaaccg
     4621 taaaaaactt tgttccagaa attgttgccg taattgcagt gtagattttt ataaaaaaat
     4681 agtactatca ttatacatga ctcgtaagca acttattcaa caaatcaaga aaaagaaatc
     4741 atacttgtgc gttggtttag atacagatat tacaaagatt ccaccgcatt tattaaaata
     4801 taaaaatccg attttagagt ttaataagca aatcatcgaa gctacgcacg atgtatgtgt
     4861 ggcatataaa cctaatattg ctttttatga aatgtatggc gcaaaaggct gggaaacatt
     4921 ggaaaaaaca gtacagcata tacctaatca catttttaaa atagcagatg ccaagcgagg
     4981 cgatatcggt aatacatcaa atatgtacgc aaaagcattt ttggaaaata tgccatttga
     5041 cgctattacg attgctccgt acatgggcga agattccata aaaccatttt tagagataaa
     5101 aaataaatgg gtaatcgttt taggcttaac atcaaataaa ggcgctcaag attttcaatt
     5161 tctaaaaagc aataaaaaac cattatacca aaatgtaatg gaaaagtgta tgcaatatgg
     5221 cactgcagac aatatgatgt ttgtcattgg cgcaacaaaa gcattatatt ttaaagacat
     5281 tcgcaagttt tgccctgatc atttttttct agtgccaggc gttggcgcac aaggcggcga
     5341 tttgcaagca ctttctcaat ttggcatgaa taaagactgc ggtcttttgg tgaacgcaac
     5401 acgcagcatc atttatgctt ctaatggaaa agattttgct gagagagcaa gagtagaagc
     5461 cattcgtatg caacaagaaa tgaagctact catgcatgca attgtttaat tgaagtacca
     5521 tacatacttt ttatctctct tcgttttatt aataatattt ttttggtgtt gcttttaaaa
     5581 aaaaattata ctatatttat ttttattaac caaaaataaa attatgaaaa aagtcaagta
     5641 tcttatggct atttcattac ttgctgtagt aatgattagc acctcgtgta aaaagaaatg
     5701 tgtggagcct gcgggcgtca cttcttcctt tacgtatgaa atcactggtt atgtcgaagg
     5761 aggcgcaact attgagttta ccaatacttc aacaagtgcc acttcttatc tatgggattt
     5821 tggcaatggc caaacctcca cagaaacaaa ccctacgcgt gttataggag ttagtggaga
     5881 tgcagaagta aaattgaaat ctaccaatgg aaaatccacc gcaacatcta ttatgaaaat
     5941 tgaattccca ttttaaaaat taatcctatg aaaaaaatat ttggaatact atttattgct
     6001 ttagcaatat tcgccacttc ttgcaagaaa aaatgtttgg atccttctgc tgatttagta
     6061 gctaattttg acttgcctat tgagccttat actgataatg agttgacttt taataacacc
     6121 tcaataggtg cagtttccta tgaatgggat tttggtgatg gcacgccaaa gtctcactca
     6181 ttacatccaa aacataccta tgaaactgat ggttggtaca gcgtaaaact aacagcatca
     6241 gatggaaata ttagcgcaac atctgaaaaa ggtttttatg tttcttctga aggagattaa
     6301 aaaaaatact tttttatata ctaaacactc actttatgtg agtgtttttt gtttaatgct
     6361 aaaatcattt ttttagatgt aattaatctt taataactat gtttgtattg taaatgtaat
     6421 gcataaaaaa tgcaagaaat tcaattgtca ccagaagaaa tagcacgcta ctcaaggcat
     6481 ctacttgtcg aagagtttgg tatggaaggc caactaagat taaaacaagc taaagtgcta
     6541 gtggttggtg ctggtggttt gggatgccca ttgttattgt atttagcagc tgcaggcgtt
     6601 ggcactattg gaattataga ttttgataca gtggaggaaa gtaacttgca gcgacaaata
     6661 ttatacacca tcgatgacat tggaaagcca aaagtagatg tggcgaaaaa aaaattatta
     6721 gcaataaatc cgtatataca cataattact tatcagcaac aattaagtgc aaaaaacgca
     6781 ctcgagatat tttcatcttt tgatatcgtt gctgatggaa ccgataattt tgtaacacga
     6841 tatttagtca acgatgcttg tgtatttagt gatattccaa atgtgtatgc ctccatctta
     6901 caatttgaag gacaagtatc tgtgtttaat tataaagaag gaccaaatta tagagatatt
     6961 tttcctgtgc caccagcacc gaacacaata ccaaattgtg cagaagcagg cgtgcttggt
     7021 gtattgccag gaattattgg aagtatgcaa gccaatgaag taataaaagt tatttctggc
     7081 ataggcacac ctttaagtgg tcgcctattt atatttgatg cattgcattt ttccagtaaa
     7141 atatttacta tttcaaaaga taaaaacaat cctttaactg gcgacaatcc aacaataaaa
     7201 gcacttactg atgttgattt attttgcgga gcaacatcaa gtattacaaa tgaaattagt
     7261 gtgcagcaat taaaggaaat gttcgatact aaggaagata ttcaactaat agatgtgcga
     7321 gagcattatg aatataatca agttaattta ggtggaattt gtattccatt gcatcagtta
     7381 aaacaatcaa tggatttaat ttcaaaagag aaaaaagtaa tagtgcattg taagactggt
     7441 atgcgtagcg ctaaagcagt gcagctttta ttagataatg gatatcgtaa cgtgtataat
     7501 cttaatggtg gcattattgt atacgcaaaa gaaatagaca atcaattgcc attgtattaa
     7561 atattaattg tcgcacttat gatttgaatt cagctgacta gaattcaaca cactaaataa
     7621 ttaaaaatac atattgttat tttctgtaaa actgtaaaaa ttgttatgcg caataatgat
     7681 atgatcgaca attgtgatgt caaataattt ccctgcttct acagcttgtt ttgttaatct
     7741 gatatcttga tcgctcggat tcaaattgcc tgatggatga ttatgacata gaataatgcc
     7801 actcgctaat tcttttatgg caatattaaa aacgattttc atgtcaacca ctgtacctga
     7861 aacaccacca gtgctaattt tatgtttcga aataaaacga ttatttcttt ttaagcaaat
     7921 aatccaaaat tcttcataag gcaagtcaga caaaattggt gaaataattt cgaatgctgc
     7981 cttgcaggaa ataataccgt cttgtggttt actttctgca atcgttcgtc ttctgccaag
     8041 ttctaaggca gcagcaatag atatggcttt agcctcacca atgcctttaa actgcattag
     8101 aaaatcgagt ggttttctac ctaattcaaa taaatcattg tgtactgcag cgaggatttg
     8161 tctggatagc tctactgcgc tattttctgt atttcctgcg ccgataataa tggcgagtaa
     8221 ttcagcatcg gataagctgt gttttccttt taatagaagt ttttcgcgag gtctatcttc
     8281 ttctgcccac gattttatac tgagctttgc tggtgtgtac ataaaataaa aattggtgta
     8341 ccattgagat acaccaatta cttattttcc ataaaaaaaa tggaattatt tttttaacgc
     8401 atttaaatgt ctcattacag cagactttaa atgactagct ttgtttttgt ggatttggtt
     8461 tcttttagcc aatttatcta ccattgcaac tactgcagac atagattttt ccgcttcgtt
     8521 tttatctgat aaacctaagt atttgcgcaa tgcatttctt gtggttttac catgataacg
     8581 gttttccaat ctgcgtttta ccgtctgtct gattcttttc tttgctgact tatgatgtgc
     8641 catttctata aatttgagtg tgcaaagata atcaatcatt tagattaaaa aaatgaattt
     8701 taggttaatt gtgtactttt ttattctttt tattgattta atctatcata aaataagctt
     8761 ttttaatgtg attataagct aaaaagcacg atatttgtcg tctgaatata atatatacat
     8821 aatatggaag atttatccat cttgtcgaat gcacatccaa gttatatcga tgctctctat
     8881 aaagactact taaaagacca aaattccgtt gatgctgaat ggaaaaaatt ctttgctggt
     8941 tttgatttag cttataaata tggtgaaaat ggcgctctaa ccagcgaaaa tgctaatgct
     9001 gacacacaag ccacaaatgc caatgttgtg tctccaaaag aatttaaagt actaaaactt
     9061 atttcaggat atcgaaataa agggcattta gtagctgtaa cgaatccgtt gcgacctcga
     9121 aaagacagac atgccgactt agatttgcac ttttttggtt tagaagaatc tgatttagat
     9181 gttgagtttt atgctggtaa agaaataggc atcggaagag ctacattgcg taccattatt
     9241 gagcgattac aaaaaatata ttgccgcacc ataggctggg aatacaatta tattttagat
     9301 agagatgaac gtgcttggat gcgcgagcaa atcgaacatg gttatattgc ttatgaacat
     9361 ccaattgaaa agaaaaaaag aattctgtct aaactgaatg atggtgaggt tttagaacga
     9421 tttttaggaa caaaatatat tggtcaaaaa cgattttctt tggaaggcgg cgagtccact
     9481 attcctgcat tagacgctat gattaacatc gctgcaaatc atggagtaga agaaatagtt
     9541 ataggcatgg cacatcgcgg ccgtttgaat gtgttgacaa atatcgttgg taaaacatac
     9601 gaacaagtat tttcagagtt cgaaggtgtt gcaccaaatg atttttctag tggcaccggc
     9661 gacgtgaaat atcacttagg ctattcatcg caatatgaca caatggacgg aaaagaagtg
     9721 tatattaatt tactacctaa tccatcgcat ttagaggctg taaatccagt agtgcaaggt
     9781 tattgtcgtg ctaaagcaga tgcaatttat gagtctgact acgataaaat tttaccgatt
     9841 actatacatg gcgattctgc ggttgcagga caaggtattg tgtatgaagt attgcaaatg
     9901 caatcgctaa aaggctatac agttggcggc accattcatt ttgttatcaa taaccaaatt
     9961 ggttttacaa cagattttga cgatgcgcgt acttctaatt attgtacctc tattgcatct
    10021 acgattcaag cacctgtatt tcatgtaaac ggtgatgatg ttgaatgcgt aacgtacgtc
    10081 gcagagctcg ccgcagaata tcgtcaacgt tttaataaag atgtgtttgt ggatatggtg
    10141 tgttacagaa aatggggaca caatgaaggc gatgatccat catacacaca gcctcagatg
    10201 tataaaataa ttaaagacca tgtaggtgtt cgtgatttat accaaaagaa actatacgat
    10261 tggggcgtgg cggaagcaga gtttgctaaa aaaatggaag aagaattttg gcaagaactg
    10321 caagcgcgat taaatgatta caaacaacaa gaaaaaaaat ataaaccaca atccacagaa
    10381 ttagcatgga aagcattgcg taggtcaacg aatgcagatt ttgaacaatc tccagaaaca
    10441 aaaatttcaa aagataatct tgtaaaaatt gtaaaagcat tggctaaaac tcctgaaggt
    10501 tttaagccat tgaaaaaaat acaacaatat ttagatcaac gtcgtgtgtt gatgcgtgaa
    10561 aataatagtg ttgactgggc agctggcgaa ttgttagctt atggttccat tttattagat
    10621 ggcaaagatg tgcgtttgag tggcgaagat gtgaaacgag gcacgttctc acatcgtcat
    10681 gcttgtgtat atgacgaagt aacgggtcaa gaatacaatc gattaaattt tattgaagaa
    10741 gatggcaagc aaggtacttt ccatatttat aattcccact tatctgagta tggcatactt
    10801 ggttttgaat ttggatattc cttgccatcg cctgcaccat tgaccatttg ggaagcacag
    10861 tttggcgatt tcgataatgg cgctcaaatt atcatcgacc aatttattgc ttcttgcgaa
    10921 acaaaatgga acagacaaag tggcttggtt ttattattgc cacacggtta cgaaggtgct
    10981 ggtccagaac atagttctgc acgcttagaa agatttttac aattgtgtgg tgaaaataac
    11041 atgattgtga ccaacatcac ctctccagct aatttcttcc atgctattag aagacagtta
    11101 acttggtcat tcagaaaacc aatgattaat atgtcgccaa aatcattact gcgtcatccg
    11161 aagtgtgtag atccaatttc gaaaatctat gaaggaaaat tccaagaagt gattgatgat
    11221 gaaaatacac ttgacgatgt gagtgtaaaa aaagtattat tctgtaccgg aaaaatttat
    11281 tacgatttat tagcaaagaa agaagcagat aatagaacag atgttgcgat tgtacgtgta
    11341 gaacaattgt atcctgttgc cgataaacaa ttagatgcca taattaaaaa atacagcaag
    11401 gcaacttatc attgggtgca ggaagaaccg attaatatgg gtgcttattc atttttatta
    11461 ttgaattaca gcaaagcaaa atatttacaa tgcattgcac gaccacaggc tgcatcgccg
    11521 gctacaggtt tcagcaaact gcacgagaaa gaacaaaaag cattggtaga attggcgttt
    11581 ggataatata tatcttggaa aataaattca cgcttattgt agttaaacaa taactttgta
    11641 aaaccaactg tatataagca tcatgcaacc gaaaacgata caagacatac agaattattt
    11701 taatgaacac gatttgttca acaaatatgg tttaactaaa attggcgtgt ttggctcgtt
    11761 tgcgcgcggt gaaaaattca atgatatcga tttatatgta gaagatgaca tagaactagg
    11821 aaaagctgct gaattaaaag tgttattaga aaacgattat aacttgcaaa ttgatttagt
    11881 tttgaaagct aaagaaaata agcttattat gttttatgca aaaaaagaca tgaaatacat
    11941 ttatgccaat tagagaagat tatattattg ttttaagtat tctgcaatgt gtaggaaaaa
    12001 cagttcattt tacaaacaaa ttcaatactg ctgatatttt tttcgaacat aataatcaat
    12061 taaactattt ggcatctcta acactacttg caaatatcgg cgaacatgca aataaactat
    12121 ctgaaaattt taagaaggaa catagtaaaa ttaattggaa acaaattaaa ggattaagaa
    12181 attttatagt tcacaattac gaaggagttg actatattat cttgtttaat accataaaaa
    12241 atgacttgcc aaaactgaaa ttagaattag agaattattt aaaagaatca attctgtcaa
    12301 attgtattga taaagaaata tttgtagaat tagctttaga taacccattt tatgagttta
    12361 taaattttga aaatataatt gaacaatgag caaaatagaa ataaaagtac caagcattgg
    12421 cgaatctgta acagaagtaa ccttgtcttc ctggttagtg gaagatggcg attatgtaaa
    12481 attagatcaa gctttagcag agtttgaatc cgataaagca acctttgaaa tgccatcgga
    12541 agcaacaggc gtcattactc gcgtagccaa agaaggagat gatgtgaaaa ttggtggtct
    12601 tgttgcctac attgatacag atgcagcagc gcccgaaaaa tcaacaggca gtagccaaga
    12661 gtcagtagag aaaacagagg aaattaaagt ggaagataaa gtagaggaaa atctgaaatc
    12721 tgaaatttcc aatctgcaaa ctaattacgc aacaggcact ccatcgccag ccgcgaaaaa
    12781 aatattagat gaaaaaggta ttgaaactac ggaagtaaaa ggcactggca tagatggaag
    12841 aataactaaa gaagatgcag aaaaagcaca gaagagtgaa aaggtattag tgaagagtga
    12901 agaaaatgaa acagagaaaa aaacaaatat ccaacaacct ataacaagta atttttcacg
    12961 aagtgaaaga attgagaaaa tgacgcgtat gcgcaaa
//
LOCUS       DAOQQV010000070        12960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1649_length_12960_cov_6.383417, whole genome shotgun sequence.
ACCESSION   DAOQQV010000070 DAOQQV010000000
VERSION     DAOQQV010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12960
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1649_length_12960_cov_6.383417"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLL59_08155"
     CDS             <1..581
                     /locus_tag="PLL59_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23820.1"
                     /translation="ILSSNTNYTVTGTDANNCTGTAVATITVKPKPTTPVITQGHDTL
                     YSSINLAGASYDWYKNGVFHATTSTRFIRITQAGTYTLIIRNNGCSSISSTGFNATLT
                     AIIKNKLAIQLAIVPNPNNGAFDINIVSPAYKNYTLSMFTIAGQEIYTDEFNVHIGQN
                     NKRVSIPNLEKGIYLISIIGEEGTSTQTILVQ"
     gene            642..1361
                     /gene="hisA"
                     /locus_tag="PLL59_08160"
     CDS             642..1361
                     /gene="hisA"
                     /locus_tag="PLL59_08160"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HMY23821.1"
                     /translation="MLIIPAIDIIEGKCVRLSKGDYDTKKIYNDNPLEVAKEFEANGI
                     TRLHLVDLDGAKSGKVVNWKVLESIASKTKLHIDFSGGIKTKEAANQAFELGAAQITV
                     GSLAAKNPKEFVDWIWEFGEEKLILGADVIKDKIAIHGWQESSTKDIYEYLDFYFEEG
                     IDYVLCTDVSKDGMLQGPAIELYKDIIKEYEEIKLIASGGVSCMRDLEQLKEIGCYAA
                     IVGKALYENKISFSDLQSFLK"
     gene            1367..2119
                     /gene="hisF"
                     /locus_tag="PLL59_08165"
     CDS             1367..2119
                     /gene="hisF"
                     /locus_tag="PLL59_08165"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HMY23822.1"
                     /translation="MLTKRIIPCLDIKNGETVKGVNFVDLRSAGDPVELAKQYVIQGA
                     DELVFLDITATVEARKTLVSLVEKVAAAINIPFTVGGGISTIQDVDALLNAGADKVSI
                     NSSAVRNPNLIDEFSKQFGSQCIVVAIDTKLEDDWKVYVKGGREATPIYTKNWAREIQ
                     ERGGGEILLTSMNNDGTKKGFALDITKWCAENVAIPIIASGGAGNKQHFADVFSLAHA
                     DAALAASVFHFGEIPIPELKKYLKANEINVRI"
     gene            2138..2734
                     /gene="hisIE"
                     /locus_tag="PLL59_08170"
     CDS             2138..2734
                     /gene="hisIE"
                     /locus_tag="PLL59_08170"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HMY23823.1"
                     /translation="MQAGFSKNIDGLLPAIIQDAKTGKVLMLGYMNEESLQKTIDSNK
                     VTFFSRSKQRLWTKGEESGNFLELVSIKLDCDKDTFLIQANPVGAVCHTGSDTCWNEK
                     NEHSFSFLYELENIIADRKINPIENSYTNLLFNKGINKIAQKVGEEAVEVVIEAKDNN
                     DHLFKNETADLLFHLLVLLREKDISLADVITVLQERHK"
     gene            2830..2988
                     /locus_tag="PLL59_08175"
     CDS             2830..2988
                     /locus_tag="PLL59_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HMY23824.1"
                     /translation="MSIWRVLLSILFPPLAVYDKGCGSITITFILTLIGWVPGVIAAL
                     VILNKNES"
     gene            complement(3204..3638)
                     /locus_tag="PLL59_08180"
     CDS             complement(3204..3638)
                     /locus_tag="PLL59_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HMY23825.1"
                     /translation="MKPTYNLERFIEAQEEDYGIALREVKQGAKVNHWMWYIFPQIAG
                     LGNSSTAKHFAIMDITEAKLYLEHPVLGKRLAEISEALLRIPHNSAYTIFGTPDDLKL
                     LSCMTLFEAIDSQYKDIFKRIIDKYYNGRRDSKTLQLLKFNL"
     gene            complement(3929..4299)
                     /gene="ssrA"
                     /locus_tag="PLL59_08185"
     tmRNA           complement(3929..4299)
                     /gene="ssrA"
                     /locus_tag="PLL59_08185"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4425..5642)
                     /locus_tag="PLL59_08190"
     CDS             complement(4425..5642)
                     /locus_tag="PLL59_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMY23826.1"
                     /translation="MPKISQKGNSMPASPIRKLAPFAEQAKKNGTHIYHLNIGQPDIE
                     TPHQFWDAIKKLDRKVLEYSPSNGFEEVRNKYAGFFQNRYGLKNLQADDLLVTTGGSE
                     ALMFTLFSILDSDDEIIIPEPLYANYIGFSKSGNINVRPIPTSFEQAFALPSIDEFEK
                     VINEKTKAILVCNPNNPTGYVYSDDELNRLKEIVLKHDLFLISDEVYRDFIYGETKHY
                     TSILSYPELEQHAIVVDSISKRFSACGARIGMVATKNNEVLQTVLKFAQQRLSPPTVE
                     QLGAIAMFDVEDNYFTKVNNEYRKRRDTLVEGLNAIDGVHCHTPGGAFYCIVQLPVKD
                     TDDFCQWMLSDFSYEGETVMMAPASGFYATEGRGKNEVRIAYVLNCVDLKKAIKCLEE
                     GLKAYNNNSSTRF"
     gene            complement(5715..6146)
                     /locus_tag="PLL59_08195"
     CDS             complement(5715..6146)
                     /locus_tag="PLL59_08195"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMY23827.1"
                     /translation="MKNMFILTTVVLLLNVFVFAEPPVASKIKFTKLEHDFGNLAQGK
                     PVTYDFEFTNVGNEPIILENVKASCGCTTPTWTKEPVMPQKKGTIKAQYNMAREGAFR
                     KSITVTTKDGENITLYISGNAVPQEQGVNKAEGNMLGGSVD"
     gene            6347..8782
                     /locus_tag="PLL59_08200"
     CDS             6347..8782
                     /locus_tag="PLL59_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMY23828.1"
                     /translation="MTLISSNVLITKGVSQHVSFEEFKDEVLSDYRLACISRETSLTG
                     RKEVLTGKAKFGIFGDGKELPQIALAKVFENGDFRSGYYRDQTFALAAGIVTVEQLFG
                     QLYANPSLEEDPHSGGRQMNNHFATRNLHSDGKWKDIKNQKNSAADNSPTASQMIRAL
                     GLAMASKKYRELQTQIGENGFSNAGNEICFATIGDASTSEGLFWEVVNAGGVLQVPLI
                     ISVWDDGYGISVPKKYQTTKGSISQVLKGFQRSGDSNGYDIYVVKGWNYPQLVSTYKM
                     AADKTRKTHIPAIVHVEELTQPQGHSTSGSHERYKSAERLQWEKEHDCNVQFRKWILQ
                     NKIASEAELNEIESEAKKYIAEQKKLAWKKFHEPILDELKEATNIIKKIAETSAYKEQ
                     LDELEKEIRYSIDPMRKDVFKTISDVLSLVRDEQINAVDELKKWRNIHTQINDERYSS
                     YQHTNSLYSPLRLPIIQAQFEDKPSIVSGYQVLNACFDANFSRDAKIIAFGEDVGKIG
                     DVNQAFAGLQAKYGIERIYDTGIREASIMGEGIGLAMRGLRPIAEIQYLDYLLYGLQP
                     LSDDLACLSYRTKGGQKAPLIVRTRGHRLEGIWHTGSPMQMILGALRGMHVCVPRNMT
                     QAAGFYNLLLRGDEPALVIECLNGYRLKEKMPLNPGEFTVPLGVPEILKQGTDITLVT
                     YGSCVRIAQEANISLQKAGISVEIIDVQTLLPFDTYGVIGTSIRKTNRVMFLDEDYPG
                     GGTAFMLQQVLEKQDIFRYLDSEPVTLTAKEHRGAFGSDGDYFCKPNAEDVIHTINKI
                     MYEVNPKRFPMMW"
     gene            complement(8805..10136)
                     /locus_tag="PLL59_08205"
     CDS             complement(8805..10136)
                     /locus_tag="PLL59_08205"
                     /inference="COORDINATES: protein motif:HMM:NF020129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HMY23829.1"
                     /translation="MKVGIEAATFFIPDLFLEISELAEHRGIDNNKLLLGLGLQKMAI
                     IDTNEDTATLAAEALLRLFTDYEIHPKEIARIYLGTESALDAAKPTATFAVQMVETQL
                     ATQFGERCFKNCDVLDMTFACIGGIDALHNALDFVRINPNKKAIVLAADYAKYELNSG
                     GEYTQGAGAIALLISATPNLIEIENYFGVGMSSVYDFFKPRRHVDKDKLINAPENYPE
                     QIEIFSDEPIFDGQYSNECYKQRIQEAFYNFKSEKLIHTNVISSWEYIIFHLPYAFHG
                     KRIFAEIFASENNIPCSTKDEVKAVLLSDDYKQFINNKIEPSQRASSEVGNLYTASIF
                     MALFSTLQTAFLENKELTGKKIGFFAYGSGSKAKVFEATLHTNWKSVVKNWNLFEALN
                     QRTAINFETYIQLHKKALKKPVNTIQKGFKLEKIETDIQELNGARYYKYHV"
     gene            10295..10909
                     /locus_tag="PLL59_08210"
     CDS             10295..10909
                     /locus_tag="PLL59_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HMY23830.1"
                     /translation="MKDYYKILGLPYDASLHDVKKAYRKLAFIYHPDLNKNNIIAEER
                     FKEISEAYSVLSNKDRAYQYRILFVEYLQSIKINGDKNTRQQHTFTTHDRPRAANGKA
                     SDRSVNWNVFNVGAIFLFSIFIFFMLVSAFKEMRKNENEHARLTEYISKEIKPSKKTY
                     KITKDEYYAMLAQEFAASNDSTLYKIENVDSMMQVIDSLNILNY"
     gene            complement(10914..11627)
                     /locus_tag="PLL59_08215"
     CDS             complement(10914..11627)
                     /locus_tag="PLL59_08215"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HMY23831.1"
                     /translation="MTRLSVNLNKVALLRNSRGSNFPDLIQVAKDCERFGAQGITVHP
                     RPDERHCKFSDLPLLKEICTTEFNIEGFPNEHFIAEVIKVKPHQCTLVPDDPNQLTSD
                     HGWNTIEKKDFLQDVVNELKRNNIRVSLFIDADKKLIEGAANVGADRIEYYTGPYAKK
                     YSTNPEAAIKSIKETCVIAQQLGLGINAGHDLNLENLRYLKDNLCQLDEVSIGHALIA
                     DALYLGLENTIRLYLNKLQ"
     gene            complement(11631..12632)
                     /locus_tag="PLL59_08220"
     CDS             complement(11631..12632)
                     /locus_tag="PLL59_08220"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HMY23832.1"
                     /translation="MENIAVLQEFLKTSRNIIITTHQKPDGDAMGSSLALYNYLQNIG
                     HQVRVISSTEFPAYFSYLPNCDEVWNYLENPSLSQIAIESAELIFCLDFNDLGRIEPL
                     DSHIKNNKNAKIVLLDHHLHPKIEATWSLHDVKACSTCELVYRFLSLFDANYIPTKEV
                     ATCIYTGILTDTASFSNGATNKKALEITAHLLDCGLNIIDVQEQLNQNGREEKLRFLG
                     NALFRNTIIREDIGIGIIVVDKKDAYRFNLQTGDTEGLVNYPLSIKNVKVSILVKQEP
                     KIIKLSFRSKGEYSVEKICREHFEGGGHRNASGGRSFLSVEQTIDKIISIFEKEHLV"
     gene            12758..>12960
                     /locus_tag="PLL59_08225"
     CDS             12758..>12960
                     /locus_tag="PLL59_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HMY23833.1"
                     /translation="MATNRTFTMIKPDAVEANNIGPILAMMTEAGFKVVALKYTKLTQ
                     EQAGLFYEIHKERPFYGELVEFM"
BASE COUNT         4282 a         2126 c         2284 g         4268 t
ORIGIN      
        1 ctatactaag tagtaatact aattatacgg ttacaggaac tgatgcgaat aattgtactg
       61 gcactgctgt tgcaacaatt acagttaaac caaaaccaac tacaccagtt attacacaag
      121 gccatgatac attatatagc agcattaatc ttgctggcgc cagctatgat tggtataaaa
      181 atggtgtttt tcatgccacg acgagtacaa gattcataag aattacacaa gctggaacat
      241 atacattaat tataagaaat aatggatgta gttctattag ctcaacagga tttaatgcta
      301 cgctaacggc aattataaaa aataaattag caatacaatt ggcaatcgtt ccaaatccaa
      361 ataatggagc atttgatata aatattgttt caccagcata taaaaattat acactcagca
      421 tgtttactat tgcaggtcag gaaatatata ctgatgaatt taatgttcat atagggcaaa
      481 ataataaacg agtgtctatt ccaaatttag aaaaaggtat ttatcttatt tcaatcattg
      541 gcgaagaagg aacaagcaca caaactattt tggtgcaata gattttaatt tgttaaactt
      601 aaagactgta cattttgtgc agtctttttt atttttacaa gatgctaatt attccagcaa
      661 tagatattat cgaaggtaaa tgtgtgcgct tgtcaaaagg tgattatgac acgaaaaaaa
      721 tatataatga caatccttta gaagtagcaa aagagtttga agcaaatggt attacacggt
      781 tgcatttagt agatttagat ggtgctaaaa gtggaaaggt agtcaattgg aaagtattag
      841 aatccattgc ttctaagaca aaattacata ttgatttttc aggtggaata aaaacaaagg
      901 aagcagcgaa tcaggcattt gaacttggtg cagcacaaat tacagtcgga agtttggcag
      961 ctaaaaaccc aaaagaattt gtcgattgga tttgggaatt tggagaagaa aaattgatac
     1021 tcggtgccga tgttatcaaa gataaaatag ccatacacgg ctggcaagaa tccagcacta
     1081 aagatatcta tgaatattta gatttttatt ttgaagaagg tatcgattac gtattgtgta
     1141 cagatgtttc taaagacggt atgttacaag gtccagctat agaattatac aaagatataa
     1201 ttaaagagta cgaagaaatt aaattaattg caagcggtgg cgtttcttgt atgcgcgact
     1261 tagaacaatt gaaagaaatt ggttgttatg cagctattgt aggcaaggct ttgtatgaaa
     1321 ataaaatctc attttctgac cttcaatcat ttctaaaata attgcattgc tcaccaaacg
     1381 catcatacca tgccttgata tcaaaaacgg tgaaaccgtt aaaggtgtga attttgtgga
     1441 tctacgaagt gctggcgatc cagtcgagtt agccaaacaa tatgttatac aaggcgcaga
     1501 tgagcttgta tttttagaca ttacagcaac tgtcgaagcg agaaaaacct tggtgtcatt
     1561 agtagaaaaa gtagctgctg ctataaatat tccttttacg gttggcggtg gtatttctac
     1621 cattcaagat gtagatgcgc ttttaaatgc tggtgctgat aaggtttcta tcaattcttc
     1681 agcagtgaga aacccaaatt taatagatga gttttctaaa caattcggtt cacaatgtat
     1741 tgttgttgca attgacacaa agttggaaga tgattggaaa gtatatgtta aaggaggaag
     1801 agaagctacg ccaatatata ctaaaaattg ggcacgcgaa attcaagaac gtggtggtgg
     1861 cgaaatttta ttaacttcaa tgaataatga tggcactaaa aaaggatttg ctttagacat
     1921 tacaaaatgg tgtgcagaaa atgttgctat tcctatcatt gctagtggtg gtgcaggaaa
     1981 taaacaacat tttgcagatg ttttttcgct tgcacatgca gatgcagcgt tggcagctag
     2041 tgtttttcat tttggcgaaa ttcctattcc tgaattaaaa aaatatttga aagcaaacga
     2101 aataaatgtt cgtatttaaa aagacaattt tataactatg caagcaggtt tttctaaaaa
     2161 tatagatggc ttattgccgg caattattca agatgcaaaa acaggaaagg tgttgatgct
     2221 tggatatatg aatgaagaaa gtttacaaaa aacaatagat agcaataagg tgactttttt
     2281 ctcgcgtagt aaacaaagac tttggacaaa aggagaggaa agtggtaatt ttttagaact
     2341 tgtttcaata aaactagatt gcgataaaga cacctttttg atacaagcaa accctgtggg
     2401 agctgtgtgt catactggta gcgatacatg ctggaacgaa aaaaatgagc atagtttttc
     2461 atttttatac gaactagaaa atattattgc agatagaaaa attaatccaa tagaaaactc
     2521 atataccaat ttgcttttta ataaaggtat caacaaaatt gcacaaaaag taggtgaaga
     2581 agcagtagaa gttgtgattg aggcaaaaga caataatgac catcttttta aaaatgaaac
     2641 cgccgattta cttttccatc tattagttct acttcgcgaa aaagatattt cattggcaga
     2701 tgttatcact gttttacaag aaagacacaa gtaaacttat tctgttaatt tttctttaaa
     2761 ctaaattatt ttggaaccaa aatatgtttg gtatatgttt tgtatgtata atcaaaacaa
     2821 agcatagaaa tgagtatttg gcgagtatta ttaagcattt tatttccacc tttagcggtg
     2881 tatgacaaag gttgtggtag cattactatt acatttatat tgactttgat aggctgggtg
     2941 cctggcgtta ttgcggcact cgtcatttta aataaaaatg aaagctaatc tttcatagtt
     3001 ttttttaggg ttttatgttt ttggttaacc gttctgattg cagaacggtt tttgtttgct
     3061 cgctgcgctc gccctcgttg gtttgtaagc aacgaactac cctcactggt aataataaat
     3121 atattattat atctttaatc agtggaagtc gaaacggaaa gtggtttgtt ggttgaaaac
     3181 caacaaaggc aagggatgaa tatttaaaga ttgaatttca gtaattgcaa tgttttactg
     3241 tcacgccttc cattataata cttatcaatt attcttttga aaatatcttt gtattggcta
     3301 tcaatagctt caaataaagt catgcaggaa agtaatttta aatcatctgg tgtgccaaat
     3361 atagtatagg cagaattatg tggtattctt aataaagctt cgcttatttc tgcaagtctt
     3421 tttcccaaaa caggatgttc gagatataat tttgcttcag taatatccat gatagcaaag
     3481 tgttttgcag tactgctatt tcccaatcct gcaatttgag gaaatatata ccacatccag
     3541 tgatttactt tcgcgccttg tttaacctcc cgtaaagcaa tgccataatc ctcttcctgt
     3601 gcttcaataa atcgttctaa attataagtt ggtttcatag ataaatatta tttacttaat
     3661 gaataagtat actccagtgt cccaaactgg cgtaagaatg aggtatcgca atgtgcgttg
     3721 gcttcttgtg ccaaacttga atcttttgca aaaaaaaatc tttttctctt gctcgcaacg
     3781 ctcgccttcg ttggtttgta agcaacgaac tactaagcga ataaacaaca tatctaattt
     3841 attattggat ttaaatcaac aacgtatgga acttctttaa aataaaaaat accataagca
     3901 attgattata tattacttat ggtattagta gtggagctgg cgagatttga actcgcgtcc
     3961 ggacaaaacg tctataagct ttctacatgc ttagtactaa cttgattttc gaattactgc
     4021 aggcgttatt acaaaccgac aataatctta gtctctaaat ttcgcaacat agtcgagacc
     4081 attatgctgc tattccgatg agttgatgct cagtcgaaag gcatcggaaa agccgtttcg
     4141 aggagcaaag gccgagctta ataacctgta ctaagcagcc aaagcgaaag tattctcgcc
     4201 atataaaaag atttcgtgcg ttaaatttaa cgagccaaat tgcacaaagc tcggcatgct
     4261 tacctatcta ctatcctgcc gtcaattcct gtcaacccca atgtatgttg atgtttaaaa
     4321 atttagacag ttttttttaa gaactttatc tattgtaaca atagtatcta aaggatggtt
     4381 ccacaaagat aaaattgatt tccaaataaa taattatttc aagattaaaa tctcgttgat
     4441 gaattattgt tgtaagcttt taagccttct tctaaacatt ttatggcttt ctttaagtca
     4501 acacagttaa gcacataagc aattcgaact tcattttttc ctctaccttc agtagcataa
     4561 aaaccgctgg caggtgccat catcaccgtt tctccttcat aactaaaatc actcaacatc
     4621 cattggcaaa aatcgtctgt atcttttaca ggcaattgca ctatgcaata aaaggcgcca
     4681 cctggcgtat ggcaatgcac gccatcaatt gcgtttaatc cttctactaa tgtgtctcta
     4741 cgctttctgt attcattatt tactttagta aaataattgt cttcgacatc aaacatggct
     4801 attgcgccta attgctctac tgttggcgga cttaaccttt gctgtgcaaa tttcaaaact
     4861 gtttgtaata cttcattgtt ttttgttgcc accataccta ttctggcgcc acaagcacta
     4921 aatcttttgg aaatagaatc tacaactatt gcatgttgtt ctaattcagg atagctcaat
     4981 attgaagtgt aatgttttgt ttcaccataa ataaaatcgc gatatacttc atcgcttatt
     5041 aaaaacaaat catgctttaa tacaatttct tttaatcgat ttaattcatc atcactatac
     5101 acataacctg tcggattatt aggattacaa accaatattg cttttgtttt ttcattgatt
     5161 actttttcaa attcatcgat ggacggcaat gcaaatgctt gctcaaagct cgtaggaatt
     5221 ggtcttacat tgatatttcc actttttgaa aatccaatat aattggcata taaaggttct
     5281 ggaatgatga tttcatcatc ggaatctaaa atcgaaaaca aagtaaacat taatgcttcc
     5341 gaaccgccag tagttactaa caaatcatca gcttgcaaat ttttcaagcc atatctgttt
     5401 tgaaaaaagc ctgcatattt gttgcgcact tcttcaaagc catttgatgg tgaatattcc
     5461 aatacttttc tatccaactt tttgatggca tcccaaaatt ggtgtggtgt ctcaatatca
     5521 ggttgaccaa tattgagatg ataaatatgt gtgccatttt tcttagcttg ctcagcaaat
     5581 ggagcaagtt ttcgtattgg cgatgctggc atggaattgc ccttttgtga tatttttggc
     5641 ataattattc tacaaatgta aaaactctgt gctaaatgca cagagttttt atataaagat
     5701 atgttgaaga agtattaatc gacacttccg ccaagcatat tgccttctgc tttattaacg
     5761 ccttgttctt gtggaacggc atttcctgaa atatataagg taatattttc accatctttt
     5821 gtcgtaacag taattgattt tctgaatgca ccttctctag ccatattata ttgcgcttta
     5881 atcgtacctt tcttttgtgg cattaccggt tcttttgtcc atgttggtgt cgtgcaacca
     5941 catgatgctt ttacattttc taaaattatc ggttcgttgc caacattggt aaattcgaaa
     6001 tcataagtaa caggcttgcc ttgtgctaaa tttccgaagt cgtgttctaa ttttgtaaat
     6061 ttaattttag atgccacagg tggttcggca aaaacaaaaa catttaataa aagtactact
     6121 gtggtaagga taaacatatt tttcatatct ataattttgc ttagctaaga aataattata
     6181 gcaaagtgct ttgcttattg caaatctcac aaaaaatata ccaaatacct gctttgtgga
     6241 tatattttaa ctacacatta acaactttac caaaatgtga ataatcttaa gattatttca
     6301 gagaataagt gtaatttcga tataactttg cacaataatt catagtatga cattaatatc
     6361 ttctaatgta ttgattacta aaggcgttag tcagcacgta tctttcgaag agtttaaaga
     6421 tgaggtactc agtgattatc gtttagcttg cataagccgt gaaaccagct tgacaggcag
     6481 aaaagaagtg ctgacaggta aggcaaagtt tggaattttt ggcgatggaa aagaattgcc
     6541 acaaattgca ttggccaaag tttttgaaaa cggagatttt cgttctggat attatcgcga
     6601 ccaaacattt gcattagcgg ctggcattgt tactgttgaa caattatttg gtcaattata
     6661 tgccaatcct tccttagaag aagatcctca ttctggtggc cgacaaatga acaatcattt
     6721 tgcaacgcgc aacttgcatt ctgatggcaa atggaaagat attaaaaatc aaaaaaattc
     6781 agcagcagat aattctccta cggcctcaca aatgattaga gcacttggct tggcaatggc
     6841 ttctaaaaaa tatagagaac tgcaaacaca aattggtgaa aatggattta gcaatgcagg
     6901 caacgaaatc tgttttgcca cgattggtga tgcttccact tcagaaggtc ttttttggga
     6961 agttgtgaat gccggtggtg tcttgcaagt gcctttaata atttccgttt gggacgatgg
     7021 ttatggtatt tctgttccta aaaaatatca aactacgaaa ggcagtattt cgcaagtgct
     7081 taaaggattt cagcgctctg gcgatagcaa tggctatgat atttatgtag tcaaaggttg
     7141 gaattatcca caactcgttt caacttataa aatggctgcc gataaaacta gaaaaacaca
     7201 tattccagcg attgtgcatg tcgaagaatt aacgcagcca caaggacatt ccacatctgg
     7261 atcgcatgaa cgctataaat ctgcggagcg actccaatgg gaaaaagaac acgattgcaa
     7321 tgtgcagttt cgcaagtgga ttttacaaaa caaaatcgct tctgaagcag aattaaatga
     7381 aatagaatca gaggcaaaaa aatacatagc tgagcaaaaa aaattagctt ggaaaaaatt
     7441 tcatgagcca attttagacg aattaaaaga ggcgaccaat atcattaaga aaattgcgga
     7501 aacatctgca tataaagagc aattagatga attagaaaaa gaaattcgtt atagcataga
     7561 tccaatgcgt aaagatgttt ttaaaacaat ctccgatgtt ttatcattgg taagagatga
     7621 acaaataaat gcggtagatg aattaaagaa atggcgaaat attcatacgc aaattaatga
     7681 cgaacgttat tcatcttacc aacataccaa ttctttatac tcacctttaa gattaccaat
     7741 tattcaagct caatttgaag ataaaccatc tattgtaagt ggttatcaag tgttgaatgc
     7801 ttgttttgat gctaacttta gcagagatgc aaaaataatt gcctttggtg aagatgtcgg
     7861 aaaaattggt gatgtcaacc aagcatttgc tggcttgcaa gcaaaatatg gtatagagcg
     7921 aatttatgat acaggcattc gagaagcgag catcatgggc gaaggcattg gattggccat
     7981 gcgtggtttg aggccaattg cagaaataca atatttagat tatttactgt atggtttaca
     8041 accattgtct gacgatttag cctgtttaag ttatagaacc aaaggcggac aaaaagcacc
     8101 tttaattgtt cgaactcgtg gtcatcgttt agaaggcatt tggcatacag gttcaccgat
     8161 gcaaatgatt ttaggtgcct tacgtggcat gcacgtatgt gtgccgagaa atatgacaca
     8221 agcagccgga ttctataatt tacttttaag aggagatgag ccagcattag tcatcgagtg
     8281 cctaaatggt tatcggctga aagaaaaaat gccactaaat ccaggcgaat ttacagtgcc
     8341 gctcggtgtt cctgaaattc tgaaacaagg cacagatatt actttagtga cctatggttc
     8401 atgtgtgcgc attgcgcaag aagccaatat tagcttgcaa aaagcaggca tttctgtaga
     8461 gattatagat gtacaaacgc tattgccttt tgatacttat ggtgttattg gcacttcgat
     8521 tcgcaaaacc aatcgcgtaa tgtttttaga cgaagattat cctggtggtg gaacagcatt
     8581 tatgttgcaa caagtgttgg aaaaacaaga tattttcaga tacttagaca gtgagccagt
     8641 aacattaaca gcaaaagagc atagaggtgc ttttggtagt gatggtgatt atttttgcaa
     8701 accaaatgca gaagatgtca ttcatactat caataaaatc atgtacgaag taaacccaaa
     8761 aagatttcca atgatgtggt agcgatattt ataagaatgg agaattatac atgatattta
     8821 tagtatcttg ctccgtttaa ttcttggata tctgtttcaa tcttttcgag tttaaatcct
     8881 ttttgtatcg tatttacagg tttttttaat gcttttttat gcaactgaat atatgtttca
     8941 aagtttatag ctgttcgttg gtttaacgct tcaaaaagat tccaattttt tactactgat
     9001 ttccaattgg tatgcaatgt agcttcaaaa accttggctt ttgaaccact tccgtaagca
     9061 aaaaatccaa ttttctttcc tgttaattct ttgttttcta gaaatgctgt ttgcaaagta
     9121 gaaaataaag ccataaaaat ggacgctgta tatagattac caacttcaga tgatgctcgt
     9181 tgtgatggtt caattttatt attaataaat tgtttataat cgtcgctgag taaaacagcc
     9241 tttacttcat cttttgtgct acatggaatg ttattttccg aagcaaatat ttctgcaaaa
     9301 attcgtttgc catgaaatgc gtatggtaaa tgaaaaatga tatattccca agaagaaatt
     9361 acattcgtat gtattaattt ttctgatttg aaattataga aagcttcttg aatacgttgt
     9421 ttataacatt cgtttgagta ctgtccatca aaaattggtt catctgaaaa aatttctatt
     9481 tgctctggat aattttcagg agcatttatt agcttgtctt tatcaacgtg tctccttggt
     9541 ttgaaaaaat cataaacact gctcattcct acaccaaaat agttttcgat ttctattaaa
     9601 ttaggagttg cagatattag aagtgcaatt gcacctgcgc cttgtgtgta ttcgccaccg
     9661 ctatttaatt catattttgc ataatccgca gcaagcacta tcgctttttt atttggattt
     9721 attcgaacaa aatctaacgc attatgtaag gcatcaatgc cgccaataca agcaaatgtc
     9781 atgtctaaaa catcacaatt tttaaagcat cgttcaccaa attgtgttgc taattgagtt
     9841 tctaccattt gcactgcaaa agttgctgta ggtttagccg catctaatgc actttctgta
     9901 cctaaataaa tgcgcgctat ttcctttgga tgaatttcat aatcagtaaa caaacgcaac
     9961 agcgcttccg cagcaagtgt agctgtatct tcgttagtgt caataatggc cattttttgt
    10021 aagcctaaac ctaacaagag tttattattg tcgatgcctc tgtgttcggc taattcgctt
    10081 atttctaaaa ataaatcagg tataaaaaaa gttgctgctt ctattccgac tttcataagt
    10141 atgtactaaa tttttacgaa aataatttct tttaatggca tattaaatca aatatttata
    10201 ataaattgcc tagcatcaga tttataatag aaccttaatt atttattgtt ataccagttt
    10261 cattcaacgt tcgttattta taatattatt ttcaatgaaa gattattata aaatattagg
    10321 attaccttat gacgcttctt tacacgacgt aaagaaggct taccgcaaat tggcatttat
    10381 atatcatcca gatttaaaca agaataatat tattgcagaa gaacgcttta aagaaatttc
    10441 ggaagcatat agtgtattat caaataaaga tcgtgcttat cagtatcgga ttttatttgt
    10501 ggaatattta caatctatta aaattaatgg agataaaaat acgaggcaac aacacacatt
    10561 taccacacat gacagacctc gagcagcaaa cggcaaagca agcgaccgtt ctgtaaattg
    10621 gaatgttttt aatgtaggtg caattttttt attttccatt tttatttttt ttatgcttgt
    10681 gagtgcattt aaagaaatga gaaaaaatga aaatgagcac gcacgtctca cagaatatat
    10741 ttcaaaagaa ataaaaccaa gtaagaaaac ttacaaaatt actaaagacg aatattatgc
    10801 aatgctggcg caagaatttg cagcttcaaa tgattctact ttatataaaa ttgaaaatgt
    10861 agattctatg atgcaggtga tagattcgtt aaatattcta aattattaat gtcttactga
    10921 agcttgttta aatacaaacg tattgtattt tctaatccaa gatatagtgc atcagcaatt
    10981 aacgcatgtc caatggatac ttcatcgagt tgacagagat tgtctttcag atatcgtaga
    11041 ttttctaaat tcaaatcgtg tccagcattg atgccaagtc ctaattgctg agcaatgaca
    11101 catgtctctt taatagattt tatggctgcc tctggattgg tgctgtattt ttttgcatat
    11161 ggtcctgtgt aatattctat tctgtcggca ccaacatttg cagcgccttc tatcaatttt
    11221 ttatcagcat caataaacaa agaaacgcga atattatttc ttttcaattc attgacgaca
    11281 tcttgcaaga agtctttttt ttcaatggta ttccagccat gatctgaggt gagttgatta
    11341 gggtcatcag gaactaaagt gcattgatgt ggttttactt tgattacttc tgcaataaaa
    11401 tgctcattag gaaatccttc tatattgaat tctgtagtac aaatttcttt gagcaagggc
    11461 aaatcagaaa acttacaatg tcgctcatct ggtcttggat gtacagtaat accttgcgct
    11521 ccaaaacgct cacaatcttt tgctacttga attaagtcag gaaaattgct gccacgtgaa
    11581 ttacgcaata aggctacttt atttaggttt accgaaagac gtgtcatgaa ttatactaaa
    11641 tgttcttttt caaaaatgct gattattttg tcaatagttt gctcaacact taaaaacgag
    11701 cgaccgccag aagcattgcg atgtccgcca ccttcgaagt gctcgcgaca aattttttct
    11761 actgaatatt cacctttgct tctaaaagaa agtttgatga tttttggttc ttgtttaact
    11821 aaaatagaaa cctttacatt tttaatgctc aaaggataat ttactaatcc ttccgtatcg
    11881 cctgtttgta aattaaatcg ataagcatct tttttatcga caacgataat gccaattcca
    11941 atgtcttctc tgataatcgt atttctaaaa agtgcattgc ctaagaaacg caatttttct
    12001 tcgcgaccgt tttggttgag ttgctcttgc acatctatga tatttaagcc acaatccaat
    12061 aaatgtgcag taatttctaa tgctttttta ttggtcgcac catttgagaa gctggctgtg
    12121 tctgtaagta tgcctgtata aatacaagtt gctacttctt ttgttggaat ataattagca
    12181 tcaaataaag ataaaaaacg ataaaccaac tcacaagtcg agcaagcttt tacatcatgt
    12241 aagctccaag tggcttctat ctttggatgc aaatgatggt cgagtaatac aatttttgca
    12301 tttttgttat ttttaatatg tgagtcaagt ggctcaattc gacctaaatc attaaaatct
    12361 aaacagaaaa taagttcggc agattcgata gcaatttggc ttaaactcgg attttccaaa
    12421 taattccata cttcatcgca attaggtaaa taactaaaat aagctggaaa ctcagtcgat
    12481 gaaattacgc gaacttgatg tccaatattt tgtaaataat tatacaatgc caatgaagaa
    12541 cccatagcat caccatctgg tttttggtgc gtggtgatga taatatttct cgatgttttt
    12601 aagaattcct gtagaacagc tatattttcc attgctacaa agatactaca tctggatatg
    12661 aaaatgaaga atgtgctaat tacattaatt tatagtaaat ctttcaactt ataactaaat
    12721 gattataagt tataattaat tttgcaaaaa aaatacaatg gcaacaaaca gaacttttac
    12781 tatgattaag ccagatgctg tagaagctaa caacatcggt ccaattttag caatgatgac
    12841 agaagctggt tttaaagtag tcgctttaaa atacacaaaa ttaactcagg agcaagctgg
    12901 tttattttat gaaattcaca aagaacgtcc attttatggc gaattagtgg agtttatgag
//
LOCUS       DAOQQV010000071        12873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1665_length_12873_cov_6.328132, whole genome shotgun sequence.
ACCESSION   DAOQQV010000071 DAOQQV010000000
VERSION     DAOQQV010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_12873_cov_6.328132"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..7598)
                     /locus_tag="PLL59_08230"
     CDS             complement(<1..7598)
                     /locus_tag="PLL59_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23834.1"
                     /translation="MKYKYTKYIFLVFCFCLSGTTTFAAKTLSLRLVRVEITNNNNNC
                     DEQCAWWTDKKMDWTFDVDDGSAGGWQTDVNEACQQMPGDNNDPGDWNANYIVWSNSY
                     PFVCSWPSSSVGVDFRLEGWDRDCDLCVGGGFANVGGDHVCTVTVNPTFPSSAFSGTG
                     GNLTYTCNFNSAYNGGSNCVGTITVVVRWEASGSFDAQTTDDYICGAFNMGTLGTGAT
                     LSRNNFSNIGYSTEDICAANEPNNDSNDETVWLKYKTGPNPGTAIEVDVDAIGGSGSG
                     GAFCISGGTVFAWTRVYEGPDDVGCPLTYSTNAFSQLSQVGSLGSLGTDNSLIDIDCP
                     KPNQTYYIQVEMQGIATCDMGRYNITVRDNGIQAGPNYICQPANSATIGVNGWMGTLN
                     SAFDPRTTTGSATAWENSDYDLRINNQSTACANVSSGEPDNTTVVSALNNTVWYRFRT
                     PAADYPTAPSLAGLAHTYRVFVERLGSSSTLTYPAVYLYEETAANARACGDNSTDYSN
                     LAFIDRDEIDPLNIFNGGNAELKRLCLKPNTNYFIQVDPVGGVGNSHVDFNLWVVKDQ
                     FRPSDMICDAVDLGYITTNGLQNGVTTGVNWANNGPASKFGAAKFFGLPHTNKCTSGE
                     AGEPNAAPGGPNPGSGTHSATVWYKFTTGSNIPPDWIYWYNDDNTLNNANRGSVCIGT
                     LFQSKVTFYKNATNFACPVGGNLVQGSEMNIPGDLCDATTGIGFGAPCYQDLFRLKCP
                     EPNTTYYVQVEDASISVCYEGQWTFDNGSHKITTASTLGGAPINDTICGAINLGTVPN
                     GGQINNGVIYDNFCATPDLPWRADFTQPLDADVWFRFRPPASGSVRITAQSAPSGSPS
                     IDDDLDIQAAIWEPILGDGTNAHCSDPRFLWTPIVAQDHGVCELSEQQTGFTNCFLGG
                     FFPYDIYNTCGNSAVCNENNSLIATCLDPNKYYYLQIDGGSYASCDLFDAGDCVMGYF
                     RLQVADAGLGLYNSASPGDVGTPFTSLGVPNSNLKHDEPCFAQSLPMNAAATSYGSLN
                     WTNMTNRCATSINDPVPSQWLSHNSSTDKTVWARFVAPPSGKVKIRAENIGQLKGDGD
                     YHDDINLQLALYQTSNCFDKWRLTEVGNGNGYDGGAVEIDILNDDDYTGVCVPCTGTC
                     GFEENYVGRCLIPGQTYYIMIDGDAGYVCAADVEDVEGDFRISVQELNGIPASTNDSI
                     CDAFLMTPISNSVGAVYNSPIAFNNECASIDQSYESTGKIADEINGTLFDFDVEHTLW
                     FEFVAPSTGKVKIEAIEDGGIDDIDLGIAIYDIPSQNCANIQTGGFKFEEDYDPAVFG
                     IASSDDEEITVSCLVPGRKYWVQVDGTSNMTTCGPAFSGSDCETGQFRMRVTTLAADQ
                     TYASNVVYSSPATTRPGGNDMFCQAHNVANAGGVYNPFNPNTYLGLNETITFTHNNRC
                     ATSELNEPEANGWDLNPFDEDSDPTVWYTFNTGPSVGPFGPGDITIRVTNPSGTCFDP
                     DVDLYEYNGTFTTANCNAQASTNTQFNNLIRIGEGKVFNVLRPREEEIKITCPKPNKT
                     YFLRILGSSTCPLFGSDQGDFDVSISEGSVGINFQTNDNICGATNVGSGILTSGGTLS
                     LPLQNNVCATQEVGEPNTSQSCVQNEPCYDETMWHKFTTGPNPGAVEVEITSLIGNGG
                     FFTVPSVAVYKYVPSGNPCSATPFANIVALDDDLGFATLLGGIIDPTAKVTLPCVSPN
                     TTYYIQVDGADVDLFGFTFPGFTDNYYYNVDVRDLGSGTGRASNDNLVNALPVDNVAP
                     LDGQLTVGGTLTVNGHNRCATCENGEIGDYCGNNTTDHTALLNAEDETVWYYFTTPNK
                     PGNINIRVEDDPGIAGTFSPNFRLYYNNGASPLYRTTTAPTASIVQEGSSYSGISSTN
                     GNFLCLLPNQRYYIQVDGNDNMPATLDQADFIVTVTDDGSGNPGPSNDLICNAENLTM
                     PTGILGRTNRCAWEETDEPNTSDNMGDSGDDVTSNNYDETVWFTFTVPSTQDVTMVLE
                     PTSGIAGGINYVLYEKTGTANISCSGSPADIPNWSQLREIASATGLVLNTIGTSDQVT
                     ATWPCLDPAKRYYIQVDGNDLLGSNDVGNFNIQLSSAVHANQVNDDICGIGATVTTGN
                     FGTFTVSATQTATTQDNRCATQEVGEPNINGPFNDITDPGYDHTLWYKFTASNTDGTY
                     TVSVNNVGGDAINASIVVYRQDAAVCPATNFSNITEVKASPATGSVTSDESLTLECWE
                     IEEGATYFVQIQGWDGIGGGDVGNNFNVSVQFNSGVTNPADNICTAPTVTVGAANTAA
                     DNRCATTQSGEPNIAPSPQSPTDGSNYDETLWYKFVAPASGEVRVNTSSYSPLALTLN
                     ADLYSIPATYNCAVDGFNGLIRDDNTSTLTTISPSWDLRCLVPGNTYYLRFDGNDGAL
                     GIPPDRGTWNFNISNLDNVTTYPTVPNDEPCGAIDVTQFVRTTPCTADGQYYTNAYSI
                     LDAAIDIDRATRSVQAIGCGGEANCNDYWFKFDVPLDATGIRIQGNDEYPIGT"
     gene            complement(7603..8112)
                     /locus_tag="PLL59_08235"
     CDS             complement(7603..8112)
                     /locus_tag="PLL59_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23835.1"
                     /translation="MKKIILFTFFITILSFLQAQNSVANVNVETVKLNNGFYQKYQYD
                     SSSECNCSEKTERDFKLAERLLKNDILDYNIISYNIDLGKNLNAIQKLFYAIKQDSTI
                     IKLSIIEWSKLSIVFTDGFDENSFFNAATQVFAKIEKSNVSEFLWQKNKELKLVYEDK
                     IRNLQSVNK"
     gene            complement(8168..9844)
                     /locus_tag="PLL59_08240"
     CDS             complement(8168..9844)
                     /locus_tag="PLL59_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23836.1"
                     /translation="MTVANDWECSFDSVYALGSPITLDVNDDAIKDIIISIGNQDTSN
                     RFVSGVVAINGDNGSLIWRNRRKQDVFGTGKLLDVNNDNFPEIISVGRSGELFALNAK
                     NGFLIWEFFDTGSLNSEDSGIYNFYDPQIINDVNNDNIKDILITNGGNPNAESNDTLN
                     RYQGYLLIVNGATGKVLAKLNSPDRRETYMAPIIHDFKNASDPVIIFGTGGETINGSL
                     WSIKLSDLLSEDSTKFIKLISDSTKGFITTPSLVDINNDGFLDIIAVSFNSNIYAIDG
                     ANFEIIWTKSFPNYESYTFPAVGLLNSDATPDIALSIGLGVFSSYTSTLHLVLDGVNG
                     NVLDSFSVNADFSLSSPIVFNIDADLYDEVLFFSSTAFSGIEKCNMHYVNPTKNTNQI
                     YDSIVDRFLFASTPLIASLNDDNYMDLIFASTQNFMDHNTKLLNTIIHKKKIANCQLY
                     EPAWGSIYGRNYDAKYDKNNNIPLAINNTSIAPVILNFNVSYANGFVHLNQLFDENEN
                     ATVQLLNQVGQLVLETKLKRQIYIGSLTNAIYTILVHTKDKLYVSRFVKL"
     gene            complement(9976..10503)
                     /locus_tag="PLL59_08245"
     CDS             complement(9976..10503)
                     /locus_tag="PLL59_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HMY23837.1"
                     /translation="MIIPKKVEKTLNVHLELEALAFFSYLAMASWCEKEGLEGCAKFF
                     RKQSDEEHVHFMKFFDYINEVGGHAEVPPVKQPKIDFKDILEVCQQSLLNEQKVTKSI
                     YNILKVTQQEDDHATEDFLQFFVMEQREEEVQFKRIIDKIHLIGKGPQSLYYIDKEFE
                     KLVSAGNKDEKKAKG"
     gene            10685..11650
                     /locus_tag="PLL59_08250"
     CDS             10685..11650
                     /locus_tag="PLL59_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMY23838.1"
                     /translation="MWKIDLKDTIEMQSTYERLYDKKAILQKARPLPKMALQKIKHDL
                     SIEWTYHSNSIEGNTLTLQETHLILKEGITVKGKSLREHFEAHNHEKAINYLYSLVED
                     NYVLRSIDILSLHALVLRSIEDDFAGRIRNGGVRIVGANFVPPSANKVSALLDELISF
                     VNTNPLGLNDIELATVFHHKLVWIHPFFDGNGRTVRLAMNLLLMRKGFPPAIILKNDR
                     KKYYEALNQANKGSYQKLTLLMAQALERSLNIYLHALPNTDEGDEYKEISMLVEEPNF
                     PYNQEYISLLARQGKIDAYKEGRNWLTTKNAIENYIKNRKRERIL"
     gene            complement(11878..>12873)
                     /locus_tag="PLL59_08255"
     CDS             complement(11878..>12873)
                     /locus_tag="PLL59_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HMY23839.1"
                     /translation="EGLIKHVFKTVKGIELDDFPRMQYDDAMKLYGSDKPDIRFGMQF
                     VEMNELVKGKGFGVFDNAELVIGINAENAAEYTRKQLDELTDFVKRPQIGATGLIYLR
                     YNNDGTLKSSVDKFYNEEELKKWAAAANMKPGDLLLVLCGNIDKVRKQMNELRLELGN
                     RLGLRKKDDFKPLWVLDFPLLEFIEEDNRWFAMHHPFTSPKKEDIEFLYTDPARVRAD
                     AYDLAINGVEVGGGSIRIFDKTLQAEMFKRLGFTDEEAQKQFGFLMNAFEYGAPPHGG
                     LAFGFDRLCAVLNSVETIRDFIAFPKNNSGRDMMLDSPSTIDEKQMKELNIKTTL"
BASE COUNT         4009 a         2534 c         2217 g         4113 t
ORIGIN      
        1 gttccgattg gatattcatc attaccttga atacggatac ctgtcgcatc taatggcaca
       61 tcaaacttga accagtagtc attacagtta gcttcgccac cgcatccaat tgcttgaaca
      121 gaacgtgttg ctctatctat atctattgca gcatcaagaa tactataagc attggtataa
      181 tattgaccat ctgcagtaca aggtgttgtt ctgacaaact gtgttacatc gattgcacca
      241 caaggttcat cgttcggcac tgttggatat gtagtaacat tgtctaagtt agaaatatta
      301 aaattccatg tgcctctgtc tggcggtatg cctaatgcac catcgttgcc atcaaatctt
      361 aaataatatg tgtttcctgg cacaaggcat cttaaatccc aacttggtga aatcgttgtc
      421 aaggtgcttg tattgtcatc tcttatcaaa ccattaaagc catcaactgc acaattatag
      481 gttgctggaa tgctatataa atcagcattt agtgtcaatg ccaaaggact ataagaagaa
      541 gtatttacac gcacttcacc tgatgctggt gccacaaatt tgtaccataa tgtttcatca
      601 taattcgaac catctgttgg tgattgtgga gatggtgcaa tatttggctc gccactttgt
      661 gttgtagcac atctattgtc tgctgctgta tttgctgctc ctactgtcac tgttggtgct
      721 gtacaaatat tatctgccgg attggtaaca ccactattga actgaacaga cacattgaaa
      781 ttatttccta catcgccgcc accgataccg tcccagcctt gtatctgaac aaaataggtc
      841 gctccttctt caatttccca acactctaat gttagcgatt catcgcttgt aacactacct
      901 gttgctggag atgcttttac ctctgttata ttagagaaat ttgtagcagg acaaacagca
      961 gcatcttgac gataaacaac aattgaagca ttgattgcat cgccaccaac attatttaca
     1021 gatacggtat atgtaccatc tgtattactt gctgtaaatt tataccaaag tgtgtggtca
     1081 tatccaggat cagtaatatc gttgaaagga ccgttaatat taggttcgcc gacctcttgt
     1141 gttgcacatc gattatcttg tgttgttgca gtttgtgtag cagaaaccgt aaatgttcca
     1201 aaattaccag tagtcactgt agcaccaatt ccacatatat catcattcac ttgatttgca
     1261 tgaacagctg aacttagttg gatattaaaa ttgcctacat cattggagcc taacaaatca
     1321 ttgccatcta cttgtatgta ataacgtttt gcagggtcaa ggcaaggcca cgtagctgta
     1381 acttggtccg atgttccgat ggtattgagc acaagacccg tagcagatgc aatttctctc
     1441 aattgcgacc aattcggtat gtcggctggt gagcctgaac aagaaatgtt tgcggtacct
     1501 gttttttcat ataatacata atttattcca ccagcaattc cagatgttgg ttctaatacc
     1561 attgttacat cttgtgtaga tggaacggtg aatgtgaacc aaacggtttc atcgtaatta
     1621 ttagaagtaa catcatctcc actgtcgccc atattgtctg aggtatttgg ctcatctgtt
     1681 tcttcccatg cacatctatt cgttcgacct aagatacctg ttggcatggt caagttttca
     1741 gcattacaaa ttaaatcatt acttggccct ggattacctg aaccatcgtc agtaacggta
     1801 acaataaagt ctgcttggtc taaagttgct ggcatattat catttccatc aacttggatg
     1861 taataacgct gatttggtaa taagcataag aaatttccat tagtcgaact tatgccacta
     1921 taagaagagc cttcttgaac tatgcttgca gttggcgctg ttgttgttct gtataatggt
     1981 gatgcaccat tgttataata taatctaaaa ttagggctga atgtaccagc aattcctgga
     2041 tcatcttcta cgcgtatgtt gatatttcca ggtttattag gtgttgtaaa ataataccaa
     2101 actgtttcat cttctgcatt taataacgca gtatgatctg ttgtattatt gccacaataa
     2161 tcgccgattt caccattttc acatgttgcg caacgattat gaccatttac tgttaaagta
     2221 ccgccaacag taagttgccc atctaatggc gcaacattat ctactggcaa agcattgact
     2281 aaattatcat tagaagctct ccctgtaccg ctacctaaat ctcgaacatc gacattgtaa
     2341 tagtagttat ctgtaaagcc tggaaaagta aatccaaata aatccacatc agcaccatca
     2401 acttgtatat agtaggtagt atttggagaa acacaaggca aggtgacttt tgcagtagga
     2461 tctataattc cgcctaataa agttgcaaat cctaaatcat catctaaagc tacaatatta
     2521 gcgaatggtg tcgctgaaca aggattgcca gatggcacat atttatatac tgcaactgaa
     2581 ggcacagtga agaaacctcc atttccaatc aatgaagtta tctctacttc tactgcacca
     2641 ggatttggac ctgttgtaaa tttatgccac atggtttcat cgtagcaagg ttcattttga
     2701 acacaacttt gcgatgtgtt aggctcacca acttcttgtg tagcacacac attattttga
     2761 agcggaagag acagtgtgcc tccacttgtt aatatgccgc tacctacatt tgtagcacca
     2821 caaatattat cgttggtttg gaaattgata cctacactac cttcagaaat ggacacatca
     2881 aaatcacctt ggtcggagcc aaataatgga caagtgctac ttcctaagat gcgcaagaaa
     2941 tatgttttgt tcggttttgg acaagttatt tttatttctt cttcacgtgg tctaagcaca
     3001 ttgaaaactt taccttcgcc gatgcgaatt aaattattaa actgtgtgtt ggtgctagct
     3061 tgtgcattac aatttgctgt tgtgaatgtt ccattgtatt catacaaatc aacatcaggg
     3121 tcaaagcaag tgccagatgg attagtaaca cgaatggtga tatcgcctgg accaaatgga
     3181 cctacacttg ggccagtatt aaaagtgtac cataccgtag gatcgctatc ttcatcaaaa
     3241 ggattaagat cccaaccatt tgcttctggt tcattcaatt cagaagtagc acatctatta
     3301 ttatgtgtga atgttatcgt ttcgtttaat cctaaatagg tgtttggatt aaatggattg
     3361 tacacaccac cagcgttagc gacattgtgt gcttgacaga acatatcgtt accgccaggc
     3421 cgtgttgtag caggcgatga ataaactaca ttagatgcat aagtttggtc agctgccaat
     3481 gttgtaacac gcattctaaa ttgacctgtt tcacagtctg aacctgagaa agcaggaccg
     3541 cacgtagtca tattgcttgt tccatcaact tgcacccaat attttctacc tggcactaag
     3601 caagaaacgg taatttcttc atcatcagaa ctagcaatac caaatactgc tgggtcataa
     3661 tcttcttcaa atttaaaacc gccagtttgg atatttgcac aattttgaga tggaatatca
     3721 tagatggcaa tgcctaaatc aatatcatcg attccgccat cttcaatagc ttctattttt
     3781 acttttcctg tcgatggcgc cacaaattca aaccatagcg tatgttctac atcgaagtcg
     3841 aataatgtac catttatttc gtcagcaatc ttaccagtac tttcataaga ttggtctatg
     3901 cttgcacatt cattattaaa tgcgattggt gaattataaa cagcacctac actatttgaa
     3961 attggtgtca tcaagaatgc atcacaaata gaatcattag tagatgcagg aatgccattt
     4021 agttcttgaa cggaaattct aaaatcacct tcgacatctt ccacgtcagc agcacataca
     4081 tatccagcat caccgtctat cataatataa tatgtttgtc caggaataag acatctacct
     4141 acataatttt cttcaaatcc gcaggttccg gtacacggca cacatacacc tgtataatca
     4201 tcatcattca gaatatcgat ttcaacggca ccgccatcgt aaccatttcc atttcctact
     4261 tctgttaatc tccatttgtc aaagcaatta gaagtttggt agagtgccaa ttgcaagttg
     4321 atatcatcat gatagtcacc atcacctttt aactgtccaa tattttctgc tctaattttc
     4381 actttaccgc ttggtggtgc tacgaagcgc gcccaaaccg ttttatctgt tgatgaatta
     4441 tgagaaagcc attgtgaagg tactggatca ttaattgaag tggcacatct attggtcatg
     4501 tttgtccaat tcaatgagcc atatgaagtt gcagcagcat tcattggcaa actttgtgca
     4561 aaacaaggtt catcgtgttt taagttagaa ttcggaacac ctaaagaagt aaaaggcgta
     4621 cccacatctc ctgggcttgc actattatat aagcctaagc cagcatcagc tacttgcaat
     4681 cgaaagtaac ccattacaca atcgccggca tcaaataaat cgcaagaagc ataactaccg
     4741 ccatcaattt gcaaataata atatttatta ggatcgagac atgtggctat taagctatta
     4801 ttttcattac acactgcgct attaccacaa gtattataaa tatcatatgg gaaaaaacca
     4861 cctaagaaac agttggtaaa acctgtttgt tgttctgaca attcacaaac gccgtggtct
     4921 tgtgcaacaa ttggcgtcca taaaaatcta ggatcagagc aatgtgcatt ggtgccatca
     4981 cctaaaattg gctcccaaat tgctgcttgg atatctaggt cgtcgtctat gcttggtgag
     5041 ccagatggag cagactgtgc tgtaatccga acagaaccag aagcaggcgg tctaaaacga
     5101 aaccatacat cagcatctaa tggttgagtg aaatctgcac gccatggtaa atcaggcgtt
     5161 gcacagaaat tgtcatagat gacgccatta tttatttggc ctccatttgg tactgtacct
     5221 aaatttattg caccacaaat tgtatcattt ataggagcac cacctaaggt agatgcagtt
     5281 gtgattttat ggctgccatt atcaaatgtc cattgacctt cataacacac ggaaatcgaa
     5341 gcatcttcaa cttgcacata ataggttgta tttggctctg gacattttaa gcggaataaa
     5401 tcttgatagc acggtgcacc gaagccaatg ccagtagtag cgtcacacag atcacctgga
     5461 atattcattt cagaaccttg tactaaattg ccacccactg gacaagcaaa gtttgtcgca
     5521 tttttataaa aggtaacttt actttggaat aaggtaccaa tacaaacact acctctattc
     5581 gcattattca gtgtattatc atcattatac caataaatcc aatctggtgg aatgttacta
     5641 cctgtagtaa acttatacca gactgttgca gagtgcgtgc cactaccagg gtttggtccg
     5701 cctggagcgg cgtttggttc gcctgcttct ccggaagtac atttattagt atgtggtaat
     5761 ccaaaaaact tagctgcacc aaatttagat gctggtccgt tattcgccca attcacgcct
     5821 gtggtcacgc cattttgcaa gccgtttgtc gtaatatatc ctaaatcaac tgcatcacaa
     5881 atcatatcag aaggtcggaa ttggtctttc acgacccata aattaaaatc gacatgtgaa
     5941 ttgcctacac cacccactgg gtcaacttga ataaaataat ttgtattcgg ttttaggcat
     6001 aatcgtttta attccgcatt tccaccattg aatatattta atggatcaat ttcatctcta
     6061 tcgataaatg ctagatttga ataatcggta ctattatcgc cacaagcacg agcatttgct
     6121 gcagtttctt cgtataaata aacagctgga tatgtcaaag tacttgatga gcctaaacgt
     6181 tctacaaaaa cgcgataggt atgtgccagc cctgctaaac ttggtgctgt tggataatct
     6241 gctgctggtg ttctaaatcg gtaccatacc gtgttattca atgcagacac gacagtagta
     6301 ttatcaggtt caccactact tacattcgca catgccgttg attggttatt gatacgtaaa
     6361 tcgtaatctg agttttccca agctgttgcg gagcctgttg ttgttctagg atcaaatgca
     6421 ctattaagtg ttcccatcca cccattaaca ccaatagtgg cagagttagc aggttggcaa
     6481 atataatttg gtccggcttg aatgccatta tctcttacgg tgatattata acgtcccata
     6541 tcgcaagtag caataccttg catttctact tggatatagt aagtttggtt tggtttagga
     6601 caatcgatgt ctatcaaact attgtctgtg ccaagagaac ctaaagagcc gacttgcgaa
     6661 agttgtgaaa atgcattagt agaataagta agtggacatc ccacatcatc gggcccttca
     6721 taaactcttg tccaagcaaa tacagtacca cctgaaatac aaaatgctcc tcctgaaccc
     6781 gagccaccga tggcatctac atcaacttct attgctgtgc caggattagg acctgtcttg
     6841 tatttaagcc aaacggtttc gtcatttgag tcgttattgg gttcgtttgc tgcgcaaata
     6901 tcctctgtgc tataacctat atttgagaaa ttgttgcgag ataatgttgc tcctgtacct
     6961 aaggttccca tattaaacgc accgcagata taatcgtcag tggtctgcgc atcaaaagag
     7021 ccagaagctt cccaacgtac aacaacagtt attgtaccaa cacaattcga tccaccattg
     7081 taagctgaat taaaattaca agtgtaggtt agattcccac ctgtaccaga aaatgcactc
     7141 gaagggaaag tcggatttac ggtgaccgta caaacatgat cgccgccaac atttgcaaat
     7201 ccaccaccaa cacacaaatc acaatctcta tcccaccctt caagtctaaa atcgacacca
     7261 acactcgaag atggccaact acaaacaaat ggatacgaat ttgaccaaac aatgtagttt
     7321 gcattccaat caccagggtc gttattatcc cctggcattt gttggcacgc ttcgtttaca
     7381 tcagtttgcc aaccaccagc acttccatca tcaacatcaa atgtccaatc cattttttta
     7441 tccgtccacc atgcacattg ttcatcacaa ttgttgttgt tatttgttat ttctactcga
     7501 actaatctaa gagataatgt cttagcagca aaagtagtgg tacctgataa acaaaaacag
     7561 aaaacaagaa aaatgtattt tgtatattta tatttcatat attcatttat ttacagattg
     7621 taagttgcgg attttatctt catacaccaa tttcaattct ttattttttt gccataggaa
     7681 ttcgctaaca tttgattttt ctatttttgc aaaaacttga gttgcggcat taaaaaagga
     7741 attttcatca aatccgtctg taaagacaat agataatttt gaccattcaa taatagataa
     7801 tttgataatt gtggagtctt gttttattgc ataaaatagt ttctgaatag catttaaatt
     7861 tttgcctaaa tctatgttgt aagaaataat gttgtagtct agaatatcat ttttcagcaa
     7921 acgttctgca agcttaaaat ctctttctgt tttttctgag caattgcatt cagaacttga
     7981 atcgtattga tatttttgat agaagccatt gttcagtttt acggtctcaa catttacatt
     8041 tgcaacagaa ttttgtgcct gtaaaaatga aagtatagta ataaaaaaag taaaaagtat
     8101 aattttcttc ataacattga aattgaatgt caagttaata ataaattagt cattttgcaa
     8161 tataaatcta taatttaaca aatctagata cgtacaattt atctttagta tgcaccaaaa
     8221 tcgtatatat cgcattagtt aaggatccga tataaatctg ccgttttaat ttggtttcca
     8281 ataccaattg tcctacttga tttaacagtt gcacggtagc attttcattt tcatcaaaca
     8341 actggtttaa gtgaacaaaa ccatttgcat atgaaacatt aaaatttaaa ataacaggtg
     8401 ctatagatgt attatttata gctaaaggta tattgttatt cttatcatac ttggcgtcat
     8461 aatttctgcc ataaatgctt ccccaggctg gttcataaag ttgacagtta gctatctttt
     8521 ttttatgtat aatagtgttt agtaatttgg tgttatggtc cataaaattt tgagtagatg
     8581 caaagattaa atccatgtaa ttgtcatcat taagggaggc gattaatggt gtagatgcaa
     8641 atagaaatct atccactatt gaatcataaa tttgatttgt attttttgtt ggatttacgt
     8701 aatgcatatt acacttctca attcctgaaa atgcagtact agaaaaaaat aaaacttcgt
     8761 cgtaaaggtc agcatcaata ttgaagacaa taggcgaaga aagtgaaaaa tctgcattta
     8821 cagaaaacga gtccaagaca tttccattta ctccatcaag caccagatgt aatgtagatg
     8881 tatacgatga aaaaacacct aagccaatac tgagggcaat gtctggtgtt gcatctgaat
     8941 tgagtaagcc gacagctgga aatgtataag attcataatt aggaaatgac ttagtccata
     9001 taatttcaaa attagcacca tcgatagcat atatgttgct attaaaactt acggcaatga
     9061 tgtcaagaaa accatcattg tttatgtcga caagcgaagg tgtcgttata aatccttttg
     9121 ttgaatctga aatgagttta ataaattttg tagaatcttc tgatagcaag tcgctcagtt
     9181 taatagacca taagctacca tttatcgttt cgccaccagt accaaagatt ataacagggt
     9241 ctgaggcgtt tttaaaatca tgaattattg gtgccatata agtttctctt ctatcaggag
     9301 aattgagctt tgctaatact ttacctgttg caccattaac aattaataag tatccttgat
     9361 aacgatttaa agtatcattt gattcagcgt ttggattacc tccgttggta ataaggatat
     9421 ccttgatatt gtcattattt acatcattaa ttatctgtgg gtcgtaaaaa ttataaatgc
     9481 ctgagtcttc tgaatttaaa cttcctgtat caaaaaactc ccaaataaga aaaccatttt
     9541 ttgcattaag tgcaaataac tctccgcttc tacctacaga aataatttca ggaaaattat
     9601 cattattgac atctaagagt ttacctgtac caaacacatc ttgctttcgt ctatttcgcc
     9661 agattagact gccattatct ccatttattg caacaacacc agatacaaag cggttgctcg
     9721 tatcctggtt acctattgaa atgattatat cttttattgc atcatcgtta acatctagtg
     9781 taattggtga gcctagcgca taaacgctgt caaaactgca ttcccaatca tttgcgacgg
     9841 tcactttact tagaaccata cagataaaaa cgaggaatgt gcttatcttt aatgatttca
     9901 tattaatagg aaacggattt atcggctact acataataat ttctataaca ctctattcaa
     9961 ctagaaagaa ttcaactatc ctttagcttt tttttcgtct ttatttcctg cacttactaa
    10021 tttttcgaat tctttgtcga tgtaatataa gctttgaggt cctttgccta ttaagtgaat
    10081 tttgtcgatg atgcgtttaa actgcacttc ttcttcgcgt tgttccatca cgaaaaactg
    10141 taggaaatct tctgttgcgt gatcatcttc ttgctgtgtg acttttaaaa tattatagat
    10201 agacttggtt actttttgtt cattaagtag cgactgttgg caaacttcta aaatatcttt
    10261 aaagtcgatt ttaggttgct ttacaggagg cacttctgca tgtccgccta cttcattgat
    10321 ataatcaaaa aatttcatga agtgtacatg ctcttcatcg gattgttttc tgaaaaattt
    10381 agcacaacct tctagtcctt ctttttcaca ccacgatgcc attgccaaat aactaaagaa
    10441 tgccagtgct tctagttcta aatgtacatt taatgttttt tctactttct taggaataat
    10501 cataacgctg aattgttctg caatttacaa ctaataaaac aaaattgtaa acaaaaagat
    10561 attctataaa tagaacaaaa accagcttcg aattacatgc attacttaat aaaatcaaaa
    10621 ataaaattaa tatcggataa gaaaagtttg caacttcccg atatttgcaa taactttgtg
    10681 atagatgtgg aagatagatt taaaagatac catagaaatg caatcaacat acgaaagatt
    10741 gtatgataaa aaagcaatat tgcaaaaagc aagaccattg cctaaaatgg ctttgcaaaa
    10801 aataaaacat gacttaagta tagaatggac ttatcattca aacagtatag aaggaaatac
    10861 gcttacactt caggaaacac atttaatttt aaaagaaggt attaccgtaa aaggtaaatc
    10921 attaagagag cattttgaag cacataacca cgaaaaagcc attaattatt tgtattcatt
    10981 agtcgaggat aattatgtat taagaagcat tgatatctta tcgttacatg cgttagtatt
    11041 gcgcagtatc gaagatgatt ttgccggaag aatacgcaat ggcggcgtgc gtattgttgg
    11101 tgctaatttt gttccgccaa gtgccaataa agtttctgcc ttgttagatg agctaatttc
    11161 gtttgtcaac accaatcctt taggattgaa tgatattgaa ttggctacag ttttccatca
    11221 caagttggta tggattcatc cgttttttga tggcaacggt agaacagtgc gtttagcaat
    11281 gaatttatta ctgatgcgta aaggttttcc gccagccatt attttaaaaa atgacagaaa
    11341 gaaatattac gaagcactca atcaggcgaa taaaggtagc tatcaaaagc ttacgttgct
    11401 gatggcacag gcattagaac gttctttgaa tatttactta catgctttgc caaatacaga
    11461 tgaaggtgat gaatacaaag aaatttctat gttagttgaa gagccgaatt ttccttacaa
    11521 tcaagagtat attagtttgt tggcgcgaca aggaaaaata gatgcttaca aagaaggtag
    11581 aaactggctc accacaaaaa atgcgattga aaattacatt aagaacagga agagagaaag
    11641 gatattgtag aaaacacaga atccttttca agactctact gagaaagtaa actggttcac
    11701 taccaaaaaa gctattgaag attacataag gtaatgtggt caaaaatgga gcttttaatt
    11761 acatctagta atgatttttt gtatggaata ttttaagaat gctttttagt ttcttcaatt
    11821 ttatttagat tatcattgta tttttctgaa ttactcattt ataaatacta aaatagctta
    11881 caaagtcgtc ttaatattca actctttcat ctgcttttca tctatcgtgc ttggcgaatc
    11941 tagcatcata tcgcgaccag aattattttt tggaaatgca atgaaatcac gaatggtttc
    12001 tacactattt aaaacagcgc ataatctatc aaaaccaaat gctaagccgc cgtgtggtgg
    12061 tgcaccatat tcaaaagcat tcatcaaaaa tccgaattgt ttttgcgctt cttcatctgt
    12121 aaaacctaag cgtttgaaca tttctgcctg taatgtttta tcaaaaatac gaatagaacc
    12181 accgccaact tcaacgccgt taatagctaa atcgtacgca tcagcacgca cgcgcgctgg
    12241 gtcggtatat aaaaattcta tatcttcttt tttaggtgat gtgaatggat gatgcatcgc
    12301 aaaccaacga ttatcttctt cgataaattc caacaatgga aaatctaaaa cccacaatgg
    12361 tttgaaatcg tcttttttgc gcaagcctaa acgattgcct aattctaatc gcaattcatt
    12421 catttgtttg cgcactttat ctatattgcc acataacacc aacagtaaat cgcctggttt
    12481 catattggca gcagcagccc atttcttcaa ttcctcttca ttataaaatt tatctacaga
    12541 agattttaag gtaccgtcgt tgttgtatct taagtaaatc aatccggtgg cgccaatttg
    12601 tggacgcttc acaaaatccg tcagttcatc cagttgcttg cgtgtatatt cagcagcgtt
    12661 ttctgcatta ataccaatca ccagttccgc attgtcaaat acaccaaagc cttttccttt
    12721 caccaactca ttcatttcta caaactgcat accgaagcga atatccggct tgtcgctgcc
    12781 gtataatttc atcgcatcgt cgtattgcat gcgcgggaag tcatccagtt caatgccttt
    12841 caccgtttta aaaacatgct taatcaagcc ttc
//
LOCUS       DAOQQV010000072        12858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1669_length_12858_cov_7.431227, whole genome shotgun sequence.
ACCESSION   DAOQQV010000072 DAOQQV010000000
VERSION     DAOQQV010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12858
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1669_length_12858_cov_7.431227"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..610
                     /locus_tag="PLL59_08260"
     CDS             143..610
                     /locus_tag="PLL59_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23840.1"
                     /translation="MFQEIYNDIQAKMQQQIQEKFGLSADQTTQSTNVLLDNFKKFFS
                     EDLMAGGMAGLKDIMSNGISDIKNNPTMQTLRENIVNDLVNKVGLAEETAQKVRDFSL
                     TEVFDKLKTEFLDENGKPDFSKIMSKINMADFQGKAQEMMGKMGMDFGKLFGK"
     gene            complement(619..1686)
                     /locus_tag="PLL59_08265"
     CDS             complement(619..1686)
                     /locus_tag="PLL59_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HMY23841.1"
                     /translation="MTKIQDDAQIQFIELLQKLIGVSSFSKEEDKAADIVRKFLKSKK
                     IPFDTKLHNTWAKNKHYNFLKPTILLNSHIDTVKPVNGWVKDPFSAEVQDDKLYGLGS
                     NDAGAALISLLATFLHFYDKEDLKYNLIFAATSEEEISGKNGIEQIEDITSSCEFAIV
                     GEPTEMKMAIAEKGLMVFDGEVKGKAGHAARNEGVNAIYLAMTDVQWFQNYQYKKINP
                     VLGPVKMTVSMIQAGTQHNVIPDVCKYTVDVRTIPEYTSEQIIEIIKDNVKADIKVRS
                     TRLQPSQIDKEHLIVKAAEKLGIETFGSSTLSDQTLLKIPSIKMGPGKSERSHTADEF
                     IYLQEIEDGLKIYRKLLEQVL"
     gene            complement(1688..2479)
                     /gene="argB"
                     /locus_tag="PLL59_08270"
     CDS             complement(1688..2479)
                     /gene="argB"
                     /locus_tag="PLL59_08270"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HMY23842.1"
                     /translation="MERILVIKVGGNIIDKEEALHQFLQDLSKINTPKILIHGGGKIA
                     TQLSKQLGIESKLVDGRRITDEKTIDVVTMVYAGLINKKIVAQLQANNCNAIGICGAD
                     ANSILSHKRVNNLIDYGFVGDIDEVNMDFFLSLLEKQLTPIIAPITHDKNGLLLNTNA
                     DSLAAAIAIALSKDKKVSFYYCFEKNGLLENVNNDDSIISHVKSSEIEQLKEKQIITD
                     GMLPKIDNIKYALENGVEKVILCHAQNILSNVNENAKFGTTFTKN"
     gene            2511..3494
                     /locus_tag="PLL59_08275"
     CDS             2511..3494
                     /locus_tag="PLL59_08275"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HMY23843.1"
                     /translation="MNKNLLIVYVILFSILSCQKNTPQKLYSKIVTAPGPEDIVLDKA
                     NNRILVSCDERRTGMALQAAIMQIDLNTDICTALPIVNMPSIPFHPHGFDLQTVNGID
                     YLYIINHYRDSAYTNAVLQFEIKASQLEFVREYKHPLLISPNDITVLPNGAFYFSNDK
                     NSSNILEMLYSPYGGSVVYCNGATTWQKVDSIIGFPNGLYDEGDALYLATSRNYAMYK
                     YEMQSDGTLKNRKRITAINGMDNIIENGDDLIVSVHPDEIKFALLSYFPQTLSPSKTY
                     AINKQTGQARLIFDDDGKKISGSSTALVHGNDLYLAQVFDGFVLKIANYKY"
     gene            3540..4559
                     /locus_tag="PLL59_08280"
     CDS             3540..4559
                     /locus_tag="PLL59_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23844.1"
                     /translation="MKHGITILIVFMYTLGFSIEKIPETPFTEYRFNAAECKLIQHHQ
                     SRIFIPSFAFYLDGKLYHGDVVLKYREFVDQLDLILNNIPMAYQENGKQHVLESAGMF
                     EFLAYGNGKLLSFAPAKKAQVQLATAFNMTGGETFILNRQTNTWRKDTPFASLSESNR
                     EIGDDSNALWPENIWNDAVNNDEIFDSSGNTNVAMSSSNSNTLSFETLSNQAFKTLNV
                     DKMEVYNCDKILTGETIPILADFNLQGFKQALHSPVYVVYKNRNAVLTYFPEQFKSDF
                     KLIANEDFTIFTFSMDGKIAVLDNAFAANFDAKNYRNKTVLFPLKVFAKAPNTKEELA
                     KITGL"
     gene            4580..5218
                     /locus_tag="PLL59_08285"
     CDS             4580..5218
                     /locus_tag="PLL59_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23845.1"
                     /translation="MRKYFLIFFMFSSVSSASAQHLFVADTFIYTINLDLSKNNYLKT
                     VPLDLLNGYCHGNWKAYYPKRTLNECLLDDFLQHFNVFQINTATFSCLEDYCANPYFK
                     DFFQQFTRKLKYSEIVFFDQQHSFVKREIISLQVFYSRNEYNGWKHYEGPIFWMQELK
                     KFPELIKVYNKNVRPDAWTLEKEFNHAGFITNENKQNDDKQKLQKILQIEEY"
     gene            complement(5215..5805)
                     /locus_tag="PLL59_08290"
     CDS             complement(5215..5805)
                     /locus_tag="PLL59_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23846.1"
                     /translation="MLRNLHALLTGAFLFIFTFLLANGNPKDVIRLGSKLIYDVNFEG
                     ASYEVTVIVKNNNENYTFEWFMSKPANGKGTIQLSKAAIENASGILSHFTNGDISLDD
                     QCCIFLSKKMFEELKNDGSMEIVTDKKKNVKTIFGNPYQHTQSYGYKNNYTNEFQCRT
                     VSDGGDYQITYVDDADFPLIVELNLDWSLKLKNIED"
     gene            complement(5889..6836)
                     /locus_tag="PLL59_08295"
     CDS             complement(5889..6836)
                     /locus_tag="PLL59_08295"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HMY23847.1"
                     /translation="MKNFLNIHDVVNAEQLAQEAIAIKQQKFANKSIGKDKTMVLLFL
                     NSSLRTRISTQKAAHNLGMQTIVLNLGQDSWQMEYEDGAVMNGTTAEHIKEGAAVISE
                     YADIIGIRAFPTLTDREKDYSEYVLHKFLENVNVPVVNMESATVHPLQSLTDLMTIRE
                     YSKKEKPKVVLSWAPHIKPLPQCVANSFAEWMNHADVEFVITHPKGYELNEKFSGNAH
                     IEYMQDKAFENADFIYAKNWSAYDSYGKILGDNPDWMITDKKMQLTNHANFMHCLPTR
                     RNLEIADDVLDSAYSLVIKQAANREYAAQAVLKKILENL"
     gene            complement(6833..7960)
                     /locus_tag="PLL59_08300"
     CDS             complement(6833..7960)
                     /locus_tag="PLL59_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMY23848.1"
                     /translation="MKLFDVYPLFNIQLERGKGCYVYDKNNTEYLDFYGGHAVISIGH
                     GHEHYIQKLNEQLHRLTFYSNSVQLEIQQQVATKLGKLSGYEDYHLFLINSGAEAVEN
                     ALKVASFYNNRKKVIAFRGAFHGRTSGAVAVTDNPKIKPPINDDAHVIFLDFNDEASL
                     ENAFKSNEICAVIIEPIQGVNGIYEPNAAFLQLIQKLCLEHGALFIADEIQCGFGRSG
                     QFFAHQYANVQPDIITMAKGMGNGFPIAGILIHPKIIAQYSMLGTTFGGAPLACAAVM
                     AVLDVIENEHLIDNAINMGNYLKEKCEQLPHVKEIRGKGLMIGVEFDFPIKEIRDKLV
                     YKHHIFVGNSNQPNTMRFLPSLTVTKKEIDQLINALNSLLK"
     gene            complement(7982..8962)
                     /gene="argC"
                     /locus_tag="PLL59_08305"
     CDS             complement(7982..8962)
                     /gene="argC"
                     /locus_tag="PLL59_08305"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HMY23849.1"
                     /translation="MEINNKIKVGIIGAAGYTGGELIRILLHHPNVNIEYCYSRSQHG
                     KKIYDVHTDLIGDIDLHFTNELSKEIDVAFLCLPHGESKHFIENNSFSPTTKIIDLSN
                     DFRLKKNANNFVYGLPELKKNEIKNAKHLANPGCFATAIQLALLPLAKENLLQNDIHI
                     SAVTGSTGAGVSLSETSHFTWRNNNFSVYKPFTHQHLGEIKESLHQLQESFDASIKFI
                     PYRGDFTRGILATIYVEFKDSLDAAKQLFINYYRDEPFVVISNKNIDLKQVVNTNKCL
                     LYLEKHENQLMIISMIDNLVKGASGQAVQNMNLMFALDETTGLKLKSVAY"
     gene            complement(9105..9404)
                     /locus_tag="PLL59_08310"
     CDS             complement(9105..9404)
                     /locus_tag="PLL59_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23850.1"
                     /translation="MEQTLTYNRKALITSFEKATSITNSVKIDTILEAKQIINHLIHY
                     LAINDINSIEDDFITPDFNKDTIKIRFTATNDKVTNQSFLSSIKIFNDYIFATNK"
     gene            complement(9519..10826)
                     /gene="ffh"
                     /locus_tag="PLL59_08315"
     CDS             complement(9519..10826)
                     /gene="ffh"
                     /locus_tag="PLL59_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HMY23851.1"
                     /translation="MFENLQDKLEGAFKNLKGQGKLTELNIAATVKDIRRALVDADVN
                     YKIAKEFTDTIKDKALGQNVLTAVSPGQLMVKIVSDELTQLMGGTASEINIKGSPAVL
                     LISGLQGSGKTTFSGKLAHYAKTKLNKTPLLVACDVYRPAAIDQLKVLGEQIGVEVYS
                     EIENKNPVAIAQNAIAYAKQKGQNFVIVDTAGRLAVDEEMMNEIYQVKQAIQPQETLF
                     VVDSMTGQDAVNTAKAFNDRINFDGVVLTKLDGDTRGGAALSIKYTVNKPIKFVSRGE
                     KLIDTLDIFYPERMSQRILGMGDIVSFVEKAQEQFDEKEAQRLQKKIAKNQFDFEDFL
                     QQINQIRKMGNIKDLLGMIPGVGKAVKDIDIDDNAFKKIEAMIFSMTLEERRNPDLLN
                     GKRRERIAKGSGSTIQEVNAFVKQFDQMKVMMKRMQGMGMGKR"
     gene            complement(10876..12300)
                     /locus_tag="PLL59_08320"
     CDS             complement(10876..12300)
                     /locus_tag="PLL59_08320"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMY23852.1"
                     /translation="MGNWNSKEKTALELINTAGLLWLERNIELVVIRRNIVDARYSEV
                     LNHKEYAKKYLGLPLTIELLNNIASAIAKLDLAPTRIDLATLAKEALEANVTDFDAFV
                     LEKLANYIGKDKRVIKPIDVVLFGFGRIGRLAARELIQQYGNGEQLVLKAIVTRGKNT
                     DEEIKKRSSLLRKDSVHGKFRGVITEDFDNKALVANGNKIHMIVADNPAEIDYTQYGI
                     KDALLIDNSGIFRDEEKLSTHLKAKGIAKVLLTAPGKGSLPNIVYGVNHLDFNPDATN
                     IFTAASCTTNAISPIIKVIADKLGIESGHVETVHSYTNDQNLLDNYHKSYRRGRSAPL
                     NMVITETGAASAITKVMPSMAGKFTGNAVRVPTPDVSLAILNLNLSTAVESIDAVNAI
                     IREAATFGNLVEQIDYSTSQELVSTDCVGNSHASILDSHATLISPNKKSIVLYVWYDN
                     EYGYTRQVIRLAKYLGKVQRLTYY"
     gene            complement(12379..>12858)
                     /locus_tag="PLL59_08325"
     CDS             complement(12379..>12858)
                     /locus_tag="PLL59_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMY23853.1"
                     /translation="KVGSLNKINKAFSNLEQEFEREPSADELAEMLEITREEVETTLG
                     VAARHVSVDAPFQEGEENSLLDVLENTSAPRTDLQLEYLESLRKEIERSLSTLTDRQK
                     DVIKYYFGIGVEHPMSLEDIGEKFGLTRERVRQIKDKAINKLRATSRSKLLKNYLGN"
BASE COUNT         3987 a         2128 c         2089 g         4654 t
ORIGIN      
        1 tttaatttca tatcatgaag ttataagtta tttattaatg cttagtcaat ttcgcacatt
       61 gaatttgatt ttttactaaa ttttgctaat ttttttatca attaactttt tacgtctata
      121 tttgataatt aaaatcaata ccatgtttca agaaatttat aatgatattc aagcaaaaat
      181 gcagcaacag atacaagaaa aatttggatt gagtgctgac caaacgacgc aaagtactaa
      241 tgtgttatta gataatttta aaaagttttt ttcagaagac ttgatggctg gtggcatggc
      301 aggtttgaaa gatataatga gcaatggcat ttctgatatt aaaaacaatc cgacaatgca
      361 gactttgcgc gagaatattg taaacgattt agtaaacaaa gtaggcttag cagaagaaac
      421 cgcacaaaag gtgagagact tttcgttgac tgaagtattt gataaattaa aaacagaatt
      481 tttagatgaa aatggcaaac cagatttttc taaaattatg agtaaaataa atatggcaga
      541 ctttcaaggc aaagctcaag agatgatggg caaaatggga atggacttcg gtaagttatt
      601 tggaaaataa gcgtgtattt atagcacttg ttccaatagt tttctgtaaa tttttaagcc
      661 atcttcaatt tcctgtaagt atataaattc atcggcagta tgggagcgct ctgattttcc
      721 aggacccatt tttatgcttg gtattttcaa taaagtttga tcagaaagtg ttgaagagcc
      781 aaatgtttca atgcctaatt tttctgctgc ttttacaatt aaatgttctt tatcaatttg
      841 cgaaggctgt aagcgagtag aacgcacttt aatatcagct tttacattgt cttttattat
      901 ttcaataatt tgctcgctcg tatattcggg aatagtacgc acatctactg tatatttgca
      961 tacatcaggt attacattgt gttgtgtacc tgcttgtatc atgcttaccg tcatttttac
     1021 tggacccaaa actggattaa tttttttgta ttgatagttt tgaaaccatt gcacatcagt
     1081 cattgccaaa tagatagcat taacaccttc attgcgtgcg gcatgaccag ctttgccttt
     1141 tacttcgccg tcaaatacca tgaggccttt ttcagcaatc gccattttca tttctgttgg
     1201 ctcgccaacg attgcaaact cacaagaaga ggtgatgtct tcaatttgtt cgattccatt
     1261 tttacctgaa atttcttctt cagaagtagc agcaaaaatg agattgtatt ttaaatcttc
     1321 tttatcgtaa aaatgtaaaa atgtagccaa taaggaaatg agtgcagcac cagcatcatt
     1381 gctacctaaa ccatataatt tatcatcttg cacctctgca ctaaatggat ctttcaccca
     1441 accattaact ggctttacag tgtcgatatg agagttgagt agtatggtag gttttaaaaa
     1501 attataatgt ttattttttg cccaagtgtt atgaagcttt gtatcgaaag gaattttttt
     1561 tgattttaaa aacttacgaa cgatatccgc tgctttatct tcttctttgc taaatgagga
     1621 aacaccaatg agtttttgta gcaactcaat aaactgtatc tgtgcatcgt cttgtatctt
     1681 cgtcatctta gtttttggta aatgttgttc caaatttagc attttcattt acatttgaaa
     1741 ggatattttg tgcatgacat aaaataactt tttccacacc gttttccaat gcatatttta
     1801 tattatcgat tttgggcaac atgccatcgg taatgatttg tttctctttt agttgttcaa
     1861 tttctgagct cttaacatgg ctaataatac tgtcatcatt gttaacattt tccagcaatc
     1921 catttttttc aaaacagtag taaaagctta cttttttatc tttagataat gcaatagcga
     1981 ttgccgcagc caatgaatcg gcatttgtgt ttaatagtaa gccatttttg tcgtgtgtaa
     2041 tgggagcaat aattggtgtt aattgctttt ctaataagga caaaaagaaa tccatgttta
     2101 cttcatcaat gtcgccaaca aaaccataat caatcaaatt atttacacgt ttgtgcgaaa
     2161 gaatgctatt tgcatcagca ccacaaatgc caatggcatt gcaattattt gcctgcaatt
     2221 gcgctactat ttttttatta atcaaaccag cataaaccat tgtaactacg tcgatggttt
     2281 tttcgtcagt tattcgtctg ccatctacta attttgattc gattcctaat tgtttactga
     2341 gttgcgtagc aatttttcca ccaccatgga tcaaaatttt tggagtattt atttttgata
     2401 aatcctgtaa gaattgatga agcgcttctt ccttatcaat aatatttccg cctactttta
     2461 taactagtat tctttccaat ttgtatattt atttttgtaa aaataaaact atgaataaaa
     2521 acttgcttat tgtttatgtt attctttttt caattttatc ttgtcaaaaa aatacaccac
     2581 aaaaactata ttcaaaaatt gtcacggcac ccggccctga agatatagta ttagataaag
     2641 cgaataacag aatattagtg tcctgtgatg aaagaagaac aggtatggct ttacaggctg
     2701 ccattatgca aatagatttg aataccgata tttgtacagc attaccgatt gtaaatatgc
     2761 catcaattcc atttcatcct catgggtttg atttgcaaac agtaaatggc atcgattatt
     2821 tatatataat caatcactat cgcgatagtg cctatacgaa tgctgttttg caatttgaaa
     2881 ttaaagcctc acaattggaa tttgtgcgtg agtataaaca tccattattg atttcaccca
     2941 atgatattac tgtattgcct aatggtgcat tttatttttc taatgataaa aattcttcca
     3001 atattttaga aatgctttat agtccttatg gtggctcggt ggtgtattgt aatggtgcaa
     3061 caacatggca aaaagtggat agtattatcg gttttccaaa tggtctttat gatgaaggag
     3121 atgctttgta tttagctact tcgcgtaatt atgcgatgta taaatatgaa atgcaatctg
     3181 atggaacgtt gaaaaataga aagcgaatta ctgcaattaa tggcatggat aatattatag
     3241 aaaatggcga cgatttaatc gtttcagtgc atcctgatga aataaagttt gctctgcttt
     3301 cctattttcc acagacattg tcaccttcaa aaacgtatgc tattaataag caaacaggac
     3361 aagcacgtct aatttttgat gatgacggaa aaaaaatttc tggttcatca acagctttgg
     3421 tgcacggtaa tgatttgtat ttagcacaag tttttgatgg ttttgtatta aaaattgcca
     3481 actataagta ttgaaatata atattgtagt agcatcttcg ttaataataa aaactagata
     3541 tgaaacacgg tattactatt ctgattgtat ttatgtatac actcggtttt tcaatagaaa
     3601 aaattcctga aacacctttt acagaatatc gttttaatgc tgctgagtgt aagctgatac
     3661 agcatcatca atctcgaatt tttattcctt catttgcttt ttatctagat ggaaaattat
     3721 atcatggcga tgtcgtgtta aaatacagag agtttgtcga tcagttagat ttaattctca
     3781 ataatattcc gatggcttat caggaaaacg gcaaacagca tgtgctggaa agtgctggta
     3841 tgtttgagtt cttagcgtat ggcaatggta aattattatc atttgctcct gctaaaaaag
     3901 cacaagtaca attggcaact gcatttaata tgacaggtgg tgaaacattt attttaaata
     3961 gacaaacgaa cacttggcga aaagacacac cgtttgcttc tttatcggaa tcgaatagag
     4021 agataggaga tgactctaac gcattatggc cagaaaatat atggaatgat gcagttaata
     4081 atgatgagat ttttgattcg tctggcaata caaatgtagc tatgtcatct tctaattcaa
     4141 atacactttc atttgaaaca ttaagtaatc aagcgttcaa gacattaaat gtagataaaa
     4201 tggaagtcta taattgtgat aaaattttga caggagaaac aattccaatt cttgctgatt
     4261 ttaatttgca aggttttaag caagctttac actcgcctgt atatgttgtt tataaaaata
     4321 gaaatgcggt tttgacttat tttccggaac agtttaaatc ggattttaaa ttgatagcaa
     4381 atgaagattt taccattttt acgttttcaa tggatggaaa aattgccgtg ttagacaatg
     4441 cgtttgctgc taattttgac gctaaaaatt atagaaataa aacagtctta tttccattaa
     4501 aagtgtttgc aaaggcgccg aatacaaaag aagaattagc aaaaattaca ggtttgtaag
     4561 cttaaagtgt attttaaaaa tgagaaaata ttttttgatt ttctttatgt tttcttctgt
     4621 aagttcagca tcggcacaac atttgtttgt tgctgacacg tttatttata ctattaactt
     4681 agatttgtct aaaaataatt atctaaaaac tgttccactt gatttgttga atggctattg
     4741 tcatggaaat tggaaggcat attatccaaa acgcacattg aatgaatgtt tattagatga
     4801 tttcttacaa cattttaatg tatttcaaat aaacactgca actttttcgt gtttagaaga
     4861 ctattgtgct aatccatatt ttaaagactt tttccaacag tttacacgta aacttaaata
     4921 cagcgaaata gttttttttg atcaacaaca ttcgttcgtc aaacgcgaga ttataagctt
     4981 gcaagttttt tattcacgta atgagtataa tggctggaag cattatgaag gtccgatatt
     5041 ttggatgcaa gaattgaaaa aatttcctga actaattaaa gtctataata aaaatgtacg
     5101 acctgatgct tggacattag aaaaagaatt taatcatgct ggatttatta ctaatgaaaa
     5161 taaacagaat gacgataaac agaaattaca gaaaatacta cagattgaag agtattaatc
     5221 ctcaatattt ttaagcttca aggaccaatc taaattcaat tcaacaatca atggaaaatc
     5281 agcatcatca acataagtga tttggtagtc gccgccatca ctaaccgttc tgcattgaaa
     5341 ttcgttagta taattatttt tatagccata gctttgtgta tgttggtatg gatttccgaa
     5401 aattgttttt acattttttt tcttgtctgt tacaatttcc attgaaccat catttttcaa
     5461 ttcttcgaac attttcttgc ttagaaaaat acaacattga tcgtccaaac ttatatcgcc
     5521 attggtaaaa tgactcaata tgccacttgc attttcgatg gctgctttgc taagctgtat
     5581 agtgcctttt ccatttgctg gcttagacat aaaccattca aaagtataat tttcattatt
     5641 attttttacg attacggtta cttcgtaaga ggcaccttcg aagttcacat cgtaaattaa
     5701 tttagaacct aaacgtatta catctttagg atttccatta gccaataaaa atgtaaaaat
     5761 aaatagaaat gctccagtga gcaaagcatg taaatttctt agcatattcg accattttga
     5821 aaaagtaatt taatacataa ttacttaatt gtcaataggt aatcacaaga aaaagcaatt
     5881 gattgccatt ataaattctc taatattttt tttaatactg cttgtgctgc gtattcgcgg
     5941 ttggcggctt gtttgatgac taaagaataa gcggaatcta acacatcatc ggcaatttct
     6001 aagtttctgc gtgttggtaa gcaatgcata aaattggcat gatttgtcaa ttgcattttc
     6061 ttatccgtaa tcatccaatc aggattatca cctaaaattt ttccataaga atcgtatgca
     6121 ctccaatttt tagcataaat aaaatctgca ttttcaaatg ccttatcttg catatattca
     6181 atgtgtgcat tgccactaaa tttttcattt aattcatatc ctttaggatg tgttattaca
     6241 aattctacat ctgcatgatt catccattca gcaaaagagt tggccacaca ctgcggtagt
     6301 ggtttgatat gtggtgccca gcttagcaca acctttggtt tctctttttt ggaatattca
     6361 cgaatggtca ttaaatctgt taaggattgc aatggatgaa ccgttgcaga ttccatatta
     6421 acaacaggaa catttacatt ctccaaaaat ttatgcaata cgtattctga ataatctttt
     6481 tctctatctg taagcgtagg aaatgcgcga ataccaataa tatcagcata ttcgctaatg
     6541 acagcagcac cttctttgat gtgttcagct gttgtaccat tcatcactgc gccatcttca
     6601 tattccattt gccaactatc ttgtccgaga tttaatacga tagtttgcat gcctaaattg
     6661 tgtgctgctt tttgtgtgga tatacgtgta cgcaaagaag aatttaaaaa caataaaacc
     6721 atcgttttgt ctttgccaat agatttattt gcaaattttt gttgcttaat cgcaatggct
     6781 tcttgtgcta attgttcggc atttacaaca tcatgtatgt ttaagaaatt tttcatttaa
     6841 gcaaactatt taatgcattt attaattggt caatttcttt tttagtaaca gttagactcg
     6901 gtaaaaaccg catggtgttt ggctggtttg agttgcctac aaatatgtga tgtttgtaca
     6961 ctaatttatc tcttatttct ttaattggaa aatcaaattc tacaccaatc attaagcctt
     7021 ttccgcgaat ttctttgacg tgtggcaatt gctcacattt ttcttttaaa taatttccca
     7081 tgttgatggc attatcaatt aaatgttcat tttctatgac atctagtaca gccattactg
     7141 ctgcacatgc tagtggtgcg ccgccaaaag tagtgcctaa catactgtat tgtgcaataa
     7201 tttttggatg gataagaatg cctgcaattg gaaaaccatt gcccatacct tttgccatgg
     7261 tgatgatgtc tggttgcaca ttcgcatatt gatgtgcaaa aaattgacca cttctaccaa
     7321 agccacattg tatttcatct gcaataaata aggcgccatg ttctaaacat aatttttgta
     7381 tcaattgtag aaaggcagca ttcggctcat aaatgccatt tacaccttgt attggctcaa
     7441 tgattacggc acaaatttca ttggatttaa aagcattttc taagcttgct tcatcattaa
     7501 aatctaagaa aataacatgt gcgtcgtcat tgattggtgg tttaattttt ggattatctg
     7561 taaccgcaac ggcacctgat gtcctaccat gaaatgcgcc tctaaacgcg attacttttt
     7621 tacgattatt atagaaagaa gctactttta aagcattctc tacagcttct gctccgctat
     7681 ttatcaaaaa tagatgataa tcctcgtagc ccgaaagctt acctaattta gtggcaactt
     7741 gttgttgtat ttctaattga acagaattgg aatagaaagt taatcggtgt aattgttcgt
     7801 ttagtttttg aatataatgt tcatgtccat gaccgataga aattacagcg tggccaccat
     7861 aaaaatcaag atattctgtg ttgtttttat catacacata acaacctttt ccacgctcta
     7921 gttgtatatt aaataaagga taaacgtcaa atagtttcat tttttttaga attgtttttt
     7981 attaatacgc aacagatttt agttttaatc ctgtagtttc atcaagtgca aacatcaagt
     8041 tcatattttg aacggcttgc cctgatgctc ctttaactag attatcaatc atagaaatta
     8101 tcattaattg attttcatgt ttctctaaat aaagcaagca tttattggtg tttacgactt
     8161 gttttaagtc tatattttta ttagaaatta ctacaaaagg ctcatcgcgg taatagttta
     8221 taaaaagctg ttttgcggca tctaatgaat ctttaaattc aacatagata gttgctagaa
     8281 ttccgcgtgt aaaatcacca cgataaggaa tgaattttat ggaagcatca aatgattctt
     8341 gaagctgatg caaactttct tttatttctc ctaaatgctg atgtgtaaaa ggtttataga
     8401 ctgaaaaatt attatttcgc catgtaaaat gcgatgtttc gctcagcgaa acaccagcgc
     8461 ctgtgctgcc tgtaacagca gaaatatgaa tgtcgttttg caataaattt tcttttgcca
     8521 atggtaataa tgccaattga atagctgtcg caaaacaacc aggattggca aggtgctttg
     8581 cgttttttat ctcatttttt ttcaattctg gtaagccata aacaaaatta ttcgcatttt
     8641 ttttcaatcg aaaatcattg cttaaatcaa tgattttggt agtaggtgag aagctattat
     8701 tttcaataaa atgttttgat tcgccatgtg gtaaacataa aaaagcgaca tcaatttcct
     8761 tgctcaattc atttgtaaaa tgtaaatcaa tatcgccaat taaatcagta tgtacatcat
     8821 atattttttt gccgtgctga cttctgctat aacagtactc gatgttgaca tttggatggt
     8881 gtaataaaat tcgtattagt tcgccaccag tatagccagc agcaccaata atgcctactt
     8941 ttattttgtt atttatttcc atttttcacc taatttattt ccaacaaaaa tccataaata
     9001 gccacgaatt ctgttcgtgt aatatttaat tgctcgaacg tggttctaaa ttaattgaaa
     9061 tatcatagta aaatggaaat gacaagtaat atttgtgtta aacgttattt gttggtcgca
     9121 aaaatataat cattaaaaat ttttatacta gacaaaaatg attgatttgt tactttatca
     9181 ttcgttgctg taaaacgtat tttaatggtg tctttgttga aatctggagt aatgaaatca
     9241 tcttcaatac tgttgatatc attgattgct aagtagtgta ttaaatgatt gattatttgc
     9301 ttggcttcta aaatggtatc aatttttact gaatttgtaa ttgatgttgc tttctcaaaa
     9361 gatgttatta atgcttttct gttgtaggtc aatgtttgtt ccatagttat tatttttatg
     9421 tataacaacg aaagctggaa aaaaattcca ttttatagca aatagaatgc taaaaataaa
     9481 ttagaatgaa aattgactta tacgtggaaa atagttgact aacgtttgcc catgcccatg
     9541 ccttgcatac gtttcatcat cactttcatt tggtcaaatt gttttacaaa tgcattgacc
     9601 tcttgaatag tacttccaga gcctttcgcg attcgttctc tacgttttcc gtttaacaaa
     9661 tcaggatttc tgcgttcttc aagagtcatg gagaaaatca tagcttcaat ttttttgaag
     9721 gcattgtcgt cgatatcgat gtctttaact gctttaccaa cacctggaat catacctaat
     9781 aaatctttga tatttcccat tttgcgaatt tgattgattt gctgtaaaaa atcttcaaag
     9841 tcgaattggt ttttagcgat ttttttctgt aaacgttgtg cttccttttc gtcaaattgc
     9901 tcttgtgctt tctccacaaa agacacgatg tcgcccatac caaggattcg ttgcgacata
     9961 cgttcaggat agaaaatatc taaggtgtcg attaatttct cgccacgaga aacaaattta
    10021 ataggcttat ttacggtgta tttaatagac aaagctgcac ctccacgcgt gtcaccatct
    10081 aatttagtta agacaacgcc atcaaaatta attctatcgt taaatgcttt tgctgtgttt
    10141 acagcatctt gaccagtcat ggaatcaaca acaaatagtg tttcttgtgg ttgtatagct
    10201 tgtttaactt gatagatttc attcatcatt tcttcatcaa cggctaatcg accagcggta
    10261 tcgacgatga caaagttttg tcctttttgt tttgcatacg caatggcatt ttgtgctatg
    10321 gcaacaggat ttttattttc aatttccgaa tatacttcca cgccaatttg ctcacctaat
    10381 acttttaatt gatcaattgc ggcaggacga tatacgtcac atgctaccaa taatggtgtt
    10441 ttgttgagtt ttgtttttgc gtagtgtgct aattttccgg agaatgtggt ttttccagaa
    10501 ccttgcaaac cagaaattaa taaaacagca ggagagcctt ttatattgat ttcactggca
    10561 gttccgccca ttaattgcgt caattcatca ctcacaattt tcaccattaa ttgtcctggc
    10621 gatactgctg ttaaaacgtt ttgtcctaaa gctttatcct ttatggtgtc tgtgaattcc
    10681 tttgcaattt tatagtttac gtctgcatca accaatgcac gacgaatgtc ttttactgtt
    10741 gcagcaatat tgagttctgt aagctttcct tgacctttta ggtttttaaa tgcaccttcg
    10801 agcttatcct gtaaattttc aaacatgatt acatattgta aattagctaa ttagctaatt
    10861 agtgaaataa ttttcttaat aatacgtcaa acgctgtacc ttacctaagt attttgccaa
    10921 tctaattacc tgacgtgtat aaccatattc attatcatac caaacatata acacgattga
    10981 ttttttattt ggcgaaatta atgttgcatg tgaatctaag atagaagcat gtgaattgcc
    11041 tacacagtca gtagatacta attcttgaga agttgaatag tcgatttgct ctactaaatt
    11101 tccaaatgta gccgcttcac gaataattgc atttactgca tctattgatt caacagcagt
    11161 agataagttt aggtttaaaa tagctaatga aacatctggt gttggcacgc gtactgcatt
    11221 tccagtaaat ttgccagcca tacttggcat tactttcgtg attgcactgg ctgcgcctgt
    11281 ttcagtgatt accatattta atggagcaga acgaccacgt ctgtagcttt tgtggtaatt
    11341 gtctaataaa ttttgatcgt tagtatatga gtgtactgtt tctacgtgtc cactttcgat
    11401 gcctaattta tcagcgatta cttttataat tggagaaata gcgttagtgg tacaagatgc
    11461 agcagtaaaa atatttgtag catctggatt gaaatctaaa tggttgacgc catatacgat
    11521 attgggtaag cttcctttgc caggtgctgt caacaatact ttagcaattc cttttgcttt
    11581 taagtgcgtg cttaattttt cttcatctct gaaaatacca gagttgtcta ttaacaatgc
    11641 atctttgatg ccatattgag tatagtcaat ttccgcagga ttatctgcaa caatcatgtg
    11701 tattttgttg ccatttgcta ctaatgcttt attgtcaaaa tcttctgtaa taacaccacg
    11761 gaattttcca tgcacagagt ctttacgcaa taatgaagaa cgttttttga tttcttcatc
    11821 tgtatttttg ccacgtgtta ctattgcttt taataccaat tgctcgccat tgccatattg
    11881 ctgaattaat tcgcgagcag ctaaacgacc aatacgacca aaaccaaata atactacatc
    11941 tatcggttta ataacgcgtt tatcttttcc aatgtagttg gctaattttt cgagaacaaa
    12001 agcatcaaag tccgttacat ttgcttctaa tgcttctttt gctaaagtag ctaagtcgat
    12061 gcgtgtaggt gctaaatcta attttgcgat ggcacttgca atgttgttta gaagctcaat
    12121 agttaaaggt aaacctaaat attttttagc atattcttta tggtttaaca cttcgctata
    12181 acgtgcatct acaatgtttc ttctgataac gactaattca atatttcttt ctaaccaaag
    12241 taagcctgct gtatttataa gttctaatgc agttttttct ttagaattcc aattgcccat
    12301 tttttttaat ttttatgtat gtatgtttaa agaatgaaag atatcaaagt aagtgaaaag
    12361 cagattataa aaaaaagatt aattgcctaa ataatttttt aataatttac ttcttgatgt
    12421 agcacgtaat ttattaatgg ctttgtcttt aatttgacga acacgctcac gcgtaagtcc
    12481 aaatttttcg ccaatatctt ctaatgacat tggatgttca acgccaatgc cgaagtaata
    12541 tttaattaca tctttttgtc tgtctgttaa tgtagataaa gaacgttcaa tttctttgcg
    12601 taatgattcc aaatattcta attgtaaatc tgttcgaggt gcagatgtgt tttctaatac
    12661 atctaataat gaattttctt caccttcttg gaaaggagca tctacagaaa cgtgacgagc
    12721 tgctacgcct aatgttgttt cgacttcttc acgtgtgatt tctaacattt ctgctaattc
    12781 atcagcagac ggctcgcgct caaattcttg ttctaagttc gaaaatgctt tattgatttt
    12841 atttaatgaa cctacttt
//
LOCUS       DAOQQV010000073        12613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1724_length_12613_cov_6.550088, whole genome shotgun sequence.
ACCESSION   DAOQQV010000073 DAOQQV010000000
VERSION     DAOQQV010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1724_length_12613_cov_6.550088"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLL59_08330"
     CDS             <1..913
                     /locus_tag="PLL59_08330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY23854.1"
                     /translation="KEKIKIRKMINTIHPKNFKTYELIDSGNFEKLERFGEYITIRPE
                     PQAVWSKRLSEEEWKKQAHVKFVGLSSSTGKWEFFKKMPENWPLSYKHGQLSISFNLA
                     LTKFKHLGIFPEQSVNWDFIFEKINNIKNNVPQPKVLNLFAYTGGASLAACAGGAETY
                     HVDSIKQVVSWANRNMESSQLKDIRWVVEDAVKFVKRERKRGKIYQGIILDPPAYGHG
                     TDGEKWKLEDQINEMLEDVLHLLDKENHFFILNTYSLGFSALIIENLIHDNLKNAGFS
                     NVNPEFGELFLPSTSGQKLPLGVYCRF"
     gene            complement(1048..1815)
                     /locus_tag="PLL59_08335"
     CDS             complement(1048..1815)
                     /locus_tag="PLL59_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMY23855.1"
                     /translation="MITTLIVEDDPIFRDMLTDMLPKSSVKTNVIESCATVRQAKVAI
                     DHHQPQLILLDVELPDGKGMDLLQHYEEFGTFETIFVTSHDKYAIDAIKKNAADYIVK
                     PVQLEELNHALNKVKKRLDTYSVLIKVDELTSYVEKLKHQNLQEKKIMINTNEGAIFV
                     KVSDIIKLESESNYTILYLDNNKKIIASKTLSVFEAMLEELNFMRIHRSFVINLTKIK
                     NIENEAGCYHVNMSDASRVEVSRRKKKDFFEKIAYHI"
     gene            complement(1964..2236)
                     /locus_tag="PLL59_08340"
     CDS             complement(1964..2236)
                     /locus_tag="PLL59_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23856.1"
                     /translation="MKKITTTANATINTIVACVAKAKTFQTSKVFLFVCIATAMLFFS
                     SCARNSTGCGNGTWACKNSNKYKVDKKGAKYMANIYKCPSNQQYSR"
     gene            complement(2338..3354)
                     /gene="hemE"
                     /locus_tag="PLL59_08345"
     CDS             complement(2338..3354)
                     /gene="hemE"
                     /locus_tag="PLL59_08345"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HMY23857.1"
                     /translation="MLKNDLLLKAARGEQTERIPIWLMRQAGRILPEYRAVRSSVHGF
                     KELVKNPELICEVTLQPVDILGVDAAIIFSDILVIPEAMGLNYEMIESKGPFFPKTIQ
                     NAQDISVLTTDVVPNLQYVFESIRLTKAALNNRVPLIGFAGAPWTIFSYMIEGKGSKT
                     FSEAKKMLYVNPVLSHQLLDKITSATIAYLKEQIRNGADIVQVFDSWAGVLSKAQYIE
                     FALPYMQKITEAITEVPVILFAKDAHYIVKEFSQTNTSVIGLDWTIDPKQARMEAPNK
                     TLQGNLDPCVLYADKETIKAETHKMLQAFGHQNYICNLGHGVYPDVDFEKAKYFIEVV
                     QSYR"
     gene            3436..4167
                     /locus_tag="PLL59_08350"
     CDS             3436..4167
                     /locus_tag="PLL59_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMY23858.1"
                     /translation="MAEVIEINNPTVSKIMPRVVDIKNAKIMHHQHLVLDKVNFEVRK
                     GDFVYLIGRTGSGKSSLLKTLYGDLEFEHGEADVCGYNLKTLKKSDIPYLRRRLGIVF
                     QDFQLLSDRNVFDNLDFVLRAIGWKDANKIKTTIEESLEMVKLTDKLKKLPNQLSGGE
                     QQRVVIARALLNHPELILADEPTGNLDPETTDEIMSLFYSIFKATQTPMVFATHNYQL
                     IEQYPGIIYMCAYNSISRDDSHFTR"
     gene            complement(4170..5225)
                     /locus_tag="PLL59_08355"
     CDS             complement(4170..5225)
                     /locus_tag="PLL59_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMY23859.1"
                     /translation="MNKELATIRIILIFFAAVAVLFLIHLLQELLVPLMLAMFIALLM
                     QPSLVWFDKKKFPLWLSVSMIWIFLFVVCFTIGAVIYKTGSAIFAEKDFIVSQVQIKT
                     QGLLQFYNKFTGKELDLSVVMDKAMSSISSEFIMNNSSSIFGKLGTLFEEFLLTAIYL
                     VLILSGIMRYENYLDYLGGEGKSTKYIIAFEEIKSSIVSYMKVKFIISVLYGLGVTII
                     CWMFGLQFAFFWGFIGFILNFLPVFGAIIGLVPVFIMALIQFDTVFTAIMLNVVVYAY
                     HFILASVIEPIFMGNRTSLNTIVIIMGLLFWGYLWGIYGMFLSVPMMVLTKVILSQIE
                     GTEVIVRLLGTQSEQKQ"
     gene            complement(5226..6176)
                     /locus_tag="PLL59_08360"
     CDS             complement(5226..6176)
                     /locus_tag="PLL59_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HMY23860.1"
                     /translation="MIKIYKNIGDELQLIEQPEQDCWINIAPPFNMEQLERLANQLEV
                     PYSYIVDCIDQYERSRYEKQGTTKLIVINTPILNEGFDMEDEATYITIPIGLIILPTM
                     LLTVCSVNNPMIEWFEKNVLRNVQLSNRNLFVIRIFERNIYYFLHYLREINKRISQIE
                     KELNYSSRNQELNKLLHLQKALIYFVNDLRANELVLVKIQRNDFLDLKEDNFSKELVQ
                     DIIIDNSQALEMANVYSNILGNMMGAFASIISNNLGHVVNRLTAVTIALMLPTLVAGV
                     YGMNIDLPFQHHPHAFTIVIILSVLVSISFVVFFRKKRWL"
     gene            complement(6192..6974)
                     /gene="dapF"
                     /locus_tag="PLL59_08365"
     CDS             complement(6192..6974)
                     /gene="dapF"
                     /locus_tag="PLL59_08365"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMY23861.1"
                     /translation="MQIDFLKYQGTGNDFILIDNRLLFFPKENTQLVHQFCDRKFGIG
                     ADGLMLLENASGYDFKMVYFNADGNESTMCGNGGRCIAAFATHLGITQKLGKFIAIDG
                     EHDFIIENNAVRLKMIDVPAIYKDDITNDFVLFTGSPHYVRFESDIDNIDLVSEAKKI
                     RYNEVYAKEGINVNFVEANDSGIKMRTYERGVEDETLSCGTGTVAVALSYAVRQQINK
                     GKIDISTPGGHLAVSFELKDNIFTDIWLEGPASYVFKGNFSI"
     gene            7108..8598
                     /locus_tag="PLL59_08370"
     CDS             7108..8598
                     /locus_tag="PLL59_08370"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HMY23862.1"
                     /translation="MKRISSYFIIGIISAVTAIAISHFLYKKNATIISEEKSGPFKFA
                     SYNSALPADAFVEAAKTSTPTVVHINTIIKTKKSDRNSKYVNPFYQFFGDPFGNSPYD
                     MPPQQDQEASGSGVIISNDGYIVTNNHVVENADEIKVSLYDNREFSAKVIGTDPNTDL
                     AVIKIDGKDLQFLTFANSDNVQVGQWVLAVGNPFNLSSTVTAGIVSAKTRNINILREK
                     AGNLAIESFIQTDAAVNPGNSGGALVDLSGNLIGINSAIATPTGSYAGYSFAIPANLV
                     NKVVKDMIDFGVVQRGFLGVSIREIDDELAKDLKLNKIQGAYIAEVNKGSAAEDGGVK
                     RGDVIMKVGDAEIKNSADLQEHVARYRPGDKVKLSIFRDGNMIAKDVVLKSKDNKTAL
                     LSKEDAPKTGNVLDNLGIAVDELSSLEAKKLGVSGGLKVTKINDGIIKQNTSMQEGFI
                     IIGINNRSVTKKSDLIDLINESKGNGILLQGKYPDQNGLKYYAFGY"
     gene            8681..9391
                     /locus_tag="PLL59_08375"
     CDS             8681..9391
                     /locus_tag="PLL59_08375"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMY23863.1"
                     /translation="MANTYFQFKQFTIHQDKCAMKVCTDSCVFGAWIPIPKNTQHILD
                     IGSGTGLLSLMMAQRTNAIIEGIEIENNAYLQSVENFNNSKWSDRLHMHHGNIKDFAF
                     NKKFDFIICNPPFYENEKSSENTEENIAKHSMHLTLEELIFAIKNIVSEHGKFAILLP
                     YFRKQELHSICHQNNFFLENEIDMRQTPKHDFFRYAAVFTTQKNPKVSQEEITIKDAH
                     NHYTADFVKLMQTFYLKL"
     gene            9477..11327
                     /locus_tag="PLL59_08380"
     CDS             9477..11327
                     /locus_tag="PLL59_08380"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMY23864.1"
                     /translation="MKKYLLLICALYFTASFAFAIEPISSTERSMVDTSLVPFYHGVA
                     SGDPTANAVVIWTRVTPETAGIVNGTWRMALDTNFTSIVQSGTFSADSTTDYCVKIDV
                     TGLQANTWYFYEFTALGRNSLTGRTKTAPISGVNQLRFAFVSCSNYPTGYFNVYNRIK
                     DRNDIDAVLHLGDYIYEYGTNPLSTRTDQMPNHEIIQLADYRQRYSTHRLDPALRKLH
                     QQYPFITVWDDHETANNSYTNGADNHTPGSEGPWSVRKAAGQQANDEWLPKRLQDAHN
                     TNKIWRKISYGNLADIFMLDTRLYDRSIMGGNTNDTSRHIIGNEQMEWLKNELIHSTA
                     KWKILGQQVMMGNLTPFGIMLNNDQWDGYNADRKKLYDIILNNNIKNVITLTGDIHTA
                     WAMDLPYNTATYNPATGAGSVAVEFVCTSVTSSSSPIPLDPIYGLVTSLLPHIKYVDL
                     FKKGYGILDLKDTIAQGNFYSVPTVSTLNTGQAFETAYYTLENTRWLKKTTTASVMLA
                     PPFYQAPETPRQTVVSGIKNRSNDLIVLSAYPNPFIDQITIQYNTASNTALSLNIYDF
                     SGRLVKTQDLGFHSKGLYNKTISLSDLASGAYKIVLSNNNEIIERTIEKY"
     gene            11330..12106
                     /locus_tag="PLL59_08385"
     CDS             11330..12106
                     /locus_tag="PLL59_08385"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HMY23865.1"
                     /translation="MAIIKKTTTKPVKKNLATKKSVTKKVTPKKATSLKSVTKKPQAQ
                     KPKPIVKSVIQAEKTDPKIFPIGKFSVPHEYTDVVINNLTQELKSLPKDLKKVAKKIK
                     SDAQLQASYRDGGWSIEQIIHHLADSHMNAFIRFKLSLTENNPVIKPYNQDLWAETAD
                     IQLPIKISIKLLKALHTKWVCLLENMDKNDFKRTYTHPEYKTTPALQEILAQYAWHGK
                     HHLAQIELALNSIEIEKVDEEKKSKESKSIKPKSKPARKH"
     gene            complement(12132..>12613)
                     /locus_tag="PLL59_08390"
     CDS             complement(12132..>12613)
                     /locus_tag="PLL59_08390"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HMY23866.1"
                     /translation="RVFFIEVMGRDVGSIALAAGLAAGAEDILIPELKTDIHELSLKL
                     QNSRQKESYIVVVAEGDDAGGVLEIAAKFKQLHPDYDVKTLVLGHLLRGGSPSANDRI
                     LASRLGTAAVDALLNKNFNVMVGVEKNSICYTPLSQIRKHFVSINEEWKHLLDVLSY"
BASE COUNT         4459 a         2128 c         2078 g         3948 t
ORIGIN      
        1 aaaagagaaa attaagatta ggaaaatgat taatacgatt catcccaaaa attttaaaac
       61 ttacgaactc atcgattccg gtaattttga aaagctggaa cgttttggag aatatattac
      121 catacggcca gaaccacaag cagtttggag caaaagattg agcgaagaag aatggaaaaa
      181 acaagcacat gtaaaatttg ttgggctttc ttcatcgaca gggaaatggg aatttttcaa
      241 aaaaatgccc gaaaattggc ctttatctta taaacacggt cagctttcta ttagttttaa
      301 tttagcgctt actaaattta agcatctagg tatttttcct gagcaaagcg ttaattggga
      361 ttttattttc gaaaaaataa acaacattaa aaacaatgtg ccgcagccaa aagtattgaa
      421 tttatttgct tatacaggag gtgcaagcct tgctgcgtgt gctggcggtg cagaaactta
      481 ccatgttgat tctataaaac aagtggtaag ttgggcaaac agaaatatgg aatcatcgca
      541 attgaaagat attcgttggg tggttgaaga tgctgtgaaa tttgtaaaac gcgagcgtaa
      601 acgtggaaaa atttatcaag gtataattct tgacccgcca gcttatggtc atggtactga
      661 cggtgaaaaa tggaaactag aagatcagat taacgaaatg cttgaagacg tattgcactt
      721 actggataaa gaaaatcatt tttttatcct caatacttat tcactcggat tttccgcatt
      781 aattattgaa aatctgatac acgataattt aaaaaatgct ggtttttcta atgtaaatcc
      841 tgagtttggc gaattattcc ttccttcaac atctggacaa aagttgcctt taggtgtgta
      901 ttgcaggttt taaaaaaaat taaaaaaaag aaggtgcttg tagaaaacaa gcacctctta
      961 gaaacataaa acccgttgag agatatacta tatataacga gatttattgc tgattaattt
     1021 cctgacttcc attaaaaaat gttaaaatca aatatgataa gcaattttct cgaaaaaatc
     1081 tttctttttc cttcttgaca cttccactct tgatgcatct gacatgttta catgatagca
     1141 gcctgcttca ttttcaatgt tttttatctt agttaagtta attacaaatg aacgatgaat
     1201 acgcataaaa ttcagttctt ccaacatagc ttcaaataca gaaagtgttt tagatgctat
     1261 aatttttttg ttattatcta aatacaaaat tgtataatta ctttctgatt ccagcttaat
     1321 aatatctgat acttttacaa atatggcacc ttcattggtg ttaatcataa tcttcttttc
     1381 ttgcaaattc tgatgtttca gtttttcgac ataagaagta agctcatcta cttttatcaa
     1441 aactgaatat gtatccaatc gtttttttac cttatttaat gcatggttta gctcttcaag
     1501 ttgaacaggc tttacaatgt aatctgctgc attctttttt atagcatcta ttgcatattt
     1561 gtcgtgtgac gtaacaaata tagtttcaaa cgtaccaaac tcttcataat gttgtagcaa
     1621 atccattcct ttgccatctg gcaattccac gtctaataaa attaattgtg gctgatgatg
     1681 gtcaatagct actttggctt gacgcacagt agcacacgac tcaataacat tggtctttac
     1741 tgaagatttg ggtaacatat ctgttagcat gtccctaaag atgggatcat cttctactat
     1801 taacgttgta atcatagtct tgttggttag tttaaagtta taaatatttt ctaaaaaatg
     1861 caattaactt atgattaata ttttgtgatt tgtatagata aaaaccgaaa gaaagcaata
     1921 gaatactttc tttcggcatt aactaacaaa aaaccgatgt accctagcgg gaatattgtt
     1981 gattagaagg gcatttgtat atgttggcca tatacttagc tcctttttta tccactttat
     2041 acttgtttga atttttgcaa gcccaagttc cattgccaca acctgtactg tttcgtgcgc
     2101 atgaactaaa aaatagcatg gctgttgcta tacagacgaa taaaaaaact tttgaagttt
     2161 ggaaagtttt tgcttttgct acgcaagcaa cgatagtgtt tatggttgca tttgctgttg
     2221 ttgtaatttt tttcatgttt ttgatttttg tactgtaaac ttacagccaa gcacaaaaac
     2281 acaacaattt gcatgagtaa acggcagatt tgatttgtaa atggtaaaat agctgattta
     2341 ccgataggac tgtacaactt ctatgaagta tttcgctttt tcgaagtcaa catctggata
     2401 aacgccatgt cctaaattgc aaatataatt ctgatgacca aaagcttgta gcattttatg
     2461 cgtttctgcc ttaattgttt ctttatctgc atataaaaca cacggatcaa gattgccttg
     2521 caaggttttg ttaggcgctt ccatacgcgc ttgttttgga tctatagtcc aatctaagcc
     2581 gataacggaa gtatttgttt gtgaaaattc ttttacaata taatgtgcat cttttgcaaa
     2641 taagataaca ggcacttctg taatagcttc cgtaattttt tgcatatacg gcaatgcaaa
     2701 ttctatatat tgtgctttac tcaacacgcc tgcccaacta tcaaaaactt gtacaatatc
     2761 agctccgttt ctaatttgtt ctttcaaata agcaatggta gcacttgtaa ttttatctaa
     2821 taactgatgc gacaaaacag gatttacata cagcattttt tttgcttctg aaaaagtttt
     2881 actgcctttt ccttctatca tataggaaaa aatcgtccac ggtgcgcctg caaatccaat
     2941 taatggaaca cgattattta atgctgcttt tgtcagccga atagattcaa aaacatattg
     3001 taaattagga acaacgtctg ttgttaatac tgaaatatct tgcgcatttt gaatagtttt
     3061 tggaaaaaat ggacctttgc tttcaatcat ttcatagttc aagcccatgg cttctggtat
     3121 tactaaaata tcagaaaaaa taattgcagc atcaacacct aaaatatcta caggttgtaa
     3181 agttacttca caaataagtt ctggattttt tactaattct ttaaaaccat gtacactgct
     3241 gcgtactgct ctgtattctg gtaatattct tcctgcttga cgcattaacc aaatgggaat
     3301 tcgctctgtt tgttcgccac gtgctgcctt tagcaataaa tcatttttca acataatggg
     3361 caaaaataca aattcttaca cttttatttt tcttatttaa tgtcgtactt tgtaaattga
     3421 cgaaaataaa atataatggc tgaagtaatt gagattaaca atccgactgt atctaagata
     3481 atgcctcgtg tggtggacat taaaaatgca aaaattatgc atcaccaaca tttagtatta
     3541 gacaaagtaa attttgaagt aagaaaaggt gattttgttt atttaattgg aagaacaggc
     3601 agcggaaaaa gtagtttgct aaaaacatta tatggcgatt tggaattcga gcacggtgaa
     3661 gcagatgtat gtggttataa tttaaaaact ctaaaaaaga gtgacattcc atatttgcgc
     3721 agacgattag gtattgtttt ccaagatttt cagttattgt ctgatagaaa tgtatttgac
     3781 aatttagact ttgtattgcg tgcaataggc tggaaagatg ctaataaaat taaaacaaca
     3841 atagaagaat cgctggaaat ggtgaagctg actgataagt taaaaaaact accgaaccag
     3901 ctttcaggtg gcgaacagca acgtgtggta attgctcgtg cattattaaa tcatccggaa
     3961 ttaatattgg cagatgaacc aacaggaaac ttagatcctg aaacaactga tgaaattatg
     4021 agtttgtttt attccatatt taaggcaaca caaacaccaa tggtttttgc tacgcataat
     4081 tatcaattga tagaacaata tcctggaatt atttacatgt gtgcttataa ttctatcagc
     4141 cgcgacgatt cgcattttac acgataatat tattgctttt gttcgctctg cgtacctaaa
     4201 agtcgtacta taacttcggt gccttctatt tggctaagaa taactttggt taacaccatc
     4261 attggcacag ataagaacat accataaatt ccccaaaggt agccccaaaa tagtaaaccc
     4321 atgatgatga caattgtatt taatgaggtt ctattgccca tgaatattgg ttcaatcaca
     4381 gaagctaaaa taaagtggta agcatatact actacgttta gcataatggc tgtaaacaca
     4441 gtatcgaact gaattaatgc cataataaat acaggaacta agccgattat agcaccaaaa
     4501 actggtaaaa aattaagaat aaaaccaata aatccccaaa aaaatgcgaa ttgtaatcca
     4561 aacatccaac aaataatggt aacgccaagt ccatataata cgctgataat aaatttcact
     4621 ttcatatacg acacaattga cgattttatc tcttcaaaag ctatgatgta tttagtgctt
     4681 ttgccttcgc cacctaagta atctaagtaa ttttcgtagc gcataatacc tgaaagtatt
     4741 agcactaaat agattgctgt caataaaaac tcttcaaata aggtgcctaa ttttccaaaa
     4801 atagaagaag aattattcat aataaattcg gaagaaatac ttgacatagc cttgtccata
     4861 acaacagata aatcgagttc tttgcctgtg aatttgttgt aaaattgtag taaaccttgc
     4921 gttttaattt gtacttgtga aacgataaaa tctttctctg caaaaatagc tgagcctgtt
     4981 ttataaataa cagcgccaat agtaaaacaa acgacaaata gaaaaatcca aatcatgcta
     5041 acacttagcc aaagtggaaa tttcttttta tcaaaccaca ctaatgatgg ctgcatcagc
     5101 agtgcaataa acatggccaa catcagtggc accaataatt cttgcaatag atgaattaga
     5161 aaaaggactg caacagcagc gaaaaaaata agaatgatgc gtattgtggc gagttctttg
     5221 ttcatttaca accatctttt ttttctaaaa aacacaacaa aagaaataga aacaagaaca
     5281 cttaatataa ttacaatcgt aaaagcatgc ggatgatgtt gaaatggcaa atcgatattc
     5341 atgccataca cacctgcaac aagcgtaggc aacatgagtg cgatagtaac tgcggtgagc
     5401 ctattaacga catgtcctaa attgtttgaa ataattgacg caaatgcacc catcatattg
     5461 cctaagatat tagaatacac atttgccatt tctaaagctt gcgagttatc gatgattata
     5521 tcttgcacta attctttgga aaaattatcc tcttttaaat ctaaaaaatc atttcgttga
     5581 attttaacta gtaccaattc atttgcccga aggtcattaa caaagtaaat cagtgctttt
     5641 tgcaaatgca ataatttatt gagttcttga ttacgactcg aataattaag ttctttttcg
     5701 atttgcgaaa tgcgtttatt tatttctctt aagtaatgta aaaaatagta gatattgcgt
     5761 tcaaatattc gaatgacgaa taaatttcta ttactcaatt gtacattgcg aagtacattt
     5821 ttttcaaacc attctatcat tggattatta acagaacaaa ccgttagcaa cattgtcgga
     5881 agtataatca aacctatcgg aatagtaatg tatgttgctt catcttccat atcaaagcct
     5941 tcatttaaga taggcgtatt gattacaata agttttgtag tgccttgttt ttcatacctt
     6001 gagcgttcat attggtcaat acagtcaaca atgtacgaat atggaacctc tagttgattg
     6061 gctaaacgct cgagctgttc catattaaac ggaggagcaa tattaatcca gcaatcttgt
     6121 tcaggttgtt caatcaattg caattcatcg cctatatttt tgtatatttt tatcataaca
     6181 caaatgcaaa cttatatact gaaattgcct ttaaacacat aagaagcagg accttccagc
     6241 caaatatctg taaatatatt atcttttaac tcaaaactaa ctgctaagtg tccaccaggt
     6301 gtagatatgt ctattttgcc tttatttatt tgttggcgca cagcataact gagtgcaacg
     6361 gcaacagtac ctgtgccgca agatagtgtt tcatcttcaa cgccacgctc atacgttctc
     6421 attttgatgc cactgtcatt cgcttccaca aaattcacat tgattccttc ttttgcatac
     6481 acctcgttat agcgaatctt ttttgcttcg cttactaaat cgatgttgtc gatgtcagac
     6541 tcaaatcgta cataatgtgg cgaacctgtg aaaagcacaa aatcattggt gatatcatct
     6601 ttataaatag caggaacatc aatcattttt aaacgaacag cattgttttc aataataaaa
     6661 tcgtgttcac catcaatggc aataaatttt ccgagctttt gagtaatacc taaatgtgta
     6721 gcaaatgctg caatacatcg gccaccgttt ccgcacatcg tactttcatt tccatctgca
     6781 ttgaaataca ccatcttaaa atcataacct gatgcatttt ctaacaacat taatccatcg
     6841 gcgccgatgc caaattttct atcacaaaat tgatgaacaa gctgagtgtt ttcttttgga
     6901 aaaaataata gtctattgtc aatcagaata aaatcattgc cagtgccttg gtattttaaa
     6961 aaatcaattt gcatacttca aaaatacatt aatcatagaa aaaagtagaa aaatatatga
     7021 ttaacttttt ttaacccaag tttaagatac tttcacgaaa aattttgttg taatgtatga
     7081 actttaatat tcacgaaaaa aagagatatg aaaagaataa gtagttattt tattattgga
     7141 attattagtg cagttacagc cattgctatt agtcattttt tgtataaaaa aaatgcaact
     7201 attattagtg aagaaaaatc aggtccattt aaatttgcca gttacaatag cgcattgcca
     7261 gccgatgcat ttgtggaagc ggcaaaaacg agtacaccta cagtagtaca tatcaacacg
     7321 attattaaaa caaaaaagtc agacagaaat tctaaatatg tcaatccatt ttatcagttt
     7381 tttggagatc catttggaaa ttcaccatat gatatgccac cacaacaaga ccaagaagca
     7441 tcaggctctg gcgtaataat tagcaatgat ggctatatag ttaccaataa tcacgttgta
     7501 gaaaatgcag acgaaataaa agtgagcttg tatgacaaca gagaattcag tgctaaagta
     7561 attggcaccg acccaaatac tgatttagca gtcataaaaa ttgatggaaa agatttgcag
     7621 tttttgacgt ttgccaattc tgataatgtg caagtaggac aatgggtttt ggctgttggc
     7681 aatccattta atttatcttc aaccgtgact gctggtatcg tgtctgcaaa aacgcgaaac
     7741 atcaatatat taagagaaaa agcgggtaat cttgctattg aatcatttat tcaaacagat
     7801 gcggcggtca atccaggaaa tagtggtggt gcattggttg atttgagtgg taatttgatt
     7861 ggtataaatt cagcgattgc tacgccaact ggctcgtatg ctggttattc gtttgctatt
     7921 ccagccaatt tggtcaataa agtagtcaaa gacatgattg attttggcgt tgtacaacgc
     7981 ggatttttag gtgtaagtat tcgcgagatt gacgatgaat tagcaaaaga tttaaaatta
     8041 aataaaatac aaggcgcata cattgcagaa gtaaataaag gcagtgcagc cgaagatggt
     8101 ggtgtgaaac gtggtgatgt catcatgaag gttggtgatg cggaaataaa aaactcagca
     8161 gatttacaag agcatgtagc aagatataga cctggtgaca aagtgaaatt gtcaattttt
     8221 agagatggca acatgattgc taaagatgtg gttttaaaat caaaagacaa taaaacagca
     8281 ttattaagca aagaagacgc accaaaaaca ggaaacgttc ttgataattt aggcattgcc
     8341 gttgatgagt taagcagctt agaagctaaa aaattaggtg tgagtggtgg tttaaaagta
     8401 acaaaaataa atgatggcat catcaaacaa aacaccagca tgcaagaagg ttttatcatc
     8461 ataggcataa acaatagaag cgtgactaaa aaaagcgatt taatcgattt gattaatgaa
     8521 agtaaaggca atggaatttt actacaaggg aaatatccag accaaaatgg cttaaaatac
     8581 tatgcctttg gatattaatc cttatatgtt ttttattagg ttatggtcgg atggttttta
     8641 ccatccgatt tttttattta aaaatctata ccttcgctac atggcaaaca cctattttca
     8701 attcaagcaa tttaccatac atcaagataa atgtgcaatg aaagtatgta cagattcttg
     8761 tgtatttggc gcttggattc caatacctaa aaacacacag cacatcttag atattggcag
     8821 tggaactggc ttgttgagtt taatgatggc acaaagaaca aatgcaatta ttgaaggtat
     8881 agaaattgaa aataatgctt atttacagag tgtagaaaat tttaataatt caaaatggtc
     8941 agatagatta cacatgcatc atggcaatat taaagacttc gcttttaata aaaaatttga
     9001 ttttattatt tgcaatccac cattttatga aaatgaaaaa tcatcggaaa atacagaaga
     9061 aaatatagca aaacattcca tgcatctaac attggaagaa ttaatttttg caataaaaaa
     9121 tatagtaagc gaacatggta aatttgctat actgcttcct tatttcagaa aacaagaatt
     9181 gcattctatt tgtcatcaaa ataatttttt tcttgaaaat gaaatcgaca tgcgacaaac
     9241 accaaaacat gattttttta ggtatgccgc tgtatttaca acgcaaaaaa atccaaaagt
     9301 atcacaagaa gaaatcacaa taaaagatgc acataatcat tacacagctg attttgtaaa
     9361 actaatgcaa acattttatc ttaaattata gtgtatatca tctgtatttt gagtatattt
     9421 agtaagtatt agtctatatt cttattaata acgaatattt tagtaaaaat tgaattatga
     9481 aaaagtattt acttcttatt tgtgcacttt attttaccgc atcatttgca tttgcaattg
     9541 aaccaatcag cagtacggaa cgctcaatgg ttgacacctc tttggtgcct ttttatcatg
     9601 gtgttgcttc tggcgatcca acagcgaacg ccgttgtaat atggacaaga gtaacacctg
     9661 aaactgcagg aatcgtcaat ggtacttggc ggatggcatt ggatacaaac ttcacttcta
     9721 ttgttcagtc tggcactttt tctgcagaca gcacaacgga ttactgtgta aaaatagatg
     9781 ttacaggttt acaagccaat acatggtatt tctatgagtt tactgcttta ggtagaaatt
     9841 cattaactgg cagaacgaaa actgcgccta ttagtggtgt aaaccaattg cgttttgcat
     9901 ttgtatcttg ttctaattat cctaccggat actttaatgt ttacaataga attaaagaca
     9961 gaaacgacat cgacgctgta ctgcatttag gcgattatat ttatgaatat ggtacaaatc
    10021 ctttaagtac cagaaccgac caaatgccta atcatgaaat tatacaattg gcagattatc
    10081 gtcagcgata ctcaacacat agattagatc ctgcattgcg aaaattacat caacaatatc
    10141 cgtttattac agtatgggac gaccatgaaa ctgccaataa ttcctatact aatggtgcag
    10201 acaaccatac acctggatct gaaggtcctt ggtcagtaag aaaagcggca ggacaacaag
    10261 ccaatgatga atggctacca aaacgcttgc aagatgcgca caataccaac aaaatttgga
    10321 gaaaaataag ttatggtaat ttagcagata tttttatgct ggacacaagg ttgtacgata
    10381 gaagcattat gggtggaaac acgaatgaca cctccagaca tattatagga aatgaacaaa
    10441 tggagtggtt gaaaaatgaa cttattcatt caacagcaaa atggaaaata cttggtcaac
    10501 aagtaatgat gggcaacctc actccttttg gtattatgct caataatgac caatgggacg
    10561 gttataatgc tgatagaaaa aaattatacg acattatttt aaataacaac ataaaaaatg
    10621 tcattacatt aactggcgat atacataccg cttgggcaat ggatttacca tataatacag
    10681 ctacttacaa tcctgctact ggtgctggct ctgtcgctgt tgagtttgta tgtacaagtg
    10741 ttacttcttc gtcttcacca attccattag atcctattta tggattggtc acctcattat
    10801 taccacatat caaatatgtt gatttattta aaaaagggta tggcatttta gatttaaaag
    10861 atacaattgc acaaggaaat ttttattcag tacctacagt tagcacactt aatacaggac
    10921 aagcatttga aacagcctat tacacgttag aaaacacaag atggctaaag aaaacaacaa
    10981 cagcatctgt tatgttagca ccaccatttt accaagcacc cgaaacacca cgacaaactg
    11041 tagtatcagg cattaaaaac agaagcaatg atttaatagt attaagcgct tatccaaatc
    11101 catttattga tcaaataact attcaataca atacagcatc aaatactgca ttgtctttaa
    11161 atatttacga ttttagtgga cgcttagtaa aaactcaaga cttagggttt cattcaaaag
    11221 gattatacaa taaaacgatt tccctatctg atttagcaag tggtgcatac aaaattgtat
    11281 tgagcaacaa caatgaaatt attgaaagaa caattgaaaa atattaatta tggcaatcat
    11341 aaaaaaaaca acaacaaagc ccgttaagaa aaaccttgca acaaaaaaaa gtgttactaa
    11401 aaaagtgacg cctaagaaag ctacttcatt aaaaagtgta acaaaaaaac cacaagctca
    11461 aaagcctaaa cctatagtaa aatcagttat tcaggcggaa aaaactgatc caaaaatatt
    11521 tccaatagga aaatttagtg tgcctcatga atatacagat gtggtaatca acaatctgac
    11581 acaagagcta aaatcgttgc caaaggattt aaaaaaagta gcaaaaaaaa tcaaatctga
    11641 tgcacaatta caagcatcat atagagatgg tggctggagt attgaacaaa ttatacatca
    11701 tcttgccgac agtcacatga atgcttttat tagatttaaa cttagtttga cggaaaacaa
    11761 tccagtcatt aaaccatata atcaagattt atgggcagaa acagccgaca ttcaattgcc
    11821 cataaaaata tcaataaaat tgctaaaagc attacacaca aaatgggtct gcttattaga
    11881 aaatatggat aaaaatgatt tcaaacgaac atacacacat cctgaatata aaacaacacc
    11941 tgcattacaa gaaattttgg cacaatatgc ttggcatggc aaacatcatt tagctcaaat
    12001 tgaattagca ctaaattcaa ttgaaatcga aaaagtagat gaagagaaaa agagtaaaga
    12061 aagtaaatca ataaaaccaa aatctaaacc tgcacgaaaa cattagtatt tattgacatc
    12121 ttgatgaata tttaataaga aagtacatca agcaaatgtt tccattcttc gtttatgcta
    12181 acaaaatgtt tacgaatttg tgacaaaggt gtgtagcaaa tactattttt ctctacacca
    12241 accatcacat taaaattttt attaagcaag gcatctactg ctgctgtacc taatcttgat
    12301 gcaaggattc tgtcgtttgc acttggactt ccgccacgta ataaatggcc aagtactaat
    12361 gtttttacat cataatcagg atgtaattgt ttgaatttag ctgcgatttc gagtacgcca
    12421 cctgcatcat cgccttcggc aactacaaca atataagatt ctttttgtct gctattttgt
    12481 aatttcaatg acagctcatg tatatctgtt ttcaattcag gaattaagat atcttcagca
    12541 ccagctgcca agccagcagc caatgcaata gaaccaacat cacggcccat tacttcaata
    12601 aaaaacacac gac
//
LOCUS       DAOQQV010000074        12478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1763_length_12478_cov_7.279401, whole genome shotgun sequence.
ACCESSION   DAOQQV010000074 DAOQQV010000000
VERSION     DAOQQV010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12478
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1763_length_12478_cov_7.279401"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /gene="lipB"
                     /locus_tag="PLL59_08395"
     CDS             <1..492
                     /gene="lipB"
                     /locus_tag="PLL59_08395"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HMY23867.1"
                     /translation="EALLKENNIDIFHINRGGDVTFHGPGQITGYPILDLDFFTSDIK
                     MYMRMLEEVMIRTIAHYGIEGYRIEDATGVWVNSKTDNSIKKIAAFGVKTSRWITMHG
                     FALNVDVDLNYFNFINPCGFTDKGVTSIQAELKHKNQAVSIKEIKPILLHHFATVFQA
                     EMI"
     gene            542..1576
                     /locus_tag="PLL59_08400"
     CDS             542..1576
                     /locus_tag="PLL59_08400"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMY23868.1"
                     /translation="MNELSANTEQEEDELFEHHRFVIDKGKEPVRIDKYLTDIIPSIS
                     RNRIQNATNAGSVLVNGKPVKSNYKIKPLDVIQVVLPNPVWDYTVEPENIPLDIVYED
                     DDVLLVNKKAGMVCHPGHGNFHGTLVHALLWHCKDLPQITGDTRPGLVHRLDKNTSGL
                     IIIGKTEHALTHLSKQFFDRTIKRHYIAMVWGDIEQSSGRIVCNIVRNPNNRKTFMAV
                     EEEHIGKFAATNYEVIKRYGYTTLVKCKLETGRTHQIRVHLKYIGHTLFGDYSYGGDQ
                     ILSGTIYGKYKTFIENCFALMPYHALHAQSLGFVHPRTNQEMYFEVPLPENYQKVIEK
                     WENYTKGFKL"
     gene            complement(1958..3001)
                     /locus_tag="PLL59_08405"
     CDS             complement(1958..3001)
                     /locus_tag="PLL59_08405"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMY23869.1"
                     /translation="MTNSKGYAVKHAKDNFSLWNFERREVGDKDIQIDILYCGICHSD
                     IHQVRSEWFPGFYPMVPGHEIVGRITKVGQQVTAFKVGEYAGVGCMVDSCRTCDNCNR
                     GYEQFCKTGAVYTYNALERDGKTPTYGGYANNIIVDQKFALHVSDKLPLAGVAPLLCA
                     GITTYSPIKKWKISKGHKVAVLGLGGLGHMAVKFAVAFGAEVTVLSTSASKEIDARNL
                     GAHNFCNTKDKEQVKKYNAYFDFIIDTVSAAHDYDMYLKMLATNGVQILVGIPETPIQ
                     LNAFSLLSNGRTLTASSIGGIAETQEMLDFCVAHNIVSDIEIINANYIDEAYERVIKS
                     DVKYRFVIDCKTI"
     gene            complement(3024..3452)
                     /locus_tag="PLL59_08410"
     CDS             complement(3024..3452)
                     /locus_tag="PLL59_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HMY23870.1"
                     /translation="MPIIPPNIKLVNNTKYILCAVRKKYILLTPEEKVRQYILQMLVH
                     HFQYPLKYISVEKTLIVNDLKKRYDIVVFNQQLQAKILIECKAEYIEIKEKTLQQIAT
                     YNTKLQVPYLMVTNGNSHYYFEINANASLQIDTLPAFDNL"
     gene            complement(3452..5038)
                     /gene="lepB"
                     /locus_tag="PLL59_08415"
     CDS             complement(3452..5038)
                     /gene="lepB"
                     /locus_tag="PLL59_08415"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMY23871.1"
                     /translation="MLSLVYAYLLHYLLHLPCFGLYKKAGKNPMHVFIPLVQDFTLLE
                     IIGRKRNHAYWGLVPYINLLFGLTWTTDICNSFGKRSLWQHVLGIFFGYIYFPIIGFD
                     TKTTYEGPSHINDKLPITAAKDNKTKKINSTPKVKRSFAREWADAIIFAVVAATIIRT
                     FVIEAYKIPTTSMEGSLLAGDFLFVSKLNYGARTPITPIAFPFAHHTMPFNLGQAYSK
                     AIQLPYYRLPGFETVKRGDVVVFNYPGDALEYPERPIDKRENYVKRCVAIAGDTMQIK
                     NRQLYINNIAQAHYKKMQLAYNIITNGNKITASDIEAADVRWYNQFMELRMDENGIPV
                     RPVDELFSYFENYLQIKDTIPVVLNDEQKNILLKNKNVKSINPISETEFNAVIGEGYL
                     EALFPRNRNMFQWNLDNFGPLIIPKKGWTVTLNKANIILYGFTIRAYEKQNIELNENG
                     EAIIDGQKAATYTFKQDYYWMMGDNRHNSADSRYWGFVPEDHIVGKPLFVWLSKDNFH
                     SFLESIHWNRMFRSVSDLCK"
     gene            complement(5097..5843)
                     /gene="dapB"
                     /locus_tag="PLL59_08420"
     CDS             complement(5097..5843)
                     /gene="dapB"
                     /locus_tag="PLL59_08420"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HMY23872.1"
                     /translation="MKIALIGYGKMGKAIHEIAKEKNEKAGKLVYDISLIVDIDNRAT
                     ISKEELQQVDVAIEFTSPHTAVENIHWCFDADVPVVVGSTGWTAKLAEIQAYAKENNK
                     AFLFAPNFSIGVNIFFELNRYLAKIMNEHTEYDVIMEEIHHTEKKDAPSGTALHAALD
                     ILKGLERKSDWKNENSDDSSTLSIISKREDLVPGTHQITYESEIDKIDIIHTAHNRKG
                     FAGGALMAAAWLHNKKGIYSMEDVLGFNKH"
     gene            complement(5882..6643)
                     /locus_tag="PLL59_08425"
     CDS             complement(5882..6643)
                     /locus_tag="PLL59_08425"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HMY23873.1"
                     /translation="MISKKDESTMKFIFFILLIFLISFSFAQNAPIDSIGIPAQSMLK
                     DTIISKDNTEKIGNKFDSISENFALQYKQQNAKFIHEDSIAQAQTSNKKNKKIGKTKK
                     VDTTHYSPKKAAIWGAAFPGLGQIYNKRYWKLPIVYGILGTVIYFVAANGKSLREFNG
                     YIRNVYDSIPNPAPYTNISLAQIESFRNTYRRNVQIASFGTLFAWGLSIVDAVVDAHL
                     RSFDISDKLTFKVKPQLNYSNFSYYAGIGVHLKIK"
     gene            complement(6668..7570)
                     /locus_tag="PLL59_08430"
     CDS             complement(6668..7570)
                     /locus_tag="PLL59_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMY23874.1"
                     /translation="MSKKKDALGKGIRALLSDIDESSDILKISATDEDSIINTVPKIK
                     LEQIEVNPFQPRADFNKEALEELSASIKIHGVIQPITVRKISDKKYQLISGERRLRAS
                     KMAGMEEIPAYVRTANDQEVVEMALIENIQREDLNSIEVALTYQRLIDECDLTHENLG
                     DRLGKDRSTVTNYLRLLKLPPEIQKALRDKILSMGHARAIISVPEVDKQLYVLKEVNS
                     KGLSVRKTEELVRLLSSGNTAKKTEKKEVALPASYKNVQTKLMDLFETKVKIKKTAAD
                     KGEIVIPFYSVADLNRLLDIIEKD"
     gene            complement(7578..8396)
                     /locus_tag="PLL59_08435"
     CDS             complement(7578..8396)
                     /locus_tag="PLL59_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMY23875.1"
                     /translation="MGKVISIANQKGGVGKTTTAINLAASLAILEQKTLIIDADPQAN
                     STSGCGFDPRNVKVSLYECLIDSVKAKDIILETETPNLFLLPAHLDLVGAEIELINHP
                     NREQIMREMLQDLKKTYDFIIIDCSPSLGLITVNALTASDSVLVPVQCEYFALEGLGK
                     LLNTIKIVQSRLNKELAIEGILLTMYDQRLNLSNQVVAEVKRRFENSVFNTIIHRNTR
                     LGEAQSLRQPIIMYDAESKGAVNYLNLAREIMQNNGMTNIPSEEKFIEVDENLN"
     gene            complement(8401..9081)
                     /locus_tag="PLL59_08440"
     CDS             complement(8401..9081)
                     /locus_tag="PLL59_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMY23876.1"
                     /translation="MKFTYYGHSCFLVETNGKKILFDPFITANELAKHIDIHTIECDY
                     IAISHGHFDHIADAVSIATRTNATIICAYEIYEWLSKQGLKNFRPMNTGGKWKAEFGS
                     VKCVAAVHSSNLPDGTYGASPMGFVFNTQDGDFYYSGDTALTLDMQLIPRWANLKFAV
                     LPIGDNFTMDISDAIAAADFVQCNKIIGVHYDTFGFIVIDHVAAKQAFAQAGKELILV
                     KIGETIEL"
     gene            complement(9254..10849)
                     /locus_tag="PLL59_08445"
     CDS             complement(9254..10849)
                     /locus_tag="PLL59_08445"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMY23877.1"
                     /translation="MAKFIFVTGGVTSSLGKGILAASLAKLLQARGLRVTIQKFDPYI
                     NIDPGTMNPYEHGECYVTEDGAETDLDLGHYERFLGVYTSQANNVTTGKIYQEVINRE
                     RRGDYLGKTVQVIPHITDEIKRRMLLLGTSGQYDIILTEIGGTVGDIESLPFIEAIRQ
                     LKWKLPKEDYLVIHLTLVPYLASAKELKTKPTQHSVKELQSYGVNPHIIVCRTEHPLG
                     NDRKIKIAEFCNVDYDSVIEALDAETIYDVPLYLKKEKLDQIVLNKFGYNDTPDTDLV
                     QWNNFLQRLKFPKKEITIGLIGKYIELKDAYISIYESFIHAGAQNECKVNVERIHSEE
                     INKNNVKSRLSHLDGIFVAPGFGQRGIDGKIVTIQYARENNIPFFGVCLGMQMAVIEF
                     AKNILQLEDANSTEMKQNCKNPVIDMMQEQKTIVNMGGTMRLGAYNCHLYENTLAHKI
                     YGETTITERHRHRYEFNSKYLQQYTDNGMIASGINPDSNLVEIVEIPSHKFFIGVQFH
                     PELKSTILKPHPLFSEFVKACLS"
     gene            complement(10972..11640)
                     /locus_tag="PLL59_08450"
     CDS             complement(10972..11640)
                     /locus_tag="PLL59_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23878.1"
                     /translation="MKEAEENQKKLQRNNITMIIKSIKIQVLLLLIVVLIFTACELFK
                     QNEEEKIEIPEGVTQQQLQNERYLFKRNKSYNNKFLHQTKTDSAVITIYDIIHYQIKD
                     KESSIGNDAVFYIFDISVDNPTDKPFKIADFTNSCFLSNEDENYHYSNVGMALKMYYL
                     QSDSAQIDMEYTKRFYTQTMPEKEFYRTKLFAFEVSTEDKHPLFLHYQIGAQKFQYQV
                     RKEY"
     gene            complement(11597..>12478)
                     /locus_tag="PLL59_08455"
     CDS             complement(11597..>12478)
                     /locus_tag="PLL59_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMY23879.1"
                     /translation="DFTIQSEYFKHSQFAALKLKDAICDRFRKLNDDKRPDINTDRPD
                     VLFNLHIHRNQVTISLDSSGTSLHQRGNRVEQTIAPINEVLAAGLILLSKWDKASHFV
                     DAMCGSGTIVIEAASMAINKAPNIDRNYFCFKNWKSYDKSLYNDICTELSLQTKSFPF
                     KIIGSDNNKKAVFVAQQNVKKAGFNHIIEIREVSIQKMIPPLDRGMLIINPPYGERMQ
                     TFETDRLYKQIGTAFKEHFTGYTCAIITSDTVALKNIGLKPYKKYSLINGKLDCLYCL
                     YEMYEGSRRKPKEIAAE"
BASE COUNT         3850 a         2120 c         2025 g         4483 t
ORIGIN      
        1 gaagcattac tcaaagaaaa taatatcgat atatttcaca tcaaccgcgg tggtgatgtt
       61 acctttcatg gcccaggaca aataacaggt tatccaattt tagatttaga ttttttcact
      121 tctgatatca aaatgtatat gcgtatgttg gaagaagtaa tgataagaac gattgcgcat
      181 tatggtattg aaggctatag aattgaagat gctactggcg tttgggtaaa ttcaaaaaca
      241 gataattcaa taaagaaaat agctgcattc ggtgtaaaga catcgcgatg gataactatg
      301 catggttttg cattaaatgt tgatgtagat ttaaactatt tcaattttat caatccatgt
      361 ggctttacag acaaaggcgt tacgtctatt caagccgaat taaaacataa aaatcaagct
      421 gtctctatta aagaaattaa accgatattg cttcatcatt ttgctacagt atttcaggca
      481 gagatgatat aactattttt tctgtttgtg tgctttgctc gaaaaaatgt atttttgtgc
      541 cgtgaatgaa ttatcagcaa atacagaaca agaagaagat gagttgtttg aacaccatcg
      601 ttttgtaata gataaaggca aagaacccgt ccgaattgat aaatatctaa cagacattat
      661 tccatctatt tctagaaata gaattcaaaa tgctaccaat gctggtagtg ttttggtaaa
      721 tggaaaacct gtaaaatcaa actataaaat aaaaccatta gatgtaattc aagtggtatt
      781 gcctaatcca gtttgggatt atacggttga accagaaaat attccattag atatcgtata
      841 tgaagatgac gatgttttat tggtcaataa aaaagccggt atggtttgtc atcctggtca
      901 tggtaatttt catggtactt tggtgcatgc tttactttgg cactgtaaag acttacctca
      961 aattactggt gatacacggc cgggtttagt tcatcgtttg gataaaaata cttctggatt
     1021 aattattata ggaaaaacag aacatgcact cacacatctt tcaaaacagt tttttgatag
     1081 aactattaaa cgccattata tagcaatggt ttggggcgac atcgagcagt cgagtggtcg
     1141 tattgtttgt aatattgtta ggaatcctaa caatagaaaa acgtttatgg cagtagaaga
     1201 ggagcatatt ggcaaatttg ctgcaacaaa ttatgaagtc ataaaacgtt atggctatac
     1261 tactttagta aaatgcaaat tagaaacagg aagaacacat caaattcgtg tgcatttaaa
     1321 atacattggc cacactttat ttggtgatta tagttatggt ggcgatcaga ttttatcagg
     1381 cacgatttat ggaaaatata aaactttcat agaaaattgt tttgcactaa tgccttatca
     1441 tgccttacat gctcaaagtt taggattcgt gcatcctcgc accaaccaag aaatgtactt
     1501 tgaagttcca ttacctgaaa actatcaaaa agtaatcgaa aaatgggaaa attacactaa
     1561 aggctttaag ttataagcca tattttttct gttttttgta gtttattatt ctattttatt
     1621 ttcatttttg tgtataaagt gttctttatg aatgagcaat tttgtggaca agtatatgga
     1681 ttttatggtg cataagtttt ctattacttt acttgacaat tattaagttc taatttacat
     1741 ttacaatgag ttcaatgaaa aactgagtaa cgagagaagt aaaagaaata aacttggcaa
     1801 aacaagggaa actttttgct aatgaataat gtcaaagtta tttaattaga cgtgaaaaga
     1861 aattcaagcg acaaaagtta cgcagaaata aataacctca aaaagttatg aaactgcaca
     1921 atttggaaga caaggtttgt aaaaactttg tcttttttta tatcgtttta caatcaatca
     1981 caaatcgata ttttacatca ctttttatca cgcgctcgta agcttcatcg atataattag
     2041 cattaataat ttcaatatca gaaacgatat tatgtgcaac acaaaaatcc aacatttctt
     2101 gtgtttctgc gatgccacca attgatgaag cagttaatgt tctaccgttt gacaataatg
     2161 aaaatgcatt taattgtatt ggtgtttcag gaattccgac aagaatttga acaccattgg
     2221 ttgctagcat tttcaaatac atatcgtagt cgtgtgctgc tgaaaccgta tcgataataa
     2281 aatcaaaata ggcattatat tttttcactt gctctttatc ctttgtgtta caaaaattat
     2341 gagcacctaa atttctagca tctatttctt tgcttgcaga tgtacttaac accgtaactt
     2401 ctgcaccaaa tgccacagca aatttcacag ccatatgtcc aagtccacct aaacctaaaa
     2461 ctgccacttt gtgcccttta ctaattttcc attttttaat cggagaatac gtagtaatac
     2521 cagcacaaag caaaggtgca acacctgcta atggcaattt atctgataca tgtaatgcaa
     2581 atttttggtc aacaattata ttattggcat aaccaccata agtaggcgtt tttccatctc
     2641 tttctaatgc attgtaagta tagacagcac cagttttaca gaactgctca taacctcgat
     2701 tgcaattatc acaggttcta caggaatcga ccatacagcc aactcctgca tattcgccta
     2761 ctttaaaagc tgtaacttgt tgtcctactt ttgtaattcg tccaacaatc tcgtgtccag
     2821 gtaccattgg ataaaaacca ggaaaccatt cactgcgcac ttgatgtata tcagaatggc
     2881 atataccaca atataaaata tcgatttgta tgtctttgtc gccaacttct ctgcgttcaa
     2941 aattccaaag cgagaaattg tctttcgcat gttttactgc gtatcctttg ctatttgtca
     3001 tgacaataaa gttataaata ttattaaagg ttgtcaaatg ctggcaatgt gtctatttgc
     3061 agcgatgcat tggcatttat ttcaaaataa taatgcgaat tgccatttgt aaccattagg
     3121 taaggcacct gtaatttggt attgtatgtt gcaatttgct gaagtgtttt ttcttttatt
     3181 tcgatgtatt ccgctttaca ttctatgaga attttcgctt gcaattgctg attaaataca
     3241 actatgtcat atcgtttttt caaatcattt actatcaatg ttttttctac agaaatatat
     3301 tttaaaggat attgaaaatg atgcacgagc atttgtaaaa tatactgtcg cactttttct
     3361 tctggtgtta atagtatgta ttttttccga acagcacaaa gtatgtattt tgtattgttg
     3421 accagtttta tatttggcgg aattattggc attacttaca taagtcagat acacttctaa
     3481 acattctgtt ccaatgtata gattctaaga atgaatggaa attgtcttta gacaaccaaa
     3541 caaataaagg ttttcctaca atatgatctt caggaacaaa accccaatat cttgagtcag
     3601 cggagttgtg tctgttgtcg cccatcatcc aataataatc ttgtttgaaa gtataagttg
     3661 cagctttttg tccatcgatg atggcttcgc cattttcatt tagttctatg ttttgttttt
     3721 cataagcacg aatggtaaag ccatacaata tgatatttgc tttgttgagc gtaactgtcc
     3781 aacctttttt aggaataata agtggtccga aattatctaa attccattga aacatatttc
     3841 tgtttcttgg aaataatgct tctaaatagc cttcgccgat tacagcatta aattcagttt
     3901 cagaaattgg atttattgat tttacatttt tatttttcag taaaatattt ttttgttcat
     3961 cgtttagtac aactggaatg gtatctttta tttgaagata attttcaaaa tatgaaaaaa
     4021 gctcatctac aggtcgaaca ggaataccgt tttcgtccat tcgtaattcc ataaattgat
     4081 tgtaccaacg cacatcagca gcctcgatat ctgaggctgt aattttattt ccattggtga
     4141 tgatgttata tgctaattgc atttttttat aatgcgcttg cgcaatattg ttgatgtata
     4201 attgtcgatt ttttatttgc atggtgtcac cagcaatagc cacacatctt tttacataat
     4261 tttctctctt gtcgattggt ctttccggat attctagcgc atcgccagga taattaaata
     4321 caaccacatc accgcgctta acagtttcaa aaccaggcaa acgatagtag ggaagttgta
     4381 ttgcttttga atatgcttgc cctaaattaa atggcatcgt atgatgtgca aatggaaaag
     4441 ctattggcgt aatgggtgta cgagcaccat aatttaattt acttacaaat aaaaaatcgc
     4501 cagcaagtag tgagccttcc attgatgtag ttggaatttt gtatgcttca atgacaaatg
     4561 ttcgaataat ggttgcagca acaacagcga aaataatagc atctgcccat tctcttgcaa
     4621 atgaacgctt aacttttggt gtgctgttta tttttttcgt tttattatct tttgctgctg
     4681 taattggcaa tttatcattg atatgacttg gaccttcgta tgttgttttt gtatcaaatc
     4741 caataatagg aaaatagatg tagccaaaga aaatacctaa aacatgttgc cataaacttc
     4801 gtttgccaaa tgaattacaa atatctgtag tccatgttaa tccaaacaac aagttaatgt
     4861 agggtaccaa accccaatag gcatgatttc tttttctgcc tattatttcc aataatgtaa
     4921 aatcttgcac caaaggaata aaaacatgca ttggattttt ccccgctttt ttatacaatc
     4981 caaagcaagg tagatgtaac aaataatgta gtaaatacgc gtaaactaag ctgagcataa
     5041 ttgatttatt ttttgatatt cgttattgaa atggctagat tattacaatt ttatgcttaa
     5101 tgtttgttaa accctaaaac atcttccatt gaatatattc cttttttatt gtgcaaccac
     5161 gcagcagcca tcaatgcacc tccagcaaat cctttgcgat tatgtgcagt atgaatgata
     5221 tctattttgt cgatttctga ttcataagta atttgatgtg taccaggcac aaggtcttct
     5281 cttttcgaga taattgataa tgttgaactg tcatcactat tttcattttt ccaatcagat
     5341 tttctttcta atccttttaa gatatcaaga gcggcatgta gtgctgtgcc actgggtgca
     5401 tctttctttt cagtgtgatg tatttcttcc atgatgacat catattcagt atgttcattc
     5461 ataatttttg ccaaatatct attcaattca aaaaaaatat tgacaccaat actaaagttt
     5521 ggcgcaaata aaaatgcctt attattttct tttgcataag cttgaatttc tgctaattta
     5581 gcagtccagc cggtgcttcc tacaacaaca ggcacatcag catcgaagca ccaatgtatg
     5641 ttttctacag ctgtatgagg cgaggtgaat tctatagcaa catctacttg ttgtaattct
     5701 tctttagaaa ttgtagctct gttatctata tctacaataa gagagatgtc atacactaat
     5761 ttgcctgctt tttcattttt ttctttagca atttcatgta ttgcttttcc catttttcca
     5821 taacctatta atgcaatttt catcttattc aatatttata atgttgttaa tgtgtattga
     5881 tttattttat tttcaaatgt actccaattc cagcgtaata gctaaaattg gaataattta
     5941 attgcggttt aactttaaaa gtaagtttat cagaaatatc aaatgagcgc aaatgtgcat
     6001 ctactactgc atctacaata gacaagcccc aagcgaaaag cgttccaaat gatgcaattt
     6061 gaacattgcg ccgataagta tttctgaatg attcaatttg tgctaatgaa atatttgtat
     6121 aaggcgctgg attgggaata ctatcatata catttcttat gtagccatta aattctctta
     6181 aactttttcc atttgctgca acaaaataga ttacagtgcc gagtatgcca taaacgatag
     6241 gcagtttcca atatcgctta ttatatattt gacctaaacc tggaaatgct gcgccccaaa
     6301 tggcggcttt cttaggcgaa taatgtgttg tatctacttt tttagtttta cctatttttt
     6361 tatttttttt gttggaggtt tgtgcttgag caatagaatc ttcgtgtata aattttgcat
     6421 tttgttgttt gtattgcaaa gcaaaatttt cagaaatgga atcaaattta ttaccaattt
     6481 tttctgtatt atctttcgat attatagtat ccttaagcat tgattgagca ggaattccta
     6541 tgctgtcaat aggtgcattt tgagcaaagg aaaatgaaat taagaaaatc aataaaataa
     6601 aaaagatgaa tttcatagta gattcatctt tcttacttat catatttata ttgacttttc
     6661 taagcattta gtctttctca atgatgtcta aaagtctgtt taagtcagca acagaataaa
     6721 atggaataac gatttcgcct ttgtctgctg ccgtcttttt gatttttact ttagtttcaa
     6781 aaagatccat caattttgtt tgcacatttt tataggaagc aggcaatgcg acttcttttt
     6841 tttcagtttt tttagctgtg tttccagaag ataataagcg cactaattct tctgtttttc
     6901 gaacagacaa acctttacta ttcacttctt ttagaacata taattgtttg tccacttctg
     6961 gcacagatat aattgcacga gcatgaccca ttgacaatat cttatcgcgc aatgcttttt
     7021 gaatttcagg tggcaatttt aataaacgca aatagttggt aacagttgaa cgatctttgc
     7081 ctaatctgtc gcctaaattt tcatgagtta aatcgcactc atcgattaag cgttgatagg
     7141 tgagtgccac ttcgatagaa tttaagtctt cacgttgaat attttcaatc aacgccattt
     7201 ctactacttc ttggtcgtta gctgttctga cataagccgg aatttcttcc atacctgcca
     7261 ttttcgaagc gcgcaatctt cgttcgcccg aaattagttg atatttttta tcactaattt
     7321 ttcgaactgt tattggttga attacaccat ggattttaat tgaagcagaa agttcttcta
     7381 atgcttcttt attaaaatcg gcgcgtggtt gaaaaggatt tacttctatt tgttccaatt
     7441 tgatttttgg aacggtattg ataattgaat cttcgtctgt tgcacttatt ttgagtatat
     7501 cgctactttc gtcgatatca gataataaag cacgaattcc ttttcctaat gcatcttttt
     7561 tcttactcat taacatctta atttaaattt tcatctacct caataaattt ttcttccgaa
     7621 ggaatatttg tcattccatt attttgcata atttctctag ctaagtttaa atagtttaca
     7681 gcacctttgc tttccgcatc atacatgatg ataggctgtc gcaaactttg tgcttcgcct
     7741 aaacgtgtat ttctatgaat gatagtattg aaaacgctat tttcaaatcg gcgttttact
     7801 tctgctacta cttgatttga taagttcaat cgttgatcat acattgttag caaaatacct
     7861 tcaattgcca attctttatt taaacgagat tgtacaattt tgatagtgtt taataatttc
     7921 cctaaacctt ctaatgcgaa atactcacac tgcacaggca ctaaaacgga gtcagatgcg
     7981 gtcaatgcat tcacagttat taaacctaat gaaggagagc agtcaatgat gataaaatcg
     8041 tatgtttttt ttaaatcttg caacatctca cgcataattt gctctctatt gggatggttg
     8101 ataagttcta tttcggcacc tactaaatct aaatgtgctg gtaacaaaaa caaattaggt
     8161 gtttctgttt ccaagatgat atcttttgct tttacgctat cgattaaaca ttcatacaaa
     8221 cttactttca cgttgcgtgg atcgaaacca caaccactcg tgctatttgc ctgtggatca
     8281 gcatctatga ttaatgtttt ttgttctaaa attgctaagc ttgctgctaa atttatagct
     8341 gttgttgtct ttcctacacc gcctttttga ttggcaatac tgattacttt tcccatattc
     8401 ttataattct atcgtttcac ctatttttac taatattaat tctttgccag cttgtgcaaa
     8461 tgcttgtttg gctgctacat ggtcaatgac tataaatcca aatgtgtcat agtgtacgcc
     8521 aattatttta ttacattgta caaaatcagc agcagcaatg gcatcgctga tatccatagt
     8581 gaaattatcg ccaattggta atactgcaaa ttttagattt gcccaacgtg gaataagctg
     8641 catgtctaag gttaatgcag tatcgccact ataatagaag tcgccgtctt gtgtgttgaa
     8701 tacaaaaccc atcggactgg caccatatgt gccatcaggt aagtttgatg aatgcacagc
     8761 agccacacat tttacgctac caaattctgc cttccatttg ccacccgtat tcatcggtct
     8821 aaaatttttc aaaccttgtt ttgatagcca ttcataaatt tcataagcac aaataattgt
     8881 agcatttgtt cttgtagcaa tagaaacagc atcggcaatg tggtcgaagt ggccatgcga
     8941 aatggctata taatcacatt caatcgtatg tatgtctata tgtttagcta attcattggc
     9001 cgtaataaat ggatcaaaca agattttttt tccatttgtt tctaccaaaa aacaagaatg
     9061 tccgtaatag gtaaatttca tgtatataaa taaaataata ttttgaatga tgaaacaata
     9121 ttgttccaca gttcaaaaat aacctattaa atgcatcatt tttaatctac acgagaagaa
     9181 ttacaaacaa tttttgtgga taaaatttaa tcaattgtgt atgaatgaat ttgcgataaa
     9241 aatttgaaga aatttaactc aaacatgctt taacaaattc actaaacaat ggatgcggtt
     9301 tcaaaattgt gctttttagt tctggatgaa attgcacacc aataaaaaac ttatgggaag
     9361 gaatttcgac tatttcaacc aaatttgaat caggattaat gccgctggct atcatgccgt
     9421 tgtcagtata ttgttgtaaa tatttgctat taaattcgta acgatgacga tgacgttcgg
     9481 tgatggtcgt ttcgccgtat attttatgcg ctaaagtgtt ttcgtaaagg tgacaattat
     9541 aagcacctaa tcgcattgtt ccgcccatat ttacgatggt tttctgctct tgcatcatgt
     9601 ctataactgg atttttgcaa ttctgcttca tttctgtcga attggcatct tcgagctgta
     9661 aaatattttt agcaaactcg attaccgcca tttgcatacc taagcacaca ccaaagaatg
     9721 gaatattgtt ctcacgtgca tactgaatgg ttactatttt tccatcgatg ccacgttggc
     9781 caaagccagg tgcaacaaaa atgccatcta aatgactgag tctagatttg acattattct
     9841 tattgatttc ttctgaatgg atacgttcta catttacttt acattcattt tgtgcacctg
     9901 catgaataaa tgattcatat attgaaatat aagcatcttt taattcgata tattttccga
     9961 ttaaaccaat tgtaatctct tttttaggga atttaagacg ttgtaagaaa ttattccatt
    10021 gaactaaatc tgtatctggc gtatcattat agccaaattt attgagaaca atttgatcta
    10081 atttttcctt tttcaagtat aatggaacat cataaatggt ttctgcatct aatgcttcaa
    10141 tcacagaatc ataatcgacg ttacaaaatt cagcaatttt aatttttcga tcattaccta
    10201 atggatgttc tgttctgcac acgataatat gcggatttac accataagat tgtagttctt
    10261 ttacactgtg ttgtgttggt ttggttttta attcttttgc agatgctaag taaggtacca
    10321 atgtcaaatg aatgaccaag taatcttctt ttggcagttt ccattttaat tgtcgaatgg
    10381 cttcgataaa tggcaatgat tcgatatcgc caacggtgcc gccaatttct gtcaagatga
    10441 tgtcgtattg acctgaagtt cctaatagca acatgcgtct ttttatttcg tcggtgatgt
    10501 gcggaatcac ttgaactgtt ttgccaaggt aatcgcctcg acgttcgcgg ttgataactt
    10561 cttgataaat tttgccagtt gtaacgttgt tagcttgtga ggtataaaca cctaagaatc
    10621 gctcgtaatg accgaggtct aagtcggtct ctgcgccgtc ttctgttaca tagcattcac
    10681 catgttcata cgggttcatg gtgcctggat caatattgat ataagggtca aatttttgaa
    10741 tagttacacg caatccacgt gcttgcaata atttggctaa tgaagccgct aaaatacctt
    10801 ttcctaatga agatgttacg ccaccggtaa cgaaaataaa ctttgccata acagttgagt
    10861 ggttatggac tgtaaagtta aatcataaat tcattattgc aaattaaatt atttgatatg
    10921 acaataaaac gacgtattta aagcgtgttt atcggtttat ttgtcattaa tttaatattc
    10981 ctttcttact tgatactgga atttttgagc accaatttga taatgcagga atagtggatg
    11041 tttgtcttca gtgcttactt caaaagcgaa aagcttggtt ctgtagaatt ctttttctgg
    11101 cattgtttgc gtataaaaac gctttgtgta ttccatatct atctgtgcgg aatctgattg
    11161 taaataatac atttttaatg ccatgccaac attactataa tgatagtttt catcttcatt
    11221 agaaagaaaa caactatttg taaaatcagc aattttaaaa ggtttatctg ttggattatc
    11281 aacagaaata tcaaaaatat aaaacacagc atcattacca atagaagact ctttgtcttt
    11341 tatttggtag tgaataatat cataaatagt aatcacagcg ctgtctgttt tggtttgatg
    11401 caaaaactta ttattgtatg atttatttct tttaaataag tagcgttcgt tttgtaattg
    11461 ttgctgcgtt acaccttctg gaatttctat tttctcttct tcattttgtt taaataattc
    11521 acaagcagta aaaattagaa ctactattaa aagtaacagc acttgtattt ttatggattt
    11581 tataatcata gtaatgttat tccgctgcaa tttcttttgg ttttcttctg cttccttcat
    11641 acatttcata taaacagtat aggcaatcta atttaccatt tattagcgaa tattttttat
    11701 atggtttcaa accaatattt ttgagtgcaa cagtatcgct ggtgatgatg gcacaagtat
    11761 agcctgtaaa atgttcttta aatgctgttc caatttgttt atatagccta tccgtttcaa
    11821 aggtttgcat acgctctcca tacggtggat tgatgataag catgcctctg tctaatggtg
    11881 gaatcatctt ttgaatgctc acttcccgaa tttcaataat atggttaaat ccagcttttt
    11941 ttacattctg ttgtgcaacg aaaacagcct ttttattatt gtcagaacct ataattttaa
    12001 aaggaaatga ttttgtttgc aaagataatt cagtacaaat gtcattatat aaacttttgt
    12061 cgtagctttt ccaattttta aaacagaaat aattcctatc tatatttggc gctttattga
    12121 tagccatgct tgcagcttca attactatag tgccagagcc acacatcgca tctacaaaat
    12181 gtgatgcttt gtcccatttg ctcagtaaaa taaggccagc tgctaacact tcattaatag
    12241 gagcaatggt ttgctccact ctattgccac gttgatgcaa agaagttcct gagctatcta
    12301 aagaaatagt cacttgattt ctgtgaatat gcaagttaaa caagacatca ggtctatctg
    12361 tattaatatc agggcgtttg tcgtcgttta acttccgaaa acggtcgcat atagcatctt
    12421 ttaatttaag tgcagcaaac tgagaatgtt tgaaatattc tgattgtata gtaaaatc
//
LOCUS       DAOQQV010000075        12398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1777_length_12398_cov_8.056550, whole genome shotgun sequence.
ACCESSION   DAOQQV010000075 DAOQQV010000000
VERSION     DAOQQV010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1777_length_12398_cov_8.056550"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..7265
                     /gene="sprA"
                     /locus_tag="PLL59_08460"
     CDS             42..7265
                     /gene="sprA"
                     /locus_tag="PLL59_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HMY23880.1"
                     /translation="MDLRDPKIIDKKVEYDPQTQKYVLYEKIGDTYYKTPTYMTFDEY
                     IEYTARESEQGYFLQRSRAIDLAERKSKQPFLYQGPELFNRFLGGTKIEIKPQGNVDV
                     TIGVNSQRVENPVLLQNQRRNINFDFDMNIQLGLQASIGDKMKLGINYNTKSGFAFEN
                     QLKLGYKGKEDDIIQALEFGNVSMPLRSALIKGPQNLFGVKTQLKFGRLTITNVLAQQ
                     KSKTENVRIENGAQTKKFAIKADEYEDNRHFFIAQYFRDTYEQSLSKLPFVAAQTVIN
                     RIEVWVTNKTRQTEEVREVVAFQDLGETSKIFNTTAVTSNGGTYPDNKSNTLYNTITR
                     SSNSLDFRDPSRCISFLEGKGLQAVDDFEKVSARKLATTEYYFHPQLGYISLNQQLRP
                     DEVLGVAIQYTVNGEVFQIGEFATDLPPLADTTKTKDQILALKMLKATSVRTVEPIWD
                     LMMKNVYSLSAYNVSPEQFFFDIYYIDPGGGQKRYLPDAGPITGKQLLQVLNLDNVNN
                     QLDPQNDGRFDFIPNVTITPRNGKIYFPVLEPFGSSLANAIGDPTIANKYVYRYLYDT
                     TKFAAQQFPEFNRFILKGSYRGTDNSTFRLPGAFNLPQGSVTVSAGGNQLKENIDYTV
                     NYGIGEIVIVNQGVLNSGIPIDIKFENNVLFGVVNKSLFGTRLDYAVNKNFTIGLTHM
                     RLAEKPFTQKVNFNDDPVKNNIIGLDINYATESKGLTRAFNKITAQDITTPSKVSVAA
                     EAAYFKPGHAKAINIDNAGTVYVDDFEGASTNFDLKGSFLTWSLASAPKGMPNIFGVE
                     KFPEARISDSLVYGFNRAKLAWYSVDPVFQSNTNSNPLSSALKQQTLSSIYTRQFYEQ
                     DIFINRQNTTITNPPLYTLDLSYFPEERGPYNYETTPTVYSKGVNASGKLNNPETRWA
                     GIMRTIEGTSDFEAANIEFVQFWVLDPFKFPGANRKGNLYVQLGNLSEDILKDSRKQY
                     ENGLPRPNGGTTVDTSIWGVTPTIQNALTNSFDADPDVIKKQDVGLDGLDDDGERSYY
                     ANFISNLSSVVANPTNLAKLNNDPSGDNYAFNRDNNVFTENDGVITRYKDFSNAQGNS
                     SNNLNNSNINGNAKTQPDNEDLNNDNTLNETEEYFQYRVAFDDNFLANSPYIVSKQSV
                     PFNGNDSAYWYQVKIPIKEFESRVGNISDFRSIRFVRLIMSDFTDSVTMRFAQFGLVR
                     NQWRKYDLSLANPGEQLPGDESSNTEFNVTSVSVEENASRSPIPYAIPPGIIREQNIS
                     GYNNALQNEQAMSLQVCELQDGDARAVFKTTNLDLRNYKRIKLFSHAENFPGDRGSSF
                     PIKDNDISLFMRIGSDFTDNYYEYELPLKVTQPGNYNPESELDKQLIWPDSNQLNILL
                     DSLTTVKQIRNQSNASLLLPYDVTFANGSKISIKGNPDLGVASVFMLGLKNPKRIIGE
                     NENTDDGLAKCAEVWVNELRMSGFDERSGWAALGRVDVQLGNLGNVIMAGNIHTIGFG
                     DLEQRLNQRYRDNYYQYDIAANLQLGNLLPEKAGLKIPVYTQFSQSISTPQYDPYELD
                     VELKDKFNTINADGNLTKDRKKELIDSTKRVAQDVTTIKSINVTNLQKVRTNPEKEPR
                     VYDVENFNFTYAYTQTDRRNPIIETEVIKKHRGSLGWNFSPKPLVWQPFKKIKNNNIH
                     LKPVKEFNLNLKPNSVAFRTDINRQYAYQKIRNISGDDLEIHPTFDKYFTWDRFWSIK
                     YNLTKSINFDFTATNKARIDEPAGALDTKEKRDTVRKNFWRFGRNIDYSHVFNASYNL
                     PFNLFPSLDWINVRTRYGATYSWLAAPLALTSLGNTIRNSQDYQVTADVNFKNLYQKS
                     KFLKPYTLPDIKKTKQEYADAYAKYKDLDAAAKTKIIKKKEDIEKKINDIELAEKDTS
                     KTKEDIKKLVDEKKQLKNDLRKLKLEKRQLLEPANPALDYVMRPLMMLQRASFSYDVK
                     QTTVLPGFMPSPVLFGQNFKQHAPGAGFLFGAQKDTNWLNAIAAKDWMSRDTTINYQF
                     IQTKQQSMNLKVSLEPYRDFRIDITLQKTRGETYSEFFKTTTANGAFKHLTPQTNGNM
                     SMSFMMLKTVFSKVDKNNFSNAFYKFQELRELYSQKLGSLNPNSDTIYINDSIKLPNF
                     REGYGPFAQEVLVPSLIAAYTGKNPDNVRLNPLKTLPLPNWRLSYNGFAKTKWGKKLF
                     TSFNITHGYSSTFSVGSYLTNLNYINTPGYFNEDLYFVPQEMDSLNGNYYSYYLIPQV
                     TITEQLSPLLGIEISWKNSLITNFEFKKSRTLGLSLLDYRLVETRSTEYVAALGYKLA
                     KFKIPFKIRGKRITLNNDINLRADFSYRDDKTVNYRLDQNIAEPTRGQKTISLSTTID
                     YIVNNKLNVRIFYDFRRTTPATLASYPTKTHRGGITFRFSLTP"
     gene            7351..7728
                     /gene="gcvH"
                     /locus_tag="PLL59_08465"
     CDS             7351..7728
                     /gene="gcvH"
                     /locus_tag="PLL59_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMY23881.1"
                     /translation="MNIPAELKYSKEHEWLKVDGDTATIGITAFAQGELGDIIYVDID
                     CVGDNVEKNAIFGTVEAVKTVSDLYMPVSGEVLEFNEALKDKPDLVNSSPYGDGWMIK
                     IRLSNPAEIDELLDAAGYQALVG"
     gene            8174..9973
                     /locus_tag="PLL59_08470"
     CDS             8174..9973
                     /locus_tag="PLL59_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY23882.1"
                     /translation="MKKTLLLISLFISILSFAQDGNYDEFLARQFLQNGEYEKAAAYY
                     KDLYEANPSTYYNDYLSLLIQLTDYEKAEKVIKAQYKQAENNPIYLMDLADIYLKQNK
                     EDEAQKQFNKVIKELKADKNNIKQIAQKFIQLKQIELAQKTYLKAKELFKDNNAFNLE
                     IASLLFLKNDIDGMIQAYLNEAALQPDNLSLIETGLQKIFTEEKNKDLLEKELLKRTQ
                     THKRALVYLDLLIWLYMQRNDFDAAVLQAKSLDLLRNEDGGNVLRIARTAAIQQDFDA
                     AIKGYQYVINKGPSFEWYVTANLELINVQRDKIVNSPNYTIAQLLSLKKTYQDFILNF
                     KNEYTTTNAIIELAQLEALYIHEIDTAIQLLLPIVENNYIAKNLLAKAKLNLGDYYII
                     DNQAWESLLLYTQVEKSEKGNPLGEEAKYKNAKLSYYKGDFEWAQTQLKIIKANTSEF
                     ISNDAIDLSVFILDNLNTDDTDFALLLFAKADLLRFQNKLNAAEDTLNKIIAFYPSTA
                     LIDDILYMKYKIEKDRQHYEKAAAYLEEIVAQHSDDLLADNALFYLGDLYQKYLKDEE
                     KAKTYYEKIILDFKDSTFTIEARKRYRKLRGDI"
     gene            10207..10896
                     /locus_tag="PLL59_08475"
     CDS             10207..10896
                     /locus_tag="PLL59_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HMY23883.1"
                     /translation="MGGIYLISILIMGVSWYVSSTLQRRFKQYSQMPIRMSGKEVAEK
                     MLRDNGIFDVKVISVPGALTDHYNPLDKTVNLSDWVYAQSNVAAAAVAAHECGHAVQH
                     NTAYSWLTMRSKLVPVVQVSSKYVQWVILGGLGVLMFTKSPWILLIGIILFAMTTLFS
                     FVTLPVEFDASARALKWLDSTQILVGEEHDKAKNALKWAASTYVVAALGSLVTLLYYI
                     SIFLGARSRED"
     gene            11132..11584
                     /gene="rplM"
                     /locus_tag="PLL59_08480"
     CDS             11132..11584
                     /gene="rplM"
                     /locus_tag="PLL59_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HMY23884.1"
                     /translation="MNTLSYKTKTVTPKDTVRKWYLVDAENQTLGRISSEIAKILQGK
                     NKPSYTPNIDTGDHVVIINAEKVVLTGKKLTDKVYIRHTGYPGGQRFMTPKEAFAKKP
                     EFVLENAISHMLPRTRLGRAMFKKLHVFAGAEHTHTAQKPEVLKFNKI"
     gene            11587..11979
                     /gene="rpsI"
                     /locus_tag="PLL59_08485"
     CDS             11587..11979
                     /gene="rpsI"
                     /locus_tag="PLL59_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HMY23885.1"
                     /translation="MAKETINGLGRRKTAVARVYLKSGKGVITVNGKDIKEYISLKQL
                     VDRALQPLNVLDAVAKFDISVNVKGGGIKGQAEAISLGIARALVKNDETVKPALKAEN
                     LMTRDSRMVERKKFGKKKARKSFQFSKR"
     gene            12004..>12398
                     /gene="rpsB"
                     /locus_tag="PLL59_08490"
     CDS             12004..>12398
                     /gene="rpsB"
                     /locus_tag="PLL59_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMY23886.1"
                     /translation="MAISKLSQQEMLDAGVHFGHLKKKWNPKMLPYIFMERKGIHIID
                     LNKTQEKLEEAAAALRSISKSGRKILFVATKKQAKEIVAEAAKQVNMPYVTERWLGGM
                     LTNFVTIRKSIKKKQNIEKMLGDGALNNIT"
BASE COUNT         4448 a         2171 c         2201 g         3578 t
ORIGIN      
        1 atagaaatac agatttttta ttaacaaagc ctaaaacaac aattgattta agagatccaa
       61 aaataattga caaaaaagta gaatacgacc cacaaacaca aaaatatgtt ctctacgaaa
      121 aaataggcga cacctactac aaaacaccta cctatatgac attcgatgaa tacatcgaat
      181 atactgctcg cgaatctgaa caaggctatt tcttgcagcg ctctcgtgcc attgacttag
      241 ctgagcgcaa aagcaaacaa ccttttttgt atcaaggtcc tgagttattt aatcgatttt
      301 taggtggcac aaaaatagaa attaaaccac aaggaaatgt tgatgtaact attggcgtaa
      361 actcacaacg cgtggagaat cctgtgttgc tacaaaacca acgtcgcaat atcaactttg
      421 attttgacat gaatatccaa ttgggtttac aagcctccat tggcgacaaa atgaaattag
      481 gcattaacta caatactaaa tcaggatttg cttttgaaaa tcaattaaaa ttaggatata
      541 aaggaaaaga agatgacatt atccaagcct tggaatttgg taatgtcagc atgcctttgc
      601 gtagtgcatt aatcaaagga cctcaaaatt tatttggcgt aaaaacacaa cttaaatttg
      661 gcaggctaac gattacaaat gtattggcac agcaaaaatc aaaaaccgaa aatgtacgca
      721 ttgaaaatgg cgcgcaaaca aaaaaattcg ctattaaagc tgacgaatat gaagataatc
      781 gccacttttt tatagcccaa tattttagag atacttacga acaaagtttg agcaaactgc
      841 catttgttgc tgcgcaaact gtcattaatc gtattgaagt ttgggtaacc aacaaaactc
      901 gccaaacaga agaagtgcgc gaagttgtgg catttcagga tttaggtgaa acttctaaaa
      961 tattcaacac tacagcagta acaagtaatg gtggcactta tcctgataat aagtcaaata
     1021 cattatacaa cacaattaca cgaagtagca actcactcga cttccgcgac ccaagtcgat
     1081 gtattagttt tttggaaggc aaaggcttgc aagctgttga tgactttgaa aaagtaagtg
     1141 ccagaaaatt ggctaccaca gagtattatt tccatccaca gttaggctat atttccttaa
     1201 atcaacaatt gcgaccagat gaagttttag gcgttgccat tcaatataca gtcaacggcg
     1261 aagtatttca aattggtgag ttcgcaacag atttaccacc attagcagat acgactaaaa
     1321 caaaagatca aatcttagct ttgaaaatgt taaaggcaac atccgtacgc acggtagagc
     1381 ctatttggga tttgatgatg aaaaacgtgt attcgcttag tgcctacaac gtaagccctg
     1441 aacaattttt ctttgacatt tattatattg atccaggtgg cggccagaaa agatatctgc
     1501 cagatgctgg gcccattact ggcaagcaat tattacaagt tctgaatttg gataatgtca
     1561 acaaccaact agatccacaa aatgatggtc gttttgactt tattcctaat gtcactatca
     1621 cgcctagaaa tggtaaaatt tatttccctg tattagagcc ttttggaagc tcattagcca
     1681 atgccatcgg cgacccaact attgccaata aatatgttta tcgttactta tacgacacga
     1741 caaaatttgc cgcacaacaa ttccctgaat ttaatcgatt tattttaaaa ggttcttatc
     1801 gtggcaccga caacagcaca ttccgtttac ctggtgcatt taatttgcct caaggctcag
     1861 taacagtgag tgctggcggc aatcagttaa aagaaaacat agattacacc gtcaattatg
     1921 gcatcggaga aattgtaatt gttaatcaag gtgtgctcaa ttctggaata cctatcgaca
     1981 ttaagtttga aaataatgta ctctttggtg ttgtcaataa atccttattc ggcacacgac
     2041 tagattacgc agtcaacaaa aacttcacca ttggtttgac gcatatgcgt ttggcagaaa
     2101 agccttttac tcaaaaagta aatttcaacg acgatcctgt taaaaataat atcatcggtt
     2161 tagatatcaa ttatgctact gaatctaaag gcttgacacg tgcttttaat aaaattacgg
     2221 cacaagacat aacgacacct tctaaagttt ccgtagctgc tgaggccgcc tattttaaac
     2281 ctggacacgc aaaagccatc aatattgata atgcaggaac tgtatatgtt gatgacttcg
     2341 aaggcgcgag cactaacttt gatttaaaag gcagtttctt gacttggtca ttagcatctg
     2401 cacctaaagg catgccgaat atttttggcg ttgaaaaatt tccggaagca agaataagtg
     2461 atagtttggt atatggtttt aatcgtgcaa aattagcatg gtattctgtt gatcctgttt
     2521 tccaaagcaa tacaaacagc aatccattaa gcagtgcgct taaacaacaa accttaagta
     2581 gtatttatac acgtcagttt tatgaacaag atatttttat taatcgacaa aatacgacaa
     2641 tcaccaatcc acctttatac actttagatt tatcctattt tcctgaagaa cgtggccctt
     2701 acaattatga aacaacgcct actgtttatt caaaaggtgt aaatgcatca ggaaaattaa
     2761 acaatccaga aacacgctgg gctggaatca tgcgcaccat cgaaggaacg agtgacttcg
     2821 aggctgccaa tatcgagttt gtgcagtttt gggtgttgga tccattcaaa ttcccgggcg
     2881 ctaatagaaa aggaaatcta tacgtacaat taggaaattt atcagaagat attttaaaag
     2941 actccagaaa acaatatgaa aatggacttc ctcgtcctaa tggaggaact acggttgata
     3001 catcaatttg gggcgtaacg cctacaatac aaaatgcatt aaccaatagc ttcgatgccg
     3061 atccagatgt tatcaaaaaa caagatgtag gtttagatgg tttagatgat gatggcgagc
     3121 gttcttatta tgcaaatttt atatcaaatt tgtcttctgt cgttgcgaat cctacaaatt
     3181 tagccaaatt aaataacgac ccttctggcg acaactatgc atttaataga gacaataatg
     3241 tatttacaga aaatgatggc gtaataacac gttataaaga tttttcgaat gcacaaggca
     3301 actcatcgaa taatttaaac aatagcaaca ttaatggcaa tgcaaaaaca cagcctgata
     3361 atgaggattt gaacaacgat aacaccttga acgaaacaga agaatacttt cagtatcgcg
     3421 ttgcctttga tgataatttc ctagccaatt ctccttatat cgtcagcaaa caatctgttc
     3481 catttaatgg caatgattct gcctattggt atcaagtaaa aattccgatt aaagaatttg
     3541 aatctagagt gggtaacatt tctgatttcc gttctattcg ttttgtgcga ttaatcatgt
     3601 ccgattttac agacagcgtc accatgcgtt ttgcacaatt tggtttagtg agaaatcaat
     3661 ggagaaaata tgatttatca ttagcaaatc ctggcgagca attaccaggt gatgaatcgt
     3721 caaatactga atttaatgtt acttctgtaa gtgtggaaga aaatgcaagc agaagtccaa
     3781 ttccttatgc tattcctcca ggtatcatac gcgaacaaaa cataagcggt tataacaatg
     3841 cattgcaaaa cgaacaagcg atgagcttgc aagtttgcga attgcaagat ggcgacgcac
     3901 gtgctgtttt taaaacaaca aatttagact tgcggaatta caaacgcatc aaattatttt
     3961 cacatgctga aaattttcca ggcgacagag gtagctcatt tccaataaaa gacaatgaca
     4021 tcagcttgtt tatgcgtatt ggctctgact ttacagacaa ctattacgaa tatgaattgc
     4081 cactaaaagt tacgcaacct ggcaactata atcctgaaag cgaattagac aaacaactca
     4141 tttggccaga ttctaatcaa ttgaatattt tactggacag ccttactaca gtaaaacaaa
     4201 tcagaaatca aagcaatgcg tcattgctat tgccgtatga tgtcaccttt gcaaatggca
     4261 gtaaaatcag cataaaaggt aatcctgatt taggtgtcgc atctgtgttt atgttgggtt
     4321 taaaaaatcc taaaagaatc atcggcgaga atgagaatac agatgatggt ttagcaaaat
     4381 gtgcagaagt ttgggtgaac gaattgcgta tgtcaggatt tgatgaacgt agcggatggg
     4441 ctgccttagg tcgtgtagat gtgcaattag gtaatctagg taatgtaatc atggctggta
     4501 atattcacac gattggattt ggtgatttag agcaaaggct caaccagcgt tatcgtgata
     4561 attattatca atatgacatc gctgccaatt tgcaattagg aaatttattg cctgagaaag
     4621 ctggcttaaa aattccggtt tacacacaat tctcacaatc tattagcacg ccgcaatatg
     4681 atccatacga gttagatgta gaattaaaag acaaattcaa taccattaat gccgatggaa
     4741 atttaacaaa agacagaaaa aaagaattga tagactcgac aaagcgcgtc gcacaagatg
     4801 ttactactat caaaagtatc aatgtaacca acctgcaaaa agtacgcacc aatccggaaa
     4861 aagaaccacg tgtttacgat gtagagaatt ttaatttcac gtatgcatat acacaaacag
     4921 acagaagaaa tcctattata gaaaccgaag ttatcaaaaa gcatagaggt tctttaggtt
     4981 ggaatttctc accaaaacca ttggtttggc agcctttcaa aaagataaaa aataacaata
     5041 ttcacctcaa gccggtgaaa gaattcaacc ttaatctaaa accaaattct gtagcattcc
     5101 gcactgatat taatcgccaa tatgcctatc aaaaaattag aaatataagt ggcgatgatt
     5161 tagagataca tccaacattc gataaatatt ttacgtggga tcgtttttgg agcatcaaat
     5221 acaatctaac aaaatctata aattttgatt tcacagcaac caacaaagcc agaattgacg
     5281 agccagccgg cgcattagac acaaaagaaa aaagagatac cgttcgtaaa aacttctggc
     5341 gatttggtag aaatatcgac tatagtcatg tatttaatgc atcatacaat ttgccattta
     5401 atttatttcc ttctttagat tggataaatg tgcgcactcg ttatggcgct acttattcat
     5461 ggctggcggc accacttgca ctcactagcc tcggaaatac tatacgcaac agtcaggatt
     5521 accaagtaac agccgatgtg aattttaaaa acttatatca aaaatcaaag tttttaaaac
     5581 catacacttt accggatatc aaaaaaacga agcaagagta tgcagatgct tatgcgaagt
     5641 ataaggattt agacgctgct gcaaaaacaa aaattattaa gaaaaaagag gacattgaga
     5701 aaaagataaa cgacatcgaa ctagcagaaa aagatacgtc taaaacaaaa gaagacatca
     5761 aaaaattagt agatgagaaa aaacaattaa agaatgattt gcgtaaatta aaattagaaa
     5821 aacgtcaatt attagaacct gcaaatcctg ctttagatta tgtaatgcgt ccattgatga
     5881 tgttgcaacg tgcctcattt agctatgatg taaaacaaac tacagtatta cctggcttta
     5941 tgccatcacc agttttattt gggcaaaatt ttaagcaaca tgcacctggc gcaggctttt
     6001 tatttggcgc gcagaaagac accaactggc ttaatgcaat tgctgcgaaa gattggatgt
     6061 cgagagatac tacaatcaac tatcagttta tacagacaaa gcaacaaagc atgaacttga
     6121 aagtttcttt agaaccttat cgtgatttta gaatagatat tacattgcaa aaaacaagag
     6181 gcgaaaccta ttccgagttt ttcaaaacca caacggctaa tggcgctttc aaacatttaa
     6241 cgccacaaac taatggaaat atgtcgatgt catttatgat gctaaaaacc gtatttagca
     6301 aagtagataa aaacaacttc agcaatgctt tttataagtt tcaagaatta cgagaattat
     6361 attcgcaaaa acttggcagc ctaaatccaa actctgatac gatatatatt aatgacagca
     6421 ttaaactgcc taattttaga gaaggctatg gtccatttgc acaagaggtt ttggtgcctt
     6481 ctttgattgc tgcatatact ggaaaaaatc cggataatgt gcgtttaaat cctttgaaaa
     6541 cattgccatt gccaaactgg cgattaagct ataatggttt tgctaaaaca aaatggggaa
     6601 agaaattatt tacttctttc aatatcacac atggatatag ttcgacattt agtgtgggtt
     6661 cgtatttaac gaatttaaac tacattaata caccaggtta ttttaacgaa gacttatatt
     6721 ttgtaccgca ggaaatggac tcattaaatg ggaattatta ctcgtattat ctcattccac
     6781 aagttaccat taccgagcaa ttatcgccat tgttaggtat cgaaattagt tggaaaaata
     6841 gcttaatcac caattttgaa tttaaaaaat ctagaacatt gggactgagt ttactcgact
     6901 ataggttagt tgaaacaaga agcactgaat acgttgctgc attaggttat aaattggcaa
     6961 aattcaaaat cccttttaaa atcagaggta aaagaattac actcaataat gacattaact
     7021 tacgtgccga cttctcctat cgagatgata aaactgtaaa ttatcgctta gatcaaaata
     7081 ttgctgaacc aacgcgtggt caaaaaacaa tatcattgtc aacaacaatt gactacattg
     7141 tcaacaacaa actgaatgtt agaatattct atgactttag aagaacaaca cctgctacat
     7201 tagcatctta tccaacaaaa acacatcgag gtggcatcac tttccgtttt tcattgacgc
     7261 catagtatta gacgaaatca aaaaaaagtt ttaccatagt ttgataaact attcgggcaa
     7321 aattgattat tttcgtaaaa aatataacac atgaatattc cagcagaatt aaaatactcg
     7381 aaagagcacg aatggttgaa agttgatggc gatacggcta ctattggcat taccgcattt
     7441 gcacaaggcg aacttggcga tatcatttat gtagatattg attgcgttgg tgacaatgtt
     7501 gaaaaaaatg ctattttcgg taccgtagaa gcagtaaaaa cagtatctga tttatacatg
     7561 cctgttagcg gtgaagtatt ggagtttaat gaagcattaa aagataaacc agatttagta
     7621 aacagcagtc cttacggcga tggctggatg ataaaaataa gattaagtaa tcctgcggaa
     7681 attgatgagc tattagatgc tgctggctat caagcattag taggctaata gcaataattt
     7741 ttctgaacta atccaagatt tgtttaatca tcaacacaaa ttctacaaga taattcctgc
     7801 cctgctttgg tcaggaatta tttttttgct ctgttttttg cctggcaata aattgccaaa
     7861 agaagactgg ctcgataaaa tttttttcga taaaatagtt cacgcctgct tgtatttcgt
     7921 gctatttttt ctgattataa gaattttcaa ttcaaaaacg attggtacat ttttgacggc
     7981 agcattgctt tgcatcatac agggtgtctt aatagaattt atacaaggct cttctttaat
     8041 tcagcacaga agttttgata tttgggatat tatcgcaaat gtctttggcg tctgttgtgc
     8101 aatttttgtg caaagcaaaa aacataagac tatttaactt cctttttagc actatttttg
     8161 atatgtttag tacgtgaaaa aaacattact ccttatttca ctctttattt ctattttgtc
     8221 atttgcgcaa gatggcaact atgatgaatt tttagcacga cagtttttac aaaatggcga
     8281 atatgaaaaa gctgccgcct attataaaga tttatatgag gcaaatccat ctacctatta
     8341 caacgactat ctctctttat taatacaatt aacagactac gaaaaagcag agaaagtcat
     8401 taaagcacaa tacaaacaag ctgaaaataa tccaatttat ttaatggatt tggctgatat
     8461 ttatcttaaa caaaacaaag aagatgaagc acagaagcag tttaacaaag tgataaaaga
     8521 actaaaagca gataaaaaca acatcaaaca aattgctcaa aaatttattc agttaaaaca
     8581 aattgaacta gcacaaaaaa catacctcaa agcaaaagaa ttgtttaaag acaacaacgc
     8641 atttaaccta gaaattgcca gcttattatt tctaaagaat gatatcgatg gtatgataca
     8701 agcttactta aatgaagccg ccttgcagcc cgataatctt agcctcattg agactggttt
     8761 gcaaaaaata tttacagagg aaaaaaataa agatttactc gaaaaagaat tattaaaacg
     8821 aacacaaacg cataaacgtg cactcgttta tctcgactta ttaatatggc tatacatgca
     8881 acgcaatgat tttgatgcag ccgttttgca agcaaaatca ttagaccttt tgcgcaatga
     8941 agatggtgga aatgtgttgc gcatcgctcg aacagcagct atacaacaag attttgatgc
     9001 ggccataaaa ggctatcaat atgtcattaa taaaggacca tcatttgaat ggtatgtaac
     9061 ggcaaatctt gagttgataa atgtgcagcg cgataaaata gtgaattcgc caaattatac
     9121 aatagcgcaa ttattatcgc taaaaaaaac ataccaagat tttattctga actttaaaaa
     9181 tgaatacacg actacaaatg cgattatcga attagcacaa ttagaagcct tatatattca
     9241 cgaaattgat acagctattc aattgctctt gcctatcgtc gagaacaatt acattgctaa
     9301 aaatttatta gctaaagcaa aactcaattt aggcgattat tatatcatcg acaatcaagc
     9361 ttgggaaagt ttgttattgt acacacaagt agaaaaatca gaaaaaggaa atccattagg
     9421 cgaagaagca aaatataaaa atgcgaaact ctcctattat aaaggagatt ttgagtgggc
     9481 acaaacacaa ttgaaaataa taaaagccaa tacatcagag tttatttcta acgatgccat
     9541 tgatttgtct gtatttattt tagataatct caacacagat gacaccgatt ttgccttgct
     9601 actttttgcc aaggccgact tattgcgttt tcaaaataaa ttaaatgcag cagaagacac
     9661 gctcaataaa attatagcct tttatcctag cacagcgttg attgatgata ttctatacat
     9721 gaaatataaa attgaaaaag atagacagca ttacgaaaaa gcagcagcct atttagaaga
     9781 aatagttgca caacacagcg atgatttatt ggcagataat gccttgtttt atttaggcga
     9841 tttgtatcaa aaatatctga aagacgaaga aaaagcgaaa acctactacg agaaaataat
     9901 cttggatttt aaagacagca ctttcaccat tgaggcacgc aaacgttatc gtaaattgcg
     9961 tggcgatatt tgattaacca cttaagaaac tcaagtagca aaagaatttt taaagaaaat
    10021 aatttaatat ctaaaaggaa actaagtttt cttctgtgta atttctaata ttcttgcatt
    10081 gatatcattt actttttgtt tcttctttcc ttatgtgctt attattattt ttttgtgttt
    10141 aaaattgggt gaaaaatcgg aactatttta gtgctatctt tgttatgtta aaaaatacag
    10201 aaaaaaatgg gtggaattta tttgatttca attttaatta tgggtgtgag ttggtatgtc
    10261 agctccacat tacaacgtag atttaagcag tactcacaaa tgccaattcg tatgtctgga
    10321 aaagaagtag ccgagaaaat gttgcgcgac aatggcattt tcgatgtgaa agtgatatca
    10381 gtaccaggcg ctttgaccga ccactacaat ccattagaca aaaccgtgaa cctgagtgat
    10441 tgggtgtatg cacaatccaa tgtggcagct gccgcagtag cagcacacga atgcggacac
    10501 gcagtgcagc acaacacggc ctactcttgg ttaactatgc gctctaaatt agtaccagtc
    10561 gtgcaagtaa gctctaaata tgtacaatgg gtaattttgg gaggcttagg cgtcttaatg
    10621 tttacaaaat caccatggat attgcttatc ggtatcatct tgtttgcaat gactacattg
    10681 tttagcttcg ttactttgcc tgtggaattt gacgcatcgg cacgagcttt aaaatggtta
    10741 gacagcacac aaatcttggt tggcgaagaa catgacaaag caaaaaatgc tttaaaatgg
    10801 gcagcctcca cgtatgttgt agccgcatta ggttcattgg ttactttatt atactacatt
    10861 tccatcttct tgggtgcacg aagcagagaa gattaaaaaa atattcttac taagaaagtt
    10921 gaaggcaagc tgtatggttt gcctttatct ttttgatctt ttttgacagt acaaactaag
    10981 cgtgataaaa aatctttgtc catacaaaag ctcattttca atgagttgtg ttaaatgaat
    11041 ataaaatatt ttgtctattt cttggtttat caactccaaa aaagtatctt tgcactccaa
    11101 ttttaaagaa aacaagaaaa atttctacaa agtgaataca ttatcttaca aaaccaaaac
    11161 cgttacgcca aaagatactg tgcgtaaatg gtatttagta gatgctgaaa atcaaacact
    11221 tggacgcatc tcttctgaaa tcgcgaagat tttacaaggc aaaaacaaac cttcttacac
    11281 accaaacatt gacacaggtg accacgtagt gatcatcaat gcagagaaag tggtattgac
    11341 aggtaaaaaa ttgacagaca aagtgtatat ccgtcacaca ggctatcctg gtggtcaacg
    11401 ttttatgacg ccaaaagaag catttgctaa aaaacctgag ttcgtattag aaaatgcaat
    11461 tagccacatg ttgccacgca cacgcttagg cagagcaatg ttcaaaaaat tacacgtatt
    11521 tgcaggtgca gaacatacgc atactgcaca aaaaccagaa gtattaaaat ttaataagat
    11581 ataataatgg caaaggaaac aatcaatgga ttaggtcgtc gtaaaacagc ggtagcacgt
    11641 gtttacttaa aaagcggcaa aggtgtgatt actgtaaatg gtaaagacat caaagaatat
    11701 atcagtttaa aacaattagt ggacagagca ttgcaacctt taaatgtttt agatgcagtt
    11761 gcaaaatttg acatcagcgt aaacgtaaaa ggtggcggta tcaaaggaca agcagaagca
    11821 atctctttag gcattgctcg tgcattagtg aaaaatgacg aaactgtgaa gcctgcattg
    11881 aaagcagaaa acttgatgac tcgtgattca cgtatggtag agcgcaagaa atttggtaag
    11941 aaaaaagcac gtaagagctt ccaattcagt aaacgttaat cttcaaaaat ttataataaa
    12001 aacatggcaa tttctaaatt atctcagcaa gaaatgttgg atgccggagt tcacttcggt
    12061 catttaaaga aaaaatggaa tccaaagatg ttaccatata tctttatgga gcgtaaaggc
    12121 attcacatca tagacttgaa taaaacgcaa gaaaaattag aagaagctgc tgctgcactt
    12181 cgttctattt ctaaatctgg tagaaaaata ttgttcgtag ctacaaaaaa acaagcgaaa
    12241 gaaatcgttg cagaagctgc aaaacaagta aacatgcctt atgttactga gcgttggtta
    12301 ggtggtatgt tgactaactt tgttaccatc agaaaatcta tcaagaaaaa acaaaacatc
    12361 gaaaaaatgt taggtgatgg tgctttaaac aacatcac
//
LOCUS       DAOQQV010000076        12280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1805_length_12280_cov_8.477628, whole genome shotgun sequence.
ACCESSION   DAOQQV010000076 DAOQQV010000000
VERSION     DAOQQV010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1805_length_12280_cov_8.477628"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PLL59_08495"
     CDS             <1..1124
                     /locus_tag="PLL59_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HMY23887.1"
                     /translation="HVLDEVLKNNAHLETSSAYDIDLIRSLYTKKKIDQKIRIICNGF
                     KPKKYTDSLLTLIKDGFHNVVPVLDNMDEFEAYQNFDGDLNIGVRIAAEEEPKYQFYT
                     SRLGIRQRDVIPFYVEKVKPNAHIKLRMLHFFIDSGIKDSAYYWSELNKCLNMYVDLK
                     QVCPDLNSINIGGGMPIRYSLGSKYDYAYLIEEIVTKIKNFCDEAEIEHPNIYTEFGN
                     FTVGESGCTIFEVIGQKKQNDSELWYMINGSLMTTIPDIWGMNQRFILLPINNWNQGY
                     QRTIIGGLSCDVSDYYNSEVDINQVYMPKIIGDQKLYIGFFHTGAYQDSLSGYGGTKH
                     CLIPSPKKVIIDKDANGKLTYEVFAEEQNAETMLKVLGY"
     gene            1393..2733
                     /locus_tag="PLL59_08500"
     CDS             1393..2733
                     /locus_tag="PLL59_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMY23888.1"
                     /translation="MAIRKKYLLPAFLFLLLVIAGAWNPGQVLTGNSTGKIDTGDTAW
                     MLVASALVLIMTPALGFFYGGMANRKNIISTVFQSVISLGLMSVIWVTFGFSLCFGDD
                     IGGIIGNPFTFKMFQGVGLKPNETFSATVPFVLFAMFQLKFAIITPALITGSFAQRVK
                     FSGYLLFTVLFFIFIYAPVCHMTWHPDGILFKYGVLDFAGGTVVHMTAGLAALAGAIF
                     LGPRSESERTHEFANVPYVIIGTGLLWFGWFGFNAGSALGVNAKAANAFATTNTAAAS
                     AMVAWILVDAMRGYKISSIGACIGAVVGLVAITPACGFVNVGESIAIGAIAAVVSNFA
                     VHFKNKSTIDDTLDVFPCHGVGGMVGMVLTGVFAADYGTGITDWGLAYGETKTFLHHI
                     IALVGVAAFAFIGSYALYFITNLIDPIRVTKEEEELGLDITQHGESYQTLRIDL"
     gene            2900..3019
                     /locus_tag="PLL59_08505"
     CDS             2900..3019
                     /locus_tag="PLL59_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMY23889.1"
                     /translation="MTSTRKTYSQEYKIKAVEFYLQTGASIQSSDERGVLVQR"
     gene            3155..3493
                     /locus_tag="PLL59_08510"
     CDS             3155..3493
                     /locus_tag="PLL59_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23890.1"
                     /translation="MRYYYSLKISTDNLSRDNLEEIIGVKSNCEHATWELEVEEKEED
                     EYVNFIQYFMDIIEDKLPILDSFGVERESITIWMLYAYNNQCNMEFEAEDLLRLGKNK
                     IKLCISCWEE"
     gene            3580..3699
                     /locus_tag="PLL59_08515"
     CDS             3580..3699
                     /locus_tag="PLL59_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMY23891.1"
                     /translation="MTSTRKTYSQEYKIKAVEFYLQTGASIQSSDERGVLVQR"
     gene            complement(3886..4806)
                     /locus_tag="PLL59_08520"
     CDS             complement(3886..4806)
                     /locus_tag="PLL59_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23892.1"
                     /translation="MKIKKFSCSQCGAPKINPYTLPYIICDFCGAFTDIDFSVGMEAW
                     NKDPKRTNKYNQAKIHFENKLAEQLGKNDKAAYQETQYKYWDVYYDAFPEYLPPSINT
                     DEKYSAYLEVCAISSTDAVFDPKWSAKAGEQALLQKAVTYDVVDGKTVANSDAFFKLA
                     HFYIAYVKASFIDFYNQAKFSIMQEILPEDVHLKMKLSMFVQAWIPYLREEDVRQLLL
                     LTNFNNEYIEVKQDNGNRQACQFCSKEIFVPKGSYKVNCEACHKINNVQLIFHCSSCG
                     MENKTPDNPALPATCINCGTKNKLIKPELG"
     gene            complement(4824..5414)
                     /locus_tag="PLL59_08525"
     CDS             complement(4824..5414)
                     /locus_tag="PLL59_08525"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMY23893.1"
                     /translation="MFTGIVEEIAEVIAIKKEGSNLHLTIKSKLSKSLKIDQSVAHNG
                     VCLTVVKKDKDTHTVTAIKETLDITTLSQLKKGTRINLERAMQAGARLDGHMVQGHVD
                     QTAIVTMIVPENGSWNFHFEFVKKPLFSLVDKGSVCIDGTSLTVVKASKNKFQVSIIP
                     YTFEHTIFSTYAISTAVNIEFDIIGKYVERLLKLHK"
     gene            complement(5417..8101)
                     /locus_tag="PLL59_08530"
     CDS             complement(5417..8101)
                     /locus_tag="PLL59_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY23894.1"
                     /translation="MKFYHKIILSLVLLFTIGPLYATHNRAGEITYKHLSGFTYEFTI
                     TTYTKVSGVSGDADRTRLGIAWGDGTFDSLNRLSEIFLDPDIKQNKYIGIHTYSGPFT
                     YVVGVGDPNRIQDIININNSVSTIFYLEDTIKILDPNIIGYNNSPLLLNPPIEYGNVG
                     QVFIHNPNAYDPDGDSLSYSIASPLQAAGLPVAGYSPPNLINPGPDNQISINQLTGEL
                     TWDAPQVAGIYNIVILIKEYKNGIFVGTVMRDLQIIVDATNNQPPKLNVPLQICKIVG
                     DSIIIPVNANDPNLQDKITLSANGAPFIVPTQAATFTVGAPANPIGGTFRWNTHCDHL
                     NKNDYLVVFNAVDNFNSPPLTDQKTLAIKLLAPAPKNLTTTLNLSTKTVTLKWDSLYI
                     CSNNPKFQGFSIWRKKGCNTPIDTCNPNLANLGYEKIGTTINYSFIDNNIRSGNQYSY
                     RVVADFGDKTNAGLILNRFTSIASIERCVTIPADIPLIYNVDIRNTNETTGQIYVEWS
                     KPFAARLDTIINPGPYVIKLYRAVGINGENYTLVKTVTAATFSAITDTSFLDNGLNTV
                     ANAYHYKLDFYARTNDSLGSTESASSVFLSIASAYQSLQLSWTFSVPWVNTSYVIYRK
                     LPGGSSFDSLTTTTLTAYDDINLINDSLYCYKIKAIGTYAIAGLKTPLINFSQEVCAL
                     PKDTTLPCTPILSINNFCTNASLDTAEYKNYLTWTFQQDGFCKVDDIEKIRIYYAKTS
                     TDTLQQIDSIIGSSFNNYTHSLATRSLAACYAIQAVRKNGNVSNLSAKVCVDNCPLYN
                     LPNAFTPNDDSQNDKYTPIYPYRFVEKIDLKIYNRWGNLVFQTEDPDINWNGTDYKTG
                     KELYTGVYYYVCDVFYQTINGIEKTKKPLSGYIHLFRK"
     gene            8330..8767
                     /locus_tag="PLL59_08535"
     CDS             8330..8767
                     /locus_tag="PLL59_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23895.1"
                     /translation="MAEQLPPSTATPPKTHPVKSFFKKLLYTIMTIFVLLVVGSMLYW
                     NSTAEEGYSAGLLINFSKKGLVFKTYEGTLNSGSVSATGGSNTSNNTWTFSVADEEVA
                     AQLNTIMQSADKNVSLHYKEKRGIFPWKGETNRFVDAVEVVKN"
     gene            complement(8764..9741)
                     /gene="meaB"
                     /locus_tag="PLL59_08540"
     CDS             complement(8764..9741)
                     /gene="meaB"
                     /locus_tag="PLL59_08540"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HMY23896.1"
                     /translation="MLTADYYINGILQQDRIVLSKAITLIESTRKEDEAVANDVLKAC
                     LQINKQSKRIAITGAPGAGKSTFINAFGQHLITQNFTLAVLAIDPSSSASKGSILGDK
                     TRMEDLVSNKQCYIRPSASLSHLGGVAYRTRETILLCEAFGFDYIFIETVGVGQSETA
                     VKNMVDLMLLLLLPNAGDELQGIKRGIMEMADIIIINKSEKENDVIAKIAAAQVKHAV
                     HLLSASEKDWSPAVLNVSSIEKTGIEELHEKINSYFIKMHTSGYLTHNRNAQDYYWFE
                     ETIQQELHYQFSTNSNVQQLKASLQEKIKNKEINAVQAARFLVTNFLEN"
     gene            complement(9833..10516)
                     /locus_tag="PLL59_08545"
     CDS             complement(9833..10516)
                     /locus_tag="PLL59_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HMY23897.1"
                     /translation="MKTFSKDRQASITPRIALNLLKEGNTRFIFNLKANRDLLAQVND
                     TVNGQWPFATILSCIDSRTSAELIFDQGLGDIFSVRIAGNIVNTDIIGSMEFACKLAG
                     SKLIVVLGHSKCGAVKGACDHIEMGNLTELLSKLQPAVYQENTTTENRNSSNSVFVEN
                     VSMLNVKRTVKSIIERSYILEQMVENGEIGIIGAMYNIESGKVEFYDDVQFIKDDENP
                     AFNLASLRH"
     gene            complement(10534..12210)
                     /locus_tag="PLL59_08550"
     CDS             complement(10534..12210)
                     /locus_tag="PLL59_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMY23898.1"
                     /translation="MISKVNIVNNLKSDFSSGLVVFLIALPLCLGIALASGAPLFAGL
                     ISGIVGGIVIGSLSKSALSVSGPAAGLTAIVLSAITEIGVFEIFLCAVIVAGLIQIIL
                     GLLKAGSISYYFPSNVIEGMLAAIGIIIILNQLEHIIGIAGIESEEEEFKIITFINAL
                     KNQLHHNGALIIGGVSILILLLWQQPFMKKLNIIPGALVVVAGGILLNEFFIQQQSSL
                     AVTDKNMLVNIPVITNATDFFNSFTLPNFSGFLRADVWMVGITIAVVASIETLLCIEA
                     IDKLDPHKRNTPTNRELLAQGTGNMLSGFIGGLPITSVIVRSSANLGAGAKSKQSAII
                     HGIFLLLSCITIPFLLNKIPLATLAAVLIMTGYKLCKPAVFKHMWQQGKWQFIPFTIT
                     VIAVVATDLLKGVAIGMMVSIAYLLRQNIKNAYYFHRTSYKNGDVVKIELSEEVSFLN
                     KASILLTLDHLPENSMVIIDAQKSKFIDHDVLQVIREFKDIKAKDKNIKVAVNGFKDV
                     YNIENTEYVSMISADFIDISDNPQRTSGTQRTLLHDLIVSNKKNKTTIID"
BASE COUNT         3867 a         2051 c         2129 g         4233 t
ORIGIN      
        1 cacatgtatt agatgaagtt ttaaaaaaca acgcacactt agaaacatca tctgcttatg
       61 atatcgattt gatacgttct ttatatacaa aaaagaaaat agaccaaaaa attcgtatca
      121 tctgcaacgg ctttaaacca aaaaaatata cagattcttt attgacattg attaaagatg
      181 gttttcataa tgtcgttccc gtattagata atatggacga atttgaagcc taccaaaatt
      241 ttgatggcga cttaaatatt ggtgtgcgta ttgcagcaga ggaagagcca aaataccaat
      301 tctatacatc acgcttaggt attcgccaac gagatgtgat tcctttttat gttgaaaaag
      361 taaaaccaaa cgcacatata aaattgcgca tgttgcattt cttcatcgat tctggtatca
      421 aagattctgc ctactattgg agtgagttga ataagtgttt aaatatgtat gtcgatttaa
      481 agcaagtatg tccagattta aatagcatta atattggcgg tggaatgccg attcgttatt
      541 ctttaggttc taaatatgat tatgcatatt tgattgaaga gattgtaaca aagattaaaa
      601 atttctgtga cgaagcagaa attgagcatc ctaatattta cacagaattt ggcaacttta
      661 cagttggtga aagtggctgt acaatttttg aagtcattgg acaaaaaaaa caaaacgaca
      721 gcgaattatg gtatatgata aatggttcgt tgatgaccac tattcctgat atttggggca
      781 tgaaccagcg ttttatttta ttgcctatca ataattggaa tcaaggctat caacgtacaa
      841 ttattggtgg cttaagctgt gatgttagtg actattacaa ctctgaagta gatatcaacc
      901 aagtgtatat gcctaaaatt ataggagacc aaaaactata tattgggttt ttccatactg
      961 gtgcctatca agattcatta agtggttatg gaggcactaa acattgtctt attccatcac
     1021 ctaaaaaggt aattatcgat aaagacgcaa atggtaaatt gacatatgaa gtttttgccg
     1081 aagagcaaaa cgccgaaacg atgttgaaag tattaggcta ctaagctttt tgttacctaa
     1141 attttactgc tattttattt caaaattctt tttgagaaat taacacatta tttattttat
     1201 aatgcaaaat cgattatctg tcagatatac agatatttag caatattttt tcaatcctta
     1261 aaacattata gaaaaaaaaa tgtgaaatat tttctattaa caaaccatta aagaataaaa
     1321 ttgcatgtaa ttttttaaaa atatttcaat tacttagcag aataaattat tcaccaacta
     1381 aattcacaca ttatggcaat cagaaaaaag tatttattac cagccttcct cttcttatta
     1441 ctggttattg caggcgcttg gaatccagga caagtcttaa ccggcaacag cacaggaaaa
     1501 attgacactg gcgatacggc ttggatgctc gtcgcttccg cacttgtact cattatgaca
     1561 ccagcattag gtttctttta tggtggcatg gccaaccgta aaaatattat ttctaccgtt
     1621 ttccaaagtg ttatctcatt aggtttaatg agcgttattt gggtaacatt tggattcagt
     1681 ctttgctttg gtgatgatat cggtggtatt attggcaatc catttacatt taaaatgttt
     1741 caaggggttg gtttaaaacc aaatgaaaca ttttctgcaa ccgttccatt tgtgttgttt
     1801 gcaatgtttc agttgaaatt tgccattatc acaccagcat taattacagg ttcatttgca
     1861 caacgcgtaa aattttctgg ttatctttta tttacagttt tattttttat tttcatttat
     1921 gctccagttt gccacatgac ttggcatcct gatggaatat tatttaaata tggcgtatta
     1981 gactttgctg gcggaacagt agttcacatg acagcaggtt tagcagcttt agctggcgca
     2041 atatttttag gacctcgctc agaaagtgag cgaacacacg aatttgcaaa tgtgccatac
     2101 gttattatcg gtactggttt actttggttc ggctggtttg gttttaatgc gggctctgct
     2161 ttaggcgtta atgcaaaagc agctaatgca tttgctacaa caaatactgc tgctgcatct
     2221 gccatggtag cttggatttt agtagatgct atgcgtggat ataaaatatc atctatcggc
     2281 gcttgtatcg gtgcagttgt cggtttagta gcgataacgc ctgcttgcgg tttcgtaaat
     2341 gttggtgaaa gtattgcaat cggcgctatc gcagcagttg tcagcaactt tgctgtccac
     2401 ttcaaaaata aatctacaat cgatgatact ttagatgtat ttccttgcca tggcgttggt
     2461 ggtatggtag gaatggtttt gactggcgta tttgctgcag attatggcac aggcatcact
     2521 gattggggat tggcttatgg cgaaacaaaa acttttcttc atcatattat tgcgctagtt
     2581 ggtgttgctg catttgcttt tattggctcg tatgcattat attttattac aaacttaatt
     2641 gatccaattc gcgtaactaa agaagaagaa gaattaggtt tagatattac acaacatggt
     2701 gaatcttatc aaactttgag aatagatttg taaaatttgg atatttgttt attaattgag
     2761 acgataggct tactgattta ggtaagccta tttttttatt tcttttcact actgtccgat
     2821 aaaagtaaaa aaagagagcc tggagttcct acctttgagt ggacagatta gttaagagaa
     2881 taaaggcgaa ctttatgtta tgacatcaac aagaaaaacg tacagccaag aatacaaaat
     2941 taaggctgta gagttttatt tgcaaactgg cgcaagcatt cagtcaagcg atgagcgtgg
     3001 cgtgcttgtg caaagatagc tcattaaagt attatatgaa cagcgcacaa gtacgcaacc
     3061 gcacaaatct aatctgcata cttgcgcgag tttgaaaaat ttataataca ggtataaaag
     3121 catctgaata ttttgcaaaa tagataaaga atttatgaga tattattata gtttaaaaat
     3181 cagtacagat aatctatcta gagataattt agaagagata attggagtaa aatcaaattg
     3241 tgaacatgct acatgggagt tagaagtaga agaaaaagaa gaagatgaat atgttaattt
     3301 tattcaatat tttatggata tcatagaaga taaattacca atattagatt catttggtgt
     3361 tgaaagggaa agcattacaa tatggatgtt atatgcatat aataatcaat gtaatatgga
     3421 gtttgaagca gaggatttat tgagattagg aaaaaataaa ataaaattat gtataagttg
     3481 ttgggaggaa taaaaataaa gaaaacatgc taaacgggta tggagttcct acctttgagt
     3541 ggactgatta gttaagagaa tagaggcgaa ctttatgtta tgacatcaac aagaaaaacg
     3601 tacagccaag aatacaaaat taaggctgta gagttttatt tgcaaactgg cgcaagcatt
     3661 cagtcaagcg atgagcgtgg cgtgcttgtg caaagatagc tcattaaagt attatatgaa
     3721 cagcgcacaa gtacgcaacc gcacaaatct aatctgcata cttgcgcgag tttgaagaat
     3781 cttatcaaac tttgagaata gatttgtaaa atttggatat ttgtttatta attgagacga
     3841 taggcttact gatttaggta agcctatttt tttatttttt tacggctatc ctagctctgg
     3901 tttgataagt ttattttttg ttccacaatt gatacaagtt gctggcaatg ctggattatc
     3961 tggtgtttta ttttccatgc cacaagaaga acaatggaaa attaattgca cattatttat
     4021 tttatgacag gcttcacaat ttactttgta agaaccttta ggtacaaata tttctttgct
     4081 acaaaattga caagcttgac gattgccatt gtcttgtttt acttcaatgt attcattgtt
     4141 gaagttagtg agcaataaaa gctggcgtac atcttcttct cgcagataag gtatccatgc
     4201 ttggacaaac atcgataatt tcatttttaa atgcacatct tcaggcaaaa tttcctgcat
     4261 gatgctgaat ttagcttgat tataaaaatc aataaaagat gcttttacat aagctatata
     4321 gaaatgtgca agcttaaaaa aagcatcgct gttcgcaacc gtttttccat cgacaacatc
     4381 atacgtgaca gccttttgca atagtgcttg ttcgcctgct tttgctgacc atttcggatc
     4441 aaaaactgca tcagtagaag agatggcaca aacttctaaa taggcactgt atttctcatc
     4501 tgtattaata cttggtggca aatattctgg aaatgcatcg taataaacgt cccagtattt
     4561 atattgtgtt tcttgataag cagctttatc attctttcct agttgttctg ctaatttatt
     4621 ctcaaaatgt atttttgctt gattgtattt atttgttctt tttggatctt tattccatgc
     4681 ttccatacca acagagaaat caatatcagt aaatgcacca cagaaatcac aaatgatata
     4741 aggtaaagta taaggattta ttttaggtgc gccacattgt gaacagctaa attttttaat
     4801 tttcatttcg gttcttttat tgcttactta tgtaatttca ataaacgttc tacatatttt
     4861 ccaatgatgt caaattcaat atttactgct gtactaatgg cgtatgtcga aaaaatggtg
     4921 tgttcaaatg tatatggaat aatggacacc tgaaatttat ttttgcttgc ttttactact
     4981 gtcaagctag taccatctat acaaacagaa cctttatcaa ccaatgagaa taaaggtttt
     5041 tttacaaatt caaaatgaaa attccaactt ccattttcag gcacaatcat agtcacaatg
     5101 gctgtttggt caacatgtcc ttgcaccata tgtccgtcaa ggcgagcgcc ggcctgcatg
     5161 gctctttcca aatttattcg agtgcctttt tttaattgtg ataatgttgt aatgtccaat
     5221 gtttccttta ttgcagtgac agtgtgcgta tctttatctt tttttactac tgtcaagcat
     5281 acaccattat gtgctacact ttgatcaatt ttcaaggatt ttgacaattt actttttatg
     5341 gttaaatgca aattgctgcc ctcttttttg atagcaataa cttcagcgat ttcttctacg
     5401 attcctgtaa acatatttat ttcctaaata aatgaatgta accacttaat ggtttttttg
     5461 ttttttcaat gccgtttatt gtttgataaa aaacatcaca cacataataa tagacacctg
     5521 tatataattc ttttccggtc ttataatcag tgccattcca gttgatatca gggtcttctg
     5581 tttggaatac taaattgccc caacggttat atatttttag gtctattttt tctacaaatc
     5641 gatatggata gatcggtgtg tatttgtcat tttgactatc atcattaggc gtaaatgcat
     5701 taggtaaatt atataatgga caattatcaa cgcatacttt agcagataga ttgcttacgt
     5761 ttccattctt tcgcacagct tgtatcgcat agcatgccgc caaactacgc gttgctaaac
     5821 tatgtgtgta gttgttaaat gaacttccga ttatactgtc aatctgctgt aatgtatctg
     5881 tagaagtttt tgcatagtag atacgtattt tttcgatatc atcaacttta caaaatccat
     5941 cttgctggaa tgtccacgtc aaataatttt tatactcagc tgtatccaag cttgcatttg
     6001 tgcaaaaatt attaatgctt aatattggtg tacatggcaa tgttgtatct tttggaagtg
     6061 cacatacttc ttgcgaaaaa ttaataagcg gtgtctttaa tccagcaatt gcataggtgc
     6121 caatggcttt tattttatag caatataaac tatcgtttat cagattgata tcatcataag
     6181 ctgttagagt cgttgttgtt aacgaatcaa atgagcttcc tccaggtagc ttcctataaa
     6241 tcacatacga agtattgacc catggaacac taaatgtcca acttaattgt aaggattggt
     6301 aagccgacgc aatgctaagg aatactgacg atgccgattc tgtacttcct aaactatcat
     6361 tcgttctagc atagaaatca agtttatagt gatatgcatt cgccacagta ttgagaccat
     6421 tgtctaaaaa actagtgtcc gtaatagctg aaaacgtagc agcagtaact gtttttacca
     6481 atgtataatt ttctccatta attccaacag cacgataaag ctttataaca taaggaccag
     6541 gattaataat agtatctagc cttgccgcaa atggtttcga ccattctaca taaatttgtc
     6601 ctgtcgtttc atttgtgttt ctgatatcaa cattgtaaat aagcggaata tctgcaggaa
     6661 tggtgacaca tctttcgata gatgcaatac tcgtaaatct attgagtatc aaaccagcat
     6721 ttgttttgtc gccaaagtca gcaacaactc tataactata ttgatttcct gagcgaatgt
     6781 tattatcgat gaacgaataa tttatagtcg ttccaatttt ctcataacct aagttagcta
     6841 agtttggatt acaagtgtcg attggtgtgt tgcaaccttt ttttcgccaa atactaaaac
     6901 cttgaaactt tggattgttg gagcatatat acaaactatc ccatttcaat gttacagttt
     6961 tagtgcttaa gttaagcgta gtagtcaagt tttttggagc aggtgctaat agttttattg
     7021 ctaatgtttt ttggtcggtc aatggtggac tattaaaatt atctaccgca ttaaacacta
     7081 ccagataatc gtttttattc aaatggtcgc aatgggtatt ccatcgaaat gtaccaccaa
     7141 taggattcgc tggcgcacca actgtaaatg ttgcagcttg tgtaggcaca ataaatggcg
     7201 caccgtttgc gcttagcgta attttatcct gcaaatttgg gtcgtttgca ttgactggta
     7261 ttatgatgct atcacctaca attttacaaa tttgcaatgg cacatttaat tttggtggct
     7321 ggttgtttgt tgcatctacg ataatttgta aatcgcgcat aacagtgcca acaaaaatgc
     7381 catttttata ttctttaatg agtataacaa tattatatat tccagccact tgaggagcgt
     7441 cccaagtgag ctctccagta agctgattta tcgaaatttg attgtcaggt ccaggattaa
     7501 ttaagttcgg tggtgagtag ccagccacag gtaagcctgc tgcttgcaaa ggcgaagcaa
     7561 tgctatacga taaactatcg ccatcagggt cataagcatt cggattgtgt ataaaaactt
     7621 gtccaacatt gccatattct attggcggat ttaataaaag tggtgaattg ttataaccaa
     7681 taatattcgg atctaaaatt ttaatagtat cttctaaata aaagatagtg cttacagaat
     7741 tattgatatt gatgatatct tgaatacgat taggatcgcc aacgccaaca acataagtaa
     7801 acggaccaga gtaggtgtga ataccaatgt atttattttg ttttatgtca gggtctaaaa
     7861 atatttcaga taatctattt aaagaatcaa atgtgccatc gccccaggct atgcctaatc
     7921 gcgttctatc ggcatcacca cttacgccac ttacttttgt ataggtggta atggtaaatt
     7981 cgtaggtaaa acctgaaaga tgtttgtacg ttatttcacc agctcgatta tgtgttgcat
     8041 aaagtggacc tatcgtgaac agcaaaacaa gtgacaatat aattttgtgg taaaacttca
     8101 tacgcttaaa attagtgtat tttactaaca tcgtaaatat aaataggttt atactaaatt
     8161 ttagaatttt tgcatgattt ctttgtattc atctcatttc aatgccatct gatatttaat
     8221 aagcattatc atttataata taaacactca ttattgtaaa taataatctt gaatagtaaa
     8281 atggaactcg ggattaaaaa ttaatgttta atttagtgaa aaaataagca tggcagagca
     8341 attaccacca agcacagcga cacctccaaa aacgcatccc gttaaaagtt tttttaaaaa
     8401 actgctatat acgataatga ctatttttgt cttacttgtt gtcggttcaa tgttatattg
     8461 gaattcaaca gcagaagaag gttacagcgc aggattattg attaactttt ctaaaaaagg
     8521 cttagtgttt aaaacctatg aaggcacttt aaattcaggc agtgtaagtg caactggtgg
     8581 tagcaatact tcaaataata cttggacctt ttccgttgct gatgaagaag tggcagcgca
     8641 attaaatacg attatgcaaa gtgcagataa aaatgttagt ttgcattaca aagaaaaacg
     8701 cggcatcttt ccttggaaag gagaaacgaa tagatttgtt gatgctgttg aagttgtgaa
     8761 aaattagttt tctaaaaaat ttgttaccaa aaatcttgct gcttgtacgg cattgatttc
     8821 tttattttta attttttctt gcaaagatgc ctttagctgt tgaacattgc tattggtaga
     8881 aaattgataa tgcaattctt gttgtatcgt ttcttcaaac caataataat cttgtgcatt
     8941 tctgttgtgt gttaaatagc cagaagtatg cattttaata aaataggaat taattttttc
     9001 gtgtaattct tcgatgcctg ttttttcaat agatgaaaca ttcaacactg ctggcgacca
     9061 atctttttca gaagccgata ataaatgaac agcatgtttt acttgagcgg ctgcaatttt
     9121 tgcaatgaca tcattttctt tctcagattt attgataata ataatatctg ccatttccat
     9181 gatgcctcgt tttatgcctt gcagttcgtc gcctgcgttc ggcagcaata aaagtaacat
     9241 caaatccacc atgtttttta cagccgtttc tgattgccca acgccaactg tttctataaa
     9301 aatataatca aagccaaatg cttcacacaa taaaatggtt tcgcgtgtgc gataggcaac
     9361 accacctaaa tgacttaaac ttgctgatgg tcgaatatag cattgcttgt tgctaactag
     9421 gtcttccatt cttgttttgt cgccgagaat actgcctttg ctggcactgc tcgacgggtc
     9481 gatggccaat actgcaagtg tgaaattttg cgtaatcaaa tgctgaccaa atgcattaat
     9541 gaatgtgctt ttgcctgcgc ctggagcgcc tgtaatagca atgcgtttgc tttgtttgtt
     9601 gatttgcaaa caagccttaa gcacatcatt tgctactgct tcgtcttctt ttcttgtgct
     9661 ttcgattaag gttattgctt tacttaaaac aattctgtct tgttgtaaaa tgccattaat
     9721 ataatagtct gctgttagca tacataaaag tattaaaaat ttatgtagct aactaacaaa
     9781 cggagttctt ttaaacaata aaatgcaata gcttttttct gtgaaattgt atttaatgtc
     9841 gtaatgatgc caaattaaaa gctggatttt catcgtcttt aataaattga acatcatcat
     9901 aaaattcaac ctttccagat tcgatattgt acattgcacc aatgatacca atttcaccat
     9961 tttctaccat ttgttccaat atataactac gctcaatgat tgattttact gtacgtttca
    10021 cattcagcat ggatacgttc tcaacaaata cactattgct tgaatttcta ttttctgtag
    10081 ttgtattttc ctgatacact gctggctgta gctttgatag taattccgtt aaattgccca
    10141 tttctatatg gtcgcaggca ccttttacgg cgccacattt gctatgtcca agcaccacaa
    10201 ttaattttga accagcgagc ttacaggcaa attccatact gcctataatg tctgtattta
    10261 caatgtttcc agctattcta acagagaaaa tatcgcctaa accttggtcg aaaattaatt
    10321 ctgccgatgt gcgactgtct atgcaactta atattgttgc aaatggccat tgaccattta
    10381 ctgtatcgtt cacttgtgct aataaatctc tattagcttt taaattaaat ataaatctcg
    10441 tattgccttc tttcaaaaga tttaatgcaa ttctcggtgt gatggaagcc tgtctgtctt
    10501 tactaaatgt tttcattttt tgctatattt ttattaatct ataattgttg ttttattctt
    10561 tttgtttgat acgattaaat catgtaaaag tgttctctga gtgcctgatg ttctttgcgg
    10621 attgtctgaa atgtcaataa aatcagcact tatcatgctt acgtattctg tattttcgat
    10681 gttatataca tctttgaacc cattgacagc aactttaatg tttttatctt ttgctttaat
    10741 gtctttaaat tctcgaataa cttgtaatac atcatgatcg ataaattttg acttttgtgc
    10801 atcaataatt accatgctat tttctggtaa atgatctaaa gtaagtaata tgctggcttt
    10861 gttgaggaaa gaaacctctt ccgataattc tatttttact acatcaccat ttttatagct
    10921 ggtgcgatga aaatagtagg cattcttaat gttttgacgc aatagatagg caatactcac
    10981 catcattccg atggcaacac cttttagtaa atcagtagca acaactgcaa ttacagttat
    11041 ggtaaacgga ataaattgcc attttccttg ttgccacata tgtttaaata cagctggttt
    11101 acatagttta tatccagtca taattaacac agcagctaat gtagccaatg gaattttatt
    11161 taataaaaat ggaatagtaa tacaagataa tagcaagaaa atgccgtgaa taatcgctga
    11221 ttgttttgat tttgcgccgg cacctaaatt ggcggaagag cgcacaatta ctgaggttat
    11281 aggcaatccg ccaatgaaac cactgagcat atttcctgtt ccttgtgcta ataattctct
    11341 attcgttggt gtattgcgtt tatgaggatc gagtttgtct atggcttcga tgcataataa
    11401 tgtttcgata gatgcaacta cagcaatggt tatgccaacc atccatacat cagcacgcaa
    11461 gaatcctgaa aaattaggca aagtaaagct gttaaagaaa tctgttgcat tcgtgataac
    11521 aggtatattc accaacatat ttttatctgt tactgctaag cttgattgct gttgaatgaa
    11581 aaactcattg agtaaaatgc caccagcaac aacgactaaa gcgccaggaa taatgtttaa
    11641 ttttttcata aatggctgtt gccacagcaa caaaatgagt atagaaacac caccaattat
    11701 taatgcgcca ttatggtgaa gttgattttt caatgcattg ataaatgtaa ttattttaaa
    11761 ctcttcttct tcactttcaa taccagcaat tcctataata tgttctaatt gatttagtat
    11821 aataatgata ccgattgcag ccaacatgcc ttcaattaca ttcgatggga aataatagga
    11881 aatacttcca gcttttaata aacctaaaat gatttgtatt aaaccagcta ctataacggc
    11941 acacaagaat atttcaaaaa cgccaatttc tgtaatagcg cttaatacga tagctgttaa
    12001 gccagcagcc ggaccgctaa cacttaatgc agatttactt aaactgccaa taacaatacc
    12061 accaacgatg cctgatatta aaccagcaaa taagggcgca cccgatgcta aagcaatgcc
    12121 taaacataag ggcaatgcta ttaaaaatac aactaatcca gatgagaaat ctgattttaa
    12181 attgtttact atgtttactt ttgatatcat aacttttttt tgattaatta ttttgattac
    12241 tacttcaata ttgtatttac tgtattaagt gctatgtttt
//
LOCUS       DAOQQV010000077        11601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_1963_length_11601_cov_7.579248, whole genome shotgun sequence.
ACCESSION   DAOQQV010000077 DAOQQV010000000
VERSION     DAOQQV010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1963_length_11601_cov_7.579248"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PLL59_08555"
     CDS             complement(<1..1039)
                     /locus_tag="PLL59_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMY23899.1"
                     /translation="MTTTTQETENFDSKAYLDMELLRFSTAGSVDDGKSTLIGRLLYD
                     SKSIFQDQIEAIEAASAKRGEEYVNLALLTDGLRAEREQGITIDVAYRYFSTPKRKFI
                     IADTPGHIQYTRNMVTGASTANLAIILVDARKGIIEQTYRHSYIASLLQIPHITVCIN
                     KMDLVDWDEKIFNQIVHDYKAFASKLDIKDVHFIPISALAGDNVVNRSQNMNWYNGPT
                     LMYLLETIHIGSDLNHIDARFPVQYVVRPMKNEYHDFRGYAGRIAGGVFRVGDKVKAL
                     PSGFSSKIKSIVTMDAELQEAFAPQSVTITLEDEIDISRGDMIVRENNVPQIEQEFDV
                     MLCWMNEKKMVA"
     gene            complement(1092..1997)
                     /gene="cysD"
                     /locus_tag="PLL59_08560"
     CDS             complement(1092..1997)
                     /gene="cysD"
                     /locus_tag="PLL59_08560"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HMY23900.1"
                     /translation="MNDYHLNHLKELESESIFILREVAAQFENPCILFSGGKDSIVVT
                     HLAIKAFYPASVPFTLLHVDTGHNFQEALDYRDELVAKNGLRLLIASVQEAIDKGIVR
                     EEKGFNASRNALQTVPLLEAIEKNKFDCAIGGARRDEEKARAKERFFSHRDEFGQWDP
                     KNQRPELWNIFNGRKHIGEHFRVFPISNWTEMDVWQYIYQEKIEMPSLYFSHKRNVFE
                     RDGVWYADSPYMLKKENEIVEERVVRFRTVGDITCTGAVFSEAATLEDIIQEVAASRT
                     TERGTRADDKRSEAAMEDRKKAGYF"
     gene            complement(2037..2654)
                     /gene="cysC"
                     /locus_tag="PLL59_08565"
     CDS             complement(2037..2654)
                     /gene="cysC"
                     /locus_tag="PLL59_08565"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HMY23901.1"
                     /translation="MSEKASNIHPTFDKILQRQDKELLLQQHAVCIWMTGLSGSGKST
                     IAQGLEKKLHAHGIMTAILDGDNVRTGINNNLGFSEEDRVENIRRVAEINKLFLQNGI
                     VTINSFVSPTADIRNLAKNIIGEQDFFEVYINASFDECAKRDVKGLYKKALNGEIKNF
                     TGLDAPFEAPENAALEIDTTEQSIEESVELIYNFFHSKIKQHNKQ"
     gene            complement(2758..3990)
                     /gene="lysA"
                     /locus_tag="PLL59_08570"
     CDS             complement(2758..3990)
                     /gene="lysA"
                     /locus_tag="PLL59_08570"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMY23902.1"
                     /translation="MELNNGQYEIGGVPVLSLCKEYGTPLYVYDAAIIERQYNRLMNA
                     FPNKNMKISYACKALTNISILKFIKNLGANLDTVSIQEVQLGLKAGFAPKDIMYTPNC
                     VNIDEINKAVGFGVQINIDNISILEQFGHLYGNTYPVCVRINPHIMAGGNKKISTGHI
                     DSKFGISIYQLRHLERVVASNNIRINGLHMHTGSDILDADVFLRGAEILFEAARSFKD
                     LEFMDFGSGFKVKYSADDNTTNIEELGEKLGIRFEEFCKEYGRDLTIMFEPGKFLVSE
                     SGYLFVRTNVIKQTTSTVFAGVDSGQNHLIRPMMYDAYHHITNVSNPNGIQRIYSVVG
                     YICETDTFGWDRKLNEVHEGDILVLHNAGAYGYSMSSNYNSRYRPAEVLVYKGLAKLI
                     RRRENLEDLLATQLDVEF"
     gene            complement(4119..4781)
                     /locus_tag="PLL59_08575"
     CDS             complement(4119..4781)
                     /locus_tag="PLL59_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HMY23903.1"
                     /translation="MNPLSFLDYHLSFEKVQVAKQIFQDSLAKKLRELGIQFPIANLY
                     IRIFKQEKQLEIWASNKEEFQLIATYPFTNFSGTVGPKNREGDLQIPEGFYQITHFNP
                     KSKFHLSLKISYPNDVDVLRNKSIQQLGGDIYIHGGDYTVGCIPIGDENISVLYWLCV
                     NSFAIHPTIPVHIFPAKMEGKQLDELCFNYPEYITFWRTMQTIYLNFQASKKLDNIEA
                     FA"
     gene            complement(4802..5278)
                     /locus_tag="PLL59_08580"
     CDS             complement(4802..5278)
                     /locus_tag="PLL59_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23904.1"
                     /translation="MQIRGKIFTIIGIVLIFGYFISDSLKDPYDPTQVVLIGNDTLGR
                     DWSAFAEANAKQQFMQSQVAEGSPADAYTAQQFNLLGYGIDSNGNYTVLEDPFKRQKF
                     NQDNYATIQSPDGREIVVGYSRDEETENEDNEENFFRKIKTRHQTEIEEPEMRYKD"
     gene            complement(5309..6820)
                     /locus_tag="PLL59_08585"
     CDS             complement(5309..6820)
                     /locus_tag="PLL59_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMY23905.1"
                     /translation="MANATTLPNENLLADNNNNYIADAVQGHPKQLALLFFTEMWERF
                     SFYGMRALLMLFMVQQLQYADVKANLIYGTYTALVYLMPLFGGIAADKILGYRKAIIL
                     GGVLMAVGHLVLAVPTEWSFFAGMAFLISGNGFFKPNISSMVGKLYAPDDARRDSAFS
                     IFYMGVNLGAAIGGLLCGYIGQKINWHYGFGLAGIFMVVGLITFVIGQKSLGNIGLVP
                     KNENGEAVAKQNQIFVYVISLAIVPLFIFLFNHYTLMGKIMFPICIIATITMVAIAYQ
                     QEKEARDRMLTAIVLVAFSVLFWAFYEQGGGSLNLYTERNVNTFGFPAAAINNFINPF
                     YIILLSFPFAWLWISLANKGKEPSTPMKFSLSFFQLGIGFFLFVIGAKLAFDGRVSFL
                     WYALGYLMLTTGELCISPIGLSMITKLSPPKFTGMMMGFWFLASALGQHLAGLIGTLM
                     AIPSESGETTVSAVDSLNIYSGVFMKIFYVSIGGGLVLLLLVPILKKAMHSSK"
     gene            complement(6860..7828)
                     /locus_tag="PLL59_08590"
     CDS             complement(6860..7828)
                     /locus_tag="PLL59_08590"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HMY23906.1"
                     /translation="MKTLLQHKILNVLIKLLVFGGLLIVLYKQLFGNEKIEIAYAHFL
                     LNFKGNFVVLSTVVLLMILNWTIESIKWKLLIDKLHPISWLDAIEGILFGVTFSLFTP
                     SRVGEFGGRVFALNTERKEAIVSTIIGSLAQIVINVSIGALGLLLYIFIYEKHDTYLI
                     SAYAFIYILLLVTVHFCYYNIDIVSIKFSRFPILKKAARYLHIIDLYSNHDLLKLELF
                     SLLRYLIYCIQFVLLLTFFGIKIPFASAIIIVAAIFFIQTINPINIALIDFGFRGNVA
                     ALFLGGFTDNPFAILATTITLWFINLIIPAVIGGVFAFRFRFFNED"
     gene            7973..8548
                     /gene="ruvC"
                     /locus_tag="PLL59_08595"
     CDS             7973..8548
                     /gene="ruvC"
                     /locus_tag="PLL59_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HMY23907.1"
                     /translation="MTDIKKSKQEHIILGIDPGTQVTGYGLILSDGNHMELIAIGCIQ
                     TEKFDDHYDRLQCIYDRVLEIVLAYKPNILAIEAPFFGKNVQSMLKLGRAQGMAIAAA
                     LSQKIPIQEYSPKKVKQSITGNGNAAKEQVAAMLGHILKTDVLPKQLDATDGLAVAVC
                     HHFSMRGAPKGGKKYSGWEAFLKDNADRIVK"
     gene            8932..10422
                     /gene="cysS"
                     /locus_tag="PLL59_08600"
     CDS             8932..10422
                     /gene="cysS"
                     /locus_tag="PLL59_08600"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HMY23908.1"
                     /translation="MSELHIYNTLTRQKELFKPLHPPFVGLYCCGPTVYSDVHLGNTR
                     TFISFDLVYRYLKFLGYNVRYVRNITDAGHLTNDAGEGKNRMEDQAKLEKLEPMEIVQ
                     KYTVGFHDVCRIFNLLPPTIEPTATGHIVEQIEMTQQLIDNGVAYESNGSVYFDVKAY
                     NAKGNNYGVLSGRNIDELIAGYRDLDGQDEKRNSIDFALWKKASPEHIMQWNSPWGSG
                     FPGWHLECSVMSTKYLGKQFDIHAGGMDLKFPHHECEIAQNVGACGHAPVRYWMHTNM
                     LNFNGQKMSKSLGNSILPMQFITGEHPLLDKAYSAMSVRFLFMQSHYSSELDITIKGL
                     QDAEKGLKKLMNAAKFLKVLQFDAVAVDDKQDKLIRDLCNECKIVMDDDFNAPKLVAV
                     LHELATQINIYYSNNKKVNNISQSTYDLLHTTYVAYLFDVLGLNDEEQMGSNEALEKV
                     MELIIDIRKQARDNKDWPTSDKIRDTLKAANITIKDGKDGTTYEIG"
     gene            10388..11461
                     /locus_tag="PLL59_08605"
     CDS             10388..11461
                     /locus_tag="PLL59_08605"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMY23909.1"
                     /translation="MGKMVQRMRLDSSAKIKFQCLTLSASYMECKPKLIVILMFFIML
                     FVVACKPKTNESNVDSSVASTIQKPTFSSDSAFHFIAQQLSFGSRVPGTAAQQQCAAY
                     FENKFKSYGASVVMQRTNVVVYSGKSVPCINIVASYNTDVKRRLLLCTHWDSRPFADR
                     DDSAVAKPILAADDGASGAAILLEIARQLQQKNPQIGIDILLLDVEDYGQPEYELNQK
                     QGDFYCLGTQYWSKNPHVPNYTAENGILLDMVGAKGATFTLEGVSMQYAPAFMKQVWR
                     NAAQLGYSNYFVNDLTDQIIDDHYYINTLANIPTIDIINRTYTTKTGFASHWHTHQDN
                     INIIDKQTLQAVGETVLATIFDF"
     gene            11543..11601
                     /locus_tag="PLL59_08610"
     tRNA            11543..11601
                     /locus_tag="PLL59_08610"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11569..11571,aa:Met,seq:cat)
BASE COUNT         3718 a         2088 c         2075 g         3720 t
ORIGIN      
        1 gtgctaccat tttcttctca ttcatccaac ataacatgac atcaaattct tgttcgattt
       61 gtggtacgtt attttcgcga acaatcatgt cgccgcgcga aatatcaatt tcatcttcta
      121 atgtaatggt cacgctttgc ggtgcaaatg cttcttgcag ctcagcgtcc atggttacga
      181 tggattttat ttttgatgaa aaaccacttg gcaatgcttt tactttgtct ccaacacgaa
      241 atacgccacc agcaattctt cctgcataac cacgaaaatc atgatattca tttttcatag
      301 gacgcacaac gtattgtact ggaaaacgag catcgatatg atttaaatca gatccaatgt
      361 gtatagtttc taataaatac attaaggtag ggccgttgta ccaattcatg ttttgtgaac
      421 ggttcaccac attatcacct gctaaggcag aaataggaat aaagtgtaca tctttgatat
      481 ctaattttga agcaaatgct ttgtaatcat gtacaatttg gttaaatatt ttttcgtccc
      541 aatctaccaa gtccatcttg ttgatgcaaa cagtaatgtg tggtatttgt agcaaagaag
      601 cgatgtacga atgtcgataa gtttgctcga taattccttt gcgagcatcg actaaaataa
      661 tggctaaatt ggctgtggag gcgcccgtca ccatgtttct ggtatattga atgtgacctg
      721 gtgtgtcggc aataatgaat tttctttttg gcgtagaaaa atatctgtac gcgacatcga
      781 tagtgatacc ttgttcgcgt tcggctcgta agccatcagt tagcaatgct aggtttacat
      841 attcttcgcc acgctttgca gatgcggcct cgattgcttc gatttgatct tgaaatatcg
      901 acttgctgtc atatagtaat cttccgatta aagttgattt tccgtcatcg acactgcctg
      961 cggttgagaa gcgcaatagc tccatatcaa gatatgcttt cgaatcaaaa ttttctgttt
     1021 cttgtgttgt tgttgtcatt gtttttattt gcaaaattgg caatttgtga attacttaga
     1081 acagatatgt gctaaaagta tccagctttc tttctgtctt ccatcgcggc ttcgctacgt
     1141 ttgtcatcgg cgcgcgtgcc acgttcagtt gtgcgagatg ctgcaacttc ttgaatgata
     1201 tcttctaatg ttgctgcttc agaaaaaacc gcaccagtac aagtgatatc accgacagtt
     1261 cggaaacgaa cgacgcgttc ttctactatt tcattttctt ttttcagcat atatggtgag
     1321 tcggcatacc atacaccatc gcgctcaaac acgtttcgtt tgtgtgaaaa gtataagctc
     1381 ggcatttcaa ttttttcttg atagatatat tgccaaacat ccatttccgt ccaattagaa
     1441 attggaaaaa cacgaaaatg ttcgccaata tgttttctgc cattgaatat attccataat
     1501 tcaggtcttt gattttttgg gtcccattgc ccaaattcat cacgatgtga gaagaagcgt
     1561 tctttggcac gtgctttttc ttcatctcta cgagcgccac caatagcaca gtcgaattta
     1621 tttttttcta tggcttctaa taaaggcact gtttgtaagg cattgcgcga tgcattaaag
     1681 cctttttctt ctcgcactat gcctttgtcg atggcctctt gaacagaggc aatcaataag
     1741 cgaagtccat ttttcgctac taattcgtcg cggtagtcta acgcttcttg gaagttatgt
     1801 cctgtgtcaa catgtaatag agtaaaagga acacttgctg gatagaatgc ttttattgct
     1861 aagtgcgtga ctacaataga gtctttacca ccagaaaata agatgcacgg attttcgaat
     1921 tgtgccgcta cttcacgcag aataaaaata gactctgatt ctaattcttt taagtgattt
     1981 aaatgataat cgttcatgaa atagtaatga gtagtgagta atgagtagtg agatgactac
     2041 tgtttattat gttgttttat tttagaatga aaaaaattat aaatcaattc aacgctctct
     2101 tcgatacttt gttctgtggt atcaatttct aaagcagcat tttctggtgc ttcgaaagga
     2161 gcatctaagc ctgtgaaatt tttgatttcg ccatttaaag cttttttata taaacctttc
     2221 acatcgcgct tggcacattc atcaaagctg gcattaatat acacttcaaa aaaatcttgt
     2281 tcgccaatga tgtttttggc taagttgcgt atgtctgccg ttggacttac aaaactattg
     2341 atagtgacaa ttccattttg taaaaatagt ttgttgattt ctgccacacg tcgaatattt
     2401 tcaactctgt cttcttctga aaatccaaga ttattgttga tgcctgtacg cacattatcg
     2461 ccatctaaaa tagcagtcat aatgccgtga gcatgtaatt tcttttccaa tccttgtgca
     2521 atagtagatt ttccagaacc agataagcca gtcatccaaa tacagacagc atgttgctgt
     2581 aagagcaatt ctttatcttg acgctgtaat attttgtcga atgttggatg aatatttgat
     2641 gctttttctg acattgttaa gcaaatttac attatttgta cttattctta tagtatatcg
     2701 actttgtaga gtattaagct tgtatgaaga agcatagtat tttcatcaaa aatgtcatta
     2761 aaattctaca tcaagctgtg tagctaataa atcttcgaga ttttcgcgac gacggatgag
     2821 ttttgctaat cccttataca ctaaaacttc tgctggtcta taccttgaat tgtaatttga
     2881 cgacatcgaa taaccataag cgccagcatt atgtaacact aaaatatcac cttcgtgtac
     2941 ttcgtttagt tttcgatccc agccgaatgt gtctgtctca caaatgtatc caacaacaga
     3001 ataaatacgt tgtatgccat ttggattaga aacatttgtg atatggtgat aagcatcgta
     3061 catcatcggt cggataaggt gattttggcc tgaatctaca ccagcaaaaa cggtagatgt
     3121 ggtttgtttg atgacattgg tgcgcacgaa taaataacca gattcgctaa ctaaaaattt
     3181 cccaggttca aacataattg ttaagtcacg gccgtactct ttgcagaatt cttcaaaacg
     3241 aatgcctaat ttttcgccga gttcttcgat attggttgta ttgtcgtctg cactatattt
     3301 tactttaaaa ccactgccaa aatccataaa ttctaagtct ttgaagcttc tagctgcttc
     3361 gaataaaatt tctgcaccac gcaagaaaac atcagcatct aaaatatcag agcctgtgtg
     3421 catgtgtaag ccatttatac gaatattatt agaagcgaca acgcgttcta gatgtcttaa
     3481 ttgataaatg gaaatcccaa atttagaatc aatgtgacca gtggatattt ttttatttcc
     3541 acctgccata atgtgcggat tgatgcgcac acaaactgga taagtattgc cataaaggtg
     3601 accaaattgc tctaaaatgg aaatattgtc gatattaatt tgcacgccaa aacctactgc
     3661 tttattgatt tcatctatgt tcacacaatt tggtgtgtac ataatgtctt taggcgcaaa
     3721 gcctgctttt aaaccaagtt gaacttcttg tatggaaaca gtatctaagt ttgctcctag
     3781 atttttgata aatttcaaaa tactaatatt ggttagtgct ttgcaagcat agctaatctt
     3841 catattctta tttggaaatg cattcattaa gcgattatat tgacgctcga taattgctgc
     3901 atcatacaca tacaatggtg ttccgtactc tttgcaaaga cttagtactg gaacgccgcc
     3961 aatttcatat tgtccgttat ttaattccat aaagatttgt tatactattt ttttgaaatg
     4021 ctaagatagt cattgattta ggatttgaga tttaggatgc aagatttatt gataggttaa
     4081 taatttttaa gatttgacct tttaaaacca tataaatctt acgcaaatgc ttcgatatta
     4141 tcgagttttt tgcttgcttg gaaatttaga tatatggttt gcatggtgcg ccaaaatgta
     4201 atatattctg gatagttgaa gcagagttcg tccaattgtt tgccttccat ttttgctgga
     4261 aaaatatgta ctggaatcgt tggatgaatg gcaaatgagt tgacacaaag ccaatataaa
     4321 acactgatgt tttcatcgcc gattggaatg caaccaacgg tgtaatcacc gccatgaatg
     4381 tagatgtcac caccaagttg ttgtattgat ttattacgca aaacgtctac atcgtttgga
     4441 taactaattt ttagtgataa atgaaattta ctttttggat taaaatgggt gatttgataa
     4501 aacccttcgg gaatttgcaa atcaccttct ctattttttg ggcctactgt tcctgaaaaa
     4561 ttagtgaatg gataagttgc tataagttgg aattcttctt tattgcttgc ccatatttca
     4621 agctgttttt cctgtttgaa aatgcgtatg tataggtttg ctattggaaa ctgaatgcct
     4681 agttctctta gtttttttgc taatgaatct tggaatattt gtttagcaac ttgaactttt
     4741 tcaaacgata agtgatagtc aagaaatgat aatggattca tcgaaaatag atagaaaacg
     4801 tttaatcttt atagcgcatt tcaggttctt cgatttcggt ttgatgtcgt gttttaattt
     4861 tacgaaagaa attctcttcg ttgtcttcat tttctgtttc ttcgtctctg ctatagccta
     4921 caactatctc acgtccatca ggcgattgaa ttgtcgcgta attatcttga ttaaattttt
     4981 gtcgtttaaa cggatcttct aatacggtat aattgccatt agagtcgatg ccatagccta
     5041 aaagattaaa ttgttgtgca gtataagcat cagctggcga gccttctgct acctgtgatt
     5101 gcataaattg ttgtttggca ttcgcttcgg caaaagcact ccaatctctc cctaatgtat
     5161 cgtttccgat taatacgact tgcgtagggt cgtatgggtc ttttaatgag tcagaaataa
     5221 aatatccgaa aattaaaacg ataccgatga ttgtaaatat tttgcctctg atttgcatat
     5281 tgaaataaca gtctttctgt cgtttagttt attttgaact gtgcatcgct ttctttaata
     5341 tgggtaccaa caataataat accaaaccac caccgatgga cacataaaat attttcataa
     5401 acacaccact gtaaatattt aaagaatcta ccgctgatac tgttgtttcg ccactttctg
     5461 aaggaatggc cattaaagtt cctattaaac ctgcaaggtg ctgtcctaac gcggaagcca
     5521 ggaaccaaaa acccatcatc ataccagtaa attttggtgg tgataatttg gtaatcattg
     5581 ataatccgat aggagagata cataattcac ctgtggtgag catcaaatat ccaagtgcat
     5641 accaaagaaa gcttactctg ccgtcaaatg ccaattttgc accgattaca aataagaaaa
     5701 aaccaatccc taattgaaaa aatgataagc taaatttcat tggcgttgaa ggttcttttc
     5761 ctttattagc gagtgaaatc cataaccatg caaatggaaa actcaatagt ataatataaa
     5821 atggattgat aaaattgtta atcgctgctg ctgggaatcc gaatgtgttt acatttcttt
     5881 ctgtatataa atttaaagaa ccgccgcctt gctcgtaaaa tgcccaaaat aatactgaga
     5941 atgctactaa tactattgct gttaacattc tgtcgcgagc ttctttttct tgttgatatg
     6001 caattgcaac cattgtaatt gttgcgataa tgcaaatagg aaacatgatt tttcccatta
     6061 aagtataatg gttaaacaag aaaataaaca agggtacaat tgccaaagaa atgacatata
     6121 caaaaatctg attttgctta gcaactgctt ctccattttc atttttaggc actaatccga
     6181 tattgcctaa tgatttttgc ccgatgacaa atgtaattaa tccaactacc ataaaaatgc
     6241 cagctaaacc aaagccataa tgccagttta ttttttgacc gatatagcca caaagcaaac
     6301 cacctatagc agcacctaaa ttaacgccca tatagaatat tgaaaaggca ctgtcgcgtc
     6361 tagcgtcatc tggtgcatag agtttaccaa ccattgatga aatatttggt ttaaaaaaac
     6421 cattgccaga aattaaaaat gccatacctg caaagaatga ccattctgtc ggaacagcca
     6481 atactaaatg cccaacagcc attaatactc cacctaaaat tattgctttt cgatagccta
     6541 atattttatc ggctgcgata ccgccaaaca aaggcatcaa atacactaat gcagtatatg
     6601 tcccataaat tagattggct ttgacatcgg catattgtag ttgctgcacc ataaaaagca
     6661 tcaacaacgc ccgcattcca taaaaggaaa aacgctccca catttctgtg aaaaacaata
     6721 atgcgagttg cttcggatga ccttgcactg catcggcaat atagttgttg ttgttgtctg
     6781 ctaataagtt ttcattaggt aatgttgtcg catttgccat aggtgaattt taaggcataa
     6841 agataagaat tataatgagt taatcttcat taaaaaatct aaatctaaag gcaaaaacac
     6901 ctccaatgac agccggaatg ataagattga tgaaccaaag tgttatggtg gttgctaaaa
     6961 tggcgaacgg attatctgta aatccaccaa gaaagagtgc cgccacattg ccacgaaatc
     7021 caaagtctat caatgctata tttattggat taatggtttg tataaaaaaa atagcagcaa
     7081 cgataattat tgcactagca aatgggattt taatgccaaa aaatgtcaat aacaaaacaa
     7141 actgtatgca ataaatgaga tagcgtagca aggaaaataa ttcgagtttc aacaagtcgt
     7201 ggttactgta caagtcgatg atatgtaaat atcgagctgc tttttttaat atcggaaatc
     7261 tcgaaaattt tatactgacg atatcaatat tgtaataaca aaaatgcacg gtcaccaaca
     7321 acaaaatata gataaatgcg tatgcagata taagataagt gtcgtgtttt tcataaataa
     7381 aaatatacaa taaaaggcct agcgcaccaa tagaaacatt tataacaatt tgagctaaac
     7441 tgccaatgat tgtagatacg atagcttcct ttctttcagt atttagcgca aaaacacgac
     7501 caccaaattc acccactcta cttggtgtaa acaaggaaaa ggtaacacca aataagatgc
     7561 cttcaatggc atcaagccag cttattggat gcagcttgtc gatgagtaat ttccatttga
     7621 tactttctat tgtccaattc agtatcatta ataaaaccac agtgctaaga acgacaaaat
     7681 tgcctttaaa atttaaaaga aaatgcgcat aagcgatttc aattttttca ttaccaaata
     7741 actgtttata taatacgatt aataagccac caaataccaa taattttatc agtacattga
     7801 gtattttatg ttgtaaaagt gtcttcattt ctagtgctta gacatatttg aactaaaatt
     7861 ttttaggcta cattatttta gatgagttcc aaagataaaa tctttttgta taggaagttg
     7921 atatccaaat caaacatgct attctttcgg aactatatct tatttttaca aagtgacaga
     7981 cataaagaaa tctaaacaag aacatattat tttgggcatt gatccgggca cgcaagtcac
     8041 tggctatggc cttattttgt cagatggcaa tcatatggaa ttaattgcaa ttggttgcat
     8101 acaaaccgag aaatttgacg atcactacga tagattgcaa tgtatttacg acagagtatt
     8161 agaaattgtc ttggcataca aacccaatat tttagcgatt gaggctcctt tttttggtaa
     8221 aaatgtacag tcaatgttaa agctcggacg tgcacaaggt atggccatcg ctgcggctct
     8281 gtcacaaaaa attccaattc aagaatattc gcctaaaaaa gtaaaacaga gcatcactgg
     8341 aaacggaaat gctgctaagg aacaagttgc tgccatgcta ggacatatac tcaaaacgga
     8401 tgtgcttccc aaacaattag acgctacaga tggcttagcg gtggcggttt gtcatcattt
     8461 ttctatgaga ggcgctccaa aaggaggcaa aaaatacagt ggctgggaag catttttaaa
     8521 agataatgcc gatagaatag tgaaatagaa tacgctgttc attttctgcc taccttacat
     8581 taggtatttt ttgcaataaa cttgaattta acattctatt tagaatgatt ctaagtagat
     8641 tttgtatttt tgaacatgct taaattacca tttttacaaa atcttatcag cacagaacta
     8701 gatttgcaaa aaccatgtaa catagattat tcttacaaga aaaaatgttg caaaagttat
     8761 aaaaacggaa aacgttgtaa atcttgtcca aagaaaaaaa aggactaata gctaagccga
     8821 attgttcctt gcatttttac aatatgttca ttgtaaaaaa atgctcgttt atgagtatgt
     8881 aaaatttcca attttcctta tcatcttatt tctaattcct atctttgttc tatgtcagaa
     8941 cttcatattt ataatacact tacaagacaa aaagagctat tcaaacccct acatccacca
     9001 tttgtaggtt tgtattgctg tggaccaacc gtctattctg atgtgcattt aggcaatacg
     9061 cgtacattta tttctttcga tttagtatat cgctatctca agttcttagg atacaatgtg
     9121 agatacgtca gaaatattac agatgcagga catttaacca atgatgcagg cgaaggtaaa
     9181 aaccgtatgg aagaccaagc aaaattggag aagttagaac cgatggaaat cgtacagaaa
     9241 tatactgttg gttttcatga tgtttgtaga atttttaatc ttttgccacc aacaattgag
     9301 ccgactgcga caggtcatat cgtggaacaa atagaaatga cacagcaatt aattgataat
     9361 ggtgtggctt atgagtccaa tggtagcgta tattttgatg tgaaagcata taatgccaaa
     9421 ggcaataatt atggtgtgct ttccggaaga aatatcgatg aattgattgc tggctatcgc
     9481 gatttagatg gacaagatga aaaacgaaat tcaatagact ttgccttatg gaagaaggct
     9541 tctcctgaac atattatgca gtggaactca ccttggggca gcggttttcc aggttggcac
     9601 ttggagtgtt ctgtgatgag tactaaatat ttaggcaagc aattcgatat acatgcaggc
     9661 ggaatggatt tgaaatttcc gcatcatgaa tgcgaaatcg cacaaaatgt aggcgcttgt
     9721 ggccatgcgc cagtacgcta ttggatgcat acaaatatgc ttaatttcaa tggtcaaaaa
     9781 atgagcaaat cattaggaaa ttcaatactt ccaatgcaat tcatcactgg tgaacatcca
     9841 ttgctcgaca aggcatactc tgcgatgagt gtccgatttt tattcatgca atcacattat
     9901 tcatcagagt tagatattac gataaaagga ttgcaagatg ctgaaaaagg attgaaaaaa
     9961 ctgatgaatg ctgccaaatt tctaaaagta ttgcaatttg atgctgttgc agttgacgat
    10021 aaacaagata aactaatacg cgatttatgc aatgaatgta aaatcgtaat ggacgatgat
    10081 tttaatgcac caaaattagt tgccgttttg catgaattgg ctacgcaaat aaatatttat
    10141 tatagcaaca acaagaaagt aaataacatt tctcaatcaa catacgactt gcttcacacg
    10201 acttatgttg cttatttatt tgatgtattg ggtttaaatg acgaagaaca aatgggcagc
    10261 aatgaagctt tagaaaaagt aatggaactc attattgata ttcgtaagca agcacgagat
    10321 aacaaagatt ggccaacttc tgacaagatt agagacacat taaaagcagc caatattaca
    10381 ataaaagatg ggaaagatgg tacaacgtat gagattggat agtagtgcaa aaataaaatt
    10441 tcaatgcttg actttgtcag catcatacat ggaatgtaag cctaaactga ttgttatact
    10501 tatgtttttt ataatgctat ttgttgtcgc ttgtaaacca aagacaaacg aatcgaatgt
    10561 agattcatca gttgcttcaa caatacaaaa accaacattc tcttcagatt cagcatttca
    10621 ttttatagcg caacaattaa gctttgggtc gcgagtgcca ggcactgcag cgcaacagca
    10681 atgtgctgct tattttgaaa ataaatttaa aagctatggc gcatcagttg ttatgcagcg
    10741 cactaatgtt gttgtatatt caggcaaatc ggtgccttgt ataaacatag ttgcctccta
    10801 caatacggat gttaaacgac gcttgttgct atgtactcat tgggactctc gaccatttgc
    10861 cgaccgtgat gacagcgccg tagcaaaacc aattctagcg gctgatgatg gtgccagtgg
    10921 cgccgcaatt ttattagaaa ttgccagaca actacaacaa aagaaccctc agatcggaat
    10981 tgatatcttg cttttagatg tggaagatta tgggcaacca gaatatgaat taaaccaaaa
    11041 acaaggcgat ttttattgtt taggaacaca atattggagc aaaaatccac atgtacccaa
    11101 ctatactgcc gaaaatggta ttttactcga catggtaggc gctaaaggag ctacttttac
    11161 actagaaggc gtctcgatgc aatatgcgcc agcgtttatg aaacaagttt ggcgcaacgc
    11221 agcacaatta ggctatagta actattttgt caatgatctg actgatcaaa ttattgatga
    11281 tcattattac atcaatactt tagcgaatat tccgaccatc gatatcatca atagaacata
    11341 cacgacaaaa acaggatttg cttcccattg gcacacacac caagacaata taaatattat
    11401 tgacaagcaa actttacaag cagttggcga aactgttttg gcgacgattt ttgattttta
    11461 gtaatcaaaa tttcattttt ataattcaat gtaaaaatgt acatttgcac tccgttctaa
    11521 gaaattagaa cattggtcct gtagctcagc tggttagagc acctgactca taatcagggg
    11581 gtccatggtt cgagcccatg c
//
LOCUS       DAOQQV010000078        11410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2011_length_11410_cov_7.870806, whole genome shotgun sequence.
ACCESSION   DAOQQV010000078 DAOQQV010000000
VERSION     DAOQQV010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2011_length_11410_cov_7.870806"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2399)
                     /locus_tag="PLL59_08615"
     CDS             complement(<1..2399)
                     /locus_tag="PLL59_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMY23910.1"
                     /translation="MSITEIAIKRPLLISVIFLTLIIFGFFGYSLLNYNLLPKFEAPI
                     VSIQTVYRGASSEEVQNNVTKKVEDAVSSIEGVDVISSSSQENVSVVIVQLKQSMSAS
                     AAQSDVERKINQIKSDLPENVDDPIINKFSSSEIPVLRLSTFGKVNETELYDLVKQKI
                     KPILSNVEGVGQVRLIGGSEREIEVKLDNEKLQAYNLSTAQVTQMIAIGNLSYPAGNI
                     ESNDNRFTIRLDAKLHTVDDLRNLIIRENKNGSKIRLQDVASVTDAVGTPSSINRING
                     RVGLGLEISKQTDANAVEVSQHVKAKLDTIVKQYADKGFSYEVAIDQSTYTMEAATAV
                     THDLFLAVLIVALVMLVFLHSFRSSMFVLVALPSAMIPTFILMYVMGFSLNLMTLLAL
                     SLVVGILVDDSIVVLENIFRHMEMGKDRVTAALDGRSEIGFTALAITLVDVVVFVPLA
                     MAGGLIGNILREFALVVVFSTLMSLFVSFTLTPLLASRWGKLEILSKDSLWGRLNIWI
                     EAQITKMIEAYGRILQWVLKHKVTLFFMVIGLFGGIGYLGMNGFIGSSFAGSGDRGEF
                     NLILELEPQATLQQTNKVVQEVEDIILSYPEVTKVFANVGAVTTQMGSFLETPNKADM
                     TVSLVNKLDRTRSTEELGAIIRERLDSIPGIKFSIKPASITGGNQPQIQIVVMGADLD
                     EVWKYANKVHDIIKKTPGTDYVDYSTSKSKTEIEVELDREKIAKMGLNIQTVGYAVQL
                     AFRGNDQSKFKDKGEEYAINIMYDKGDKQSISNIANTTIQTPTGAMIRLGDIATIHEI
                     QG"
     gene            complement(2483..3538)
                     /locus_tag="PLL59_08620"
     CDS             complement(2483..3538)
                     /locus_tag="PLL59_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMY23911.1"
                     /translation="MKNKIIIGIIAVLLIGGMVYKLMANKKIIDKNAILIDRTNVPVS
                     VTTFEASYFPISTDYSLPAVLDINKSGIITATQPGRLASFNIEIGQHVSKGQLIGKID
                     SKQREIGIKSSEATIKKLEDDIIRTKDLIAGDAAPATSINDLNYNLETTKIQKENMLQ
                     QIADNNIYAPISGTIVQKNANAGEFANPGTPLASIMDISILKAIVYVSENNVYDLKVG
                     QTAQITSSIFPDKITRGIIKYIAPKGDENHNYRVEVEIPNSGYKAGTYVQVKFSFKKP
                     ADALQIPKNALVEGIKNPYVYIVNGNTVAVRKLELGEEIGQNVVIRSGLTAGEKVVTS
                     GQINLSEKSKIQIVNNK"
     gene            complement(3565..4890)
                     /locus_tag="PLL59_08625"
     CDS             complement(3565..4890)
                     /locus_tag="PLL59_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMY23912.1"
                     /translation="MKQSIIIVLLSTLFFTSHAQTTYTLSQCIEYALKNHKSIKMSAN
                     DVETAYARKREGQSAYMPQVNAQVKWDDNLILQSSVIPSLSLGSFTTPEQVVKFGNQY
                     NTLAGIQLDQMLFNWSYIQGIKAINPGYDIAKTKKMKTEEDVVYNTIVAYYNIYLINE
                     NEKLLKQNEERINKTMPIVKLQYEKGVIRKIDVDKVQVNYNNIVTQQQILAANKDIAL
                     NNLKYQIGMPLDSSIAIDTNRTNEIVDADNYVDTSNIKNKIEMQLMRKNIYLQEILLE
                     RTKAAYYPQFSFYAKYGGLAFGNKFSESFTRWSQFATIGFKLDIPVFDGLRAASAIKM
                     GKLNIANLKEDMALKEEGLKLQMLNAKASMQNSEQSLILNKENIALAKNVYDVATLQY
                     QKGVISYTEWMSSEYAYKETEQNYLTSLVKYLQAKIEADKSNNNLNIYK"
     gene            complement(4887..5519)
                     /locus_tag="PLL59_08630"
     CDS             complement(4887..5519)
                     /locus_tag="PLL59_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMY23913.1"
                     /translation="MEKLIEYKNVNVSTKIIEKARYLFNQIGVRNTTMDDLARELGIS
                     KKTLYKEIDNKADLVRICVLSDLKAMENKIKDISAMYDNAIEEMLVINEEIIDDIKVY
                     HPSVLHDLMKFYPECCEMIETHKSSFAHQSIANNLAKGIKQGFYREDIRIELVTLFQI
                     NLSMLPLEQKVMLYDTKDMFFEILKFYIHAITTPKGIKEFYELINKRDKK"
     gene            5628..6620
                     /locus_tag="PLL59_08635"
     CDS             5628..6620
                     /locus_tag="PLL59_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HMY23914.1"
                     /translation="MNALVLKTDTFQLENVITPTPQSNQALVKIHYAALNHRDQWIRD
                     GQYAKIQLPAILGSDACGEVIAVAEQNNNHWIGKNVVINPNIDWGNNLHFQQKEYQIL
                     GMPSQGTLAEYIVVNIDRLCEKPNYLLDEQAAALPLAGMTAYNALFNKGNVQKHTNVL
                     ISGVGGGVAQFAFLFAIAVGANVFVTSSKQAVIDECKKLGAQDGANYTEKDAIKNMAK
                     ANGGFDVIIDSACGDGMNELIATLKPTGKFVFYGATRGLPKDLNMRAIFWNHLQLLGS
                     TMGSDTDFKNMIALCNEYQIIPIIDKVFEFDKAVAAFDRMKDGNQFGKIIVKIH"
     gene            6648..7235
                     /locus_tag="PLL59_08640"
     CDS             6648..7235
                     /locus_tag="PLL59_08640"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HMY23915.1"
                     /translation="MDLQQLMNGAIGQQATQLISSQLGIDANQAQSAVGLALPTLLNA
                     LNKNASTNEGAASLYNAITKDHANGGVEDLAGMAQNALSSGEGSSILKHILGGLQPNV
                     ENAISQNAGIGGGQAGQILQILAPLVLKTLGNQTQSQGLDIGGLAGLLTNVVSNQQQQ
                     TAPQQNDIISQLLDRDKDGSVIDDVAGLLGNLFKK"
     gene            7332..8759
                     /locus_tag="PLL59_08645"
     CDS             7332..8759
                     /locus_tag="PLL59_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HMY23916.1"
                     /translation="MHEEILSTLFHQYFKEHCDTIIQLPQAGSDRIYFRLQGKNHAAI
                     GTYGKDTRENETFIHHTKHFHQKFIAVPEVYCISDDKQYYLQQDLGEISLYSIIKQEG
                     ISSNVIAYLKEVLQQLTYLQIRGAEHFNFETCYPIQAFNRSSMFWDLNSFKYYFARVA
                     RVHFSEIKLNEDFHTLSNYLLEETNQYFMFRDCQSRNVMIHDGKPYFIDYQGGRKGPL
                     QYDAASLLWQAGAKIPYAVREELFDFYTKKVAALIYVNKETFKERYHAIVLIRMLQVL
                     GAYGFRGLIEKRSHFIESIQPALENVKWFLENIQLKIELPELTNVLQQLIVSDVFTVH
                     KIDASQTSLKIDINSFSYKRGIPSDASGNGGGFVFDCRGLHNPGRYEPYKALTGKDKP
                     VIEFLESQSKVKVFLENIYKVVDVNIQNYIERDFEHLQINFGCTGGQHRSVYCAEQTA
                     KYIKEKYNITVQIKHIERELQGQFI"
     gene            8761..9522
                     /locus_tag="PLL59_08650"
     CDS             8761..9522
                     /locus_tag="PLL59_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMY23917.1"
                     /translation="MKAMIFAAGFGTRLKPLTDSCPKALVKVGDKPMLQHTIEHLIKH
                     NFDEIIVNIHYLGDQIKAFLQQHNNFGIRIEISDETDLILETGGGLLKAQHFFDDNEA
                     FLVCNADVFTNINVSEMYRFHQSNGSLATLAVRNRESTRYLLFDDNNILYGWENIKTN
                     VAKIPRKIPEAVKANFAPGDTYPLHEFAFSGYHIISPEIFKHQSLLGVFSMTDWYLDL
                     CRNHTIVAYQHNNDVWVDIGSFAQLQKANELIATM"
     gene            complement(9506..10372)
                     /locus_tag="PLL59_08655"
     CDS             complement(9506..10372)
                     /locus_tag="PLL59_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMY23918.1"
                     /translation="MKNKNYFLLFAIIFIANACQKKAEEASPITGTFTAMSYNVAGLP
                     QGINSDQFPELHMPMISPRLNNYNIVAVQEDFYYHKELVQENHHQYKSKYFALEGGLG
                     DGLNQFSDYLMLNYMRTGWDDCHGTDCYTPKGFTYSRVKITPKVYIDVYNLHCNAGSD
                     SADLAARRKNILQLCKYINLRSKDNAVIVLGDFNCRYTRTGDNIRELLSLGFKDAWIE
                     KERNGILPTQNDIPLLGVEVVDKIFYKSNSTIELKALEYVMPTTDFLDNDGNWLSDHR
                     PIYTKFQFTLLQ"
     gene            complement(10429..10863)
                     /locus_tag="PLL59_08660"
     CDS             complement(10429..10863)
                     /locus_tag="PLL59_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23919.1"
                     /translation="MFLLALSSTAYAQYSVFTGFWKGYLITDTSNIDNKKGLPVTVFI
                     SDDNNQGEVAGEMSIQYRYQTDVYKAKYMITGTYDETMTSLLITQEKIVYYDLLPKGL
                     QWCFGSGSFTLERNPYKKRYYLDGIMTTNCGDEQMRIILIKK"
     gene            complement(10894..>11410)
                     /locus_tag="PLL59_08665"
     CDS             complement(10894..>11410)
                     /locus_tag="PLL59_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HMY23920.1"
                     /translation="GEHQKYSGKKLQYFDAELNQNYVPYVIETSIGLDRMFLRIMSES
                     LKEEALENGETRTVLNLPPALAPIKVAVLPLVKKDGLPEKAREIIDILKFVGRTFYEE
                     KDSIGKRYRRMDAIGTPYCITVDHQTLEDNTVTIRERDTMQQDRIAIADIEKIVGAKV
                     DMNNLLRKAIS"
BASE COUNT         3502 a         2017 c         1958 g         3933 t
ORIGIN      
        1 ccttgtattt catgtattgt ggcaatatcg ccaaggcgta tcattgcacc tgttggtgtt
       61 tgaatagtag tattagcaat attgctaatg ctttgtttgt cgcctttatc atacatgata
      121 ttgatagcat attcttcgcc tttgtcttta aatttagatt gatcattgcc tctaaatgcc
      181 aactgaacag catagccaac ggtttggata tttaatccca tttttgctat tttctctctg
      241 tcgagttcga cttcaatttc agtttttgat ttacttgtac tgtaatcgac atagtctgta
      301 ccaggcgttt ttttgatgat gtcatgtact ttatttgcgt atttccaaac ttcatctaag
      361 tcagcgccca tcactactat ttgaatctgt ggttggttgc caccagtaat acttgctggt
      421 ttgatagaaa attttatacc aggaatactg tctaagcgtt cgcgaataat agcacctaat
      481 tcctctgtag agcgtgtcct atctaattta ttgactaaag aaacagtcat gtcggctttg
      541 tttggagttt ctaaaaagct acccatttgt gtcgtaacgg cacctacatt agcaaatact
      601 tttgttactt ctggataact tagaataata tcttcgactt cttgcactac tttgtttgtt
      661 tgttgtagtg ttgcttgagg ttctaattct agaatcaaat taaattcgcc tctgtcgcca
      721 ctacctgcaa atgaactacc aataaaacca ttcataccta aatagcctat gccaccaaac
      781 aagccaatga ccataaagaa taaagtaact ttgtgtttta aaacccattg taatattctt
      841 ccgtatgctt caatcatttt ggtaatttgt gcttcaatcc aaatgtttaa acgaccccaa
      901 agtgaatctt tgctaaggat ttctaatttt ccccaacgtg atgctaataa gggtgttaag
      961 gtaaatgaga cgaataaact cattaaggta gagaatacga ctaccaaagc gaattcacgt
     1021 aagatatttc caatcaaacc tccagccatt gctaaaggta cgaacactac tacgtccact
     1081 aatgtaatgg ctaatgctgt aaagccgatt tcacttctac catctaaagc cgctgttact
     1141 ctatcttttc ccatctccat gtgacggaaa atattttcta ataccacaat actatcgtca
     1201 accaaaatac ctacgactaa tgatagcgcc aacaaggtca ttaggttgag cgaaaaaccc
     1261 atgacataca tgagaataaa tgttggaatc atggctgatg gcaaggcaac taaaacaaac
     1321 atagagcttc tgaagctatg caagaatacg agcattacta atgccacaat taatactgcc
     1381 aaaaataaat catgtgtaac ggctgtcgct gcttccatcg tataagtaga ttggtctata
     1441 gccacttcat agctaaatcc tttgtcggca tattgtttta caatcgtatc taattttgct
     1501 ttgacatgct ggcttacttc taccgcattg gcatcagttt gtttagaaat ttctagacct
     1561 aaaccaactc taccgttgat acgatttatg cttgatggcg tgccaactgc atcagtaacg
     1621 ctggcaacat cttgtaatct tattttactg ccatttttat tttctctgat aatgagattt
     1681 cgtaaatcat ctactgtgtg tagtttagca tctaagcgaa ttgtaaaacg attgtcattg
     1741 ctttcgatat tacctgctgg ataagacaag ttaccgatgg ctatcatctg cgttacttga
     1801 gctgtagaaa gattgtaggc ttgtaatttt tcgttgtcta attttacttc tatctcgcgc
     1861 tctgatccac caattaatcg cacttggcca acgccttcta catttgataa aataggtttt
     1921 attttttgct tgactaaatc atatagctct gtttcattta cctttccgaa agtcgataaa
     1981 cgcaatacag gaatttcact cgagctgaat ttattgataa ttgggtcgtc cacattttct
     2041 ggtaaatcgc tttttatctg attgattttt cgctccacat cagattgtgc agcgctggcg
     2101 ctcatagatt gtttaagctg tacgataacg acagaaacgt tctcttgcga agaggaagaa
     2161 attacatcga cgccttcgat agaagaaacg gcatcttcta cttttttagt tacgttattt
     2221 tgtacttctt ctgacgaagc acctctatat acggtttgta tggatacaat gggtgcttca
     2281 aattttggta atagattata gtttagtaag ctatagccaa agaatccaaa aataatcaag
     2341 gttagaaaaa taacactgat aagtaatgga cgttttattg caatttcggt aatagacatt
     2401 gttgctgttt tattttttaa ttactgatat acgcatgact ttattgaaaa gtaattgaag
     2461 tgttctgcgt gaattcattt aattatttat tatttacgat ttgtatttta cttttctcgc
     2521 ttaaatttat ttggccagaa gtcactactt tttcaccagc agtaagccca cttctgatta
     2581 cgacattttg accaatttct tcaccaagct ctaatttgcg tacagcaaca gtatttccat
     2641 ttacgatata tacataagga tttttaatac cttcaaccaa tgcatttttt ggtatttgaa
     2701 gtgcatctgc tggtttttta aagctgaatt ttacttgcac atacgtgcct gctttataac
     2761 cactgtttgg tatctctact tctactctgt aattgtgatt ttcgtcacct ttaggtgcaa
     2821 tgtattttat gattcctctt gtaatcttat caggaaaaat cgaagaggtg atttgagcgg
     2881 tttggccaac ttttaaatca tacacattgt tttcgctaac atacacgatt gcttttaaaa
     2941 tggaaatatc catgatgctt gctaatggcg ttccaggatt agcaaactca ccagcattgg
     3001 catttttttg tacaatagtt ccactgatag gagcatagat attgttgtca gcaatttgtt
     3061 gcaacatatt ttctttctgg atttttgtgg tttccaaatt gtaattcaaa tcgttgatag
     3121 atgttgccgg cgcagcatcg ccagcaatta aatcttttgt gcgaatgatg tcatcttcta
     3181 attttttgat agtggcttct gatgatttga tgccgatttc tctttgctta gagtcgattt
     3241 tcccaatcaa ttgtcctttg cttacgtgct gcccaatttc gatgttgaaa cttgccaatc
     3301 ttccaggttg tgtagccgtg ataataccac ttttattgat atctaatact gcaggtagcg
     3361 agtagtctgt gctaattgga aaataacttg cctcaaacgt tgtaaccgaa actggcacat
     3421 tagtacggtc gataagtatg gcatttttat caattatttt tttatttgcc attaatttgt
     3481 acaccatgcc accgattaat aacactgcga taattccgat tattatttta tttttcatat
     3541 aagtaagttt attggttgtt atttttattt atagatattt agattgttat ttgatttgtc
     3601 tgcttctatt tttgcttgta aatattttac taaagaagtt aaataattct gttcagtttc
     3661 tttataagca tattcagagc tcatccattc tgtataagaa attacacctt tttgatactg
     3721 taatgtagct acatcatata cattttttgc aagtgcaata ttctctttgt tcagtattag
     3781 actttgttca gaattttgca tacttgcttt tgcattcaac atttgtaatt tcaaaccttc
     3841 ttcttttaaa gccatatctt ctttaagatt agcaatattt agtttgccca tttttattgc
     3901 actcgcggca cgcaaaccat caaacacagg aatgtctaat ttgaatccaa tagtggcaaa
     3961 ttgagaccat cttgtaaacg actcagaaaa tttattacca aaagccaatc caccatattt
     4021 agcataaaaa ctaaactgag gataataggc tgctttagtg cgctccaata aaatttcttg
     4081 caaataaata ttcttacgca tcaattgcat ttctatttta ttcttgatat tggaagtatc
     4141 cacgtaatta tcagcatcta caatttcgtt tgttcgattt gtgtcaatgg caatgctaga
     4201 atctaaaggc ataccgattt ggtatttcaa attgtttaaa gcaatatctt tattggcagc
     4261 cagtatttgt tgctgtgtta caatgttgtt ataatttact tgtactttat ctacatctat
     4321 tttgcgaatt acaccttttt cgtattgcaa ttttacaatc ggcattgttt tgtttatgcg
     4381 ttcttcgttt tgtttcaata atttttcatt ttcatttatt agataaatat tgtagtaagc
     4441 aacaatcgta ttataaacaa catcttcttc tgttttcatc ttttttgttt tggcaatatc
     4501 atatcctgga tttatggctt taataccttg aatataagac caattaaaca acatttgatc
     4561 tagttgtatg cctgctaaag tgttatattg attgccaaat tttactactt gctctggcgt
     4621 tgtaaatgag ccaagtgata aagacggaat tacactagat tgtaaaatta aattatcgtc
     4681 ccattttact tgtgcgttca cttgtggcat ataggcagat tgaccttcgc gttttctggc
     4741 ataagctgtt tctacatcat tagcagacat ttttatgctc ttgtggtttt ttaatgcata
     4801 ttcaatacac tgtgaaagcg tgtatgtcgt ttgtgcgtgg ctagtaaaga atagcgtgct
     4861 tagtaaaact ataataatcg attgtttcat tttttgtctc ttttgtttat taactcgtaa
     4921 aattctttaa ttccttttgg tgttgttatt gcatgaatgt aaaatttcaa tatttcaaaa
     4981 aacatatctt tagtgtcata cagcattacc ttttgttcaa gtggcaacat gctaagatta
     5041 atttgaaata aagtaaccaa ttcaatacgt atgtcttctc tgtaaaatcc ttgtttgatt
     5101 ccttttgcta aattgttggc aatgctttgg tgggcaaaag aagatttatg tgtttcaatc
     5161 atctcacaac attctggata aaatttcatc aaatcatgta acacagacgg atgatatact
     5221 ttgatgtcat caatgatttc ttcatttatc accaacattt cttcgatagc attatcatac
     5281 attgcggaaa tgtctttaat cttattttcc attgccttta aatcagacaa cacacatatt
     5341 cttactaaat ctgctttgtt atctatttct ttgtataatg ttttttttga aatacctaac
     5401 tcacgcgcta ggtcgtccat tgtggtattt cgaacaccaa tctggttgaa caaataccgc
     5461 gctttctcta ttatctttgt agagacattg acattcttat attcaatcaa tttttccata
     5521 ttcatctgca aatctatgta gtataaactt aggaaactat aaaaggtttt aaagtttccg
     5581 agaatttaat gttaagaaat tgtagctttg aattttaatt ataaattatg aatgcactcg
     5641 ttctcaaaac agacaccttc caactagaaa atgtgataac accaacgcca caaagcaatc
     5701 aggcattggt taaaatacac tatgctgcgc taaatcatcg cgaccaatgg attcgcgatg
     5761 ggcaatatgc caaaatacaa ctaccagcca ttttaggctc tgatgcttgt ggcgaagtca
     5821 ttgcagttgc tgaacaaaat aacaatcatt ggattggaaa aaatgtcgtt atcaatccaa
     5881 atattgattg gggaaataat ttacatttcc aacaaaaaga atatcagatt ttaggaatgc
     5941 catcacaggg caccttagca gaatatatag tagtcaacat agatagacta tgtgaaaaac
     6001 caaactatct gttagatgaa caggctgctg ccttgccatt ggctggtatg actgcttata
     6061 atgctttatt caataaagga aatgttcaaa agcatacgaa tgttttaatt tctggcgttg
     6121 gcggtggcgt ggctcaattt gcatttttat ttgccattgc ggtaggtgct aatgtattcg
     6181 ttacttcaag caaacaagca gtgattgatg aatgtaaaaa gctgggtgct caagatggtg
     6241 ccaactacac agagaaagat gcgataaaaa acatggcaaa agcaaatggc ggttttgatg
     6301 tcattattga tagtgcttgt ggtgacggta tgaatgaact tattgcaaca ctaaaaccaa
     6361 caggaaagtt tgtgttttac ggtgctactc gtggtttgcc taaagaccta aatatgcgtg
     6421 ccattttttg gaatcatctg caattgttag gctcaacaat gggcagcgat acagacttta
     6481 aaaatatgat tgccttatgc aacgaatacc agattatacc tatcattgat aaagtgtttg
     6541 aatttgataa ggccgttgca gcatttgaca gaatgaaaga tggcaatcaa tttggcaaga
     6601 ttattgtaaa aatacattag caatttttaa caaaataaat aacacaaatg gacttacaac
     6661 aattaatgaa tggcgcgata ggacaacaag ccacacaact aatcagcagt caattaggca
     6721 ttgatgccaa tcaggcacaa agtgcagttg gcttggcatt gcctacttta ttaaatgcgc
     6781 ttaataaaaa cgcatctacc aatgaaggtg ctgcttcctt gtataatgcc attacaaaag
     6841 atcacgctaa tggtggtgtt gaagacttag caggaatggc acaaaacgca ctgagtagtg
     6901 gtgaaggtag ttctatatta aaacacattt taggtggttt gcaacccaat gtagaaaatg
     6961 ccatttcaca aaatgctgga attggtggcg gtcaagctgg acaaattcta caaatattgg
     7021 cgccattagt actgaaaaca ttaggcaatc aaacacaatc acaaggctta gatattggcg
     7081 gcttggcagg cttattaacg aatgtcgtta gcaatcaaca acaacaaaca gcaccacagc
     7141 aaaacgacat tatttctcaa ttattagaca gagataaaga cggtagcgtg attgatgatg
     7201 ttgcaggatt attaggtaat ttatttaaaa aataattttt attttcattc ataaaaaatc
     7261 actcacatcg agtgattttt tttattgtaa tagcgaactt attacttgtt acttatcact
     7321 atatttgcag tatgcacgaa gaaatactga gcactttatt ccatcaatat tttaaggaac
     7381 attgcgatac aattatacag ttgccacaag caggttctga tagaatttat tttcgtttgc
     7441 aaggaaaaaa tcatgcagca ataggtacgt atggaaaaga tacgcgagaa aatgaaacct
     7501 ttattcatca caccaaacat tttcatcaaa aatttattgc tgttccagaa gtatattgca
     7561 tcagcgatga taagcaatac tacttacagc aagacttagg agaaatatct ttatattcaa
     7621 ttattaagca agagggaatt tcaagcaatg taattgcata tttgaaagaa gtattgcagc
     7681 aacttactta tttgcaaatc agaggtgctg agcattttaa ctttgaaaca tgctatccga
     7741 ttcaagcatt taatagaagt tcaatgtttt gggatttgaa ttcattcaaa tactattttg
     7801 ctcgcgtcgc acgtgtgcac tttagcgaaa taaaactcaa tgaagatttt catacgctaa
     7861 gcaattattt gctggaagaa accaatcaat attttatgtt tcgcgattgc caatcgcgca
     7921 atgtaatgat acatgatggc aaaccttatt ttatcgatta tcaaggtggc agaaaaggac
     7981 ctttgcaata tgatgctgct tccttattat ggcaagctgg cgcaaaaatt ccttacgcag
     8041 ttcgagagga actattcgat ttttatacta aaaaagtagc ggcattaatt tatgtcaata
     8101 aagaaacttt taaagaacga tatcacgcca tcgttttgat tcgcatgttg caagttttag
     8161 gtgcctatgg tttccgtggt ttaattgaaa aacgaagtca ctttatagaa agcatacagc
     8221 cagcattaga aaatgtaaaa tggtttttag aaaacataca gttaaaaatt gaattacctg
     8281 agctaacaaa tgtattacaa cagttgatag tttcagatgt ttttaccgta cacaaaatag
     8341 atgcatcaca aacatctttg aaaatcgata tcaatagttt ttcctataaa agaggcattc
     8401 cctcagacgc atcaggcaat ggcggtggct ttgtttttga ttgcagaggt ttgcacaatc
     8461 caggtaggta cgagccttac aaagcattga caggaaaaga taagccagtg attgaattct
     8521 tggaatcaca aagtaaagtc aaagtatttt tagaaaatat ttataaagta gtcgatgtca
     8581 atattcaaaa ttatattgaa cgcgatttcg agcatttgca aatcaatttt ggttgcacag
     8641 gcggacagca tcgcagtgtg tattgtgctg agcaaacagc aaaatacatc aaagaaaaat
     8701 acaatatcac tgtacaaata aaacatatcg aaagagaatt acaaggtcaa tttatttagt
     8761 atgaaagcaa tgatattcgc agcaggtttc ggaacaaggc ttaagccttt gacagacagc
     8821 tgcccgaaag ccttagtaaa agtaggcgat aagccaatgt tacaacatac gattgagcat
     8881 cttatcaaac ataattttga tgaaattatt gtcaatattc attacttagg tgatcagata
     8941 aaagcattct tacaacaaca taacaacttt ggtattcgaa tagaaatttc tgatgaaaca
     9001 gacctgattt tagaaacagg tggaggcttg ttgaaagcac aacatttttt tgatgacaat
     9061 gaagcttttt tagtgtgtaa tgctgatgtg tttaccaata taaatgtatc ggaaatgtat
     9121 cgctttcatc aatcaaatgg ctctttagca acattagctg ttcgcaatcg cgaatccaca
     9181 cgttatctat tatttgacga taataatatt ttatacggct gggaaaatat taaaaccaat
     9241 gtagcaaaga ttccacgaaa aatacccgaa gctgttaaag caaattttgc accaggtgat
     9301 acctatcctt tacacgaatt tgcttttagc ggttatcata ttatttcacc agaaattttt
     9361 aagcaccaaa gcttacttgg tgttttttca atgacagatt ggtatttgga tttgtgccgt
     9421 aatcatacga ttgtcgcata tcaacacaat aatgatgttt gggttgatat aggttctttt
     9481 gcacaactgc aaaaagcgaa cgaacttatt gcaacaatgt aaactggaat ttggtgtaga
     9541 taggtctgtg gtcagacagc caattgccat cattgtctaa gaaatctgta gttggcatta
     9601 catattctaa tgctttcaat tcaatggtgc tattgctttt gtaaaaaatc ttatcaacga
     9661 cttctacgcc taacaaagga atatcatttt gtgttggtaa aatgccattt cgttctttct
     9721 ctatccaagc atctttaaag cctaatgaca aaagctcgcg aatgttatcg ccagtgcgtg
     9781 tgtatcggca attaaaatcg ccgagcacaa ttacagcatt atctttagaa cgtaagttga
     9841 tatacttaca taactgcaat atattttttc tgcgagcggc aaggtcagca ctgtctgagc
     9901 cagcattgca atgcaaatta tatacatcga tatatacttt tggagtaatt ttcacacggc
     9961 tatatgtaaa tccttttggt gtataacaat ctgtgccatg gcagtcgtcc cagcctgtgc
    10021 gcatataatt caacatcaga taatcagaaa attggtttaa gccatcgccc aatcctcctt
    10081 ctaatgcaaa gtatttgctt ttatattgat gatgattttc ctgtactaat tctttgtgat
    10141 aataaaaatc ttcttgcacc gcaacaatat tgtaattgtt gagtcttgga ctaatcatcg
    10201 gcatatgtag ctccggaaat tgatcggaat taataccttg tggcaggcca gcaacattat
    10261 aactcattgc tgtaaatgtg ccagtgatgg gtgatgcttc ttcggccttt ttttgacatg
    10321 cattggcaat aaaaattatt gcgaacaata gaaagtaatt tttatttttc attctttcaa
    10381 gatacgattt atcttaaaat tttaaaatag aaaatatcaa agagctactt attttttgat
    10441 tagaatgatg cgcatttgct catcgccgca gttggttgtc ataatgccat ctaagtaata
    10501 ccgtttttta tatggatttc tttccaatgt gaacgagccg ctaccaaaac accattgtaa
    10561 tcctttaggc aataaatcgt aatacacaat tttttcttgt gttatcaata agctcgtcat
    10621 tgtttcgtcg taggtgccag taatcatgta ttttgcttta tatacatcag tttggtagcg
    10681 atattgtatg ctcatttcgc cggcaacttc accttggttg ttgtcatcag aaataaatac
    10741 agtaacaggc aatccctttt tgttgtcgat gttgctggtg tcagtaatta aataaccttt
    10801 ccaaaagcca gtaaacacgg aatattgcgc ataagctgta ctgcttagtg caagtaggaa
    10861 caatatgata atttgttttt tgatgcttgt aaattatgaa attgctttac gtaataaatt
    10921 attcatatca acttttgcgc caacaatttt ttcgatatct gcaatagcaa ttctatcttg
    10981 ctgcatagtg tcgcgctcac gaattgttac tgtattgtct tcaagtgttt ggtgatcaac
    11041 tgtaatacaa taaggcgtgc cgatggcgtc cattctgcga tagcgtttgc cgatggaatc
    11101 tttttcttcg taaaacgttc tgcctacaaa ttttaagatg tcgataattt cacgtgcttt
    11161 ttcaggcaag ccatcttttt tgaccaaagg caaaacagct acttttatgg gtgctaatgc
    11221 tggtggtaag ttcaatactg ttctggtttc gccattttcc aaagcttctt ctttcaggct
    11281 ctcgctcata atgcgcagga acattctgtc taaaccaata gaagtttcga tgacataagg
    11341 cacataattc tgattcaatt cagcatcgaa atattgcaat ttttttcctg aatatttttg
    11401 atgttcacct
//
LOCUS       DAOQQV010000079        11393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2019_length_11393_cov_7.413565, whole genome shotgun sequence.
ACCESSION   DAOQQV010000079 DAOQQV010000000
VERSION     DAOQQV010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2019_length_11393_cov_7.413565"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..927)
                     /gene="rpsC"
                     /locus_tag="PLL59_08670"
     CDS             complement(127..927)
                     /gene="rpsC"
                     /locus_tag="PLL59_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HMY23921.1"
                     /translation="MGTKANPIANRLGIIRGWDSNWYGGKDFSDKLIEDQKIRKYLNA
                     RIQKGGISKIIIERTLNRITISINTSRPGIIIGKGGNEVDKIKEEIKKITKKDVQINI
                     VEIRRPEIDAAIVGETVCKQLEARVNFRRAVKMAIASAMRLGVEGIKIKCSGRLGGAE
                     MARSEEYKEGRTPLHTFRADIDYAWKEAETIYGKIGVKVWICKGEVFTKRDLSPNIGM
                     EEANKKGGERRDDRRGGERRDDRRGGGDKRGGGKPRGERSENRGPRKK"
     gene            complement(942..1370)
                     /gene="rplV"
                     /locus_tag="PLL59_08675"
     CDS             complement(942..1370)
                     /gene="rplV"
                     /locus_tag="PLL59_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HMY23922.1"
                     /translation="MGQRKKIAADARKEARKSVCIARATNVPTSPRKMRLLADVIREK
                     DVMEALNILTFSTKHASKTMEKLVRSAIANWEQKFETKAEDSELYIKSVMIDSARILK
                     RFQPAPQGRAYRIRKRSNHITVQLASRVAQAEQTTENNEK"
     gene            complement(1381..1647)
                     /gene="rpsS"
                     /locus_tag="PLL59_08680"
     CDS             complement(1381..1647)
                     /gene="rpsS"
                     /locus_tag="PLL59_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HMY23923.1"
                     /translation="MARSLKKGPYVAYHLQGKVDAMNASNKKTVIKTWSRASMITPEF
                     VGHTFAVHNGNKFIPVYVTENMVGHKLGEFSPTRQFKGHSGNRK"
     gene            complement(1658..2482)
                     /gene="rplB"
                     /locus_tag="PLL59_08685"
     CDS             complement(1658..2482)
                     /gene="rplB"
                     /locus_tag="PLL59_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HMY23924.1"
                     /translation="MSTKKYNPITPSLRYRISNSYSEVTTNVPEKSLVVSNKKSGGRN
                     QKGEMTMRYIGGGHKRQYRIIDFKRNKFDIPATVKTIEYDPNRSAFIALVVYKDGEKR
                     YIIAPEGILVGQEIISGKSVAPEVGNTLPLSNMPLGSVIHNIELQPGRGASICRSAGT
                     FAQLSGKDGKYVNIKLPSGEARQILGTCLATIGSVSNSDHSLEVKGKAGANRWAGRRP
                     RTRAVVMNPVDHPMGGGEGRASGGHPRSRKGIPSKGLKTRSKTKASNKHIISRAKK"
     gene            complement(2502..2792)
                     /gene="rplW"
                     /locus_tag="PLL59_08690"
     CDS             complement(2502..2792)
                     /gene="rplW"
                     /locus_tag="PLL59_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HMY23925.1"
                     /translation="MEILKKPLITEKASALSEKAGKYTFLVEKKANKVEIKKAIEKMY
                     GVNVEEVNTAIIASKPKNRNTKTQVISGRKPSYKKAIVKVAKGEVIDFYNEI"
     gene            complement(2804..3424)
                     /gene="rplD"
                     /locus_tag="PLL59_08695"
     CDS             complement(2804..3424)
                     /gene="rplD"
                     /locus_tag="PLL59_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HMY23926.1"
                     /translation="MKAEVYNINGQATGRSIELPAAIFGVEPNEHVIYLAVKQYLAGR
                     RSGTHKTKEKSELSGSTRKLHKQKGTGGSRKGSIKNPLFKGGARIFGPRPRFYDFKLN
                     KKVKSLAKNSALSVKAADSAIKIVEDFTFNAPKTKEYLKFLQAFGLENKKSLLILPEL
                     NDNVYLSSRNVQGANIATADSLNTYEIMNNSTILISEKSIEVLANS"
     gene            complement(3428..4078)
                     /gene="rplC"
                     /locus_tag="PLL59_08700"
     CDS             complement(3428..4078)
                     /gene="rplC"
                     /locus_tag="PLL59_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HMY23927.1"
                     /translation="MAGLIGTKVGMTSIFGEGGAIIPCTVIQAGPCVVTQKKSKDKDG
                     YDAIQLGYGDKKEKNTTKPMKNHFEKSGGAPKAKLVEFKGMDDLNVGDIVTSEILAEG
                     EKVNIIGTSKGKGFQGVVKRHGFSGVGGQTHGQHNRLRAPGSLGACSTPARVMKGMKM
                     GGRMGTDRVKELNMKIVKVLNDENLIIVKGSIPGHNGAYVIIEKSKKSVSLTKGKS"
     gene            complement(4085..4390)
                     /gene="rpsJ"
                     /locus_tag="PLL59_08705"
     CDS             complement(4085..4390)
                     /gene="rpsJ"
                     /locus_tag="PLL59_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HMY23928.1"
                     /translation="MAQKIRIKLKSYDHNLVDKSAEKIVKTVRSAGAVVTGPIPLPTN
                     KKIFTVLKSPHVNKVAREQFKLSTYKRLLDIYSATPKTVDALMKLELPSGVDVEIKV"
     gene            complement(4406..6511)
                     /gene="fusA"
                     /locus_tag="PLL59_08710"
     CDS             complement(4406..6511)
                     /gene="fusA"
                     /locus_tag="PLL59_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HMY23929.1"
                     /translation="MARDLKFTRNIGIAAHIDAGKTTTSERILFYAGVNHKIGEVHDG
                     ASTMDWMVQEAERGITITSAATTVKWNYRGQEYHVNLIDTPGHVDFTVEVNRSLRVLD
                     GLVFLFSAVDGVEPQSETNWRLANNYNVARLGFVNKMDRSGADFLNVVEQVKKMLGSN
                     AVPLQLPIGAEDTFKGVVDLINFRGIIWNEHDRGMTYEVVDIPAEMVEEATMWRERLL
                     EAVSEYDDKLMEKFFDDPSTISEREILDALRSACLDMKIVPMLCGSSFKDKGVQTMLD
                     LVMELMPSPLDRPNIKGTHPDTGEEVVRKPSYDEPFAGLAFKIATDPYVGRLAFTRAY
                     SGVLDAGSYVINTRSGNKERISRIFQMHANKQNPIEKLGAGDIGAVVGFKDIRTGDTL
                     CAEGNPIVLESMVFPDPVIGLAIEPKTQKDADKLGMALAKLAEEDPTFKVRYDEDTAQ
                     TVISGMGELHLEIIVDRLLREFKVECNQGAPQVNYKETITNSIEHREKYAKQSGGRGK
                     FADIQIEIGPGAEDKEGLEFINDIFGGAIPKEFIPSVEKGFKEAMKTGVLAGYEVPSL
                     KVRLFDGSYHQVDSDSFSFEQAAKFAFREACRKANPILLEPIMKLEVITPEENTGDVV
                     GDLNRRRGMMEGMEMKNNANVIKAKVPLSEMFGYVTDLRTITSGRATSIMEFSHYAPT
                     PNNITEQVVAKAKSKVKAD"
     gene            complement(6587..7054)
                     /gene="rpsG"
                     /locus_tag="PLL59_08715"
     CDS             complement(6587..7054)
                     /gene="rpsG"
                     /locus_tag="PLL59_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HMY23930.1"
                     /translation="MRKQKAKKRYLAPDPVYGDPLVTRFVNNLMIGGKKSTAYTAFYE
                     AMERIKASTKEEGYEIWKKAMDNAMPAVEVKSRRIGGATFQIPTPIREDRKLFMTIRW
                     LIQFSRKRNGKSMGDKLAAELIAASKGEGAAVKRKEDTHKMAEANKAFSHFKA"
     gene            complement(7099..7509)
                     /gene="rpsL"
                     /locus_tag="PLL59_08720"
     CDS             complement(7099..7509)
                     /gene="rpsL"
                     /locus_tag="PLL59_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HMY23931.1"
                     /translation="MPTIQQLVRKGREQLQYASKSRALDACPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNKIEVIAYIPGEKHNLQEHSIVMIRGGRVKDLPGVRYHIVRGAL
                     DTAGVSDRQKSRSKYGTKKGKAGAAAPAKGKGKK"
     gene            complement(7911..8174)
                     /locus_tag="PLL59_08725"
     CDS             complement(7911..8174)
                     /locus_tag="PLL59_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23932.1"
                     /translation="MIIVVKHRIANPEKFWASAQESLPKLPENGVSRVLQVLPNANAT
                     EATCLWEAESIASLDAYLRAKVYDWSAEEYQEVNTAQSMGLSL"
     gene            8334..9947
                     /locus_tag="PLL59_08730"
     CDS             8334..9947
                     /locus_tag="PLL59_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HMY23933.1"
                     /translation="MEFDYIIIGGGSAGCVLANRLSENANHTVCLLEAGPPDDSHFIH
                     NCNPVNMLFLMNSPKYNWCYYAEGNTKTGNRKFYWPRGKTLGGSSAVNAMIYTRGHPS
                     DYDHWESLGNKGWNFQAILPYFKKSELQHRGEDAYHSKEGTMDVIDPTFHFPPSHAFV
                     KACAEAGIPHNNDINGAKYEGVDFFQVNQTPDGRRCHSSVSFLNPVRDRKNLTIINYA
                     HIKRVVFEGTTATQVEYFDLKDNKKIKSIAANKEIILSAGIIGSPQILKLSGVGPKAE
                     LEKHGIPVVKDLPGVGENMQDHPDIIIRCRDKSGSALTTFPWPYMVKFMSKFYRKNKA
                     PFIFTPTDAGGYVKSDPSVEVPDLQLQFASVRMLPHGSGLFTPTKSGFVLHICHMRPK
                     SRGNVLLKSADPFDAPIINANYFDDEMELDALVKGVKIARNIIAQDAFKPYFGSEEVP
                     GKHVQSDAEIRQFILDKAESVYHTAGSCKMGIDEMAVVDPTLKVYGVKNLRVIDSSIM
                     PTITGSNIHAPTVMIAEKGADMILNENSK"
     gene            complement(10112..11251)
                     /locus_tag="PLL59_08735"
     CDS             complement(10112..11251)
                     /locus_tag="PLL59_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23934.1"
                     /translation="MKSFAEIDIEKNGNYLKLLSAVSKLSGLFSESAIPFINYRVAEN
                     IFCRSFDASNLSRSDTAFDANYNSVGVGLKTFTCNTYNSTEKVAEFNSLSRVLAEFKG
                     NELAIKLGEFRNDRINLANRLYNIESSLYHIVARKENELLLYETDYNIIDIANIHSVK
                     DNKASLQFEDGNNLYSFNYSKSTLFRKFIIPQNAFRLPIDIIEDPYSLLLELFEENKN
                     LKTATDKLIKGENYVILPLYGIQKKQKFIFERSGLNQWNANGRKRDFGEIYIPIPAEL
                     HRKYPTFFPKRDEDFSLQIPTGEIFSAKVCQENSKALMTNPNKALSDWLLRKVLQLKE
                     GELATMEKLDNLGFDSVIITKSDNQNFKIDIMKTNTYNEFNQDEE"
BASE COUNT         3182 a         2216 c         1862 g         4133 t
ORIGIN      
        1 cctttcattt tcattttgtg aactcttcta aattttactc ttttcggttg taacataacc
       61 ctatttattt gagattttag atttcagatt tcagatttgt attcctattt cctacttctt
      121 taataattat ttttttcttg gtcctctatt ttcagatctt tcacctcttg gttttccgcc
      181 gccacgtttg tcgccaccac cacgacgatc atctcttctc tcaccacctc ttctgtcatc
      241 gcgtctctca ccaccttttt tattagcttc ttccatgcca atatttggag aaaggtcgcg
      301 ttttgtgaac acttcgcctt tacaaatcca aactttcaca ccaatttttc catagatagt
      361 ttccgcttct ttccaagcat aatcgatatc tgctctaaat gtatgtaatg gtgtacgtcc
      421 ttctttgtac tcttcacttc ttgccatctc tgcaccacct aaacggccag agcattttat
      481 tttgatacct tcaacaccta ggcgcattgc agatgcgata gccattttta cagcacgacg
      541 gaagtttaca cgagcttcta actgtttgca aactgtttca cccacgatag cagcatcaat
      601 ctcaggtctt ctaatttcaa cgatattgat ttgaacatct tttttcgtga tttttttgat
      661 ttcctctttg attttatcta cttcatttcc accttttccg ataatgatgc caggacgaga
      721 tgtattaatt gaaatcgtga ttctattaag tgttctttcg atgataattt tagaaatacc
      781 acctttttga atacgcgcgt ttaagtattt tctgattttt tgatcttcaa ttaatttatc
      841 gctaaaatct ttgccgccat accaattaga gtcccatcct ctgatgatac ctaatctatt
      901 tgctatcgga tttgctttag tacccatttt atttttttag attatttttc gttattttca
      961 gttgtttgtt ctgcttgcgc tacgcggcta gccaattgca ctgtaatgtg gttagagcgt
     1021 ttacgaattc tgtatgctct accttgaggt gcaggttgga atcgttttag aattcttgca
     1081 ctatcaatca ttactgattt tatgtacaat tcgctatctt ctgctttcgt ttcgaacttt
     1141 tgttcccagt ttgcaatggc agagcgaact aatttttcca ttgttttaga agcatgtttg
     1201 gttgagaatg taaggatatt taatgcttcc attacatctt tttcacggat tacgtctgcc
     1261 aacaatctca ttttacgagg tgatgtaggc acatttgtag cacgtgcgat acaaactgat
     1321 tttctagctt cttttctagc gtctgctgct attttttttc tttgacccat ggcttaattc
     1381 ttattttctg ttacctgaat gtcctttaaa ttgtctagtc ggtgaaaact ctccaagttt
     1441 atgacctacc atattttctg ttacatatac aggaatgaat ttatttccgt tgtgtactgc
     1501 aaatgtatgt cctacgaatt ctggagtaat catagaggca cgagaccatg ttttgattac
     1561 tgttttttta ttagatgcat tcatagcatc aactttacct tgtaagtggt atgctacgta
     1621 aggacctttt tttaaacttc ttgccattct ctatctatta tttttttgct cttgaaataa
     1681 tgtgtttgtt cgatgctttg gttttacttc ttgtttttaa acctttagaa ggaatacctt
     1741 ttcttgaacg agggtgacca cctgaagctc taccttcacc accacccatt ggatggtcaa
     1801 ctgggttcat tactactgca cgcgtacgag gccttctgcc tgcccaacga tttgcgcctg
     1861 ctttaccttt tacctctaag ctatggtcag agtttgaaac agaaccgata gtcgctaagc
     1921 aagtacctaa gatttgtcta gcttcgccag aaggtaattt gatatttaca tacttaccat
     1981 ctttaccaga taattgggca aatgtaccag cgcttctgca aattgaagct ccacgacctg
     2041 gttgcaactc gatattatga ataactgaac ctaatggcat attagacaat ggtaatgtat
     2101 tacctacttc tggtgctact gatttaccag aaataatttc ttgaccaact agaatacctt
     2161 caggtgcaat gatataacgt ttttcaccat ctttatatac tactaatgcg ataaaagcag
     2221 agcggtttgg atcgtattcg atagttttta ccgttgctgg tatatcgaat ttattacgtt
     2281 taaaatcgat gatacggtat tgtcttttat gaccaccacc aatatagcgc attgtcattt
     2341 ctcctttttg gttacggccg ccagattttt tattagaaac aactaatgat ttctctggta
     2401 cgttggttgt cacctcacta taagagttcg atattctgta tcgtaaactc ggcgttatcg
     2461 gattgtattt tttagttgac attacttatt ctattttaaa attatatttc gttgtagaaa
     2521 tcaattactt caccttttgc aactttaacg attgcttttt tataacttgg ttttctacca
     2581 gaaattactt gcgtttttgt attgcgattt ttaggtttag aagcaataat tgctgtattt
     2641 acttcttcta cattaacgcc gtacattttc tcgatcgctt ttttaatttc tactttgttt
     2701 gccttttttt ctactaaaaa agtgtacttg cctgcttttt ctgatagtgc agatgctttt
     2761 tcggtgatta aaggtttttt cagaatttcc attctattta tttttatgaa tttgctaata
     2821 cttcaattga tttttcgcta atcaagatag tgctgttgtt catgatttcg taagtattta
     2881 agctatccgc agtagcaata tttgctcctt gtacgtttct gctagataaa tacacgttat
     2941 catttaattc tggaagaatt aataaagact ttttgttttc taagccaaaa gcttgtaaga
     3001 attttaaata ttcttttgtt tttggtgcat tgaatgtaaa gtcttcaact attttgattg
     3061 cgctatctgc tgctttcact gacaaagcag aatttttcgc taatgatttt acttttttat
     3121 ttaatttaaa atcgtaaaaa cgaggtcgtg gaccaaatat acgagcaccg cctttaaaca
     3181 aaggattttt gatgctacct tttctcgagc cacctgttcc tttttgttta tgcaactttc
     3241 tagtagaacc agataattct gatttttctt ttgttttatg cgtaccgctt cttcttcctg
     3301 ctaaatattg cttaacagct aagtagataa catgctcatt aggttcaaca ccaaaaattg
     3361 ctgctggcaa ctcgatgcta cgtcctgtgg cttgtccgtt aatattatat acttctgctt
     3421 tcatctttta tgatttaccc ttagtaagag aaactgattt tttacttttt tcaattataa
     3481 cataagctcc attatgacca ggaattgatc ctttaacgat aattaaattt tcgtcgttca
     3541 ataccttcac aattttcatg tttagttctt tcactctgtc agtacccatg cgaccaccca
     3601 ttttcatgcc tttcattaca cgtgctggtg tagaacaagc acctaatgaa cctggcgcac
     3661 gcaaacgatt gtgctgacca tgagtttgac cacctacacc gctaaatccg tgacgtttta
     3721 caacaccttg gaaaccttta cctttagatg tgccaatgat atttactttt tcgccttctg
     3781 ctaaaatctc agatgtaacg atatcgccaa catttaaatc atccatgcct ttgaattcta
     3841 ctaattttgc ttttggagcg ccaccagatt tttcaaagtg gtttttcatt ggtttagtgg
     3901 tatttttttc ttttttatca ccgtaaccta attgtattgc atcataacca tctttatctt
     3961 tcgatttttt ttgtgttaca acacatggac cagcttgaat aactgtgcat ggaataattg
     4021 caccgccttc gccgaaaata ctggtcatac ctacttttgt tcctattaat cctgccatga
     4081 cttattacac tttaatttct acatcaacac cagatggtaa ttctaatttc attaatgcat
     4141 ctacagtttt aggtgttgcg ctatagatgt ccaataatct cttgtatgta cttaatttga
     4201 attgctcacg cgcaacttta tttacgtgtg gtgatttcaa aacagtaaaa atctttttgt
     4261 tggtaggcaa tggaattgga ccagtgacta cggctccagc actgcgtact gtttttacga
     4321 ttttttcagc agacttatct actaagttgt gatcgtatga ttttaatttt attctaattt
     4381 tttgtgccat gatatatatt atctattaat cagcttttac tttagatttt gcttttgcta
     4441 ctacttgttc tgtaatgtta tttggtgttg gtgcgtaatg agagaattcc atgatagatg
     4501 ttgctctacc agatgtaatt gttctcaagt ctgttacata accaaacatt tctgacaatg
     4561 gaacttttgc tttaataacg tttgcattat ttttcatctc catgccttcc atcataccac
     4621 gacgacggtt taagtctcct accacatctc ctgtattttc ttctggtgtg attacttcta
     4681 atttcattat tggctccaac aagattgggt ttgcctttct gcaagcttca cggaacgcaa
     4741 acttagccgc ttgttcaaac gagaatgagt cagagtccac ttgatggtat gaaccatcaa
     4801 ataagcgtac ttttaagctt ggtacttcgt aacccgctaa tacaccagtt ttcattgcct
     4861 ctttgaaacc tttttctaca gatggaataa attcttttgg aattgctcca ccaaagatat
     4921 cattgataaa ctctaatcct tctttatctt ctgcaccagg tccgatttcg atttgaatat
     4981 cagcaaactt accacgacca ccagattgtt ttgcatattt ctctctgtgt tcaatgctgt
     5041 ttgtgatagt ttctttgtag ttaacttgtg gagcaccttg attacattca actttgaact
     5101 cgcgcaataa acggtcaacg atgatttcta agtgcaactc acccatacct gagataactg
     5161 tttgtgcagt atcttcgtca tatctcactt taaatgtagg atcttcttcc gctaatttag
     5221 ccaaagccat acctaattta tcagcatctt tttgtgtttt aggctcaata gctaaaccga
     5281 taaccggatc agggaatacc atagactcta acacgattgg atttccttct gcacataaag
     5341 tatcaccagt tctgatgtct ttaaaaccaa caactgcacc aatatcacca gcacctaatt
     5401 tttcgatagg gttttgttta ttcgcatgca tttggaaaat acgagaaata cgttctttat
     5461 ttccgcttct tgtattgatt acataagaac cagcatctaa aacaccagag taagcacgtg
     5521 taaatgctaa acgacctacg taagggtcag tagcaatttt gaatgctaaa ccagcaaatg
     5581 gttcgtcgta gcttggctta cgcactacct cttcgcctgt atcaggatgc gtacctttaa
     5641 tatttggtct gtctaatggt gatggcatta attccataac caaatccaac atagtttgta
     5701 cacctttatc tttaaatgaa gaaccgcaca acattggaac gattttcata tctaagcaag
     5761 cagaacgcaa ggcatctaaa atttctcttt ctgagattgt agaaggatca tcgaagaatt
     5821 tctccatcaa tttgtcatca tattcagaaa ctgcttctaa taaacgctct ctccacattg
     5881 tagcttcttc taccatttcc gctggaatat ctaccacttc atacgtcata cctctatcgt
     5941 gctcattcca aatgattcca cggaagttaa ttaaatcaac tacgccttta aatgtatctt
     6001 cagcaccgat tggcaattgc aatggaacag catttgaacc taacattttt ttcacttgct
     6061 ccactacatt caagaagtca gcacctgagc ggtccatttt atttacgaaa cctaaacgag
     6121 caacgttata attgttagct aatcgccagt ttgtttcaga ttgtggctca acaccatcaa
     6181 cggctgaaaa caagaaaact aaaccatcta atacacgcaa tgaacggttt acctcaacag
     6241 taaagtccac gtgtcctggt gtgtcaatca agttaacatg gtactcttga cctctgtagt
     6301 tccattttac tgtagttgca gcagatgtga ttgtgatacc acgttccgct tcttgcacca
     6361 tccagtccat agtagaagca ccatcatgca cctcacctat tttatggttt acaccagcat
     6421 aaaacaatat tcgctcagat gtagtagttt tacctgcatc aatgtgagct gcaataccaa
     6481 tatttcttgt aaattttaaa tcgcgtgcca tttttttctt ttaattgaca catttaatgt
     6541 gtctctcttt ttttttattt tgacacatta agtgtcgtta ttattattat gctttaaaat
     6601 gtgagaatgc tttattcgct tctgccattt tgtgtgtatc ctcttttctt ttcactgcgg
     6661 caccttctcc ttttgatgct gcaattaatt ctgctgctaa tttatcgccc attgattttc
     6721 cgtttctttt tcttgaaaat tgaatcaacc aacggattgt cataaataat tttctgtctt
     6781 cacgaattgg tgttggaatt tggaaagttg caccaccaat tctgcggctt ttcacctcta
     6841 cagctggcat ggcattatcc attgcttttt tccagatttc gtatccttct tcttttgtag
     6901 aagctttaat acgttccatt gcttcataaa atgcagtata agctgtactt tttttaccgc
     6961 caatcattaa gttattcaca aaacgagtta ctaaaggatc tccataaact ggatccggtg
     7021 ctaagtatct tttttttgct ttttgctttc tcatttattt tatttttaag tcagacacat
     7081 tataaatgtg tccacacatt attttttacc ttttcctttt gctggtgctg ctgcgcctgc
     7141 tttacctttt ttagttccgt atttagagcg gcttttttga cgatcgctta ctcctgctgt
     7201 atctaatgca cctcttacga tgtggtaacg aacacctggt aaatccttaa cacgaccacc
     7261 acgaatcatt acgattgagt gctcttgcaa attgtgtttc tcacctggaa tataagcgat
     7321 aacttcaatt ttgttggtta aacgcacttt tgctacttta cgtaaagctg aatttggttt
     7381 cttaggtgta gttgtataaa cacgtgtaca tacaccgcga cgttgagggc aagcgtctaa
     7441 tgctcttgat ttagaagcat attgaagttg ctctcttcct tttcttacta attgttgaat
     7501 tgtaggcatt cgttaaaaac gttattttta aaaatttcgg actgcaaagg tagggaaaga
     7561 agtcaagatt accaaacata actgtaaaga agaagttatg ttttttgtag tcttaccttc
     7621 cctgcgaagg cagttactac aagaagaaat ttctgataca atcggatttt cctcctgaag
     7681 tgggcgcaaa gatacaaatt aattcgcaat tgtggaaaat tattctgtat taatttcgag
     7741 ttaagaatga atttgtgagg taaaatgcga taaacaataa tttattagcg cataaacaat
     7801 tgattatcaa caaaatattt cttttgattt gtgttgacct tatgtaattc caataaaaaa
     7861 tgggctgctt gatggcagcc cagaaacttg taacgaatga atttacaatt ttacagcgac
     7921 agacccattg attgagccgt atttacttct tgatattctt cagcactcca atcgtaaact
     7981 ttagctctaa gataggcatc taatgatgcg atactttcgg cttcccagag acaggttgcc
     8041 tctgtggcat tggcgtttgg cagtacttgc aatactctcg acacgccatt ttctggcaat
     8101 ttaggtaaac tttcttgtgc acttgcccaa aatttttctg ggttggcgat tctgtgtttt
     8161 actacaataa tcattgctaa tggttttgag tgataaataa taaagataaa tgtagcacat
     8221 tcttttcaat taacatctgt aaaaaagtaa acctctgcct tttttttgat tcgtattaca
     8281 taaatctttt gcttaattgt tcctattttg ttttatttac attcaaaaac tacatggaat
     8341 ttgattatat cattattgga ggcggatcgg ctggttgcgt attagcaaac agattatcag
     8401 aaaacgcaaa tcatactgtt tgcttgctcg aagctgggcc acctgatgat tcacatttta
     8461 tacataattg caatccagta aatatgttat tcttaatgaa tagtcctaaa tacaattggt
     8521 gctattatgc ggaaggaaat acgaaaactg gaaataggaa attttattgg ccgagaggca
     8581 aaacattagg tggttcgagt gctgtaaatg caatgattta tacacgtggg catccttcgg
     8641 actatgacca ttgggaatca ttgggaaata aaggctggaa tttccaagct atattgccat
     8701 attttaaaaa atcggaatta caacacagag gcgaagatgc ataccatagt aaagaaggca
     8761 cgatggatgt tatcgatcct acttttcact ttccgccaag tcatgcattt gtaaaagcgt
     8821 gtgcagaggc tggcattccg cataataatg acattaatgg tgcgaaatat gaaggagttg
     8881 atttcttcca agtaaaccaa acaccagacg gcagacgctg tcactcatct gtctcatttt
     8941 taaatccggt gcgcgacaga aaaaatctaa ctattatcaa ttatgcacac attaaaagag
     9001 tggtatttga aggcacaaca gctactcagg tggagtattt tgacctaaag gacaataaaa
     9061 aaataaaaag tatcgctgcc aataaagaga ttatactcag tgctggcatc attggttcgc
     9121 cacaaatcct caaactttct ggtgttggtc caaaagcaga attggaaaaa catggaattc
     9181 cggtagtgaa ggatttgcct ggtgttggtg aaaacatgca agatcatcct gatatcatta
     9241 ttagatgtag agacaaatcc ggttcagcat tgaccacatt tccatggcct tacatggtaa
     9301 agtttatgtc aaaattttat cgaaaaaaca aagcaccatt tatatttacg ccaactgatg
     9361 caggtggtta tgtaaaatca gatccaagcg tagaagttcc agatttgcaa ttgcaatttg
     9421 catcggttcg tatgttgcca catggcagtg gtttatttac gccaacaaaa tcaggatttg
     9481 tattacatat ttgccacatg cgtccaaaaa gcagaggaaa tgtgttatta aaatcagctg
     9541 atccatttga tgcgccgatt attaatgcga attattttga tgatgaaatg gaattagatg
     9601 cattggtgaa aggtgtaaaa atcgcaagaa acatcattgc gcaagatgcc tttaagccat
     9661 actttggtag cgaagaagta ccaggcaaac acgtacagtc tgatgctgaa atacgccaat
     9721 ttattctaga caaagcagaa tcggtgtatc atactgctgg ctcttgtaaa atgggcatcg
     9781 acgaaatggc tgtggtggat cctacgttaa aagtatatgg cgtgaaaaac ttacgagtta
     9841 tcgattcatc tatcatgcct acgattacag gcagtaatat tcatgcgcct accgtaatga
     9901 ttgccgaaaa aggagctgat atgatactaa acgaaaattc taaataaatt tggaaacaaa
     9961 aaaatgcaat taaatgctaa cagtttgcag cttggcaaat agtggcattt tataacaaaa
    10021 gttcaataga attactactg ttcaatcttg catttctgct caaagtagcg cactctcgac
    10081 acaatgccta actgtatgtt agctatagtg cctattcttc gtcttgatta aattcgttat
    10141 atgtattcgt tttcattatg tcaattttga aattttggtt gtcagatttt gtaataatta
    10201 cgctgtcaaa accaaggttg tctaatttct ccattgtcgc caattcccct tcttttagtt
    10261 gtaatacttt tcttaaaagc cagtcagaaa gtgctttgtt cggatttgtc attaaagctt
    10321 ttgagttttc ctgacaaact tttgcactaa atatttctcc tgttggaatc tgtaaactaa
    10381 aatcttcatc tcttttcgga aaaaatgttg gatattttct atgtaattcc gcaggaattg
    10441 gaatatatat ttctccaaaa tctcttttcc ttccattcgc attccattga tttaacccgc
    10501 tacgttcaaa aataaacttc tgctttttct gaattccgta aagtggtaaa atcacgtaat
    10561 tttcgccttt tataagtttg tcggttgcag tttttaaatt tttgttttct tcaaaaagtt
    10621 caagcaacaa agaatatggg tcttcaatta tgtcaattgg taaacgaaac gcattttgtg
    10681 gtatgatgaa ctttctaaaa agtgtgcttt ttgaataatt gaaagaatac aaattgtttc
    10741 cgtcttcaaa ttgtaaactt gctttgttgt cttttacaga atgaatattc gcaatatcaa
    10801 tgatattata atcagtttca taaagtaaaa gttcattctc ttttctcgca acaatatgat
    10861 aaagtgaact ttctatatta taaagtctgt tggctaaatt tattctatcg tttctaaatt
    10921 ctcccagttt tattgccaat tcattgcctt taaattcggc tagaactctt gaaagtgaat
    10981 taaattcagc tactttttct gtactgttgt aagtgttaca agtaaaagtt tttaagccaa
    11041 ctccaacaga attataattt gcatcaaaag ccgtgtcgga acgagaaaga ttactagcat
    11101 caaaacttct acaaaaaata ttttctgcaa cacgataatt aatgaaagga attgcacttt
    11161 cgctgaacaa accagaaagc tttgaaactg ctgaaagcag tttcaaataa tttccgtttt
    11221 tttctatgtc aatttctgca aatgatttca ttaatcaact cttttagata gatgatatag
    11281 ttctccttta tcatctgtca taacttcaag aatgcgagtg taatttttta tttttgttgc
    11341 tttctcaaat tcaatttata aagaactttc caaattgctt aaataatcat tat
//
LOCUS       DAOQQV010000080        11321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2037_length_11321_cov_7.536836, whole genome shotgun sequence.
ACCESSION   DAOQQV010000080 DAOQQV010000000
VERSION     DAOQQV010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2037_length_11321_cov_7.536836"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /gene="sucC"
                     /locus_tag="PLL59_08740"
     CDS             <1..544
                     /gene="sucC"
                     /locus_tag="PLL59_08740"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HMY23935.1"
                     /translation="KIIAVDAKVNLDDNALFRHQDLEAQRDLLEEDAIEVEAAAAELN
                     YVKLDGNVGCMVNGAGLAMATMDIIKLSGGEPANFLDVGGTANAERVEKAFRIIMKDE
                     HVKAILINIFGGIVRCDRVAEGVIEAYKNIGNIEIPIIVRLQGTNADIAKKMIEDSGL
                     KVQSAILLSEAADLVAKAVA"
     gene            669..1193
                     /locus_tag="PLL59_08745"
     CDS             669..1193
                     /locus_tag="PLL59_08745"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HMY23936.1"
                     /translation="MQLNMLLSSLIASIIPLLVGFIWYNPKVFGTAWMNACGLEESKL
                     QEGFNMPLVFGLCILFGFFISFGISSVVIHQFGFFGMLQHQVEIKNPEALSFLQSGFE
                     KYGNEFRTFKHGALHGTILGITVALPIVGIGALFERRGWKYIAINAGYWILTLALMGG
                     FICQFSKVSLEMFQ"
     gene            1274..1888
                     /locus_tag="PLL59_08750"
     CDS             1274..1888
                     /locus_tag="PLL59_08750"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMY23937.1"
                     /translation="MLLELFPHNIDMRKMKQVVDCLRNGGLVIYPTDTVYGLGCDIFN
                     KSAVEKICRLKGVKLEKMNFSFICYDLSHISDYTLNVDTRTYKLMKRCLPGPFTFILK
                     ANNAIPKLFKNNKKTVGIRVPDNDIARMIVQELGNPIITTSVHHEDDIIDYITDPNAI
                     YERYEKIVDIVIDGGIGGYKPSTIIDCTGEEPIIVRDGIGKIDL"
     gene            complement(1910..2452)
                     /locus_tag="PLL59_08755"
     CDS             complement(1910..2452)
                     /locus_tag="PLL59_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23938.1"
                     /translation="MKINKIIGLTTIAFVVFFSSCKLIDKLTKFDVNYSTNYTIPASL
                     VPGILGDIFTPDITTNSEQIFTNNNTKADLLEQVNLKSLQLTITSPSGKTFDFLKSAS
                     VFVSADGLPEVKIAYIDNIDDSVGNSISLNATGVELKEYLKKSKIKIRVANTTDKIVS
                     ENVTIKIDAVFFVDAKILGV"
     gene            complement(2513..3070)
                     /locus_tag="PLL59_08760"
     CDS             complement(2513..3070)
                     /locus_tag="PLL59_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMY23939.1"
                     /translation="MLGFINGLIVSNAFEWYFHKHVLHGQGKKKDSFWRFHWAVHHKT
                     VLKEEFRDSDYEHNILDAWDPQSKEVAALVFGAVAVSPLFWIAPGFTAGVWFSQYNYY
                     RVHKKSHLDPEWAYKYLPWHYDHHMAPDQDKNWCVTFPLWDYVMGTRLHYKGTEREKI
                     DIERRMRKAAVTTNKQVFSKTSENK"
     gene            3181..4353
                     /locus_tag="PLL59_08765"
     CDS             3181..4353
                     /locus_tag="PLL59_08765"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HMY23940.1"
                     /translation="MQVQTIRLANSISIQIFEHISAIDEQVWNSITTNIFFYQTHQFL
                     SVIEEIHTTIQFRYVLVYKEDKIVAGLYTQLLHFSFRNLVNYNANASTGIKSKVKQYI
                     AKKDTKLLNLGNVFFTGDKGIICQQDDNIISLLPDIFTKLYASFTKQKPRAYLVANIY
                     LQDESKCASFSSHAFHPFITEPDMLMPIAAAWTSFDDYLSAISSKYRVRDKKILALSS
                     SIKTVNFTLEEIKNNRVHFSHLYANVINHVAFNMATLSVDFFERMKLLYVDKFNIIGY
                     YKEEALVGFACLFNVDENMLHVHYIGLDYTINKEQKLYNRMLLDFVKFAIENKKNKIH
                     FGRTATEIKSTIGAQAFPLNAYLKMHNPMLNISLPYFLSRIKPAEYTLRNPFRELS"
     gene            complement(4350..4961)
                     /locus_tag="PLL59_08770"
     CDS             complement(4350..4961)
                     /locus_tag="PLL59_08770"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMY23941.1"
                     /translation="MRKAILIPILFTCLMWTLFIIHFFTNVNFSYLGIHPRDSHTLIG
                     IFTAPLIHGNWEHLISNTLPILILGSILLYMNKKIGILIFICNYLITGLLVWCFARGN
                     SYHIGASGIIYGMASFLLFNGFFRMDIKSIAIASGVALFYGGMVWGIIPEQAGISWES
                     HLAGSFSGFVLAFIFRNWHKDDLYKLQENETMQRKTFEEYINR"
     gene            5120..6220
                     /locus_tag="PLL59_08775"
     CDS             5120..6220
                     /locus_tag="PLL59_08775"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HMY23942.1"
                     /translation="MIIGIPKETKFKENRVAITPLIAKDLIKQGFTVHVESGAGLNSF
                     FSDDNYKEAGATLSDKNTTYQADIVLKVNAPLPEEIALMKKDAVLISFMWAATNPELV
                     SACEKAGISAFSMDAVPRISRAQKMDALSSQANLAGYKAVIMCADTLGKIFPMMTTAA
                     GTIKPAKVVIFGAGVAGLQAIATAKRLGAIVEVSDIRPETKEQVQSLGGKFIEVKGDD
                     TIKMEGGYVKGVSDEFLKKQQELVGKHVAEADIVITTALIPGRKAPVLVTEEMVKSMK
                     FGSVILDMAVEQGGNVVGSKLNENVNINGVTIIGEGNIPSLLPMNASDLYAKNIQTLL
                     YHLATKDGFKWEMEEEITKGSLIVHQGKNVKA"
     gene            6262..6564
                     /locus_tag="PLL59_08780"
     CDS             6262..6564
                     /locus_tag="PLL59_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HMY23943.1"
                     /translation="MEILNFFVENREMIYFIVLSIFVGIEVIGGVPTVLHTPLMSGAN
                     AIHGVVIVGAIIVMLDTEPNNILGLILGFLAVILGTLNVVGGFVVTDRMLEMFKKK"
     gene            6620..7003
                     /locus_tag="PLL59_08785"
     CDS             6620..7003
                     /locus_tag="PLL59_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMY23944.1"
                     /translation="MDKTTKITSYKDLIVWQKSIDFVSYIYGITSQFPSAEKFNLTSQ
                     INRAVISIPVNIAEGFGRESSKNYIQFLKISRGSLYEVDTLFIISKNLEYITTEVYSQ
                     TTLFINEISKLLNGLIKSIQNKIIN"
     gene            7052..8470
                     /locus_tag="PLL59_08790"
     CDS             7052..8470
                     /locus_tag="PLL59_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HMY23945.1"
                     /translation="MKGILEISYLIGSITFIVGLKMMGNAKTARKGNLIGAFGMTVAI
                     LGTIFLYEKEGQTSREFFKIALIFGAIAIGTAIGWITAKKVEMTKMPELVSMFNGMGG
                     ACAALIGLTEFPHVAHQIQETGTGYSSMAFIIAGLVIGTISFSGSIIAYLKLAGKMNK
                     PIRLPQYNILNNALLLVVIVFSAYVVLQSTSLPPAIVWALFVVAIVYGILFTIPIGGA
                     DMPVVISLLNSFTGLAAAFGGFLYDNKPMLTGGILVGSAGTLLTLAMCKAMNRPLSNV
                     IFGAFGGGSGAAGADAGEKGTVKDVQVSDLAVQLNYASKVVIVPGYGLAVAQAQHVIH
                     ELEKILEERGVEVKYAIHPVAGRMPGHMNVLLAESNVEYDKLVEMEDINPQFAQTDVV
                     LVVGANDVVNPAAKVDPSSPIYGMPILEVENAKNIVINKRSMNAGYAGIDNLLFYNPK
                     TSMFFGDAKKALTELVAEIKQL"
     gene            complement(8526..8906)
                     /locus_tag="PLL59_08795"
     CDS             complement(8526..8906)
                     /locus_tag="PLL59_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23946.1"
                     /translation="MNSVEIILYVSDQNASTKFYEKLLDKKPVLFVPGMTSFQIVENC
                     RLGLMPSNSIAKILQNKTPHPDNGSGIPRCELYVYVDDIHQAYKRAIDCGASLISDIA
                     LRDWGDYACYFADCDGHIIAFAKK"
     gene            complement(8926..9609)
                     /locus_tag="PLL59_08800"
     CDS             complement(8926..9609)
                     /locus_tag="PLL59_08800"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HMY23947.1"
                     /translation="MKKIVLIISLFICTQAFSSDYLKVLYVADQYHTIGTNKYLMTRE
                     TPTDTFSIFYENIAGFTYETGYTYCLLTTVSKNTDTSTNITTYQYSLSEIKSKIKTQN
                     SNLETNVKKIYTVEDSSIWLLYKLRMKDGSTKTFSLQKAYIRFDIKNNTISGNSDCNE
                     LSASFTMDSSSILFENISTTKMKCGKHAIEPIFIQMLQQATNYRIKNKQLLLFKGKSM
                     LGLFTRKKD"
     gene            9655..10092
                     /locus_tag="PLL59_08805"
     CDS             9655..10092
                     /locus_tag="PLL59_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HMY23948.1"
                     /translation="MKKKILLVLAIVLIVIQFIHPTKNQQANILDSDITKVRSVPEDV
                     QQLLKTACYDCHSNNTVYPWYNNIQPVAWWLNKHVNNGKKQLNFSEFGAYDEKKAKHK
                     LHELVETLEKNEMPLFSYTIIHKDAKLNDAQKKIIIDWAKANQ"
     gene            complement(10138..11112)
                     /locus_tag="PLL59_08810"
     CDS             complement(10138..11112)
                     /locus_tag="PLL59_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMY23949.1"
                     /translation="MHSIEELRNIFLAYLQTENKIIEPKNLYEPNHYFLQIGGKRLRP
                     ILLLLATDIFDGEIEQSLAASLAIEYFHNFTLIHDDVMDKAPLRRGFTTIHEKYNLNT
                     AILSGDVLMIKACEYLAKVNEKYFKTVFDVYTKTAIEVCEGQQYDIDFETKEVVTHDE
                     YIEMIRLKTSVLLAASMKIGAILGGAKKEDTELLYQYAENLGIAFQIQDDILDCYGDE
                     KLVGKQPGGDIIQNKKTLLLIHAIEKSNANNDTRLNEILKQSVFNNDEKVNDILAIFN
                     TYNTKAFGIEKRDMYIQKSIDALEKINLNSEKKAVLNSLIDYLVMRDF"
BASE COUNT         3779 a         1754 c         1960 g         3828 t
ORIGIN      
        1 taaaattatt gctgttgatg caaaagtaaa cttagacgat aacgcattat tccgccatca
       61 agatttagaa gcgcaacgcg acttattaga agaagatgct atcgaagtag aagctgcggc
      121 agctgaattg aattacgtga aattagatgg caatgttggt tgtatggtaa acggcgcagg
      181 tttagcaatg gcgactatgg atattattaa actttctggt ggcgagccag caaatttctt
      241 agatgttggc ggcacagcaa atgccgaacg tgttgaaaaa gcttttcgta ttattatgaa
      301 agatgaacac gtaaaagcaa ttctaatcaa tatatttggt ggtattgttc gttgtgatcg
      361 cgtggcagaa ggtgtaatag aagcttataa aaacatcggt aatatcgaaa ttccaattat
      421 cgttcgtttg caaggcacga atgctgatat tgcaaagaaa atgattgaag actctggttt
      481 aaaagttcaa tcggctatct tgctttctga agcagcagat ttagtcgcta aagcagtagc
      541 ataaatagtt tcaatatatt ttacagagcc actttttaca agtggctttt ttttgttgat
      601 aattaattta ttttcaattt gaccttaata ctgaaatatc gtacattgaa tgaataaaaa
      661 taaaagctat gcaattaaac atgttactgt cgtcgttaat cgcgtctatt attcccttat
      721 tagtcggttt tatttggtac aatccaaaag tatttggaac ggcttggatg aatgcctgcg
      781 gacttgagga atcaaaatta caagaaggtt tcaatatgcc attggttttt ggcttgtgta
      841 ttctttttgg tttttttatt tcatttggaa tttcaagtgt tgttattcat caattcggat
      901 tttttggtat gttacaacat caagtagaga ttaaaaatcc tgaagcatta agctttctcc
      961 aatctggctt tgaaaagtat ggaaatgaat ttcgtacttt taagcatggc gcattacacg
     1021 gcaccatctt aggtattaca gtagccttgc ctatagtggg cattggagca ttgtttgaaa
     1081 gacgtggttg gaaatacatc gccattaacg ctggatattg gattcttacc ttggctttga
     1141 tgggtggctt tatttgtcag ttctcaaaag tcagtttgga aatgtttcaa taaaaataca
     1201 aagtcgccgc aaggcgactt ttttattatt tatgcttacc ttacagcaac atacatttaa
     1261 taaatagcat cttatgctgt tagaactatt tccacataat atcgatatgc gcaaaatgaa
     1321 acaagtagtg gattgtttgc gcaatggtgg attagtgata tatccgacag atactgtgta
     1381 tggcttaggc tgcgatattt ttaataaaag tgctgtcgaa aaaatctgta ggctaaaagg
     1441 tgtgaagttg gaaaaaatga acttctcttt tatttgttac gatttaagtc atattagcga
     1501 ttatacgctg aacgtagata cacgcacata taaattgatg aaacgctgtt tgcctggtcc
     1561 atttactttt attttaaaag cgaataatgc aattcctaaa ttatttaaaa ataataaaaa
     1621 aacagtcggt attcgcgtgc ctgataatga cattgcaaga atgatagtgc aggagctagg
     1681 caatccaatt attacaacat cagtacatca tgaagatgat atcattgatt atatcactga
     1741 cccaaatgcc atatatgaac gatatgaaaa aatagtggat atagtgattg atggtggaat
     1801 aggtggctat aaaccatcaa caattattga ctgcacaggc gaggagccaa taattgtgag
     1861 agatggtatt ggtaaaattg atttatagat tatagataaa agcctttgat taaacgccca
     1921 atattttagc atctacaaag aatacagcat ctatctttat agtcacattt tcagaaacaa
     1981 tcttatctgt tgtgttggca actctaattt ttatcttaga ttttttgaga tattctttta
     2041 attcaacacc tgttgcgttt aatgaaatac tattgccaac gctatcgtcg atattatcga
     2101 tgtaagcaat ttttacttca ggcaagccat cagcactgac aaatacactc gctgatttca
     2161 agaaatcaaa tgtttttcca cttggacttg taattgtaag ttgtaaactt tttagattta
     2221 cttgttctaa taagtctgct ttcgtgttgt tattggtaaa tatttgttca gaatttgttg
     2281 taatatctgg cgtgaaaata tcacctaata ttccaggaac taagctggca ggaatagtgt
     2341 aatttgtgct gtaattaaca tcaaattttg ttagcttatc gataagtttg caactactaa
     2401 aaaacacgac gaatgctatt gtcgtgagac caataatttt gttgattttc ataaagtttg
     2461 tttacgttgc tacaaagtaa attttttagc attgtaatgt caagctttaa ccttatttat
     2521 tttctgaagt tttagaaaat acttgtttgt tggtagttac agctgctttt cgcattctgc
     2581 gttctatgtc tattttttca cgctctgttc ctttgtaatg taaacgtgtg cccattacat
     2641 aatcccaaag tggaaatgtg acacaccaat ttttatcttg gtcaggagcc atatgatggt
     2701 cataatgcca aggcaaatat ttatatgccc attctggatc taaatgagat tttttatgca
     2761 ctctgtaata gttatattgc gaaaaccaaa cgccagcagt aaagccagga gctatccaaa
     2821 ataatggaga aacagcaact gcaccaaata ccaaggcagc cacttctttg ctttgtggat
     2881 cccatgcgtc taaaatatta tgttcataat ccgaatctct aaattcttct tttaaaactg
     2941 tcttgtggtg cactgcccaa tgaaatcgcc agaaactatc ctttttcttg ccttgtccgt
     3001 gcaatacgtg tttgtggaaa taccattcaa aggcatttga cacaattaat ccattaataa
     3061 aaccgagcat acatcgaatt tttgttgatt aaatataaag ataattttca aataaatatc
     3121 catgtttgtc aaagtgcaaa acaatagttc gatttccttg tttatttttg ctaattatat
     3181 atgcaagtac aaacaattag attagcaaat tccatttcaa tccaaatatt tgaacatatt
     3241 tctgccattg atgaacaagt ctggaacagc attacaacaa atattttttt ctatcaaaca
     3301 catcagtttt tatctgtgat tgaagaaatt catactacta tccaatttag gtatgtattg
     3361 gtgtataaag aagataaaat cgttgcaggt ctatatacac aattattaca tttttcgttt
     3421 cgcaatctgg tcaattataa tgcaaatgct tcgactggca taaaaagtaa agtgaagcaa
     3481 tatattgcta aaaaagacac aaaattactc aacttaggta atgtgttttt tactggagat
     3541 aaaggcatta tttgtcagca agatgataat ataatttcgc tattaccaga tatttttaca
     3601 aaattgtatg cctcatttac aaagcaaaaa ccaagagctt atttggtggc gaatatctat
     3661 ttgcaagatg agtcaaaatg tgcttctttt tcaagccatg cttttcatcc ttttattact
     3721 gaaccagata tgttgatgcc aattgctgct gcatggacat cgtttgatga ttatttaagt
     3781 gcgatttctt caaaatatcg cgtacgtgat aaaaaaatat tagccttgtc ttcctcgatt
     3841 aaaactgtca attttacatt agaagaaatc aaaaataatc gagttcattt ttctcattta
     3901 tatgcgaatg tgattaatca tgttgccttt aatatggcta cattaagtgt tgattttttt
     3961 gaaagaatga aactgttgta tgtcgataaa tttaatatta ttggatatta caaagaagaa
     4021 gccttagttg gatttgcatg tttgtttaat gtagatgaaa acatgctaca tgtacattat
     4081 attggacttg attataccat taataaagaa caaaaactgt acaatcgtat gttgttagat
     4141 tttgtgaaat ttgctattga gaataagaaa aacaaaatcc attttggtcg aactgcaacg
     4201 gaaataaaat ctactattgg tgcgcaggca tttccattga atgcgtattt aaaaatgcac
     4261 aatccaatgc taaatatatc attaccctat tttttaagta gaataaaacc agctgaatat
     4321 acattgagaa atccgtttag agaactttct taacgattaa tatattcttc aaatgttttt
     4381 cgttgcattg tttcattttc ttgtagctta tacaaatcat ctttgtgcca gtttctaaaa
     4441 ataaatgcca atacaaaacc actgaaactg cctgctaaat gagattccca tgaaattcct
     4501 gcttgctcag gaataattcc ccaaaccatt ccaccataaa ataaagccac accactggcg
     4561 atggctatcg acttgatgtc catacgaaaa aaaccattga ataataaaaa agaagccatt
     4621 ccataaatga taccgcttgc accaatatga taggaatttc cacgtgcaaa gcaccacacc
     4681 aacaatccag ttatcaaata attacaaatg aaaatcaaaa tgcctatttt tttattcata
     4741 tacagcaata tgctacctaa gattaagatt ggcaaggtat tagaaataag atgctcccaa
     4801 ttgccatgta ttaatggcgc agtgaaaatg ccaatcagtg tatgactatc tcgtgggtga
     4861 atacctaaat agctgaaatt aacattcgta aagaaatgga taataaacaa ggtccacatc
     4921 aaacaagtaa atagtatagg tatcaagata gcctttctca tgattctcaa taggtaaggt
     4981 acaaagtacg gaattaattt tgttacaaat ttttattcca atgcatgcat attaatctta
     5041 acaaaacttc aattttatta acatgtagca taaatacgct gtaacattgt tattttagca
     5101 cacaaaattt tcaaaatcaa tgataatagg cattcccaaa gagaccaaat ttaaagaaaa
     5161 cagagttgcc attactccat taattgcaaa agatttaatc aaacaaggat ttacagttca
     5221 tgtagaaagt ggtgcaggtt taaattcatt cttctctgat gacaactaca aagaagcagg
     5281 cgcaacgcta tcagataaaa acacgacata tcaagcagat atagttttaa aagtaaatgc
     5341 accattacca gaagaaatag cattaatgaa aaaagatgca gttttaattt catttatgtg
     5401 ggcagcaacc aatccagaat tggtgagtgc atgtgaaaaa gcaggcattt cagcattttc
     5461 tatggacgca gttccgcgta tttcacgcgc tcaaaaaatg gacgcattat catcgcaagc
     5521 caacttggct ggatacaaag cggtaatcat gtgtgcagat acattaggta aaatttttcc
     5581 aatgatgact actgctgctg gtacaataaa accagctaaa gtggtgattt ttggtgctgg
     5641 tgttgctggc ttacaagcta ttgctaccgc aaaacgttta ggagccattg tagaagtatc
     5701 ggatattcga ccagaaacta aagagcaagt acaatcatta ggtggtaagt tcatcgaagt
     5761 aaaaggtgac gataccatca aaatggaagg tggttatgta aaaggagttt ctgatgaatt
     5821 tttaaaaaaa caacaagagc ttgttggtaa gcacgtagca gaagctgata tcgtcattac
     5881 aacagcatta atacctggta gaaaagcgcc agtattggtg acagaagaaa tggtaaaatc
     5941 catgaaattt ggttctgtta ttttagatat ggcagtcgaa caaggtggaa atgtggtagg
     6001 aagcaagtta aatgaaaatg taaatattaa tggtgttacc attattggag aaggcaacat
     6061 accttcttta ttgcctatga acgccagcga tttgtacgca aaaaatattc aaactttatt
     6121 atatcattta gctacaaaag atggatttaa atgggaaatg gaagaggaaa ttacgaaggg
     6181 aagtttaatt gttcaccaag gcaaaaatgt aaaagcttaa tcttttatag tttttttata
     6241 cgcaaataca aactaacaaa tatggaaata cttaattttt ttgtagaaaa tcgtgaaatg
     6301 atatatttca ttgtactatc tatttttgta ggcattgaag taataggtgg tgttcctacg
     6361 gtgttgcaca cgccattaat gtcaggtgca aatgccatac atggcgtagt aattgttggt
     6421 gcaatcattg tcatgttaga tacagaaccg aacaatatac ttggtttaat acttgggttt
     6481 ttagcagtaa ttcttggaac actcaatgtt gtgggtggat ttgtagttac tgaccgtatg
     6541 ctggaaatgt ttaagaaaaa ataatttagg cattaggcat tagacaatag acattaggca
     6601 atagacatta gttaaaagaa tggacaaaac aacaaaaata acatcttaca aagatttaat
     6661 tgtttggcag aagtcaatag attttgtttc ttatatttat ggcatcactt ctcaatttcc
     6721 atcagcagaa aaatttaatt taactagtca aattaataga gcagttatat ctattccagt
     6781 aaatattgcg gaaggttttg gtagagaaag ctcaaaaaat tacattcagt ttttaaaaat
     6841 ttcaagaggt tccttgtatg aggtagatac tttatttatt atcagtaaaa atctagaata
     6901 tattacaact gaagtatatt ctcaaactac tctatttatc aatgaaatat ctaaactttt
     6961 aaacggatta atcaaaagta ttcagaataa aataattaat taacgaagat acttatgcct
     7021 tgtgccaagt gtctaaatac taaataaaat aatgaaagga atattagaaa tatcttattt
     7081 aataggttcg ataaccttta tagttggatt aaaaatgatg ggaaatgcca aaactgctcg
     7141 taaaggaaat ttgattggtg catttggtat gactgttgcc attttaggta ccattttttt
     7201 gtatgaaaaa gaaggacaaa cttcaagaga gtttttcaaa attgctttaa tttttggagc
     7261 aattgctatt ggtactgcca ttggttggat tacagctaaa aaagtagaaa tgacaaagat
     7321 gccagaattg gtatcgatgt tcaatggtat gggtggtgcg tgcgcggcat taataggact
     7381 tacagaattt ccgcatgttg ctcatcaaat tcaagaaaca ggcacaggat atagttctat
     7441 ggcgtttatt attgctggtt tagttatagg tactatctca ttttctggtt ctattatcgc
     7501 ttatttaaaa ttggctggta aaatgaataa gccaatccgc ttgccacaat ataatatttt
     7561 gaataatgca ttattacttg tagtaatcgt attttcagcg tatgtagtat tgcaaagtac
     7621 aagtttgcca ccagcaattg tttgggcatt gtttgtcgtt gcgattgtgt atggcatttt
     7681 atttactatt ccaattggcg gcgctgatat gcctgttgtt atttcattat tgaactcttt
     7741 tactggttta gcggcagcat ttggaggctt cttgtatgac aacaaaccga tgctaacagg
     7801 tggtatttta gtaggttcgg caggtacttt attaacgctg gctatgtgta aagcgatgaa
     7861 tcgacctttg tcaaacgtaa tatttggtgc atttggcggt ggcagcggcg ctgctggtgc
     7921 tgatgctggc gaaaaaggta ccgttaaaga tgtacaagtg tcagatttgg cagttcaatt
     7981 aaactatgca agcaaagtag ttatcgttcc aggatatggt ttagcggtgg ctcaagcaca
     8041 acacgttata catgaactag aaaaaatatt ggaagaacga ggcgttgaag ttaaatatgc
     8101 tattcatcca gtagctggtc gtatgcctgg acacatgaat gtgttgttag cagaatcaaa
     8161 tgtggaatat gataaattag tggaaatgga agatatcaat ccacaatttg cacaaacaga
     8221 tgtggtttta gtagtaggag ctaacgatgt tgtcaatcca gcagcaaaag tagatccaag
     8281 ttcgccaatt tatggcatgc caattttaga agtagaaaat gctaagaata tagttatcaa
     8341 caaacgttca atgaatgctg gatatgctgg tatcgacaac ttattattct ataatccaaa
     8401 aacatctatg ttctttggag atgcgaaaaa agcattaaca gaattagtcg cagaaatcaa
     8461 acaattataa atagttttta atgctaattt taggtataaa gctggctcac gaagtgagcc
     8521 agcttttatt ttttagcaaa ggcaataata tgtccatcac agtcagcaaa ataacaagca
     8581 tagtcgcccc aatcgcgcag tgcaatatca ctgattaagc tagcgccaca gtcgattgcg
     8641 cgtttatatg cttgatgaat atcatctaca taaacatata attcacaacg tggaatccct
     8701 gagccattat ctggatgtgg tgttttgttt tgtaatattt ttgcaatact attagatggc
     8761 atcaatccaa gcctacagtt ttctacaatt tgaaaagatg tcatgccagg tacaaataga
     8821 acaggctttt tatctagcaa tttttcataa aattttgtgc ttgcattttg atcgcttaca
     8881 tataagataa tttctacgct attcataatc agtcttataa aattttcaat ctttttttcg
     8941 tgtgaacaat cctagcatcg atttcccttt gaaaagcaaa agctgtttat ttttaattct
     9001 atagtttgtt gcttgttgta gcatttgaat aaagattggt tcaatcgcat gcttgccaca
     9061 tttcattttt gtagtactaa tgttttcaaa taaaatgcta ctagagtcca tcgtaaaact
     9121 tgcagaaagc tcgttacagt cgctgtttcc gctaatggta ttgtttttaa tatcgaatcg
     9181 aatataagcc ttttgaagag aaaaggtttt tgtgcttcca tctttcatgc gaagtttata
     9241 caataaccat atactactgt cttctacagt atatattttt tttacgttcg tttcaagatt
     9301 tgaattttgt gtttttatct ttgattttat ctcacttaag ctatattgat atgtcgtaat
     9361 gttggtactc gtatctgtgt ttttacttac tgtagttaat agacaatatg tataacccgt
     9421 ttcataggta aaaccagcta tattttcata aaaaatggaa aaagtatccg taggtgtttc
     9481 ccttgtcatt aaatatttat tagttccaat tgtatggtat tggtcagcta cgtataatac
     9541 ttttagataa tcactcgaaa atgcttgagt acaaatgaac agacttataa ttaaaacaat
     9601 ttttttcata tttatactac taaattacct atttttgaga cataattcat gcttatgaaa
     9661 aagaaaatcc tacttgttct tgcgatagtt ttaattgtaa tacaatttat tcatccaaca
     9721 aaaaatcaac aagccaacat cttagattca gatataacaa aagttcgatc tgtgcctgaa
     9781 gatgttcagc aattgctaaa aacagcatgt tatgattgcc atagtaataa tactgtttat
     9841 ccgtggtata acaatattca gcctgttgct tggtggttaa ataagcatgt gaataatggt
     9901 aaaaaacaat tgaatttttc tgagtttggt gcttatgacg agaaaaaagc aaagcataaa
     9961 ttgcacgagc ttgttgaaac tttagaaaaa aatgagatgc cactttttag ttatacgatt
    10021 atacataaag acgcaaaatt aaatgatgct caaaagaaaa tcattattga ttgggcgaag
    10081 gctaatcaat aatttaataa gatagcttta tttattacta ttatgaagcg cttattgtca
    10141 aaaatctcgc ataactaaat agtcaattaa gctatttagt acagcttttt tttcgctatt
    10201 caaattaatt ttttccaaag cgtcaattga tttttggata tacatatcgc gtttttcaat
    10261 gccaaatgct tttgtattgt atgtattaaa aatagctaat atatcgttta ctttttcatc
    10321 attattaaaa acagattgtt ttaaaatctc attaagccgt gtgtcgttat tggcattact
    10381 tttttcaatg gcatgtatta acaacaaagt ttttttattt tgtataatat caccaccagg
    10441 ttgtttgcca accaattttt catcgccata gcaatctaat atgtcatctt gaatttgaaa
    10501 tgcaatgcct aaattttcag catattgata tagcaattcg gtgtcttctt ttttagcacc
    10561 gcctaaaata gcgccaattt tcatgctggc ggccaataaa acagatgttt tcaaacgaat
    10621 catttctata tattcatcgt gtgttacaac ttctttagtt tcaaaatcaa tgtcgtattg
    10681 ctgtccttcg catacttcaa tggcggtttt tgtataaaca tcaaacacgg ttttgaaata
    10741 tttctcattt acttttgcca aatactcaca tgcttttatc atcaagacat caccacttaa
    10801 tatagctgta ttcaaattat atttttcatg aatagtggta aatcctcgac gcaatggtgc
    10861 tttatccatt acgtcatcgt gtattaatgt aaaattatgg aaatattcaa tagctaaaga
    10921 tgccgccaac gattgttcaa tttctccatc aaaaatatca gtagcaagca acaataaaat
    10981 aggacgaagt cttttgcctc caatttgtaa aaaataatga ttaggctcgt acagattttt
    11041 aggttctatt attttgttct ctgtttgcag gtacgctaaa aaaatatttc ttaattcttc
    11101 aatagaatgc atctgattaa caatttctaa caaaaatata tcttttatag aactaaattt
    11161 gatttaccgc attattatta tgtatgaatt ctgaattaca atttgaatgg acaaacgttt
    11221 tcgacaaaac tatagccata tgtgaagatg accttaatta caatacttat tcagctcgcg
    11281 aaaaaatgct tgcttttgtt tttacattct tgcaatcatt g
//
LOCUS       DAOQQV010000081        11218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2063_length_11218_cov_8.681358, whole genome shotgun sequence.
ACCESSION   DAOQQV010000081 DAOQQV010000000
VERSION     DAOQQV010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2063_length_11218_cov_8.681358"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLL59_08815"
     CDS             <1..257
                     /locus_tag="PLL59_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HMY23950.1"
                     /translation="CNRNYLTPQQEKQMSTQPDMILQFAHFLANEYETKKGFKNAEVY
                     ADSYAALNGHPSQAFIDNTIDLAKQPINFAPKKWILPFKK"
     gene            254..2437
                     /locus_tag="PLL59_08820"
     CDS             254..2437
                     /locus_tag="PLL59_08820"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMY23951.1"
                     /translation="MKQVQFYISIIIFLSLQNSYAQTNDADLSYFDTIQTQIDEITIQ
                     AEKMKEIRLKSVEGTVIFEGKKTEIVDLSNILANTATNNGRQIFNRIAGLNVWENDAA
                     GMQIGIGARGLNPNRSAEFNIRQNGYDITADAIGYPDAYFTPPTEALDRIEIVRGAAS
                     LQFGTQFGGLINFKTHQAINKKFQITSQLTGGSFATVSAFNAIEGSYKKFSYYAYYQY
                     KHTKGWRPNSKLNAHTAFANISYQVSKKVNISFEFTHQNYIMQQPGGLTDNQFRINAK
                     QSTRTRNWFSINWNVPALKVEYQPKENIRWNSVVFGLIGSRNALGFLSYANRNDDFKE
                     RDLIKDKYKNIGLESRILYQYKLGKNQSSLSVGVRYFRGFTMKQQGLGNNEDDADFNY
                     LHPNDLENSDYHFIGNNLAISAENIFKITPKLSITPGVRMEILFTDVNGYYKTDSILY
                     ADDRNYKRIFPLLGIGISYKAHHLAEVYANISQNYRGINYTDLRITNPNVRVDPNIKD
                     EKGFNGDIGIRGKSGDKFNYDINFFSLYYNKKVGSIQQVDSQYNIYRYKTNISNSRSL
                     GVESFFEVDILKCIREYSDFGLSIFSNFTFTDAVYVKSQNKQLVNKKVEFAPQYIFRG
                     GITFKYKSFSLAWQISTVSKQFTDATNAKYASTAVIGEIPRYYVMDISTSYSYKWFKF
                     SCSINNISDNRYFTRRAESYPGPGIIPSDGIGAFGTIAFKWNTKN"
     gene            2511..3596
                     /locus_tag="PLL59_08825"
     CDS             2511..3596
                     /locus_tag="PLL59_08825"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HMY23952.1"
                     /translation="MQKFSTYFILLLAILTTVSCKKDNKGVYDAEINNITDNIIIATY
                     SDLDAQAAVLLDAVNALKNGGATSVELATARTAWQATRAPWEASEGFLFGPVSDNEID
                     PNIDTWPLDSIGVDNILAGTDIINKTYIDNLTGDAGTGLKGFHAIEYFLWGTDGNKVV
                     ADFTARELDYLIAAVESLKGKTAELKNAWISSGGNFAKQLKDKSSRYPSEVDVLIELA
                     EGIRGIAGEVFDTKIQNTLDGLDADGVAGGIREEESRFSNNTKSDFTNNIQSIANVYL
                     GLYATTGNGKGLSDVVASKNTTLDAKIKLQISEAIAGIKSIGGSTSATYSAALEHDRL
                     SIEAAQTKVATLEETIRTELIPLISGL"
     gene            3669..5027
                     /locus_tag="PLL59_08830"
     CDS             3669..5027
                     /locus_tag="PLL59_08830"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HMY23953.1"
                     /translation="MKKLTYLLFFCVLIACEKENNMNNDATEEFPGGATTYLNIFSGA
                     FEQPSSNLSNAELAQHATGDVGFSANFITSPAINNPGLGPIFNHVSCVACHPKNGKSP
                     QPLSGADLKGLLFRVSIPRADESEGPMPVPGLGGQFQQKAIVGTQSEGQVSITFTETS
                     HTFADGTPYTLRKPNYTITSSTASIPANMMISPRIAQQVIGLGLIEAIKEQDILSQQD
                     INDSNSDGISGKANRVFNPATNQYELGRFGWKANNASLLEQVAGALHQDIGITSFIFP
                     TEASYGQSQYDGLADDPELDSQAVIDLTFYTQSLAVPKRRFFNDPAANKGKEIFFNIG
                     CNKCHTQKYTTGTTGFGFVSNQTIYPYTDLLLHDMGNELADNRPDFLANGNEWRTPPL
                     WGIGLTELVNGHTSFLHDGRARSILEAIMWHGGEAEAQKVQVSKLSKTERENLIKFVS
                     SL"
     gene            5063..6199
                     /locus_tag="PLL59_08835"
     CDS             5063..6199
                     /locus_tag="PLL59_08835"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMY23954.1"
                     /translation="MKYKILLGFLYCFLFIACKREVKPFYYNSEEEFIANNEGLSDSA
                     RIGKLIFFDKNLSEPIGVSCASCHSPMTGFSDPRHLAFSVGSTNKEGGRNANAINYMG
                     FAPNRRAELVRGVWETVGGFFWDGKAEFLNQQALFPFTTAHEMNNISFSNVSSKLKRA
                     PYYNQLEKLFGKEVLSDSQMIVFYATMCLEAFELSHQVNQFTSKFDYYLAGKIKLSEQ
                     EMRGMEIFNDTTKSKCSLCHLSEPTPYASTKKIIFTDYSYDNLGLPKNPLQQHLPIDS
                     GLAKFEMNNPIEVGRFKTPTLRNIEKTAPYMHSGIFKDLEEVMEFYNERDINQKFAHP
                     EVASTINKDDLGDLKLTKQEINDIIAFLKTLTDGYRLPSTEKNN"
     gene            6162..6509
                     /locus_tag="PLL59_08840"
     CDS             6162..6509
                     /locus_tag="PLL59_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23955.1"
                     /translation="MDIDFHQQKKIINMKKLAIISILTLVVFACSKKTTVTKTSTTEK
                     TSSATVSHERFLAGKTVYEAKCGKCHKLFEPSHGNMESWKKWIGWMAPKAKLTDEETA
                     LVTDFVSVNALPN"
     gene            complement(6584..7606)
                     /locus_tag="PLL59_08845"
     CDS             complement(6584..7606)
                     /locus_tag="PLL59_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HMY23956.1"
                     /translation="MIIRSKAPLRIGLAGGGTDVSPYSDIYGGAILNATLSLYAYASI
                     QTRDDGKIVLNAIDRNEKIILDSEEELPIDGSLDLLKGVYNRIVKDFTKRPLSFEINT
                     FVDAPPGSGLGSSSTLVVAILGAFVEWLKLPLGEYDIAHLAYSIEREDLKMAGGKQDQ
                     YAATFGGVNYMEFYANDKVIVNPLRIKSKFLHELEHNLVLFYTQTSRLSSKIIQEQTD
                     NVHAKKSSSIEAMHQLKEQSLLMKEAVLKGEIDNIGKILHDGWMHKKQMADGISNASI
                     DAIYDAALQAGATGGKISGAGGGGFMIFYAPYIHKYSLIKRLSELGGSVMQYTLTTEG
                     LTTWTV"
     gene            complement(7637..8761)
                     /locus_tag="PLL59_08850"
     CDS             complement(7637..8761)
                     /locus_tag="PLL59_08850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY23957.1"
                     /translation="MKVVILGSAHPLRGGIAAYNERLARAFATQQDTVSIETFSLQYP
                     EFLFPGTTQYSSQPKPNDLTIHVSVNSINPFNWIKIGWKIRQQKPDILLLKFWLPFMA
                     PCLGTIARIVKSNKHTKVISILDNIIPHEKRIGDTVLTQYFVNSVDAFIAMSDSVYRD
                     LSTFDNRKPRLLHPHPLFDNYGDSVDKKNAIHALNLDASKKYILFFGLIRDYKGLDIL
                     LKAMATDALRHSDIHLIVAGEYYANKDEYEQLIQALDLQNKLVLHTHFIPDDEVYKYF
                     CAADMVVQPYKHATQSGVTQICYHFNKPMIVTNVGGLPEIVPNNKVGFVVEPDEHAVA
                     HAIITFYSENKEQDFIQHIVEEKKKYSWQAMVEKVKSFLQ"
     gene            complement(8790..9851)
                     /gene="leuB"
                     /locus_tag="PLL59_08855"
     CDS             complement(8790..9851)
                     /gene="leuB"
                     /locus_tag="PLL59_08855"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HMY23958.1"
                     /translation="MNKKIAVLPGDGIGPEIIEQAVKALQAIEKKFNHSFEYNYGLIG
                     AAAIDKLGNPYPDDTHTLCSNSDAILFGAIGDPKYDNDPSAKVRPEQGLLKMRSNLGL
                     YANIRPVKSYDALIEASPLKNDRIKNADFVVVRELISGIYFGKPRGRSEDNTIAYDTC
                     VYTREEILRITEKAFQFAKNRKKKVTLLDKANVLATSRLWRETVTEYAKQFPDIELEC
                     NFIDSAAMEVITKPTKFDVILTENLFGDIISDEASVITGSLGMLPSASLGNKVSLYEP
                     IHGSYPQAAGKNIANPMATILSAALLLEYSFGLLEESKTIVNAVERAMNEGIVTEDIN
                     NGKYSTSAVGDKIVEYILN"
     gene            9987..>11218
                     /locus_tag="PLL59_08860"
     CDS             9987..>11218
                     /locus_tag="PLL59_08860"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY23959.1"
                     /translation="MNNTVASKVKAKQLLLFDEQYVHAMRLFTQETLRSTKEKNHFES
                     ADNIYYIAECLLQLRRLDKVEKVLNKCADFIKLHNRNISIAFKNLQGKYLIEKGKYTA
                     CVDLLLPIKKGNTNDSLWCELQITLADAYQRLQIEDKCLMHVQATLQHTKDNFQKART
                     LNLLGAYYWLLSELDSSKISFEKSYALCLASVGKQHSKSAQLLYNLGLLASRNADYIA
                     AENYYNQSLQIFIDKLGHSHPKTADAYGALGSLFLIEDNPEKARYYSQKEVSIILKNY
                     GNTHPTLAYGYLNLGKIYYLLNDYSKAEKYLNEAISIVQTQYGKQHNLYKQCVVKLAE
                     LWTTQKKYEQAKHLLSEAKLLPKDEYTADIFLQIGSTYLAEKSYSLAIEHLKQANTIF
                     EEYYGADNIYATDVQLELS"
BASE COUNT         3777 a         1998 c         1948 g         3495 t
ORIGIN      
        1 tatgcaacag aaattatttg acaccacaac aagaaaaaca aatgagcaca cagcctgata
       61 tgatattaca gttcgctcat tttttggcaa atgaatacga aacaaaaaaa ggatttaaaa
      121 atgcggaagt gtatgcagat agttatgcgg cattaaatgg ccatccaagt caagctttta
      181 tcgacaacac tattgattta gccaaacaac ctattaactt tgcacccaaa aaatggattt
      241 tgccttttaa aaaatgaaac aagttcaatt ctatatttcc attataattt ttttaagcct
      301 gcaaaattcg tatgcacaaa cgaacgatgc tgacttatcc tattttgata ccattcaaac
      361 gcaaattgac gaaatcacca tacaggcgga aaagatgaag gaaatacgtt taaaaagcgt
      421 tgaaggaact gttatttttg aagggaaaaa gactgaaatt gttgatcttt ctaatatatt
      481 ggcaaataca gccacgaata acggaagaca aatatttaat cgaattgctg gtttaaatgt
      541 ttgggaaaat gatgccgcag gcatgcaaat tggaattggc gctcgcggat taaatccaaa
      601 tcgttcggca gaatttaata tcagacaaaa tggatacgat atcactgctg atgccattgg
      661 atatcctgac gcttatttta caccaccaac ggaagcatta gacagaatag agattgtgcg
      721 tggcgctgct tcattgcaat ttggcacgca gttcggtggt ttaattaatt ttaaaacgca
      781 tcaagctatc aataaaaaat tccaaattac atcgcaactc acaggcggtt cttttgcaac
      841 tgtaagtgca tttaatgcta tcgaaggcag ctataaaaaa ttctcttatt acgcttatta
      901 tcaatacaaa cacacaaaag gttggcgacc aaactcaaaa ctcaatgcac atactgcttt
      961 tgcaaatata tcttaccaag tttctaaaaa agtcaatatc tcatttgagt ttacacatca
     1021 gaattatatc atgcaacaac ctggtggctt gacggacaat caatttagaa taaatgcaaa
     1081 acaatcgact cgaactcgaa attggttttc aattaactgg aatgtacctg cgctaaaagt
     1141 agaatatcaa ccaaaagaaa atatcagatg gaattctgta gtttttggac taatcggcag
     1201 tcgcaatgcc ttaggttttt taagttatgc caatagaaat gatgatttta aagaacgtga
     1261 tttaattaaa gataagtata aaaatattgg attggaatct cgtattttat atcaatataa
     1321 attgggcaaa aatcaatctt cattgagcgt cggcgtccga tatttcagag gatttaccat
     1381 gaaacaacaa ggattgggca acaatgaaga tgatgcagac tttaactact tgcatccaaa
     1441 tgatttagaa aattcagact atcattttat aggtaataat cttgcaatat cagcagagaa
     1501 tatttttaag ataacaccca aactcagcat cacaccaggc gtcagaatgg aaatattatt
     1561 tactgacgta aatggttact ataaaacaga ttctatactg tatgctgatg acagaaatta
     1621 caaacgcata tttccattat taggtattgg catcagctat aaggcgcatc atttagcaga
     1681 agtatatgcc aatatttctc aaaattatag aggaataaac tatacagatt tacgcatcac
     1741 aaatccgaat gtacgtgtag atccaaatat taaagacgaa aaaggattta atggcgatat
     1801 cggtattcgc ggaaaaagtg gcgataaatt taattatgac atcaactttt tttctttgta
     1861 ttacaacaaa aaagtaggct ctattcaaca agtcgattcg caatataata tctatcgcta
     1921 caaaacaaat atatctaact ctagaagcct aggtgtcgaa tcattttttg aagtagatat
     1981 cctaaaatgt attcgcgaat actcagattt tggactctca attttctcta attttacatt
     2041 tacagatgct gtttatgtca aatctcaaaa caagcaatta gtcaacaaaa aagtagaatt
     2101 tgcaccacaa tatatttttc gtggtggcat cacttttaaa tacaaatctt tttcactcgc
     2161 ttggcaaatt tctactgtgt ccaaacaatt tacagatgcc acaaatgcca agtatgcatc
     2221 tactgctgtt attggagaaa ttccaagata ttacgttatg gatattagca cttcctattc
     2281 ctataagtgg tttaagttta gctgctctat taataatatt tctgataatc gatattttac
     2341 cagaagagca gaaagttatc ctggacctgg catcataccc agtgatggca tcggcgcatt
     2401 tggcacaatc gccttcaaat ggaatactaa aaattaggta ttatttttat ttatcttagg
     2461 tatttgaatt tatttagact aattctattt ttgttcctca ataaaaagtt atgcaaaaat
     2521 tttctacgta ttttattctt ttattagcta tacttacaac agttagctgt aagaaagaca
     2581 acaagggtgt ttatgatgct gaaatcaata atatcaccga taacattatc attgctactt
     2641 atagcgattt agatgcacaa gcagccgttt tgttagatgc cgtaaatgca ttaaaaaatg
     2701 gtggcgccac ttcagtagaa ctagcaacag caagaactgc ttggcaagca acacgcgctc
     2761 cttgggaagc tagtgaagga tttttattcg gaccagtttc tgataatgaa atcgatccaa
     2821 acatcgatac ttggccacta gacagcattg gcgttgataa tatattagca ggcactgata
     2881 tcatcaacaa aacatacatt gacaatctga ccggcgatgc tggcacagga ctaaaaggat
     2941 ttcatgcaat cgaatatttc ctttggggca ctgatggcaa caaagtagtt gctgatttta
     3001 ctgcacgcga gttagattat ttaattgcag cggttgaaag tctaaaaggt aaaacagcag
     3061 aactaaaaaa tgcttggatt tcatctggtg gaaacttcgc caaacaacta aaagacaaaa
     3121 gctcaagata tccaagtgaa gtagatgttt taatagagct agcagaagga atacgcggta
     3181 ttgctggtga agtatttgat acaaaaattc agaatacctt agatggctta gatgctgatg
     3241 gcgttgctgg tggcattcgc gaagaagaaa gtagatttag caacaacaca aaatctgatt
     3301 ttactaataa catacaaagt attgctaatg tttatttagg tctatatgct actactggaa
     3361 atggtaaagg cttatcagac gtagtcgctt ctaaaaacac tactttagat gcaaaaataa
     3421 aattacaaat tagcgaagct attgctggta ttaaatcaat tgggggttct acaagcgcta
     3481 cctattcagc agctttagaa cacgatagat tatctatcga agccgcacaa acaaaagtgg
     3541 caactttaga agaaaccatt agaacagaat taattccatt aattagtggt ttatgattaa
     3601 taaactaaat acaatatatt cttcattaaa atgtctcgac ttttcgagac atttattttt
     3661 taatttttat gaaaaaactc acttatctcc tgtttttctg cgtgctcatt gcatgtgaaa
     3721 aagaaaacaa catgaacaat gatgccactg aagaatttcc aggtggcgct accacttatc
     3781 tcaatatttt tagtggtgca ttcgaacagc cttcctctaa tttatcaaat gcggagctgg
     3841 cacaacatgc gacaggagat gtcggatttt cagcaaattt tattaccagt cctgccataa
     3901 ataatcctgg acttggacct attttcaacc atgtttcttg tgtggcttgt catcccaaaa
     3961 atggaaaatc accgcaacca ttatctggtg ctgacttaaa aggtttatta ttcagagtaa
     4021 gcattccacg tgcagatgaa agcgaaggcc ctatgccagt tccaggttta ggtggtcaat
     4081 ttcagcaaaa agcaattgtt ggcacacaat ctgaaggaca agtaagtatt acgtttacag
     4141 aaacatcaca cacgtttgct gatggcacac cttatacact tcgcaaacca aattatacta
     4201 tcacttcatc aacggcaagt attccagcga atatgatgat atctccaaga attgcacagc
     4261 aagtcattgg attaggttta attgaagcca ttaaagaaca agatatttta tcacaacaag
     4321 atatcaatga tagtaatagc gacggtattt ctggcaaagc taatcgagta tttaatccgg
     4381 ctactaatca atacgaatta ggtagatttg gctggaaagc caataatgct tcattgctag
     4441 aacaagttgc tggcgcattg catcaagata ttggtattac atcatttata tttccaacag
     4501 aagcatctta tggacaatca cagtatgatg gcttagctga tgatccggaa ttagatagcc
     4561 aagcagttat tgatttgact ttctatacac aatcattagc ggttcctaaa cgccgatttt
     4621 tcaatgaccc tgcggccaac aagggcaaag aaatattttt caatattggc tgtaacaaat
     4681 gccatacgca aaagtacacc acgggcacta ctggttttgg ttttgtaagc aatcaaacta
     4741 tttatcctta tactgattta ttgttgcacg atatgggcaa tgaattggct gataatcgac
     4801 ctgatttttt agccaatggt aatgaatgga gaacaccacc actttggggc attggcttaa
     4861 ccgaattagt gaatggtcat acaagttttt tacatgatgg aagagcaagg tctatattag
     4921 aagccattat gtggcacggc ggcgaagcag aagctcaaaa agtgcaagta tcaaaattat
     4981 caaaaacaga aagagaaaat ttaataaaat ttgttagctc attataatgc aagttttgat
     5041 ttgcttttat tacatttagc taatgaaata taaaatacta ctcggatttt tatattgctt
     5101 tctttttatt gcatgtaaaa gagaagtcaa gccgttttat tataattctg aagaagaatt
     5161 tatagcaaat aatgaaggat tatcagattc ggcacgcatt ggaaagctta ttttttttga
     5221 taagaattta tccgagccta ttggtgtaag ctgtgcttcc tgccattctc caatgacagg
     5281 attctcagac ccgaggcacc ttgcattttc agttggttcc accaataaag aaggcggtcg
     5341 caatgccaat gctatcaatt atatgggctt tgcaccaaat cgcagagcgg agttggtcag
     5401 aggagtatgg gaaactgttg gcggtttttt ttgggatggt aaagctgaat ttttaaacca
     5461 gcaagcttta tttcctttta caacagcaca cgagatgaat aatatcagct ttagcaatgt
     5521 ttcctcgaaa ttaaagcgtg caccttacta taatcaattg gaaaaattat ttggcaagga
     5581 agttttatcc gacagtcaaa tgattgtttt ttatgcaacc atgtgtttag aagcatttga
     5641 attatctcat caagtgaatc agttcaccag taagtttgat tattatttag caggaaaaat
     5701 aaaattgagt gaacaagaaa tgcgaggcat ggaaattttt aatgatacaa cgaaatcaaa
     5761 atgttcttta tgccatctaa gtgagccaac accgtatgct tctactaaga aaatcatctt
     5821 tacagattac tcttatgaca acttaggatt gccaaaaaat ccgctacaac aacatctgcc
     5881 aatagatagt ggtttggcaa aatttgaaat gaataatcct atagaagtag gcagatttaa
     5941 aacaccaaca ctcagaaata ttgagaaaac agcaccttac atgcattctg gtatttttaa
     6001 ggacttggaa gaagtaatgg aattttataa tgaacgcgat ataaatcaaa aatttgcaca
     6061 tccagaagtg gcctctacta ttaataaaga cgatttaggt gatttaaaat tgacaaaaca
     6121 agaaataaat gatataattg catttcttaa aacattaaca gatggatatc gacttccatc
     6181 aacagaaaaa aataattaat atgaagaaat tagccattat tagcatttta acacttgtcg
     6241 tatttgcctg ttcaaagaaa acgacagtaa caaaaacaag tactacagaa aaaacttcta
     6301 gcgcaacagt ttcacacgaa cgatttttag ctggtaaaac agtgtatgag gcgaaatgtg
     6361 gtaaatgcca taaacttttt gagccgtcac atggcaacat ggaaagctgg aaaaaatgga
     6421 ttggctggat ggcgccaaaa gctaagctta ctgatgaaga aacagcatta gtaacggatt
     6481 ttgtatctgt aaatgcatta ccaaactaag cttatcttac ctatacgcat aaaaaaagtt
     6541 gctcttcgag caactttttt cattttatta ttctgtaatt tcattaaaca gtccatgtcg
     6601 ttaaaccttc tgtggttaat gtatattgca tcaccgaacc accgagctca ctcaaccttt
     6661 taattaatga atatttgtga atgtatggcg cataaaaaat cataaagcca ccgccgccag
     6721 cgcctgatat tttgccacct gtggcgccag cttgcaaggc agcatcatat attgcatcga
     6781 tagaagcatt agaaatgcca tctgccattt gttttttatg catccaacca tcgtgtaata
     6841 tttttcctat gttgtcgatt tcccctttta aaactgcctc tttcatcaag aggctttgtt
     6901 cttttagctg atgcatcgct tctatagaag acgatttttt agcatgcaca ttgtcggttt
     6961 gttcttggat aattttagac gacaatctcg atgtttgtgt ataaaacaaa accaaattgt
     7021 gttctaactc atgcaagaat tttgatttta tgcgcaatgg attgacaatc actttatcat
     7081 tagcataaaa ctccatatag ttgacgccgc caaatgtagc agcatattga tcttgttttc
     7141 cgcctgccat ttttaagtct tcgcgctcaa tagaataagc taaatgtgca atatcatact
     7201 cgcctaatgg caattttaac cactcaacaa aagcacctaa aatggcgaca actaaagttg
     7261 aggacgagcc caatcctgag ccaggaggcg catctacaaa cgtattaatt tcgaaagaca
     7321 atggtcgctt ggtgaagtct tttacaattc gattatacac gccttttagc aaatctaagg
     7381 agccatcgat gggcaattct tcttcgctat ctaaaataat tttttcattt ctatcaatgg
     7441 cattcaggac aatttttcca tcgtcacggg tttgaataga agcataagca tataagctca
     7501 aagtagcgtt taaaatagcg ccaccataga tatcagagta tggtgaaaca tcagtgccac
     7561 cacctgctaa gcctatacgt aaaggtgctt ttgaacgaat aatcatgcag ctaatttaca
     7621 attttttgtt agaaccttac tgtagaaaac tcttcacttt ttctaccata gcttgccagc
     7681 tatatttttt cttctcttct actatatgct gtataaaatc ttgttcttta ttttctgaat
     7741 aaaaagtaat aatagcatga gcaacggcat gttcatcagg ctctactaca aaaccaactt
     7801 tattatttgg cacaatttca ggcaagccgc caacgttggt gactatcatc ggtttattga
     7861 aatgatagca aatttgtgtg acgccacttt gtgtcgcatg tttgtaaggt tgcactacca
     7921 tatctgctgc acagaagtat ttatacactt catcatcagg aataaaatgt gtgtgcaata
     7981 ccagcttgtt ctgtaaatcc aaagcttgaa ttaattgttc atattcatct ttgttagcat
     8041 aatattcgcc agctacaatc aagtgtatat cggagtgtcg caaagcatct gttgccattg
     8101 ctttcaacaa aatatctaat cctttgtaat cgcgaattaa gccaaaaaat aaaatgtatt
     8161 tttttgatgc atctaaattc aatgcgtgta tggcattttt tttatcaacg gaatcaccat
     8221 aattatcgaa caatggatgt ggatgtagca agcgtggttt tctgttgtca aacgtgctta
     8281 aatcacgata tacactatca ctcatagcga taaaggcatc gacactattt acaaaatatt
     8341 gagtcagcac agtatcgcca attctttttt cgtgtggaat aatattatct aaaatagaaa
     8401 ttactttcgt gtgtttattg cttttcacaa ttcgtgctat tgtgcctaaa cacggtgcca
     8461 taaagggcag ccaaaatttc aggagcaaaa tatctggttt ttgctgtctt attttccagc
     8521 caatttttat ccaattaaat ggattgatag aatttacgct tacatgaatc gttaaatcgt
     8581 ttggctttgg ctgtgatgaa tattgagtgg tgccaggaaa tagaaattct ggatattgta
     8641 atgaaaaagt ttcgatgctg acagtatctt gttgtgtggc gaatgctcta gcgagtcgct
     8701 cattgtaagc agcgatgcca cctcgcaaag gatgtgccga accaagaata accactttca
     8761 taagctaatt gtaattgcta aaactacatc taatttaaaa tatattctac aatcttatcg
     8821 cctactgctg atgtggaata ttttccattg ttaatgtctt ctgttacgat gccttcattc
     8881 attgcacgtt ctacggcatt gacaattgtt ttgctttctt ctaacaatcc aaagctatat
     8941 tctaacaaca aagctgctga taaaattgtc gccattggat tggcaatgtt tttgcctgct
     9001 gcttgtggat atgaaccgtg aattggctca tacaatgaga ctttattacc taatgatgct
     9061 gatggcaaca tgcccaaact gcctgtaatt acagatgctt cgtcgctgat gatatcacca
     9121 aataaatttt ctgtcaaaat cacatcaaac ttcgttggct tcgtaatcac ttccatggct
     9181 gcactatcaa taaaattgca ttctaattca atatctggaa attgttttgc atattccgta
     9241 accgtttcgc gccacagacg tgaggttgcc aacacattgg ctttgtctaa aagtgttact
     9301 ttcttttttc tattttttgc aaattgaaat gctttttctg tgatacgcaa aatctcttct
     9361 cttgtgtaaa cacaagtatc gtaagcaatt gtattatctt cgcttctgcc gcgtggtttt
     9421 ccaaaataaa taccagaaat caattcgcgc acgacgacaa aatcagcatt tttaattcta
     9481 tcattcttta atggcgatgc ttcgattaag gcatcataac tttttacagg tcgaatattt
     9541 gcatacagtc ctaaattgct acgcattttt agtaagcctt gttctggacg cacctttgcg
     9601 cttggatcat tatcatattt cggatcgcca attgcaccaa acaatatagc atcgctattg
     9661 ctacacaagg tatgtgtgtc gtcaggatat ggattgccaa gtttatcaat ggcagcagca
     9721 ccaattaaac cataattata ttcgaaacta tgattgaatt ttttctcaat ggcttgtaaa
     9781 gcttttacag cttgttcgat aatttctgga ccgatgccat cacctggcaa tactgctatt
     9841 ttcttattca tgtatcaaaa ataaacatac aaaatgtata aaatatagga ttggtgttat
     9901 taaatttata tggatagaca cacactttga gattatttgt attcatactg tttattatcg
     9961 ttgctccgac aatacttagc gccaccgtga acaatacagt agcaagtaaa gtaaaagcaa
    10021 aacagttatt attgttcgat gaacaatatg tacatgcaat gcgcctattt acgcaggaaa
    10081 ctttacgttc tacaaaagaa aaaaatcatt ttgaaagcgc tgacaatatc tattacattg
    10141 cagaatgttt gttacagcta aggcgattag acaaggtaga aaaggtgctc aataaatgtg
    10201 ctgattttat caagttgcac aatagaaata tatctattgc tttcaaaaat ttgcaaggaa
    10261 aatatttaat tgagaaagga aaatacactg catgtgttga tttattattg ccaataaaaa
    10321 aaggtaatac aaatgattcg ttgtggtgtg aactacaaat tacattggct gatgcttatc
    10381 aacgcttaca aattgaagac aaatgtctta tgcatgtgca agccacattg caacacacaa
    10441 aagataattt tcagaaagca cgtactttaa atctattagg tgcttattat tggttactat
    10501 ctgagttaga ctcctcaaaa ataagtttcg aaaaatcgta tgctttatgt ttagcaagtg
    10561 taggcaagca acatagtaaa tcagcacaat tgctatataa tcttggattg cttgcttcac
    10621 gaaatgctga ttatattgct gctgaaaatt attacaatca gtcattacaa attttcatcg
    10681 acaagctcgg tcattcacat ccaaaaacag cagatgctta tggtgcctta ggaagcttat
    10741 ttttaataga agacaatcct gaaaaagcgc gttattattc tcaaaaggaa gtgagcataa
    10801 tattgaaaaa ttatggcaac acgcatccta ctttagccta tggatatttg aatttaggaa
    10861 aaatatatta cctacttaat gactattcga aagctgaaaa atatttgaat gaggctattt
    10921 caatagtgca aacgcaatat ggcaaacaac ataatttata caaacaatgt gtagtgaagc
    10981 ttgctgaatt atggactaca caaaagaaat atgagcaagc aaaacacttg ttaagcgaag
    11041 cgaaattact acctaaagac gaatacactg ctgatatctt tttgcaaatt ggcagcactt
    11101 atttagctga aaaatcctat tcattagcca tagaacatct aaaacaagcg aataccattt
    11161 ttgaagaata ctatggtgct gataatattt atgccaccga tgtacaatta gaattatc
//
LOCUS       DAOQQV010000082        10933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2162_length_10933_cov_9.589079, whole genome shotgun sequence.
ACCESSION   DAOQQV010000082 DAOQQV010000000
VERSION     DAOQQV010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2162_length_10933_cov_9.589079"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLL59_08865"
     CDS             complement(<1..357)
                     /locus_tag="PLL59_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HMY23960.1"
                     /translation="MQDKKWISRTTALIGDDNLNKLINSHVLVVGLGGIGSFAAEFIA
                     RAGVGTMTIVDGDVVDPSNRNRQLPALYSTHGVSKADWMAERIKDINPEIKLNVIKEF
                     LSPEKAYEIVSLYEYDY"
     gene            453..1052
                     /locus_tag="PLL59_08870"
     CDS             453..1052
                     /locus_tag="PLL59_08870"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMY23961.1"
                     /translation="MLQHFPSDKIICGVDEAGRGCLAGPVVAAAVILPKNFTHEFLND
                     SKQLSEIQRNELRPIIEKNAIAFGVGIIDNNKIDEVNILQATYLAMHEAIAQIKQKID
                     LLIIDGNRFKPYKNIAHQTIIKGDGKYIEIAAASILAKTYRDEIMEKLHIEFPEYHWH
                     KNKGYPTIAHRNAIKNLSACVYHRQTFRLLKEEQLNLFE"
     gene            complement(1116..2357)
                     /locus_tag="PLL59_08875"
     CDS             complement(1116..2357)
                     /locus_tag="PLL59_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HMY23962.1"
                     /translation="MKISKHSTFALLILGMLVASCTKNTPVLPVADFSANSLAIRTDS
                     AVNFTDESTGNPTSWSWSFQGGTPSTSTLQHPTNIQYHAVGSYNVTLTVTNADGSNTK
                     TKSGYIVVTELPVTLPTVSTSTVSSIAKFSAVSGGNISNSGNSIITARGICWSTNPNP
                     TILNNKTTDSNGIGNFISNLSNLKSNTKYYVKAYATNSVGTAYGNEISFTTEAFPNCG
                     TVTDIDGNVYHTVTIGTQCWMVENLNTTRYNDGSPIKTGLSELEYTQTLEGVYSHCNN
                     NPGNDAVYGKLYNWYAVYTGKLAPTGWHVPSIAEWTVLFNYLGGTNYAGCQMKATDVF
                     WKSPNVGASNSSGFSGFPAGKHDGFWGGYKTFNEIGGFWSNANHTATDAWWCVLNYDR
                     CDGYLTTTYMKDGYSVRCIKD"
     gene            complement(2379..3767)
                     /locus_tag="PLL59_08880"
     CDS             complement(2379..3767)
                     /locus_tag="PLL59_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23963.1"
                     /translation="MKKTLNFKMQAALLFTLICLLNACTPDSSTPDGIATVSTATPIK
                     VDDTTYTLGGNVTNQGSSAVTENGVAIALTANPVIDDPDGATIAIADGTGSFSTEVAP
                     FAGGYTYYVRAYAKNSSGVAYGNQVTITTDAVAACNEIEISSNITAVTTWTSGNVYII
                     NNWINIKAPLTIQPGVIVKFKNANCGLEVYAKVTANATAANPIIFTSYKDDSYCGDNN
                     GDGAATTANKGDWAYINMRGDQHGSVFRYCKFLYGGGQGGSVVRANVGTGNIHDFTFD
                     YCTFAHTYGTNSPNTAAFNGGDTKDPSVSVITNCAFYDNAIPIYINSYYSIDQNNIYH
                     NPNNPSETNKYNGIYVYSFGLSGKTVPYTETEVPYVLLQGLSAGGSGNLFSVGANVII
                     KIPAGSGEEISGYTANFAIHPSAIFTSLRDDTRGGDTNGDGNASSPSANDWNGVNING
                     VWYHNNVFYAKN"
     gene            4016..5977
                     /locus_tag="PLL59_08885"
     CDS             4016..5977
                     /locus_tag="PLL59_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMY23964.1"
                     /translation="MKKTALLILSSFVFITTYAEISYKDSLRNAISKAPTDSLRIESM
                     FQYMKATLNDNTSDFEPYIKEMIKLSKKINYKWGLSTAYVLGISYYRHQADYETALYY
                     ASLIEELCKNDTSKDIRINLGHMHQNRGNLFSTIGDYQKAVDDYLKAASIFKKYKHKT
                     EASAYDALAICFEDISNIPKAVEYSDKAIAAAKKFDDKRILANTLMNRASKEMNWNNF
                     KAADSILNIAGPIVFKLDNTKSYSMYYNNKGDIEAYFKNNLTRALEYYNKSYDYAKLT
                     GSPDLILTPLQSLVSIKMDNNYPDTKQTLDLYYKTASENDFLIETSIALDFYADWYEK
                     QGDYKMAYEYVRKFVKIKDSLTAAETKEQTSLMEVRFRVANKEQEIKQLKNEQKIQHL
                     TIRQKNAINYILIGSVVALLSVLLLTYRIYKNRQLIQQQRISELEKEKQLQATEAVLK
                     GQEEERSRIAKDLHDGLGGLLSSVKYSLNDMKENVILSADNAVSFTRSIDMLDSGIQE
                     LRRIAHNMMPENLMKFGLDKALADYCQSISKTNIVEISYNSFQLEDYIENSNVSITVY
                     RVIQELINNTIKHANATQIIVQVFKENNILNITVEDNGKGFDVNNISNFKGAGWTNIQ
                     NRINYLKGKIQVDSAPTEGTSVNIEIPLT"
     gene            5974..6591
                     /locus_tag="PLL59_08890"
     CDS             5974..6591
                     /locus_tag="PLL59_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMY23965.1"
                     /translation="MKQTKLFIVDDHYMIIEGIISLMQHQSDIEIVGHAQNAESCMAF
                     LQRIKPDVIFLDINLPDKSGIDVCKEIKSSYPAIKIIALSTFNQASYINNMIDNGANG
                     YLLKNATKEEMLLAIQTVMMHKQYLSKEASDVIRETKQQKPILTRREREILKLIAEGL
                     TSSDIGKQLFISTTTVDTHRKHILEKLKANNTAILIKTATQLGII"
     gene            complement(6588..7172)
                     /locus_tag="PLL59_08895"
     CDS             complement(6588..7172)
                     /locus_tag="PLL59_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMY23966.1"
                     /translation="MLVVISRLVLMAKGWTYNVDSVKSIDKCVLIVAPHTSAWDMVLG
                     KSIFTLSNIPAKIAVKKEAFFWPLNWFLRYLGAIPIDRTPKGANSQRISLVDAMANSI
                     KKEKRICMVITPEGSRGKRKQWKTGFYHIAVKANVPIALGYLDFDVKEGIIDKLIYPS
                     GDMDKDMREIMAYYKDKIHLGKYPNRCVTDERWS"
     gene            complement(7246..7827)
                     /locus_tag="PLL59_08900"
     CDS             complement(7246..7827)
                     /locus_tag="PLL59_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMY23967.1"
                     /translation="MIRWIFKLIFKLLGWKLKTEAITGIKQSITIAAPHTANMDLPIA
                     KAAFDLMGLPLRFTVKQEWFRFPFKGIMTELGGIAIDRSPKKPGEPRPSMVEAMVNLF
                     KENPELHVMVTPEGTRSLRTEWKTGFWYTAKQANVPILLGYLDYKNKIAGIGKIIYPT
                     DLETDMRTMMEFYKDITPCHPEKFSLDHRYAPK"
     gene            8206..9435
                     /locus_tag="PLL59_08905"
     CDS             8206..9435
                     /locus_tag="PLL59_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HMY23968.1"
                     /translation="MSNFIKPFEDKAPEIVFEWNDSKTDAQGWLVINSLRGGAAGGGT
                     RMRKGLTKHEVISLAKTMEIKFSVSGPAIGGAKSGINFDPNDTRKREVLERWYKTVAP
                     ILKSYYGTGGDMNIDEIDEVIPITEEYGIWHPQEGIVNGHYHPKERDKINKIGQLRVG
                     VSKLIEDEDYTPSKKEGYLVADMITGYGVAESVKHYYNIYKNEDIKGKKATIQGFGNV
                     GAAAAYFLAQQGVKIAAIIDITGILISKNGLDFEQVKKLFLQRKNNKLSHPDLLPFDK
                     AQAQFWNTKADIFIPAAGSRFVNEDNLTAMQNAGIEVISSGANVPFNDPEIFYGKVLN
                     KADSSLAVIPDFIANCGMARVFAYLMHKNVKITDDAIFQDVSKTIYNALVKVYEKDNR
                     TIYLTKNALQIAIDELS"
     gene            9473..10630
                     /locus_tag="PLL59_08910"
     CDS             9473..10630
                     /locus_tag="PLL59_08910"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HMY23969.1"
                     /translation="MPIYHQLGKIPAKRHVVFKSDAGNYYYEQLFGTEGFHGHSSLLY
                     HIHRPTQVKEILQSKNIAPKIAIEKNIKSLLLEGFKIKAEDDFLESRKALMLNSDCVI
                     GLAAPRKSLTNYFYKNADADELIFIHKGSGMLRTFMGNIPFKYGDYLIIPRGMIYQIE
                     FDSSDNRLLYLESYSPIYTPKRYKNASGQLLEHAPFCERDFKLPMQLETHDEKGDFLI
                     KIKKQNTLHDFVYATHPFDVVGYDGYNFPYGFSIHNFEPITGRVHQPPPVHQTFETNA
                     LVVCSFVPRLYDYHPQAIPAPYNHSNIDSDEMLYYVDGDFMSRNNIEQGHITLHPKGI
                     PHGPAPGAMERSIGQKDTHELAVMIDTFKPLMLTDEAMKIDDGKYYKSWCE"
BASE COUNT         3800 a         2069 c         1813 g         3251 t
ORIGIN      
        1 ataatcatac tcatacagcg atacaatttc atacgctttt tctggcgata aaaattcttt
       61 aatgacattc aattttattt caggattgat gtctttaatt cgctccgcca tccaatcggc
      121 tttgctcaca ccgtgtgtag aatataatgc cggcaactgt cggtttctat tgcttggatc
      181 cactacatct ccatcaacaa tagtcatggt gcccacacca gcacgcgcaa taaactcggc
      241 agcaaaggag ccaataccac ctaaaccaac taccaacacg tggctgttaa taagcttgtt
      301 taagttgtca tcaccaatta acgcagtggt tctcgaaatc cactttttat cctgcatcta
      361 taaaaatttg tgcaaaggta acaaagattt tcttttcgta tttgaaggat tattttgaca
      421 atctatttaa cttgtatgcc aatctttagt tgatgttaca gcattttccg tctgataaaa
      481 taatttgcgg tgtagatgaa gcaggacgcg gttgcttggc tggtcctgta gtggctgctg
      541 ccgttatttt accaaaaaat tttacacacg aatttttaaa cgactcaaaa cagctttcgg
      601 aaattcagag aaatgagttg cggccaatta ttgaaaaaaa tgcgattgct tttggcgttg
      661 gaattataga taataataag attgatgaag taaatatttt acaagcaact tatttagcta
      721 tgcacgaagc cattgcacaa ataaaacaga aaatagattt attaattatt gatggcaatc
      781 gctttaaacc ttataaaaat attgcacacc aaaccatcat taaaggcgac ggaaaatata
      841 ttgaaattgc ggcggcatct attttagcaa aaacctatcg tgatgaaatt atggaaaaac
      901 tacatattga atttccggaa tatcattggc ataaaaacaa aggctatcca acgattgctc
      961 accgcaatgc cataaaaaat ttgagtgctt gtgtttacca tcggcagaca tttagattat
     1021 taaaagaaga acaattaaat ctgtttgaat aaaaaaaagt ccaccaaaaa tagtggacta
     1081 acttcttaat aagaattagg ctttctgtaa tcgtatcaat cttttataca acgcacagaa
     1141 tacccatctt tcatataggt tgttgttaaa taaccatcac aacgatcata atttaataca
     1201 caccaccaag catcagttgc ggtatgatta gcatttgacc aaaatccacc aatttcatta
     1261 aatgttttgt agccacccca aaatccatca tgcttaccag caggaaaccc actaaatcca
     1321 ctactattac tagccccaac atttggactt ttccaaaata catctgtcgc tttcatttga
     1381 caaccagcat aattcgttcc gcctaaataa ttaaatagaa ctgtccattc tgcaattgaa
     1441 ggaacatgcc aaccagtagg tgctaatttt cctgtatata ctgcatacca attgtataat
     1501 tttccataca ctgcatcatt cccaggatta ttgttacaat gactatatac accttctaat
     1561 gtttgtgtat attctaattc agacaaacct gttttaatag gcgagccatc attatatcgc
     1621 gttgtattca aattttccac catccaacat tgagtgccaa tagttacagt atgatacaca
     1681 tttccatcaa tgtccgttac tgttccacaa ttaggaaaag cttctgttgt aaaactaatt
     1741 tcatttccat aggcagtacc tacactattt gtggcataag ctttaacata atatttagta
     1801 ttgcttttta aattagatag gttacttatg aaatttccta ttccattgct atcagtagtt
     1861 ttgttattta atatagttgg attaggattc gtactccaac aaatacctct tgctgtaatt
     1921 atactgttgc cagaattact aatattgccc cctgagacgg ctgaaaactt agcgatactg
     1981 ctcactgttg acgtactaac agttggtaat gtaacaggaa gctctgtaac cactatataa
     2041 cctgactttg ttttagtatt gctgccatct gcattagtaa cagtgagagt aacattgtat
     2101 gatccaacag catggtattg aatatttgtt ggatgttgga gtgtagaagt agatggtgtt
     2161 ccaccctgaa aactccagga ccaagatgtt ggatttccag tagattcatc agtaaaattt
     2221 acagctgaat ctgttcgtat tgccaaacta tttgctgaga aatcggcaac aggtaggaca
     2281 ggcgtatttt tcgtacacga agcaactagc attcctagaa taagaagggc aaatgtgcta
     2341 tgttttgaaa ttttcataaa aacagattaa atcgatgatt aatttttggc ataaaataca
     2401 ttgttgtgat accaaacgcc attaatattt acaccattcc aatcatttgc tgatgggcta
     2461 gatgcattac catcgccatt ggtatctcca ccacgtgtat catctcttaa cgaagtaaat
     2521 attgcagaag gatggatggc aaaatttgcc gtgtacccac taatctcttc accagaacct
     2581 gcaggaattt tgattataac attagcacca acactaaata aattaccaga acctccagca
     2641 cttaaacctt gtaataacac ataaggtact tcagtctccg tatatggaac cgttttgccc
     2701 gataaaccaa atgaatatac atagatacca ttgtacttgt ttgtttcgct cggattatta
     2761 ggattgtgat aaatattatt ttggtctata gaataataac tattgatata tattggaatt
     2821 gcattatcat agaaagcaca attcgtgata acacttacgg aagggtcttt tgtgtcgccg
     2881 ccattaaaag cagcagtgtt gggtgaatta gttccataag tatgtgcaaa ggtacaataa
     2941 tcaaatgtaa agtcgtggat atttcctgta cctacattag ctcttaccac cgagccacct
     3001 tgtccgccac catataagaa tttgcaatat ctaaacacag aaccatgttg atcgccacgc
     3061 atatttatat aagcccaatc tcctttgttt gctgtagttg cagcaccatc tccattatta
     3121 tcgccacaat agctatcatc tttatatgat gtaaaaatga ttggattagc agctgttgca
     3181 ttggcagtaa ctttagcgta aacctccaat ccgcaattgg catttttaaa tttaactatt
     3241 acaccaggct gtatggttaa aggggctttt atattaatcc agttatttat gatatacaca
     3301 ttgccacttg tccatgtagt aactgctgta atattgcttg aaatttcaat ttcattacaa
     3361 gcagctacag catctgttgt aatagtaact tgattaccat aagccacgcc actcgaattt
     3421 tttgcatacg ctcttacgta atatgtatag cctcctgcaa atggcgctac ttctgtcgaa
     3481 aaactgcctg ttccatctgc aattgcaatt gttgccccat ctggatcatc gataaccgga
     3541 ttggctgtca aagcaatggc tacgccattt tccgtcacag cagaactgcc ttgattagtt
     3601 acattgccac ctaatgtata ggtagtgtca tctactttga ttggcgtagc tgtagaaact
     3661 gtggcaatgc catctggagt cgatgaatct ggtgtgcaag cattgagcaa gcaaatcaga
     3721 gtaaataata atgctgcttg cattttaaag ttcaatgtct ttttcatatt aatgttttag
     3781 ttgtttaaga attatgatac aaacttatat cttaaacaaa cttgttttta tcctttaaaa
     3841 tcatgatttt ttgaaaatga aaaatcctat atttatagga tatgatataa aaatatttca
     3901 cttcatattt acatcattaa aattgtaaat cacggaacag aaataggtat acaatattat
     3961 tacttactat aagattgaca gttaaacgaa tcagaaagga aattaaatat acaagatgaa
     4021 aaaaacagca ttattgatct taagttcatt tgtatttatt actacttatg ctgagatttc
     4081 gtataaagac agtttgcgca atgcaatttc aaaagcgccg acagatagtc tgcgtataga
     4141 gtctatgttt caatacatga aagcaacttt aaacgacaat acgagcgact ttgagccata
     4201 catcaaagag atgataaagt tgagcaaaaa gataaattat aaatggggct taagtactgc
     4261 ttatgtttta ggtatatctt actatagaca ccaagccgac tatgagacgg cattgtatta
     4321 cgcgtccctt atagaagaat tatgtaaaaa tgatacatca aaagatatta gaataaattt
     4381 ggggcacatg caccaaaata gaggaaactt gttttctact attggcgatt atcaaaaagc
     4441 agtagatgat tatttgaaag ctgctagcat tttcaaaaaa tataaacata aaacggaagc
     4501 atctgcttat gatgccttag ccatttgttt tgaagatata tcaaatattc cgaaagctgt
     4561 agaatatagc gacaaagcta ttgctgctgc aaaaaaattt gatgacaaaa gaatattagc
     4621 taatacatta atgaacagag ctagtaagga aatgaattgg aataatttta aagcagctga
     4681 ctcaatactg aatatagcag gtccgatagt attcaaatta gataatacta agagctattc
     4741 aatgtattat aataacaaag gtgatataga agcgtatttt aaaaacaacc tgacaagagc
     4801 attggaatat tacaataaat cctatgatta tgcaaaacta accggaagtc cagatttaat
     4861 tttaacacca ttgcaatcat tagtatctat aaaaatggat aataattatc ctgatacaaa
     4921 acaaacgctt gacttgtact ataaaacagc ttctgaaaac gactttttaa tagaaacctc
     4981 aatagcactt gatttttatg cagattggta tgaaaaacaa ggcgattaca aaatggcata
     5041 cgaatatgta cgaaaattcg tgaaaataaa ggatagttta acagcagcag aaaccaaaga
     5101 gcaaacatca ctcatggaag tacgtttccg agtagcaaat aaagaacaag aaattaaaca
     5161 gttaaaaaat gaacaaaaaa ttcagcattt gactatacgt caaaaaaacg caatcaacta
     5221 tattcttatt ggaagtgttg ttgcgctatt aagtgtttta ttgctgactt atcgtattta
     5281 taaaaacaga caattaatcc aacaacaacg catcagcgaa ttagaaaaag aaaaacaatt
     5341 acaagccaca gaagctgttt tgaaaggaca agaagaggaa cgcagtcgca ttgcaaaaga
     5401 tttgcacgat ggattaggtg gtttattatc cagcgtaaaa tattctttaa acgatatgaa
     5461 agagaatgtg attttaagtg cagacaatgc tgtttcgttt acacgaagca ttgatatgct
     5521 cgactctggc atacaagagt tgcgcagaat tgcacacaac atgatgccag aaaacttaat
     5581 gaaatttggc ttagataaag cactagcaga ttactgtcaa tccatcagca aaacaaacat
     5641 agtagaaatc agctacaact cctttcagct ggaagattat atagaaaatt ctaatgttag
     5701 cattaccgtt tatcgcgtta ttcaagaact aataaacaac accattaaac atgcaaatgc
     5761 aacacaaata atagtacaag tgtttaaaga aaataatatt ctaaacatta cagtagaaga
     5821 caatggaaaa ggttttgatg tcaataacat cagtaatttt aaaggtgcag gctggacaaa
     5881 tattcaaaac agaattaact atcttaaagg aaaaatacaa gtcgattctg cgccaacaga
     5941 aggcacatct gtaaacatcg aaataccatt aacatgaaac aaactaaact atttatagtt
     6001 gacgaccatt acatgattat cgaaggtatc atatcgctga tgcaacacca aagcgatatc
     6061 gaaatcgtag gtcatgctca aaatgcagaa agctgtatgg catttctaca acgcataaaa
     6121 ccagatgtta tttttttaga catcaaccta ccagacaaaa gtggcatcga tgtatgcaaa
     6181 gaaataaaat caagctatcc tgcaataaaa attattgctt taagcacctt taatcaagcc
     6241 agctatatca acaatatgat agacaatggc gccaatggat atttgcttaa aaatgctaca
     6301 aaagaagaaa tgcttttagc tattcaaacc gtgatgatgc acaagcaata tctatctaaa
     6361 gaagcatcag acgtaattag agaaaccaag caacaaaagc caattctgac acgcagagaa
     6421 cgagaaattt taaagctcat tgccgaagga ttaaccagtt ccgacatcgg caaacagcta
     6481 tttatcagca ccactactgt tgatacacat cgtaaacata ttttagaaaa actaaaagca
     6541 aacaatacgg caattctcat aaaaacagcc actcaactcg gcatcatcta agaccaacgc
     6601 tcatccgtaa cacatcgatt cggatatttt cctaaatgga ttttatcttt ataatatgcc
     6661 attatctcgc gcatgtcttt gtccatgtcg ccactcggat aaataagctt gtcgataata
     6721 ccttctttga catcaaaatc caaatagcct aaggctatcg gcacattcgc ctttacagct
     6781 atatgataaa atccagtttt ccattgctta cgtttaccac gtgagccttc tggcgttatt
     6841 accatacata ttcttttttc tttctttatg gaatttgcca tcgcatcaac caagctaatg
     6901 cgttgtgaat ttgcaccttt cggcgttcta tctattggaa tggcgccaag gtagcgcaga
     6961 aaccaattta atggccaaaa aaatgcctct ttcttcaccg ctatttttgc aggaatattg
     7021 cttaatgtaa aaatcgattt acccaatacc atgtcccatg cagaagtatg tggtgctacg
     7081 attaaaacac atttatctat cgacttcacg ctgtccacat tatatgtcca acctttcgcc
     7141 attaatacaa gcctagaaat tacaactaac atatctgaaa ttaaagtgca aaaataaatg
     7201 aaaaatcaaa cattcttttc ctcacttcta actacctaca tctaactact ttggtgcgta
     7261 gcgatggtct aaactaaact tctcaggatg gcacggcgta atgtctttat aaaactccat
     7321 catcgtgcgc atatctgtct ctaagtccgt cggataaata attttaccaa tacctgcaat
     7381 cttattttta taatctaaat agcctaataa gataggtaca ttcgcctgtt ttgccgtgta
     7441 ccaaaaacct gttttccact ccgtgcgcaa actacgcgtg ccttctggtg tcaccatcac
     7501 atgtaactcc ggattttctt taaacaaatt caccattgcc tctaccatac ttggtcgtgg
     7561 ttcgccaggt ttttttggac ttctgtcaat agcaatgcca ccaagctctg tcatgatgcc
     7621 tttaaatgga aatctaaacc actcttgctt taccgtaaaa cgcaatggca aacccattag
     7681 atcaaatgcc gccttcgcaa tcggcaaatc catattagcc gtatgtggtg ccgcaatcgt
     7741 aatcgattgc ttaataccag taattgcttc cgtctttaac ttccagccca ataacttaaa
     7801 aatgagttta aaaatccaac gtatcataat atcacaaaca tagacatttt attttatttg
     7861 tactgattta tagttacatg gtttccgtgt cataagtcaa aaaattagcg attctcggac
     7921 ttcaagctct cttcattcca agcataaaca catcaatttc tcaagaaata atatcctatt
     7981 ttatggcgtt gcgccgctca cgcacttatc ccaatggcgc cgtgctgtcc gctacaatct
     8041 ttttttagcc acactaaagc taaaacaaaa aaaagtattt ccgctgccat cactaacgca
     8101 taacactttg ctataaaatc agcatggcaa gaaaaataat ttcaaataaa atagttgcta
     8161 taacaactaa aatgattaaa tttaattttt taatagctat gatttatgtc caattttata
     8221 aaaccatttg aagacaaagc tccagagatc gtatttgaat ggaacgactc taagacagat
     8281 gcacaaggtt ggctggtcat caattccttg cgtggcggcg cagctggtgg tggcacacgc
     8341 atgcgcaaag gattgaccaa acacgaagtc atctcattag ctaaaaccat ggaaataaaa
     8401 ttttccgttt ctggtccagc tattggaggc gcgaaatctg gtatcaactt cgacccaaat
     8461 gacacacgca aacgagaagt gttagaacgc tggtacaaaa ccgttgcacc tattctaaaa
     8521 agctattacg gcactggtgg cgatatgaat attgatgaaa tagacgaagt cattccgatt
     8581 accgaagaat atggcatttg gcatccacaa gaaggcattg tcaacggaca ttatcatcca
     8641 aaggaacgcg ataaaataaa taaaattggg caattgcgcg ttggcgtttc caagctcatc
     8701 gaagatgaag attatacacc atcaaaaaaa gaaggctatc tcgttgccga tatgattact
     8761 ggctacggcg tggctgaatc agtaaagcat tattacaata tttataaaaa tgaagacata
     8821 aaagggaaaa aagctactat acaagggttt ggaaatgttg gcgccgccgc tgcctatttt
     8881 ttagcacaac aaggtgtgaa aattgcagca atcatcgaca tcacaggcat attgatatcg
     8941 aaaaatggat tagatttcga acaggtaaaa aagctattcc tacaaagaaa aaacaataag
     9001 ctttctcatc cagatttact gccatttgac aaagcacaag cccaattttg gaacacaaaa
     9061 gcagacattt ttataccagc tgctggctca cgttttgtca acgaagacaa cttaacggct
     9121 atgcaaaatg ctggcatcga agtcatctct tctggtgcca atgttccttt taatgatcct
     9181 gaaatttttt atggaaaagt attaaataaa gcagactcat cattggccgt tatacctgat
     9241 tttattgcaa actgtggcat ggctcgtgtg tttgcctatc tgatgcacaa gaatgtaaaa
     9301 ataacagatg atgctatttt tcaagatgtg tcaaaaacga tttataatgc tttagttaaa
     9361 gtatatgaaa aagataatcg aactatttac ttaactaaaa atgcattgca aattgccata
     9421 gatgaattaa gttgatatag acaaaagaat cacaacaaga aaaaaataaa ttatgccaat
     9481 atatcaccaa ctcggaaaaa ttcctgcaaa gcgacatgtc gtttttaaaa gcgatgcagg
     9541 caattattat tacgaacaac tattcggcac agaaggtttt cacggtcatt cctctttgtt
     9601 gtatcatata catcgaccta cacaagtaaa agaaatattg caatcaaaaa atattgcacc
     9661 taaaattgct atcgaaaaaa acataaaatc tctattatta gaaggtttca aaatcaaagc
     9721 agaagatgat tttctagaaa gcagaaaagc gctgatgctc aacagtgatt gtgtgattgg
     9781 acttgcagca ccgcgcaaat cattaacgaa ttatttttat aaaaacgccg atgccgacga
     9841 gctgattttc atacataaag gcagcggaat gcttcgcact ttcatgggca atattccatt
     9901 taaatacggc gattacttaa tcattccacg tggcatgatt tatcaaatag aatttgatag
     9961 tagcgacaat cgcttgttgt acttggagtc ctattcaccc atttacacac caaagcgcta
    10021 caaaaatgca tcgggtcagc tattagagca tgcgcctttt tgtgaacgcg attttaaatt
    10081 gcctatgcaa ttagaaacgc atgacgaaaa aggtgatttc ctgattaaaa taaaaaaaca
    10141 aaataccttg cacgattttg tatatgcaac gcatcctttt gatgtagtgg gttatgatgg
    10201 ttataatttt ccatatggat tcagtattca taatttcgaa cctatcacag gaagagtgca
    10261 tcagccacca ccagtgcatc agacatttga gacaaatgct ttagtcgtgt gttcatttgt
    10321 acctagatta tatgattatc atccacaagc tattccagca ccttataacc acagcaatat
    10381 cgacagcgac gaaatgctgt attatgttga tggcgatttt atgagcagaa acaatatcga
    10441 gcaaggacat attacattgc atccaaaagg cattccacac ggacctgcac ctggtgcaat
    10501 ggagcgcagc atagggcaaa aagacacaca tgaattagcc gtaatgattg acaccttcaa
    10561 gcctttgatg ctcactgatg aagccatgaa aattgacgat ggtaaatatt ataaaagttg
    10621 gtgtgagtaa tggtatcatg caaaataatt atcgagcata tctataattc ttataccgca
    10681 aaaaaaataa aaattggaaa taaaaaatgt caattacgga cttgaaaaat atttgaagtc
    10741 taactgtcat tctcgcgtaa gcgggaatct cataatacaa tattgagatt ccctttttca
    10801 agggaatgac tttgagaact ttgtaaaata acattgtgtt ctttgtggtt taaaataaat
    10861 taaaaaaata tgtcaacaga aataaaaaat gtcaattacg gacttgaaaa atatttgaag
    10921 tctaactgtc att
//
LOCUS       DAOQQV010000083        10926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2164_length_10926_cov_7.983994, whole genome shotgun sequence.
ACCESSION   DAOQQV010000083 DAOQQV010000000
VERSION     DAOQQV010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2164_length_10926_cov_7.983994"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PLL59_08915"
     CDS             <1..906
                     /locus_tag="PLL59_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY23970.1"
                     /translation="GYSFAVKNANDHIFFGAGIMVDLGKGKELAEDTVAAVVAKPIDT
                     DGDGIYDIDDACPTVAGLAQFNGCPDTDGDGIEDSKDDCPTVPGLVEFNGCPDRDGDG
                     IIDAKDACPDVKGIAKFGGCPDPDRDGDGIINEKDKCPDVAGSFIAQGCPDKDGDGVM
                     DNEDKCPEVAGPASNKGCPEVKEEVKKRLAFAARNIQFETGKATIKPVSFKILDEVAS
                     ILKEYPYYDVNVDGHTDNVGDDAKNLKLSQDRAAAAVTYLTGKGIAASRLVSAGYGET
                     KPVGDNKTAAGRASNRRVEFNLIFR"
     gene            974..1669
                     /locus_tag="PLL59_08920"
     CDS             974..1669
                     /locus_tag="PLL59_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMY23971.1"
                     /translation="MNKSYKQGFTRSERNGILLLCVVIVISIVAKKYVVNYFAIDDTK
                     MSKLDEEKFEQLQRQINDAKISYANNYSAKEEYKATNNFNSKEQSTVINNKFDFKIEV
                     NSATAEEYEKLYGIGKVFAQRIVSFRDKLGGFYSINQVKHVYGIEDSVFQKFKKNLSI
                     KPAKIKKLNINTATFEELTANPYFFSTIAKQIIGYRTKVKPFETIEDIKNLYFVKDHP
                     EHYDKMLPYVAID"
     gene            1929..3077
                     /locus_tag="PLL59_08925"
     CDS             1929..3077
                     /locus_tag="PLL59_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY23972.1"
                     /translation="MNFEYSENQQMIAQTVRDFAEKHIRPYVLEWDETQHFPVETMHK
                     LGELGLLGILIPEEYGGAGLSYYEYITALIEIGKVDSALCLSVAAHNSLCTNHIYKFG
                     NAEQRKKWLPKLASGQWIGAWGLTEANTGSDAGRMKCVAHEDGDHWVINGSKNFITHG
                     ISGDVAVVIVRTGELLDSHGMTAFVVERGTPGFSGGKKENKLGMRCSETAEMIFDNCR
                     VPKENILGNVGDGFIQAMKILDGGRISIAALSISIAVGAYEAAVKYAKEREQFGKAIA
                     DFQAIGFKLADMATEIEASKLLTFQAADMMIRGEKMTKQSAFAKYYASEVACKVANEA
                     VQVLGGYGYIKDFPVEKFYRDAKLCTIGEGTSEIQKLVISREILKGNI"
     gene            3173..3364
                     /gene="rpsU"
                     /locus_tag="PLL59_08930"
     CDS             3173..3364
                     /gene="rpsU"
                     /locus_tag="PLL59_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HMY23973.1"
                     /translation="MLIVDARESESLDRALKVFKKKFEKTGTVKQLRARQAFTLPSVK
                     RRTEIKKAIYVEQLRKGEE"
     gene            3422..4330
                     /locus_tag="PLL59_08935"
     CDS             3422..4330
                     /locus_tag="PLL59_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMY23974.1"
                     /translation="MAAKMIEAFLNYLTFEKRFSNKSVESYKIDLLQFFYFIKNDFET
                     EDLLQIKHQHVRAFVVQLVSANNKPSSIKRKISAIKSFYKYCQKTGKIENNPADKINT
                     PKSPERLPKFIEQQKINHLLEKPDLFFSKENDFDNMQEKLLIDVLYSTGMRRQELINL
                     QWSAIDFSNKQIKITGKGNKQRLMPVAHQLLDDLLQFKQFQREALKNIPIAPYVFLTK
                     DGKQLYPNYVYRIVKMHISHCSTVDKKSPHVLRHSFATHMSNNGAKLNDIKELLGHAS
                     LASTQVYTHNTIEQLKEIYKNSHPKA"
     gene            4457..4756
                     /gene="raiA"
                     /locus_tag="PLL59_08940"
     CDS             4457..4756
                     /gene="raiA"
                     /locus_tag="PLL59_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HMY23975.1"
                     /translation="MKIQIQSLHFDADKSLKDFINKKLVKLETFYDKIIDSDVVLSLE
                     QLNTQVRDKVVVIKTNIPGGTLIAKEKSKKFEAAVDLAVESLKKQLEKIKAKKKE"
     gene            5828..7558
                     /locus_tag="PLL59_08945"
     CDS             5828..7558
                     /locus_tag="PLL59_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23976.1"
                     /translation="MGNNNQDAYNMSILDMSQRLSAIYKNGMFYTDYNINTLIKVKKI
                     KFSSDSIRMLIIQPIYSPTLKKRCVLMSPGNGESFSNWYLSNRYAVDFALRGYVVAYY
                     ENSGSKNPNLNNSGKNTDTYFADKVINPCCVAPNNSIRDKFFSSMFVNLFISNAARKY
                     IVNNSTILNVDTTKFFLAGGSLGANTALFYGYGCNNNWSTNSYYTCVKNMLNYPDTLS
                     NAGVKGIMSMGGGLPAPTEALGDIITNIDYIPSIWFAGAGDIAVNPNESDILGPVIWG
                     ALSMKNELTANSINNNMYLNCFGVHSFESPAYDDPSYGEWTNSLPTNTISGNTNPPFN
                     TTLNLSQVTNYKNAHQQKLNYYQYTEHQSYQALVNTSIYFNNIINNSIPSNLVNYIRP
                     TQLQNSPFYKYSIGGYTLEQAVLSYYTCQKNEPLCIFAGAHEYDNIAKNNLNSTFLPS
                     NECNVLANNFGAEFNSNNSTYSTSRLSKNLEINSENLSIEKLNIILLDNNHIELKIPK
                     TLVNSSGTLNFYNILGLRVGYQNEFKINEEQNLIFDISQTLHHLPNGIYFCVVQLSNA
                     TETLKIVHNR"
     gene            7597..8745
                     /locus_tag="PLL59_08950"
     CDS             7597..8745
                     /locus_tag="PLL59_08950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMY23977.1"
                     /translation="MSKVYFKNLNGIRFILAALVLLHHVAVVMQVCEISNIYQKIGAL
                     KAFGPVAVSLFFTLSGFLITYLLLIEREQSQTISLRTFYIARAKRILPIYYLIVILHL
                     YILPHTPLYAFESKLLLVDVSTYNYSTLVLPEWSINLWYFILLPQVALAIMVASGNAF
                     VPAGHVWSIGVEEIFYFLYPILLKKYAHKFKKFILLLIALYYIISIFIFIGAYCSKSA
                     LITNTLFSRILNFSIIMIIYNRINCMFIGAIAAYLLFKKHALLIKVTKPAVFYSSIIG
                     IIFLFAIGARVPFLTYEVYCLLFVCILLYFIHNNIHTFLEHPLLVYLGKISYGIYMYQ
                     MIAIFFTVYLYKLGYINIFFIFLISFLITIILSAISFELIEKRIMKKR"
     gene            complement(8904..10775)
                     /locus_tag="PLL59_08955"
     CDS             complement(8904..10775)
                     /locus_tag="PLL59_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HMY23978.1"
                     /translation="MQNSGIPISISLDSTYKSEQYDNNNNKQEQLRLLRLSLIAICIA
                     ILVSAIARILVHLIDLFTNIAFRGQFSLEAFSPAQHQLGLFVICIPAFGGLLVGLMAY
                     YGSKAIRGHGIPEAMEQILTNQSKIKPSITYLKPLSSAISIGTGGPFGAEGPIIATGG
                     ALGSTIGQLLHITPNERKILLAAGATAGMAAIFGSPIAAMFLAIELLLFEFSPRSFIP
                     VALACITGAAGHHLLFGEAYVFPITIPIVAASNKALFFYSLMGLIIGLLSVGITKIVY
                     WIEDAFEKIPIHWAWYPAIGGIAVGIIGYFNPKTLGVGYSNITDLLSGTMALQMIISL
                     CLLKFISWAISLGSGTSGGTLAPLLTIGGAIGAIAGNLGNAWFPDFGISISLSALVGM
                     SAMFAGASRAYLTSIIFALETTGQSAALLPLLAACTASYTVSYFLMSNTIMTEKIARR
                     GVYTPHEYGPDLLERTFVSEVAYDSGVVVSNTMSIDEVRNWLKHEENERPNFIIITDE
                     NGIYKGVISSSNLFSLHHQLQNTIESLIKRKDIFILPEDSLKSAVELMTRENMDILPV
                     LDQTAVVGILTYKNMMSIYKKDFVDNAHRQRHISLNRQRLKLLVRGQRLISLLKISK"
BASE COUNT         3839 a         1737 c         1879 g         3471 t
ORIGIN      
        1 ggttacagct tcgcagtaaa aaatgcaaac gaccatatct tctttggtgc tggcatcatg
       61 gttgatttag gtaaaggaaa agaattagca gaagatacag tagctgctgt tgttgccaaa
      121 ccaatcgata cagatggtga tggcatttac gatattgacg atgcttgccc tacagttgct
      181 ggtttagcac agtttaacgg ttgcccagac acagatggcg atggcattga agatagcaaa
      241 gatgattgcc ctacagttcc tggcttagta gaatttaatg gctgcccaga cagagatggc
      301 gatggtatca tcgatgccaa agatgcatgt cctgatgtaa aaggtattgc taaatttggt
      361 ggttgccctg atccagatag agatggtgat ggtatcatca acgaaaaaga taaatgtcca
      421 gatgttgctg gctcattcat cgcgcaaggt tgcccagata aagatggcga cggcgtaatg
      481 gataatgaag ataaatgtcc agaagttgca ggtcctgcat ctaataaagg ttgccctgaa
      541 gtaaaagaag aagtaaaaaa acgtttagca tttgctgctc gtaatatcca attcgaaaca
      601 ggtaaagcaa caatcaaacc agtatctttc aaaatcttag atgaggttgc ttctattttg
      661 aaagaatatc cttactatga tgtaaacgta gatggccata cagataatgt tggtgatgac
      721 gctaaaaact tgaaattatc tcaagataga gctgctgctg ctgttacata cttaacaggt
      781 aaaggaattg ctgcttcaag attagtttct gcaggttatg gcgaaacaaa accagttggc
      841 gacaataaaa cggctgctgg cagagcttca aacagacgtg tagaattcaa cttgatattc
      901 agataataaa tagttgacag tatatataaa aaaaggtctc gaatttcgag accttttttt
      961 atatttgtta ataatgaata aatcatacaa acagggtttc acacgttcag agcgaaatgg
     1021 cattttgcta ttgtgtgtag tgattgtcat aagtattgtt gcgaagaaat atgtggtcaa
     1081 ttatttcgcc atcgacgata caaagatgag taagctagat gaagaaaaat ttgaacaact
     1141 ccaacgtcaa atcaatgatg cgaagataag ttatgccaat aactatagtg ctaaggaaga
     1201 atacaaagcc acaaataatt tcaatagcaa agagcaatcg acagtaataa ataataagtt
     1261 tgattttaag atagaagtaa attctgccac agcagaagaa tacgaaaaac tatatggtat
     1321 cggaaaagta tttgcccaaa gaattgtaag cttcagagat aaattaggag ggttttattc
     1381 tatcaatcaa gtcaaacatg tatatggtat tgaagattct gtatttcaga aattcaaaaa
     1441 gaatttaagt atcaaaccag cgaaaattaa gaagctaaat attaatacag caacatttga
     1501 agagttaacg gcaaatcctt atttcttttc tacaattgca aaacaaatta ttggttatcg
     1561 cactaaagta aaaccatttg aaacaattga agatattaag aatctttact tcgtaaaaga
     1621 tcatccggag cattatgata agatgttgcc gtatgttgca attgattaat gttgttatac
     1681 tctttggaat ttgctcctcg cctttgattt ccctcgcctt tgttggtttt caaccaacaa
     1741 atcactaact gtcgccttcg atggttttca accaacaaat cactaactcg tacaagtatg
     1801 ctgccatgat ttgtgaggaa gcgtacttgt gcagaattaa aactatttag atagtggttt
     1861 attgcttaca aatcaactaa gtagagaccc aacgcttttt ttactatctt tacaaaaaaa
     1921 ataatttcat gaactttgag tactccgaaa accagcaaat gattgcgcaa acagtacgtg
     1981 attttgcgga aaaacatatc agaccttatg tattagaatg ggatgagacg caacattttc
     2041 cagtagaaac gatgcacaaa cttggagaat taggtttgtt aggtatctta attccggaag
     2101 aatatggcgg cgctggttta agttactacg aatacatcac agcattaata gaaattggaa
     2161 aagtggacag tgcgttgtgc ctttcagttg ctgcacataa ttcattatgc acaaatcata
     2221 tttataaatt tggaaacgcg gaacagcgta aaaaatggtt gccaaaatta gcaagtggac
     2281 aatggattgg tgcttggggg cttacggaag ctaacacggg ttcagatgct ggtagaatga
     2341 agtgtgtagc gcacgaagat ggtgaccatt gggtgattaa cggttctaaa aattttatca
     2401 cacatggcat aagtggcgat gtagctgttg ttattgtgcg tacgggagaa ctgttggaca
     2461 gccacggcat gacagcattt gtagtagagc gaggaacacc aggttttagt ggaggcaaaa
     2521 aagaaaataa attaggaatg cgttgctctg aaactgcaga aatgattttt gataattgtc
     2581 gcgttcctaa agaaaatata ttaggaaatg taggagatgg ctttatacaa gctatgaaaa
     2641 ttttagatgg cggaaggatt tccattgctg ctttaagtat aagcattgct gtgggcgctt
     2701 acgaagctgc agtaaaatat gcaaaagaaa gagaacaatt tggtaaggca attgctgatt
     2761 ttcaagctat cggttttaaa ttagctgata tggcgacgga aattgaagca tcaaaattat
     2821 tgactttcca agctgcagac atgatgattc gtggtgagaa aatgacaaaa caatccgcat
     2881 ttgctaagta ctatgcctca gaagtagcgt gcaaagtagc caatgaggcc gttcaagtat
     2941 taggaggtta cggttatatc aaagactttc cagtagaaaa attctataga gatgctaagt
     3001 tgtgtacaat aggcgaaggt acatcagaaa tccaaaaatt agtgattagc cgagaaatct
     3061 taaaaggaaa tatttaacat aaaattctta ttttcaattt gactaatttc ctcattttca
     3121 aattaatcac tatctttgca gtccaatctt aaaaataaaa gaggtataaa ttatgttaat
     3181 cgttgacgca agagaatcag aatcattaga cagagcacta aaagtgttta agaaaaaatt
     3241 tgaaaaaaca ggcactgtaa aacaattacg tgctcgtcaa gctttcactt tgccttctgt
     3301 aaaaagacgc actgaaatta aaaaagctat ctacgtagag caacttcgta aaggagaaga
     3361 ataatagttg cgcttttcat tttttttgtg taatttaatc tttctaattt agcgaagatt
     3421 aatggcagcg aaaatgatag aagcgtttct gaattacctt actttcgaaa aacgattttc
     3481 caataaatct gtagagtctt ataaaataga ccttctacag tttttttatt ttataaaaaa
     3541 tgacttcgag acagaagatt tattgcaaat aaaacaccaa catgttcgag catttgtagt
     3601 gcaattagta tctgccaaca ataaaccttc cagcattaaa cgaaagatat cagctattaa
     3661 atctttctat aagtattgcc aaaaaacagg aaaaattgaa aacaatcctg cagataaaat
     3721 caatacgcca aaatcacccg aacgcctgcc aaaatttata gaacaacaaa aaatcaatca
     3781 tcttttggaa aaacctgatt tgtttttttc taaggaaaat gattttgata atatgcaaga
     3841 aaaactactg attgatgtat tgtattctac tggtatgcgc cgacaagaac tgataaactt
     3901 acaatggtcg gctatagatt tttctaacaa acaaataaaa attacaggca aaggcaataa
     3961 gcaacgtcta atgcctgttg cacaccaatt gcttgatgac ttgctccagt ttaaacaatt
     4021 ccagcgtgaa gcattgaaaa acattcccat tgcaccttat gtttttttaa ccaaagatgg
     4081 gaaacaactg tacccaaatt acgtttatag aatagtgaaa atgcacatca gtcattgtag
     4141 tacagtcgat aaaaaaagtc cgcatgtttt acgccattct tttgcgacac acatgagcaa
     4201 taatggtgca aaattgaatg atatcaaaga actattgggt catgcaagtt tagcttcgac
     4261 acaagtttat acacacaata ctattgaaca attaaaagaa atctataaaa attcacaccc
     4321 aaaagcttag cctatgacaa atctttaaat cagttttttt gacaattatt ggatattaca
     4381 atgaagatga aacgatgaat accaataatt ttgaaccgat aacttattac tcaatttata
     4441 aacttttttt atgtcaatga aaattcaaat ccaatctttg cactttgatg cagacaaatc
     4501 tctaaaagat tttatcaata aaaaattagt caaattggaa acgttttacg acaaaattat
     4561 agactctgat gtcgtattaa gcttagaaca actcaacaca caagtaagag acaaagtagt
     4621 tgtcatcaaa acgaatattc ctggcggaac gcttatagca aaagaaaaaa gtaaaaaatt
     4681 tgaagctgca gtagatttag cagtagagtc attaaaaaaa caattagaaa aaattaaagc
     4741 caaaaagaaa gaataatatc acagaaatta taaatacaga ttgagggctc gccgtaggcg
     4801 agccctcaat ctgtataata cgggcataat actgaaaaat agttggtaag ctagttcaaa
     4861 gcatcaatga ttattattgt tttgagataa ttctattcag tggtttgtgt cctcacaaac
     4921 cacttttctt gaactgtaac aatacgaacc acgtagcttc tcagcagagt ctcctgaaag
     4981 ggactctgtg taaatcaagg tagtacatac tatttctctg agccctcttc gagagacgct
     5041 gctagaaaag gaaaatagta caacaaattc tacttagtgg tttgtgtcct cacaaaccac
     5101 tttttttgta atgtatcctt acacaaactg atttttaatt attagcttct cagtagagtc
     5161 tcctgaaagg gactctgtgt aaatcaaggt agcacatact atttctctga gtcctcttcg
     5221 agagacgctg ctagaaaagg aaaaaagtac aacaaattct acttagtggt ttgtgtcctc
     5281 acaaaccact tttttttgaa atgtatcctt acaaactgat ttttaattat tagcttctca
     5341 gcagagtctc ctgaaaggga ctctgtgtaa atcaatgaag tacataataa ttttgcagag
     5401 tcctcttcgc gagacgctga gaagaaaacc gttcgcaacg cattatattt gatggtttct
     5461 tagctgattt ttgacaaaac atgttatttt tttatttttt attacaataa ttccaatttt
     5521 gataattttt tacattatta ttttccactt aatctactta taatcaatat gttagtatgg
     5581 tattattgca tttttttgct ttataaattc tgacaaagcc aaatatttgt accagataat
     5641 tataaaaatt ttgtttatta ttgttgcaat aaaatatatt tattatgaat tatagtttaa
     5701 atctgcgtat atatatatat atatatatat atcaatctct ttaatttgtg taacttatgc
     5761 acagtacggt acgacataca attacatcaa cgtaaataca caagccttca ctgtaccacc
     5821 cagttttatg ggaaataata atcaagatgc atacaacatg agtattttag atatgtcaca
     5881 acgattaagt gccatttata aaaacggcat gttttataca gattataata tcaatacact
     5941 aataaaagta aagaaaataa aatttagttc tgatagtatc agaatgttga ttatacaacc
     6001 tatatatagc ccaactttga aaaaacgctg tgtgttaatg tcacccggta atggagagtc
     6061 ctttagtaat tggtatctat caaatagata tgcagtagat tttgcactac gtggttatgt
     6121 agttgcatat tatgaaaact ctggttctaa aaatccgaat ttaaataatt ctgggaaaaa
     6181 tacagacact tattttgcag acaaagttat aaacccttgt tgtgttgcac ctaataattc
     6241 aataagagat aagtttttta gttcgatgtt tgtcaatttg tttataagta atgctgcacg
     6301 caaatatata gttaataata gtactatatt aaatgttgat acgacaaaat tctttttggc
     6361 aggaggtagt cttggcgcca atactgcttt gttttatggt tatggttgta ataataattg
     6421 gagtacaaac agttattata catgtgtaaa aaatatgctt aattatcctg atactttgtc
     6481 aaatgcggga gtaaaaggta tcatgtctat gggaggtgga ttaccagcgc caactgaagc
     6541 acttggcgat attattacaa atatagatta tataccaagt atatggtttg ctggagctgg
     6601 tgatatagca gtaaacccaa acgaatccga tatcttgggt ccagtaatat ggggcgcatt
     6661 atcaatgaaa aatgagctaa cagctaatag tatcaataat aatatgtatt tgaattgttt
     6721 tggtgttcac tctttcgaat ctcctgctta tgatgatcca agttatggtg aatggactaa
     6781 ttctttgccg actaatacaa taagtggaaa tacgaatcca ccatttaaca caaccctgaa
     6841 cttatcacaa gtgacaaatt ataaaaatgc acatcagcaa aaattaaatt actatcaata
     6901 tactgagcat cagagttatc aagcattagt aaatacttcg atatatttta ataacataat
     6961 aaataattcg attccgtcta atttagttaa ttatataaga cctacacaat tacaaaattc
     7021 cccattttat aagtatagta ttggtggata tacgcttgaa caagcagtat taagttatta
     7081 cacttgccaa aaaaatgaac cactatgtat ttttgctggc gcacatgaat acgataatat
     7141 tgcaaaaaat aatttgaact caacattctt accatcaaac gaatgtaatg ttttggctaa
     7201 taattttgga gcagaattta atagtaataa ttccacatat tcaacaagtc gactatcgaa
     7261 aaacttagaa ataaattcag aaaatttatc tattgagaag ttgaatatta tattactcga
     7321 taataatcat attgagttaa aaataccaaa gactttagtt aattcttcag gcacattgaa
     7381 tttctataat attttaggct tgagagtcgg gtatcaaaat gaatttaaaa taaacgaaga
     7441 acaaaatttg atttttgaca tttctcagac actacatcac ttacctaatg gtatttattt
     7501 ctgcgttgtt cagttaagta atgcgacaga aacattgaag atagttcata atagataatt
     7561 tcttaaacat taatacgtat ctcaacaata aatagtatgt caaaggtata ttttaaaaac
     7621 ttaaatggta ttcgttttat attggctgct ttggtattac tacatcatgt tgctgttgta
     7681 atgcaagtat gtgaaatttc gaatatctat caaaaaatag gagcattaaa agcttttggt
     7741 cctgttgctg tttctttatt ttttacacta agtggctttt taataacata tttattacta
     7801 attgaaagag agcaatcaca aacaatatca ttacgaacat tttatatagc acgtgcaaag
     7861 cgtatcttac caatttatta tctaatcgta attttgcatt tatatatttt gccacataca
     7921 ccattgtatg cttttgaaag taaattatta cttgtagacg taagcacata caattactct
     7981 acattggtgc ttcctgaatg gagcataaat ttatggtatt ttattttgtt acctcaagtt
     8041 gcattagcaa ttatggtcgc atcaggtaat gcatttgtac cagcaggaca tgtttggagt
     8101 attggtgttg aggaaatatt ttatttttta tatcctatat tattaaagaa atatgcacat
     8161 aaattcaaaa agtttattct tttattgatt gctttatatt atataatttc aatatttatt
     8221 tttattggcg cttattgttc caaatctgct ttaattacaa atactttatt ttctcgaatt
     8281 ctaaatttca gtataataat gattatttac aatcgcataa attgtatgtt tattggagct
     8341 attgctgcat atcttttatt taagaagcat gcactattaa taaaagtaac aaaaccagct
     8401 gtgttttata gttctattat aggaatcata tttttatttg caattggagc tcgtgttcct
     8461 tttttaacgt atgaggttta ttgtttatta tttgtatgta tattattata ctttattcat
     8521 aataatatac atactttttt ggaacatcca cttttggtat atttaggtaa aatatcttat
     8581 ggtatctata tgtatcagat gattgctatt ttttttactg tttacttgta taaacttggt
     8641 tatataaata tattttttat ttttttgata tcttttctta taactataat attatctgct
     8701 atttcatttg agttaattga aaaaagaatt atgaaaaaac gttgagttta aatattcttc
     8761 atatcaatcg gttgtacaga aacttacata gcttatcaac agaacctcct gaaagggact
     8821 ctgtgtaaat caatgaagta cataataatt ttgctgagtc ctcttcgtga gacgctgcta
     8881 gaaaacgaaa aaagtactgc aaattacttg cttattttta ataaagaaat caatcgttga
     8941 ccacgaacta ataactttaa acgttgccta tttaatgaaa tatgcctttg tctgtgggca
     9001 ttgtcaacaa agtctttttt gtatatcgac atcatgtttt tatatgtcaa aatacctacc
     9061 actgctgttt ggtctaatac aggtaagatg tccatatttt ctctagtcat cagctccaca
     9121 gcacttttca aactgtcctc tggcaaaata aaaatgtctt ttcttttaat caaggattca
     9181 atagtatttt gtagttgatg atgtaaacta aataaattag aactgcttat tactcctttg
     9241 taaatgccat tttcatcagt aatgatgata aaatttggac gttcattttc ttcatgctta
     9301 agccaattgc gcacttcgtc aatactcatc gtgttactca ccacaacgcc gctatcataa
     9361 gcaacttcac ttacaaaagt cctttccagt aagtcaggtc cgtattcgtg tggcgtatat
     9421 acacctcttc gcgcaatttt ttcagtcatt atggtgttgc tcatcaaaaa gtaggaaaca
     9481 gtatatgagg cagtgcaggc agccaaaaga ggaagcaatg cagctgattg tccagtggtt
     9541 tccaaagcaa aaataataga ggtaaggtaa gcacgggaag caccagcaaa cattgccgac
     9601 atgcccacta atgctgacaa tgaaattgaa attccaaagt caggaaacca agcatttccc
     9661 aaatttccag caatagcacc aatggcacca cctattgtaa gtaatggtgc taaagtacca
     9721 ccagaagttc cactacctaa tgagattgcc cacgatataa actttaataa acacaatgaa
     9781 ataatcattt gtaatgccat cgtgccagat aataagtctg tgatatttga atagccaaca
     9841 cctaatgttt ttgggttaaa atatccaatt attcctactg caatgccgcc tatcgcagga
     9901 taccaagccc aatgaatagg aattttttca aatgcatctt caatccaata tacaatttta
     9961 gtaataccca ccgatagcaa tcctataatt aagcccataa gactgtaaaa aaataaagct
    10021 ttatttgatg cagcaacaat aggaattgta attggaaata cataagcttc gccaaataac
    10081 aagtgatgcc cagctgcgcc tgttatacat gctaatgcta caggaataaa tgaacgtgga
    10141 gaaaattcaa acagcaataa ctcgatggct aaaaacattg ccgcaatcgg actgccaaat
    10201 attgcagcca taccagcagt agcaccagca gcaagtagaa ttttacgttc atttggagta
    10261 atgtgtaaca attggccaat tgttgagccc aatgcaccac cggttgcaat aataggacct
    10321 tcggcaccaa aaggtccacc ggtgccaatg gaaatggcag atgataatgg ttttaaatag
    10381 gtaatagatg gcttaatttt actttggtta gttagaattt gctccatagc ttcaggtatt
    10441 ccatgaccac gaattgcttt tgatccataa taagccatta aaccaactaa taatccgcca
    10501 aatgcaggaa tacagataac aaataagcct aattgatgtt gcgcaggaga aaatgcttcc
    10561 aatgaaaatt ggccacgaaa tgcaatattg gtgaacaaat ctataagatg tactaatatt
    10621 cgcgcaatcg cactgacgag tatcgcaata cagatggcaa taagagatag tcgcaatagt
    10681 cgtaattgtt cttgtttatt gttgttgtta tcgtattgtt cgcttttgta ggtagaatct
    10741 aaagaaatag aaataggaat ccctgaattt tgcatgtatg agcgtgtttt tgcaaaagta
    10801 gttgttgaaa tataaagcat atagtcgtta acaaaaaatt aggataataa cccgttgtgc
    10861 ggtttgaaaa ttataatctt caataaaaaa caacggaaag ttaaacttag taggatgtaa
    10921 agaagc
//
LOCUS       DAOQQV010000084        10798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2201_length_10798_cov_7.673741, whole genome shotgun sequence.
ACCESSION   DAOQQV010000084 DAOQQV010000000
VERSION     DAOQQV010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10798
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2201_length_10798_cov_7.673741"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1165
                     /locus_tag="PLL59_08960"
     CDS             164..1165
                     /locus_tag="PLL59_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23979.1"
                     /translation="MQIQHISLLLNIPLLLIGFFLLNRGAGMLVDGASSIARRLHFPE
                     TMIGFTIVAFGTSLPKLFTVLLAAYNGYSDLVIGTIIGSCIFNLIGILSITGFFRPIR
                     SNKYTASIGLLFLIVAIALFFLVANKQLMPLDAPAPTLLITRTEAIVLLSFFVIFMLY
                     GYFNVQRHHKIWFDEIAQTHDLTYYAWWISLLLILIGIVGLVAGGVLVVNNLIDISRK
                     FDLSQRFLGQFVLSFGGSAVMLYWLMITHQNKSKFELSNLVGQNVLNIVGMLGVAACI
                     QPIVYNPIFNLDIYILIAIVFIVSGLLWMGKKLTLNIWKCRLLFLLLLIYIAYLFMR"
     gene            complement(1166..1597)
                     /locus_tag="PLL59_08965"
     CDS             complement(1166..1597)
                     /locus_tag="PLL59_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HMY23980.1"
                     /translation="MKGNIDFYKKVFNEIIPFNKYIGLELVHIEAGFAKATIQFKQEL
                     VGDIRIQAIHGGVISAAMDAVGGVAGMTTLTSVEDKIVTVDMRVDYIRSARNTDLRIE
                     ANIVRSGNKIITTNMQVFAVNDNILVAEGRGVYHVSRKVKE"
     gene            complement(1611..2747)
                     /gene="hemW"
                     /locus_tag="PLL59_08970"
     CDS             complement(1611..2747)
                     /gene="hemW"
                     /locus_tag="PLL59_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HMY23981.1"
                     /translation="MAGIYLHIPFCKQACSYCNFHFSTLLKYKEDVLQAMLLEIARQQ
                     DFFIEKTNINTIYFGGGTPSILSAGDINRLLAQIHKYFDVQINAEITLEANPDDLTLE
                     YLLALKKETPINRFSIGIQSFVDADLQYMHRAHNAAQAKACVEHAQHVGFHNLSVDLI
                     YGTPTLNATQCLQNIQTVIDLQVPHVSCYALTVEEKTELASLIKKRKKEAPTDEAMTA
                     QFDILTAQMRAHHYIHYEISNFGKEGHFAQHNTNYWKGIPYLGIGPSAHSFDGQKRYW
                     NIANNAIYLKKIKTNEAHFDSELLTETDKYNEYIMTSIRTIFGVDIRKLQHDFEEKYV
                     QHFLREIKLFKTKNWIEENNGIYRLTDKGKLFCDHISAHLFIEN"
     gene            2770..4545
                     /locus_tag="PLL59_08975"
     CDS             2770..4545
                     /locus_tag="PLL59_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23982.1"
                     /translation="MVNIDNFDKEDTSHLHFYCLQIALFLFFAHSVTAQNIRLFIHKQ
                     NEQFKIKEKYTFKNEKELHQYLSATQQKLIKQGYVEASFDSTFSRNDSVFTFLHFGNK
                     VIINNPSVVNKKRKSKNHTETSIFDIDKNKENILEQLENNGYPFAKISTDSLVLSDST
                     LHFKFNIDKGKLITIDSFVNHGTSKITKGFIQNYILIKSKQPYPESRIQQIDKLLQKL
                     PYATLRQPTNVLFRDNKADVHLYLDKRKVNSFDFLIGFLPGSNRGKILITGEVRIHLQ
                     NSFKRGEEIYIEWRKLQRQSQLLDVRFNYPYLLNAPIGVNFTFYLEKRDSSSLDLTWQ
                     LGLPYITKSNNYIKGFYKYFQTIVLAADTAFAKLNKKLPNNLDATYHQYGVQAYYENL
                     DYLFNPKKGFELKLTGSIGTKKIKPNQQITTLQDAFNFNYESLYDSIKRRTIKGDLFW
                     TGNYFLPLGKKQRHVLKFGIDGGAVFNQNLLRNELLRIGGSRSLRGFDEQSILASTYN
                     MMSLEYRMIMMRNSYFFLFIDAAYVHRKFNSTTFQDFPFGFGTGVTFATKIGIFGLTY
                     ALGQQKDKPIDFRSSKLHFGYVATF"
     gene            4529..5398
                     /locus_tag="PLL59_08980"
     CDS             4529..5398
                     /locus_tag="PLL59_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HMY23983.1"
                     /translation="MWQPSKLVLLFIGLFLLFSSTFAQATATNINPFFRDSILKHKVD
                     TARWSNVESAVNRYNKSATNIRLQKPKVETNSLLYLYMTAGIVILLIIMRLIFDDFST
                     SLLEGMLSMKKFFVFYKSKKYDSLIAIILVYVFNLVLLSLISFIAWSFTTKHGFKTFN
                     MSVFMDISVLMIVFFTLKNAIEYLFNFIIDTQDTFKAFFLQNLFTEFLLCVVLLVFSL
                     IYIYNSYFSLNWMLGLFFVILTIYLVFNTIRSYQLISVTRLPYKLHFFLYICTFKLIP
                     ILLLVKYFLNNIV"
     gene            5412..6479
                     /locus_tag="PLL59_08985"
     CDS             5412..6479
                     /locus_tag="PLL59_08985"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HMY23984.1"
                     /translation="MAKKLAAKQTSVKKTSTAKTTKKPAVKSSVSKATKKETKPAKKV
                     EKPVAKPTKAIAAKKTSKVAAPKIVVEKPVAVATKEKDTKLDKKVIIEAKPLPAIVKP
                     NTLKPVKSILISQPKPELGKSPYIDLAQHFKIKLDWRPFTHVEGIKALDFRKQKIYIE
                     QYACVIFTSRIAIEQYFRICEELRIKISEENKYFCISEAIALYLQKFIQYRKRKVFFG
                     DGKLPKLMEVILKHKENGKFLLPCSDVRDKALAEALNKYNIEYSEAFIYKTVASDLSD
                     LSDVKYDMLVFFNPSAIDSLYTNFPKFSQDETRLAIFGNTTADAVKAHKLNINVFAPT
                     PENPSLVDAIKKYITEANKKR"
     gene            6570..7541
                     /locus_tag="PLL59_08990"
     CDS             6570..7541
                     /locus_tag="PLL59_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMY23985.1"
                     /translation="MPLRFNQLILLLCCTISSSIFAQIQPATSHYMFQPQVANPAFYG
                     TKNGINFGANYRHQWAALNGQPRTINAFADASIPAAHGGVGINLSNDMLGAYNILNIN
                     AGYTYIQALPKGIKLNIGLNIGATFSKLDGAKLTTPQGIPGDLNDDILSNQVQKSTRP
                     SITIGLALTHKFLEFGIAYTNAIQSKDKFKGENKTEALRYGATLQTYISSKIKIKADF
                     FIRPSILLATDFKELQTEISVLGGYKNYITLGFNVRGYNKKAFESLSPIIGVGPLKNI
                     CIYYSYDVSLSHLKSVNKGSHEISLNYLLPNNKIYKNPKMINNPRFL"
     gene            7646..8947
                     /locus_tag="PLL59_08995"
     CDS             7646..8947
                     /locus_tag="PLL59_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMY23986.1"
                     /translation="MNKLKFSFIAVIAVLLASCKGGNGTDGQLIGSQDRPKWDNNLLP
                     YGMIYVPSGVFHTGPSDQDVNYAFNTKMKQLSIVGFYMDETEITNNEYRQFVEYVRDS
                     IAHQMLGGDHLKEGDNGKQAINWDMPIDMDWSNGSSVTEEGAQLDPMFYSEADRLNGR
                     KEVDPRKLEYEYTWFKWKEAAQQRPTDKDVKPRSAFMEKKKISIYPDEMVWIRDFSYS
                     YNEPMTRSYFTHPAYDDYPVVGVTWDQGNAFCAWRTLMWNNYRMSQKEAPLDEFRLPT
                     EYEWEYAARGGRNLSPYPWGGPYVRNTKGCLLANFKPGRGNYPEDGGFYTVNAKSYWP
                     NDFGLYNMAGNVAEWTSTAYEDDANAFIHDKQPDFRYDAKTDDPNVMKRKVIRGGSWK
                     DIAYFLQCGTKSYEYQDSAKSYIGFRCLLPFMGRSMNDFGN"
     gene            9039..9884
                     /gene="gldL"
                     /locus_tag="PLL59_09000"
     CDS             9039..9884
                     /gene="gldL"
                     /locus_tag="PLL59_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HMY23987.1"
                     /translation="MAASYFKTKAGKRVKNLLFGVGAAVVIIGAWAKILHIADPKIAG
                     IPFYSYMLTIGLLTEAVIFFISGFIPPEPDYYWEKVYPGLDIYDENWEKNSEKFGHVP
                     LNAPAGGNSLSGQMDKMLEAAKIDQSLVTKFGDNLKGFSDQVSKLSSVADASVSSNEF
                     AGAAKAAAASLNGVKDSFSSAAEGMSKLAAASGDASQYHAQVQSATKNLAALNAVYEM
                     ELQDTNTHLKTMNKFIGNLSSAMTSLEGSIADTEKFKSQMGGLAGNLEKLNGIYGNML
                     SAMRS"
     gene            9937..>10798
                     /locus_tag="PLL59_09005"
     CDS             9937..>10798
                     /locus_tag="PLL59_09005"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23988.1"
                     /translation="MAIPKENRQQMINMMYLVLTAMLALNVSAEILNAFKTINKSLEN
                     SKLTADTNLKTTYNAFAAKKAKEPSNVAVVANEEKTRKAQEITDKLLGYITKLKQEVV
                     TRAGGPDNEDPNGIMMRKDDMDVSTGFLVEGTSGKNGEGYKLKTEIDNTLKELEGLIE
                     PADLQMVKERMSLSTVPNEKNADWVREEFYQMPAIASYSMLTKLENDVKNTQGTLASY
                     FFSKIGASQELTQDQIVFDKFSAQITSPSAYILQGETFEASVSLAATSSKSAGTVTVN
                     VNGQNVPALLD"
BASE COUNT         3709 a         1773 c         1839 g         3477 t
ORIGIN      
        1 aaataaaacc gaatgttccg ctactttcca aaattgaatt gtatgaaaca ctgacctcag
       61 gcgttgtata tgaaggcaaa taaataagct tgttctagca gaaaaattta tagataaaag
      121 ataaaaatct tgcattctgc aagtgaatgt tgtattattg agtatgcaga ttcagcacat
      181 atctttgttg ctcaatattc cactattatt aatcggattt tttctgctca atagaggcgc
      241 aggcatgctg gttgacggtg catcttctat agcacgtcgt ttgcattttc cagaaaccat
      301 gattggcttt acgatagtag catttggcac atctttgccc aaattattta cagttttatt
      361 agcagcttat aatggttatt ctgatttagt tattggaaca atcattggaa gttgcatctt
      421 taatttaatc ggcatattaa gtataacagg attttttagg cctattcgct ccaataaata
      481 tactgcatca ataggactct tgtttttaat agtggcaata gcattgtttt ttctcgtggc
      541 taataaacaa cttatgcctt tagatgcgcc tgcgccaact ttattaatta caagaacaga
      601 agcaattgtc ctattgtctt tttttgtaat ttttatgttg tatggatatt ttaacgtgca
      661 aaggcatcat aaaatatggt tcgacgagat agcacaaaca catgatttaa cttattatgc
      721 atggtggatt tctttgctac tcattcttat cggaattgta ggactagtag ctggtggcgt
      781 tttagtggtc aataatctta tagatatttc tcggaaattt gatttatcac aacggttttt
      841 aggacaattt gtattgtcat ttggcggctc tgcggtgatg ttgtattggc tgatgataac
      901 acaccaaaac aaatcgaaat ttgaactcag taatcttgtt ggtcaaaatg tgctcaatat
      961 agtaggtatg cttggcgttg ctgcctgcat tcagcctatt gtatataatc caattttcaa
     1021 cctcgatatt tatatattaa tagcaatagt ttttattgtc agtggcttat tatggatggg
     1081 aaaaaagctg acacttaata tatggaaatg caggctttta tttctgttat tgcttattta
     1141 tatcgcgtat ttgtttatgc gttagctatt cttttacctt tctgctcaca tgataaacgc
     1201 ctctgccttc agctactaaa atattatcat tgacagcaaa aacttgcata ttcgtcgtga
     1261 taatcttatt accacttctt acaatatttg cttcgatgcg caagtccgta ttgcgtgccg
     1321 aacgaatata atcgacgcgc atatcaacag ttactatttt atcttcgaca ctagttaatg
     1381 tggtcatacc agcaacgcca ccaacggcat ccattgctgc cgaaataaca ccaccatgta
     1441 tagcttgtat tctaatgtcg ccaactaatt cttgcttgaa ttgtattgta gctttcgcaa
     1501 atccagcttc aatatgaact aattctagtc caatatattt attaaacgga ataatttcgt
     1561 tgaatacttt tttataaaaa tcgatattgc ctttcatgtt aaatagtatt ttaattttca
     1621 ataaataaat gtgcacttat gtgatcgcag aataatttcc ctttatccgt taaacgataa
     1681 atgccattgt tttcttctat ccaatttttt gttttaaata atttaatttc tctcagaaaa
     1741 tgctgcacat atttttcctc aaaatcatgt tgtaatttcc gaatatctac accaaatata
     1801 gtgcgtattg atgtcataat atattcgttg tatttgtcgg tttccgtcaa taattcacta
     1861 tcaaaatgcg cttcattcgt tttgattttt ttcaggtaaa tagcattgtt tgcaatgttc
     1921 caatatcttt tttgtccatc aaatgaatgt gctgatggtc caatacctaa gtagggaatg
     1981 cctttccaat aatttgtatt gtgttgtgca aaatgccctt cttttccaaa atttgatatc
     2041 tcataatgaa tataatgatg cgcacgcatt tgtgcagtga gtatgtcgaa ttgtgctgtc
     2101 attgcttcgt cagttggcgc ttcttttttt cgttttttaa taagtgaagc taattccgtt
     2161 ttctcttcaa cagtcaatgc ataacaagac acatgaggca cttgcaggtc gattactgtt
     2221 tggatatttt gtaagcattg tgttgcattt aaagttggtg ttccataaat taaatcgaca
     2281 gataaattgt ggaagcctac atgctgcgca tgctcgacac aagcttttgc ttgagcagcg
     2341 ttgtgtgcac gatgcatata ctgcaaatct gcatcgacaa acgattgaat tccgatgcta
     2401 aatctattta ttggcgtctc tttttttaat gctaaaaggt attcgagcgt taaatcatca
     2461 ggattggctt ctaatgttat ttccgcattg atttgtacat caaaatattt atgtatttgt
     2521 gctagcaatc tatttatatc acctgctgat aatatactgg gtgtgcctcc gccaaaataa
     2581 atagtgttta tgttcgtttt ttctataaaa aaatcttgct gacgggctat ttctaatagc
     2641 attgcctgta aaacatcttc tttatacttc aataaagttg aaaaatgaaa attgcaatag
     2701 ctacatgctt gcttacaaaa cggaatatgc aaataaattc cagccatcag cttacaaggt
     2761 aataacgaat tggtaaatat tgataacttt gacaaagaag atacatcaca tttgcatttt
     2821 tattgtttac aaatagcact ttttctgttt ttcgcacaca gcgtaacagc tcaaaatatt
     2881 cgtttgttta ttcataaaca aaatgaacaa tttaaaatca aagagaaata cacatttaaa
     2941 aatgaaaaag aattacatca gtatctaagt gcaacgcaac aaaaattaat taaacaaggt
     3001 tatgtggagg catcttttga tagtacattt tcaaggaatg attctgtttt cactttcttg
     3061 cattttggca ataaagtgat aataaataat ccatctgttg tcaacaaaaa acgaaaatca
     3121 aaaaatcata cagagacaag tattttcgat atagataaga ataaagaaaa catcttggag
     3181 cagttggaaa acaatggcta tccttttgct aaaatcagta cagatagcct tgtacttagc
     3241 gattctacct tgcattttaa atttaatatt gacaaaggta aattaatcac aattgatagc
     3301 tttgtcaacc atggaacttc taaaatcacg aagggtttta tacagaatta tattttgata
     3361 aaaagcaaac aaccttatcc tgaatctcgc atacaacaga tagataagtt attgcaaaag
     3421 ttaccttacg ctacattgcg acagcctaca aacgtgctct ttcgcgataa taaagctgat
     3481 gtacatttgt acttagataa gcgaaaagta aattcttttg atttcctaat cggttttttg
     3541 ccgggcagta atcgtggaaa aatattgata acaggtgagg tgcgcataca tttgcaaaat
     3601 tcttttaaac gtggcgaaga aatttatata gaatggcgaa aactacaacg ccaatcgcag
     3661 ctgcttgatg tgcgatttaa ttatccttat ttattaaatg caccaatcgg tgttaatttc
     3721 actttctatc ttgaaaaacg agattcctct tcgctagatt tgacctggca actcggcttg
     3781 ccatacatta ctaaatcaaa taattacatc aaaggttttt acaaatattt tcaaaccatt
     3841 gtgctggctg ctgatactgc ttttgctaag ctcaataaaa aattgcccaa caatttagat
     3901 gcaacgtatc atcaatatgg cgtgcaagcg tattacgaaa atttagatta tttatttaat
     3961 cccaaaaaag gttttgaatt aaagttgaca ggctccatcg gtacaaaaaa aataaaacct
     4021 aaccaacaga ttaccacatt acaagatgcc tttaatttta actatgaaag tttgtatgat
     4081 agtattaaac gcagaacgat aaaaggcgat ttattttgga cgggaaatta ttttttacca
     4141 cttggtaaaa agcaaagaca tgtactaaaa tttggcatcg acggtggcgc agtttttaat
     4201 caaaatttgt tgcgtaatga actattgcgt atcggcggta gccgttcatt gcgtggtttc
     4261 gatgagcaat cgattttggc aagtacttat aatatgatga gcttagaata cagaatgatt
     4321 atgatgcgaa attcttattt ctttctattt attgatgcag catatgtaca tcgcaagttt
     4381 aattcaacta cttttcaaga ttttccattc ggatttggaa caggtgtaac ttttgccaca
     4441 aaaattggta tttttgggct cacttatgcg ctcggtcaac aaaaggacaa acctatcgat
     4501 tttagaagtt caaaactaca ttttggctat gtggcaacct tctaagttag tgcttttatt
     4561 tattggttta tttttgttat tcagctctac ttttgcacaa gcaacagcaa ctaacataaa
     4621 tccatttttt agagattcta tattgaaaca caaagtggat acagcacgtt ggtcaaatgt
     4681 ggaaagtgca gtaaatcgat acaataaatc agctacaaac ataagacttc aaaagcccaa
     4741 agttgaaaca aatagcttgt tgtatttata tatgaccgca ggcattgtga tactattaat
     4801 catcatgcgt cttatttttg atgatttttc cacttcatta ctcgaaggaa tgctaagtat
     4861 gaagaagttt tttgtttttt ataaaagtaa aaaatatgac tctctaattg ctataatatt
     4921 agtgtatgta tttaatcttg tcttactttc attgatttca tttatcgctt ggtcgtttac
     4981 gactaaacac ggtttcaaaa ccttcaatat gtcagtgttt atggatatta gcgtgttaat
     5041 gattgttttt tttacgctta aaaatgcgat tgaatatttg tttaatttta taatagatac
     5101 acaggatact ttcaaggcat ttttcctcca aaatttattt acagagttct tgttatgtgt
     5161 ggtcttatta gtgttttcat tgatttatat ttataatagt tattttagcc tcaactggat
     5221 gttgggtttg ttttttgtaa ttcttaccat atatctcgtt ttcaacacta tacgaagtta
     5281 tcaactgata agtgttacac ggctgcctta caaactgcac ttttttttgt atatttgcac
     5341 attcaaatta attccgatac tcttattagt aaagtatttt ttgaataata tcgtttaaaa
     5401 atggcataga aatggctaaa aaattggcag caaaacaaac ttcggtaaag aaaacgtcta
     5461 ctgcaaaaac aactaaaaaa cctgcagtaa aatcaagtgt ctccaaagcg acaaaaaaag
     5521 aaaccaaacc tgctaaaaaa gtagaaaaac ctgtagcaaa accgactaag gcaatagcgg
     5581 caaagaaaac gtcgaaagtt gcagcaccta agatagtagt agaaaaacca gtagcagtag
     5641 ccactaaaga gaaagacacg aaattagata aaaaagtaat tattgaagca aaacctttgc
     5701 cagcaatagt taagccaaat actctaaaac cagtgaaatc aattctgata tcacaaccaa
     5761 aaccagaatt aggaaaatca ccatatatag acttagcaca acattttaaa atcaaattag
     5821 attggcgtcc atttacgcat gtagaaggca tcaaagcact tgattttaga aaacagaaaa
     5881 tatatattga acaatatgct tgcgtcattt ttacaagcag aattgcaatt gaacaatatt
     5941 tcagaatttg cgaagaacta cgtataaaaa taagtgaaga aaataaatat ttctgcatca
     6001 gtgaagccat agcattatat ctccaaaaat tcattcaata tcgcaaacgt aaagtgtttt
     6061 ttggtgatgg taaattgcca aaactcatgg aagtaatctt gaaacataaa gaaaatggta
     6121 aatttttatt gccctgctca gatgtgcgcg ataaagcatt agcagaagca ttaaataaat
     6181 acaatattga atacagcgaa gcatttattt ataaaacagt agccagcgat ttgtcagatt
     6241 taagcgatgt aaaatatgat atgttagtgt tcttcaatcc ttctgctatc gactcattgt
     6301 acactaattt tcctaagttt agtcaagatg aaacacgctt agcaatattt ggcaatacta
     6361 ctgctgacgc agtaaaagca cacaagctaa acatcaatgt ttttgcacct acaccagaaa
     6421 atccttcatt agtagatgct atcaaaaaat acattacgga agcgaataaa aaacgataag
     6481 attcgtttgt tgaactgcag aagataatat ttaattgttc acacaaaata ttttagagca
     6541 ttatcaaaat tcttttatat ttttacgata tgccattacg atttaatcaa ttgattttgc
     6601 tattgtgttg tactatttca tctagtattt tcgcacaaat acaaccggca acatcacatt
     6661 atatgttcca gccgcaagta gctaatcctg cattttatgg cacaaaaaat ggcattaatt
     6721 ttggcgctaa ttataggcat caatgggcgg cactaaatgg tcaaccgcgt actatcaatg
     6781 catttgcaga tgctagtata ccagctgcac acggtggcgt tggtataaac ttgtcgaatg
     6841 atatgctcgg cgcatataat attttaaata taaatgcagg atacacttat atacaagcat
     6901 tacccaaagg cataaaatta aatattggat taaatattgg tgccactttt tccaaattag
     6961 atggtgctaa actaacaacg ccacaaggta ttccaggcga tttgaatgat gatatattat
     7021 ctaatcaagt acaaaaaagt acgcgaccaa gcataacaat cggcttagca ttaacacata
     7081 aatttttaga attcggaata gcatatacaa acgctattca atcaaaagac aaattcaaag
     7141 gcgaaaataa aacagaagca cttcgatatg gtgcaacttt gcaaacctat ataagcagta
     7201 aaatcaaaat caaagcagat ttctttattc gaccttctat attgttagca acagatttta
     7261 aagagctaca aacagaaata agtgttttag gtggctataa gaactatata acactcggat
     7321 ttaacgtaag aggatataat aaaaaagctt ttgagtcgtt atctccaata atcggagtag
     7381 gtccattgaa aaatatttgc atatattata gctatgatgt atctttaagc catctaaaat
     7441 ccgtaaataa aggttcacat gaaatttcat tgaattattt attgccgaat aataaaattt
     7501 ataaaaaccc aaaaatgata aataacccaa ggtttttgta aaaagattat taatatttta
     7561 gataaaatat taaacatgat gtacaagtaa acaattaaat tgttattttt acgtctattt
     7621 taggataaaa acgtaaacaa ctaagatgaa taagttaaaa ttctcgttta ttgcagtgat
     7681 agctgtattg ttagccagtt gcaaaggtgg taatggtaca gatggtcaat taatcggatc
     7741 gcaggatcgt ccaaagtggg acaataacct gcttccttat ggaatgattt atgttccatc
     7801 aggtgtattc catacaggtc catctgacca agatgtgaat tacgcattta acaccaaaat
     7861 gaaacaattg tctattgtag gtttctacat ggatgaaaca gaaattacaa ataacgaata
     7921 ccgtcaattt gttgagtatg ttcgcgattc aatcgctcac caaatgctcg gtggcgacca
     7981 tttaaaagaa ggtgataatg gcaaacaagc gatcaattgg gatatgccta ttgatatgga
     8041 ttggagtaat ggctcaagtg ttacagaaga aggcgcacaa ttagatccaa tgttctattc
     8101 agaagctgat cgcttaaatg gtagaaaaga agtagatcct cgtaaattag aatatgaata
     8161 tacttggttc aaatggaaag aagcagcaca acaacgtcct acagataaag atgtaaaacc
     8221 aagaagtgca tttatggaga aaaagaaaat ttcaatctat cctgatgaaa tggtatggat
     8281 tcgcgatttc tcttattcat acaatgaacc aatgacacgt tcttacttta cacatccagc
     8341 ttatgacgat tatccagttg tgggcgtcac ttgggatcaa ggtaatgcat tctgcgcttg
     8401 gagaacatta atgtggaaca actacagaat gtcacaaaaa gaagcacctt tagacgaatt
     8461 ccgtttacct acagaatatg aatgggaata tgcagctcgt ggtggcagaa atttatcacc
     8521 ttatccttgg ggtggtcctt acgttagaaa tacaaaaggt tgcttattag caaactttaa
     8581 accaggcaga ggtaattatc cagaagatgg tggcttctat actgtaaatg ctaaatctta
     8641 ctggccgaat gacttcggtt tatataatat ggctggtaat gttgcagaat ggacatctac
     8701 tgcttatgaa gatgatgcta atgcatttat ccatgataaa caacctgact tcagatatga
     8761 tgctaaaaca gatgatccaa atgtcatgaa acgtaaggta atcagaggtg gctcatggaa
     8821 agatattgcg tacttcttac aatgtggtac aaaatcttat gaataccaag attcagcaaa
     8881 atcttacatt ggtttcagat gtttattgcc tttcatgggc agatcaatga atgattttgg
     8941 caattaatca tactatttta acttataata cgtttaaaag aaaataaaaa tatttaacca
     9001 aataattttt aaaactaacc aaataaaaaa aacacattat ggcagcaagt tattttaaaa
     9061 ccaaagcagg caaaagagtt aaaaacttac ttttcggtgt tggtgcagca gtagtaatta
     9121 ttggtgcgtg ggcaaaaatc ttacacatcg cagatccaaa aattgcaggt attccttttt
     9181 attcatatat gttaacaatc ggtttgttaa cagaagcagt tatcttcttt atatctggtt
     9241 ttattccgcc agagccagat tattactggg aaaaagtata tcctggttta gatatctatg
     9301 atgaaaattg ggaaaaaaat tcagaaaaat ttggtcatgt tcctttaaac gcaccagctg
     9361 gtggcaatag cctttctggt caaatggaca aaatgttaga agcagcaaaa atcgaccaaa
     9421 gtttagtgac taagtttggc gataacttaa aaggtttctc tgatcaagta agcaaattat
     9481 caagcgtagc tgatgcttct gtgtcttcta atgaatttgc aggtgcagct aaagcagcag
     9541 cagcatcttt aaatggtgtt aaagattctt tctcttctgc agcagaaggt atgagcaaat
     9601 tagcagcagc ttctggcgat gcttctcaat atcatgcaca agttcaaagc gcaactaaaa
     9661 acttggcagc attaaacgct gtttatgaaa tggaattaca agacacaaac acacacttga
     9721 aaacaatgaa taaattcatc ggtaatcttt cttcagctat gacaagctta gaaggttcta
     9781 tcgctgatac agaaaaattc aaatctcaaa tgggtggctt agcaggcaac ttagaaaaat
     9841 taaatggtat ctacggaaac atgttatctg ctatgcgttc ataatcactt ttttcttaac
     9901 tattattaat taactaaaag agtaaaaaag taaattatgg caattcctaa ggagaacagg
     9961 cagcagatga tcaatatgat gtatctggtg cttacagcaa tgctagcatt aaacgtttcc
    10021 gcggaaatct taaatgcttt taaaacaatc aataagagtt tagaaaactc taaacttaca
    10081 gcggatacta atttgaaaac tacatataat gcgttcgcag ctaaaaaggc aaaagaacca
    10141 tctaacgtag ctgtagttgc aaatgaagaa aaaactagaa aagcacaaga aatcacggat
    10201 aaattattag gttatatcac taagttaaaa caagaagtag taactagagc tggtggtcct
    10261 gataatgaag atccgaatgg tatcatgatg cgtaaagatg atatggacgt atctactggc
    10321 ttcttagtag aaggtacgag tggtaaaaat ggtgaaggtt acaaattaaa aacagaaatt
    10381 gataacaccc ttaaagaatt agagggctta atcgagccag ctgatctgca aatggtaaaa
    10441 gaaagaatgt ctttatcgac tgtaccaaac gaaaaaaatg cggattgggt aagagaagaa
    10501 ttttatcaaa tgccagcaat cgcttcttat tctatgttga cgaaattaga aaatgacgtt
    10561 aaaaatacac aaggtacttt agcttcttat ttcttcagca aaattggtgc atctcaagag
    10621 ttaacgcaag accagatcgt attcgataag ttctctgctc aaatcacttc accatctgca
    10681 tacatcttgc aaggagaaac atttgaagca agcgtttctt tagcggcaac atcatctaaa
    10741 agtgctggta cagttactgt aaatgtaaac ggtcaaaacg taccagcact tttagatg
//
LOCUS       DAOQQV010000085        10753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2216_length_10753_cov_6.586839, whole genome shotgun sequence.
ACCESSION   DAOQQV010000085 DAOQQV010000000
VERSION     DAOQQV010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_10753_cov_6.586839"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLL59_09010"
     CDS             complement(<1..348)
                     /locus_tag="PLL59_09010"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HMY23989.1"
                     /translation="MKIYTKKGDTGTTALYGGLRLSKADLRIEAYGNVDELNSFIGLA
                     ATYMEEKEYSDLIQDIQSRLFDIGTHLAAEPGKKNLILPEIKEEYIVVLEQYIDKMNE
                     HLPELKFFILPGGN"
     gene            complement(464..1243)
                     /locus_tag="PLL59_09015"
     CDS             complement(464..1243)
                     /locus_tag="PLL59_09015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMY23990.1"
                     /translation="MKKQTLSIMVLAIMAIMTACKSKNDFSGNTPKKGIDSVSYAIGV
                     QIGEDLKRNGIDSSFNVEVLARGIYEAINNKKLLFGGDTLTDVLMKHFNPQMFAMVEE
                     NNKKAKKFFEENAKRPGVKTTASGLQYEVITEGTGPKPKATDVVKVDYVGTLLDGKEF
                     DSSIKRGEAATFPLNGVIPGWTEGLQLMNVGSKYKLYVPGKLAFGPQGQQQAGIGPNE
                     TLVFEVELKGIEPPQAAGQGPDNAALQKMMEEALQKQAAGK"
     gene            complement(1275..2063)
                     /locus_tag="PLL59_09020"
     CDS             complement(1275..2063)
                     /locus_tag="PLL59_09020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMY23991.1"
                     /translation="MNTKFFIAAFALLTMTTMAEAKTKCCKKKVKCKANEVSSKIIDS
                     VSLSNKIDTISYFLGMQIGADMERNGASDINPDALKQGLSDALKKSVSKVDMQTGMVY
                     AQSYFMEKQAIKKAAEEAVAKKFFDDLAANPNIKTTASGLRYEVVKDTVGEKPLATDK
                     VTVHYTGTLVNGKVFDSSEGGEPVTFPLNQVIKGWTEGVQLMSIGAKYKFYIPGNLAY
                     GEQGVPQAGIGPNETLIFDVELLKIEKPAPQLQQAPSNIKIEQQ"
     gene            complement(2139..3263)
                     /locus_tag="PLL59_09025"
     CDS             complement(2139..3263)
                     /locus_tag="PLL59_09025"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMY23992.1"
                     /translation="MQKKITPLKLITILIVLMLSVIVLYYNETLRVPSIFDGKFYTSA
                     LIVPATKSEPLPEKSIALPETNLWLDSAMAIQFPYPDFFLEKTSTTSFVVVRNDSIIF
                     KRYLNGVKEGENTQLFSVTKVFVTALLGMAIQDKYIKNPDEPVTHFFKNLPSAQFQHL
                     TLRHLMQMQSGLNYDEYGKIFQTLQYYYEKNLTRAIYGATFDTVPGFLFKYKSIDTQI
                     LGECIKNALGNKKILDYFHERLWNQLGMQDTAYWALDSRLMRNPKYYGGLNMSARDLA
                     KFGVMILHDGKFKRKQLLPKDWFNYCDDTLHRCKEEDKYCMGWYYSVDDDNSDVYYAA
                     GFNGQIMLINETKNVIIIRLGKDRGGVNWYPIMKKLSESV"
     gene            complement(3281..4084)
                     /locus_tag="PLL59_09030"
     CDS             complement(3281..4084)
                     /locus_tag="PLL59_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY23993.1"
                     /translation="MSSAQGVLDSTAQDTLLLQAVDDSSLMKAAPISNIEATTLNAVN
                     NLTITKKKKKYSIDKVRILFGVNYSFYQALMTERDIFQGVYYPVKDSAPVWTERDTKN
                     NLAKQYKSSNFQFSIQGNVWKGLFVGMHYQFFSVKQYKKDENRGNLLSKVNTMFFIAS
                     AQIGYVFEFLKDKTLQLHPSIRIGGFTADDYYDSGKGKKLFFGTDIQLRYLIKRKAGF
                     SLGFDYDFLHYKQKDYTDIFHRKTYQKTSFSNFHLNAGVFVNVSINTKK"
     gene            complement(4182..>10753)
                     /locus_tag="PLL59_09035"
     CDS             complement(4182..>10753)
                     /locus_tag="PLL59_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY23994.1"
                     /translation="GNDLLCNVSSVSTYDIGTAATVAANVGSVNNISKTGTNINGTNC
                     FDPNPTWGGVCAASFNNDNDYGVWYKLPAATNRKDILIKGVTTGSGDLTDLQYALYEA
                     SAPTNCLNPGATLISGSYYGSLAWNSVAGFCASADEDHIYTCLDPAKDYYLMVDGNDQ
                     AISCGTGNFTLNTYYPREGGVLPCSAEPLPGVFPTWTAGTHKINLAANFCGTPGGHAG
                     APTAPWTYEKPVWWKFVAPPSGSVEIRMKSDSTNLGDEIRPRLWVFKENTPGTCSNTL
                     LNTTDLDQKDPDDIIGIGDPDLLTEILDVRCLTPGQTYYLVTDGFNTIAEVCGAGTKV
                     GFFSLEIKDLGQAKRDNDNICGATPGRVAGNPYYFTPTADWKTVNKEAELTKSDQDNY
                     CFVIENEPDASDPTWGGAWSTSNNARGGWYSFRAPPSGKVEIELENKSFTADQIDAQI
                     AVYRIKNGNTCDQVQTYTPASGTSSLLEFYGSSNDTYAGVLADEDLTVSCLMPDSLYY
                     IYFEGVNSNTLGVNDLATGQFHLTVRSYPQDPPAKNDNKCNPIYLGKPANATLGSGNP
                     GYINASSSANFNRNLSGSTGTPPTLGGNGVTLVKATNNHGATIPGESTLSCSPNPGCI
                     DGLSEDYDRNLYPFNNFCATAVGDSVPSTWGGFAGAGDKQPRKTVWFMFQAPSDLDGD
                     GNEAVLIELNQETIAINAHKDGMDLRVAVYESSDNTCNGNFYELNSDYDQGNYDEDVK
                     VTCLEPDRYYWIMVDGSSFFSGSDEGYFGMKISRVAPDPRPSNDYICNAHTFSNSFFT
                     GAAVGRTRDTNICARITRTNIDPTPENDPSGFDLDHTVWYKFTAPTTGVPPTGAFAVQ
                     VDVNGWGPGLFGYSDKMDPQIAIFESQDGTCNWGTNGTNMVEIASEYDILPFTESMKA
                     YCIIPGKTYYVMVDGSALNSQGYFDISINDIAPITLPTNDDLVNATALTFPSNIGAGN
                     TTTSALAHNYCTDIEPGELDAWTLYDIDNTVWWKFQVPDSGAYTNQHVDLQIRLRSDP
                     GNTKNDKINLLGAVYHQIGGTGGSPGTYPNINENIGTGTNIAIFDEDINLTCLKPGDW
                     YFLQIDGYSGPFNIFRAQGQGWYDVQLEVTGLTPNQGNDSVCGAIVIPVNNIAQPTSG
                     APYNNICKTTQANETLPWINADSKTVWFKFIPPASGNINIQVQSEASDDIDAQIAVYQ
                     SLIPVASSACPPVDQLILAGKDYDALAWGEDMNIKCLDNQYWHYLQVNSANVGLVREG
                     KFKVKIQDIGGTTNPPYNDNICNARDFGNITNANGTGAFNIVGDSNKCATIQLNEPST
                     ISNPTSDIQKSVWYKFTAPTSGRAKITLHDQDGFLSGIDPEMKLYEGTLSGCPSASPT
                     FTNFTVLESAYNPLPNFPTATTGDEVIEYECFIPGQIYYIQVDGTTVGGPQGFFDIKI
                     EDMIPNYPSSPKKPVNDEPTLATTLTVNQQQCPVPIGITSEGDELQGNGTWLTGNYKK
                     PTITKKDLIGTCNTLDNCGDIWYKFTMPNDDCMLGSVVAIQGYSDRAPNVGDQYYNDL
                     KIIAYRGTPGSLVPIECGKVDGNFATNDYFHFEVTGTPGETIYLQVFDQNYNDTDNDD
                     EEDFFICVGKRFGTDKCQSLSNVPFMEYSKDYCWNIEGASGETPASAYGETNSATNPT
                     ENSAYFKFKMTSNPCDSLNFTIWQPAPPNLFLQGGGTNLNQIVSLSVYREDNALCDGT
                     IDGALISKTYICSSPTAILDNTLRFNVGLSNLDTNKTYVVQIDGQGQAESGYINDGFI
                     RIDTFQRCVNVQAVTYDDTSTGYWKCVDGWRYYHDRNNVIIFALYPNGNNFDGIAKIR
                     YKSNYDEASICSIPMAEYSMRRIWDFHMTSGSINPLNPVKVRFYYIATEKQAIINAAL
                     AFQGSCGGYYEPFEWFKTANGVQYIPTNPAQINPGIINAAYSETACLGSSLFGSPNGC
                     NTLTALDNQYACNGTLYVELGAITGFSGGTGAAGVGPWQNASPLPVELISFTGYNDGA
                     KNVLNWSTASERNTLKFEVERASSNIGQFTYIGELPAAGNSSQPLAYTLDDNRPNIGE
                     NYYRLKMIDRDGSFKYSHTILIRNEEVITSNAINGIYPNPTSHLINIDYQSVNSGNAV
                     LKIVNVIGQEISSTVQAIVKGKQIIQIDVSSLADGVYILNIQDSQNGKVLQSKFVKD"
BASE COUNT         3139 a         2113 c         1818 g         3683 t
ORIGIN      
        1 attgccacct ggcagaataa aaaatttcaa ttctggtaaa tgctcattca ttttgtcgat
       61 atactgttct aagaccacaa tatattcctc tttaatttct ggtaaaataa gatttttttt
      121 gcctggctct gcggccaaat gtgtgccgat gtcaaataat ctgctttgta tatcttgaat
      181 taaatcgctg tattcttttt cttccatata cgttgccgcc aagccaataa acgaatttaa
      241 ttcgtcaaca tttccatagg cttcaatgcg caaatcggcc tttgacaaac gcaatccgcc
      301 atagagtgcc gtggtgcctg tatctccttt tttagtgtat attttcattt ttatattttt
      361 cgatgttaca atgtacaacg tagttttgtc aatttcaatt tatgttttac acaaaaaaag
      421 gctgtcctca cagacagcct ttttttaaaa tttaggatat aaattattta ccagctgctt
      481 gtttttgcaa tgcttcttcc atcatttttt gtaatgctgc gttgtctggt ccttgtcctg
      541 ctgcttgagg tggctcaatg ccttttaatt ctacttcaaa cactaaagtt tcgtttggtc
      601 cgatacctgc ttgttgttgc ccttgagggc cgaaagctaa tttgccaggc acgtacaatt
      661 tgtacttaga gccaacattc attaattgca aaccttctgt ccaacctgga attacaccgt
      721 tgagaggaaa cgttgctgct tcaccgcgtt tgatagaact atcaaattct ttaccatcta
      781 aaagtgttcc aacatagtca actttaacca cgtcagtagc tttcggtttt ggacctgtgc
      841 cttctgtaat tacttcatat tgcaaaccac ttgccgttgt ttttacgcct ggtcttttag
      901 cattttcttc gaagaatttt tttgcttttt tgttgttttc ttccaccatg gcaaacattt
      961 gcggattgaa atgtttcatt aatacatcgg ttaatgtgtc gccaccaaac aataattttt
     1021 tattattgat tgcttcataa attccacgtg ctaatacttc tacattaaaa gaactatcaa
     1081 taccatttcg ttttaagtct tcaccgattt gaacaccaat tgcataagaa acagagtcga
     1141 ttcctttttt aggtgtattt cctgaaaaat cattttttga tttacaagcg gtcataatgg
     1201 ccatgattgc aagcaccatt attgataatg tttgtttttt catttttaaa attattattg
     1261 aaattgattt ataattattg ttgttcgatt ttgatattag aaggtgcttg ttgcaattgt
     1321 ggagctggtt tttctatttt taataattcc acatcaaata tcaaagtttc gttgggtcca
     1381 atacctgctt gtggcacacc ttgttcgcca taagctaaat tgccaggaat atagaattta
     1441 tatttagcac caatcgacat taattgcacg ccttctgtcc agcctttgat gacttgattt
     1501 aaagggaaag taaccggttc gccaccttct gagctatcaa atacttttcc gtttacaagt
     1561 gtgcctgtgt agtgtacggt cactttgtcg gtagccaagg gtttttcgcc tactgtatct
     1621 tttacaactt catagcgcaa accgcttgca gtagttttta tgtttgggtt cgctgctaaa
     1681 tcatcaaaga attttttagc cacagcttct tctgctgctt ttttgattgc ttgtttttcc
     1741 ataaaataag attgtgcata caccatacca gtttgcatat ctacttttga tactgatttt
     1801 tttaaagcat cggacaagcc ttgtttcagt gcatctggat tgatgtcgct agctccatta
     1861 cgctccatat cagcaccaat ttgcatacct aaaaaataag aaatagtatc gattttattt
     1921 gacaagctaa cagaatctat gatttttgaa cttacttcat tagccttaca ttttactttt
     1981 ttcttacagc atttagtttt tgcctcagcc atagttgtca tcgttaacaa cgcgaaagcg
     2041 gcaataaaaa atttagtgtt catttttttt aaattagaac tgcaaaaata aggtatttgc
     2101 ctttataaat gtatttatta actattttta atgtggtttt aaactgattc ggataatttc
     2161 ttcatgattg gataccaatt tacgcctcca cgatctttcc ctaaacgtat aataataaca
     2221 ttttttgttt cattgatgag cattatttga ccgttgaaac ctgcggcata atagacatca
     2281 ctattatcat catcaacgga ataataccaa cccatgcagt atttatcttc ttctttacaa
     2341 cgatgcaaag tatcatcaca atagttaaac caatctttag gcaataattg tttgcgttta
     2401 aatttgccat cgtgtaatat catgacgcca aattttgcta aatcgcgtgc tgacatattc
     2461 agacctccat agtattttgg attacgcatc aagcgactat ctaatgccca gtatgctgta
     2521 tcttgcatgc ctaattggtt ccataagcgc tcatggaaat agtctagtat ttttttattg
     2581 cctaatgcat ttttaataca ttcacccagt atttgtgtat cgattgattt atacttaaac
     2641 aaaaatccag gtactgtatc aaacgttgct ccatagatag cacgcgtgag atttttctcg
     2701 taataatatt gaagtgtttg aaatattttt ccatactcat cataatttaa accagattgc
     2761 atttgcatta agtggcgtag cgttaagtgt tggaattgtg cgctcggcaa atttttaaaa
     2821 aaatgggtaa ctggttcgtc cggatttttg atatacttat cttgaatggc catacctaac
     2881 aatgcggtta caaacacttt tgttacagaa aacagctgtg tgttctctcc ttcctttacg
     2941 ccattcaaat atcttttaaa aatgatgctg tcatttctta ctacgacaaa tgatgtcgta
     3001 ctggtttttt ctaagaagaa atcaggatat ggaaattgaa ttgccatcgc actgtctaac
     3061 cataagttgg tttctggcaa tgctattgat ttttctggca atggctctga ttttgttgct
     3121 ggcacaatta atgcgcttgt ataaaattta ccatcaaaaa ttgatggcac tcttaaggtt
     3181 tcattatagt ataatacaat tacactcaac attaaaacga taagtatcgt gatgagtttt
     3241 aatggtgtaa tttttttttg cattcgttag tacaatagtt ttactttttt gtattgatgg
     3301 aaacattgac aaacacacct gcattcaaat gaaaattgct aaatgaagtt ttctgatacg
     3361 tttttctatg gaaaatatct gtgtaatctt tttgtttgta gtgtaagaaa tcataatcaa
     3421 aacctaagct gaagccggct tttcttttta ttaagtaacg caattgaata tctgttccaa
     3481 aaaataattt tttcccttta ccactatcat aataatcatc agcagtaaaa ccaccaattc
     3541 tgatggaagg atgtaattgt aatgttttat cttttaagaa ctcaaacaca tatcctattt
     3601 gagcagatgc aataaaaaac atggtattta cttttgacaa tagattacca cgattttcat
     3661 ctttcttata ttgcttcact gaaaaaaatt gataatgcat accgacaaat aatcctttcc
     3721 atacattgcc ttgaatacta aattggaaat tactgctttt gtattgcttt gcaagattgt
     3781 ttttagtatc gcgttccgtc cacactggcg cagagtcttt cactggatag tacacacctt
     3841 gaaaaatatc gcgctctgtc attagtgctt ggtaaaaaga ataattgaca ccaaaaagaa
     3901 ttcgaacttt atcaatcgaa tattttttct ttttttttgt aatagtaaga ttatttactg
     3961 catttagcgt tgttgcttct atatttgata ttggagctgc cttcattagg ctactatcat
     4021 caactgcttg caacaataaa gtgtcttgcg ctgtagaatc taatacacct tgtgcagatg
     4081 acatacaata aatacaacaa aaaattactg atagaaaaaa tctcatagta caaaaataaa
     4141 aaatcccgac taaatgtcgg gatttaatat gcaagtttac tttagtcttt tacaaactta
     4201 gactgcaata cttttccgtt ttgcgaatct tgaatattta agatatatac gccatcagca
     4261 aggctgctca cgtctatctg gataatttgt ttgcctttga caatagcttg tactgtacta
     4321 ctaatttctt gtccaatcac attaacaatt ttcagcactg cattaccact atttacagat
     4381 tgatagtcga tattaatcaa gtggctggtt ggattcggat aaatgccatt aattgcattt
     4441 gatgtaatca cttcttcatt tcgaattaga atagtatgcg aatatttgaa gctaccatct
     4501 ctgtctatca ttttcagtct gtaataattt tcgccaatat tcggtcgatt atcatctaat
     4561 gtataagcca atggctgact gctatttcca gcagcaggca attcgccaat gtaggtaaat
     4621 tgacctatat tactagatgc acgttctacc tcaaatttca gcgtattgcg ttctgatgct
     4681 gttgaccaat tcaacacatt tttagcacca tcattatatc cagtaaatga aattaattcc
     4741 actggcaatg gcgaagcatt ctgccatgga ccaacacctg cggcaccagt tccgccagag
     4801 aagcctgtta ttgcgcctaa ttcgacatat agcgtaccat tacaagcata ctgattatcg
     4861 agtgctgtta aagtattaca accattcgga ctgccaaaca aactacttcc taaacatgcc
     4921 gtttcagaat aagctgcatt aataattcct ggattgattt gtgctggatt agtcggaata
     4981 tattgtacgc catttgctgt tttaaaccac tcgaaaggtt cgtaatatcc accgcacgag
     5041 ccttggaatg ctaaggctgc atttattatc gcttgtttct ctgtggcaat ataatagaac
     5101 cttactttaa ctggatttaa tggatttata gagccacttg tcatatggaa gtcccaaata
     5161 cgacgcatac tatattctgc cattggtata gaacaaattg atgcttcatc ataattagat
     5221 ttgtatctaa tttttgcaat accatcaaag ttattaccat tcggatatag tgcaaagatg
     5281 ataacattat ttctatcgtg ataataacgc cagccatcta cacatttcca atagcctgta
     5341 cttgtatcat catacgttac tgcttgaaca tttacacaac gctggaaggt atctattctg
     5401 atgaatccat cattaatata acctgattct gcttgtcctt gtccatctat ttgtacgacg
     5461 taggtcttat tggtatctaa attactcaag cccacattga accttaatgt attatctaaa
     5521 atagctgttg gtgaactaca gatatacgtt ttagaaatta atgcgccatc aattgtacca
     5581 tcgcataatg cattatcttc acgataaaca cttaaggaga ctatttgatt aagatttgtt
     5641 ccgccacctt gcaagaataa gtttggtggt gctggttgcc aaattgtaaa attcaagcta
     5701 tcgcaaggat tcgaagtcat cttaaattta aagtaagcag agttttctgt cggattggtt
     5761 gcagagtttg tttcaccata agcagatgct ggtgtttcac cagaagcacc ttcaatattc
     5821 cagcagtaat ctttactata ttccataaat ggaacattag ataaactttg acatttgtct
     5881 gtaccaaaac gcttgccaac acagataaag aaatcttctt cgtcatcatt atccgtatca
     5941 ttataatttt ggtcaaatac ttgtaaatag attgtctcgc caggcgtacc tgttacttcg
     6001 aaatggaaat agtcgtttgt agcaaaattg ccatcaactt taccacactc gataggcact
     6061 aaactaccag gtgtacctct ataagcaatt attttcaagt cattataata ttgatcacct
     6121 acgtttggtg ctctgtcgct ataaccttga atagcaacaa cactacctaa catgcaatca
     6181 tcatttggca tagtaaattt ataccagata tctccacaat tatctaatgt gttacaagtg
     6241 ccaatcaaat cttttttcgt gatagttggt tttttataat tccctgtcag ccatgtgcca
     6301 tttccttgta gttcatcacc ttcagaagtt atgcctattg gcactggaca ttgttgttgg
     6361 tttacggtca atgttgtagc aagcgtaggt tcgtcattta ctggtttttt aggtgaagac
     6421 ggatagtttg gtatcatatc ttctattttg atatcaaaga atccttgtgg accgccaacg
     6481 gttgtgccat caacttgaat gtaatatatc tgaccaggaa taaagcattc atattcgatg
     6541 acttcatcac cagttgttgc cgttgggaaa tttggcaatg gattataagc actttctaat
     6601 actgtaaaat tggtgaatgt aggcgatgcc gaaggacaac ccgacagcgt cccttcatac
     6661 aatttcattt ctggatcaat gccactcaag aagccatctt ggtcgtgtaa agtaattttt
     6721 gcgcgaccag atgttggagc cgtaaattta taccacacgg atttttgaat atcagaagtc
     6781 ggattagaaa ttgtacttgg ttcatttaat tgaattgttg cacatttatt gctatcgcca
     6841 acaatattaa atgcgcctgt tccattagca tttgttatat ttccaaaatc tctcgcatta
     6901 caaatattat cattataagg tggattagtt gtgcctccaa tatcttgaat tttcaccttg
     6961 aattttcctt ctctaactaa gcctacattg gcgctattta cttgaaggta atgccaatat
     7021 tgattatcta agcattttat gttcatatcc tcgccccaag ctaaagcatc ataatctttg
     7081 cctgccaata ttaattggtc aacaggtgga cacgctgatg aagctaccgg aattaaagat
     7141 tgatacaccg caatttgtgc atcgatatca tcagatgcct cgctttgcac ttgtatgttt
     7201 atattaccag aagctggtgg aataaactta aaccaaactg tcttgctgtc tgcatttatc
     7261 caaggaagtg tctcattggc ttgtgttgtt ttacaaatat tattataagg cgcacctgat
     7321 gttggttgtg caatattatt tacaggaata acgattgcac cacaaacact atcattacct
     7381 tgattaggcg ttagaccagt tacttctaat tgcacatcat accaaccttg tccttgtgct
     7441 ctgaatatat tgaatggtcc tgaataacca tcaatttgta agaaatacca atcgcctggt
     7501 ttcaaacatg tcaaattaat atcttcatca aaaatggcaa tgtttgtacc tgtaccaata
     7561 ttctcattga tatttggata agttcctggt gagccaccag ttccgcctat ttgatgatag
     7621 acagcaccta acaaattaat tttatcattc ttagtattac caggatcgct acgtaaacgt
     7681 atttgcaaat ctacatgctg atttgtgtaa gcgccagaat ctggcacttg gaatttccac
     7741 catactgtat tatcaatatc atataatgtc caagcgtcca attcgcccgg ctcaatatct
     7801 gtacaataat tatgtgctaa tgcagatgtt gttgtatttc cagcaccaat attacttggg
     7861 aaagtcaatg ctgttgcatt taccaaatcg tcattcgttg gcaaggtaat tggagcgata
     7921 tcattaattg aaatgtcgaa ataaccttgt gaatttaatg cagaaccatc taccattaca
     7981 tagtaagttt tgcctggaat aatacaatat gctttcattg attctgtaaa tggcaagata
     8041 tcgtattcgc ttgcaatttc gaccatattg gtgccattgg ttccccagtt acatgtgcca
     8101 tcttgcgatt caaaaatggc aatttgtgga tccattttat cagaatagcc gaacaaacct
     8161 ggtccccagc catttacatc tacttgcaca gcaaatgctc ccgttggtgg cacacctgta
     8221 gttggtgcag taaatttata ccagactgtg tggtctaaat caaagccact tggatcgttt
     8281 tctggtgttg gatctatatt cgttcgtgta atgcgtgcac atatattagt atcacgtgtg
     8341 cgaccaactg ctgctccagt aaagaaagaa ttactaaaag tatgcgcatt acagatataa
     8401 tcattagatg gtcgtggatc aggcgctact ctacttattt tcataccaaa ataaccttca
     8461 tcggaaccac tgaaaaagct ggagccatct accataatcc aataatatct atcaggctct
     8521 agacaggtta cttttacatc ttcatcataa tttccttggt cgtaatcgct attgagttca
     8581 tagaaatttc cgttacatgt attgtctgat gactcgtaaa cggcgactcg taagtccatt
     8641 ccatctttat gagcattgat tgcaattgtt tcttgattca gttctattaa aacagcttca
     8701 ttgccatcac catctaaatc cgatggtgct tggaacataa accaaactgt tttgcgaggt
     8761 tgcttatcgc ctgcaccagc aaatccaccc caagtagatg gcacggaatc gccaactgca
     8821 gttgcgcaga agttattgaa cggatataaa tttctgtcat agtcttctga caatccatct
     8881 atacaacctg gattaggcga gcaagataaa gttgactcac cgggtattgt tgcaccatga
     8941 ttattcgttg ctttaacaag cgtaacacca ttgccaccga gagtaggagg tgttccagta
     9001 gaaccactca agtttctatt gaagttcgca cttgaagaag cattgatgta gcctggatta
     9061 cctgagccta aagttgcatt cgctggttta cctaaatata ttgggttaca cttattatca
     9121 tttttagcag gtggatcttg tggataagaa cgaacagtca aatgaaattg acccgtagct
     9181 aaatcattta cacctaaggt attactatta acgccttcga agtatatgta atataagcta
     9241 tcaggcatta gacatgaaac cgttaaatct tcatcagcaa gaactccagc atacgtatca
     9301 ttactactac catagaattc taacaatgag ctcgtaccac ttgcaggtgt ataggtttgt
     9361 acttgatcac atgtattgcc atttttgatt ctatatacag caatctgtgc atcaatctgg
     9421 tcagcagtaa acgatttatt ttctaattca atttcaactt ttccagatgg tggagctcta
     9481 aatgaatacc aaccacctct tgcattgtta gaagttgacc aagctccacc ccacgttggg
     9541 tcagatgcat ctggttcatt ttcgatgaca aagcaataat tatcttgatc tgattttgtc
     9601 agttctgctt ctttatttac tgttttccaa tctgcagttg gagtaaaata ataaggattt
     9661 ccagcaactc tgccaggtgt tgctccacaa atgttatcgt tatcgcgctt cgcttgaccg
     9721 agatctttta tttccaaact aaagaaacct actttagtgc cagcaccaca aacctctgcg
     9781 atggtattga atccgtctgt tactaaataa taggtttgtc caggagttaa acaacgaacg
     9841 tcgagtattt ctgtcaacaa atcagggtcg ccaatgccaa tgatatcgtc agggtctttt
     9901 tggtctaaat cggttgtatt tagtaatgta ttgctacaag tacctggtgt gttttctttg
     9961 aatacccata gtcttggtct aatctcgtca cctaaattcg ttgaatcgga tttcatacga
    10021 atttcaacag aaccagacgg cggtgcaaca aatttccacc atactggttt ctcgtaggtc
    10081 cacggtgctg taggtgctcc agcatgccca ccaggtgtgc cacagaaatt tgctgcaagg
    10141 tttattttat gtgttcctgc tgtccaagtt ggaaaaacgc caggcaatgg ctctgcagaa
    10201 catggtaaga cgccaccttc tctaggataa taagtattta gcgtaaaatt acctgtaccg
    10261 caagaaattg cttggtcatt accatctacc ataaggtaat aatcttttgc aggatctaaa
    10321 caagtgtata tatgatcctc atctgctgaa gcacaaaaac cagcgacaga attccaagct
    10381 aaagaaccat agtaagagcc tgaaattaat gtagctcctg gattcaagca atttgttggt
    10441 gctgatgctt catataaagc atattgtaag tcggttaaat cgccactgcc tgtcgttacg
    10501 cctttaatta aaatatcttt acgatttgtt gcagcaggca atttgtacca aacgccataa
    10561 tcgttatcat tattaaatga ggcagcacaa acaccacccc aagttgggtt aggatcgaag
    10621 cagtttgtac cgttaatatt tgttccagtt ttagatatat tatttactga acctacattt
    10681 gcagcaacag tggcagccgt tccaatatca taagtagaaa cgctggatac attacagagt
    10741 aagtcgttgc cag
//
LOCUS       DAOQQV010000086        10719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2227_length_10719_cov_7.019974, whole genome shotgun sequence.
ACCESSION   DAOQQV010000086 DAOQQV010000000
VERSION     DAOQQV010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2227_length_10719_cov_7.019974"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLL59_09040"
     CDS             complement(<1..754)
                     /locus_tag="PLL59_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMY23995.1"
                     /translation="MPYKIKQLYEGSLHFLHFMFYSYFYGMTDIDFNTNEDGMRLAID
                     ELKSKLQKIYLGGGQKKIDAEHAKNKLTARERIDYLVDKNSEYIEIGALVGDGMYAEQ
                     GGCPSGGVVAVIGYVAGRQCMIVANDATVKAGAWFPITGKKNLRAQEIAIENRLPVIY
                     LVDSAGVYLPMQDEIFPDKENFGRVFRNNAKLSSMGVPQIAAIMGSCVAGGAYLPIMC
                     DESIIVDGTGSIFLAGPYLVKAAIGENVDKETL"
     gene            830..927
                     /gene="ffs"
                     /locus_tag="PLL59_09045"
     ncRNA           830..927
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLL59_09045"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            996..1250
                     /locus_tag="PLL59_09050"
     CDS             996..1250
                     /locus_tag="PLL59_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HMY23996.1"
                     /translation="MPIPQPNNPLHGITLEKMLRYLLAYYTWEGLYKQVPANCFKNNP
                     SIKSSLTFLRKTPWARQRLETIYIYITRDGVNEEWFAFEE"
     gene            1352..2980
                     /locus_tag="PLL59_09055"
     CDS             1352..2980
                     /locus_tag="PLL59_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMY23997.1"
                     /translation="MITVNNLSLQYGKRVLFDEVNIKFTHGNCYGVIGANGSGKSTFL
                     KILSGEIESTTGNIEFSPGERMSVLKQNHFEFDELSVLDTVMMGNKRLYDVMKEKDAL
                     YAKADFTDEDGIRVGDLEAEFAEMDGWNAESDAAEMLSNLGVKEDQHYKLMKELPSND
                     KIKVLLAQALFGNPDILILDEPTNDLDVNTIDWLEDFLADFKNTVIVVSHDRHFLDMV
                     CTHIVDIDFSKINLFTGNYTFWYETSQLLLKQRADKNKKNEAKKAELLDFIARFSANA
                     SKSKQATSRKKALEKLDIEQIKPSSRKYPGIFFKQNREAGDMILDVRQLSYDLEDGTA
                     LFKDVTFTVNKGDKIAFVSKDGLVISNFFKVLAGESKAKKGEINFGVTITKSYVPNDN
                     SAYFKNADLNLVDWLRQFSAEKDETFIRGFLGRMLFSGEESLKKCTVLSGGEKVRCMM
                     SKTMLEEANFLILDEPTNHLDLESITSLNNGLIDYAGTILFTSHDHTFTQTIANRIIE
                     ITPNGMLDKMMSYDDYLADPKVKEQRAELYGTLV"
     gene            3023..3994
                     /locus_tag="PLL59_09060"
     CDS             3023..3994
                     /locus_tag="PLL59_09060"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HMY23998.1"
                     /translation="MIHNFQSALCINNTHLPFGVYDDLISNVDTTVWDKQGLVTKKRR
                     TERKTWIFVGVFSPELIAGFAIADAGMVATAFSYFYSFKDGIFVEDKTTVPLGFPQSF
                     NPNLDSEWTLGNYSIKTINGKMTLQYLGKYKMYIEIENTKQGASIVAPSKGNRPFNFT
                     YKNVCVPVKVQINNGGISTYATSGNYGSIDFTKGFPPRETIWNWLSFIGKTESNKNIA
                     VNLVDRFNDNMENILWVDGRKILLSAATFSFNEPLDKTDWLIATKDGILNCHLSPKGA
                     RKENVNALVLKSKFIQPYGILDGTINIDGNVEKFTAFGVAEDHHAVW"
     gene            4190..5131
                     /locus_tag="PLL59_09065"
     CDS             4190..5131
                     /locus_tag="PLL59_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HMY23999.1"
                     /translation="MKTLLNYLKSKNYILYSLVLVLSFTACKQSATKSETTSVSGKKI
                     GVLLVNHGSRSTTWRKALMDFEDTVRDSIIAGAKVSGVKTAYMEYNEPSIATRLKEFD
                     KEAYSDVIIIPIFLTVSPHSFEDIPTIIGQKEDPESMEMLKIEKIERYTPKAKTHITP
                     LLDFTDILQKNVLRRAQALSKNGANEGLVLIGYGDETYDKEWGELFDKVAAFVKQNIG
                     ISEHSYGWCGHIANYNPDETTKAINKVLSKKSAAVVIPVLVAHDEMFQVKIIGGGIQK
                     VAGYKEKVFYKPDAILPDSNITSWVIAVSQEYANKIQ"
     gene            5149..5712
                     /locus_tag="PLL59_09070"
     CDS             5149..5712
                     /locus_tag="PLL59_09070"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HMY24000.1"
                     /translation="MKIRKLRRLNIATHRDLGFFFSTLIIVYCLSGIALNHINEWNPD
                     FIIHKQTIKIPQQHQRDNIGKTEIETYGKLVGESQYKIYDFPTKDQVKIYYNDASLHV
                     NFTTKEGVYESVSRRPLFYHSNVIHRNSVKGWNWVSDIFAIMLIIINITGLFILRGKY
                     GISGRGKWLMAAGTLPPLLVLLINELS"
     gene            complement(5833..6645)
                     /locus_tag="PLL59_09075"
     CDS             complement(5833..6645)
                     /locus_tag="PLL59_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24001.1"
                     /translation="MKNLTLLLLSLVLFTSVCIAQSNKVVLCESYDKTTGEASGINKN
                     WDVDSKTGSYVYIVYSQATNIKDKLMLYVDKKSTSGSYTAFDTQDFTFDPKVDKKKWA
                     MFDYKFTESGDYRISVMGSGDEPLASTYTNIGYMKENPSDKDKSTDNEDGDEYDTYYY
                     ENSTITFGDEVDNDANVKGEATEFKLINGKRDIMLKLEQDDDLKCKEVEVSVYYGDKY
                     DEEVSRETYSVESMTWNWLKFKISVKKVGKYVVDIYNDKDVFINSGYFEVIK"
     gene            6795..7775
                     /locus_tag="PLL59_09080"
     CDS             6795..7775
                     /locus_tag="PLL59_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HMY24002.1"
                     /translation="MNYLKLIRPLNLLIIALTMILFRVCIVAASPYKLFYISTVLSTL
                     EFALLVIITITVAASGYVINDIFDVEIDAINRPHRVIVGQQISETQAYNFYKLLCGIS
                     IFGSILLAILTKNIRLSMLPLLLLVVLNFYAHTFKKQLIVGNFLIALLSAFVIWLIAL
                     FETTVVSTDTDLQTQVKSGIAIAGFMYGLFAFLTTFMREIVKDLEDMEGDKAGDCNTI
                     PFAFGVVKAKIIVSGFSLIILMILSLFVWFFPSIQIKNVPVFIVILLILPLIFINILL
                     FIAKTPKSYNFVSNLLKIYMVLGIASLLYFRSGIGPYIFMQFANFIEKLL"
     gene            7784..8350
                     /locus_tag="PLL59_09085"
     CDS             7784..8350
                     /locus_tag="PLL59_09085"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HMY24003.1"
                     /translation="MLKPNKIILGSQSPRRVEILADAGFDVQIIKPKVAEHFPVEMNL
                     YKIPEYLSKLKMFDVYSYLGEDNTMIITADTMVIFNNKLVGKPKNKEQALRFLSLMNG
                     KQHHVVTGVTMRLNKLQISFSEQATVFFKDLSAEAIQQYIDNYETLDKAGAYNIQEYN
                     GVDHIDGEFFNVMGLPLKRVLQEIRNWK"
     gene            complement(8415..9410)
                     /locus_tag="PLL59_09090"
     CDS             complement(8415..9410)
                     /locus_tag="PLL59_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HMY24004.1"
                     /translation="MFPTIAKLQEKIFTVDDLNFEKVALEIFHFQYTHNLVYKQFVDY
                     LKTDINAIDSIEKIPFLPIDFFKSHTILINNATSQKLFESSGTTGMLNSKHYVADLRL
                     YEQSFEKGFQQYYGNIEDWIILALLPSYLERDTSSLVYMVNALIQKSKNKDAGFYLNN
                     LDELAAKLTQLSSNSYKKILLIGVTFALLDFAEQFPMDLSNISIMETGGMKGRRKEMT
                     REEVHQQLQAAFQVPTIHSEYGMTELLSQAYSFGNSIFQTPNWMRVLKRDIYNPMQVS
                     SNKGRGALNVIDLANIYSCAFIATQDIVNIRDNGYFEVLGRIDNSDIRGCNLMVS"
     gene            9429..9920
                     /locus_tag="PLL59_09095"
     CDS             9429..9920
                     /locus_tag="PLL59_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24005.1"
                     /translation="MNIKHALIIIITLIGLQACYKTPSYPIEPTVRFSAFDKPNEIYT
                     LDAGEGGNLIVEFTDGDGDLGKLNNQDSSSFVVYRNLRDTSFFHDNMYVIPVIPKKGT
                     TNAISGKIEIKLNDALFNSYLSYFVMKGISIDTFTYKLYITDRAGHRSNEIVTPPIIV
                     KIP"
     gene            10030..>10719
                     /locus_tag="PLL59_09100"
     CDS             10030..>10719
                     /locus_tag="PLL59_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY24006.1"
                     /translation="MKPITDVIVIGAGFSGLSAAYYLQQKGLSVRVLEVSSQVGGRAR
                     SDIMHGYTLDRGLHFYHNSTTELSKIIQLDKLQLKNIYPGYLLCYEGMFHLFTNPLYQ
                     TLDTLSTAMAKNATFTDKIRLFGLYAKLKTSTYNKLVKEAESSTYEYLSKNGFSKKLI
                     DSFFRPMLAANIFDYNLQASSRFSKLYLKSLFQDHIALPKNGIGSIAKAIADKLHDGT
                     ISYKTKVKRVSD"
BASE COUNT         3553 a         1688 c         1821 g         3657 t
ORIGIN      
        1 ctaatgtttc tttgtccaca ttttcaccga tagcagcttt tactaaatag ggtcctgcta
       61 agaaaatact tcccgtgcca tcgacaatga tactttcgtc acacataatt ggtaagtatg
      121 caccaccagc aacacaactg cccatgatgg ctgcaatttg cggaacgccc atcgatgata
      181 gttttgcatt gttgcgaaat acacgaccaa aattttcttt atctggaaaa atttcatctt
      241 gcattggcaa atatacgcca gcactatcca ccaaataaat aacaggtaat ctgttttcta
      301 tagcaatttc ttgtgctctt aaatttttct tgccagtaat cggaaaccaa gcacccgctt
      361 ttacggtagc atcgttagca acaatcatac actggcgtcc agcaacatat ccaattactg
      421 ctaccacacc gccactcgga catccacctt gctcggcata catgccgtca cctactaatg
      481 cgccaatttc tatgtattca ctgtttttat cgactaaata atcaatgcgt tctcttgctg
      541 tcagtttgtt tttagcatgc tccgcatcta ttttcttttg accaccacct aaatatattt
      601 tttgtaattt agattttaac tcatcaatcg ccaaacgcat tccgtcttca tttgtattga
      661 aatcaatatc tgtcatgcca taaaaataag aataaaacat gaaatgcaaa aaatgtaacg
      721 aaccttcata caattgcttt attttatatg gcatataatg atagcacata aaatgtaatt
      781 taactttatt ttcatttgaa atcctgaata atagtatatc tttgcagtcg ggcaagtctt
      841 atacgaccag ctcctgacaa atcctccagg gtcggaaggc agcaaaggta gttggttgag
      901 cggtgcgctg taagtaactt gccttttttt gtgcaatttc ttattttagt atatagctgc
      961 ttccaaatgt gaagcagttt ttttattttt attctatgcc aattccacag cctaataatc
     1021 cattacatgg cataacgcta gagaaaatgt tgcgttatct tttagcatac tatacttggg
     1081 aaggactata taaacaagtg cctgctaatt gttttaaaaa caatccttct attaagtcga
     1141 gcttgacatt tttgcgtaaa acaccatggg cacgtcagcg ccttgaaact atttatattt
     1201 atatcacacg agacggcgtc aatgaagagt ggttcgcatt tgaagagtaa gtttcttgtc
     1261 ggtttcttaa tgttaatact acttaagttg tgttgttcat ttaggactta cgctacaaat
     1321 attgtatctt tgcaaaaatt tttaaaatct tatgattact gttaataatt tatccttaca
     1381 atatggcaaa cgcgtattgt ttgatgaagt aaatataaag tttacacatg gcaattgtta
     1441 tggtgtgata ggtgcgaatg gttcaggaaa atctacattt ttaaaaattc tttctggcga
     1501 gattgaatca acaacaggaa atattgagtt ttctccaggc gagcgtatgt ctgttttgaa
     1561 acaaaatcac tttgagtttg atgaactaag tgtgttagat acagtgatga tgggcaataa
     1621 acgtttgtac gatgtaatga aagaaaaaga tgctttatat gctaaagctg attttacaga
     1681 tgaagatggc attcgtgttg gtgacttaga agcagaattt gctgaaatgg acggctggaa
     1741 tgcggaaagc gatgcggcgg aaatgttgag caacttaggt gtaaaagaag accaacatta
     1801 taaactcatg aaagagcttc catcaaacga caaaataaaa gtattgttgg cgcaagcatt
     1861 atttggcaat ccagatattt tgattttaga tgagcctaca aatgacctcg atgtcaatac
     1921 aatcgattgg ttagaagatt tcttggctga ctttaaaaac acggttatcg ttgtatcgca
     1981 cgatcgtcac tttttagata tggtgtgtac gcatattgtc gatattgatt ttagtaaaat
     2041 caatttattt acaggtaact atactttctg gtacgaaaca tcacaattgt tattaaaaca
     2101 gagagcagat aaaaacaaga aaaatgaagc taagaaagca gagttacttg attttattgc
     2161 tcgtttcagt gcgaatgctt ctaagtcgaa acaagcaact tctcgtaaaa aagctttaga
     2221 gaaattggat attgaacaaa tcaaaccatc gagcagaaaa tatcctggaa ttttctttaa
     2281 acaaaatcgc gaagctggcg atatgatttt agatgttcga caattatctt acgacttgga
     2341 agatggaaca gcactattta aagacgtaac atttaccgta aataaaggcg ataagattgc
     2401 atttgtttct aaagacggct tagtgatttc taattttttc aaagtattag ctggtgaatc
     2461 gaaagctaaa aaaggagaaa tcaattttgg tgtaacgatt acaaaatcat acgtgcctaa
     2521 tgacaattca gcttatttta aaaatgctga tttaaattta gtagattggc taagacagtt
     2581 ttctgcggaa aaagatgaga catttattcg cggtttctta ggcagaatgt tgtttagcgg
     2641 cgaggaatct ctaaaaaaat gtactgtact ttcaggtggc gaaaaggtga gatgcatgat
     2701 gagtaaaacc atgttggaag aagcaaattt tttaatttta gatgaaccga ctaaccactt
     2761 agacttagag tcgataacat cgcttaataa tggtttaatt gattatgcag gcactatctt
     2821 gtttacatca catgaccaca cgtttacaca aacaattgcg aatagaataa ttgaaattac
     2881 accgaatggc atgcttgata agatgatgtc ttatgacgat tatttagcgg atccaaaagt
     2941 gaaagaacaa cgcgcagaat tgtatggtac attagtgtaa ttaaactatt tgcagcactg
     3001 tttttatttg tatctttatt ttatgataca caattttcaa tctgctttat gtataaacaa
     3061 tacgcatctt ccatttggtg tgtatgatga tttaatttca aatgttgata ctactgtttg
     3121 ggacaagcaa ggtttagtta caaaaaaaag acgaacagaa cgtaaaacat ggatatttgt
     3181 tggtgttttt agtcctgaat taatagctgg atttgccatc gcagatgctg gtatggttgc
     3241 aacagcattt tcctattttt actcttttaa agatggcatt tttgtagaag ataagactac
     3301 tgttccatta ggatttcctc aaagttttaa tccaaattta gattcggaat ggacacttgg
     3361 taattattct attaaaacga taaatggcaa aatgacttta cagtatcttg gcaaatataa
     3421 gatgtatatc gaaatagaaa atacaaaaca aggtgctagt attgttgctc catcaaaagg
     3481 aaataggcca tttaatttta cttataaaaa tgtgtgcgtg ccggttaaag ttcaaatcaa
     3541 taatggaggt ataagcactt atgcaacaag tggtaattat ggttctattg attttacaaa
     3601 aggctttcct ccaagagaaa ctatttggaa ttggctttcc tttattggca aaacagaaag
     3661 caataaaaat atagctgtta atttagtaga tcgatttaat gataatatgg aaaatatact
     3721 ttgggtcgat ggtcgtaaaa tactattatc tgcagccaca ttttccttta atgaaccttt
     3781 agataaaaca gattggctaa tagccacaaa agatggtatt ttaaattgtc atctttctcc
     3841 aaaaggcgca cggaaagaaa acgtaaatgc tttagtttta aaaagtaagt ttattcagcc
     3901 ttatggaata ttagatggca ccattaacat cgatggaaat gttgaaaaat ttactgcgtt
     3961 tggcgttgct gaagaccatc atgcagtttg gtaattactt cagaaatctt atttcatttt
     4021 ttctacgtat tcattaatta aattaggtat tccaatttta cctcgattta ggtatttcat
     4081 ttaaagcata ctacacgtac ttttgtaatc aataattagt ctaaataaaa atagtttacg
     4141 cctgattatt tttgaatttt atgtaataac ctattaaatc tattagttga tgaaaacatt
     4201 attaaattat ttaaaatcaa agaattatat cttatattct ttggtgttgg tgctgagttt
     4261 tacagcttgt aaacagtcag cgacgaaatc agaaacgaca agtgtatctg gcaaaaaaat
     4321 cggtgtttta ttggtaaacc atggttcaag atcgactaca tggcgcaagg ctttgatgga
     4381 ttttgaagat accgtacgtg attctattat tgcaggagca aaagtgagtg gtgtgaaaac
     4441 tgcctatatg gaatataatg aaccctctat tgctactcgt ttaaaagaat ttgataaaga
     4501 agcttattct gatgtcatta ttattccaat atttttaact gtgagtcctc actcttttga
     4561 agatattcca actattattg gccaaaagga agatccagaa tcaatggaaa tgcttaaaat
     4621 tgaaaagatt gaacgataca cgccgaaagc gaaaacgcat ataacacctt tattagattt
     4681 tacagatatt ttacagaaaa atgtattgcg cagagctcaa gccttatcta agaatggcgc
     4741 aaatgaaggg cttgttttaa ttggatatgg tgatgagact tatgataaag aatggggcga
     4801 attatttgat aaggtagcag cattcgtaaa acaaaatatt ggcattagtg agcatagtta
     4861 tggctggtgt ggacatattg caaattataa tcctgatgaa acgacaaaag caatcaataa
     4921 agtattatca aaaaaatcag cagcagtggt cattccagtt ttggtagcac atgatgagat
     4981 gttccaagta aaaattattg gtggtggtat tcaaaaagta gctggctata aagaaaaagt
     5041 gttttataaa ccagatgcca tcttgccaga ttcaaatatt acgagttggg taatcgctgt
     5101 aagccaagaa tacgccaata aaattcaata ataattgtaa tggataagtt gaaaataagg
     5161 aagttaagac gattaaatat tgccacacat cgcgatttag gttttttctt ttctacatta
     5221 attattgtgt attgtctatc aggcattgca ttaaaccata ttaatgaatg gaatcctgat
     5281 tttatcatac ataaacagac aataaaaatt cctcagcaac atcagcgcga taatattggt
     5341 aaaacggaaa ttgaaactta tggtaagcta gtaggagagt cgcaatataa aatttatgat
     5401 tttccaacaa aagatcaagt caaaatatat tacaacgatg catcattgca tgttaatttt
     5461 acaacgaaag aaggtgtgta tgaaagtgta tcgcgaaggc cattgtttta tcactctaat
     5521 gttatccata gaaatagtgt aaaaggctgg aattgggttt ccgatatttt tgccattatg
     5581 cttatcatta ttaatataac aggcttattt attttaagag gaaaatatgg aatttcgggt
     5641 agaggtaaat ggttaatggc tgctggtact ttgcctccat tattagtatt attaattaat
     5701 gaattaagtt aattgcaata gcaataaaaa agagataaga atttattctt atctcttttt
     5761 tatgtattat attcaaatat tatattattg ctttgtatga taatacaagt aataaggtag
     5821 tatgcattat agttatttaa ttacttcaaa ataaccagag ttgataaata catctttgtc
     5881 attataaata tctaccacgt atttgccaac ttttttcacg ctgattttaa atttaagcca
     5941 attccatgtc atgctttcta cactatatgt ttctcttgaa acttcttcat catatttatc
     6001 accataataa acactcactt caacttcttt gcattttaaa tcatcatctt gttctaattt
     6061 tagcatgata tctctcttgc catttattaa tttaaactca gttgcttctc cttttacatt
     6121 agcatcgtta tctacttcat ctccaaatgt gatagtggaa ttttcgtaat agtaggtgtc
     6181 gtattcatca ccatcttcat tgtccgtact tttatcttta tcactcggat tttctttcat
     6241 gtaaccaata ttggtatagg ttgaagctaa aggttcatca ccacttccca tcacagagat
     6301 tctgtaatca cctgattccg taaatttgta atcaaacata gcccattttt tcttatctac
     6361 ttttgggtca aaagtaaagt cttgcgtatc gaaggcggta taactgccgc ttgtactttt
     6421 tttatctaca tacagcatta atttatcttt gatatttgta gcttgtgagt aaacgatata
     6481 gacatagctt cccgttttgc tgtcaacgtc ccagttttta ttgatgccag aagcttcacc
     6541 agtagttttg tcgtagctct cgcataaaac gactttattg ctttgtgcaa tgcagacaga
     6601 agtaaataat acaagagata atagtaaaag tgttaggttt ttcatacttg atttttttgt
     6661 aagtaacaca aatttaatac caaaatcaat ataaagaata cccaatgtat ggtattgttg
     6721 tccaataaat gaattaatgt atctttgctt cgcttttaat acagcctttt ttttatactt
     6781 atttctaata aaatatgaac tatttaaaac ttattcgtcc attaaattta ttaataatcg
     6841 cactcacgat gatattgttt cgtgtttgta ttgttgccgc atcgccatat aaattatttt
     6901 atatttctac ggtattgagc acactagaat ttgcgttatt ggtaataatt acgataactg
     6961 tagcggcaag tggttatgta attaatgaca tttttgacgt tgaaattgat gccataaata
     7021 gaccacatcg agtaatcgtt ggacaacaaa ttagtgaaac tcaagcatat aatttttata
     7081 aattgttatg tggcatcagc atttttggaa gcatactttt ggcaatactt acaaagaata
     7141 ttagactatc tatgttgcca ttgttgttgt tggtggtact taatttttat gcacatactt
     7201 ttaaaaaaca acttattgtc ggtaattttt taattgcttt attgtcagca tttgtaattt
     7261 ggttgatagc cttatttgaa acaacagtag taagcacaga cacagatttg caaacgcagg
     7321 taaaaagtgg tattgcaata gctggtttta tgtatggctt atttgctttt cttaccacat
     7381 ttatgcgcga aatcgtaaaa gaccttgaag atatggaagg cgataaggct ggcgattgca
     7441 atactattcc atttgcattc ggcgtcgtaa aagcaaaaat aattgtaagt ggtttttctt
     7501 taattattct aatgattttg agtttgtttg tttggttttt cccaagtatt caaataaaaa
     7561 atgtacctgt ttttatagtg attttactta ttttaccatt aattttcata aatattttac
     7621 tttttattgc aaaaacacca aaaagctata attttgttag taatttatta aagatttata
     7681 tggtgcttgg tattgcttcc ttgttgtatt ttcgaagtgg aattggtcca tatatattta
     7741 tgcagtttgc caattttatt gaaaagctat tgtaaaacaa agtatgctaa agccaaataa
     7801 aattatttta ggttctcagt cgccaagaag agtagagatt ttagcggatg ctggttttga
     7861 tgtgcaaatt atcaaaccaa aagttgctga gcattttcca gttgaaatga atttgtacaa
     7921 aattcctgaa tatttatcaa agttaaagat gtttgatgtg tattcttatt taggcgaaga
     7981 caatacaatg ataattactg ccgatacgat ggttattttt aataataaac ttgtcggcaa
     8041 acctaaaaat aaagaacaag cgctccgatt tctttctttg atgaacggaa aacaacatca
     8101 tgtagttaca ggagtaacta tgcgtttaaa taaattacaa atttcttttt ctgaacaagc
     8161 aactgtgttt tttaaagatt tatctgctga ggcaattcag caatacatag ataattacga
     8221 aacattagat aaagctggcg cttataacat acaagagtat aatggcgtgg accacatcga
     8281 cggcgagttt tttaatgtaa tgggtttgcc tttgaaaaga gtattgcaag aaataagaaa
     8341 ttggaaataa tttttttaaa tatggtatgt tgcaagatgg atgaaagcaa catcaactta
     8401 ttctctgcta acaactaaga caccatcagg ttacaaccac gaatatcgct gttgtcgatt
     8461 ctgcctaaca cttcaaaata gccattgtct ctaatgttta caatatcttg tgtagcaata
     8521 aaagcacatg aataaatatt agccaaatca ataacattta aagcaccacg acctttgtta
     8581 ctgcttacct gcattggatt gtagatgtcg cgtttcaaaa cgcgcatcca atttggtgtt
     8641 tggaaaatac tatttccaaa cgaatatgct tgtgaaagca gttccgtcat gccatattct
     8701 gaatgtatag taggcacttg aaatgccgct tgtaactgct gatgcacttc ctcgcgcgtc
     8761 atttctttgc gcctgccttt catgccgcca gtttccatga tgctaatatt ggataaatcc
     8821 ataggaaatt gctcggcaaa atcaagtaaa gcaaaagtta cgccaatgag taatattttc
     8881 ttataactat tactgcttaa ttgagtaagt tttgccgcta attcatcgag attattcaga
     8941 taaaatccag catctttatt ttttgatttt tgtattaatg cattcaccat atatacaagc
     9001 gatgaagtat cgcgctcaag gtatgatggc agtaatgcta atataatcca atcttcaatg
     9061 ttgccataat attgctgaaa tcctttttca aagctttgct catacaacct caaatctgca
     9121 acataatgtt tgctattcag catgcctgta gttccactac tttcaaacaa tttttgcgaa
     9181 gttgcgttat ttatcaatat agtatgtgat ttaaaaaaat cgattggcag aaatggaatt
     9241 ttttctatcg aatcaatagc gttgatatct gttttcaagt aatcaacaaa ttgcttgtac
     9301 actaaattat gcgtatattg gaaatgaaat atttcaagtg caactttttc aaagtttaaa
     9361 tcatcaacag taaagatttt ttcttgtaat tttgcaatcg taggaaacac agtacaaagg
     9421 taaacaaaat gaacattaaa cacgcgctga taattattat tacactgata ggtttgcaag
     9481 cttgttataa aacaccaagc tacccaattg aaccaactgt gcgtttttct gcatttgata
     9541 agccaaatga aatatatact ttagatgcag gtgaaggtgg caatctaatt gtcgaattta
     9601 cagatggcga tggcgattta ggaaagttaa ataatcaaga ttcttcttca tttgttgtat
     9661 atcgcaattt acgagatact tcattttttc atgataatat gtatgtcatt cctgttattc
     9721 caaagaaagg aacaacaaat gctataagtg gcaaaattga aataaaacta aatgatgctt
     9781 tgttcaattc ttatctttct tattttgtca tgaaaggcat ttctatagat acatttacct
     9841 acaaattata tattactgat agggcaggac acaggagcaa tgaaattgtt acgccgccaa
     9901 tcattgtcaa aattccttaa tgtattgatt atcaatataa gaaccttaat cataaacttc
     9961 ccttaatgct taattaatgt cttaactgag actgttgcat agtatttgct ctatttttgt
    10021 tttcccaaaa tgaaacccat tacagacgtc atagtaattg gagcaggctt tagtggcttg
    10081 tcagcagcat actaccttca acaaaaaggt ttatcagtgc gtgtgctgga agtttcatcg
    10141 caagttggtg gtagggctcg ctccgacatt atgcacggct atacattgga tagaggctta
    10201 catttttatc ataattctac gacagaatta agcaaaatta ttcaattaga taagctacaa
    10261 ttaaaaaata tctatcctgg ctatttgttg tgctacgaag gcatgttcca cttgtttaca
    10321 aatccattgt atcaaacttt agatacactt tctacagcaa tggcaaaaaa tgccactttt
    10381 acggataaaa ttagattgtt tggtttatat gctaaactta aaacatctac ttataataaa
    10441 ttggtgaaag aagcggaatc ttcaacatac gaatatttgt caaaaaatgg atttagcaaa
    10501 aaattaatcg attcattttt cagacctatg ctagccgcca atatttttga ttacaattta
    10561 caagcgtctt caagattttc taaattgtat ttgaaatcat tgtttcaaga tcatatagct
    10621 ctaccaaaaa atggtattgg ttcaattgca aaagccattg cagacaaatt acacgatggt
    10681 acaataagtt ataaaactaa agtaaaacgc gtaagcgat
//
LOCUS       DAOQQV010000087        10676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2242_length_10676_cov_7.309952, whole genome shotgun sequence.
ACCESSION   DAOQQV010000087 DAOQQV010000000
VERSION     DAOQQV010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2242_length_10676_cov_7.309952"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..2825)
                     /gene="uvrA"
                     /locus_tag="PLL59_09105"
     CDS             complement(15..2825)
                     /gene="uvrA"
                     /locus_tag="PLL59_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMY24007.1"
                     /translation="MSKGKKITVNRESNILIKGARTHNLKNVDVLIPRNKLVLVTGVS
                     GSGKSSLTIDTLYAEGQRRYVESLSSYARQFLNRMDKPDVDYIKGLSPAIAIEQKTSV
                     GTTRSTVGTLTEIYDYLRLLYAKIGKTYSPISGQVVQKQEVSDVVDYIKTLAPSTKLQ
                     ILSPLKEISKNKLDTLLQDGIQRLFIQNTLIKIEDINLQDITTINKSKEKYILIDRTT
                     ADPSEDNLYRLADSIALAFAEGDGECVVDIPTVSNKIFSNKFEADGISFEQVSAQFFN
                     FNSPYGACATCEGFGTILGIDPDLVIPNKLLSVYEDAVVCWKGEVMSAWKLQLIKGAA
                     KCQFPIHKAIADLSKEQYKMLWHGNEFFQGINAFFKDVESQTYKIQYRVMLSKYRGRT
                     DCNDCNGTRLRRDTDYVKISGKSIGELLQMTIKHLHEHFSQLADTTKSESALSKADQQ
                     IANRILIEINNRLQFMVDVGLDYLNLNRFSNTLSGGESQRINLTRTLGSNLTGSLYIL
                     DEPSVGLHPHDTGKLINILKYLRDLGNTVIVVEHEEEAIRAADYLVDVGPYAGTLGGE
                     IVFAGNANEIESNPNSLTTKYLKGELIIEVPKIRRKPINFIEIKGARQHNLKNINVKI
                     PLNALSVITGVSGSGKTTLIKQILFPYLKNQLEGFGGKPGLFNEIIGFKNKLKKVEFV
                     DQNPIGRSSRSNPVTYIKAYDVIRDLFAAQALSKIRAYQAKHFSFNVEGGRCDACEGE
                     GEQIVEMQFLADVHLQCEVCGGKRFKQEILEVNYNGKNIADILALTVDDAILFFKDKK
                     EVVARLQALQDVGLGYIHLGQSSSTLSGGEAQRVKLASFLGKGAARDPVLFIFDEPTT
                     GLHFHDINKLLKAFNALIEIGHAVLVIEHNIDVIKCADWIVDLGPKGGEEGGHLVFQG
                     TPENLIKNTESVTAKYLKGKLK"
     gene            2985..3560
                     /locus_tag="PLL59_09110"
     CDS             2985..3560
                     /locus_tag="PLL59_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMY24008.1"
                     /translation="MYSIVQSDNELLQRYINGEENALATLLKKYQRKIFSFIMMRVKD
                     RALAEDIFQDTFFKVIDSLKAGKYNEEGKFLPWVMMIANNLCMDHFRRNKRMPVMVDS
                     DSTAILNLVRFAEESKEDKLMREQTVQQIKELINLLPPDQKEVLLLRHYADLSFKEIA
                     DMTGTNINTALGRMRYALLNLRKMIGDKVIV"
     gene            complement(3610..4455)
                     /locus_tag="PLL59_09115"
     CDS             complement(3610..4455)
                     /locus_tag="PLL59_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HMY24009.1"
                     /translation="MSDITTRIITGIFFVIVMIGGVVLSEYSFLILFSLLVILCINEY
                     HGIIHNLLTSSSKWKSMYKILNTIFSVIIFGIVFMVASKKIPLISLSLVCGFPLTWLV
                     VEMYTKSDKPFYNVCLNSMGIFYLAIPLSSASLLAFHAGEYSWHYLLAIMIFAWSNDS
                     FAYLFGRLLGKHKLFERISPKKTWEGFIGGILGTVLFAYIIFLLFPKWTGYSVDLTIY
                     VILAVITSITSTYGDLAESMIKRNLQIKDTGTALPGHGGFLDRFDGLIFSLPACTLYL
                     TFFIK"
     gene            complement(4491..5426)
                     /locus_tag="PLL59_09120"
     CDS             complement(4491..5426)
                     /locus_tag="PLL59_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HMY24010.1"
                     /translation="MTENIVDEHVNITLNNKGKIQDYAFGTQLFTWFLVFGFCFVLAQ
                     MISGALIVAYYQSIDMHKIVADVYHLNALRFAQLLATIISFLLPAFIFSKLKSNTILS
                     YSAANIGFNPVFFIIVPMLIYTIYPSINFLFFVNKWIGIGNVNQSSQTEYKMLVDALL
                     KDDSVVVLMFNLLIIAIVPAIAEEWIFRGSLQRFLSEKISIHIAIVFSAILFSLIHFE
                     FSGFLPRIALGIFLGYLFYYSGSLWLCIFAHAVNNGTQVVLMYLNNKDIYRTDVDNPT
                     MPAMWEIAVYSIAFIGLWSIFLHFAQQRKSSTFVK"
     gene            5449..6438
                     /gene="dusB"
                     /locus_tag="PLL59_09125"
     CDS             5449..6438
                     /gene="dusB"
                     /locus_tag="PLL59_09125"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HMY24011.1"
                     /translation="MIKIGDIELGDFPLLLAPMEDVSDPPFRYVCKQYGADLMYTEFI
                     SSEGLIRHAAKSVQKLDIFDYERPIGIQIFGGEEDAMVQSAQIVEQANPNLIDINYGC
                     PVNKVVCKMAGAGILQDIPKMVRLTAQVVKATKLPVTVKTRLGWDDNSKNIVEVAERL
                     QDIGIKALSIHGRTRKQMYKGEADWTLIAKVKENPRMHIPIFGNGDIDSPQKALDYKN
                     KYGVDGIMIGRAAIGAPWIFNQIKAYLFEGKLIPTPRIEDKIETIKSHLQKSLEWKGN
                     ALGILEMRPHYSNYLKGLPNIKPFRHRLVTTNDIDEIYSILEEVLNEYAGIEV"
     gene            6565..7773
                     /locus_tag="PLL59_09130"
     CDS             6565..7773
                     /locus_tag="PLL59_09130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY24012.1"
                     /translation="MLLLLFISFLLTIFYVSIMLTYKNEWNDIEEVHFLKEDVETSVS
                     IIIPARNEAANIAHLLQDISQQIYPENLIEVIIIDDFSEDNTYDICLEFAAQFQYIKV
                     YKLAQLIAELSSSIAYKKRAIEAAVQLAKGKLIITTDADCRLQQHWIHSIVSFYEKEK
                     CALIAAPVGYYQDKTKFQHFQALDFCGMQAITGAMLRMDIFNMANGANLAYEKEAFLS
                     VNGYQHIDQKASGDDMFLVYKIAHKFPNRVQFLKNKAAIVLTQPMQNIADFLQQRFRW
                     TSKSAAYQDKRITIILGLIYLFVLSIWMNAFYFIGKATYSILFPDRTLVALIQFLPAF
                     FFLAMAIFQLLCKSIIDYIFLKSAARFFNREDLMQSFFISEVHHCWYIPFVGTFGNIL
                     SYKWKGRKLR"
     gene            7749..8867
                     /locus_tag="PLL59_09135"
     CDS             7749..8867
                     /locus_tag="PLL59_09135"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY24013.1"
                     /translation="MERQKTTLSAQKHILIAPLDWGLGHATRCMPLIQYLIQHNHHLH
                     IAGNGDSLLYLKDAFPTLTFHELPGYNITYSVGKNAAFHVLLQTPKIWKAIRDEHQAV
                     ANIVSRVNIDLIISDNRYGVRHDKVKSIFICHQIALQSPRPFQFMNAFFLQWHLQQIE
                     KFDELWIPDEANENNLSGSLSHGIEFKIPHSYIGIQSRYSNFIKQTSFIDNIDFSILI
                     VLSGVEPQRTYLENKLIQVFKQTNEKVLLVQGKTIAFTQSTIDKISIVSYLNSNDLFA
                     AFQKAKSIICRSGYSTIMDLAAIGKKAILIPAQGQTEQEYLAASLANKGFAVYCKQDK
                     LDIENALEKLNNINGFPSNITIQNPNFENVINTALLNI"
     gene            complement(8873..9691)
                     /gene="murI"
                     /locus_tag="PLL59_09140"
     CDS             complement(8873..9691)
                     /gene="murI"
                     /locus_tag="PLL59_09140"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HMY24014.1"
                     /translation="MNTNAQSPIGLFDSGIGGLTVANAILQQLPNESLVYFGDTAHLP
                     YGDKSADTIRYFTKTIGSFLLSKECKIIVIACNTASSVAFEMLQQLAGDKAHVFNVID
                     AVVDEVINRKYKNVGVIGTLGTVNSNAYKNKINAKNPNVNVHQLATPLLAPMIESGFV
                     DGQISHLVIEEYLNAPSLHDIDTLVLACTHYPLIKKEIEDFYAAKNKKVDVLATNEIV
                     AVHIKKFMIENGIVNYEKKGRNTFYVSDYTESFEKTTQLFYRESIDLIQMPLFK"
     gene            complement(9780..10340)
                     /locus_tag="PLL59_09145"
     CDS             complement(9780..10340)
                     /locus_tag="PLL59_09145"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMY24015.1"
                     /translation="MKKISAIIIVVIATVASVYAQKTTKLGYLNSSELISLMPETAKA
                     DSAVDKYAAELDALGQQMYLEYQKKATDAQAKAKTLSEEQLEIIGKELADLEKRITDF
                     QQSADQKIGTKKEKLYAPILQKANDGIKAVAKANAYTYIFDSAAGTLLFADESDDILP
                     LVKAHLKLPDPKPAPKPATAPATTPK"
     gene            complement(10401..>10676)
                     /locus_tag="PLL59_09150"
     CDS             complement(10401..>10676)
                     /locus_tag="PLL59_09150"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMY24016.1"
                     /translation="ELKKQREDEIVKAEDELKDLQKNRFGYNGDLFKKRQELIQPIQD
                     KVFDAVQKIAQMRTYDFIFDKASGGTAMLYTNPQYDVSEEVLKKLGM"
BASE COUNT         3444 a         1975 c         1769 g         3488 t
ORIGIN      
        1 ctataactta aacctcattt caactttcct ttcaaatatt ttgctgtaac actttcagta
       61 tttttaataa ggttttctgg tgtgccttga aatactaagt gaccgccttc ctcaccacct
      121 tttgggccaa ggtccacaat ccaatctgca catttgataa catcgatatt atgctcaata
      181 accagcacag catgtccgat ttctatcagc gcattgaatg ctttcaataa tttattaata
      241 tcgtgaaaat gcaaacctgt tgtaggctca tcaaaaataa aaagcactgg atctcgcgcg
      301 gcacctttac ctaaaaaaga tgccaatttc acgcgttgtg cttcaccgcc actcaatgta
      361 gaactacttt gaccgagatg aatataacct agccccacat cttgcagcgc ttgtaacctt
      421 gctacaactt ctttcttatc tttaaaaaac aaaatggcat catcaaccgt tagtgctaaa
      481 atatctgcaa tattttttcc attgtaattt acttctaaaa tttcttgctt gaatcgcttg
      541 ccgccgcaca cttcacattg caaatgcaca tcggctaaaa attgcatttc tacaatttgc
      601 tctccttcgc cttcgcaagc atcgcatctt cctccttcca cattaaacga aaaatgcttt
      661 gcttgataag cgcgtatctt tgacaatgcc tgtgctgcaa aaagatcacg tataacatca
      721 taagctttaa tgtatgttac tggattgctt cttgaacttc tgccaatcgg gttttgatcg
      781 acaaattcta cttttttgag tttgttttta aagcctatta tttcattaaa caaaccaggc
      841 tttccgccaa aaccttccag ctgatttttt aaatatggaa ataatatttg cttgattaat
      901 gttgttttac ctgagcccga aacgcctgta attacagaca atgcatttaa aggaattttg
      961 acgtttatat tttttaaatt atgttgacga gcacctttta tttcaataaa attgattggt
     1021 tttcttctga tttttggtac ttcaatgatt aattctcctt ttaaatattt cgtcgttaaa
     1081 ctatttggat tgctttcgat ttcattagca tttccggcaa atacaatttc gccaccaagc
     1141 gtaccagcat atggtccaac atcgaccaaa taatcagcag cgcgtattgc ttcttcttcg
     1201 tgttccacca caataactgt attgcctaaa tcacgtaaat attttaagat attaatgagc
     1261 tttcctgtat cgtgtggatg taagcctacg cttggttcgt cgagtatata caaggagcct
     1321 gtaagattgc tgcccagcgt tctcgtgaga tttatgcgtt gtgattcgcc accagaaagt
     1381 gtgttagaaa aacgatttaa gttcaaataa tctaaaccaa catcaaccat aaattgcagg
     1441 cgattattta tttctattaa aattctatta gcaatctgtt ggtctgcttt tgataaggca
     1501 ctttcagatt ttgttgtatc tgcaagttga ctaaaatgtt catgcaaatg tttaatcgtc
     1561 atttgtaata attcgccgat agacttgccg cttattttca cataatctgt atcgcggcgg
     1621 agacgtgtgc cattgcaatc gttacaatcc gttcgaccac gatattttga cagcatcaca
     1681 cgatattgaa ttttataagt ttgcgattca acgtctttaa aaaatgcatt aatgccttga
     1741 aagaattcat tgccatgcca tagcatttta tattgttctt ttgacaaatc ggcaattgcc
     1801 ttatgaattg gaaattgaca tttggcagca cctttaatga gttgcaattt ccatgcactc
     1861 atcacctcgc ccttccaaca aacaacggca tcttcgtaaa cagataaaag tttattaggt
     1921 ataaccaaat ctggatcaat gcctaaaatg gtgccaaatc cttcacaagt tgcacaggcg
     1981 ccatacggcg aattaaaatt aaaaaactga gcacttactt gttcaaagga gatgccatct
     2041 gcttcgaatt tgttggaaaa tattttattc gaaactgtcg gaatatcgac aacacattca
     2101 ccgtcacctt cagcaaatgc aagtgcaata gaatcggcga gcctgtataa attgtcttct
     2161 gaaggatcgg cagtggttct gtcaattaaa atatatttct ctttcgattt gtttattgtc
     2221 gtaatgtctt gaagattgat atcttctatt tttatcaaag tattttgaat gaacaaacgc
     2281 tgtatgccat cttgcaacaa agtatccaat ttgtttttgg aaatctcttt taatggcgat
     2341 aatatctgga gttttgttga aggtgctaat gttttaatat aatctacaac atctgacact
     2401 tcttgttttt gtactacttg tcctgaaata ggagaataag ttttaccaat ttttgcatat
     2461 aataatctca agtaatcata aatctctgtt aaagtgccca ctgtacttcg tgtggtgcct
     2521 acgcttgttt tttgttcgat ggcaatggca ggcgacaatc ctttgatgta atcgacatct
     2581 ggcttgtcca ttctatttaa aaattgacga gcatacgatg acaatgattc tacataccta
     2641 cgttgtcctt cagcatataa tgtgtcaata gttaaggaag attttcctga tccggaaaca
     2701 cccgtaacaa ggactaattt atttcttggt ataagcacat caacgttttt taggttgtgt
     2761 gtgcgcgcgc cttttatgag tatgttgctc tccctattta ccgttatttt ttttcctttt
     2821 gacatactac acagccttat tttgcgttaa atactgttaa tgataatacg ataatcgttt
     2881 ttgtttacta aacttgatat taattaacta accacataca atttaaaaat gaacaaactc
     2941 tacatgacaa cggcaaattt ttttacacgc aaaaaaaatg tatcatgtat tctattgttc
     3001 aatccgataa tgagcttctc caaagatata taaatggaga ggaaaatgca ctcgctacgc
     3061 tgctgaaaaa atatcaacgc aagatatttt cattcatcat gatgcgtgtt aaagaccgcg
     3121 cattggctga agatattttc caagatactt tttttaaagt tattgacagt ttaaaagctg
     3181 gtaaatacaa tgaagaaggc aagtttttgc cttgggtaat gatgattgcc aacaatttat
     3241 gtatggacca tttccgcaga aacaagcgca tgccggtgat ggtggacagc gacagtactg
     3301 ctattttaaa tcttgtacga tttgcggaag aaagcaaaga agataaattg atgcgcgagc
     3361 aaactgtaca acaaataaaa gagctgatta atttattgcc gcccgatcaa aaagaagtgc
     3421 ttctattgcg tcattatgcc gatttaagtt ttaaagaaat tgccgacatg actggcacca
     3481 atatcaatac tgcacttggt agaatgcgct atgctttatt aaacttacga aaaatgattg
     3541 gtgacaaagt aatagtgtaa tggagcttta tgctggtccg tctgtatcca cttcgattag
     3601 gcgataattc tatttaataa aaaaagtaag gtacaaagta cacgctggca aggaaaaaat
     3661 aagaccatcg aaacggtcta aaaatccacc atgaccaggc aaagcagtac ctgtgtcttt
     3721 aatttgcaaa ttgcgtttta tcatgctttc tgctaaatct ccataagtgc ttgtaatgga
     3781 agtaataact gctaaaataa cgtatattgt taaatcaaca gaatagccag tccacttcgg
     3841 aaatagcaga aaaatgatat aggcaaatag aacagttcct aaaataccgc caataaatcc
     3901 ttcccatgtt ttctttggcg atatacgttc aaacaattta tgtttgccaa gtaatctacc
     3961 aaataaataa gcaaatgaat cgttgctcca ggcaaaaatc ataattgcca acaagtagtg
     4021 ccaactatat tcgccagcat gaaatgccaa taagcttgct gaagaaagtg gaatagccaa
     4081 atagaaaatt cccatggagt ttaaacaaac attataaaac ggtttgtcgc ttttcgtgta
     4141 catttctacc actaaccatg tgagcggaaa accacatacc aaagacaacg atattaaagg
     4201 gattttttta ctcgccacca taaatacaat accaaaaatg ataacactaa aaattgtgtt
     4261 taagatttta tacatcgact tccatttcga agaagatgta agcagattat gaatgatacc
     4321 atgatattca ttgatacata aaatcactaa aagtgaaaaa agaataagga atgaatattc
     4381 gctaagcact acgccgccta tcattactat gacaaagaaa attcctgtta taattcgagt
     4441 ggtaatatcg ctcatacttt aattcattta gcaattagct aattggctaa ttattttaca
     4501 aaagtactac tttttctctg ttgtgcgaaa tgcaagaaaa tagaccacaa tcctataaat
     4561 gctatactgt acaccgctat ttcccacatc gctggcattg taggattatc gacatctgtt
     4621 ctataaatgt ctttgttatt caaatacatc aacaccactt gtgtgccatt atttacggcg
     4681 tgagcaaaga tacataacca caaactgcct gaataataaa ataaatatcc taaaaaaatg
     4741 cccaatgcta tgcgcggcaa aaatcctgaa aattcaaagt gaataagact aaacaatatg
     4801 gcagaaaaaa ctatagcgat atgtatagaa attttctccg acaaaaaacg ctgtaatgag
     4861 cctctaaaaa tccactcttc ggcaatagca ggaacgatag cgataatgag caaattaaac
     4921 attaacacca ctacgctatc atcttttaat aaggcatcga ccaacatttt gtattctgtt
     4981 tgtgatgatt gatttacatt gcctattcct atccatttat tgacaaaaaa caagaaattg
     5041 atacttggat aaatcgtgta aatcaacatg ggcactatga taaaaaaaac aggattaaat
     5101 ccaatatttg ctgcactata agaaagtata gtgtttgatt ttaatttgct aaaaataaat
     5161 gctggcaaca aaaaactaat tatcgttgcc aataattgtg caaagcgcaa agcattaaga
     5221 tgatagacat ctgccactat tttatgcata tcgattgatt gataataagc aactatcaat
     5281 gcaccactaa tcatttgcgc cagtacaaag caaaagccaa atacaagaaa ccatgtgaat
     5341 agctgtgtgc caaatgcata atcttggatt tttcctttat tgtttaatgt gatgtttacg
     5401 tgctcatcta ctatattctc cgtcatcgtt gtatctttgt gtaaagatat gataaaaatc
     5461 ggcgacatag aattaggtga tttcccattg ttgttagcac caatggaaga tgtgagcgat
     5521 ccgcctttta gatatgtttg caaacaatat ggcgccgacc tcatgtacac tgagtttatt
     5581 tcgtctgaag gcttaatcag acatgctgca aaaagtgtcc aaaaattaga catcttcgat
     5641 tatgagcgac ccataggcat tcagattttt ggtggcgaag aagatgcaat ggtacaaagt
     5701 gcacaaattg tagaacaagc caatccaaac cttatcgata tcaattatgg ctgtcctgtc
     5761 aataaagtcg tttgcaaaat ggctggcgcc ggcatcttgc aagatattcc gaaaatggtg
     5821 cgactaacag cacaagttgt caaagcaaca aaattaccag taactgttaa aactcgctta
     5881 ggctgggacg ataattcgaa aaatattgta gaagtggcag aacggctgca agacatcggc
     5941 atcaaagcac ttagcattca cggtcgcacg cgcaaacaaa tgtataaagg cgaggccgat
     6001 tggacactaa ttgccaaagt aaaggaaaat ccaagaatgc atattcctat ttttggaaat
     6061 ggcgatatcg actcaccaca aaaagcactg gattacaaaa ataaatatgg cgttgatggc
     6121 attatgattg gccgtgctgc catcggtgcg ccttggattt ttaatcaaat aaaagcgtat
     6181 ttatttgaag gaaaattaat tccaacacca cgcattgaag acaagattga aaccataaaa
     6241 tcgcatttac aaaaatcgct tgaatggaaa ggcaatgcac tcggtatatt agaaatgcgt
     6301 cctcattatt ccaactattt aaaaggatta ccaaatataa aacctttcag acatcgattg
     6361 gtgactacaa atgacatcga tgaaatatat tctatattag aagaagtact gaatgaatat
     6421 gctggcattg aagtttaatc attttacatt ttaaataagc aaaaagtagt aatacgaata
     6481 acacaagtgt ataaaaagta attctattac aaataggaat gcttaacttt aaacatcgca
     6541 taagaaaaac caaaactatc tatcgtgttg ctattactgt ttatttcatt tttactcact
     6601 attttttatg taagcatcat gcttacttat aaaaacgaat ggaatgacat tgaagaagta
     6661 cactttttaa aagaagatgt cgaaacaagc gtaagcatta tcattccagc acgaaatgaa
     6721 gccgcaaata ttgctcattt attacaagat atttcacagc aaatatatcc tgaaaactta
     6781 atagaagtta tcattatcga tgatttttct gaagataaca cctacgatat ttgtctcgaa
     6841 tttgccgcac aattccaata cattaaagtc tataaactag cacaacttat tgcagaacta
     6901 tcgtcttcaa ttgcctataa aaaaagagca attgaagcag ctgtgcaatt agcaaaaggg
     6961 aaactcatca tcacaacaga tgcggattgc agattgcagc aacattggat tcatagcatt
     7021 gttagttttt atgaaaaaga aaaatgtgct ttaattgctg cgcctgttgg ctattatcaa
     7081 gacaaaacaa aatttcaaca ttttcaggca ttagattttt gtggaatgca agcaattact
     7141 ggtgctatgt tgcgcatgga tattttcaat atggcaaatg gcgctaattt agcttatgaa
     7201 aaagaagcat ttttatcggt aaatggctat caacatatcg accaaaaagc cagtggcgac
     7261 gatatgttct tagtgtacaa gattgctcat aaattcccaa atcgtgtaca atttttaaag
     7321 aacaaagcag ccattgtgct tacgcaacca atgcaaaata ttgctgattt tttgcaacag
     7381 cgttttagat ggactagtaa gtctgctgct taccaagaca agcgtatcac tattatctta
     7441 ggattgattt atttatttgt acttagcatt tggatgaatg ccttttattt tattggcaaa
     7501 gcaacttatt ctatcttatt tcctgacaga actttagtgg cacttattca attcttgcct
     7561 gccttctttt ttttagccat ggctatcttt caattgttgt gtaaatctat tatcgattat
     7621 atttttttga aatctgctgc acgcttcttt aatagagaag atttgatgca atcttttttc
     7681 atcagtgaag tgcaccactg ttggtatatt ccatttgtag gaacatttgg aaatatatta
     7741 tcttacaaat ggaaaggcag aaaactacgt tgagcgcaca gaaacacata ctcatcgcgc
     7801 cattagattg gggcttaggg cacgccacta ggtgcatgcc gttaattcaa tatttaattc
     7861 agcataatca tcacttacat attgctggta atggcgattc attattatac ttaaaagatg
     7921 catttccaac actcaccttt catgagttgc ctggctacaa tatcacctat tctgttggaa
     7981 aaaatgctgc atttcatgtc ctgttgcaga caccaaaaat atggaaagca attcgcgatg
     8041 aacatcaagc cgtagcaaac attgtgtcaa gagtaaatat cgatttaatc atctccgaca
     8101 accgatatgg tgttcgacat gataaagtga aaagtatttt tatctgccat caaattgctt
     8161 tgcaatcacc acgaccattt cagtttatga atgccttttt cttacaatgg catttacaac
     8221 aaattgaaaa attcgacgag ttgtggattc ctgacgaggc caatgaaaac aacctttctg
     8281 gcagtttatc acatggaatt gaatttaaga ttccgcattc ctacattggc atacaaagtc
     8341 gatattctaa tttcatcaaa caaacttcat ttattgacaa tattgatttt tccatactga
     8401 tagtgctgtc tggtgtggag ccgcaaagaa cttatttaga aaacaaattg atacaagtat
     8461 tcaagcaaac gaatgaaaaa gtattgttgg ttcaaggtaa aactatagca tttacacaaa
     8521 gcactattga caaaataagc atcgtttctt acttaaattc taatgattta tttgctgctt
     8581 ttcaaaaagc aaaaagtatc atttgtcgct caggctattc taccatcatg gacttagctg
     8641 ccattggaaa aaaagcaatt ctcattcctg cacaaggcca aaccgaacag gaatatttgg
     8701 ctgcatcatt agcaaataaa ggcttcgccg tatattgtaa gcaagacaaa ttagatatag
     8761 aaaatgcatt ggaaaaatta aataacatca atggttttcc ttcaaacata actatacaga
     8821 atccgaattt tgaaaatgta ataaatacag cattgctcaa tatttgaacc tatcatttaa
     8881 acaaaggcat ctgaattaaa tcgattgatt ctctataaaa aagctgtgtc gttttctcaa
     8941 aactttccgt atagtctgac acataaaatg tatttctacc ttttttttcg taattgacaa
     9001 tgccattttc aatcataaat tttttgatat ggacagcaac aatttcattg gttgccaata
     9061 catctacctt tttattttta gctgcataga aatcttcaat ttcttttttt attaaaggat
     9121 aatgcgtgca tgccaacacc aatgtgtcta tatcatgcaa gctgggtgca tttaaatatt
     9181 cttcaataac taagtgagaa atttgtccat ctacaaatcc tgattctatc attggcgcaa
     9241 gcaatggcgt tgccaattga tgcacgttaa cgttgggatt tttagcatta attttatttt
     9301 tataggcatt tgaattaaca gtgcctaatg tacctattac gcctacattt ttatattttc
     9361 tatttatcac ttcatcgaca acggcatcaa tcacattaaa cacatgtgct ttatcgcctg
     9421 ctaattgttg tagcatttca aacgcaacag aagatgccgt attacaagca atcacgataa
     9481 ttttgcattc tttagataat aaaaaagaac cgattgtttt ggtaaaataa cgaattgtat
     9541 ccgctgattt atcgccgtat ggcaagtgtg ccgtatcgcc aaaatacact aaagattcgt
     9601 ttggcaattg ttgtaaaatg gcatttgcca ccgtgaggcc gccgatgcca gaatcaaata
     9661 aaccaattgg cgactgtgca tttgtattca tgtaacaaag ttgagaaact tattgataaa
     9721 aacaaaaaaa gttcgtcttg cctgcggcat gacgaacttt tgattaattg tctaaataat
     9781 tatttaggtg ttgttgctgg cgctgttgct ggttttggcg ctggctttgg atctggcaat
     9841 tttaagtgtg cttttaccaa tggtaagata tcatcagatt catcagcgaa taatagtgtt
     9901 ccggctgctg aatcaaaaat atatgtgtaa gcatttgctt ttgctaccgc tttgattcca
     9961 tcgtttgctt tttgtaaaat tggtgcgtat aatttttctt ttttagtgcc aattttttgg
    10021 tctgctgatt gttggaaatc cgtaatacgt ttttctaaat ccgctaattc tttaccgata
    10081 atttctaatt gctcttcaga taaagtttta gcttttgctt gtgcgtcagt cgctttcttt
    10141 tgatattcca aatacatttg ttgacctaat gcatctaatt ctgctgcata tttatctact
    10201 gcagagtctg cttttgccgt ttctggcatt aaactgatta attcagaaga atttaaatag
    10261 ccaagttttg ttgttttttg tgcatacacg ctcgctactg ttgcgatcac cacgataata
    10321 attgctgaaa tttttttcat cttgttgtta tttaatttat tgtttggttt aagtttttta
    10381 ctttttattt actattcaaa ttacatgcct aattttttga gtacttcttc gctcacgtcg
    10441 tattgcggat ttgtatatag catggctgtg cctccagatg ctttatcgaa gataaaatca
    10501 taagtgcgca tttgtgcaat cttttgaacc gcatcaaata ctttatcttg gataggctgt
    10561 atcaattctt ggcgcttttt gaacaaatcg ccattatagc caaatctatt tttttgcaag
    10621 tcttttaatt cgtcttcggc tttcactatt tcgtcttctc gctgtttctt caattc
//
LOCUS       DAOQQV010000088        10660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2249_length_10660_cov_7.643281, whole genome shotgun sequence.
ACCESSION   DAOQQV010000088 DAOQQV010000000
VERSION     DAOQQV010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2249_length_10660_cov_7.643281"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /gene="rplQ"
                     /locus_tag="PLL59_09155"
     CDS             complement(<1..409)
                     /gene="rplQ"
                     /locus_tag="PLL59_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HMY24017.1"
                     /translation="MRHGNKINHLGRKYGHRAALLKNLAAALFVHKRIETTVAKAKEL
                     RGYVEPLVTKAKDNTTHSRRTAFSYLQDKEATKELFDVIAPKVGNRPGGYTRIVRLGQ
                     RLGDAAEMVIIELVDFNETYVTKATKAAEPKKKT"
     gene            complement(430..1425)
                     /locus_tag="PLL59_09160"
     CDS             complement(430..1425)
                     /locus_tag="PLL59_09160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HMY24018.1"
                     /translation="MAILSFQKPDKILLQKATDFEGTFEFAPLEPGFGNTIGNALRRV
                     LLSSLEGYAITNIKIAGVNHEFSTIKGVIEDVTEIVLNIKQVRLKKLMQDADAGYEKI
                     YISIKNKEQFTAGDIESHTNVFAVTNPDLVICNMEKNVNLELEISIGKGRGYVAAEEH
                     YVDNSIVGLIPVDSIYTPIKNVRYKIENTRVEQRTDFEKLIIDVKTDGTIHPEDAVKE
                     AAKILIQHLMLVSDENITFDSEEKGQEEVVDEHILHMRKILKTPLEDLDLSVRAYNCL
                     KAAKINSLSELVQYDTNELLKFRNFGRKSLVEIEELIIEKGMHFGMDLSKFKLED"
     gene            complement(1490..2098)
                     /gene="rpsD"
                     /locus_tag="PLL59_09165"
     CDS             complement(1490..2098)
                     /gene="rpsD"
                     /locus_tag="PLL59_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HMY24019.1"
                     /translation="MARYTGPRTKKSRAFGEPIFGYDKAFEKRKYAPGQHGSSRKRST
                     KSEYAIQLKAKQQAKYTYGVLERQFYKTFAEAARKHGVTGENLLRLLEQRLDNTVFRL
                     GFAPTRSAARQFVNHKHITVNGKILNIPSYTVKVGDVISIREKSKNLDIVQDTLKLGG
                     VKKYGWLELNAEKLEGKYVELPNRDQIPETIKEQLIVELYSK"
     gene            complement(2162..2554)
                     /gene="rpsK"
                     /locus_tag="PLL59_09170"
     CDS             complement(2162..2554)
                     /gene="rpsK"
                     /locus_tag="PLL59_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMY24020.1"
                     /translation="MAQTTKKTAKKRVVKVEAEGYVYIKATFNNIIVSYTNKAGQVIS
                     WSSAGKCGFRGSKKSTPYAAQMAAQDAGTKAYEAGLRRAEVFVKGPGGGRESAIRTIG
                     NAGIEVTIIRDVTPLPHNGCRPPKKRRV"
     gene            complement(2578..2955)
                     /gene="rpsM"
                     /locus_tag="PLL59_09175"
     CDS             complement(2578..2955)
                     /gene="rpsM"
                     /locus_tag="PLL59_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HMY24021.1"
                     /translation="MARVSGIDLPKNKRGYIGLTYIYGIGRSTALAILNTAGVDPQKK
                     VHEWNDDEATAIRNAITAEYKVEGQLRSETQLNIKRLMDIGSYRGIRHRKGLPVRGQS
                     TKRNARTRKGKRKTVAGKKKVTK"
     gene            complement(2960..3076)
                     /gene="ykgO"
                     /locus_tag="PLL59_09180"
     CDS             complement(2960..3076)
                     /gene="ykgO"
                     /locus_tag="PLL59_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HMY24022.1"
                     /translation="MKVKASVKKRSEDCIIVRRKGKVYVINKKNPKFKQRQG"
     gene            complement(3094..3312)
                     /gene="infA"
                     /locus_tag="PLL59_09185"
     CDS             complement(3094..3312)
                     /gene="infA"
                     /locus_tag="PLL59_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HMY24023.1"
                     /translation="MGKTDLIKLDGTITEALSNAMFRVKLENGHEILATISGKMRMNY
                     IRILPGDKVAVEISPYDLSRGRITFRYK"
     gene            complement(3341..4141)
                     /gene="map"
                     /locus_tag="PLL59_09190"
     CDS             complement(3341..4141)
                     /gene="map"
                     /locus_tag="PLL59_09190"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMY24024.1"
                     /translation="MIHLKTPEEIENIRKSSLLVSQTHALLVEYIKPGVKTAKLDEIA
                     EQFIRDNGATPSFKGYGGFPASLCISVNEQVVHGFPSDREIKEGDVLSVDCGVYKDGF
                     HGDSAFTFPIGEVSAESKKLLQVTFNSLYLGIEQAIAGNRIGDIGFAIQNYCENTHNY
                     GVVRDLVGHGIGKDLHEDPQVPNYGKRGKGPMLQEGMVLAIEPMINMGTYQVRTLSDK
                     WTIIARDGKPSAHFEHTLAVKRGKADILSDHNLIFEAIKKSEYIKDFR"
     gene            complement(4155..5489)
                     /gene="secY"
                     /locus_tag="PLL59_09195"
     CDS             complement(4155..5489)
                     /gene="secY"
                     /locus_tag="PLL59_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HMY24025.1"
                     /translation="MKQFFDTLKNIFSIEDLRKRIFLTLGLIFIYRLGCFVVLPGVDP
                     TKISSGGASGIFQIFDLFAGGAFTKASVFALGIMPYISTSIVMQLLTLAVPYFQKLQK
                     EGESGRKKITQYTRYGTVAVTAFQGFAYVKFLYSQNIVSPEISTTLFSLSTMIILITG
                     SVFVMWLGEQIQERGLGNGTSLIIMANIISRLPASFWAEAKGRAVAGGSGILMFVLEM
                     VVLAFVIIATIMLIQAIRKVPLSYAKQMSGGNNAYTQRRGFLPLKVNIAGVMPIIFAQ
                     AFLFIPGTIAQMFPDSSASGFLRQLTDFTSLPYNIIMFLMVVLFTYFYTALIMNPVQM
                     ADDLKRNNGFIPGVKPGANTAEYIDTVLSRITLPGSIFLGFIAIMPSFANVLGVNSQF
                     AYFFGGTSLLILVSVVLDTMQQINSHLLSRHYDGLTKSGAKIRGRNVATSDY"
     gene            complement(5497..5952)
                     /gene="rplO"
                     /locus_tag="PLL59_09200"
     CDS             complement(5497..5952)
                     /gene="rplO"
                     /locus_tag="PLL59_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HMY24026.1"
                     /translation="MSISLNNLKPAKGAVKGRKRIARGQGSGSGGTAGKGHKGAKSRS
                     GYKSKRGFEGGQMPLQRRLPKRGFKNPNRVEFRTINLAELQALAEKNNITEFTFETYR
                     TLKLVKASDKIKVLANGELKGALKVSAHAFSDKAKEAIEKNSGAATVIA"
     gene            complement(5955..6134)
                     /gene="rpmD"
                     /locus_tag="PLL59_09205"
     CDS             complement(5955..6134)
                     /gene="rpmD"
                     /locus_tag="PLL59_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HMY24027.1"
                     /translation="MAKIKITQVKSTIDRSQRQKDTIKALGIQKLNRSVEKEATPQVL
                     GMVAKVKHLVTVENI"
     gene            complement(6139..6660)
                     /gene="rpsE"
                     /locus_tag="PLL59_09210"
     CDS             complement(6139..6660)
                     /gene="rpsE"
                     /locus_tag="PLL59_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HMY24028.1"
                     /translation="MSKVKENKVRTATEIELKEKVVGIQRVAKVTKGGRTFSFSALVV
                     VGDGNGTVGHGLGKAREVQEAIQKGIDDAKKNLVKIPILKGTIPHDQAAEFDGAKVLI
                     KPASHGTGVIAGGSMRAVLESAGISDVLAKSLGSANPHNVVKATIQALTLLRDPFTVA
                     RDRGIKLTKVFNG"
     gene            complement(6704..7048)
                     /gene="rplR"
                     /locus_tag="PLL59_09215"
     CDS             complement(6704..7048)
                     /gene="rplR"
                     /locus_tag="PLL59_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HMY24029.1"
                     /translation="MAVTNKLSRREKIKFRIRKKISGTTERPRLCVFRSNSEIYAQII
                     DDATGKTIASVSSQKSTGTKTEKAKAVGASIASKAKDANIANVIFDRNGFLYHGRIKA
                     LADAARENGLQF"
     gene            complement(7104..7661)
                     /gene="rplF"
                     /locus_tag="PLL59_09220"
     CDS             complement(7104..7661)
                     /gene="rplF"
                     /locus_tag="PLL59_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HMY24030.1"
                     /translation="MSRVGKLPISIPAKTEVNVADNNVVTVKGPKGTLSEKVDRDIIV
                     KVENGNVVLERPTDQKRHRALHGLYRALLNNMVLGASEGYKKSLDVVGVGFRASNQGQ
                     LLELSIGFSHPIFVLIPEEVKVSTVSEKGQPPRIILESTDKQLIGQVAAKLRSLRKPE
                     PYKGKGIKYTGEILRKKAGKSAGKK"
     gene            complement(7674..8072)
                     /gene="rpsH"
                     /locus_tag="PLL59_09225"
     CDS             complement(7674..8072)
                     /gene="rpsH"
                     /locus_tag="PLL59_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HMY24031.1"
                     /translation="MVTDPIADFLTRIRNAQLAGHTIVDIPASNMKKKLTEILHENGY
                     ILKFKFEEDGPQGTIKIALKYNPETREPAIRELVRISKPGLRRYAHVAGIPRVKNGLG
                     LAILSTSRGILTDKQAKNLNVGGEIICIVS"
     gene            complement(8172..8441)
                     /gene="rpsN"
                     /locus_tag="PLL59_09230"
     CDS             complement(8172..8441)
                     /gene="rpsN"
                     /locus_tag="PLL59_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HMY24032.1"
                     /translation="MAKESMKARDAKRLKLINKYKAKRDELKKAGDYTALDKLPKNAS
                     PVRYRKRCKLTGRPRGYIGFFGLSRIQFRDLASYGMIPGLSKASW"
     gene            complement(8444..8995)
                     /gene="rplE"
                     /locus_tag="PLL59_09235"
     CDS             complement(8444..8995)
                     /gene="rplE"
                     /locus_tag="PLL59_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HMY24033.1"
                     /translation="MSYIPTLKKKYTEEVVPALKTKYNYKSVMQVPKLTKIVVNQGVG
                     AAVGDKKLIDNSIDEISRITGQKAVSTKSKKAISNFKLRENLPIGVRVTLRGERMYEF
                     LERLMAVALPQVRDFKGINDKSFDGRGNYTLGITEHIIFPEIDIDKISKISGMDITFV
                     TSANTDDEAYSLLKEIGLPFKKK"
     gene            complement(8995..9333)
                     /gene="rplX"
                     /locus_tag="PLL59_09240"
     CDS             complement(8995..9333)
                     /gene="rplX"
                     /locus_tag="PLL59_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HMY24034.1"
                     /translation="MSTSTQKRFAPKLKIKKGDTVIVITGEDRGKSGKVLEVFPSKNT
                     ALVAGVRIISKHTKPNAQNQQGGIVKKEAPINISNLMLSVGGKATKVGRKVEDGKSVR
                     IAKSTGEIIK"
     gene            complement(9355..9723)
                     /gene="rplN"
                     /locus_tag="PLL59_09245"
     CDS             complement(9355..9723)
                     /gene="rplN"
                     /locus_tag="PLL59_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HMY24035.1"
                     /translation="MVQQESRLRVADNSGAKEVLCIRVLKGTRARYASIGDKIVVSVK
                     DAMPGGNVKKKQVSKAVVVRTKKEIKRKDGTYIRFDDNAVVLLNNNDEPRGTRIFGPV
                     ARELRDKDYMKIISLAPEVL"
     gene            complement(9725..9997)
                     /gene="rpsQ"
                     /locus_tag="PLL59_09250"
     CDS             complement(9725..9997)
                     /gene="rpsQ"
                     /locus_tag="PLL59_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HMY24036.1"
                     /translation="MTTELTTERQLRKTRVGIVTSNKMEKTITVAVVRKLKHPKYGKF
                     LKKTKKFHAHDEKNECNIGDTVKIMETRPLSATKRWRLVEIVEKAK"
     gene            complement(10009..10221)
                     /gene="rpmC"
                     /locus_tag="PLL59_09255"
     CDS             complement(10009..10221)
                     /gene="rpmC"
                     /locus_tag="PLL59_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HMY24037.1"
                     /translation="MATTVAYKEITSWSDEELNNELSASKLKLTKLKFNHAITPLENT
                     NVLGETRKHIARIQTELRKRKLSKQA"
     gene            complement(10233..>10660)
                     /gene="rplP"
                     /locus_tag="PLL59_09260"
     CDS             complement(10233..>10660)
                     /gene="rplP"
                     /locus_tag="PLL59_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HMY24038.1"
                     /translation="LQPKRVKFRRVHKMKMKGIAYKGSQLSFGSFGLKSLDECWITDR
                     QIEAARIALTRNMKREGKVWINIFPDKPITKKPAEVRMGKGKGSLDHWVAVVKPGRIL
                     FEIEGVSKELAEESLRLAAQKLPVLTKFIVRRDYDAANQ"
BASE COUNT         2806 a         2064 c         1745 g         4045 t
ORIGIN      
        1 gtgttttttt cttaggttca gcagctttag ttgctttagt aacatatgtt tcgttgaagt
       61 caaccaattc aatgattacc atttctgcag catcgcctaa acgttgacct aagcgaacga
      121 tacgcgtgta accaccaggt ctgttgccca cttttggtgc aatcacatca aacaattctt
      181 ttgtagcttc tttatcttgc agataagaga aagcagtacg acgtgaatgt gttgtattat
      241 cttttgcttt tgtaactaat ggttcaacgt aacctcttaa ttcttttgct ttagcaacag
      301 ttgtttcaat gcgtttatga acgaataaag ctgcagctaa atttttcaat aatgcggctc
      361 tgtgtccgta ttttctgcct aagtgattga ttttatttcc gtgtctcatg gcgacagtag
      421 tttatttttt tagtcttcta atttaaattt agaaaggtcc ataccaaaat gcataccttt
      481 ctcgatgatt aattcttcga tttcaactaa tgatttacga ccgaagtttc tgaatttcaa
      541 taactcattt gtgtcatatt gtaccaactc tgataatgag ttaatttttg ctgcttttaa
      601 gcagttatat gcacgaacag aaaggtctaa atcttccaat ggtgttttaa ggattttgcg
      661 catgtgtaag atatgctcat ctactacttc ttcttgacct ttttcttctg agtcgaatgt
      721 gatattttcg tcagaaacca acattaaatg ctgaatcaaa attttagcag cttctttaac
      781 agcatcttct ggatggatgg tgccgtcagt tttaacgtct atgattaatt tttcgaagtc
      841 agtacgttgc tccacacgag tattttcgat tttgtaacgt acgtttttaa taggtgtata
      901 aatagagtct actggaatta atccaacgat agaattatct acataatgtt cttcagcagc
      961 aacgtaacct ctacctttac cgatagaaat ttctaactcc aagtttacat ttttttccat
     1021 attgcaaatc actaaatcag gatttgtaac cgcaaatacg tttgtatgtg attcgatatc
     1081 gcctgctgtg aactgttctt tatttttgat agagatgtag attttttcgt aaccagcatc
     1141 tgcatcttgc attaattttt tcaaacgtac ttgtttgatg tttaatacga tttctgtaac
     1201 gtcttctatt acacctttga tagtagaaaa ttcgtggtta acaccagcaa ttttaatgtt
     1261 tgtgattgca taaccttcta aagaagataa caatactctg cgcaatgcat taccgatagt
     1321 atttccaaaa ccaggttcta gcggtgcgaa ttcaaatgtt ccttcgaagt cggtagcttt
     1381 ctgtaaaaga atcttatccg gtttttgaaa tgataaaata gccataatta atatttattt
     1441 ttaaagtttc aatctgaatg tttaattgta aaacattctc ttcaaaaaat tatttagagt
     1501 ataactcaac gattaattgt tctttaatag tttctgggat ttgatctcta tttggaagct
     1561 caacatattt accttccaat ttttcagcgt tcaactctaa ccagccatat ttttttacac
     1621 cacctaactt caatgtatct tgaacaatat ctaagttttt agatttttca cgaatgctaa
     1681 ttacatcacc cacttttact gtatatgatg gaatgttcaa tattttacca ttcactgtga
     1741 tgtgtttgtg gttaacaaat tgacgagctg cgctacgtgt aggtgcaaaa cctaaacgga
     1801 aaacagtgtt gtctaagcgt tgttctaaca aacgtaataa gttttcacca gtaacaccgt
     1861 gtttacgagc tgcttccgca aatgttttat aaaactgacg ctctaataca ccgtatgtat
     1921 attttgcttg ttgttttgct tttaactgaa ttgcatattc agattttgtc gaacgcttac
     1981 gagagcttcc gtgttgacct ggagcatatt ttcttttttc gaatgcttta tcgtatccga
     2041 agataggctc tccaaatgct ctcgattttt ttgtacgtgg tcctgtatat cttgccattt
     2101 ctttttaaga ttttagattt aagaattgag atttatttca aggcttaaac tatttttatt
     2161 attatactct acgtttttta ggaggacgac aaccattgtg tggcaatggt gttacgtcgc
     2221 gaataatggt tacctcaata ccggcattac cgattgttcg gatagctgat tctctgccac
     2281 caccaggtcc tttaacgaaa acttctgctc ttcttaaacc agcttcgtaa gctttagttc
     2341 cagcatcttg agctgccatt tgtgctgcat aaggagtgct ttttttagaa cctctgaaac
     2401 cacatttacc tgcactactc caagagatta cttgacctgc tttgttcgtg taagaaacga
     2461 tgatattatt aaaggtagcc tttatataca catagccttc tgcttctact tttactacgc
     2521 gttttttcgc tgttttttta gttgtttgtg ccattttata tgagcttcaa atttttacta
     2581 tttagttact ttctttttac ctgcaactgt tttgcgctta ccttttctag tacgtgcatt
     2641 acgttttgtg ctttgaccac gaactggcaa tccttttctg tggcggatac ctctgtaaga
     2701 accaatatcc atcaaacgtt tgatgtttaa ttgtgtttca gagcgaagtt gtccttctac
     2761 tttgtattca gcagtaattg cgttacgaat agcggttgcc tcgtcgtcat tccactcatg
     2821 aacttttttc tgtggatcaa cacctgctgt gtttaaaatt gctaatgctg ttgatctgcc
     2881 gattccataa atataagtaa gaccaatgta gcctctttta ttttttggaa gatctatacc
     2941 tgatactctt gccatagtat tatccttgtc gttgtttaaa ttttggattc tttttattta
     3001 tcacatacac tttgcccttt ctgcgtacaa tgatgcagtc ttcgcttctt ttttttactg
     3061 atgcttttac cttcatggta gtctatcttt agtttattta tatctgaaag ttattcttcc
     3121 tctggagaga tcgtaaggag aaatttcaac tgcgactttg tcgcccggaa gaattcgtat
     3181 gtagttcata cgcattttgc cggatattgt agctaaaatt tcgtggccgt tttccaactt
     3241 aacgcgaaac attgcgttag aaagagcttc cgtgatcgta ccgtctaatt ttattaaatc
     3301 tgttttaccc ataattttcg gactgcaaat atcgtaataa ttatctgaaa tccttaatat
     3361 attctgactt ttttatcgct tcgaatatta aattatggtc actaagtatg tctgccttgc
     3421 ctcttttaac tgctaatgtg tgttcaaagt gagcagatgg ctttccatct ctagctatta
     3481 tcgtccactt atcggataat gttcttacct gataagttcc catatttatc attggttcaa
     3541 tggctaatac cattccctct tgcaacattg gtccttttcc acgtttgcca taatttggca
     3601 cttgtggatc ttcgtgcaaa tcttttccaa ttccatgacc aactaaatca cgtacaacac
     3661 cgtagttatg cgtattttca caatagtttt gaatagcaaa tccaatatcg cctattcggt
     3721 ttccggcaat ggcttgttcg attcctaaat acaaggaatt gaaggttacc tgtaataatt
     3781 ttttactttc tgcacttacc tctccaattg gaaatgtgaa ggcagaatcg ccatgaaaac
     3841 catctttata tacgccgcaa tctactgata aaacatcgcc ttctttgatt tctctatccg
     3901 aaggaaaacc gtgtaccact tgttcattta cagaaataca caaagatgct ggaaaaccac
     3961 catatccttt aaaggaaggt gttgcgccat tatctctaat aaattgctct gcaatttcat
     4021 caagtttggc ggttttgaca cctggtttta tatattctac caaaagcgcg tgcgtctggc
     4081 taactaacaa agaactcttt cttatatttt caatttcctc gggcgttttt aaatgaatca
     4141 tacgctctta aaaattaata atctgatgta gcaacgtttc ttcctcttat ttttgcgcct
     4201 gattttgtta aaccatcata atggcgactt aataaatgag agtttatttg ttgcatagta
     4261 tctaatacta cggatactaa aatcaataat gatgtaccac caaagaaata ggcaaactgg
     4321 ctatttacac ctaaaacatt tgcgaatgat ggcatgattg ctataaatcc taagaatata
     4381 gaaccaggca atgtaattct cgacaatact gtatcaatat attcagctgt attagctcct
     4441 ggttttacac ctggaatgaa accattattg cgttttaaat catcagccat ttgcacagga
     4501 ttcataatta aagccgtgta gaaatatgta aataatacaa ccattaagaa catgataata
     4561 ttataaggca atgacgtaaa atctgttaac tgtcttaaaa atccagaagc cgaactatct
     4621 gggaacattt gagctattgt gcctggtata aacaagaatg cttgtgcaaa gataattggc
     4681 attacaccag caatatttac ttttagcggt aagaaacctc tgcgttgtgt gtaggcatta
     4741 ttaccaccag acatttgttt tgcataactc aatggcactt tacgaattgc ttgaataagc
     4801 attatggttg ctataattac gaatgctaaa acgaccattt ccaaaacaaa catcaatata
     4861 ccgctaccac ctgcaactgc tctacctttt gcttctgccc aaaacgatgc aggtagtctt
     4921 gaaatgatat ttgccataat gattaaggaa gtaccattac ctaagccacg ttcttgaatt
     4981 tgttcaccta accacattac aaatacagaa cctgtgatta aaataatcat ggtcgatagt
     5041 gaaaataatg tagtagaaat ttctggtgat acgatatttt gagagtacaa gaatttcaca
     5101 taagcaaagc cttggaaagc tgtcactgct actgttccgt agcgtgtgta ttgtgtaatt
     5161 ttctttctac cgctttcgcc ttctttttgc aatttttgga aataaggcac tgctaatgtc
     5221 aataattgca taacaataga agtagagata tatggcatta tacctaatgc aaatacagat
     5281 gcttttgtga aagcaccacc tgcaaataag tcaaatattt gaaatatacc gctagcgcca
     5341 ccgctgctga ttttagtagg atctacgcct ggtaatacta caaaacatcc taagcgatag
     5401 atgaaaataa gacctaaggt taagaatatt ctttttctca ggtcttctat gctgaagata
     5461 tttttaagag tgtcgaaaaa ttgcttcata agcttattat gctataactg tggcagcacc
     5521 actatttttt tcaattgctt cttttgcttt atcactgaat gcatgtgcag aaactttcaa
     5581 tgcacctttt aattcgccat tcgctaacac tttaatttta tcgctagctt ttactaattt
     5641 aagtgttcta tacgtttcga aagtgaactc tgtgatgtta tttttttctg ctaatgcttg
     5701 taattctgcc aagttgattg ttctaaattc aactctgttt ggatttttaa atccgcgttt
     5761 tggcaaacgg cgttgtaatg gcatttgacc accttcaaaa cctctttttg atttataacc
     5821 agagcgagat ttcgcacctt tatgtccttt tccagctgta ccgccagagc cagaaccttg
     5881 tcctcttgct atacgtttgc gaccttttac ggcaccttta gcaggtttta aattatttaa
     5941 actgatgctc attattagat gttttctacc gttactaaat gttttacttt tgctaccata
     6001 cctaatactt gcggtgtagc ttctttttca acgcttctgt ttagcttttg gatacctaat
     6061 gctttgatgg tatctttttg acgttgactt ctgtcgattg tactcttaac ttgagtgatt
     6121 tttatcttcg ccatactatt atccgttaaa tacttttgtc aatttaatac ctctgtctct
     6181 tgcaactgta aaaggatctc taagcaatgt taatgcttgg attgttgctt taactacatt
     6241 gtgtgggttg gctgaaccta atgattttgc taacacgtcg ctgataccag cgctttctaa
     6301 aacggcacgc attgaacctc cagcgataac acctgtacca tgagatgctg gtttgattaa
     6361 tactttagcg ccgtcgaatt ctgctgcttg gtcgtgagga attgtacctt ttaagatagg
     6421 aattttcact aagtttttct tagcatcatc gatacctttt tggatagctt cttgcacctc
     6481 acgtgctttg cccaaaccat ggccaactgt tccattacca tcacctacta cgactagcgc
     6541 agagaagctg aatgtacgac caccttttgt aactttagca acacgttgaa tacctaccac
     6601 tttttctttt aattcgattt ctgtggctgt tctaacttta ttttctttta cttttgacat
     6661 attttatttt tttgtgtgac gtattagcgc caatacctgc ttattaaaat tgcaatccgt
     6721 tttctctggc agcatctgct aaagctttaa ttctgccgtg gtataaaaat ccatttctgt
     6781 cgaatataac gttagcgata tttgcatctt tagctttaga agcgatagat gcaccaacag
     6841 ctttagcttt ttccgtttta gtgccagttg atttttgaga tgatacagaa gcaattgttt
     6901 tgcctgttgc gtcgtcaatg atttgagcat agatttcgct attgcttctg aatacgcata
     6961 atcttggacg ttctgttgta ccactgattt ttttacggat tctaaattta attttttctc
     7021 tacgagataa tttattagtt actgccatga caataatttt tttattttat tggttacacg
     7081 tgatttggcg tgcctacctt tgattatttt ttacctgcag acttacctgc ttttttacgc
     7141 aatatttcgc cagtatattt aatacctttt cctttgtaag gttcaggttt tcttaagctt
     7201 cttaatttag cagctacttg accaattaat tgcttgtctg tgctttctaa gataatacga
     7261 ggcggttgac ctttttcaga aacagtagat acttttactt cttctggaat taatacgaaa
     7321 attgggtgag agaaacctat tgacaattct aataattgac cttggttact agctctaaaa
     7381 cctacaccta ctacatctaa tgatttttta tagccttcgc ttgcacctaa caccatgtta
     7441 tttaatagtg ctctgtataa accatgtaaa gctctatggc gtttttggtc tgttggacgt
     7501 tccaacacta cattgccatt ttctactttt acgatgatat ctctgtccac tttttctgac
     7561 aaagtacctt ttggtccttt tactgtaacg acgttattat ctgcaacgtt cacttcagtt
     7621 tttgccggta ttgatattgg taattttcct acgcgtgaca tgtctttttt attttaagat
     7681 actatgcaaa taatttcacc acctacattt aagtttttcg cttgtttatc tgtaagaata
     7741 cctcttgacg ttgataaaat tgctaaacct aaaccatttt ttactctagg aatacctgct
     7801 acgtgtgcat aacgacgtaa gcctggttta gagattctaa cgagctctct gattgctggc
     7861 tctcttgttt caggattgta tttcaatgca atcttgatag taccttgagg accgtcttct
     7921 tcgaatttga attttaggat ataaccgttt tcgtgtaata tttcggttaa ttttttcttc
     7981 atattagaag caggaatgtc aacgatggta tgaccagcga gttgtgcatt tctgattctt
     8041 gttagaaagt ctgctattgg atctgtaacc atttcttttt ttgtttagac cgcttagatg
     8101 tgagacttaa gtcgttgtcg gctcagtttc ccaccttcac ttatcattta ttaattaatt
     8161 tctttacttt attaccagct tgctttagat aatcctggaa tcataccgta gctagctaaa
     8221 tctctgaatt gaattcttga caatccaaag aagccgatgt aaccacgagg acgacctgtt
     8281 agtttacatc tttttctgta gcgtactggt gatgcatttt ttggaagttt atccaatgca
     8341 gtgtagtctc ctgctttttt taattcgtca cgttttgctt tgtatttgtt gattagtttc
     8401 aaacgctttg catcgcgtgc tttcattgat tcttttgcca tgcttatttt ttcttaaatg
     8461 gtaaaccaat ttcttttaat aatgaatatg cttcgtcatc agtatttgca gatgtcacga
     8521 aagtgatatc cataccgctg attttagaga ttttgtcgat atcaatttca gggaaaatga
     8581 tatgctcagt gatacctaag gtatagtttc cacgaccgtc gaaagattta tcgttgatgc
     8641 ctttgaagtc acgaacctgt ggtaatgcca cggccataag acgttctaaa aactcataca
     8701 ttctttcgcc gcgcaatgta acacgtacac cgattggtaa attttctctc aatttgaagt
     8761 tggagattgc ttttttagat ttcgtagaaa cagctttttg acctgtgatt ctagaaattt
     8821 catcgataga attatctatc aatttcttgt ctccaacggc agcgcctaca ccttgattga
     8881 ctacaatctt ggttaacttt ggcacttgca tcactgactt gtagttgtac ttcgttttta
     8941 acgctggtac tacttcttca gtatattttt tctttaatgt aggaatgtaa ctcattactt
     9001 aattatttct cctgttgatt ttgcaattct tactgatttt ccatcttcta cttttctgcc
     9061 tactttagta gcttttccgc ctactgaaag cattaagtta gagatgttga taggtgcttc
     9121 ttttttcacg ataccacctt gttgattttg agcatttggt tttgtgtgtt tagaaattat
     9181 tcttacacca gcaaccaatg ctgtgttttt agaaggaaat acttctaata ctttaccgct
     9241 tttaccacga tcttctccag tgataacgat aactgtatct ccttttttta ttttcaattt
     9301 cggcgcaaag cgcttttgtg ttgatgtact cataattgat atacgcttat atttttataa
     9361 tacttctgga gctaatgaga taattttcat gtagtcttta tctctcagtt cgcgcgcaac
     9421 aggtccgaag atacgtgtac cacgtggttc gtcgttattg ttcaacaata ctactgcatt
     9481 gtcgtcaaaa cgtatatacg ttccatcttt gcgtttaatt tctttttttg ttctaacaac
     9541 taccgctttg gatacttgtt tcttttttac gttaccgcct ggcatcgcat cttttacaga
     9601 tactactatt ttgtcgccga ttgatgcgta acgagctctt gtacctttta atactctgat
     9661 gcaaagtact tctttggcgc cgctgttatc agctactcgt agtcttgatt cttgttgtac
     9721 catcttattt agctttttct acgatttcta ctaatctcca tctttttgtt gcacttaaag
     9781 gacgtgtttc cataattttt acggtatcgc ctatattgca ttcatttttt tcatcatgtg
     9841 catgaaattt cttagttttc tttaagaatt ttccgtactt aggatgtttt aatttacgaa
     9901 caacagctac agtaatagtt ttttccattt tattggaggt tactataccc actcttgttt
     9961 ttcttaattg tctttccgtt gttaactcgg ttgtcatctg cacttagttt atgcttgttt
    10021 agataatttt ctttttctca attccgtttg gatacgagcg atgtgtttgc gtgtttcgcc
    10081 taaaacattc gtattttcca atggtgtgat agcgtgattg aatttcaatt ttgtcagttt
    10141 caattttgaa gcggacaact cgttgttcaa ttcttcatca ctccaagatg ttatttcctt
    10201 atatgctact gttgttgcca tttttttaga ttttattgat tagcggcgtc gtaatctctt
    10261 cttacgataa atttggttaa taccggcaat ttttgagctg ccaatcgtaa tgattcttct
    10321 gccaattctt tagacacgcc ttcgatttca aataagattc tgcctggttt tacaacagcc
    10381 acccaatgat ctaatgaacc tttaccttta cccatacgta cttcagctgg ttttttagtg
    10441 attggtttat ctggaaatat gttaatccat actttacctt cacgtttcat atttcttgtt
    10501 aaagcaatac gcgcagcttc aatttgacgg tctgtaatcc aacattcgtc taatgatttc
    10561 aatccgaatg aaccgaatga caattgcgat cctttgtacg caatgccttt cattttcatt
    10621 ttgtgaactc ttctaaattt tactcttttc ggttgtaaca
//
LOCUS       DAOQQV010000089        10450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2320_length_10450_cov_6.661953, whole genome shotgun sequence.
ACCESSION   DAOQQV010000089 DAOQQV010000000
VERSION     DAOQQV010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2320_length_10450_cov_6.661953"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLL59_09265"
     CDS             complement(<1..391)
                     /locus_tag="PLL59_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMY24039.1"
                     /translation="MKQLKITTKITSRDSFSIDKYLSEISKLPMLSEEEEVKVAQQIR
                     AGDTEAFEKLVQSNLRFVVSVAKQYQNQGLALNDLINEGNIGLIKAASKFDETKGIKF
                     ISYAVWWIRQSILQALIEQPRVVRLPMN"
     gene            complement(630..2750)
                     /gene="pnp"
                     /locus_tag="PLL59_09270"
     CDS             complement(630..2750)
                     /gene="pnp"
                     /locus_tag="PLL59_09270"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HMY24040.1"
                     /translation="MQFNIHTQTVDIGNGRTATIETGKMARQADGAVVVRVGNCMLLA
                     TAVASEKPKDGVDFFPLTVEYQEKFAAAGRIPGSFHRREGRASDYEVLICRIVDRAIR
                     PMFADNFVNDTQVLIYLISADENVEPDSLVGLAASAALSISSIPFEGPISEVRVTKIG
                     DNYVVNPTPKEAENATLNIILAGTVDNILMVEGEAKEASEAELIAAIKAGHEIIIKQC
                     EAQVALQQKCGKPKMEVVAPIEDEVLYNKVKEFVVERIETIAQSALGKHERKGLLKLI
                     KEDFIANYPEEDLEIFNQELFNKYYSKIEKNTIRKVVLDKKVRLDGRKTDEIRPLIME
                     VDVLPSPHGSALFTRGETQSLTTVTLGSKTDEQMIDKAEELSYNRFMLHYNFPPFSTG
                     EVKMLRGTSRREVGHGNLAMRSLKQLLPSEDENPYTIRVVSDILESNGSSSMATVCAG
                     SLALMDAGIPFKKHVSGIAMGMIAEDGKYAILSDILGDEDHLGDMDFKVTGTKDGICG
                     VQMDLKVDGLSYEVLAEALEQARKGRLHILAAMEQCIPSYRPDLKPQTPRAESINIPK
                     EFIGAVIGPGGKIIQDIQEKTSTVITIEENKEKGIGKVTVLSNNKENMTAAWARINAI
                     VAVPEIGNTYDAEVKSIMPYGAFVEFMPGKQGLLHISEIAHARLESLDGVLAEGDKVK
                     VKLLEIDTKTGKFKLSKKALLPKE"
     gene            complement(2815..3078)
                     /gene="rpsO"
                     /locus_tag="PLL59_09275"
     CDS             complement(2815..3078)
                     /gene="rpsO"
                     /locus_tag="PLL59_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HMY24041.1"
                     /translation="MTTTEKKAIFTEFGGAATNTGSVESQVALISHRINHISNHLKTN
                     RKDFSSTRSLYKLVGQRKRLLKYLANKDIVQYRALIEKLGLRK"
     gene            complement(3229..4749)
                     /locus_tag="PLL59_09280"
     CDS             complement(3229..4749)
                     /locus_tag="PLL59_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMY24042.1"
                     /translation="MQFLKNLGIEAVNTGSSTGITWISTHEKIDSISPVDGKIIASVQ
                     ITNKETYEKVLDKATSACIEWRNVPAPKRGEIIRQMGNEFRIHKQDLGMLVSWEMGKS
                     LQEGLGEVQEIIDICDFAVGLSRQLYGLSMHSERPKHRMYEQWHPLGIVGIITAFNFP
                     AAVWGWNAMLAWVCGNVCVWKPSEKTPLTAIACQKIVEKVIRENELPEGLSCLINGDK
                     TVGEWIATDERISLVSATGSTRMGKEVGKMVASRLGRTLLELGGNNAIIITPNADLKL
                     AVIGATFGATGTTGQRCTTTRRLIIHSSVYEKFKEQLITAYQQLKIGNPLEDGVHVGP
                     LIDNQAVENYLAAIKSCEEQGGNFIVKGEKINVNNYESGFYAKPCIAEVPSQLPIVLT
                     ETFAPILYLLKYEKLEEAIALQNEVPQGLSSAIMTTNMREMEAFLSASGSDCGIANVN
                     MGTSGAEIGGAFGGEKETGGGRESGSDAWKSYMRRQTSTINFGLDLPLAQGIKFDI"
     gene            4889..5230
                     /gene="yajC"
                     /locus_tag="PLL59_09285"
     CDS             4889..5230
                     /gene="yajC"
                     /locus_tag="PLL59_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HMY24043.1"
                     /translation="MSLLFLLQANAGAPGGGMSTIIMMGLMFIVFYFFLIRPQSKKAK
                     EAQNFIETLKSGDKVITNSGIHGKIVSVNDEDKTFLIQVDGPTRLKMEKSAINAELSK
                     VINATPSVEVK"
     gene            5260..5847
                     /gene="coaE"
                     /locus_tag="PLL59_09290"
     CDS             5260..5847
                     /gene="coaE"
                     /locus_tag="PLL59_09290"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HMY24044.1"
                     /translation="MLKIGITGGIGSGKTTVCKIFETLGVPVYYADTRAKELMLTDNS
                     LIENIKSLFGNEAYTDNGELNRKFIASKAFQDKSILQKLNQIVHPAVFQDTIHWYALH
                     NDKPYVLYEAAIMFETGSYTLFDKIIVVAATLEDRISRTMLRDDLSREEVLERISKQI
                     PDEEKIARADFVIYNDYSQPLIQQVLTIHNNILAI"
     gene            5915..6847
                     /gene="trxB"
                     /locus_tag="PLL59_09295"
     CDS             5915..6847
                     /gene="trxB"
                     /locus_tag="PLL59_09295"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HMY24045.1"
                     /translation="MIETVNCLIIGAGPAGYTAAIYAARANLKPVLYTGLQPGGQLMT
                     TTDVENYPGYPDAVTGPKMMDDFQKQAERVGADIRFGMATKVDFSGDIHKVWIDEETE
                     IHAKAVIIATGAEAKWLGIPSEKRLNGSGVSACAVCDGFLFKGKVVAIVGAGDTACEE
                     ALYLSNLCPTVHMIVRSDKMRASKIMQDRVLAKDNIIVYWNSETDEVLGGNKVEAVRL
                     KNNKTNEITEIPIEGFFVAIGHKPNSDIVKGWIDMDDTGYITTIAGSTKTNIEGVFAA
                     GDVQDKIYRQAVTSAGSGCMAALDAERYLAEKFH"
     gene            complement(7010..7648)
                     /locus_tag="PLL59_09300"
     CDS             complement(7010..7648)
                     /locus_tag="PLL59_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMY24046.1"
                     /translation="MKKHKIILVDDHQMLIDGLSAILANNDEFEIIKTFTNGNKLLQE
                     IDTLNPDIVLTDISMPEIDGYELTKKIKAYNDNIHVIALSMSGSIANINKMLDVGISG
                     YVLKNVGNTELTEAIHKVIQGKMHFSDEVMEEMVKNKHTKQEKQREQTKFTERELEIL
                     KLIIEEFNNAEIADKLFISERTVETHRKNMIRKFNTKTIVGLIKYAMDNKLV"
     gene            complement(7664..9547)
                     /locus_tag="PLL59_09305"
     CDS             complement(7664..9547)
                     /locus_tag="PLL59_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMY24047.1"
                     /translation="MKFLFIICIFCFIACTNSTPSLLLNNEKQIDYFDAKYQENQRVN
                     VVVAQNYIDSLLQFSIEKNSIKGVAIATLNRGILENIKGNFNLAIQQNEKALALFKEL
                     KEDTLTAKALQALGVNFWQKGENDKALQLFLESFDINEKLQLVNEMASNYNSISMIYQ
                     SKHNIQLAEEYANRAMDLIKGNTPAKSHISILHNMANIYGMQEKYQAALRLDSIGLAY
                     CNQLNIQFNKSMFYDNIANCYFFMKQYDTSIYYHTKAISIDSSFGNKKQLGDTYNNLG
                     YIYAVLNDVDKAEFYYLQSIQLCKETGYKIGIQNALSHLSNLYYTHQKPNEAYELLSE
                     SISVKDSIISDASEQKIAELQTLFETEKKKKKIAEQDLKISRRNILLITLFSILVLSL
                     ITYYLFYNRYKSKQEQKLQQEQLKSELKRTQAILESEEKERQRLARELHDGVGQLLSA
                     AKLNLSAIQTAELKSAGQIVNSMKIIDDSITEIRSISHNMVPDALQKNGLVFAVDNFI
                     EKLNQLKEQEISFEYYGYVENMLDDAAKLMLYRIIQEAVNNAIKYAEASTIAVQLSAD
                     EHEISLIIEDNGKGFDIENIKSKNGIGLRNMQLRTDYLHGKLNIESSEKNGTTLIVEI
                     PLS"
     gene            9695..10339
                     /locus_tag="PLL59_09310"
     CDS             9695..10339
                     /locus_tag="PLL59_09310"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HMY24048.1"
                     /translation="MKIISFFFFVCIFSFSVHAQSERDTIVLPQMIIDGDTLEEIILE
                     PILVTASKLEKVDLQEWQMRYLRKVYPYALRTARLTKKIDEDLQNCKTKKERKRYLNE
                     CEKVLRAEFETTLRNMSRTQGKYLIKLINRETGQTVFELLKEYRSNWKAFWWNFAGKF
                     FDLDLKAEYDPSGEDKQIENYIQRLQYIYHTDGTNYLIQHEQFHTPIPGEKRSK"
     gene            10293..>10450
                     /locus_tag="PLL59_09315"
     CDS             10293..>10450
                     /locus_tag="PLL59_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMY24049.1"
                     /translation="MNNSIHQFQAKNAQNKVVDFADYKGKVLLVVNTASKCGLTPQFE
                     QLEALYQE"
BASE COUNT         3225 a         1774 c         1743 g         3708 t
ORIGIN      
        1 tattcatcgg caagcgcacc acgcgtggtt gctcgattaa tgcttgcaaa atagactgac
       61 gaatccacca aactgcataa gaaataaatt taatgccttt cgtttcatca aatttgctag
      121 cagcttttat taatccgatg tttccttcat taattaagtc gtttaatgcc aagccttgat
      181 tttggtactg cttcgctacg gaaacgacaa atcgcaaatt tgattgcact agtttttcaa
      241 aagcctctgt gtcgccggct ctaatttgtt gtgccacctt tacttcttct tcttcagaaa
      301 gcataggtaa ttttgagatt tcactcaaat acttgtcgat ggagaaactg tctcgcgacg
      361 tgattttggt ggtaattttc agttgtttca tgtgtcttta cagtgttagg ttaaataaat
      421 tcacatgcta caaagcaaca gtaccaaaaa ttaagaatag atttttgttg tacaaatttg
      481 tcataagcag cataatagtt catactaata tacgattttg ttaaaaaaat gttacaaaac
      541 gtattaatat ttttttgcac atcaaaaata aacataaaaa aaccgacaat taagattgtc
      601 ggtttatgtg ttgtgacatg ctagaagcat tattctttag gaagtaaagc ttttttgcta
      661 agcttaaatt tacctgtttt tgtatcaatt tctagcaatt ttacttttac tttatcgcct
      721 tcagctaata cgccatctaa agactctaag cgagcgtgtg cgatttcaga aatatgtaat
      781 aatccttgtt tccctggcat aaattctaca aatgcaccat atggcataat agattttact
      841 tcggcgtcat aggtgtttcc aatttcaggc actgcaacaa ttgcgttgat gcgtgcccaa
      901 gccgctgtca tattttcttt gttattagac aaaacagtta ctttaccgat tcctttttct
      961 ttattctctt cgatagtgat aactgtactt gttttctctt gaatatcttg gatgatcttt
     1021 ccgccaggtc caattactgc gccaataaat tcttttggaa tattaatgct ttcagctctt
     1081 ggtgtttgtg gtttaagatc tggtctatat gatggaatac attgttccat agctgctaag
     1141 atatgtaagc gaccttttct tgcttgctct aatgcttctg ctaatacttc gtatgataaa
     1201 ccatcaactt ttaaatccat ttgaacgccg caaataccat ctttagtacc tgtaacctta
     1261 aagtccatat cacctaaatg atcttcatct cctaagatat cagataagat agcatattta
     1321 ccatcttctg caatcatgcc catggcaata ccagaaacgt gttttttgaa aggaatacca
     1381 gcatccatta aggctaatga accagcacaa acagttgcca ttgatgagga accattcgat
     1441 tctaaaatgt cagatacaac acgaattgta tatggatttt cgtcttcact tggcaataat
     1501 tgttttaaag aacgcatagc taaatttccg tgaccaactt cacgacgaga agtacctctt
     1561 aacattttta cttcgccagt agaaaaagga ggaaagttgt aatgcaacat aaatctgttg
     1621 taagataact cttccgcttt gtctatcatt tgttcatcgg ttttagaacc taatgtaacg
     1681 gtggttaatg attgcgtttc gccacgtgta aataaagctg agccatgagg tgatggtagt
     1741 acatcaactt ccataattaa tggacgaatt tcatctgttt ttctaccatc taaacgaact
     1801 ttcttatcaa gtactacttt gcgaatagta tttttctcta ttttgctata atatttatta
     1861 aacaattctt gattgaagat ttctaaatct tcttcaggat agttagcaat aaaatcttct
     1921 ttgataagtt ttaataagcc tttacgttcg tgtttgccta aggcagattg tgctatcgtt
     1981 tctatgcgtt ctaccacaaa ttcttttact ttgttataaa gtacttcatc ttcgatagga
     2041 gcaacgactt ccattttagg cttgccacat ttttgttgca atgctacttg cgcttcacac
     2101 tgtttaataa tgatttcgtg acctgctttg atggctgcaa tcaactcagc ttcgctagct
     2161 tctttagctt cgccttccac cattaaaatg ttgtcaaccg taccagctaa gatgatattt
     2221 aatgtagcat tttccgcttc tttaggtgtt ggatttacta cgtagttgtc accaatcttc
     2281 gtcactctta cttcagagat aggtccttcg aatggaatgg aagaaataga aagcgcagca
     2341 gaagcagcta aacccaccaa agaatctggt tctacatttt cgtcagcaga aattagatat
     2401 attaaaacct gagtgtcgtt tacaaaatta tcggcaaaca tcggacgaat agctctgtcc
     2461 acaatacggc aaataagcac ttcgtaatcg cttgctctac cttctctgcg atgaaagcta
     2521 cctggaattc taccagcagc agcaaatttt tcttgatatt ctactgttaa aggaaaaaaa
     2581 tcaacgccat cttttggctt ttccgacgct acagcagttg ccaataacat gcagttgcct
     2641 acgcgtacaa ctactgcgcc atctgcttga cgagccattt ttccagtttc aatagtagca
     2701 gttctgccgt ttcctatatc tactgtttga gtatgtatat taaattgcat aatattttat
     2761 taaattgtat taagaataaa aaagcgaggc tgaagcctcg ccaataaatg taaattactt
     2821 acgtaatccg agtttttcga ttaaagctct atattgtacg atatctttgt ttgcaagata
     2881 ttttagtaat ctttttcttt gtcctactaa tttgtataat gaacgagtag aagagaagtc
     2941 ttttctgttc gttttcaaat ggtttgaaat gtgattgatt ctgtgagaaa tcaatgcaac
     3001 ttgagattct acagatcctg tgtttgtagc tgcaccgcca aactctgtga aaattgcttt
     3061 tttttctgtt gttgtcatct tttttgtcgt ctatttgttt agattctatt tattttcgaa
     3121 gtgcaaagat acacatttat aatgttatta caaaattatt attgacatat tgtggattac
     3181 gccaccctca ggtgattgat gaatagttca ttttttgttt tgcttctgct aaatgtcaaa
     3241 ttttatacct tgtgctaatg gcaaatctaa tccaaaattt atcgtgctgg tttgtcgacg
     3301 catatatgac ttccacgcat cagaccctga ttcacggcct ccacctgttt ctttttcgcc
     3361 accaaatgcg ccaccaattt cagcaccaga agtacccata tttacattag ctattccaca
     3421 atctgagcct gacgccgaaa gaaatgcttc catttcacgc atatttgttg tcataatggc
     3481 tgaagataag ccttgtggta cctcattttg taatgcaata gcttcttcta gtttttcata
     3541 ttttagtaag tataaaatag gagcaaaagt ctctgtaagt acgattggta gctgagatgg
     3601 aacttctgcg atacaaggtt ttgcataaaa accagattca taattattta catttatttt
     3661 ttcacctttt actataaagt taccaccttg ttcttcacat gattttattg cagctaagta
     3721 attttcaact gcttgattat caattaatgg tccaacatgg acaccgtctt ctaatggatt
     3781 ccctattttt aattgttgat atgctgtaat tagctgttct ttaaatttct catatacaga
     3841 tgaatgtatt attaatcttc tagtagttgt acatctttga ccagtagttc cagtggcgcc
     3901 aaaagtagct ccaataacgg ctaattttaa atcggcattt ggtgtgataa tgatggcatt
     3961 atttccacct aattccaata gtgttctacc taatcgagat gcgaccattt tgccgacttc
     4021 ttttcccatt ctagttgagc ctgttgcaga aactaaggaa atgcgctcgt ccgttgcaat
     4081 ccattcaccg acagttttat ctccattaat taagcaagac aatccttctg gaagttcatt
     4141 ttcgcgtatt actttttcaa ctattttttg acaagcaatt gcggtcagcg gtgttttctc
     4201 tgatggtttc catacgcaaa cattgccaca aacccaagcc aacatagcat tccagcccca
     4261 aaccgccgca ggaaagttaa aagctgtaat aataccgaca attcctaaag gatgccattg
     4321 ctcatacatt ctgtgttttg gccgctctga atgcatactt aggccatata attgacgtga
     4381 taaaccgact gcaaaatcgc aaatgtcaat tatttcttgt acttcaccta gtccttcttg
     4441 taggcttttt cccatttccc aagaaaccaa catgcctaaa tcttgtttgt gaatgcggaa
     4501 ttcattgccc atttgacgaa tgatttcacc acgttttggc gctggaacat ttcgccattc
     4561 aatacaagca gaagttgctt tatcgagtac tttttcgtat gtttctttat ttgtaatttg
     4621 tacagaagcg atgattttgc catctacagg cgaaatggaa tcaatttttt catgggtact
     4681 tatccaagta ataccagtgg aagatcctgt atttactgct tctataccga gatttttgag
     4741 aaattgcatt gtttatattt tgatacttaa gattgatttt tgaagaaaaa gaatgacatt
     4801 ttcaataaag ttactaaaat gttttatctt ttattttatc taatacctat ttaaaaatag
     4861 gtaaatttgt tgtttaaaat tatcaaaaat gagtttatta tttttactac aagcaaatgc
     4921 tggtgcacca ggcggtggaa tgtcaacgat tatcatgatg ggtttgatgt ttatcgtatt
     4981 ttatttcttc ttaattagac cgcaatctaa aaaagcaaaa gaagctcaaa attttattga
     5041 aacattaaaa tcaggtgata aagtaattac taattcagga attcatggca agatagtttc
     5101 tgttaatgat gaagacaaaa cattcttaat acaagttgat ggaccaactc gtttgaaaat
     5161 ggaaaaatct gccattaatg ctgaactgtc gaaagttatc aatgcaacgc caagtgtaga
     5221 ggtaaaataa gattcgatac tatttgctta ttatgtttta tgttgaaaat tggcatcaca
     5281 ggtggcattg gtagtggaaa aactacggtt tgtaaaatat ttgagacgtt aggtgtacct
     5341 gtgtattatg ctgatacacg agcgaaagag ttgatgctta cagacaatag tcttattgaa
     5401 aacatcaaat ctttatttgg taatgaagca tacacggata atggcgaatt gaataggaaa
     5461 tttattgctt ctaaagcatt tcaagataaa agcattctac aaaaattaaa tcagattgtt
     5521 catccagcag tttttcaaga tactatacat tggtatgcat tgcacaatga caaaccatat
     5581 gttttatatg aagctgccat catgtttgag acaggaagtt atacgctttt cgacaaaata
     5641 atcgtagtag ctgctacttt agaagataga attagccgaa ctatgctgcg cgatgactta
     5701 agtagagaag aggtactcga gcgtatatcg aaacaaattc ctgatgaaga aaaaatagct
     5761 cgtgctgatt ttgttatata caatgattat tctcaaccat taatacaaca agtgttgaca
     5821 attcataata atattttagc aatataaaag cagaactatt tatttttgcg cattgttatt
     5881 aaacaaagtt attctcaatt tataattatt ggatatgata gaaaccgtta attgcttaat
     5941 cataggagct ggtcctgctg gttatactgc agccatttac gctgcacgtg caaatttaaa
     6001 accagtatta tatacaggct tgcaaccagg cggacagttg atgacaacta ctgatgttga
     6061 aaattatcct ggctatcctg atgcagtaac tggaccgaaa atgatggatg attttcaaaa
     6121 acaagctgaa agagtaggtg ctgacatacg ttttggtatg gctacgaaag tagatttttc
     6181 tggcgacata cataaagtgt ggattgatga agaaacagaa atacatgcca aagctgtaat
     6241 tatcgcaaca ggagcagaag cgaaatggct tggtattcct tctgaaaaaa ggctaaacgg
     6301 tagcggtgtt tctgcttgtg cagtttgtga tggattttta tttaaaggaa aagtagttgc
     6361 tattgttggc gctggtgaca ctgcttgtga ggaagcatta tacttatcta atctttgtcc
     6421 gacagttcac atgattgtac gtagcgacaa aatgcgtgct tcaaaaatta tgcaagaccg
     6481 tgttttggca aaagataaca ttattgttta ctggaacagc gagaccgatg aagtgcttgg
     6541 cggcaataaa gttgaagctg tgcgattgaa aaacaataaa acaaatgaaa taacagaaat
     6601 tccaattgaa ggtttttttg tggccattgg tcataagcca aattctgata tcgtaaaagg
     6661 ctggatagac atggatgaca ctggttatat tactacaatt gcaggttcga caaaaacaaa
     6721 cattgaaggt gtttttgctg ctggcgatgt acaagataag atatacagac aagcggtaac
     6781 atctgcaggc agtggttgca tggcggcctt agatgcagag cgttatttag cagaaaaatt
     6841 tcattaaaca atgaagcatg aaatataaat ataaaacgga gttctttgga actccgtttt
     6901 tattttatgg tttttgtcca taaataatta tagtcaaaac taagcttata aagtcttttt
     6961 ggatactagt attatgtggt atttgttact acgtgctagt acgtagtatt taaactaatt
     7021 tattgtccat cgcatatttg ataagaccga cgatagtttt ggtattgaat tttctaatca
     7081 tatttttgcg atgcgtttcc acggtacgct cactgataaa tagtttatct gcaatttcag
     7141 cattattaaa ttcttcaata attagtttta aaatttcgag ttcgcgttct gtaaatttag
     7201 tttgttctcg ctgtttttct tgtttggtgt gtttgttttt taccatttct tccatcactt
     7261 catcgctaaa atgcattttt ccttggataa ctttatgaat ggcttctgta agctcggtat
     7321 tacctacatt tttcaaaaca taacctgata taccaacatc aagcatttta ttgatattag
     7381 caatgctacc agacatagat aatgcaatga catggatatt gtcattatat gctttaattt
     7441 tttttgttaa ttcatatcca tcaatttcag gcatgctaat atctgtcaat acgatgtctg
     7501 gatttagcgt gtcaatttct tgtaataatt tattaccatt ggtgaaagtt ttaataattt
     7561 caaattcgtc attattggct aaaatggctg ataagccatc aatcaacatt tggtgatcat
     7621 ctactaaaat tattttgtgt tttttcattt tttttgaatg ccgttaagat agtggaattt
     7681 ccactattaa agtagtacca tttttttctg atgattcaat atttaatttt ccatgtaaat
     7741 aatctgttcg caattgcatg tttcttaatc caataccatt tttagacttt atattttcta
     7801 tatcaaatcc tttgccatta tcttcaatta ttaaagaaat ttcatgttca tcagcagaga
     7861 gctgtacagc aattgtgctt gcctcagcat atttaattgc attatttact gcttcctgaa
     7921 taattctata taacattagc tttgctgcat catctaacat attttctacg tatccataat
     7981 attcaaaaga aatttcttgt tcttttaatt gatttaactt ttcaatgaag ttatctacag
     8041 cgaaaactag tccattcttt tgcaatgcat caggcaccat attgtgtgaa atgcttctta
     8101 tttctgtaat tgaatcgtcg atgattttca tcgaattaac aatttgtcca gcacttttta
     8161 gttcagcagt ttgaattgca ctcaagttta attttgcagc agataataat tgtccaacgc
     8221 catcatgtaa ttcgcgtgca agtctttgcc tttctttctc ttcagattct aaaatggctt
     8281 gcgtacgttt gagttcgctt tttaattgtt cttgttgtag tttttgttct tgttttgatt
     8341 tgtacctatt ataaaataaa tagtaagtaa ttaatgataa caccaaaatt gaaaataagg
     8401 tgatgagtaa aatattcctc ctgctgattt ttaggtcttg ctctgcaatt tttttcttct
     8461 ttttttctgt ttcgaataag gtttgcaatt ccgcaatttt ttgttctgat gcatcactta
     8521 taatactatc ttttacgctt atgctttcac ttaataattc gtatgcttca ttaggttttt
     8581 gatgtgtgta atataaatta cttaaatgag ataatgcatt ttgaatgcca attttatagc
     8641 ctgtttcttt gcacaattga atactttgca aataataaaa ttctgcttta tctacatcat
     8701 ttagaactgc atatatgtaa ccgagattgt tatacgtgtc gcctaattgt tttttattgc
     8761 caaaagaaga gtcaattgaa attgctttag tatgataata aatagaagta tcatattgtt
     8821 tcataaaaaa ataacaattt gcgatattgt cataaaacat tgatttatta aattgaatat
     8881 ttagttgatt gcaataggct aaaccaatac tatctaatcg caaggcggct tgatattttt
     8941 cttgcatgcc gtatatattt gccatattgt gtagaataga aatatgcgat tttgctggcg
     9001 tatttccttt tatcaaatcc atggctctgt tggcatattc ttccgccaat tgaatattgt
     9061 gtttagattg atatatcata gaaatcgaat tataattcga tgccatttca ttgactaatt
     9121 gtaatttttc attaatatca aacgactcta aaaaaagttg taaagctttg tcgttttcgc
     9181 ctttttgcca aaaatttaca ccaagtgcct gcaatgcttt tgctgttagt gtatcttcct
     9241 ttaattcttt gaataatgct aaagcttttt cattttgttg aatagccaaa ttgaaattgc
     9301 cttttatatt ttccaatatt cctctgttta atgtagcaat agccacacct tttatagagt
     9361 tcttttcaat ggaaaattgc aaaagcgaat cgatataatt ttgagcaact actacattta
     9421 cacgttggtt ttcttgatac ttagcatcga aataatcgat ttgcttctca ttgtttaata
     9481 ggagagaagg agtactgtta gtacaagcaa taaaacaaaa aatacatatt ataaacagga
     9541 atttcatgtt accacataga aatattatat agtttagcta ttttacgtta aaaatgaatg
     9601 aaaacaaaat aattagttca attttatgtt gaaatttaat gaatcttttt ggaacaataa
     9661 ttgtttattc taaatgtaat tataaacaga tacagtgaaa ataattagct tcttcttttt
     9721 tgtatgcatt ttttcttttt ctgttcatgc acaatcagaa agagatacaa ttgttttgcc
     9781 acaaatgata attgatggcg acacgctaga agagattatt ttagaaccaa tattagtaac
     9841 agcatctaag ttagagaaag tagatttgca agaatggcaa atgcgatatt tgcgtaaagt
     9901 gtatccatat gcattgcgaa ccgcacgctt gacaaaaaaa attgatgaag atttgcaaaa
     9961 ttgcaaaact aaaaaagagc gaaaacgtta tttgaatgaa tgtgaaaagg ttttaagagc
    10021 tgagtttgaa acaacattga ggaatatgtc gcgcacacaa ggcaaatatt taatcaaact
    10081 gattaatcga gaaactggac aaaccgtttt tgaattatta aaagaatatc gtagcaattg
    10141 gaaagctttt tggtggaatt ttgctggtaa gtttttcgac ttagatttaa aagctgaata
    10201 tgatccaagc ggagaggaca aacaaatcga aaattacata caacgtttac aatatattta
    10261 tcacactgac ggtacaaatt atttgataca acatgaacaa ttccatacac caattccagg
    10321 cgaaaaacgc tcaaaataaa gtagtcgatt ttgctgacta taaaggcaaa gttttattag
    10381 tagtcaatac tgcatcgaaa tgtggattaa cacctcaatt tgagcaattg gaagcgctat
    10441 atcaagaatt
//
LOCUS       DAOQQV010000090        10405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2337_length_10405_cov_7.590338, whole genome shotgun sequence.
ACCESSION   DAOQQV010000090 DAOQQV010000000
VERSION     DAOQQV010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2337_length_10405_cov_7.590338"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLL59_09320"
     CDS             <1..612
                     /locus_tag="PLL59_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY24050.1"
                     /translation="AADDAQALAAELNGDSYLLDISQATAAKELQKYIIEKYGKLDIL
                     VNNAGITRDKTIAKMSIDQWRSVLNVNLKAVIQLTQIFIENGFSKDAKVVSLSSISGI
                     SGNVGQTNYSLTKAGVIGFSQALAATNKNIFANAVAPGFIETKMTENLPFFIKEGGRR
                     LSTLKQGGKPEDVAELIGFLASPLSNGINGQCVRVCGGNMIGA"
     gene            752..2005
                     /locus_tag="PLL59_09325"
     CDS             752..2005
                     /locus_tag="PLL59_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24051.1"
                     /translation="MKRLFITGLLAVLTLSIFAQSTTTSNETVKLKIRKKVDRPTDKY
                     WYMTLSGGYGIPFLATNKRSPLKEVGDKQWYQHGNDLSVKSKFGTNGGGFAFNVGWGH
                     MFNKYIGIDVLHTFAWHPESLAAKIDLDTYYATQKTGTFSIYVSPHLVMRWDNGKRFG
                     ITGKAGLVLPIFGKTTSRAYILDKQGRVIETLAGLPILPLSIPGLLGLEIEIVGKAHT
                     EYHPTVGVSASIGFDVKLNKQLSLFAEMRVQAYTISLRETIFDEFKQTSKINVLGIKL
                     DAPEGMLAIPSNVANVSEAPEFLKHYVYRKELNENSNTARYGTRSLFAEDGLGGLVRT
                     LTGQTGLPSVDINKPMEEPGQKFNASSLYFNAGLRVNFDHWNKKRSSKRVAQASKSSS
                     KPSKSKKSKSSKSMVDDPDSTINVQ"
     gene            2018..3214
                     /locus_tag="PLL59_09330"
     CDS             2018..3214
                     /locus_tag="PLL59_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24052.1"
                     /translation="MKRNILIISAILLFAAPVFAQKKKAKTMSLSSTVGDKKGDVSES
                     KLKIRKNVTRPEGKGWFMYGSAGYGIPFLSTNKFSPFKEIGNKDWYQTPNSLNVKSIF
                     GTLGGGWQASIGWGHMFNKYIGIDILHTLSFHAEQLDARINTDIDLGGGKRASYFAEE
                     YTKIMPAIYLNPHLVMHWDNGKRFGVTGKAGLCMPMGGTPVTRAKIIDKSGRLVQTLA
                     GLPVIPLTALADFSSEYTATAKSKLKPTIGLSASIAFDVRLFKNVWAFAEIRVQAFTI
                     SPKETEFTEFSLKTESPLLPIISALTPLPLNVANINETPVYLKHFVYVKEITEESNTM
                     RYSSGLSLKEIDMNKPMEEPAVKYNASTLYFNAGVRMNFTQNWEKRRAAKVIPKREAK
                     AAKKNK"
     gene            3285..4448
                     /locus_tag="PLL59_09335"
     CDS             3285..4448
                     /locus_tag="PLL59_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY24053.1"
                     /translation="MKIKWTILGCLLLIQSSIVFAGFTTGTFKYKGAWRKYAIYVPAI
                     YASSSEKVPLLLGLHGYGDDIDNFRNICMTNIADTANYICVYAEALPWLGANAWNSGA
                     GVGIINVNSEVDDVGFLNSLIDTVISKYRIDTTRMYAFGFSFGGFMVDRLATECSQRF
                     AAACNVSGLHGNFLAGKIPSTYVPYLKFHGTNDPTISYNGMQTVGLFPGFGLSAENTV
                     KFWMTQNHCDTIPIVDSMPNTANDGLRFIRFTYNNDYNNSKVIFYKVLNGLHKWYGLP
                     ANDISYCQTIWDFFRQYKRENFVTAIRNINHVEQIKIFPNPSNGNFTVSLQNLNYNIQ
                     QISIFDIAGKEVYTQAVTTQSPITIVSNLPKGLYLLRLVDNEKHAMTEKIIIE"
     gene            complement(4459..5280)
                     /locus_tag="PLL59_09340"
     CDS             complement(4459..5280)
                     /locus_tag="PLL59_09340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY24054.1"
                     /translation="MSTKIKVKLGRVQETLLLPLVSRAKETEYKNPLLNDTKAVGLFK
                     QIDVDKKKLLRNITEIGVHGLCYRAYKMDEAIKAFLQKHPNGKILDIGAGLDTTYYRC
                     DNGKALWYDLDLPDSIALRTQLLPPPNKRVNYIAKSMLDYSWIDDIGDISNGLFITIP
                     GVLPYFKEEDVRVFLSTIAPRLKGAEIMFDVISQLGKFFVDMRIKAAGMKEAHLEWGI
                     LDAKTVTDWSKHIELVRTIKFFEAMPKSNQRLSTKVAMQFNRFFDMTQLFHLRFV"
     gene            complement(5321..7402)
                     /locus_tag="PLL59_09345"
     CDS             complement(5321..7402)
                     /locus_tag="PLL59_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMY24055.1"
                     /translation="MKRILLFLLFTNVFSTLFSIEVNTKNIKIIRDKFGVPHIYGKTD
                     EEVAYGLAWATAEDDFKSMQENFLTARGRLAEVKGKDGAIMDFVCAVLGARESVEKLY
                     DKSYSPKFKNMLSAYVKACNRYAALHPEKVWIKNAFPVTEKDVVVGYAIGLALMTNVP
                     FSIMKINDGTIENKTLSSPKGSNAFAVNSKKTKDGNTYLGINSHQPLEGPYSWYEAHL
                     HSEEGWNILGGTFPGGVTIFHGANEHLGWAHTVSFADHDDVYALKMHPTDKRKYEFDG
                     KWLVLQERKVKLKVKLFWFIKIPITKKYYESVYGPVLEKEGKFYALRFPSAFDIRGAE
                     QWYHMNKATNLNEFQGALKQMALPGMNIIYADKNDNIMYIDNGHFPRRAKGYDWWHVV
                     PGNTYKTLWKPNDYYGYDSLAKVINPACGYLFNNNNTPFNCTAKEDNLKKETQPLNEY
                     YFAYNDNRALRTSYLFASKGKISYEEFKQIKYDQSFMNPAFNYAMINIEDVLHLDTQK
                     YPHIKEGIDVLKKWNRSADVNNKQAAMVALITKYVIDRLVAEGNFPAVRTRIKEWFLV
                     QCVADAQEHLLKHFGQLEVPLGDMQKLVRGNKELPIGGVPDVNATMWISKHKNGKYKA
                     EAGETYIELIQFTKNGPIIESISPYGTSNVEGNKHYDDQMELFVQQKCKKMSMNLAEI
                     IKEKESEYHPQ"
     gene            complement(7537..8586)
                     /locus_tag="PLL59_09350"
     CDS             complement(7537..8586)
                     /locus_tag="PLL59_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HMY24056.1"
                     /translation="MKTNVKLLAQICEVAGAPGHEQRVREIVLKNIKGVADKIEQDNL
                     GNIYAIKKGKDSSKRVMIAAHMDEIGFMVKHIDNNGFLRFHTLGGFDPKTLTAQRVIV
                     HGKKDLIGVMGSKPIHIMTPEERNKPVHIHDFFIDLGLPYKEVIKYVSVGDVVTRDRQ
                     LIEMGDCVNCKSIDNRVAVYILIETLKLLKDCPYDVYGVFSVQEEVGIRGANVAAHNI
                     NPTFGFGLDTTIAYDLPDSKPEEQITKLGEGVAIKLMDASTICDYRMVKFQKEIADKF
                     KIKWQVELLPMGGTDTAGIQRMGKNGAIAGAISIPTRHLHQVIEMAHKSDIEASIQLL
                     KHCIENLHKYDWSLK"
     gene            8739..>10405
                     /locus_tag="PLL59_09355"
     CDS             8739..>10405
                     /locus_tag="PLL59_09355"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMY24057.1"
                     /translation="MSVLNNISGAVLKPVLKEISRTRLPKIDGKLSISGLKEDVEIIR
                     DELGIAHIYANNINDLMFAQGYVHAQDRLFQMELNRKVARGTLSEIIGIKALDSDRIA
                     RTMGYERVAKLDWELFDESEQQIIIDYCHGINAYITSADFKLPIEFTLLKFKPEKWHP
                     MDVASFSRLLISLLTWGWYDEIIRAKLIEAVGAAAAAELDNTYPKENPITLPKGIEFN
                     ILDVSGKFQAMKGPYMPQISGSNAWTLSGTKTKTGKPFLCNDPHLTLKNPNIWYLNHL
                     HCPDLHASGVTAPGLPMVQIGHNENIGWGITLSYTDIEDVFVEKFTDDTCTTYIHEGK
                     LNDTTIIEEKIFVKGEKMPIIEKVYQTVHGTLISDVTGHSNMKLTLCSMAYQAGTSTK
                     GWFLLNKAKNWNEFADAVKYITAPGLNIVYADKDNNIGYYNSGKVPVRDKASASVPQP
                     GWTGEHDWKAFIPFDEMPHSLNPQNGYIVTANHKIEPDDFPHFLGDIYMNGYRANRLE
                     KMIQRKEKLEPKDFTEMQMDFYCTPGKLFAAHFKNLQMENAELQQYV"
BASE COUNT         3341 a         1843 c         1853 g         3368 t
ORIGIN      
        1 gctgctgatg atgcacaggc tttggcagca gaattaaatg gcgattcgta tttattagat
       61 atttcacaag ctactgctgc aaaagaatta caaaaataca ttatcgagaa atatggaaaa
      121 ctagatattt tagtaaataa tgcaggcatt actcgcgata aaacgattgc aaaaatgagc
      181 atcgaccaat ggaggagtgt attaaatgtc aatctaaaag ccgtaattca acttacacaa
      241 atatttatcg aaaatggatt cagtaaagat gctaaagtcg tgagtctttc ttctatttct
      301 ggtatttctg gcaatgtagg ccaaacgaat tactctttaa caaaagctgg cgtaataggt
      361 ttttctcaag ctttagcagc aacaaataaa aatatttttg ccaatgccgt tgcacctgga
      421 tttattgaaa caaagatgac agaaaactta cctttcttta ttaaagaagg tggtcgccga
      481 ttgtcaacgc ttaaacaagg tggtaagcca gaagatgttg ctgagttgat tggtttcttg
      541 gcatcgccat tgtcaaatgg tatcaacgga cagtgcgtac gtgtttgtgg aggtaatatg
      601 attggtgcat aatcttaaat agtaagataa aaagtccatt ttgttcatat ttttcttact
      661 tttttgtaat attatacctt tttaacatta tttttaagca attagaggat tcgttctcta
      721 ttatcataac atcgaataaa aaaacaaact catgaaaaga ctattcatta caggattatt
      781 agcggtatta acgctcagca tttttgctca atccacaaca acatccaacg aaactgtaaa
      841 attaaaaatc agaaaaaaag ttgaccgacc aactgataaa tactggtaca tgacgttgag
      901 tggcggttac ggtattccgt ttttagccac aaataagcgt tcacctttaa aagaagttgg
      961 cgacaaacaa tggtatcaac atggaaatga cttgagtgta aaatctaaat tcggcacaaa
     1021 tggtggtggc tttgctttta acgttggctg gggacacatg tttaataaat acataggcat
     1081 cgacgtgttg cacactttcg cttggcaccc agaatcttta gctgctaaaa tagatttaga
     1141 tacttactat gccacacaaa aaacaggtac tttcagtatt tatgtttctc cacacttagt
     1201 aatgcgttgg gacaatggca aacgtttcgg catcacagga aaagctggct tagtattgcc
     1261 aatatttggt aaaactactt caagagccta catcttagat aaacaaggtc gtgtaatcga
     1321 aaccttggca ggtttgccaa tcttacctct aagtattcca ggattattag gcttagaaat
     1381 tgaaatcgtt ggtaaagcgc acacagagta tcatccaact gttggcgtga gtgcgtctat
     1441 cggcttcgat gtgaaattaa ataaacaatt gtcattgttt gcagaaatgc gtgtgcaagc
     1501 ttatacaatc tcattaagag aaaccatctt tgatgagttt aagcaaactt caaaaattaa
     1561 tgtattaggc attaaattag atgcaccaga aggtatgctg gcaattccat caaatgtagc
     1621 aaatgtaagc gaagcaccag aattcttaaa acactacgtg tatagaaaag aactgaacga
     1681 aaattctaac acagctcgat atggtacgag aagtttattt gcagaagatg gattaggcgg
     1741 cttagtacgt acgctaactg gccaaacagg tcttcctagt gttgatatca acaagccaat
     1801 ggaagagcct ggtcaaaaat tcaatgcatc aagtttgtat tttaacgcag gtcttcgtgt
     1861 aaactttgac cattggaata aaaaacgtag ttcaaaacgt gttgctcaag catcaaaatc
     1921 atcttccaaa ccttcaaaat caaaaaagtc aaaaagttca aagtcaatgg tggacgatcc
     1981 agatagtaca attaatgttc aataatcaac taaaattatg aaaagaaaca ttctgattat
     2041 ttcagcgata ctgctatttg cagcgccagt ttttgcacaa aagaaaaagg caaagacgat
     2101 gtctttatct tctacagttg gagacaaaaa gggagatgtt agcgaaagca agttgaaaat
     2161 aagaaagaat gtaacaagac cagagggaaa gggttggttt atgtatggtt ctgctggtta
     2221 tggcattcca tttttaagca ccaataaatt ttcgccattc aaagaaattg gaaataaaga
     2281 ttggtatcaa acacccaact cattaaacgt taaatctatt tttggtactc taggtggagg
     2341 ctggcaggcg agcattggct ggggacacat gttcaacaaa tatattggta ttgatatatt
     2401 gcatacgcta tcttttcatg cagaacaatt agatgcacgt attaacactg atattgattt
     2461 aggtggtgga aaaagagcat cgtattttgc agaagaatac actaaaatta tgccggctat
     2521 ttacttaaat cctcatttag taatgcattg ggataatggg aaaagatttg gtgttaccgg
     2581 caaagcaggc ttgtgtatgc ctatgggtgg cacaccagtt acacgcgcta agatcataga
     2641 taaatcaggc agattagtgc aaacacttgc aggattgcct gtgattcctt tgacagcttt
     2701 agccgacttt agctcagaat atacagcgac agcaaaatca aaattaaaac caaccattgg
     2761 cttgagtgct tcaattgcct ttgatgtacg cttgtttaaa aatgtatggg catttgcaga
     2821 gattcgcgta caagcattta caatttcacc aaaagaaaca gaatttacag agttttcttt
     2881 aaaaacagaa tcaccattac tgccaattat ttccgcacta acaccgttgc cactgaatgt
     2941 tgccaacatc aatgaaacgc ctgtttatct caaacacttt gtgtatgtaa aagaaattac
     3001 agaagaatca aatacgatgc gttacagcag tggtctctca ttaaaagaaa ttgatatgaa
     3061 taaaccaatg gaagaacctg ccgtaaaata caatgcctcg actttgtatt ttaatgcagg
     3121 cgtccgtatg aattttacac aaaattggga aaaacgcaga gcagctaaag taattcctaa
     3181 acgcgaagca aaagctgcta aaaaaaataa ataatgaaaa ttatagctaa caggaagact
     3241 catacacaag gcgtgcatga gtcttttttt ataaatagat tagtatgaaa ataaagtgga
     3301 ctatacttgg atgcttactc ctaattcaat ctagtattgt attcgcaggc tttacaacag
     3361 gaacttttaa atacaaaggt gcatggcgaa aatatgccat ttatgtgcca gcaatttatg
     3421 catcgagttc agaaaaagtg ccactgttat taggtttaca tggctatggc gatgatatcg
     3481 acaatttcag aaatatttgt atgaccaaca ttgctgatac cgcaaattat atttgtgtgt
     3541 atgcagaagc attgccgtgg ttaggcgcaa atgcatggaa ttcaggtgct ggtgttggta
     3601 tcattaatgt taattcagaa gtagatgatg taggcttttt aaatagttta attgatactg
     3661 ttatctcaaa atatagaata gatacaacac gtatgtatgc gttcggattt tcttttggcg
     3721 gttttatggt cgataggtta gctacagaat gcagtcagcg ttttgcagct gcatgtaatg
     3781 tatctggttt gcatggcaat tttttagcag gaaaaattcc aagcacatac gtgccttatt
     3841 taaaatttca tggaacaaac gatccaacta tttcctataa cggcatgcag acagtcggtc
     3901 tgtttccagg ctttggctta agtgcagaaa atacagtgaa attttggatg acacaaaatc
     3961 attgtgatac aattcctatt gtcgattcta tgccgaatac agcaaatgat ggattgcgat
     4021 ttattcgttt tacttataat aacgactata ataacagtaa agtaattttt tataaggtgc
     4081 taaatggctt acataaatgg tatggattgc cagcaaatga tatttcttat tgtcaaacaa
     4141 tttgggattt ctttagacaa tataagagag aaaattttgt cacggcaatt agaaatatta
     4201 atcatgtcga acaaataaaa atttttccaa atccatcaaa tggtaacttt acagtatcat
     4261 tacaaaatct gaattataac attcaacaaa tttctatttt tgatatagct ggtaaagaag
     4321 tatatacaca agcagtgaca acacaatcac ccataacgat agtaagtaat ttgccaaaag
     4381 gcttgtattt attgcgctta gtcgataatg aaaaacatgc aatgacggaa aaaattatta
     4441 ttgaatagaa tacagttatt aaacaaaacg caagtggaat aattgtgtca tatcaaaaaa
     4501 tctattaaac tgcatagcaa cttttgtgct tagtcgctgg ttagattttg gcattgcttc
     4561 aaaaaatttg atggttcgta ctagttcaat atgtttactc caatcggtta ccgttttagc
     4621 atctaaaatt ccccattcta aatgtgcctc tttcatgcca gctgctttga tacgcatatc
     4681 tacaaagaat tttccgagct gagaaattac atcaaacata atttcagcac cttttaagcg
     4741 tggtgcaatg gtggataaaa atactcttac atcttcttct ttaaaataag gcaatacacc
     4801 tggaattgta ataaacaaac cattactgat atcgccaata tcatctatcc aagagtaatc
     4861 taacatagat tttgcgatgt agtttacgcg cttatttggt ggtggcaaaa gttgtgtgcg
     4921 caatgcaatc gaatcaggta aatctaaatc ataccaaagg gctttgccat tatcacatcg
     4981 ataataggtt gtgtctaagc cagcaccgat gtctaatatt tttccattag gatgtttttg
     5041 taaaaacgct tttatcgctt catccatttt ataagcacga taacataaac catgaacacc
     5101 aatttctgtt atgtttcgca ataatttttt cttgtcaaca tctatttgtt taaataatcc
     5161 aacagctttc gtgtcattca ataatggatt tttatattca gtttcctttg cgcgtgaaac
     5221 caatggcaac agtaatgttt cttgtactct tcctaatttt acttttattt ttgtgctcat
     5281 aatttgatgt caaaatcttg tttgtgaagt aaagatataa ttattgtgga tggtattcac
     5341 tttctttttc tttaataatt tcagctaagt tcatgctcat ctttttgcat ttctgttgta
     5401 caaataattc catttgatcg tcgtaatgtt tgttgccttc aacattgctt gttccatacg
     5461 gcgatatgct ttcgatgatt ggaccattct ttgtaaattg gattaattcg atatatgttt
     5521 cgccagcttc tgctttatat tttccgtttt tatgcttgct aatccacatg gttgcattta
     5581 catctggcac gccaccaatg ggcaattctt tgttgccacg aaccagcttt tgcatgtcgc
     5641 ctaatggtac ttcgagctgg ccaaaatgtt tcaataaatg ctcttgcgca tcagcgacac
     5701 actgtactaa aaaccattct tttattcgcg tgcgcacagc aggaaagttc ccttccgcaa
     5761 ccaatctatc aattacatat ttagtaatta atgctaccat tgccgcttgt ttattattga
     5821 catcagcgct tctattccat tttttcagca cgtcaatacc ttctttaatg tgcggatatt
     5881 tttgtgtgtc taaatgcaac acatcttcaa tgtttatcat agcataatta aatgctggat
     5941 tcataaatga ttggtcgtat tttatttgtt taaactcctc atacgaaatc tttccttttg
     6001 aggcaaacag ataacttgtc cgcaatgctc gattgtcgtt ataggcaaaa taatattcat
     6061 tgagtggctg cgtttctttc tttaaattat cttcttttgc cgtgcagtta aaaggtgtgt
     6121 tattgttgtt aaatagataa ccacaggcag gattaattac ttttgccaat gagtcgtaac
     6181 catagtaatc attgggcttc caaagtgttt tatacgtatt tccgggcacc acatgccacc
     6241 aatcataacc ttttgcacgc ctaggaaaat ggccattgtc gatatacatt atgttgtcat
     6301 ttttgtcagc atatatgata ttcatgccag gcaatgccat ttgttttagt gcaccttgaa
     6361 actcgtttaa gtttgtagct ttattcatgt gataccattg ctccgcacca cgtatgtcaa
     6421 atgctgacgg aaatcgcaag gcataaaatt tgccttcttt ttctaaaaca ggaccataaa
     6481 cactttcata atattttttt gtaataggga tttttataaa ccaaaatagt tttactttta
     6541 attttacttt tctttcttgt agcaccaacc attttccatc aaattcatat ttccttttat
     6601 ctgtcggatg catttttagt gcatatacat catcgtggtc ggcaaagctt acagtatgcg
     6661 cccaacctaa atgctcattg gcaccatgga aaatagtaac gccaccagga aaagtgccac
     6721 ctaaaatatt ccaaccttct tcagaatgta aatgtgcttc gtaccaagag tatggacctt
     6781 ctaaaggctg atgcgaattg atgccaaggt acgtattgcc atcttttgtt ttcttgctat
     6841 ttacagcaaa tgcattgctg cctttaggtg agctcagcgt tttgttttca atagtgccat
     6901 cattgatttt catgatagaa aatggaacat tggtcatcaa agctaaacca attgcatagc
     6961 caacaacaac atccttttct gtaactggaa atgcgttttt tatccatacc ttttctgggt
     7021 gtaatgcggc atatcgatta caagctttaa cgtaagcact caacatgttt ttgaatttag
     7081 gagagtagct tttgtcatat aatttttcca cgctttcgcg agcgcctaac acagcacaaa
     7141 caaaatccat aatggcgccg tctttacctt tcacttctgc gagccggcca cgagcagtta
     7201 agaaattttc ttgcatactt ttaaaatcat cttcagccgt tgcccatgct aagccatacg
     7261 ctacttcttc atctgtcttg ccataaatgt gaggcacgcc aaatttatct cgaattattt
     7321 tgatgttttt ggtgttgacc tcaatagaaa atagcgtcga aaatacgttt gtaaaaagta
     7381 agaatagaag aatccttttc atcatctttc tgttgatttg taatagaaag atacaaattt
     7441 atctgtgtgt ttttttattt tttcactagt tatgctacac aaaaaaaaga gtcttgattt
     7501 ctcaaaactc ttttctattt taactaaaca gaattattat ttcaaactcc aatcgtattt
     7561 atgtaagttt tctatacaat gttttagtaa ttgtatgctt gcttcaatat ctgatttatg
     7621 cgccatttct atcacttggt gtaaatgacg agtcggaata gatatggcac cagcaatagc
     7681 accatttttt cccatacgct gaatacctgc tgtatcggtg ccacccattg gtaacaattc
     7741 cacttgccat tttattttaa atttgtcagc aatttctttt tggaatttta ccattctata
     7801 atcacagata gttgacgcat ccatcaattt aatggcaaca ccttctccta attttgtgat
     7861 ttgttcttct ggtttagaat caggtaaatc gtaggcaatt gtagtatcta aaccaaaacc
     7921 aaacgtagga ttaatattgt gagcagcaac gttggcacca cgaatgccta cttcctcttg
     7981 tacggagaaa acaccatata catcataagg gcaatctttt agtaatttca atgtttctat
     8041 taatatataa acagcaacac gattgtcgat agatttacaa ttgacacagt cacccatttc
     8101 tatcagctgt cggtcgcgtg taactacatc acctactgaa acatatttta taacctcttt
     8161 ataaggcaaa cctaagtcta taaaaaaatc atggatatga actggtttgt tgcgctcttc
     8221 tggtgtcata atgtgaattg gtttactgcc cattacacca attaaatctt ttttaccatg
     8281 tacaataaca cgttgtgctg ttaatgtttt tggatcaaaa ccacctaagg tgtgaaagcg
     8341 taagaaacca ttgttgtcaa tatgttttac cataaaacca atctcatcca tgtgtgctgc
     8401 aatcatcaca cgtttactac tatcttttcc tttttttata gcataaatat ttccaagatt
     8461 atcttgttca attttgtctg ctacgccttt gatatttttt agtacaattt ctcgtactcg
     8521 ctgctcatgt cctggagcgc ctgctacttc acagatttgc gctaatagtt ttacatttgt
     8581 cttcatacct ttttatataa tgaggtttta tgtccttttg tttaaaatag ggtttcaaat
     8641 ataattattt attccatgat tacttacttt tagtctattt ttttgaccta aacctagtaa
     8701 tttgtttatt ttctatcttt aagcactaaa ttttaataat gtctgtatta aataatataa
     8761 gtggtgctgt actaaaacct gtcctaaaag aaataagtag aacgcgtttg ccaaagattg
     8821 atggcaagct cagcataagt ggattaaaag aagatgtaga gataataaga gatgagttag
     8881 gtattgcaca tatttacgct aataatatta atgatttgat gtttgctcaa ggttatgtac
     8941 atgcgcaaga tcgtttattt caaatggaat taaatcggaa agtagcaaga ggtacactga
     9001 gtgaaataat aggcataaaa gcactagaca gtgaccgtat tgcccgcaca atgggctatg
     9061 aacgtgtggc aaaattagat tgggaattat ttgacgaatc ggagcaacaa attattattg
     9121 attattgcca tggtattaat gcttatatca ccagtgccga ttttaaattg ccaatagagt
     9181 ttactttgtt gaaatttaaa ccagaaaaat ggcatcctat ggacgttgcc tcttttagta
     9241 gattattgat ttcattgctg acttggggct ggtatgatga aattatcaga gcgaaattaa
     9301 tagaagcagt tggcgccgca gccgctgctg aattagacaa tacgtatcct aaagaaaatc
     9361 caattacatt accaaaagga atagagttta acatcttaga tgtttcagga aaatttcaag
     9421 cgatgaaagg tccatacatg ccacaaatta gtggtagcaa tgcttggaca ctcagcggta
     9481 ctaaaacaaa aacaggaaag ccttttttat gcaacgatcc acatctcact ttaaaaaatc
     9541 cgaatatttg gtacctgaat catttgcatt gtccagactt gcatgcaagt ggcgtaactg
     9601 ctccaggttt gccaatggtt caaattggcc ataatgaaaa tataggctgg ggcattacgc
     9661 tatcttatac agatatcgaa gatgtttttg tagaaaaatt tactgacgat acttgtacaa
     9721 cctatattca tgaaggaaaa ctcaacgata ctacaattat agaagaaaaa atatttgtaa
     9781 aaggagagaa gatgcctatt attgaaaagg tatatcagac cgtgcatggc actttgattt
     9841 ctgatgtaac aggtcattct aatatgaaac ttactttgtg ttctatggcc taccaagctg
     9901 gcacttctac taaaggttgg tttctgttga ataaagcaaa gaactggaat gaatttgctg
     9961 atgcagtaaa gtacatcacg gctccaggtc taaacattgt gtacgccgat aaagacaaca
    10021 acataggata ttacaattct ggcaaagtgc cggttcgcga taaggccagc gcatctgtgc
    10081 cacagcccgg ttggactggt gagcatgatt ggaaagcatt tattccgttt gacgaaatgc
    10141 cacattcact aaatccacaa aatggatata ttgttacagc aaatcataaa attgagccag
    10201 acgattttcc gcatttctta ggagatattt acatgaatgg atatcgtgcc aatcgattgg
    10261 agaaaatgat acagcgaaaa gagaaattag agccaaaaga ttttactgaa atgcaaatgg
    10321 atttctattg cacacctgga aaactgttcg ctgcgcactt taaaaatttg caaatggaaa
    10381 atgcagagtt gcaacaatac gtaga
//
LOCUS       DAOQQV010000091        10213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2401_length_10213_cov_6.774660, whole genome shotgun sequence.
ACCESSION   DAOQQV010000091 DAOQQV010000000
VERSION     DAOQQV010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_10213_cov_6.774660"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1078)
                     /locus_tag="PLL59_09360"
     CDS             complement(158..1078)
                     /locus_tag="PLL59_09360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY24058.1"
                     /translation="MEISVVIPVFNEEANLPLLHERLVNAVTSCSNDYEFIFVNDGST
                     DKSLQIIRDLAARFAQVKYIDFSKNFGHQLAVFAGLEHATGEYIVIIDADLQDPPELI
                     QELYAKIKTGFDVVYAQRETRVGESWHKRFTAKLFYRFINTLSDVAIPLDTGDYRILT
                     KRIKDIVVAMPERNKFLRGQIAWTGFNQSSVMYKRDERYSGKSNYSYTKMFSFAIDGI
                     TAFSNLPLRLATYLGFLVSLISFFVILYTLYQKYINHNVVQGWSSLMVSVMFIGGVQL
                     ICLGIIGEYLSRILDNVKQRPLYIVRDSNL"
     gene            complement(1102..2475)
                     /locus_tag="PLL59_09365"
     CDS             complement(1102..2475)
                     /locus_tag="PLL59_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24059.1"
                     /translation="MKSSNRNILLFAIVVAVLVNMLSWNFPFFWDTILTSTITHSIFE
                     HGGLLPPPSIDAGHPPLFYWYIVLMYQLFGYSLQVAHLAMLPFTIIGALTFILLLQRF
                     QFSKQQQYFAVLFYFSIPAVFTQHTMLSYDVVLLSMYCAALTAFLYQRKIFLSLISIV
                     LVGVSLRGVVCLFAISMTIYFFEKRKFKLWWHWNLYFIPALILSFIWYYFHYTQTGWW
                     ISTPSIAWSQHRSFVDAKGFAVNLFSIARCFFDVGIFILSVLTALYFIKVKKLDKLMM
                     IWCIPALIFILFFIPFSNPINHRYFLIVYVFLLLPVLKFLRDKKVYVQFIFLFIVILG
                     HFQIYPVPISNAWDCTMQYTNYSSVKQEAENFITSKNINRDAIATVFPMNASFLQTNN
                     KVGYARLENIHAHAIDSIPYILFSNVGNDFSDTQIKQLQQWHQIASFDKGLIHMILYK
                     NPVFSNY"
     gene            2549..3385
                     /locus_tag="PLL59_09370"
     CDS             2549..3385
                     /locus_tag="PLL59_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HMY24060.1"
                     /translation="MQTLTLAISPCPNDTFIFDAMLHHKIDTEGFDFKLIFADVEQLN
                     QAAFAQQYDITKLSYHAFAYAIENYVLLRSGSALGNNCGPILIAKNHLTDEQMQSCSI
                     AIPGKYTTANLLFSLRYPTANNKHFVLFSDIEKKLLQEEYNAGVIIHENRFTYEGHGL
                     KKIIDLGEFWESTYHAPIPLGGIAVKRSLEIEVQLAIERIIKRSIEFAFANPTSSKTF
                     VSQHAQEMNEEVCKKHIDLYVNKYSVALGFEGELAVNTLYKIAHENKIIPTFYDSIFI
                     EK"
     gene            complement(3391..3945)
                     /locus_tag="PLL59_09375"
     CDS             complement(3391..3945)
                     /locus_tag="PLL59_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HMY24061.1"
                     /translation="MQRLYIFLFSILLFINLFAHSSNKKDKYDEFMYWNADSKLSWSD
                     FKGKPATGTSEEAMTASSVQYNYSISGGKIQWQVYAKFYPNASWKSLKVYSDYLLQHE
                     QLHFDISELYARMLRKQLTEKIKSSKDASLLKKIGDSIINAWDIEQDAYDRETNHSMN
                     EKSQAIWQAKIHERLAELQEFASP"
     gene            complement(3991..5583)
                     /gene="bshC"
                     /locus_tag="PLL59_09380"
     CDS             complement(3991..5583)
                     /gene="bshC"
                     /locus_tag="PLL59_09380"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HMY24062.1"
                     /translation="MQEINIPFSETGMFSKLILDYVNSVETTHLFYNYSLDINAIQQA
                     ITDKQNEKIDRDNLVHTIQRQYANIELSDAVASNINCLSNSNSYCIVTAHQLNIFGGP
                     LYYIYKIAQTISTCQQLKLKYPDISFVPIYWLGSEDHDFEEINHIYLYNKKIEWQDKQ
                     GGATGNYNPESILPLIDEIKSILHVDEDNDLIQLFRSAYAQNSLTNATRYLLHSLFGK
                     YGLVIVDGNDASFKQAFSDVMQDELLQQQSITLVEQQLQLLASKGYKQQAFPRAINLF
                     YLFKNARERIEFNASTSTYSVVNTNITFLQNEIIDELKNNPERFSPNVILRPLFQQKI
                     LPALAYIGGAGEISYWLQLKTLFQHYKVNFPQLIVRNSVLLINDALTKKAMKLGFTTQ
                     DFFQDIDLLKKIYIEKNTATDLDFSNYKHDIEKVFNEIKNKTQSIDVSLVNVVGAEQQ
                     KTLQSIDAIQKRLLKSLKQKNENELLQIEKLKQQLFPNQSLQERVENFSSYYVKYGQS
                     FIDELIQRFDIYNKQFLLLTTI"
     gene            complement(5622..6932)
                     /gene="rimO"
                     /locus_tag="PLL59_09385"
     CDS             complement(5622..6932)
                     /gene="rimO"
                     /locus_tag="PLL59_09385"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HMY24063.1"
                     /translation="MKTKSHTKDKYNVITLGCSKNAVDSEVLVAQLKHNEFDVEHQSA
                     SKDANIIIVNTCGFIDRAKEESINTILSYAEQKDKGRIDKLYVTGCLSQRYKDDLEQE
                     IPQVDAYFGTLELPQLLQKLNADYKHELVGERVLTTPQHYAYLKISEGCNRTCTFCAI
                     PIMRGKHVSKTKEDIVAEAKNLAKQGVKEIMLIAQELTYYGLDIYKERALSKLLYALN
                     ELEGIEWIRLHYAYPSKFPLDILDTIKDCDKVCKYLDLPLQHANNNVLKRMQRQITQE
                     ETKALIQHIRTKIPNIAIRTTMLVGFPGETEDDFNDLCAFVEEMKFERLGVFQYSHED
                     DTAAFELDDDIDADTKEERAAKLMQVQQEISFLKNEEKIGNTYKVLIDRKEGGYFIGR
                     TEYDSPEVDNEVLLDANKNYCRIGDFCIAKITDATDFDLYAELV"
     gene            complement(6977..7924)
                     /locus_tag="PLL59_09390"
     CDS             complement(6977..7924)
                     /locus_tag="PLL59_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24064.1"
                     /translation="MRKIFCIFILLVQFILSFSQKSVSYYRTIEYFDKTDSAKNYAVF
                     YRPNGPAKKMLILLTGFGESPQIAEIETNIPEIAASKGILTAIICNTDGSQSFSIDGN
                     AQHYLDSIIPLLLTRNNIPNDAYYIGGFSLGGTSAIKYVQHCNIYDIQPMPKAVFTID
                     PPLDFVRMHKIYEHWKNDTSKWYNTNKPLYTIYLDKMHTFFKGNLKDAFENYVNLSPY
                     CYEDSNNYGARLFGKMPLLTYCEPDIDWAMNEKRWNLYELNLADNVGFINELKQLGNT
                     NSPLILTKGKGYRKVLKYKQPHSWSIADANEVVKWLLKF"
     gene            complement(8001..8978)
                     /gene="ftsY"
                     /locus_tag="PLL59_09395"
     CDS             complement(8001..8978)
                     /gene="ftsY"
                     /locus_tag="PLL59_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HMY24065.1"
                     /translation="MGIFDKIFGKTKSEEEIKIEEKQLNEGLEKTKQGFFSKISKAVA
                     GKSTVDIEFLDELEEILISSDVGLSTTIKIIDRLEARVSRDKYLNTNELNKILKDEIG
                     NILAENNTVDLLNFAPNTDKKPHVIMVVGVNGVGKTTTIGKLAYQLKKEGKEVLLGAA
                     DTFRAAAVDQLTIWSERVGVPIVKQAMGSDPAAVAFDTVQSGIAKNADVVIIDTAGRL
                     HNKQGLMDELSKIKKVMSKLIPDAPHEVLLVLDASTGQNALEQAKHFSAATQVTALAL
                     TKLDGTAKGGVVLSIADEFKIPIKYIGLGEKMEQLQVFNRKVFIETLFE"
     gene            complement(9073..9243)
                     /locus_tag="PLL59_09400"
     CDS             complement(9073..9243)
                     /locus_tag="PLL59_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HMY24066.1"
                     /translation="MAKVSKNAKVDRGKNVGESKNMVKIIKFVRSAKTGAYSTREKVV
                     HKDLLQDTVKSL"
     gene            complement(9250..9435)
                     /gene="rpmG"
                     /locus_tag="PLL59_09405"
     CDS             complement(9250..9435)
                     /gene="rpmG"
                     /locus_tag="PLL59_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HMY24067.1"
                     /translation="MAKKKGNRIQVILECTEHKTSGLAGISRYITTKNRKNTPERIEL
                     KKFNPIMKKVTVHKEIK"
     gene            complement(9448..9693)
                     /gene="rpmB"
                     /locus_tag="PLL59_09410"
     CDS             complement(9448..9693)
                     /gene="rpmB"
                     /locus_tag="PLL59_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HMY24068.1"
                     /translation="MARFCDLSGKGPMTGNSVSHSNIKTKRRFYPNLQKKAFFIPELD
                     RWIELKVSTSAIRTINKKGIYSYLKELEKKGEIQLGF"
     gene            complement(9790..9863)
                     /locus_tag="PLL59_09415"
     tRNA            complement(9790..9863)
                     /locus_tag="PLL59_09415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9827..9829),aa:Arg,seq:acg)
BASE COUNT         3372 a         1727 c         1570 g         3544 t
ORIGIN      
        1 gaaagaagaa ataaagataa ttggcagata aaaaatgctg tcgacaattt gaataaggaa
       61 ataatagcac tgttgagtta gttgacaatt tcaatagttt tttaatttat tgaattcagc
      121 aatacacttg ttgcattcct tttatttatt atacgaatta taaattactg tctctaacga
      181 tatagagtgg acgctgtttg acattatcta aaatcctact taaatattcg ccgattattc
      241 ctaaacaaat taattgcaca ccaccaataa acattacact caccatcaat gaagaccaac
      301 cttgtacgac attatggttg atatattttt gatatagtgt atatagaatt acaaaaaaag
      361 aaattaatga caccaaaaaa ccaagataag ttgctaagcg caatggcaaa ttggaaaatg
      421 cggtaatacc atcaattgca aatgaaaaca tttttgtgta tgaataattt gattttccag
      481 aatatcgttc atcgcgctta tacattacag aagattgatt aaatccagtc catgcaatct
      541 gaccacgcaa aaatttatta cgctctggca tcgccaccac tatgtctttt atgcgtttag
      601 taagtattcg gtaatcgcca gtatctaaag gtatagccac gtctgacaat gtattgataa
      661 agcgataaaa taatttagca gtaaatcgct tatgccaaga ttcgccaact cttgtttccc
      721 gttgtgcata tacaacatca aaaccagttt ttattttagc atacaattct tgtatcagct
      781 caggtggatc ttgcaagtca gcatctatga tgacaatgta ttcgccagta gcatgttcta
      841 agcctgcaaa aacggccaat tgatgaccaa aatttttact aaaatcaata tattttactt
      901 gtgcaaaacg tgccgctaaa tcgcgaatta tttgtaatga tttatctgtt gaaccatcat
      961 tcacaaaaat aaattcatag tcattggagc acgatgtaac agcatttact agtcgttcgt
     1021 gcaacaaagg aaggtttgct tcctcattaa aaactggaat gacaacagaa atttccataa
     1081 accaaatgta ctattttcaa ttcaataatt agaaaaaaca ggatttttat acaatatcat
     1141 atgaattaat cctttatcaa aggaggctat ttgatgccat tgttgtagct gtttaatttg
     1201 cgtatcggaa aaatcatttc caacatttga aaacaaaata tacggaattg aatcgatagc
     1261 atgcgcatga atattctcta gtcgagcata gcctacttta ttattggttt gtaaaaagct
     1321 tgcattcatt ggaaaaacag ttgcaattgc atctctgttt atattttttg aagtaataaa
     1381 attttcggcc tcctgcttta cactactgta attggtgtat tgcatggtgc agtcccaagc
     1441 atttgatatc ggaacaggat aaatttgaaa atgcccaagt atgactataa acagaaatat
     1501 aaattgtaca tacacttttt tgtctctcag gaattttaaa actggcaaca ataggaatac
     1561 atacacaatc aaaaaataac gatgattgat tggattggaa aatggaataa aaaacaaaat
     1621 aaatataagt gctggaatac accatatcat catcaattta tctaactttt ttacttttat
     1681 aaaataaaga gcagtaagta ctgaaagtat aaatattcca acatcaaaaa aacaacgtgc
     1741 tattgaaaat agattaacag cgaaaccttt tgcatcaaca aaactgcgat gttgactcca
     1801 agctatcgat ggcgtgctta tccaccaacc agtttgtgtg taatgaaaat aataccaaat
     1861 aaaagataat attaacgctg gaataaaata caaattccaa tgccaccaca acttaaactt
     1921 tcttttttca aagaaataaa ttgtcataga tatcgcaaac aaacatacta caccacgtag
     1981 tgaaacgcca actaacacta tcgaaattag actaagaaaa atttttcttt ggtataaaaa
     2041 tgcagtaagt gcagcacaat acatagacag caaaacgacg tcgtagctca acattgtatg
     2101 ttgtgtaaaa acagctggaa tagaaaaata gaataataca gcaaaatatt gttgttgttt
     2161 gctaaattgg aatctttgta aaagtagaat aaaagtaagt gctccaataa tagtgaatgg
     2221 caacatagct aaatgagcca cttgcaaact atagccaaat aattgataca tcagcacgat
     2281 ataccaataa aataaaggcg gatggccagc atctatgctt ggtggtggca ataaaccacc
     2341 atgttcaaat atcgaatgtg taatggtaga agtaagaata gtatcccaaa aaaatgggaa
     2401 attccacgat aacatattga cgagcactgc aactacaata gcaaacaaca gtatatttct
     2461 attggaagat ttcattctaa tgcaaagtac ataaagatgt acaatatgca tcattttaaa
     2521 agttaaatat tagccttaca tttatgatat gcaaacttta actttagcga tttcaccttg
     2581 cccaaatgat acattcatct tcgatgccat gctgcatcac aaaatagata ctgaaggttt
     2641 cgattttaag ctgatatttg ctgatgttga acaattaaat caagcagcat ttgcacaaca
     2701 atatgacatc acaaaactta gctatcatgc atttgcttat gccattgaaa actatgtatt
     2761 attgcgatca ggcagtgcgt taggaaataa ttgtggacct attttaatcg ccaaaaatca
     2821 tcttactgac gaacaaatgc agtcttgttc aattgcaatt cctggaaaat atacaacggc
     2881 aaatttattg ttctccttac gttatccaac tgcaaataac aaacattttg ttttgttttc
     2941 tgatattgaa aaaaaactct tacaagaaga atataatgct ggtgtgatta tacacgaaaa
     3001 tagatttact tatgaaggac atggcttaaa aaaaattatt gatttaggtg aattttggga
     3061 aagcacgtac catgcaccaa ttccgcttgg tggcatcgct gtaaaaagaa gtttagaaat
     3121 agaagtgcaa ttggctattg aaagaatcat aaaacgaagt attgaatttg catttgccaa
     3181 tccgacatcg tctaaaacat tcgtatctca acatgcacaa gaaatgaatg aagaggtttg
     3241 taaaaaacat atcgatttat atgtaaacaa atacagtgta gcactaggat ttgaaggcga
     3301 acttgccgtc aatacacttt ataaaattgc acacgaaaat aaaattattc ctacttttta
     3361 cgattctatt tttatagaaa aatagtagca ttatggagaa gcaaattctt gtaattcagc
     3421 taagcgttca tgtatttttg cttgccaaat tgcttgtgat ttttcattca tgctgtgatt
     3481 ggtttctcta tcatacgcat cttgttcaat gtcccaagca tttattatag aatcacctat
     3541 tttttttagc aacgacgcat cttttgacga ttttattttt tctgtcaatt gtttacgaag
     3601 catacgcgca tataattctg aaatatcgaa atgcaattgt tcgtgttgca acaaataatc
     3661 actatacact ttcaaacttt tccatgaggc atttggataa aactttgcat atacttgcca
     3721 ctggattttt ccacccgata ttgaataatt atactgtact gacgatgctg tcatcgcttc
     3781 ttctgacgtg ccggttgctg gctttccttt gaaatcactc caacttaatt tgctatcagc
     3841 attccaatac ataaattcat catacttatc ctttttattt gatgagtgtg caaataaatt
     3901 gatgaaaagc aatattgaaa ataagaaaat gtataagcgt tgcatatttg ctgtaacgca
     3961 aaaaacgtac ctaaattata ttgaaataca ttaaatagta gttaacagca aaaattgctt
     4021 gttatatata tcaaatcgtt ggattaattc atctataaat gattgtccat attttacata
     4081 gtacgaagaa aaattttcaa cacgttcttg caatgattga tttggaaaca attgctgttt
     4141 caatttttct atttgcaata attcattttc atttttttgt tttaatgatt ttagcaagcg
     4201 tttttgaata gcgtcaatac tttgcaacgt tttttgttgt tccgcaccaa ccacatttac
     4261 caagcttaca tctattgatt gagttttgtt tttaatctca ttaaaaacct tttctatatc
     4321 gtgtttatag ttagaaaaat ctaagtccgt agctgtattt ttttcaatat atattttttt
     4381 caataggtca atatcttgaa aaaaatcttg cgttgtgaaa cctaatttca ttgctttttt
     4441 tgtcagtgca tcatttatca acaaaacaga attgcgaaca attaattgcg ggaaatttac
     4501 tttgtaatgt tggaataatg ttttcagttg tagccaataa ctaatttcgc cagcgccacc
     4561 aatgtaagcc aatgctggca atatcttttg ttgaaagagc ggacgcaaaa ttacattcgg
     4621 tgaaaagcgt tctggattat ttttcaactc gtctattatt tcattttgta agaaagtgat
     4681 attcgtatta acaacactat atgtagatgt cgaagcatta aattcaatac gctcgcgagc
     4741 atttttgaat aaataaaaca aattaattgc acgtggaaaa gcttgttgtt tatagccttt
     4801 tgaagccagt aattgcaatt gctgttctac caacgttatt gattgttgct gcaataactc
     4861 atcttgcatg acatcagaaa aagcctgttt aaaactagca tcattgccat caacaatgac
     4921 taatccatat tttccaaaga gagaatgcaa taaatatcgt gttgcatttg tcaatgaatt
     4981 ttgagcataa gcacttcgaa atagctgtat taaatcatta tcctcatcaa cgtgtaatat
     5041 tgattttatt tcatcaatta aaggcaatat actttctgga ttatagtttc cagttgcgcc
     5101 accttgttta tcttgccatt caattttttt attgtataaa tagatgtgat taatttcttc
     5161 aaaatcatgg tcttcacttc ctaaccaata aataggaaca aaactaatat ctggatattt
     5221 taattttaat tgctgacaag tagaaattgt ttgtgctatt ttataaatat aatacaatgg
     5281 gccgccaaaa atattgagtt gatgtgcagt aactatacaa tatgaattgg aattggataa
     5341 gcaattaatg ttacttgcga cagcatctga aagttcgatg ttcgcatatt gcctttgaat
     5401 tgtatgcact aaattatctc tgtcaatttt ttcattttgc ttgtctgtaa ttgcttgttg
     5461 aatagcattg atatccagag aataattata aaataagtgt gtagtttcga cgctgtttac
     5521 gtagtccaat attagcttgg aaaacatgcc tgtttcagaa aaaggaatat ttatttcttg
     5581 cactttgtga atttagaata gcaataataa acaaaatatt gtcacacaag ttcagcatat
     5641 aaatcgaaat cagtagcatc tgtaattttt gctatacaga aatcgccgat gcggcaataa
     5701 tttttattgg catcaagcaa tacttcatta tctacttctg gtgaatcgta ttctgttctt
     5761 ccaataaaat agccaccttc ttttctgtcg ataaggactt tgtaagtatt tcctattttt
     5821 tcttcatttt tcaaaaaaga aatttcttgt tgcacttgca ttagctttgc tgcacgctct
     5881 tcttttgtat ctgcgtcgat atcatcatct aattcaaaag ctgcggtatc gtcttcatgt
     5941 gaatattgaa aaacacctaa tcgctcaaat ttcatttctt ctacaaatgc acataaatca
     6001 ttgaaatcat cttctgtctc accaggaaaa ccaactaaca ttgtagtgcg aatagcaata
     6061 tttggaattt tagtacgaat gtgttgaatt aatgctttgg tttcttcttg tgtaatctgg
     6121 cgttgcatac gcttcagcac attgttattc gcatgttgta atggcaaatc taggtactta
     6181 caaactttat cacagtcttt aattgtatct aaaatatcta aaggaaattt ggaaggatag
     6241 gcataatgca aacgaatcca ttcgattcct tcgagttcat ttagtgcata taacaatttt
     6301 gaaagtgcac gttctttgta aatatctaaa ccataatacg ttaattcttg cgcaatcaac
     6361 atgatttctt taacgccttg tttagcaaga ttttttgctt ctgcaacaat gtcttctttt
     6421 gttttagaga catgttttcc acgcatgatt ggaatagcac agaatgtaca ggttctattg
     6481 cagccttccg aaatttttaa atatgcataa tgttgtggcg ttgtcaatac acgttcgcca
     6541 acaagttcgt gtttataatc tgcatttaat ttctgcaaaa gttgtggcag ttccaaagtg
     6601 ccaaaatagg catctacttg cggaatttct tgttctagat cgtctttata acgttgtgac
     6661 aggcaacctg tcacatataa tttatcaata cggcctttat ccttttgttc agcatacgaa
     6721 agaatagtat taattgactc ttctttggct ctatcaataa aaccgcaggt attgacaata
     6781 ataatattgg catctttact tgctgattga tgttctacat caaactcgtt atgttttaat
     6841 tgtgccacta acacttctga atcaacagca tttttagagc aacctaaagt gatgacatta
     6901 tacttatctt ttgtatgcga ttttgttttc atttgcagta caaaggtacg aattttggtt
     6961 ttatgcgata attaaatcag aatttcaaca accatttcac aacttcattc gcatcggcaa
     7021 ttgaccatga gtgaggttgt ttatatttta atactttgcg atatcctttt cctttagtta
     7081 atattaaagg agaattcgta tttcccaatt gctttaattc attaataaaa ccaacattat
     7141 cagccaaatt taactcatac aaattccatc ttttttcatt cattgcccaa tcaatatcag
     7201 gctcgcagta agtgagtaat ggcattttcc caaataatct tgcaccataa ttattgctgt
     7261 cttcataaca ataaggcgat aggtttacat aattttcaaa tgcatctttc aaatttcctt
     7321 tgaaaaaagt atgcatttta tccaggtaaa tggtgtatag tggtttattt gtgttatacc
     7381 attttgaggt gtcgttcttc caatgttcat atattttatg catgcgaaca aaatctaatg
     7441 gcggatcgat agtaaataca gcctttggca tgggttgaat atcatagata ttgcaatgtt
     7501 gaacatactt aatggctgat gtgccaccta aagaaaaacc accaatataa taggcatcgt
     7561 ttggaatatt gttgcgcgtt aatagtaaag gaataatgct atctaaataa tgctgtgcat
     7621 tgccatcaat agaaaaagac tgactaccat cagtattaca gataatggct gttaatatac
     7681 cttttgatgc agcaatttca ggaatattgg tttcaatttc tgcaatttgt ggcgattctc
     7741 cgaagccagt aagtagaatc aacatttttt tggctggacc attcgggcga taaaaaacag
     7801 cataattttt agcgctatct gttttatcaa aatattctat agtgcgataa taagatactg
     7861 atttttggga aaaactcagt ataaactgga ctagtaaaat aaatatgcaa aaaatttttc
     7921 tcattatttg gctttgccac tatctattat aaagtttaaa atatacatga gaaatggagt
     7981 ttgtatcaaa aggcaaaaat ttactcaaac aatgtttcga taaatacttt gcgattaaat
     8041 acttgtagtt gttccatttt ttcgcctaat ccaatatatt taattggaat tttaaattca
     8101 tcagcgatac ttaaaactac accacctttt gctgtaccat ctaattttgt cagcgccaat
     8161 gccgttactt gagtagctgc agaaaaatgt ttggcttgtt ctaatgcatt ttgaccggtt
     8221 gaagcatcta acaccaacag tacttcatga ggtgcatcag gaataagctt agacattact
     8281 tttttaattt tggaaagttc atccatcagt ccttgtttgt tatgcaagcg accagcagta
     8341 tcgataatga caacatctgc attttttgct attccacttt gaacagtgtc aaaggcgaca
     8401 gctgcaggat cagagcccat tgcttgtttt acaattggca caccaacgcg ttcactccag
     8461 atggtaagtt ggtcaacggc ggcagctcta aacgtatcag ccgcacctaa aagcacttct
     8521 ttgccttcct tttttaattg ataagctaat tttcctatcg tcgttgtttt gccaacacca
     8581 ttgactccaa ccaccatgat tacatgtggt tttttatctg tgtttggagc aaaatttaat
     8641 aagtcaacag tgttgttttc tgctaaaata ttgccaattt catctttcaa tatcttattc
     8701 agctcattcg tgttcaaata tttatctctt gacacacgag cttctaacct atcaataatt
     8761 tttatggtag tagaaagtcc gacatcagac gaaattaaga tttcttctaa ctcatctaag
     8821 aattcgatat caacagtaga ttttccggca acagctttag aaattttaga gaaaaaacct
     8881 tgttttgttt tctccaaacc ttcgttgagt tgtttttctt caattttaat ttcttcttca
     8941 gatttggttt taccaaagat tttatcaaaa attcccaatt ttcaaggttt aatggtggat
     9001 gaatagcaat caaatataaa caaaaaagcc tctcatgatg gagaagctat attattattg
     9061 atttaaaatc aattatagac ttttaacagt gtcttgcaac aagtctttgt gtactacttt
     9121 ttcacgagta gaataagcac ctgtttttgc agagcgaaca aatttgatga ttttcaccat
     9181 gttttttgat tcacctacat ttttacctct atcaactttc gcgtttttgg atactttagc
     9241 catgacttat tattttattt ctttatgaac tgttactttt ttcataatcg gattgaattt
     9301 tttcaattcg attctttcag gtgtattttt tctgttttta gttgtaatgt agcgagaaat
     9361 acctgctaaa ccagatgttt tatgctctgt acattctaaa attacctgaa ttctatttcc
     9421 tttcttcttt gccattgtag tgtcttttta gaaacctaat tgaatttcac cttttttttc
     9481 taattctttt aggtatgagt aaataccttt tttgttgatg gttctgatgg ctgaagtaga
     9541 aactttcaac tcaatccatc tgtccaattc aggaataaaa aacgcttttt tctgtaaatt
     9601 cggatagaat cttctcttcg ttttaatgtt agagtgagaa acgctatttc ccgtcattgg
     9661 accttttcct gataaatcgc aaaatcttgc catgttgtct gttttaaaga ggtgcaaata
     9721 tcgtaaaaaa aaacgagatt tcaaaacacc ttatacacaa atgttaattc ccaaaaaaat
     9781 caaaattagt gaacctgaag ggagtcgaac ccctaacctt cgcatccgta gtgcgatgct
     9841 ctatccagtt aagctacagg tccagctatt tttgagatat tttttcggaa tgcaaagata
     9901 gtatttaatt tgttattttt aaaacacgta ccgatggaaa atttattatt tatcgtttag
     9961 ttaacgaagc tagattcaat ttctgtaatt tattgatgat ttcgattttc aaaaccacac
    10021 ataccaacat ttctgaataa attatgttca aattgaccac aaaaacatat tgatgttata
    10081 atatgattaa gaaaaacagg ttgctatttt aggtattagt cgtgtcagca ataattaaac
    10141 cgtctttcat gactaatttt cgatcggctt tgtcagctaa tttatcatta tgcgtaacaa
    10201 taacaaatgt ctg
//
LOCUS       DAOQQV010000092        10188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2408_length_10188_cov_7.430080, whole genome shotgun sequence.
ACCESSION   DAOQQV010000092 DAOQQV010000000
VERSION     DAOQQV010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2408_length_10188_cov_7.430080"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1296)
                     /locus_tag="PLL59_09420"
     CDS             complement(43..1296)
                     /locus_tag="PLL59_09420"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HMY24069.1"
                     /translation="MSGNQQQETNYSLDTIECAIEAVRNGKIVIVVDDEDRENEGDFI
                     TAARNATPEIINFMATHGRGLICAPLIEDRCVELQLDLMVNSNSSHYETPFTVSVDLI
                     GHGCTTGISASDRAKTVQALINKNTKPDELGKPGHIFPLKAKKEGVLRRTGHTEAAID
                     LARLAGFEPAGLLVEILKEDGECARLNDLFPIAKKFNMPIISIKDLVEYRLQHDRLIH
                     REVEVKMPTKYGDFKLVAYKQVGSTEEHFALVKGEWKAGEPVMVRVHSSCITGDILGS
                     LRCDCGDQLHNAMKMVEKEGKGVILYMMQEGRGIGFMNKLKAYKLQEEGLDTVDANLA
                     LGFKADQRDFGIGAQILRDLNITKMKLITNNPKKRAALKGYGLEIVENIAIETVPNQH
                     NHKYLSTKRDKMGHEFTQMPKLKHD"
     gene            complement(1268..2338)
                     /locus_tag="PLL59_09425"
     CDS             complement(1268..2338)
                     /locus_tag="PLL59_09425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY24070.1"
                     /translation="MLLQHKALGKNISFTSYSLSEELPHVAIVIPVCNEEKVIKEKLD
                     SIFNCSYPLDKLHVYIGLDNCTDASKSIITTNFNFPNLHIIEFTERQGKPSVLNKIIA
                     NNVTAEHHLLILTDANVIFNHNTIFELVKYFKESSIGLVDGNIQAKNIDNRHEFDYWN
                     YETLIKINESKLYSKILGPSGACYAIRKSLFNPIPNNFLVDDFYIGFSIITQQNNAII
                     NVDATCVEVSNTNWKQEFNRKVRIAAGNFQNLWFFKKYAFNIFSAIGFMFVSHKVIRW
                     KTPFLLLILYYILLLEFTLFVLIVTFTLPIIDTVFRIFGVEFKPLRQFHYFIVMNIAV
                     FIGFLKFCKGIKSNVWQPTTRN"
     gene            2518..3477
                     /gene="fmt"
                     /locus_tag="PLL59_09430"
     CDS             2518..3477
                     /gene="fmt"
                     /locus_tag="PLL59_09430"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HMY24071.1"
                     /translation="MKKQLRIVFMGTPDFAVPSLAALLDNKYQVVGVITAPDKPAGRG
                     QQIQQSAVKKYALENELNILQPEKLKSPDFINELQNLNANLFIVVAFRMLPEIIWQMP
                     TFGTFNLHASLLPQYRGAAPINWAVINGENETGVTTFFLQHEIDTGNIIFQEKIKIEA
                     TDNAGNVHDKLMHVGSSLVLKTVKAIEEDTVIQQTQSSIESGFLRHAPKIYKETCLID
                     WNKSVTEIHNLIRGLSPFPTAFTYIDGKVLKIFEARQFNNEITETIDVESKNDLQISI
                     ITNNKTYLAIKCSDGFLELLTVQLEGKKRMSIEEFLRGYKFIS"
     gene            3736..4593
                     /locus_tag="PLL59_09435"
     CDS             3736..4593
                     /locus_tag="PLL59_09435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMY24072.1"
                     /translation="MAENNSNKPDAKIRKVNFKFEKPFPKHWYNDNPIATHFMNAQHL
                     AFPDGEKFFIRSVKAFADTYKNDPELKRRVDNFIGQEGTHFAEHQKFWDIMEDQNLQP
                     MKFVNVYRNSAWEGIEKWARKKFVKNNVGNKIALSVTVALEHYTAMLAESGILNEDIT
                     EKMPEEMKNLFMWHAAEEIEHKSIPFDVLKKVDDSYALRIGGMVLATWGLWFYLGLGT
                     VMLTHNDKDIKTKDIPKHTFTFLKNFRRTFGGTLASQFGQYFKRDFHPDQIDNYHLAV
                     ELLKGKNYA"
     gene            4632..5516
                     /locus_tag="PLL59_09440"
     CDS             4632..5516
                     /locus_tag="PLL59_09440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMY24073.1"
                     /translation="MATTTAKKAEKVEKKAYDPEEIKVRRVNFQFKKDTSKFYYKNNP
                     FSTSFINVLHILFPTGERFFVNSVLKHQKSIKDEKLKKQVRNFCGQEGIHSSMHERFW
                     TILKDNGYIIEGYENHIDKLLHKLVAKIELEGTKINNIDLVATTCLEHFTALFGHAIF
                     ARVDVTPSAAPQELAELFQWHAAEEIEHKSVAFDVLQQVDPDEYAKRIIAMPVATAIL
                     YFYLTVGTLMLLYQDRKDLQITKLPKQFYEFATGLFMELHGDVFKEYFSFFKKGFHPS
                     DLNDYHYAEEFFKDKAYA"
     gene            complement(5608..7239)
                     /locus_tag="PLL59_09445"
     CDS             complement(5608..7239)
                     /locus_tag="PLL59_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HMY24074.1"
                     /translation="MKHLFFIPSMLMVLFANAININDSKITDVTVFRNYAKETRMASA
                     QIPEGNSEVIISNVSTYLDENSLQIATKGNVKILSVSSKLNYLIDKTKSSQVIKLTDS
                     IEMMNDDVSWWNQQSIAFQDELKLLDENRKLANDKSTFTAAQVKELAELYRNRALELR
                     KYIFDLNKKQKLNNDKIAKLKSQIAVLNGNQNMTVKEIILKINAKSAGLVSFKCSYIV
                     SNAYWTPIYDIRAKNNNSPLQLDCRAKVVQNTGYDWKDVRLTLSTANPSANHNRPVLY
                     PIYVDFFQPDYYEYKLKTSSYGEAAQMMNMAAPAKIDIARSTADDEFEEGLKATDNNV
                     TIQEGDMAVEYNIETLQDVESDNKEHIVAIQEIEMPALYAYHTVPKLDMTAYLLARVT
                     DWSKYNLLAGEANIFFDDNYVGKSYLNPNVSADTLLISLGRDEKINIKRVKLNDYSAK
                     KLLSGNIKETKAFETTIKNNKNTSIEIELLDQFPISKNSQIEVTLEEANGATITEEYG
                     KLVWKIKLQANESKKVKLIYTIKYPKDKQIKEKNS"
     gene            complement(7405..9033)
                     /locus_tag="PLL59_09450"
     CDS             complement(7405..9033)
                     /locus_tag="PLL59_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMY24075.1"
                     /translation="MSNKLFQSYKQKPIIDEHYDVICIGSGLGSLCTASLLARRGKRV
                     LICEKHTTPGGFTHVFTRNDYEWDVGLHYVGDMDRKGSLVRLAFEHVTDGNLKWADMG
                     EVYDKVIIEDRVYDYHKGLKAWKENMKSYFPTENDARAIDKYVELIFAVNKGAKDFYA
                     KKVMPDFLRMLASPFMNNDYSKYAHQTTKQVLDEISDNDELKAVLSAQFGDYGTPPSK
                     SSFVIHAAVALHYMNGGFYPVGGSEEIFNTIAPSIINAGGTILIGAAVKEIIVENGVA
                     KGVLMQDGKTIKSDIVVSGAGVHITYKHLLSAESKKKIKYLDDALNLPSSYGHLSLYI
                     GLKNTADELNLPKANYWIYPNGTDHDKALENYLQDYNNEFPVVYISFPASKNPNFTDK
                     YPGRSTIEIITVAKYDWFKEWENTRWKKRGEAYDVLKEKLAKRLLEHLYKYEPQIRGK
                     IDTYELSTPITTKHFCNYLQGEIYGLDHQPSRFAKEYLKPQTPIKNLYLTGQDIVTVG
                     IAGALLSGILTASAITKTNLINEMIKQKTQNNNS"
     gene            complement(9030..9797)
                     /locus_tag="PLL59_09455"
     CDS             complement(9030..9797)
                     /locus_tag="PLL59_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24076.1"
                     /translation="MSASNNKETEIQKLLHVLQVDSDTSFSFLKNLSKEELQKLRLQV
                     IEVSQFTQTDIWKRLTGVSKYFPNYMNAKISETVMGPLIVANMSYYMPIKDAIAIMKH
                     LSMPFLVSVSEYMTPEKSKNLINQIPIDILKKITTELVNRKKFMTAAGFVDVLELSRL
                     IELSKVITREDDLISISSFVENKKYIAQIIEGFTDERLSKIITVAYNFGKQDEILTVF
                     SHQSNKEVQRIMRIVSVLPVQLKTQILQDFETRVQKL"
     gene            9843..>10188
                     /locus_tag="PLL59_09460"
     CDS             9843..>10188
                     /locus_tag="PLL59_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HMY24077.1"
                     /translation="MNVFESKIICKTFQGLEQVLAEELIALQAKNVEVIKRAVVCDYD
                     LEILYKCNIALRTCLKVLVPVFEFESNSPEDLYGFAIRIPWENYLDIHQSFAIDFTIQ
                     SEYFKHSQFAALK"
BASE COUNT         3403 a         1711 c         1585 g         3489 t
ORIGIN      
        1 ctcaatgcaa cgcatgcatt gatttcattc taattcaaat gattaatcgt gttttagctt
       61 aggcatttgt gtaaactcat gtcccatttt atcgcgtttt gtactaaggt atttgtgatt
      121 atgttgattg ggaacagttt cgatggcaat attttcaaca atttctaagc catagccttt
      181 taatgctgca cgttttttag gattgttcgt aatcaatttc attttcgtga tatttaaatc
      241 acgtaatatt tgagcgccaa taccaaaatc gcgttggtcg gctttgaaac caagcgccaa
      301 atttgcatca acagtatcta agccttcttc ttgtagctta taggctttaa gtttattcat
      361 aaaaccaata cctctgcctt cttgcatcat atataaaata acacctttgc cttctttttc
      421 taccattttc atggcgttat gcaattgatc gccacaatca cagcgcaaag agcctaaaat
      481 atcaccagtt atacaagatg aatgcacacg taccattact ggttctcctg ctttccactc
      541 tccttttacc aaagcaaaat gttcttcagt agagccaact tgtttatagg ctactaattt
      601 gaaatcgcca tattttgtag gcattttcac ttcaacttct ctatgaataa gtctgtcgtg
      661 ttgtaagcga tattctacta aatctttaat agaaataatc ggcatattga attttttggc
      721 aattggaaat aaatcattta aacgcgcaca ttcaccatct tctttcaata tttctacgag
      781 caatccagct ggttcaaaac cagctaatcg agccaagtct attgctgctt ctgtgtgacc
      841 agtacgtcgt aaaacacctt ctttttttgc ttttaatgga aaaatatggc ctggctttcc
      901 caactcatct ggtttagtgt ttttgttgat taaggcttgc accgttttag ctctatctga
      961 tgcagaaatg ccagtagtgc agccatgtcc aattaaatca acagaaactg taaatggcgt
     1021 ttcataatgt gatgaattag aattaaccat caggtccaat tgcaattcta cgcatctatc
     1081 ttcaattaat ggtgcacaaa taagtcctcg accatgcgtg gccatgaagt taattatttc
     1141 aggtgttgca ttgcgtgcag ccgtaataaa atctccttcg ttttcacgat cttcgtcgtc
     1201 aacgacgatt actatttttc cgtttctaac ggcttcgata gcgcattcaa tggtatctaa
     1261 gctgtagtta gtttcttgtt gttggttgcc agacatttga tttaattcct ttacaaaatt
     1321 ttaaaaatcc aataaaaacg gcaatgttca tgactataaa ataatgaaat tgacgtaatg
     1381 gtttaaattc cactccaaaa atacggaaaa ctgtatcaat tattggtaaa gtaaaggtta
     1441 cgattaaaac aaacaatgta aattctaaca ataaaatgta gtataaaatc aataaaagaa
     1501 atggcgtttt ccatctgatt actttatgag aaacaaacat aaaaccaatt gcagaaaaaa
     1561 tgttgaaagc atatttttta aaaaaccata aattttgaaa gtttcctgct gcaatgcgta
     1621 cttttctgtt aaattcttgc ttccaatttg tgttgcttac ttctacacat gtcgcatcaa
     1681 cattaataat tgcattattt tgttgtgtaa taatggaaaa accaatataa aaatcgtcca
     1741 ctaaaaaatt attgggaatt ggattaaaca atgattttct aatcgcataa caagcacctg
     1801 aagggcctaa aattttacta tataatttac tttcattgat ttttattaaa gtttcataat
     1861 tccaataatc aaactcatgt cgattgtcaa tatttttagc ttgaatgttt ccatcaacaa
     1921 gtccaatact actttcttta aaatatttta ctaattcgaa aatagtgtta tgattaaaga
     1981 tgacattagc atccgttaag attaataaat gatgttcagc tgtaacatta tttgcaatta
     2041 ttttattgag aacacttggt tttccttgtc tctccgtaaa ttcaatgata tgtaaatttg
     2101 ggaaattgaa gttcgtagta ataatcgatt ttgatgcatc agtgcaatta tccaagccaa
     2161 tataaacatg caatttatct aaaggatagc tgcaattaaa tatactatcg agtttttctt
     2221 ttatcacctt ttcttcattg caaacaggaa taacaatagc aacatgtggt aattcttcag
     2281 aaagtgaata agaagtaaat gaaatatttt tgcctaatgc tttatgttgc aataacatcg
     2341 gataaaagat gtaggcatac aataacaaaa ataatgttag caaaaacaca atccaaatca
     2401 agcgagaaag tttatacgaa attacagaaa gattattgct tttaataaga aatttatcaa
     2461 gcagggcttg cttgaatttt cgacaatata tccatttcct tattgtattt ttgttgcatg
     2521 aaaaaacagt tgcgcattgt atttatggga acaccagatt ttgcggtgcc gtcattagct
     2581 gcattattgg ataataagta ccaagtagtt ggcgttatta ctgcacctga caaaccagct
     2641 ggacgcggac agcaaataca acaatctgct gtgaaaaaat atgcccttga aaatgaatta
     2701 aatattttac aaccagaaaa gctgaaaagt cctgatttta ttaatgaatt gcagaattta
     2761 aatgctaatt tatttattgt tgttgctttt agaatgctgc ctgagataat atggcagatg
     2821 ccaacattcg gcacgttcaa ccttcatgct tccttgttgc cacaatacag aggcgcagca
     2881 cctatcaatt gggcagttat taatggtgaa aatgaaacag gtgtgacaac attttttcta
     2941 cagcatgaaa tcgatacagg taacattatt tttcaagaaa aaataaaaat agaagcaaca
     3001 gataatgccg gaaatgtgca tgataaacta atgcatgtcg gttcttcttt agtgctaaaa
     3061 acagtaaaag ccattgaaga agatacggtg atacagcaaa cacaatcaag catagaaagt
     3121 ggatttttac gacacgcacc taagatatat aaagaaacct gtttaatcga ctggaataaa
     3181 tctgtaacgg aaattcataa tttaattcgt ggactttctc cttttccaac ggcattcaca
     3241 tatatagatg gaaaagtatt gaaaatattt gaagcaaggc aattcaacaa tgaaattacg
     3301 gaaacaatcg atgtggaaag taaaaacgat ttacaaataa gtataataac aaacaacaaa
     3361 acctatttag caataaaatg ttcagatggt tttttagagt tactaacagt acaactcgaa
     3421 ggaaagaaaa gaatgtctat tgaagaattc ctaagaggtt ataaatttat atcttaggaa
     3481 tttttaataa aaatacacac ttaaatacat tcaactaaaa taacacaagt attcatcgaa
     3541 tataatagtc agaagtatta aaatctcaat attttataca aaatttcgtc ataaagcacg
     3601 tattatcgtg tgtattaaat tgatattaag aagaaattaa taaacaagtg ttcataatca
     3661 aaaaactcat tcttatctat tgtataaagc aaggtatgat tatctgtttt aattttagta
     3721 tagttaataa aacaaatggc agaaaacaat agcaataagc cagatgcaaa aatacgaaag
     3781 gtaaatttca aatttgaaaa accatttcct aaacattggt ataatgacaa tccaattgct
     3841 acacatttta tgaatgcgca acatttagca tttcctgatg gtgaaaagtt ttttattaga
     3901 agtgtgaaag catttgcaga tacatacaaa aatgatccag aactcaaacg acgtgtagat
     3961 aatttcattg gacaagaagg tacacatttt gcagagcatc aaaaattttg ggacattatg
     4021 gaagaccaaa atttacagcc gatgaaattt gtaaatgtgt atagaaactc agcttgggaa
     4081 ggcattgaaa aatgggcacg taaaaagttt gtaaaaaata atgtaggcaa taaaattgca
     4141 ttgagtgtta ctgttgcttt agaacattac actgcaatgc ttgcagaaag tggcatttta
     4201 aatgaagaca ttactgaaaa aatgccggaa gaaatgaaaa atttattcat gtggcatgca
     4261 gccgaagaaa tcgaacataa atcaattcct ttcgatgtgc taaaaaaagt agatgatagc
     4321 tatgccttgc gtatcggagg catggtgcta gccacttggg gcctttggtt ttacttaggt
     4381 ttaggcacag ttatgttgac acataatgac aaagacatta aaacaaaaga tatacctaaa
     4441 cacactttta catttttgaa aaactttaga cgaacttttg gaggtacatt agcttcacag
     4501 tttggtcaat attttaaaag agattttcat cctgatcaaa tcgataatta tcatcttgct
     4561 gtagaattat taaaaggcaa gaattatgca taacacaaac aaaacaacat ttttcaatta
     4621 aacaaacaat tatggcaact acaacagcaa aaaaggcgga gaaagtagaa aaaaaagcgt
     4681 acgatccaga agaaatcaaa gtgcgccgtg tcaattttca gttcaaaaaa gacacatcaa
     4741 aattttacta taaaaacaat ccgtttagca caagctttat taatgtattg catatcttat
     4801 ttccaacagg cgaacgcttc tttgtgaatt cggtattgaa gcatcaaaaa agcattaaag
     4861 acgagaagtt aaaaaaacaa gtgcgtaatt tctgtggtca agaaggcata cacagtagca
     4921 tgcatgagcg tttctggaca atcttgaaag ataacggtta tattatcgaa ggttacgaaa
     4981 accatatcga taaattattg cataaattag tggcaaaaat agaattggaa ggcactaaaa
     5041 tcaataatat cgacttagtg gcaacaacat gtttagaaca ttttacagct ttatttggac
     5101 atgccatttt tgcacgtgta gatgtaacgc caagcgcggc tccgcaagaa ttagcagaac
     5161 tattccaatg gcatgcagca gaagaaatag aacacaaaag cgtagcattt gacgtgttgc
     5221 aacaagttga tccagatgaa tatgcaaaac gtattattgc aatgccagtt gcaacagcaa
     5281 tattgtattt ctatttaact gtaggcactt taatgctttt atatcaagat agaaaagatt
     5341 tacaaatcac aaaattgcca aaacaattct atgaatttgc gacaggatta tttatggaat
     5401 tacatggcga tgtgtttaaa gaatatttca gcttcttcaa aaaaggattc catccttcag
     5461 acttgaatga ttatcattat gctgaagaat tttttaaaga taaagcatac gcttaatcat
     5521 tgaaaataga taaaaaaatg aagaagcggt cacctacggt gaccgcttct ctttttatac
     5581 agcaacaacg gcatttgtgc tgtatattta actgtttttt tcctttattt gcttgtcttt
     5641 tggatattta attgtatata ttaatttcac ttttttagat tcgttggctt gtaattttat
     5701 tttccaaact aacttgccat attcttccgt aatcgttgcg ccattggcct cttccaacgt
     5761 cacttcaatt tgagagtttt tagaaattgg aaattgatcg agcaattcaa tttcaattga
     5821 tgtattctta ttgtttttta tcgtggtctc aaaagccttg gtttctttaa tatttccact
     5881 caataatttt tttgctgaat aatcatttag cttaacacgt ttgatattta ttttctcatc
     5941 acgacctaat gatattaaca aagtgtcagc acttacattc ggattcaaat aagatttacc
     6001 gacataatta tcgtcgaaaa aaatattggc ttcacctgct aataaattat atttcgacca
     6061 atccgttaca cgcgccaaca agtatgccgt catgtctaac ttaggtacag tatgatatgc
     6121 atacaacgca ggcatttcaa tttcttgtat agcaacaata tgttctttat tgtcactttc
     6181 cacatcttgc aaggtttcaa tattatattc gacagccata tcaccttctt gtatcgttac
     6241 attgttatct gtagctttta agccttcttc aaattcatca tcagctgttg aacgtgcaat
     6301 atctattttt gctggcgctg ccatattcat catttgcgcg gcttcgccat aactacttgt
     6361 ttttaattta tattcgtaat aatcaggttg gaaaaagtca acataaattg gatacaaaac
     6421 aggtctattg tggttggcag atggatttgc agtagataat gtcaatctca catctttcca
     6481 atcgtagcct gtattttgca caacttttgc tctgcaatct aactgtaatg gcgaattgtt
     6541 attcttggct ctgatatcat aaatcggtgt ccaatatgca ttgctaacaa tatatgaaca
     6601 tttaaacgaa accagtcctg cagattttgc attgattttc aaaattattt ctttgactgt
     6661 catattctga ttgccattta acacagctat ttggcttttt aattttgcta ttttatcatt
     6721 gttcagtttt tgttttttat ttaaatcaaa aatgtattta cgtaactcaa gagcacgatt
     6781 gcgatataat tcagctaatt cttttacttg ggccgcagtg aacgtgcttt tgtcattcgc
     6841 taattttcta ttttcatcta atagctttaa ttcatcttga aaggcaatag attgttgatt
     6901 ccaccaactt acatcatcat tcatcatttc aatagaatca gtaagtttta ttacttgtga
     6961 cgattttgtt ttatctatta aataattaag ttttgatgac acagacaaaa ttttcacgtt
     7021 tcctttagta gcaatttgca atgaattttc atctaagtaa gtggacacat tagagatgat
     7081 gacttcgcta ttgccttcag gaatttgggc acttgccatg cgcgtttctt ttgcataatt
     7141 tctaaaaaca gtaacgtctg ttattttaga atcattgata ttaatcgcat tagcaaacaa
     7201 taccattaac attgacggaa taaaaaatag atgcttcata ttgatattta ttttcttgaa
     7261 gcaaatttga agctaaaaat aaaatttaga gagaaagcta ttgtttgtat ggcaatgcta
     7321 atgtaattaa atacaataca acaaaatggt tatcaaaaaa ttaagttaag catgtatggt
     7381 atttaataat gaaaaaaaat ggcgttaaga attgttattt tgtgtttttt gttttatcat
     7441 ttcattgata agattcgttt ttgtgattgc agaagctgtt aaaataccac tcaataatgc
     7501 gccagcaata ccaactgtaa caatatcttg accagtcaaa tacaaatttt taattggtgt
     7561 ttgtggtttc aaatattctt ttgcaaaacg cgatggttga tgatctaagc catatatttc
     7621 accttgtaga taattgcaaa aatgtttagt agtaattggt gttgaaagct cataagtatc
     7681 aatttttccg cgtatttgtg gttcatattt atataagtgt tcgagcaacc tttttgccaa
     7741 tttttctttt agcacatcat acgcttcacc acgttttttc caacgagtat tttcccattc
     7801 tttaaaccaa tcatattttg ccaccgttat aatttctata gttgagcgac caggatattt
     7861 gtctgtaaaa tttggatttt tagaggctgg aaatgaaata taaactactg gaaattcatt
     7921 attataatct tgcaaataat tttctaatgc tttatcatgg tcagtaccat ttggataaat
     7981 ccaataattt gcttttggaa gatttaattc atctgccgta ttcttcaaac caatatataa
     8041 gcttaaatgg ccatacgatg atggtaaatt caaagcatca tctaagtatt ttattttctt
     8101 tttactttct gcagataata aatgcttata tgtaatatgt acacctgcac cactcacaac
     8161 aatatcgctt ttaattgttt taccatcttg catcaatacg ccttttgcga cgccattttc
     8221 cacgattatt tcttttactg ctgcaccaat taatatagtg cctccagcat ttatgatgct
     8281 cggcgcaatg gtattaaaaa tttcttcaga gccacctaca ggataaaaac cgccattcat
     8341 ataatgcaag gcaacagctg catgtataac aaaactgctt tttgatggtg gcgtgccata
     8401 atcgccaaac tgtgccgata aaacagcttt caattcatca ttatcgctta tttcgtcaag
     8461 tacctgtttt gttgtttgat gagcatattt tgaataatca ttattcataa aaggcgaagc
     8521 caacatacgc agaaaatctg gcattacttt cttagcataa aagtcttttg ctcctttgtt
     8581 tactgcaaaa ataagttcaa catatttatc aattgcacgt gcatcatttt ccgttggaaa
     8641 ataagatttc atgttttctt tccaagcttt taatccttta tgataatcat atactctatc
     8701 ctcgataatc actttatcat acacttcgcc catgtctgcc catttcagat ttccatctgt
     8761 gacgtgttca aaagccaatc gcactaaaga gcctttccta tccatatcgc caacataatg
     8821 caaaccgacg tcccattcat aatcattacg tgtaaacaca tgtgtgaagc cgccaggtgt
     8881 tgtgtgtttt tcgcatatta aaactcgctt ccctcttctt gccaataatg atgctgtgca
     8941 caagctacct aagccagacc cgatgcaaat aacatcgtag tgttcgtcaa tgattggttt
     9001 ttgtttgtac gattgaaaaa gtttattact catagtttct gtactcttgt ttcaaaatct
     9061 tgtaagattt gggtttttag ttgtactggc aatacagaaa caatgcgcat tatacgttgt
     9121 acttctttat tgctttgatg ggaaaaaaca gtcaatattt catcttgttt tccaaagtta
     9181 taggcgacag taataatttt tgacaagcgc tcatcagtga atccttctat aatttgggca
     9241 atgtattttt tattttctac aaacgaagaa atcgaaatta aatcatcttc acgtgtaatt
     9301 actttagaca actctattaa tctagaaagc tccaaaacat caacaaatcc agctgctgtc
     9361 atgaatttct ttctattgac taattctgtg gtaatctttt ttaatatatc aattggaatt
     9421 tgattaatca agttttttga tttctctggt gtcatatatt cagaaacact cactaagaat
     9481 ggcatactta aatgtttcat gatggcaatc gcatctttta ttggcatata gtagctcatg
     9541 ttcgcaacaa ttaatggacc cattactgtt tcagaaatct tggcattcat atagtttgga
     9601 aaatatttcg acacaccagt tagtcttttc caaatatccg tttgtgtgaa ctgggaaact
     9661 tcaataacct gcaaacgtag cttttgtagc tcttcttttg ataaattttt taaaaatgaa
     9721 aatgatgtat cactatctac ttgtaaaaca tgcaatagct tttgtatttc tgtttcttta
     9781 ttatttgaag cactcatact ataaatataa aaaatgattt tataaaattg tatttttgtg
     9841 ctgtgaatgt atttgaatca aaaattatat gtaaaacttt tcaaggctta gaacaagttt
     9901 tggcagagga actaattgca ttacaagcaa aaaatgtaga agtcatcaaa cgcgctgtcg
     9961 tttgcgatta cgacttagaa atattatata aatgcaatat tgctttacga acttgtttaa
    10021 aagtattagt acctgttttt gaatttgaat ccaattcacc tgaagatttg tatggctttg
    10081 cgataagaat tccatgggaa aattatttgg acatccatca gagctttgcc atcgatttta
    10141 ctatacaatc agaatatttc aaacattctc agtttgctgc acttaaat
//
LOCUS       DAOQQV010000093         9998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2483_length_9998_cov_8.862013, whole genome shotgun sequence.
ACCESSION   DAOQQV010000093 DAOQQV010000000
VERSION     DAOQQV010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2483_length_9998_cov_8.862013"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..209)
                     /locus_tag="PLL59_09465"
                     /pseudo
     CDS             complement(1..209)
                     /locus_tag="PLL59_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
     gene            complement(163..1497)
                     /locus_tag="PLL59_09470"
     CDS             complement(163..1497)
                     /locus_tag="PLL59_09470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HMY24078.1"
                     /translation="MSVQAISISEALKEYFGFEKFKGNQQQIIESIIARKDTLVIMPT
                     GGGKSLCYQLPAIISEGTAIIVSPLIALMKNQVDLVRGYSSTDNIAHFINSQMNKAQI
                     TQVKKDIVSGKCKLLYVAPESLVKQDNIDFLQTIKISFVAVDEAHCISEWGHDFRPEY
                     RKIKSMLNEIEDNIPIVALTATATPKVQSDIVRTLGMSAPKIFISSFLRDNLYYEVRP
                     KPSKQDAIKDIIKYIKKEGNKSGIIYCLSRNSTEELAEALQVNGINAAAYHAGLDAAV
                     RSERQDQFLMEDVQVIVATIAFGMGIDKPDVRFVIHFDIPKSLENYYQETGRAGRDGL
                     EGNCIAYFSPKEIHKFEKFILSNKEKTVAEKEIAVMQLSEVEAYSESAECRKKFVLHY
                     FGEDMKNCGRCDNCLHPKEQIEAKDEIKLALQTVTDTNHFYGNRPGCFLSAN"
     gene            1719..2801
                     /locus_tag="PLL59_09475"
     CDS             1719..2801
                     /locus_tag="PLL59_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HMY24079.1"
                     /translation="MKTNTFVCIMAGGIGSRFWPASRTAKSKQFLDILGTGRTLLQST
                     FDRFLKICPKENIFILTHTDYINLVKEQLPDITDIQILTEPARKNTAPCIAYAAFKIN
                     KINPDANIIVAPSDHIILNEEEFVRITELALDFVALNDALCTLGIRPTRPDTGYGYIQ
                     YFEEESAEGIHKVKTFTEKPIKDVAIQFVKSGDFLWNAGIFIWSAKSILTALEKNLLE
                     VYDAFVEAEPHILTDKEKTAVEEAFITCPSISIDYGIMEKAKNVYTLPSSFGWSDLGT
                     WASLYVEKDKDYFHNAVNGKNVVVYDSTNNIINVPEKKLVVIQGLENFIVVDTDDVLL
                     ICNKDEEQRIKEFTHDIKMKKGDKYL"
     gene            2900..4381
                     /locus_tag="PLL59_09480"
     CDS             2900..4381
                     /locus_tag="PLL59_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMY24080.1"
                     /translation="MSKLFAKKSISTLLQEAEDSEKGLKRTLNSWALIALGIGAIIGA
                     GLFVRTAAAAAQNAGPSVTIGFIVAAIGCALAGLCYAELSSSIPISGSAYTYTYATMG
                     ELLAWVIGWDLVLEYAVGAATVGIAWSEYLNNFLTQILHLAPIPYQWCHSPFQTSVDG
                     VSGIVNLPALGIVSIISLLLIKGIQESAFVNNIIVILKVSIVVLIIALGWGFINPENH
                     TPYIPAATQYTDHDGITYHFGGIMGILGAAGTVFFAFIGFDAVSTAAQETKNPSKSMP
                     IGILGSLAICTVLYILFAHVLTGLESVDFFRDPNKGGEASVSAAIQSAMPGYSWLSKS
                     VTIAILAGFSSVILVLLLGQSRVFYSMSKDGLLPKVFSDIHPTYKTPYKGNLAILVLV
                     AAFAGFVPGNVVGHMTSIGTLFAFMLVCIAVIVLRKTEPNMPRQFKTPFVPVVPILGV
                     IACGAMIFGLGAENWIRLIVWLIIGFVIYFGYSKKHSKVNNPK"
     gene            complement(4447..5973)
                     /locus_tag="PLL59_09485"
     CDS             complement(4447..5973)
                     /locus_tag="PLL59_09485"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMY24081.1"
                     /translation="MRITGIKARPETAKQLYPYQEEYIKRIFQHLEENERRENIIFQL
                     PTGGGKTVIFSEIARKYIEQNQKKILILTHRIELLKQTANAIEEAGIHCKLITSEVDD
                     IPDQEEFLCFLAMVETLNNRLKEDEEYLQDIDFVIVDEAHYNSFRKIFHYFRNAVILG
                     VTATPLSSNQNLPLAQNYKRLIIGDSIKELISKGYLSNAITYTYDVHLGGLKVGIDGD
                     YTVSSLDRIYMHFDMHEKLLRAYQEKAKNKKTLIFNSSVATSKSVEKFFLDQGVPIRH
                     LDSTSNKQDRKDVLQWLKETPDAIVTSVGILTTGFDEPTVECIVLNRATRSLTLYHQM
                     IGRGSRKLPSKSEFMVIDLGNNARRLGLWQDYIDWRDVFVNPEKFLEHLYEREIQMEK
                     GLLYDLPDTVKALFPNTTDFDFDMEKVYYSLYNKGKKTLESIDISIENHFERIRANTT
                     NYLDALPLINALQDEIQYRLNVYISCLSKATKNYFNYLLESYNEKLHNKLKSALPFSE
                     "
     gene            complement(6135..7031)
                     /locus_tag="PLL59_09490"
     CDS             complement(6135..7031)
                     /locus_tag="PLL59_09490"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HMY24082.1"
                     /translation="MTQTFHANGKLLLSAEYFILDGAVGLALPTKFGQSLKVEDSSEN
                     GLTWESFDHKNEIWFSAKFDEQFTILHTTNVAVAEILQVLLQKSNCTHHNAHITTKLD
                     FPNHWGLGSSSTLISLIAQWTNANPYELLQQTFGGSAYDIACATAKNAISYQLTSQNT
                     RQITALDFNPAFKENIYFVHLGKKQNSREGIQLYKNSHIDKTAIIEQINELTQKIILA
                     IDLDEFCYYLEVHENIIANALNLQKVKETFFQDFPGTIKSLGAWGGDFIMVCSDEKQE
                     MITHYFQNKGFSTIIPYNAMIL"
     gene            complement(7059..8378)
                     /locus_tag="PLL59_09495"
     CDS             complement(7059..8378)
                     /locus_tag="PLL59_09495"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HMY24083.1"
                     /translation="MKKISGYSKLSKEQKINWLVENYLNNDETAKSELISYWHRDLHT
                     QKILDEFSENTITNYFFPFSVAPNFLINGEWYCLPMAIEESSVVAAMSNAAKYWSERG
                     GFTTEILGTSKVGQVHFIYKGDEAQLKADFSTIKNQLLEGSKYISYNMEQRGGGVVDI
                     ELVDMTQHEAGYFQLKATFETCDSMGANFINTCLEEYASTLKELVGNNPKYGTVKIIL
                     AIVSNYTPNCIARATVSCKVEDLGSFGTISATEFAEKMRLAVRMAEVDPHRATTHNKG
                     IYNGVDAVIIATGNDFRAVEACGHTYAARNGTYGSLTHCTIENGIFKFWIDLPLAVGT
                     VGGLTQLHPLVKRSLEILGNPNAEQLMAFCAVAGLAQNFAALKAMTTVGIQQGHMKMH
                     LLNILNYYHATASEKDYALEYFKDKVVSFTTTRVMLEQLRNAKNGVK"
     gene            complement(8384..9415)
                     /locus_tag="PLL59_09500"
     CDS             complement(8384..9415)
                     /locus_tag="PLL59_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24084.1"
                     /translation="MENLNKISTDPTAATRKQDHIHLAFQSQVENALLDPRFSYEPIL
                     SKHPDEALPSTRFLGKTMAAPIWVSSMTGGTEKAYQINANLARACKQFGMGMGLGSCR
                     ALLESDERLKDFNFRNIIGDTLPFYINIGIAQLEALLQNNKLYQIDQLIDKLRADGII
                     IHVNPLQEWLQPEGDKITQAPIITIQQLLDTTTQKVIVKEVGQGFGKESLRALLQLPI
                     EALEFAAHGGTNFSMIELLRSDKQKMEAYQPITKIGHSATEMTMYVNELQNELGDKIL
                     CKQYIISGGIKNFLDAYYLMEKINATAVYGQASALLKYATSSYEELEQYLHFQIEGLK
                     LAKAFLKVK"
     gene            complement(9621..>9998)
                     /locus_tag="PLL59_09505"
     CDS             complement(9621..>9998)
                     /locus_tag="PLL59_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24085.1"
                     /translation="LQAKNEYRDVYFILVDHGPKPGAQGFELMCDSIYNQLKRNLKEP
                     NIEAKDTLFTTVNQFKVKEYQLSGILSSEKQDHRFQFVIDVFEAPNGHIYQTAGWLLR
                     TKRDLWLKDLQTAAYSLQTKSTK"
BASE COUNT         2886 a         1797 c         1828 g         3487 t
ORIGIN      
        1 atggaagatt catccacatt ttccttcagc aggttttgtt tgcttaattc ggtaggcgaa
       61 aaattgtatt ttgcaatccg gccggtgagt tgtctgttga gttcttcggg ccaggggctg
      121 tagagattgt gagttcgaag tccggcttca acgtgtccta catcaattcg ctgataaaaa
      181 gcagccaggg cggttgccat agaagtggtt ggtatcagtc actgtttgta aggcaagttt
      241 tatttcatct tttgcttcaa tttgttcttt tggatgtaaa caattatcgc atctgccaca
      301 gtttttcatg tcttcaccga agtaatgtaa cacaaatttt ttcctacatt cagcgctctc
      361 actataggct tctacttcgc tcaattgcat tacggcgatt tctttttctg ctactgtttt
      421 ttctttattg gataaaataa atttctcaaa cttatgtatt tcttttggcg agaaataggc
      481 aatgcaattt ccctccaaac catcgcggcc ggcacggcct gtttcttgat aataattctc
      541 caaagatttt ggaatatcaa aatgaatgac aaagcgaaca tctggtttgt cgatgcccat
      601 tccaaatgca attgttgcca caatcacctg cacatcttcc attaaaaact gatcttgtcg
      661 ctcactacgc actgcggcat ctaagccggc atgatatgct gctgcattaa tcccattgac
      721 ctgtaatgct tctgccaatt cttcagttga gtttctgctc aggcaataaa taattcctga
      781 tttatttcct tcttttttga tgtattttat aatgtctttg atggcatctt gcttgcttgg
      841 tttcggacgc acttcgtaat ataaattatc tcgcaagaaa gaagaaataa atatttttgg
      901 tgcggacatg cctaaagtac gcacaatatc agattgtact tttggcgtag ctgttgctgt
      961 taatgctaca attggaatat tgtcttcaat ttcattgagc atgcttttta tcttgcgata
     1021 ttctgggcga aaatcgtgtc cccattcgga aatacaatgt gcctcatcaa cggcaacaaa
     1081 tgaaattttt atcgtctgta aaaaatcaat attatcttgt ttaaccaatg attctggtgc
     1141 aacgtacaat aatttacatt taccactgac gatatccttc tttacttgtg tgatttgcgc
     1201 tttattcatt tgtgaattaa tgaaatgcgc aatattgtct gtgctgctat aaccacgcac
     1261 taaatctacc tggtttttca ttaaagctat caatggagaa actataattg ccgttccttc
     1321 agaaattatt gctggcaatt gataacataa ggacttgcct ccaccagtag gcataatcac
     1381 caaagtgtct tttctagcaa taatactttc tatgatttgt tgttgattgc ctttaaattt
     1441 ttcaaatcca aaatattctt ttaatgcttc cgatattgaa attgcttgta cgctcataaa
     1501 atagaataaa ataatcgttt acgttaaaaa cgttctcgca aacggagaac gtttcatagc
     1561 tttaatatac tcaaaagtac ggaaaactaa ggcctcataa gtgttaaaaa tctttatcta
     1621 attcttattt tatcgtattc atacccattt ataattatag atttgatata catcaacaat
     1681 attcttggtt gtgtctaatt tgtgtatctt tgcaacatat gaaaaccaat acttttgttt
     1741 gtattatggc gggtggcatt ggcagtcgat tttggcctgc cagcagaact gcaaaatcta
     1801 agcaattctt agatatttta ggaacaggaa gaaccctttt acagtcaaca tttgaccgtt
     1861 ttttaaagat ttgtcctaaa gaaaacatct ttattctaac gcatacagat tatattaatt
     1921 tggtaaaaga gcaattacct gatataactg atatacaaat tctgactgag ccagcccgta
     1981 aaaacacagc gccttgtatc gcttatgctg cctttaaaat caacaagatt aatcctgatg
     2041 ctaatattat tgtagcgcca tctgatcata ttattttaaa tgaagaggaa tttgtgcgaa
     2101 ttactgaatt agcgcttgac tttgtcgccc taaatgatgc actttgcaca ttaggtattc
     2161 gacctactcg accagacact ggttacggct atattcaata ttttgaagag gaaagtgcgg
     2221 aaggcataca taaagtaaaa acgtttaccg aaaagccgat taaagatgtg gctatccaat
     2281 ttgtgaaaag tggtgatttt ctttggaatg caggcatttt tatttggagt gcaaaaagta
     2341 ttttgactgc tttagaaaag aatttattag aagtttatga tgccttcgtt gaggcggaac
     2401 cacacatctt gacagacaaa gaaaaaactg ctgtggaaga ggcatttatt acctgtccta
     2461 gcatttcaat agactatggc attatggaaa aagctaaaaa tgtgtacact ttgccatcaa
     2521 gttttgggtg gagcgacctt ggtacatggg catctctata tgttgaaaaa gataaagact
     2581 atttccacaa tgctgttaat ggcaaaaatg tagttgttta cgactcaaca aataatatta
     2641 ttaatgttcc agaaaagaaa ttagtcgtca ttcaaggttt agaaaatttt attgtggtgg
     2701 atactgatga tgtcttattg atttgtaata aagacgaaga acaaagaata aaggaattta
     2761 cacacgatat aaaaatgaaa aaaggcgata aatatttgta agcacaagta tttactcttt
     2821 agaaaaaagt aagcggtctt atttttttta catatcttca atttcacatt ttcaaaaaat
     2881 taaaattcaa attgaattta tgtctaaatt atttgcaaaa aaatctattt ccaccttatt
     2941 acaagaagcg gaagactctg aaaaaggcct gaagcgaaca ctaaattctt gggcactcat
     3001 cgccttagga atcggcgcta ttatcggtgc aggcttattt gttagaacgg cggcagcagc
     3061 agcacaaaat gcaggcccgt ctgtaaccat cggttttata gtagcagcga taggctgcgc
     3121 attagctggc ttgtgctatg cagaactatc gtcatcgatt ccaatttcgg gcagtgcata
     3181 cacctatacg tatgctacta tgggcgaact attagcctgg gtcatcggct gggatttggt
     3241 attagaatat gccgttggtg cagccacagt tggcattgcc tggagtgaat atttgaataa
     3301 ttttctaaca caaatcttgc accttgcacc cattccatat cagtggtgtc attctccatt
     3361 tcagacatct gtagatggtg taagtggtat cgtcaatttg cctgcattag gcattgtttc
     3421 tatcattagc ttattactca taaaaggcat acaagaatct gcttttgtca ataatatcat
     3481 tgttattcta aaggtgagta ttgttgtgtt gattattgcc ttaggttggg gttttatcaa
     3541 tcctgaaaat catacaccat atattccagc tgctacacaa tatacagacc acgacggcat
     3601 tacttatcat tttggtggta tcatgggtat tttaggagct gctggaactg tgttttttgc
     3661 cttcattggt tttgatgctg taagtaccgc tgctcaagaa acaaaaaatc cgagcaagtc
     3721 tatgccgatc ggaattttag gttctttggc catttgcacc gtattgtata ttctgtttgc
     3781 acacgtatta acaggattgg aaagtgtcga tttctttaga gatccaaata aaggtggcga
     3841 agcatctgta tccgcagcta ttcaatcagc aatgcctggt tatagctggc tgagtaagtc
     3901 tgttaccatt gcaattctcg ccggattttc atcagtgatt ttagtattat tactaggaca
     3961 aagtcgtgtt ttctattcca tgagtaaaga tggcttattg cctaaagtat tttctgatat
     4021 tcatccaaca tacaaaacac catacaaagg caatttggcg atattggtat tagtagcagc
     4081 atttgcaggc tttgttcctg gcaatgtcgt tggacacatg actagcattg gtactttatt
     4141 tgcctttatg ttggtttgta ttgctgtcat cgttttacga aaaacagaac ccaatatgcc
     4201 aagacaattt aaaacacctt ttgtgccagt ggttcccata ttaggcgtta tcgcatgtgg
     4261 tgccatgata tttggtttgg gtgcagaaaa ttggatacga ctaatcgttt ggttgattat
     4321 aggcttcgtc atttattttg gctacagcaa aaaacacagc aaggtaaata atccaaaata
     4381 attagctcta aaaataaaaa caaaagggtt tgcgaatgca aacccttttg tttttaagtg
     4441 aaactactac tcgctaaaag gcaatgcaga tttaagtttg ttgtgtaatt tctcgttgta
     4501 actttctaac aagtaattaa aataattttt tgtggctttg ctcaagcagg aaatatatac
     4561 attcaatcgg tattgtattt catcttgtaa tgcatttatc aatggcaatg catctaaata
     4621 gtttgttgta tttgctcgaa tacgttcaaa atggttttct atggaaatat caatagattc
     4681 aagcgttttc ttgcctttgt tatacaaaga ataatagact ttttccatat caaagtcaaa
     4741 gtctgtggta ttaggaaaca aggcttttac ggtatcgggc aaatcatata acaagccttt
     4801 ttccatttgt atctctcgtt catacaaatg ttctaagaat ttttctggat tgacaaatac
     4861 atcacgccaa tcaatataat cttgccacaa gcctaagcga cgtgcattat tgcctaagtc
     4921 gattaccata aattcgcttt tgcttggcag cttacgactt ccacgtccaa tcatttgatg
     4981 atacaaagta agtgagcgcg tagcacgatt caacacaata cattcaaccg taggctcatc
     5041 aaatcctgtc gttaaaatcc caacagatgt tacaatggca tctggtgttt ctttcaacca
     5101 ttgcagcaca tcttttctat cttgtttgtt gctcgtgcta tctaagtggc gaataggaac
     5161 gccttggtct aagaaaaatt tttctaccga ctttgatgtg gctactgatg aattgaaaat
     5221 aagtgttttt ttattttttg ctttttcctg ataggcgcgc aatagctttt catgcatatc
     5281 aaaatgcatg tatattctgt ctagcgagct gactgtatag tcgccgtcga tacctacttt
     5341 caagccgcct aaatgaacat cataggtata tgtaattgca ttgcttaaat agcctttaga
     5401 aatcagctct tttatagaat cgccgatgat gagtcgctta taattttgag ctaaaggaag
     5461 attttggtta gagcttaatg gcgttgctgt aacacctaaa atcacggcat ttctaaagta
     5521 atgaaatatc tttctaaatg aattatagtg tgcttcatcg acaattacaa aatcgatatc
     5581 ttgcaaatat tcttcatctt ctttcaggcg attattcaaa gtttccacca tcgccaagaa
     5641 gcacaaaaac tcttcttgat ctggaatatc atctacttcg ctggtaatta atttacaatg
     5701 tatgcctgct tcttcaatgg catttgctgt ttgttttaat aactcaattc gatgtgttaa
     5761 tattaaaatc tttttctggt tttgttcaat atattttcgt gcaatttcag aaaaaatcac
     5821 tgttttgcca ccaccagttg gcaattgaaa aataatattt tcacggcgtt cattttcttc
     5881 caaatgttgg aaaatacgtt ttatgtattc ttcctgatat ggataaagtt gtttagctgt
     5941 ttcgggtctt gcttttattc ctgtaattct catgtacgta tagtcgttat aatgcaaaat
     6001 ctgtaccaag gctatttttc cattttttgc cactatatat atcttttcag ataaaactat
     6061 aaagacaaaa atcagttaca aatttgcgaa ctgcgcaata tacaaattta aatgcatgta
     6121 aaaaagaaga cgaattataa aatcattgcg ttgtatggta taattgtgga aaagcctttg
     6181 ttttggaaat aatgtgtaat catttcttgt ttttcatcac tacaaaccat aataaaatcg
     6241 ccgccccacg caccgagcga tttaattgtt cccggaaaat cttggaaaaa agtctcttta
     6301 actttctgca aattcaaggc atttgctata atattttcat gtacttctag ataatagcaa
     6361 aactcatcta gatcgatagc gagtattatt ttttgtgtaa gctcgttgat ctgttcaata
     6421 atggcagttt tatcaatatg gctattttta taaagttgta tgccttcgcg cgagttttgt
     6481 ttttttccta aatgaacaaa ataaatattt tctttaaatg ctggattgaa atccaaggca
     6541 gtaatttgtc gtgtattttg tgatgttaat tgatagctaa tcgcattttt tgcggtcgca
     6601 cacgcaatat cgtaagcact tccgccgaat gtttgttgta ataattcata cggattggca
     6661 tttgtccatt gtgcaattaa actaattaaa gtagaagagg accccaagcc ccaatgatta
     6721 ggaaaatcaa gttttgtagt tatgtgagcg ttgtggtgtg tgcaattaga tttttgcaag
     6781 agcacttgta atatctctgc tactgcaacg tttgtagtat gaagaattgt aaattgttca
     6841 tcaaattttg ctgagaacca tatttcattt ttatggtcga agctttccca agtaagtcca
     6901 ttttcactgc tatcttccac cttgagtgat tgcccaaatt tggtaggcaa tgcaaggccg
     6961 acagcaccat ctaaaatgaa atattcagcg ctgagtaata gcttgccgtt ggcatgaaag
     7021 gtttgtgtca tagaaaaaaa tcacagtaca actttagctt atttcacacc attttttgca
     7081 tttcgtaatt gctctaacat cacacgtgtt gtggtaaatg aaacaacttt gtctttaaaa
     7141 tattctaatg cataatcctt ttctgatgca gtagcatgat aataattcaa gatgttcagt
     7201 aagtgcattt tcatgtgtcc ttgttgaatg cctactgttg tcattgcttt taatgctgca
     7261 aaattttgtg ctaaaccagc aactgcacaa aatgccatta attgttctgc attaggattg
     7321 ccaagaattt ctaaactgcg ttttaccaat ggatgcaatt gcgtcaagcc gcctactgta
     7381 cctactgcta aaggcaagtc tatccaaaat ttaaaaatgc cattttctat agtgcaatgt
     7441 gttaatgaac cataagttcc attgcgtgca gcataagtat gaccgcaagc ctcgacagct
     7501 ctaaaatcat tgcctgtcgc aataatcacg gcatcaacac cattgtaaat gcctttgtta
     7561 tgcgttgttg ctctatgtgg atctacttct gccattctca cggctaaacg cattttttct
     7621 gcaaattctg ttgcggaaat agtgccaaaa ctacctaagt cttcaacctt acaagatact
     7681 gtagcacgtg cgatacaatt tggtgtgtaa ttagatacga tagctaaaat tatttttact
     7741 gttccgtatt ttggattatt gcctaccaac tctttaagcg tagatgcata ttcttccaag
     7801 caagtgttga tgaagttggc gcccatagaa tcgcaagttt cgaatgttgc tttaagctgg
     7861 aaatagccag cttcgtgttg cgtcatatct accaattcta tatccacaac tccgccacca
     7921 cgctgttcca tattgtagga aatatattta ctgccttcta atagttggtt ttttattgtc
     7981 gaaaaatcag cttttaattg tgcttcatcg cctttataga taaagtgtac ttgacctact
     8041 ttgcttgtgc ccaaaatttc tgtggtaaaa cctccgcgtt cactccaata ttttgcagca
     8101 ttactcatcg ctgctactac agagctttct tctatggcca ttggcaggca ataccattcg
     8161 ccattgatta aaaaattagg cgctactgaa aatggaaaaa aataattagt aatggtattt
     8221 tctgaaaact catctaatat tttttgtgta tgtaaatcgc gatgccaata agatatcaac
     8281 tctgattttg cggtttcgtc attgttcaaa taattttcaa ctaaccagtt tattttttgt
     8341 tctttgctaa gcttagaata gcctgatatc tttttcatat tatttatttc acttttaaaa
     8401 atgctttcgc cagttttagt ccttctattt ggaaatgtaa atactgttct aattcttcgt
     8461 aggaagatgt tgcatatttc aacaatgctg atgcttgtcc ataaacggca gtagcgttta
     8521 ttttttccat tagatagtaa gcatctaaaa agtttttgat gccaccggaa atgatatatt
     8581 gtttacacag tatcttatca ccaagttcat tttgtagttc attgacatac attgtcattt
     8641 ctgttgcgct atgtccaatt tttgttattg gctgataggc ttccattttc tgtttgtcgc
     8701 tacgtagcaa ctctatcata gaaaagtttg tacctccatg tgctgcaaac tcaagtgctt
     8761 cgattggcaa ttgtaataat gcgcgcaaac tttcttttcc aaacccttgt ccaacttctt
     8821 ttacaattac tttttgtgtt gttgtatcta agagttgttg tatcgtaata attggtgctt
     8881 gtgtaatctt gtctccttct ggttgtagcc attcttgcaa aggattgaca tgtataataa
     8941 tgccatcagc acggagtttg tctattaatt ggtcaatttg ataaagcttg ttattttgca
     9001 gtaatgcttc taattgtgct atgccaatat tgatataaaa aggaagtgta tcgccgataa
     9061 tatttctaaa gttaaaatct ttgagtcgtt catcactttc tagcaatgca cggcaagaac
     9121 ctaaacccat gcccatgccg aattgcttgc atgcgcgtgc caaatttgcg ttaatctgat
     9181 aggctttttc agtgccacct gtcatgctcg acacccaaat tggtgccgcc attgttttgc
     9241 ctaagaaacg tgttgatggt aatgcctcgt caggatgttt agataaaata ggttcgtaag
     9301 aaaagcgtgg atctaataat gcattttcta cttgtgattg aaatgccaaa tggatatggt
     9361 cttgttttcg tgttgccgct gttgggtctg ttgatatttt atttaaattt tccaagcata
     9421 acaaagttaa ggatattaat gtttgagagc attatttgtt tcgttattta tggttataaa
     9481 aacgagaatt ttgtattgtg tgcgttagcg attgtagcgg aaatactttt ttgtgtgatg
     9541 gcaattacta agcatagcaa aaaagattgt agcgaaaagc gcggcgcctg cttggctgtt
     9601 ggtgtggcgg cgcaacgccc ttatttagtc gattttgttt gtaaagaata ggctgctgtt
     9661 tgcaagtctt tgagccacaa atcgcgtttg gtgcgcaaga gccagcctgc tgtttgataa
     9721 atatgtccat ttggtgcttc gaagacgtcg atgacgaact gaaatctgtg gtcttgtttt
     9781 tctgatgata aaatgccaga aagttgatat tctttgactt tgaactgatt gacagttgta
     9841 aatagtgtgt ctttcgcttc tatgtttggt tctttaagat tgcgttttag ttgattgtat
     9901 atagagtcgc acattaattc gaagccttga gcgcctggtt ttgggccgtg gtcaactaag
     9961 ataaaataga cgtcgcggta ttcgtttttt gcttgcag
//
LOCUS       DAOQQV010000094         9994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2486_length_9994_cov_7.126673, whole genome shotgun sequence.
ACCESSION   DAOQQV010000094 DAOQQV010000000
VERSION     DAOQQV010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2486_length_9994_cov_7.126673"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLL59_09510"
     CDS             complement(<1..408)
                     /locus_tag="PLL59_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMY24086.1"
                     /translation="MSATVETKVYTSFDAASLVNTQRQFFNTGKTKDVEFRIQQLKKL
                     RQIIADNETAIVAALKADLNKSPMEAFATEFGFMLADIDHTLKDIRTLAKPRKVKTPL
                     FHQLGSSWIQAEPFGCTYIIAPWNYPFQLALSPV"
     gene            710..1207
                     /locus_tag="PLL59_09515"
     CDS             710..1207
                     /locus_tag="PLL59_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24087.1"
                     /translation="MKKQFLFALSAFFGISVLLLSSCTPDPVEYDETGYTGTYTGYHR
                     LVDSASLTPLLGDIDYSFLDTLVVTNGTSNTDGKIYAKSSYLGGNIIEIDISKTSSNI
                     TPKMIGTLNIESTVLTDAKIGSGSTATWNATKDIANVKINAGATYGVIVLPPSIRLWG
                     DFTKI"
     gene            1338..1856
                     /locus_tag="PLL59_09520"
     CDS             1338..1856
                     /locus_tag="PLL59_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24088.1"
                     /translation="MKKTILFTFVFAAFFAATFMTSCSDTSGPTCHSVAGSDTGAHAI
                     YIDALAAPLTPIRDSLHASVSGSDVTISSSALGRTITGEISASDCNSIVLDSIKFVAG
                     DTLVIPSAIGPGGFVKIYDIEAGGTGTITATGATTKIVIAKGKTNITSPINLTNLNGM
                     KLNLRGTFLKVN"
     gene            1992..4406
                     /locus_tag="PLL59_09525"
     CDS             1992..4406
                     /locus_tag="PLL59_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY24089.1"
                     /translation="MNKIVTLLLFISCIQMYAQQATIKGTLKDAKTNEILIGVNVTIE
                     NTTNGTTSDIDGHYELKVEPGKYNIVYSYVGYDNIVKTTHVKAGQIETIDVAMGEMQS
                     MLDELVVTSSKFERKIGEESVSIDVIKPAAMEKQNMNNVSDVLQRSSGVTIVDGQANI
                     RGGSGYSYGAGTRVLLLLDDLPILQADAGFPNWSGMPVENIGQIEVIKGAASALYGSS
                     AMNGIVNVRSAYPTSKPYAKFSVFGSTVDNPNKKDYIYDDASETLTKTSVDKKWWTLD
                     SITLKAGANGIFGRDTTIENTYNKRPYEAGFSFAYREKIGKLDIVAGGMFNKRQGHRW
                     GENETRGRVTLNLRYRINDKMSFGINTNLLSSKSQTFFLWGGDGINKYIPSAVTGIPT
                     ASRSFRTSVDPFFRYADTKGNSHKILGRYIRTAIDNSNNQDNSNDFYYGEYQYQRLID
                     KWNFTITTGAVANHVRSKSQLFGDTSHVGTNIAGYIQLDKKFWNRLTLTYGFRFESNR
                     LDKQKWETKPVSRVGVSVQAAKYTYIRTSFGQGYRFPTIAEKFITTELSPGFGIYPNR
                     DLTSETGLSVEVGIKQGIKLGKYVRAFADVAAFYTQYKNMMEFNFISNPTFGFQANNV
                     KSQTMIYGMEATIGGEGKLFGKFPTTLQLGYTYIVPKYKKYDENNDEYKDVAKYNVLK
                     YRIRHQFVGVWDIDFKGFTFGITGTYFSFMENIDAIFATILPSYYSYAASHLKKGSKL
                     SDIKPKFKGDFILDLRIGYHFTRENREFNFSFNVKNVANREYTLRPALVEAPRNYGFR
                     MDMTFN"
     gene            4536..6980
                     /locus_tag="PLL59_09530"
     CDS             4536..6980
                     /locus_tag="PLL59_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY24090.1"
                     /translation="MNIFKSIFLLIAILVLCSTTTLAQTITVKGKITDNKTKEPLIGV
                     NVTTKDNKASITNLNGDYEIQLSEGKQTLTISYVGYTEIQKDIDVKADAENILDITLE
                     EEAKNVLGDELVVTGSMFQKKASEEVISIEVVKPKLIQNTNVTRIDEALRRVSGINVA
                     DGQANIRGGSGWAYGVGSRVGFVLDGQTILSPDRGDIKWSLLPIETVGQIEVLKGASS
                     VLYGSSAMNGTIHLQTVVPTKKPQTRFTSFVSFYGPPKRKETKWWEIPQPSTGVSFMR
                     AHKVSNHFEYTVGGTAFIQNQQYQDGLEYLARLNYRTKWISKKNEKLSWGLAGSFSYN
                     RETEFFYWQNSRDGAYKPAANNIFTNIRITIDPYVTIFDKKNNKHEILTRTYFNRPSF
                     DTKTLLNNIDYRFTKQYVEKDLTIIAGANNQLLWINVPSFGGSPNKVANLFASYLQLD
                     KKYKRLTLSGGVRLETFTYQKKSGVTGSEFRKNGKLKFYLPGQWRAGLNYQATKKASI
                     RFNIGQAYRFPSFAERYVDETLGNADISRIDTTYQTVGDTVYVYTNTHTISRYTLAVL
                     PNVNLVPEYGWTTEFGYQQKLASKGKKYSGLIDIAFFWQQYKNLVDFNVNLSDTTVVA
                     IVKLKAENITDARIAGLDISLKHAITQKKHNLNIMTGYTYTLPIAIDKYLGYNLHKIG
                     PYFKAFFTHMFTPITGDTASYVLKYRNRHLITLDFEYVYDNKLTLGIDARYYSNQENF
                     DKLFLGIPGAGIDEYYRNMPKKGNFIVNARAFYTIKDKHTFGFIVKNLSNREYWLRVG
                     KLEPPINFTMQYRYEF"
     gene            7038..8237
                     /locus_tag="PLL59_09535"
     CDS             7038..8237
                     /locus_tag="PLL59_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HMY24091.1"
                     /translation="MKNSKYHYYNSLFKNQSLPLAYCDLDLLDKNIKDIANRTANAHK
                     TIRIASKSVRSTSILKRILDSHSIYKGLMCFSGSEAIFLSESGFDDLLLGYPIVNADE
                     ITKICEATKNAKTIVLMVDCIQHLEQINAIAKAIGTIQAVCIDIDMSTSLPGLHFGVM
                     RSPITSIQKAKKLVDAFEKFPNIKLSGVMGYEAQVAGLGENNPANGIKNYVIPFLKKY
                     SNSDYTYRRREVIAYIKSKNITLDFVNAGGTGSIESSKKEDWVTEITVGSGFYSPALF
                     DYYENFKHEPAVGFAVQIVRKPSQNIYTCLGGGYIASGSIGIDKQPKPYLPEGIKLSE
                     NEGTGEVQTPVEYNGKEQLSIGDTILFRHSKAGELCERFNQLQLVSAGKIVGTALTYR
                     GEGKCFL"
     gene            8314..9888
                     /locus_tag="PLL59_09540"
     CDS             8314..9888
                     /locus_tag="PLL59_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMY24092.1"
                     /translation="MTVLESTAIKITNPINGEIQYEIDEISAQQAKDIYEKASVVQTK
                     IQAMSVSQRVTEVLKIRDYVIAHSDFILDCIIAETGKCRTDAFAAEVFEVTDVIDVYT
                     KLAPKQLKDQKMNWPIFLMEKKSRQFREPLGTVLVITPWNFPFYQVIVPSLMSFLAGN
                     ATIVKPSEVTPLKPLWEHFFANSGFMKDAIQVVIGGKETGAHLLAQKPDKVHFTGSVN
                     TGRKLMEACAKQLIPIDLELGGKDPSIVFDDVDIDRTVNGIMWGAFTNAGQNCTGIER
                     LYVQEGIYDKLVPYLVERTKRLRTSHPNRDTKSAEDCDVGAVTAAFQVKIIEDHIEDA
                     IAKGATLLCGGIQEKGSHHIPPTIFENCTHDMKVASEETFGPTLAIMKFKTEEEAIRL
                     ANDSPYGLSASVWSKDTERGIRVARAIKCGNVCINNHMLNEANPYLPFGGVKDSGIGR
                     FKGEDGLLVFCNLKSVLLDKQGTLIEPQWYPYTNSKYKLLYAVMVSWFGKSRNWFKFI
                     ANVLPLDGIGNKEKFK"
BASE COUNT         3501 a         1660 c         1812 g         3021 t
ORIGIN      
        1 tactggcgac aatgccaatt gaaatggata attccaaggt gcaataatat aagtacagcc
       61 aaatggctct gcttgtatcc atgagctgcc caactggtga aataatggcg tttttacctt
      121 acgtggtttt gccaatgtgc gaatatcttt caacgtatgg tcgatatctg ccaacataaa
      181 accaaattca gtggcaaatg cttccatcgg cgatttgttc aagtcagctt ttaatgcagc
      241 tacaattgct gtttcattgt cagcaatgat ttgtcttagc ttttttaatt gttgaatcct
      301 aaattcgacg tcttttgttt tgcctgtgtt aaaaaactgg cgttgtgtgt tgactaagct
      361 tgctgcatca aatgatgtat aaacctttgt ttctactgta gctgacataa gtttcttttt
      421 gtgtgactaa tttaagaata gtaatcggta ttttaataga tatgttgttt tttttgcata
      481 tatcaataaa caaaaagcac ataattcata ttctgtttat ttattgaact tttttttcta
      541 aaatgattca tcgaaaaaat taaattcaat tcagcgcaag ctatttacta aaatattagt
      601 aacaatattt cgttaacttg gtttaatata acataagaaa aatagatttc cagctttttt
      661 gcgtattttg gctctatatt tgttatactt aatcaaacta acagtcttta tgaaaaaaca
      721 atttttattt gcacttagtg ctttctttgg aatttcagtt ttattattat caagttgcac
      781 accagatcca gtagaatatg atgaaactgg ctatactggc acttatactg gctatcatcg
      841 tttagtggat agcgctagct taacaccttt attaggcgat atcgattatt catttttgga
      901 tactttggtg gtgaccaatg gcactagtaa tacagatggt aaaatatatg ctaaaagttc
      961 ttacttaggt ggcaacatca ttgaaattga tatttcaaaa acaagctcaa acattacacc
     1021 aaaaatgatt ggtacactga atattgaatc aactgttttg acagatgcta aaatcggttc
     1081 aggcagcaca gcaacttgga atgcgacaaa agacatagct aatgtaaaaa taaatgctgg
     1141 tgctacttac ggtgttattg tattgccacc atctataaga ctttggggcg actttacaaa
     1201 aatttaattt tttcttgtta aaagtcaaaa agatacaaaa aagacacaaa aaaataacaa
     1261 aatatgaatt gtaattttgc aattcattta tttagtaata catttgaaac cattcattca
     1321 ctaaaaacaa cattaaaatg aagaaaacaa ttttatttac atttgtattt gcagcatttt
     1381 ttgcggcaac tttcatgaca agctgtagcg acacaagtgg tccaacatgc cattcagtag
     1441 ctggtagcga cactggtgct catgctattt acattgatgc tttagctgcg ccattaacac
     1501 caatccgcga ttcattacat gcttctgttt caggctctga tgtaaccatc tcttcaagtg
     1561 cattaggcag aacaattact ggcgaaatca gtgcttctga ttgtaactca atcgttttgg
     1621 attcaataaa atttgtagct ggtgatacat tagtgattcc ttctgcaatt ggacctggcg
     1681 gttttgtaaa aatctatgac atcgaagctg gtggcactgg tacaattaca gcaacaggcg
     1741 ctactactaa aattgttatc gctaaaggaa aaacaaacat tacttctcca attaacttaa
     1801 cgaacttaaa cggtatgaaa ttaaacctaa gaggtacttt cttaaaagta aactagtaga
     1861 aaatttatat cgaaataaaa aaactccaac ataattgttg gagttttttt tattgataat
     1921 tgacaataaa aaagataatt attttctatt acgttaattt aacataaatt agcagaaaat
     1981 aattaactaa catgaataaa attgtcacgc tactgctctt tatttcttgt attcaaatgt
     2041 atgctcaaca agcaacaatt aaaggaacat taaaagacgc aaaaaccaat gaaattttaa
     2101 ttggtgtgaa tgttaccatc gaaaacacta cgaatggtac tacatcagat atagatggcc
     2161 actacgaatt aaaagttgag ccaggaaaat acaatatcgt atatagctat gttggttacg
     2221 acaatattgt aaaaactaca cacgtaaaag caggacaaat tgaaacgatt gatgtcgcta
     2281 tgggcgagat gcaaagtatg ttagatgagc tggttgttac cagcagtaaa ttcgaaagaa
     2341 aaattggcga agagagtgtt tctatcgatg tgataaaacc tgcagctatg gaaaaacaaa
     2401 acatgaataa tgtaagcgat gttttacaac gcagctcagg tgttacaatt gttgacggac
     2461 aagcaaatat tcgtggaggc tctggctatt cttatggagc aggcacgcgc gtactactct
     2521 tattagacga tttacctatt ttgcaagcag atgctggatt tcctaactgg tctggtatgc
     2581 ctgtcgaaaa tataggacaa attgaagtga taaaaggagc tgcttctgca ttgtatggtt
     2641 catctgccat gaacggtatc gtaaacgtca gatcggctta tccaacctca aaaccgtatg
     2701 ctaaattttc tgtatttggt tctacagttg acaatccaaa caagaaagat tatatctatg
     2761 acgatgcatc tgaaacattg accaaaacaa gtgtagataa aaaatggtgg acattagaca
     2821 gcattacttt aaaagctgga gcaaatggca tttttggtcg cgatacaacc atagaaaaca
     2881 cgtacaacaa gagaccttat gaagctggtt tttcttttgc ttacagagaa aaaataggca
     2941 aattagatat tgtggctggt ggtatgttta ataaaagaca aggtcacaga tggggcgaaa
     3001 atgaaacacg tggtcgcgta actttaaact tgcgctacag aatcaacgat aaaatgagtt
     3061 ttggtataaa caccaattta ttatcatcaa aaagccagac atttttcttg tggggcggcg
     3121 atggtataaa caaatatatt ccgagtgctg ttactggtat tcctactgca tcacgcagtt
     3181 tccgtacaag tgttgaccct ttctttagat atgcagacac gaaaggaaat agtcataaaa
     3241 tattaggaag atatatacgc acagcaatcg acaatagcaa caaccaagac aactctaatg
     3301 atttttatta cggagagtat caatatcaac gccttattga taaatggaat ttcaccatca
     3361 ctactggtgc cgttgcaaac cacgttcgtt caaaatcgca attatttggc gacacttcgc
     3421 atgtaggtac aaacattgct ggttatattc aattagataa aaaattctgg aatcgtttaa
     3481 cgcttactta tggtttccgt ttcgagtcaa accgattaga caaacaaaaa tgggaaacaa
     3541 aaccagtatc gcgtgtaggt gtgagtgttc aagccgcaaa atatacctac atcagaactt
     3601 catttggaca aggctaccgc ttccctacta ttgctgagaa atttatcacc acagagttaa
     3661 gtccaggatt tggcatttat ccaaacagag atttaacttc tgaaactggt ttaagtgttg
     3721 aagttggtat aaagcaaggt ataaagttgg gaaaatatgt acgcgcattt gctgacgttg
     3781 cggcgtttta tacgcaatat aaaaacatga tggaatttaa cttcattagc aatcctacat
     3841 ttggcttcca agctaataat gtgaaaagcc aaactatgat atatggtatg gaagcaacaa
     3901 ttggtggtga aggtaaatta ttcggcaagt ttccgacaac cttgcaatta ggttatacct
     3961 atattgttcc taaatataaa aaatacgatg aaaacaatga tgaatataaa gacgttgcca
     4021 aatacaatgt tttaaaatac agaattcgtc atcaatttgt tggtgtttgg gatatcgatt
     4081 tcaaaggatt tacttttggt ataacaggca cctattttag ctttatggaa aacatcgatg
     4141 ctatctttgc aacgatatta ccaagctact attcttatgc tgcatcacat cttaaaaaag
     4201 gctcgaaact atctgatata aaaccgaagt ttaaaggcga tttcatattg gatttgcgta
     4261 ttggttatca ctttacaaga gaaaacagag aatttaattt ctctttcaat gtaaaaaatg
     4321 tagctaacag agaatataca ttgcgtccag cattagtaga agcaccacgc aactacggtt
     4381 ttcgtatgga catgacattt aactaacatt aaagacacat atttttaaaa acaaactttt
     4441 gtgccatcac tataatagtg tattgtgtac aaaagtttgt ttttttttat actatttaac
     4501 aaattgtaag gtatttttgc gcaaaataaa ttgaaatgaa tattttcaaa tctatctttt
     4561 tattaatcgc cattttagtc ttatgttcaa ctacaacatt ggcacaaaca attactgtaa
     4621 aaggaaaaat tacggataat aaaaccaaag aaccattaat aggtgtaaat gtaacaacca
     4681 aagacaataa agcatcgatt acaaacctca atggtgatta tgaaattcaa ttgtcagaag
     4741 gcaagcaaac actcactatt tcttatgttg gttatacaga aatacaaaaa gatattgatg
     4801 taaaagcaga tgctgaaaat atattggata ttacactaga agaagaagct aaaaatgtat
     4861 taggtgatga attagtggtc acaggttcca tgtttcagaa aaaagcaagt gaagaggtta
     4921 tttccattga agtcgtaaaa ccaaaattaa ttcaaaatac aaatgtcaca cgcatcgacg
     4981 aagcattgcg cagagtttct ggtatcaacg tcgctgacgg acaagctaat attcgcggtg
     5041 gcagtggctg ggcttatggc gtaggctcgc gcgtgggatt tgtgttagac ggtcaaacga
     5101 ttttatcacc agacagaggc gatatcaaat ggtctttact tccaatcgaa actgtaggtc
     5161 aaattgaagt attaaaaggt gcttcttctg tattatatgg ttcttctgca atgaatggta
     5221 ctattcattt acaaaccgtt gttcctacaa agaaaccaca aacgcgattc acttcttttg
     5281 tgtcatttta tggaccacca aaacgcaaag aaacaaaatg gtgggaaatt cctcagccaa
     5341 gtactggcgt atcgtttatg cgcgcacaca aagtgagcaa tcatttcgaa tatacagttg
     5401 gcggaacggc attcatccaa aatcaacaat accaagatgg tcttgaatat ttggcgcgac
     5461 tcaactatag aacaaagtgg attagcaaaa aaaatgagaa attaagttgg ggtttggctg
     5521 gtagcttttc ttacaataga gaaacagaat ttttctactg gcaaaactca cgtgatggcg
     5581 catacaaacc agcagcaaat aatattttca ccaatattcg tataacgata gatccttatg
     5641 taactatttt tgacaagaaa aataacaagc atgaaatatt aacaagaaca tatttcaata
     5701 gaccttcctt cgacacaaaa acattattaa acaatataga ctatcgtttt acaaaacaat
     5761 atgttgaaaa agacctaacc attattgctg gcgccaacaa tcaattgcta tggataaatg
     5821 tgccttcttt tggcggctct ccaaataaag tagctaattt atttgcttcc tatttacaat
     5881 tagacaaaaa atataaaaga ttgacattgt caggtggtgt ccgtttggaa acatttacct
     5941 atcaaaaaaa atctggtgtt acaggctctg aatttagaaa aaatggcaaa ttaaaattct
     6001 atctacctgg acaatggaga gctggattaa attaccaagc tactaaaaaa gcatcgatac
     6061 gatttaatat cggacaagcc tatcgtttcc caagttttgc agaaagatat gtggacgaaa
     6121 cgcttggaaa tgctgacatt tcaagaatag ataccaccta tcaaactgtt ggcgatacag
     6181 tatatgttta cacaaacaca catacaattt ctagatatac attagctgtt ttaccgaatg
     6241 taaatttagt gcctgaatat ggctggacta cagagtttgg ctatcagcaa aaattagcat
     6301 caaaaggaaa aaaatatagt ggcttaatcg atattgcttt cttctggcaa caatacaaaa
     6361 acttagttga tttcaatgtc aacctatctg acacaaccgt tgttgctatt gtaaaactta
     6421 aagcagaaaa tataacagat gcacgtattg ctggtttaga tatctcctta aaacatgcca
     6481 ttacacagaa aaaacacaac ttgaatatca tgactggtta tacttatacc ttgccaatcg
     6541 cgattgataa atatttagga tataacttac ataaaattgg accttatttt aaagcattct
     6601 tcacacacat gtttacacca atcacaggtg atacagcgag ctatgtttta aaatatagaa
     6661 atcgacattt aatcacttta gattttgaat atgtttatga caataaactt actttaggaa
     6721 tcgatgcacg ttactatagt aatcaagaaa attttgacaa attattttta ggaatacctg
     6781 gcgccggcat agatgaatac tatagaaata tgcctaaaaa aggaaacttc attgttaatg
     6841 ctcgtgcatt ctacaccatc aaggacaagc acacatttgg atttattgtc aagaatctca
     6901 gcaatcgaga gtattggctg cgagtcggca agttagagcc tccgattaac ttcaccatgc
     6961 agtatcggta cgaattttaa cttatttcta atatatatag atcctttcat gacgaaagga
     7021 tttattattt ttaagcaatg aaaaattcca aataccatta ctataattct ttatttaaaa
     7081 atcaatcact tccacttgcc tattgcgatt tggatttatt agacaaaaac ataaaagaca
     7141 ttgctaatag aacggcgaat gcccataaaa caatacgtat tgcatcaaag tcggtaagaa
     7201 gtacgagcat attaaaaaga atattagaca gtcattctat ttataaagga ttgatgtgtt
     7261 ttagcggaag cgaagctata tttttaagtg aaagtggttt cgatgattta ttattaggat
     7321 atccaatcgt caatgctgat gaaataacaa aaatttgcga agctacaaaa aatgctaaga
     7381 caatcgtttt aatggttgat tgcatacagc acttagaaca aatcaatgcg attgcaaaag
     7441 cgataggcac aatacaagcc gtgtgtattg acatcgatat gtcaacaagt ttgccaggat
     7501 tacattttgg tgtaatgcgc tcgccaatta ctagcataca aaaggcaaaa aaattagtcg
     7561 atgcatttga gaaatttcct aatataaaat tatcaggtgt aatgggttac gaagcgcagg
     7621 tggcaggctt aggtgaaaat aatccagcaa atggtataaa aaattacgtc attccttttt
     7681 taaaaaaata ttctaattca gattatactt atcgccgtcg tgaagtgatt gcctatatca
     7741 aatctaaaaa tataacttta gactttgtga atgctggcgg aacaggaagc atcgaaagta
     7801 gtaaaaaaga agattgggta acggaaatta cagttggttc tggcttttac tcaccagctt
     7861 tatttgatta ttacgaaaat ttcaaacatg aacctgctgt cggcttcgcc gtacaaatcg
     7921 tcagaaaacc gagtcaaaat atttatactt gccttggtgg tggatatatt gccagtggta
     7981 gcataggaat agacaagcaa ccaaaacctt atttgcctga aggcataaaa ctcagcgaaa
     8041 atgaaggtac aggcgaagtc caaacgccag tagaatataa tggcaaagag caattatcaa
     8101 tcggcgatac tatacttttc cgacatagca aagctggtga attgtgcgaa cgttttaatc
     8161 agcttcaact cgtatctgct ggaaaaattg taggcacagc attgacctat agaggcgaag
     8221 gaaaatgttt cctttaattt aatctaattt ctattgtaat tatttttcta tctaaccatt
     8281 tataggtata ttggagcatc aaatcaatat aatatgactg tgttagaatc tactgctatt
     8341 aaaattacaa atccgataaa cggcgaaatt caatacgaaa tcgatgaaat ttcagcacaa
     8401 caagccaaag acatttacga aaaagcaagt gttgtgcaga caaaaataca agcaatgtct
     8461 gttagccaac gcgtaacaga agttttaaaa attcgcgatt atgtaattgc acacagcgac
     8521 tttatattag attgtattat tgcagaaacc ggaaaatgca gaacagatgc atttgccgcc
     8581 gaagtgtttg aagttactga tgtcattgat gtctatacta aattagctcc taagcagctc
     8641 aaagatcaga agatgaattg gcctatattt ttgatggaaa aaaaatcacg tcaatttaga
     8701 gagcctttag gcactgtttt agtgattaca ccatggaatt tcccatttta tcaagtaata
     8761 gttccaagtt taatgtcatt tttagcaggc aatgctacta tagttaaacc atcagaagtt
     8821 acgccattaa agccactttg ggagcacttt ttcgcaaata gtggttttat gaaagatgcc
     8881 atacaagttg ttatcggtgg aaaagaaaca ggcgcacatt tattggcaca aaaaccagac
     8941 aaagtacatt ttactggaag tgtaaataca ggtcgtaagc taatggaagc ttgtgctaag
     9001 caattaattc caatcgactt agaattgggt ggcaaagatc caagtatcgt ttttgacgat
     9061 gtagatatcg acagaacggt aaatggcatt atgtggggcg catttacgaa tgcaggacaa
     9121 aactgtacag gcattgagcg tttgtatgta caagaaggaa tttacgataa attagtgccg
     9181 tatttagtag agcgtacaaa acgcttgcgc acatcacatc ctaatcgcga tacaaaatct
     9241 gctgaggact gcgacgtagg agcagtaacc gctgctttcc aagtaaaaat aatagaagat
     9301 catatcgaag atgccattgc taaaggagca acgttgttat gtggtggcat acaagagaaa
     9361 ggctcacatc acataccacc aacgatattt gaaaattgta cgcatgacat gaaagtagcg
     9421 tctgaagaaa cttttggacc tacattagcc atcatgaaat tcaaaactga agaagaagcg
     9481 attcgcttag ccaatgattc accgtatggt ttgagtgctt ctgtttggag taaagataca
     9541 gagcgtggca tacgagttgc gcgcgcaata aaatgtggta atgtttgtat caataatcac
     9601 atgctgaatg aagccaaccc ttacttgcca tttggtggcg taaaagacag tggaatcggc
     9661 cgttttaaag gcgaagatgg cttattggta ttctgtaatc taaaatctgt tttattagat
     9721 aaacaaggca cactaattga accgcaatgg tatccttaca ccaattcaaa atacaaatta
     9781 ttatatgctg tcatggtgag ttggtttggc aaaagcagaa actggtttaa atttattgct
     9841 aacgttttac cattagatgg catcggcaat aaagaaaaat ttaagtaaaa tattttgaca
     9901 cataaaacac caagtaattt tgtgcttggt gtttttttta ggtaatgtgg tcaaaaatgg
     9961 agcttttttg ggataaaaaa tggtattaaa ttta
//
LOCUS       DAOQQV010000095         9917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2520_length_9917_cov_8.083654, whole genome shotgun sequence.
ACCESSION   DAOQQV010000095 DAOQQV010000000
VERSION     DAOQQV010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2520_length_9917_cov_8.083654"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PLL59_09545"
     CDS             complement(<1..1087)
                     /locus_tag="PLL59_09545"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HMY24093.1"
                     /translation="MAEIDLQKIVSHCKEYGFIFPSSEIYDNLGAVYDYGPFGAELKN
                     HIKEYWWKSMVQMHENIVGIDAAIFMHPTTWKASGHVDSFSDPLIDNKDSKKRYRADV
                     LLEEKIDKLKEKGEKELAKKNLSDASDEEKAKASRDYAKGLTLEAEYIKAAEASDFKR
                     YKELIEEYEIACEVSGSKNWTDVRQFNLMFGTKLGAVDEDATELYLRPETAQGIFVNF
                     LNVQKTARMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFIKPGTQKEWFEK
                     WKQTRLQWHLALGTAQENLRFHVHEKLAHYADAAEDIQFKFPIGFKELEGIHSRTDFD
                     LGEHQKYSGKKLQYFDAELNQNYVPYVI"
     gene            1266..3602
                     /locus_tag="PLL59_09550"
     CDS             1266..3602
                     /locus_tag="PLL59_09550"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HMY24094.1"
                     /translation="MKKIFVLLSFFSVVLTLTAQNDFSYSTRYKNSLTAIYYTYAGTN
                     YTTNFRYGLYNEKTKKIILPMKYTRIWTSNEDDIFILEDSTKNWCMYSAKSNSFILKS
                     EYSEIKQFTDGLAIVAKKSSDGILQYGAADKTGKLIIPLQYKYLGNVSEDLICFSRDK
                     AYGYIDKNNKEIIAPMYRSAASFKSGLACVSLIDSTYYGYIDKKNNWIIKPKYLRGND
                     FKGNYAVVFTTKNYSLNSDNGGVIDKTGKEIIPLEYDNVTIEDDFFVVKKISKSSYII
                     ETKYGIFDFTGKVLLPIEYSAINKKFGTDFFEVQKDSKYSLIDKNAKMVLLGKYDYIY
                     TFTDFGISYLKKDNKFTIVDKTLRTIVPERGAFNVVFGKKNKVALLFKNVIEVYSETG
                     KLLKTIQQDNPNFYGTEFYSNDDSLKIKYNKCNYLYDLQTKKKTLLDYSEIFDFNEEG
                     IFPAKKSNYDFLDYTGKKLNTKSYYGVANFSDGIAAVQESLYSITYLVDKSFVKIKDI
                     NNVFEGPFSEGLAKAKAAYASNRYFYDKQGKEIISISSATETSDFKNGRASVKQQYNN
                     KFYFIDKSGKKINNELYDEVGPFSEYVAGVKLAGKTGYIDTTGKWIIPNKYDVGSPFY
                     KGVAIVKDGNEYYLINKKGKKINNDVYNGAKNPDNGTYPVQKGTNYGLIDSKGETLID
                     FKYQEVTPLHEGIVWAKKDGKWGLVNSSNKAITAFEYDNGDNCKNDYIKVSKDKKYGL
                     LDKQGKLVLPIIYDQMGSAYKGKILFVIDDGTQKLSLK"
     gene            complement(3676..5175)
                     /locus_tag="PLL59_09555"
     CDS             complement(3676..5175)
                     /locus_tag="PLL59_09555"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMY24095.1"
                     /translation="MANKENISILITGTGFSGVCVGIQLKKAGFHNFKILEKANDVGG
                     TWRDNTYPGAACDVKSNLYSYSFEPNPNWSRSYSAQPEILAYIKHCVKKYDLAQHIIF
                     DWEVKAAKYFEEDGIWELRNQRDEIQSANIAIIGNGPLHIPSLPDINGINDFKGAVFH
                     SAQWNHDFNLHDKNVCVIGTGASAIQFVPEIQAKVKNLYLMQRTAPWVLPKPDGDFST
                     TENVLFEKIPFIQKLYRDFIYYFNEAQVVGMMYEDKILKVGEMLGKYHINKHIKDKAL
                     RKKLTPNFKLGCKRVLLSNNYYQALAQKNVEVVTDGIEKFTKDSVVTKDGKSRKIDAI
                     ILGTGFHVADSFQYYNVKGKDGAELKDVLGASPEAYYGTSIHQFPNLFLMLGPNTGLG
                     HNSMVYMIESQTNYIIDAIQKMSEQGIKSIEVRKDVQTKFNEELQKKLEGTIWLSGCK
                     SWYLSEDGKNHTVWPGFTLEFRNRTKNINLSDYTIETKNVKKKKEALAQ"
     gene            5284..6474
                     /locus_tag="PLL59_09560"
     CDS             5284..6474
                     /locus_tag="PLL59_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMY24096.1"
                     /translation="MLSLRQLFLQHVAQTSDAPLALEIEKAEGVYLYDNNSKKYIDLI
                     AGIGVNALGHCHPKVVHAIQTQAAKYAHTLVYGEFIQSPQVQLAKYVTDLLPQNLSAV
                     YFTNSGAEATEGAMKLAKRFTCKTEIISCYNSYHGSTQGALSLNGDEYFKSNYRPLLP
                     DTRQIRYNNFDDIHLITKKTAAIFLEPVQAEAGIIVPDENYLHAVRKRCDETNTLLVF
                     DEIQTGCGRTGNLFRFQTENVVPDILLLGKAFGGGLPLAAFIASNEIMQVLQYNPVLG
                     HITTFGGNAVCCAAGLASLQEIEEQKLYAAAEEKAQLFIQLLQHDAIEKIHNCGLMMA
                     VYLDNFDNLRSVIQNCLANGLITDWFLFANNCLRIAPPLIITEDEIRQSCHIIIEALN
                     KVYK"
     gene            6471..6872
                     /locus_tag="PLL59_09565"
     CDS             6471..6872
                     /locus_tag="PLL59_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24097.1"
                     /translation="MKKLFFCCLILFLCSKNKLFAQQQKLPLPIFLQLIDSTKKIDIN
                     HATAFKHVELPKKFFNAFIGYIKDSSATLLPNVTSEVYYTFTSNNGKIINGDVYWNEQ
                     NSYIVFKVDGHVFVNAFSKEGVAQLKSIFKL"
     gene            6875..8335
                     /locus_tag="PLL59_09570"
     CDS             6875..8335
                     /locus_tag="PLL59_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMY24098.1"
                     /translation="MKKSFVFNVFFMVFVNMLIKPFWIFGIDRTVQNKLGEAEYGLFF
                     AIFNFTYLFQILLDFGFQNYSNREVAANTKRINDLLPNILVFKTILFLIYLVLCIAVA
                     YPLGYLSKSFIYIILANQILLSFNIYLRSNISAHQHFITDALLSVLDKLLMILFCLVM
                     LYGHFASFQLSIYNFALAQLAAYAITFITCLFFAFRLTEKLVFSFSFKHFKQIAKETM
                     PYAIIHFLMTTYYRIDGVMLERLQGAKGAVESGVYAQGYRIMEALNNIGYLFAGILLP
                     LFAYKLSKKIEIKTLLKSGLSIMFCVSVSIIVCLFWYRDDIMKLLYTSSNANYSAEVF
                     AYLLLNFFPVAMLYVIGTLLTANQNFKLMIYTLIFAVLLNVGLNFYLITQYGAKGAGI
                     ATLITQLFMLICYSIYCIRIFYIKVQFQYIVQLLAFVMLCIVLLFGLQKMQFSEQAII
                     NLLIHVGVYFMLMPLCCYITGLINIKTLQLKVGADD"
     gene            8528..9343
                     /locus_tag="PLL59_09575"
     CDS             8528..9343
                     /locus_tag="PLL59_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HMY24099.1"
                     /translation="MLEIFKIVLQWKKQVFIFTIVAIVASVIITMPAIMPPYFKSKQI
                     FYLSNPVSTDRAALFNEKEVGGVSIFGGKEDVNRFLSILISDEVSEKIIEKYKLIDHY
                     NLQGEDKALALYYTKREFYNQFKALRNDLGAIEVSILDTDAKLAATMVKDIVATSDSF
                     YRNMLTENKSTVLQLLDNQIATKKLNLQNNTNAIDEIQKLMAIRDQYAVSSSSAFKTI
                     YTVEAATPAVKKTKPVRWMIVVITALAAFLSASLFALLIELYKHADQYGFQRS"
     gene            9312..>9917
                     /locus_tag="PLL59_09580"
     CDS             9312..>9917
                     /locus_tag="PLL59_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24100.1"
                     /translation="MQTNTDFNVPSFFIRHDKPLFLAMASLSVLSFLLSIYLKIEILF
                     LLPFLVFFLGVCLLNFRFIFLLMLFVMPISVEMEIGSFGTDLPSEPIVILLSICTMLY
                     AIRNFHLVKNAIKHPIFIFLCFLYLWSWFTVIFSTNVFLSVKYMLAKSWYLLTFFVLP
                     IFLLDSTKRIRQFFWCLFIPSFISIIFIMIRHGMQGFTFDSI"
BASE COUNT         3245 a         1580 c         1689 g         3403 t
ORIGIN      
        1 cgatgacata aggcacataa ttctgattta attcagcatc gaaatattgc aatttttttc
       61 ctgaatattt ttgatgttca cctaaatcaa aatctgtacg cgaatgaatg ccctctaatt
      121 ctttaaagcc aattgggaat ttgaactgaa tatcttctgc cgcatctgca taatgtgcta
      181 atttttcatg aacatgaaaa cgcaaatttt cctgtgcggt gcctaaagct aaatgccatt
      241 gtaatcgtgt ttgtttccat ttttcaaacc actctttttg cgtgcctggc ttaataaaaa
      301 attgcatttc catttgctca aattcacgca tacggaaaat aaattggcgt gccacgattt
      361 catttctgaa tgcttttcca atctgagcaa taccaaaagg aattttcata cgtgctgttt
      421 tttgcacgtt caagaaattc acaaaaattc cttgagcggt ttccggtcgc aaatataatt
      481 cagtagcgtc ttcatctaca gcgccgagtt ttgtgccaaa catcaaatta aactgacgaa
      541 catctgtcca gttttttgaa ccagaaactt cgcaggcaat ttcatattct tcaatcagtt
      601 ctttatatct tttgaaatca gaagcttcag ctgcttttat atattctgct tctaaagtca
      661 atcctttcgc ataatcgcgc gaagcttttg ctttttcttc atcacttgca tcagataaat
      721 ttttcttagc taattctttc tcgccttttt ctttcagctt gtcaatcttt tcttctaaca
      781 aaacatccgc acgatatctt tttttagaat ctttattatc aatcaaagga tcggaaaaag
      841 aatctacgtg accgctggct ttccaggtag ttggatgcat aaaaatagca gcatcgatac
      901 caacgatatt ttcgtgcatt tgcaccatac ttttccacca gtactctttg atgtgatttt
      961 tcaattcagc accaaacgga ccgtagtcat acacagcacc taagttatcg taaatttcac
     1021 tcgacggaaa aatgaatccg tattccttac aatgtgatac aatcttctgt aaatctattt
     1081 cagccataat gcgcaaagat aattaatagt agtaagtaga agcaataagt aatgtgattt
     1141 ttttaatatt aaatcattcg tctgcgcctt tgatttctat gacaacaaca agaaatacct
     1201 aaaatcacgt attgtgtatt caaattttgg tatataattt ggagaaggac attaacaaga
     1261 agattatgaa gaaaatattt gtattgctaa gctttttttc agtggtttta acgctgacag
     1321 cacaaaacga tttctcgtat agcacgcgat ataaaaattc gctgacagca atatattaca
     1381 cgtatgcagg cacgaattat acgaccaatt ttaggtatgg cttgtataac gagaaaacca
     1441 agaaaattat tttgccaatg aaatacacca gaatttggac ttccaatgaa gatgatattt
     1501 tcattttaga agacagcaca aagaattggt gcatgtattc cgcaaaatcg aattcattta
     1561 ttttgaaaag tgaatacagc gaaatcaaac aatttacaga tggcttggca attgtagcta
     1621 aaaaaagttc tgatggaata ttgcaatatg gtgcagcaga taaaacaggg aaactaatca
     1681 ttccattgca gtataaatat ttaggaaatg tatcagaaga tttaatctgt ttcagtcgcg
     1741 ataaagcgta tggctatatc gataagaaca ataaagaaat tattgcacca atgtacagaa
     1801 gtgctgcctc tttcaaaagt ggattggctt gtgtttctct gatagattct acttattacg
     1861 gttatataga taaaaagaat aattggatta ttaaaccaaa atatttgcgt ggcaatgatt
     1921 ttaaaggaaa ttatgcagtt gtgtttacta cgaaaaatta ttctttaaat agtgataatg
     1981 gtggtgtaat tgataaaaca ggcaaagaaa ttattccatt agaatatgac aatgtaacga
     2041 tagaagatga tttttttgtc gtaaaaaaaa taagtaagtc atcttatatc atagaaacga
     2101 aatatggcat ttttgatttt acaggcaaag tacttttacc gattgaatat tcagctatta
     2161 ataaaaagtt tggtacagat ttttttgaag tgcaaaaaga tagtaaatat agcttaatag
     2221 ataaaaatgc aaaaatggta ttgttgggta agtacgatta tatttataca ttcaccgatt
     2281 ttggtatcag ttatcttaag aaagacaata aatttaccat tgtcgataaa accttaagaa
     2341 cgatagtgcc tgagcgtggt gcttttaatg ttgtcttcgg caaaaagaat aaagttgcct
     2401 tactttttaa aaatgtaata gaagtgtata gtgaaactgg aaaattatta aaaacgatac
     2461 agcaagacaa tccgaatttt tatggtacag aattttatag caatgatgat tccttaaaaa
     2521 taaaatacaa caaatgtaat tatttgtatg acctacaaac aaaaaagaaa acattgctag
     2581 attactcaga aatatttgat tttaatgagg aaggaatttt tccggcgaaa aaaagtaatt
     2641 acgatttctt ggattacaca ggaaaaaaac tcaacacaaa aagttattat ggtgtcgcca
     2701 atttttcaga tggcattgct gccgttcagg aaagtttata tagtataacc tatttagtgg
     2761 acaaatcgtt tgttaaaatt aaagatataa ataacgtttt cgaaggccct tttagtgaag
     2821 ggctggcaaa ggcaaaggct gcttatgcaa gcaatcgcta tttttacgat aaacagggca
     2881 aagaaattat ttctatttca tctgccacag agacaagtga ttttaaaaac ggaagagcat
     2941 ctgtaaagca acagtacaac aataaatttt attttataga taaatcagga aaaaaaataa
     3001 ataatgaatt atatgatgaa gtgggtccat tttctgagta tgtggctggt gtcaaattgg
     3061 ctggaaaaac tggctacatt gatactactg gaaaatggat aattccaaat aaatatgatg
     3121 taggctcgcc tttttataaa ggtgttgcta tagtaaaaga tggcaatgaa tattatttaa
     3181 taaacaagaa aggaaaaaaa ataaataacg atgtatataa tggtgcgaaa aatccagata
     3241 atggcacata tccagtgcaa aaaggaacta attatggatt gatagacagt aaaggggaaa
     3301 cgttgataga cttcaaatat caagaagtaa cgccactaca cgaaggcatc gtgtgggcaa
     3361 aaaaagatgg aaaatggggt ttggtcaata gtagcaataa agccataaca gcttttgaat
     3421 atgacaatgg cgacaattgt aaaaatgatt acattaaagt gtcgaaagac aaaaaatacg
     3481 gtttgctaga taaacaagga aagcttgttt tgccaataat ttatgatcaa atgggtagcg
     3541 cttacaaggg aaaaatttta tttgtaatcg atgatggcac acagaaatta tctttgaaat
     3601 aatatactaa actaacagct acttattgcg tgacagttgt cttttcgaat ttttatttta
     3661 ataaaattct gaaaattatt gtgccaatgc ttcctttttt ttctttacat tttttgtttc
     3721 gatagtataa tcactaaggt tgatattttt tgtgcgattt ctaaattcta aagtaaagcc
     3781 aggccaaacg gtgtgatttt tcccatcttc gctcaaatac cagcttttac aaccagagag
     3841 ccaaatggtt ccttctaatt ttttctgtaa ttcttcgttg aatttcgttt gcacatcttt
     3901 gcgtacttcg atagatttta tgccttgttc cgacattttt tgaatggcat caataatata
     3961 atttgtttgc gattctatca tatacaccat cgagttgtgg cctaagcctg tattgggtcc
     4021 tagcattaaa aataaattcg gaaactgatg tatgctggtg ccataatagg cttcaggaga
     4081 tgcgcctaat acatctttca attcagcacc atcttttcct tttacattat aatactgaaa
     4141 actatcggca acatgaaagc ctgttcctag aatgatagca tcaatttttc tcgattttcc
     4201 atctttggta acaacactat ctttcgtgaa tttttcaatt ccatctgtta ccacttccac
     4261 gttcttttgc gctaatgctt gataataatt attagacaac aaaacgcgtt tgcagcctaa
     4321 tttaaaattt ggcgttagtt tttttctaag tgctttatct ttaatgtgtt tgttgatatg
     4381 atatttacca agcatttcgc caactttcaa tatcttatct tcatacatca tgcccaccac
     4441 ttgtgcctca ttgaaataat aaataaagtc gcgatacaat ttttgtataa aaggaatttt
     4501 ctcgaacaac acattttctg tcgtcgaaaa atcgccatca ggttttggca aaacccaagg
     4561 tgcagtgcgc tgcatgagat ataaattttt cactttagct tgtatctctg gcacaaactg
     4621 tatggcactt gcgccagtac caatcacaca tacattttta tcgtgtaaat taaaatcgtg
     4681 attccattgt gcagaatgaa aaacagcacc tttaaaatcg ttaataccat tgatatcagg
     4741 caagcttgga atatgtaaag gaccattgcc aatgatggca atattggcgc tttgtatttc
     4801 atcgcgttga ttgcgaagtt cccaaatgcc atcttcttca aagtattttg ccgctttaac
     4861 ttcccaatca aaaatgatat gttgtgctaa atcgtatttc tttacgcagt gtttgatgta
     4921 tgctaaaatt tctggctgcg ccgagtaaga gcgcgaccaa tttggattag gctcaaatga
     4981 ataggaatat aagtttgatt tcacatcaca agctgcacca ggataggtat tgtcgcgcca
     5041 agtgccgcca acgtcattgg ctttctctag tatcttaaaa ttatgaaaac cagctttttt
     5101 taattgtatg ccaacacaca cacctgaaaa tcctgtgccc gtgatgagta tcgaaatatt
     5161 ttctttattt gccatagcat ttttttgtac tataaaggta ggctaactgg caatagcttg
     5221 tgtgtacaaa agctaatttt ttggctgcaa ataataagta aacattaatt aatatctttg
     5281 tgtatgcttt cactacgcca attattctta caacatgtag cacaaacttc tgatgcacca
     5341 ttggcattgg aaattgaaaa agcagaaggt gtttacttgt atgacaataa tagtaaaaaa
     5401 tatatcgact taattgcagg cattggcgtg aatgcgcttg gacactgcca tccaaaagtt
     5461 gtgcatgcta tacaaacaca agccgcaaag tatgcacata cgcttgtgta tggcgaattt
     5521 attcaatcgc cacaagtaca attggcaaaa tatgtaacag atttgttgcc acaaaatttg
     5581 tcagctgttt attttactaa ttcaggagca gaagctacgg aaggcgccat gaaattagca
     5641 aaacgattta cctgcaaaac agaaattatt tcgtgttaca attcttatca tggtagcaca
     5701 caaggcgctt taagcttgaa tggtgatgag tattttaagt cgaactatcg gccattattg
     5761 ccagatacaa gacaaatacg ctacaataat tttgatgaca ttcatttaat tacaaaaaaa
     5821 actgctgcca tatttttaga accagtgcaa gcagaagcag gcatcattgt gccagacgaa
     5881 aattacttac atgccgtgcg caaaagatgt gatgaaacaa acacactttt agtttttgat
     5941 gaaatacaaa caggctgtgg cagaacaggt aatttatttc gctttcaaac agaaaatgtg
     6001 gtgccagata ttttattgct tggaaaagca ttcggtggag gcttgccttt agcagcattt
     6061 atagccagta atgaaattat gcaagtgcta caatataatc ctgtgctcgg tcacattaca
     6121 acatttggtg gtaatgccgt ttgctgtgct gcaggattgg cttcgctgca agaaattgaa
     6181 gagcaaaaat tgtatgctgc cgctgaagaa aaagcacaat tatttattca attattacaa
     6241 catgacgcta ttgaaaaaat acataactgc ggcttgatga tggctgtgta cttagataat
     6301 ttcgataatc tacgaagtgt aatacaaaat tgtttggcta atggattgat tacagattgg
     6361 tttttatttg ccaataattg cttgcgtatt gcaccgcctt taattattac ggaagatgaa
     6421 attcgacaat cctgccatat tattattgag gctttaaata aagtatataa atgaaaaaat
     6481 tgtttttctg ttgtttgata ttgtttttgt gctcaaaaaa taaacttttt gcacaacaac
     6541 agaagttgcc tttgcctatt tttctacaat tgattgattc tacaaaaaaa atcgatatca
     6601 atcatgctac agctttcaaa cacgtagaat tacctaagaa atttttcaac gcatttattg
     6661 gctatattaa agattcatca gcaacacttc tgcctaatgt aacatctgaa gtttattata
     6721 cttttacaag taataacggc aaaataatca atggcgatgt atattggaat gaacaaaata
     6781 gttacattgt ttttaaagtg gacggccatg tttttgtaaa tgcattttcg aaagaaggtg
     6841 tggcacaatt aaaatcgata tttaaacttt aagcttgaaa aaatcctttg tgttcaatgt
     6901 gttttttatg gtgtttgtca atatgttgat aaaaccattc tggatttttg gtatcgatag
     6961 gacagtgcaa aataaattag gcgaagcgga atacggtttg tttttcgcta ttttcaattt
     7021 cacttattta tttcaaatat tattagactt tggttttcaa aattacagca acagagaagt
     7081 cgcagcaaat actaaacgta tcaatgattt gttgcccaat attcttgttt ttaaaactat
     7141 cttgtttttg atttatttag tgttgtgcat tgcagtagca tatccattag gatatctttc
     7201 aaaatctttt atttatatta ttttagctaa tcaaattcta ctctctttta atatttattt
     7261 gcgttctaat atttccgcac atcagcattt tattaccgat gctttattgt ctgtattaga
     7321 taaattattg atgatactat tttgtttagt gatgttgtat ggacattttg cttcatttca
     7381 attaagtata tacaacttcg cattagcaca attagcagca tacgcaatca catttattac
     7441 atgtttattt tttgctttcc gcctaacaga gaaattagta tttagctttt cgtttaaaca
     7501 ttttaagcag atagccaaag aaaccatgcc gtatgccatt attcattttt taatgacaac
     7561 gtattatcgc attgatggtg tgatgttgga gcgtttacaa ggcgcaaaag gtgcagtaga
     7621 atcaggtgtg tacgcgcaag gttacagaat tatggaagcg ctgaataata taggttattt
     7681 atttgctgga attttattgc cattgtttgc ctacaaactt tccaagaaaa tagaaatcaa
     7741 aacattgttg aaatcaggat tgtcaattat gttctgtgta agcgtgtcta tcattgtgtg
     7801 tttgttttgg tacagagatg acatcatgaa attattatat acatcatcaa acgcaaatta
     7861 ttctgcagaa gtatttgctt atttgctgtt gaattttttc ccagtagcga tgctatacgt
     7921 gatcggtact ttgcttacag ccaatcagaa ttttaagctg atgatttaca cattgatatt
     7981 tgcagtgttg ctgaatgtcg gtttaaattt ttatttaata acacaatatg gcgcaaaagg
     8041 tgctggcatt gctactttga ttacacaatt atttatgttg atttgttaca gcatttattg
     8101 cattcgtatc ttttatataa aagtgcaatt ccaatatata gtacaattgc tcgcttttgt
     8161 tatgctatgt attgtattgt tatttggact tcaaaaaatg caattttcag aacaggcaat
     8221 cattaattta ctgattcatg ttggtgttta ttttatgttg atgccgctct gttgttatat
     8281 aacaggactg ataaacataa aaacactgca attgaaagtt ggtgccgacg actaatttaa
     8341 ttttttcagg caaagatatg cgtatggctg atagaaatat ctcatattgc atattgatag
     8401 gaagatgatt gcatgatacg ctttattata atttacattt atcacatggt ctctgtaaat
     8461 attaatttca atatttacat acgctttttt atctttaagg tttaaattga taatcaataa
     8521 tttatttatg ttagaaatct ttaaaattgt attgcaatgg aaaaaacagg tttttatttt
     8581 taccattgtt gcaattgttg ccagtgttat cataacgatg ccagctatca tgcctccata
     8641 ttttaagtca aaacaaattt tttatctatc taatccagtg agcactgaca gagcggcttt
     8701 gtttaacgaa aaagaagttg gcggcgtgag tatatttggt ggcaaagaag atgtgaatcg
     8761 ttttttatct attttgatat ctgatgaggt tagtgaaaaa attattgaaa aatataaact
     8821 aatcgaccat tataatttgc aaggcgagga caaagcattg gccttgtatt atacaaaacg
     8881 agaattttat aatcagttca aagcattgcg aaacgactta ggtgctattg aagtgagcat
     8941 cttagataca gatgccaaat tagcggcaac aatggtgaaa gatattgttg caacatcaga
     9001 ctccttttac agaaacatgt taaccgaaaa taagtcaacg gtcttgcagc ttttagacaa
     9061 tcaaattgca acaaagaaat tgaatttgca aaacaataca aatgctattg atgaaataca
     9121 aaaactaatg gccatacgcg accaatatgc tgtttcctca tcgtcagcat ttaaaactat
     9181 ttatacagta gaagcagcga caccagcagt gaaaaaaaca aaacctgtac gctggatgat
     9241 tgtagtaata acagcacttg cagcgttttt gtcagcttca ttatttgcat tattgattga
     9301 actttataaa catgcagacc aatacggatt tcaacgttcc tagctttttt attcgccatg
     9361 ataagccttt gtttctcgca atggcaagct tgtcggtgtt gtcattttta ttaagtatct
     9421 atttaaaaat agaaatttta ttcttattgc cattccttgt atttttttta ggtgtatgct
     9481 tgcttaattt tagattcata ttcttattaa tgctttttgt tatgccaatt tctgtagaaa
     9541 tggaaattgg tagttttggt acagatttgc catcagagcc aatcgtaata ctcttgagta
     9601 tttgcaccat gttgtatgcg atacgcaatt ttcatcttgt aaaaaatgca atcaaacatc
     9661 cgatatttat ctttctatgc ttcttgtatt tatggagttg gttcactgtt attttttcta
     9721 caaatgtgtt tttgtctgtt aaatatatgc tggcaaaatc atggtattta ttaacttttt
     9781 ttgtcttgcc tattttttta cttgattcga caaaacgcat caggcaattt ttttggtgct
     9841 tgtttatacc aagttttatt agcattattt ttatcatgat tcgtcatgga atgcaaggct
     9901 tcacattcga ttccatc
//
LOCUS       DAOQQV010000096         9832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2554_length_9832_cov_7.820702, whole genome shotgun sequence.
ACCESSION   DAOQQV010000096 DAOQQV010000000
VERSION     DAOQQV010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2554_length_9832_cov_7.820702"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /gene="rnpB"
                     /locus_tag="PLL59_09585"
     ncRNA           complement(<1..256)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLL59_09585"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(295..1479)
                     /locus_tag="PLL59_09590"
     CDS             complement(295..1479)
                     /locus_tag="PLL59_09590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HMY24101.1"
                     /translation="MTQYPIIQLKKDKERSIQRFHPWVFSGALQIYDSNLKDGDIVEV
                     QDYKKNFLCIGHFYHGSIAVKILSFEWQTIDQKYWNNKIKQAFVLRKNLGLIENKNTN
                     CFRLIHAEGDNLPGLIIDIYDTTAVIQCHTIGMHLAINEIKNAITSTLDFIDTIYDKS
                     AAYLPKEYATTITNTYLLGTKKEIIAIENNNQFYIDVEEGQKTGFFLDQRDNRNLLAA
                     YCKDKSVLNTYCYSGGFSVYALNNKSKSVVSVDSSRNAINLTDANITLNFDDITTHQS
                     ICDDVFHYFKINENNFDVIILDPPAFAKSINKRHNAVQAYKRLNTEGFKRINKGGIMF
                     TFSCSQVVDKELFAATVMSAAIEAKRDVKILHYLSQPADHPINIFHPEGSYLKGLVLY
                     VN"
     gene            complement(1516..2007)
                     /locus_tag="PLL59_09595"
     CDS             complement(1516..2007)
                     /locus_tag="PLL59_09595"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HMY24102.1"
                     /translation="MKISIVVAAAENGVIGKDNQLLWKLSSDLKRFKSITNGHFILLG
                     RKTFESIGKPLPNRTSLIISRNFECDFENCFVFKTINDAIVFAAQQNQQEIFVVGGGE
                     IYKQILPLTNTIHLTIVHTEIVGDTYFEYDDSNWKVMHSAFIPKDEKNEFDSTYIILE
                     REN"
     gene            complement(2087..2968)
                     /gene="thyA"
                     /locus_tag="PLL59_09600"
     CDS             complement(2087..2968)
                     /gene="thyA"
                     /locus_tag="PLL59_09600"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HMY24103.1"
                     /translation="MQQYQQFLQHILENGIRKEDRTGTGTISVFGYQMRFNLNEGFPL
                     VTTKKLHVKSIIHELLWFIAGDTNIQYLCKNACNIWNDWPFAKYQKSSDYQGETMKEF
                     ATKIANNDAFAKQHGELGPVYGYQWRHWPDGNGGEIDQLTNLIHNIKNNPDSRRHIIS
                     AWNVADIEDMTLPPCHSLFQFYVIDGKLSCQLYQRSADAFLGVPFNIASYALFTMMIA
                     QVCNLKLGDFVHTFGDAHIYSNHVEQVQLQLSRAPLTLAQLKINPAINSIFDFRIEDF
                     ELINYQSHPHIKGEVAV"
     gene            3196..4881
                     /locus_tag="PLL59_09605"
     CDS             3196..4881
                     /locus_tag="PLL59_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HMY24104.1"
                     /translation="MFTKLLHLIVLILISNFLSLSNNNEIIPKPNIQVEKPGFFTISP
                     NTTIQIQDSACQNIVNYFQAYLHNFYQFNLIENTNQQNGIYLQIDTLLPLSSYSLVID
                     STTIVLKGGSESAVFYGIQTLIQLLPVTQNNPIQIACVSIYDAPKFEYRGMHLDVSRY
                     FFPVAFVKQFIDYLALHKINYFHWHLSDDQGWRIEIKKYPLLTEIGAWRNGTYAIARK
                     KIKKQATVIGNDGIRHGGFYTQSEIRDIVDYAAKRYITIIPEIEMPGHANAAIAAYPA
                     MSCFPSEPSKIKAIDLHKSSQRNKKMILQRLSLANDSIKKVVREYWGISDDVFCAGNE
                     NTFTFLEDVLSEVMSLFPSKWIHIGGDECPKSNWNRCPKCKARMETQGLKDAHELQSY
                     FIRRIEKFINGNGRTIIGWDEILEGGLADNAIVMSWRGEAGGIKAASANHKVIMAPNN
                     RVYFDHPRSLKNDKFKMFFGGYIPLRKVYNWPVIPKKLAKNKQQYILGGQACVWTEYV
                     PTIPVATSIIFPRIAALSESLWSHQDVKKYRDFKNRLKTQEQRYLLWQIKYKP"
     gene            4971..5765
                     /gene="bamD"
                     /locus_tag="PLL59_09610"
     CDS             4971..5765
                     /gene="bamD"
                     /locus_tag="PLL59_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HMY24105.1"
                     /translation="MFLKKPLYIVLIVTILASCSRQYDKVFKSNDMEAKLKLADQLYA
                     KKKYEKAIPIYEQLLTVLKGQKSVDEIYYKYANAHYLNGSYELAAFYFRSFYGTYPAS
                     EHAEQAAFDEAMCYLKQSPRFSLEQTNTQKAIEVFQEFVTRYPKSTKMEQANARIDEL
                     RAKLRKKSYESAYLYYKIGQYYAASVALSNFLKEYPEHDNPEKVDFLIVKALKKYADG
                     SYKEKQAERMAEEQKAYAAFKLKYPKSEYIADLEKLHQSKKNKNKN"
     gene            5769..6095
                     /locus_tag="PLL59_09615"
     CDS             5769..6095
                     /locus_tag="PLL59_09615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HMY24106.1"
                     /translation="MSTVKKEEGKVASAYIETRNLDELTAKTGNLYKSINVISARANQ
                     MNTKLKEELHRKLEEFASATDNLEEVFENREQIEISRYYEKLPKTSITALNEFLNDET
                     YFKDKE"
     gene            6132..7364
                     /gene="coaBC"
                     /locus_tag="PLL59_09620"
     CDS             6132..7364
                     /gene="coaBC"
                     /locus_tag="PLL59_09620"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMY24107.1"
                     /translation="MLKDKKILLGVCGSIAAYKAAFLVRLLVKEGAHVKVIMTGAATE
                     FITPLTMATLSKNECHTDYINATKTDWNNHVALALWADMLLIAPASANTLSKIANGNC
                     DNLLMAVYLSARCPVFIAPAMDEDMWKHASTKNNLQKIKTFGNTIISVNDGELASGLI
                     GEGRLAEPHEIVDFLQQQYKQNSANAALKIKNKVASKLFGKKILVTAGPTYEPLDPVR
                     FIGNHSSGKMGIALAESLHQQGAIVHLVLGPSTVAIHQDIKVTRVFSADEMYAVCKKD
                     FSSIDIAIFAAAVADYTPKQVAKQKIKKNDAEFSITLKKNVDIAYEFGKVKKSKQLAV
                     GFALETNNELAHAKGKLLKKNFDMVVLNSLNDKGAGFKHATNKITIVNRNNKLTKFEL
                     KNKEAVAVDIIQELEKLL"
     gene            7361..8290
                     /locus_tag="PLL59_09625"
     CDS             7361..8290
                     /locus_tag="PLL59_09625"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HMY24108.1"
                     /translation="MKKFLLAFITLCSLHIVAVAQELNCTVTINSPKNQNIDPQIFKN
                     METYIREFMNGRKWTNDTYKDVERIKCNLIINITEMPTEGEYKATAIIQSLRPIFNSN
                     ITTMLLNLQDKTFDFVYADMQNLDYNDNGYTSQLTSLLAYYAYIILAYDYESFAKDGG
                     TPYLLRAQQLCNNVPANEKSKYKGWNQFDGGVSFTTGTINRYVLVDNWLNPRYKDFRT
                     AFYAYHLNGLDRMYNDAAQGRGVITSSLTMLQKINADNPSLTPLRNFFFSKNSELINI
                     YSKCDMAERAVVITLLSTLDPTNGDKYKQIGKN"
     gene            8369..>9832
                     /locus_tag="PLL59_09630"
     CDS             8369..>9832
                     /locus_tag="PLL59_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HMY24109.1"
                     /translation="MLSSLSIKNYAIIESVEIQFDEQLNIITGETGAGKSILLGAMHL
                     ILGKRADTSVLRNNNEKCLVEACFKLQSEKLKDFFVVNDIEFDKETIIRREISANGKS
                     RAFINDSPVTLDVLKSLTEQLVDIHAQEETQNLLGKYFFCEILDELAKQSAIVKEYQI
                     SFQQYKHKQTRLRHLQEEQIRMQKEFDFISFQLNELVEANIDVDTFEGIENELGLLQN
                     AELIKRNLSDAYNLIDGNDFAALTQLLQANKQIGQLTSYHDVLLEMHDKLSVIIDDIK
                     SMTRQINNIAEATEYNEERVQELLDKQNTVNKLLQKHHVASVNDLLIIQSNLQEQTIA
                     YSNSSDEIIRLENEIKLEHQALLSTAQRVSKNRNEKIKTAESKVSSLLVDLGMPFGHI
                     ILEQKNADAEKLNAFGIDEIRLLFSANKGASPQALDEVGSGGEKSRLMLAIKSLVAET
                     ITLPTLIFDEIDTGISGEVAQKVGNILRELGTKHQVIA"
BASE COUNT         3402 a         1632 c         1708 g         3090 t
ORIGIN      
        1 atctatccat ctaccctcca acattaggcg agccacccta tattagcgtt ggtttacatg
       61 atgtttcagc gtttgaggtt tacctaaaga atgcattact gcaaactcat tttcacctta
      121 gatgtatttc tacaaatgtt attttctgtg gcactttctc tcgccataga cgagacagcc
      181 gttaactgtc aaactgctct atgctgttcg gactttcctt cccgatagct atcgggacga
      241 tagatcgacc tgcacgacaa atttaagcat aatcacttat gattccaatt tatttcaatt
      301 tacatataaa actaaacctt ttaaataaga tccttctgga tgaaaaatat tgataggatg
      361 gtcggcaggt tgtgataaat aatgcaggat ttttacatcg cgtttggctt ctattgcagc
      421 actcatcact gttgctgcaa acaactcttt atcgacgact tgagagcaag aaaaagtaaa
      481 cataattcca cctttgttta tgcgtttgaa cccttccgtg tttaatcgtt tatatgcttg
      541 cacggcattg tgccttttat ttatagattt ggcaaatgca ggtggatcga gtatgattac
      601 atcaaaatta ttttcattga ttttaaaata atggaataca tcatcacaaa tagattgatg
      661 cgtagtaatg tcatcaaaat ttagtgtgat gtttgcgtca gttaagttaa tcgcatttct
      721 agaactatca acagatacta cacttttgga cttattgttt aaagcataca cggaaaatcc
      781 accagagtaa caatatgtgt ttagcacact tttatcttta caatatgctg ccaataaatt
      841 tctattatcg cgctggtcga gaaaaaatcc agtcttttgt ccttcctcaa catcgatgta
      901 aaattgatta ttattctcta ttgcaataat ttctttttta gtgcctaaaa gatacgtatt
      961 cgttatagtt gtagcgtatt ctttaggtag ataggcagca cttttatcgt aaatggtatc
     1021 gataaaatcc aacgttgaag tgatggcatt tttgatttca ttaatagcaa gatgcatacc
     1081 aattgtatgg cattgtatta cggctgttgt gtcataaata tctataatta aacctggtaa
     1141 attgtcgcct tcagcatgta ttaatcgaaa gcaatttgtg tttttatttt caattaatcc
     1201 cagatttttg cgaagtacaa aagcttgttt tattttgtta ttccaatatt tttggtcaat
     1261 ggtttgccac tcaaagctta atatttttac agcaatgctt ccatgataaa agtgacctat
     1321 acatagaaaa tttttcttat agtcttgcac ttctacgata tcgccatctt ttaaattgct
     1381 gtcatatatt tgtaatgcac cagaaaaaac ccaaggatga aagcgttgaa tcgatcgctc
     1441 tttatctttt tttaattgta taattggata ttgagtcatt ttattttttg tccgaaattc
     1501 gtttattttc ttctgctaat tttctctttc taagatgata tatgtcgaat caaattcgtt
     1561 tttttcatct tttggtataa atgctgagtg cattactttc caattagaat catcatattc
     1621 aaaataggta tcaccaacaa tttccgtgtg tacaatagtg agatgaatag tgttcgtcaa
     1681 tggtaatatt tgcttgtaaa tttcaccacc accaacaacg aatatttcct gctgattttg
     1741 ttgtgccgca aaaacaatgg catcattaat tgttttaaaa acaaaacaat tttcgaaatc
     1801 acattcaaaa tttcttgaaa taattaagga agttctattt ggtaaaggct tgccaatcga
     1861 ttcaaaggtt tttcttccta atagaataaa atggccattt gtaatgcttt tgaatctttt
     1921 taaatcggaa gataatttcc aaagcaattg attgtcttta ccaattacgc cattttcggc
     1981 tgccgctaca acgatggaaa ttttcatttt atttttaatt gtttaatgat gcatgttgaa
     2041 tcgtgaaaat actatttatt tattatttac cattaaacat tcatcatcac acagctactt
     2101 ctcctttaat atgtggatga ctctgatagt tgattaattc aaagtcttct attctaaaat
     2161 cgaaaatgga atttatggct ggatttattt taagttgagc taatgtaagt ggcgcgcgtg
     2221 aaagttgtaa ttgtacttgc tctacgtgat ttgaataaat atgtgcatcg ccaaacgtat
     2281 gcacgaaatc tcctaatttt aaattgcata cttgcgcaat catcatcgta aacaaagcat
     2341 acgacgcaat attaaacggt acacctaaga atgcgtcagc actgcgctga tacaactgac
     2401 agctcaattt tccatcaatt acataaaatt gaaacaagga atgacaaggt ggcaaagtca
     2461 tatcttcaat atcggcgaca ttccatgctg aaataatatg acggcgcgaa tcaggattgt
     2521 tttttatatt gtgaatgaga tttgtaagct ggtcgatttc accaccattt ccgtcaggcc
     2581 aatgtcgcca ttgatagcca tacaccggac cgagttcacc atgttgcttg gcaaatgcat
     2641 cattgtttgc aatttttgta gcaaattctt tcatagtttc gccttgataa tcgctgcttt
     2701 tctgatattt tgcaaaaggc cagtcattcc aaatgttaca agcattttta cacaaatatt
     2761 gaatgttggt atcgcctgct ataaaccaca acaactcatg aataatagat ttgacatgta
     2821 gttttttagt agttactaat ggaaaacctt cattgagatt aaagcgcatt tgatagccaa
     2881 acacacttat tgtgcctgtg cccgtacggt cttccttgcg gattccattt tccaaaatat
     2941 gctgtaaaaa ttgttggtat tgttgcatgg cataaagata ctaaagtgac actattttag
     3001 caaaaacgat atgcacacgc tttgttcctt ggttagtagt ttaagtttta ctatgacata
     3061 agtccacttt tttcgatttt ttaaaattaa tattatttac atataaattg tatttattac
     3121 gtatgagatt tgtaaaattg tatatctatt tttgttattc tttgtacttt tgaaaatagg
     3181 aacttatatt gcttcatgtt tacaaaattg ctacatctta tcgttctgat tttaatatca
     3241 aatttcctta gtttatcaaa taataatgag attattccaa agccgaatat tcaagtagag
     3301 aaaccaggat tttttacgat ttctcccaat acaacgatac aaatacaaga tagtgcctgt
     3361 caaaatatag tgaattattt ccaagcgtat ttgcataatt tttatcaatt caatttaatt
     3421 gaaaatacaa atcaacagaa tggtatttat ttgcaaatcg atacattact gccactttca
     3481 tcgtattcat tagtaataga tagtactaca attgtgctaa aaggcggaag cgagtccgct
     3541 gttttttatg gcattcaaac cttaatacaa cttttacctg tcacacaaaa taatccaatt
     3601 caaattgctt gtgtttcaat ttatgatgca ccaaaatttg aatatagagg catgcattta
     3661 gatgtgagca gatacttttt tcctgttgca tttgttaagc aatttataga ttatcttgct
     3721 ttacataaaa tcaactattt tcattggcat ttaagcgatg accaaggttg gcgcattgaa
     3781 ataaaaaaat atcctttgct gacagaaatt ggcgcatggc gtaatggaac ctatgctatt
     3841 gctcgaaaaa aaataaaaaa acaagctaca gtaatcggca acgatggtat tcgtcatggt
     3901 ggtttttata cacaatctga aataagagat atagtagatt acgcggccaa acgttatatc
     3961 accattattc cagaaattga aatgcctggc catgcgaatg cggctatcgc ggcttatcct
     4021 gcaatgagtt gtttcccaag tgaaccttca aaaatcaaag caatagattt gcataaaagt
     4081 agccaacgga ataaaaaaat gattttgcaa cgcctttcgc ttgcaaatga ttccatcaaa
     4141 aaagtggtaa gagaatattg gggaatttct gatgatgttt tctgtgctgg aaatgaaaat
     4201 acttttactt ttttggaaga tgtcttaagt gaagtcatgt cattatttcc atcaaaatgg
     4261 atacatattg gcggcgatga atgtcctaaa tcaaattgga atagatgtcc aaaatgcaag
     4321 gcacgaatgg aaacgcaagg actgaaagat gcgcatgaat tacagagcta ttttattcgt
     4381 cgcatcgaaa aatttatcaa tggaaatggt aggacgatta ttggttggga tgaaatctta
     4441 gagggtggac ttgccgataa tgctatcgtc atgagttggc gaggtgaggc tggcggaatt
     4501 aaagcggcga gcgccaatca taaggtgatt atggcaccga ataatcgtgt atatttcgat
     4561 catcctcgaa gcttgaaaaa cgataaattc aagatgtttt ttggtggcta tattccactt
     4621 cgtaaagtat ataattggcc agttattccg aaaaaattag ctaaaaataa acaacaatat
     4681 attttgggtg gccaagcttg tgtgtggacg gaatatgttc caacaattcc tgtcgctacg
     4741 tcgattattt ttccacgaat tgccgcatta agcgaatcct tgtggtcgca tcaggatgta
     4801 aaaaaatatc gtgattttaa aaatcgccta aaaacacaag aacaacgcta tcttttgtgg
     4861 caaataaagt acaaaccata acattatttt aagctaaatt aacatcaaaa catcaataag
     4921 ctaattgatt tattagctta ttctctgatt aattcgtatc tttgcacctt atgtttttaa
     4981 agaaaccact ctatatagta ttaattgtca ccattttagc ttcttgcagt cgccaatacg
     5041 ataaagtatt taaaagtaat gacatggaag caaagctgaa attggctgac caactgtatg
     5101 ctaagaagaa gtatgaaaaa gcaattccga tatacgaaca attattgaca gttttaaaag
     5161 gtcaaaaaag tgttgacgaa atttattaca aatatgccaa tgcacattat ctaaatggca
     5221 gttacgaatt agcagcattt tactttagaa gtttttacgg cacttatcca gcaagcgaac
     5281 atgcagaaca agctgccttt gatgaagcga tgtgttatct taaacaatct cctcgctttt
     5341 ctctcgaaca aacaaacaca caaaaagcga tagaagtttt tcaggaattt gttaccagat
     5401 atccgaaaag cacgaaaatg gaacaggcaa acgcaagaat tgatgaatta agagcaaaat
     5461 tgcgtaaaaa atcgtacgaa agtgcttatc tttactacaa aattggacag tattatgctg
     5521 cttccgttgc tttgtctaac tttttgaaag aatatccaga acacgataat cctgaaaaag
     5581 tagatttcct tatcgtgaaa gcattaaaaa aatatgccga cggaagctat aaagaaaaac
     5641 aggcagaacg aatggcggaa gaacaaaaag catacgctgc ttttaaatta aaatatccaa
     5701 aaagcgaata catagcagat ttggaaaaat tacatcaatc taaaaaaaat aaaaataaaa
     5761 actagaatat gtcaactgta aaaaaggaag aagggaaagt agcatctgct tacatcgaga
     5821 ctagaaactt agatgaattg acggcaaaaa caggaaattt atacaagtct attaatgtaa
     5881 tttctgcacg tgccaaccag atgaacacaa aacttaaaga agaattacat cgcaagttgg
     5941 aagagtttgc ctccgcaaca gataatttag aagaagtatt cgaaaatcgt gagcaaatcg
     6001 aaatttctcg ctactacgaa aagttaccca aaacaagcat tacagcgttg aatgagtttt
     6061 tgaacgatga aacatacttc aaagacaaag aataaaattc gcatttgctg actcaataaa
     6121 agtttatcac gatgctgaaa gataaaaaaa tattactcgg tgtttgtgga agtatagctg
     6181 cctacaaggc agctttttta gtgcgcctac tggtaaaaga aggtgcacat gtaaaagtca
     6241 tcatgactgg tgctgccact gaatttatta cacctttgac catggcaacg ctttccaaaa
     6301 atgagtgcca tacagattat atcaatgcta ctaaaacaga ttggaataac catgtagcat
     6361 tagcactttg ggcagacatg ctgctgatag cacctgccag tgccaacaca ctttctaaaa
     6421 ttgccaatgg taattgcgat aatttgctga tggccgtgta tttgtcggca cgctgccctg
     6481 tgtttatagc accagccatg gacgaagata tgtggaaaca tgcatcgact aaaaataatc
     6541 ttcaaaaaat taaaactttc ggcaatacaa ttattagcgt aaatgatggt gaattagcca
     6601 gtggtttaat aggagaaggc agactggctg aaccacacga aatagtagat tttttacaac
     6661 aacagtataa acaaaatagt gcaaatgctg cattgaaaat taaaaataaa gtagcatcaa
     6721 aactattcgg aaaaaaaatt ttagtaactg caggacctac gtatgagcca ttagatccag
     6781 tgcgttttat tggcaatcat tcttctggaa aaatgggcat tgctttggca gaaagcttgc
     6841 atcaacaagg tgcaatcgta catttggtat tagggccatc tacagtagca atccatcaag
     6901 acatcaaagt aacgcgtgtg ttttcagcag atgaaatgta tgctgtatgt aaaaaagatt
     6961 tttcgtcaat tgacattgct atttttgcag cagcagtagc cgattacaca ccaaaacaag
     7021 ttgctaaaca aaaaatcaaa aaaaacgatg ctgagttttc tattacatta aagaaaaatg
     7081 tggacattgc ttacgaattt ggtaaagtaa aaaaatcgaa acaattagca gttggttttg
     7141 cattggaaac gaataatgaa ttagcacatg caaaaggaaa attacttaaa aagaattttg
     7201 atatggtagt gctcaattcc ttaaatgata aaggtgctgg ttttaaacat gcgacaaata
     7261 aaattacgat tgtaaatcgt aacaataaat tgactaaatt cgagttaaag aataaagaag
     7321 ctgtagcagt tgatattatt caagaattgg aaaaattatt atgaagaaat ttcttttagc
     7381 ttttattaca ctttgctcac tgcatatagt tgctgttgca caagaattga attgtacggt
     7441 aacaatcaac tcaccaaaaa atcaaaatat cgaccctcaa atattcaaga atatggaaac
     7501 ctatattcgt gaatttatga atggtagaaa atggacaaac gatacgtata aagatgtgga
     7561 gcgcattaaa tgcaacctca tcatcaatat tacagaaatg ccaacagaag gtgagtataa
     7621 ggctaccgca attatacaat cattacgacc tatttttaat agcaacatca ctacgatgct
     7681 attgaatcta caagataaaa catttgattt tgtatatgct gatatgcaaa atttagacta
     7741 caacgataat ggttatacat ctcagttgac atcattgctg gcatactatg cttatattat
     7801 attggcatac gattacgagt catttgctaa agatggtggc actccttatc ttttgcgtgc
     7861 acagcagtta tgtaataatg taccagcaaa tgaaaaatct aaatataaag gctggaatca
     7921 attcgatggc ggcgttagct ttacgacagg aactatcaat agatatgtgt tggtagataa
     7981 ttggctcaat ccacggtata aagattttag aacagcattt tatgcatatc atctcaacgg
     8041 attagataga atgtataatg acgcagcaca aggacgtggc gtgattactt cttcgctgac
     8101 tatgttgcaa aaaataaatg cggataatcc aagtttgaca ccattgcgta atttcttctt
     8161 ctctaaaaat tcggagctta tcaatattta ctcaaaatgt gatatggcag aacgcgctgt
     8221 ggtaataaca cttttatcga ctttagatcc aactaatggc gataaataca agcagattgg
     8281 aaagaactag tgtgatattc aaatagaatt ttgtttaatt catcacttca gtttctaaat
     8341 ttcaaacgct aattcgtatt tttatagcgt gttaagctca ctttctataa aaaattatgc
     8401 catcattgag tcggttgaaa tccaattcga tgaacaactc aatattatta ctggcgaaac
     8461 tggcgcagga aaatctattt tgttaggtgc catgcatctt attcttggta aacgcgccga
     8521 tacgagtgtg ttgcgaaata ataatgaaaa atgtttggta gaagcgtgct tcaaattaca
     8581 atcagaaaaa ctgaaagatt tttttgttgt taatgacatt gaatttgaca aagaaactat
     8641 tattcgcagg gaaataagtg ccaatggcaa atctcgagca tttatcaatg attcgcctgt
     8701 aacattagat gtattaaaat cattgacaga gcaattagta gatattcacg cacaagagga
     8761 aacgcaaaac ttattaggca agtatttctt ttgtgaaata ttagacgaat tggcaaagca
     8821 atcggcaatt gtaaaagaat atcaaatctc ttttcagcag tacaaacaca agcaaacacg
     8881 cttgcgacat ttgcaagaag aacaaattcg catgcaaaaa gaatttgatt tcatctcttt
     8941 tcaattgaat gaattagtag aagcaaatat tgatgttgat acatttgaag gcattgaaaa
     9001 tgagcttgga ttactgcaaa atgccgaact tatcaaacgc aatttgtctg atgcctacaa
     9061 tctcatcgat ggcaatgact tcgctgcatt gacacaatta ttacaagcaa ataaacaaat
     9121 aggacagttg acttcttatc atgatgtgtt attagaaatg catgataaac tatcagtaat
     9181 tattgatgat ataaaaagca tgactcgtca aatcaataac attgcggaag caacagaata
     9241 caacgaagaa cgtgtgcaag aattattaga caaacaaaat acagtcaata aattattaca
     9301 aaaacatcat gttgcaagcg taaatgattt attaattatt caatccaatt tacaagagca
     9361 aacaatagcc tattcaaatt cgtcagatga aattattcgc ttagaaaatg aaattaaatt
     9421 agaacaccaa gcgctcttgt caaccgcaca acgcgtttct aaaaatagaa atgagaaaat
     9481 aaaaacagca gaaagtaaag tttcttcatt gttagttgac ttaggaatgc cattcggtca
     9541 tattatttta gaacaaaaaa atgctgatgc ggaaaaactg aacgcttttg gaattgatga
     9601 aatcagatta ttattttcag caaataaagg agcttcgcca caagcattag atgaagtagg
     9661 tagcggtggc gaaaaatcga ggttgatgtt ggctataaaa agtttagtag ctgaaacaat
     9721 aaccttacct actttgattt ttgacgaaat agataccgga atttcaggtg aggtagcaca
     9781 gaaagttggc aacattctcc gagagttagg cactaaacat caggtgattg cc
//
LOCUS       DAOQQV010000097         9769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2580_length_9769_cov_8.112312, whole genome shotgun sequence.
ACCESSION   DAOQQV010000097 DAOQQV010000000
VERSION     DAOQQV010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2580_length_9769_cov_8.112312"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..331)
                     /locus_tag="PLL59_09635"
     CDS             complement(113..331)
                     /locus_tag="PLL59_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24110.1"
                     /translation="MKKNRLFLALLSIIAISVSSCNKTYNCECSERGRVLDVIPITSL
                     GKMGAKDVCDTYQNENNRMGAHQVCVIK"
     gene            452..1174
                     /gene="recO"
                     /locus_tag="PLL59_09640"
     CDS             452..1174
                     /gene="recO"
                     /locus_tag="PLL59_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HMY24111.1"
                     /translation="MLDKTEGIVLRQVRYAESSMIISIFTQKYGLLSFMVQGVRSNKS
                     KQKGNMLQVLNILMLDIYLKEQRNLNRIKEHSAAYIYKNIPNDFAKQSIGIFCIELIS
                     KCIKEQEVNERLYQYLVAFLIDLDNTKERIENKPLFFLLEMASILGFEPSLQNILHGI
                     YFNLESGRFEDNNSHTQNCLSAHETELLKKLIAMYYEKNDWQFNSMERKILLDKLLLY
                     FRWHIAEFTTLKSPAVLSEILR"
     gene            complement(1186..2256)
                     /gene="prfA"
                     /locus_tag="PLL59_09645"
     CDS             complement(1186..2256)
                     /gene="prfA"
                     /locus_tag="PLL59_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HMY24112.1"
                     /translation="MLDKLELIYFRYKDLESQLSDPEVVSDMQKFKKTSKEYKDLEPI
                     VNAYLTYKNLLSNIDFNKQILSEEKDAEMRELAKAELDEQQAQLPNLEEEIRYLLIPK
                     DPEDEKNAILEIRAGTGGDEASIFAGDLLRMYLKYCENKGWTTEIMNENAGTAGGYKE
                     VVVEVSGDGVYGVLKYESGVHRVQRVPETESQGRVHTSAASVAVLPEAEEVDVEINMA
                     DIKKDVYRASGAGGQHVNKTESAVRLTHLPTGIIAECQDGRSQHKNYESALKVLRTRL
                     YEKALNEHNEAIAKQRKTLVSTGDRSAKIRTYNYPQGRVTDHRINLTLYNLSEIVNGD
                     LQDIIDKLTFEENAEKLKVGGL"
     gene            2362..2778
                     /locus_tag="PLL59_09650"
     CDS             2362..2778
                     /locus_tag="PLL59_09650"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HMY24113.1"
                     /translation="MDSQDTGSLTAIIAALGIFFLFFFLLSIFFIVCHWKIFTKAGRK
                     GWECLIPFYNLYVQLQITKQPTIWLLFFLIPFVNIYFGIKHVHGLSLAFGKDVGFTIG
                     LLFVPFVFIPILAFGSAHYVYNQDENLLFNEIQEIK"
     gene            2922..4895
                     /locus_tag="PLL59_09655"
     CDS             2922..4895
                     /locus_tag="PLL59_09655"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24114.1"
                     /translation="MLASDVTNIQYFVQIVKEIRPHKNNQSEVANQHIQTFIHRLENN
                     ATALHEFRSQCQILIQQSSLQEIFSDNNDVFGSVNIFEQLAKDIKYRFLAPLIDSSSL
                     NYKLSIIFSKKTDYIWINSICDEHWIRLFQLSGIDNQFAEAFIFKKIQNAAMMLSYKI
                     AALGLDNEFLNRFAMEDELISPFIEQNKELVFYIDNFTTHTSELASLSNTNKQIVVLL
                     QQCMESIQKIRKNTTSYGTSLRQTYLLNKTAVNIEHLKILLDIYDVADFNLTRLVCFF
                     KNAVYNENNRYSIRHTLSKNIGHLAYQIAEHKGTVGEHYITSKKEEFVHHFKAAFGGG
                     FIITLMVFLKVGASTFPITDFWKAILYSIIYASGFVLIHLLGFTVATKQPAMTASALA
                     KELDKNKNGINNLQNIALLMAQVSRSQIIAIFGNLASVFPVTLFITWLLHSCFHFAVS
                     DADHAMHYLKEIHPLYALSAWYAAIAGFYLFVSGIITGYFDNTVIYSRIPERIRQKTT
                     WKKFLGEKRLAQLANYLQHNLGSIMGNIMIGFFLGTATFIGTTLGLPFDIRHITISTG
                     LFAIAIETLHFQIALNMLFGVLLGLFGIALFNVISSFGFAFYVALKSRNIDSSELAQL
                     PLILWQYFIKHPSDFIYPPKTERTVENVFGKAD"
     gene            complement(4892..5704)
                     /locus_tag="PLL59_09660"
     CDS             complement(4892..5704)
                     /locus_tag="PLL59_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMY24115.1"
                     /translation="MRNFRDKIAVITGAGSGIGQALAIELAKKGAILVLSDKNETGLA
                     DTKKRVETIGAQCHTYVVDVSDVKAVTAFCTKVLKEHEHIDLLFNNAGFALGKISFRD
                     VKMEDFNRLMDVNVYAVVLHTKLFLETLIARPEAVIVNISSLFGLIGMPDQVPYCTTK
                     FAVRGFTESLRAEMYDTNVQVHSVHPGGINTNIYKNAVHYKHDANEERIFQKMLERTS
                     PESAAKTIIRGVERNNARILIGADAKLLDNIARTLPEGYTTIIQKGKKAMGF"
     gene            5857..6462
                     /locus_tag="PLL59_09665"
     CDS             5857..6462
                     /locus_tag="PLL59_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMY24116.1"
                     /translation="MNLRFFSIASILLFSAFTLFAKNDVAVKETTINAQKSSIEWIGK
                     KVLSQHSGKLNIKSGALSTKNGILGGGTFTIDMTSITCDDIKDADYNKKLVGHLNSTD
                     FFNVAEHPTAIVKITKVLKINNKANAYNLTADLTIKGITNSITFPATFEKNGNVFEGN
                     ANITIDRTLWDIKYKSANFFEGLGDKAIKNEIELKVNIVSN"
     gene            complement(6590..8323)
                     /locus_tag="PLL59_09670"
     CDS             complement(6590..8323)
                     /locus_tag="PLL59_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMY24117.1"
                     /translation="MLIPFLNLIFNADKMVLQHVEASSFGITSLKEFIGTSFNNKVIQ
                     YIQSADTIEAGKSQALLFICLFTVFVFLVKNVFRYSAVHFLASMRTGIASTIRQELYD
                     KILHLQVLYFSDKRKGDVLTRLTNDVQEVEYGILHFVEVAFKEPVTILATLLTMLILS
                     PKLTLFVLIILPLSGYFIGKIGKTLKRASSTAQHLQGVLNSIVDETISGIRVIKSFTA
                     EQSFSNKFRNINEQHNRVGTSMIRKRDLSTPLSEFLGISVVVVVLYVGGKMIIHHTSP
                     LSAEAFIAFIVIFSQIIQPAKAFSNAFYFIQKGMASLRRIEEVLQEENKVNDIAQALP
                     LLDFKDKIEFKNVCFAYHERTVLKNISITIPKGKKVAIVGVSGAGKSTFIQLLLRFFD
                     IDSGEILIDGVNIKNYKQQALRSQMALVTQHTTLFNDTVSNNIAFGLLVNEPQIVHAA
                     KQAHADIFIQKMPQQYDSLIGDNGTKISGGEQQRLSIARAIYKDAPILILDEATSHLD
                     SNSEKLVQDALQNLWKNKTAIIIAHRLSTVQLADEILVLKEGELIESGTHQQLLQLQG
                     EYKKLVDLQQL"
     gene            8465..8863
                     /gene="rbfA"
                     /locus_tag="PLL59_09675"
     CDS             8465..8863
                     /gene="rbfA"
                     /locus_tag="PLL59_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HMY24118.1"
                     /translation="MESIKQRQVAKTIQIAMSEVVQKDLGGILGGAMLTISGVRVTPD
                     LYTARIYVSIYNHAKPDTVLDNLDKNNKMIRGLLGNKIKNKVRSIPQLEFFKDDTLED
                     VQNLERIFSEIKKKDAEIEKMRGKNDENEQ"
     gene            8883..>9769
                     /locus_tag="PLL59_09680"
     CDS             8883..>9769
                     /locus_tag="PLL59_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24119.1"
                     /translation="MNLELNIAKRYLFSKKSTNSINIISLVSGAAMLFGTMALILVLS
                     VFNGLEDLVKSLYAVFYPDIAITVKTGKTFEITNELRQQLNTIDYIDAFSFTLEENAL
                     LEYAEQQHICTVKGVDKNYFKVINKFDTFIIAGKKILQKDSINFGIVGVGVAQKLGLD
                     PTNAFLPLTLYMPKKTASSFSSMENAFNKSYVTPSAAFAVSDEFDTKYTFVPLDFFQQ
                     LTDNFGRASQIDIRLKNQTQSALAIAAIQKKIGNNFILKSRYEQNEVLYKILKSEKWI
                     TFSILLLIVIIASFNIIGA"
BASE COUNT         3172 a         1730 c         1632 g         3235 t
ORIGIN      
        1 actttatcgc tggtaggttg ctctccagca gagcctactt cctgttcaaa tggattgcta
       61 aaattaatac cattttgcac ccaaaaaaag caccattttt gaccacatta ccttatttga
      121 tgacgcacac ttggtgagca cccattctat tgttctcatt ttgataagta tcacaaacat
      181 cttttgcgcc cattttacct aaagatgtga ttggaataac gtcaaggaca cgaccgcgct
      241 cactacactc acaattgtag gttttattgc aagaagatac acttatcgct ataatcgaaa
      301 gtagagctaa gaataatcga ttttttttca tgtttctgta ttattagttt aaagatacaa
      361 atctttgata aaaatgaaaa aaaatagttt agctattttg aaacaagcca aaaaaaatga
      421 actatccttg taaaaaaagt atttttacag catgcttgac aagacagaag gcatcgtact
      481 ccgacaagtt agatatgcag aatctagcat gattatttcc atttttaccc aaaaatatgg
      541 tttgctcagt tttatggtgc aaggtgtgcg cagtaacaaa agcaaacaaa aaggcaatat
      601 gttgcaagtg ttgaatattt taatgctaga tatttacctc aaagaacaac gcaacttaaa
      661 tagaataaaa gaacacagcg cagcttatat ttataaaaat attccgaatg attttgcaaa
      721 gcaaagtatt ggtatttttt gtatcgagtt gatatcaaaa tgtataaaag aacaggaagt
      781 caacgaacga ttatatcaat acttagtagc atttttaatc gacttagaca acactaaaga
      841 gcgtatagaa aataagcctt tgtttttttt attggaaatg gcgagtattt taggcttcga
      901 gccctctttg caaaatatat tgcatggcat ttattttaat ttggaaagtg gcagatttga
      961 agacaacaac agtcatactc agaattgctt atcagcacat gaaacagaat tattgaaaaa
     1021 gttaatagcc atgtattatg aaaaaaacga ttggcaattt aacagtatgg aacgaaagat
     1081 tttgttggat aaattattgc tctacttccg ttggcatatt gctgaattta caacactaaa
     1141 atcgccggca gttctaagtg aaattttgag ataattgttg tgcttttata agccaccaac
     1201 ttttagtttt tctgcatttt cttcgaaagt aagcttatct atgatgtctt gtaaatcgcc
     1261 attcacaatc tccgataagt tatataaagt aagattgatt ctatgatccg taacacgacc
     1321 ttgcggataa ttgtaagttc taatttttgc acttctatca cctgtgctta cgagtgtttt
     1381 acgctgtttg gcaatcgctt cattgtgctc atttagtgct ttttcataca agcgtgtacg
     1441 caatactttc aatgcacttt cgtagttttt atgttgtgag cgaccatctt gacactcggc
     1501 gattatacct gtaggcaaat gcgttagacg cacggcagat tctgttttat taacgtgctg
     1561 tccgcctgca cctgatgcac gataaacatc ttttttaata tcagccatat tgatttcaac
     1621 gtccacttct tctgcctcag gcaatacggc cacagacgct gccgaagtat gcacacgacc
     1681 ttgcgattcc gtttcaggta cacgttgcac acgatgcacg cctgattcgt atttcagcac
     1741 gccatacaca ccatcgccag aaacttcaac cacaacctct ttataaccac ctgccgttcc
     1801 tgcattttcg ttcataattt cggttgtcca gcctttattt tcgcagtatt tcaaatacat
     1861 gcgtagcaaa tcgccggcaa atatactggc ttcatcgccg ccagttccag cacgaatttc
     1921 gagaatagca tttttttcgt cttccggatc tttcggtatt aatagatagc gaatttcttc
     1981 ttctaggttt ggcaattgtg cttgctgttc gtcgagctct gctttggcta gctcacgcat
     2041 ttctgcatct ttttcttccg ataaaatctg tttgttaaaa tcgatattgg aaagcaaatt
     2101 cttgtacgtc agataggcat ttacaattgg ctctaaatct ttatattctt tagaagtctt
     2161 tttaaatttt tgcatatcgc tcaccacttc agggtcgctt agttgcgatt ccaaatcttt
     2221 atatctgaaa taaatgagtt ctaacttatc taacataatt caaaaaatgc gtgcaaaaat
     2281 acaatattac tcgtgaattt cggcacgaaa gtgtgtattt gtataaaaag taattattat
     2341 attgtgttaa aatcataatt tatggattca caagatactg gctctttgac ggcaataatt
     2401 gctgcattag gcattttttt cttattcttt tttctattgt cgattttttt tatcgtttgt
     2461 cactggaaaa tatttaccaa ggctggtcgt aaaggttggg aatgtctcat tccattttac
     2521 aatctatatg ttcaattaca gattaccaag cagcctacta tttggttatt atttttcctc
     2581 ataccatttg taaatatcta ttttggtatt aagcacgtac atggactttc cttagccttc
     2641 ggaaaagatg taggctttac aattggcttg ttattcgtgc catttgtttt tattccaata
     2701 ttagcatttg gtagcgcaca ctacgtatat aatcaagatg aaaatctgtt gtttaatgag
     2761 atacaagaaa tcaaataagt tgaatgctag cgatagataa aaattttatt acactcataa
     2821 aatatcaagc ttttttatag tatcaatcga tttgtgactg attttcgtct tggtataata
     2881 attgtggttt aattaatttc tcattcctct atctgcttta tatgttagct tctgatgtaa
     2941 ccaatattca atattttgta caaatagtca aagaaattcg acctcacaaa aacaatcaaa
     3001 gcgaagtagc gaatcagcat atacaaacat ttattcatcg cttagaaaat aatgctacag
     3061 ccttacatga atttagaagt caatgtcaaa ttttaattca gcaatcgagc ttacaggaaa
     3121 tattttctga caacaacgat gtatttggca gtgtaaatat ttttgaacag ttagcaaaag
     3181 atattaaata tcgattctta gcgcccttaa tcgacagcag ttctttaaac tacaagctca
     3241 gcattatatt ttccaaaaag acagattaca tttggataaa ttcgatatgc gatgaacatt
     3301 ggattcgttt atttcaactt agtggaatcg acaatcaatt tgctgaagct ttcattttta
     3361 aaaaaataca gaatgccgct atgatgctgt cgtataaaat tgcagcatta ggtcttgaca
     3421 atgaattcct caacagattt gcgatggaag acgaattgat ttctccattt atagaacaaa
     3481 ataaagaact tgttttttat attgacaatt ttactactca cacatcagaa ttagcttcgc
     3541 ttagcaatac caacaaacaa atagttgtgt tattgcaaca atgcatggaa agtattcaaa
     3601 aaataagaaa gaacacaaca agctatggca cgagtttaag gcaaacctat ttactgaaca
     3661 aaactgctgt aaacattgag catttgaaaa ttttgctcga catttatgat gtcgcagatt
     3721 ttaatttaac acgattggta tgtttcttta aaaatgctgt ttacaatgaa aacaatagat
     3781 atagcattag gcacaccttg tcgaaaaaca taggccatct cgcttaccaa atagcagaac
     3841 ataaaggaac agtaggtgaa cattatatta cgagtaaaaa agaggaattt gttcatcatt
     3901 tcaaagccgc atttggtggt ggctttatca ttaccttgat ggtattcttg aaagtaggag
     3961 cttctacctt tcctattact gatttttgga aggccatttt atatagcatc atatatgcct
     4021 ctggctttgt gcttattcat ctacttggct ttacggtggc caccaagcag ccagcgatga
     4081 cagcatctgc attagcaaaa gaattagata aaaataaaaa tggaatcaat aacttgcaaa
     4141 atattgcttt gttaatggca caagtgagcc gcagtcaaat tattgccata tttgggaatt
     4201 tagcctccgt gtttcctgtt acactattta ttacttggct gctgcactcc tgctttcatt
     4261 ttgcggtaag cgatgccgac catgcaatgc attaccttaa agaaatccat cctttgtatg
     4321 cactaagtgc gtggtatgca gccattgctg ggttttattt gtttgtatct ggtatcatta
     4381 ccggctattt tgataatact gttatttaca gcagaattcc agagcgcata agacaaaaaa
     4441 ccacttggaa aaaattctta ggcgaaaaaa ggctggcgca attagccaat tatttacaac
     4501 ataatttagg ttcaataatg ggaaatataa tgattggttt tttcttagga acagcaacat
     4561 ttattggcac aacactaggt ttgccatttg acattcgaca cattaccatt tctacaggtt
     4621 tatttgccat agctatagaa actttacatt ttcaaatcgc attgaacatg ctatttggcg
     4681 tattgctcgg actatttggc atagcattat ttaatgtaat ttcgagtttt ggtttcgcat
     4741 tttatgttgc cttaaaatca aggaatatag acagtagcga attagcacaa ctgcctctta
     4801 ttttatggca atattttata aaacatccat cagattttat ttatccgcca aaaacagaac
     4861 gcacggttga aaatgtattt ggtaaagcag attaaaaacc cattgctttt ttccctttct
     4921 gtataatagt cgtatatcct tccggaagcg tgcgtgcaat attgtctaac aacttagcat
     4981 cagcaccaat taaaatgcgt gcgttattgc gttcaacacc gcgaatgatt gtttttgctg
     5041 cactttctgg cgatgtacgt tccaacattt tttggaatat gcgttcttca ttcgcatcgt
     5101 gtttgtaatg aactgcattc ttgtaaatat ttgtgtttat gccaccagga tgcacactat
     5161 gtacttgcac attggtatcg tacatttctg cacgcaatga ttctgtaaaa ccgcgcactg
     5221 caaattttgt cgtgcaatat ggcacttggt ctggcatgcc tattaaacca aaaaggctag
     5281 aaatattcac gattacggct tctggtcgtg ctattaatgt ttctaaaaat aattttgtat
     5341 gcaatacaac tgcatataca tttacatcca tcaatctatt aaaatcttcc atcttcacat
     5401 cgcggaaaga aatttttcct aatgcaaaac cagcattatt aaataataaa tcaatgtgtt
     5461 cgtgttcctt caacacttta gtacaaaaag cagtaactgc tttcacatca gagacatcaa
     5521 ctacataagt atggcattgt gcacctattg tttctactcg tttttttgta tctgctaatc
     5581 ctgtttcatt tttatctgat aacactaaaa ttgcgccttt cttcgctaat tcaatggcta
     5641 atgcttgtcc aatacctgag cccgcaccag taattacggc tattttgtct ctgaaatttc
     5701 tcatttttta aaaatatttg tcacaaggta ttgattcaag tcaattaatg cactttacaa
     5761 aggtgaaaaa tgacaatttc gtattttttt actaatttta acatgccgat aaacaaatag
     5821 gagcgatatt tgtatataca attataaatg tcaacaatga atttaagatt tttttctata
     5881 gcaagtattt tactttttag tgcgtttaca ctattcgcta aaaatgatgt tgctgtaaaa
     5941 gaaacgacaa tcaatgcgca aaaaagtagc attgagtgga ttggcaaaaa agtactcagc
     6001 caacatagtg gcaaattaaa tattaaaagt ggtgcactta gcacaaagaa tggaatatta
     6061 ggcggtggca cattcacaat cgatatgaca tctatcactt gcgacgacat taaagatgct
     6121 gattacaata aaaaattagt gggtcactta aatagtactg atttttttaa tgttgcggag
     6181 catccaacag caatagttaa gataacaaaa gtgttaaaaa ttaataataa agctaatgcg
     6241 tacaacctta ctgctgattt aacaatcaaa ggtattacca atagtatcac atttccagcc
     6301 acttttgaaa agaatggaaa tgtatttgaa ggaaatgcaa acatcactat agatagaacg
     6361 ttatgggaca ttaagtataa gtccgctaac ttcttcgaag gacttggcga caaagccatc
     6421 aagaacgaga ttgagttgaa agtgaatatt gtatctaatt aattttgaac tctattgatt
     6481 gccgagaggc aattgggttt ttcatagttt tatggttttt gtggcttgtc cgatcctttg
     6541 ggtcggacaa gtcattattt agactaagtg tttttgaaaa aaatctaggt taaagctgct
     6601 gtaaatccac taatttttta tactcgcctt gcaattgcag tagttgttga tgcgttccgc
     6661 tttcgataag ctcaccttct tttaatacca aaatttcatc tgcaagttgt actgtagaaa
     6721 gtctatgtgc aataatgatt gcagttttat tcttccataa attttgcaag gcatcttgca
     6781 ctaatttttc tgaattgcta tccaagtggg aagttgcttc gtctaaaatc aaaatgggtg
     6841 cgtctttgta gattgcacga gcaatagata aacgttgttg ttcgccgcca ctaatcttag
     6901 tgccattatc accgattaat gaatcatact gctgaggcat tttctgaata aaaatatctg
     6961 cgtgtgcctg tttggctgcg tgcacaatct gtggctcatt tacgagcaaa ccgaaagcaa
     7021 tattattgct tactgtatca ttgaacaatg tagtgtgctg cgttaccaaa gccatttgac
     7081 ttctgagcgc ttgctgctta tagtttttta tattaacgcc atcaattaaa atttcgcctg
     7141 aatcaatgtc aaaaaagcgc aacaacaatt gaataaatgt tgactttccg gcaccagaaa
     7201 caccgactat cgccactttt ttacccttag gtattgttat agaaatattt tttaaaaccg
     7261 ttctttcatg gtaagcaaaa caaacatttt tgaactctat tttatcttta aaatctaata
     7321 atggcaatgc ttgtgcgata tcatttactt tattttcttc ttgaagcact tcttcaattc
     7381 gtctaagcga tgccatgcct ttctgtataa aataaaatgc atttgaaaat gcttttgctg
     7441 gttgtataat ttgtgaaaaa atcacaataa aggcaataaa agcctctgca cttagtggcg
     7501 atgtatgatg aataatcatt ttgccgccaa catataacac cactactacg acgctaatgc
     7561 ctaaaaattc agacaatggc gtggacaaat ctcgcttgcg tatcatactt gtacctactc
     7621 tgttgtgctg ttcgttgata tttctaaatt tattagaaaa actttgctca gcggtaaaag
     7681 attttattac tctaatacca gaaattgttt cgtcaacgat agaattgagt acaccttgta
     7741 aatgttgcgc tgtcgaggaa gcacgtttta atgttttacc tattttacca ataaaatagc
     7801 cagaaagcgg caagataatc aacacaaaca aggtaagttt tggactcaaa attagcatgg
     7861 taagcaaggt agcaagtatt gttactggct ctttgaatgc tacttctaca aaatgcaaaa
     7921 tgccgtattc aacttcttgc acatcattcg ttaatcgcgt aagcacatct ccttttcgct
     7981 tatcagaaaa atataagacc tgcaagtgta gaattttatc gtataattct tgtctaatag
     8041 tgctggcaat acctgtacgc atcgaagcta aaaaatgaac tgctgaatat cgaaatacat
     8101 ttttgacaag aaatacaaaa acagtaaata ggcaaataaa taacaacgcc tgtgacttgc
     8161 ctgcttctat cgtgtcagca ctttgtatat attgaataac tttattgtta aagcttgttc
     8221 caataaattc ttttaatgaa gtaataccaa acgaagatgc ctccacatgc tgcaacacca
     8281 tcttatctgc attaaaaatg agatttaaaa aaggaattaa catcaaaaaa gaaaaaatcg
     8341 aaaagatggt ggaaagtaag ttaaatatta tattgagtgc aatatttgga atatatggct
     8401 ggcaatacct taataaacta cgtaagtcct tcattatagc aaagatatta tttacttttg
     8461 ttttatggaa tctataaaac aaagacaagt agccaaaacc atacaaattg ccatgagcga
     8521 ggtggtgcag aaagatttag gtggcatttt gggtggcgcg atgctcacca tttcgggcgt
     8581 gcgtgttaca cctgatttat atactgctcg aatttatgta agtatttaca accacgctaa
     8641 acctgatacc gttttagaca atctcgataa aaacaataaa atgattcgag gactgctagg
     8701 caataaaatt aaaaacaaag tacgctctat tccgcaatta gagtttttta aagatgacac
     8761 tttagaagat gtccaaaatt tggagcgcat ttttagtgaa ataaaaaaga aagatgctga
     8821 aattgagaaa atgagaggaa aaaatgatga aaacgaacag taggtaatgt aaataaacga
     8881 atttgaatct cgaactcaac atagccaaac gatacttatt tagtaaaaaa tctaccaatt
     8941 cgattaatat catctcattg gtaagtggtg ctgctatgtt gtttggaacg atggccttga
     9001 ttttagtact ttctgttttc aatggcttag aagatttggt aaaatcatta tatgctgtat
     9061 tttatcctga cattgccatc actgtgaaaa ctggcaaaac gtttgaaatt acaaatgagc
     9121 ttcgccaaca actcaatact atcgactaca tcgatgcatt tagttttacc ttagaagaaa
     9181 atgcactttt agaatatgct gaacaacagc atatttgcac agtcaaaggc gtggacaaaa
     9241 attattttaa agtcatcaat aaatttgata cgtttattat tgcaggcaaa aaaatattac
     9301 aaaaagacag tatcaacttt ggaattgttg gcgttggcgt ggcacagaaa ttaggattag
     9361 atccaacaaa tgcctttcta ccacttacac tatacatgcc taaaaaaact gcatcttctt
     9421 tttcatcgat ggagaatgct tttaacaaaa gctatgtcac accaagcgct gcttttgccg
     9481 tttctgatga atttgacaca aaatatacat ttgtgccact cgattttttt cagcaactca
     9541 cagacaattt tggtcgagct tctcaaattg acattcgatt aaaaaaccag actcagagtg
     9601 cacttgcaat tgctgcaatt cagaaaaaaa taggaaataa ctttatacta aaaagtcgct
     9661 acgaacaaaa cgaagtttta tataaaatac taaaaagcga gaaatggatt actttttcaa
     9721 ttttattatt aatcgtgatt attgcttcat ttaatattat tggagcctt
//
LOCUS       DAOQQV010000098         9609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2643_length_9609_cov_6.881097, whole genome shotgun sequence.
ACCESSION   DAOQQV010000098 DAOQQV010000000
VERSION     DAOQQV010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2643_length_9609_cov_6.881097"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3342
                     /locus_tag="PLL59_09685"
     CDS             <1..3342
                     /locus_tag="PLL59_09685"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY24120.1"
                     /translation="GNNIIRWYGNGIDSLSLIEFSSDNGANWQTVVNNKMLSAKTHTW
                     NVPATPSTQCLIRITSGNNVDVSAANFTIGAQLNYNLITAAPCDQSVRISWAAFPGAS
                     AYRVYIFKDTAWSLVGETAQTSLNVTNLINGKKYAYAVSTIYNGFEGNHSLAKFFKPT
                     ATACLTANDVGVYQVDKPKGGRLLTSTALSNAEKISLIIKNFGTTTQNAINVSYRING
                     GAIRNATLTDVITSNDTSIIKFNVNENLSAIGNYNLVAWTNLIGDENTANDTLYYTIK
                     QLQNAPIVLPFSESFESTNVQYSKPVFGINGIEYTDYYPELGAMLRANEGNLYAHTGE
                     HAITLDNYLGAGLKKNEFIFTVNLSNYVDSIIFLSFNYLNRAEADSNDILYARGNDTQ
                     AWIPIFDLFANRGNAGEYKKVDEINLYQKLKIENGQNFSTSTQLKIQQIGTKTAITPY
                     ADGGYTFDDFLLQVAGKDVALSHADIKKANCKKSFSAQPVSVTIKNNSAATITNLPVF
                     YQAEGQAVVSEIISTSIAANDTLQYTFNTLFNTSTAGLFPIKVWANNNGDKYPSNDSA
                     TTSVVVMETIDNFPYYNDFENNSGPFLSEGTNNSWLWTTPNKYNISYAAQDNKAWTTG
                     ASNGYNFNENSYLYLGCLDFSSLSIDPYIAFNFISVMHTQSDSAYVEYSTDGNVWSRL
                     GCYNCGVNWYNGFSGKPYWDRVVFPWQVAHIKVPLADLQDASNFMYRLHLLSDDYIVS
                     EGIGIDDIRIFNDYTDIATTDSTYIIAPSSANGWLSFYRNGRLVAELFDDNKNLGNVS
                     LGYEANMDKHKTLDNKNAFPRNWVVKPQNAQIGNYKLRLYVLNTEYTEFTINEDSISR
                     MGDIGLLRYFGLNTNLDIIDNHVRAYYKYLSPSQIQFYPYQNGYYVEFETDTLGEFYL
                     MSTKQDADAIQNINLLDFSANKINDDVLLEWKTSREVNSRDFIIQYSFDASVFIDVDT
                     IPAGGFSNILTPYNYLHELNASSGVFYYRIKMIDNANKISYSLIDSVYFAPTVGVKNH
                     EYLANAYVAENDIVVELKNKTQLPAQVHIYNTNGQLQFAKKMTLSNGKNELGIANFAS
                     WSNGIYFLQIRNEKQNYYTKLLIQH"
     gene            3342..4016
                     /gene="mnmD"
                     /locus_tag="PLL59_09690"
     CDS             3342..4016
                     /gene="mnmD"
                     /locus_tag="PLL59_09690"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HMY24121.1"
                     /translation="MERSVVLTADGSSSVFVPHFDEYYHSVHGALQESVHVFIDAGFL
                     FKINNSKELSILEIGFGTGLNTFLTFLEAEKYNAKIDYIAIEKFPLERNIYEALHFNT
                     ITDDANKQARFMSMHTLEWNKKSKVNTHFSLTKLHIDVLNFKASNRFDIIYFDAFAPT
                     AQKELWTTAVFTAMYDALKNEGVLVTYCAKGEVKRILKSVGFVVQSLPGPIGKREMTR
                     AIKLNK"
     gene            4013..5062
                     /locus_tag="PLL59_09695"
     CDS             4013..5062
                     /locus_tag="PLL59_09695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMY24122.1"
                     /translation="MKEVDYIIVGQGIAGTMLANQLLHDNLSICVIDQFEPNASSNIA
                     TGVVNPVTGRKMVKSWMIDEVLPFAKKTYRSLESLLQYSFFHDKEIYKVFSTVEDVQI
                     WNTKKNDSAYANYLGDIISSQEANISIEAPFGIGIIKNACWLDVPLMTKLFRHYLLKK
                     EVLIEAHFDIDLLEISTHICYKNIKAKGIIFCEGYRACNNPYFNWIPFTLAKGEQLLI
                     HSDDLALTSILNKNIFLVPKGNAFYSVGSTFIWNDTTETVTADGRKEITDKLSKITSA
                     SYVIIEEKAGIRPTMRDRRPVIGQHPIHSNIFIFNGLGTKGVSLSPYFAQHFSGYLRG
                     NNELLQEISLQRLVE"
     gene            5105..5734
                     /locus_tag="PLL59_09700"
     CDS             5105..5734
                     /locus_tag="PLL59_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24123.1"
                     /translation="MSKFDFLKSWWKAVPLLLLLSIIAIYKFRYTLNLVKPEVEVVEA
                     IDSSYLLNKNKQEIIARGTIMEIPFHGIDATRYPIDHPMDSGMDLITNDRFYIIKLYD
                     PSTKIEIYIAIRDLALLDKLPFSYSFDTGEKILVTAFIRNKSGVNAMYGGTIHNNRGK
                     LKLVSYNRVTNTATGELDAELDGVIENALCEVKGLKFNNAILQYNKINN"
     gene            complement(5731..7554)
                     /locus_tag="PLL59_09705"
     CDS             complement(5731..7554)
                     /locus_tag="PLL59_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HMY24124.1"
                     /translation="MSFRKPSKDNSKTDKFTKDTFLSGIKILSYLKPHKFLFFLGAFF
                     LIVSTVTALSFPYLFPLLVDLAQHKEVHLIPYNGTTLINITSRLELIEIIVALLIIQS
                     IVSFLRLYIFAHTNERILADIRYDLYTKIISLPISFYEKNRIGELTSRITSDVTKLHD
                     VLSIDIAEFIRQIFTLLGGIVLLFLISKELTLFILYTVPVVVVFGLFFGKFIRKKSAE
                     AQQAIADSNVVVEETFHNINIVKAYTNELFESNRYLQKVNKIKNVNLGNAVFRGLFIS
                     FIILVMFGVITLVLYKASGLLEVNKLTVGQVIQFIIFTVFIAGSLAGIADLFGKIITA
                     LGASDRITQILAIPSELDIKEAHANKINGVIEFNDIHFHYPTRPEIEILKGISFKANK
                     GEKIALVGSSGAGKSTILQVLLRFYAIQKGSITIDNISTTEMNIQDLRANIAVVPQEV
                     LLFGGTIKENILYGKLGASDEEVMHAVIKSNSLEFIERFPEGLNTIVGERGVKLSGGQ
                     RQRIAIARAILKDPAILLLDEATSALDAASEKLVQSALDTLMEGRTTIIIAHRLSTIR
                     NVDKIIVINHGKIAEQGTHQELSMMQDGLYNHLLKLQYQLH"
     gene            complement(7664..8884)
                     /locus_tag="PLL59_09710"
     CDS             complement(7664..8884)
                     /locus_tag="PLL59_09710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMY24125.1"
                     /translation="MDFKEFGFDERLLEGIDAMNYKTATPVQEQVIPIILAGKDVIAA
                     AQTGTGKTAAFLLPILNELITQEKHEDEVNAIVIVPTRELAVQISQNLEAFSYFTPIS
                     SIAVYGGSDGSSFETEKRALSHGVDIVICTPGRMISHLASGYVRLKGLKHLILDEADR
                     MLDMGFYEDIIKIIKYLPTERQTLLFSATMPPKIRQLGLSILKDPAQINISISKPPEK
                     ITQLAYIIFEAQKTPIIQSILKDVKFKKIVVFCSRKENVKKLSRELNRSGIKNEEIHS
                     DLEQQMREQVLLNFRSGKIAIIIATDVISRGIDIEDIDLVINYDVPNDGEDYVHRIGR
                     TARAATDGTAITLVSEPEQYKLFLIEELLAYPIPKAAVDTQFGETPLYQPQPKKKLKK
                     PNKRRHFRKKGPQQ"
     gene            complement(8980..>9609)
                     /locus_tag="PLL59_09715"
     CDS             complement(8980..>9609)
                     /locus_tag="PLL59_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY24126.1"
                     /translation="SCDTVFTEFHSESCFISGVTVIKDTFQTSLGCDSIVTTTITLKP
                     SPDASFTYTLLGNVLTLTNTTTNGGLCLWEFGDDSISTEISPTHIYQNPGTYTIKLTT
                     ANYCSADTFSTTINVLSTDLHHTKKDKQVQIYPNPTKEHFTLQIEINNAENIRIEVQN
                     LLGQTVLSQQLTTGNTHEINLNTLITSGMYYINLIKDNNTIGIYKLLVK"
BASE COUNT         3205 a         1557 c         1645 g         3202 t
ORIGIN      
        1 ggaaacaaca taatacgatg gtatggaaat ggcatagact cattgtcatt aatagaattt
       61 tcatcagaca atggcgcaaa ctggcaaaca gtagtgaata ataaaatgct gtctgcaaaa
      121 acgcatacat ggaacgttcc ggcaacacct agcacacagt gtttaattag aattacatct
      181 ggaaataatg ttgatgtgtc agccgcaaat tttacaattg gcgcacagtt aaattataat
      241 ttaattactg cagcaccatg cgatcagtct gtaagaataa gttgggcagc ttttccaggt
      301 gcatcagcct atcgcgttta tattttcaaa gatacagcat ggtcattagt tggcgagacc
      361 gcacaaactt cattaaatgt aactaatctt atcaatggaa aaaaatatgc atatgcagtt
      421 tctactattt ataatggttt cgaaggcaat catagcttgg caaaattctt taaaccaaca
      481 gcaacggctt gccttactgc taatgatgtg ggcgtttatc aagtagataa acccaaaggt
      541 ggtagattgc ttacttctac agcacttagt aatgcagaaa aaatttcgct tatcatcaaa
      601 aactttggaa cgactacgca aaatgcaatc aatgtatcgt atcgaatcaa tggcggcgca
      661 attagaaatg caacacttac agatgtcatt acaagcaatg atacttctat aataaaattt
      721 aatgtaaatg aaaatttatc agcaatagga aattacaatc ttgtagcatg gacaaattta
      781 attggcgatg aaaatactgc aaacgacaca ttgtattaca cgattaaaca attgcaaaat
      841 gcaccgatag ttttgccatt ttctgaatct tttgaaagca caaacgtgca atatagtaag
      901 cctgtttttg gtataaatgg catagaatac acagactatt atcctgaact tggtgctatg
      961 ttgcgtgcca atgaaggcaa tctgtatgca cacacaggcg agcacgcaat tacattagat
     1021 aattatttgg gagcaggtct taaaaagaat gaattcatct tcaccgtgaa cttatcgaac
     1081 tatgttgatt ccattatttt cttgtctttt aattatttaa atagggcaga agccgatagc
     1141 aacgatattc tatatgctcg tggaaatgat acgcaagctt ggattccgat ttttgattta
     1201 tttgcaaata gaggcaatgc aggcgaatac aagaaagtag atgaaattaa tttgtatcag
     1261 aaattaaaaa tagaaaatgg acaaaatttc tctacatcta cacaattaaa aattcaacaa
     1321 attggcacga aaacagcaat tacgccttat gcagatggtg gctacacttt tgatgacttc
     1381 ttgctgcaag ttgctggaaa agatgttgca ctttctcatg ctgatatcaa aaaagcgaat
     1441 tgcaagaaaa gcttttcggc tcaaccagtt tctgttacta ttaaaaataa ttctgctgca
     1501 acaatcacaa atttacctgt gttttaccaa gccgaaggac aagcagttgt atcagaaatt
     1561 attagcacaa gcatagcagc caatgataca ttgcaatata cttttaatac actttttaat
     1621 acgagtactg ctggcttgtt cccaataaaa gtatgggcaa ataataatgg cgataagtat
     1681 ccttcaaatg attctgctac cacatctgtt gttgtgatgg aaacgataga taattttcct
     1741 tattacaatg atttcgaaaa taatagtggg ccatttttgt cagaaggaac aaataattct
     1801 tggctatgga caacacctaa taaatacaat attagttatg ccgctcaaga taataaagct
     1861 tggacaactg gcgcaagcaa tggctataat ttcaatgaaa actcctattt gtatttaggt
     1921 tgcttagatt tctcttcttt atcaatcgat ccatatattg cttttaattt tatatcagta
     1981 atgcatacgc aatcagattc tgcttatgta gagtattcaa ctgatggcaa tgtatggtca
     2041 agattaggtt gttataattg tggtgtaaat tggtataatg gtttttctgg aaaaccttat
     2101 tgggatcgcg tcgtatttcc ttggcaagtt gcacatatca aagtgccatt agcagactta
     2161 caagacgctt ccaattttat gtatcgttta catttgctct ctgatgatta tatagtttca
     2221 gaaggtattg gcattgatga tattcgcata tttaacgact atacagatat tgcaacaacg
     2281 gattcaacat atattattgc gccttcatct gctaatggct ggttgtcgtt ttatcgcaat
     2341 ggaagattgg tggcagagtt attcgatgat aataaaaatt taggaaatgt ctctttaggt
     2401 tatgaagcca atatggataa acataaaaca ttagataata aaaatgcctt tccaagaaat
     2461 tgggttgtta agcctcaaaa tgctcaaatt ggaaattata aactacgctt atatgtactc
     2521 aatactgaat acacggaatt tacgattaat gaagattcta tcagtagaat gggtgatatt
     2581 ggcctgttgc gctattttgg cttgaataca aacttagata taatagacaa tcatgttcgt
     2641 gcttattata aatatttatc accatctcaa atccaattct atccttatca aaatggatat
     2701 tatgtagagt ttgaaacaga cacattaggt gagttttatt taatgagcac aaaacaagat
     2761 gctgatgcta tccaaaatat taacttacta gatttttcag ctaataaaat aaatgacgat
     2821 gtgttattag aatggaaaac ttcgcgagaa gtcaacagta gagattttat catacaatat
     2881 tcattcgatg cttctgtttt tattgacgtt gatacgattc cagcaggcgg attttcgaat
     2941 atactcacac cgtataacta tttgcatgaa ttaaatgcca gcagtggtgt gttttattat
     3001 agaatcaaaa tgatagataa tgcaaataaa ataagctata gcttaataga ttcagtttat
     3061 tttgcaccaa cagtaggtgt aaagaatcac gaatatcttg ctaatgcgta tgtggcagaa
     3121 aatgatattg ttgtagaact gaaaaacaaa acgcaattgc ctgctcaagt ccatatatat
     3181 aatacaaatg gtcaattgca gtttgctaaa aaaatgacat tatcaaatgg gaaaaatgaa
     3241 cttggtattg ctaattttgc atcttggagc aatggaattt atttcttgca aataagaaat
     3301 gaaaaacaaa attattacac taaactttta atacagcatt aatggaacgc agtgttgtgc
     3361 taacagctga tggtagctct tctgtttttg ttccacattt cgacgaatat tatcactctg
     3421 tacatggtgc cttgcaagaa tcggtgcatg tttttattga cgccggtttt ttatttaaaa
     3481 taaataattc aaaagagctt tctattttgg aaattggctt cggtacgggt ttaaatacgt
     3541 ttcttacatt tttagaagca gaaaaatata atgctaaaat tgattatatc gcaatcgaaa
     3601 aatttccatt agaaagaaat atctacgaag cacttcattt taatacaata acagatgatg
     3661 caaataagca ggctcgtttt atgtcaatgc ataccttaga atggaataaa aaatcaaaag
     3721 taaatacaca tttttctttg accaaacttc atattgatgt attgaatttc aaagcaagta
     3781 ataggtttga tataatttat tttgatgcat ttgctccaac agcacaaaaa gaactttgga
     3841 cgacagctgt ttttacagcc atgtatgatg cattaaaaaa tgaaggcgta ttagtaacct
     3901 actgcgcaaa aggtgaagta aaaagaatat taaaaagtgt aggatttgta gtgcaatcac
     3961 tgccaggtcc aatcggcaaa cgcgaaatga cacgtgccat taaattaaat aaatgaaaga
     4021 agttgattat atcatcgttg gtcaaggtat tgcaggcact atgcttgcca atcaattatt
     4081 acatgataat ttatccattt gtgtaattga tcaatttgaa cctaacgcat catctaatat
     4141 tgcaactggc gttgtaaatc ctgtgactgg tagaaaaatg gtgaagagtt ggatgattga
     4201 tgaggtgttg ccattcgcaa aaaaaacata ccgttcatta gaatctttat tacagtattc
     4261 attttttcat gataaagaaa tctataaagt gttttcaact gtagaagacg tgcaaatatg
     4321 gaatacaaaa aaaaatgaca gtgcgtatgc aaattattta ggagatataa tttcaagcca
     4381 ggaagcaaat atttccatcg aagcaccttt tggaattggt atcatcaaaa acgcatgttg
     4441 gttagatgta cctttgatga caaaattatt tagacattat ttactaaaaa aagaagtatt
     4501 gatagaagcg cattttgata tcgatttatt agaaatttca acacatatct gctataaaaa
     4561 tatcaaagca aaaggcatta tattttgcga aggttatcga gcatgtaata atccatactt
     4621 taattggata ccatttacgt tggcaaaagg tgagcaactg cttattcatt ctgatgattt
     4681 agcattaaca tctattctca ataaaaatat ttttttagtt ccaaaaggaa atgcatttta
     4741 tagcgtaggc tctacattta tttggaatga cacgacggaa acggtaactg ctgacggcag
     4801 aaaagaaata acagataaac tttcaaagat tacatcagca tcttatgtca ttatagaaga
     4861 gaaagctggc atacggccta ctatgcgcga cagacgacca gttattgggc aacatccaat
     4921 acattcaaac atttttatat ttaatggact gggcacgaaa ggtgtttcat tgtcgccata
     4981 ctttgcacag catttctctg gatatttacg tggaaataat gaacttctgc aagaaatttc
     5041 gctacaacga ttggtggaat aaacactaat caatatcttt gtaagtattg taaatgataa
     5101 acctatgagt aaatttgatt ttttgaaatc atggtggaaa gcggtgcctt tgttattgtt
     5161 attaagcatt attgccatat acaaatttag atatacatta aatttagtaa agcctgaagt
     5221 cgaagttgtg gaagcaatag actcgagcta tttacttaat aaaaacaaac aagaaataat
     5281 tgcacgcggt accattatgg aaattccttt ccatggtatt gatgctacac gctatccaat
     5341 cgaccatcca atggacagtg gtatggattt aatcaccaac gatagatttt atattatcaa
     5401 actgtatgat ccaagtacaa aaattgaaat atatattgcc attcgtgatt tagcactatt
     5461 agataaattg cctttttctt attcatttga taccggagaa aaaatattgg taacagcatt
     5521 tatcagaaat aaatcaggcg tgaatgctat gtatggtggc accatacaca acaatcgagg
     5581 caagctaaaa ttggtatctt ataacagagt aacaaatact gctactggcg agttagatgc
     5641 agaattagat ggcgttatcg agaatgcttt atgcgaagta aaaggcttga agtttaacaa
     5701 tgccatctta caatacaaca aaattaataa ctagtgaagt tgatattgta gtttcaacaa
     5761 atgattgtat aaaccatctt gcatcatgct taactcttga tgtgttccct gctccgcaat
     5821 ttttccatga ttgatgacaa ttattttatc gacatttcta atggttgaca gtcgatgtgc
     5881 gatgatgatt gttgtacgtc cttccattaa tgtatctaaa gcactttgta ctaatttttc
     5941 tgatgcagca tctaatgcac tagttgcttc gtctaataat aaaatggcag gatctttcag
     6001 aatagcacga gcaatggcaa tacgctgacg ctgtccgcca gatagcttaa caccacgttc
     6061 gccaactatt gtatttaaac cttcgggaaa acgctcaata aattctaaag aatttgattt
     6121 tattacggca tgcattactt cttcatcgct tgcgcctaat tttccatata gaatattttc
     6181 tttgatggtg ccaccaaata ataaaacttc ttgtggcact actgctatat tcgctcgcaa
     6241 atcttggata ttcatttctg tagtagaaat attatctatc gtgatgctac ctttttgaat
     6301 agcgtagaat cgcaataata cttgtaaaat tgttgacttt cctgcacctg acgaacctac
     6361 taatgctatt ttttcgcctt tattagcctt gaatgaaatg ccttttagaa tttctatttc
     6421 aggtcgcgta ggataatgga aatgaatatc gttaaactca ataacaccat ttattttatt
     6481 tgcgtgtgct tcttttatgt ctaattctga tggaatagct aaaatttgcg taatcctgtc
     6541 tgatgcacct aatgcggtta taattttccc aaacaaatca gcaatacctg ctaatgaacc
     6601 agcgataaaa acagtgaaaa ttatgaattg aattacttga cctaccgtta atttgttgac
     6661 ttccaataag ccactcgctt tgtataatac taaagtgatg acgccaaaca tcactaatat
     6721 aataaaggaa ataaataaac ctctaaaaac agcattgcct aagtttacat ttttaatttt
     6781 attgactttt tgtaaataac gattgctttc aaacagctcg ttggtgtatg cttttacaat
     6841 atttatatta tgaaaagttt cctcgactac aacatttgaa tcggcaattg cttgttgtgc
     6901 ttcagcagat tttttgcgaa taaattttcc aaaaaataag ccaaatacaa ccacaacagg
     6961 aactgtgtac aatataaata aagtcagctc tttagaaatc aaaaacagta acacgatgcc
     7021 acctaataag gtgaaaattt gtctgatgaa ttcagcaata tctatggata atacatcgtg
     7081 caattttgtc acatctgagg tgatgcgact tgtcaattca ccaattctat ttttttcata
     7141 aaatgaaatg ggcaaactaa taattttggt gtataaatca tatctgatat cagccaaaat
     7201 acgctcattg gtatgtgcaa aaatgtataa gcgcaaaaaa gaaactatac tttgaatgat
     7261 taacaaggca acaatgattt ctatcaattc taaacgtgat gtaatattga tgagtgtcgt
     7321 gccattgtat ggtattaagt gcacttcttt gtgttgtgct aaatcgacaa gtaatggaaa
     7381 taagtaggga aatgataaag cagttacggt agatactatc aagaaaaaag cacctaagaa
     7441 aaatagaaat ttatgaggtt ttaaatagct taatattttt atgccgctca gaaatgtatc
     7501 tttagtaaac ttatctgttt ttgaattgtc ttttgaaggc tttctaaaac tcattttttt
     7561 tatttaaaaa tagaattaat aatgacagtt ttttttattc agcatattga tttcacatta
     7621 tttaataaaa tcaatttaat gcaataatta taagttaacc ttattattgc tgtggccctt
     7681 tttttctaaa atgcctacgc ttatttggtt tcttcaattt cttttttggc tgtggctggt
     7741 acaatggtgt ttcaccaaat tgtgtgtcaa ctgctgcttt aggaatagga taggcaagca
     7801 attcttcgat caaaaaaagc ttgtattgct ctggctcact caccaaggtg attgctgttc
     7861 cgtcagtggc tgctcttgct gttctgccaa tgcgatgtac ataatcttca ccatcattcg
     7921 gaacatcata atttatcacc aaatcaatgt cttcaatatc gattccgcgc gatatcacat
     7981 cagtcgcaat aataatggct atttttccac ttctaaagtt taataatact tgctctcgca
     8041 tctgttgctc taagtcggaa tgaatttctt catttttaat tccgcttcta tttaattcgc
     8101 gacttagttt ttttacgttt tcttttcttg agcaaaaaac aacaattttt ttaaacttca
     8161 catcttttaa aatggattgt atgatgggcg ttttttgtgc ttcaaaaatg atataagcta
     8221 attgcgtaat tttttcaggt ggttttgata tcgaaatatt tatttgcgct ggatctttca
     8281 aaatacttaa gcctaattgc cttatttttg gtggcattgt agcggaaaag agtaaagttt
     8341 gtctctctgt tggcaaatat ttgatgattt taataatgtc ttcataaaaa cccatatcca
     8401 acattctatc tgcttcgtct aatatcaaat gttttagtcc tttaagtcgt acataacctg
     8461 aagctaaatg gcttatcatt cggcctggtg tacaaattac gatatcaacg ccatgtgata
     8521 atgctctttt ttctgtttca aagctagagc catcgctgcc accatataca gcaatggagc
     8581 tgattggtgt gaaataagaa aacgcttcta agttttgtga aatctgaact gctaattcgc
     8641 gagtaggcac aatgacaatg gcatttactt catcttcgtg tttttcttgt gtgataagct
     8701 catttaaaat tggcaataaa aaagctgcag tttttcctgt tcctgtttgc gcagcagcaa
     8761 tcacatcttt tcctgcaaga ataatcggta ttacctgctc ttgtaccggc gtagccgttt
     8821 tatagttcat ggcatctatt ccttccagca aacgctcatc aaagccaaat tccttaaaat
     8881 ccaatgatag tatttttatt taagcatcaa agatacaata tattatgcgt tttgcgaatt
     8941 tcaatcgcac aaggaatgtc gatatagtga taaataagct tatttaacta gcaatttata
     9001 tatgccaatc gtattgttgt cttttattaa attgatataa tacattccgc ttgtaataag
     9061 tgtgtttagg tttatttcgt gtgtatttcc tgttgttaat tgttggctta atacggtttg
     9121 acccaatagg ttctgcactt caatgcgtat attttcagca ttatttattt caatttgtaa
     9181 agtgaaatgt tcctttgtag gattaggata aatttgtact tgtttgtcct tttttgtatg
     9241 gtgtaagtca gtgctcaata cgtttattgt agttgaaaat gtatcagcac tgcaatagtt
     9301 agcagttgta agttttatcg tgtatgtgcc tggattttga taaatatgtg taggtgatat
     9361 ttctgtggaa atactgtcat ctccaaattc ccataagcat aaaccaccat tagtcgttgt
     9421 gttagttaaa gtcaacacat ttccaagaag tgtataagta aaagaggcat ctggactggg
     9481 ttttaaagta atagttgtag taacaatgct atcacaacct aagctggttt gaaatgtatc
     9541 ttttataaca gtcacacctg aaataaaaca agattctgaa tggaattcag taaatacagt
     9601 atcgcaact
//
LOCUS       DAOQQV010000099         9595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2648_length_9595_cov_6.077987, whole genome shotgun sequence.
ACCESSION   DAOQQV010000099 DAOQQV010000000
VERSION     DAOQQV010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2648_length_9595_cov_6.077987"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="PLL59_09720"
     CDS             <1..1435
                     /locus_tag="PLL59_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24127.1"
                     /translation="LETANNVITETTSESSSINTIEQTTSTANIEEKIENNIEIENVA
                     EVSNTNTVEVNDINEEKTDETSLIISAEETVETEEKEPIKDIIIVDTAARTINTQSIE
                     VTDAPNEQDISVDNTSVEEVMEIKVVDAKTIEKTNEEEDNTAEDTKNIISEISNNIEI
                     ESTEKIAAVNTEQAISITQLNNFDDEHETALYPEPLLVKVEPVFAVTQKEEDKETVIF
                     ENKTTNNKVDETNYKETSSTTAENINTTPNIEIAVVEKTESNIEEKITLEENNETIIE
                     QLEKQSSVLESESALFIPEVEEDLVEEHVPLEIVTDKENIVTSPIHEPSIDDEIIPKI
                     IDEEVKSEPHTFVEWLKILDGNLQIQTTESVKEAEHWIEIPRYEVEQTLAHKKEIAKE
                     EQKLFEPNFEEGEVDLFNEIDEEVSKVATESVVFKQDMMTETLAKIYHKQGKLDKALE
                     IYNTLRLKIPEKSAYFASLIEKIEKEK"
     gene            1437..1796
                     /gene="secG"
                     /locus_tag="PLL59_09725"
     CDS             1437..1796
                     /gene="secG"
                     /locus_tag="PLL59_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HMY24128.1"
                     /translation="MFTFLTILIIIVSLFITLIVLIQNPKGGGLSGGIGSAASNVIGV
                     QSAGDVMEKTTWGSAIVLLALCVMTAFVVPKEGAADKVKTLTEGAAKSAPSVPTTPTT
                     PAAPQNNQPANNPLAPK"
     gene            complement(2068..3387)
                     /locus_tag="PLL59_09730"
     CDS             complement(2068..3387)
                     /locus_tag="PLL59_09730"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HMY24129.1"
                     /translation="MITKLNKLMMMLTMIVVLSSVTSCRKDKIDEPPINTVDPNLPTS
                     SIADLKALFTSGLPITITDDITISGIVVADDKSGNYYKTIIIDDGTAGIPVLIDRSGL
                     YNDFPVGRKIYIKCKGLVLGEYNKFLQLGGFIDNSDGQPSVGSIPSSLITKYIVKGPS
                     GNAVTPIKINKLSELTKAFQARLIEIDPASFQASDQDKPWANIITQQSLSRIATDCFG
                     DNIEVRTSNFAKFANQNTPIGNIKLIGVYSVYGSTKQLTIRDAAELIPATAACPVEVV
                     LFSEDFNSASTSGNLSLPNWTNFSTVGSKYWYATGSSNKNARISAYNSGEASNIGWLI
                     TPLIDLSSSTNETLNFRTNVFSPAGSTTLEILYSIDFAGSGDPTLANWTLLGSVPNVN
                     TTWSSASPIALGSISNNIYIAFRYTGSGTGGNTTQYNIDDLKITYFQ"
     gene            complement(3422..6022)
                     /locus_tag="PLL59_09735"
     CDS             complement(3422..6022)
                     /locus_tag="PLL59_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY24130.1"
                     /translation="MIQRSLLLFVCLGMTSGVFSKTKYQDQDTVVTTSPSLNDSLYLD
                     KVGENIPTVTLEDVEMNDESGSDQGVSAILSAGRDPFLSAAAFNWGITRFRIRGYDND
                     FYDTYMNGIPTEYIDNGFSSYNLWSGLNDVTRNRENSIGLKLSTFAFGSVGGVYAIDT
                     RASKQRKQLSVTIGTSNRTYDLRGGVTWGSGITKKGWSFAVSVFGRWAKKGYVEGTGM
                     QSISYFASIQKMFKNQSLSLTAFGVPTKQGKVAATTQEAYDLAGSNYYNPNWGYQDGK
                     VRNSRVEYRHQPVIILTHEWKPKENMNLMTAAGYSFGERALSGFDRYNAADPRPDYYR
                     YLPSYIEDTAQRLAAEEYIRENPEVLQVQWNDIYFANKSQGNTTINNVDGVNGNSVTG
                     KRATYVLRDDVQKYQRFNFNTVYNATVKIVDITAGASYQFQQTNQFKRVKDLLGADYY
                     VDVDEFIESDSGQYSDAAQSDLNHPNRVVKVGDKYGYNYNQVIHKAAVWGQTKVNFKK
                     FDISVGTELSYTSQYRFGKFKNGINPDHSQGKSASVNSFNYAVKAGITYKINGRNYLF
                     ANGMYMTRAPFWNKIFISNRTSNEVAADVRSEKVGSVEFGYIFVHPKVKLRATGFYTN
                     FKDGSNSITFYDDFYESLGNYTVTQIDKVHAGVELGLEASIYKGLSATLAASIGKYFY
                     NDRQVGIETVDTDPTFKQVETIYSKGLNVAGSPQQAYALGLNYRSKKFWYVGATVNFF
                     DWMWTDFAPTHRTERAVDAVPYQSEQYNSILQQERLSKKGEWTLDLSGGYSWRLKSTF
                     KNMNGKNASKYYLVLNAGISNVTNNKTIVVGAREQLRFDYEEKDASKFPSRYSYAYGI
                     NFFLNLTFRM"
     gene            6157..7209
                     /locus_tag="PLL59_09740"
     CDS             6157..7209
                     /locus_tag="PLL59_09740"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HMY24131.1"
                     /translation="MKKMTPFLLLISLFISSQSFGQEKQYKIIVAGFYNLENFFDTID
                     DPKTFDNDFTPKGDMHYTADIYFDKIKRLEEVVSQIGTDISPDGLAFWGTAEIENKEV
                     VEALVSQPKLKSRNYQVVHYNGGDPRSIDCGFVYNPKYFKLLKSHSIPIDLSKAVKDG
                     KPTRDVLWVTGILNATDTMDIFIGHWPSRRGGEELTAPMRNYVAKTIKQHTDSIIGLN
                     PNAKVIVMGDLNDNPTDESLIKILQSVDKKEKIKPLGFYNPWISYYNKGIGTLAHNDS
                     WGLFDQIILSKGFLDTKQSGYFYYKANIFNKDFMIQKTGKYKGYPKRTWDFNIYNSGY
                     SDHFPTYITLLKEIKE"
     gene            complement(7212..7925)
                     /locus_tag="PLL59_09745"
     CDS             complement(7212..7925)
                     /locus_tag="PLL59_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMY24132.1"
                     /translation="MSAHIIIAGFTIGILGSFHCIGMCGPIALALPVADATKWKRVFF
                     ILLYNVGRVFAYAIIGAIFGIVGNQFFMGGYQQFLSIFLGVAILLLIFFSKYIAIKSK
                     LFSKYSQSIKQILGKLLRSDKQFYTYLLIGFANGFLPCGLVYVAVAGAVATGSIMESA
                     LFMASFGLGTFPIMVAVTALGKYISLQWRNQIRKAVPILVAFMAILLILRGLNLGIPY
                     ISPIMKATETGTESCCHKE"
     gene            complement(7928..8365)
                     /locus_tag="PLL59_09750"
     CDS             complement(7928..8365)
                     /locus_tag="PLL59_09750"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HMY24133.1"
                     /translation="MSWGKGIAVFIVFFCFVVIAMVYVAMQQTNEMQDKNYYAKELLY
                     QNVIDGKNNLAASPNQLVFRENADSFIVQFPSTLVTDISENSIYLLCPSDESKDYVAA
                     FQLNDKQEHVILKKQLAKVYYTIKISWKNAGKPYYFERSQFIN"
     gene            complement(8391..>9595)
                     /gene="ccoG"
                     /locus_tag="PLL59_09755"
     CDS             complement(8391..>9595)
                     /gene="ccoG"
                     /locus_tag="PLL59_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HMY24134.1"
                     /translation="FNVLEGKFILFSKIFWPQDFHIFAIAMIAFIIFIALFTVIYGRL
                     FCGWVCPQTVFMEFVFRPIEWLIEGNPASQKLLNNGPWSGSKIFKKSLKHFIYLLISF
                     IVANTFLAYIIGMDSLVKIIKEPLSAHLSLFLGLIFFTLTFYGVFAYIREIVCTTICP
                     YGRLQGVMYDKDTMLVAYDYVRGEQRGKFKKNEQRTLGDCIDCHQCVHVCPTGIDIRN
                     GTQLDCVGCTACIDACDFMMEKVGLPKGLIRYASENGIAEGKKMTFTPRIKAYTAVLV
                     GLLVILAVLIATRKEIDTQIARTGGQLYQELPNDRMSNLYNAKIINKTNQEIPVELKV
                     ENLKADIQLVGKPTILLPKEKVLTETFFIILHKNDVKSRTTKLKIGVYRNGEKIQTIN
                     TNFLGPFM"
BASE COUNT         3346 a         1704 c         1548 g         2997 t
ORIGIN      
        1 attagaaaca gctaacaacg taattactga aacgacaagt gagtcttctt caatcaatac
       61 tattgaacaa actacttcaa cagcaaacat tgaagaaaaa atagaaaaca acattgaaat
      121 agaaaatgta gctgaagtaa gtaatacgaa caccgttgaa gttaacgata tcaatgaaga
      181 aaaaacagat gagacttcgc ttattataag cgcagaagaa acagtagaaa cagaagaaaa
      241 ggaacctata aaagacatta taatcgttga cactgctgct cgtacaataa atactcaaag
      301 catagaggta acagatgctc cgaatgaaca agatatttct gttgacaata caagtgttga
      361 agaagtaatg gaaatcaaag ttgtagatgc gaaaactatt gaaaaaacaa atgaagaaga
      421 agacaataca gcagaagata caaaaaatat aattagcgaa ataagcaaca atattgaaat
      481 agaatcaact gaaaaaattg cagctgttaa tactgaacag gcaatttcga ttactcagct
      541 caacaacttt gatgatgaac atgaaacagc attatatcct gagcctttgc ttgtaaaagt
      601 tgaacctgtt tttgctgtca ctcaaaaaga agaagataaa gaaacagtaa tttttgaaaa
      661 caaaactaca aataacaaag ttgacgaaac aaactacaag gaaacatctt cgactacagc
      721 tgaaaacata aacacaacac caaacataga gatagccgtt gtagaaaaaa cagaatcaaa
      781 tattgaagaa aaaattacac ttgaagaaaa taatgaaaca ataattgaac agctggaaaa
      841 acaatcaagt gtattagaaa gcgaatcagc attgtttatt cctgaagtag aagaagatct
      901 agtagaagaa catgtgccat tggaaattgt gacagacaaa gaaaatattg taacgtcacc
      961 aatacatgaa ccaagcattg acgacgaaat tattcctaaa ataatagatg aagaagtcaa
     1021 aagcgaacca catacttttg ttgaatggtt aaaaattcta gacggaaatt tacaaataca
     1081 aactactgaa tctgttaaag aagcggaaca ttggattgaa attcctcgct acgaagtaga
     1141 acaaacgctt gcacataaaa aagagattgc caaagaagaa caaaaactat tcgaacccaa
     1201 ttttgaagaa ggtgaagtgg acttatttaa cgaaattgat gaagaagtaa gcaaggtagc
     1261 caccgagtcc gtcgtgttta aacaagacat gatgaccgaa acattagcaa aaatttacca
     1321 taaacaagga aaattggaca aagctttaga gatttataac acacttcgct tgaaaattcc
     1381 cgaaaaaagt gcttactttg catctttaat tgaaaaaata gaaaaagaaa aataacatgt
     1441 ttacattttt aaccatacta attatcattg taagtttatt tattactttg attgttttaa
     1501 ttcaaaatcc taaaggcggt ggcttgagcg gtggcatcgg cagtgcagct tctaatgtaa
     1561 tcggtgtgca aagtgctggc gatgtaatgg aaaaaacaac ttggggaagc gcaatcgttt
     1621 tattggcatt atgcgtaatg acagcgtttg tagtaccaaa agaaggcgct gctgacaaag
     1681 taaaaacatt aactgaaggt gctgcaaaat ctgctccatc agtgccaaca acgccgacta
     1741 caccagctgc acctcaaaac aatcaacctg ctaataatcc attagctcca aaataattga
     1801 gaataaaata ttctactcaa aaaactcctt ttaataggga gttttttttt gtcgatacat
     1861 attcctttta cacatttcgc tttagtataa tgtaaaacat tgcttattta ttttctataa
     1921 tgcaatgcga tttctatatc attttttaca atcgaagtat taaattaaag cttgtagtaa
     1981 caattcaatt tatctcaact attataaata tttcaaataa aaaatgcctg ctcttgcagg
     2041 cattttttat ttaattatga tattcaatta ttggaaataa gtgattttta aatcgtcaat
     2101 attatattgc gttgtattgc cacctgtgcc acttccagta tatctgaatg caatgtaaat
     2161 gttattacta atgctaccta aagcaatcgg cgatgctgaa gaccatgtag tatttacatt
     2221 tggaacactt cctaaaagcg tccagttcgc taatgttgga tcgccactac ctgcaaagtc
     2281 aattgaatat aaaatctcca atgtagtgga tccagcaggt gaaaaaacgt ttgttctaaa
     2341 gtttaatgtt tcattcgtag aactgcttaa atcaattaat ggcgtaatca accaaccaat
     2401 attagatgct tcgccagaat tataggcact aatacgagca tttttatttg atgagcctgt
     2461 tgcataccaa tatttagaac caacagtact gaagtttgtc caattcggca atgacaagtt
     2521 gcctgacgta gaagcactat taaaatcctc agagaacaat acaacttcta cagggcaagc
     2581 tgctgttgca ggaataagct cagcggcatc tcttatggtt aattgttttg ttgagccata
     2641 aacagaataa acgccaatta atttaatatt tccgattggt gtattttggt tagcaaattt
     2701 tgcaaagtta cttgtacgca cttctatatt gtcgccaaag caatctgtag ctatacggct
     2761 caaggattgt tgtgttataa tatttgccca aggtttatct tggtcagaag cttggaatga
     2821 agcaggatct atttcaatta aacgagcttg aaatgctttc gttaattccg ataatttatt
     2881 tattttaata ggtgtaaccg catttccact tggtcctttt actatatatt ttgtaattaa
     2941 tgaagaagga atgctaccta cagatggctg tccatcagag ttatcaataa agccacccaa
     3001 ctgcaaaaat ttattatatt ctcctaatac taatccttta catttaatgt aaatttttct
     3061 tcctactgga aagtcgttgt acaaaccgct tctgtcaatc aatactggaa taccagccgt
     3121 accatcgtcg atgatgatgg ttttataata gttgccactt ttatcatcag ccaccacaat
     3181 acctgaaatt gtgatgtcat ctgtaatcgt gataggtaaa ccactagtaa ataatgcttt
     3241 taaatcagca attgaagatg ttggtaaatt tggatcaacc gtattaattg gtggttcatc
     3301 aatcttatct ttacgacaag aagtaacaga agataatact acaatcatgg tgagcatcat
     3361 cattagttta ttcaatttag ttatcatata attctgttta atttttattt tgatttgatt
     3421 attacattct aaaagtaagg tttaagaaga agttgatacc ataagcataa gaatatctac
     3481 taggaaattt cgaagcatct ttttcttcat aatcgaaacg caattgttca cgagcaccaa
     3541 caacgattgt tttattattg gtaacattgc taattccagc atttaatact aaataatatt
     3601 tacttgcatt tttaccattc atgtttttga atgtcgattt taatctccaa gaataaccac
     3661 cagacaagtc taaagtccac tctccttttt tgcttaaacg ctcttgttgt aaaatgctat
     3721 tatactgctc tgattgataa ggcacggcat caacagcacg ctccgtacga tgcgtaggtg
     3781 caaagtctgt ccacatccaa tcaaagaaat taacagtagc tcctacgtac cagaactttt
     3841 tagaacgata gttcaaacct aaagcatatg cttgttgtgg cgaaccagca acatttaatc
     3901 ctttactata gattgtttct acttgtttaa atgttggatc agtatctact gtttcgatac
     3961 caacttgtct gtcgttatag aaatatttgc caatagaagc tgctaaagta gcagagagtc
     4021 ctttatatat agaagcttca aggccaagct ccacacctgc gtgtacttta tcgatttgtg
     4081 ttactgtgta atttcctaaa ctctcataga aatcatcgta gaatgtaata gaatttgagc
     4141 catctttaaa gttggtataa aaaccagttg ctctcaattt cacttttgga tgaacgaaga
     4201 tataaccgaa ttcgacagag cctacttttt cacttctcac atcagcagcc acttcgttgc
     4261 ttgttctgtt agagataaaa atcttgttcc aaaatggcgc tctagtcata tacataccat
     4321 ttgcaaacaa ataatttcta ccatttattt tataagtaat accagctttt acagcatagt
     4381 taaaactatt tacgcttgca gattttcctt gtgaatggtc aggattgata ccatttttaa
     4441 atttaccaaa acgatattgt gaggtatatg ataattccgt accaacagaa atatcaaact
     4501 ttttaaaatt gacttttgtt tgtccccaaa ctgctgcttt atgtatcact tgattataat
     4561 tataaccata tttatcacct actttaacta ctctattcgg atgattcaag tcagattgtg
     4621 ctgcatcgga atattgacca gaatcacttt cgataaactc atcaacatct acataataat
     4681 cggcgcctaa taaatctttt acgcgtttaa attgattagt ttgttgaaac tgataagaag
     4741 cacctgctgt aatatctaca atttttactg tcgcattata gactgtatta aaattgaagc
     4801 gttgatattt ttgcacatca tcacgcaaaa cataggttgc tcttttacca gtaacactat
     4861 tgccatttac gccatcaaca ttattgatag ttgtattacc ttgtgattta tttgcgaaat
     4921 aaatgtcatt ccattgtact tgcaatactt caggattttc tctgatgtat tcttcagcag
     4981 ccaagcgttg tgcagtatct tcgatgtagc ttggtaaata acgataataa tctggacgtg
     5041 gatctgctgc gttgtatcta tcaaaaccag acaatgctct ttcgccaaag ctatagcctg
     5101 cggcggtcat taaattcata ttttcttttg gtttccattc gtgtgttaat ataatcacag
     5161 gttgatgtct atattcaact ctcgagttac gcacctttcc atcttgataa ccccagtttg
     5221 gattgtaata atttgaacca gctaaatcgt atgcttcttg tgtagttgca gcaacttttc
     5281 cttgttttgt aggcacgcca aatgctgtca gcgataaaga ttggttttta aacatttttt
     5341 gtatagacgc gaaataagaa atactctgca tgccagttcc ttcaacatat cctttttttg
     5401 cccatcttcc aaaaactgaa acagcaaaac tccagccttt tttagtgata cctgagcccc
     5461 aagttacgcc acctcttaag tcatacgttc tattagaagt accaatggta acactaagct
     5521 gtttacgttg ttttgatgct cttgtatcga tagcatatac gccaccaaca gaaccaaaag
     5581 caaatgttga taatttcaat cctattgaat tttctctatt acgtgtaacg tcgtttaagc
     5641 cagaccatag attgtatgat gaaaaaccat tgtctatgta ttccgtagga ataccattca
     5701 tatacgtatc ataaaaatca ttatcataac cacgaattcg gaaacgcgtg attccccaat
     5761 taaatgcagc tgctgacaaa aaaggatcgc ggcctgcact taaaatagca gacacacctt
     5821 gatcggagcc agattcatca ttcatttcta catcttctaa agtaacagta ggaatgtttt
     5881 cgcctacttt atctaaatat agtgaatcat ttaacgaagg tgaagttgtg acaactgtat
     5941 cttggtcttg atattttgtt ttagaaaaaa caccagatgt catacccaga catacaaata
     6001 aaagcaagga gcgttgaatc ataaaaactg gtattaaaat tgattgttga attaaaaata
     6061 gcttacaaag gtaaagataa atcttcaatt gtatattatg aaacaattca tattttccac
     6121 aaataacatt cacaaatttt tgattaatat tgcactatga aaaaaatgac accgttttta
     6181 ctgttaattt ccttatttat aagtagccaa agttttggac aagaaaagca atacaaaatt
     6241 attgtagcgg gcttttacaa tttggaaaat ttctttgaca caatagacga tccaaaaaca
     6301 tttgacaatg actttacacc aaaaggtgac atgcattata ctgcagatat ctattttgac
     6361 aaaattaaaa gattggaaga agttgtttca caaataggca cagatatttc accagatgga
     6421 ttagcatttt ggggaaccgc agaaattgaa aataaagaag ttgtagaagc attggtgagc
     6481 caacctaaat taaaaagtcg taattatcaa gtggtacact acaatggtgg cgaccctaga
     6541 agtattgact gtggattcgt gtataatcca aaatatttca aactattaaa aagccattct
     6601 attccgattg atttatcgaa ggctgttaaa gacgggaaac cgacgcgcga tgtgctttgg
     6661 gtgactggca tcttaaatgc cactgacacg atggatattt ttataggtca ttggccatcg
     6721 cgccgtggtg gtgaagaatt aacagcacca atgcgtaatt atgtagcaaa aacaattaaa
     6781 caacatacag attccattat tggattgaat ccaaatgcga aagtgattgt tatgggcgac
     6841 ttgaatgaca atcctactga tgaaagttta attaaaatac ttcaaagtgt tgacaaaaaa
     6901 gaaaaaataa aaccgcttgg tttttataat ccttggataa gctattacaa taaaggaatt
     6961 ggcacattag cacataatga ttcctggggt ttatttgacc aaatcatact ttctaaagga
     7021 tttttggaca ctaaacaatc tggatatttt tattataaag ccaatatttt taataaggat
     7081 tttatgattc aaaaaactgg aaaatataaa ggctatccga agcgtacttg ggattttaat
     7141 atctataact caggttatag cgaccatttt cctacctata ttactttgtt gaaagagata
     7201 aaagaataag tttattcttt atggcaacag ctttctgtgc cagtttctgt ggctttcatt
     7261 atagggctga tgtatggaat acctaagttt aaaccacgaa gtatcaataa tattgccatg
     7321 aatgctacta aaatgggaac ggcttttcga atttggttgc gccattgtaa ggaaatatat
     7381 tttcctaatg cggtcactgc aaccataatt ggaaaagtac ctaaaccgaa cgatgccata
     7441 aagagagcac tttccatgat gctgcctgta gctacagcac ctgcgacagc gacataaact
     7501 agaccgcaag gtaaaaaacc atttgcaaac cctattaata gataagtata aaattgctta
     7561 tcgcttcgta ataatttgcc aagtatttgt tttatgcttt gtgaatattt cgaaaataat
     7621 tttgacttaa tagcaatgta tttagaaaaa aatatcagta ataaaattgc aacacctaag
     7681 aaaatagata agaattgttg gtagccaccc ataaaaaatt gattacctac aataccaaat
     7741 attgcaccta ttattgcata cgcaaataca cgacctacat tatacaataa gataaaaaaa
     7801 acacgcttcc actttgtggc atctgcgact ggcaatgcga gtgcgattgg tccacacata
     7861 ccaatacaat ggaagctacc taaaattcca attgtaaaac ctgctatgat gatatgtgca
     7921 gacatatcta atttataaat tgagaacgct caaaataata aggtttacca gcgtttttcc
     7981 aagaaatttt tatcgtgtaa tacacttttg ctaattgctt ttttaggatt acatgctcct
     8041 gcttatcatt tagctggaaa gcagcaacat aatctttgct ttcatctgat ggacataata
     8101 gataaataga attttcagaa atatctgtca ctaaagtgct aggaaattgc acaataaagc
     8161 tatcggcatt ttcacgaaaa actaattgat taggcgaggc tgctaaatta tttttaccat
     8221 caattacatt ttgataaagc aattctttag cgtaataatt tttatcctgc atttcgttcg
     8281 tttgttgcat ggcaacatat accattgcta ttacaacaaa acaaaagaac acgataaaaa
     8341 cggctatacc ttttccccaa ctcatcatca attaattaaa aatttaaaaa ctacataaac
     8401 ggccctaaga aatttgtgtt gatagtttgg attttttcgc catttctata cacaccaatt
     8461 ttcaattttg tcgttctgct tttcacatca tttttatgca aaataataaa aaatgtttcc
     8521 gttaaaactt tttctttcgg caacaatatc gtaggctttc ctactaattg tatgtctgct
     8581 ttcagatttt caactttcaa ttcaacagga atttcttgat tcgttttatt tataatttta
     8641 gcattatata aattgctcat tctgtcgtta ggcaattctt ggtataactg accgccagtg
     8701 cgcgcaatct gagtatctat ttctttgcgt gtggcaatca aaacagccag aataactaac
     8761 aaacctacta acacagctgt atatgctttt atacgtggtg tgaaggtcat ttttttacct
     8821 tcggcaatgc cattttccga agcataacga attaaacctt tgggcagtcc aactttttcc
     8881 atcataaaat cacaagcatc gatgcaggca gtacaaccta cacaatctaa ttgtgtgcca
     8941 tttctgatat ctatacctgt tggacatacg tgaacacatt gatgacaatc tatgcaatct
     9001 cctaaagtgc gttgttcatt ttttttgaat ttaccgcgtt gctcgccacg tacataatcg
     9061 taagctacca acattgtatc tttatcgtac attacacctt gcaatctgcc ataagggcaa
     9121 attgtggtac aaacaatttc gcgtatatag gcaaacacac cataaaatgt aagtgtaaaa
     9181 aaaatcaaac ctaagaaaag agacagatgt gctgacaatg gttctttgat gattttcacc
     9241 aagctatcca tgccaataat ataggctaaa aaagtatttg caacgatgaa tgagattagt
     9301 aaataaatga aatgtttgag tgatttttta aaaattttac ttccgctcca aggaccattg
     9361 tttaataatt tttgtgatgc cggattgcct tcaatcaacc attctatcgg acggaaaaca
     9421 aactccataa aaacggtttg cggacaaacc caaccacaaa acaaacgacc ataaataact
     9481 gtaaacaatg ctatgaaaat gataaatgca atcattgcta ttgcgaaaat atggaagtct
     9541 tgcggccaga agatcttgct gaacaggata aacttacctt ccagcacatt gaaca
//
LOCUS       DAOQQV010000100         9467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2702_length_9467_cov_7.239482, whole genome shotgun sequence.
ACCESSION   DAOQQV010000100 DAOQQV010000000
VERSION     DAOQQV010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2702_length_9467_cov_7.239482"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1652
                     /locus_tag="PLL59_09760"
     CDS             <1..1652
                     /locus_tag="PLL59_09760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HMY24135.1"
                     /translation="IFSSLSYREIDTILDNCQFGYYKFLYVSPERLKTELFIERFKRM
                     NVNLIAVDEAHCVSQWGYDFRPSYLEIANIRQYHAHVPMLALTASATIEVQNDIIAKL
                     NFKNNSVFKKTFKRDNIRFVSRHETAKLPKLIEILQKIIGSAIVYVRNRNKTKEVAEY
                     LSKFNIAADFYHAGLSAIERNKKQDDWINNKINVIVCTNAFGMGIDKADVRVVVHLDV
                     PESIEAYYQEAGRAGRDGKTSYAVLLYNSQDIEKLNESLLEKFPAIADVKRVYNALCN
                     HFELAVESGRMQTFKFDINYFSQQFNLSPSLIYNSLKLLEQENYLQLSENDWMPSRAT
                     FIVDQNELYRYEVAHATHTNLIRMMLRTYGGILDHYIKINEARLAKQLNCTENEIKQH
                     LIAMHKNNILVYVCASELPTILFLNERLHDNNLYFNVNYIQQRKKIIQEQIQAIIRLT
                     EETVTCRQVIICTYFGENNIENCGVCDNCIKEKKEREAKHTFELIKQDILNKTKNNWV
                     KSEEILPSEAHFSAQQYKDVIRFLLDENILQLNDKNELKSV"
     gene            1645..2733
                     /locus_tag="PLL59_09765"
     CDS             1645..2733
                     /locus_tag="PLL59_09765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMY24136.1"
                     /translation="MSKKIICTVTNDLVYDQRMQRICTSLQGNSFSVELVGRTLERSV
                     TLPRSTFKQTRLTCYFNKGFLFYAEYNIRLFIFLLFNSFDIVCGCDLDTLPAAVLAAK
                     IKRKKVVYDAHEYFPESPELIGKPKKQAFWLLLEKILIPRVDAMYTVTASIAKIFVKK
                     YNVDAKLIRNLPMKDKVKLNNNKQAILLYQGAVNIGRGINEMLLAMLKIENAAFWIAG
                     DGDEMQHVQHSVKELQLENKVKLLGKKLPTDLQKITQQSFIGINLLENRGLSYYYSLG
                     NKTFDYIQAGVPQIMIGFPEYIALNKQYNIGVLVDEMTVEHIVNAIQILLNDKVLYRK
                     LQENCMQARKELCWQNEEMKLLTIYNSL"
     gene            2736..3899
                     /locus_tag="PLL59_09770"
     CDS             2736..3899
                     /locus_tag="PLL59_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24137.1"
                     /translation="MALSITPNFALNIVCYDVPFPADYGGAMEEFYKIKSLHQLGAKI
                     YLHCFVYGNRQASQTLEKYCVKVYYYQRQRKLKDAFKKTPFIVKTRMDNELLRNLQSN
                     NFPILFDATHTTGFINEPSLKSRVKVVRLHNIEWIYYHILFHSAFSLYEKIFYFLEYK
                     KLKKHDKYLTFADGLSCLSDTDYAYYSDKFPKKNIQYIPVFHENSEVTCKEGKGKYLL
                     YHGNLSLIDNCEVIINVLSNELKNCHYPIIIAGKNPSPTLIDFVKNKSNIQLQPNPSQ
                     AQLDNLIEDAHICLAIAKNPSGVKLKLINSLFKSRFVLCNSSALNGADVDDLCVNIDE
                     VENVQQMIDDLMQQPFSNDMITFRKQVLLSKYNNIGNAHALLDFMDKITKNEK"
     gene            3889..4389
                     /locus_tag="PLL59_09775"
     CDS             3889..4389
                     /locus_tag="PLL59_09775"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HMY24138.1"
                     /translation="MKNKIFPIALIIVFANVLFFVVFKGKKAPEIPLSKTELFDEQNK
                     IVDWNANKHKATIISYYQSWCGECREELLELEALQQIVGGEQQLKIIMISDEPWNIIQ
                     SVSVKSKKSIQFVQSKNKLRDIGIRRFPTTYFLDENLKVVDAKVEGIHWNTKEIQDRI
                     FMMNKQ"
     gene            complement(4400..5371)
                     /locus_tag="PLL59_09780"
     CDS             complement(4400..5371)
                     /locus_tag="PLL59_09780"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMY24139.1"
                     /translation="MKILITGITGLIGKHVMHVLLKNQLTNIRGHYFSSKNINEYISK
                     NIEMVQADITDKNAIHNITNSCNIVVHTAARVIDFGTKKQFYDAHYEATKFLLEDAIN
                     NKVEHFIYISSVGVASGIDRNKIIPNEATPLVKTGILYDDVKIDTEELVKSICDNNNI
                     YYTIIRPTAVIGIGSVWVNEPLKRMETSLGLKLIDNGKHAACLLDAENLANGIYLCIT
                     KEIARNQIYFFMDDWNISWKKYFTDLANMKGKKLSGSMPYSLAYFIASIAEIIFPLFG
                     KKPPLAKKSAQATGKNRTVSTQKARSELGWTSAKTYEQSMREIKESL"
     gene            complement(5394..7403)
                     /locus_tag="PLL59_09785"
                     /pseudo
     CDS             complement(5394..7403)
                     /locus_tag="PLL59_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            7502..>9467
                     /locus_tag="PLL59_09790"
     CDS             7502..>9467
                     /locus_tag="PLL59_09790"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMY24140.1"
                     /translation="MIKYLSIIFTTCILFLGANVKAAEKNSNIGWCASEAPSMEWENQ
                     IQRIIATQRANNMANGREAQVSYTIPVIVHVCYYTNNSSQNIPANRVQNQITVLNNDF
                     AGIGLNSNTVPAAFAPLIANSNVTFCLAKLDLNNNVLAEPGIDRVNAQQRGWGSPSTS
                     TGWSRDFIDNTIKKATIWDPNYYLNIWVVPYIGGFSSSILGYATFPGMSGLPGMPSGA
                     GNPLNDGFVCRASYFGNTGGGRTSTHEIGHWLGLRHIWGDDSCGTDFVNDTPTAFEPN
                     YGCPSYPHRSCGNTTSGDMFMNFMDYTDDNCMAMFTNDQSARFQVVMQNADFRKNLQY
                     SPVCDTVKQKPVAKFSYLQQVSTLCATSKKIDFTDKSLFAPTSWSWTFEGGTPATSNQ
                     QHPAGITFNTAGIHNVKLVVSNSRGVDSSVFQVNVSIVGNAAMPLLEDFEGPIFPSAG
                     WDLVNRNGNAAINWDRISGYSAFGVGNNCMRFDNTTLDGRFQKDDIMTPKLNLTGVSQ
                     ATLKFDVSHAPFYGQLTQNGPDEHLWDTLEVLITDNCQQTTQRIYRKGDSVLATVMPG
                     QGDEFYPQANQWRTETIVIPASYLNKQNVQIIFRNYGDFGHTIYVDNINVFAQNSTPT
                     ATASFTVSDTTVCVGGSLTFTNTSTASSGSP"
BASE COUNT         3152 a         1492 c         1591 g         3232 t
ORIGIN      
        1 ctatattttc tagtttgtcg tatcgagaaa tagacacgat tttggataat tgtcaatttg
       61 gttactataa atttctgtac gtatcacctg aacgactaaa aacagaactt tttattgagc
      121 gttttaaaag aatgaatgtc aatttaattg cagttgacga agcacattgt gtatcgcagt
      181 ggggctacga ttttaggcca tcatatttgg aaattgcgaa tattcgtcaa taccatgcac
      241 atgtgcccat gttggcgctc acggcatctg ctactataga agtgcaaaac gatatcatag
      301 ccaaattgaa ttttaaaaat aatagcgttt tcaaaaaaac attcaagcgt gataacatca
      361 gatttgtatc gagacatgaa acggcaaaat tgccaaagct catcgaaata cttcaaaaaa
      421 taataggctc agcgattgta tatgttcgga atagaaataa aacaaaagag gtagcagaat
      481 atctttctaa gtttaatatt gctgccgatt tttatcatgc aggattgagc gcaatagagc
      541 ggaataaaaa acaagatgat tggataaata ataaaatcaa tgtaatcgtt tgcacgaatg
      601 catttggaat gggcatcgat aaagccgatg tgcgtgtggt ggtgcactta gatgtgcctg
      661 aaagtattga agcctattat caagaagctg gccgtgccgg cagagacgga aaaacttcgt
      721 atgctgtgct cttgtacaat tcacaagata tagaaaaact aaacgagtct ttgctcgaaa
      781 aatttccagc aatagcagac gttaaacgcg tgtataacgc actttgcaat cattttgaat
      841 tagctgtaga aagtggaaga atgcaaactt ttaagtttga tataaattat ttctctcagc
      901 aatttaattt atcgcctagc ttgatatata atagtttgaa attattagag caagaaaatt
      961 atttgcaact aagtgaaaat gactggatgc catctcgtgc tacctttatc gttgatcaaa
     1021 atgaattgta tcgttatgaa gtcgcacatg caactcacac caacttaata agaatgatgt
     1081 tgcgcacgta tggcggcatt ttagatcatt atattaaaat caatgaagct cgcttagcaa
     1141 aacaattaaa ttgtacagaa aatgaaataa aacagcattt gattgccatg cataaaaata
     1201 atattctcgt gtatgtgtgc gcaagtgagc taccaacaat tttgtttttg aatgaaagat
     1261 tgcacgataa taatttgtat tttaatgtca actatattca gcaacgtaaa aaaataattc
     1321 aggaacaaat tcaggcaatc atacgtttaa cagaagaaac tgtgacgtgc cgacaagtga
     1381 taatttgtac ttattttggt gaaaataata ttgaaaattg tggagtttgt gataattgta
     1441 taaaagaaaa aaaggaacga gaagcaaaac atacttttga gttgattaaa caagatattt
     1501 taaacaaaac aaaaaataat tgggtgaaat cggaagaaat attgcctagt gaagctcatt
     1561 tttctgcaca acaatacaaa gatgtcatta ggtttttgct tgatgaaaac atattacaat
     1621 taaatgataa aaatgaatta aaaagtgtct aaaaaaatta tttgcaccgt aacgaatgat
     1681 ttggtttatg accaaagaat gcaacgtatt tgtacgtcgt tgcaaggcaa tagtttttca
     1741 gtggagcttg taggtagaac acttgaacgt tccgttacat tgccaagaag cacttttaaa
     1801 caaacgcgat taacttgcta ttttaataaa ggctttttgt tttatgcaga atataatatt
     1861 cgattgttca tttttttgct atttaattct tttgatatag tttgtggttg tgatttagat
     1921 actttgcccg cagcagtgct agcggcgaaa ataaaaagaa aaaaagtagt gtatgatgca
     1981 catgaatatt ttccagaaag ccctgaatta ataggcaaac ccaagaaaca agccttctgg
     2041 ttgctattag aaaaaatatt gattcctcgt gttgatgcta tgtacacagt aacagcatca
     2101 attgcaaaaa ttttcgtgaa gaaatacaat gtagatgcaa aattaattcg caacttgcca
     2161 atgaaagata aggtgaaatt gaacaataat aaacaagcta tattgttgta tcaaggagca
     2221 gtaaatattg gtagaggcat taatgaaatg ctattggcaa tgctaaaaat agagaacgca
     2281 gctttttgga ttgctggtga tggcgatgaa atgcaacatg tgcaacattc tgtaaaagaa
     2341 ttacaattag aaaataaagt aaaattgctt ggcaaaaaat tgcctacaga tttacaaaaa
     2401 atcacacagc aatctttcat aggaataaat ttattggaaa atagaggctt aagttattat
     2461 tattcattgg gtaataaaac gtttgattat attcaagctg gtgtgcctca aattatgatt
     2521 ggttttccag aatatattgc cttgaataag caatataata ttggtgtgct tgtcgatgaa
     2581 atgactgtag agcatatcgt taatgcgatt caaatattgt taaatgataa agtgctttat
     2641 agaaaattac aagaaaattg tatgcaggct cgtaaagaat tatgctggca aaacgaagaa
     2701 atgaaattac ttacaattta taattcttta taaaaatggc tttatcaata acaccaaatt
     2761 ttgcattaaa tattgtatgc tacgatgtgc cttttcccgc tgattatggc ggcgcaatgg
     2821 aagagtttta taaaattaaa tcgctacacc aacttggtgc gaaaatttat ttgcactgtt
     2881 ttgtttatgg caatagacaa gcttctcaaa ccttagaaaa atactgtgtc aaagtatatt
     2941 attatcagcg tcaaagaaag ttgaaagatg catttaaaaa aacacctttt atcgtaaaaa
     3001 cgcgaatgga taatgaatta ttaaggaact tacaaagtaa taattttcca attctattcg
     3061 acgctacaca tacgacaggt tttatcaatg agccgtcatt aaaaagtaga gttaaagttg
     3121 tgcgcttgca caacattgaa tggatttatt atcatatttt atttcattcc gctttttcac
     3181 tttatgaaaa aatcttctat tttttagaat ataaaaagtt gaaaaaacat gacaaatact
     3241 taacttttgc tgatggtttg tcgtgtttgt ctgatacaga ctatgcttat tatagcgata
     3301 aatttccgaa aaagaatatt caatatattc ctgtttttca tgagaattcc gaagtgactt
     3361 gcaaagaagg aaaaggaaaa tatttacttt atcatggcaa tttatcactg atagataatt
     3421 gtgaagtgat aataaatgtg ctgtctaatg aattaaaaaa ttgccattat ccaattatta
     3481 ttgcaggcaa aaatccttcg ccaacgctga tagattttgt aaaaaataaa tctaatattc
     3541 aattacagcc aaatccatct caggcacaat tagataactt aattgaagat gctcatattt
     3601 gtttggccat tgcaaaaaat ccatcaggcg taaagctaaa attgataaat tcattgttta
     3661 agtcacgatt cgtgctatgt aattcaagtg cattaaatgg tgccgatgtt gatgatttat
     3721 gcgttaatat agatgaagtt gaaaatgtac agcaaatgat cgatgatttg atgcaacagc
     3781 ctttttcaaa tgatatgatt acatttcgaa agcaagtgct cttgtccaaa tataacaaca
     3841 ttggcaatgc acacgcattg cttgatttta tggataaaat tactaaaaat gaaaaataaa
     3901 atatttccga ttgccttgat tattgtattt gcaaatgtac ttttcttcgt agtttttaaa
     3961 ggtaagaaag cacctgagat tccattgtca aaaactgaat tatttgacga acaaaataaa
     4021 attgtagatt ggaatgctaa taaacacaag gctactataa taagttatta tcagtcatgg
     4081 tgtggcgaat gcagagaaga attgctagaa ttagaagcat tgcagcaaat agtaggaggc
     4141 gagcagcaat taaaaataat tatgatttct gatgagccat ggaatattat ccaaagcgta
     4201 agtgtcaaat ctaaaaagtc gattcaattt gtgcaatcaa aaaataaact acgtgatata
     4261 ggtattcgac gttttcctac tacttatttt ttggatgaaa atttgaaagt agtagatgcg
     4321 aaggtcgaag gaattcactg gaatacaaaa gaaattcaag atagaatatt catgatgaac
     4381 aagcagtaaa atcagtcgtt tacaagcttt ctttaatttc tcgcatagat tgttcgtatg
     4441 tttttgcaga tgtccaacct aattcgcttc ttgctttttg tgttgaaact gttctgtttt
     4501 ttcctgttgc ttgtgccgat ttttttgcaa gaggtggctt tttaccaaat aatggaaaaa
     4561 taatttcagc aatactcgct ataaaatatg ctaaagaata tggcatcgag ccacttagtt
     4621 ttttaccttt catattagcc aagtcagtga aatatttttt ccaagaaata ttccaatcgt
     4681 ccatgaaaaa atatatttgg ttcctagcta tttctttagt gatacataaa tatattccat
     4741 ttgctaaatt ttcggcatcg agcaaacaag cagcatgttt gccattgtcg atgagcttta
     4801 aacctaatga agtttccatt ctttttaagg gttcatttac ccaaacgcta cctatgccaa
     4861 ttacagctgt tggccgtatg atggtgtaat atatgttgtt attatcacag atgcttttta
     4921 ccaattcctc cgtgtctatt ttgacatcat catacagaat gccagttttt actaatggtg
     4981 tcgcttcatt aggtattatt ttatttctgt caatgccact ggcaacacca acagaactga
     5041 tgtaaataaa gtgttctact ttattgttga tggcatcttc taacaagaat tttgtagctt
     5101 cataatgtgc atcataaaat tgttttttgg taccaaagtc gattacgcgt gcagctgtgt
     5161 gcaccacaat attacatgaa tttgtaatgt tatgtatggc atttttgtca gtaatatctg
     5221 cttgcaccat ttcgatattt ttagaaatat attcattaat attttttgat gaaaaatagt
     5281 gtccacgaat atttgttagt tgatttttaa gtaaaacatg cattacatgt ttacctatta
     5341 atcctgtaat tcctgtaatt aatattttca tttttatgat attttatatc gtcttaattt
     5401 cttggaaata gtagtgttaa tgcatcttct gtgttttcaa ttccttgatg acagcttttg
     5461 tttatgcata cataaattag cgtttttccc tctttatatc tttctttaca aagtgcaaat
     5521 tcgcttttat gctcacttcc agaaaataat acatttggca aatagttttt ttgtaattgc
     5581 ttcatttttt ctttggcatc tttacctacg atgactactt cagtattgct atgtaaatgc
     5641 aagtctaaga cgatagccca atttgagaaa tatctgccat cttttataat ttctgctaac
     5701 acattattca acattgtcgc actcattttt tcatattctg aattgtcaaa atatttgcta
     5761 agtaagaata agttctttgc cattgttgag tttccagacg gtatcacatt gtcgcttgtt
     5821 tctgtacttc ttacaattaa ttgtttgtcc atatcagaag taagataaaa taaaccattg
     5881 ctactattgt aaaaatgttt aatcgtatat tccaaaaagt tttttgcctc atctaaatat
     5941 ttttctatta aagtgacttg atgtaatgat attaatgcgt ctataaaaaa tgcataatct
     6001 tgcaaaaaag cattaatact tgattttcca tttttgtaat ttctgtctaa tctatagttt
     6061 tcttttttac aattactaag aataaaattt gtaatttctg ttgccttttt taaatacata
     6121 tcatcaccaa aagctttgta agcatcacac aatcctttta taaaaagcgc attccattct
     6181 gttaatattt tatcatcagt agctggcttc attttattgt ttcttgcatg taataaaatc
     6241 caatgtaaga cagaaagttt ttgatggaat tgttcattgc ttaatttgta tttctctata
     6301 aaatattcat gttctctagt gcgaaataga atattcaaat tattttcaaa atttccattt
     6361 tcttcaatat tatatacatc acaaaagaaa cgaaaatcat ctttcaataa agtttccaat
     6421 tcgccgtaag tccagcaata atattttcct tctattcctt cactgtcggc atctaatgct
     6481 gaatatatgc cgccactttt atcagtcatt tctctttcta tccaataaaa tatttgctca
     6541 catacacttt tgtacatagg tcgtttagtc agttgatatg cttgcgcata taagcttatt
     6601 atttgcccat tatcatacag catcttctca aagtgaggca ctttccataa cgcatctacg
     6661 ctgtagcgcg caaagccacc tccaattata tcatgtatgc caccaaaagc aactttcttt
     6721 aatgtggttt taatgaagtc tgcaacgtca ttattttttg tttgataata ataacgcata
     6781 agatattcat aattatttgg catcaaaaat ttgggagctc ctttgctgcc gccatcttct
     6841 ctgtctattt tttccaacca attttcccat attgtatcga gtatttctac attaattttc
     6901 tgtgtttttt gtaatggtat attttcaatt tgatgtaaac cttcacttat tttttttgct
     6961 tgaatttcaa gtttttgtct gtcttcgtta tatagtttta taaaatagtc taatacttga
     7021 atccattgct gttttggaaa ataagtgcct gcaaaaactg gccttccatc gggcatggcg
     7081 atgatattta aaggccagcc accttgtccc gtcgttatca tggctgcatt catgtatata
     7141 ttgtcaatgt ccggtcgctc ttctcgatct acttttattg atataaaatg aatattcatg
     7201 atttctgcta cctcaaaatc ttcaaaactc tcgtgttcca ttacatgaca ctaatgacag
     7261 gcagcataac ctattgaaat aatcaacatt ttattttcag cttttgcctt gtctaatgca
     7321 acgtcactcc acgaatacca atctattgga ttgtttcggt gttgtagcaa ataaggtgaa
     7381 ctttcatatt gtaaatgatt catattcttt ttattgcatt tttcttcaat attaataatt
     7441 aagtagattc tatgataaaa gtgaagaatc ttttattttt gtaacgaaaa ttaactaatt
     7501 tatgataaaa tatttaagca tcatttttac aacatgcatt ttgtttcttg gagctaatgt
     7561 aaaagcagct gaaaaaaaca gtaatatagg ctggtgtgcc tccgaagcac ctagcatgga
     7621 gtgggaaaac caaattcaac gtattattgc tacgcaaaga gcaaataata tggcaaatgg
     7681 ccgagaggca caagtgagtt ataccattcc tgtgattgtt cacgtttgtt attacacaaa
     7741 taattcatct caaaatattc cagcaaatag agttcaaaat caaatcacag tacttaataa
     7801 tgactttgct ggtattggat taaacagcaa tactgttcct gctgcatttg ctccattaat
     7861 tgctaattca aatgttacat tttgtttggc aaaattagac ttaaataata atgtcttggc
     7921 agagcccggt attgaccgtg ttaatgcaca acaaagaggc tggggaagtc catctacctc
     7981 tactggttgg tccagagatt ttattgataa tacaataaaa aaagctacaa tctgggatcc
     8041 taattattat ttaaatattt gggtcgttcc atatattgga ggctttagta gttctatttt
     8101 aggatatgct acttttccag gaatgtctgg tttaccaggc atgccatcag gcgctggtaa
     8161 tccattaaac gatggattcg tatgtcgtgc atcttatttt ggaaatacag gtggcggtag
     8221 aacttctaca cacgaaattg gacattggct aggcttaaga catatttggg gcgacgatag
     8281 ctgtggtact gactttgtca acgatacacc tactgccttt gagccaaatt atggctgccc
     8341 ttcatatcca cacagatctt gtggcaatac aacaagtgga gatatgttta tgaattttat
     8401 ggattataca gatgataact gtatggctat gtttacaaat gatcagtcag cacgttttca
     8461 agtagtaatg caaaatgctg attttagaaa aaatttacag tactcaccag tttgcgatac
     8521 tgtaaagcaa aaaccagtag ctaaattttc ttacctacaa caagtttcaa cattatgcgc
     8581 tacatctaaa aaaattgact ttacagataa atcacttttc gctccaactt cttggagttg
     8641 gacttttgaa ggcggtaccc ctgcaacttc taatcaacag cacccagctg gtatcacatt
     8701 caatacagca ggtattcaca atgtaaaatt agttgtgtct aatagtagag gtgttgattc
     8761 ttctgttttt caagttaatg tttcgattgt gggaaatgct gcaatgccat tgttagaaga
     8821 ttttgaagga ccaatctttc catctgctgg ctgggattta gtaaatagaa atggaaatgc
     8881 tgccatcaat tgggatagaa ttagcggtta tagtgcattt ggcgttggaa ataattgtat
     8941 gcgttttgac aatactacat tagatggtag attccaaaaa gatgatatca tgacacctaa
     9001 attaaattta acaggtgttt ctcaagcaac tttaaaattt gatgtttctc atgctccatt
     9061 ttatggtcaa ttgacgcaaa acggtcctga cgaacattta tgggatactt tagaagtttt
     9121 aattactgat aattgtcagc aaaccacaca acgaatttat agaaaaggag atagtgtttt
     9181 agctacagtt atgcctggtc aaggtgatga attttatcct caagcaaacc aatggagaac
     9241 agaaacaata gtaattccag caagctattt gaataaacaa aatgtacaaa ttatttttag
     9301 aaattatggt gactttggac atacaattta tgttgataac atcaatgttt ttgcacaaaa
     9361 ttccacacca acagcgacag cgagttttac ggtcagcgac actacggtat gtgtaggagg
     9421 tagtttaaca tttacgaata cctcaacggc aagcagtggc agtccgg
//
LOCUS       DAOQQV010000101         9384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2737_length_9384_cov_7.229821, whole genome shotgun sequence.
ACCESSION   DAOQQV010000101 DAOQQV010000000
VERSION     DAOQQV010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2737_length_9384_cov_7.229821"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLL59_09795"
     CDS             <1..382
                     /locus_tag="PLL59_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HMY24141.1"
                     /translation="AIPKYISQQKTMLSLTILSMVLVVLSVITTGKVAIVCFMLLGFS
                     NAVMWPAIWPLAIDGLGKFTKMGSALLIMGIVGGAILPPLYGKISEIINNKQTAYLMM
                     LPCYLYILYYAIVGHNVGREKETK"
     gene            379..1710
                     /locus_tag="PLL59_09800"
     CDS             379..1710
                     /locus_tag="PLL59_09800"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMY24142.1"
                     /translation="MKYLIKNGHVVNEGKIKQKDILIVDEYISKIDTDISDETAIVID
                     ASNQYIIPGIIDDQVHFREPGLTHKATIYSESKAAIAGGVTSFMEQPNTKPAALTQNL
                     LEEKYQIAAQTSLANYTFFMGTSNDNYDEVMRTDFSKIGALKIFMGSSTGNMLVDDEH
                     ILNKIFANIGGIVVTHCEDEQTILQHTEKFKSLYNENIPMNFHPIIRSDEACYLSSKL
                     AIDLAKKHQTRLHIFHVSTAKELDLFSNQQALKDKKITAEVCVHHLYFDERDYAALGS
                     KIKCNPAIKFESDKRALLQALLDDKLDVIATDHAPHTLQEKNNPYLTAPSGLPLVQHS
                     LNMMLDFYHQQKISLEKIIEKMCHAPAECFKIKNRGYLREGYYADIAIVDIHQRWSIS
                     TENILYKCAWSPLENISVYGKINQTFVNGHLVYNKGIFDESKKGHRLLFSV"
     gene            complement(1722..2696)
                     /gene="fbp"
                     /locus_tag="PLL59_09805"
     CDS             complement(1722..2696)
                     /gene="fbp"
                     /locus_tag="PLL59_09805"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006778.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HMY24143.1"
                     /translation="MRLITFDEFIAARQHELPDATGELTGLLRDIGLAAKIVNRDLSR
                     AGLVDAILGDADSINVQGEDVKKLDIIANEVLKKCLQNSGECCGIASEEEEDFVAFDN
                     RKNAKYVVLFDPLDGSSNIDVIAPVGTIFSVFARLSKKGDLCTLDDFLQPGHKQVAAG
                     YVIYGSSTMLVYTTGDGVNGFTLDPTIGEFCLSHSNIKTPSTAKTYSINQGNFNSFSD
                     KVKSFVDYCQDSTNKKAYSLRYIGSMVADFHRNLLKGGIFIYPATKDAPGGKLRLMYE
                     CNPLAFLQEQADGTASNGTQRILDIIPEKLHQRTVVYVGSQSLVEMLL"
     gene            2834..3301
                     /locus_tag="PLL59_09810"
     CDS             2834..3301
                     /locus_tag="PLL59_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY24144.1"
                     /translation="METKILIRKAEPADLTAVYALIKELAIFEKEPNQPSNSLEKFIQ
                     DGTAKQPHFHVIVAEIEAQIVGMAMYYFGYSSWKGAMLYLDDLIVKEAYRKHGIGKKL
                     VDELINIAKEERVSLMKWQVLDWNENAIKFYEKYPIVKDASWIDIKIEKENLQ"
     gene            3301..4554
                     /locus_tag="PLL59_09815"
     CDS             3301..4554
                     /locus_tag="PLL59_09815"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMY24145.1"
                     /translation="MKVFKFGGASVKDAAGIKNVASIIQKYGEKNLVVVVSATGKTTN
                     ALEEVIKAYFNDTDAKAAFDVVKKNHHQICIDLFGNDTHKVFDSLQNIYAEVDWQLEE
                     KRKESYNYVYDQIVSQGELISSVILSAYMNEIGINNTWLDVRDVMKTDDTYREAVVDW
                     NKTQELMNAKVKQMIDGSIAVTQGFIGCTAENCTTTLGREGSDYTAAIFANMLDAEHQ
                     TIWKDVPGVMNGDPRVFEDAVFIDEISFNEAVEMTFYGATVIHPKTIKPLQNKGIPLL
                     VKSFIHPDGKGTVVGGNDKNLPPIRIVKNNQALITLHTKNFSFVEDEVIAEIFSSFFK
                     ANLKLNMMQQGAISFEAVVDNIPEKIAKVKDALQDKFNFVIDENLTILTIRHYTDAAY
                     INKFKNERSTILEQRRPSTYQGLLA"
     gene            complement(4631..8074)
                     /locus_tag="PLL59_09820"
     CDS             complement(4631..8074)
                     /locus_tag="PLL59_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24146.1"
                     /translation="MHVHAQIRTLDFSIYSNQRSKVVYITSDTIQLDTLSIFPNSLQI
                     RNLLNDEQLSSDSVFFDFAKATIVFKNKQRPDSIIVSYRTFPTALAGISQHKNPSLIR
                     KGDSLFYQRFYYQPKAEQTASFLDFGGLNYSGNFARGIQFGSNQDLTLNSTLDLRLSG
                     KIGNGIEITASLTDNNIPLQPDGTTQQLQDFDKVFIQLKKEPHTVIAGDYALKNYDSY
                     FLKFAKQLQGASYVNHLIFKNGMHLQTSTSMAVSRGKFARNVFSGTEGNQGPYRLVGN
                     NNETFLIILAGSEKVYVDGVRLQRGELQDYIMDYNTGEIIFMPRFLITKDSRIVIEFE
                     YADRNYFRTTVHEAIRFSWKKLNIFTQVYAEQDAKSKPISNAISDSNKLFLSTIGNDI
                     SNAYVSGVREVAFDPNRVQYKMKDTTVNAILYDSVFVYETNPDSAKYLLTFSYLGTGK
                     GNYLPTKNANNVRVYEWISPVNGVPQGSYEPLILLVTPKSQLLFTFGADYQIDNKTKV
                     RAEMGFSNYDKNLFSPIGKKENKGYAANVQLQRIDSFKHKIVFTNALNYEFKSSNFQQ
                     VEPYRNVEFSRDWNIRNALNYTEHIANYMTELKDIKHNISASYRFSSFIRQKQYQGFE
                     NRIRFGMDYKQVKIDIDARLLNTQDNMFKSLFFRPQINASYTIAKARGIGFGVGFYQE
                     NNVFKSIATKELTNNSFRNNNLSFQFFMPDSAKWNFMLAYKYRKDSKVKNNAFSDSTT
                     AHTVEFKGGANQLKNQTFNWTFTYRNLLVKDTSIQQQAEQTFLGRTEYGARIKKGVLR
                     LNFIYELGGGRERVRQFTYVDVPSGQGYFKWIDQNDNGIRELNEFVPSQFNDSAQFVR
                     VLTDLNEYVNANSTAYTQNITIQPKAIWFNQSGFKKFMSKLTLQSYMQLRRKTYKSNI
                     AKAFNPFVFKTSDSAIISLNTNIRNSFIFNAGDPIYSFSFTNQMVSDKILLVNGFDTR
                     KRMDNIIEAYYNLSQKFTLNLKFIQSKIALVSDYFQPNNYLIRAYYVEPKFTFNYKTM
                     LRTSVNYGFTHKQNEIQLGGERTLANKLEFELRYNKALKNTLEAKFSFVQVKYNGENG
                     TTKSYTILEGLQPGSNYIWSSTYTRNISQNLELSLTYEGRKTGTAKLINTGRAQLRAV
                     F"
     gene            8201..8770
                     /locus_tag="PLL59_09825"
     CDS             8201..8770
                     /locus_tag="PLL59_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HMY24147.1"
                     /translation="MNTLDLIKNRRSIDPDHFTGAMVEHHIIENMLEAANWAPTHGYT
                     EPWRFIVYEGEKIASFSEFHASLYQKHTNAEQFLQKKYEKLKNRARLCSHVIICVNKR
                     GDKSNIPALEEIAATSAAIQNMLLVATSNQVATFWSTGGMCYHQSFAEELGFTIEDQV
                     LGILYIGKSEQVQYEGKRISEWQAIVTWK"
     gene            8839..9339
                     /locus_tag="PLL59_09830"
     CDS             8839..9339
                     /locus_tag="PLL59_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24148.1"
                     /translation="MSIEIFHFVRFLHVVCASAWFGEVVVINFVLIPALSRYQGVYKK
                     DFLNTIFPKIFNLASVLAGTTAVTGGILLYHFIGVDIVSLTSKGTWGWSILIAGTLGL
                     VLTIFHFFIENNLAKKVGVGDPNLSQDSVEDVHLKLKFIPRAGLVVITTIFLLMLNAT
                     HALISF"
BASE COUNT         3204 a         1527 c         1576 g         3077 t
ORIGIN      
        1 tgccattcca aaatacatat cacaacaaaa aacaatgttg tcattgacta ttttaagcat
       61 ggtattagta gttctttctg ttataactac aggaaaagtt gcaatagttt gttttatgct
      121 tttaggattt tctaatgccg tcatgtggcc agctatttgg cctttggcaa ttgatggttt
      181 aggtaagttt acaaaaatgg gttctgcatt attaattatg ggcattgttg gcggtgctat
      241 cctgccacca ttatatggta aaatttctga aataatcaac aataaacaaa ctgcctatct
      301 aatgatgcta ccttgttatt tatatattct atactatgcc attgttggac ataatgtggg
      361 aagagaaaag gaaacaaaat gaaatattta ataaaaaatg gacacgtcgt caacgaaggt
      421 aaaattaaac aaaaagacat tttaattgtt gatgaataca tcagcaaaat tgatactgat
      481 atttccgatg aaacagccat cgtaattgat gcttcgaacc aatatattat tcctggaatt
      541 atagatgacc aagtccattt tagagaacca ggattaacgc acaaggcaac aatctatagt
      601 gaaagtaagg cggcaatagc tggcggtgtt acttcattta tggagcaacc caatacaaaa
      661 cctgcagcgc tgacacaaaa tttattagaa gaaaaatatc agattgcagc gcagacatca
      721 ttggcgaact atactttttt tatgggaaca tctaacgata attacgatga agtaatgcgt
      781 acagattttt ctaaaattgg agcattgaag atatttatgg gctcatcaac aggaaatatg
      841 ctggttgatg atgagcatat tctcaataaa atatttgcaa atattggtgg tatagtggtt
      901 actcattgcg aagatgaaca aactattttg caacacacag aaaaattcaa atctttatac
      961 aatgagaata ttccgatgaa ttttcatccg attattcgta gcgatgaagc ttgttattta
     1021 tcttcaaaac tggctattga cttagcaaag aaacatcaaa cacgtttgca tatttttcat
     1081 gtttctactg caaaagaatt agacttattt tccaatcaac aagcattaaa agacaaaaag
     1141 ataacagctg aagtctgtgt gcatcattta tattttgatg aacgagacta tgcagcttta
     1201 ggtagtaaaa tcaaatgtaa tcctgccata aaatttgaat cagacaaaag agcattactg
     1261 caagcattat tagatgataa attagatgtc attgctactg atcatgcgcc gcatacatta
     1321 caagaaaaaa ataatcctta cctaacagca ccatctggct tgccattggt tcaacatagt
     1381 ttgaatatga tgttagactt ctaccatcaa caaaaaattt ctctagaaaa aataattgaa
     1441 aaaatgtgcc acgctcctgc tgagtgtttt aaaataaaga atcgcggcta tttgcgtgaa
     1501 ggttactatg cagatattgc catagtagat attcatcaac gctggtctat ttcaacagaa
     1561 aatatcttgt ataaatgcgc ttggagtcct ttggaaaata taagtgttta tggcaaaata
     1621 aatcaaacat ttgtcaatgg acatcttgta tataacaaag gcattttcga cgaaagtaaa
     1681 aaaggacaca gattattatt ttctgtgtaa taaaaagctt cttataggag catctctacc
     1741 aatgattgcg aaccgacata aacgacagtg cgctggtgta atttttctgg aataatgtct
     1801 aaaattcttt gcgttccatt actcgcagtt ccatctgctt gttcttgtaa aaatgctaag
     1861 ggattgcatt catacatcaa tcgaagcttg ccaccaggag cgtcttttgt agctggataa
     1921 ataaaaatgc caccttttaa aagattgcga tgaaaatctg caaccatcga gccaatgtat
     1981 cgaagtgaat acgccttttt atttgtgctg tcttgacagt aatcgacaaa gctttttact
     2041 ttatcagaaa acgaattaaa attcccttga ttaatggaat atgtctttgc agttgatggt
     2101 gttttaatat tactatgcga caaacaaaat tcgccaattg taggatccaa tgtaaaacca
     2161 tttacaccat caccagttgt atatactaac attgttgaag aaccataaat aacataacct
     2221 gcagcaactt gtttgtgtcc tggctgtaaa aaatcgtcta aagtgcacaa gtcacctttt
     2281 tttgacaaac gagcaaaaac agaaaatata gtgcctacag gagcaatcac atcaatatta
     2341 ctcgagccat ctaaagggtc aaataaaacg acatattttg cattttttct attgtcaaat
     2401 gcaacaaaat cttcttcttc ttcgctggca ataccacaac attcaccact attttgcaga
     2461 cattttttca acacttcatt tgcaataata tctaattttt tcacgtcttc gccttgcaca
     2521 tttatgctat ctgcatcgcc taatatagca tcaactagtc cagctctact taaatctcta
     2581 ttcacaattt ttgctgccaa accaatatcg cgaagcaaac ccgttagctc accagtagca
     2641 tcaggcaatt cgtgttgtct tgccgctata aattcatcaa aagttatcag acgcataaat
     2701 ataaatattg caacaaagat agagagtaga actcgaaaaa tatcaaattt ttgcattcat
     2761 tttaattatt ccttaatagc cattagctta tcaacttctt tgaaataagt atcttagcag
     2821 tccaatttta aaaatggaga caaaaattct aataagaaag gcagaaccgg cagacttaac
     2881 ggcggtttat gcgcttatta aagaattagc aatatttgag aaagagccga atcagccaag
     2941 taattcatta gagaaattca ttcaagatgg cacggcaaaa cagcctcact tccatgttat
     3001 tgttgcagaa attgaagctc aaattgtagg aatggcgatg tattatttcg gctattcatc
     3061 ttggaaaggt gctatgttgt acttagatga tttaatcgtg aaagaagcat atcgcaaaca
     3121 tggtattggc aagaaattag ttgacgaatt aattaatata gccaaagaag aacgagtgag
     3181 tttgatgaaa tggcaagtgt tagattggaa tgaaaatgcc attaaatttt acgaaaaata
     3241 tccaattgta aaagatgcaa gttggattga tataaaaata gaaaaagaaa atttacagta
     3301 atgaaagtat tcaaatttgg aggtgcatca gtgaaagatg ctgcaggaat taaaaacgta
     3361 gcatcaatta ttcagaaata tggtgaaaaa aatttagtgg ttgttgtatc tgcaacagga
     3421 aagaccacaa acgcattaga agaagttata aaagcctatt ttaatgacac agatgcaaaa
     3481 gcagcatttg atgtggtgaa aaaaaatcat catcaaattt gtattgattt atttggcaat
     3541 gatacgcaca aagtatttga cagtttacaa aatatttatg ctgaagtaga ttggcaatta
     3601 gaagaaaaaa gaaaagaaag ttataattat gtgtatgacc aaatcgtatc acaaggcgag
     3661 ttaatttctt ctgttatctt aagtgcatat atgaatgaaa taggcatcaa taatacttgg
     3721 ctagatgttc gtgatgtgat gaaaactgac gacacgtaca gagaagctgt agttgattgg
     3781 aataaaacac aggaactcat gaatgcgaaa gtaaaacaaa tgatagatgg cagcatagct
     3841 gttacgcaag gttttattgg ttgcacagca gaaaactgca caacgacatt aggcagagaa
     3901 ggctctgact acacagcagc tatttttgca aacatgttag atgcagaaca tcaaactatt
     3961 tggaaagacg tgccaggcgt tatgaatggc gatccacgtg tatttgaaga tgctgtattt
     4021 atcgacgaaa tttcatttaa tgaagcagta gaaatgacat tttatggtgc taccgttatt
     4081 catccaaaaa cgataaaacc attacaaaat aaaggcattc ctttgttggt aaaatctttc
     4141 attcatcctg atggaaaagg aactgttgta ggcggaaatg ataaaaattt accgccaatc
     4201 agaattgtaa aaaacaatca ggctttaatt acattacaca caaaaaattt ctcatttgta
     4261 gaagacgaag tgattgcaga aatattttct tcctttttca aagcaaattt aaaattaaat
     4321 atgatgcaac aaggtgctat ttcctttgaa gcagtagtag ataatattcc agaaaaaata
     4381 gcaaaagtaa aagatgcgct gcaagataaa tttaacttcg ttatcgatga gaatttgacc
     4441 attcttacaa tcagacatta tactgatgct gcatacatca ataaatttaa aaacgaaaga
     4501 tcaacgatat tagaacaacg cagaccaagc acttatcaag gattgttagc ttagtttttt
     4561 tataaaaata tatagcatgc cattatttcc aataatggca tttttatttt aaaatggtga
     4621 aaaaatcaaa ttaaaacacg gcacgtaatt gtgctctgcc agtattgata agtttggcag
     4681 tgcctgtttt tctgccttca taggtaaggc ttaattctaa attctgagaa atatttcgag
     4741 tataagtact actccaaatg taatttgagc caggctgtaa tccttctaat atcgtgtatg
     4801 attttgtcgt gccattttca ccattatatt tcacttgtac aaaagaaaac ttcgcttcta
     4861 atgtgttttt aagcgcttta ttatagcgca attcgaactc taatttattt gctaatgttc
     4921 gttcaccacc aagctgtatt tcattttgtt tatgtgtaaa tccataattc acagaagtgc
     4981 gtagcattgt tttataattg aatgtaaatt ttggttcaac gtaataagca cggattaaat
     5041 aattattagg ctgaaaataa tcggaaacca aagctatttt agattgaata aatttgagat
     5101 ttagagtgaa tttttggctt aaattataat atgcttcaat aatattgtcc attcgttttc
     5161 tggtatcaaa accatttact aataatattt tatcgctcac catttgattg gtgaatgaaa
     5221 atgaataaat cggatcgcca gcattaaata taaacgagtt tcgaatattc gtattcaatg
     5281 aaataatagc agaatcagac gttttgaaca caaaaggatt aaacgctttt gcgatattac
     5341 ttttgtaggt tttacgtcga agctgcatat aagactgcaa agtaagcttt gacatgaatt
     5401 ttttaaaacc agattggtta aaccaaattg ctttaggttg tatggtaata ttttgtgtat
     5461 atgcagttga attggcatta acatattcat tcaaatcagt caatacgcgt acaaattgtg
     5521 cgctatcatt aaactgtgat ggaacaaatt cattcaattc acgaatgcca ttgtcatttt
     5581 ggtctatcca tttaaaatag ccttgtcctg aaggaacatc aacataagta aactggcgta
     5641 cgcgttctct gcctccgcct agttcataaa tgaaattcaa acgcaatacg ccttttttaa
     5701 tacgagcacc atattctgtt cttcccaaaa aagtttgttc cgcttgttgt tgtatgcttg
     5761 tatctttcac caataaattt ctataagtaa atgtccaatt aaaagtttga tttttgagtt
     5821 gattggcacc acctttaaat tctaccgtat gtgcagttgt tgagtcagaa aatgcattat
     5881 tttttacctt gctatctttc ctatatttat acgctaacat aaagttccac ttagcagaat
     5941 caggcataaa aaactgaaat gacaagttat tatttctaaa agaattattg gtcaactctt
     6001 tcgttgcaat agatttaaac acattattct cttgataaaa tcctacacca aaaccaatgc
     6061 cacgtgcttt cgcaatcgta taagatgcat tgatttgtgg tctaaaaaaa agcgacttaa
     6121 acatattatc ttgcgtgttt aataatcgag catctatgtc tattttcact tgcttataat
     6181 ccattccaaa tcgtattcta ttttcaaaac cttgatattg tttttgtcta atgaaagaag
     6241 agaaacgata agaagcggaa atattatgtt taatatcttt aagctctgtc atataattag
     6301 caatatgctc cgtataattt aatgcatttc tgatattcca atctcgacta aattcaacat
     6361 ttctatatgg ttctacttgt tgaaaattcg acgatttaaa ttcataattt aaggcatttg
     6421 taaatacaat tttatgctta aaggaatcga ttcgctgcaa ttgtacattt gctgcgtagc
     6481 ctttattttc tttttttcca attggtgaaa acaaattctt atcataattt gaaaagccca
     6541 tttctgcacg tactttcgtt ttattatcta tttgataatc ggctccaaat gtgaatagca
     6601 attgactttt tggcgttacc aatagtatca atggttcata gctaccttgt ggcacaccat
     6661 tgactggcga aatccattca taaacacgaa cgttattagc atttttagtt ggcaaataat
     6721 ttccttttcc tgtacctaaa taggaaaatg ttagcaaata ttttgcacta tcaggattgg
     6781 tttcatacac aaaaacagaa tcgtataata ttgcattgac tgttgtgtct ttcattttat
     6841 attgcacacg attagggtca aatgccacct cgcgcacacc agatacgtaa gcattgctga
     6901 tgtcattacc gattgtagat agaaataatt tattgctatc agaaatagca ttgcttatcg
     6961 gtttgctttt tgcatcttgt tctgcataaa cttgcgtaaa aatatttaat tttttccaag
     7021 aaaaccgaat tgcttcgtgc acagtcgttc taaaataatt tctatctgca tattcaaatt
     7081 caataacaat tcgagaatct ttagtaatta aaaaacgagg cataaaaatt atttcgcctg
     7141 tattataatc catgatataa tcttgcaatt caccacgttg caagcgcaca ccatctacat
     7201 acactttctc gctgccagcc aaaataatta agaaagtttc attattattt cctactaatc
     7261 tatatggtcc ttgatttcct tccgttccac taaaaacatt tcgagcaaac ttgcctctac
     7321 taacagccat agaagtggaa gtttggagat gcattccatt tttaaaaata agatgattga
     7381 cgtagctcgc tccttgcagt tgtttagcaa atttcagaaa ataactatca taatttttca
     7441 atgcataatc accagcaatt acagtatgtg gttctttctt taattgaata aacactttgt
     7501 caaaatcttg taattgttgc gtcgtaccat ctggctgcaa tggaatatta ttgtctgtca
     7561 atgatgctgt aatttcaata ccattgccaa tttttccact taatcgcaaa tctaaggttg
     7621 agtttaaagt caaatcttga ttagaaccaa attgaattcc tcgcgcaaaa tttccgctat
     7681 aatttaagcc accaaaatct aaaaaacttg ctgtctgttc tgcttttggt tgataataaa
     7741 atcgttgata aaacaaagaa tctccttttc ggattaagct tggattttta tgttgtgata
     7801 taccagccaa tgctgttgga aaagtacgat aggaaacaat aatagaatct ggtctttgct
     7861 tatttttaaa tacaattgtt gcttttgcaa aatcaaagaa aacactatcg ctcgagagct
     7921 gttcgtcatt taatagattt cgtatttgca gtgaattggg aaatatactt agtgtgtcta
     7981 gttgaatggt atctgatgta atatatacca ctttgctacg ctgattagaa tagattgaaa
     8041 aatctaatgt gcgtatttga gcatgcacgt gcatgacagt aaatacgaag caaaatatta
     8101 taattatcag tcgaaatcgc atgtagtata aatggtaaaa ataaatacat tattgtatta
     8161 agaggaattt atttactttg tagttgctta tttaattttt atgaacactt tggatttaat
     8221 aaaaaacaga agaagtatag accctgatca ttttactggt gccatggtag aacatcacat
     8281 catcgaaaat atgttagaag cagccaactg ggcgcctaca catggctata cggaaccttg
     8341 gcgattcatt gtctatgaag gcgaaaaaat cgcttcattt agtgaatttc atgcttcgct
     8401 gtatcaaaaa cacacaaatg cagaacaatt tctacaaaaa aaatatgaga aattgaaaaa
     8461 tagagctcga ttatgttctc atgtcatcat ttgcgtaaat aaacgaggag ataaaagtaa
     8521 tattcctgca ttagaagaaa tagcggcaac gagtgctgcc attcaaaata tgttgttagt
     8581 tgctaccagt aatcaggttg ctactttttg gagtactggt ggcatgtgtt atcatcagtc
     8641 gtttgccgaa gagcttggtt ttacaataga agatcaagtc ttaggaattt tatatattgg
     8701 aaaatctgaa caggtgcaat atgaaggcaa gcgaataagc gagtggcaag caatagtaac
     8761 ttggaagtaa aatacaagct acaatttacc taagatgtgg tataacatga atgtgattcc
     8821 tttgttacat ttgttattat gtcaatagaa attttccatt ttgtccgttt cttgcacgtt
     8881 gtatgtgcat ctgcttggtt tggcgaagtt gtcgtcatca attttgtatt aattccagca
     8941 ttatcgcgat atcaaggtgt ttataaaaaa gattttctaa acactatttt tccaaaaata
     9001 tttaatttgg catccgtatt agcaggcact accgcagtta ctggtggtat tttattatat
     9061 cattttattg gggttgatat tgtatcgttg acatcaaaag gcacttgggg ttggtctatt
     9121 ttaattgcag gtacgcttgg cttagtactt actatttttc atttttttat agaaaataat
     9181 ttagcaaaaa aagttggcgt tggtgaccca aacttatctc aagatagtgt agaagatgtg
     9241 cacttgaaat taaaatttat accaagggct ggcttagtgg tgattacaac aatattttta
     9301 ttaatgctca atgcaacgca tgcattgatt tcattctaat tcaaatgatt aatcgtgttt
     9361 cagcttaggc atttgcgtaa actc
//
LOCUS       DAOQQV010000102         9338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2760_length_9338_cov_7.199935, whole genome shotgun sequence.
ACCESSION   DAOQQV010000102 DAOQQV010000000
VERSION     DAOQQV010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2760_length_9338_cov_7.199935"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..609
                     /locus_tag="PLL59_09835"
     CDS             190..609
                     /locus_tag="PLL59_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24149.1"
                     /translation="MKTTTKIFMSAIFSAALLFASCKKEDDKTIKPGTPSIIGTWNFD
                     GGTFHGSILSKNSPVNTFNFRSDNKLERKLDLNYSTGCPDRSCTGFILPYKYNDTTSY
                     VMSASDVTFDGGTWQSTITNSTLILKKGGIDSLHFSR"
     gene            complement(682..2322)
                     /locus_tag="PLL59_09840"
     CDS             complement(682..2322)
                     /locus_tag="PLL59_09840"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMY24150.1"
                     /translation="MELIEQLKLKLNEESLTLEEKLDTLITLTANLMQYDVEMALQYN
                     KQAEELSSHFPPDNIQRGLILNNYGVYYYMVNLNELALEKFIAADKILSKSNNVEHHI
                     LSKADMALIYTRTEQYQEALDQYLKIEKEIATLPLSIRHAQIYVNIDAAYVYLKDYKN
                     ALKYSLKGLEIVENENHIFERAISYVNTGGNYLQLNEIEIAKTYIDKSNALIEEHAIE
                     SLASSINLKYAEYHAKINDFQQAVSYGNKALKAAERKKSEEQMMFICGRLIDFNEQIA
                     DYKQALAMSKKYNEMKHAMLTRDKMNILNALQIQYNIEKKELELSALKIKQQELELEK
                     RDSELAALKSQMNPHFIFNALNSIQELYTIGDKKIANEQMGNFSSLTRKILDVSGKQK
                     ITLAEEIEILTKYLELESMRFEMDFAYTVSFDEKIDEDYLQLPPMLIQPFVENSIKHG
                     LLHKKGMKKLSISFEVNDAEDILLCTIIDNGIGRDASAQINKNRVASHVSFASAATQK
                     RLELLNKDKEKRVAVVFEDVIDEQQQIAGTKVILQIPL"
     gene            2369..2881
                     /locus_tag="PLL59_09845"
     CDS             2369..2881
                     /locus_tag="PLL59_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24151.1"
                     /translation="MINKLKHIIFYFYLRFKNKKLAERQPISLKNAQHIGIIFQAENI
                     QQNDSILAFSKKLKELHKDVQLLGYMPKREFGFQYPFPFISNKETSWYGKPGGGTSGF
                     FTRSPFDLMINFCSEDCLPLEYISAVSDAKFRVGFNKDINNANYDLILISNEKQNISN
                     LIQNLEKYLS"
     gene            2939..3820
                     /gene="dapA"
                     /locus_tag="PLL59_09850"
     CDS             2939..3820
                     /gene="dapA"
                     /locus_tag="PLL59_09850"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HMY24152.1"
                     /translation="MLNLKGVGVALVTPFNEQKEINFAEFEKVVNYVIEGGVDYVVVL
                     GTTGESVTLNKKEKQDLINACVSATNKRVPVIAGFGGNDTRSTIADINGFELNGVDGI
                     LSVSPYYNKPTQEGIYEHYKAIATNSNLPIVLYNVPGRTASNIRATTTLTLAHEFKNI
                     IAIKEASNDWQQIFWLAREKPADFLLISGNDDLIVPQISIGYDGVISVIANALPKQFS
                     GMVHQALNGDFTAARKTVYELDELITYLFEQGNPAGVKAAMQHLTVCNDHVRLPLMPI
                     NTDLRQKIGKKLESLLK"
     gene            complement(3836..4075)
                     /locus_tag="PLL59_09855"
     CDS             complement(3836..4075)
                     /locus_tag="PLL59_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24153.1"
                     /translation="MKKYNLIFLSNAKLQLSKRFFNSWQEIQSFYEDYMTSIEFNSLE
                     EVNDYLCIEYNLTIEQASILTAKINAAENDIELMF"
     gene            complement(4084..5268)
                     /locus_tag="PLL59_09860"
     CDS             complement(4084..5268)
                     /locus_tag="PLL59_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HMY24154.1"
                     /translation="MSKLDFNNTQQAFAYKSNSELIRDYQIFRLISLSWLVKFGTKAA
                     ASLIHAGVKTPVAMGMKPTVYATFCGGNTLTEASKKINNLHKYHVQCVLDYGVEGKDS
                     EADFIRTENAIKEAILFATHNPAVSIVCSKFTGLIPFSILEKLHAQTALSDDEHAVFQ
                     KSKARIYSIAQLAADNNISLFVDAEESWIQKPLDDLTYELMAKFNKEKPIIYNTIQFY
                     RKDRLAFLKQEVARAKVDGFIYAVKLVRGAYMEKEATRAAQLNYENPIQNSKEHTDAD
                     YNDGLRYAIENIEHTAICVATHNELSTQLAIDLMQQKSLANNHTHIHFSQLYGMSDNI
                     TFNVAAAGYNTHKYLPYGPVKDVIPYLIRRAEENTSVAGQMGRELKLLKTEVKRRKLF
                     VL"
     gene            complement(5349..6089)
                     /locus_tag="PLL59_09865"
     CDS             complement(5349..6089)
                     /locus_tag="PLL59_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HMY24155.1"
                     /translation="MKQFGLIGFPLSHSFSKGYFTDKFINEQIHDCAYDVFPLAKIED
                     FVSLCEERNNLQGLNVTIPYKQAVIPFLDELDSEAAAIGAVNTIKFSQGKKIGYNSDC
                     YGFEMSLQPLLQAHHTAALIMGTGGASKAVEYVLQKLGIAFQYVSRTKQENVITYTEI
                     DKSVMQHYTLIVNTSPLGMYPNVDAAPEIPYEYIDKQHLLYDLVYNPVETRFLYEGKI
                     RGAQIKNGLEMLHLQAERSWQIWNDIAQ"
     gene            complement(6153..7568)
                     /locus_tag="PLL59_09870"
     CDS             complement(6153..7568)
                     /locus_tag="PLL59_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY24156.1"
                     /translation="MSNFDFENENDNQLNITELIERYERSEKVGQTPFFDQDDFEAII
                     EYYQSKGLFEQALVVVEKSMLQFPYSSILLIKKAQIYFELKQLNIALEILDKAVIYDS
                     SEIGIYLLRAEIYTFQSRYKEATEILYDLLSTAEKDDLADIYLQLCDVYEDWEKYFNV
                     YDCLIECLKVDPNNEEALNRFNYCIEIVDKFEESIPFHNYLIDINPYNEFAWYNLACA
                     YRGINEYEKAIDAFEYVLAINEDADFVYQDIAELHFKNGMYKNALEVIRDMCETFEAD
                     DEIYFLQGKCHEALGNNKMARYCYRKAVHDNPSLSEAYFRIGETYKNEGMWEQAYKSF
                     QKANELEKEQYDFCLAMAEAAMEIGETEVAIDACETAIDIFVKRHEAYFILGKIMAQY
                     GDIEIARDILMKGAEVCKSTIELNYAICATAFMENKLKEAEVMLRLLLAEDYSKYDVL
                     FDFCEDLEENQAIQNILANFN"
     gene            7675..9033
                     /locus_tag="PLL59_09875"
     CDS             7675..9033
                     /locus_tag="PLL59_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24157.1"
                     /translation="MKYSLIVINILVVFTYNCVYGQDTSAVPIDSSAISASEENVYVK
                     KQKKERHYPKLQAAQEKYLHWIDKRHREFPFGVPAIAYNKYDGVQVGAALINLKQPVK
                     NVDFTASLLYGIKSKKVNGTVNIDYYIKPKKSVVSEIKPSLRFQSFSYYNYVKPLKYY
                     AIHPEISIAFNHRTEKLQQLEHRLKFTSHVIMHKEIAYHAIDSVTVELKDTLTHYYVN
                     VLNYTFKRNDKNFPFEASMTFEQSKNFVKTYFEANSFIRYQLKDYNTGVHLRFFIGGF
                     LWRNNNYRFRLYPDVGMSLNGKRGEQDYLYNDTYFGRNEQANFAGRQITKTDGFFKVS
                     TPLNAIEIGQTADWLMAFNFKIDFPIKYVPIKLFFDLGYSFDNHLNPINLLPMKGFQY
                     DGGVMFSFFDEGFEIYLPLFYSKEYKTYYKANAPKFGQRISFLFDLHKIELHKKIRGM
                     RF"
BASE COUNT         3001 a         1611 c         1438 g         3288 t
ORIGIN      
        1 aaaatatatt caaaaaacac tttaacaatg ctataaaaca tgaaaagaaa ttgacacaca
       61 cttttaataa aacaaagttg ctgtttataa agtcaaaact accatccgtg atatggaata
      121 aaatagagca tcagattcga tgcatatttg caacatcaaa ataattaatc acaaatcaaa
      181 aaaatgaaaa tgaaaacaac aacaaaaata tttatgtcag caatattttc tgctgcatta
      241 ttatttgcat cttgcaaaaa agaagacgat aaaacaataa aacctggaac acctagtatt
      301 attggtactt ggaattttga cggtggtaca ttccacggtt caattctatc aaaaaattca
      361 cctgtaaaca cttttaattt cagaagtgac aacaagctgg aaagaaaact agatctaaat
      421 tatagcactg gttgtccaga tcgttcatgt actggtttta ttttaccgta taaatacaat
      481 gacacgacat cttatgtcat gtctgcatca gatgtaacat ttgatggtgg cacatggcaa
      541 tcaacaatca caaattctac attaatatta aaaaaaggag gcattgacag cttgcatttt
      601 tcaagataaa aagacacgaa aaaaaatcat tatgttttat gaatgttttt aagtggcgat
      661 gtcttttgca tcgccatttt tttacaatgg aatctgaaga atcactttag ttcctgcaat
      721 ttgttgctgt tcatctatca catcttcaaa aactacagca actctttttt ctttatcttt
      781 gtttaataat tctaaccttt tttgcgttgc tgcgcttgca aatgaaacat gtgatgcaac
      841 acgattttta ttaatttgtg cggatgcatc tctgccaatt ccattatcta taatcgtaca
      901 caataagata tcttccgcat cgtttacctc aaacgaaatg gataattttt tcatgccttt
      961 tttgtgcaaa aggccatgtt ttattgaatt ttcaacgaat ggttgaatta acattggagg
     1021 caattgtaaa tagtcttcat caattttctc atcaaaagaa actgtgtagg caaagtccat
     1081 ttcaaatcgc atgctttcta attctagata ctttgtcaat atttcaattt cttctgctaa
     1141 tgtaattttt tgtttgcctg aaacatctaa tattttacgc gtcaatgaag agaaatttcc
     1201 catttgttcg ttggctattt ttttatcacc aatggtatat aattcttgaa ttgaatttaa
     1261 agcattaaaa ataaaatgcg gattcatttg tgattttaat gctgccaact cactatctct
     1321 tttctctaat tctaattctt gttgttttat ttttagtgca cttaattcca attctttttt
     1381 ctctatatta tattgaattt gcaaggcgtt cagaatattc attttatcac gtgtcaacat
     1441 ggcatgcttc atttcattgt atttttttga cattgccaat gcttgtttat aatctgcgat
     1501 ttgctcatta aaatcaatta atcttccaca aataaacatc atttgctcct cagatttttt
     1561 acgctcggca gccttcaatg ctttgtttcc atagctgacc gcttgttgga aatcattaat
     1621 tttagcatga tattcagcat atttcaagtt aatactactc gccaaacttt cgattgcatg
     1681 ttcttcaatt aatgcattcg atttgtcaat gtaagttttg gcaatttcaa tttcattcaa
     1741 ttgcaagtaa ttgccacctg tatttacata agaaattgct cgttcaaaaa tgtgattttc
     1801 attttctaca atttccaatc cttttaaaga atatttcaat gcatttttgt aatcttttaa
     1861 atacacatac gcagcatcaa tatttacata aatttgtgcg tgccgaatac tcaaaggcaa
     1921 tgtggcaatt tctttttcta tttttaaata ttgatctaaa gcttcttgat attgttctgt
     1981 tctggtatag attaatgcca tatctgcttt agacaaaata tgatgctcaa cgttattact
     2041 ctttgacaat attttatcgg cagcaataaa tttttcgagc gcgagctcgt tcaaattcac
     2101 catgtaataa tacacaccat aattattcaa aatcaatcca cgctgtatgt tgtctggcgg
     2161 aaaatgtgac gataactctt cggcttgttt gttatattgc aatgccattt ccacatcata
     2221 ttgcatcaaa ttggctgtta atgttattaa agtatccaac ttttcttcaa gcgttagcga
     2281 ctcctcattg agcttcaatt ttaattgttc aatcaattcc aaaatccgat atttttattt
     2341 atacaatttc tttgtgtaac tttagcacgt gataaacaag ctcaaacata tcatttttta
     2401 tttctatttg cgctttaaaa ataaaaaatt ggcagaacga cagccaattt ctttaaaaaa
     2461 tgcccagcat attggcatca tatttcaagc tgaaaatata cagcaaaacg acagtattct
     2521 tgcattttca aaaaaattaa aagaattaca taaagacgta caattattag gctatatgcc
     2581 aaaacgtgaa tttggatttc agtatccatt tccttttatc agcaacaaag aaactagttg
     2641 gtatggcaaa cctggtggtg gcacttcggg atttttcaca cgttcaccat tcgatttgat
     2701 gattaatttt tgtagcgaag attgcttgcc attagaatat atatctgcgg tttctgacgc
     2761 aaaattcagg gttggtttta ataaggatat caataatgct aactatgatt taatattgat
     2821 ttcaaacgaa aaacaaaata tttctaattt aattcagaac ttagagaaat atttgagctg
     2881 agaagtggat agtgaggtgt gtaattatga ataattataa ataattataa aaatatttat
     2941 gttgaatttg aaaggcgtag gcgtagcatt ggttacacca tttaatgagc aaaaagaaat
     3001 taacttcgct gaatttgaaa aagtagtcaa ttatgtaatt gaaggtggcg ttgattatgt
     3061 tgtcgtatta ggtacaactg gcgaatctgt cacattaaat aagaaagaaa aacaagattt
     3121 aatcaatgct tgtgtaagcg caacaaataa gcgcgtgcct gtaattgctg gttttggtgg
     3181 caatgacacg cgtagcacta ttgctgatat taatggattt gaattaaatg gcgttgatgg
     3241 tattttgtct gtgtcgccat attacaacaa acctacgcaa gaaggtatct acgagcacta
     3301 taaagcgatt gcaacaaaca gcaatttacc aatagtatta tataatgtac ctggcagaac
     3361 ggcatcaaat atacgtgcga caacaacatt gacgcttgct catgaattca aaaatattat
     3421 tgctataaaa gaagctagca atgattggca acaaattttc tggttagcca gagaaaaacc
     3481 agcagacttc ttattaatca gcggtaatga tgatttaatt gtaccacaaa tttctattgg
     3541 ttatgatggt gttatttctg ttatagcaaa tgcacttcca aaacaatttt caggcatggt
     3601 gcatcaagca ttgaatggtg atttcactgc tgcacgcaag actgtttatg aactggacga
     3661 gctcatcacc tatttgtttg agcaaggaaa tcctgctggt gtgaaagctg ctatgcagca
     3721 tcttactgtt tgcaacgacc atgtgcgatt gcctttgatg cctataaaca ctgacttacg
     3781 ccaaaaaatt ggtaaaaaat tggaaagctt acttaagtaa gttgatacaa aaaatctaaa
     3841 acattaattc aatatcgttt tcagcagcgt ttatttttgc tgttaatata cttgcttgtt
     3901 ctattgtaag attgtattca atacataagt aatcattcac ttcttctaaa gagttaaact
     3961 caatagatgt catataatct tcatagaaag attgtatttc ttgccaagaa ttgaagaatc
     4021 tttttgacag ctgcaactta gcattactta aaaatatgag attatatttt ttcatgaata
     4081 aaattaaagc acaaacagct ttcgtctctt cacttctgtt ttaagtagtt ttaattcgcg
     4141 acccatttgt ccggcaacgc ttgtgttttc ctctgctcta cgaattaaat aaggaatcac
     4201 atcttttact ggaccataag gcaaatattt gtgtgtatta tagcctgctg ccgccacatt
     4261 aaaagtaata ttatcactca tgccatacaa ctgtgagaaa tgtatatgtg tatgattatt
     4321 tgcgagtgat ttttgttgca ttaaatcaat tgccaactgt gtgcttaatt cattatgtgt
     4381 ggcaacgcaa attgcggtat gttctatatt ttcaatagca tatctaaggc catcattata
     4441 atcagcatct gtatgctctt tggaattttg aataggattt tcgtagttta attgtgctgc
     4501 acgcgtcgct tctttttcca tgtaagcacc acgaactaat tttactgcat atataaaacc
     4561 atcaactttg gcacgtgcaa cttcctgttt taaaaatgcc aatctatctt ttcgataaaa
     4621 ttgaattgtg ttataaataa taggtttttc tttgttgaat tttgccatca attcataagt
     4681 caaatcatct aatggctttt gaatccaact ttcctccgca tctacaaaca agcttatatt
     4741 attatcggct gctaattgcg caatgctata aatacgcgct tttgattttt gaaaaacagc
     4801 atgctcatca tcgcttaatg cagtttgtgc atgtaatttt tctaagattg aaaatggaat
     4861 tagacctgta aatttagaac aaacaataga cactgctgga ttatgagtgg caaataaaat
     4921 agcttctttt atcgcatttt ctgtgcgtat aaaatcagcc tcactgtctt tgccttctac
     4981 gccataatct aaaacacatt gcacatgata cttatgtaaa ttattgattt tcttgctggc
     5041 ttctgtcaat gtatttccgc cacaaaatgt agcatatacg gttggcttca tgcccatggc
     5101 aactggtgtt ttcacgcctg catgtattaa gctggctgcc gctttggtac caaattttac
     5161 caaccaactt aaactaatta aacgaaatat ttgatagtct cttatcaatt cgctgttgga
     5221 tttgtaggca aacgcctgct gtgtgttatt gaaatctaat ttagacatat tggtatgaca
     5281 ttagatagga aataagtatg gcaaaattac tattattttc aataacagta tcattacttt
     5341 atgctaattt attgtgcaat atcattccag atttgccaac ttctctctgc ttgcagatgc
     5401 aacatctcca atccattttt tatttgtgct cctcttattt tcccttcgta tagaaatctt
     5461 gtttctactg gattgtaaac caaatcgtat aacaaatgtt gtttatcgat atattcgtaa
     5521 ggaatttcag gtgctgcatc tacattcgga tacatgccta atggtgaagt attaacaatc
     5581 aatgtataat gctgcataac tgatttatca atttctgtat aagtaattac attttcttgt
     5641 ttagttctag aaacatactg aaatgcaatg ccaagctttt gcagcacata ttcgacagct
     5701 tttgatgcac caccagtgcc catgattaat gcagctgtat ggtgtgcttg tagcaatggt
     5761 tgcaagctca tttcaaatcc atagcaatct gaattataac ctattttttt tccttgcgaa
     5821 aatttgattg tatttacagc gccaatggct gccgcttcgc tatctaattc atctaaaaat
     5881 ggtattactg cttgtttgta tggaatggtt acgtttaagc cttgtagatt atttctttcc
     5941 tcacacaaac taacgaagtc ttctattttt gctaatggaa atacatcata cgcacaatca
     6001 tgtatttgtt cattaataaa tttgtctgta aaatatcctt ttgaaaatga gtgtgaaagc
     6061 ggaaaaccga ttaatccaaa ttgtttcatg ttcgtctctc gagtataata atttctcgca
     6121 agcgagaatt tatactattt tttttgctgt tattaattaa agtttgccaa aatattttgg
     6181 atggcttggt tttcttctaa gtcttcgcag aaatcgaata atacatcgta tttactataa
     6241 tcttctgcca gtaatagccg aagcatgact tccgcctctt tgagtttatt ttccataaat
     6301 gctgtagcgc aaattgcata attcaattct atcgtgctct tgcacacttc tgcacctttc
     6361 attagaatat cacgtgctat ttcgatatca ccatattgcg ccattatttt acctaatata
     6421 aaataggctt catgacgttt tacaaaaata tcgattgctg tctcgcaagc gtcgatggca
     6481 acttctgttt cgcctatttc cattgctgct tccgccatgg ctaagcaaaa atcgtattgc
     6541 tctttttcga gttcattcgc tttttggaaa gacttataag cttgttccca catgccttcg
     6601 tttttatagg tctctccgat tctaaaataa gcttcagaca gcgatggatt atcatgcact
     6661 gccttgcgat agcaatatcg cgccattttg ttattgccta aagcttcgtg acattttcct
     6721 tgtaagaaat aaatttcatc gtctgcttca aaagtttcgc acatatctct gatgacttct
     6781 aaagcatttt tatacatgcc atttttaaaa tgcaattctg caatatcttg atatacaaaa
     6841 tctgcatctt cattaatggc aagcacatat tcgaaagcat ctattgcttt ttcatattca
     6901 tttattcctc gataagcgca agctaaatta taccatgcaa attcattgta tggattaatg
     6961 tcaatcaaat aattgtgaaa tggaatgctc tcttcaaact tatctacaat ttcgatacaa
     7021 tagttaaatc tattcaatgc ttcttcatta ttaggatcta cttttaagca ttctatcaaa
     7081 caatcataga cattaaagta cttttcccaa tcttcataca catcgcataa ttgcagatag
     7141 atgtctgcga ggtcatcttt ttcggccgta ctcaacaaat cgtataatat ctctgtagct
     7201 tctttgtagc gcgattgaaa tgtatatatt tcagcacgta acaagtaaat accaatttcc
     7261 gaactgtcgt aaatgactgc tttatctaat atttcgagcg caatatttaa ctgttttaat
     7321 tcaaaatata tttgcgcttt tttgataagc aaaatagaag aatacggaaa ttgtagcatt
     7381 gatttttcta caaccactaa agcctgttca aacaatcctt tagactgata atattctata
     7441 atggcctcaa aatcatcttg gtcaaaaaat ggtgtttgac ccactttttc tgagcgttcg
     7501 taacgctcta tcaactcagt tatatttaac tggttgtcat tttcattttc aaaatcaaag
     7561 ttactcatag gcactatctg tttttatgac aactcgtaac aacaaataca aggcaatttc
     7621 aaagttaata cttaatattg tgttacattc tatttcaaaa gttaattgtt aaaaatgaaa
     7681 tatagcttaa tagttattaa cattttggta gtattcacat ataattgtgt ttacggacaa
     7741 gatacaagcg ctgtaccaat cgacagttct gctatttctg catctgaaga aaacgtttat
     7801 gttaaaaaac aaaaaaaaga aaggcattat ccaaaattgc aggctgcaca agagaagtat
     7861 ttgcattgga tagacaaacg ccatcgagaa tttccgtttg gtgtgccggc aattgcttat
     7921 aataaatatg atggcgtaca agtaggtgca gctttaatca acttgaagca gccagttaaa
     7981 aatgtagatt ttacggcatc gcttttatat ggtattaaat caaagaaagt gaatggcact
     8041 gtaaatatcg actattacat aaagccaaaa aagagtgttg tctctgagat aaagccgagc
     8101 ttgcgttttc aatcttttag ttattataac tacgtaaagc cattgaaata ttatgcaatt
     8161 catccagaaa tttccattgc atttaatcac cgaactgaaa agttacaaca attagaacat
     8221 cgtttaaaat tcacatctca tgtgattatg cataaagaaa ttgcctacca tgcaattgac
     8281 agtgttactg ttgaattgaa agatacgcta acgcattact acgtcaatgt tttgaattat
     8341 acatttaagc gaaatgataa aaattttccg tttgaggctt ccatgacatt tgagcaatca
     8401 aaaaattttg tcaaaaccta ctttgaagca aattcattta tacgctatca attaaaagat
     8461 tacaatacag gtgtgcattt gcgatttttt attggcggat ttttatggcg taataacaat
     8521 tatcgattcc gcttgtatcc tgatgttgga atgtcattaa acggaaagcg aggcgaacaa
     8581 gattatctat ataatgacac ctattttggc agaaatgagc aggctaattt cgcaggccga
     8641 caaatcacaa aaactgacgg atttttcaag gtgagtacgc cattaaatgc catagaaatt
     8701 ggacaaactg ccgattggtt gatggctttt aattttaaaa ttgatttccc aataaaatat
     8761 gtgcctataa aattattttt tgatcttggt tattctttcg ataatcattt aaatccaatt
     8821 aatttattac cgatgaaagg ctttcagtat gatggtggtg taatgtttag tttcttcgac
     8881 gaaggttttg aaatttattt gccattattt tacagcaaag aatataaaac gtattacaaa
     8941 gccaatgcgc ctaaatttgg gcaacgcata tccttccttt ttgatttaca taaaatcgaa
     9001 ctacataaaa aaattagagg aatgagattc taatattttg aagctacttc aaatgaatac
     9061 aacaaaaaag tcctgaaaaa tcaggacttc attggtattt ttatgtttct acgactttat
     9121 ttctatacca aaattaagac gattattttt aactagtctt gatatgtatc aagattggaa
     9181 aaattacaag caaaaatgta ctttatttga catacatcat aattattgca actttatagc
     9241 aagaagaatg ttaaaatata cacttttttg tgtacctatt ttctagaaat tggaaaagtc
     9301 atacaatgtg gacctccgcg tgcacgcgac aattcggc
//
LOCUS       DAOQQV010000103         9337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2761_length_9337_cov_8.237018, whole genome shotgun sequence.
ACCESSION   DAOQQV010000103 DAOQQV010000000
VERSION     DAOQQV010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2761_length_9337_cov_8.237018"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLL59_09880"
     CDS             complement(<1..1157)
                     /locus_tag="PLL59_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HMY24158.1"
                     /translation="MQINSEIQTLHKVIVHEPDLGIEHISPEIAEQLLYDDIVFLPRM
                     IDEHFQFTQTLKQLIGNENVYEFTDLLSDVLQKDAVREELIEYLFEKEDFSLGRYETL
                     KQLASHDLASTLISGIHPETKASILAPLPNLVFTRDIGCVINDHVLICKANKKARLRE
                     NFLTKFIIHHHTLFSDFKNKIIDFVTDENIAEDSGISIEGGDVMLVSPRHILIGESER
                     TTLDAIFELKALLFEKNIVDYVTVVEIPNERYCMHLDTIFTLVSEDTCVGFSPLLFEK
                     NDKVDVITYSKDNARAVLHPTLKDLIKEMYPNMNFIACGGGISPFAQREQWTDGCNFV
                     TAKANVAYSYERNIKTLQSFRDANFTTISAKRLLSEELSLSQLDRTIITLSS"
     gene            complement(1236..1961)
                     /locus_tag="PLL59_09885"
     CDS             complement(1236..1961)
                     /locus_tag="PLL59_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HMY24159.1"
                     /translation="MFRHHGKTRTYSHNLKIASVLSIVAGIVNVTGFFAVHELTTNVT
                     GHFAYFMDNIVKYDISNGIVYLLYVFFFFSGSFMSSFLIEIISRKGERFVYIIPATIE
                     IIILLLLGVFGHYLILHYPSSIAFGLLFSMGLQNSLVTTISNAVVRTTHLTGLFTDLG
                     IELSQLFFYKKIEQKQKLMASIRLRITIILSFFVGGVLGGLLYMQIHMHTLFIAAAIL
                     LAGLIYDNIKIRLLLLRRKLRNY"
     gene            complement(1977..3044)
                     /locus_tag="PLL59_09890"
     CDS             complement(1977..3044)
                     /locus_tag="PLL59_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24160.1"
                     /translation="MKSRMVLLLKLIIGLGILFGVYVIVAILYAQFTDYKPKAEEQAE
                     VLQAKEPLPLIDKDTLVFYNWNIGYAGLGKESDFFYDGGNMVRSTKALTDKNFDGILS
                     TINSWKHDADFIEIQEIDRDSRRSYHRDQLKEVFDLLGNYSAAFGKNYDVKYVPIPFT
                     NPMGKVLGGIATLYKYKAAATPIKYAFPSNFSWPKGLFFLDRCFVKHHFNLKNGKQLI
                     VINTHNSAYDGGQLKKQEMKYFKKYIVEEYAKGNYVIVGGDWNQIPPGYTPLEANSDY
                     EEMPVPADFADKDWTFAFDKNTPTNRKVDKPYEAGKTYTTVIDFFFLSPNVELVKVEG
                     MPNGFEYSDHQPVKMICRLKD"
     gene            3166..4002
                     /locus_tag="PLL59_09895"
     CDS             3166..4002
                     /locus_tag="PLL59_09895"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HMY24161.1"
                     /translation="MKNTVLQWQSNDGVKIFGQQWENDTQPAKAVICLVHGFGEHSSR
                     YEHVAQFFTDNQYAVITYDARGHGKSEGKRGHFASYDEFLNDVNNLLKQADIHFPNLP
                     IIIYGHSMGGQVVANFAIKHQPKVAGIILSSPFFQPGFAPPALKIAAGKIMRNLIPSF
                     SLPSGLDVNAISRDKEVVKKYSNDPLVFDSISSKMGIELIEFGAEAVQNAAKLSLPTL
                     LFHGTADQLTSYNDSKKFAANAGKNVKFITYDGFYHECHNEPEKQVVLQNILDWCNKL
                     IG"
     gene            4140..4526
                     /locus_tag="PLL59_09900"
     CDS             4140..4526
                     /locus_tag="PLL59_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMY24162.1"
                     /translation="MNIFVANIERKVTDEQLQDLFSQHGEIASLKLIRDRDTGVSKGY
                     AFVEMPNDDEAQKAIDALNGYEIDGRALAVNEARPKTEYKKSGGGGGGYKGGGGGGYN
                     KGGYGGGGGYNKGGYGGGDGGYKKRY"
     gene            4657..8229
                     /locus_tag="PLL59_09905"
     CDS             4657..8229
                     /locus_tag="PLL59_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HMY24163.1"
                     /translation="MTAHSFLDKLLTRLKSGDARSIHLNALPGNFARLDIYDLINIES
                     SLHLKFLSTLLSKRDFKFSITIDATSEKNKTAEEKKIVQKIIKRLNHLDYQEKEEFAE
                     HGFLSFGFGYPLLIKRDPNNEDRIIKAPILIWYLSIEKDTRRNNTWTISRNEEHPLLF
                     NELLQAHFENNEKIKTDELEELIEDDFIDETQLLQFCKSLLDKLNIPFDTVETIATLV
                     PASNKETIDNNTKAAPWIRWSGILGLFKMQKQSIIKDVELLMQYENIIDSENNNYFDG
                     EILSPVTLDPSQENVLQQIKQHDNIIIQGPPGTGKSQSLTAIITQALLNQQKVLVVCE
                     KRTAMEVLYNNLKKENLEHLCVLIEDVYSDRKTIVERVRLHIENAEHDDLRFRSNEYE
                     LARSKFLTLQEEINHRINFSNNVIFGDDNWLELLQKGKELNQNKSHSEKANALIKLIR
                     NSDYVFTYNEFIELSKILKNAAELYQLLSNEAFIFDAIQDDNFEKIDNSENLKTRILQ
                     LQQDINSLSTSITNNIQLYNNDYDVQTGFTAFKVKLLSYFSNKYKNIIQQKENTKASL
                     YQLIQQFNQYDFIPIDLSIDVANEKISNVIPAIQNIQITCDDYVQQIQHLPAYFAFRK
                     FKLSQKTATQNLIQALIQIKCDEWCAVFETYYINQAILKIAMENGIKDDSQKLYEELV
                     LADIHCKQKLAEKINHTWKIMQQESIKTKDLTAIKYLYNQRKNKQFSSKNSLRKIVHQ
                     DFDFFTTLFPVVMVNPSVCTSILPLQKNLFDFIVLDEASQLRVEDTYSSLLRGKTKII
                     SGDKHQMPPSNFFGSETIFWNKEEEDDADSYLAESKSLLEYAEDANFKSTYLDYHYRS
                     LHPSLIQFSNHAFYQSRLIPLPEKENYQAIHFTSVDGIYKDGTNIAEANAIVDLVFSL
                     ESWKDNFPSVGIATFNIFQRDLIYDLLFEQAYKDNAKNEKLQALLNQGLFVKNLENIQ
                     GDERDVMILSSTFGKDEQGKFRQFFGPLTQEKGYQLLNVIITRAKHSLHVYTSVPETT
                     FSLFEEELAIKGNKGKSIFYAYLSYVKACVDKNQTQIDYIKSCLQKNNPPMLVHKKQR
                     TSILKESVFNALQNEFGENVQLNFALGGFVLDIVLLKDNKPYLHIDFENTNLYHHEVT
                     YRIKLHQQKIVEQYHIKTYHLWSYNWWNAAENEIKNISDCYLSN"
     gene            8329..>9337
                     /locus_tag="PLL59_09910"
     CDS             8329..>9337
                     /locus_tag="PLL59_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HMY24164.1"
                     /translation="MKKHLTRRKFLKLTSVAGVAASLPLSGCKKEPIKVDDVDIEAIV
                     VGSGFGAAVSALRLTQAGVKTLMLEMGKQYDVDATPNPFTKTLSPDGRSTWLKRRTIL
                     PVTSIQFDIPKYVGVLDRVEYNTSYMRIFRGTCLGGGSVVYGAMLPYAQPSMWNEYFP
                     YVSYDDMLNKWYPKVKNILDFSVIPDDILNSKYYKYARVGLEHCENAGMEKVMLNAGV
                     NFNTIRSEIDGTTQKCSIDNQLLYGANNGFKNSLDRNYIPQANATGNLTIETLHKVDS
                     VRQLSNKNYELTVHKINEQGETVAVKIYTCKYLFINAGVVGTMNILLKSKHLGGLANL
                     SD"
BASE COUNT         3354 a         1546 c         1539 g         2898 t
ORIGIN      
        1 gatgacagag tgataatggt tctatcgagc tggcttagtg aaagttcttc gcttaataat
       61 cgcttggcac taatcgtcgt aaaattggca tctcgaaagg attgaagtgt tttgatattg
      121 cgctcatacg aataagcaac atttgctttt gccgtcacaa aattgcagcc atctgtccat
      181 tgttcgcgct gagcaaatgg agaaatgcca ccgccacaag caataaaatt catgttagga
      241 tacatttctt ttatcaaatc tttcaacgtt ggatgcaaga cggcacgtgc attatctttt
      301 gaataggtaa taacatcaac tttatcattt ttttcgaaaa gcaatggtga aaaaccaaca
      361 catgtatctt cgctaactaa tgtaaaaata gtatcgagat gcatgcaata tctttcattt
      421 ggaatttcta caacggtaac ataatccacg atattttttt caaaaagcag tgcttttaat
      481 tcaaaaattg catctagcgt agtgcgctca ctttcgccaa ttaatatatg tcgcggactc
      541 accagcatca catcgccacc ttcgatagaa atacctgagt cttctgcaat attttcgtct
      601 gttacaaagt ctattatttt atttttaaaa tctgaaaata atgtgtgatg atgaataatg
      661 aattttgtaa gaaaattttc acgcaaacgt gcttttttat tggctttaca aatcaaaaca
      721 tggtcattga taacgcaacc tatatcacgc gtgaaaacaa gatttggtaa tggtgctaaa
      781 atagaagctt tcgtttcagg atgtatacca gagattaatg ttgatgccaa gtcatggctg
      841 gctaattgtt ttaatgtctc atatcttcct aaactaaaat cttctttttc gaataggtat
      901 tctattaatt cttcgcgtac agcatctttt tgtaaaacat cactcaatag atctgtaaat
      961 tcatatacat tttcatttcc gattaattgt tttaatgtct gtgtaaattg aaaatgttca
     1021 tcaatcatac gaggcagaaa aacaatatca tcatacaata attgctctgc tatttctggt
     1081 gaaatatgtt caatacctaa atcaggttca tgtactataa ctttatgtag tgtttgtatt
     1141 tcagaattta tttgcataaa aaatattaaa ccatacaaca atctgtatta agtaaagata
     1201 caaagaaatc atgctaaaga aaaaagaaag attgctcaat aatttctgag tttgcgacgc
     1261 aataatagta atctaatttt tatgttatca taaatcaatc ctgcaagtag aatagcagcg
     1321 gcaataaata aggtgtgcat atgtatttgc atatacaaca agccacccaa aacaccgcca
     1381 acaaagaagg aaagtataat ggtaattcgc aatcgaatgg aagccattag tttttgtttt
     1441 tgctctattt ttttatagaa aaacaattga gataattcga tgcctaaatc tgtaaaaagt
     1501 ccagtcaaat gagtagtgcg caccactgca ttagaaatag tagtcactaa tgaattttgc
     1561 aaacccatgg aaaaaagcaa gccaaatgct atgctacttg gataatgtaa gataagataa
     1621 tgtccaaaaa cgcctaacaa tagtaggata attatctcga ttgttgctgg aataatatat
     1681 acaaatcgct ctccttttct ggaaataatt tctattaaaa agctggacat aaaagagcca
     1741 gaaaagaaga agaaaacata caataaatag actataccat tgctaatatc atacttaaca
     1801 atattatcca taaagtatgc aaaatgacct gtaacattag tggttaattc gtgaacagca
     1861 aaaaaaccag ttacattgac aatacctgca acaatagata atacagaggc aatttttaaa
     1921 ttatggctat acgttcgtgt ttttccgtga tgcctaaaca tggtatttca attttcttaa
     1981 tcttttaatc tgcaaatcat ttttacaggc tgatggtctg aatattcaaa accatttggc
     2041 attccttcga ctttaactaa ttctacatta ggcgacaaga aaaagaaatc gataacagtt
     2101 gtgtaggttt ttccagcctc gtatggctta tctacttttc tattagtagg tgtgtttttg
     2161 tcaaaagcaa aagtccaatc tttatcggca aagtctgcag gcacaggcat ttcttcgtaa
     2221 tcactgttgg cttctaatgg cgtatagcca ggcggaattt ggttccaatc gccgccgacg
     2281 attacatagt ttcctttggc atattcttct acgatgtact ttttaaaata tttcatttct
     2341 tgttttttca attggccacc atcgtaagca gaattatgtg tattgatgac gatgagttgt
     2401 tttccatttt tcaaattaaa atgatgtttt acaaaacatc tatctaaaaa aaacaaacct
     2461 ttaggccaag aaaaattgct tggaaatgca tattttattg gtgttgctgc tgctttatat
     2521 ttataaagtg tggcaatacc acctagtact tttcccatcg gatttgtaaa tggaataggc
     2581 acatatttca catcgtaatt tttaccaaaa gctgcggaat agttgcctaa taaatcaaac
     2641 acttctttta actgatcacg atgataacta cggcgcgaat ctctatctat ttcttggatt
     2701 tcaataaaat cggcatcgtg tttccaacta ttgatggtag ataaaattcc atcaaaattt
     2761 ttatctgtca atgcttttgt agatcgcacc atattgccgc catcataaaa aaaatcagat
     2821 tctttgccta agccagcata accaatattc caattataaa acacaagtgt atctttatct
     2881 atcaatggca aaggctcctt cgcttgtaat acttcggctt gctcttctgc tttcggttta
     2941 taatcggtaa attgcgcata caaaatcgct acaattacat acacaccaaa taaaattcct
     3001 aagccaataa ttagttttaa taagagcacc attctagatt tcatatttta gatttttaat
     3061 gacaatttca ataatttagg tctatcattt acataaaata aaaacgataa gactatgaaa
     3121 tttagctaaa tttagactta ttttacaaac taaaaaaata gaaatatgaa aaatacagtg
     3181 ttgcaatggc aatccaatga tggtgtaaaa atttttggtc aacaatggga aaatgacaca
     3241 cagccagcaa aagcagtgat ttgtttagta catggttttg gcgaacacag ttcgcgatac
     3301 gaacatgtag cacaattttt tacagacaat caatatgctg ttatcactta tgatgcgcgt
     3361 ggtcacggaa aatctgaagg taagcgcggt cacttcgcta gctatgatga atttttaaac
     3421 gatgtaaata atctattgaa acaagcagat atacattttc caaatctgcc tataataata
     3481 tatggtcaca gcatgggtgg acaagttgta gctaactttg ccattaaaca tcaacctaaa
     3541 gttgcaggca ttattctttc ctcgccattc ttccaaccag gctttgcacc gccagcctta
     3601 aaaattgctg ctggcaaaat aatgcgcaat cttatacctt cattttcatt gccatctggc
     3661 ttagatgtaa atgccatttc cagagacaaa gaagttgtaa aaaaatacag caatgaccct
     3721 ttggtattcg atagtatttc ttcaaaaatg ggcattgagc tgatagaatt tggcgcggaa
     3781 gctgtacaaa atgcagcgaa actcagcttg cctaccttac ttttccatgg tactgccgac
     3841 caattgacct catataatga cagtaagaaa tttgccgcca atgctggtaa gaatgtcaaa
     3901 tttatcacct atgatggatt ctatcacgaa tgtcataatg aaccagagaa acaagtggtt
     3961 ttacaaaata tcttagattg gtgcaataaa ttaattggat aatgtacaaa tttcaatttt
     4021 aaacattaca tttgtttaac tatagtattt tttagccttc ttgtcaatgt tataaatgtt
     4081 catttgattc gattataaaa agtatatagc ctaattttta atttaactta ataaaaaaaa
     4141 tgaacatttt tgttgcaaac attgaaagaa aagtgactga cgaacaatta caagacttgt
     4201 tttctcagca cggagaaatt gcttctctta aattaatcag agacagagac acgggcgtaa
     4261 gcaaaggcta tgcgtttgta gaaatgccaa atgatgatga ggctcaaaaa gctatcgatg
     4321 ctttaaatgg ctatgaaatc gacggcagag ctttagcagt aaacgaggct cgcccaaaaa
     4381 cagagtacaa aaaatctggt ggcggcggtg gcggctacaa aggtggcggc ggtggcggct
     4441 acaataaagg tggctacggt ggtggcggtg gctacaacaa aggtggctac ggcggtggcg
     4501 atggcggtta caaaaaaaga tactaatctg aaaaactcat atcaagaacc ttcgagcaat
     4561 cgaaggtttt tttatttaca aaaatgaaca acacgaagca ttgtatattt ttcttgattg
     4621 tataatttag cactgtaata tcattcaaat cattttatga ctgctcattc cttcttagat
     4681 aaattattaa cgagattaaa aagtggtgat gcacgaagta ttcatctaaa tgccttacca
     4741 ggtaattttg caaggttgga tatttatgat ttgataaata tagaatccag tttacatttg
     4801 aaatttttat cgactttgct aagtaaaaga gatttcaaat tttctattac catcgatgct
     4861 acatcagaaa aaaacaaaac agccgaagag aaaaaaatag ttcaaaaaat tataaagcgc
     4921 ctcaaccatc tcgattatca agaaaaagaa gaatttgctg aacatggatt cctttcattt
     4981 ggatttggct atccgctttt aataaaaaga gatcctaata atgaagacag aattataaaa
     5041 gcgccaatac tcatctggta tttgtctata gaaaaagata cacgtagaaa caacacttgg
     5101 acgatttcac gtaacgaaga acatccttta ctttttaatg aattattgca agctcatttt
     5161 gaaaataatg aaaaaataaa aacagatgag ctggaagaat taattgaaga tgattttata
     5221 gatgaaacac aattgttgca gttttgtaaa agccttttag ataaactcaa tattcctttt
     5281 gatactgttg aaacaattgc aacactcgtg cctgcaagca acaaagaaac cattgacaat
     5341 aataccaaag ctgcgccatg gattcggtgg agcggtattt taggtttatt taaaatgcaa
     5401 aagcaaagca tcattaaaga tgtagaattg ctgatgcaat atgaaaatat tatcgattcc
     5461 gaaaacaaca attattttga tggtgaaata ttgtcgcctg tcactttaga tcctagtcag
     5521 gaaaatgttt tacaacaaat aaaacagcat gacaacatta ttatccaagg accaccaggc
     5581 actggcaaaa gccaatcttt gacagctatt attacacaag cattattaaa ccaacaaaaa
     5641 gttttagtag tatgcgaaaa acgtactgct atggaagtgt tgtataataa tttaaaaaaa
     5701 gaaaatttag aacatctttg tgtgcttatc gaagatgtat atagcgatag aaaaacaatt
     5761 gtagagcgtg tgcgtttgca tatcgaaaat gccgagcacg acgacttacg atttagaagt
     5821 aatgaatatg aattagcacg ttctaaattc ttaacgttac aagaagaaat caatcacaga
     5881 ataaacttta gcaataacgt catctttggt gatgataatt ggttggaatt attgcaaaaa
     5941 ggcaaagaat taaaccaaaa taaatcgcat agtgaaaaag caaatgcact cataaaatta
     6001 attcgcaata gcgattatgt gtttacttat aatgagttta tcgaactctc taaaatatta
     6061 aaaaatgctg ctgagctata tcagctgctg agcaatgaag cctttatatt cgatgcaatt
     6121 caagatgata attttgaaaa aattgacaat agcgaaaatt taaaaactag aattttacaa
     6181 ttgcaacagg atattaattc attatcaact agcataacaa acaacattca gttgtataac
     6241 aatgactatg atgtacaaac tggatttacc gcttttaagg taaaattgct ttcttacttc
     6301 agcaataagt ataaaaacat aatacaacaa aaagaaaata ccaaagcaag cctttatcaa
     6361 ctcatacaac aatttaatca gtacgatttt attccaatag atttatcaat tgatgttgca
     6421 aatgaaaaaa ttagtaatgt cattcctgca atccaaaata ttcaaatcac ttgtgatgac
     6481 tatgtgcaac aaatacagca tttgccagct tattttgcgt ttagaaaatt caaactatcg
     6541 caaaaaacgg caacgcaaaa tttaatacag gctttgattc aaataaaatg cgatgaatgg
     6601 tgtgcagtct ttgaaacata ctacatcaat caagcaattt taaaaattgc aatggaaaat
     6661 ggcatcaaag atgactcaca aaaactatat gaagaattgg tgctagctga tatacattgt
     6721 aaacaaaaac tcgcagaaaa aataaatcac acttggaaaa taatgcagca agaaagcatc
     6781 aaaactaaag accttactgc cataaaatat ttatacaatc aaagaaaaaa caaacaattc
     6841 tcctctaaaa attcattgcg caaaattgtg catcaagatt ttgatttttt tacaacttta
     6901 tttcctgtag taatggtcaa tccaagcgtg tgtacttcta ttttaccatt acaaaagaat
     6961 cttttcgact ttattgtttt agatgaagcc agtcaattga gagtggaaga tacttactca
     7021 tctttgttac gtggcaagac gaaaataatt tctggtgata aacatcaaat gccaccaagc
     7081 aattttttcg gaagcgaaac catcttttgg aataaggaag aagaagacga tgcagattca
     7141 tatttagcag aaagcaaatc attattagaa tatgccgaag atgccaattt taaaagcaca
     7201 tatttggatt accattaccg ttctttgcat ccaagtttaa ttcaattttc taatcatgcc
     7261 ttttatcaat ctcgcttaat tccattgcca gaaaaagaaa attatcaggc aatacatttc
     7321 acatctgtag atggtattta taaagatggc actaatatcg cagaagcaaa tgccattgtt
     7381 gatttggttt tttctttaga atcatggaaa gataatttcc catctgttgg cattgcaacg
     7441 tttaatattt ttcaacgtga tttgatatat gatttattgt ttgaacaagc ttataaagac
     7501 aatgcaaaaa atgaaaaatt gcaagcgctc ttaaaccaag gtttatttgt gaaaaattta
     7561 gaaaacattc aaggtgatga gcgcgatgtg atgattttat caagtacttt tgggaaagat
     7621 gaacaaggaa aattcagaca atttttcggt ccgcttacac aagaaaaagg ctatcaattg
     7681 ctcaatgtaa ttattacacg tgcaaaacat tctttacatg tttatacttc agttccagaa
     7741 actacatttt cattatttga agaagaatta gccataaaag gaaataaagg aaaaagtatt
     7801 ttctatgcct atttatctta tgtgaaagct tgtgtagata aaaatcaaac gcaaatcgat
     7861 tacattaaat cttgcttgca aaaaaataat ccaccgatgc ttgtccataa aaaacaacgc
     7921 acttccattt tgaaagaaag cgtatttaat gccttacaaa atgaatttgg tgaaaacgta
     7981 cagctcaact ttgcactcgg tggtttcgtg ttagacatcg tacttttaaa agacaataaa
     8041 ccctatttac atatcgactt tgaaaacaca aatctatacc accacgaagt tacctatcgc
     8101 ataaaattgc atcagcaaaa aatagtggaa caataccata taaaaacata tcatctttgg
     8161 tcatataatt ggtggaatgc cgctgagaac gaaattaaaa acataagtga ttgctactta
     8221 agcaattgat atttgtcaag aaataacatt ccttctcggt aaaacaatga tttctatact
     8281 tttataccta tatttggtta gccttaagca actaattata taccaattat gaaaaaacat
     8341 ttaactagaa gaaagttctt gaaactaact tctgtagcag gtgtagctgc atctttgcca
     8401 ctaagtggat gtaaaaaaga gcctataaaa gttgatgatg tagatatcga agctatcgtt
     8461 gtaggttctg gatttggtgc tgctgtttcc gctttgcgct taacacaagc tggtgtaaaa
     8521 acgttaatgt tggaaatggg aaagcaatac gatgtggatg ctactccaaa tccatttact
     8581 aaaacattat cgccagatgg cagatcgaca tggttaaaac gcagaactat tttaccggta
     8641 acttctatcc aatttgatat tcctaaatat gttggtgtgc ttgaccgtgt tgaatacaac
     8701 acctcatata tgcgtatttt tagaggcact tgtttaggtg gtggttctgt agtatatggt
     8761 gcgatgttgc catacgcgca accttccatg tggaacgaat attttccata tgtatcctac
     8821 gatgacatgt tgaataaatg gtatccaaag gtgaaaaata tattagattt ttctgtaatt
     8881 cctgatgata ttctgaacag caaatattat aaatatgcgc gagttggttt agagcattgc
     8941 gaaaacgcag gcatggaaaa agtaatgtta aatgctggcg ttaacttcaa taccatcaga
     9001 agtgaaatcg atggtactac tcaaaaatgc tcaattgata atcaattatt atatggcgcc
     9061 aataatggat ttaaaaatag cttggacaga aattatatac cacaagcaaa tgcaacagga
     9121 aatctaacta ttgaaacatt acacaaagta gattctgtta gacaattaag caataaaaat
     9181 tatgaactca cagttcataa aatcaatgaa caaggcgaaa cagtagccgt aaaaatttat
     9241 acttgtaaat atttattcat caatgctggt gtagtgggca cgatgaatat tttactaaaa
     9301 tcaaaacact taggtggctt ggctaattta agcgatg
//
LOCUS       DAOQQV010000104         9330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2766_length_9330_cov_5.949434, whole genome shotgun sequence.
ACCESSION   DAOQQV010000104 DAOQQV010000000
VERSION     DAOQQV010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2766_length_9330_cov_5.949434"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..230)
                     /locus_tag="PLL59_09915"
     CDS             complement(60..230)
                     /locus_tag="PLL59_09915"
                     /inference="COORDINATES: protein motif:HMM:NF025570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HMY24165.1"
                     /translation="METFKIEIQEFLSRIVEIEAENIDDAVLKVKEMYRQEEIVLSEN
                     DYITTEIEEYIE"
     gene            complement(217..996)
                     /locus_tag="PLL59_09920"
     CDS             complement(217..996)
                     /locus_tag="PLL59_09920"
                     /inference="COORDINATES: protein motif:HMM:NF017822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HMY24166.1"
                     /translation="MNLAFNTNLAHGYTSNSQIARLLTENWVLNNSYCPSCGDLPLIE
                     FENNRPVADFYCKKCSEEFELKSKSGKLSNTITDGAYSTMIERINSENNPNFFFLTYT
                     KNWTVNDFLIIPKQFFTAEIIIKRPPLAETARRAGWIGCNIDISKVAEAGKVFLIKNT
                     QIINQNFVKETFKKTLFLRTKSKDAKGWILDIMSCVDLIKKETFTLDEVYKFEQKLKL
                     KYPNNNFIKDKIRQQLQLLRDKGIIEFVGRGNYKKVNYGNI"
     gene            1576..2541
                     /locus_tag="PLL59_09925"
     CDS             1576..2541
                     /locus_tag="PLL59_09925"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HMY24167.1"
                     /translation="MHLKFQQKVVIPDLKLISGRCTQYLSSTIAECYGQDLTPVEVMQ
                     FSDGEFEPVITESIRGSYVFCMQSTFAPADNLMELLLLLDAAKRASAGYVVAVMPYFG
                     LARQDRKDKPRVAIGAKLVANMLVAAGAQRVMTMDLHSQQIQGFFDIPVDHLEADAIF
                     IPYLKELSLEDLVFASPDVGGIKRARNYARQFNAELVICDKHRAKANEIESMRLIGDV
                     TGKNVILVDDLIDTGGTLTAAANLLMEKGAKSVRALITHPVLSGKAYERIEESALTEL
                     VVCDTIPLRQQSTKIKVLSVAPLFARAIRNAHEFKSINSLFIQKK"
     gene            2547..3146
                     /locus_tag="PLL59_09930"
     CDS             2547..3146
                     /locus_tag="PLL59_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HMY24168.1"
                     /translation="MQTITISGKARAGVGKVATKADRAAGNIPCVMYAGNEVVHFTST
                     ISELRGILYTPKFYKAIINIDGTTHEALLKDVQVHPITEEVRHIDFHKLIPGTPVIVE
                     IPIRVVGQSPGVKEGGKLLVKVRKVKVKSTPENLKDNIDVDISELKLGQSFKVRDIKE
                     TGFEILNSPAIPLVSVEITRALKSAATEAAKAESSSKKK"
     gene            3276..>6124
                     /locus_tag="PLL59_09935"
     CDS             3276..>6124
                     /locus_tag="PLL59_09935"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HMY24169.1"
                     /translation="MKKNFFVLFYFVISNVFSNTLWQKISSENIKIEGIRKIIPQKYN
                     LLQLNNAAFQLFQQSIPKEQSRQSVIIDLPTPEGDFIKFKIFELPCMDPILAAKFPTI
                     KTYQALCVDNPAITAKLDYTSFGFHAMVFSPKGVYFIDPFSTSNTGYYTCYYKKDYSK
                     ISNSNTACQTGIIENTLAHSSQRITQTTNGIVNSIATGVRRNYRLALACTREYSIAVC
                     APSAPTKTLVLSAMITTLNRVNGVYEKDLSVHMDLIANNDTLIFIGTDNYSNDDGGAM
                     LDQNQTVCDARIGNANYDIGHVFSTGGGGVAYLASVCDNQIKAKGVTGSSEPIGDGFD
                     IDYVAHEMGHQFGANHTFNGNTGACNGNRNSTTAYEPGSGITIMGYAGICDANDIQAH
                     SDDFFHRASIVEIFNHISSTSCAMISNTGNQAPNVLSYKSTYYIPYKTSFEVTATASD
                     PNGFPLNYLWEEWDLGPAGSWSNANIITAPILRSFSPTPSPTRTFPRWDSLINNVIRY
                     KGEVLPEVARDVKLRCTVKNIDPNGYGAFNAPDSNITVKSIATPTLFRVTSHAAPSII
                     TGNTTITVNWDIANTTAAPISCANVQIYLSLDSARTFPYLLATTSNDGSEVINIPDVV
                     TDNASARIKVKGAGNIFFDLNNGWIKINQGVPIVQASFSASDSVICQGENILFSNTTT
                     GNPDSVKWTIHGGLPDTSTSLTSFNAQFDSSGIYMVSLVAYKNGNASNVFVKNITVHP
                     NPLIVFSPTSPNICSGDSIALSVAYIAGATCIWSNLSTNDTISVAPTQDTYYSISITN
                     NECTSVDSVLVKVNPTKTTNIANTICSDDSVVIGNQVFKTTGIHSVVMQTSQGCDSTV
                     VLDLTVHSTKTTNIANTICSDDSVVIGNQVFKTTGTHSVVMQTSQGCDSTVVLDLTVH
                     STKTTNIANTICSDDSVVIGNQVFKTTGIHSVVMQT"
     assembly_gap    6125..6224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6225..7014
                     /locus_tag="PLL59_09940"
     CDS             <6225..7014
                     /locus_tag="PLL59_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY24170.1"
                     /translation="TNIASTICSDDSVIIGNQVFKTTGIHSVVMQTSQGCDSTVVLDL
                     TVHSTKTTNIASTICSDDSVIIGNQIFKTSGTHSVVMQTAQGCDSTIVLDLTVHDVPI
                     QAVITQSHDTLFCNVIMAGATYNWLQSNNIDTLTNEPYLIIRNSGAYAVQVHSVDNCT
                     SDTSDYFNAILTAIKSTKTSTSFSVSPNPNNGSFLVYISSVKSALLQLKLYSITGQIL
                     KQETISTLQGNTIFPINMQGIEKGMYFISLYNEDGVATQNIIIQ"
     gene            7070..7636
                     /gene="pth"
                     /locus_tag="PLL59_09945"
     CDS             7070..7636
                     /gene="pth"
                     /locus_tag="PLL59_09945"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HMY24171.1"
                     /translation="MSKYLIIGLGNIGSDYAHTRHNIGFDVVDKLAENLSTTFDISRL
                     AAIAKTTYKGKQIHIIKPTTYMNLSGKAIKYWMNELSIPIENIFVIVDDLALPFGQLR
                     VRSKGSDAGHNGLKSIQEELQSAAYPRLKFGIGNDFAKGKQVEFVLGKWKPEQEVILQ
                     ERINIAVEIIKSFCVAGLSNTMNGFNNK"
     gene            7665..8270
                     /locus_tag="PLL59_09950"
     CDS             7665..8270
                     /locus_tag="PLL59_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMY24172.1"
                     /translation="MKIFCVGLNYKGHIVEMKHFAFPDNPVIFMKPPTALLKNGQAFF
                     HPEFSEDVHYEGEIVLRVCKNGKKIDKKFAHKYYDAFTIGFDFTARDIQAEQKKKGWP
                     WEIAKGFDGSAFIGDFIPLQQGQLISFTIKKNNQIVQQGDTSMLIFSFDEIISYISKF
                     FTLQQGDLIYTGTPQGVGAVKIGDVLEGFVDNNAIVRCEIK"
     gene            8384..>9330
                     /gene="metK"
                     /locus_tag="PLL59_09955"
     CDS             8384..>9330
                     /gene="metK"
                     /locus_tag="PLL59_09955"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HMY24173.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAYDAQSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQDIAREVIRKIGYTKSEYMFEANSCGIFSAIHEQSSDI
                     NQGVDRKSKKEDFETKANAQGAGDQGMMFGYATKETDNYMPLALDIAHKILQELSKTR
                     RAGKELKYLRPDAKSQVTIEYNDDNQPIRIDTIVVSTQHDDFDSDAKMLAKIKKDIIE
                     VIIPRVKKQLKPAIQKLFNDKITYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGA
                     HGGGAFSGKDPSKVDRSAAYATRHLAKNLVAAGVCDEVLV"
BASE COUNT         3139 a         1560 c         1625 g         2906 t
ORIGIN      
        1 caaatttatg ttcaaattca ggcaagaggc tcattgtatc tccgtgaaga agatagaagt
       61 cactctatat attcttcaat ttcagtggtt atataatcgt tttcggacaa aacaatttct
      121 tcttgtcgat acatttcttt tacttttaaa actgcatcat caatattttc tgcttctatt
      181 tctactattc ttgatagaaa ttcttgaatt tcaattttaa atgtttccat aatttacttt
      241 tttataatta ccacgaccaa cgaattcaat aattccttta tctcgaagta gttgtaattg
      301 ttgtctgatt ttatctttga taaaattatt atttggatat tttaatttca gtttttgttc
      361 aaatttgtaa acttcatcaa gtgtaaaagt ttcttttttg attaaatcca cacaactcat
      421 aatatctaaa atccaacctt tggcgtcttt actttttgtt cgtaaaaaca gagttttttt
      481 gaatgtttcc tttacaaaat tttggtttat aatttgagtg tttttaatca aaaagacttt
      541 ccctgcttct gcaacttttg aaatatcaat attgcaacca atccaacccg ctcttcttgc
      601 tgtttcagct aatggcggtc ttttaataat aatttcagct gtaaaaaatt gtttcggaat
      661 tatcagaaaa tcattgacag tccaattttt tgtgtaagtc aaaaagaaaa aattaggatt
      721 gttttctgaa tttatccgtt caatcattgt cgaatatgca ccatctgtaa ttgtatttga
      781 aagttttccg cttttgcttt ttagttcaaa ttcttcgcta cattttttac aataaaaatc
      841 tgcaacagga cgattatttt caaattcgat taatggcaaa tctccgcaac tcggacaata
      901 tgaattgttt aaaacccaat tttccgttaa taatctcgca atttgagaat tgctcgtata
      961 tccgtgtgct aaatttgtat taaaggccaa attcatatta tgcgactgct tgctttatgt
     1021 ttataggtaa tgccttttca atatcgttaa tgagctaagt ttttcctcct gcccatttta
     1081 aaaaaggttt tgcaaatcta tttttagcca ttttaatatt ttcgggttct tcaaaattac
     1141 aattataatt ttatttaatt tctgtatgtt ttataaaata gtagattgtt gaaatcttta
     1201 tgttcgatta atattagatt ttagtgattt taatttcaat taatctacaa actgttgtcg
     1261 agattaaata aatcaaaagt tgataataca acaaaattgt ggattatttc tatatgtgta
     1321 aaagcgtagc gcagtctcac cttcggtgtg actagttaag tgtggttatt atcgttgtgc
     1381 gaaattatct acttgttttc ttttgcctta gagaagaagt tgaatggttt gaccttgaca
     1441 agcagttgaa taataaatac atatctgcat caaaccgaat acatattatt gcatttctcc
     1501 atttcagatt aaaaaattac aaaatccgcg attcttaacc ttaattctcc aaatttttgt
     1561 atttaattac tatctttgca cctcaaattt caacaaaaag tggtaattcc tgacttaaaa
     1621 ctgatttctg gcagatgtac acagtacctg tcttctacaa ttgctgaatg ttatggtcag
     1681 gatttaacgc cagtggaagt catgcagttc agtgatggtg aatttgagcc tgtaatcaca
     1741 gaaagtattc gaggttctta tgttttttgc atgcaaagta cctttgcacc agccgataat
     1801 ttaatggaat tgctattatt gttagatgct gccaaaagag catctgctgg ttatgtcgta
     1861 gcagtaatgc catactttgg cttggctcgc caagacagaa aagacaaacc acgtgtggca
     1921 ataggcgcaa agttggtcgc caatatgctg gttgccgctg gtgctcaacg cgtgatgacg
     1981 atggacttgc attcacaaca aatacaaggt ttttttgata ttccagttga ccatttagaa
     2041 gcagatgcta tttttattcc atacttgaaa gagcttagtt tagaggattt agtatttgct
     2101 tcgccagatg ttggtggcat caaacgtgcc agaaattatg cacgccaatt taatgcagaa
     2161 ttggttatct gcgataaaca cagagcaaaa gccaatgaaa tcgaatctat gcgtcttatc
     2221 ggcgatgtta caggtaaaaa cgtaatattg gtagatgacc taattgatac tggcggtaca
     2281 ttgacagctg ctgctaatct tctcatggaa aaaggagcca aaagtgttcg tgctttaatt
     2341 actcatccag tattatcagg taaagcatac gaaagaattg aagagtcagc actgacagaa
     2401 ttagtggtat gtgataccat tcctttaaga caacaaagta caaaaataaa agtgctatcg
     2461 gtagcgccat tgtttgcaag agctatccga aacgcacacg agtttaaatc tattaattca
     2521 ttattcattc aaaaaaagta agtattatgc aaaccattac cattagcgga aaagctagag
     2581 caggcgtagg aaaagtagca acaaaagcag atagagctgc tggcaacatt ccttgcgtga
     2641 tgtatgcggg aaacgaggtg gtacacttca catcaactat ctctgagttg agaggaatct
     2701 tgtacacgcc aaaattttac aaagcaatca tcaacatcga tggcactaca cacgaagcct
     2761 tgttgaaaga tgtacaagta catccaatta cagaagaagt tagacacatt gacttccaca
     2821 aattaattcc aggcactcct gtaattgtag aaattcctat cagagtggta ggtcaatctc
     2881 caggtgtaaa agaaggtggt aaattattgg tgaaagttcg taaagtgaaa gtaaaatcaa
     2941 cacctgaaaa tctaaaagac aatatagatg ttgatatcag cgaattgaaa ttaggccaat
     3001 ctttcaaagt acgtgatatt aaagaaacag gctttgaaat cttaaactct cctgcaattc
     3061 ctttggtatc tgtagagata acaagagcat taaaatctgc tgctacagaa gctgcaaaag
     3121 cagaatcttc tagcaagaaa aaataagctc ctttatataa caaaaagacc ggaattttcc
     3181 ggtctttttt tgttttagca aacaccatat ttgacacaga tgtgtgacta tatgctatcg
     3241 aatttctatc tttatacata ataattgttc gtctaatgaa aaaaaacttt tttgtcttgt
     3301 tttattttgt gattagcaat gtgttctcaa atacattgtg gcaaaagata tcttctgaaa
     3361 atattaagat agaaggcatc cggaaaatta ttcctcagaa atataatcta ttgcaattaa
     3421 ataatgctgc attccaatta tttcagcagt caatacctaa agaacaaagt aggcaatcgg
     3481 taattattga tttgccaaca ccagaaggcg attttataaa gtttaaaata tttgaacttc
     3541 cttgtatgga tccgatactg gctgcaaaat ttccaacgat aaaaacgtat caagctttgt
     3601 gtgttgataa tcctgccatc acagcaaagc tagactacac atcatttggt ttccatgcta
     3661 tggttttttc tcctaaaggt gtttatttca ttgacccatt ttcaacaagc aatactggtt
     3721 attatacctg ttattataaa aaagattatt caaaaataag caattcaaat actgcttgtc
     3781 aaacaggtat catcgaaaat acactcgctc attcttctca aagaatcaca caaacgacga
     3841 atggaattgt caatagtatt gcaacaggtg ttaggcgaaa ttatcgcttg gctttagcat
     3901 gtacaagaga atattccatt gcagtgtgcg caccgagtgc accaacaaaa acattagtgt
     3961 taagtgcaat gattacaacg ctaaatcgtg taaatggtgt gtatgaaaaa gacctttctg
     4021 tgcacatgga tttaatcgcc aataatgata ctttaatttt tatcggtact gataattata
     4081 gcaatgatga tggcggtgct atgttagatc aaaatcaaac agtatgcgat gcacgcatcg
     4141 gaaatgctaa ctatgatatt ggacatgtat ttagtacagg tggtggtggt gtcgcatatt
     4201 tggctagcgt ttgcgataat caaattaaag ccaaaggcgt tactggttca tcagagccta
     4261 taggcgatgg atttgatatt gattatgttg cgcatgaaat gggacatcag tttggcgcaa
     4321 accatacatt taacggcaat acaggtgcat gtaatggcaa cagaaatagc actacggcgt
     4381 atgaacctgg tagtggcatt actattatgg gttatgctgg aatttgcgat gcaaatgata
     4441 ttcaagctca ttcagatgat tttttccata gagcaagtat tgtagaaata tttaatcata
     4501 tttcgtccac atcttgtgcg atgataagca acacgggtaa tcaagcacct aacgtgctaa
     4561 gctataaatc tacttattat attccgtata aaacaagctt tgaagtgaca gcaactgcaa
     4621 gcgatccgaa tggctttccc ttgaattatc tttgggaaga atgggattta ggtcctgctg
     4681 gttcatggag taatgctaat attattacgg caccaattct tcgttctttc tcgccaactc
     4741 catctccaac aagaacgttt cctcgttggg atagcttaat taataatgtg attagataca
     4801 aaggtgaagt gttgccagaa gtggcacgcg atgttaagct tagatgtact gtcaaaaata
     4861 tcgatccaaa tggatatggt gcattcaacg cgcctgatag caatattact gtaaaatcaa
     4921 tagcaactcc taccttattt agagtaacaa gtcatgcagc accatctatt attacgggca
     4981 atacaacaat aacagtaaat tgggatattg ccaacacaac agcagctccg atttcatgtg
     5041 caaatgttca aatctatttg tcacttgaca gcgctagaac atttccttat cttctagcga
     5101 ctacttcaaa tgatggtagt gaagttatta atattcctga cgtcgtcacg gataatgcat
     5161 cagcacgtat taaagtaaaa ggtgctggaa atattttctt tgacttaaat aacggttgga
     5221 ttaaaatcaa ccaaggtgta cctattgtgc aagcatcttt ttctgcaagc gattctgtaa
     5281 tttgccaagg tgagaatata ctatttagca acacaactac aggtaatcct gattctgtaa
     5341 aatggactat tcatggaggt cttcctgata cttcaacctc gttaacttct ttcaatgctc
     5401 aatttgattc cagtggaatt tatatggtaa gtttagttgc atataaaaat ggaaatgcaa
     5461 gtaatgtttt tgtaaaaaat atcacagtac atccaaatcc tttaattgta ttttctccaa
     5521 catcaccaaa tatttgttct ggtgattcaa tagcgctaag cgttgcttat atagcaggtg
     5581 ctacttgcat ttggagtaat ctgtcaacaa atgacaccat atcagtagca ccaactcaag
     5641 atacatacta ttcaataagc attacaaata atgagtgcac ttctgtagat tctgttttag
     5701 taaaagtcaa tcctaccaaa acaacaaata ttgcaaacac aatatgcagt gatgatagtg
     5761 tagtaatcgg caatcaagta ttcaaaacaa caggcataca cagcgtagtc atgcaaacat
     5821 cacaaggttg cgacagtaca gtagtattag atttaacggt gcattcgacc aaaacaacaa
     5881 atattgcaaa cacaatatgc agtgatgata gtgtagtaat cggcaatcaa gtattcaaaa
     5941 caacaggcac acacagcgta gtcatgcaaa catcacaagg ttgcgacagt acagtagtat
     6001 tagatttaac ggtgcattcg accaaaacaa caaatattgc aaacacaata tgcagtgatg
     6061 atagtgtagt aatcggcaat caagtattca aaacaacagg catacacagc gtagtcatgc
     6121 aaacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacaaa tatagcgagc
     6241 acaatatgca gcgatgatag tgttataatc ggcaatcaag tattcaaaac aacaggcata
     6301 cacagcgtag tcatgcaaac atcacaaggt tgcgacagta cagtagtatt agatttaacg
     6361 gtgcattcca ccaaaacaac aaatatagcg agcacaatat gcagcgatga tagtgttata
     6421 atcggcaatc aaatattcaa aacatcaggt acacacagcg tagtcatgca aacagcacaa
     6481 ggttgcgata gcactatagt attagattta acggtgcatg atgtgccaat acaagctgta
     6541 attacacaga gccacgatac tttgttttgc aatgtgatta tggctggtgc aacgtataat
     6601 tggcttcaat caaacaatat tgatacactt acaaatgagc cttatttaat aattagaaat
     6661 agtggagctt atgcggtgca agtccatagt gttgacaatt gcacatcaga tacttccgat
     6721 tattttaatg cgattttaac agcaataaaa tctacgaaaa cttcaacatc atttagtgta
     6781 tctccaaatc cgaataatgg cagttttcta gtgtatattt catctgtgaa atctgcatta
     6841 cttcaactga agctatacag tattacaggt caaatactca aacaagaaac gatttcaact
     6901 ttacaaggaa atactatctt tccaataaat atgcaaggca tcgaaaaagg aatgtatttt
     6961 atttccttgt ataatgaaga tggtgttgca acacaaaata taattattca ataatccaaa
     7021 aatagatagc tttgctgttg atgtctatta cacaagtgct tgttttttta tgtcaaaata
     7081 tttaataata ggtttaggga atattggaag tgattatgca cacactcgtc ataatattgg
     7141 atttgacgtg gttgacaaat tagcagaaaa tttatctaca acttttgata tttccagatt
     7201 agctgctatc gcaaaaacta catataaggg caaacaaata catatcataa agcctacgac
     7261 ttatatgaat ctaagtggta aagctattaa atactggatg aatgaattaa gcattcccat
     7321 tgaaaatatt tttgtaattg ttgacgattt agccttgcca tttggtcaat tgcgtgtgcg
     7381 ttctaaaggc agtgatgcag gccataatgg tttaaaatct attcaagagg aattacagag
     7441 tgctgcctat ccacgattaa aatttggcat tggaaatgat tttgccaaag ggaaacaagt
     7501 agaatttgtt ttgggaaaat ggaaaccaga gcaagaagta attttgcaag aacgaattaa
     7561 cattgcagta gaaattatta aatcattctg cgtagctggc ttgtcgaata ctatgaacgg
     7621 ttttaataat aaataatttt ttctcccaaa tatccaatac gaacatgaaa atattttgcg
     7681 tcggactgaa ctataaagga catattgttg aaatgaagca tttcgcattt cctgacaatc
     7741 ctgtcatctt tatgaaaccg ccaacagcac tcttgaaaaa tggacaagct tttttccatc
     7801 ctgaattttc tgaagatgta cattatgaag gagaaattgt attgcgcgtt tgtaaaaatg
     7861 gaaaaaaaat agataaaaaa tttgcacata aatactacga tgcattcacc atagggtttg
     7921 actttacagc acgcgatatt caagcagaac aaaagaaaaa aggctggcct tgggaaattg
     7981 caaaaggttt tgatggttcc gcttttatag gtgattttat accacttcaa caaggtcaac
     8041 taatatcatt tacaattaaa aaaaacaatc aaattgtgca acaaggcgat acgtccatgc
     8101 ttattttttc gtttgatgaa ataataagtt atatatctaa atttttcacc ctacaacaag
     8161 gcgatttaat ttacacgggt acgcctcaag gtgttggtgc tgtaaaaatt ggtgatgtat
     8221 tagaaggttt cgtagataat aatgcaattg ttcgttgtga aatcaaatag tctatctaaa
     8281 tcgcagttaa ttagtgcaac aaattttgtt ttcaatagaa ctttaattat ttttgaagtc
     8341 taattttaaa ttttttattc atcatcaata aacataataa taaatgcctt atttatttac
     8401 gtcagaatca gtatcagaag gtcaccctga taaagtagca gaccaaattt cagatgcttt
     8461 aattgataat tttttagcgt atgatgcgca atcaaaagta gcatgcgaaa cactagttac
     8521 cacaggtcaa gtggttttag caggtgaagt aaagtctaaa gcatatttag acgtgcaaga
     8581 cattgctcgt gaggttatca gaaaaattgg ttataccaaa tcagaataca tgttcgaagc
     8641 taattcttgc ggtatttttt ctgctattca cgaacaatct tccgacatca accaaggcgt
     8701 tgacagaaaa tctaaaaaag aagatttcga aaccaaggca aatgcacaag gtgctggcga
     8761 ccaaggtatg atgtttggtt atgcaacaaa agaaacagat aactatatgc cattggcttt
     8821 agatattgca cacaaaatct tacaagaatt atcaaaaaca cgtagagcag gaaaggagtt
     8881 gaagtattta cgtcctgacg caaaaagtca agtaactatc gagtataacg acgataacca
     8941 acctatccgc attgatacaa ttgttgtttc tacacaacat gacgattttg attctgatgc
     9001 gaaaatgtta gcaaaaatca aaaaagatat aattgaagtt attattcctc gagtaaaaaa
     9061 acaattaaaa ccagccattc aaaaattatt caacgataaa attacttacc atatcaatcc
     9121 aacaggaaag tttgtaattg gtggcccaca tggtgatact ggtttaacag gaagaaaaat
     9181 tatcgtggat acttatggtg gtaaaggtgc acacggcggt ggagcatttt caggtaaaga
     9241 tccaagtaaa gtggacagaa gtgcagcgta tgctactcgc catctagcaa aaaacttagt
     9301 agctgcaggt gtttgtgacg aagtattagt
//
LOCUS       DAOQQV010000105         9304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2775_length_9304_cov_7.788950, whole genome shotgun sequence.
ACCESSION   DAOQQV010000105 DAOQQV010000000
VERSION     DAOQQV010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_9304_cov_7.788950"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PLL59_09960"
     CDS             <1..1041
                     /locus_tag="PLL59_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HMY24174.1"
                     /translation="EFGFICSTENENLQVTRNPWNINYTTGGSSSGSAALVASGVVPI
                     ATANDGAGSTRIPASCCGLVGLKPTRNRLYYMQGTETLPVNIAYQGVLTRSVRDTAAF
                     YAAAEQFHHHKKLPAIGHVQTPLNRMLRIAYFDNLPEGKQGHMDEDTFRVQLENAKLL
                     ESLGHSVEPIKVPLDVEEMTKYYLTYYGFLAYMSINFGRLSQGAKVDKSLVLDPFTLG
                     LADYFSNRKLKLIGNLRALVNAAKRTEAELQQKYDVIMTAVTSKRTPEIGYFSPLLSY
                     EEMAWRGSDLAAFLPLFNISGSPAISLPLGVASNDMPVGVQFVAPYGQDKLLLELALQ
                     LEQANPWKLICD"
     gene            complement(1045..1470)
                     /locus_tag="PLL59_09965"
     CDS             complement(1045..1470)
                     /locus_tag="PLL59_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24175.1"
                     /translation="MKIGFLSCFILLSTFTALAQADFMAMNNNIVERNQALHIHKAYF
                     GQENQNTEKELKRAKRLKIAGLVFTSLGTAGILTTTPFAVIYSSVSKNTHGWGNVAAA
                     VVSMMGYIPSGACLISGIPMTVIGFKKANKLKASTTKEF"
     gene            complement(1539..2198)
                     /gene="gldD"
                     /locus_tag="PLL59_09970"
     CDS             complement(1539..2198)
                     /gene="gldD"
                     /locus_tag="PLL59_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HMY24176.1"
                     /translation="MSIRINSYMNTLSHRYITTLFHRFIIIFCSVIIITTFTACRENY
                     TPKQSGYFRIDFPKNRTFVTYENTSCPFSFDYPSYGKIQKDSSNAEELNNHPCWFNII
                     FPEYKAKIYLSYTAISAANPLDKLIRDSYKLTFKHTVKADYIDETIITGKNKNVSGIM
                     YDVGGNAASAVQFYLTDSAHHFLRGSLYFYASPNVDSIAPAVAYFRKDVEHIVESLQW
                     K"
     gene            complement(2264..3625)
                     /gene="gldE"
                     /locus_tag="PLL59_09975"
     CDS             complement(2264..3625)
                     /gene="gldE"
                     /locus_tag="PLL59_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HMY24177.1"
                     /translation="MDDGIPIPFLLQAILGTSTSEVFNTFNATVAIELLFTLILLIFT
                     AMMCAVEVAIFLFTTQEINELERSNDETDAIILDFIKKPRRFLATFVLTTTLFTLAVV
                     LMFENVLETILKHEFIEANSSLLLIIKILFETFIIVLFAEVIPKLYATQQYYKVAKNT
                     VHLLRIFDILFSPFVRALLATSNFFEKRLAGFSNNVTAKDIDEAIDITTSQENDEAIR
                     NDSRILKGIVKFGNIVVTQIMHSRMDVVAVDKNATFEELLSIVRESGYSRIPVVEENF
                     DKVAGIIYIKDLLRYIDAPKDFNWQQLIKPAMFVPESKKIDDLLEDFQAKRVHMAIVV
                     DEYGGSSGIVTLEDVLEEVIGDIKDEFDDASEIDFKKIDNYNYIFEGRTALNDVCKVL
                     EVPTTTFDDARGDADSLAGLVLELNAEIPVEGDVLTFKHFTFLILEADKTRVIRVKVT
                     IAN"
     gene            complement(3647..4081)
                     /locus_tag="PLL59_09980"
     CDS             complement(3647..4081)
                     /locus_tag="PLL59_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMY24178.1"
                     /translation="MNKVILIGRLGKDPEVRKINATTTVCNFPLATNESYKNQDGSYT
                     EQTEWHNIVMWRGVAERAERILKKGNTIFVEGKLRTRSWEDKENHKRYTTEIVVENFQ
                     LLDKREPGATTNTANNEQKTDTESNVATDNNSEPTMDDDLPF"
     gene            complement(4151..5194)
                     /gene="mutY"
                     /locus_tag="PLL59_09985"
     CDS             complement(4151..5194)
                     /gene="mutY"
                     /locus_tag="PLL59_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HMY24179.1"
                     /translation="MSTFSKKLLQWYNPNERNLPWKQTNDAFKIWLSEIILQQTRVAQ
                     GTPYYLAFIKKYPTISKLAKAPLDEILKLWEGLGYYSRARNLHQASIQIMEKHNGHFP
                     NTYPEILQLKGVGKYTAAAIASFAYGLPYAVVDGNVYRVLARVFGIEIPIDSTLGKKH
                     FEEIAQQLLDKDNAALYNQAIMDFGALVCKPSNPLCNQCPFSSSCIALTQDKIQHLPV
                     KTKKITKTTRHFHYFVIHNTAHILIQQRMQNDIWHGLFEFPMIETKDHTLPNDTKSIF
                     GSNKKLSTTSSFSFKQELTHQTIRAHFHEIKLSSLPRQNTDLIHAVKRDLTKYVFPKI
                     IHSYLQVRLIYLT"
     gene            5411..5716
                     /locus_tag="PLL59_09990"
     CDS             5411..5716
                     /locus_tag="PLL59_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMY24180.1"
                     /translation="MRKADIITTISEKTGVPKVDVLVAMEAFFKEVKDTLADGENLYV
                     RGFGSFIVKTRKAKIGRNIKKNIAIEIPEHQIPAFKPSKVFVEQIKESSENRLKNKE"
     gene            5727..6566
                     /locus_tag="PLL59_09995"
     CDS             5727..6566
                     /locus_tag="PLL59_09995"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY24181.1"
                     /translation="MQKGQIVLGLGALILVLALYFGGKTKEKKESNTTHAVSAGLSFE
                     QYEQSQLEKIEATAKEEYSSLLSAIRNTGKADTAKLHHDNMALASFWHRQNNPALHAY
                     YYYQAMSANKSKQTLESVADTLVYAYKTTEDSVILNNLITFALRSYEEAVKQDTSDVD
                     LRLKLAEAYIQGSQEPMKGVAILKELENKHPNDINILMSLGRLSIQSGQLDKAKERLK
                     KILTLEPQNREAIYFLAITEAQLGHTEEAIRLFELCKLMVNNEDFNKEIDEIVKNLKS
                     KKV"
     gene            6720..8261
                     /locus_tag="PLL59_10000"
     CDS             6720..8261
                     /locus_tag="PLL59_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HMY24182.1"
                     /translation="MIKELVIHANSKGVEIALLENKKLVEYHLDSYDKEGFSAGDIYL
                     GRVKKINPGLNAAFIDIGHDKDAFIHYSDLSPYIRSVRKFSSEAFKAEQSHLLDKFEF
                     EPPIKKDGLMSDALEKGDILVFQISKEAISTKGPRLTCELSIPGRYVVLVPFTNSIGI
                     SKKIDKPEERERLLQTMQKIKPKNFGVVVRTNAAGVGQDELSYDIQNLMNKWKVMTDN
                     LKYQNPPKLLLKEMNKTFSIVRDLMNDSFSKIITDNPSLHGDLKAYIKEYAPEQTQIL
                     SIHEDRTPLFETYEVSRQIKTAFGKTVTLKSGAYVILEHTEALHVIDVNSGPKVKKDV
                     SQDTLAFNINVEAAQEIARQLRLRDIGGIIVVDFIDMKSSEYKHKLYEAMQQAMKPDK
                     AKHVILPVSKFGLMEITRQRMKEQVVIDTREPLLHGNSKYKVDQPLQIIDNIETELHH
                     LKSHKDKNFLLYVHPFIYAFMKKDTFSFRMRWYSKIGKIVRLIQDSSLHLGEYKLLTK
                     EGEKA"
     gene            complement(8336..8944)
                     /locus_tag="PLL59_10005"
     CDS             complement(8336..8944)
                     /locus_tag="PLL59_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24183.1"
                     /translation="MKTQFKFRLIILAFATISAFSIGCTPTGPSLPLPCSTVNTLFQQ
                     IYTNVVAIPGDTNTVTMDTEVHGYDFTVSANKTICKIGYQSQPAVASQLYKIEIIDNT
                     TSTTLFSQNTTFSATTTSYISVPNIAVVPGHSYTIQRTLTNWLGNIGNTVGRLVREVN
                     GGSIPFPHTVGVMTITGARFHQTSNPSVVSTFGIPYIDIAFQ"
     gene            complement(8984..>9304)
                     /locus_tag="PLL59_10010"
     CDS             complement(8984..>9304)
                     /locus_tag="PLL59_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24184.1"
                     /translation="TDLMSQDEILQLFEPIIPGEYTFESGEIKVEDGQACLVLRTRGK
                     ATLTEFTIRCDLVNPENEGGLALASGEYTLSSSDASSSPSTHPIPSLASKKKTYVGHV
                     TLLK"
BASE COUNT         2994 a         1590 c         1754 g         2966 t
ORIGIN      
        1 gaatttggat ttatttgtag tacagaaaat gaaaacttac aggttactcg taatccttgg
       61 aatataaatt atacaactgg tggctcatca tcgggctctg cagcattagt ggccagtggt
      121 gtggtgccta ttgcaacggc taatgatggc gctggctcaa cgcgaattcc tgcttcgtgt
      181 tgtggtttag taggtctaaa gcccacgcgc aatagattgt attatatgca aggcacggag
      241 acattgccag taaacattgc ttaccaaggt gttttgacac gtagtgtgcg cgatactgca
      301 gcattttatg ctgctgcaga gcaattccat caccacaaaa aattgccagc aattggacat
      361 gttcaaacac cattaaacag aatgttgcgc attgcttatt ttgataattt accagaagga
      421 aaacaaggac acatggacga agataccttt cgtgtgcaat tggaaaatgc aaaattgtta
      481 gagtcattag gccattcggt agaaccgatt aaagtgccac tagatgtgga agaaatgacg
      541 aaatactatc tgacttatta cggtttttta gcttatatgt ctattaattt tggtcgattg
      601 tcacaaggcg caaaagtgga caaatcattg gtgttagatc cttttacatt aggcttagct
      661 gattattttt ccaacagaaa attaaaactc attggcaatc taagagcatt agtcaacgcc
      721 gcaaaacgca cagaagctga gttgcaacaa aaatatgatg ttatcatgac tgctgttact
      781 tcgaaacgca cacctgaaat aggctatttt tcgccattgc ttagctacga ggaaatggca
      841 tggcgtggtt cggatttggc agcattttta ccattgttta atatctctgg ttcgcccgca
      901 atatcactgc ctttaggtgt tgcttcaaat gatatgcctg taggcgtgca atttgtagca
      961 ccttatggtc aagataaatt attattagag ttggctttgc aattggaaca agcgaatcct
     1021 tggaagttga tttgtgatta gtacctaaaa ttcttttgtc gtacttgctt ttagtttgtt
     1081 tgctttttta aagccaataa cggtcattgg tatgcctgaa attaaacaag cgccacttgg
     1141 aatgtaaccc atcatgctca ctacggcagc agccacattt ccccagccgt gcgtgttttt
     1201 actaacactt gaataaataa ctgcaaatgg cgttgttgtt aaaattccag ctgtgcctaa
     1261 gctagtaaat actaatcctg caatttttaa acgctttgct cgttttaatt ctttttcagt
     1321 attctgattt tcttgtccga aataagcttt gtgaatatga agtgcttgat tgcgctcgac
     1381 tatattgtta ttcatcgcca taaaatctgc ttgcgcgagt gctgtaaaag tagatagcaa
     1441 aataaagcac gaaaggaaac ctattttcat gaaactgtat tttattgttt aaatatacag
     1501 tatgtttttt acaattattc caataatttc agatgatatt atttccattg aagtgactct
     1561 acaatgtgtt ctacatcttt tctaaaataa gcgacagcag gtgcaataga atcgacattt
     1621 ggtgatgcat agaaatacaa agagccacgt aaaaaatggt gtgcactgtc tgtaaggtaa
     1681 aactgcacag cactggcagc attaccacca acatcataca tgatgccact tacattttta
     1741 ttttttcctg taataatggt ttcgtcgata tagtcagcct ttactgtgtg tttgaatgtg
     1801 agcttgtaac tatctcttat gagtttgtct aatggattgg cagcgctaat ggcagtatag
     1861 cttaaataaa tttttgcttt atactcagga aaaataatat tgaaccaaca aggatgattg
     1921 ttgagctctt ctgcattaga agagtctttt tgtatttttc catagcttgg ataatcaaat
     1981 gaaaatggac atgatgtatt ttcataagtt acgaaagttc gattctttgg aaagtcaatt
     2041 ctgaaatagc cactttgttt aggcgtgtaa ttttcgcggc aagcagtaaa tgttgtaata
     2101 ataatgacac tacagaatat aataatgaaa cgatgaaata atgtagtaat atatcgatgt
     2161 gacaatgtgt tcatgtagga attaattctt atgctcataa ataagtattt gtaatagcta
     2221 tttttatttt ctttgtggat tgctgtttta tggaataata tcattagtta gcaatcgtta
     2281 ctttcactct aataactctt gttttgtcag cttctaaaat taaaaatgta aaatgcttaa
     2341 aggtgagcac atcaccttcg acaggaattt cagcatttaa ttctaagact aaaccagcta
     2401 aagagtcagc atcacctcgt gcatcatcaa aagtagttgt aggcacttcc aatactttac
     2461 aaacatcgtt caatgctgtt cggccttcaa atatataatt gtaattatct attttcttaa
     2521 aatcaatttc gctggcatca tcaaattcat ctttgatatc gccaatgact tcttccaaaa
     2581 cgtcttctaa agttacgatg cctgagctac caccgtactc atcaacaaca attgccatgt
     2641 gcacacgttt cgcttggaag tcttccagca agtcgtctat cttttttgat tccggcacaa
     2701 acatagcagg ttttatgagt tgctgccaat taaaatcttt cggtgcatca atataacgca
     2761 ataaatcttt gatgtaaata ataccggcta ctttgtcaaa attttcttct acaactggaa
     2821 tacgcgagta gccagattct ctcacaatac ttaataattc ttcaaaggtg gcatttttat
     2881 ctacagcaac aacatccatg cgcgagtgca tgatttgtgt gaccacgata ttgccaaatt
     2941 ttacaatacc ttttaaaatt cgcgaatcat ttctaatggc ttcgtcattt tcttgcgatg
     3001 ttgtaatgtc tatcgcttcg tcaatatctt ttgctgtaac attgttagaa aaaccggcta
     3061 atcttttttc aaaaaaatta gacgtagcca ataatgctct cacaaaaggt gaaaataaaa
     3121 tatcgaatat tctaagtaaa tgcacggtgt ttttcgctac tttatagtat tgttgtgtag
     3181 catataattt cggaattact tcggcaaata atacgataat gaaggtttca aataatattt
     3241 ttataatcaa caataatgag ctattcgctt cgataaattc gtgtttcaat atcgtttcca
     3301 atacattctc aaacatcaaa acaacggcaa gtgtaaataa agtagtcgtc agtacaaacg
     3361 ttgccaaaaa ccgtcgaggt tttttgataa aatctaaaat aatggcatct gtctcatcat
     3421 tacttcgttc aagctcattt atttcttgcg ttgtaaaaag aaaaatagcc acttcgacag
     3481 cacacatcat ggcggtgaaa atgagcagta ttaaagtgaa aagtagctca atggctacgg
     3541 tcgcattaaa tgtattgaat acttcacttg tggaagtgcc gagtatagct tgtaataaaa
     3601 agggtatggg tatgccgtcg tccaaagtat tgtaatttct tatctgttaa aatggcaaat
     3661 cgtcgtccat tgtcggttct gaattgttgt ctgtcgcaac attcgattcc gtatctgttt
     3721 tttgttcgtt gtttgccgta tttgttgttg cgccaggctc tcttttatct aaaagttgga
     3781 agttttcaac cactatttct gttgtatagc gtttgtgatt ttctttgtct tcccaagagc
     3841 gtgttctcaa ctttccttcc acaaatatag tatttccttt ctttaaaata cgttctgcac
     3901 gctcagcaac accacgccac atgacaatgt tatgccattc cgtttgctct gtatagctac
     3961 catcttggtt tttgtatgac tcgttagtgg ctaatgggaa attacaaaca gtagtcgttg
     4021 cgtttatttt tctcacttct ggatctttgc ctaaacgacc tattaagatt actttgttca
     4081 cactcatggt ttcgatttta catgtaaaaa aaagctactt tttgacaaaa gtcaagtatt
     4141 attattttaa ctaagttaaa taaattaatc gaacctgcaa atagctgtgt attatttttg
     4201 ggaatacata ctttgttaaa tctcgtttta cagcgtgaat taaatcggtg ttttgcctag
     4261 gtaaactgct taattttatt tcgtggaaat gcgcacgaat cgtctggtgt gtaagttctt
     4321 gtttgaagga aaaacttgat gtagtactta gtttcttatt gcttccaaaa atagatttgg
     4381 tgtcatttgg caatgtatgg tcttttgttt caatcatcgg aaattcaaat aagccatgcc
     4441 aaatgtcatt ctgcatgcgt tgttgtatga gtatatgtgc cgtattatgt atgacgaaat
     4501 aatggaagtg ccttgttgtt tttgttattt ttttcgtctt gacaggcaaa tgttgtattt
     4561 tatcttgtgt caaagcaata catgatgagc taaatggaca ttgattacat aaaggatttg
     4621 atggtttgca aactaatgca ccaaaatcca taatggcttg gttgtataaa gctgcattgt
     4681 ctttgtcgag taattgttga gcaatttctt caaaatgctt cttgccaagt gtgctgtcaa
     4741 taggaatttc aattccaaaa acacgtgcca aaacacgata tacattgcca tctacaacag
     4801 cgtaaggcaa gccataagca aacgaagcaa tggcggcagc ggtgtacttg ccaacacctt
     4861 ttagttgtaa tatttcagga tacgtattcg gaaaatgacc attgtgcttt tccatgattt
     4921 gtatggaagc ctgatgtaaa tttcgtgctc ttgaataata acctaagcct tcccaaagtt
     4981 tcaagatttc atctaatggt gcttttgcca atttgcttat ggtcggatac ttttttataa
     5041 atgccaaata gtaaggagtg ccttgcgcta cacgagtctg ctgtagaata atttctgaca
     5101 accaaatttt gaacgcatca ttagtttgtt tccacggtaa atttctttca ttcggattat
     5161 accattgcag taatttttta gaaaaagtac tcattgattt gcaaaataag atttattgaa
     5221 aatagtaccg cattattatc agaaatatta ttagatgtaa ctttcagatg ttagataagt
     5281 ctcataaaaa taaatacaaa taacagtatt aatttttcgt aacttacaca ttttcttatt
     5341 gatatttcgt gtatattagt gagttagttg gaaataattt tgcatttttg tacttcatta
     5401 atcgataagc atgagaaaag cagatatcat caccacaatt tcagagaaaa caggtgtacc
     5461 taaagtagat gtattagtag ccatggaggc tttcttcaaa gaagtaaaag acacattggc
     5521 tgatggtgaa aatttgtatg tacgtggctt tggaagtttt atcgtgaaaa cacgtaaagc
     5581 gaaaattggt agaaatatca agaaaaatat tgccatagaa attcctgaac atcaaattcc
     5641 ggcattcaaa ccatcaaaag tattcgtgga gcaaataaaa gaatccagcg aaaaccgttt
     5701 aaaaaataaa gaataagact taaaacgtgc agaagggaca gatagtatta ggattaggtg
     5761 cacttatact cgtattggcg ctatactttg gcggaaaaac caaagagaag aaagaaagta
     5821 acacaacgca tgccgtatca gcaggactca gttttgaaca atacgaacaa agtcagctcg
     5881 aaaaaataga agctacagca aaagaagaat atagttcctt gctttctgcc attagaaata
     5941 ctgggaaggc agatactgca aaattgcacc acgacaatat ggctttagca agtttctggc
     6001 atcgccaaaa caatcctgct ttacatgctt attactatta tcaggcaatg tccgccaata
     6061 agagtaaaca gacattggaa agtgtagcag acacgctggt ttatgcatat aaaacaacag
     6121 aagactcagt aattttaaat aatttgatta cctttgcgct ccgctcttac gaagaagccg
     6181 tgaagcaaga taccagcgat gttgatttgc gcttgaaatt ggcagaagca tatattcaag
     6241 gctcacaaga accaatgaaa ggtgtggcta ttctcaaaga gttggaaaac aagcatccaa
     6301 atgatatcaa tattttgatg tctttaggca ggctttctat ccaatctgga cagctggata
     6361 aagctaaaga gcgcttgaag aaaatattaa cgttagagcc acaaaataga gaagccatct
     6421 atttcttggc gataacagaa gcacaacttg gtcatacgga agaggcaata cgcttgtttg
     6481 agttatgcaa gttgatggtc aacaacgaag attttaataa agaaatagac gaaatcgtca
     6541 aaaatttaaa aagtaaaaaa gtttaattat gccttgcgga aaaaaaagaa agcgtcataa
     6601 aattgcgacg cacaaacgta aaaaaagact aagaaaaaac cgtcataaga aaaagtaatt
     6661 agtaaatagt agtaagtgat aagtttctta ttacttgcta cttaaaactt acacctaata
     6721 tgataaaaga attagttata catgctaatt ccaaaggtgt tgagattgct cttcttgaga
     6781 ataaaaaatt agtcgaatac catcttgatt catacgataa agaaggattt tcagccggtg
     6841 atatctactt aggtagagta aaaaaaatta atccgggatt gaatgctgcc tttattgata
     6901 ttggtcatga caaagatgca tttatacact attcagattt aagcccatat attcgttctg
     6961 ttcgtaaatt tagttcagaa gcatttaagg ctgagcaatc acacttattg gataaatttg
     7021 aatttgagcc acctataaaa aaagatggac taatgtctga cgccttagaa aaaggcgata
     7081 ttttagtatt tcagatttct aaagaagcaa tttctactaa aggaccacga ttaacctgcg
     7141 agctgtcgat tcctggaaga tatgttgtgt tggtgccatt taccaactcc attggcattt
     7201 ctaaaaaaat agataagcca gaagaacgcg aacgcttgct gcagacgatg caaaaaatta
     7261 aaccaaaaaa ttttggtgta gtcgtcagaa caaatgccgc tggcgtaggt caagatgaat
     7321 tgagctacga tattcaaaat ctgatgaata agtggaaagt gatgacagat aatttgaagt
     7381 atcaaaatcc gccaaaactc ctattgaaag agatgaataa aaccttctct atcgtcagag
     7441 atttgatgaa tgatagcttc tcaaaaatca ttacagataa tccatctttg cacggcgatt
     7501 tgaaggcata tatcaaagaa tatgcgccag agcaaacaca aatactttct atccacgaag
     7561 atagaacgcc attgttcgaa acgtatgaag tgagccgaca gattaaaact gcgtttggta
     7621 aaacggtgac tttaaaaagc ggtgcttatg tcattttaga acatacggaa gcattgcatg
     7681 tcatcgatgt gaatagtgga ccaaaagtga aaaaagatgt ttcgcaagac actttagcgt
     7741 ttaatataaa tgtggaggca gcgcaggaaa tagcacgtca attgcgcttg cgtgatattg
     7801 gcggaattat tgttgtcgat tttattgaca tgaaatcatc agagtacaaa cataaactgt
     7861 acgaagccat gcaacaagcc atgaagccag ataaagccaa acatgttatc ttgcctgtga
     7921 gcaaatttgg cttaatggaa attacgcgtc aacgcatgaa agaacaagtc gttattgaca
     7981 cacgcgaacc acttttgcat ggcaatagca agtataaagt ggaccagcca ttacaaataa
     8041 ttgacaatat cgaaacggaa ctgcatcatc taaaatcaca caaggacaaa aacttcttgt
     8101 tgtatgtgca cccgtttatt tatgcattta tgaagaaaga tactttttca tttagaatgc
     8161 gatggtattc taaaatcgga aaaattgttc gcttaattca agattcttcg ttgcacttag
     8221 gagaatataa attgctgacg aaagaaggcg agaaggctta gtttaggttt caataataat
     8281 atgaaaacat cgaagctgaa tagtagcttc gatgtttttt ttattgtcaa aaaaattatt
     8341 gaaaagcaat gtcaatataa ggtatgccaa atgtagaaac aacagatgga ttgctagttt
     8401 ggtgaaaacg agcacctgta atggtcatca caccaacagt gtgtggaaat ggaatacttc
     8461 cgccattcac ttctctcact aatctgccaa ccgtattgcc gatattgccg agccagttgg
     8521 tcaatgttcg ttgaatcgtg tagctatgtc ctggtacaac agcaatgttt ggaacagaaa
     8581 tatagctggt agttgtggcg ctgaatgtcg tattttgact aaacaaagta gtgcttgtcg
     8641 tgttgtcgat tatttctatt ttgtatagct gtgaagcaac cgcaggctga ctttgataac
     8701 ctattttgca aattgtttta ttggctgaca ccgtaaaatc ataaccatgc acttccgtgt
     8761 ccattgttac ggtgtttgtg tcgccaggaa tggccaccac attcgtatag atttgttgga
     8821 ataaggtatt gacagtgctg caaggcaatg gcaagctggg accagttggc gtgcaaccaa
     8881 tgctaaatgc ggaaatagtg gcaaaagcta agatgattaa tcggaattta aattgtgttt
     8941 tcattgtaat tattttaaag atgaagattt ggatttattt ttattatttc aaaagcgtta
     9001 catggccaac gtatgttttt ttcttgctgg caagtgatgg aattggatgt gtagatggcg
     9061 agcttgaagc atcactggaa gacaatgtat attcgccaga tgctaatgct aatccgcctt
     9121 cgttctctgg atttactaaa tcacatctaa ttgtaaattc tgtcaaagtt gctttgcctc
     9181 ttgttcttaa tactaagcat gcttgaccat cttctacttt tatttcacca ctttcaaaag
     9241 tgtattcacc aggaataatt ggttcaaaaa gttgaagaat ttcatcttgt gacataagat
     9301 ctgt
//
LOCUS       DAOQQV010000106         9121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2864_length_9121_cov_7.531988, whole genome shotgun sequence.
ACCESSION   DAOQQV010000106 DAOQQV010000000
VERSION     DAOQQV010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2864_length_9121_cov_7.531988"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLL59_10015"
     CDS             complement(<1..474)
                     /locus_tag="PLL59_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HMY24185.1"
                     /translation="MLKTTTYQVINKIPCHFFDKHLLALLFVLFSISASFAQRFTAQA
                     EYSQVPLNYTVDVAYAVEGGKIESFSPPKFDGFEVYGPSQGTNISIINGKISKSSSIT
                     YTLKPKKQGNYTIPPASAIVNGKEMKSNAVSISVVAAQEQRRGNDPFADMMEEMEE"
     gene            complement(493..1302)
                     /locus_tag="PLL59_10020"
     CDS             complement(493..1302)
                     /locus_tag="PLL59_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY24186.1"
                     /translation="MKNKIAQIKLFIQKHKTLFIVLAVLKVLLKVGIVLFFVAKANGA
                     VAQNANKLLREGNKLYKKEKYNNATESYAKALQLAHTDVRAYFNQGDALYRLNELDKS
                     KEQFTAVTKASKNTDITARAHYNIGNIHYKQEKWEESAKAYKESLKLNPKDEDAKYNL
                     MMALAKIKKEKNNNKDNKNNKDDKNKEDKNDNKDKDKQQNQQQNKNDKQQQSQDKQQD
                     QNKQQQQQQGQISKEQAEKLLDAIGAEEGKVQQKLGKEKGKPQKGKIQKDW"
     gene            complement(1292..2317)
                     /locus_tag="PLL59_10025"
     CDS             complement(1292..2317)
                     /locus_tag="PLL59_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMY24187.1"
                     /translation="MIRFEHTEYLLLLLLIPLAIVVYFYVQIQTKRQLSAIGNIALIK
                     RLMPERNDRKSLIKFVLLNLIFCLLAIALAQPQMGTKMEKVQRKGIDVMIALDVSNSM
                     LSKDIAPSRLEKSKLLIQTLLKKLDGDRVGLVIFAGNAYLQMPLTVDYSSLMLYLRAI
                     NTNSVPTQGTIITEALDKAEGAFTQTDKKHKAIIVITDGEDHEEDAIDKAEEIASDGT
                     KIFTIGAGTPAGGSIPLMDQNGQIVGEKKDENGQTIITKMNANMLQALAKAGNGNYYI
                     LDNTKQVADFIAKDISKMETKTINDKVFADYVEQFQYFLFFALLLLLLDIFTTFRRKM
                     LDFKNEK"
     gene            complement(2342..3349)
                     /locus_tag="PLL59_10030"
     CDS             complement(2342..3349)
                     /locus_tag="PLL59_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMY24188.1"
                     /translation="MTAIFPYTFAHPNYFWLLLLVPALIYWFYYKRSTYFPTFAIPQH
                     GDVLQQASTLRVRLLQFLPALKITAFILLVTALARPQSSLSESEISTQGIDIVVSLDI
                     SGSMLAKDFEPDRLEASKKVAMQFIKERKSDRIGLVVFSGESFTQCPITIDHQVLLNL
                     FKDIHSGMVEDGTAIGMGLATAVARLKESKSKSKVIILMTDGVNNMGYIDPYTAIQIA
                     KTYGIRVYTIGVGKNGTAPYPVKDANGNTFYQNMPVQIDEPLLRKIAQETGGYYYRAT
                     NNTSLKKVYENIDKLEKSKIKISNYHRKSERFHLFAFIALFCLLLEFILSKTYLKTIL
                     S"
     gene            complement(3373..4395)
                     /locus_tag="PLL59_10035"
     CDS             complement(3373..4395)
                     /locus_tag="PLL59_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24189.1"
                     /translation="MKSFLQKIQITATTLTSSKKMMLLLFVCSLSSIFNQIIYAQTAT
                     ASVKEKTFLIGDWIPVDLFVSSTANNKVAFPSLKDSITETIEVANYTPIDTIKKSGQY
                     EYHQLVNLIVFDTGNINLPQFQFLVDNNGKIDTILTSPITVRVAGVKIDTTKDIKDIK
                     APLKIPYTFKEILPYIIALLILLIIIGIIVWYIKKRKQKQQPIDEKFLLPPHVWALKE
                     LEKLHHKKLWQTGAVKNYYSELTDIARTYIELRYKIPAMEKTTDELMDSMHKGMMKQS
                     LKKELNDVLILSDFVKFAKAQPDFVENENAFKVVQDFIDKTKQIEEIKKGNDKNINII
                     NKENTK"
     gene            complement(4416..5282)
                     /locus_tag="PLL59_10040"
     CDS             complement(4416..5282)
                     /locus_tag="PLL59_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMY24190.1"
                     /translation="MEVAELIKKVRKIEIKTKGLTNHIFSGEYHSAFKGRGMSFSEVR
                     EYQYGDDIRNIDWNVTARFQHPYVKIFEEERELTIMLLIDVSKSAFIGTQTQLKNEIM
                     AEIAGVLAFSAIQNNDKVGVLFFSDAIEMFIPPKKGKSHILRIIREILNFEPKSNKTN
                     ISLALESFNNMVKRKSIAFVLSDFIDENYANELRHISKKHDVIGVKVYDNLDMELPNI
                     GLLQVQDVETGETKWLDTKDKQTQKAYSERASFYDNIYNDSFVKAGADKLKINTAQAY
                     VHILMNFFKKRA"
     gene            complement(5352..6344)
                     /locus_tag="PLL59_10045"
     CDS             complement(5352..6344)
                     /locus_tag="PLL59_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMY24191.1"
                     /translation="MESFDIKALNEKIQQDSAFLEPLHTEIGKSIIGQKYMLERLMIA
                     LLSQGHILLEGVPGLAKTLAIKSLSDAIDTKFSRIQFTPDLLPADILGTMIYNQAKGS
                     FEVKKGPVFSNFLLADEINRAPAKVQSALLQSMQERMVTLGDTTYKLEEPFLVMATMN
                     PIEQEGTYNLPEAQVDRFMLKVKIGYPEFEEERQIMRQNVAGSNPGIKPIIAAQTILQ
                     ARDTVRQVYMDEKIEKYILDIIFATREPSKYKINDLTHLISYGGSPRASISLAMASKA
                     YAFIKRRGYVIPEDVRAVAHDVLRHRIGITYEAEAENITQEHIIDTLLNRVEVP"
     gene            complement(6495..7523)
                     /locus_tag="PLL59_10050"
     CDS             complement(6495..7523)
                     /locus_tag="PLL59_10050"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HMY24192.1"
                     /translation="MKKLLFMLLALIAMHTTTLAQVEKQIKGEALSADEKKKLEDTTN
                     GWKFGGIGGVTFNQAGFKNWAAGGTNSFSLLFNMRFNADYKKNNHLLQMWAAAEYGLQ
                     FTKGDQYKLRKNADRWEIFTKYGYRIHKPLYFAAYADLRSQFSNSYNYTDTAKYVISR
                     AAAPLIFEGALGLDYVPNKYFSLFFSPLAAKVTYVAANDIAALNSFGNIFPSKVKKEF
                     GAVVIATYKQEFWKQNISILSVFKAYKNYLRSSADPFDATLPKESKSHYRKNIDVDWQ
                     TTLGLKVNKFLSASIFMHLIWDNDVTFPTDNPALKTSKVQFRDIIGVGLTYQMNYTKA
                     KGSAAKKL"
     gene            complement(7679..>9121)
                     /locus_tag="PLL59_10055"
     CDS             complement(7679..>9121)
                     /locus_tag="PLL59_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMY24193.1"
                     /translation="KYPKVGEKNAKLSVHIYQLKNKKTKTVAIPYAYEYIPRINWNKS
                     GTELIMLLMNRNQDTMRLVAYNTSSKKTRQLYLETDTAYVNIPTALTFLNNNSFIITS
                     AKNGYNHLYHYDENGKLLQQITNGNYEIKQLYGIDETKHWIYYQSNEGNEIETAIYRI
                     HLFTHDKEKLSNQNGSNSAIFAKDFSRYLHQFSSATTPPTYSIRNTNDTATIVLVNNQ
                     DIQKKTENIPQKKFIQIPNNDNYLNAWIITPKAIDTTQKYPLFIYTYGGPESQSVENK
                     WSGTRDLFLNFLAEQGYFVACIDNRGTDGRGKKFKQATFKQLGKLETEDQTQAVQYLC
                     NQFPIDKNNVCIYGWSYGGYLAANCLLQPNSIFKTAIAAAPITNWKLYNTIYTERYMR
                     TPTENASAYANFQPEKLAKNLQGNLMLIHGTADDNVQLQHTLRLIQSLNTSNKNYQLY
                     LYPDEAHGLGSSKLYYDLYRKIFNYLKEKN"
BASE COUNT         2535 a         1543 c         1596 g         3447 t
ORIGIN      
        1 ttcttccatt tcttccatca tatcagcaaa cggatcattg cctctgcgtt gttcttgtgc
       61 cgctacaacg ctaatagaaa cagcattcga tttcatctct ttgccattga caatagcgct
      121 cgctggtgga atggtataat tgccttgttt cttaggcttc agcgtatagg tgatgctgct
      181 tgatttagat atttttccgt taatgataga aatatttgtg ccttgtgatg gaccgtacac
      241 ttcaaatcca tcaaattttg gaggcgaaaa gctttcaatt tttccacctt caacggcgta
      301 tgcaacatca acagtataat ttaaaggtac ttggctgtat tcggcttgtg cagtaaagcg
      361 ttgcgcaaat gatgcagaaa tactaaataa aacaaacaat aatgcaagta aatgtttgtc
      421 aaaaaaatga catggtattt tgttgatgac ttgatatgtt gttgttttta gcattggtct
      481 atatacacat ttttaccagt ctttttgaat tttccctttt tgaggttttc ctttttcttt
      541 gcctaatttt tgttgcacct tgccttcttc agcgccaatt gcgtcgagta gtttctctgc
      601 ttgttcttta cttatttgtc cttgttgctg ttgttgctgc ttgttttgat cttgttgttt
      661 gtcttgactt tgttgctgct tgtcgttttt gttttgttgt tggttttgct gcttgtcttt
      721 atctttattg tcatttttat cttctttgtt tttatcgtct ttattgtttt tgttgtcttt
      781 gttgttattc ttttcttttt ttatttttgc cagcgccatc atcaaattat atttagcgtc
      841 ttcatcttta ggatttaatt tcaaagattc cttgtatgct ttagcactct cttcccattt
      901 ttcttgtttg taatggatat ttcctatgtt gtaatgcgca cgcgctgtta tatctgtatt
      961 ttttgaagct ttagttacag cagtaaattg ttctttcgat ttgtctaatt cattcaagcg
     1021 atataatgca tcaccttgat tgaaatatgc gcgcacatca gtatgtgcaa gctgtaaggc
     1081 ttttgcataa ctttcagttg cattattgta tttttctttt ttgtatagct tgttgccttc
     1141 tcttaaaagt ttattcgcat tttgtgctac agcaccattt gcttttgcca caaaaaacaa
     1201 gacaatgcct acttttaaaa gtactttaag cacagccaac acgataaaaa gtgttttgtg
     1261 cttttgaata aataatttta tttgcgcaat cttatttttc atttttaaag tcaagcattt
     1321 ttcttctaaa tgttgtaaaa atatctaaca ataataaaag taaagcaaaa aacaaaaagt
     1381 attgaaactg ttctacataa tcagcaaata ctttatcgtt tatcgttttg gtttccattt
     1441 tcgaaatgtc ttttgctata aaatcagcaa cttgctttgt gttgtctaaa atataataat
     1501 tgccattgcc tgctttcgct aatgcttgca gcatgtttgc attcatttta gtaatgatgg
     1561 tttgtccgtt ttcatctttt ttttcaccta ctatctggcc attttggtcc attaatggaa
     1621 tagaaccacc agcaggcgtt ccggcgccaa ttgtaaatat tttagtgcca tcgctggcaa
     1681 tttcttctgc tttgtcgata gcgtcttctt catggtcttc accatcagta atcacaatta
     1741 ttgccttgtg ttttttatct gtttgtgtaa atgcgccttc cgctttgtct aaagcttctg
     1801 tgataatggt tccttgtgta ggaacagagt tggtattaat agcacgcaaa tacaacatca
     1861 aggaagaata atcaacagtt aaaggcattt gcaaataggc attgccagca aaaataacta
     1921 atccaactct gtcgccatcg agctttttta ataaggtttg aattaataat ttagactttt
     1981 ctaatcttga aggcgcaatg tctttcgaca acatactatt cgatacgtct aacgcaatca
     2041 tgacgtcgat gcctttgcgt tgcacttttt ccattttagt gcccatttgt ggctgcgcta
     2101 atgcgatggc aagtaagcag aaaattagat ttaagagtac aaattttatt aatgattttc
     2161 tgtcattgcg ttctggcatt aatcgtttaa tgagcgcaat attgccaatc gctgacaatt
     2221 gtctttttgt ttgaatttgc acatagaaat acacgactat tgctaatggt attagtagca
     2281 agagcagtaa atattcggta tgttcaaatc gtatcatgaa agtgtgtatc gcgttgtttg
     2341 tttaggataa tattgttttt aaataagttt tagacaaaat aaattctaag agtaaacaga
     2401 ataaagcgat gaaagcaaaa agatggaatc gctcgctttt tctgtgataa ttgctgattt
     2461 ttatttttga tttttctaat ttatcgatat tctcatacac ctttttcaac gaagtattat
     2521 tcgttgctct ataataataa ccaccagttt cctgtgcaat ttttcttaac agcggttcgt
     2581 caatctgtac cggcatattt tgataaaaag tattgccatt cgcatctttc acaggatatg
     2641 gtgcagttcc atttttacct acaccaatag tatatacgcg aatgccataa gtttttgcaa
     2701 tttgaatggc ggtataaggg tcgatatatc ccatattatt gacaccatct gtcatgagta
     2761 taatcacttt gcttttagat ttactttcct tcaaacgtgc aacagcagtg gccaatccca
     2821 tgccgatagc agtgccgtct tcgaccatgc cgctatgtat gtctttaaat aaatttaaaa
     2881 gtacttgatg gtcgattgta attggacatt gcgtaaagct ttcacctgaa aatacgacta
     2941 atccgattct gtcgcttttt ctttctttta taaattgcat ggctactttc ttagaggctt
     3001 ctaatcggtc tggttcaaag tctttagcga gcattgagcc tgaaatatct aatgaaacta
     3061 caatgtcaat accttgtgta gagatttcac tttctgacag tgagctttga ggacgcgcca
     3121 atgccgtcac taacaatata aatgcagtaa ttttcaaagc aggcaagaat tgcaagagtc
     3181 tcactctaag tgtagaagcc tgttgcaaaa cgtcgccatg ttgtggaata gcgaatgttg
     3241 gaaaataggt actgcgctta tagtaaaacc aataaattaa agcaggcaca agcaacaaaa
     3301 gccaaaaata atttggatga gcaaatgtat atggaaatat agcggtcatg gattgcttct
     3361 tatactttga ttttacttgg tattttcttt gttgatgata ttaatatttt tatcgtttcc
     3421 tttttttatt tcttctatct gttttgtttt gtcgataaaa tcttgcacta ctttaaaggc
     3481 attttcattt tctacaaaat ctggttgtgc ctttgcgaat tttacaaaat cacttaatat
     3541 taatacatcg ttgagttctt ttttcaacga ttgtttcatc atgcctttat gcattgaatc
     3601 catcaattcg tcggtggttt tttccatcgc cggaattttg tatcgcagtt ctatgtatgt
     3661 acgagcaata tctgtcaatt cggaataata atttttaaca gcaccggttt gccaaagttt
     3721 tttatgatgt agtttttcca attccttcaa tgcccaaaca tgaggaggca gtaaaaattt
     3781 ttcatcaatg ggttgttgct tttgctttct ttttttgata taccaaacga taattccaat
     3841 aattattaat aatatcaaca gagcgatgat gtatggtaat atttccttga atgtatatgg
     3901 aatttttaaa ggcgctttaa tgtctttgat gtcttttgta gtatctattt taacgcctgc
     3961 tacacgtaca gttataggag atgtgagtat ggtgtcaatt ttcccattgt tatctactaa
     4021 aaattgaaat tgtggcaagt tgatatttcc tgtgtcaaat acaattaagt ttaccagctg
     4081 atggtattca tattgtccac tttttttaat ggtgtcaata ggtgtatagt tggccacttc
     4141 tatggtttca gtaatagaat cttttaaact tggaaatgcc actttgttat tggccgttga
     4201 tgatacaaat aagtcaacag gaatccagtc tccgattaaa aaagtttttt cttttactga
     4261 agcagtagca gtttgcgcat aaataatttg attgaaaatt gaagaaagtg aacatacaaa
     4321 taacaacagc atcatttttt tgcttgatgt aagtgttgtt gctgtaattt gtattttttg
     4381 taaaaatgat ttcatcaata attaattctt cagtattatg ctcttttctt aaaaaagttc
     4441 atcaaaatat gtacataagc ttgtgctgta tttattttta atttatcagc tccagcttta
     4501 acaaagctat cgttgtagat attgtcgtaa aaagaagcac gctcgctata tgctttttgt
     4561 gtttgtttgt cttttgtgtc cagccacttt gtttcgccag tttcaacgtc ttgcacttgc
     4621 aataggccta tgtttggtaa ttccatgtct aaattgtcat acacttttac gccgataaca
     4681 tcatgttttt tagagatgtg tcgcaattca tttgcatagt tttcatcaat aaaatcggat
     4741 agaacaaaag cgatgctttt tcgttttacc atattgttga agctttccag tgctaatgaa
     4801 atattggttt tattgctctt aggttcgaag tttaaaattt cacgaatgat gcgtaaaata
     4861 tgagatttgc cttttttagg aggaataaac atttctatgg catcgctaaa aaacaaaacg
     4921 ccaactttat cattattctg tatggcagaa aatgctaaaa cacctgctat ttctgccata
     4981 atctcatttt ttaattgtgt ttgtgtgcca ataaatgctg atttgctgac atctattaaa
     5041 agcattattg tcaattctct ttcttcctca aaaatcttta cataaggatg ttggaaacga
     5101 gctgttacat tccaatcgat attgcgaata tcatcgccat attgatattc acggacttca
     5161 ctaaaagaca taccgcgtcc tttgaacgca gaatgatact caccagaaaa gatatggttc
     5221 gttaatcctt tagttttaat ttcaattttc cttacttttt ttattagctc tgctacttcc
     5281 atttcataat gattaaagat ttgaaatagg agatttttct cgtgatttct gcctctatta
     5341 catttttact gttaaggaac ttccacacga ttcaataagg tgtcgatgat gtgttcttgt
     5401 gtaatgtttt ctgcttctgc ctcatacgtg atgccgatgc gatggcgcaa tacatcatgt
     5461 gctacggcgc gcacgtcttc aggaatgaca taaccacgtc gcttgataaa tgcataggct
     5521 tttgaagcca ttgccaagct aatgcttgca cgtggcgaac caccatagct gattaaatgc
     5581 gtgaggtcat tgattttgta tttcgaaggt tcacgtgttg cgaatatgat atcaagaata
     5641 tacttttcta ttttttcatc catatatact tggcgcacag tgtcacgtgc ttgcaatata
     5701 gtttgcgctg caatgatagg tttaattcct ggatttgaac cagccacatt ttggcgcatg
     5761 atttgtcttt cttcttcaaa ttcaggatag ccgattttta cttttaacat aaaacggtca
     5821 acttgcgctt ctggtaagtt gtatgtgccc tcttgttcaa ttggattcat ggtcgccatg
     5881 actaagaatg gttcctccaa tttataagtc gtgtcgccaa gcgtcaccat tctttcttgc
     5941 atcgattgca ataatgcact ttgcactttt gctggtgcgc gattgatttc atcagccaat
     6001 aagaaattag aaaaaacagg tccttttttt acttcaaaac tacctttagc ttgattgtat
     6061 atcatggttc ctaagatatc ggctggcaat aaatcaggcg tgaattgaat gcgcgaaaac
     6121 tttgtgtcga tagcatctga caatgattta atagctaaag tctttgctaa gcctggcaca
     6181 ccttctaaca agatatgacc ttgcgaaagc agcgcaatca tcagtcgttc tagcatgtat
     6241 ttttgaccta tgatagattt gccgatttca gtatgtaaag gttctaagaa agcactgtct
     6301 tgttgtattt tttcatttaa tgctttaatg tcaaaagatt ccatgagtat tgtttttata
     6361 gcgttacaaa aatagaatcc ttatcaaatt tctattttga tatattgtta aataatgtta
     6421 gcaattaagg ttatttagca aaaataaacc acagtgaaat ggctgtggtt tattggaatt
     6481 aatttagtaa aattttacag ttttttagct gcacttcctt ttgctttcgt ataattcatt
     6541 tggtatgtca atccaacgcc gatgatgtct ctaaattgaa cttttgatgt ttttaatgct
     6601 ggattgtctg tcgggaaagt tacatcatta tcccagatta aatgcataaa tatgcttgca
     6661 gacaagaatt tattgacctt caatcctaat gtagtttgcc aatcaacgtc tatatttttt
     6721 ctataatgcg atttgctttc ttttggaagc gttgcatcaa aagggtcagc acttgagcgt
     6781 aagtagtttt tatatgcttt aaacacagat aagatgctga tgttttgttt ccagaactct
     6841 tgcttataag tagcaattac aacagcacca aattcttttt tcactttaga aggaaaaata
     6901 tttccgaacg aatttaatgc agcgatgtcg tttgcagcga catacgttac ttttgctgct
     6961 aatggagaaa agaataagga gaaatattta ttaggcacat agtctaaacc taatgcacct
     7021 tcgaaaatca aaggagcagc agctcgtgag atgacatatt ttgcggtgtc tgtatagttg
     7081 tatgaattag aaaactgtga acgcaaatca gcatatgcag caaaataaag tggtttgtga
     7141 attcgataac catatttcgt gaagatttcc caacggtctg catttttgcg taatttatat
     7201 tggtcgcctt tagtaaactg caagccgtat tctgcggcag cccacatttg caataagtga
     7261 ttgttttttt tataatcagc attaaaacgc atgttaaata ataaagaaaa tgagttggtt
     7321 ccacctgctg cccagttttt aaagccagct tggttgaagg taacgccacc aataccgccg
     7381 aatttccaac cattcgttgt gtcttctaat tttttctttt catcggcaga taatgcttcg
     7441 cctttaattt gtttttctac ttgtgctaat gtagtcgtat gcatagctat tagagccagc
     7501 aacataaaaa gtaatttctt cataaataat atattttcct aatgagtgta gaagtaaatt
     7561 tcaagcaatt cgttgcaaga aatttactta tttattctag caacaaagat aagtagttgt
     7621 aattgaagat aatattatta ataaagtatg caagtttatt ttttgaacag caattgtgtt
     7681 agtttttttc ttttaaataa ttgaaaattt tacgatacaa gtcataatat agcttgctgc
     7741 tgccgaggcc gtgtgcctca tctggatata gataaagctg atagtttttg ttcgaagtat
     7801 ttagcgattg tataagacgt aaggtgtgtt gtaattgtac attgtcatca gcagtgccat
     7861 gtattagcat taaattacct tgcaagtttt ttgcaagttt ttctggttga aaattagcgt
     7921 aagcactagc attttcagta ggtgtgcgca tatagcgttc tgtataaatc gtgttataca
     7981 acttccaatt tgtaatagga gctgctgcga ttgctgtttt aaatatggaa ttgggttgca
     8041 ataggcagtt tgctgccaaa tagccaccat aactccagcc ataaatacaa acattatttt
     8101 tgtcaattgg aaattgatta cataaatatt gcactgcttg tgtttggtct tccgtttcta
     8161 attttcccaa ttgtttaaat gtcgcttgct taaatttttt gcctctgccg tcagtacctc
     8221 tattgtcaat acatgcaaca aaatagcctt gttctgccag aaaatttaaa aataaatctc
     8281 ttgtgccact ccatttattt tcgacagatt gactttctgg tccgccgtaa gtgtatataa
     8341 ataaaggata tttctgagtg gtatcgattg cttttggagt gatgatccac gcatttagat
     8401 aattatcatt attcggaatt tgaatgaact ttttttgtgg aatattttct gttttcttct
     8461 gaatgtcttg attgtttact aagacaatag ttgccgtgtc gtttgtattt cgtatgctat
     8521 atgttggcgg cgtagttgct gatgaaaact ggtgtaagta gcgtgaaaaa tctttagcaa
     8581 aaatagcact attgctgcca ttctgattgg atagtttttc tttgtcgtgt gtgaatagat
     8641 gaatacgata aatagcagtt tctatctcat ttccttcatt gctttgataa tatatccaat
     8701 gctttgtttc atcaatgcca tatagttgtt ttatctcgta atttccattt gtaatttgtt
     8761 gcaatagctt tccattttca tcataatgat agagatgatt gtatccattt ttagcggaag
     8821 taataataaa ggaattgttg tttaaaaatg tgagcgcagt aggaatattt acataggcag
     8881 tgtccgtttc aaggtatagc tgtcttgtct tttttgagct tgtgttatag gctactaagc
     8941 gcatcgtatc ttggtttcta ttcattaaga gcattattaa ttcagtgccg cttttattcc
     9001 aattaatgcg tggaatatat tcgtatgcat agggaatcgc aaccgttttt gttttcttat
     9061 tttttaactg gtaaatatgt acactcagct tggcattttt ctcgccaact tttggatatt
     9121 t
//
LOCUS       DAOQQV010000107         8942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_2976_length_8942_cov_6.822437, whole genome shotgun sequence.
ACCESSION   DAOQQV010000107 DAOQQV010000000
VERSION     DAOQQV010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2976_length_8942_cov_6.822437"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLL59_10060"
     CDS             complement(<1..740)
                     /locus_tag="PLL59_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HMY24194.1"
                     /translation="MPFKKDNKINTEFKTITLKLASPDSILERSYGEVKKPETINYRT
                     YKPEMEGLFCERIFGPTKDYECFCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWYFKSLPNKTGYMLGLSSKNVEAIVYYERFVVVQAGITEDGVPVNGESKK
                     TKYLDLLTEEEYLDILDALPKDNQLLADDDPNKFIAKMGGDAIHALLSRIDLKKLSGE
                     LRHKASHESSQQRKSEALKRLSVVEAFR"
     gene            complement(790..4623)
                     /gene="rpoB"
                     /locus_tag="PLL59_10065"
     CDS             complement(790..4623)
                     /gene="rpoB"
                     /locus_tag="PLL59_10065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HMY24195.1"
                     /translation="MSKKTEKSGQLTAGRINFSKISHPYDYPNLLDIQVKSFQEFLQL
                     ETTPDKRSKEGLYKVFSENFPITDARNIFLLEFLDYYVDPPRYTVDECIERGLTYSVP
                     LKAKLRLSCNDEEHIDFQTIVQDVFLGNIPYMTDKGTFIINGAERVVVSQLHRSPGVF
                     FGMSYHPNGTKIFSARVIPFKGAWMEFATDINNVMYAYIDRKKKFPVTTLLRAIGFDS
                     DKDILKLFGLSDEIDVNKKSLEKHIGRRLAARVLKSWMEDFVDEDTGEVVSIERNEVI
                     LERDTYLDEDNIQLILETGVKTIILQKENTDEDYSIIFNTLQKDTSNSEIEALKHIYR
                     QLRGSDPPDDDTARGIIEKLFFSDKRYDLGFVGRYKINKKLGLGIDAKNIVLTKEDIV
                     SIVKYLIKLVNQKAEIDDIDHLSNRRVRTVGEQLFAQFGVGLARMARTIRERMNVRDN
                     EVFTPIDLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRER
                     AGFEVRDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKVNEMGFIETPYRKVSKSKVE
                     GKVEYLTAEEEDERVIAQSNIQLDDKGTFADKKVKCRFQGDFPIYDADSEEVQTVQNM
                     DVAPNQIVGVSASLIPFLENDDANRALMGSNMQRQAVPLMRCESPIVGTGIEARVASD
                     SNVLINAERSGVVEYVDANEIRVRFDRTENEKLVSFEDDVKVYKLTKYARTNQATCIN
                     LRPIVRKGEKVAKGQILCEGYATQKGELALGKNLLVAFMPWKGFNFEDAIVINERVVR
                     DDIFTSIHIEEYEMDVRDTKLGEEELTNDIPNVSEDATRNLDENGIIRVGAKIKEGDI
                     LIGKITPKGETDPTPEEKLLRAIFGDKAGDVKDASYKAPPSTEGIVVTTKLFQKAKKD
                     KNSKVREKAELEKAEKVHVQNLEEIKKVLVAKLMQLVGGKTTIGIKDIFGEEAVPKGT
                     KFSEKLLGNLNYQNLDTAEWTKEEETNELIKRLIHNFNIKFNEEKGRFLRDKYAITVG
                     DELPSGVLKLAKVYIAQKRKLRVGDKMAGRHGNKGIVAKIVRDEDMPFLEDGTPVDVV
                     LNPLGVPSRMNIGQIYETVLGWAGKELGVKFSTPIFDGANVEEIEHYIEEAGLPRLGS
                     TYLYDGETGQRFDQKTTVGVIYMIKLSHMVDDKMHARSIGPYSLITQQPLGGKAQFGG
                     QRLGEMEVWALEAYGASNILQELLTVKSDDIQGRAKAYEAIVKGDNLPVPGIPESFNV
                     LVHELRGLALDLDFE"
     gene            complement(4714..5091)
                     /gene="rplL"
                     /locus_tag="PLL59_10070"
     CDS             complement(4714..5091)
                     /gene="rplL"
                     /locus_tag="PLL59_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HMY24196.1"
                     /translation="MADIKAFAEQLVNLTVKEVNELAEILKSEYGIEPAAAAVAVAAA
                     PGGGGGAAVEEKTSFDVILKDGGAQKLGVVKVVKEITGLGLKEAKDLVDGAPKPVKEG
                     VSKDEAESIKAKLEEAGASIELK"
     gene            complement(5118..5639)
                     /gene="rplJ"
                     /locus_tag="PLL59_10075"
     CDS             complement(5118..5639)
                     /gene="rplJ"
                     /locus_tag="PLL59_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HMY24197.1"
                     /translation="MTKTEKQATIDTLSKKFEEANFFYFTDTSGLTVEKVNQLRKICF
                     EKGINLQVAKNTLIKKALVAGGKFSDELEPVLHGPTAVMFTETGNVPAKVIKQFRASG
                     NEKPALKAAYIDSAVFVGENQLEVLVNVKSKEELVGDIVALLQSPAKNVISALKSSGG
                     KLAGIVKTLQEKN"
     gene            complement(5664..6359)
                     /gene="rplA"
                     /locus_tag="PLL59_10080"
     CDS             complement(5664..6359)
                     /gene="rplA"
                     /locus_tag="PLL59_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HMY24198.1"
                     /translation="MKVVKKRKAINAKVEKGKVYSLEQATALVKDVNTTKFDASVDVH
                     IRLGVDPRKPDQALRGTVSLPHGTGKTKTVLVLCPADKEAAAKEAGADYVGLDEYLEK
                     IGGGWTDIDVIIATPSVMPKLGRLGKILGPRNLMPNPKSGTVTENVADAVREVKKGKI
                     SFKIDKFGIIHTSVGRVSFNPSQLFDNAKEMVSTLAKMKPSSAKGIYFKSIFMATTMS
                     PSIEVDVKSIPGI"
     gene            complement(6425..6868)
                     /gene="rplK"
                     /locus_tag="PLL59_10085"
     CDS             complement(6425..6868)
                     /gene="rplK"
                     /locus_tag="PLL59_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HMY24199.1"
                     /translation="MAKEIETFIKLQVKGGQANPAPPIGPALGSKGVNIMEFCKQFNA
                     ATQDKQGKILPCVITVYKDKSFTFVIKTPPASALLLEYSKVKQGSKIPNRNKVGSVTW
                     DQVKEIAEIKMPDLNCFTVTSAMKMVAGTARSMGITVTGEAPFQA"
     gene            complement(6871..7416)
                     /gene="nusG"
                     /locus_tag="PLL59_10090"
     CDS             complement(6871..7416)
                     /gene="nusG"
                     /locus_tag="PLL59_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HMY24200.1"
                     /translation="MSDEKKWYVLRVISGKEKKLREIIEKEIKLSNWTDIIPQVIVPT
                     EKVYKLKGGKKVIHEKNFFPGYLMLEADPKRFNGDIVLHIQNMTNVISFIERNKPIPL
                     KKAEVNRILGKVDEMEEVGGTVNEPFLVGEDVKITDGPFNDFTGTIEEIMEDKKKLRV
                     VVKIFGRKTPVELNFMQVEKQ"
     gene            complement(7437..7634)
                     /gene="secE"
                     /locus_tag="PLL59_10095"
     CDS             complement(7437..7634)
                     /gene="secE"
                     /locus_tag="PLL59_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HMY24201.1"
                     /translation="MDNVKTYLIASYEELTSKVTWPTWKELQANAVIVAIASFIIAFI
                     IGLMDMLSNMLFSDIIYKIAG"
     gene            complement(7651..7721)
                     /locus_tag="PLL59_10100"
     tRNA            complement(7651..7721)
                     /locus_tag="PLL59_10100"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7687..7689),aa:Trp,seq:cca)
     gene            complement(7780..>8942)
                     /gene="tuf"
                     /locus_tag="PLL59_10105"
     CDS             complement(7780..>8942)
                     /gene="tuf"
                     /locus_tag="PLL59_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HMY24202.1"
                     /translation="KPHVNIGTIGHVDHGKTTLTAAITYVLSKSGLAEKKDYDSIDAA
                     PEEKERGITINTAHVEYQTQSRHYAHVDCPGHADYIKNMITGAAQMDGAILVVASTDG
                     PMPQTKEHILLAAQVGVPRMVVFMNKVDLVDDPELLELVEMDIREELTKRGYDGDNTP
                     IIQGSAIKALQDDAEGTAQIEALMKAVDEWVPLPPRPIDQPFLLPVEDVFSITGRGTV
                     ATGRIERGVVKTGEPVEIVGLQEKSMTSTCTGVEMFRKILDIGEAGENVGILLRGIEK
                     TDIKRGMVICKPGSVTPHTEFKCEVYILSKDEGGRHTPFFNKYRPQFYLRTTDVTGEI
                     ELPAGVEMVMPGDNVTLTVKLIYPVALEKGLKFAIREGGRTVGAGQVTEIIK"
BASE COUNT         2485 a         1839 c         1466 g         3152 t
ORIGIN      
        1 tcacggaatg cttctacgac gctcagacgt tttaacgctt ctgatttacg ttgttgagat
       61 gattcatgcg atgctttatg gcgcaattca cctgataatt ttttcaaatc gattcttgac
      121 aataaagcat ggattgcatc gccacccatt ttagctataa atttatttgg atcatcgtca
      181 gctaataatt gattgtcttt tggcaatgca tctaaaatat ctaaatactc ttcttctgtt
      241 aagaggtcta agtattttgt ttttttgctt tcgccattta ctggcacgcc atcttctgta
      301 ataccagctt gtacgactac aaaacgttcg tagtaaacta tggcttctac atttttagaa
      361 gacaaaccca acatgtaacc ggttttattc ggcaaagatt tgaagtacca aatatgtaca
      421 acaggcacca ctaacttgat gtgacccatt ctttcgcgac gtactttttt ctctgttact
      481 tccacaccgc agcggtcgca tacgatgcct ttgtaacgga tacgtttgta cttaccgcaa
      541 aaacattcat agtcttttgt tggaccgaaa atacgctcgc agaacaaacc ttccatttca
      601 ggtttgtagg ttctgtagtt gatggtttcc ggttttttta cttcgccgta tgaacgctct
      661 aatatagaat ccggagaagc gagtttcaac gtaatcgtct taaactctgt gttgattttg
      721 ttatcctttt taaaaggcat actagttatt tttaaatgct taaaaatttt atttcgaaaa
      781 aacttacgac tattcaaagt ctaaatccaa cgccaaacct cttaactcgt gtaccaatac
      841 attgaatgac tcaggaatac caggtacagg taagttatcg cctttaacga tcgcttcgta
      901 agcttttgct cttccttgaa tatcatctga tttcacagtt aataactctt gtaagatatt
      961 agatgcaccg tatgcttcga gtgcccaaac ttccatctca cctaaacgct gaccaccaaa
     1021 ttgtgcttta ccaccaagcg gttgctgcgt gattaatgag taaggtccga ttgaacgagc
     1081 atgcatttta tcatctacca tgtgtgataa cttaatcata tagataacac ctactgttgt
     1141 tttttggtcg aaacgttgac cggtttcgcc atcatataag taggtactac caagacgtgg
     1201 caatcctgct tcttcgatat agtgttcaat ttcctcaaca ttagcaccgt caaagattgg
     1261 tgttgagaat ttaacgccta attctttacc tgcccagcct aatacagttt cataaatctg
     1321 accgatgttc atacgagaag gcacacctag tggatttaat acaacgtcaa caggcgttcc
     1381 atcttctaaa aatggcatgt cttcatcgcg aacgatttta gcaacgatac ctttatttcc
     1441 gtggcgacca gccattttat cacccactct caacttacgt ttttgagcga tgtaaacttt
     1501 agccaatttt aatacaccag atggtagctc atcacctaca gtgatagcat atttatcacg
     1561 taagaaacga cctttttctt cattgaactt aatgttgaag ttatgaatta agcgtttaat
     1621 caattcattt gtttcttctt ctttcgtcca ctcagcggtg tctaagtttt ggtagtttaa
     1681 gttgcctaat aatttttcag agaatttagt gccttttggt actgcttctt caccaaaaat
     1741 atctttaata ccaatagtag tttttccgcc tactaattgc attaatttcg ctaccaatac
     1801 ttttttaatt tcttctaagt tctgtacgtg tactttctct gctttttcca attctgcttt
     1861 ttctctaact ttagagtttt tatctttttt agctttttgg aatagcttag ttgtaaccac
     1921 aataccttct gttgatggtg gtgctttgta agaagcatct tttacatcac cagctttatc
     1981 gccgaagata gcacgcaata atttttcttc aggtgttgga tcagtttccc cttttggagt
     2041 aatttttcct attaaaatat caccttcttt gatttttgca cctacgcgaa tgataccatt
     2101 ttcatctaag ttgcgtgttg catcttcgct tacatttgga atatcattcg ttaattcttc
     2161 ttcgcctaat tttgtatcac gcacgtccat ttcatattct tcaatatgaa tagaagtgaa
     2221 gatgtcatcg cgcactactc tttcgttgat aacgatagca tcttcgaagt tgaacccttt
     2281 ccaaggcatg aaggctacta ataaattttt acctaatgct aattcacctt tttgtgtggc
     2341 ataaccttcg cacaaaatct gaccttttgc tactttctca cctttgcgta caattggtcg
     2401 caagttgata caagtggctt ggttagtacg tgcatatttt gttagtttat acactttcac
     2461 atcgtcttcg aagcttacta atttttcatt ttcagttcta tcaaaacgaa cgcgaatttc
     2521 attggcatca acatattcta ctactcctga acgttcagcg ttaattaaca catttgagtc
     2581 agatgccaca cgagcttcaa tacctgtacc aacgattggc gactcacaac gcattaacgg
     2641 cactgcctga cgttgcatgt ttgagcccat caatgcacgg ttagcatcgt cattttctaa
     2701 gaaaggaatt aaagaagcag agacaccaac gatttggtta ggcgctacgt ccatgttttg
     2761 tacagtttgt acttcttcgc tatctgcatc ataaattggg aaatcacctt ggaaacggca
     2821 tttcactttt ttgtcagcaa atgttccttt atcgtctaat tgtatatttg actgtgcaat
     2881 tactctttca tcttcttctt cagccgttaa atattcaact tttccttcta ctttgctttt
     2941 tgacactttg cgataaggtg tttcgataaa gcccatttca ttcacttttg catgtacgca
     3001 aagtgtagaa atcaaaccga tatttggacc ttccggcgtt tcaattgtac acaaacgtcc
     3061 atagtgtgag tagtgaacgt cacgcacctc gaaacctgct ctttcacgag ataaaccacc
     3121 tggtccgagt gcagaaatac gacgtttgtg tgtaatttcc gacaatggat tggtttggtc
     3181 taagaattga gataattgtg atgtaccaaa gaaagaattg ataacagaag aaagcgttct
     3241 tgcgttaatt aaatctattg gtgtgaacac ctcattatca cgaacattca tacgctcacg
     3301 aatagtacgc gccatacgtg ctaagcctac gccaaactga gcaaacaatt gctcgcctac
     3361 tgtgcgcacg cgtctgttgc tcaagtggtc gatatcatcg atttctgctt tttggttgac
     3421 taattttatc aagtatttaa cgatagaaac gatatcttct tttgttaata cgatattttt
     3481 agcatcgatg cctaagccca attttttatt gattttgtaa cgacctacaa aacctaaatc
     3541 gtagcgttta tcggagaaga ataatttctc gataatacca cgagctgtat catcatctgg
     3601 tggatcagaa ccacgtaatt gacgatagat atgttttaac gcttcaattt ctgaatttga
     3661 cgtatctttt tgtaaagtat taaagatgat agaataatct tcatctgtat tttctttttg
     3721 taagatgatt gtttttacgc cagtttctaa aataagctgg atattatctt catctaaata
     3781 cgtatcgcgt tctaagatga cttcattacg ttcgatagaa actacctcac cagtatcttc
     3841 atctacgaaa tcttccatcc atgatttaag cacacgtgcc gccaagcgac gaccgatatg
     3901 tttttctaaa gattttttgt ttacatcgat ttcatctgac aagccaaaca atttaagaat
     3961 gtctttatct gaatcgaatc ctatagcgcg taacaaagtt gttacaggga attttttctt
     4021 acggtcgatg tatgcataca ttacattgtt aatgtctgta gcaaattcca tccaagcacc
     4081 tttaaacgga ataactcttg cagagaatat ttttgtaccg tttggatggt agctcatacc
     4141 aaaaaacaca cctggagagc ggtgtaattg agaaacgaca acacgttcgg caccattaat
     4201 tataaaagta cctttgtctg tcatataagg aatatttcct aagaacacat cttgaacgat
     4261 ggtttggaaa tcgatatgct cttcatcatt acatgataag cgtaattttg cttttagcgg
     4321 aacgctatat gtcaaacctc tttcgataca ctcatcaacg gtgtagcgag gcgggtcaac
     4381 ataataatcc aagaactcta acaagaagat atttcttgca tctgtgattg gaaagttttc
     4441 ggaaaacact ttatacaaac cttcttttga gcgtttgtca ggtgtggttt ccaattgcag
     4501 gaattcctga aatgatttta cttggatatc caacaaattt ggatagtcgt acggatgact
     4561 aattttactg aaattgattc ttcctgcagt taattgacct gatttttctg tttttttaga
     4621 catgcaatct tattaaaatt tataaaaaca gtaaaaagtg tctcggcaac aaaaacagtt
     4681 gccggacact ttaaatattt gattttgaat gaattacttc aattcaatag atgcgccagc
     4741 ttcttctaat ttagctttga ttgactcagc ttcatcttta gaaacacctt ctttaactgg
     4801 ttttggtgcg ccatcaacta aatctttagc ttcttttaag ccaagacctg tgatttcttt
     4861 aaccacttta actacgccta atttttgtgc gccaccatct tttaagatta cgtcaaaaga
     4921 tgttttttct tctacagcag caccaccgcc accgcctgga gcagcagcaa ccgctacagc
     4981 agcagcagct ggttcgatac catattcaga ttttaagatt tctgcaagtt cgtttacttc
     5041 tttaactgtt aagtttacta attgctctgc gaatgcttta atatcagcca ttgtttaaaa
     5101 atttaaaatg ttataaatta atttttttct tgtaatgttt taactattcc cgctaatttg
     5161 ccgccacttg atttaagtgc tgagataacg tttttcgctg gagattgaag taatgcaacg
     5221 atgtcgccta ctaattcttc tttagattta acgttaacta atacttctaa ttggttttcg
     5281 ccaacaaata ctgcagaatc gatatacgca gcttttaaag ctggtttttc gttacctgat
     5341 gcacggaatt gttttattac tttagcaggt acattgcctg tttcggtaaa cataactgct
     5401 gtaggaccgt gtaatacagg ttctaattcg tcagaaaatt taccaccagc aactaatgct
     5461 tttttgatta atgtgttttt tgcaacttgt aagttgatac ctttttcgaa acaaattttt
     5521 cttaattggt ttactttttc tactgttaag ccagacgtat ctgtaaaata gaagaagttt
     5581 gcctcttcga attttttcga caatgtatcg attgttgctt gtttttcggt cttagtcatt
     5641 tttttctttt ttagaatgaa ctattaaatt cccggaatcg attttacgtc tacttcgata
     5701 gaaggactca tggttgtagc cataaagatg cttttaaaat agataccttt agcagaagat
     5761 ggtttcattt ttgctaatgt gcttaccatt tctttagcat tatcgaacaa ttgagatgga
     5821 ttaaaagata cacgacctac agaagtgtgg atgataccga atttatcgat tttgaaagaa
     5881 attttacctt tcttaacctc acgcacagca tctgcaacgt tttctgttac cgtaccagat
     5941 ttagggtttg gcattaagtt acgtggtcct aaaattttac ccaatctacc taatttaggc
     6001 atgacacttg gagtagcaat tatcacatcg atatctgtcc aaccgccacc gattttttct
     6061 aaatactcat ctaagccaac ataatcagca ccagcttctt tagctgcagc ttctttatcg
     6121 gcaggacaaa gtactaaaac agttttggtt ttaccagttc cgtgtggtaa gcttactgta
     6181 cctcttagag cttgatctgg tttacgtgga tcaacaccta gacgaatgtg tacgtcaaca
     6241 gaagcgtcaa atttagttgt gtttacgtct tttactaaag cagttgcttg ttctaaagaa
     6301 tacactttac ctttttcaac tttagcgtta atcgcttttc ttttttttac aactttcatt
     6361 tttacaaaaa tttgatgaga taatagcgtt ccaatgatga gtggaactct ctccgcctat
     6421 tttactaagc ttggaatggt gcttcacccg taaccgtgat tcccatactt cttgcagtac
     6481 ctgctaccat tttcattgct gacgtaactg taaagcaatt taaatctggc attttgattt
     6541 ctgcaatttc tttcacttga tcccaagtta cagagcctac tttatttctg ttaggaattt
     6601 ttgagccttg tttaacttta gaatactcca acaataatgc ggaagctggt ggagttttga
     6661 tcacgaatgt gaacgattta tctttataca ccgtaattac acacggtaat atttttcctt
     6721 gtttatcttg agttgcagcg ttaaactgtt tgcaaaactc catgatgtta acacctttag
     6781 aacctaatgc aggtccgatt ggtggtgctg gatttgcttg tccgcctttt acttgtaact
     6841 taataaacgt ttcgatttcc tttgccataa ctattgtttt tcaacttgca taaaatttaa
     6901 ctccacagga gtttttcttc caaatatttt tacaactact ctcagtttct ttttgtcttc
     6961 cattatctct tcgatagtac ctgtaaagtc attgaatggt ccgtctgtaa ttttcacatc
     7021 ctcaccaact aagaatggtt cattgacagt accgcctact tcttccattt cgtcaacctt
     7081 gcctaatatt ctatttactt cggctttctt caatggaatt ggtttatttc tttcaataaa
     7141 gctgattacg tttgtcatat tttgaatatg taaaacgata tcgccattga atcttttagg
     7201 atctgcttcc aacattaaat aacctggaaa aaagtttttc tcatgaatta cttttttacc
     7261 accttttaat ttatatactt tttctgtagg cacaattacc tgcggaataa tatcagtcca
     7321 gttagaaagt ttaatttcct tctcgataat ctctcgaagt tttttttcct ttccactgat
     7381 tactctcagt acgtaccatt ttttttcgtc agacatctgt atatttctaa agaaggttaa
     7441 ccagctattt tataaataat atcactaaac aacatattcg acaacatatc cattagccca
     7501 atgataaatg caataataaa tgaagcaatc gcaacgatta ctgcattggc ttgcaattct
     7561 ttccatgttg gccaagtcac ctttgatgtt aactcttcgt aagaagctat caaatatgtt
     7621 ttaacattat ccatctttac ttttcttttg cacgggcgga gagattcgaa ctcccatcag
     7681 cggttttgga gaccgacatt ctacccttga actacgcccg tctaaaaagt cattagccac
     7741 tcatcgtttt aaatgactaa tgactaatga cggtttaatt tatttaatta tttcagttac
     7801 ctgaccagca cctactgttc taccaccttc acggatagcg aacttcaaac ctttctctaa
     7861 tgcaactgga tagattaatt taactgttaa agttacatta tcaccaggca ttaccatttc
     7921 tacgcctgct ggcaattcaa tctcacctgt aacgtctgtt gttcttaaat agaattgagg
     7981 acgatattta ttgaagaatg gtgtatgacg accaccttca tctttagata agatatacac
     8041 ttcacatttg aattctgtgt gaggtgttac agaacctggt ttgcagatta ccataccacg
     8101 tttgatatct gttttttcga taccacgtaa taaaatacct acgttttcgc cagcttcacc
     8161 aatgtctaat attttacgga acatttccac acctgtacaa gtagatgtca ttgatttttc
     8221 ttgcaaacct acgatttcta ctggctcacc agttttaact acacctcttt cgatacgacc
     8281 tgtagcaaca gtaccacgac cagtaataga gaatacgtct tcaacaggca ataagaaagg
     8341 ttggtcgatt ggacgtggag gtaaaggaac ccactcatca actgctttca ttaatgcttc
     8401 gatttgagct gtgccttcag catcatcttg taatgctttg atagcagaac cttgaatgat
     8461 tggtgtgtta tcaccatcat aaccacgttt tgttaattct tcgcggatat ccatttctac
     8521 tagctctaat aattctggat cgtcaaccaa atccacttta ttcataaaaa ctaccatacg
     8581 aggcacacct acttgtgcag ccaataagat atgttcttta gtttgtggca ttggtccatc
     8641 tgtagaagct accactaaga tagcaccgtc catctgagcc gcacctgtaa tcatattttt
     8701 gatataatcc gcgtgaccag gacagtcaac gtgtgcatag tgacgagatt gtgtttggta
     8761 ctctacgtga gcagtattaa tagtaatacc tctttctttt tcctctggag cagcgtcgat
     8821 tgaatcataa tcttttttct ctgctaaacc agattttgac aacacatacg tgattgcagc
     8881 tgtcaaagta gttttaccgt ggtcaacgtg accgattgtt ccgatgttaa cgtgcggctt
     8941 at
//
LOCUS       DAOQQV010000108         8818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3049_length_8818_cov_7.353418, whole genome shotgun sequence.
ACCESSION   DAOQQV010000108 DAOQQV010000000
VERSION     DAOQQV010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3049_length_8818_cov_7.353418"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLL59_10110"
     CDS             complement(<1..529)
                     /locus_tag="PLL59_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24203.1"
                     /translation="MQTNSIAQTYNDGRISLKVWVHKVWSNANCGEIGNQEYVIKNIQ
                     ARVLGSGGNYITSPSGNMITFDGDNNRYWNASGGGAFTASGFPFGGYDAAGFGYKLLD
                     VNYTSTEAPFAFDVIIGQAYEDDCDGTLLSCNQGSRALYDKCCCVNLPLIGTVCAQSD
                     EYQGTSSWTGVNFRGG"
     gene            complement(597..1160)
                     /locus_tag="PLL59_10115"
     CDS             complement(597..1160)
                     /locus_tag="PLL59_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24204.1"
                     /translation="MNKIVQVLLVYFLGILYSFACDDHNTNNVLNIIPQKTYTEKEAR
                     ELDFTQNFDTKLFIKNYTDAINNRNLSLLENYFIEESSYKQLLQQLMDDGKEMCLYAE
                     EKVDQNYFKNQIFNLILNNTTITSIINTNNISFEGCAQIPFIKMNFDVVYTRDGNENN
                     VRLVIILWKAYNGKFNVYAQVGNFFKI"
     gene            1717..1914
                     /gene="rpmI"
                     /locus_tag="PLL59_10120"
     CDS             1717..1914
                     /gene="rpmI"
                     /locus_tag="PLL59_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HMY24205.1"
                     /translation="MPKMKTNSSAKKRFKVTGSGKIQSGQAYKRHILTKKSTKQKRNL
                     TGSTIVRKCDEGNIKALLCMN"
     gene            2024..2368
                     /gene="rplT"
                     /locus_tag="PLL59_10125"
     CDS             2024..2368
                     /gene="rplT"
                     /locus_tag="PLL59_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HMY24206.1"
                     /translation="MPRSVNSVAAKDRKKKVYKLAKGYYGRRKNVWTVTKNAVEKGLT
                     YAFVGRKLKKRDYRSMWIVRINAAVRPFGLSYSVFMNKLANAGIEINRKALADLALND
                     PKAFEAIVNQVK"
     gene            complement(2497..4737)
                     /locus_tag="PLL59_10130"
     CDS             complement(2497..4737)
                     /locus_tag="PLL59_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY24207.1"
                     /translation="MKRIFFLILFSIGYFSNFAQVMPAAYQQGRDLYQRLAYSEAIPF
                     LIKECTGNKNKFLEADIMLADCYRQTNQYKLAEGEYAIVCQDVRLKDDKQKLYYAQVL
                     QTNQKYDKAAEWYGKYLEKFPMDSRAKNQKLASENVAQFTSGKKLYITNLPFNTNGYD
                     FGACINKNTLYFTSTGGKVDDVKAKDINAWTGERFMNLYSTEIQQSDTASDIFTTAAI
                     LPNAVNTKYNEGPLCFNKDNTKVYFTRNQYNPEEKAKIAYSKEREANLSIYEATIENG
                     NWTKIKELPFNSKQFSCGHPALDEDNKTLYFSSTMPGGFGGSDIWKVKYDGDKWEKPV
                     NIGTSINTEGEEMFPTFDNSKTFYFASDGQGGIGGLDIFSSKMTDNGIVDLKNLGTPI
                     NSSYDDFAYLINDDKTVGFFSSDRPNGKGEDDIYRFADIEYKLEILVIHKHNKSAIAN
                     AHVQVKNGEQIKNDDYTNTDGKLIIPVEPGTTYKLQADALGYLPATVSKTIIENEKQA
                     LQKVVIELEPMVMQVKVINDVTKAPIPNAKLTCSSPCQQSVKVNSTDENGKINYAVQD
                     KCTYTLEASAKSYLPKPASQYTITLVDTTYVIIELTQITEKAIALNNIYYDFDKWNIR
                     PEAEEDLNMLLAFMKNNPDAIVELSSHTDARGDDAYNLSLSQKRAQSAVTWLVMHGIS
                     DSKIKPVGYGETKPVNNCRNNIKCTEEEHQRNRRTEFKVLNAGEIINSTTKERIVVDP
                     CNNCLF"
     gene            complement(4787..5758)
                     /locus_tag="PLL59_10135"
     CDS             complement(4787..5758)
                     /locus_tag="PLL59_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HMY24208.1"
                     /translation="MKKYSIIWILLLLLTASTLKAQQEPYNTMFAFNKLPVNPGYTGG
                     KDVLSIRALYRHQWVNLPGNPQTINFNIHSPLKYDNVALGLSIINDRLGATNMTHLNT
                     TFAYRLPFKNDTKLSFGISAGMMIFNAKLTELDAVDVSDPLISQNLKGVRPNIGAGVY
                     YYGSKFYVGLSIPNMIPVGLFKKKDNFAGELRMQQLTSLLLMGGYTFEIGKKKNFWLQ
                     PQLMLKYMPNSKYELPLEVDANLTFTFFKIFGLGLTYRTGIADPYQNRESVDVMAIFY
                     LPKDITIGYAYDQTISKIKTFNKGTHEIMLGYDVSWKKKGIRTPRYF"
     gene            complement(5779..>8818)
                     /locus_tag="PLL59_10140"
     CDS             complement(5779..>8818)
                     /locus_tag="PLL59_10140"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY24209.1"
                     /translation="RTWTATDECGNSSTSQQTIRVNDSISPVFIEIPEDITLACASEV
                     PAASIENVSATDNCSGEVQITVEDFIKPDSCANKYVLKRVWKATDICGNTSTASQNII
                     VYDSISPIITGVPEDIILSCANDVPVASIGDVDVADNCTSEISISVNDEVSNQTCINK
                     FIITRTWTATDECGNTSSAVQFIHVDDTIAPTFIGNLPSNLNVCSEVPNAPEITAVDN
                     CSDVDVDYTETIDSTSVPGVKTYIRTWTATDQCGNTSTHQQTIVVSPTIHITLNREIC
                     EGESVSIGENVYGVSGSYQVAFVSVDGCDSIVTLHLIVHPNKQTEIDAVICQGEQIVV
                     GENVYTESVTNEIIHLQTSFGCDSMITLNLLVLNNETIKINRSICAGEFVEIEGVKYY
                     EAGVYNIPIHNQQCEGIVQLNLTVIQATSATLNRTICEGDVVTIAGHIFSTNGVHHIT
                     IPNAAGCDSLITLNLTVNPKSEVEIERVICSGQSIIIGDQQFNTSGNYTVHLLNSNAC
                     DSLIKLHLIVIDNNDTIKINRTICDGEEVIFNEQVFNETGVYYIPISNERCGAIVEFN
                     LHVNDVSATSLQETICEGEAVVVGNQTFTSSGTYKVILQNSNGCDSVVILDLTVSTGG
                     TKTQIDTTVCFGETVKIAGQTFTSSGTYQVIYPVEVCRDTLLVNITVNPVSQSRLDTS
                     IFDCLGGINIGGEVYIEPGNYQIHILNSLGCDSLVNLNLRVRICDTIPIGQDTTIYDT
                     LPVLTTHEICELSLPPSENVIISSCNDTSHTTLHGTWSINEQNCLVYTAGTLVGNDTL
                     CISACDPQTRQCNTTTVIITITGLPPIAADDCFEQEEENETNEQNKDVIIDVLDNDTD
                     PDQDALFVQSILVQPKYGIASIGNEGGNIIYTPNRNFCGRDTLYYVVCDNDDGCDTAM
                     VCLHIDCECIFPQVITPNNDGLNDNLFFPCLGNVEGAKLLVWHRWGLIVFEDDNYKND
                     WNGTLKGELLPAGTYWYSIQYIDPETKQEVKEVNYFMIIN"
BASE COUNT         2741 a         1576 c         1404 g         3097 t
ORIGIN      
        1 ctccacctct aaaatttacg cccgtccagg aactggtacc ttgatattca tcagattgag
       61 cacatacagt gccaatcaaa ggaagattta cgcaacaaca tttatcgtaa agggctctgc
      121 taccttgatt acaagacagt aatgtaccat cacaatcgtc ttcataggcc tgtcctatga
      181 ttacatcgaa tgcgaaaggt gcttccgtag aagtataatt aacatctaac aatttgtatc
      241 caaaaccagc ggcatcgtat ccaccaaaag ggaaaccact agcagtaaat gcaccgccgc
      301 cagaggcatt ccaatatcgg ttattatcac catcaaaggt gatcatattt ccagatggtg
      361 aagtaatgta attgcctcca cttcctaaga ctctggcttg aatatttttt attacatatt
      421 cttgattgcc gatttcgccg cagttagcgt ttgaccatac tttatgcacc catactttta
      481 gcgaaattct tccgtcattg tatgtttgtg ctatactgtt agtttgcata attatgcaag
      541 ttgtcaacag cagaatcaat ttctgagttt ggtatataat tttcatagtt atggttttat
      601 attttaaaga aattacctac ttgggcatat acattaaatt taccgttgta agctttccac
      661 aagatgatta caagtctgac attattttca tttccatcac gtgtatagac aacatcgaaa
      721 ttcattttta tgaatggaat ttgtgcacaa ccttcaaatg aaatattgtt cgtattaatg
      781 atggaagtaa ttgttgtatt attaagaatt agattaaaga tttgattttt gaaataattc
      841 tgatcaactt tttcttctgc gtataagcac atttcctttc catcatccat taattgttgt
      901 aataattgct tataagatga ttcttctata aagtaatttt ccaacagact taggttgcga
      961 ttatttatcg catctgtgta atttttaatg aataatttag tgtcgaaatt ttgggtgaaa
     1021 tcgagttctc ttgcctcttt ttctgtgtaa gttttttgag gtataatgtt gagtacatta
     1081 ttggtattgt gatcatcaca tgcaaacgaa tacaatatac ccaaaaaata cacaagtaat
     1141 acttgtacaa ttttgttcat aatttatggt tttgtccttt aaatatattt ataataaatg
     1201 aaaaagtcaa tagcaaatta aaaaaaaaca catatcatta tcatttgata aaaaaccaag
     1261 gaatcagcta tgctgaatct tattaaaatc gagtaaaaaa ttatatgaat cgattaagtt
     1321 gatataattg atattcatct tagcaattgt acggtatgat ttagatgtgt aatttatatg
     1381 ctattgagtg tcaaagacaa tttaattttt tagacaaatg atattagatt aaaaggacaa
     1441 gaacaagatt tcatgctgtc accttgtggc gagacgaaca agcgcttctt tcatacaatt
     1501 ttttacaact tttaatacaa tttctgtatc actttaaatt ttatttaagc tgcataaaga
     1561 attagcataa caattaactt tagtagtcat caatttttca gacaaataaa tttaacataa
     1621 aacttacatt ttcaattttt tgaattagat aattcactaa ttaattcgta tctttgcacc
     1681 ctattaaaag ttttataacc actaaattgc atttttatgc ctaaaatgaa aacaaactcg
     1741 agcgcgaaaa aacgtttcaa agtaacagga agcggtaaaa tccaaagcgg tcaagcatac
     1801 aaacgtcata ttttaacaaa gaaatcgacg aaacaaaagc gcaaccttac aggctctact
     1861 attgttagaa aatgtgatga aggcaatatc aaggcattgt tgtgcatgaa ctaagaaact
     1921 aatctgttgc ttagtaaata tttattagtc agcagtgaaa ataaacagac ataaggcaag
     1981 gcatcgccga tggtcgtatt attaacaata aaaaataaaa gttatgccac gttcagtaaa
     2041 ttcagtagca gcaaaagacc gtaaaaagaa ggtctacaaa ctcgccaaag gttattatgg
     2101 tcgccgtaaa aacgtttgga cagtaacgaa aaatgccgta gagaaaggtt taacatacgc
     2161 tttcgttggt agaaaattaa agaaaagaga ttacagaagt atgtggattg tgcgtattaa
     2221 tgcagcagtt cgtccttttg gcttatctta ttctgtattc atgaacaaat tagcaaatgc
     2281 aggcatcgag attaatcgta aagcattagc tgatttagca ttaaacgacc caaaggcgtt
     2341 tgaagcaatt gtgaatcaag taaaatagat ttagcttaca ttagtataaa tcctggcatt
     2401 agtcaggatt tttttattta tacagtttga tgtatataca acatcaacgc catgcaaaat
     2461 gcatggcgtt gacgtttgag gaagtgtatg tgtaaatcaa aacaaacaat tattacacgg
     2521 atctacaaca atcctttctt ttgtagtgga attaatgatt tcaccggcat ttaatacttt
     2581 aaattccgtt cttctatttc tttggtgttc ttcttctgta catttgatat tatttctgca
     2641 gttgtttaca ggttttgttt caccataacc tacaggtttt atttttgaat cgctgatgcc
     2701 atgcatcacc aaccaagtta cggccgactg tgcacgtttt tgcgataatg ataaattata
     2761 tgcatcatcg ccacgtgcgt ctgtatgtga gcttagttca acgatggcat caggattgtt
     2821 tttcataaaa gccagtaaca tgtttaagtc ttcttctgct tctggtctga tattccattt
     2881 gtcaaagtca taataaatat tatttaaagc aattgctttt tctgttattt gtgtcaattc
     2941 aataatgaca tacgtggtat caaccaaagt aattgtgtat tggctggcag gcttaggtaa
     3001 ataagatttt gcagaagctt ctaacgtata tgtacattta tcttgaacag cataattgat
     3061 tttgccattt tcgtctgttg aattgacttt tactgattgc tgacatggac ttgagcaggt
     3121 aagtttggca tttggaatag gtgcttttgt cacatcatta atcactttta cttgcattac
     3181 cataggttct aattcaatga ctactttttg caaagcttgt ttttcatttt cgataattgt
     3241 tttagaaaca gtagcaggca aatagcctaa tgcatcagct tgtagcttat aagttgtacc
     3301 aggctctaca ggtattatca acttaccatc agtatttgtg taatcatcat ttttaatttg
     3361 ttccccattt tttacttgta catgagcatt cgcaattgcc gatttattgt gtttatgaat
     3421 aaccaaaatt tctaacttgt attcgatatc tgcgaatcta taaatatcat cttcgccttt
     3481 accgtttggt ctatcgctag aaaaaaaacc aactgtttta tcatcgttga tgagataagc
     3541 aaaatcatcg tagctcgaat tgattggtgt acctagattt tttaaatcaa caatgccatt
     3601 atctgtcatt tttgaagaaa aaatatcgag accaccgata ccgccttgcc cgtcagaagc
     3661 gaaataaaat gttttagaat tatcgaaagt cggaaacatt tcttctcctt cagtatttat
     3721 ggaagtacca atatttacgg gcttttccca tttatcgcca tcgtatttta ctttccaaat
     3781 atcggagccg ccaaaaccac caggcattgt tgacgaaaag tacagcgttt tattatcttc
     3841 atctaaagca ggatgaccac agctaaattg tttactatta aaaggcaatt cttttatttt
     3901 agtccaattt ccattttcga tagtagcttc atatatgctt agattagctt ctcgttcttt
     3961 gctatatgct atttttgctt tttcttctgg attatattgg tttctggtga aatatacttt
     4021 tgtattatct ttattaaaac acagcgggcc ttcgttatac tttgtattca cagcatttgg
     4081 cagtatcgct gctgtagtaa aaatatcaga tgccgtatcg ctttgttgta tttctgttga
     4141 ataaagattc ataaaacgct cgcctgtcca agcattgata tcttttgctt ttacatcatc
     4201 taccttaccg ccagtagaag taaaatacaa tgtattttta ttgatgcaag caccaaaatc
     4261 atagccattg gtattgaaag gcaagttggt gatatataat tttttcccag atgtaaattg
     4321 tgctacattt tcgcttgcta gcttttgatt ctttgctctg ctgtccatcg gaaatttctc
     4381 taagtacttt ccataccatt ccgctgcttt gtcatatttt tgattcgttt gtaatacttg
     4441 agcataatac aatttttgct tatcgtcttt taatcgtaca tcttgacaaa cgattgcata
     4501 ttctccttct gctaatttat attgattggt ttgtctgtag caatctgcaa gcattatatc
     4561 agcttccaaa aatttatttt tatttccggt acattctttt atcaagaacg gaatagcctc
     4621 actatacgct aaacgttggt ataaatcacg gccttgttgg taggcagcag gcataacttg
     4681 cgcaaagttg gaaaagtagc cgatactaaa tagtatcaga aaaaatattc ttttcatgcg
     4741 tctaattttg tgatgagtaa tgcagtacat aagcttgatt tattgattag aaataacgtg
     4801 gtgttctgat acctttcttt ttccagctaa catcatatcc taacataatt tcgtgtgttc
     4861 ctttattgaa tgtttttatt tttgaaatgg tttgatcata agcatagcca atagtaatgt
     4921 cttttggtaa atagaaaata gccattacat ctacgctttc tctattttga taggggtcag
     4981 caattccggt tctgtaggtt aaacctaatc caaatatttt aaagaaagta aatgtaagat
     5041 ttgcatctac ttccaatggc aattcatatt ttgagttagg catatatttc agcattaact
     5101 gtggttgtaa ccagaaattc tttttctttc caatttcaaa agtatagcca cccattagca
     5161 ataaactagt gagttgttgc atacgaagtt cgccggcaaa gttgtctttc tttttaaaca
     5221 agcctacagg aatcatattt ggtattgaca atcctacata gaatttagag ccatagtaat
     5281 aaacaccagc gccaatattt gggcgaacac ctttcaaatt ctgtgaaata agtggatcgc
     5341 taacatctac cgcatcgagc tcagttaatt tagcattgaa aatcatcatg cctgcgctga
     5401 taccaaagct taatttagta tcatttttaa aaggcagacg gtaggcgaat gtggtattca
     5461 agtgtgtcat attggtagca cctaagcgat cgttgataat agacaagccc aatgcgacat
     5521 tatcatattt caatgggctg tgaatattga aattaatcgt ttgtggatta ccaggtaaat
     5581 tcacccattg atgtctatat aaggcacgaa tacttaatac atctttgcca cctgtatagc
     5641 caggattgac tggtagttta ttaaaggcaa acatcgtgtt atacggttct tgctgtgctt
     5701 ttaaggtact tgctgtaagc aataaaagca atatccagat tatactatat tttttcatat
     5761 cgtagaactt attagcgatt aattaattat cataaagtag tttacttctt ttacttcttg
     5821 tttggtttca ggatctatat attgaatgct gtaccagtaa gtgcccgcag gaagcaactc
     5881 tcctttcaaa gtgccattcc agtcgttttt atagttatca tcttcgaaca caatcaatcc
     5941 ccaacgatgc catactaata attttgcacc ttctacattg cctaaacaag ggaagaataa
     6001 attgtcattt aatccatcat tatttggtgt gataacttga gggaaaatac attcgcagtc
     6061 gatgtgtaaa cacaccattg ctgtatcaca tccgtcatca ttatcgcaaa caacataata
     6121 caaagtatct ctaccgcaga agtttctgtt tggtgtgtaa atgatgttgc caccttcatt
     6181 tccaatgctt gcaataccat atttaggttg aacaagaata gattgaacaa agagtgcatc
     6241 ttggtctgga tcagtatcgt tgtctaatac atctatgatc acatctttgt tttgctcgtt
     6301 agtctcattt tcttcttcct gttcgaagca atcatctgct gcaatcggag gtaatcctgt
     6361 tatcgtgatg atgaccgttg tggtgttgca ctgtctggtt tgaggatcac aagcacttat
     6421 acaaagcgta tcatttccga ccaatgttcc tgctgtatat acaagacaat tttgttcatt
     6481 gatagaccaa gtgccatgta atgttgtatg cgaagtatcg ttacaagaac tgataattac
     6541 attttcactt ggtggcagag acaactcaca aatttcgtgt gtcgttaaca ctggaagcgt
     6601 atcgtatatt gtagtatcct gaccaatagg aatggtatca caaattctta cgcgtaaatt
     6661 aagattaacc aagctatcgc aaccgagtga attaagaata tgtatttgat aatttcctgg
     6721 ttcgatataa acttctccac caatattaat accgcctaag caatcgaaaa ttgaggtatc
     6781 taatctagat tgagaaacag gattaacggt aatatttacc aataatgtat ctctacacac
     6841 ttcaactgga tagatgactt gatatgttcc tgaagacgtg aatgtttgac cagcaatttt
     6901 cactgtttct ccaaaacata ctgtcgtatc tatctgtgtt tttgtgccac ctgttgacac
     6961 tgtcaaatct aagattacaa cactatcgca tccattagaa ttttgaagaa taactttata
     7021 tgtacctgag ctagtgaaag tttgattgcc cacaacgaca gcttcacctt cacaaattgt
     7081 ttcttgtaat gacgttgcag aaacatcatt aacatgtaag ttaaattcaa caattgcacc
     7141 acatctttcg tttgaaattg gaatataata tactcctgtt tcattaaata cttgttcgtt
     7201 gaatattact tcttcaccat cgcaaatcgt tctattaatt ttgattgtat cattattatc
     7261 tattactatc aaatgtaatt ttattaaact atcacatgca tttgaattca acaagtgtac
     7321 agtataatta ccacttgtat taaattgctg gtcaccaata attattgatt gtcctgaaca
     7381 gatgacgcgt tctatttcta cttctgattt tggatttaca gtaagattaa gtgtaatcaa
     7441 actatcgcaa cctgccgcat ttggtattgt aatatggtgt acgccatttg tactaaatat
     7501 atgccctgca attgtaacta catcaccttc acaaatagta cgattcaagg ttgcagatgt
     7561 tgcttgtatt accgttaagt tcaactgaac tattccttcg cattgttgat tgtgaatagg
     7621 aatattatat acaccagctt cataatactt cactccttct atttcaacaa attcgccagc
     7681 acaaattgat ctattaattt taatagtttc attattcaat actaataaat tcaaagtaat
     7741 catactatca caaccaaatg atgtttgcaa atgaattatt tcatttgtaa cactttctgt
     7801 ataaacattt tcacctacaa caatttgctc accttggcat atcactgcat caatttctgt
     7861 ttgtttattt ggatgaacta ttaaatgtaa tgttacaata ctatcacatc catctacact
     7921 tacaaaagca acttggtatg aaccagaaac accataaaca ttttctccaa ttgacactga
     7981 ttctccttca caaatttctc tgttcaaagt aatatgtatt gttggactta caactatcgt
     8041 ttgttgatgt gtgcttgtat tgccacattg atctgttgct gtccacgttc taatataggt
     8101 ttttacacca ggtacagatg ttgagtcaat tgtttccgta taatctacat cgacatcact
     8161 acaattatca actgctgtaa tttctggtgc atttggaact tctgaacaca catttaagtt
     8221 agaaggcaaa ttgccaataa atgtcggtgc aatcgtgtca tcaacatgaa taaactgaac
     8281 agcacttgat gtattaccac attcatcagt tgcagtccaa gtacgtgtaa taataaattt
     8341 attaatacat gtttggtttg aaacttcatc gttaacactt atagaaattt cactagtaca
     8401 attgtctgcc acatcgacat ctccaatgct agcaactggt acatcatttg cacaacttag
     8461 tataatatct tctggaactc ctgtaataat tggacttata gaatcataaa caataatatt
     8521 ttgagacgca gtactagtat taccacaaat atcagtagct ttccaaactc tttttaatac
     8581 atatttattc gcacaactat ctggtttaat gaaatcttct acagtgattt gaacttcacc
     8641 agagcaatta tctgttgcgc ttacattttc tatgctcgct gctggtactt cgctcgcgca
     8701 tgctaatgta atatcttccg gtatttctat aaacactgga cttatagagt cattcactct
     8761 tattgtttgt tgcgaggtac tactattgcc acattcatct gttgctgtcc atgtacgt
//
LOCUS       DAOQQV010000109         8742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3091_length_8742_cov_6.494532, whole genome shotgun sequence.
ACCESSION   DAOQQV010000109 DAOQQV010000000
VERSION     DAOQQV010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_8742_cov_6.494532"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLL59_10145"
     CDS             complement(<1..231)
                     /locus_tag="PLL59_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24210.1"
                     /translation="MKKISLLSILILIIVFTFSYCNRKANASNGNSGTERTAQLVTLS
                     KNGITLTELYSPETFTDATLLQSTNAGATELDS"
     gene            complement(254..1735)
                     /locus_tag="PLL59_10150"
     CDS             complement(254..1735)
                     /locus_tag="PLL59_10150"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HMY24211.1"
                     /translation="MISIQLTYQHKKYWKKQGHISILGNIIIEDKFLAIEKQFEFFQL
                     IQNESTFSDAIKKVDGHFSILMETSENIFIAVDAIRTFPIFIKQKENDIYICDHIVPN
                     NDTLNIQASADFEKIYCTQENDTLLNDWHQIRAGEYVVINKEQHSVQFKSYFKHSEKT
                     SATNIKDAERKMIANAIAYVNNRTILIPLSGGYDSRYLLALLIENAYTKIECFTYGKQ
                     DTFEVNTAKKVCEQLNIKWHFIEYTDELLQSFFSASWTEYSMMNHHFTSLPHEQDFFA
                     LHYLKNKNALPKDTVVMNGFCQDLLAGSYLEPINNFKISDYVYEKYKVQIDTEKYDNT
                     WLGFQDWFTKNRLSKFIINSVRVYEYFDLDFYLPFWSKEWVSFWNNKSYKERYRQAFY
                     HQYLFDGLFKKYNIGFKKTATEQRGRWYYFKKIGKNILPDSFVKYIQLQNSKNQNKDV
                     NNTLYLYNKIYEQVKDKTTEKDFRINNIHARYFLQTLKNKFNL"
     gene            complement(1728..2933)
                     /locus_tag="PLL59_10155"
     CDS             complement(1728..2933)
                     /locus_tag="PLL59_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMY24212.1"
                     /translation="MKASTTSKNLFLNFSTLFSGLLVVQIINFAFSLLLPKYCSPFDF
                     AEFGIFTAIVFILIEIVNAKLDIAVMLGKDENKAIEILHAATTSAFVFTTVLLLIMLL
                     FYLIIPSIYFLLPFVILMYGIHQPIIVLWNKKETYSHINTFRILQVLSTSISTILLAM
                     NTISHALIIGFCIGIIVATSYCIYFYFPKIQWATTKNIWKKFDQFPKYGTWSALCNNF
                     SRNSVPILLSAFFPANWVGFYAYATRLLNAPSGMYTSALGQLYFKKASELTLIELKKL
                     TQKISYISIFVSIVPTLIILFFGKEIFHFLFGNEWTEAGRVAQCLILWYFVGMIITPI
                     STILDIKNKLNEEFKYNFSLLIVRIMSILIGGIFGNFYFTIILFSCVSILLNLGLLYY
                     IHTRLLQHD"
     gene            complement(2974..3510)
                     /locus_tag="PLL59_10160"
     CDS             complement(2974..3510)
                     /locus_tag="PLL59_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HMY24213.1"
                     /translation="MRQTILFILFFCSIFVLKAEEKEPYLVKTKNFHVQNFSIKKIDL
                     GITAVIYNPYKAKVTIDEILIDVFIGDKKLGTITEAAEDVKIKKESAFDLPLQIQVNT
                     GSTVAKFFTEGMKMLLLGKSVKVDYKGYIKVKALGFIPVKVKIDQSAYFTAKDILNDG
                     DEKPANTNTKDTTKTKTK"
     gene            complement(3520..4047)
                     /locus_tag="PLL59_10165"
     CDS             complement(3520..4047)
                     /locus_tag="PLL59_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24214.1"
                     /translation="MKNIIIVLFATLLFTACSEEKKDIEFKKMVNVHLGNLEKENAKL
                     NATAIFMNLSDQEYDLKDMTLDFTIDGKDVGTVVTKISKKIKANSEFSIPIQYTYETS
                     AIIEEGHDPSTTYAIQLLGNLTVKNTSGEEISAPVKFASTYEFLTKKEERIEQRKDKK
                     EERKKRREERKANKD"
     gene            complement(4133..4936)
                     /locus_tag="PLL59_10170"
     CDS             complement(4133..4936)
                     /locus_tag="PLL59_10170"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMY24215.1"
                     /translation="MKILNKIFLFFYFISTTSVALAYEVNADGIVLPTNPPRLVNDFI
                     NLLTTEQQAEIENELVAYNDSTSTQICVVIIDTVQGKNIEEYALQVGRIWGIGQKDKD
                     NGVLLLISKNDKKVDIEVGYGLEAHLTDYACRSIIQTSIVPNFKENNYYLGIKSAIYS
                     IKSYLDAEPISADYTYSLPQTKENWFEKYGYWLIVLIVFAIPLFVGFYFGIKHGFKGT
                     ISASSGGWSSSSSSSYSSSSSTYSSSSSSRSFGGFGGGSFGGGGSSGSW"
     gene            complement(4946..5707)
                     /locus_tag="PLL59_10175"
     CDS             complement(4946..5707)
                     /locus_tag="PLL59_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMY24216.1"
                     /translation="MTSLLNKYLFFILLLTQLTLQAQVVDKDGIPEKPSPPRLMNDYA
                     EMLSITEREQIEQKLVAYNDSTSTQICVVTVNTIGDYSIADYAVRLGRKWGIGQKDKD
                     NGILITISKLDRKVDIEIGYGLESHITDYDSKEIIDDIIIPAFKQNNFYEGVNKATDR
                     IISQLQGTFTASTSKKEQDDFNNIMVFIFVALIFIIIISSLFSSGKGNVTVSGGGWNW
                     SSGGGSSSWSGGGSSSSFGGFGGGSFGGGGSSGSW"
     gene            complement(5688..6128)
                     /locus_tag="PLL59_10180"
     CDS             complement(5688..6128)
                     /locus_tag="PLL59_10180"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMY24217.1"
                     /translation="MASAKNFLKDDEKNAVVAAIRQVELHTSGEIRVHIDDRCEQDAY
                     QRAVTIFEHLNMDKMPFRNAVLIYIAVKDRKFSIIGDEAIHEKLGDGFWKEISRELHN
                     DFENYNFSIGIINTIETIGKELIKYFPNIDELDRNELPDDISFE"
     gene            complement(6130..6708)
                     /locus_tag="PLL59_10185"
     CDS             complement(6130..6708)
                     /locus_tag="PLL59_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMY24218.1"
                     /translation="MKKSIYLLLLIVSTLLLPSCSYNSLVSHDEAAKKAWADVEAAYQ
                     RRSDLIGNLVETVKGEAKFEKETLVAVVDARSKATSIQLKADEITPENMAKFQEAQRQ
                     LSGALSRLLVVAEQYPNLKATEAFQNLMNELERTENRINIARRDYNEAVQNYNTQVRS
                     FPSNITANLFKFKQREPFKADPGTEKAPKVQF"
     gene            6866..7537
                     /locus_tag="PLL59_10190"
     CDS             6866..7537
                     /locus_tag="PLL59_10190"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HMY24219.1"
                     /translation="MAFEQEIELIRTKLNQYKKEGKTMFAGSSFQTHSIPMLHILSQI
                     DKTIPIYFLNTGYLFPQTIEYKDEIANLFGLTVIDQKSSTPRNMQKDSNGRLLFTSDP
                     DFCCHLNKIVPMEPILHSYAIWINGVRADQNANRAKMKEEQQTPQGAMRYHPMLHWTK
                     QMVWNYIKVNNIPHHPLDAQGYTSIGCEPCTRKIDSSQMGDERMARWFGLNKTECGLH
                     TELIK"
     gene            7556..8485
                     /locus_tag="PLL59_10195"
     CDS             7556..8485
                     /locus_tag="PLL59_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY24220.1"
                     /translation="MKVLITGGAGYLGTELVKKLVKIPKIKEIIVYDNLSKGAYNFFL
                     CTCIGGDNIRFVQGDILDTRKLKKELQGVDIVFNLAAKIATPYADADPHLYEQINHWG
                     TAELTYAIEESQVKKLIHISSLSVYGASSKGKLITENTKLNPRTNYGISKMRGEGFVE
                     RLLKNDKFETYIIRLGNVYGFNQTMRFDAVINLFAFEANFLKKISIHGDGQQIRSFIN
                     VDKSAQYITQLPFLNVPSGIYNMVDFNMSVMDIALAFKDLIPELEMQFINQHLQLRNI
                     SVDEDCKIYKYLPKQSTNFKEDLFNMMNQFAFH"
BASE COUNT         2757 a         1408 c         1396 g         3181 t
ORIGIN      
        1 gctgtctaat tctgttgcac ctgcattcgt actttgtaat aatgtagcat ctgtaaaagt
       61 ttcaggtgaa tataattcag ttaaagtgat gccatttttt gacaaggtta ctaactgagc
      121 agtgcgttct gttcctgaat tgccgttgct cgcatttgct tttctattgc aataggaaaa
      181 tgtaaaaaca attattaaaa ttaaaattga cagtaaggat atttttttca tgttgtaagt
      241 ttgagatgga taattataaa ttgaatttat ttttcaaagt ttgtaaaaaa tagcgtgcgt
      301 gaatgttatt tattctaaaa tctttttcag tagttttgtc tttaacttgt tcgtaaattt
      361 tattgtacaa atataaggtg ttattgacat ctttgttctg atttttgctg ttttgaagct
      421 gaatatattt tacaaatgaa tcaggcaaaa tatttttccc gattttttta aaatagtacc
      481 atctaccacg ctgttctgta gcagtttttt taaatccgat attatatttt ttaaacaagc
      541 catcaaataa atattgatga taaaacgctt gtctatatct ttctttgtat gatttgttat
      601 tccaaaagct gacccattcc ttactccaaa atggtaaata aaaatctaaa tcaaaatatt
      661 catatactct aacggagttg atgatgaatt ttgaaagcct gtttttggta aaccaatctt
      721 gaaaaccaag ccatgtattg tcatattttt ccgtgtcgat ttgcacttta tatttttcat
      781 atacataatc gctaatcttg aaattattta ttggctctaa gtaacttcct gccaataaat
      841 cttgacaaaa tccattcatt accactgtgt cttttggcaa tgcattttta ttttttaaat
      901 aatgcaaagc aaaaaaatct tgctcatgtg gcaatgatgt gaaatgatgg ttcatcatcg
      961 agtattcagt ccatgatgct gaaaaaaaag actgtaagag ctcgtctgta tattcgataa
     1021 aatgccattt tatattcaat tgttcgcaaa cttttttcgc tgtattgact tcgaaagtat
     1081 cttgttttcc ataagtaaaa cattcaattt tggtatatgc attttcgata agcaatgcca
     1141 ataaatatct cgagtcatag ccacctgaaa gtggaattaa aattgttcta ttattcacat
     1201 atgcaattgc atttgctatc atttttcgtt cagcatcttt tatgtttgta gctgatgttt
     1261 tttcgctatg tttaaaatag gatttaaatt gaactgagtg ttgttctttg ttaatgacaa
     1321 catattcacc agctctaatt tggtgccaat catttagtaa tgtatcattt tcttgtgtgc
     1381 aatatatttt ttcaaaatct gctgatgctt gtatatttaa tgtgtcatta tttggaacaa
     1441 tatggtcaca tatataaata tcattttctt tttgttttat aaaaatcgga aatgtacgaa
     1501 tagcgtcaac tgcgataaaa atattctcgc tggtttccat caatatagaa aaatggccat
     1561 ctactttttt aattgcatct gaaaaagtcg attcgttttg tatgagttga aaaaactcga
     1621 attgcttttc aatcgctaaa aatttatctt caataatgat gttgccaagt atagaaatat
     1681 gaccttgttt cttccaatat tttttatgtt gatatgtaag ttgaatgcta atcatgttgc
     1741 aataaacgag tgtgtatgta atacaacaaa ccgagattta ataagatgct tacgcaagaa
     1801 aaaagaatga ttgtaaaata aaaattacca aagattccac caattaatat actcatgatt
     1861 ctaacaatta ataaagagaa gttgtattta aattcttcat ttaacttgtt tttgatgtct
     1921 aaaattgttg aaattggtgt gattatcata ccgacaaaat accaaagaat caggcattgc
     1981 gcaacacgac ctgcttctgt ccattcattg ccaaataaaa aatgaaatat ttcttttcca
     2041 aaaaatagaa ttattaatgt tggtacgatg gaaacgaata tgctgatata gcttattttt
     2101 tgtgttagtt tttttaattc aattaatgta agttcgcttg cttttttaaa atataattgt
     2161 cctaatgccg atgtgtacat gccacttggc gcatttagta atcgcgtagc ataagcataa
     2221 aagccaaccc aatttgccgg aaaaaatgca ctcaataaaa ttggaacact gttgcgcgaa
     2281 aaattgttac acaatgctga ccatgtgcca tactttggaa attggtcaaa cttcttccat
     2341 atattttttg ttgttgccca ttgtattttt ggaaaataaa aatagatgca atagcttgtt
     2401 gcaacaataa tgccgataca gaaaccaata attaatgcgt gcgaaattgt gttcatggcc
     2461 aacaaaatag tgctaatgct ggtactgagt acttgcaata ttcgaaatgt attaatatgg
     2521 ctatatgttt cttttttatt ccaaagtaca attattggtt ggtgaatgcc atacattaaa
     2581 atgacaaaag gcagtaaaaa atagatactt ggaatgatta ggtaaaatag caacataata
     2641 agaagcaata ctgttgtgaa aacaaatgcg cttgtcgttg ccgcgtgtag tatttctatt
     2701 gctttgtttt catcttttcc aagcattacg gcaatgtcta atttcgcatt tacgatttcg
     2761 atgaggataa aaacaatagc tgtgaagatt ccaaattctg caaagtcaaa aggagagcag
     2821 tatttcggaa gtaataagga aaatgcgaag ttgattattt gtacgacaag caaccctgaa
     2881 aataaagtac tgaaatttaa aaacagattt ttgcttgtcg tgctagcttt catttatgct
     2941 aaagttacac attatttttt agcaataaat gagctatttt gttttcgttt tggtagtgtc
     3001 tttggtattt gtatttgctg gtttttcatc tccatcgtta agtatgtcct tagctgtgaa
     3061 atacgcactt tggtctattt ttacttttac tggtatgaaa ccaagcgctt ttactttgat
     3121 atagccttta taatctactt ttacagattt gcccaatagc aacattttca ttccttctgt
     3181 aaaaaattta gccacagttg agcctgtatt tacttgtatt tgtaaaggca aatcgaatgc
     3241 actttctttt tttattttta catcttcggc agcttcagta atggtgccta attttttatc
     3301 accaataaaa acgtctatca atatttcatc aatagttact tttgctttat atggattata
     3361 aattacagca gtaatgccaa ggtctatttt ctttatagaa aaattttgaa cgtggaaatt
     3421 ttttgttttt actagatacg gttctttctc ttccgctttt aatacaaata ttgaacagaa
     3481 aaataatata aataatattg tttgtctcat gctgaatgat tagtctttat tggcttttcg
     3541 ctcttctctt cgttttttac gttcttcttt tttgtccttg cgttgctcta ttctctcttc
     3601 tttttttgta agaaattcat aagtagatgc aaacttaaca ggagcgctaa tttcttctcc
     3661 tgatgtgttt tttacagtta aatttccaag aagttgaatc gcatacgttg ttgaaggatc
     3721 atgtccttct tcgatgattg cactggtttc ataagtatat tgaatcggaa tggaaaactc
     3781 agaatttgct tttatttttt ttgaaatttt agttaccaca gtgccaacgt ctttcccgtc
     3841 gatagtaaag tccaaggtca tatcttttaa gtcgtattct tgatcgctta agttcataaa
     3901 tatggcagta gcatttagtt ttgcattttc tttttctaaa tttcctaaat gcacattcac
     3961 cattttttta aattcaatat cctttttttc ttcagaacat gcagtgaaaa gtaaggtagc
     4021 aaacaatacg ataattatat ttttcataaa caaatttttg caaatgtatc taattgcacg
     4081 atagcatttt taattttaag aattttttac aaatatttat atagcaaaaa gcttaccaag
     4141 atccggagct gcctccaccg ccaaagctac caccaccaaa accaccaaag cttctgctcg
     4201 atgagctgct ggaataagta ctgctactac tggaataact actgcttgaa ctcgatgacc
     4261 aaccaccgct acttgctgat attgtacctt taaagccatg ctttattccg aagtaaaatc
     4321 caacaaataa tggtattgca aatacaatta aaacaatcag ccaatatcca tatttttcaa
     4381 accaattttc ttttgtttgt ggaagcgaat aggtataatc cgctgatatc ggttctgcat
     4441 ctaaataact tttaatacta tatatagcag atttgattcc tagataataa ttgttttctt
     4501 tgaaattagg aacaatactt gtttgtatta ttgagcgaca tgcataatct gttagatgcg
     4561 cttctaaacc atagcctact tctatatcta cttttttgtc attttttgat atcagcaaaa
     4621 gcacgccatt gtctttgtct ttttggccaa tgccccatat tcttccgact tgtagagcat
     4681 attcttcgat gttttttcct tgcactgtat caataataac aacacatatt tgtgtgctcg
     4741 tcgaatcatt gtaagccact aattcatttt cgatttcagc ttgctgttct gtagtcagta
     4801 aattaataaa atcattgact aagcgtggtg gattagttgg caacacaatg ccgtcagcat
     4861 tgacttcata agcaagtgct actgaagtag tgctaataaa atagaagaaa agaaatattt
     4921 tattcaggat tttcatcgtt tttatttacc aacttcctga gctgccgcct ccgccaaaac
     4981 ttccaccacc aaaaccaccg aaactactac tagagccacc gccactccat gagctactgc
     5041 cgccgccact actccaattc cagccaccac cacttaccgt aacatttcct tttccagagc
     5101 tgaataaaga agaaataata atgatgaata ttagagcaac gaaaataaac accataatat
     5161 tgttaaaatc atcttgctct tttttagatg ttgatgcagt aaatgtgcct tgcagctgac
     5221 tgataattct gtcagttgct ttattgacgc cttcatagaa attattctgt ttaaatgcgg
     5281 gaataataat atcatctatg atttctttag agtcgtagtc tgtaatatgg ctttctaaac
     5341 catatccaat ttcgatatct acttttcggt ctaattttga aatggtaatg agaatgccat
     5401 tgtccttatc tttttgtcca attccccatt ttctgcctaa ccgaacggca taatcagcaa
     5461 tactataatc gccaatagta tttactgtta ctacgcaaat ctgcgtagat gtagagtcgt
     5521 tgtatgctac taatttttgt tctatttgct cgcgttcagt aatgcttagc atttctgcat
     5581 aatcattcat cagtcgagga ggagatggtt tttctggaat gccatctttg tctactactt
     5641 gtgcctgcaa cgttagctgc gttaacaaga gtataaaaaa taagtattta ttcaaaagag
     5701 atgtcatctg gcaattcatt tctatctaat tcatcgatgt ttggaaaata ttttattaat
     5761 tcttttccaa tggtttcgat tgtattgatg ataccaatac taaaattata attttcaaaa
     5821 tcgttgtgta gttccctcga aatttctttc caaaagccat cgccgagttt ttcgtgtatg
     5881 gcttcgtcgc cgatgataga aaattttctg tcttttacag caatataaat caatacggcg
     5941 tttcgaaatg gcattttatc catattcaaa tgctcaaaaa tagttactgc acgttggtag
     6001 gcatcttgtt cacaacggtc gtcaatatgc acacgaattt cgccgcttgt atgtaattct
     6061 acttgacgaa tggctgcaac tacagcattt ttttcatcat cttttaaaaa gttttttgca
     6121 gatgccatgt tagaattgga ctttaggtgc tttttcagtt cctggatcag ccttaaatgg
     6181 ttcgcgttgt ttaaacttga aaagatttgc tgttatgtta gaaggaaagg agcgtacttg
     6241 tgtattgtaa ttttgaacag cttcgttata atctcttctt gcaatattta ttctgttttc
     6301 agttctttct agttcattca ttagattttg gaacgcttct gttgctttta aatttggata
     6361 ttgctctgca actaccaata aacgcgataa ggcaccacta agttgtcttt gtgcttcttg
     6421 gaatttagcc atattttctg gtgttatttc gtctgcttta agctgtatag aagtcgcttt
     6481 tgaacgagca tcaacaacgg ccactaatgt ttctttttca aacttagctt cacctttaac
     6541 cgtttcaact aaattgccaa ttaaatcaga tctacgttgg tatgctgctt ccacatctgc
     6601 ccaggctttt tttgctgctt catcgtgcga aactaagcta ttataggaac aacttggcaa
     6661 taataatgtg ctgacgataa ggagtaaaag gtaaattgat tttttcattt gttttatttt
     6721 ttaagtttga ataaagataa acataatttt gttttttttg gttccattta aaaattcaat
     6781 tgttttatag ctcaaactgc aacgctaatg atttattaat acttttttcg tcttcatatt
     6841 tattttaaga gtttattttt agtgtatggc atttgaacag gaaattgagc ttattcgaac
     6901 taagttaaat cagtataaga aagaaggcaa aacgatgttt gctggttctt catttcagac
     6961 acattctata ccgatgttgc atattttatc tcaaatagat aaaacgattc caatttattt
     7021 tttaaataca ggttatctat ttccacaaac gatagaatat aaagatgaaa ttgcaaattt
     7081 atttggacta accgttattg accaaaagtc ttcaacacca agaaatatgc aaaaggatag
     7141 caatggtaga ttgttgttta cttctgaccc agatttttgt tgccatttga ataagattgt
     7201 acccatggag cccatattgc atagttatgc tatttggata aatggcgtac gtgccgatca
     7261 aaatgcaaac cgtgcaaaaa tgaaagagga gcaacaaaca ccacaaggtg caatgcgcta
     7321 tcatccgatg ttacattgga caaaacaaat ggtttggaat tatattaaag tcaataatat
     7381 tccgcatcat cctttagacg cacaaggata cacgagtata ggttgtgagc cttgtactcg
     7441 aaaaattgat tcctcacaaa tgggcgacga acgcatggca aggtggtttg gcttaaataa
     7501 aactgaatgt ggattgcata cagaattaat taaataatta aacacaaaaa ataaaatgaa
     7561 agtattaatc actggtggtg ctggttattt aggaacagag ttggtaaaaa agcttgtaaa
     7621 aattcctaaa ataaaggaaa taatcgtgta tgataattta agtaaaggtg cttataactt
     7681 ctttttatgt acttgtattg gcggtgataa tatccgtttt gtccaaggtg atattttaga
     7741 tactcgaaag ctaaaaaaag aattacaagg cgtagatatc gtgtttaatt tagcggcaaa
     7801 aattgctaca ccgtacgcag atgcagaccc acatttatac gagcaaataa atcattgggg
     7861 aacggctgag ctaacttatg caattgaaga aagccaagtg aaaaagctta tccatattag
     7921 tagtttgtct gtttatggag catcatcgaa aggaaaatta attacagaaa atacgaagct
     7981 gaatccaaga acaaactatg gcatctcgaa aatgcgtggt gaaggatttg tagaacgttt
     8041 attgaaaaac gacaagtttg aaacttatat tattcgttta ggcaatgtgt atggatttaa
     8101 tcaaacaatg cgttttgatg ccgtaattaa tctttttgca tttgaggcaa actttttaaa
     8161 gaagatttcg atacatggcg atggccaaca aattcgttct tttattaatg tagataaatc
     8221 agcgcaatat attacgcaat tgccattttt aaacgtgcct tctggcattt ataatatggt
     8281 tgattttaat atgtcagtga tggatatcgc attggcattt aaagatttga ttcctgaatt
     8341 agaaatgcaa tttatcaatc aacacctaca attaagaaac atttctgtcg atgaagattg
     8401 taaaatatat aaatatctac ctaaacaatc tactaatttt aaagaagatt tgtttaatat
     8461 gatgaatcaa tttgcttttc attaatattt taacataagc atcttaaaaa gtacatatta
     8521 acttttatta tttttcaact tattgaaatt caattgttta ttattttttt tgcttaaata
     8581 aattattgtg gttacaagca tttatcaaca atgtagatat ttttttaggc tatcttaaaa
     8641 aaaaaatcaa aaaataattt ggagaataca aatagcttat taattttgtc gtagggtttt
     8701 tatgtttcta gactttaggg tctgacgggt gagtgcaaag ct
//
LOCUS       DAOQQV010000110         8426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3257_length_8426_cov_7.377016, whole genome shotgun sequence.
ACCESSION   DAOQQV010000110 DAOQQV010000000
VERSION     DAOQQV010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3257_length_8426_cov_7.377016"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLL59_10200"
     CDS             complement(<1..445)
                     /locus_tag="PLL59_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMY24221.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVDLLTPEEEVDLAQRIR
                     AGDQIALERLTKANLRFVVSVAKQYQNQGLTLSDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKVGSLNKINKAFSNLEQE"
     gene            578..2032
                     /locus_tag="PLL59_10205"
     CDS             578..2032
                     /locus_tag="PLL59_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMY24222.1"
                     /translation="MIKRVDRVVLGSFIVPLIITFGLTSFIFMMQFLWKYIDDFVGKG
                     LETSLLVKLVGLFTLTIVPMALPLAILLASTMVIGSLAEDNELTAYKTGGLSLLRIMG
                     SLIVFAIFLAGLTFYFSNNVLPKVHLRFYSLLYDIRKQKPALDIKEGIFYRGISGFAL
                     KIQHKDADNKTLYGIMVYDHSKGRGNDNVVLADKGDIALSNTGEHLILTLYNGKQYQD
                     VTKRSMPGNTEEPEQTVMEFKKWRKVFDLKEFKMTRTDQSLFKTHFIMYNAKQLMKEA
                     DSSRKDIENKIVEIQLNNGNYISYFKQIPDSVKLKNDTVTEKIDINNVFTRNIRESCL
                     STAKSNLQSLKSYTDMSIGDLEYKQSNYVNYYCEWYRKFSLSFACIVLLFVGCSMGAI
                     VRKGGFGVPILIAVLYFVLFHVLNVTGEKLAKGLIIPPFAGMWLSSLVLMPIALFLTY
                     KANRDSVLFRSEWYGYLFNKVKSIFILKKSLSKD"
     gene            complement(2127..2540)
                     /locus_tag="PLL59_10210"
     CDS             complement(2127..2540)
                     /locus_tag="PLL59_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HMY24223.1"
                     /translation="MYPEHLTKPCEQELTQAGFTALKSAEEVNNVLSATEGSTLVVIN
                     SVCGCAAANARPAVISSIQNSKRPTNLTTVFAGVDGEAVAKAREFMFPFPPSSPSIAL
                     FKDGKLVHFIERHMIEGRPVKMIADNLVAAYEAYC"
     gene            complement(2632..2964)
                     /locus_tag="PLL59_10215"
     CDS             complement(2632..2964)
                     /locus_tag="PLL59_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HMY24224.1"
                     /translation="MESEQLTKTFMEIQDEVYTQLQTIYDPEIPVNIFELGLIYEVNV
                     DIDNNVQVLMTLTAPNCPAAESLPAEVEQKVSAIEGVNSCKVDITFDPPWDQEMMSES
                     AKLELGFL"
     gene            complement(2977..3402)
                     /locus_tag="PLL59_10220"
     CDS             complement(2977..3402)
                     /locus_tag="PLL59_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HMY24225.1"
                     /translation="MKSINDIQNDIIEDFSMYDDWNDKYEYLIEFGKSLPLIDEQYKT
                     EENRIHGCQSRVWLHPELKAGKLYFYADSDAIITKGIVGLLVKVLSGHTPKDIYENEL
                     FFIEKIGLKEHLSPTRANGLVSMINKMKMYAFSYNEVNK"
     gene            complement(3421..4638)
                     /locus_tag="PLL59_10225"
     CDS             complement(3421..4638)
                     /locus_tag="PLL59_10225"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HMY24226.1"
                     /translation="MFNIQNIRADFPILNEKIRCNDLVYLDNGASTQKPSVVIEREKH
                     FYEHQYANIHRGVHFLSQIGTDLYEQTRQTIQHFIHAKHEHEIIFTKGTTNSINLVAY
                     SFGRTFIQEGDEIVVTEMEHHSNIVPWQILCAEKKCILKYIPLQSDGSISLEDAEKLI
                     TPKTKLVAVTYVSNALGTINPIEELIAIAHKNEAKILLDAAQAIQHFPIDVQALDVDF
                     LVFSGHKIYGPTGTGILYGKEEILNAMPPFEGGGDMIKTVSFKGTQFNDLPFKFEAGT
                     PNIAGCITLGTAIDYVQQIGLLEIKKYEEELLAYATEKLLTIDGLRIYGTAKNKSSVL
                     SFLLDGIHPYDVGVLLDNQGIAIRTGHHCAQPVMDYYGIPGTCRATIAMYNTKEEIDA
                     LYNGIVKAKKMLS"
     gene            complement(4654..5886)
                     /gene="sufD"
                     /locus_tag="PLL59_10230"
     CDS             complement(4654..5886)
                     /gene="sufD"
                     /locus_tag="PLL59_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HMY24227.1"
                     /translation="MNLDTDILEKILPTIKNSHQQHSLQNAINIGFPTMKQEEWKYTY
                     YNKILQQGFSFKTAEHTQTSSITKEFIDANATSSNRIVFINGIFHNEFSSLVNHPKIN
                     FWNSNNATESNTAEFLVEINNALSTDGIFLEIGDHVIVDEPIEIFYLSDNNQAIINLK
                     NKIIVGKNAQVKFAEYAINTQQSATYNNIVTSINVAENANVEFYKIQDGGAGFYSTDN
                     TFVHQAAKSVCNITTLSLSGEIIRNVLQFDINGERAAAHMNGLYLLQQNEHVDNRTLV
                     NHNVANCESFELYKGILGGTSTGVFNGKIVVKQAAQKTNAFQSNRNLMLSNDANIYTK
                     PQLEIFADDVKCSHGATSSQIEDSELFYLQARGIGKTTARGLLVFAFGEEIVEMIKPE
                     ALKIRMEKRIAELLNIEF"
     gene            complement(5978..6724)
                     /gene="sufC"
                     /locus_tag="PLL59_10235"
     CDS             complement(5978..6724)
                     /gene="sufC"
                     /locus_tag="PLL59_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HMY24228.1"
                     /translation="MLKINNLHVSIDNKEILKGFSLEVNKGEIHAIMGPNGTGKSTLG
                     NVLAGKEGYDITEGTIEFDGKNLLELEAHERAQAGMFLAFQYPIEIPGVSNANFLKAS
                     INEIRKSKGEEPMPPNEFLKMMREKMELVEMKKDFISRAVNQGFSGGEKKRNEIFQMA
                     MLNPKLAVLDETDSGLDIDALRIVANGVNKLHNSENAFIVITHYQRLLDYIIPDFVHV
                     MYDGRIVKTGDKSLALELEEKGYEEFELIK"
     gene            complement(6835..8280)
                     /gene="sufB"
                     /locus_tag="PLL59_10240"
     CDS             complement(6835..8280)
                     /gene="sufB"
                     /locus_tag="PLL59_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HMY24229.1"
                     /translation="MNEQDAILDEVVNSEYKYGFVTKIEQEQAPKGLNEDIIRFISAK
                     KEEPEWMLEWRLKAYREWLKMTEPKWANVHYPKVDFQDVIYYSAPKPKKELSSLDEVD
                     PELLKTFEKLGISLDEQKRLSGVAVDVVFDSVSVKTTFQEALKERGIIFCSMSEAIRN
                     HSDLVKKYIGTVVPQKDNFYAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINSAGSG
                     QFERTLLIADEGAYVSYLEGCTAPQRDENQLHAAVVELIALDNAEIKYSTVQNWYPGD
                     KEGKGGIYNFVTKRGICKGNNAKISWTQVETGSAVTWKYPSCILAGDNSIGEFYSVAV
                     TNHFQQADTGTKMIHIGKNTKSRIVSKGISAGKSQNSYRGLVKIHKKAENARNFSQCD
                     SLLMGDQCGAHTFPYLEVSNNNSKVEHEATTSKIGEDMLFYFQQRGIDEETAVAMIVN
                     GFCKEVMDHLPMEFAVEARKLLEISLEGSVG"
BASE COUNT         2476 a         1455 c         1365 g         3130 t
ORIGIN      
        1 attcttgttc taagttcgaa aatgctttat tgattttatt taatgaacct actttattaa
       61 gcggcaaacg cacaatacga gattgttcag ctaatgcctg taatatagat tggcgaatcc
      121 accaaaccgc ataagaaata aatttaaaac cacgtgtttc atcaaaacgt tgtgccgctt
      181 ttatcaaacc taaattacct tcgttaatta agtcactaag tgttaaacct tgattttggt
      241 attgcttagc tacagaaact acgaaacgca agttggcctt tgtcaatctt tctaatgcaa
      301 tttgatcgcc ggcgcgaatg cgttgtgcca gatccacttc ttcttcagga gttaataaat
      361 ctacttttcc gatttcttga agatattttt ctaaggattg gctttctctg ttggtgatgg
      421 atttggtaat ctttagctga cgcatacttt atctattgga ttgaatttta aaagagtgca
      481 aagataacat tatttcgttt gaaagtcaat atcttaatat tagattaaca tgatgttttt
      541 gtaatttaat gcctatttta gcaacagcat ttttcccatg attaaaagag ttgacagagt
      601 cgttttaggt agtttcatcg tgccgcttat catcacattt gggctcactt cttttatttt
      661 catgatgcag tttttatgga aatacatcga tgattttgtt ggtaaaggtt tggaaacgag
      721 cttattggta aagcttgttg gattatttac tttaactatt gttcccatgg cattgccatt
      781 agccatctta ttggcatcta caatggtcat cggttcttta gcagaagata acgagcttac
      841 cgcttacaaa acgggtggct tgtccttgtt gcgcattatg ggcagtttga tagtttttgc
      901 tatattctta gctggactta ctttttattt ttcaaataat gtattgccaa aagtacattt
      961 acgcttttat tctttattgt atgacatcag aaaacaaaaa cctgcattag atatcaaaga
     1021 gggcattttt tatagaggca tttcaggatt tgcactcaaa attcaacata aagatgccga
     1081 caataaaacg ctatatggta tcatggtgta cgaccattct aaaggcagag gaaatgacaa
     1141 tgtagtttta gcagataaag gtgatatcgc acttagcaac acaggcgagc atttgatttt
     1201 gaccttgtac aatggcaagc aatatcaaga tgtaacgaaa cgcagcatgc caggaaacac
     1261 ggaggagcca gagcagacag tcatggaatt taaaaaatgg cgtaaggtct ttgatttgaa
     1321 agaatttaaa atgacaagga cagaccaatc tttgtttaag acgcatttta ttatgtataa
     1381 tgcaaaacaa ttaatgaaag aagcagattc gagcaggaag gatattgaaa ataagattgt
     1441 tgaaatccaa ttaaacaacg gaaattatat ttcctatttt aagcaaatac cagactctgt
     1501 gaagctgaaa aatgatacag ttaccgaaaa aatcgacatc aataatgtgt ttacacgtaa
     1561 catacgagaa agttgccttt cgacagcaaa aagcaattta caaagtttaa aaagttacac
     1621 tgatatgtca attggcgatt tggaatataa acaatcaaat tatgtcaatt attattgcga
     1681 atggtacaga aaattcagct tatcctttgc gtgtattgtc ttgctttttg ttggctgttc
     1741 catgggtgca attgttcgca aaggtggttt tggtgttcct atattaattg cagttttgta
     1801 ttttgtacta tttcacgttt taaatgttac tggagaaaaa ttagcgaaag gtttaattat
     1861 tccaccattt gctggtatgt ggttgtcttc cttagtattg atgcctattg ccttattttt
     1921 aacttataaa gccaaccgcg attctgtttt atttagaagc gaatggtatg gctatttgtt
     1981 taataaagtg aaatctatat tcattttgaa aaaaagcctc agcaaagact aaagaattat
     2041 ttttccttgt aagaatacat aaaaaagaat ccatctttta taagaaaaag atggattcca
     2101 ataagtttta aaaattatta gttgaattag caatatgctt cgtaagcagc aactaagttg
     2161 tcagcaatca tttttacagg gcgaccttca atcatgtggc gctcgatgaa atgtactaat
     2221 tttccatctt tgaataaagc gatgcttggc gagcttggag gaaatggaaa cataaactct
     2281 ctagcttttg ctactgcctc gccatcaacg cctgcaaata ctgtagttaa gttagtagga
     2341 cgtttgctgt tttgaataga agagataacg gcaggtcttg catttgcagc agcacagcca
     2401 caaacagaat taattacaac taaagtagaa ccttctgttg ctgataatac attatttact
     2461 tcttcagcag attttaatgc tgtaaaacca gcttgtgtta attcttgttc gcatggtttt
     2521 gttaagtgtt ctggatacat gtttttataa ttttataatt aagtaattca atatttatgt
     2581 gtgtaattca aaaagtatgc aatttatttt ttctgacaat ttgtctttta tttataaaaa
     2641 acctaattcc aattttgctg attcgctcat catctcttgg tcccatggtg gatcgaatgt
     2701 gatatcaact ttacaactat ttacaccttc aatggcgctt actttttgtt ctacttcggc
     2761 aggcaaactt tcagcagctg gacagtttgg tgctgtcaac gtcatcaata cttgcacgtt
     2821 gttgtctata tctacattta cttcataaat caaacctaat tcaaaaatat taacaggaat
     2881 ttctggatca taaatggttt gtagttgtgt atatacttcg tcttgtattt ccataaacgt
     2941 ctttgttaat tgttcgcttt ccatgttcat tttttattat ttattcactt cgttgtagct
     3001 aaatgcatac attttcattt tgtttatcat gctcactaaa ccattggcac gcgtaggaga
     3061 caagtgttct ttcaatccaa ttttttcgat aaaaaaaagc tcattttcat agatgtcttt
     3121 tggtgtgtgg ccacttaata cttttaccaa taaaccaaca attccttttg taataatggc
     3181 atcactatct gcataaaaat agagttttcc agctttcaat tcagggtgca accacactcg
     3241 agattggcag ccatgtattc tattttcttc cgttttatat tgctcgtcaa ttaatggtaa
     3301 ggattttcca aactcaatca gatattcgta tttatcgttc caatcatcgt acattgaaaa
     3361 atcttcaatg atatcgtttt gtatgtcgtt tattgatttc aatttttttt taattatgtt
     3421 ttatgatagc attttttttg cttttacaat gccattataa agtgcatcta tttcctcttt
     3481 tgtattgtac attgcaattg ttgctctgca tgttcctgga attccataat aatccattac
     3541 tggttgtgcg caatgatgtc ctgtgcgtat tgcgattcct tgattatcta ataaaacgcc
     3601 aacgtcataa ggatgaatgc catcgagtag aaatgacaac acagaagatt tgttttttgc
     3661 tgtaccgtaa attctcaaac catcaatagt caataacttt tctgttgcat atgccagtaa
     3721 ttcctcttcg tattttttta tttccaataa accaatttgt tgcacgtagt cgatagcagt
     3781 acctaaggta atgcagccag caatatttgg tgtgccagcc tcaaatttga aaggtaaatc
     3841 attaaattga gtgcctttaa aagatactgt ttttatcata tcacctccac cttcaaatgg
     3901 tggcattgcg tttaatattt cttcttttcc atataatata ccagtgcctg ttgggccata
     3961 tattttatgt ccgctaaaca ctagaaaatc tacatctaat gcttgtacat caattggaaa
     4021 atgttggatt gcctgtgcgg catctaataa aatttttgct tcatttttat gtgcaatcgc
     4081 tattaattcc tcaataggat tgatggtgcc aagtgcatta gatacgtaag taactgcaac
     4141 caattttgtt ttaggcgtaa tcaatttttc agcatcttct aatgaaatac tgccgtctga
     4201 ttgcaatgga atatatttta aaatacattt tttttcagca cacaaaattt gccatggaac
     4261 aatattggaa tgatgttcca tttctgttac tacaatttca tcgccttctt gaataaatgt
     4321 tcttccgaac gaataagcca ccaaattaat agagttggta gtgccttttg tgaaaattat
     4381 ttcatgttca tgtttggcat gaataaaatg ttgaatcgtt tgtcgtgttt gttcgtataa
     4441 gtctgttcct atttgcgata agaaatgcac accacgatga atgttagcat attgatgttc
     4501 ataaaaatgt ttttctcttt caataaccac agatggtttt tgtgtagatg cgccattatc
     4561 gagatatacc aagtcattac aacgaatttt ttcgtttaat atgggaaagt cggcgcgtat
     4621 attttgtatg ttaaacatat tgaaaatgtt tgtctaaaat tctatattta gtaactctgc
     4681 aattcttttc tccattctga tttttaatgc ttctggttta atcatctcaa cgatttcttc
     4741 accaaatgca aaaaccaaca aaccgcgagc tgttgttttg ccaatgcctc ttgcttgtag
     4801 ataaaataat tcgctgtctt cgatttgtga tgatgttgcg ccatgcgagc actttacatc
     4861 atctgcaaaa atttctaatt gaggctttgt ataaatattc gcatcattgc taagcattaa
     4921 gtttctattg ctttgaaagg cgttcgtttt ttgtgctgct tgttttacca caattttccc
     4981 attaaaaact cctgtagatg tgccacctaa aatgccttta tataattcaa aactttcaca
     5041 gttagcaaca ttatggttga ccaaagttcg attgtcaacg tgttcatttt gttgtaataa
     5101 atacagacca ttcatgtgtg ctgctgcacg ttctccattg atatcgaatt gcaatacatt
     5161 tctaatgatt tcgccactca aagaaagcgt agtaatatta caaacagatt tggcagcttg
     5221 atgtacaaaa gtattgtctg tgctataaaa tccagcgcca ccatcttgta ttttatagaa
     5281 ttctacattg gcattttctg ccacattaat tgaagtaaca atattgttgt atgttgctga
     5341 ttgctgtgtg tttattgcat attcagcaaa ttttacttgt gcattttttc ctacaataat
     5401 tttgttttta agattgatga ttgcttgatt attatcactc aaataaaaaa tttcaatcgg
     5461 ttcatcaacg ataacatggt cgccaatttc aagaaaaata ccatcagtgc tgagtgcatt
     5521 atttatttct actaaaaatt ctgccgtgtt gctttctgtt gcgttgtttg aattccaaaa
     5581 gtttattttt gggtggttta ctaaagaaga aaactcgttg tggaaaatgc catttataaa
     5641 aactattcta ttgctggacg tcgcatttgc atcgatgaat tcttttgtaa ttgagcttgt
     5701 ttgtgtgtgc tcagcagtct taaacgaaaa accttgttgc agtattttat tataataagt
     5761 gtacttccat tcttcttgct tcattgtcgg aaagccaatg tttatagcat tttgcaaaga
     5821 atgttgttga tgactatttt taattgtagg caatattttc tctaatatat cagtgtctaa
     5881 attcattaat ttgtaatttg gtaattcgat aatgattttt gaaagtgtat gaaatttatt
     5941 tttaatatct atacattttc aattgctgat ttggtattta tttaattaat tcaaattcct
     6001 cgtatccttt ttcctctagc tccaaagcca atgatttatc gccagttttt acaattctac
     6061 catcatacat tacatgaaca aaatcaggaa taatatagtc gagcaatctt tggtaatgag
     6121 tgattacgat gaatgcattt tcactattgt gtaatttgtt tacaccattg gcaacaatac
     6181 gcaatgcatc gatatctaag ccagagtctg tctcatctaa gacggctaat tttggattaa
     6241 gcatagccat ttgaaaaatt tcatttcgtt ttttttcgcc accagaaaat ccttgattta
     6301 cagctcgcga aataaaatct tttttcattt ctactaattc cattttttca cgcatcattt
     6361 tcaaaaattc atttggtggc attggctctt cacctttaga ttttcgaatt tcatttatcg
     6421 atgcttttaa gaaattcgca ttgctcacgc ctggtatttc gattggatat tgaaatgcca
     6481 aaaacatacc agcttgtgca cgttcatgtg cttccaattc taataaattt ttgccatcaa
     6541 attctattgt accttctgta atgtcataac cttctttacc agcgagcaca ttgcctaatg
     6601 ttgatttgcc tgtaccgttt gggcccatga tagcatgtat ttcaccttta tttacttcaa
     6661 gtgaaaagcc tttaaggatt tctttattgt cgattgatac gtgtaagttg tttattttaa
     6721 gcataataat tattgttttt gacaatcgtc taattggata attagctgat atttttgttg
     6781 tttaattttt tttgtctttt atttatcgaa ttcgctaatt cgctaattgt ttcattatcc
     6841 tactgaacct tctaaactta tttctaataa ttttctagct tctacagcaa attccatggg
     6901 taaatgatcc attacttctt tacaaaagcc atttacaatc atggcaactg cagtttcttc
     6961 gtcgatgcca cgttgttgaa aatagaataa catatcttct ccaattttag atgtcgttgc
     7021 ttcgtgttca acttttgaat tgttattgct tacttctaga tagggaaacg tatgtgctcc
     7081 acattggtcg cccattaata aagaatcaca ttgtgaaaaa ttgcgcgcat tttcagcttt
     7141 tttatgaatt ttcaccaaac cacgatatga gttttgcgat tttccggcag aaataccttt
     7201 tgacacaatg cggctttttg tgtttttgcc tatgtgtatc atcttggttc ctgtgtctgc
     7261 ttgttggaaa tgattagtta cggctactga ataaaactcg ccgatactat tatcacctgc
     7321 taaaatacaa ctcggatatt tccatgtaac agctgagcct gtttctacct gtgtccacga
     7381 tatttttgcg ttatttcctt tacaaatacc acgcttggtt acaaaattat aaatgccacc
     7441 ttttccttct ttgtcgccag gataccaatt ttgaacagtg ctatatttga tttctgcatt
     7501 atctaaagca attaattcaa caacggctgc atgtagctga ttctcatcac gttgtggagc
     7561 cgtgcaacct tctaagtagg atacataagc gccttcgtca gcaatgagta aggtgcgttc
     7621 aaattgtcca gaacctgcag aatttattct gaaataagtg cttaattcca ttgggcaacg
     7681 tacgcctttt ggaatataac agaatgagcc atcactaaaa acggcactat ttaaagcagc
     7741 ataaaaatta tctttttgtg gtactacagt tccaatatat tttttgacta aatctgaatg
     7801 atttcgtata gcttcactca tcgagcagaa aattatacca cgttctttta atgcttcttg
     7861 gaatgttgtt ttcacagaaa cgctatcaaa aacaacgtca acagcaacac ctgacaacct
     7921 tttttgctca tctaatgaaa tgcctaattt ttcaaatgtt tttagtaatt ccggatcaac
     7981 ctcatctaaa gaagataact cttttttagg ttttggtgcc gaataataaa ttacgtcttg
     8041 gaaatctact tttggataat gtacatttgc ccatttgggt tctgtcattt tcaaccattc
     8101 tctgtaggct tttaatcgcc attccaacat ccattctggt tcttcttttt tggctgaaat
     8161 aaatctgatg atatcttcat ttaagccttt tggcgcttgt tcttgctcta ttttggttac
     8221 aaaaccgtat ttatattctg agtttacaac ttcatctaat atagcatctt gttcgttcat
     8281 acttaattta agctattagt acctaaaatt cggtacaaag ttattccgca tttaacttaa
     8341 aaacaagtat tatgtagaat cgttctaaat aaattcattt tttttatgtt aagtttttaa
     8401 aatgtatttt tgattactat gaaaaa
//
LOCUS       DAOQQV010000111         8417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3264_length_8417_cov_6.762258, whole genome shotgun sequence.
ACCESSION   DAOQQV010000111 DAOQQV010000000
VERSION     DAOQQV010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3264_length_8417_cov_6.762258"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLL59_10245"
     CDS             <1..350
                     /locus_tag="PLL59_10245"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622252.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HMY24230.1"
                     /translation="IREHNQDYWTGTVYTTNRRVWEHDEKFKEYLRTIRAMAIDMETA
                     TVFSVAFANEIPVGALLLVSDQPMIPEGVKTEESDKKITTNFVDLHLQIGIASLQQLM
                     NRTSTIKHLRFED"
     gene            complement(386..724)
                     /locus_tag="PLL59_10250"
     CDS             complement(386..724)
                     /locus_tag="PLL59_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24231.1"
                     /translation="MKALIQILFSIFFIIAIASHVYFVIVDDGQPFWWHAIYYITYGG
                     CWWMLQMHNKKALAMYIAFAIFPFVTHANIMFQHYPKLDFLFWIGFIVCIMLIFGVIW
                     IKKTIVETMV"
     gene            complement(724..1686)
                     /locus_tag="PLL59_10255"
     CDS             complement(724..1686)
                     /locus_tag="PLL59_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HMY24232.1"
                     /translation="MSIQKKSEKYKRLIRSEKFQDKLRNISIPGFEGIPIYDVFVKFK
                     EEIKNDDLSIRASSISFYFILALFPTIIFFFSLIPYVPITNFDEKIMHGLKSVLPINA
                     FIILESTIHNIVSRQHGGVLSINFFLALFVASNGVNSMMKTFDKMNHTFKTRNWWQKR
                     FAAIRILVLVCLQIIIAVLLIIKGNEFLAFLLRFIHAEDEHTLFVLHIVKLFLIVFSF
                     FNIIALIYYYGPSVKKKYRYFSVGATFATIISIIMTYGFQVYASYLNNFNRLFGSLGI
                     MIVIMMLIYLNALVLLFGFELNNSIAINKALRNNNDNHTNQDEG"
     gene            complement(1742..2215)
                     /locus_tag="PLL59_10260"
     CDS             complement(1742..2215)
                     /locus_tag="PLL59_10260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMY24233.1"
                     /translation="MFEETIQLRVRYHDCDPLGIVYHGHYAKFFEIGRTEAMRKHDFS
                     YKKLEELGYAMPVVEMNIKYFRPARYDELMNIKTIIKEWPDKSIVFIGEIYNQQHQLL
                     TSSETKFVYVKKDSLRRCHIPEKVLEHLTPFFTEELKKIKEEKKAAIKAKASHKI"
     gene            complement(2224..3261)
                     /gene="mltG"
                     /locus_tag="PLL59_10265"
     CDS             complement(2224..3261)
                     /gene="mltG"
                     /locus_tag="PLL59_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HMY24234.1"
                     /translation="MKKKISIFLIIILCLLLIAFIFTYKLLFADNINDNQTQKQHLFF
                     VHTGSSYNDVLLALKKQHLIKDMSTFKLLAEKLNYKQNVKPGRYTFDNSSGNLTILRK
                     LRSGSQEPVKITFNNIQNKEQFAEKIAKQIEATKEELLREFNNAQFLDSLQLTEENFP
                     TIFLANTYEFYWNTSARQFIDRMLKEYHKFWNEDRLEKAQAIGLSPTDVIILASIIQK
                     ESTHYDEYPTIAGVYLNRLKIGMPLQADPTVLFALKKLGVSRRVLHADLKIKSPYNTY
                     LNKGLPPGPICLPELKSIDETLQAEEHNYIYFCAKEDFSGYANFAETWEQHQANARKY
                     QQALNERNINR"
     gene            complement(3969..6830)
                     /gene="gcvP"
                     /locus_tag="PLL59_10270"
     CDS             complement(3969..6830)
                     /gene="gcvP"
                     /locus_tag="PLL59_10270"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HMY24235.1"
                     /translation="MQNEFQLRHIGPRPSDVEAMLQTIGVNSLTQLIEEAVPANIRMK
                     HTLSIGEPQTEFQYLQELKEKAAKNKVCKNYIGLGYYGTITPGVIQRNIFENPGWYTQ
                     YTPYQAEISQGRLEALLNFQTMISDLTGLPIANASLLDEGTAVAEAMHVLFEIRNKSK
                     NKPQAHKIFIDKHTLPQTIDVVVGRALPLNIEVVVDDYKNFTPSEAFFAAVVQYPNAS
                     GAIEDYTAFTTACNSQEVYTVFACDLLSLALLSSPGELGADIAVGNSQRFGVPMGFGG
                     PHAAFFACKEEFVRLIPGRLIGISVDKHGNRALRMALQTREQHIKREKATSNICTAQA
                     LLAIMASMYAVYHGKEGIKKIAQKTHNNAVALANELKKLGFGNKNTFFFDTLKIDSKG
                     KADTIKKIAENNAYNFWFNQDGTIQISLDETTTQADVEKVASIFAESIGANYVSSYLD
                     NPAVQIPENLQRTSDFLTHDVFNSHQSETEMLRYIKRLEAKDLSLCFSMIPLGSCTMK
                     LNATTELVPLSWPEFAHIHPFQPANQVQGFLEIIQELEAQLSNITGFAATSLQPNSGA
                     QGEYAGLMVIRAYHLAKGDTHRNVALIPTSAHGTNPASAAMAGMEIVLVKSTPEGNID
                     VEDLKVKAAQYAANLSCLMVTYPSTHGVFEETIIEICDTIHQYGGKVYMDGANMNAQV
                     GLTSPASIGADVNHINLHKTFAIPHGGGGPGMGPICCTSELAPYLPSNPLVKTGGANA
                     IPAISAAPFGSAAILLISYAYNKMLGSEGVTNSTKFAILNANYLKAKLEQHYPILYTG
                     ANGRVAHEFIMDIRPFKKDTGIDAADIAKRLMDYGYHAPTVAFPVPGTLMIEPTESES
                     KEELDKFCSALISIKKELDNISAQITSVADNPIINAPHVAAEVIASEWNHCYSRETAA
                     FPLEYVKTTKFWPSVAKIDNTYGDRNLVCTCLPTEAYAK"
     gene            complement(7013..>8417)
                     /locus_tag="PLL59_10275"
     CDS             complement(7013..>8417)
                     /locus_tag="PLL59_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HMY24236.1"
                     /translation="GITCSTNEMSAKGEHGMKIWLDKVPTRQKNMQPFEILLSESQER
                     MLIVVQKGKEKEVEAVFEKWDLHCAIIGEVTAGNLVQYYMHDTLVAEVPADTLVLGGG
                     APVYERSYKQPSYHAKRDEFILDDKVPFPKSYIDVAKMLVQHPNIASKRFVYKQYDSM
                     VGINNVSTNRPSDAAVIRLKDSKKGLVVTVDCNSRYVHADAYKGGAIAVSEAARNIVC
                     TGAKPVAITNCLNFGNPYDEEVYWNFVHALQGMGDACLQFETPVTGGNVSFYNQSPNR
                     AVNPTPTIGMLGIIEDVDNIMTLDFKQEGDLIYLIGESKNDIGSSEYLVQYHKVELSP
                     APYFDLQEEAKIQDAVASLIDKKLIQSAHDLSEGGLFIAVLESAMTRNFGFEINTCTT
                     IRKDACLFGEGQSRVVVSIRANEKENFETHLKSNNIPNQLIGTVKGNNIIVDGENYGT
                     IAEYKELYDTAIEKYFV"
BASE COUNT         2579 a         1459 c         1412 g         2967 t
ORIGIN      
        1 ctattcgtga gcacaatcaa gattattgga caggcactgt ttacacaact aatagacgtg
       61 tatgggagca tgatgaaaag tttaaagagt atttgagaac cattcgtgca atggcaatag
      121 atatggaaac ggcaactgta ttttctgttg catttgctaa cgaaattcct gttggcgcct
      181 tgttattagt gtcagaccaa cctatgattc ctgaaggcgt aaaaacagaa gaaagtgaca
      241 aaaaaattac gacaaatttt gttgatttac atttacaaat cggcatcgca tcactacaac
      301 aattaatgaa cagaaccagt acaataaaac atttacggtt tgaagactag aaaataatgt
      361 tccccaacat aaaaaccgtt tgtttttaaa ccattgtttc tacaatggtt ttttttatcc
      421 atatcactcc gaaaataagc ataatgcaaa caatgaatcc aatccaaaat aggaaatcta
      481 attttggata gtgttgaaac ataatatttg cgtgtgttac aaatggaaaa attgcaaatg
      541 ctatatacat agctaatgct tttttattgt gcatttgcaa catccaccaa catccaccat
      601 aagtaatgta atagattgcg tgccaccaaa atggctgtcc atcatctacg atgacaaaat
      661 atacatgtga tgcaattgcg ataataaaaa atattgaaaa taagatttga ataagtgctt
      721 tcattaacct tcatcttgat tagtatgatt atcgttgttg ttgcgcaacg ctttattgat
      781 ggcaatgcta ttatttaatt caaacccaaa aagcaacact agtgcattca gataaatcag
      841 catcatgatg actatcataa tacctaatga gccaaataat ctattgaaat tgttgagata
      901 agaagcatat acttgaaagc cataggtcat tataatcgaa atgatggtgg caaatgttgc
      961 gccgacagaa aaatagcgat atttcttttt tacagatgga ccataataat aaataagtgc
     1021 aatgatatta aagaagctaa acacgattaa aaataatttg actatatgta aaacaaacaa
     1081 agtatgctcg tcttcggcat gaataaatcg aagtagaaaa gccaaaaatt cattgccttt
     1141 aataatgagt aatactgcaa taattatttg caaacaaact aatacaagaa tacgaatagc
     1201 cgcaaatctt ttttgccacc aatttctcgt tttaaatgta tgattcattt tgtcgaatgt
     1261 tttcatcata gaatttacac cattcgacgc aacaaataag gctaaaaaga aatttattga
     1321 taatacacca ccgtgttgtc tgcttacaat attatgaatg gtactttcta aaattatgaa
     1381 tgcatttatt ggtaaaacgc tcttcaatcc atgcattatt ttttcatcga aatttgtgat
     1441 tggcacataa ggaatgagtg agaaaaagaa aatgatagta ggaaataagg ctagaataaa
     1501 ataaaaagaa atagaagatg ctcgaataga caaatcatca tttttaattt cttctttaaa
     1561 cttgacaaat acatcataaa tcggaatgcc ttcaaagcct ggaatactaa tgtttcttag
     1621 cttgtcctga aatttttcag aacgtattag tcgtttatat ttctcacttt ttttttgaat
     1681 agacatgaca acaaaactga ttttaaaatt agcaaatatt ctaaaatttt aatatcaaca
     1741 actaaatctt atgtgatgct tttgctttta tggctgcctt tttttcttct ttaattttct
     1801 ttaattcttc tgtaaaaaaa ggagtgaggt gttctaatac tttttctgga atatgacatc
     1861 tgcgaagtga atcttttttt acatatacaa atttagtttc gctactcgtc agtaattgat
     1921 gttgttgatt gtaaatttca ccaataaaaa caatgctttt gtcgggccat tcttttatta
     1981 tagtttttat attcatcaat tcatcatagc gagctggtct aaaatattta atattcattt
     2041 caacgactgg catagcatat ccaagttctt ccaatttctt gtacgaaaaa tcatgtttac
     2101 gcatggcctc agtcctgcca atttcgaaga attttgcata atgaccatga tatactattc
     2161 ctaaaggatc gcaatcatga tatcgcactc ttagttgtat tgtttcttca aacatgtctt
     2221 tgcttatcga ttgatgtttc tttcatttaa tgcttgctga tattttcttg cattagcttg
     2281 gtgttgttcc catgtttcag caaaatttgc atatcctgaa aaatcttctt tagcacaaaa
     2341 atatatataa ttatgttctt ctgcttgtaa agtttcatca atgctcttta attcgggcaa
     2401 gcaaattggt ccaggtggca aacctttgtt aagataggtg ttataaggag attttatttt
     2461 taaatcagca tgtagcacac gtctgcttac accaagtttt tttaaagcaa acaaaactgt
     2521 tgggtcagct tgaagaggca ttcctatttt caatctgttt aaataaacac cagcaatggt
     2581 tggatattca tcataatgtg tagattcctt ttgaataata gaagctaaaa tgattacatc
     2641 agtaggtgat aagccaatgg cttgtgcttt ttcgagccta tcttcattcc aaaatttatg
     2701 atactctttc agcattctgt caataaattg tctggcagaa gtattccaat aaaactcata
     2761 agtgtttgct aagaaaatag tcggaaagtt ttcttctgtc agttgcagcg aatcaaggaa
     2821 ttgagcatta ttaaactcac gcaacagttc ctctttagtc gcttcaattt gtttggcaat
     2881 tttttcggcg aattgctctt tgttttgaat attgttaaaa gtaattttta ctggttcttg
     2941 acttcctgaa cgcagttttc tcaaaatcgt taaattgcca cttgaattat cgaaagtata
     3001 tcttcctggt tttacgttct gtttgtaatt aagcttctct gcaagcaact taaatgtgct
     3061 catgtctttt ataagatgtt gttttttcag tgcaagcaat acatcattgt aagaagaacc
     3121 tgtgtgtacg aaaaataggt gttgtttttg tgtttgattg tcatttatat tatctgcaaa
     3181 taatagttta taggtgaaaa taaatgcaat taagagtaaa caaaggataa ttatcaagaa
     3241 aattgaaatt ttttttttca taagcataag gcgtaatcta ttgattttaa ttgtgttaca
     3301 aagatattga gaaaaataat taaaataaaa taaaaaaaag ttaaacaact agttgcgaaa
     3361 tacaaaaagt tgtttaactt tgcaataggg tttttatgtt tctagacttt agggtctttc
     3421 gggttttgtg caatgcaaaa cccgattttt tttgtctata cttttaacat cagcttttta
     3481 cagccaaata ccaataaata atgtaagaaa atctttcaga gaaatagtta gtttcatttt
     3541 gggtttttat gaaatataat aagttagttt agttccccga taagtgttct tacttcttat
     3601 ctgatacaaa gatgtaacta atattttcat cagaagtatc aactttattt tttatgtgac
     3661 taatgtttta agttatttat atttataagt ggttcattgt cagtatttat tatggttttt
     3721 tattattcat ttagtgactt tttttacacc gttttaacat tgaattctat tgaatttaat
     3781 cttgaattgg ctatgaaaat cgaaattaca tgccatttct ttttatttca ttgtatatat
     3841 tgacattgta atactcaact tttaccataa aaatataact gcctctgaaa aagaggcagc
     3901 caaattatat attttgaaat atttattctg ccgataattg ttttatcagc gaattcaagc
     3961 acttatgctt atttagcata ggcttccgtt ggcaaacaag tacatactaa attcctgtcg
     4021 ccataagtgt tgtctatttt agcaacagat ggccaaaatt ttgttgtttt tacgtattct
     4081 aaaggaaatg ctgctgtttc tctactgtag cagtgattcc attcgctagc aatgacttca
     4141 gccgctacat gtggtgcatt tataattgga ttatctgcta cactcgtaat ttgtgcactg
     4201 atattatcta attctttttt tatactgatt agtgcgctac agaatttatc taattcttct
     4261 ttgctctcgc tctcagttgg ttctatcatt aatgtacctg gcactggaaa tgcaactgtt
     4321 ggtgcatggt aaccatagtc cattaatctt tttgcaatat ctgctgcatc tatgccagta
     4381 tctttcttaa acggtctaat gtccataata aattcgtggg caacacgacc atttgcgcca
     4441 gtgtataata ttggatagtg ttgctctaat tttgctttta aataattagc atttaaaatc
     4501 gcaaatttag ttgaattagt gacaccctca ctgcctaaca ttttattgta ggcataagaa
     4561 atcaataata tggcagcact accgaatggc gcagcagaaa tagcaggaat tgcattggca
     4621 ccacctgttt ttactaaagg atttgatggt aaatatggtg ctaattctga tgtacaacaa
     4681 attggaccca tgcctggacc accgccgccg tgtggtatgg caaatgtttt atgtaaattg
     4741 atgtggttaa catcagcgcc aattgatgca ggcgaagtta agccaacttg tgcattcatg
     4801 tttgcaccat ccatatatac tttaccgccg tattggtgaa tggtatcaca tatttcaata
     4861 atagtctctt caaacacacc atgcgtagat ggataagtga ccattaagca agataagttt
     4921 gcagcatatt gcgctgcttt tacctttaga tcttctacgt caatattgcc ttctggcgtt
     4981 gattttacca atacaatttc catgcctgcc atagctgcgc tagcgggatt tgttccatgt
     5041 gcagatgttg gaattaaggc tacatttcga tgtgtgtcac cttttgctaa atgatatgca
     5101 cgaatcacca ttaatcctgc atactcgcct tgagcacctg agtttggttg tagtgaggta
     5161 gctgcaaatc cagtaatatt ggacaattga gcttctaatt cctgaatgat ttctagaaaa
     5221 ccttgtactt gatttgccgg ctgaaaagga tgtatgtgtg caaattctgg ccagcttaat
     5281 ggaactaatt ctgtcgtagc atttagtttc attgtgcagc ttcctaaagg aatcatagaa
     5341 aaacataatg acaaatcttt tgcttccaaa cgtttgatat aacgcaacat ctctgtctct
     5401 gattgatggg aattgaatac gtcatgtgtg agaaagtctg atgtacgttg taaattttct
     5461 ggaatttgta cagctggatt atctagatag ctcgaaacat aattcgcacc aattgattca
     5521 gcaaaaatgc ttgctacttt ttctacatcg gcttgtgtag ttgtttcgtc taagctaatt
     5581 tgaatggttc catcttggtt aaaccaaaaa ttatatgcat tgttttccgc aattttttta
     5641 attgtatctg cctttccttt gctatcaatt tttagtgtat caaagaaaaa tgtattctta
     5701 ttgccaaagc ccaatttttt taattcattg gctaatgcaa cagcattgtt atgtgttttt
     5761 tgtgcaattt tttttatgcc ttcttttcca tgatatacag catacatact tgccatgatg
     5821 gccaataatg cttgagctgt acagatgttt gatgtggctt tctcacgttt aatgtgttgc
     5881 tctcgtgttt gcaatgccat acgtaaagca cgattaccgt gtttgtccac agatatacca
     5941 atcaatcggc ctggaataag tcgaacgaat tcttctttac atgcaaaaaa tgctgcatgc
     6001 ggtccaccaa aacccatcgg cacaccaaat cgttgtgaat ttccgacagc aatgtctgca
     6061 cccaattcgc ctggtgatga caatagtgct aaacttagta aatcacatgc aaatactgta
     6121 tatacttctt gtgaattaca agcagtagta aatgcagtgt aatcttcaat agcgcccgat
     6181 gcattcggat attgaactac tgcagcaaag aatgcttcac ttggtgtaaa gtttttataa
     6241 tcatctacaa ctacttcaat atttaaaggt aatgcacggc ctacaaccac atctattgtt
     6301 tgcggcaaag tgtgtttgtc aatgaatatt ttatgcgctt gcggtttgtt ttttgattta
     6361 tttctgattt caaataatac gtgcattgcc tcggcaacag cagtgccttc gtctaataaa
     6421 gaggcatttg caataggcaa acccgtaagg tctgatatca ttgtttggaa gtttaataaa
     6481 gcctccaatc tgccttgtga aatttctgct tgataaggtg tgtattgtgt gtaccaacct
     6541 gggttttcga aaatattgcg ttgtataaca ccaggtgtaa tagtgccgta ataaccaagg
     6601 ccgatatagt ttttacaaac tttatttttt gcagctttct ctttcaattc ttgtaaatac
     6661 tgaaactcag tttgtggttc tcctatcgac aaagtgtgtt tcattctgat gtttgccgga
     6721 acagcttctt ctattaattg tgtcaaagaa tttacaccaa tagtttgtaa catggcttca
     6781 acatctgaag gacgtggccc aatgtggcgc aattgaaatt cgttttgcat gagaattgtt
     6841 tgaatgttta tcgcgcaaag atacaacagt tccaaacgtt tttcatttta acaaattgta
     6901 tgtggaaaaa tgtagaaata tgttgaaagg cacacgctta tattagtgct ttttatattt
     6961 caacttaagt aaaattgtgt ctattttttt actagctcac gttagcgtat tcctacacaa
     7021 aatatttttc tattgcagtg tcatacagtt ctttgtactc agctatcgtt ccataatttt
     7081 caccatcaac aatgatatta tttcctttga cggtgccgat taattgattt ggaatattat
     7141 tagacttcaa gtgagtttcg aaattttctt tttcatttgc acgaatagaa acaacaacac
     7201 ggctttgacc ttcgccaaat aaacaagcgt cttttcgtat cgtagtgcag gtattaattt
     7261 caaaaccgaa atttctcgtc atagcgcttt ctaaaacggc aataaacaaa ccgccttcgc
     7321 taaggtcgtg agctgattga atgagtttct tgtcaattaa actggcaaca gcatcttgta
     7381 tttttgcttc ttcttgtaag tcaaaatatg gtgcaggaga taattctact ttatggtatt
     7441 gaacaagata ttctgagcta ccaatatcat tcttactttc accaattaga taaattaagt
     7501 cgccttcttg tttgaaatct aatgtcatga tattgtccac atcttcaata ataccaagca
     7561 tgccaatcgt tggtgttgga tttactgctc tgttgggtga ttgattgtag aaggaaacat
     7621 tgccaccagt tactggtgtt tcgaactgta aacaagcatc gcccattcct tgcagtgcat
     7681 gtacgaaatt ccaataaact tcttcgtcat aaggattgcc gaaattcaag caattggtaa
     7741 tggctactgg tttcgcgcca gtgcacacaa tatttcttgc agcttcagaa acagcaattg
     7801 cgccaccttt ataggcatca gcatgtacat atctcgagtt gcaatcgaca gtaactacta
     7861 atcctttttt cgaatctttt aaacgaataa cagcagcatc gcttggtctg ttagtagata
     7921 cattgttgat gccaaccata gaatcgtatt gtttatatac aaaacgcttt gatgcaatgt
     7981 ttggatgttg caccagcatt tttgctacgt ctatatagct ttttggaaat ggtactttat
     8041 catctaaaat aaattcatct ctttttgcat ggtagctagg ttgtttgtat gaacgctcat
     8101 acaccggagc gcctccgcca agcaccaagg tgtctgcagg tacttccgct actaacgtat
     8161 catgcatgta atattgcact aaattacctg ctgtaacttc gccaataatg gcacaatgta
     8221 aatcccattt ttcaaaaact gcttcaactt ctttttcttt acctttttga actacaataa
     8281 gcattcgctc ttgcgattct gataaaagta tttcgaaagg ttgcatgttc ttttggcgag
     8341 tcggcacttt gtctaaccat attttcattc catgttcgcc ttttgcgctc atttcgttgg
     8401 tggagcaagt aatacca
//
LOCUS       DAOQQV010000112         8411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3272_length_8411_cov_9.159765, whole genome shotgun sequence.
ACCESSION   DAOQQV010000112 DAOQQV010000000
VERSION     DAOQQV010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3272_length_8411_cov_9.159765"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..376)
                     /locus_tag="PLL59_10280"
     CDS             complement(116..376)
                     /locus_tag="PLL59_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24237.1"
                     /translation="MLNFADKISILENYLLQINDNYADSFKTDIALFLGEFDNKNSNL
                     NFLNNLSSKIENWINNLISKIVLKFNEDEEQLSDFIFYALNP"
     gene            complement(370..1143)
                     /locus_tag="PLL59_10285"
     CDS             complement(370..1143)
                     /locus_tag="PLL59_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HMY24238.1"
                     /translation="MEVKGNKHENYEILNLIGYGLSKFNDDFIKEFGFKTKTAFYEYC
                     VNIGIAETVGTVKNRMDLFDHFFPNNGRKGWWQKGDAYIHRKYLIDSLFGNENVNGYS
                     NIVKLYLKENYKVKEIFVEITPIVKSRFKKLQETGLEAELYFMNNFNSINQFKNGILE
                     DARLFGDGYDFQINVNESLYLTEVKGIRANKGRFRMTENEYNKALEYKNDYLIALVLN
                     MNELPVFLTIENPANNLKFKKEERVSKPIIEYHLLSDIC"
     gene            complement(1149..1406)
                     /locus_tag="PLL59_10290"
     CDS             complement(1149..1406)
                     /locus_tag="PLL59_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24239.1"
                     /translation="MSLKQTISINEFDSKSYQIRLVEEIEEKAVLTHYLHNYRNGVKR
                     HYEKDVISTLKNFVEYKQEETELPIVAFDPKQVKDYSKLVA"
     gene            complement(1583..2623)
                     /locus_tag="PLL59_10295"
     CDS             complement(1583..2623)
                     /locus_tag="PLL59_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24240.1"
                     /translation="MEVNKKTARLLHKTIAEWQTQQVISDEEATKLKQTIVVRQFDWK
                     QVTLYAFIIAVACAVLSLIVLLADNLLQELIRKIMEISDAGISVILSVFAAFAFWFAN
                     RRKMLQPDKVIENNSFMLVATFISITAFAYWAKTWEPILHNVSIVFLLACMLYVFLSI
                     RFQSNALWILALLMLAIAFVVCSSFLENKEEKFLGMNYPMRFIPFSLLLLLAKNYLMK
                     REMFKPFLKVHYITSLLFLFIAMWMLTIFGNYTRYERWEQVNQITFLYVGILFFFFAS
                     LGMYVGLKRNDLILGNISLLFFIINLLTRYFEYFWEPLHKSVFFLVLAFLFWFIGSRA
                     EKLWNLRFLEDK"
     gene            2778..5483
                     /locus_tag="PLL59_10300"
     CDS             2778..5483
                     /locus_tag="PLL59_10300"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMY24241.1"
                     /translation="MQKTILYLFISCMTMLGYGQITLKGVVIDAKTKEPLPYLNYSVE
                     SGIGGTTDDSGRFEFLVNAKTDSVIFSYIGYKDEVLKKRFFKKDSIIVRMHLESFMLE
                     EFTVKAKRNKIPKDTVAIRLFRNVVKHKDENRPKSFDSYQFEEYQKTIASLYNISAKI
                     TQKKIFKPFRFILENQDSTADGARFIPLILKESITDHYFNKDPKKNKSVVKASKISGI
                     EQLRFSELLDIAFDEIDVYDKQAMVNEKAFVLPFADGALALYNYYLIDSVKIADTIKP
                     IDTSRFIYNNKTNYFKKAVYDTSITKQIIVDTSQLATAKSDSLQTDSIQYTYIIKIDS
                     SYVTDSFITTDTIVVRDSIWYYNLAFVPKAKGDLVFNGKATIQDSTFAITKIELGIDR
                     RSNINFINDFSLKQRFSNTGKGWFKTEESRSTNIAIMKSKKAKSVRIARYLHRDNLIV
                     NEPIADSILRQENTIFLKNYRRKTDSFWVASRHDTLSVPESKVYFLIDSLKRTRFYKA
                     MSGVGSFFASGYYKTKTLEYGNLYQMVTWNDLEGVRLRANLRNNWRIGKWVNWSIYGA
                     YGIKDKRFKYGASLSVNLPDKKDKKHSIAISFRDDYQRFTLNSNGLSYDFIYMSAFRR
                     KAIADLVYLKDAKLTYTREWFPSFTTKLEFNYKIYQTIPGKIEFIKTDAFNVKDTIKK
                     FNIFSPSINIVYTPGAKFLQGGDRVRFLKGKLPRFNFTYSFSNKKLGSHFNFHKMELI
                     VDERLPSPIGHTIFKLIGTKLFGSAPYPLLHIHAGNQSFMYNEERFTNMLETEFIADQ
                     QLTLMIEHHFDGFFFNKIPGWRKLGLREVLTTKMAVSSLDPKKVSFSDLPANMKGING
                     FYAEVGFAIENILKVVRFDFSWRLTQLNEPNIKKFRWAVSFSPSF"
     gene            5545..6201
                     /locus_tag="PLL59_10305"
     CDS             5545..6201
                     /locus_tag="PLL59_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY24242.1"
                     /translation="MDFGKLYVKYRFYAAILLIIIGILLHIFVHKGWQWSILFYITGV
                     SFILFDIFIGPMRYLQKKIEAGDIEGAKEVMSWIKFPNLLIKPVRQGYYMLQSNFDMA
                     NQDLTAAEANIRKSLANKSDMLGTSQEGVSYLQLGGIALQKGDNKEALKNLKIAVQKG
                     LPDDDNLATAYLQICTIYAQRREVRVAKEYLRRANALKVKNEEIKKHLKDINKQMARA
                     "
     gene            6376..7668
                     /locus_tag="PLL59_10310"
     CDS             6376..7668
                     /locus_tag="PLL59_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HMY24243.1"
                     /translation="MKYLPINAQLFVENRKKLVASLPPNSMAIILANDEMPRSADQAF
                     VFRQNPDLFWLSGIDQEKTILIIYPDCPNPLFREALFLRKTNEIIAVWEGHKYTMAEA
                     REASGISQIFWEESFDGALKSLMTYCDKVFVNINENDRNDNVVPYKDIRFANELKYNY
                     PAHEIKRLGPIMAKLRSVKHPLEIDTIKHACNITRDAFKRVLQFMKAGVMEYEVEAEI
                     IHEFIRQRATGHAYTPIIASGASACVLHYIENNRACNDGDLVLMDFGAEYANYAADLT
                     RTIPANGKFTQRQKDVYNAVLRVMKQAKTMLKPGVILADYNKEVGQIMEAELIGLGLL
                     DKDAVAKQDKENPLYKKYFMHGTSHFLGIDVHDIGNRYAPMQAGNVFTCEPGIYIPEE
                     KLGIRIENDILITEDGIIDLMADIPIEVEEIEDLMNTK"
     gene            8105..>8411
                     /locus_tag="PLL59_10315"
     CDS             8105..>8411
                     /locus_tag="PLL59_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24244.1"
                     /translation="MSQSSIQRLFNQYLKSAPTVAIRSKTKVHLLIDGSYFANGLCLI
                     LYYDHDIRYVQLYRETNQEKYKEIKEDLENLKKLGVDVYSVTCDGHKAILKAVRKVFP
                     "
BASE COUNT         2972 a         1394 c         1365 g         2680 t
ORIGIN      
        1 aacaaacccg aaaaatttga aactgctgaa agattttttt ggagctattt cccgctttcc
       61 gttacaatct tttttgttcg ttcttctcaa aaaaggattt ccactacaat cggggctatg
      121 gatttagtgc ataaaatata aaatcagata gctgctcttc atcttcattg aattttaaaa
      181 caatttttga aataagatta tttatccaat tttcaatttt ggaagataga ttatttaaaa
      241 agttaagatt agaattttta ttatcaaatt cacctaaaaa taaagcaata tccgttttga
      301 aactatcggc ataattatca tttatttgaa gtaaataatt ttctaaaatt gatattttat
      361 ctgcaaaatt taacatatat cagataataa atgatattca ataattggtt tagaaactct
      421 ttcttctttt ttaaatttta aattgtttgc tggattttca atagttaaaa aaactggcaa
      481 ttcattcatg tttaaaacca aagcaattaa atagtcattt ttatattcta atgctttgtt
      541 atattcattt tcagtcattc taaaacgtcc tttatttgct ctaatacctt taacttcggt
      601 taaatataaa ctttcattta cattaatttg aaaatcatac ccatctccaa acaatcttgc
      661 atcttctaaa attccatttt taaattggtt aatagaattg aaattattca taaaatacaa
      721 ttcagcttct aatcctgtct cttgtaattt tttaaatcta gattttacaa ttggagtaat
      781 ttcaacaaaa atttctttga ctttgtagtt ttctttaagg tatagtttta caatatttga
      841 ataaccgttc acattttcat ttccaaagag actatcaatc aaatattttc gatgaatgta
      901 agcatctcct ttttgccacc aaccttttct tccattattt ggaaagaaat ggtcaaacaa
      961 atccattcta ttttttaccg ttccaacggt ttctgcaata ccaatattaa cacaatattc
     1021 ataaaaagca gttttagttt taaagccaaa ttctttgata aaatcgtcat tgaattttga
     1081 caaaccatac ccaattagat taagaatttc ataattttcg tgcttatttc cttttacttc
     1141 cataaatttt aagcaactaa ttttgaataa tcttttactt gttttgggtc aaaagcaaca
     1201 attggcaatt cagtttcctc ttgtttatat tcgacgaaat tttttagagt tgagattaca
     1261 tctttttcgt aatgtctttt tactccattt ctgtaattat gcagataatg tgtcaaaacg
     1321 gctttttctt caatttcttc aacaagcctt atttgatagc ttttactatc aaattcattt
     1381 atacttattg tttgttttaa actcatttat tgtctgtcaa aaattaggtg gaaagataca
     1441 aaaaaaatag ttaattttct ttataactat tgtcctatat gcggttcggt ggcattactt
     1501 gtaactccat aatacacgct gaattcaaat atcgaacata acaataattt ttagatatcc
     1561 attaatttaa ttaaaaaacc gtttacttat cttccaaaaa tctcagattc cataattttt
     1621 cagcacggct gccgataaac caaaaaagga atgccagcac caaaaagaaa actgatttat
     1681 gtagtggttc ccaaaaatat tcgaagtagc gcgttaataa attaatgata aaaaatagca
     1741 aggaaatatt tcccaaaatt aaatcattcc gttttaatcc gacatacatt cccagtgatg
     1801 caaagaagaa aaacaaaatt cctacataaa gaaaggtaat ttggttaact tgctcccatc
     1861 gttcatatct tgtgtaatta ccaaatatcg ttagcatcca cattgctatg aaaaggaata
     1921 ataatgaagt aatataatgc actttaagaa atggtttgaa catttcccgt ttcattaaat
     1981 aattttttgc caatagcaat aataaactaa acggtataaa acgcatcgga taattcatgc
     2041 ctaagaattt ttcttcttta ttttccaaaa acgagctaca tacaacaaat gcaattgcta
     2101 gcatcaataa tgctaaaatc cacaatgcat tggattgaaa tcgaatagac aagaaaacat
     2161 ataacataca tgcgagcaag aaaacaatag atacattatg gagtattggt tcccatgttt
     2221 ttgcccaata ggcaaatgct gttatgctta taaatgtagc gacaagcatg aaactattat
     2281 tttctatcac tttatcaggt tgaagcatct ttcttctatt ggcaaaccaa aaagcaaacg
     2341 cagcaaacac tgacaatatc acgctaatgc ctgcatcgct aatttccatt attttgcgaa
     2401 tcaattcttg caataaatta tctgctagca atacgataag tgataaaacg gcacaggcaa
     2461 cagcaataat aaaagcatat aaggtaactt gtttccaatc aaactgacgt acgacaatgg
     2521 tttgttttaa tttcgtcgct tcctcatcag aaattacctg ttgtgtttgc cactctgcta
     2581 tcgtcttatg taatagacgt gctgtctttt tatttacttc cataaaaatt ttatcaatat
     2641 tactgcaaat tatcaaaaaa ccgtccaatc ataaaaaaaa tggcaaagca tttgttatga
     2701 atacattaag tagttttttt tttaacttgt acaaaaacta atctataact ttgttcgttc
     2761 ttacaaataa atattgcgtg caaaaaacta tactatacct atttatttca tgtatgacaa
     2821 tgcttggtta tggccaaatc acgctaaaag gcgtagtgat tgatgccaaa actaaagagc
     2881 cactgccgta tcttaattac tccgtagaat ctggaattgg tggcaccaca gacgacagtg
     2941 gccgatttga gtttttggtc aatgccaaaa cagactctgt catcttttcg tatataggct
     3001 ataaagatga ggttttgaaa aagcgttttt tcaaaaaaga ttctattatt gtgcgtatgc
     3061 acttggaaag ttttatgctg gaagaattta cggtaaaagc gaaacgcaat aaaattccta
     3121 aagacactgt cgctatccga ttatttagaa atgttgtaaa acacaaagac gaaaatcgac
     3181 caaaatcatt cgactcttat caatttgaag aatatcaaaa aaccatagcg tcactgtaca
     3241 acatcagtgc aaagattaca caaaagaaaa ttttcaagcc attccgtttt atattagaaa
     3301 atcaagattc tacagctgat ggtgcacgtt ttattccttt aatattaaaa gaatctatca
     3361 cagaccacta ttttaataaa gacccaaaaa agaataaaag tgttgtaaaa gccagcaaga
     3421 tatctggcat tgaacaattg cgtttttccg aattattaga tattgctttt gatgaaattg
     3481 atgtgtacga caagcaagct atggtgaacg aaaaagcgtt tgtgcttcct ttcgcagatg
     3541 gcgcattggc tttgtataat tactatttaa tcgatagtgt gaaaatagca gacacgatta
     3601 aaccgattga cacaagtaga tttatctaca acaataaaac aaactatttt aaaaaggcag
     3661 tttatgacac cagcatcacg aagcaaataa tcgttgatac tagtcaactc gcaactgcaa
     3721 agagcgactc cttacaaact gacagcattc aatatactta tatcatcaag atcgacagtt
     3781 cttatgtaac tgacagtttc attactactg acacgattgt ggttcgagat agtatatggt
     3841 attataattt agcatttgtg ccaaaagcta aaggcgattt agtatttaat ggaaaagcaa
     3901 cgattcaaga ttcgacattt gccattacta aaattgaact tggcatagac aggcgctcca
     3961 acatcaactt tatcaacgat ttttcattaa aacagcgatt tagcaatact ggaaaaggct
     4021 ggtttaaaac ggaagaatca cgcagcacca acattgctat tatgaaaagt aaaaaggcaa
     4081 aaagcgtgcg tattgctcgt tatttgcatc gtgataatct cattgtcaac gagcctattg
     4141 ccgacagtat tttaaggcaa gagaacacta ttttcttgaa aaattataga cgaaaaacag
     4201 actctttttg ggtggccagc agacacgaca cactcagcgt tccagaaagt aaagtatatt
     4261 ttttaatcga ctcattaaaa cgtacaagat tctacaaagc catgtctggt gttggcagtt
     4321 tctttgcaag tggttactac aaaaccaaga ccttagaata cggcaacttg tatcaaatgg
     4381 tgacttggaa cgacttagaa ggtgtgcgct tgcgtgctaa cttaagaaac aactggcgta
     4441 tcggcaaatg ggtcaactgg agtatttatg gtgcgtatgg cattaaagac aagcgtttca
     4501 agtatggtgc atcattaagt gtaaacttgc ctgacaaaaa agataaaaaa cacagtatcg
     4561 ctatttcttt tagagacgac tatcagcgtt ttacattaaa tagcaatgga ttgagctacg
     4621 attttattta tatgtcagca ttcagacgga aagcaatcgc agatttagtg tacttaaaag
     4681 atgcaaaatt gacatacacg cgtgaatggt ttcccagctt tactacaaaa ttagaattca
     4741 attataaaat ctatcaaacc attcctggca aaattgaatt tataaaaaca gatgctttta
     4801 atgtaaaaga caccataaaa aaattcaata ttttttcgcc aagtataaac atcgtttata
     4861 cgcctggcgc taaattctta caaggaggcg accgcgttcg tttcctaaaa ggcaaattgc
     4921 cgcgatttaa ttttacttat tccttcagca acaaaaagct aggcagtcat tttaattttc
     4981 ataaaatgga attaattgtc gatgaacgtt tgccatcgcc aattggtcat acaatattta
     5041 aactaatagg caccaaacta tttggctcgg cgccttatcc tttactacat atacacgccg
     5101 gcaaccaatc gtttatgtat aatgaagaaa gatttaccaa tatgctggaa actgaattta
     5161 ttgcagacca acaattaaca ttaatgatag agcaccattt tgatggattt ttctttaata
     5221 aaattccagg ctggcgcaaa ttaggcttgc gagaagtatt aaccaccaaa atggctgtct
     5281 caagtcttga cccaaaaaaa gtatcatttt ctgatttgcc tgccaatatg aaaggtatca
     5341 atggttttta tgctgaggtt ggatttgcga ttgaaaatat cttaaaagtt gtccgttttg
     5401 attttagttg gcgattaaca caattaaatg agccgaatat taaaaaattc agatgggcag
     5461 tcagcttctc gccaagtttt taacagctaa atcatttgca agttctttta aaaaccacta
     5521 tttttgcact tcaatttttg acacatggat ttcggaaaat tatatgttaa gtatcgcttt
     5581 tatgcagcaa tacttttaat tatcataggc attttattac atatttttgt acacaaaggt
     5641 tggcaatggt ctatcctttt ttacatcacc ggcgtttcct ttattttgtt cgatattttt
     5701 attggaccaa tgcgctactt gcaaaagaaa attgaagctg gcgacatcga aggtgcaaaa
     5761 gaagtaatga gctggataaa atttccaaat ttattaatca aaccagtacg ccaaggctat
     5821 tacatgttgc aatctaattt tgatatggcc aatcaagatt taactgcagc agaagccaat
     5881 attcgaaaaa gcttggcaaa caaaagcgac atgttaggca cttcgcaaga aggtgtttct
     5941 tatttacaac tcggtggcat tgcgctgcaa aaaggcgaca ataaagaagc cttgaaaaac
     6001 ctaaaaatag ctgtacaaaa aggcttacct gatgacgaca atcttgcaac tgcctattta
     6061 caaatctgca ctatttatgc acaacgtaga gaagtgcgcg tagctaaaga atatttgcgc
     6121 agagccaatg ctttaaaagt aaaaaacgaa gaaatcaaaa aacatctgaa agacatcaat
     6181 aaacagatgg ctagagccta attaaaaggc attttgatgg tcgagatgtt aatgcaaaaa
     6241 gcgttgcaaa atctttgaaa ttacaaaaca cacattggtg acaaaactct ttgtttttac
     6301 gaggcatcca tttgctgttg ttttgtgtat ctttacatta acaaatataa gcgttcacat
     6361 cttcaaatat aacgcatgaa gtatctacct ataaatgcac aactttttgt cgaaaatcgg
     6421 aaaaaattag tagcatcatt gccaccaaat tcaatggcaa ttatattggc aaatgatgaa
     6481 atgccacgta gtgccgatca ggcatttgtg tttcgccaaa atccagactt attttggctg
     6541 agtggcatcg accaagaaaa aacgatattg attatttatc ctgactgccc aaatccttta
     6601 tttagagagg ctttattttt aagaaaaact aatgaaatta ttgcggtttg ggaaggtcat
     6661 aaatatacta tggcggaagc acgtgaagca tctggcattt cgcaaatatt ttgggaagaa
     6721 agttttgatg gcgcattaaa atcattgatg acgtactgcg acaaagtatt tgtaaacatt
     6781 aatgaaaacg acagaaacga caatgttgta ccttataaag atatccgctt cgcgaatgaa
     6841 ttaaaataca attatcctgc gcatgaaata aaaagattag ggcccataat ggcgaagtta
     6901 cgcagcgtaa aacatccatt agaaattgac acaataaaac acgcttgtaa cataactcgc
     6961 gatgctttta aacgtgtact ccaattcatg aaagctggtg taatggaata tgaagtagaa
     7021 gcggaaatca ttcacgaatt tatacgccaa cgcgctacag gacacgccta cacaccgatt
     7081 atcgcaagtg gtgcaagtgc ctgcgtattg cattacatag aaaacaatag agcttgtaat
     7141 gacggtgatt tagtgttaat ggatttcggt gcagaatatg ccaactatgc tgctgattta
     7201 acacgcacta ttcctgccaa tggcaagttt acacaacgtc aaaaagatgt ctataatgca
     7261 gtattgcgcg tgatgaaaca agctaaaacg atgctgaaac ctggcgtgat tttagctgac
     7321 tataacaaag aagttggtca aataatggaa gctgaattaa ttggtttagg attattggac
     7381 aaagatgcgg tggctaagca agacaaagaa aatcctttgt ataaaaaata ttttatgcat
     7441 ggcacctctc attttttagg tatcgacgtg catgatatcg gtaatagata tgcgccaatg
     7501 caagctggca acgtatttac atgtgagcct ggtatttata ttcctgaaga aaaattaggt
     7561 attagaattg aaaatgatat cttaattaca gaagatggaa ttatcgattt aatggcagat
     7621 ataccaatag aagtggaaga aattgaagat ttaatgaaca ccaaataaac aaaaatgagc
     7681 tttttattta ttgcatttat gctattacta ccttttggta ttgcgatagg cttttatcta
     7741 tttaaagcgt atcaagatgc ttcaaaaaag aaataactta tttgagacaa tgtggtcaaa
     7801 aatggtgcgt aaaaatgccc ttagatgtag tgctggtagg tgttagcggt ataatatagt
     7861 gaaagtggtt tcactatatt attttaaaag atgaaaaaaa acgaataaaa agtgtcctgt
     7921 atgtcaatct aaccacatac aaaagaaagg caaaaggaat gggaatcaac gattccaatg
     7981 ttcagactgt cattataact ttattgtaaa aaatgtaaaa tctactctca ataatcaatt
     8041 tatttggttt aagaagtgga taatagaacg tcaggtttat aagtatctca ttcgagatag
     8101 tggcatgtct cagagtagta ttcaacgttt atttaatcag tatctaaaat cagcaccaac
     8161 ggtagcgata cgaagcaaaa caaaagttca tttgctgatt gatggtagct attttgcgaa
     8221 tggtttatgt ttgattttgt attatgatca tgacattcga tatgtacaat tatatcgaga
     8281 aacaaatcaa gaaaagtata aagaaattaa agaagattta gaaaatctaa agaagcttgg
     8341 agtagatgtt tatagcgtta cttgtgatgg acacaaagct attttaaaag cagtaagaaa
     8401 ggtatttcca c
//
LOCUS       DAOQQV010000113         8395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3283_length_8395_cov_6.509832, whole genome shotgun sequence.
ACCESSION   DAOQQV010000113 DAOQQV010000000
VERSION     DAOQQV010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3283_length_8395_cov_6.509832"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLL59_10320"
     CDS             complement(<1..763)
                     /locus_tag="PLL59_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY24245.1"
                     /translation="MQRDWWKWGDPNEIKHINDYPKLKTLVEDRWKTSLREEFFPPKK
                     FELPQLSDKRKEQIKDIFAVLDPKKISFKDDDRLFVSLGKSYYDVIRIFNGGGFISPD
                     VVITPTSHDEIQYILKQASDNNIAIIPFGGGTNVVGALSLDNLSGKSDIKCTLDLRKY
                     KKLIQIDEEHLTATFEAGIFGPELEKILQAKGYTLGHFPQSFEYSTLGGWIVTRGAGQ
                     ESTYYGKIEDLVEHIKVATPIGTLESNTFSHDASGI"
     gene            932..1831
                     /locus_tag="PLL59_10325"
     CDS             932..1831
                     /locus_tag="PLL59_10325"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HMY24246.1"
                     /translation="MLYHNDKSILFFNGEYKNVKDFRISPFNQTFHYGYGVFEGLRAY
                     NSHQGPHIFKVKRHFERLKNSAEKMNMQMPYSVQELINYAYELLERNNMHEAYIRPLV
                     FMDKNMTLRALDADKPNFLMTCWKWNRYYPSNHLKVLVSSWRRPHPRTMPVDIKVTGN
                     YVNSILATREAHQKGYDDALLLDVEGYIATGPGASFFMEKNGQLIVPPLTNIFPSITR
                     ATLIDIAKQMGIDVLEKQFGVEDVIEADGAFFTGTAAEVSGIESINGHKLKISWEDSI
                     GHLLHNRYKRIVSGKDTNFLEYF"
     gene            2172..3851
                     /gene="ilvD"
                     /locus_tag="PLL59_10330"
     CDS             2172..3851
                     /gene="ilvD"
                     /locus_tag="PLL59_10330"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HMY24247.1"
                     /translation="MSTLNKYSKTITQDATQPAAQAMLHAIGLSDDDFRKAQVGIAST
                     GWDGNPCNMHLNGLAASLKNLINQQDLVALNFYTIGVSDGISMGTSGMRYSLVSREVI
                     ADSIETNAGAQFYDGIISIAGCDKNMPGVIMGMSRLNRPSLMVYGGTIAPGKYKGENL
                     DVVSAFEALGKKFAGSISDEDYKGVIHHACPGAGACGGMYTANTMASAIEALGMSLPY
                     SASNPAVSEEKRNELKQAAIAIKNLLEKDIKPSDIMTFKAFENAIRIVIVLGGSTNAV
                     MHLIAMAKTIGVKLSLADFIRLGNETPMLADFRPSGKYVMEDLHKIGGVPAVMKYLLK
                     VGLLHGDCLTVTGKTLAENIAELPDLTEGQDLMHTIDNPIKPTSHIRILFGNLATEGA
                     VAKITGKEGERFEGTAKCFDNEQMAIDAMQAKKVVAGDVVVIRYIGPRGGPGMPEMLK
                     PTSVLMGEGLGKEVALITDGRFSGGSHGFVVGHVTPEAQNGGNIALVKDGDKIIIDAV
                     NNTIDVLISEEELATRKASWTAPALKAKSGILYKYAKTVSSASEGCVTDEF"
     gene            3864..3938
                     /locus_tag="PLL59_10335"
     tRNA            3864..3938
                     /locus_tag="PLL59_10335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3899..3901,aa:Val,seq:gac)
     gene            4018..4344
                     /locus_tag="PLL59_10340"
     CDS             4018..4344
                     /locus_tag="PLL59_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24248.1"
                     /translation="MSSTVNTLDFYSKWKEGAHTENSIINELRQHGFDSLQIIETLQQ
                     FKKKQLDERQTLGFIITSIGAFLGFVSCVMTMLDIFPDLRGFMLYGLTSLALVFIFIG
                     LYYIFE"
     gene            complement(4405..8364)
                     /locus_tag="PLL59_10345"
     CDS             complement(4405..8364)
                     /locus_tag="PLL59_10345"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HMY24249.1"
                     /translation="MNIHDINIDIENYRWDSTGMHIDIENLNTICDNNLHVKKIKTNA
                     LLDNDGIYLDNADIAFNDTKFKGNLSIQFTDEWRSFKYFSEKVIMKADIKEAAIKAND
                     VAVFAPKVLKYLPPQIQLKGFVKGKLANLRVENLYLTAGKNTVIDVAGNIKGLPKISQ
                     TLFDLKIKQLKSSPDDIKNMLSFVKIPKQINNAGIITFNGNFFGFTNDFVAKGELKTD
                     NLGHLVTDIRMAFPKGKAPEYSGKIIAKSLNLAEITGNKKLLGTVDIDINADGNGFKT
                     KDLNTKLSGTLRNFYFNGFVFDKIKIDGQLQKKKFTGKAFFDDDCFLVDFNGWADFND
                     SIPKYDFHTSIKNANLHQLNLTKDTLIVSLDGVIHGKGNKIDNFTGNSLFSNIILQNN
                     KDILALSVVSINMENDGTIKDYTINSDQFDARFHGVFDPLTLVPSMKVYLSKYSQLIK
                     PTAKDIKQNKPQQVDATIKLASDFGLIKVFVPNLQYISELDLKTSINTTQDKFSLLAK
                     MDSANYSNIAFSGINIEGNTNNNNLFLNGTINNTSIKKTNLNNIDIGVNSSKEQLLTN
                     LKIANDTAQNAVQLLSSLDFKNDSIIARIIDSKLKVNNKIWTIEKGNELIFVDSIFIA
                     QNFSLVEGKQRININNGKNSLSDAKINIENLNLSDVGQLIDSTGAIKNGSLSGTINLK
                     NILTKLQANADITIHDLQILNYKVKYIGLDGIYGRNGKKIVEAGGTIEDGDYQLSFDG
                     TYDMQLKGKEQLDVNADIEKLNLNFLEALLKKELLVPRAFVKGQVNVSGSLKKPILTG
                     KARIIDTAELKMRLLGTTFKLVDEEILLTNKGFDFGTVTLFDNYGNTALLSGKLLHNG
                     FKEWRADKVNVSAPSGYNFMNTTYEDNQDFYGKVFAKGDVDIDGYFNNININVNRLET
                     LKNTVFNLPVSNKVSDKGYSFVNFVNPNDTVKKIVYKSKISGLNMMMNISATQDAEVN
                     IILDQSANDKISGRGYGDLNFTMDKKGEMSLNGVYNLTDGKYDFNFQGILNKTFKIGP
                     GSKITFNGDPLKAELAVNALYNIKSASVRNLFDSTNTEAKSIMNRTFPIDLNLLISGT
                     LDKTAIGFKIQPSGVVPDVLDRKLVEINGNQNEVNNQAGFLLLFNSFFPTGVSEQKIA
                     GYSNTVTQLISDQISRLLSQGLSSVIKGASLDVLLTDLETESRNFGFSYKQEILGGKL
                     ILTIGGNVNFGNNNSVSPSNPTGQTSNNAAIAGDFQLEYLVTADGRIRLKTYARTANY
                     DLINQDRVRTGVAISFQKDFDNFKDLFSRKARKQKPTIDTSNMHQQINFVPLKDSIK"
BASE COUNT         2711 a         1342 c         1401 g         2941 t
ORIGIN      
        1 ttattccgga agcatcatgt gaaaaagtgt tgctttctaa tgttcctata ggtgtggcta
       61 ctttaatatg ctctactaaa tcttcaattt taccataata tgtggattct tgaccagcgc
      121 ctcttgtcac tatccaaccg cctaaagtgg aatactcaaa agattgaggg aaatgaccta
      181 aagtatagcc ttttgcttgc aatatttttt ctaattctgg gccaaaaatg ccagcttcaa
      241 aagttgccgt taaatgttct tcatcaatct gaattagctt cttatattta cgtaagtcga
      301 gcgtacattt aatgtctgat tttcctgata aattatctaa agaaagcgca cctacaacat
      361 tagtaccacc tccaaatgga ataatggcta tattattatc agaagcttgt tttagaatat
      421 attgaatttc atcgtgtgat gttggtgtaa tgacaacatc tggtgaaata aaaccaccac
      481 cattaaaaat acggataacg tcgtaatagc ttttgcctaa tgatacaaat aaacggtcat
      541 cgtctttgaa cgaaattttc tttggatcta aaacagcaaa tatatctttt atttgttcct
      601 ttcttttgtc tgataattga ggaagttcaa atttctttgg cggaaaaaat tcttctctca
      661 agctggtttt ccatctgtct tctactaatg ttttgagctt tggataatca ttaatatgtt
      721 ttatctcatt tgggtcaccc catttccacc agtctctttg catgtcaaaa tattttgatg
      781 taatttacag tataacagca taatatttgc ataaaataat tattaactct acattctatt
      841 ttatttttag gaaaggcaaa aatcaatatt ataaaatcgt aattttagta tattagatac
      901 actttctgta gtattttatt taattttcag tatgttatac cacaacgaca aatcaattct
      961 tttttttaat ggcgaataca aaaatgtaaa agactttaga ataagtcctt tcaatcaaac
     1021 atttcattat ggctacggtg tttttgaagg attaagagcc tataattccc atcaaggacc
     1081 acatattttc aaagtcaaac gccactttga acgtttaaaa aattcagcag aaaaaatgaa
     1141 tatgcaaatg ccatattctg ttcaagagtt gattaattac gcttatgaat tattggaaag
     1201 aaataatatg cacgaagcct atatcaggcc tttagtattt atggataaaa atatgacctt
     1261 acgtgcttta gacgctgaca aacccaactt tttaatgacc tgttggaaat ggaatcgtta
     1321 ttatccttct aatcacttaa aagtattggt ttcttcttgg cgtaggccac atccaaggac
     1381 aatgcctgta gatattaagg ttacaggtaa ttatgtcaat tctattttgg ctactcgtga
     1441 agcacaccaa aaaggatatg atgatgcttt attattagat gttgaaggtt atattgctac
     1501 tggaccagga gcatcatttt ttatggaaaa aaatggtcaa ttaattgttc caccactaac
     1561 caatattttt ccaagcatta ctcgtgctac attaatagat atagccaagc aaatgggaat
     1621 tgatgtttta gaaaaacagt ttggcgtgga agacgttatc gaggctgatg gtgcattttt
     1681 tactggtacg gcagcagagg tttcaggtat tgaaagcatt aatgggcata aattaaaaat
     1741 ttcttgggaa gactcaatcg gtcatctatt gcataaccga tataaaagaa tagtttctgg
     1801 aaaagacact aattttttag agtatttcta atgatgattt aagtgtcatg taatttttag
     1861 ccaataattt tacgcgatac tgttaatgta tctaagcatt tttattagtt ttagaactaa
     1921 taaattttta ctttgcaaaa gtaacaatta taaataacgc tatcaaaaca acagagaaga
     1981 tagcaacaaa atacttgtgc aaatcaattt caactcgttt atcggacatg cttttttatg
     2041 tttacataaa tataacatat accaaaaaca tactatttat agagttttgc acatatctgc
     2101 attattaaca acaaaagatt aaactaaaaa ttgctcaatc ttttgttatt ttgaaaatct
     2161 aaataaaaaa catgtcaact ttaaataaat acagcaaaac aatcacacaa gatgctacac
     2221 agccagcagc acaagcgatg ttgcacgcta tcggtttaag tgatgatgat tttagaaaag
     2281 cacaggtagg cattgcatca actggctggg acggcaatcc ttgtaacatg cacttaaatg
     2341 gcttggctgc gtctttaaaa aatcttatta atcaacaaga tttagtggca ttaaattttt
     2401 atacaattgg cgttagcgac ggcatctcaa tgggaacatc tggtatgcga tattcattag
     2461 tatcacgcga agtgattgct gattccattg aaacaaatgc tggcgctcaa ttttatgatg
     2521 gtattatttc aatagcaggt tgcgataaaa atatgcctgg cgtcatcatg ggtatgtcgc
     2581 gcttgaacag accatcatta atggtatatg gtggcactat tgcacccgga aaatataaag
     2641 gagaaaatct agatgtcgtt tctgcatttg aagcattagg taaaaagttt gctggttcaa
     2701 ttagcgatga agattacaaa ggtgtaatac atcatgcatg tccaggagca ggtgcttgtg
     2761 gtggaatgta cacagcaaac acgatggcat ctgccattga ggcattaggc atgagtttgc
     2821 catattctgc aagcaatccc gcagtttcag aggaaaaaag aaatgaatta aaacaagcag
     2881 cgattgcaat aaaaaactta ttagagaaag acattaaacc aagcgatatc atgacgttca
     2941 aagcatttga gaatgctatt cgcatcgtta tcgttttagg tggtagcaca aatgcagtta
     3001 tgcacttgat agcaatggcg aaaacgatag gtgtgaaatt gtctttagct gactttattc
     3061 gattaggcaa tgagacacca atgcttgccg attttagacc aagtggcaaa tacgtaatgg
     3121 aggatttgca taaaattggt ggtgtacctg ctgtaatgaa atatttatta aaagtaggct
     3181 tattacatgg cgattgtctt actgtaacag gaaaaacatt agctgaaaat attgcagaac
     3241 tgccagattt aacggaagga caagacttaa tgcacacaat tgacaatcca ataaaaccta
     3301 caagtcatat taggatttta tttggaaact tggcaacaga aggcgcagtt gcaaaaatta
     3361 caggcaaaga aggcgagcgt tttgaaggaa cagcaaagtg ttttgacaat gaacaaatgg
     3421 caatagatgc tatgcaggca aagaaagtag tggcaggaga tgtggttgtt attcgatata
     3481 ttgggccacg tggtggacca ggcatgcctg aaatgttaaa gccaacttct gttttaatgg
     3541 gcgaaggatt aggcaaagaa gtggctttaa taacagatgg cagatttagt ggtggctctc
     3601 atggttttgt agttggccac gtaacacctg aggcgcaaaa tggtggaaat attgcattgg
     3661 taaaagatgg cgataaaatc attattgatg cggtaaataa tacaatagat gtattgattt
     3721 ctgaagaaga attagctacg agaaaagcat cgtggacagc acctgctttg aaggctaaat
     3781 ctggaatatt atataaatat gcaaagacag tatcatctgc cagcgaaggc tgcgtgacag
     3841 atgagttcta gtttgtatat attgggcatt tagttcagct ggtttagagc actacctcga
     3901 caaggtagaa gtcattggtt cgaatccaat aatgcccact tttttagtaa aaagctatta
     3961 tttgttagaa ttttagttta attttaaata cctaaatggt taatttaata ttaatctatg
     4021 agttcaacag taaatacttt agatttttat tcaaaatgga aagaaggtgc acacactgaa
     4081 aattccatta ttaatgaatt gcgtcaacat ggttttgata gcttacaaat cattgaaaca
     4141 ttacaacaat ttaagaaaaa acaattagat gaacgccaaa cattgggttt cattattaca
     4201 agtattggtg catttttagg atttgtaagt tgtgtaatga caatgttgga tatttttcct
     4261 gacttacgtg gttttatgtt atatggttta acaagcttag ctttagtttt tatctttatt
     4321 ggattgtatt atatattcga ataataaact gcatttctaa tttcatttat taactaataa
     4381 gtaatgtagt aagttctaca ttaattattt aatggaatct tttaaaggta caaaattgat
     4441 ttgttgatgc atatttgatg tgtcaatcgt tggcttttgc tttctggctt tgcgagaaaa
     4501 taaatctttg aaattatcaa aatctttttg gaaagaaata gcaacgcctg tacgcactct
     4561 gtcttggttg attaaatcat agtttgctgt acgcgcataa gttttcaagc gaattctacc
     4621 atcagcagtc accaaatatt ctaattggaa atcgcctgca atagcggcat tattgcttgt
     4681 ttgtcctgtt ggatttgaag gagaaaccga gttgttattt ccaaaattaa cattaccacc
     4741 aatagttaaa ataagctttc cgcctaatat ttcttgctta tagctgaatc cgaaattgcg
     4801 tgattctgtt tctaaatctg ttaataaaac atctaatgaa gcgcctttaa tgacactgct
     4861 taaaccttgt gaaagcaagc gcgatatttg atctgaaatt aattgtgtaa cggtattgga
     4921 atagcctgca attttttgct ccgaaacgcc agtagggaaa aatgaattaa acaataataa
     4981 aaaaccagct tggttattga cttcattttg attgccattt atttctacta attttctgtc
     5041 taaaacatct ggaacgacac ctgaaggctg aattttaaaa cctattgccg ttttgtctaa
     5101 agtgcctgaa attaaaagat tcaagtctat tggaaacgtt ctattcatga tagattttgc
     5161 ttccgtgttg gtagagtcaa acaaattacg aacagatgct gatttgatat tgtataaagc
     5221 atttacggca agctcagctt ttaaaggatc gccgttaaat gtaattttgc tgccaggacc
     5281 aattttaaat gttttgttta atattccttg aaaattaaaa tcgtatttgc cgtctgtaag
     5341 gttatatacg ccatttaatg acatttctcc ttttttatcc atggtaaaat tcaaatcacc
     5401 gtaaccacga ccacttattt tgtcattagc actttgatcg agaattatat tgacttctgc
     5461 atcttgtgtt gcagaaatat tcatcatcat atttaaacca ctaattttag atttatatac
     5521 aatcttcttt accgtgtcat ttggatttac aaaattgaca aacgagtatc ctttatcaga
     5581 cactttgtta gaaaccggca aattgaaaac tgtgtttttt aatgtttcca atcgattgac
     5641 attgatattg atattattaa aatagccatc aatatctaca tcaccttttg caaatacttt
     5701 accataaaag tcttgattgt cttcataggt tgtattcata aagttatagc cacttggcgc
     5761 actaacattt actttgtcgg cacgccattc tttaaatcca ttatgcaata attttccaga
     5821 caataaagct gtatttccat agttgtcgaa taaagtaaca gtaccaaaat caaatccttt
     5881 attcgttagt aaaatctctt catcaacgag cttgaaggtt gtgccgagca atcgcatttt
     5941 taattctgca gtatcaatta tccttgcttt acctgttaaa ataggctttt tcaagctgcc
     6001 agaaacattg acttgtcctt taacaaaagc acgaggcacc aataattctt ttttcaataa
     6061 tgcttctaag aaatttaaat ttaatttttc tatatcggca ttgacatcca attgttcttt
     6121 gcctttcagc tgcatatcat acgtgccatc aaaagaaagt tgataatctc catcttcaat
     6181 tgtgccacct gcttctacta ttttttttcc atttctacca tagattccat ctaaacctat
     6241 gtatttaact ttataattga gtatttgtaa atcatgaatt gttatatcag catttgcttg
     6301 tagtttagta agaatattct tcaagtttat tgtgccactt aatgatccgt tttttatagc
     6361 gccagtggaa tcaattaatt gcccaacatc tgaaaggttt aaattttcaa tattgatttt
     6421 tgcatcagaa agcgaatttt ttccattgtt aatgtttatt cgttgtttgc cttcgaccaa
     6481 tgaaaagttt tgagcaatga atatactgtc cacaaaaata agttcattgc ctttttcaat
     6541 tgtccaaatt ttattattta cttttaattt agaatcgata atacgtgcaa taatactatc
     6601 gtttttaaaa tctaagcttg acaaaagttg aacggcgttt tgtgcggtgt cattagcaat
     6661 tttaagatta gtcagtaatt gttctttcga gctgttaaca cctatatcta tgttgtttaa
     6721 attggttttc ttaatgctag tattgttgat ggttccatta agaaataaat tattattatt
     6781 ggtattacct tcaatattaa taccactaaa tgcaatatta ctatagtttg cagagtccat
     6841 ttttgccaac aatgaaaact tatcttgtgt agtattaatg ctagttttta agtcgagttc
     6901 gctaatgtat tgtaagttcg gaacaaatac ttttatcaac ccaaagtcag aagcaagctt
     6961 tatagttgca tctacttgtt gtggtttgtt ttgtttaata tcttttgctg ttggttttat
     7021 taattgtgaa tatttgctta aatatacttt catggaaggc accaatgtaa gcggatcaaa
     7081 aacaccatga aagcgcgcat cgaactggtc agaattgatt gtgtaatctt taattgtacc
     7141 atcattttcc atgtttatag atacaacaga aagtgcgaga atgtctttgt tgttttgtaa
     7201 aataatatta ctgaaaaggc tatttcctgt gaagttgtct attttatttc ctttgccatg
     7261 aattacgcca tctaaggaaa caattagtgt gtccttcgtt aggtttagct gatgaagatt
     7321 tgcatttttt atgcttgtat gaaaatcgta ttttggaata gaatcattaa aatctgccca
     7381 accattaaaa tctacgagga agcaatcatc atcaaaaaat gcttttcctg taaatttctt
     7441 tttttgcaat tggccgtcaa ttttaatttt gtcaaacaca aaaccattaa aataaaaatt
     7501 ccgtaacgtg cccgacagtt tggtatttaa gtcttttgtt ttaaaaccat ttccatcagc
     7561 atttatatca atatctacag tgcctaagag ttttttattg ccagtaattt cagccaaatt
     7621 taaacttttt gcaattattt ttcctgaata ttctggtgct tttcctttag gaaatgccat
     7681 cctaatgtcg gttactaaat gacctaaatt atctgttttc aattcacctt tagcgacaaa
     7741 atcatttgta aatccaaaga aattgccatt aaaagtaata atacctgcat tatttatttg
     7801 ttttggaatt ttcacaaatg acagcatatt tttaatatca tcaggtgatg attttaattg
     7861 ttttattttt aaatcgaata aagtttgact tatcttcggc aatcctttta tgtttccagc
     7921 aacatcaatt acggtatttt ttccagcagt taaataaaga ttttcgactc tcaaattcgc
     7981 caacttacct tttacaaatc cttttaattg aatttgtggt ggcaagtatt ttagcacttt
     8041 tggcgcaaac acggcaacgt catttgcttt gatagctgct tcttttatgt cggctttcat
     8101 gattactttc tcagaaaaat atttaaaaga acgccactca tctgtaaatt gtatagatag
     8161 atttcctttg aactttgtgt cattaaatgc aatatctgca ttgtctaaat aaataccatc
     8221 attatcaagc agtgcatttg tttttatttt ttttacatgt aaattattgt cacaaatagt
     8281 atttagattt tcaatatcga tatgcatacc tgtagaatcc caacgatagt tttctatgtc
     8341 aatattgata tcgtgaatat tcatttggct aatcagaaaa gtaccagcag cttga
//
LOCUS       DAOQQV010000114         8375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3298_length_8375_cov_6.980048, whole genome shotgun sequence.
ACCESSION   DAOQQV010000114 DAOQQV010000000
VERSION     DAOQQV010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3298_length_8375_cov_6.980048"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLL59_10350"
     CDS             complement(<1..233)
                     /locus_tag="PLL59_10350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HMY24250.1"
                     /translation="MLVFVIYKIILVLVVFAISLGVAAYSTYAERKVAAFMQDRVGPD
                     RAGPFGLLQPLADGLKMFMKEEIIPTVSNKFLF"
     gene            complement(233..1264)
                     /locus_tag="PLL59_10355"
     CDS             complement(233..1264)
                     /locus_tag="PLL59_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMY24251.1"
                     /translation="MAKVTIDDITIVVPDGTTILHAARMIDEQHGTNIAPPTMCYYSK
                     LKATGGYCRTCIVNVTQGSEKDPRPMPKPVASCRTTVMDGMVVKNNTSPELLEARAGV
                     VEFLLLNHPLDCPVCDQAGECHLQDLSYENGKEGSRFEFPKREIEPDDLGDKIKLNMN
                     RCILCYRCVKVCDQITDGRVHGVLYRGDHAEISTYIEKAIDNDFSGNVIDVCPVGALT
                     DKTFRFKSRVWFTKPVDAHRNCAHEKCSGKVRLWFKGDDVLRVTARKDRWGEVEEFIC
                     NECRFDKKSKHDWEIEGPSIISHHSVINQNHYTLGKLQLGIKNQIKHLNGTKIDLGEG
                     ALNPNNIEK"
     gene            complement(1264..2607)
                     /gene="nuoF"
                     /locus_tag="PLL59_10360"
     CDS             complement(1264..2607)
                     /gene="nuoF"
                     /locus_tag="PLL59_10360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HMY24252.1"
                     /translation="MNEKLLLKYDHIPGINTLAVYRQHNGYKSLEKALKTLKPDEVVE
                     EVKKSGLRGRGGAGFPTGMKWSFLAKPEGVPRHLVCNADESEPGTFKDRYLMERIPHL
                     LVEGMIIASYALGANASYIYVRGEYYYIIKILQKAIKEAYAAGLLGKNILGSGFDLDL
                     YVQPGGGAYICGEETALLESLEGKRGNPRIKPPFPAVAGLWGRPTVVNNVETIAAVVP
                     IIQDGGDAYAKIGVGKSTGTKLISASGHINKPGVYEIELGVPVEEFIYSEQYCGGIRN
                     GKKLKAVVAGGSSVPILPAELILKTAKGEPRLMTYESLSDGGFATGTMLGSGGFIVMD
                     EDTSIVKNLFTFARFYRHESCGQCSPCREGTAWMEKIIKKFIDGKANMADIDLLWDVQ
                     SKIEGKTICPLGEAAAWPVAAAIRHFRHEFEAYVRNPQSINEVKHYYRLNNLVEA"
     gene            complement(2647..3165)
                     /locus_tag="PLL59_10365"
     CDS             complement(2647..3165)
                     /locus_tag="PLL59_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HMY24253.1"
                     /translation="MTTSEIKFNDEAQKKVQEILAQYPNDKSKSALLPLLHLAQAEFD
                     GWLSPETMDYIASILNIQAIEVYEVATFYSMFNLKPVANYLIEICQTSSCWMCGAEDI
                     VRYLEKKLNVAVGEPTQDGMFQIKTVECLGSCGTAPMFQIGDKYYENLTFEKIDAILE
                     DLRKENKRSRYV"
     gene            complement(3223..4416)
                     /gene="nuoD"
                     /locus_tag="PLL59_10370"
     CDS             complement(3223..4416)
                     /gene="nuoD"
                     /locus_tag="PLL59_10370"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HMY24254.1"
                     /translation="MTNTYSTYDEKQYSTLNLGPTHPATHGIFQNVLTMDGEIIIDAV
                     STVGYIHRAFEKIAERRPYNQITTLTDRLNYCSSPINNMGWHMTVEKLIGAEIPKRAQ
                     YMRVIIMELSRIADHIVCDTVIAVDTGAMTGFLHLFQSREWIYEIFEEICGARLTTNI
                     GRIGGLERDFSPKVWDKINSFITEFPKKLVEFQKLIDRNRIFMDRTINVGGISVDMAL
                     DYGFTGPNLRAAGLDYDVRVANPYSAYDEFEFDIPVGTNGDTYDRYMVRMEEMWQSLR
                     IIQQAVKNIPDGSFHADLPEFYLPEKQEVYSKMEALIYHFKIVMGETEVPKGEVYHSV
                     EGGNGELGFYLISDGGRQPFRLHFRRPCFIYYQAYPEMIRGGMLSDAILTMSSMNVIA
                     GELDA"
     gene            complement(4461..4967)
                     /locus_tag="PLL59_10375"
     CDS             complement(4461..4967)
                     /locus_tag="PLL59_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMY24255.1"
                     /translation="MSELLEIVKNKVQAKFGDALLASEMHYDFPVFIISKEKIKDVLQ
                     FLKEDKELHFHFLTTLCGLHHPEQDSSIEFGVMYQLHNLPKNWRIRLKTFTSREDLNI
                     PTVTDLWKTAGWMERQEFDFFGINFIGHPNLSRILNMDEMNYHPMRKEYPLEDMSRDD
                     KNDKMFGR"
     gene            complement(5021..5575)
                     /locus_tag="PLL59_10380"
     CDS             complement(5021..5575)
                     /locus_tag="PLL59_10380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HMY24256.1"
                     /translation="MSTTAIDINPAYQPEGLEGQGFFATSLDKVVGLARANSLWPLPF
                     ATSCCGIEFMATMAAHYDLARFGSERLSFSPRQADLLMVMGTISKKMGPILRQVYEQM
                     AEPKWVLSMGACASSGGIFDSYSVLQGIDRIIPVDVYIPGCPPRPEQVLDGILQIQKL
                     AESEQRRRRESPEYKALLAQYGIE"
     gene            complement(5600..5974)
                     /locus_tag="PLL59_10385"
     CDS             complement(5600..5974)
                     /locus_tag="PLL59_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HMY24257.1"
                     /translation="MILSANQISVFLPVVLMFLVALGFVAFVMVVTHWMGPKRNSKTK
                     LETFECGIDVQGNARSPFSIKYFLVAILFVLFDVEVIFMYPWAVNFKKLGILGIIEMF
                     TFISVFLVGFFYIIKKGALKWE"
     gene            6181..8358
                     /locus_tag="PLL59_10390"
     CDS             6181..8358
                     /locus_tag="PLL59_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMY24258.1"
                     /translation="MHISELPDEKQQLATEYVLRTDVNVFVTGKAGTGKTTFLRAIQQ
                     NNTKKTVVAAPTGVAAINAGGMTLHSLFQLPFLPFVPTKQRVPATFVNMHTLFESFKF
                     SKAKIELIRELELLVIDEVSMLRCDMLDCIDVILRTIRKKQNEVFGGVQVLFIGDLFQ
                     LPPVVQDEEWNFLQDYYNTPFFFDAQVMKEAHFVNIEFDKIYRQNEERFIRLLNKVRN
                     NDMSEDDFHGINERYQPELIADLDNYITLTTHNYKADKINQGQLQKLEGDLTSFIAEV
                     NGEFNEKSYPTEYTLQLKTGAQIMFIKNDSQPEKKYYNGKLGIVKKISNDGILIQFIE
                     NEEEYLLGKETWENIRYSYSNNSDKIEEEKIGSFTQYPIRLAWAITIHKSQGLTFEKA
                     VIDAGQSFAAGQVYVALSRCSTLQGMYLLTPISAQAIKSDERIINFTENYLTNESTLL
                     EQLPFHKIEFAKKELIKAFDWSKVVDTVMQLAIVVEEKKLPEKEKALNLFNEMKNHAH
                     TQLQTANKFSAQLQNILVHIEEAENQALLHERVVKSILYFGQHLADNIIKPLQDYCHD
                     IQVKKGVKGFLKIADNAEQTLITKLKQIERLQFGENLFYNGESFLKTESKLLQEISKK
                     KTIKGESSMESLRMYKDGKNIQEIANARALTTSTIEGHLANFVATGELNILDFLKEQD
                     IHDIRLAIDTINTTQLSPLKTHLNDRFTFGQLKMAIAKIGTIK"
BASE COUNT         2637 a         1588 c         1390 g         2760 t
ORIGIN      
        1 ataaataaga atttattcga tactgttgga ataatttctt ctttcataaa cattttcaaa
       61 ccatctgcca atggttgcaa taatccgaaa gggcctgctc tatctgggcc taccctatct
      121 tgcataaatg cagctacttt tctttctgca taagtagaat atgcagcaac accaagtgag
      181 atggcaaata ccaccaacac taatatgatt ttatatatta caaataccag cattattttt
      241 ctatattatt aggatttagt gcaccttcgc ctaaatctat ttttgtacca tttagatgtt
      301 taatctgatt tttaattcct agttgcaatt tccctaaagt ataatgattt tggttgatga
      361 cagaatgatg cgaaattata cttggtcctt cgatttccca atcgtgtttc gattttttat
      421 caaagcgaca ttcattgcaa ataaactctt ctacttcgcc ccacctatct tttctggctg
      481 taacgcgtaa tacatcatca cctttaaacc ataaacgaac ttttcctgaa catttttcgt
      541 gtgcacaatt gcgatgtgca tcgactggtt tagtaaacca aacacgactt ttaaatcgga
      601 atgttttatc tgtcaatgcg ccgactggac aaacatcgat tacgtttcca gaaaaatcat
      661 tatcgattgc tttttctata tatgttgaaa tttcggcatg gtcgccacga tatagcacac
      721 catggacacg gccatctgta atttgatcac aaacttttac acatctataa cacaagatgc
      781 atctgttcat gtttagtttt attttatcgc ctaaatcatc tggttctatt tcgcgtttag
      841 gaaattcaaa acgagagcct tcttttccat tttcataact taaatcttgt aaatgacatt
      901 cccctgcttg gtcgcaaaca ggacaatcta gtggatgatt aagcaacaga aattcgacca
      961 cacctgcccg tgcttctaat aattctggcg aagtattatt ttttactacc atgccgtcca
     1021 ttactgtcgt tctgcagctt gctactggtt ttggcatcgg tcttggatct ttttcagatc
     1081 cttgcgttac attcacaata catgttctac aatagccacc tgttgctttc agtttagaat
     1141 aataacacat ggttggcggt gcaatatttg taccgtgttg ttcgtctatc attcttgcag
     1201 catgcaagat agttgtgcca tcaggcacta ctatggtgat atcgtctata gtaacttttg
     1261 ccattatgct tctactaagt tatttaatcg ataatagtgt ttcacttcat ttatagattg
     1321 tggattgcgc acgtacgctt cgaattcgtg tctaaaatgt cggattgcgg ctgccacagg
     1381 ccaagctgct gcttcgccga gtggacaaat tgtttttcct tcgattttgc tttgcacgtc
     1441 ccatagcagg tcgatgtctg ccatgtttgc ttttccgtct ataaattttt taattatttt
     1501 ttccatccaa gcggtgcctt ctcgacaagg agagcactgt ccacagcttt catgtctgta
     1561 gaaacgtgcg aatgtaaata aatttttcac gattgaagta tcttcatcca taacgatgaa
     1621 gccaccagaa cctaacatcg tgcctgttgc gaatccacca tcactcaagg attcataggt
     1681 catcaggcga ggttcacctt ttgctgtttt cagaattagc tcagctggca agataggaac
     1741 ggaagagcca cccgccacaa ctgcttttaa tttttttcca tttctgatac caccacagta
     1801 ttgttcacta taaataaatt cttcgacagg aacacctaat tcgatttcat aaacaccagg
     1861 tttattgata tgtccagatg cggagatgag ttttgttcct gttgattttc ctactccaat
     1921 ttttgcatat gcatcgcctc catcttggat aataggtaca actgcggcaa ttgtttccac
     1981 attatttact acagttggac ggccccaaag gccagctacg gctgggaatg gcggtttaat
     2041 tcttggattt cctcttttac cttctaatga ttcaagcaaa gctgtttctt cgccacagat
     2101 gtaagcacca ccaccgggtt gaacatataa atctaaatcg aagcctgagc ctaaaatatt
     2161 tttacccaac aagccagcgg cataagcttc ttttatcgct ttttgtaaaa ttttgatgat
     2221 atagtagtac tctcctctca catagatata tgatgcattt gcaccgagtg cataacttgc
     2281 gataatcata ccctctacca ataagtgtgg tatgcgttcc attaaatacc tgtctttgaa
     2341 agtgcctggc tctgactcgt cggcattgca aactaagtga cgcggcacac cttcaggttt
     2401 tgccaagaaa ctccatttca ttcctgtagg gaaacctgca ccaccacgac cgcgtaaacc
     2461 agatttttta acttcttcta ctacttcgtc tggctttaat gttttcaatg ctttttctag
     2521 tgatttatag ccattgtgtt gacgataaac cgctaaagta ttaataccag gaatatgatc
     2581 gtattttaat aataacttct cgttcatttg tataaagttt tcacttcgtt tagataggca
     2641 atcaaattat acgtatctgc ttcttttatt ttcttttctt aaatcttcta atattgcgtc
     2701 aatcttttcg aatgttagat tttcatagta tttatcacca atttgaaaca taggagctgt
     2761 accacatgaa cctaagcact ctacagtttt tatttggaac attccatctt gtgttggttc
     2821 gcctactgcg acatttagtt ttttttcgag ataacgtaca atatcttcag caccacacat
     2881 ccaacaagag cttgtttgac aaatttcaat taaataattt gcgacaggtt ttaaattaaa
     2941 catagaatag aaagtggcta cttcatatac ttcaattgct tgaatattta aaattgaagc
     3001 aatataatcc attgtttcag ggctcaacca accatcaaat tctgcttgtg ctaaatgcaa
     3061 taatggcaac aatgccgatt tgcttttatc atttggatat tgagctaaaa tttcttgcac
     3121 ttttttttgt gcttcatcat taaattttat ttcgcttgtc gtcatctatt ttcttttaat
     3181 tcatcatttt catttttttt aaaaaaatga ttttaaatat tactaagcat ctaactcgcc
     3241 tgcaataaca ttcatgctac tcatggttaa gatagcatcg ctaagcattc cacctcgaat
     3301 catctctgga tatgcttgat aataaataaa gcatggtctt ctgaaatgca aacggaaagg
     3361 ctgacggcca ccgtcactaa ttaggtaaaa acctagttca ccatttccac cttcaacgct
     3421 atgatatact tctcctttag gcacttctgt ttcgcccatc acgattttaa agtgataaat
     3481 aagcgcttcc attttgctat atacctcttg tttttctggt aaataaaact caggcaaatc
     3541 agcatgaaag ctgccgtctg gaatgttttt tacagcttgt tgaataatgc gtaagctttg
     3601 ccacatttct tccatacgca ccatatatct atcgtatgta tcgccgtttg tgccaacagg
     3661 aatatcgaat tcaaattcgt cataagcaga atatggattg gcaacacgca catcataatc
     3721 taaacctgca gcacgcaaat ttggacctgt aaaaccatag tctaatgcca tatccacact
     3781 aattccacca acatttatgg ttctatccat aaaaattcgg tttctatcaa tcagcttttg
     3841 aaactctact agctttttag gaaattctgt aataaaacta ttgattttgt cccatacttt
     3901 aggagagaaa tctcgttcta agcctcctat tctaccgatg tttgtggtca agcgtgcgcc
     3961 acatatttcc tcaaaaattt cgtatatcca ttctctactt tggaatagat gcaagaagcc
     4021 tgtcatcgca cctgtatcta ctgcaattac tgtatcacat acgatatggt cagcgatgcg
     4081 agataattcc ataataatca cacgcatata ttgtgcgcgt ttaggaattt ctgcacctat
     4141 caatttttca actgtcatgt gccaacccat attatttata ggtgaggaac aatagtttaa
     4201 tctatctgta agtgtggtaa tttgattgta aggacgacgc tctgcaattt tttcaaaagc
     4261 tctatgaatg taaccaactg ttgacactgc atcaatgatg atttcaccat ccatggttag
     4321 tacattttgg aagataccat gtgttgctgg atgtgtgggt ccaaggttta aagtggaata
     4381 ttgtttttcg tcgtatgtag aatatgtatt tgtcattttt tacaagttta gtttgcacta
     4441 tttattaaat agcacaaata tcatctacca aacattttat cgtttttatc atctctcgac
     4501 atatcttcta aaggatactc tttacgcatt ggatgatagt tcatttcgtc catatttaaa
     4561 attctcgata gatttgggtg accaataaaa ttgattccaa agaaatcaaa ctcttgtctt
     4621 tccatccaac ctgccgtttt ccataaatca gttacagtag gaatattcaa atcttctctt
     4681 gaagtaaatg ttttaaggcg aattctccag tttttaggta aattatgtag ttgatacatt
     4741 acaccaaact cgatactgct atcttgttct gggtgatgaa ggccacataa tgttgtaaga
     4801 aaatgaaaat ggagttcttt atcttctttt aagaattgta agacgtcttt aattttttct
     4861 ttgctgatga taaaaacagg aaagtcgtaa tgcatttctg atgctaataa agcatcgcca
     4921 aactttgctt gtactttatt ttttactatt tctaacaatt cactcatgct ttctattttc
     4981 ggaaattttt atgtcaatat actgacctat ttttccatga ttactctata ccatactgcg
     5041 ccagcagtgc tttatactct ggtgactctc tacgtcttct ttgttcgctc tcggctaatt
     5101 tttgaatttg taaaattccg tcaagcactt gttctggacg aggtggacaa cctggaatat
     5161 atacatctac cggaattatt ctatcaatac cttgtaagac gctataagaa tcaaaaattc
     5221 cgccgctgct tgcacaagcg cccatcgaca agacccattt tggttcggcc atttgctcat
     5281 atacctgacg taagatgggt cccatttttt tagagatggt gcccattacc attaataaat
     5341 cagcttgacg tggtgagaag ctcaaacgtt cagaaccgaa gcgtgctaaa tcgtaatgtg
     5401 ctgccatagt agccataaat tcgatgccac agcaagaggt tgcgaatggc aatggccaca
     5461 atgaatttgc cctagctaag cctactactt tatctaatga agtcgcaaaa aaaccttgcc
     5521 cttccaagcc ttctggttgg taagccggat taatatctat tgctgttgtg ctcatttttt
     5581 ttgttttaca atctgggatt tattcccatt ttaatgctcc tttcttaatt atatagaaga
     5641 agcctactaa aaatacagag ataaatgtaa acatttctat aatacctaaa atgcctaatt
     5701 tcttgaagtt aactgcccaa ggatacataa atatcacttc tacgtcgaat aagacgaata
     5761 aaatggctac taagaaatat tttatagaaa agggcgaacg tgcatttcct tgaacgtcga
     5821 taccgcattc aaaggtttct aatttcgttt tagaatttcg ttttggaccc atccaatgtg
     5881 taactaccat tacgaaagct acaaaaccaa gtgctactaa aaacatcagc accaccggca
     5941 aaaaaactga aatctgattt gcagaaagta tcataatatt tacaacatgc aaagatacta
     6001 ttatgtttta ataattcaga tgtgattttt atcacatttt aataaatgtg gaataaattt
     6061 aaacaatgac tatcaatggt ttataaaaag aactcctttt tattttgatt tatatatgga
     6121 aatcagggag atattgtctc tcaatagtat attaaatagt aaatcattat ttttgaatcg
     6181 atgcatattt ctgaattacc tgatgagaag caacaattag ccactgagta tgtattgcgt
     6241 actgatgtca atgtatttgt aacaggaaag gctggcactg gaaaaacgac atttctacgt
     6301 gctattcaac aaaacaatac taaaaaaact gtagttgcgg cgcctactgg tgttgccgca
     6361 ataaatgcag gtggcatgac cttgcactct ctttttcagt taccatttct gccatttgtg
     6421 ccaacaaagc aacgtgttcc agcaacattt gtgaacatgc atactttatt tgagagcttt
     6481 aaatttagca aagctaaaat agaattaatc agagaacttg aattacttgt catagatgag
     6541 gtaagtatgt tgcgttgtga tatgctagat tgtattgatg taatattacg tactataaga
     6601 aaaaaacaaa acgaggtatt tggtggcgtt caagtattat ttattggcga tttatttcaa
     6661 ttgccacccg tggtgcaaga tgaagaatgg aatttcttac aagattatta taacacacca
     6721 ttcttctttg atgctcaagt gatgaaagaa gcacattttg taaatattga attcgacaaa
     6781 atatatagac agaatgaaga acgatttatc cgattactta ataaagtaag gaataatgat
     6841 atgagtgaag atgattttca cggcataaac gaacgttacc aacctgagct aattgctgat
     6901 ttagacaact acattacatt aactacgcac aattataaag ctgataaaat caaccaagga
     6961 caacttcaaa aattagaagg cgatttaaca agttttatcg cagaagtcaa tggtgagttt
     7021 aacgaaaaat cttatcctac tgaatatact ttacagttga aaactggtgc gcaaattatg
     7081 tttattaaaa atgatagcca gccagaaaag aaatactata atgggaaatt aggcattgta
     7141 aaaaagataa gcaatgatgg cattctcatt caatttattg aaaatgagga agaatattta
     7201 ttgggcaaag aaacgtggga aaacattcga tattcataca gcaacaatag tgacaagata
     7261 gaagaagaaa agataggcag ttttacacag tatccaattc gtttggcatg ggcaataacc
     7321 attcataaaa gccaaggtct gacttttgag aaagcagtta ttgatgctgg ccaatcattt
     7381 gcggccgggc aagtatatgt agcattgagt agatgctcca cgctgcaagg tatgtatttg
     7441 cttacaccta ttagtgcgca agcaataaaa agtgacgaaa gaattatcaa ttttaccgaa
     7501 aattatctaa ctaatgaaag cacattatta gagcaattac cgtttcataa aatcgaattt
     7561 gcaaagaaag agttaataaa agcgtttgac tggtcgaaag ttgtagatac tgtaatgcag
     7621 ttagcaattg tagttgaaga aaaaaaattg cctgaaaaag aaaaagcatt gaatttgttc
     7681 aatgaaatga aaaatcatgc acacacacag ttgcaaacag ccaataaatt ttctgctcaa
     7741 ttgcaaaata tacttgtgca catcgaagaa gcagaaaacc aagctttatt acatgaaaga
     7801 gtagtaaaat caattttata ttttggacag catttagcag acaatatcat caaacccttg
     7861 caagattatt gccatgacat acaagtaaaa aaaggtgtga aaggattttt aaaaattgct
     7921 gataatgcgg agcaaacctt aattactaag ttaaaacaaa tagaacgatt gcaatttggc
     7981 gaaaatttat tttacaatgg tgaatcattc ttaaaaactg aaagcaaact gctacaagaa
     8041 atatcaaaaa agaaaactat taaaggtgaa tctagcatgg aaagtttgcg catgtataaa
     8101 gatggaaaaa acattcagga aattgcaaac gctagagcct tgacaacatc aacgattgaa
     8161 ggtcatttgg caaatttcgt tgctactggc gagctaaata ttttagattt cctaaaagaa
     8221 caagatatac atgacattag attggctatt gataccatta acacaactca gctttctcca
     8281 ttaaaaacac atttaaatga tcgatttact tttggccagt taaaaatggc tatcgcaaaa
     8341 attggaacga taaaatgaat tagattgcat ttatt
//
LOCUS       DAOQQV010000115         8336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3324_length_8336_cov_9.260476, whole genome shotgun sequence.
ACCESSION   DAOQQV010000115 DAOQQV010000000
VERSION     DAOQQV010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3324_length_8336_cov_9.260476"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..695
                     /locus_tag="PLL59_10395"
     CDS             255..695
                     /locus_tag="PLL59_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMY24259.1"
                     /translation="MLSKSQARTFFLGGTVVTFLAFIGLTVYSLQPNNDQTNHDKIDA
                     KVVRGKELWESNNCMGCHTILGEGGYYAPELTKVIDRRGENYVKAVLQSPVPWAPRGR
                     KMVVYKMNDKDTEALIAYLKWIGNINLNGFDQVVSPLAKDKIKN"
     gene            718..2064
                     /locus_tag="PLL59_10400"
     CDS             718..2064
                     /locus_tag="PLL59_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMY24260.1"
                     /translation="MKYKSQKVAYWFFALCMLLFSLQIVYGFIMGFARIGMDGLHDFI
                     PFNTARAVHTNLLVVWLLTGFMGAAYYIIPEESQRELVNVKLAYVQLISLAVVGVVAI
                     VGYHFNYWEGRKFLEIPRELDFLVVINVLLFLGLILATLFTGKRKTTTALVLSMGLLF
                     AALLYLPGMIWFDSQVTDSFFRWWVVHLWVEGVWELIMGGILSYLLIKLTGVDREVIE
                     KWLYVIVGLTFLSGILGTGHHYYYIGVNKIWLIVGGIFSALEPLAFLAMALFAMYMYR
                     KGEKSHPNKIALFWTIGASIVSFIGAGLLGFAHTLPQTNIYTHGTLVTAMHGHYAFWG
                     AYAMIVLAIISYVMPNVTGRKLYNNARGHFAFWCANIGMLGMTVAFGVAGVAQVYMER
                     KMKMEFMEVQNEISIHFVVLLLCATLFATGIILYIIEFLGYGKPTDEALVAAESID"
     gene            2147..2926
                     /locus_tag="PLL59_10405"
     CDS             2147..2926
                     /locus_tag="PLL59_10405"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HMY24261.1"
                     /translation="MNTNPIFYKALGAEIDVFKQADALKLPLLLKGPTGTGKSRFVEY
                     MSHEVGKKLITISCHEETSATDLIGRFIIKGAETVWIDGPLSSAVKNGHILYLDEVAE
                     ARPDVIVVIHALTDHRRELYIDKLGESIKAHPDFLLVASFNPGYQKGFKELKPSTRQR
                     FVALSFDYPTPKIEANILVNETGIDENTATKLVAIATKIRNLTELGLTETVSTRLLVD
                     AAKLIHNGLPKRLAVHVAIVEPLTDELETVQALKDLCDLMI"
     gene            2933..4696
                     /locus_tag="PLL59_10410"
     CDS             2933..4696
                     /locus_tag="PLL59_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMY24262.1"
                     /translation="MGLELDEIIYSKVLKFIKKRKRNNADLLSRKADLETLKPRLIIM
                     ARAICGSPIELFPAEREGAYRDDNFFLPAQCALFPTKALNTQFYIFRTVYLSVQKQLN
                     LNFHNASDVQDTATQKAMETAPLVLEKLFKDFPALEQWYFDFIPHLPLKKDESIDYSM
                     LYGKWMCNTQQQQADTLQHIDEQAKTLQNNIKAKTTLNVKAVESIESLTIDKKQQEDY
                     VLLHSFEKIETAEEFNGNWRDFDGKDDLKDHQEALEEMNMRFTVRVDDTVHSVYQADF
                     IENTTVSESAETDEKGFHLKYDEWDFNARKYKNNYCKVYPKTQLKTNTAYYKQTLTTY
                     NTLLLGLRKMLVNINNRLQQQRKQLYGESFDLDALTDVYTDIHSGVSPDERTYLSKRK
                     KEKDISILLLLDISLSSDSYAAGNRILDVEKMVSILFGEILNEYNIEFAIDGFYSKTR
                     NNTSYLTLKEFSDKWSIAKHRIGAIEPSGYTRIGPALRHAGARMKAKSSAKNKWIIIL
                     SDGKPNDYDKYEGQYGIHDIKQALKELNQQGIHSYALAIETQAKYYLPQMFGQNHYQI
                     LQSPAALLQAMAKLFEKIKHL"
     gene            4696..5271
                     /locus_tag="PLL59_10415"
     CDS             4696..5271
                     /locus_tag="PLL59_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMY24263.1"
                     /translation="MTNTTTKSIAYPPGGILIWIIIYLELITFGMAMVALAYYGMQER
                     ALFHHDSQLLNKEIATINTIFLITGGFFAAKAVHCFKAKNIDRSARMLLWAMLAGVGF
                     LLLKMFEYYGKLEAGLSMDYSSFYMNYWLLTGFHWIHVFVGVIILFFIRRSIVHKKEL
                     ATIEDIEAGVAFWHMCDLIWLLLFPILYLLF"
     gene            complement(5532..5993)
                     /locus_tag="PLL59_10420"
     CDS             complement(5532..5993)
                     /locus_tag="PLL59_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HMY24264.1"
                     /translation="MELYLKNVRGKGRGVFCTEDLHDGDIIEVCPVIICPPKDCKHLD
                     KTNLYHYYFQWGNDGKSAGVVCGYGPMYNHSYNPNAVYETFYEKAIFRVKARRFIPAN
                     TEITINYNFYPDEQTPVWFDVEKQKQEKKKAKTMQHMASKTSLKKKTTKAK"
     gene            complement(6054..7823)
                     /gene="argS"
                     /locus_tag="PLL59_10425"
     CDS             complement(6054..7823)
                     /gene="argS"
                     /locus_tag="PLL59_10425"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMY24265.1"
                     /translation="MNIFEQIQTQVQQAFPALFGTAVEIEKITINETLKEFSGDVTLV
                     VFPLLKLTKKNPEESARIIGEFLLSNNPHVESFNVIKGFLNLVLKDAFWTAFLLEEPM
                     QIPAKNEVVLIEYSSPNTNKPIHLGHVRNNVLGYALAGIFKANGYQTSKVNLINDRGI
                     HICKSMIAWQKFANGETPESSGMKGDKLVGKYYVAFDKQYKQEIETLIATGHTKEEAE
                     KQAPILLAAQAMLLRWENGDAEVVDLWKMMNAWVYAGFDVTYKNLGVDFDKYYYESNT
                     YLLGKDIVEDGLSRGVFYKKENGSVWIDLSADGLDEKLVLRADGTSVYITQDLGTADL
                     KYQDFKMNRSIYVVGNEQDYHFKVLKLILQKLGRPYADGIQHFSYGMVDLPSGKMKSR
                     EGTVVDADDLIDDMIAAAKEQTEALGKIDDFTEEEKIALFKTIGLGALKFFLLRVDPK
                     KRILFDPKESIDLHGYTGPFVQYTYARTRSILRKAGSITPLAKSDLAAYALLASEKEL
                     IKILYKSNIILAECCKEMNPAKLIDYTYDVAKIYNKFYNDCAILAAEKEIEKKCRLQL
                     TTLTGNTIQRCFALVGIDVPERM"
     gene            8059..>8336
                     /locus_tag="PLL59_10430"
     CDS             8059..>8336
                     /locus_tag="PLL59_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HMY24266.1"
                     /translation="MRDRYIDLIEQTYYFPQDGFDIEDNWLRFHKIDLKHVIEQYGAP
                     LKITYLPKIGDNINRARRWFQEAFESVGYNGHYYYCYCTKSNHFSHVL"
BASE COUNT         2661 a         1408 c         1474 g         2793 t
ORIGIN      
        1 aaattaatta aacaagtacc taatccaaaa ccagtacaat cttggacttg ggtttgtata
       61 aaatttgatc cacatgttag agtgaagtaa aacctgaata ttgcagtatc ttttattata
      121 gtattagctg atttcaaaca tacgtgctta tgattattat catgtttatt tgaataattg
      181 taatctaatt ttgacctaag aaaagtacta gaccacatgt ctattcatct atcaatcaaa
      241 ctaaattttt aattatgtta tctaaatcac aagcaagaac attcttttta ggtggcaccg
      301 ttgttacatt tttagccttt attgggctta cagtgtattc attgcaacct aataatgatc
      361 agaccaatca cgacaagata gacgctaaag tcgtgcgtgg caaagaactc tgggaatcta
      421 ataactgtat gggttgccac accatcttag gtgaaggcgg ctattatgcg ccagagctga
      481 ctaaagtcat cgaccgtcgt ggcgaaaatt acgtgaaagc agtgctgcaa tcaccagttc
      541 catgggcgcc tcgtggcaga aaaatggtag tgtataaaat gaatgataaa gacacagaag
      601 cattgattgc ttatttaaag tggataggaa atatcaactt aaatggcttt gatcaagtgg
      661 tttctccatt agcaaaagac aagattaaaa attagtaaca aaaataaatc gaatattatg
      721 aaatacaaat cacagaaagt agcatactgg tttttcgcct tgtgtatgct cttattttcg
      781 cttcaaatag tatatgggtt tattatgggt tttgctcgta taggcatgga tggtcttcat
      841 gattttattc cattcaatac ggcaagagca gtacacacca atttacttgt agtttggctt
      901 ttaacaggat ttatgggcgc agcatattac atcattcctg aagaatcaca acgcgaactc
      961 gtcaatgtca agcttgctta tgttcaatta atttcattgg cagtagtggg tgtggttgcc
     1021 atcgtcggct atcactttaa ttattgggaa ggtcgtaagt ttttggaaat tcctcgagag
     1081 ctcgattttc tcgttgttat caacgtttta ttattcttag gtttaatatt agctacctta
     1141 tttacaggaa aacgcaaaac aacgacagcc ttagttttat caatgggttt attgtttgcc
     1201 gcgctattat atcttccagg aatgatatgg tttgatagtc aagtaaccga ctctttcttc
     1261 cgctggtggg ttgttcactt atgggttgaa ggtgtttggg aattaatcat gggcggtatt
     1321 ttatcttact tactaattaa attgacaggt gtagatagag aagtcataga gaagtggtta
     1381 tacgtaattg ttggtttaac tttcttatct ggtattttgg gaacaggtca ccactactac
     1441 tatattggtg tcaataaaat ttggttaatt gttggcggta tcttctctgc actagaacca
     1501 ttagcattct tagcaatggc attattcgct atgtatatgt atcgcaaagg tgaaaaatca
     1561 catccaaata aaattgcact attctggact atcggtgcat ctatagtatc attcataggc
     1621 gctggtttat taggtttcgc tcatacatta ccacaaacaa acatttacac acacggaaca
     1681 ttagttacag ctatgcatgg tcactatgca ttttggggcg cttatgccat gattgtttta
     1741 gcaatcatta gctatgtgat gccaaatgtt acaggcagaa aattatataa taatgctcgc
     1801 ggtcatttcg cattctggtg tgcaaacatt ggtatgttag gcatgacggt tgcatttggt
     1861 gtagccggcg ttgcacaagt ctatatggaa cgaaaaatga aaatggaatt catggaagta
     1921 caaaatgaaa tcagtattca cttcgttgta ttattgcttt gtgctacact ttttgctaca
     1981 ggtattatat tgtatataat agaattttta ggatatggca agccaactga tgaagcatta
     2041 gttgctgccg aaagtattga ttgatatttg tctattttgg tgcctataat agtattacgc
     2101 tttatgcatt actgttatag gtacttttat ttttaaaaaa attatgatga acacgaatcc
     2161 cattttttat aaagcactag gtgcagaaat tgatgtgttt aagcaggctg atgctttgaa
     2221 attaccgcta ttacttaaag ggccaactgg caccggaaag tcgcggtttg tagaatatat
     2281 gtctcatgaa gtaggtaaaa aattaattac aataagctgt catgaagaga catctgcaac
     2341 agatttaatc ggaagattta ttattaaagg agctgaaaca gtctggattg atggtccatt
     2401 gtcaagcgcc gtaaaaaatg gacatatttt atatcttgac gaagtagcag aagcacgtcc
     2461 agatgttatt gttgtcatac acgcattaac agaccacaga cgagaattat atatcgacaa
     2521 attaggtgag tctataaaag cacacccaga ttttttatta gttgcttctt ttaatccagg
     2581 ttatcaaaaa ggatttaaag aattaaaacc ttcaacacga caacgttttg ttgccttgtc
     2641 ttttgattat cctactccaa aaatagaagc aaatattttg gtaaatgaaa caggaatcga
     2701 tgaaaatact gcaactaagc ttgttgctat tgccacaaaa attaggaatc taacagaatt
     2761 aggattaaca gaaactgttt cgacaagatt gttagtagat gctgcaaaat taatacataa
     2821 cggcttgcca aaacgcctcg cagtgcacgt tgccatagta gagccactta cggacgaact
     2881 cgaaacggtg caagctttaa aagatttgtg tgacttaatg atatagcaaa acatgggttt
     2941 agaattagat gaaatcattt attccaaagt ccttaagttt atcaaaaaac gcaaaaggaa
     3001 caatgctgat ttgctcagcc gcaaggctga tttagaaaca cttaaaccca gattaatcat
     3061 catggcacgt gctatttgcg gcagtcctat cgaattgttt ccggcagaac gagaaggtgc
     3121 ttaccgcgat gataattttt tcttgcctgc acaatgtgct ttgtttccga ctaaagcatt
     3181 gaatacacaa ttctatattt ttagaacagt ctatttatct gtccaaaaac aattgaattt
     3241 aaacttccat aatgcatctg acgttcaaga tacagcaaca caaaaagcaa tggaaacagc
     3301 gcctcttgtg ttagaaaaat tatttaaaga ttttccagct ttagagcaat ggtattttga
     3361 ttttattcca catctgccat taaaaaaaga cgaaagcatt gactactcaa tgctgtatgg
     3421 aaaatggatg tgtaacacac aacaacaaca agcggatact ttacagcata tcgatgaaca
     3481 agcaaaaaca ctgcaaaata atatcaaagc aaaaactacg cttaatgtaa aagccgttga
     3541 atctattgag tctttgacta tcgataaaaa gcaacaagaa gattatgtgt tgttgcatag
     3601 ctttgaaaaa attgaaacag cagaagagtt taatggcaat tggcgcgact tcgatggtaa
     3661 agatgatttg aaagatcatc aagaggcact cgaagaaatg aatatgcgct tcactgtgcg
     3721 cgttgatgac acagtacact ccgtgtatca agctgatttt atagaaaata caacagtttc
     3781 agaaagtgca gaaacggatg aaaaaggatt tcatcttaaa tacgatgaat gggattttaa
     3841 tgcaagaaaa tataaaaata attattgtaa agtatatcca aaaacacaat taaaaacaaa
     3901 tacagcatac tataaacaaa ccctaacaac ttataacaca ttgctattgg gtttgcgtaa
     3961 aatgttggtc aatatcaata atcgattgca acaacaacgc aaacaattat atggtgaatc
     4021 ttttgactta gatgcattga cagatgtata tacggatatt cattctggtg tgtctccaga
     4081 cgaacgtact tatttatcta aacgaaaaaa agaaaaagac atttctattt tactgctctt
     4141 agatattagc ttgtcgagcg atagttatgc cgcaggaaac agaatattag atgtcgaaaa
     4201 aatggtgtcg attttgtttg gtgaaatatt gaatgaatac aatatcgaat ttgcgattga
     4261 cggcttttat tcaaaaacaa gaaacaatac ctcttatcta actttaaaag aattttcgga
     4321 taaatggtca attgcaaaac atagaatagg tgctatagaa ccaagtggct atacacgcat
     4381 aggaccagct ttgcgacatg ctggcgcacg tatgaaggca aagtcaagtg ctaaaaataa
     4441 atggattatt attttatcag atggcaagcc taatgactac gataaatacg aaggtcaata
     4501 tggtattcac gatatcaaac aagcattaaa agaattaaat caacaaggaa ttcattctta
     4561 tgcattagct atcgaaacgc aagcaaagta ttatttgccg caaatgtttg gacaaaacca
     4621 ttatcaaata ttacaatcgc cagcagcatt attgcaggca atggcaaaat tatttgaaaa
     4681 aattaaacac ttataatgac gaatacaact actaaatcta ttgcttatcc accaggtgga
     4741 atattgattt ggattatcat ttatcttgag ctgattacct ttggaatggc aatggtggca
     4801 ctggcttatt atggcatgca ggaacgtgca ctctttcatc acgatagcca attactcaat
     4861 aaagaaatag caacgataaa caccattttc ttaattactg gtggcttttt tgccgctaaa
     4921 gcagtgcatt gttttaaagc taaaaacatt gatagatcag cacgaatgct gttgtgggca
     4981 atgcttgctg gtgtcggatt tttgttgcta aaaatgttcg aatattacgg aaaattagaa
     5041 gcaggcttaa gcatggacta tagctcattt tatatgaatt attggttact cacaggtttt
     5101 cattggatac acgtttttgt cggcgtaatt attctgtttt tcatacgcag atcaatagta
     5161 cataaaaagg aacttgcaac tattgaagac atagaggcag gtgtagcgtt ttggcacatg
     5221 tgcgatttga tttggctttt attatttcca atcctttatt tactctttta aacgtaaaca
     5281 tgaaattgac ttctacgtat attgttttgt ttgtttgcac tatatttatt gcactaattt
     5341 caggacttgc tttgtcaaag cctattatag ctactattgt agttgtttct gtaattaaat
     5401 ttctattggt cggttttcag tttatggaac taaaaatagc acacaatttt tggaaatata
     5461 tttttgtcat ctacagtgta gtgataggtg caattttctt tttattgttg taagctaaga
     5521 aagcgaatta tttattttgc ctttgtcgtt ttctttttca atgatgtttt gcttgccatg
     5581 tgttgcatgg tttttgcttt tttcttttct tgcttttgct tttctacatc aaaccataca
     5641 ggcgtttgct catcaggata gaaattataa ttgatagtaa tttctgtatt tgcaggaatg
     5701 aaacgtcttg cttttactct gaaaatcgcc ttttcataaa atgtttcata cactgcattt
     5761 ggattataag agtgattgta cattgggcca tatccacaaa caacaccagc gcttttgcca
     5821 tcatttcccc attgaaagta gtaatgatat aagtttgttt tatctaaatg cttgcagtct
     5881 ttaggcggac aaataattac aggacatact tcaatgatat caccgtcatg taagtcttct
     5941 gtgcagaaga cacctctgcc tttgcctcta acatttttta aatataattc cattgctgtt
     6001 tttttggatt ttatttttga taagcgattt atttataata atagaattgt aatctacatg
     6061 cgttctggaa catcaatacc gactaaggca aagcatcgtt ggatagtatt gccagtgagt
     6121 gtggtaagct gtagtctgca tttcttttct atttcttttt cggctgctaa tatagcacaa
     6181 tcattgtaga atttattata gatttttgct acatcatatg tgtagtctat caactttgct
     6241 ggattcattt ctttgcaaca ttcagctaag atgatatttg atttgtataa aatttttatc
     6301 aactcttttt ctgatgcaag taatgcatag gcagccaaat cacttttagc taagggcgtt
     6361 atgctgccag ccttgcgcaa tatgcttcgc gtgcgtgcgt atgtatattg tacaaatggt
     6421 ccagtgtatc catgtaagtc aatactttct ttagggtcaa ataaaattct ttttttagga
     6481 tcaacgcgca gtaaaaagaa tttcaaagca cctaatccta ttgttttaaa aagtgcaatt
     6541 ttttcctctt ccgtaaagtc atctattttt cctaaagctt ctgtttgttc ttttgctgcc
     6601 gcaatcatat cgtcaattaa atcatcagca tctacaaccg tgccttccct tgatttcatt
     6661 ttgccacttg gtaaatctac cataccatac gaaaaatgtt gaatgccatc agcatagggt
     6721 cttcctaatt tttgaagtat taatttaagt actttgaaat gataatcctg ctcattgccg
     6781 acgacatata ttgagcgatt cattttaaaa tcttgatatt ttaagtcagc agtgcctaag
     6841 tcttgtgtaa tataaacaga agtgccatca gcacgtaata ccaatttttc atctaagcca
     6901 tcagcactca aatctatcca aacacttcca ttttcttttt tataaaaaac gccacgcgat
     6961 aagccatctt ctacgatatc ttttcctaat agataggtgt tgctttcgta ataatattta
     7021 tcaaaatcta cacctaggtt tttataggtt acatcgaagc cagcatacac ccaagcgttc
     7081 atcattttcc ataaatctac cacttcggca tcgccatttt cccaacgtaa caacatggct
     7141 tgcgctgcta ataaaatagg tgcttgtttc tctgcctctt cttttgtatg gccagtagct
     7201 attaatgttt ctatttcttg tttatattgt ttgtcaaacg ctacataata ttttccaacc
     7261 aatttatcac ctttcatacc tgagctttca ggcgtttcgc cattggcaaa tttttgccaa
     7321 gctatcatac tcttgcagat gtgtatgcca cgatcattaa tcaaatttac tttcgatgtt
     7381 tgataaccat tcgccttgaa aattccagct aatgcatagc ctaatacatt gttacgaaca
     7441 tgtcctaagt gaatgggttt attggtgttc ggcgatgagt attcgatgag tacaacttcg
     7501 ttttttgcag gaatttgcat cggttcttcc aaaagaaatg ctgtccaaaa tgcatctttc
     7561 aatacaagat ttaaaaagcc tttgattaca ttaaatgact caacatgtgg attgttagac
     7621 aataaaaact caccaattat tctagcggat tcttccggat tttttttagt gagtttcagt
     7681 agtggaaaaa caaccaatgt tacatcacca ctaaactctt ttaaagtttc attgatagtg
     7741 attttttcta tttctaccgc tgttccaaat aatgctggaa atgcttgttg tacttgtgtt
     7801 tgtatttgtt caaatatatt catagcgacc taacaaagat attgcttttg aaacaagcaa
     7861 ttagattaaa ttatcaaaaa atttaattga tagcactacc acatatttga acttaatagt
     7921 acaaatgaag tataaatgag catcacacgt ggtgtatagt aggctactgc taaatttttt
     7981 gtataaaata ccatcaaaga tattattttt gcagagcata tattttgtga tttttgaata
     8041 ttaaagctat acaaacgaat gagagaccgt tatattgacc ttattgaaca gacatattat
     8101 tttccacaag atggatttga tatcgaagac aattggctta gatttcataa aatagatcta
     8161 aagcatgtca ttgagcaata tggcgctcct ctgaaaatca cctatttgcc taaaattggc
     8221 gataatatca atcgcgctcg tcgatggttt caggaagcat ttgaaagtgt tggttacaat
     8281 ggtcactatt actattgcta ttgcacaaag agtaatcact tttcacatgt attaga
//
LOCUS       DAOQQV010000116         8290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3361_length_8290_cov_7.108440, whole genome shotgun sequence.
ACCESSION   DAOQQV010000116 DAOQQV010000000
VERSION     DAOQQV010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3361_length_8290_cov_7.108440"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..976
                     /locus_tag="PLL59_10435"
     CDS             221..976
                     /locus_tag="PLL59_10435"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HMY24267.1"
                     /translation="MKLLISAISLLLTLHVQAQFTSTNGSNEKYIEVKTTDTLMVNPD
                     AISLLIALGEKKEKNYYFDDDQTDAEKEEEARKEKNDAIQKKKQIEEILKKNNVSAFK
                     FHDKKEDKDLFTKEFNLYENTYEVELKTDAQVEKIKKEMIVLKDVSTKVANSKLNDKE
                     KYELILIDKVMKKAEREASAIAKTMNVTLDKALNVSNQSVNDIYSSMFNSPESMGGMG
                     ALFSMMGNMFKGATQQNTQVVVSKTLIVRFAIK"
     gene            981..1382
                     /locus_tag="PLL59_10440"
     CDS             981..1382
                     /locus_tag="PLL59_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMY24268.1"
                     /translation="MFNKFSTDYGLLIIRIGIGVMFALHGFPKITGGPEKWEMIGKAM
                     ENIGIGFAPTFWGFMAAVAEFVGGICLVLGFLFRPATAMLLFTMFIAAFMHYVNGDGF
                     SGYSHAVESAIVFLGLFFSGPGKYALKISRR"
     gene            complement(1392..2471)
                     /locus_tag="PLL59_10445"
     CDS             complement(1392..2471)
                     /locus_tag="PLL59_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24269.1"
                     /translation="MSDSFSENNKNISNELQCKDCGALLLFAPGTEKLACKYCGAQNE
                     ILADVENQEIEEMDYHDFIANKIKQEDKQTVNTVNCNNCGATTTLKPNITSDNCAFCA
                     SPLVIKGGTTSTILKPKYILPFKVDDKTAHDIFIKWLHGLWFAPNDLKKYASVSEKLK
                     GVYMPYWTYDSNTNTSYDGERGEYYYEGSGNNKQRKTKWYPVQGTTQHAFDDVCVSAS
                     QSLPDNTMRTLEPWDLEALVAYKEAYISGFQTECYQTDVIKGYKTAKYLMEEAIKDKI
                     RREIGGDEQRIHNMHVAYSDIRFKHILLPIWISAYSYHSKVFQFIINGRTGEVQGARP
                     YSAIKIALAIIAVISLFVLMKYMGN"
     gene            complement(2553..>3161)
                     /locus_tag="PLL59_10450"
     CDS             complement(2553..>3161)
                     /locus_tag="PLL59_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HMY24270.1"
                     /translation="ANQLRALVVTKLTDAIAESKLKIEEFASNLEEFSKFGAEKLSDD
                     FDAYGLKVTSLLVENISMPDEVKKEIFELSRLDKIDMQKLTQWKTAKGIEKAAENGGG
                     LAGAFVGVGLGGIMQGGIAGSQQGGGAVPPPVMQIFVAVNGAQTGPFDVPALTQMAQS
                     GQLKADTLVWKAGMAAWAAASTLPELAAVLNSVPPPLAPPPL"
     assembly_gap    3162..3261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3262..3494)
                     /locus_tag="PLL59_10455"
     CDS             complement(<3262..3494)
                     /locus_tag="PLL59_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMY24271.1"
                     /translation="MGILDTFRNEFIDIIEWTDNSNDTIVWKFPRFQNEIKTGAQLTV
                     RESQVAVFLNEGKLADVYQPGRYELTTANMPILT"
     gene            3669..4745
                     /gene="gcvT"
                     /locus_tag="PLL59_10460"
     CDS             3669..4745
                     /gene="gcvT"
                     /locus_tag="PLL59_10460"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HMY24272.1"
                     /translation="MKTTALTDTHVALGAKMVDFAGFYMPVRYTSDLDEHHCVRNGVG
                     MFDVSHMGEFILKGEQALPLLQKIVSNDISKLEKGQAMYGYLPNEQGGIVDDLLVYAL
                     EDGNYMCVVNAGNIDKDWNWFNQHNTENAELHNISENVALLAIQGPKAAAALQSLTDI
                     DLSSIPYYRFKRGVFAGVENVIVSNTGYTGAGGFELYFHKSNAVAVWNKIIEAGKPYG
                     LQPIGLGARDTLRLEKGFCLYGNDIDDSTSPLEAGLGWVTKFNKDFIAKEILQQQKTN
                     GLSKKLVGFTMVDRGIPRGHYIIKDAHGNPIGQVTSGTQSPTLGIGIGLGYVNIEYST
                     IDAEIYIDIRGKLLKAKVVKLPFL"
     gene            4767..5555
                     /locus_tag="PLL59_10465"
     CDS             4767..5555
                     /locus_tag="PLL59_10465"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HMY24273.1"
                     /translation="MIKNAFKISIVFTVFILFLFYIAISTAWFGISTGVGGDFCEAAR
                     NGLIKQPANSYSNIGFLISGLACAWILSHKDIDKSGAFFKHPFIPLFYCLITILLGPC
                     SMAMHATETALGGLFDMNSMYLFGSFMFAFALARYYKLHSFVFVILYFSSVFLCNYAG
                     TFKTVFGYNFFPGSAAFGVVCVLGMLFEILNYKKNKPDIQFKYAIYCSLSFLVGFGVW
                     QFGYDGHCFCNPNSLFQWHGVWHLLCALSTYFLFQFYISEKTPE"
     gene            5557..6783
                     /locus_tag="PLL59_10470"
     CDS             5557..6783
                     /locus_tag="PLL59_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HMY24274.1"
                     /translation="MKWLVCIIIGVFGIIACKENNINTSAPDAYLGNTYISHRYKDRL
                     SAVQLIDRLSAVYPNADSLIRAQYDVTVYRIFYKTHNFQNQEISASGLVYIPEIRHYF
                     APVVCFQHGTCIQKQEVPSIAANLSYYVPFIIASETGAIVCAADYIGLGFSDGKHHFY
                     EPTEEANAVVDMLGSVQPLLNKTYQKITFDDSVLLFGYSQGGHATLAAQRLLETKYDA
                     HFHIQASAPMASWFSFENSSQLNVLKAALPFDFPSAYAYLINSIQSTQSVFSSYNEML
                     QSPYDSLCSVLFDGTKSTGYVNSKFPATFHAALQTSFVNELNSNPDNNFITALKKYDI
                     INDWIPKSSTRFYHSQGDEIAFYDNSEIAYNTFKNKGGNVHLINLGNYNHFEANIIAI
                     DSVRNWFYPLIKIKPY"
     gene            7199..7639
                     /locus_tag="PLL59_10475"
     CDS             7199..7639
                     /locus_tag="PLL59_10475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMY24275.1"
                     /translation="MSSVMAQSPIAIYDLTQYENRILQKNDTLYVVNFWATWCKPCVT
                     ELPFFESNAQHFSEFPVKIILVSQDAKTRSAQVSDFLKKNNYTSEAFILSAGNPNIWI
                     DKIDNRWTGAIPMTLFYKNGNKEYFHEGEYSTAEELESYIHTKL"
     gene            7643..8251
                     /locus_tag="PLL59_10480"
     CDS             7643..8251
                     /locus_tag="PLL59_10480"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMY24276.1"
                     /translation="MQKVLITLFAIISICFTTQAHHGYHVGDNIADFSLKNIDGKNVS
                     LANYTKAKGFIIVFTCNHCPYAKAYEDRIIALDKKYAALGYPVIAINPNDAVQYPEDD
                     YASMQQRAKEKGFTFPYLHDETQAVTKTFGAIKTPHIFIVKKENTNLNLKYVGTIDDN
                     WEDASAVTETFVVNAVDELLADKPVSVANTKAVGCSIKWKKP"
BASE COUNT         2529 a         1437 c         1538 g         2686 t
ORIGIN      
        1 ttttgtcact ttttttctta gtcaaaaaaa gtaaccaaaa aagacaaggc aaaaccaaat
       61 gctccgctgg ttttgccaac ccacgcttct ttacatacac taaattttac ctttcgttgt
      121 ttttttattg aaaattataa ttttcaaacc gtacaacggg ttaaaaaaat gtatttactt
      181 agcagaaaat atatatattt actcatcaaa aacactactt atgaaattat tgatttctgc
      241 aattagctta ctgcttacac ttcacgtaca agcacaattt acaagtacaa acggtagcaa
      301 tgaaaaatat attgaagtta aaaccacaga tactttaatg gttaatcctg atgccatctc
      361 cttgttgatt gcattaggtg agaaaaaaga aaaaaactat tacttcgacg acgaccaaac
      421 tgatgcagaa aaagaagaag aagcgcgaaa ggaaaaaaat gatgccattc aaaagaaaaa
      481 acaaattgaa gaaatattga aaaaaaataa tgtttccgca tttaagtttc acgataaaaa
      541 agaggacaaa gacctattta ccaaagagtt taatctgtat gaaaatacat acgaagtgga
      601 attaaagaca gatgcacaag tagaaaaaat aaaaaaggag atgatcgtat tgaaagatgt
      661 gagcaccaaa gtggctaatt caaagctgaa tgataaagaa aaatacgaac tcatccttat
      721 cgataaagtg atgaagaagg cagagcgcga agcaagtgcc atcgcaaaaa ctatgaatgt
      781 aacattggac aaagcattaa atgtttcgaa tcaaagtgta aatgatattt attcttcaat
      841 gtttaacagt ccggaaagta tgggcggcat gggtgcctta ttttcaatga tgggaaatat
      901 gtttaaaggc gccacacaac aaaatacgca agtagttgtt tctaagacat tgattgttcg
      961 atttgcaata aaataaaatt atgttcaata aattcagcac agattacggc ttacttatta
     1021 tcagaatagg aattggtgtc atgtttgcat tacatggatt tccgaaaata actggtggtc
     1081 ccgaaaaatg ggaaatgatt ggcaaggcga tggaaaatat aggcatcggt tttgcgccaa
     1141 cattttgggg ttttatggct gcagtggcag agtttgtcgg tggcatctgt ttagtgttag
     1201 gttttctatt taggcctgca acagcgatgc tcttatttac aatgtttatc gcagcgttta
     1261 tgcattatgt aaatggtgat ggattttcgg gctattcgca tgctgttgaa agcgccatcg
     1321 tatttctagg attgtttttt tctggtcctg gaaaatatgc attaaaaatt tcgagacgat
     1381 aatctgttct cttaatttcc catatatttc atcaacacaa aaagcgatat cactgcaata
     1441 attgctaacg cgattttaat ggcgctgtat ggtcttgcgc cttgaacttc gcctgttctt
     1501 ccgtttatta taaactgaaa tacttttgag tgatagctgt atgcacttat ccaaataggc
     1561 agtaaaatat gcttgaaacg aatatcacta taggcaacat gcatattgtg aattcgctgt
     1621 tcgtcgccgc caatttctct gcgtatttta tcttttatgg cttcttccat caaatactta
     1681 gctgttttat agcctttgat gacatctgtt tgataacact cagtctgaaa accactaatg
     1741 tatgcttctt tgtatgcaac taaggcttct aaatcccaag gctcgagtgt tcgcatggta
     1801 ttatctggca acgactgact tgccgacaca caaacatcat cgaaagcatg ttgcgtagtg
     1861 ccttgtactg gataccattt ggttttacgt tgtttgttgt tgccggagcc ttcgtaataa
     1921 tattcaccac gttcgccatc ataactcgtg ttggtattag aatcgtaagt ccaataaggc
     1981 atatatacac cttttagttt ttcagaaact gatgcatatt tttttaagtc gtttggtgcg
     2041 aaccataatc catgtaacca ttttataaaa atatcgtgtg ctgttttatc atcgacttta
     2101 aaaggcagta tatattttgg ttttaatatt gttgaagtgg ttccgccttt aatcactaat
     2161 ggcgatgcac aaaaggcgca attatctgaa gtaatattcg gttttaaagt agttgttgcg
     2221 ccgcagttat tacaatttac cgtgtttaca gtttgtttat cttcttgctt gattttattg
     2281 gctataaaat catgatagtc catttcttca atttcctgat tttctacgtc tgcaagaatt
     2341 tcattttgtg cgccacaata tttacacgct aatttttctg tgccaggtgc aaaaagtagc
     2401 aatgcgccac aatctttaca ttgtagttca ttgcttatgt ttttattatt ttcagagaaa
     2461 gagtcgctca ttttttggtt ttatggtcga aagaaaaaag acttgacacg tttttgcttg
     2521 tgtcaagtcc tacatattgt tgaatatact cattacaatg gtggtggtgc caatggtggt
     2581 ggcaccgagt taagcactgc tgccagctct ggcaaagtag atgccgcagc ccatgctgcc
     2641 atgcccgctt tccacactaa ggtatccgct tttaattgtc cgctttgtgc catttgtgtc
     2701 aatgcaggca cgtcgaatgg tccggtttgt gcgccgttga cggctacaaa tatttgcatc
     2761 actggtggag gcactgcgcc gccgccttgt tgcgagccag caataccacc ttgcataatg
     2821 ccacctaagc ctacgccaac aaaagcacca gccaagccgc cgccattttc ggctgctttc
     2881 tcaatacctt ttgccgtttt ccattgtgtc aatttctgca tatcaatttt atccaaacga
     2941 gagagttcga aaatttcttt ttttacttcg tctggcattg atatattctc cactaatagc
     3001 gaagtcacct ttaagccata agcatcaaag tcatcggata atttttctgc gccgaacttt
     3061 gaaaactctt ctaagtttga ggcaaactcc tcaattttga gtttgctctc tgcaatagca
     3121 tctgtcagct tggtaaccac caaagcacgc aactgatttg cnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn ngttaaaatc ggcatgttgg cagtggttaa ttcatacctg
     3301 cctggctgat acacatcggc taatttacct tcatttaaaa acactgcaac ttgcgactca
     3361 cgaacggtga gttgtgcgcc tgttttaatt tcattctgaa atcgtggaaa tttccatacg
     3421 atcgtgtcgt ttgagttgtc tgtccattcg ataatgtcaa taaactcatt tctaaaagtg
     3481 tctaagattc ccattgtttt ggttttatta attttgatac aatttataaa aataaactca
     3541 tttcgagatt atataaacta ctatttttct ataaaagttc cgcaaattac tgtaataaat
     3601 agaaagtaat ctgtaataag tataacaaat tcgctacttt tggcttatta ataattccta
     3661 ctattttcat gaaaacaaca gcacttacag acacacacgt tgcgcttggc gcaaaaatgg
     3721 ttgactttgc cggattttat atgccagtac gttatacttc cgatttagac gaacatcact
     3781 gtgtgcgcaa tggcgtaggt atgtttgatg tttcacacat gggcgagttt attctaaagg
     3841 gtgagcaggc attgccatta ttacaaaaaa ttgtttctaa tgatatttca aaattagaaa
     3901 aagggcaggc gatgtatgga tatttgccca atgagcaagg aggaatcgtc gatgatttat
     3961 tggtgtatgc attagaagat ggtaattata tgtgcgtggt gaatgctggc aatatcgaca
     4021 aagattggaa ttggtttaat caacacaata cagaaaatgc tgaactacac aacatttcgg
     4081 aaaatgtggc actcttagca atacaaggac cgaaggcggc cgctgccttg cagtcgctta
     4141 ccgatattga tttatcatct ataccatact atcgatttaa gcgtggcgtg tttgctggag
     4201 tagaaaatgt tatcgtatcc aatacgggtt acacaggtgc tggtggcttt gagttgtatt
     4261 ttcacaaatc aaatgctgtt gctgtttgga ataaaataat agaagcaggc aaaccttatg
     4321 gattgcagcc aatcggacta ggtgcgcgcg ataccttgcg attagaaaaa ggattttgct
     4381 tatatggaaa tgacatcgac gacagcactt cacctttaga agcaggctta ggctgggtca
     4441 ccaaattcaa taaagatttt attgctaaag aaattttaca gcaacaaaaa acgaacggct
     4501 tatctaaaaa attagtcggc tttacaatgg tagatcgtgg tattcctcgt ggtcattata
     4561 tcattaaaga tgctcatggc aatccaattg gccaggtaac atcaggcaca caatcgccaa
     4621 cactcggcat tggcattggc ttaggatatg taaacataga atatagcacg atagatgcag
     4681 aaatctatat tgacattcgt ggtaaactat tgaaagcaaa agtggtaaaa ctgccgtttc
     4741 tataatctga agatattcta tcctatatga taaaaaatgc atttaaaatc agcatcgtct
     4801 ttacagtatt catattgttt ctgttttata ttgcaatcag tacggcatgg tttggaatct
     4861 ctactggtgt tggtggtgat ttttgcgaag ccgccagaaa tggcttgatt aagcagcccg
     4921 cgaactccta ttccaatatt ggatttttaa tttcagggtt ggcatgtgct tggatattga
     4981 gccataaaga tatagacaaa tcaggtgcct tttttaagca tccatttatt ccattgttct
     5041 attgtttaat caccatttta ttagggccat gttccatggc catgcacgca acagagacag
     5101 cactaggcgg attgttcgat atgaacagca tgtatttatt tggctctttt atgtttgcgt
     5161 ttgccttggc gcgttattac aaactccatt ctttcgtctt tgttatttta tattttagca
     5221 gtgtgttttt gtgtaattat gctggcacat tcaaaacagt atttggctat aacttcttcc
     5281 ctggaagtgc ggcatttggc gtagtgtgtg tgcttggtat gttgtttgaa atattgaatt
     5341 acaaaaagaa caaaccagat atacaattca aatatgcaat atattgttcg ctatcttttt
     5401 tagttggttt tggtgtatgg caatttggat atgatggtca ttgcttctgc aatccaaatt
     5461 ccttgtttca atggcatggt gtatggcatt tactgtgtgc tttatctacc tattttctat
     5521 ttcaatttta tatcagcgaa aaaacgcctg aatagcatga agtggctcgt gtgcattatt
     5581 attggtgtat tcggtataat tgcctgcaaa gaaaacaata ttaacacatc agcacctgat
     5641 gcttatttag gcaataccta tatcagccat cgatataaag acagattaag tgctgtgcaa
     5701 ttaattgaca gattaagtgc ggtttatcca aatgcagatt cgcttatacg tgcgcaatat
     5761 gatgtaacag tgtatcgtat tttttacaaa acacacaatt ttcaaaacca agaaattagc
     5821 gctagcggct tggtgtatat tccggaaatt cgacattatt ttgcgcctgt agtttgcttt
     5881 caacacggca catgcattca aaagcaagaa gtaccttcta ttgctgccaa tttaagttat
     5941 tatgtaccat tcatcattgc tagcgaaact ggcgctattg tttgtgctgc tgattatatt
     6001 ggcttaggat ttagtgatgg caagcatcat ttttatgagc ctacggaaga agcaaacgcc
     6061 gtggttgata tgctaggcag tgtgcagcct ttattaaata aaacctatca aaaaataaca
     6121 tttgatgata gtgtattgct atttggatat tcgcaaggtg gacatgcaac gcttgccgct
     6181 cagcgactgt tagaaacgaa atatgatgct cattttcaca ttcaagcaag tgcgccaatg
     6241 gcttcttggt tttcattcga aaattcatcg caattgaatg tgttaaaagc tgcgcttcct
     6301 tttgattttc ctagtgctta tgcttactta ataaatagta ttcaatcgac acaatctgta
     6361 ttttcatcgt ataacgaaat gctacaatca ccttatgatt ctttgtgtag cgttttattt
     6421 gatggcacaa aaagtactgg ctatgtcaac tcaaaatttc ctgctacatt tcatgcagca
     6481 ttacaaactt catttgtaaa tgagctcaat agcaatcctg ataataattt tatcaccgca
     6541 cttaaaaaat acgatattat caacgattgg attcctaaat cgtcaacacg tttttatcat
     6601 agccaaggtg atgaaattgc tttttatgac aatagtgaaa ttgcctataa cactttcaaa
     6661 aataaaggtg gtaatgttca tctaatcaat cttggaaatt acaatcactt tgaagcaaac
     6721 atcatcgcta ttgacagtgt tcgaaactgg ttttatccat taataaaaat taaaccgtac
     6781 tagctgttgc ttaaagcttt tatgtagttt gattatggta tgtattttat taacgcaatt
     6841 ttttttctca gatttgatgt atgagattcc cactttcgtg ggaatgacat ttgcgtggga
     6901 atgacgtttg cgtgtgaatg acaattttat gggaacgaca gttccctatt gctggtgttc
     6961 acaccaaaca acatttacaa cacagatttt tccttttgag aataaagtaa ttctaaccta
     7021 ttgtgtggtt taaggttgaa tttaaggtta aagcatttat ttttacagct cctttttaat
     7081 tatactgaat gatagattat gctgaacagt ttttacattc tgttgttatg tttatggtaa
     7141 tatgcaaagt aaaatgcagt atataaaaca cattgtttat gtagcagttt tgatttcaat
     7201 gtcatcggta atggcacagt caccgattgc tatttatgat ttgacgcaat atgaaaatcg
     7261 cattttacaa aaaaatgaca cattatatgt tgtcaatttt tgggctactt ggtgtaagcc
     7321 atgtgtgacg gaattaccat tttttgaaag taatgcgcaa cacttttctg agtttcctgt
     7381 taaaattatt ttggtgagcc aagatgccaa aacgcgcagt gcgcaagtca gcgatttttt
     7441 aaaaaagaat aattatacat cagaagcatt tattttgtcg gcaggtaatc cgaatatttg
     7501 gatagacaaa attgacaatc gttggacagg tgctattccg atgacacttt tttataaaaa
     7561 cggaaacaag gaatattttc atgaaggcga atattcgacc gctgaagaat tagaaagtta
     7621 tattcacact aaattataaa ttatgcaaaa agtacttatc accttattcg ctattattag
     7681 catttgtttt acgacacaag cacatcacgg ttatcatgtt ggtgataaca ttgctgattt
     7741 ttctttaaaa aacatagatg gaaaaaatgt gtcattggca aattatacga aagccaaagg
     7801 ctttataatt gttttcactt gcaaccactg tccctatgcc aaggcctatg aagaccgcat
     7861 tatcgcgtta gataaaaaat atgctgcttt aggttatcct gttattgcta tcaatccgaa
     7921 tgatgccgtg caatatccag aagatgacta tgctagcatg caacaacgcg caaaagaaaa
     7981 aggatttacg tttccatatt tacacgacga aacacaagct gtcacaaaaa cttttggcgc
     8041 tattaaaaca ccacatattt ttattgtgaa aaaagaaaac acaaacctca atttgaaata
     8101 tgtaggtaca attgatgata attgggaaga tgcaagtgca gtaacggaaa catttgtggt
     8161 caatgcagtt gatgaattat tagcagacaa gcctgttagt gttgctaaca caaaggctgt
     8221 aggctgtagt attaaatgga aaaaaccttg atagaaaatt gaaaaaaata agttactaag
     8281 attcccactt
//
LOCUS       DAOQQV010000117         8200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3414_length_8200_cov_8.395335, whole genome shotgun sequence.
ACCESSION   DAOQQV010000117 DAOQQV010000000
VERSION     DAOQQV010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3414_length_8200_cov_8.395335"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PLL59_10485"
     CDS             <1..1150
                     /locus_tag="PLL59_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HMY24277.1"
                     /translation="LKQKTHVGSRARIGIDAFFFSKLQLSLAIQDARTWGESSSTGVK
                     LLNNIFYEAWASYWFTQKVGIKIGRQNLSYDDERLIGGLDWAMQGRSFDALKGMFKLD
                     SNSNSQIELVVTYNNDNNKNNDTLGRTIYGISESGETTKSLQILHYKYKNDKKFQVSA
                     IVLNNVLQTKLGKYYDMFTIGVNVKKYYKKVGIYGSAYYQGGKNTLGQKKSAYQISFN
                     IDALFHDKFTTIFGTEFLSGRKYNTPSDWNLSFSPLYGTNHGFNGFMDYFYVGNHFNT
                     IGLNDYYIKTTTKIAAKSTLKLNFHAFTANRKMGFDNNGKKISPFLGSEIDAVFIQKI
                     GKIMTINIGQSFMINGNSMKLIKQVPNPKPVQSWTWVSLKFDPSVKLK"
     gene            1409..2617
                     /locus_tag="PLL59_10490"
     CDS             1409..2617
                     /locus_tag="PLL59_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMY24278.1"
                     /translation="MYSNIKIKREVYGSEEHIMLQKMCEQFIEKEITPYHAEWEEQGQ
                     VSREVWTKAGETGLLCLDVPSEYGGAGLDFSFCALITEVISKSGYTGPGFFLHSDIVA
                     PYIIDYGTEAQKHQWLPKMVSGEVITAIGMTEPSCGSDLQALKTTAVDMGDHYVVNGQ
                     KTFITNGYMSDLAIIAARTIIEGKDNGISLFLIESTREGYTKGKPFKKVGMKAQDTCE
                     LFFDNVQIPKDNLLGEIGKGFIYMMQKLSRERLSVALMSTGAAEGALENTIDYVSTRT
                     AFKQPIAAFQNTQFKLADMATKLQIHQTFIDRCVELYVKGELSAETASMAKSSCTDMH
                     FTLVDECLQLHGGYGYMWEYYIGRAYADNRVARIYAGTNEIMKLLIARGLFRELHEQQ
                     RQKKKAAVAN"
     gene            2662..4926
                     /locus_tag="PLL59_10495"
     CDS             2662..4926
                     /locus_tag="PLL59_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HMY24279.1"
                     /translation="MKYYLLFFSLFYICLARAQLVFTSNTTTPTSKLVLWYQQPALNW
                     MSDALPIGNGRIGAMIFGGVQQEHVQFNDKTLWKGNTTSFGAYQNFGDLFLDFPKTYT
                     VEDYIRELDLETAVSRVSYKANNIQYTRTYFCSYVDNAIIIHLSSDAANAISFNVSML
                     NAHKGTYSITGNKITFNGQLDLLAYEAQLTVKNDGGEISTLSNSLKIENANAVTLILT
                     LGTNYQPKSIGYTDINASQLSDTLTKQTNKVFSKPYNDLLRTHVADYKSLFDRVQLDL
                     GGMQTSVPTNILQKMYNDGDKNTALEELYFQYGRYLMISSARGIALPSNLQGIWNNSN
                     NPVWQSDLHSNINTQMNYWPAEITNLSELHSSLTDYIYNEAIIQASWQQLASSLGAKG
                     WALKTQNNIFGYSIWNWNRPANAWFSLHLWQHYLYTSDTGYLRQKAYPVMKSACEFWL
                     SQFTLGEEGKLLAPKEWSPEHGPWQDGVAYAQQLIWNLFNNTIKASKDLNIDNDFSAK
                     LQNKLEQLDNGIAIDKKGQIAEWKRYKPRRKDKKHRHISHLIALYPGNAISPLIDTLY
                     SNAAAHSLNNRGDAGPGWSRAWKINAWARLLNGERAYTLLRAALQNTTETGAKFNQAG
                     GAYQNLLDAHPPFQIDGNFGATAGIAEMLLQSHLEKIQLLPAIPMAWHSGVVKGLKAA
                     DNFTVDMEWNNSTLKRTTIYSGSGKKCALYYKNIHKAYIIDENKNKISFTVKHNNEIE
                     FETTVGKSYIIVMP"
     gene            complement(4999..6036)
                     /locus_tag="PLL59_10500"
     CDS             complement(4999..6036)
                     /locus_tag="PLL59_10500"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HMY24280.1"
                     /translation="MNEKLEFSTYKRIVQDFIGPIVCEISEIENGLINSTFLVRTVDK
                     KYILQKINTSIFKSPEILLRNYLKLNELLSNSSYSRQKITLLPSSLGDYIVKKQNNEH
                     WRLMEYIEQSKTFLKVPNPAIAYNAAACFSEFYKSINRQTLALEEPIDDFINFDKRIN
                     SYLLSLTTATAQRKETAKTIIDEVAPYLELHKKWDEHIQNGMFAKHNIHADPKISNIL
                     FNDANDAIAVIDLDTVMNASLLYDFGDMARSYCNLLEEDDCTQANNFSNEIYIAVKKG
                     FLSHLEDMLTPCELENLDYAAQLVVYIQAIRFLSDFLNGDVYYKTNYPTHNLDRAQNQ
                     FSLFSQMKNLL"
     gene            complement(6139..6828)
                     /locus_tag="PLL59_10505"
     CDS             complement(6139..6828)
                     /locus_tag="PLL59_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMY24281.1"
                     /translation="MDAIVPLISLIALEVILGIDNIIFISILSDRLPDAQRKSLRFWG
                     LGLAMVMRLLLLTFISWILKLDTTLFTAFDIDFSGKGIILIAGGLFLLYKSTKEIYHK
                     TEEPEAEELSVPKKSGFGRLLLEIILLDLVFSVDSIITAVGMVQELWVMYTAVVVTVG
                     IMLVASKPIGDFIKNHPSFKVLALCFLMMIGVSLIAEGMHFQIPKGYIYFSMAFAFFV
                     DIMQMKSEKRK"
     gene            complement(6882..7973)
                     /gene="carA"
                     /locus_tag="PLL59_10510"
     CDS             complement(6882..7973)
                     /gene="carA"
                     /locus_tag="PLL59_10510"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HMY24282.1"
                     /translation="MPTTPAILLLEDGTTFHGFACGKIGTATGEICFNTSMTGYQELF
                     TDPSYFGQLLVSTNVHVGNYGVKASEDESNQIQIAGLICRDYTNSFSRKQADSSIEDY
                     FIKNNLVGITGIDTRAVVRHVREKGAMNAIVSSENTDIDALKKQLATVPSMEGLELSS
                     KVTTKEAYTYGNENAKYKIAALDFGIKKNMLSNFDDRDCYVKVFPANTSYDELKKFEA
                     NGYFISNGPGDPSVMDYAVETVKQILVDDKPLFGICLGNQILARAVGIPTYKLHHGHR
                     GGNHPVKNLKTGKCEITTQNHGFAVDMEALKKSDKATLTHINLNDNSVEGLEIVGKKA
                     FSVQYHPEAAPGPHDSRYLFDDFINLMKQ"
BASE COUNT         2761 a         1431 c         1424 g         2584 t
ORIGIN      
        1 gttaaagcaa aaaacacatg taggcagcag agctcgtatt ggaattgatg catttttctt
       61 ttcaaaactg cagctttcat tggcaattca agacgcacgt acttggggcg agtcttcttc
      121 tacaggtgtg aagctattga ataatatctt ttacgaagct tgggcttcgt attggtttac
      181 gcaaaaagta ggcattaaaa ttggtcgaca aaatttatca tacgatgatg aacgtctgat
      241 tggtggctta gattgggcaa tgcaaggcag aagcttcgac gcactaaaag gtatgttcaa
      301 gttagattcc aattccaatt ctcaaattga attagtcgta acctacaaca atgataacaa
      361 caaaaataac gacaccttag gacgcaccat ttatggcatt tcagagagtg gtgaaacaac
      421 aaaatcctta caaatactgc attacaaata caagaacgat aaaaaatttc aggtgtcagc
      481 aattgtgttg aataatgtac ttcaaacaaa acttggtaaa tattacgata tgtttactat
      541 cggagttaat gtaaagaagt attataaaaa ggttggtatt tatggctcag cttattatca
      601 aggtggaaaa aatacactcg gtcaaaaaaa atctgcttat caaatttcat tcaatataga
      661 cgcattattc cacgataaat ttactactat ttttggtaca gaatttttgt ccggcagaaa
      721 atacaataca ccatctgatt ggaatctttc tttcagtccg ctttatggca ctaatcacgg
      781 ttttaatggc tttatggatt atttctatgt cggcaatcat tttaatacca ttggattaaa
      841 tgattactat ataaaaacta caacaaaaat cgcagcgaag tcaacactaa aactaaactt
      901 tcatgcattt actgctaata gaaaaatggg atttgacaat aatggcaaaa aaatatcacc
      961 gtttttaggg tcggaaattg atgctgtatt tattcaaaag attggaaaaa taatgaccat
     1021 aaatattgga caatccttta tgattaatgg caatagcatg aaattaatta aacaagtacc
     1081 taatccaaaa ccagtacaat cttggacttg ggtttcttta aaatttgatc cttctgtgaa
     1141 attaaaatag tgcctaagga agcttaacaa ttgctttagc aacatgaata ttctatttac
     1201 taatcgattt tattttagat atcgagcatc aatactatag aggctctgca atttttgcaa
     1261 agcttctata gctattttct caatagtatt ttcaagaagt gtttttactt gatttatcaa
     1321 agattttaat tcatacaaca caaactaaga cacctcactg tttagtagaa cgcaacaaat
     1381 tgcgtatatt tacacaaaac tgttaatcgt gtattcaaac atcaaaatca aaagagaagt
     1441 atatggttcg gaagaacata tcatgctaca aaaaatgtgt gaacaattta ttgaaaaaga
     1501 aatcacacct taccatgccg agtgggaaga gcaaggacaa gtaagccgcg aagtatggac
     1561 aaaagcaggc gaaacaggtt tgttatgttt agatgtaccc agtgaatatg gtggcgcagg
     1621 tttagatttt tcattctgcg cattaattac agaagtcatt tcaaaatcag gttatacggg
     1681 tccaggattt ttcttacatt ccgacattgt tgcgccatat attatcgact atggcacaga
     1741 agcacaaaaa caccaatggc tgccaaagat ggtgagtggt gaagtcatta ctgccattgg
     1801 catgacagaa cctagttgtg gcagcgattt acaggcatta aaaacgacag ctgtagatat
     1861 gggcgaccac tatgttgtaa atggtcaaaa aacatttatc accaatggtt acatgagcga
     1921 cttagccatc attgctgcgc gcactattat tgaaggtaaa gacaatggca tttcgctatt
     1981 tcttatcgaa tctactcgtg aaggctacac caaaggaaag ccgtttaaaa aggtaggtat
     2041 gaaagcacaa gatacctgcg aattgttttt tgacaatgtg caaattccaa aagataatct
     2101 gttaggagaa attggtaaag gttttattta tatgatgcaa aaactaagcc gtgagcgctt
     2161 gtctgttgca ctaatgagca caggcgcagc tgaaggtgca ttggaaaata caattgacta
     2221 tgtgagtaca cgtactgcat ttaaacaacc aattgcagcg tttcaaaaca cacaattcaa
     2281 attggctgac atggctacaa aactacaaat ccatcaaaca tttattgaca gatgtgttga
     2341 gttatatgta aaaggtgaat tgagcgctga aacagcatca atggcaaaat catcttgtac
     2401 agatatgcat tttactttag ttgatgaatg cttgcagcta catggtggtt atggttatat
     2461 gtgggaatat tatattggcc gtgcttacgc cgataatcgc gtagcgcgta tttatgctgg
     2521 taccaatgag atcatgaaat tattaattgc acgtggcctt tttagagaac tacacgaaca
     2581 gcaacgtcaa aagaaaaaag cagcagtagc aaattaacat caatagcatt cgagctattc
     2641 atgacaaatt aattacatga tatgaaatat tatttgttat ttttttcgct tttttatatt
     2701 tgtcttgcac gtgctcaatt agtatttact agcaacacta ctactcctac ttctaaattg
     2761 gtgttgtggt atcaacagcc tgctctcaat tggatgagtg atgcattacc tattggaaac
     2821 gggcgaattg gtgctatgat atttggcggc gtgcaacagg aacatgtaca gtttaatgat
     2881 aaaacattat ggaaaggtaa tacgacatca tttggcgctt atcaaaattt tggtgatttg
     2941 tttttagact ttccaaaaac atatacagta gaagattata tccgtgaatt agacttagaa
     3001 actgccgtca gtcgtgtaag ctataaagcc aataatatcc aatacacaag aacctatttt
     3061 tgtagctatg ttgacaatgc cataattatt catttgtcaa gtgacgcagc aaatgccatc
     3121 tcctttaatg tgtccatgtt aaatgcgcac aaaggcacct attctattac tggcaataaa
     3181 attacattta atggtcaact agatttattg gcttacgaag cacaattaac tgtaaaaaac
     3241 gatggcggcg aaataagtac actcagtaat agtttgaaaa tagaaaatgc gaatgcagtt
     3301 acactcatat taacacttgg tacaaattac cagcccaaaa gtattggcta tactgacatc
     3361 aatgctagtc aattaagcga cacactaaca aagcaaacta ataaagtatt ttcaaaacca
     3421 tataatgacc tgttaagaac gcatgtcgca gattacaaat cactttttga tagagtgcag
     3481 ttagacttag gcggaatgca aacaagtgta ccaacaaaca ttttacaaaa aatgtataac
     3541 gatggtgata aaaatacagc attagaagaa ctgtattttc agtatggtcg ttacctaatg
     3601 atatcgagtg caagaggcat cgcattacca tctaatttgc aaggcatttg gaacaatagc
     3661 aataatcctg tatggcagtc tgatttacat tctaatatca acacacaaat gaattattgg
     3721 cctgctgaaa ttacgaactt atctgaatta cattcatcac tgacagatta tatctataac
     3781 gaagcaataa tacaagcatc gtggcaacaa cttgcctcct ctttaggtgc taaaggttgg
     3841 gcattaaaaa cacaaaacaa catatttggc tattccattt ggaattggaa tcggccagcg
     3901 aatgcttggt tttctttgca tctttggcag cactatttgt acacgtcaga cacaggatac
     3961 ttacgacaaa aagcatatcc agttatgaaa agtgcctgcg aattttggct ttcacaattt
     4021 acacttggcg aggaaggaaa attactggca ccaaaggaat ggtcacctga gcatggacct
     4081 tggcaagatg gagtcgcata cgcacagcag ctaatctgga atttattcaa taacaccatt
     4141 aaggctagta aggatttaaa tatcgacaac gactttagcg ctaagctaca aaacaaatta
     4201 gagcaattag acaatggcat tgctatcgac aagaaagggc aaatagcaga atggaagaga
     4261 tacaaaccaa gaagaaaaga taaaaaacat agacacattt cacatctgat agcattatat
     4321 ccgggaaatg caatatcacc attaatagat actttgtatt caaatgccgc cgctcattct
     4381 ctaaacaata gaggtgatgc aggtccgggc tggagcagag catggaaaat aaatgcttgg
     4441 gctagattat taaatggaga acgagcatac acactgcttc gtgctgcatt gcaaaataca
     4501 actgaaacag gcgcaaaatt taatcaagca ggtggtgctt atcaaaattt attagatgca
     4561 catccacctt ttcaaattga tgggaatttt ggtgcgaccg ctggaattgc agaaatgtta
     4621 ctccaaagtc atttagaaaa aatacagttg ctgccagcaa ttccgatggc atggcattct
     4681 ggtgttgtaa agggtttaaa agctgctgat aattttactg tagatatgga atggaataat
     4741 agcacattaa aacgtacaac aatttattct ggctcgggaa aaaaatgcgc actatactat
     4801 aagaacatac ataaagctta tataatagac gaaaacaaaa ataaaatttc atttacagta
     4861 aaacataata atgaaattga attcgaaacg acagtaggaa aatcctatat catagttatg
     4921 ccatagttta tttttgcaat caattatcaa cttttattta tgtatgtaag catttctaaa
     4981 aacagacaca ttgattattt aaagtaaatt cttcatttga ctaaataaag aaaattgatt
     5041 ttgcgctcta tctaaattat gcgtcggata attggtctta taataaacat cgccatttaa
     5101 aaagtctgac aaaaatctga tcgcttggat atatacgacg agctgtgcgg cataatctaa
     5161 attttctaat tcacaaggcg tgagcatatc ttctaaatgc gataaaaaac cttttttgac
     5221 agcaatatat atttcattgc tgaagttgtt tgcttgtgtg caatcatctt cttctaatag
     5281 gttgcagtat gagcgtgcca tatctccaaa atcgtagagt aagctcgcat tcattactgt
     5341 atctaagtcg attactgcaa tcgcatcatt agcatcatta aataaaatgt ttgaaatttt
     5401 tggatcagcg tgaatattat gtttggcaaa cataccattt tgtatgtgct catcccattt
     5461 tttatgcagt tctaaatatg gagccacttc atcaataatc gtcttcgctg tttcttttct
     5521 ctgtgcagtt gccgtcgtta aagaaagcag gtagctgtta attcttttat caaaattaat
     5581 aaaatcatct attggctctt ctaaagctaa agtttgtcta tttatcgatt tataaaattc
     5641 gctaaaacat gccgctgcat tataggcaat cgctggattt ggaactttta gaaatgtttt
     5701 agattgctcg atatattcca tcagtcgcca atgttcattg ttttgctttt ttacgatgta
     5761 atcacctaaa ctgcttggta gtaatgtaat cttctgccta ctataactgc tgttagataa
     5821 gagttcatta agtttcaaat aattacgtaa aagtatttcc ggtgatttaa atattgacgt
     5881 atttattttt tgtagaatat atttcttatc tactgtgcgc actagaaatg tagaattgat
     5941 gagcccgttt tctatttctg aaatttcaca tacaattggt ccaataaaat cctgtacaat
     6001 ccttttgtat gttgaaaatt cgagtttctc gttcatttaa cgtaaatata cttttttttt
     6061 gaaaccccta aaataaaagt gcaacattag tattatagac ttgctcgaag tagctaaaag
     6121 tgctatgtat taagaagctt atttgcgttt ctcagatttc atctgcatga tatcgacaaa
     6181 aaatgcgaat gccatagaaa aataaatata gcctttcggt atttggaagt gcataccttc
     6241 tgcaattaag gaaacaccaa tcatcattaa gaaacataaa gctaatacct taaatgatgg
     6301 atggttttta ataaaatcgc ctattggttt tgaagctacc aacatgatac ctacggtaac
     6361 aaccacagcg gtgtacataa cccaaagttc ttgcaccata ccaacagcag ttatgataga
     6421 atctactgag aaaactaaat ccaatagtat gatttcgagc aacaatctac caaacccact
     6481 ttttttaggt acagataatt cttccgcttc tggttcttcc gttttatggt atatttcttt
     6541 tgtgctttta tataataaga aaagtccacc cgcaattaag ataattcctt tgccagaaaa
     6601 atcgatatca aatgcggtaa ataaagtggt gtctaatttc aatatccaag atataaatgt
     6661 caatagtagt aagcgcatca ccatggccaa accaagaccc caaaaacgca aactttttct
     6721 ttgagcatca ggcaaacggt cggataaaat ggaaatgaaa atgatattat ctatacctaa
     6781 gataacttcc aaggcaatca atgatattaa aggtactatt gcatccatat actaagttga
     6841 aatactattt ttaaaattga aaggtaaaaa ataatgtttg attattgctt catcaagttg
     6901 ataaaatcat cgaataaata acgtgaatca tgtggtccag gcgcagcttc tggatgatat
     6961 tgaacagaaa atgctttctt gcctacaatt tctaagcctt ccacagaatt atcattgaga
     7021 ttgatatgtg tcaacgttgc tttgtctgat tttttcaagg cttccatatc aacagcgaag
     7081 ccgtggtttt gtgttgtaat ttcacatttt cctgttttca aattttttac aggatgattg
     7141 ccgcctcgat gtccgtgatg tagtttataa gtaggaatac caacggcacg agctaatatc
     7201 tgattgccta agcaaatacc aaataatggt ttgtcgtcta ctaatatctg ttttactgtt
     7261 tctaccgcat aatccattac acttggatcg ccaggtccat tagaaataaa atagccattc
     7321 gcttcaaatt tttttaattc atcatacgaa gtatttgctg gaaatacttt tacatagcaa
     7381 tctctatcgt caaaattact aagcatattc tttttaattc caaaatctaa agccgcaatt
     7441 ttatactttg cattttcatt gccgtaagta tatgcttctt ttgtagtcac tttagaagaa
     7501 agctctaaac cttccatgct cggtactgtt gccaattgtt ttttcaatgc atcaatatct
     7561 gtattttcgg aagatacaat tgcattcatt gcgccttttt cgcgtacgtg gcgcaccaca
     7621 gcgcgtgtgt ctatgcctgt aattcctact aagttatttt tgataaaata atcttcgata
     7681 gaactgtctg cttgttttcg tgaaaagctg ttggtataat ctctacagat aagacctgca
     7741 atttgaatct gattggactc atcttcagaa gctttaacac cataatttcc aacgtgtaca
     7801 ttagtagaaa cgagcaattg accaaagtaa cttggatctg taaacaactc ttggtagcct
     7861 gtcattgatg tattaaagca gatttcgcca gttgcagttc cgatttttcc gcaggcaaat
     7921 ccgtggaatg ttgttccatc ttctagcaat aatatggcag gtgtagtggg catgattatg
     7981 attttaaaaa attaaaattt tacacttcaa aattaaaaaa aaggatagct aaaaaaagct
     8041 atccttaatt atataacgaa aaaatcattt cttattcttc tgttttttct tcagctgatg
     8101 ccgttgcctc ttcttttact tcttctacct tctcttccac tacttgcgct gcttccgatt
     8161 tttttgctgc gccagaacga cgtgtttttt tcttaggttc
//
LOCUS       DAOQQV010000118         7858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3651_length_7858_cov_6.907343, whole genome shotgun sequence.
ACCESSION   DAOQQV010000118 DAOQQV010000000
VERSION     DAOQQV010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3651_length_7858_cov_6.907343"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..503
                     /locus_tag="PLL59_10515"
     CDS             132..503
                     /locus_tag="PLL59_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMY24283.1"
                     /translation="MDIFAGSLPFKIKEKELQILFEQFGEVSSVKIIIDKITRQNKGF
                     GFIVMPNDGEALNAIEALDGSEVMGRQITVSMSEEKKASSGKKKNFGKGGSNFKGDFD
                     KAKKSFPGKSGFQRGASRGRR"
     gene            complement(573..2351)
                     /gene="sppA"
                     /locus_tag="PLL59_10520"
     CDS             complement(573..2351)
                     /gene="sppA"
                     /locus_tag="PLL59_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMY24284.1"
                     /translation="MRSFLKTFFACLLAFGCLFFFIFVFLIGGLLSSGEKTVKTESNS
                     ILNLTLKGEITDRAYSNPFRNISPMAALSGSISDASTIGLFELKDVLEYAKKDDHIKG
                     IYLNLDEIDARPATMFELRKAIEDFKTSKKLVYAYSNATPEINLLVNTVADKFYINPV
                     GGCDFDGFAMQMDYLKGLMDKLGIDFKVFYVGEFKSATESYRRADMSPEDRLQRTELL
                     NDISGDYLNQVAQSTKVGVDSLKAMQNNLSVMDAASAVQYKLANALRYEDEVKDELKE
                     RLGYKKSDDLNMISYNDYKSIVKDKTTKSNDNKIAVLFAEGEINDSKSDDGVVGGEAY
                     VKTIQKLTADKSIKGVVLRVNSPGGSAFASEQIHHALMQLKAKKPLVVSMGDYAASGG
                     YYIAAPADKIFAQANTITGSIGIFGLIPNIRKALNEKVGVTFDEVKTAEHANFFNMIN
                     DWDELETSTVQKHLEKGYATFLDRVAKGRKMDTAAVNKVARGRVWTGQDALTIGLVDE
                     IGGLDEAIIAVKKLAKITAAEQVNYPKSKSVMESIMSDLLGEKENEQTKLLKNITPQL
                     LFFKQLERFQSLGVYQYRLPFDMQVR"
     gene            2431..2916
                     /gene="folK"
                     /locus_tag="PLL59_10525"
     CDS             2431..2916
                     /gene="folK"
                     /locus_tag="PLL59_10525"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HMY24285.1"
                     /translation="MHSVYLLLGSNKGHRRKHLLLAAYFIQQLAGKIEKTSAVYETEP
                     WGKSNQPKYLNQALLISTTKTPLQLIRILQKIEKKLGRTNKNHFAARTIDIDILFYDS
                     IVLASKKLIIPHPRLHLRNFTLQPLSELNKSYTHPVLQKSIDDLAKHCVDTLKVSIFK
                     Q"
     gene            2942..3610
                     /locus_tag="PLL59_10530"
     CDS             2942..3610
                     /locus_tag="PLL59_10530"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMY24286.1"
                     /translation="MIYKFISIEGNIGAGKTTLASLLAEEFHGQLILEAFEDNPYLPK
                     FYEDNNKYALQLEMSFLIERYQQLTRMFSEPNIFSNFTVTDYMLKKCLLFAKVNLSKQ
                     EYKLYKIFFDQVYKKLPKPEIIFYLHADTDQLMRNIRKRGREYEQNMSRIYLNRLEKM
                     YFEFFKQNPENKYVIIDVNGIDWISNVFAYRQLLKLFDREYAVGLNFVKLEQEALLHV
                     DASV"
     gene            complement(3662..4195)
                     /locus_tag="PLL59_10535"
     CDS             complement(3662..4195)
                     /locus_tag="PLL59_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HMY24287.1"
                     /translation="MRKLKLDELNRLSTAAFKAADKIPLIIVLDNIRSAANIGSVFRT
                     CDAFLVEAIHLVGICAKPPNRDIQKSALGATETVDWQYFEHIKDSIADLRKNGFEILT
                     VEQSDQSTMLHDFQPKTDKKYALIFGNEVEGVSDFAIQYCDTCIEIPQFGMKHSLNIA
                     VSAGIVIWDFARKIKLD"
     gene            4223..6907
                     /gene="mutS"
                     /locus_tag="PLL59_10540"
     CDS             4223..6907
                     /gene="mutS"
                     /locus_tag="PLL59_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMY24288.1"
                     /translation="MEKQAIAPRSCFHIFANQKTKIDKIVAKEKEAKETPLMAQYNKI
                     KQKYPDAILLFRIGDFYETFGSDAIKASQILGIVLTKRANGNQTELELAGFPHHSVET
                     YLPKLVKAGYRVAICEQLEDPKMVKGIVKRGVTELITPGVAINDKILDHKSNNFLASI
                     YYNENNVGIAFIDISTGEFLVAEGDENYIDKLLQSFAPAEIIFPKYQQKKFVATYGNK
                     YYTFALEDWIYQDDFLKDKLHQHFQVQTLKGFGVDEMPLGTQAAGACLYYLAETEHNK
                     LQHIASISRIEHDKYVWIDRFTARNLELIQSTHEKGVPLIQIIDKTISPMGSRLLKRW
                     IVLPLKDKQHINERLDAVEYLIKENDTFQLIIHHIKQIGDLERLISKVPLGKINPREV
                     IQLKRALLAIQPIKETCLASENNGLKKIGEQLQLCQLIKEKIEHTLQEDAPVAIQKGN
                     AIKAGISETLDELRSLSSSGKNYLLEIQNRESERTQIPSLKIGFNNVFGYFLEVRNTH
                     KDKVPNDWIRKQTLSGAERYITPELKEYEEKILGADEKILALEVKLFEELVSEILDYI
                     QPIQRNANLIARLDCLQSFSSIAVKHHYRRPVIADDLVLMIKEGRHPVIEQQLPLGEQ
                     YIPNDVYLQPDEQQIIIITGPNMSGKSAILRQTALIVLMAQIGSFVPAQEAHIGIVDK
                     IFTRVGASDNLSLGESTFMVEMIETASIVNNISERSLVLLDEIGRGTSTYDGISIAWS
                     LVEFLTKNKIAKPKTMFATHYHELNELAEKFHRIKNYNVAVKESGNKVIFLRKLIQGG
                     SQHSFGIHVAKMAGMPNELIKRANEILIELESQRNLASDVSSSLKNLAIKEDFQLNMF
                     AMDDPRLLKLKEVLEKVDINSMTPVEAMMKLDELRKLL"
     gene            7144..7216
                     /locus_tag="PLL59_10545"
     tRNA            7144..7216
                     /locus_tag="PLL59_10545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7177..7179,aa:Gly,seq:gcc)
     gene            7263..7346
                     /locus_tag="PLL59_10550"
     tRNA            7263..7346
                     /locus_tag="PLL59_10550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7296..7298,aa:Leu,seq:taa)
BASE COUNT         2797 a         1339 c         1278 g         2444 t
ORIGIN      
        1 gaattcggca gaatattaca aaccataaaa gcttaattgg ttgatttttt ttgaagtgtg
       61 tgtcccgagt ttatctcggg atttttattt aataaaaata aaagaactac ctttatacta
      121 aattaatcaa tatggatatt tttgctggca gtttgccatt taaaataaaa gaaaaagaac
      181 tacaaatttt atttgaacaa tttggtgaag tgagttctgt aaaaattatc atcgataaaa
      241 ttacacgaca aaataaaggc tttggtttta tagtgatgcc gaatgatggc gaagcactta
      301 atgcaatcga agcgcttgat ggtagcgaag taatgggaag acaaattaca gttagtatgt
      361 cagaagaaaa aaaagcatca tcaggaaaga agaaaaactt tggtaaaggt ggctccaact
      421 ttaaaggaga ttttgacaaa gcaaaaaaat catttccggg aaagagtggc tttcagcgtg
      481 gtgcatcaag aggtagaaga tagaagataa aaaataaaaa atcgcattgg aattccaatg
      541 cgatttttta tttagtaaaa tataaattgc aattatcgaa cttgcatatc aaatggtaaa
      601 cgatattgat aaacacctaa tgattgaaag cgttctaatt gtttgaaaaa taacaattgt
      661 ggcgttatgt ttttcaataa ttttgtttgt tcattctctt tctcgccgag caaatcgctc
      721 attatacttt ccataactga tttgctttta ggataattta cttgttcagc agctgtgatt
      781 ttagccaatt ttttaacggc aataatcgct tcatctaagc ctccaatttc atcaactaaa
      841 ccaattgtta aagcatcttg gcctgtccac acacggccac gtgctacttt gtttactgca
      901 gcagtatcca tttttctacc tttggccact ctatctaaaa aggtagcata gcctttttct
      961 aaatgctttt gcacggtaga tgtttccagt tcgtcccaat cattaatcat attgaaaaaa
     1021 ttggcatgtt ctgccgtttt tacttcgtcg aaagtaacgc ctactttttc atttagtgct
     1081 tttctgatat tcggaataag gccgaaaata ccaatagaac cagtaattgt attggcttga
     1141 gcaaatattt tatctgctgg cgcagcaata taataaccac cagaagcagc atagtcgccc
     1201 atagaaacga caagtggttt tttggcttta agctgcatta aggcatgatg aatttgctca
     1261 gaggcaaacg cacttccacc tggcgaattg acacgcaaca caacaccttt tatactttta
     1321 tctgctgtta atttttgaat cgttttaaca taagcttctc cgccaacaac accatcatct
     1381 gatttgctat cattgatttc tccttctgca aaaagcaccg caattttatt atcattactt
     1441 tttgtcgttt tatctttcac aatagattta taatcgttat acgatatcat atttaaatca
     1501 tcgctttttt tatagcccaa tctttctttc aactcatctt ttacttcatc ttcataccta
     1561 agcgcattgg ctaatttata ttgcactgcc gacgctgcat ccatcacaga aagattattt
     1621 tgcatggctt tcaaagaatc aacgccaact tttgtcgatt gagcaacttg attcaaataa
     1681 tcaccagaaa tatcatttaa taattcagtg cgttgtagtc tatcttctgg gctcatatca
     1741 gcgcgtctgt aactttctgt tgcgctttta aattcaccta cataaaacac tttaaaatcg
     1801 attcctaatt tatccatcaa gccttttaaa taatccattt gcatagcaaa accatcgaaa
     1861 tcgcagccac caacaggatt gatataaaat ttatctgcaa cagtattcac taacaaattt
     1921 atttcaggtg ttgcattgga ataagcatat actaatttct tggaggtctt aaaatcttca
     1981 atagccttgc gtagctcaaa cattgttgca ggtcttgcat ctatttcatc taaatttaga
     2041 taaatgcctt ttatatggtc atctttttta gcgtactcta acacatcttt cagctcaaat
     2101 agaccaatcg ttgatgcatc agaaattgag ccactaagcg cagccatcgg actaatattt
     2161 ctaaacggat tactataagc tctatctgtt atctcgcctt ttaaagtcag attcaaaata
     2221 gaattagatt cggttttaac tgttttttct ccactactca ataagccgcc aattagaaaa
     2281 acaaaaatga agaaaaacaa gcagccaaat gccaataagc acgcaaaaaa tgttttaaga
     2341 aaacttctca tgaaaaatat ttaaaataat taatataaaa atggtttaaa aagttcaaaa
     2401 ttagttgtta ttattggaaa aatattactc ttgcactcag tttatttatt attaggctca
     2461 aataaaggtc atcgcagaaa acatttgcta ttggcagctt attttataca acaattagct
     2521 ggaaaaatag aaaaaacttc agctgtgtat gaaaccgaac cttggggaaa atcaaatcag
     2581 ccaaaatatc tcaatcaagc attgttaatt tctactacaa aaacaccttt gcagcttatt
     2641 cgaatcttac aaaaaataga aaaaaaactc ggcagaacaa ataaaaatca ttttgctgca
     2701 agaaccatag atattgatat tttgttttat gatagcattg tactcgcatc taaaaaattg
     2761 attattccgc atccaaggct acatttacgc aatttcactt tacaaccact ttccgagtta
     2821 aataaaagtt atacacatcc tgttttgcaa aagtcaatag atgatttggc aaagcattgt
     2881 gtagatacac ttaaagtcag tatttttaag caataaaaag cacgtaagtg tacgtatcaa
     2941 aatgatatat aaattcatca gcatagaagg aaatattggc gcaggaaaaa ctaccttagc
     3001 ttcgctatta gcagaagaat ttcatggcca attgattcta gaagcatttg aagacaatcc
     3061 gtatttacca aaattttacg aagacaataa taaatatgct ttacaattag aaatgtcatt
     3121 tttgattgaa cgctatcaac agctaacgcg aatgtttagc gaaccgaata tattttcaaa
     3181 tttcactgta acagactaca tgctcaaaaa gtgcttgtta ttcgctaaag tcaacttgag
     3241 caagcaagag tataaattat ataaaatctt ttttgatcaa gtatataaaa aattaccaaa
     3301 acctgaaatc attttttact tacatgccga caccgatcag ctgatgcgca atatcagaaa
     3361 acgaggcaga gagtatgaac aaaatatgtc gcggatttat ctcaatagat tagagaaaat
     3421 gtatttcgaa tttttcaaac aaaatccaga aaataaatac gtcattattg atgtcaatgg
     3481 tatcgactgg ataagcaatg tatttgccta tcgccaattg ttgaagctgt ttgacagaga
     3541 atatgcggtc ggcttaaatt ttgtaaaatt agaacaagaa gcattattac acgttgatgc
     3601 atctgtataa tttgcggatt acgctataat cgacttttga tttatttaaa cacacaatgg
     3661 attaatctag tttaattttt cttgcaaagt cccaaattac gatgccagca gaaacagcaa
     3721 tattcaaaga atgtttcatg ccaaattgcg gaatttctat gcaagtatca caatattgaa
     3781 tggcaaaatc agaaacacct tctacttcat taccaaaaat caaagcatat tttttatctg
     3841 ttttgggttg aaaatcgtgc aacattgttg attgatcaga ttgctcaaca gtcaatattt
     3901 caaaaccatt tttacgcaag tcagctatac tgtctttaat atgttcaaaa tattgccaat
     3961 cgacagtttc cgtagcgcct aaagctgatt tttgaatatc gcgattcggt ggcttggcgc
     4021 aaatacctac caaatgtatt gcttcaacta aaaacgcatc gcaggttcga aaaacggagc
     4081 caatattagc agcacttctt atattatcta atacaataat caatggtatt ttatcggctg
     4141 ctttaaatgc tgctgtggat aatctattga gttcgtctaa ttttagttta cgcataaatt
     4201 gtatttttac atcattgtaa gattggagaa acaagcaata gcacctcgtt cttgcttcca
     4261 tattttcgca aatcaaaaaa caaaaataga taaaatcgtg gcaaaagaaa aggaagcgaa
     4321 agaaacgcca ttaatggcgc aatacaacaa gataaaacaa aaatatcctg acgctatttt
     4381 attatttcgt atcggcgatt tttatgaaac ttttggcagt gatgccatca aagcatcaca
     4441 aatattaggc attgtactga caaagcgcgc caatggcaat caaacggaat tagagctggc
     4501 tggttttccg catcattcgg tagaaactta tttacctaag ttagtgaaag caggctatcg
     4561 cgtagccatc tgtgagcaat tggaagatcc aaagatggtg aaaggcattg taaaacgtgg
     4621 tgtcacagaa cttattacgc ctggcgttgc tattaatgat aaaatattag accacaaatc
     4681 caataatttt ttagcttcta tctattacaa cgaaaataat gtaggcatcg catttataga
     4741 tatttcaact ggtgaatttc tagtagcaga aggcgatgaa aattatatcg ataaattact
     4801 gcaaagcttc gctcctgctg agattatttt tccaaaatat cagcaaaaaa aattcgttgc
     4861 cacatatgga aataagtatt acacatttgc tttagaagat tggatttatc aagatgattt
     4921 tttaaaagat aaactacatc agcattttca agtacaaact ttaaaaggtt ttggtgtgga
     4981 cgaaatgcca ctaggcacac aagctgccgg tgcttgctta tattatttag ctgaaacaga
     5041 acataataag ttgcaacata ttgcttctat ttctaggata gaacacgata aatatgtttg
     5101 gatagataga tttacggcaa gaaatttaga gctaatacaa agtacgcacg agaaaggtgt
     5161 gccactgata caaattatag ataaaaccat ctcgccaatg ggcagccgtt tgttgaaacg
     5221 ctggattgta cttccattaa aagataaaca acacatcaac gaacgcttag atgccgtcga
     5281 atatttgata aaagaaaatg acacgtttca acttatcatt catcacatca agcaaatagg
     5341 cgatttagaa agattaattt ctaaagtgcc attaggaaaa ataaatccac gcgaagttat
     5401 acaacttaaa cgcgcattac ttgccattca accaataaaa gaaacatgtc ttgcctctga
     5461 aaataatggc ttgaaaaaaa ttggagagca attgcaatta tgtcaactta taaaagaaaa
     5521 aatagaacat acactgcaag aagatgcacc tgttgcaatt caaaaaggca atgcgataaa
     5581 agcaggtatt tctgaaacat tagatgaatt aaggagtctg tcttcctcag gaaaaaatta
     5641 tttattagaa attcaaaata gagaaagtga gcgcacacaa attccttctt taaaaattgg
     5701 atttaataat gtatttggat actttttaga agtacgaaac acgcacaaag acaaagtgcc
     5761 taatgattgg atacgcaagc aaacattgag tggtgcagaa agatacatca caccagaatt
     5821 gaaagaatat gaagaaaaaa ttttaggtgc tgacgaaaaa attcttgcgt tagaagtcaa
     5881 gttgtttgaa gaattagtat cggaaatttt agattatata cagcctattc aaagaaatgc
     5941 aaacttgatt gctcgtttag attgtttgca atctttcagt agcatcgccg taaagcatca
     6001 ttacagaagg cctgtaattg ctgatgattt ggtgttaatg ataaaagaag gacgacatcc
     6061 tgttattgaa cagcaattgc cattaggtga gcaatatatt ccaaatgatg tatatttaca
     6121 gccagatgag caacaaatca tcatcattac cggaccgaat atgtctggta aatcagccat
     6181 tctacggcaa acagcattaa ttgtattgat ggcacaaata ggaagtttcg tgccagcaca
     6241 agaagcacat atcggtattg ttgacaagat ttttacacgc gttggcgcca gcgataatct
     6301 gagtttaggc gaatctactt ttatggtaga aatgatagaa acagcgagta tagtcaataa
     6361 tatttcagaa agaagcttag tattattaga tgaaattggg cgaggtacaa gtacttacga
     6421 tggcatttct attgcttgga gtttggtaga atttttgaca aaaaataaaa tagcaaaacc
     6481 caaaaccatg tttgccactc attaccatga gttaaatgaa ttagcagaaa aatttcatcg
     6541 cataaaaaat tacaatgtag ctgtaaaaga aagtggcaat aaagtaattt ttttacgtaa
     6601 attaatacaa ggaggctcac aacacagctt tggtattcat gtagcaaaaa tggcaggcat
     6661 gccgaatgaa ttaataaaaa gagctaatga aatattaatc gaattagagt cacaacgtaa
     6721 tttagcaagc gacgtcagtt cctctctcaa aaacttagct attaaagaag actttcagtt
     6781 gaatatgttt gccatggatg atcctagatt gcttaaatta aaagaagtac tcgaaaaagt
     6841 agatatcaat tccatgacgc ccgtagaagc catgatgaag ttggacgagt tgcgaaaatt
     6901 attataaaac aacaaagtag aatatcttaa aattatttcc ttatttattg caaacctatt
     6961 tttatttctt gttgcagtaa tgaatgaact tagacttgaa ataggcgaat tgatggtata
     7021 ataaaaagct acttaacgaa attgttacga aataaaaaaa aacacaatta cacttgtcaa
     7081 aaataaaaag attactatct ttgcattccg ttagcaaaca gctaatcgtt ctttaaaata
     7141 caagcgaaag tagctcagtt ggtagagcat caccttgcca aggtgagggt cgcgggttcg
     7201 agcctcgtct ttcgctctat tcttttaaaa ggagtttccc aactcctttt ttgttttaca
     7261 gcgcccgggt ggtggaatgg tagacacgca ggacttaaaa tcctgtggcc atcgcggctg
     7321 tgcgggttcg agtcccgccc cgggtacgct tttatttttt ctttttctcg atagcgcaaa
     7381 aaaaaagtat agatttaata ctatactatc tttattaata ataaatatta ttactcataa
     7441 aataatacca taaaattggt attcattttt atatattttc acatttttaa caagctactc
     7501 attaataggt atattagtac ttttttgttg atttacatca aatatttaac aatttagaaa
     7561 ctttaggctt gacatacata aaaaaaaata ctaatattac atcaataacg caactaacga
     7621 gtcacccgat gtcccagaaa ggatagacat aaaaaccccc tcagaaatat gaaaccccta
     7681 aaattagggg tttttgtttt ttaatacatt tcaatcaaaa tcaaacatct atacattcaa
     7741 attgatatca atcaataaaa tgtcgaaata caattattta tttgcttttt taaaaacttt
     7801 acaatatctt gaacataata tttaattaac gcacgcttta ttctattatt aaccaaaa
//
LOCUS       DAOQQV010000119         7841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3660_length_7841_cov_6.905086, whole genome shotgun sequence.
ACCESSION   DAOQQV010000119 DAOQQV010000000
VERSION     DAOQQV010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3660_length_7841_cov_6.905086"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLL59_10555"
     CDS             <1..357
                     /locus_tag="PLL59_10555"
                     /inference="COORDINATES: protein motif:HMM:NF036524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24289.1"
                     /translation="RNEDKYITIIKKNRFPFIQNAVFSELKSNFYVYVDGLILTASNS
                     GEIQIRPFLSNYYKQVIWNAFLSILYFWSNDKSNFKENTDVIVEKTIHFAFDLLAPNA
                     IDSGIDYLQHFIKLRK"
     gene            367..1686
                     /locus_tag="PLL59_10560"
     CDS             367..1686
                     /locus_tag="PLL59_10560"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HMY24290.1"
                     /translation="MMEEQEKIPTSKVARASSVFRTSMRVGANYLKHNAQKMVHKEVN
                     EDALNEKNASELFNLMSQLKGSALKIAQMLSMDNGLLPKGYAEKFALAQNSAMALSSP
                     LVMNTFMKYMNQSPYDLYDSFEIKANHAASIGQVHLAYKNGNKLAVKLQYPGVADSIH
                     ADIQMVKPLLLRIVGIKEIEVKQYFDEFEERLIDECNYQLELSNGVEIAAATKHIPNL
                     IIPTYYPALSSSKVLTMEWIDAQPMQVFLDTETNQANKDKIGQALLDFLHTSIHDLKK
                     FHADPHPGNFLVTKDMQLVVLDFGCVKSIPNDFYNYYFSLVRNDVLADTDKLRICLKG
                     LDMLRDSDTAATDQLFMETSLKAIQCLSKPMKSDTFYFGDLAYYEQLQLQGEEFMNNK
                     ALRTPEAMRGSRHAIYLHRTFFGLYSILHKLNATVRIDRRFIENVGV"
     gene            1748..2149
                     /locus_tag="PLL59_10565"
     CDS             1748..2149
                     /locus_tag="PLL59_10565"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMY24291.1"
                     /translation="MKKLDASIVIYNSMLSDSEQEVANVLANKINQLLPNAKQKIWHS
                     HPVWFINDNPIVGYSKQKLGIRLMFWSGADFVQHKFTVIGKKFKDASIFYKTLSEINY
                     SDLENWILQAKAIQWDYKNIVKRKGLLLRLL"
     gene            complement(2150..3577)
                     /locus_tag="PLL59_10570"
     CDS             complement(2150..3577)
                     /locus_tag="PLL59_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY24292.1"
                     /translation="MDKADKLYADYKYAQAIELYKPIAEKGNIKAIRKIAECYRKIND
                     YQQAEKYYALVTADKNAIPKSYLYYGQMLLANEKYEDAKIWLQKYLTLKNDADTSIAR
                     TLLLSCEQQKNNPTSARKIEVQNMKALNSIHADFCAVPYDNGILFTSARQGKINGTSG
                     SGFQEVYYAFYNADSTMTVEPVKGVVNTKNYNSGPACVDTLHDIIYFTKNNFQYGDAV
                     TNKKGDVTLKLFSAKKSKDGWKDIKELELNDKEYSCAFPSINTEGNMLFFTSDRGGGF
                     GGKDIYYSTYQNEKWSKPKNAGKEVNTKGDERYPFIHHDGTLYFSSTGLPGFGGMDIF
                     KCVPNKLGEFGKPENLGKPFNSATDDFGFYIDANYSNGFFSSDRSGGMGSDDIYSFQF
                     KDIAFELNVYCDSKLADAVQLSVQENDSLIKTIEVNKKIAFILQPNTKYTFTCTKDKY
                     TQEQLIVKTTSNKKPINKSINLTAK"
     gene            complement(3665..4504)
                     /locus_tag="PLL59_10575"
     CDS             complement(3665..4504)
                     /locus_tag="PLL59_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMY24293.1"
                     /translation="MKLYTIDTGFFKLDGGAMFGVVPKTMWQKLNPADENNMCTWAMR
                     CLLIEDNNRLMLIDTGMGDFIDDTYKKRYQPFGDDTLETSLNKHGFSPDDITDVFLTH
                     LHFDHCIGALKDDGNGKPTLRFPNATHWSSFAQWKSAMQPNAREKASYVHNYLMAIQE
                     SGKLKFIENDDLGMRNVEIEYVYGHTESMMLCHIYKDKQIISYCADLIPSHHHIKLPY
                     IMAYDIRPMESLQEKTIFLEKAYENKHILFFEHDKEVECCTLKRTEKGIEFDKLMKLE
                     ELV"
     gene            complement(4501..4965)
                     /locus_tag="PLL59_10580"
     CDS             complement(4501..4965)
                     /locus_tag="PLL59_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMY24294.1"
                     /translation="MQSTAKTADDYLNTLDENRKSAMMTLRNVIVKNLPKGFEETMQY
                     GMISYVVPHSIYPDGYHCQPKQALPFLSIAAQKNFIAVYHMGIYADAQLYQWFVTEYA
                     KHCTAKLDMGKSCIRFKNGNQIPFELIGELAKKQSVKDWINLYELNYKKKTK"
     gene            complement(5083..5559)
                     /gene="ribH"
                     /locus_tag="PLL59_10585"
     CDS             complement(5083..5559)
                     /gene="ribH"
                     /locus_tag="PLL59_10585"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMY24295.1"
                     /translation="MASALQNLSVYDTEKVPNGKGKSYGIAVAEWNKHITFELLKASI
                     ETLLQHGVEDNDIYVSYVPGTFELPFAAKVLAETHHLTGVICLGCVIKGETDHDIYIN
                     TSVANALQQLNITFDIPFIFGVLTPNSEQQALDRAGGKHGNKGVEAAVTALKMSIL"
     gene            complement(5564..6268)
                     /locus_tag="PLL59_10590"
     CDS             complement(5564..6268)
                     /locus_tag="PLL59_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY24296.1"
                     /translation="MAKIKSTNTQKDILEKSDEQISQVFSDAEYFFDQYKNIIIGVIA
                     AVILGIGGWWAYNNLVKGPKESKAQDAIIYAQRYFDIDSMQLALKGDGKNIGFEAVAK
                     KYSGTSAGNRAAFGAGVASLKLGKYADAVKYLEDFSTDDPILSARKYGCLGDAYAEQN
                     KMDNAIENYKKAVAAADNETTAPTYLYRAALAIEQKGNKKDALELYKELNTKYPNSQE
                     GMAAEITIAKLEAQAL"
     gene            6383..7480
                     /gene="recF"
                     /locus_tag="PLL59_10595"
     CDS             6383..7480
                     /gene="recF"
                     /locus_tag="PLL59_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HMY24297.1"
                     /translation="MPICTFYILQLKLSNYKSYSFKEFNFSEKLNFIVGNNGIGKTNL
                     LDAVYYSCLSKSYFSSDLMVPNFQNVFFRIESNISLGIDNQLVEFKYLKQEKKEISLN
                     KVKVEKLTSFVGKYPCVFITPDDNQLILGASEIRRKFIDATLCQFSSDYLLHLVSYNK
                     VLAQRNALLKSFYEKRTFDNALLDVYNQQMVQAGNFIHQFRKEFMLKFVPVFEKYYRQ
                     IFDGDEQIDIHYHSDLLDNDYATLLQTTQEQDRVAQRTTKGIHLDDIVFEMKNFPLKK
                     IASQGQQKTVLLSMKLAQFELLKIEKQVMPLLLLDDIFDKLDSGRITKIFQLIKSESF
                     GQVFISDTNIDKIQQLIENNNCKQYKIIPLG"
     gene            7506..7772
                     /locus_tag="PLL59_10600"
     CDS             7506..7772
                     /locus_tag="PLL59_10600"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HMY24298.1"
                     /translation="MKDVLDDMIKEMRIGGKMEEMNVRKYWHELMGAYITNQTSKIFY
                     KQGKLFVYLESAALKQELFMAREKIISRLNERLQENLIQEIIFR"
BASE COUNT         2502 a         1296 c         1325 g         2718 t
ORIGIN      
        1 agaaacgaag ataaatatat aacaatcatt aaaaaaaata gatttccctt tattcaaaat
       61 gctgtttttt ccgaattaaa atcaaatttc tatgtatatg ttgatggttt gattctaacg
      121 gcttctaata gcggagaaat acaaatcaga ccatttttat cgaattatta taagcaagtt
      181 atttggaatg catttttgtc tattctatat ttttggtcca atgacaaaag taatttcaaa
      241 gaaaatacag atgtaatagt cgaaaaaact attcattttg cttttgattt attggcacca
      301 aatgcaatag attctggtat tgactatttg caacatttta ttaaacttag aaagtaattt
      361 tgtagtatga tggaagaaca agaaaaaatt ccaacatcta aagtggctag agcatcaagc
      421 gtgttccgta ccagcatgcg tgtaggtgcc aattatctaa aacacaatgc acaaaaaatg
      481 gtgcataaag aagttaatga agatgcctta aacgaaaaaa atgcatctga attgtttaac
      541 ctcatgagtc aattaaaagg cagtgccttg aaaattgcgc aaatgctaag catggataat
      601 ggcttattgc caaaaggtta tgccgaaaaa tttgcattag cacaaaatag cgcaatggca
      661 ttaagtagtc cattagtaat gaacactttt atgaagtata tgaatcagtc gccttatgat
      721 ttatatgatt catttgaaat aaaagcaaat catgccgcat caatagggca ggtgcatttg
      781 gcttataaaa atggtaataa actggctgtt aaactgcaat atccaggcgt tgccgatagc
      841 attcacgcag atatccaaat ggtaaaacct ctgttattgc gcatagtagg cataaaagaa
      901 attgaagtaa aacaatattt tgacgaattt gaagagcgac taattgatga gtgtaattat
      961 caattagaat tgagtaatgg tgttgaaata gctgccgcta ctaagcatat tcccaactta
     1021 attattccga cttattatcc agctttatct tcatcgaaag tgctgaccat ggaatggatt
     1081 gatgctcaac ctatgcaggt ttttctagat acggaaacga atcaagcgaa caaagataaa
     1141 ataggacaag cattgttgga ttttttgcat acctctattc acgatttgaa aaaatttcat
     1201 gcagatccac atccaggcaa ttttttagtt acaaaagata tgcaattagt ggtgcttgat
     1261 tttggttgtg tgaaatcaat tccaaacgat ttttataact attatttctc attggttcgc
     1321 aatgatgtgc ttgctgatac cgataaatta cgcatttgcc ttaaaggctt agatatgttg
     1381 cgcgattcgg acacagcggc cactgatcag ctttttatgg aaacgtcttt aaaagccatt
     1441 caatgtttgt caaagccaat gaaatcagat actttctact tcggtgatct tgcgtattac
     1501 gaacaattgc aattgcaagg cgaagaattt atgaataata aagcacttag aacgccagaa
     1561 gccatgcgtg gatctcgtca tgctatttat ctgcatcgta cattttttgg tttgtattcc
     1621 atcttgcaca aactaaatgc aaccgtgcgt attgatagaa gatttattga aaatgttggt
     1681 gtttagttta aaaactatgc taactttata gtatagttta gatttccgtg aattaaaaaa
     1741 cattgttatg aaaaaactag atgcatcaat cgtaatatac aatagtatgt tatctgattc
     1801 agaacaagaa gtggctaacg ttttagcaaa taagataaat caattattac cgaacgcaaa
     1861 acagaaaata tggcatagtc atcctgtttg gtttattaat gacaatccaa tcgtaggtta
     1921 tagtaagcaa aaattaggca ttcgcttaat gttttggagt ggcgctgatt ttgtgcaaca
     1981 taaatttacc gtgataggca agaagtttaa agatgcttct atattttata aaacactaag
     2041 cgaaatcaac tattctgatt tagaaaactg gattttacaa gcaaaagcaa tacaatggga
     2101 ttataaaaat atcgtaaaac gaaaaggctt attgcttcgt ttgttgtaat tattttgctg
     2161 tcaaattaat agatttattt attggttttt tattgcttgt tgtttttaca attagttgtt
     2221 cttgtgtgta tttatcttta gtacaagtga atgtatattt tgtattcggc tgtaagatga
     2281 atgctatctt tttgttgact tcaatagttt taatgagact atcattttct tgtacactta
     2341 gctgtacggc atctgctaat ttgctgtcgc aatatacatt taattcaaag gcgatatctt
     2401 taaattgaaa actgtaaatg tcatcgctgc ccattccacc gcttctgtca gatgagaaaa
     2461 aaccattcga ataattggca tctatataaa aaccaaaatc atcggttgca ctattgaacg
     2521 gctttcctaa gttttctggc tttccaaatt cacccaattt atttggcaca catttaaaaa
     2581 tatccatacc accaaaacct ggcaaacctg tagaggaaaa atataaagtg ccatcatgat
     2641 gaataaacgg ataacgttcg tcgcctttcg tgtttacttc ctttcctgcg tttttaggct
     2701 tgctccattt ctcattttga taagtggaat aataaatatc tttaccacca aatccgccac
     2761 ctctgtcgga agtaaaaaat aacatattgc cttctgtatt aatactcgga aatgcacatg
     2821 aatattcttt gtcatttaac tccaattctt tgatatcctt ccagccatct ttactttttt
     2881 tggcagaaaa aagttttaaa gtaacatctc cttttttatt ggtcacggca tcgccatatt
     2941 gaaaattgtt ttttgtgaaa tagatgatat cgtgtaatgt atccacgcat gctggtccgc
     3001 tgttgtagtt tttggtattt acaacacctt tcactggctc gactgtcatt gtagagtcag
     3061 cattgtaaaa tgcataatat acttcttgaa aaccactgcc agaagtgcca tttattttgc
     3121 cttgacgagc agaggtaaat aggatgccat tgtcatatgg aacggcgcaa aaatctgcat
     3181 gtatagaatt gagtgctttc atattttgca cttctatttt gcgagctgaa gttggattgt
     3241 ttttttgttg ctcacacgac aataatagtg ttctggcaat gcttgtgtcg gcatcgtttt
     3301 tcaatgttaa atatttttgt aaccatattt tggcatcttc atatttttca tttgccaata
     3361 gcatttgtcc ataatataaa tatgatttcg gaatggcatt tttatcagca gtcaccaagg
     3421 catagtattt ttctgcttgt tggtaatcat taatttttct atagcattca gctattttgc
     3481 gaattgcttt aatatttcct ttttcagcga taggtttata taattcgatt gcttgtgcgt
     3541 atttataatc agcatataat ttatcagctt tatccatttg cgcataacta gaaatacaaa
     3601 ataatagtaa agcgatgaaa agcgtaatat ttcgatactt gaacacttat acgtataatt
     3661 gggtttaaac taattcttct aatttcatta atttatcaaa ttctatgcct ttttctgtgc
     3721 gttttagtgt gcagcattct acctctttgt cgtgttcaaa aaataggata tgcttatttt
     3781 cgtaggcctt ttctaagaag attgtttttt cttgcaaaga ttccatcggt ctaatgtcat
     3841 acgccatgat atacggtaat ttgatatgat gatgagatgg aattaagtcg gcacaataag
     3901 aaattatctg tttgtcctta tagatgtggc atagcatcat agattctgta tgtccataaa
     3961 cgtattctat ttctacatta cgcataccta agtcgtcatt ttctataaat tttaattttc
     4021 ccgattcctg aattgccatt aaataattat gcacgtatga cgctttttct ctagcatttg
     4081 gttgcatagc tgatttccat tgtgcaaatg aggaccaatg tgtagcatta ggaaagcgta
     4141 atgttggctt tccatttcca tcatctttta atgcacctat acaatggtca aaatgtaaat
     4201 gcgttaaaaa aacatctgtt atgtcgtcag gcgagaagcc atgcttgttg agtgatgttt
     4261 ccaaagtgtc atcaccaaaa ggctggtatc tttttttgta tgtatcatcg ataaagtcgc
     4321 ccatgccagt atcaataagc atcagccgat tattatcttc aattaataag caacgcatag
     4381 cccaagtgca catattattt tcatctgctg gattgagttt ttgccacatt gtttttggca
     4441 caacgccaaa catggcgcca ccatcgagtt taaaaaagcc agtgtctatt gtgtaaagtt
     4501 tcattttgtt tttttcttat aatttaattc atataaatta atccaatctt taacgctctg
     4561 tttttttgcc aattcgccga ttagctcgaa tggtatttga tttccatttt taaaacgtat
     4621 gcaagatttt cccatgtcga gtttggcagt acaatgttta gcatattccg ttacaaacca
     4681 ttgataaagc tgagcatctg catatattcc catgtgataa acggctataa aatttttctg
     4741 tgcagcaatt gaaagaaacg gcaatgcttg tttgggttgg caatggtaac catctggata
     4801 gatgctatgt ggcactacgt aactaatcat tccatattgc atagtttctt caaaaccttt
     4861 aggaagattt tttacaatta catttcgcaa agtcatcatc gctgatttcc tattttcgtc
     4921 taatgtattg agatagtcgt ctgccgtttt tgctgtagat tgcatagttt attttactac
     4981 aaaatacata aaatttagaa gctattattc gcaatcagta aaaatgatgt tggaataatt
     5041 ctgtataaca actaaaagca aaccattaag gttatatgga aattacagaa tactcatctt
     5101 caatgccgtt acggcagctt ctacaccttt attgccatgt ttaccgccag ctctatccaa
     5161 tgcttgttgt tcgctatttg gtgtaagcac gccaaagata aatggtatat caaatgtgat
     5221 atttagttgc tgtaatgcat ttgcaacaga tgtattgata tagatatcgt ggtcagtttc
     5281 gccttttata acacagccta aacaaattac gccagttaga tgatgtgtct ctgctaatac
     5341 ttttgcggca aaaggcaatt cgaatgttcc tggcacatag gaaacataga tgtcattgtc
     5401 ttcaacacca tgttgcaaaa gtgtttcgat gctagctttc agcaattcaa aagtgatgtg
     5461 tttattccat tcagcaacag caataccata tgactttccc ttgccattcg gtactttttc
     5521 agtatcgtaa acagataaat tttggagtgc agatgccatt gtgttataat gcttgagctt
     5581 ctaatttagc aatggtaatt tctgctgcca taccttcttg cgaattagga tattttgtgt
     5641 tcaactcttt ataaagttct aaagcatctt ttttattacc tttttgctcg attgctaatg
     5701 cagcacgata caaataagta ggcgcagttg tttcattatc tgcagcagca actgcttttt
     5761 tataattttc gatggcattg tccattttat tttgctcagc ataagcatca cctaaacaac
     5821 catattttct tgcagataaa attggatcgt cggtactgaa gtcttctaag tattttactg
     5881 cgtcagcata tttacctaat tttagactgg ccacaccagc gccaaaagca gcgcgattgc
     5941 cagcacttgt gccactgtat ttttttgcta cagcttcaaa tcctatgttt ttaccatcac
     6001 cttttagtgc taattgcata gagtcaatat cgaaatatcg ttgcgcataa atgatagcat
     6061 cttgtgcctt tgattcttta ggacctttta ctaaattatt ataagcccac cagccaccaa
     6121 taccaagtat tactgcagca ataacaccta taataatatt tttatattga tcaaaaaagt
     6181 attcagcatc gctgaaaact tgagagattt gttcgtcact tttttctaga atatcctttt
     6241 gtgtatttgt cgatttgatc tttgccataa ttatattatt taagcgtgcg caaaggtacg
     6301 ttttttaagc gatttttaaa aatatttgtc cactatatcc acatttttta cgattacgct
     6361 ataatgcgct tatctttgta gcatgcctat ttgcaccttt tatattctac aattgaaatt
     6421 aagcaattat aaaagctata gctttaagga gttcaatttt tcagaaaaat tgaattttat
     6481 agtcggaaat aatggcatag gaaaaaccaa tctcttagat gctgtatatt attcttgtct
     6541 gagtaaaagc tatttttcta gtgatttaat ggtgccaaat tttcaaaatg tattttttag
     6601 aattgaatct aatatttcat taggcataga taatcaatta gttgagttta agtatttaaa
     6661 gcaagagaaa aaagaaattt ctttaaataa agtaaaagta gaaaagttga cgagttttgt
     6721 tggaaagtat ccttgtgtgt ttattacgcc agatgataac caactgattc ttggtgccag
     6781 cgaaatacgt cggaaattta tagatgctac attgtgccag ttttcttctg actatcttct
     6841 tcatttagtg agctacaata aagtgctggc gcagcgcaat gccttgttga aatcttttta
     6901 tgagaaaaga acgtttgaca atgctttgct tgatgtgtat aatcaacaaa tggtacaagc
     6961 tggcaatttt atacatcaat ttcgaaagga atttatgttg aaatttgtgc cggtatttga
     7021 aaaatattat agacaaatat ttgacggcga tgaacaaatc gacattcatt atcattcaga
     7081 tttactagat aatgactatg cgacattatt gcaaacaaca caagaacaag accgtgttgc
     7141 tcaacgcaca acgaaaggta ttcatttaga cgatatcgta ttcgaaatga aaaattttcc
     7201 gctcaaaaaa attgcctcgc aaggtcagca aaagacagta cttctctcta tgaagctggc
     7261 tcaatttgaa ttgttaaaaa tcgaaaaaca agtaatgcct ttgttgttac tcgatgatat
     7321 ttttgataaa ttagacagcg gaagaattac aaaaatattc cagctgataa aatcagagtc
     7381 tttcggacag gtatttattt cagatacgaa tatcgataag atacaacaac taattgaaaa
     7441 taataattgc aaacaatata aaattatacc acttggctaa ttataggaat agcagatttg
     7501 cgtccatgaa agacgttctc gacgatatga taaaagaaat gcgtatcggt ggaaaaatgg
     7561 aagagatgaa tgtcagaaaa tattggcacg aattaatggg cgcatatatc accaaccaga
     7621 cttcaaaaat attttataag caaggcaaat tgtttgtgta tttggaatca gcagccttga
     7681 aacaagaact ttttatggct cgcgaaaaaa taataagcag actaaatgag cgcttgcaag
     7741 aaaatttaat tcaggaaatt attttcagat agaatttttt tatgagtaca tcaaaatttc
     7801 ttatttttaa aagaagtaca ttttttatac ttttgaatcc t
//
LOCUS       DAOQQV010000120         7685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3785_length_7685_cov_8.085321, whole genome shotgun sequence.
ACCESSION   DAOQQV010000120 DAOQQV010000000
VERSION     DAOQQV010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3785_length_7685_cov_8.085321"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLL59_10605"
     CDS             complement(<1..1137)
                     /locus_tag="PLL59_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24299.1"
                     /translation="MRNYIIFFWLCVLSFSLKAQYPKEFPADNASYGKAYAEFIKANC
                     TREDCKEIAEKFPKAITTGKASVYFFKIKGITQSMLLKKAPAYPAFKELADLLLALET
                     TKASGTAIDKNFDILQALIDRSKSGNIKEFVGYIEYLTNLYSKNALYYTNTNIWQTTG
                     DYTVEFTNEKPVFIFAATDLIGMSNADTLTIKNEKGKFFPLENLWIGNNGTIGMQRAG
                     FDPANNFVNFSHHQINFAKSDIVIDSAKFTFKPVLNEVLLGTYTDKLLQANSQERFYP
                     KFRSYSNNVNFNTLSKEVKMSAGFQLEGTKIYGVGSDTVLPKITLLAKDGKELVRVTA
                     NRIQLKDFKDINAEDASFSIFLADNTISHPFVNFSYFSKSKDVKV"
     gene            complement(1156..1980)
                     /gene="prmA"
                     /locus_tag="PLL59_10610"
     CDS             complement(1156..1980)
                     /gene="prmA"
                     /locus_tag="PLL59_10610"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HMY24300.1"
                     /translation="MIRVYTISYPDSIENEEFAGLFFEVESEGVEEKETCVEVYLSEQ
                     QQAAGEQIIQAICDTYQVDFTQSILENKNWNAQWESSFQPILIEDFVFVRTTFHEPNQ
                     NVAHEIIIEPKMSFGTGHHPTTSQMMQNMRLIDFKEKSVLDCGSGTGILAILAAKLGA
                     TYCLALDNDEWCYQNCIENIELNKVENITPIIGEIAQINQQKFDVILANIHRNFLTAY
                     MSDLSLLLKDDGYVIVSGFYEEDAKIILDNAAMHHLIANYHTTQNNWTCIVLQKKN"
     gene            complement(1977..2747)
                     /gene="tpiA"
                     /locus_tag="PLL59_10615"
     CDS             complement(1977..2747)
                     /gene="tpiA"
                     /locus_tag="PLL59_10615"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMY24301.1"
                     /translation="MRKKIIAGNWKMNAVKEEAIALLNGIVEQYNTYNLSPNKSVIIA
                     TPFPYIDYCATQFSNFQFLHAAAQNCSEYEQGAYTGEVSAKIIASLGVQYVIIGHSER
                     RQYFNENDFVLLAKIKQAQLQNMIPIFCCGEPIEVRENEEHFSFIKQQLSHCIFHLTP
                     QEIKNCIIAYEPIWAIGTGKTATQAQAQEMHAFIRKEIENKFDKPIADSISILYGGSV
                     NAVNAAELFASNDIDGALVGGASLKINDFSTIINAMPS"
     gene            complement(2777..3946)
                     /locus_tag="PLL59_10620"
     CDS             complement(2777..3946)
                     /locus_tag="PLL59_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HMY24302.1"
                     /translation="MPKHSFVLFDGNERDNLLPLTATRAVAAMRVGILTIKEKWEKYL
                     NTKVGIATQAYLQDKYGTINSSNAVIYINACILPNPQLVEAILQLSDNSILKNGNQQI
                     AFSTTQSLSDLSAIHAYSSNYESTFTNYEFVEFPWSIFTLNGNEIRNDIQLLELKPNP
                     EKLSKTNTILGHEIYVEDGVQCECAILNAKDGPIYLGKDSEIMEACTIRAPFALGEHA
                     CLKMQTKIYGDTTVGPYCKVGGEVSNSVFFAYSNKGHDGFIGNSVIGEWCNLGADTNN
                     SNLKNNYGKVKAYNYPANDFINTGLQFCGLIMGDHSKSSINTMFNTGTVVGVCTNVFG
                     GGFHPKFIPDFAWNEKIFELEKANEVAQRMMERRKINFSETDKAIFKYIFENINK"
     gene            complement(4064..4312)
                     /locus_tag="PLL59_10625"
     CDS             complement(4064..4312)
                     /locus_tag="PLL59_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HMY24303.1"
                     /translation="MKADIHPKEYRFVVFKDFSCDHMFLGKSCANTRDTIKWEDGNEY
                     PLVKLEVSNMSHPYYTGKQKLLDTAGRIDKFNKKYAKK"
     gene            complement(4454..5539)
                     /locus_tag="PLL59_10630"
     CDS             complement(4454..5539)
                     /locus_tag="PLL59_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24304.1"
                     /translation="MPNISKSQDVDGVWTGNFIQYIHNYYDVELKVQKLAPGDIFSAR
                     LRIMDSYYFGEYNVSGYICNKKYLEITTIVLLRESGPSNWIDCLNGTFDLNDDETLLT
                     FTDTWLQQNSKNNACKVKYIQKDMFQCLRSAYLHKAKYLSEITAFEQKWNDVTLARSL
                     NKKALEEMLANTKPSIKSTKKDSTLTTPTNINVAPDTALLTNQFIAASKTEQQVNIAS
                     KLEDFNKTETKIVAKNEDRAPVIDTYEKVQTRDVVVKDEIEVSSPEITIEYWDRYNED
                     GDSVNLFMNKKPILTNVLLTKAKKTIVIKLEKEKNYLVLDAINLGKEPPNTASITVKD
                     GKKIQNVSLTSTMQTSGALKITYKKTP"
     gene            complement(5664..6269)
                     /gene="yihA"
                     /locus_tag="PLL59_10635"
     CDS             complement(5664..6269)
                     /gene="yihA"
                     /locus_tag="PLL59_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HMY24305.1"
                     /translation="MIQSAKFIGAFPKTDDCPTTNLSEFAFIGRSNVGKSSLINLLTN
                     RKDLAKVSKTPGKTQTINFFLIDESWYLVDLPGYGYARVSKSTREVFSKMIKYYISHR
                     EQLLNLFVLIDATITPQKIDLDFINWLGEMRIPFSIIFTKTDRDKTLAIQKNVKLFKQ
                     KMLESWTALPPMFLTSSEKNRGGKEVIDFIKNSMSEEETDI"
     gene            6389..7105
                     /gene="ubiE"
                     /locus_tag="PLL59_10640"
     CDS             6389..7105
                     /gene="ubiE"
                     /locus_tag="PLL59_10640"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HMY24306.1"
                     /translation="MGTPLTPYNSGESKKEQVATMFNNVAGTYDFLNHFFSVGIDKLW
                     RRKLVKLIGKTKPKRILDVATGTADLAIAETKLNPEQIIGIDISEKMLEIGKSKTQHF
                     PNIQLQIGDSENIQFAENTFDAVSVSFGVRNFENVNIGLKEMRRVLKPGGKVFILEFS
                     KPNNWLIQKLYYFYFCNVLPLIGKLVSKDARAYTYLPESVRIFPDGKKFVELLQYAGY
                     KNIECIPLTFGISTIYIAEK"
     gene            7159..>7685
                     /locus_tag="PLL59_10645"
     CDS             7159..>7685
                     /locus_tag="PLL59_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMY24307.1"
                     /translation="MENDLKFSRKRYHFGIHLGAGLGDFKIRHNALFGSSDSILTIKS
                     KYGYNFEIAALISYHINKYLEIRTVPGFAFSNKELNYGFADETKVIKKIDQIFFDLPV
                     ELKIKSEPLRDVKLYLIAGLKYGYDIGGNFKARRDEDMPKMSAHDFGVNYGVGMEIHF
                     PLFIFSPEFRVFNTVL"
BASE COUNT         2372 a         1327 c         1241 g         2745 t
ORIGIN      
        1 tacttttaca tcttttgatt tagaaaaata ggagaaattc acaaaaggat gcgagatagt
       61 attatctgct aagaaaatag aaaaagaagc atcttccgca ttgatatctt taaaatcttt
      121 tagttgtatt ctgttggccg tcacgcgaac taactctttg ccatctttag caagtaatgt
      181 aattttaggc aaaacagtat cagagccaac tccatatatt ttagtgcctt ctagctgaaa
      241 gcctgctgac attttcactt ctttgcttaa tgtattgaag ttgacattat tgctataaga
      301 acgaaatttt ggataaaatc gttcttgaga atttgcttgt aataacttat cagtataagt
      361 gcccaaaagc acttcattca atactggttt gaaggtaaac ttagcagaat caattacgat
      421 atctgatttt gcaaagttga tttggtgatg cgaaaaattc acgaaattat ttgcaggatc
      481 aaaaccagca cgttgcatac cgattgttcc attatttcca atccataaat tttccaatgg
      541 aaagaatttt cctttttcat tttttatggt caatgtatca gcattcgaca tgccgattaa
      601 atcagtagca gcgaagataa atacaggttt ttcgttggtg aattcaaccg tataatcgcc
      661 tgtagtttgc catatattag tattggtata atacagtgca tttttcgaat aaaggtttgt
      721 taggtattca atgtagccaa caaactcttt aatatttcct gattttgatc tatctattaa
      781 agcttgtaga atatcaaaat ttttgtcgat tgctgttcca cttgcctttg tagtttctaa
      841 tgccaataat aaatctgcta actctttaaa agctggatag gctggtgctt tctttagcaa
      901 catcgattgt gtaatgcctt taattttgaa aaaataaacc gatgcttttc ctgttgtgat
      961 tgcttttgga aatttttcgg caatttcttt acaatcttca cgtgtgcaat ttgcttttat
     1021 aaattcagca tacgcttttc cgtaactagc attatctgcc ggaaactcct ttggatactg
     1081 tgctttcaaa gaaaaagaca agacacaaag ccaaaaaaaa atgatataat ttctcattcg
     1141 taatttttaa agtttttagt ttttcttctg taatacaata caagtccaat tattttgtgt
     1201 cgtgtgataa ttagctatta aatgatgcat tgctgcatta tctaaaataa ttttcgcgtc
     1261 ttcttcataa aaaccagaca caatgacata gccatcatct ttcaacaata aggataaatc
     1321 agacatatat gctgtcaaaa aattgcgatg tatatttgct aatatgacat caaacttttg
     1381 ttgatttatc tgagcaattt cgccgataat tggcgttata ttttctacct tgtttaactc
     1441 tatattttct atacaatttt gatagcacca ttcatcgtta tcgagtgcca ggcaatatgt
     1501 agcacctaat tttgcggcaa gaattgccaa tatacctgtc cctgagccac aatccaaaac
     1561 tgacttttct ttaaaatcaa tcaagcgcat gttttgcatc atttgcgatg tggtaggatg
     1621 atgtcctgtg ccgaacgaca ttttaggctc gataattatt tcgtgcgcta cattttgatt
     1681 aggttcatga aatgtcgtgc gcacaaatac aaaatcttca atcaaaattg gctgaaaaga
     1741 gctttcccat tgtgcattcc aatttttatt ttccaatatt gattgtgtaa aatcaacttg
     1801 ataggtatcg caaatagctt ggataatctg ctctcctgct gcttgttgct gctcgcttaa
     1861 atacacttcg acacaagttt ctttttcctc tacaccttcg ctttctactt caaaaaacaa
     1921 tcctgcaaac tcttcgtttt cgattgaatc agggtaagat attgtatata cgcgaatcat
     1981 gatggcattg catttatgat ggtggaaaaa tcatttatct tcaaggaagc accgcctacg
     2041 agtgcgccat ctatatcgtt gctagcaaat aattcagcag catttacagc atttacgctg
     2101 cctccataaa gtatagaaat gctatctgca atcggtttat cgaatttatt ttctatttct
     2161 tttctaataa atgcatgcat ttcttgtgct tgcgcttgtg tagctgtttt gcctgtgcct
     2221 attgcccaaa tcggttcata agcaatgatg caatttttaa tttcttgtgg tgtaagatga
     2281 aaaatacaat gcgataattg ttgcttgata aatgaaaaat gttcttcatt ttcgcgcact
     2341 tctattggct cgccacaaca aaatattggt atcatatttt gtagctgtgc ttgtttaatc
     2401 tttgctaata aaacaaaatc attttcatta aaatattgac gtcgttctga gtgaccaatg
     2461 atgacatatt gcacgccaag ggaagcaatt atttttgctg aaacctcgcc tgtgtatgca
     2521 ccttgctcat attcactaca gttttgcgct gctgcatgta aaaactgaaa gttggaaaac
     2581 tgtgttgcac aatagtcaat atatggaaat ggcgtcgcta taatgaccga tttatttggc
     2641 gataaattat aagtattata ttgttctaca atgccattta gcaatgctat cgcttcttct
     2701 ttaacagcat tcattttcca atttccggct attatttttt ttctcatggt cgtaatttat
     2761 agcacgcagt gtgcctttat ttattgatat tttcgaagat atatttaaaa attgccttgt
     2821 ctgtttctga aaaatttatt tttctgcgct ccatcatgcg ctgtgccact tcattcgctt
     2881 tttctaattc aaatatcttt tcattccaag caaaatcagg tataaattta gggtggaatc
     2941 cgccaccaaa tacattagta caaacaccaa caactgtgcc tgtattaaac atcgtgttga
     3001 tgctactttt ggaatgatcg cccataatta gaccacaaaa ttgtaagcct gtgtttataa
     3061 aatcattcgc tggataatta tacgctttta cttttccata attgttttta agatttgagt
     3121 tattggtgtc tgcacctaaa ttacaccatt cgccgattac cgaattgcca ataaagccat
     3181 cgtgtccttt gtttgaataa gcgaaaaata ccgaattcga aacttcgcca ccaactttgc
     3241 aatatggccc aactgttgtg tcaccataaa tttttgtttg catcttcaag catgcatgct
     3301 ctcctaaagc aaatggcgca cgaatggtgc aagcttccat gatttcgctg tctttaccta
     3361 aatagatagg tccatctttt gcatttaata tggcacattc gcattgcaca ccatcttcga
     3421 cataaatttc atgtccaaga attgtatttg tttttgataa tttttcagga tttggtttta
     3481 attctaagag ctgaatatca ttccgaattt cattgccgtt aagcgtaaaa attgaccatg
     3541 gaaattcgac aaattcgtaa ttagtaaatg tgctctcata atttgatgaa tatgcatgaa
     3601 tggctgataa atcgcttaaa ctttgtgttg tagaaaatgc gatttgttga ttgccatttt
     3661 tcagaatgct attatcggat aactgcaaaa ttgcctccac taactgtggg tttggcaaaa
     3721 tgcaagcgtt gatgtatatc acagcatttg atgaatttat ggttccatat ttatcttgta
     3781 aatacgcttg tgttgcaatt ccaactttcg tgtttaaata tttttcccat ttttctttga
     3841 tggtcaaaat accaacgcgc atagcagcaa ccgctcgagt agcagttagt ggtaataaat
     3901 tgtcgcgttc atttccgtcg aagagtacaa atgaatgttt cggcatagtg taaaaataga
     3961 atttatacac ttataatcat agtgaaaaaa cactaattcc ttagtttagg cataaaaaaa
     4021 acgctctgaa atcagagcgt ttgtatagct ataataatat gaattatttt tttgcgtatt
     4081 ttttgttgaa tttatcgata cgaccagctg tatctaataa tttttgctta ccagtatagt
     4141 aagggtgaga catattagaa acctctaatt tcactaatgg atattcgtta ccgtcttccc
     4201 atttaatggt atctctagta tttgcacaag atttgcctaa aaacatgtga tcgcaagaaa
     4261 agtctttaaa cacaacaaat ctgtattctt ttggatggat atctgccttc attttagtat
     4321 aatttgaggt gcaaagataa tactttattt gtaattctaa aaatgcagtc ttcattttta
     4381 aaaaatgata acattacact aaaacagctt atttaaaagt acaaatattt gattataaat
     4441 acattacaat caactaaggt gtcttcttat aggtaatttt caatgctcca ctagtttgca
     4501 tcgtagaagt taaggataca ttttggattt ttttaccatc ttttaccgtt atagatgcag
     4561 tattaggtgg ttcttttcct aagttgatgg catctaaaac caagtaattt ttttcctttt
     4621 ctaatttaat gacaatggtc ttttttgcct ttgtcaaaag cacattcgta agtattggct
     4681 ttttgttcat aaacaaatta acagaatcgc catcttcatt ataacgatcc caatattcga
     4741 tagtaatttc aggactactt acttcaattt catctttcac aacgacgtct ctggtttgta
     4801 ctttttcata cgtatcgatt actggtgctc tatcttcgtt tttggcaacg attttcgttt
     4861 ccgttttatt aaaatcttct aatttagaag caatattcac ttgttgttcc gttttagatg
     4921 ccgcaataaa ctgattagtt aacaaggctg tatctggtgc tacattaatg tttgttggcg
     4981 ttgtaagtgt gctatctttt tttgttgatt taatggaagg ttttgtattt gccaacattt
     5041 cttccaatgc ttttttgttt aagcttcttg ccaaggtaac gtcgttccat ttttgttcaa
     5101 atgctgtaat ttctgataaa tattttgctt tatgtaaata agcagagcgc aagcattgaa
     5161 acatgtcttt ctgtatatat tttaccttac atgcattgtt tttactattc tgttgcaacc
     5221 aagtgtctgt gaaagtcaac aaagtttcat catcatttaa atcgaaagtg ccgtttagac
     5281 aatcaatcca attggaagga ccactttcac gcaacaatac aatggttgtt atttctagat
     5341 actttttatt gcagatataa ccgctcacat tatattctcc aaaataatag ctatccatta
     5401 tgcgcaatct ggcggaaaat atatcacctg gtgctaattt ctgcactttc aattcaacgt
     5461 cataataatt atgtatatat tgaataaaat ttccagtcca aacgccgtca acatcttggc
     5521 tcttggaaat atttggcatt aataatagta tgcaacacca taagaaataa ctacgtaaac
     5581 ttttcacaat tacaaaatta cttgaatact agtgtattca tcaaatatat tgccaaacat
     5641 gtgttgataa ttatacgaca acattaaatg tctgtttctt cttcagacat actatttttt
     5701 ataaaatcta ttacttcttt gccgcctcta tttttttctg aagaagtgag gaacatgggt
     5761 ggcaatgctg tccagctttc caacattttt tgtttaaata attttacatt tttttgaata
     5821 gcgagcgttt tatctctatc tgtttttgta aaaataatag aaaaaggaat gcgcatttca
     5881 cctaaccaat tgataaaatc taaatctatt ttttgtggtg taatcgtagc atctattaat
     5941 acaaataagt ttaacaattg ctcacgatgg gaaatatagt attttatcat tttactaaac
     6001 acttcgcgcg tgctttttga tacacgtgca tagccatagc caggcaaatc tactaaatac
     6061 caactttcgt caatcaagaa aaaatttatc gtttgtgttt ttccaggtgt tttcgacact
     6121 tttgccaaat cttttctgtt ggtgagcaaa tttattaaag aagatttgcc gacattgctt
     6181 cttccgatga atgcaaattc cgacaagttt gtcgtcggac aatcgtctgt ttttggaaat
     6241 gcgcctataa attttgctga ctgtatcaaa ttgaagatta attaatgcaa agatgaggac
     6301 attttttgag agattggtat ttaatttcta cttttgtgta gaatagttgt tcagaaagga
     6361 atttcttttg aatacttact ataaaaaaat gggaacaccg ttaacaccat ataattcagg
     6421 cgaaagcaaa aaagaacaag tagctacaat gtttaataat gtagctggta cttatgattt
     6481 tctgaatcac tttttttcag taggcataga taagctatgg cgcagaaaat tagtaaagct
     6541 catcggaaaa acaaaaccta agcgcatctt agatgttgca actggcactg ctgacttagc
     6601 cattgcagaa acaaaactaa atccagagca aataattgga attgatattt ctgaaaaaat
     6661 gctggaaatc ggcaaaagca aaacgcagca tttcccaaac atccaattac aaattggcga
     6721 tagtgagaat atacagtttg cagagaatac gtttgatgca gtaagtgtat catttggcgt
     6781 tcgcaatttt gagaatgtca atatcggatt aaaagaaatg cgtagagtat tgaagcctgg
     6841 cggaaaagtt tttattctcg aattttccaa accaaataat tggcttattc aaaaactata
     6901 ctatttttac ttttgtaacg ttttaccgct tattggcaaa ttagtttcta aagatgcacg
     6961 tgcttacact tatttaccag aaagtgtgcg tatttttccc gatggaaaaa aatttgtaga
     7021 gctattacaa tacgctggat ataaaaatat agaatgcata ccactcactt ttggcataag
     7081 caccatatac attgcagaaa aataatactt atcgtattag tacttctacc aatattcacc
     7141 tttgcacagc gcaatgtgat ggaaaacgac ttaaaatttt cgcgcaaacg ctaccacttt
     7201 ggcattcatc ttggcgcagg attaggcgat tttaaaattc gtcacaatgc cttatttggt
     7261 agtagtgata gtattttgac tatcaaatct aaatatggtt ataattttga aattgctgcg
     7321 cttatcagct accatatcaa taaatattta gaaatcagaa cagtgccggg atttgctttt
     7381 tcaaataaag aattgaatta tggctttgct gatgaaacaa aagtgatcaa aaaaatcgat
     7441 caaatatttt tcgatttacc tgtcgagtta aaaataaaat cagagccact gcgcgatgta
     7501 aaattatatc ttattgctgg cttaaaatat ggttatgata ttggtggcaa tttcaaagca
     7561 agacgcgatg aagacatgcc taaaatgtcg gcacatgatt ttggtgtaaa ctatggtgtc
     7621 ggtatggaaa ttcattttcc attgtttatt ttttcaccag aattcagagt gtttaatacc
     7681 gtact
//
LOCUS       DAOQQV010000121         7646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_3804_length_7646_cov_8.739033, whole genome shotgun sequence.
ACCESSION   DAOQQV010000121 DAOQQV010000000
VERSION     DAOQQV010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3804_length_7646_cov_8.739033"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLL59_10650"
     CDS             <1..255
                     /locus_tag="PLL59_10650"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HMY24308.1"
                     /translation="DKMNEHLPELKFFILPGGNKAAAVCHVARTVCRRAERGIVRLAE
                     QEEILPALVKYLNRLSDFLFVLARKMANDGNTPEIVWKSH"
     gene            361..582
                     /locus_tag="PLL59_10655"
     CDS             361..582
                     /locus_tag="PLL59_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HMY24309.1"
                     /translation="MYWTLELASYLEEAPWPASKDELIDFAIRIGAPLEVIENLQELE
                     DDEELYESIEDIWPEYPTKEDFLFNEDEY"
     gene            complement(629..1588)
                     /locus_tag="PLL59_10660"
     CDS             complement(629..1588)
                     /locus_tag="PLL59_10660"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HMY24310.1"
                     /translation="MKASKRCNKIEMKSVFYYAYCCCILFSTILMCAACQKVIHLDFD
                     NSVIKQVINANLYPNTNLTVNISKSKRADDYSAVEFLNDCKVDIYENDIFKETLAFVL
                     KDTLSGLGYYTSSFLLQENKTYRIVSTHATLGIAEASEYLPFKPGIINFALLQHADSL
                     QTKKDGHYIIVFQDSADFKEYYFLATYYRVLKPTIDNHGDTIYKYDYIWDIPSHTPLI
                     PNVNNFWPRSYTTDDNFNGQLTSLTVSFPSQYNTNYKEIALIIELSRLGKNFYEWNTQ
                     QLRYGIDYLNDGQYDRINIKSNIKNGFGHFSANSSTFIGIPIK"
     gene            complement(1585..4029)
                     /locus_tag="PLL59_10665"
     CDS             complement(1585..4029)
                     /locus_tag="PLL59_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY24311.1"
                     /translation="MKNYLYSSCMYVLLSLFGLVCYATNTYTFKGNILSQQTHKAIAF
                     ANIYDSTSRTFSTTDSLGKFEIRLAAGKYNFEFSCIGFNTIFREVNITKDSTIDISLQ
                     EIVQLDGVTVAAEKINKTATMSTSGVTTLSSMSVEKLPAFFGEKDIMKAILLTPGIQS
                     GQEGARGIFVRGGSPDQNLMLFHHATVFNASHIYGFLSVFTTESLKKMDIYKSYIPVQ
                     YGGRLSSVLNIEPNFGNTETWKGDYSISFITQKFHIEGPLKKEKTSFNFSIRDCHAGF
                     FTEPIAAKQYKRNGENGTLKYFFYDINAAIQHHVNDKNTLSWSLYTGSDFFTFGEGKE
                     YKRTTKYSKDYTQRKLSWQNIANSIEWKTNLKKQTIYNAYAYSFYNLSAKQQFAIVER
                     NYTNLSNDIHKTQYNNASKINEHKWLTMLEHAIKDMHHLNYGIKFNARTFHINTIDAK
                     VYDSTNNIIQSEKYVNPKVNTLDFYAFADYRFTWKNKLELNAGMQLFLYHVNKKSIFY
                     PQPRAELIYHPINGMSLRAAVSRTVQPMHLLTNNTGDILNDIWVPATSKIKPETAWQY
                     AGGIQYDHPKGYSASIDAYYKTMHHLSEYKYGTTFIVNNMAWDEQLLNSGTGKAYGLE
                     LFFAKTKGQFTAWLKYNLGWSKRQYPELNDGKTFYYKYDRRHDISIILQYKLKKHFDF
                     TMAWTYGTGWRMTTPNTTYASDETLYNYDRNNEPLTGSQDFMTVWNERNNYVLPAYHH
                     LDIGMNYTKQAKRVKHVLNISIYNVYNHMNIFTVFRDSEIDELGNKTRQYKQLSMFPI
                     IPSIGYTISFEIKKEK"
     gene            4352..5395
                     /gene="nirK"
                     /locus_tag="PLL59_10670"
     CDS             4352..5395
                     /gene="nirK"
                     /locus_tag="PLL59_10670"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HMY24312.1"
                     /translation="MRKWIIAPVLASLVMLGVSACKQNESAPATMSSAEAEKIVAEGN
                     YEEAEVTAPPLVPAPIGDRKAKKVIIRLETIEKVGELEDGTQYTFWTFNGTVPGSFIR
                     VRVGDEVELHLKNNENSTVSHNIDLHAVNGPGGGAEGTLVAPGKEAVVNFKVTNPGLY
                     VYHCATAPVPEHIANGMYGLILVEPAGGLPKVDKEFYIMQGDFYTSGKKGDKGLLSFN
                     LDKSIAENPDYVVFNGNATSLLGKNVLQAKTGETVRIYFGVGGPNLTSSFHVIGEIFD
                     KVYIEAGTKVNEHVQTTMVPAGGAVIVEFKATVPGDYVIVDHSLSRAFNKGAIGKIKV
                     TGDENPTVYKSKK"
     gene            5429..6259
                     /locus_tag="PLL59_10675"
     CDS             5429..6259
                     /locus_tag="PLL59_10675"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HMY24313.1"
                     /translation="MKSLPYLLILLLFIACETKQVNKQVLEDWKDEIKPVQSSKLMQK
                     IDSGTYQLFFGKDSGGLAIVPTFYLDETAVTNAEYLTFLKSNPQWTKSRVLHIFADST
                     YLSKWKSDYEIPANADPNAPVTNVSWFAAKAYAKSVGKRLPTVDEWEFAALASRDAKN
                     GKGTPEYDNYLMKSYLNRGMYLKPVKQSPANFYGIYDMFGVIWEWTEDFNSVMITNDS
                     RNNASDENLFCAGSSNSTSDLRNYAAFIRYAMRGSVKANYCINNYGFRCAKDVEASKK
                     "
     gene            6264..6833
                     /locus_tag="PLL59_10680"
     CDS             6264..6833
                     /locus_tag="PLL59_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMY24314.1"
                     /translation="MKRILILSLSILLFFACKNNTSNNDISADSVYHLPSQWENQAGQ
                     KMKMADLKGKVVVMVMIYSTCKTACPILTSEMGEIASRLADVSPKDIRFVMVSIDPQT
                     DTPERMTAYLKKNNFSGEQWLFLRSSEEETRELANVLSVKYKEISPVDFSHSNIISVF
                     SKNGTLAFQKEGLGIDVEGTVSEVRKQLQ"
     gene            6917..>7646
                     /locus_tag="PLL59_10685"
     CDS             6917..>7646
                     /locus_tag="PLL59_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HMY24315.1"
                     /translation="MNQLYAKVDSIKLNFDFRTKVEIDHGNHTLFPEGIKTKTHVSSR
                     ARIGFDGYFFSKLQLAIALQDARTWGESSTTGVKLLNNIVYEAWSAYWFTPKIAIKIG
                     RQKLAYDDERLIGGLDWAMQGRTFDALKGIFKLDSNANSQIELVATYNNDENTKNDTL
                     GRTVYGISEAGETTKSLQILHYSYKNAKKFQVSAIAVNNVLQNKSGKYYDMITIGMNV
                     KKYFKQVGIYGSAYYQGGKNTLGQK"
BASE COUNT         2508 a         1253 c         1384 g         2501 t
ORIGIN      
        1 gataaaatga atgagcattt accagaattg aaatttttta ttctgccagg tggcaataaa
       61 gcagctgccg tttgtcatgt agccagaaca gtttgccgac gtgccgaacg tggcatagtg
      121 cgccttgctg agcaggaaga aatattgcca gcattggtaa aatatctcaa tcgtttgtcg
      181 gattttttat ttgtattggc tcgcaaaatg gcaaacgatg gaaatacacc cgaaatagtg
      241 tggaaaagtc attgataatc agtgcaaaag aagtatgtta ttttttattt ggagatttaa
      301 attttaggca tatatttgca gtccttttaa tgattaacta acaaaataaa cgaataaaat
      361 atgtattgga cacttgaatt agcctcgtat ttggaagaag caccatggcc tgcgtcaaaa
      421 gatgagttga tagactttgc tattcgaatt ggtgcgcctt tagaagtgat tgaaaacttg
      481 caagagctcg aagacgatga ggaattatat gaaagcatag aagatatctg gccggaatat
      541 ccaacgaaag aagattttct gtttaatgaa gatgaatatt aaatcaaaaa aataaaataa
      601 aaatgtctaa ccttttgtta ggcatttttt attttatagg aatgccaatg aatgtgctgc
      661 tattggcact aaaatgacca aatccatttt taatattgct ctttatattt attcggtcat
      721 attgcccatc gtttaaataa tctatgccgt atcgcaattg ctgtgtgttc cattcataaa
      781 aatttttacc taacctagag agctcaataa tcaatgcaat ttctttgtaa ttagtattgt
      841 attgacttgg aaaactaacc gttaatgaag tgagctgtcc gttaaaatta tcatcagtcg
      901 tgtaggaacg tggccaaaaa ttattgacat taggaataag tggtgtgtgt gatggaatgt
      961 cccaaatata atcatacttg tatatcgtgt ctccgtgatt gtcaatagtt ggctttagca
     1021 cacgataata cgttgctaaa aaataatact ctttaaaatc ggcactatct tgaaatacaa
     1081 taatgtaatg tccatctttt tttgtttgca aactatctgc atgttgcaac aatgcgaagt
     1141 tgataatgcc aggtttaaaa ggtaaatatt cactagcctc tgcaatgcct aatgttgcat
     1201 gcgtagatac aattctatat gttttgtttt cttgtaataa aaaacttgaa gtgtaatagc
     1261 ctaagcctga taatgtatct tttaaaacaa atgctaatgt ctctttaaaa atgtcatttt
     1321 cataaatatc gactttacaa tcattcaaaa attcaacagc actataatca tcagcacgct
     1381 tgcttttgct aatatttacg gtcaagttcg tattaggata taaattggcg ttgatgactt
     1441 gtttgattac gctgttgtca aaatctaaat gtattacttt ctgacaagct gcgcacataa
     1501 gtattgttga aaataaaatg caacagcaat atgcataata aaacacactt ttcatttcta
     1561 ttttattgca tcgcttgctt gctttcattt ttcttttttt atttcaaagc taatcgtgta
     1621 tcctattgat ggaataatag gaaacatact gagttgttta tactgtctgg ttttgtttcc
     1681 caattcatct atttcgctat ctctaaaaac ggtaaaaata ttcatgtggt tatacacgtt
     1741 gtaaatgcta atattgagca catgtttaac gcgtttagct tgtttggtat aattcatgcc
     1801 aatgtctaaa tgatgatatg ctggcagtac ataattattt cgctcattcc atacggtcat
     1861 aaaatcttgt gagccagtta aaggctcatt gtttctgtcg taattatata aggtttcatc
     1921 gcttgcatac gttgtgttcg gcgtagtcat tcgccagcct gtgccatagg tccaagccat
     1981 agtaaagtca aagtgctttt taagtttata ttgcaatata atagaaatat catgccgtct
     2041 gtcatatttg taataaaaag ttttgccatc attgagttca ggatattgac gcttactcca
     2101 acctaaatta tatttcagcc atgctgtaaa ttgccctttt gttttagcaa aaaatagttc
     2161 aagaccatac gcctttccag tgccactatt caacaattgc tcgtcccacg ccatgttgtt
     2221 gacgataaat gttgtgccgt atttatattc tgatagatga tgcatagttt tataatatgc
     2281 atcaatgcta gcgctgtatc cttttggatg gtcatactgt atgccgccag catactgcca
     2341 agctgtttct ggttttattt ttgatgtagc aggcacccaa atatcattga gtatatcgcc
     2401 agtattattg gtgagtaaat gcataggctg aacagttcta ctcacagctg cacgcaaact
     2461 catgccatta atcggatggt aaattaattc cgcgcgtggc tgcggataga atatactttt
     2521 cttattgaca tgatataaga ataactgcat gccagcattt aattctagtt tgtttttcca
     2581 tgtaaaacga taatcggcaa acgcataaaa atctagcgta tttacttttg gattgacata
     2641 tttttctgat tgaatgatgt tgtttgtgct atcataaacc ttagcgtcga tcgtattgat
     2701 gtgaaatgtt cttgcattaa atttgatgcc gtaattaaga tgatgcatgt cttttatagc
     2761 atgttcaagc atcgtcagcc atttgtgctc attaatttta gaggcattat tgtattgtgt
     2821 tttgtgaatg tcattagata aatttgtata gttacgctcg acgattgcaa attgttgttt
     2881 agctgataaa ttatagaaac tataagcgta agcattgtaa attgtttgtt tttttaagtt
     2941 tgttttccat tctatgctat tcgcaatatt ctgccagctc aattttcttt gtgtataatc
     3001 tttactatat tttgtagtgc gtttatattc ttttccttct ccaaatgtaa aaaaatcact
     3061 gcctgtatat aggctccatg ataatgtatt tttatcattg acatgatgtt gtatggctgc
     3121 gttgatatcg taaaaaaaat attttaaagt gccgttttcg ccatttcttt tatattgttt
     3181 tgcagcaata ggttctgtaa aaaatcctgc atgacaatcg cgtatcgaaa aattaaatga
     3241 tgttttttct ttctttaatg gtccttcgat atgaaatttt tgtgtgataa aactaatact
     3301 gtaatcacct ttccaagtct ctgtattgcc aaagttgggt tcgatattta atacagaaga
     3361 tagccttccg ccatactgta caggaatata gcttttataa atatccatct tcttcaatga
     3421 ttctgtagtg aaaacggata aaaaaccata aatatgcgat gcattaaata cagtggcatg
     3481 atgaaaaagc attaagtttt gatcagggct tccgcctcgt acaaatatgc cacgtgctcc
     3541 ttcttgtcca ctctgaattc caggtgttag cagtatagct ttcatgatat ctttttcgcc
     3601 gaaaaacgca ggcaactttt caacactcat gctactcagc gttgtcacac ctgaggtgct
     3661 catcgtggct gttttgttta ttttttctgc tgccacagta acgccatcta actgaactat
     3721 ttcttgtagt gatatatcaa ttgtactatc ttttgtgatg ttgacttctc tgaaaattgt
     3781 gttgaagccg atgcaagaga attcaaaatt atattttcca gctgctaatc ggatttcaaa
     3841 tttgcctaat gaatctgtag tggaaaatgt tcttgatgtt gaatcataaa tattagcaaa
     3901 tgcaattgct ttgtgtgttt gttgtgataa tatattgcct ttaaatgtgt atgtatttgt
     3961 tgcataacat actaagccga aaagacttaa tagtacatac atacagctgc tataaaggta
     4021 gtttttcata gtatagtcat tactaattta caaaacgaat aatgtttatt gcattttttt
     4081 tagcgcaaat catcatttta ggcatatatt acgtatgcat gatgctagtg ttaagtacct
     4141 tatattactt gtgattagca tcataattta ggtaatttgc atgatataaa tcatcttagt
     4201 acataactaa aaacatattc tatatgctaa ttaaagtgaa aatttgtatt gtaaaaatag
     4261 ctatttagaa ttagctgatt tacactgaaa aaatacaaaa atgaaattta aattaatcaa
     4321 accaattttt aactttttaa acaattttag tatgagaaaa tggatcatcg cgcctgtatt
     4381 agcatcatta gtgatgttag gcgtaagcgc ttgtaaacaa aacgagagcg cacctgcaac
     4441 gatgtcatct gcggaagccg aaaaaatcgt agcggaagga aattatgaag aagcagaagt
     4501 gactgcacca ccattagtgc ctgcaccaat tggtgaccgt aaagcaaaaa aagtaatcat
     4561 cagactagaa actatcgaga aagtaggcga gttggaagat ggtactcaat acactttctg
     4621 gactttcaat ggtactgtac caggttcatt cattcgtgtt cgcgtaggtg acgaagtaga
     4681 gcttcactta aaaaacaacg aaaacagtac tgtctctcac aatatcgacc tacatgctgt
     4741 aaatggtcct ggtggtggtg ctgaaggcac tttggttgca cctggcaaag aagcagtagt
     4801 caactttaaa gtaactaatc ctggcttata tgtttaccac tgtgctacag caccagtacc
     4861 agaacatatt gctaatggta tgtatggttt aatcttggta gagcctgctg gtggtttgcc
     4921 taaagtagat aaagaattct acattatgca aggcgacttc tatacatctg gcaaaaaagg
     4981 agataaaggt ttgttgagct ttaacttaga taaaagtatt gctgaaaatc ctgactatgt
     5041 agtattcaat ggtaatgcaa cttctttgtt aggtaaaaac gtattgcaag caaaaactgg
     5101 tgaaactgtt cgtatctact ttggcgttgg tggtcctaac ttaacttctt catttcacgt
     5161 aattggtgaa atatttgaca aagtgtatat cgaagcgggt actaaagtaa acgagcacgt
     5221 acaaactaca atggtgcctg ctggtggtgc agtaatagtt gagttcaaag ctacagttcc
     5281 tggcgattat gtaatcgttg accactcttt atcaagagca tttaacaaag gtgctatcgg
     5341 taaaataaaa gttactggcg atgaaaatcc tacagtttat aaatcaaaaa aataaacaaa
     5401 gattttaatt agtcaataaa aaaataagat gaaatctctt ccatatcttc taatacttct
     5461 actgtttatt gcttgtgaaa caaaacaagt aaataaacag gtattagaag attggaaaga
     5521 tgaaatcaaa ccagtacagt catctaaatt aatgcagaaa attgactcag gaacttatca
     5581 gctatttttt ggaaaagatt ctggcggact tgcaattgta cctacttttt atttagatga
     5641 aacagcagtt acaaatgctg aatatttaac ttttctaaaa tcaaatccac agtggacaaa
     5701 aagtagagtg ttgcatattt tcgcagattc tacatattta agcaaatgga aaagtgatta
     5761 tgaaattcca gctaacgctg acccaaacgc acctgttaca aacgtttctt ggtttgccgc
     5821 aaaagcttat gctaaaagcg taggtaaaag attgccaaca gtggatgaat gggaatttgc
     5881 agcattggcg agccgtgatg caaaaaatgg gaaaggcact cctgaatatg ataactatct
     5941 gatgaaatca tatttgaata gaggaatgta cttgaagcca gtaaaacaaa gcccagccaa
     6001 tttctatggc atctacgata tgtttggtgt tatttgggaa tggacagaag attttaattc
     6061 tgtaatgatt accaatgact ccagaaacaa tgcctcagat gaaaatttat tctgtgcagg
     6121 ctcatctaat tccacttcag atttacgcaa ttatgcagca ttcatacgtt atgccatgcg
     6181 tggaagtgtg aaagcaaatt attgcatcaa taattatggc ttcagatgtg caaaagatgt
     6241 agaagcttct aaaaaataat aacatgaaac gaatactaat tttaagcctt tctattttat
     6301 tgttctttgc ttgtaaaaat aatacaagca acaacgatat ttcagccgat tcggtgtacc
     6361 atttgccttc acaatgggaa aaccaagcag gtcaaaaaat gaaaatggct gacttaaaag
     6421 gaaaagtagt agtgatggtg atgatttatt ctacctgcaa aacggcatgt ccaattttaa
     6481 cttccgaaat gggagagatt gccagcagat tagctgatgt aagcccaaaa gatattcgtt
     6541 ttgtaatggt gtctattgac ccacaaacag atacgccaga acgtatgaca gcctatttaa
     6601 agaaaaataa ttttagcggc gaacaatggt tgttcttaag aagtagcgaa gaagaaacac
     6661 gcgaattagc aaatgtactt tccgttaagt ataaagaaat ttctccagtc gatttttctc
     6721 attctaatat tataagcgta ttctctaaaa acggcacact tgcattccaa aaagaaggac
     6781 ttggaattga cgttgaaggc actgtctcag aagtaaggaa gcagcttcaa taaaagcttc
     6841 cgcttagtat tttttaatta tttaaaatat taatatgaaa tgcaaatttt tcatagtagg
     6901 attcctattg cttgcaatga accaactcta cgcaaaagta gatagtataa aattgaattt
     6961 cgactttaga acaaaagtag aaatcgatca cggcaatcac actctttttc cagaaggcat
     7021 aaagactaaa acgcacgtgt caagcagagc acgaataggg tttgatggct atttcttttc
     7081 aaaactacaa ttagctattg cactacaaga cgcaagaact tggggcgagt cttctacaac
     7141 aggcgtaaaa cttttaaaca acatcgttta tgaagcgtgg agtgcctatt ggttcacgcc
     7201 aaaaattgca ataaaaattg gtcgtcaaaa attagcctat gacgacgaac gccttattgg
     7261 tggtttagat tgggcaatgc aaggcagaac attcgatgca ttaaaaggta tattcaaatt
     7321 agattctaat gcaaattctc aaatcgaatt agtcgcaacc tacaacaacg acgaaaatac
     7381 aaaaaatgat actttgggtc gaacagttta tggtatctct gaagctggtg aaaccacaaa
     7441 atcattgcaa atactacact atagttataa aaatgcaaag aaatttcaag tgtcagcaat
     7501 agctgtaaat aatgttctgc aaaacaaatc aggtaagtac tatgacatga ttaccattgg
     7561 tatgaatgta aaaaaatact tcaaacaagt cggtatttat ggctcagctt attatcaagg
     7621 tggaaaaaat acactcggtc aaaaaa
//
LOCUS       DAOQQV010000122         7425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4015_length_7425_cov_7.790231, whole genome shotgun sequence.
ACCESSION   DAOQQV010000122 DAOQQV010000000
VERSION     DAOQQV010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4015_length_7425_cov_7.790231"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLL59_10690"
     CDS             complement(<1..1125)
                     /locus_tag="PLL59_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMY24316.1"
                     /translation="MNNSTEFLSAYNKKDKSIGVIGLGYVGLPIALELAKKFKVIGFD
                     INQKRVEMMQNNIDPSNELSSSAFEGTDIVFTAETDVLKMATFFIVAVPTPVDEHNVP
                     DLKPLIGASNTIAKVIKKGDYVVFESTVYPGCTEEDCVPIIEEKSGLKFIEDFKVGYS
                     PERINPGDTLHTLTKITKIVAGCDDESLENIAAVYGAIIEPGVYKASSIKVAEAAKVI
                     ENTQRDLNIALMNELSQIFSLMNINTYDVLEAAGTKWNFLKFQPGLVGGHCIGVDPYY
                     LTYKSNELGYKPQIILSGRSINDGMPNYIAKKIVQFLLKKGKNPSQCRALIMGATFKE
                     NVTDIRNSKVFNLYNELKDFQLDVEMVDALADKHEVEEEYG"
     gene            1240..2430
                     /locus_tag="PLL59_10695"
     CDS             1240..2430
                     /locus_tag="PLL59_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HMY24317.1"
                     /translation="MSIIYSIAIYLYSALVWISSFFNEKAKSWVNAQKNWKKILLNSE
                     KTNEHIIWIHCASAGEFEQAIPIINAIKKKKANYKIYVSFFSPSGFAMYKDSKQADYF
                     FYFPLDTAANAKYVVDFLQAKYVIFIRNEIWLNVLSLLYQKKTPTYLVNANLKQRRNL
                     LYRFYLDKTFPLFTKIFSTQDDGNTKLEQVVQNKNQIFSDKILTDFCRDSIVVILGSS
                     WQEEEKMMARFFIKNNAKIKIIIAPHEYGDGKLEQLNSLFTSEEQSKSTIISSYSNYQ
                     FGNTSAILFLDRKGILKYAYRYASIAVIGGGFNSGTHNISEAAVYGIPILFGPNYTKF
                     EDANDFIQLETAHSFKQYEAFEQQLLIYLNDEILRNCIKEKLDVYFDKHNNVAEAIVD
                     DIIK"
     gene            complement(2477..3898)
                     /locus_tag="PLL59_10700"
     CDS             complement(2477..3898)
                     /locus_tag="PLL59_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24318.1"
                     /translation="MEIRNALQKIENYLTVVSNLNRTILSEGTMSRDELLLMKKYLYT
                     SIDRIEDIERSLIIDKSVDKSFVPTALVDNTVKPTISKEKEIVAVETNEHLDEVEAVL
                     TATQTEDLHEFVEEIQTENDNKLVEIVGEISTTTIENKSDELPILTSGDAETFVESII
                     TEDYALNSAETTVVETVLSVAEEAKPMAEKTSFVDSIITEDYSLNKLFESKEENNANL
                     LVNEIEEPIIPFKEVKSEVQNIITEDYALNSNEQNVEIVAEVQKQEVQTNEDELDPID
                     TTLVAQFENKTPLTFAEQLENSKNEVSLSDQLEQKLNATQQAQLFEKFDDGKDDFHET
                     FLQTKQSEITQIKTENPISTAYDSGNVLVAEKIETVVDELTPSALNDVFKSQTIHDVL
                     QAKSAKTISESIALNDKFIFVRELFGNHFGEYEMGLKQLETQTTYEQAENFCKEKLWN
                     KYNWNDKTNAVERFMAVLQKRFF"
     gene            3951..4532
                     /locus_tag="PLL59_10705"
     CDS             3951..4532
                     /locus_tag="PLL59_10705"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HMY24319.1"
                     /translation="MFLEPNVNKSKSGWIEVVCGSMFSGKTEELIRRLKRAKIANLKV
                     EIFKPLIDTRYHETEVVSHDANSITSTPVSSSQNILLLSNDVEVVGIDEAQFFDDGLP
                     EVCEQLANKGIRVIIAGLDMDFLGRPFGPIPALLAKAEYITKVHAICVRCGALANHSY
                     RLVENESKVLLGEKESYEPRCRACFSNSMQQEP"
     gene            4543..5052
                     /locus_tag="PLL59_10710"
     CDS             4543..5052
                     /locus_tag="PLL59_10710"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HMY24320.1"
                     /translation="MKVAGFEIENKINPNNVDLFLNGVAIRSKFFIQTYIIALYAAKP
                     IQQENIAIESNIERSLRMLIITPLATSKAVSENIQNGMKEGLGNLYMQQKDLVDDLRK
                     VIETSNVQFKDSIDIYYTSDKELKLYKNNQEIYSNKNGQLFAETIFGMYVGKKPKDKK
                     IKEALLKGF"
     gene            5104..6549
                     /locus_tag="PLL59_10715"
     CDS             5104..6549
                     /locus_tag="PLL59_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMY24321.1"
                     /translation="MKKLILLVLASLLIFIGIISFKTITAPHLQIKIKHNPAPVLSDN
                     ALQHFQEAISFPTISYNDTLQWKAEPFIQFRQFLEKTYPLVHQKLNREIVSNYTLLYK
                     WQGKDTTLAPFILMAHQDVVPIETATQNQWTCQPFAGTIKDGYIWGRGTTDDKINLIS
                     ILESTEKLLSENFQPTRTVYFVFGHDEEIGGKQGAVKVAALFKQRNIKADLVMDEGGI
                     VTKEKVPGINKAVALIGTAEKGYMSLLFSVNKKGGHSSQPDKETAIDILTKALVRLRE
                     NPFEANFVEATQDFMRYLGPEMNFPNNMAFANAGLFKSLIDKNYSKTGTGNAMIRTTA
                     VPTIINAGIKDNVIPTLATAVVNFRLLPGDSSAHIISKVNEIINDKRVIVSKFDDNIS
                     EASRTTSVKSIAFTRVDSLIKKSYAHVVSAPFLLIGATDSRHFADVSENILKFSPMLD
                     PIGFHGIDERVSLESFRHSLWFFEQFLRSCH"
     gene            complement(6563..>7425)
                     /locus_tag="PLL59_10720"
     CDS             complement(6563..>7425)
                     /locus_tag="PLL59_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY24322.1"
                     /translation="EFGLDLEDNSALNFIDMLSVSESNEVELYGDSDERYSIKGGVVS
                     LINAMATAVGNIELEHKLIEINKAGDGYSLIFENGKSAYADFVIITIPFTVLRDVKFN
                     VGAIAPQKLKAIKELGYGQNAKLFLGFDERLWRTKYKTIGYLFNDKIHNGWDSSYGQT
                     DNKGAGIYTVFLGGKDAIYMAENKKNTQQFVDMYLTILDSIYPGIKNKYNDIADIAWW
                     PKSKIVKGSYSAFYPGQWTGVMPYINEPIGNILFAGEHCSDEFQGFMNGGAQTGKDIA
                     LQLLKKIKAT"
BASE COUNT         2409 a         1287 c         1192 g         2537 t
ORIGIN      
        1 accatattct tcttccactt cgtgtttatc tgctaatgcg tcaaccattt ctacatctag
       61 ttggaaatcc ttaagctcat tgtataaatt aaacactttt gagtttctga tatctgtcac
      121 attttcttta aaagttgcgc ccataattag cgctctacat tgcgatggat ttttgccttt
      181 ttttaataag aactgtacta ttttttttgc aatataattc ggcattccat cattgatgct
      241 tctaccactt aatataattt gtggtttata gcctagttca tttgatttat aggttagata
      301 gtatggatca acgccgatgc agtgaccacc tactaaacca ggctgaaatt ttaagaaatt
      361 ccattttgtt ccagctgctt ccaatacatc gtatgtatta atattcatca agctgaatat
      421 ctgagacagc tcattcatga gcgcaatatt taaatcgcgt tgtgtattct caattacttt
      481 agccgcctct gctactttaa tagaagatgc tttgtaaaca ccaggctcga ttatagcacc
      541 ataaacagca gcaatatttt ctaaggattc atcatcacag ccagcaacga tttttgtgat
      601 ttttgtaagt gtatgaagtg tatcaccagg attgatacgt tcaggcgaat agccaacttt
      661 aaaatcttct ataaatttca atcctgattt ttcttcaata attggtacgc aatcttcctc
      721 tgtgcaacct ggataaacgg tagattcgaa aacgacgtaa tcgccttttt taatcacctt
      781 tgcaattgta ttggaagctc caatcaatgg ttttaagtca ggcacattgt gttcgtcaac
      841 tggtgttggc acggcaacga taaaaaatgt tgccattttt agcacatctg tttcagcagt
      901 aaatacgata tctgttcctt cgaaagcgga acttgataat tcattactcg ggtcgatgtt
      961 gttttgcatc atttccacgc gtttctggtt gatgtcaaaa ccaataactt taaatttttt
     1021 cgctaattct aatgcgatag gtaatccaac atatccaagt ccaattacgc ctatggactt
     1081 atcttttttg ttatatgcgg acagaaattc tgttgagttg ttcattttat gtctacgaga
     1141 tgggcaaatg tacaaaaaaa atcgtaaaat attttagcat aaaccgttaa caaataataa
     1201 gtttgcccta tattttttga tatctaagaa caaatattca tgtctattat ctattctatt
     1261 gctatttatc tctattccgc attagtttgg ataagcagtt tttttaatga aaaagccaaa
     1321 tcatgggtaa atgctcaaaa aaattggaaa aaaattctat tgaattcaga aaagacaaac
     1381 gagcacatta tttggataca ttgtgcttcc gctggtgaat ttgaacaagc aatacctatc
     1441 atcaatgcta tcaaaaaaaa gaaagcgaat tataaaatct acgtttcttt cttttcaccc
     1501 tctgggtttg caatgtataa agattcaaag caagctgatt acttttttta ttttccctta
     1561 gacacagcag cgaatgcaaa atatgtcgtt gattttttac aagcaaaata tgtcattttt
     1621 atcagaaacg aaatatggtt gaatgtgtta tcattactct atcaaaagaa aactccgact
     1681 tatttagtca atgcgaattt gaaacaaaga agaaatctat tatatcgttt ttatcttgat
     1741 aaaacatttc cattatttac taaaattttt agcacacaag acgatggcaa cacgaagtta
     1801 gagcaggtcg ttcaaaataa aaaccaaata ttttcagata aaatcttaac agatttttgc
     1861 cgtgatagta ttgttgtcat attaggaagc agctggcaag aagaagaaaa gatgatggct
     1921 cgttttttta ttaaaaacaa tgcaaaaata aaaataataa ttgctcctca tgaatatgga
     1981 gacggcaaac ttgaacaatt aaactcactg tttacatctg aagaacaatc taaatcgaca
     2041 atcatttctt cctattcaaa ctatcaattt ggaaacacat ctgctatttt atttttggac
     2101 agaaaaggaa tattaaaata tgcttataga tacgcttcaa tagcagtaat tggtggtggt
     2161 ttcaacagtg gcacgcacaa tatttcagaa gctgctgtgt atggtattcc aattttattt
     2221 ggtccaaact atacaaaatt tgaagatgct aatgatttta ttcaattaga aactgctcat
     2281 agttttaaac aatatgaagc atttgagcag caattattaa tttatctaaa tgatgaaata
     2341 ttacgtaatt gtatcaagga aaagcttgat gtatattttg acaaacataa taatgtagca
     2401 gaagcaatag ttgacgatat tataaaataa aaatcccgtg catgagcacg ggaaattgat
     2461 tcatacattc ctcgacttag aaaaatcgtt tttgcagcac tgccataaag cgttccactg
     2521 cgttcgtttt gtcgttccaa ttgtatttat tccataattt ttctttacag aaattttctg
     2581 cttgttcgta agtcgtctgt gtttccaatt gtttcaagcc catttcgtat tcaccaaaat
     2641 ggttgccaaa taattcacga acaaaaataa atttatcatt taatgctatt gattcgctaa
     2701 ttgtttttgc tgatttagcc tgtaaaacat catggattgt ttgtgattta aatacatcat
     2761 ttaatgctga aggtgttagc tcatctacaa cagtttcaat tttttctgcc accaaaacat
     2821 ttccactatc atacgcggta gaaattggat tttctgtttt gatttgcgta atttctgatt
     2881 gtttagtttg caagaatgtt tcatgaaaat catcttttcc atcatcaaat ttttcaaaca
     2941 attgtgcttg ttgagtagca tttaatttct gttcgagttg atctgatagc gaaacttcat
     3001 ttttgctatt ttctaattgc tctgcaaaag ttaatggtgt tttattttca aattgagcta
     3061 ctaatgtcgt atctatagga tctagctcgt cttcatttgt ttgaacttct tgcttttgaa
     3121 cttcagctac tatttccacg ttttgctcat tgctattaag tgcataatct tctgtaatta
     3181 tattttgcac ttcgctcttc acttctttaa atggaataat aggttcttct atctcattga
     3241 ccaacagatt agcattgttt tcttctttgc tttcgaagag tttgtttaat gaataatctt
     3301 cagtaatgat actatcgaca aatgaagttt tttctgccat tggtttggct tcttcagcaa
     3361 cactcaatac tgtttccact actgttgttt cagcactatt taatgcataa tcttctgtga
     3421 taatactttc tacgaatgtt tctgcatcgc ctgaagttag aattggcaat tcatcgcttt
     3481 tattttctat tgttgtagtt gaaatttcgc caactatctc caccaattta ttgtcatttt
     3541 ctgtttgaat ttcttcgaca aattcatgca aatcttctgt ttgtgttgct gtcaaaactg
     3601 cctccacctc gtctaagtgt tcattcgttt ctacggcaac gatttctttt tctttgctta
     3661 tcgttggctt tacggtatta tcaacaagtg cggtaggcac aaaagattta tctacgctct
     3721 tatcgattat tagcgaacgt tcgatatctt caattctatc gatgctggta tataaatact
     3781 ttttcatcaa aagtaattca tctctagaca tagttccttc tgacagaatt gttcgattta
     3841 gattggaaac tacagtaagg tagttctcaa ttttctgcaa agcgtttctt atttccatct
     3901 gtttttgtat tttgatgtcg aatttataag gtaaaataca aatttcatac atgtttttag
     3961 aaccaaatgt taataagtct aaatcgggtt ggatagaagt ggtgtgcgga tcgatgtttt
     4021 ccggcaagac ggaagaactc attagaaggc taaaacgtgc caagattgcc aatttaaaag
     4081 tggaaatttt caaaccatta atcgacacca gatatcatga aacagaggtt gtttcgcatg
     4141 atgccaattc tataacgagc acacctgttt cgagctcaca aaatatatta ctattatcta
     4201 atgatgttga agtagtcggc atcgatgaag cccaattttt tgatgatggc ttgcctgaag
     4261 tgtgtgagca attagcaaat aaaggtattc gagtaattat tgctggttta gacatggact
     4321 ttttaggcag accattcggt ccaataccag cacttctggc aaaagctgaa tatattacca
     4381 aagtacatgc catttgtgta agatgtggag cattagccaa tcattcctat cgcttggtag
     4441 aaaatgaaag caaagtttta ttaggagaaa aagaaagcta tgaaccgcgt tgtcgagctt
     4501 gtttttcaaa tagtatgcaa caagaaccgt aaatttatag gtatgaaagt agcaggtttt
     4561 gaaattgaaa acaaaataaa tccaaataat gtagatttat ttttgaatgg tgtggcaatt
     4621 cgttctaaat tttttattca aacttatatc attgctcttt atgcagcaaa accaatacag
     4681 caagaaaata ttgccataga aagcaatatc gaaagaagct tacgtatgct tattattaca
     4741 ccattagcaa catcaaaagc ggtaagcgaa aacatacaaa atggcatgaa agaaggcctt
     4801 ggcaatttat atatgcaaca aaaagatttg gtagatgact tacgcaaggt aatagaaaca
     4861 tcaaatgtac aattcaaaga cagtatcgat atttattata cgagcgataa agaattaaaa
     4921 ttgtataaaa ataatcagga aatttattcc aataaaaatg ggcaattatt tgctgagaca
     4981 atttttggca tgtacgtagg taaaaaacca aaagataaaa aaatcaaaga agcattattg
     5041 aaaggatttt aataacagtc caagtttacg ctttagaata atacaaaaat catcaacaac
     5101 aacatgaaaa agcttatatt gttagtactt gcttcattac ttatttttat aggaattatt
     5161 tcttttaaaa cgattacggc accacactta caaataaaaa taaaacacaa tccagcgccg
     5221 gtgctatcag ataatgcctt acaacatttt caagaagcta taagctttcc aactatatca
     5281 tataatgata cgctacagtg gaaagccgag ccttttattc agttcagaca atttttagaa
     5341 aaaacatatc cattagtgca ccaaaagtta aatcgtgaaa tcgtcagcaa ttatacgctt
     5401 ttgtacaaat ggcaaggcaa agacacaaca ttggcgccat ttattttaat ggcacatcaa
     5461 gatgtggttc ctattgaaac tgctactcaa aatcaatgga cttgtcaacc gtttgctggc
     5521 actataaaag atggatatat ttggggacga ggtactactg acgataaaat aaatttgatt
     5581 tccattttag aaagcacaga aaaattacta tcagaaaatt tccaacctac gcgaactgtt
     5641 tattttgttt ttggacatga tgaagaaatt ggaggcaaac aaggtgcagt taaagttgct
     5701 gctttattta aacagagaaa cattaaagca gatttggtga tggatgaagg tggcattgta
     5761 acaaaagaaa aagtgcccgg cataaacaaa gcagttgcat taattggtac agctgaaaaa
     5821 ggctacatgt ctttattatt ttctgtcaat aaaaaaggtg gacattcttc gcaacctgac
     5881 aaagaaactg caatagatat tttgacgaaa gcacttgtcc gtcttcgtga aaatcctttt
     5941 gaggcaaatt ttgtagaagc tacgcaagac ttcatgcgtt atttaggtcc tgagatgaat
     6001 tttccaaata atatggcgtt tgcaaatgct ggattattta agtctttaat tgataaaaat
     6061 tattcaaaaa caggaacagg caatgcgatg atacgcacta cagcggtacc aacaatcatc
     6121 aatgccggca ttaaagataa tgtcattcct acattagcca cagcagtagt caactttcga
     6181 ttattgcctg gcgactcttc agcacacatc attagcaagg taaatgaaat cattaatgac
     6241 aagcgtgtaa tcgtatctaa atttgatgat aatatttctg aagcctcccg cacgacttcg
     6301 gtgaaaagca tagcatttac acgtgttgac agcttaatta aaaaaagtta tgcgcatgtt
     6361 gtaagcgcac catttctatt gattggtgcc acagattcac gacattttgc tgatgtttca
     6421 gaaaatattc taaaattcag tcctatgctt gatccaattg gatttcatgg cattgatgag
     6481 cgtgtaagtt tagaaagttt tcgtcattca ctatggtttt ttgagcaatt tttaagaagt
     6541 tgccattagc atatatacaa aattatgttg cttttatttt tttcaacaat tgtaaagcaa
     6601 tatcttttcc cgtttgtgcg ccaccgttca taaaaccttg aaactcgtcg ctacaatgtt
     6661 cgcctgcaaa taaaatatta ccgataggct cattaatata tggcattaca cctgtccatt
     6721 gacctggata aaatgcagag taagagcctt tcactatctt ggattttggc caccatgcaa
     6781 tatccgcaat atcattatat ttatttttta ttcctggata aatactatct aatatagtca
     6841 aatacatatc gacaaattgt tgtgtgtttt ttttattctc tgccatatat attgcatctt
     6901 ttccgcctaa aaaaacagta taaatacctg ctcctttatt atctgtttga ccataagaac
     6961 tgtcccagcc attatgtatt ttatcgttaa aaagatagcc tattgtttta tattttgtgc
     7021 gccataatcg ttcatcaaat ccaagaaaca gcttagcatt ttgaccatat cctaattctt
     7081 taattgcttt taacttttga ggtgcaatag cgccaacatt aaacttcaca tcgcgcaata
     7141 ctgtaaatgg aatggtaatg ataacaaaat cagcataagc cgattttcca ttttcaaaaa
     7201 tcaaggaata gccatcgcct gctttattta tttctattaa tttatgttct aactcgatgt
     7261 ttccaacagc agtagccatt gcattaatca gtgacacaac gccgccttta atggaataac
     7321 gttcatcact atcaccatat aattctactt cgtttgattc gcttacgctc aacatatcga
     7381 taaaattcaa tgctgaatta tcttctaaat ccaaaccaaa ttcgg
//
LOCUS       DAOQQV010000123         7317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4107_length_7317_cov_6.685348, whole genome shotgun sequence.
ACCESSION   DAOQQV010000123 DAOQQV010000000
VERSION     DAOQQV010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4107_length_7317_cov_6.685348"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLL59_10725"
     CDS             complement(<1..304)
                     /locus_tag="PLL59_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HMY24323.1"
                     /translation="MGYFTETSGQTGNLKTIYLPGLNGLRAIAALAVVISHITLGLGG
                     FGLNNKIFGTDLEGNPSGSALAGYGVTIFFTLSGFLITFLLLKEKEKTTKLNVKNFY"
     gene            complement(411..1445)
                     /locus_tag="PLL59_10730"
     CDS             complement(411..1445)
                     /locus_tag="PLL59_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMY24324.1"
                     /translation="MEWNAAIVKYKKQERIAVYFKYDVNLIQQIKKYDDAQWSYTLKA
                     WHLPNNEENRKIFMLENAVLHADKQAKIDQFSRWLHSKRYSENTIKTYIDALKSFLIY
                     FNTKQIETITNDDLIFYNNDYILKNEFSSSYQNQIVSAVKLFFRTIENKKMNEELIHR
                     PKRERKLPHILSKEEVKQILNAHSNIKHKAMLSLIYSCGLRSGELLSLKPLDIDSKRN
                     LILITQAKGRKDRIAPLSNKTIELLRQYFIAYKPTTFLFEGQYKGTQYDARSLQQVLK
                     QALDKAHIQKPVTLHWLRHSYATHLLEAGTDLRYIQELLGHSSSRTTEIYTHVSTHNI
                     RNIVSPFDTL"
     gene            complement(1593..2858)
                     /gene="bioA"
                     /locus_tag="PLL59_10735"
     CDS             complement(1593..2858)
                     /gene="bioA"
                     /locus_tag="PLL59_10735"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HMY24325.1"
                     /translation="MNLSEKDTNYIWHPFTQHQTAAPNIVITHGNAASLYDENGKEYI
                     DAVSSWWTNLFGHANLEISNAIKNQLDQLEHVIFAGCTHQPAIDVAETLLKILPNNQN
                     KIFFSDNGSTSVEVALKMAIQYWHNQQIEKNTIIAFKNAYHGDTFGAMAVGARGIFNQ
                     AFEPYLFDVEFIDLPTASNINHLSNQFLQIIQSKKVAAFIFEPLVQGSAGMLMYDAIY
                     LDKLIAIAKQNNIICIADEVMTGFGRTGKNFASDYLQHKADIFCMSKGITGGFLPLGI
                     TSCTQKIYDAFLSSNKLKTFLHGHSYTANPLACAAANASLNLLSNAQDMINELVVWQS
                     EMKANLLQHHKVENIRQTGTIIAFEVKSNFSNSYFNNIRDVLYDNFINRGILLRPLGN
                     TVYVMPPYVITKTQLRQIYDVIIEVLDIV"
     gene            complement(2845..3525)
                     /gene="bioD"
                     /locus_tag="PLL59_10740"
     CDS             complement(2845..3525)
                     /gene="bioD"
                     /locus_tag="PLL59_10740"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HMY24326.1"
                     /translation="MKGFFITGIGTDVGKTVVSAIVVEALQADYWKPIQCGNLDFTDS
                     DFIEEYTLHHQTIHDEAYLFEDPVSPHLAAYLSNKNIDNQFIKLPKSNNYLIVEGAGG
                     LMVPLSQNYLIIDLIKQLQLSVIVVSMNYLGSINHTLLTIESLKYHNIDIAGIVFNGD
                     ENSSSETFILQHTGVRLLGKIAKADILSKGFIAHQASSIRKNLLPLLSPLPNQDTDTN
                     LPLDYESF"
     gene            complement(3529..4668)
                     /locus_tag="PLL59_10745"
     CDS             complement(3529..4668)
                     /locus_tag="PLL59_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HMY24327.1"
                     /translation="MNAVERINHFLLQRNNEHLFRKLIITNDLIDLSSNDYLGLSRSA
                     FIRQQVQDDFKNYQFHKSGATGSRLLNGNSLLCEEIEERLAQIHHAEAALLFNSGFDA
                     NVGLISTVIRPKDTIFYDEMVHVSIHQGMRLSGAKLIPYKHNQLGDLIQKLNNSDYDI
                     AFLITESVFSMDGDKANLSELAEICAQFRLNLIVDEAHAGGLFGINGSGLCNVSAVEE
                     KCFARIYTFGKAIASHGAAILGTQLLKDYLINFSKNFIYTTALDTHNLLSVKHSYSFI
                     QSNRNQLFKLNNLNNYFYDNINKLASRFEVCGEGPIFGIIVPGNKNCKEIARYLQMNK
                     LDVRAILSPTVPLGRERLRVVLHSFNTKDEIDRLFSLLLDYKTVI"
     gene            complement(4690..5688)
                     /gene="bioB"
                     /locus_tag="PLL59_10750"
     CDS             complement(4690..5688)
                     /gene="bioB"
                     /locus_tag="PLL59_10750"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HMY24328.1"
                     /translation="MQVRNNWTFEEIKAIYQKPLLELMYEASAVHQKFNRIGEVQVNT
                     LLSVKTGGCPEDCAYCPQAARYNTGLKAHKLLDVDDVISKAMEAKENGSTRFCMGAAW
                     REVRDNKDFDKVLEMVSGVNQLGMEVCCTMGMLTEEQAIRLKNAGLYAYNHNIDTSEE
                     HYDKVITTRTYQDRLNTIGNVRKAGISVCSGGIIGLGENEDARIGMIYTLATLPQHPD
                     SVPVNALVAVEGTPLEDMKRVSVFDMVRMIATARITMPLTLVRLSAGRLDMPVAEQAL
                     CFFAGANSIFAGDKLLTTPNPSFADDQSMFELLGLRPRVAFKQEEVSAETSCCANH"
     gene            complement(5721..6212)
                     /locus_tag="PLL59_10755"
     CDS             complement(5721..6212)
                     /locus_tag="PLL59_10755"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HMY24329.1"
                     /translation="MLCIIATFIFISCVRKHRFDTDKKILEEKKMATVLTDVFLMEAY
                     VNEQLKWRNSDSVSLVRKIYYPDILKHHKVDSTSFFATLRYYQAHPKEYSSLLVAVDS
                     LLYKIKALDTTTAPIDNNVVIPPNINDISSYKKQEQAMGEEYIKNNPVFKNFKRKNGM
                     DSK"
     gene            6253..6948
                     /locus_tag="PLL59_10760"
     CDS             6253..6948
                     /locus_tag="PLL59_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMY24330.1"
                     /translation="MPTISVLQDITKYAKAKNAVLVAISKTKTVDDIQQIYNAGQRVF
                     GENKVQELVQKYELLPKDIQWHMVGNLQSNKVKYIAPFIQLIHSVDSLKLAIEINKQA
                     LKHNRVIDILLEIHIAAETNKSGFSEDELIDHLDINAFNSLQNIRIVGLMGMATNTKS
                     QVLIKNEFSGLKQYFDKIKQQYFSKSDEFSILSMGMSGDYQLALEEGSTMIRIGSAIF
                     GEREIKKNPNIPI"
     gene            complement(6941..>7317)
                     /locus_tag="PLL59_10765"
     CDS             complement(6941..>7317)
                     /locus_tag="PLL59_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HMY24331.1"
                     /translation="VDRLLEIGVNKFSISDTIGVATPELIQSVFSILSEDFPEIGFGA
                     HFHTTPTTWREKVEAAWNAGCTRFDGAIKGYGGCPMATDKLTGNMPTENLVAYFEENN
                     IEHGLNKEAFINAMQIANTIFK"
BASE COUNT         2311 a         1286 c         1210 g         2510 t
ORIGIN      
        1 tgtaaaaatt tttaacatta agttttgttg ttttttcttt ttctttcaac aacaggaaag
       61 ttattaaaaa accactcaaa gtaaaaaaga tagttactcc gtaacctgca agagccgaac
      121 cgcttggatt accttcaagg tctgttccga atattttatt gttaagtcca aaacctccaa
      181 gtcctaatgt gatatgtgaa ataacaactg ctaatgcggc aattgccctt agtccgttaa
      241 gtccaggcaa ataaattgtc tttaaatttc ctgtctgacc gctggtttct gtaaaatagc
      301 ccataacttg tttatatacg ctacaaagct tcgttaatgc aaacacttat tgttggatat
      361 gcgatacttg gtagcgtatt attaatttca acgaatataa gaaaattaaa ttataaagta
      421 tcaaatggag atacaatatt tctaatatta tgtgtgctca cgtgtgtata aatttctgta
      481 gtacggctgc tgctatggcc aagcaattct tgtatatatc gcaggtcggt tccagcttcc
      541 aatagatgcg tagcataact gtgtcggagc caatgcaatg ttactggttt ttgaatgtgt
      601 gccttatcta atgcttgttt taatacttgt tgtaaacttc ttgcatcata ttgagttcct
      661 ttatattggc cttcaaataa aaaagtcgtt ggcttatagg ctataaaata ttgtcttaat
      721 aattcaatag ttttgttgct taaaggcgca atcctgtctt tgcgcccttt tgcttgcgtg
      781 atgagaatta aattgcgctt actgtcgata tctagtggtt ttaagcttag taattctcca
      841 cttctcaaac cgcagctata tattaatgat aacatggctt tgtgtttaat attgctatgt
      901 gcattgagta tctgttttac ttcttctttg cttaaaatat gtggcagttt tctttctctt
      961 tttggtcgat gtattaattc ttcgttcatt tttttatttt caatggttct aaaaaataat
     1021 tttactgcac ttacaatttg gttttgatag gaggaagaga attcattttt aagaatgtaa
     1081 tcattattat agaatatcaa atcatcgttg gtaattgttt caatctgttt agtattaaaa
     1141 tatatcaaaa atgactttag tgcgtcgatg taggttttga tcgtattttc gctatatcgt
     1201 ttggaatgca accatcgact gaattggtct attttggctt gtttatctgc atgtaaaaca
     1261 gcattctcga gcataaatat tttcctattt tcttcattat taggcaaatg ccatgccttt
     1321 agtgtgtagc tccactgtgc atcatcatac ttttttatct gttgaatgag gtttacatca
     1381 tatttgaaat acactgcaat tcgttcttgt ttcttgtatt ttacaatagc tgcattccac
     1441 tccataaata cgctttacta ttaagagtca tgagtgttgg aaattccata atttattata
     1501 aacaattttg ttaaaatcat aaaaataaat tacaacttaa gtaaaacggt gtgtttcgaa
     1561 tgaagagtat gataactgat gattacatta tactagacga tgtctaagac ttcaattatc
     1621 acatcataaa tttgacgaag ttgtgttttt gtaattacat agggtggcat cacatagacc
     1681 gtattgccaa gtggtcgcaa tagaattcct ctgtttatga aattatcata taaaacatcg
     1741 cgaatgttgt tgaaatagct attagaaaaa ttcgatttca cttcgaaggc aatgatggtt
     1801 cctgtttgcc ttatattttc aaccttgtga tgctgtaata aattggcttt catttcactt
     1861 tgccaaacga caagttcatt tatcatatct tgtgcattgc tcaataagtt tagacttgca
     1921 tttgcggcag cgcatgccaa tggatttgcg gtgtatgaat gtccatgtaa aaaagttttg
     1981 agtttattac tgctcaaaaa agcgtcataa attttttgtg tgcaacttgt aatgcctaat
     2041 ggaaggaagc cacctgtaat gccttttgac atacagaaaa tatcagcttt gtgctgcaaa
     2101 taatctgatg caaaattttt gcctgttcga ccaaaaccag tcatcacttc atctgcaata
     2161 caaatgatat tattttgctt tgcaatagct atcagcttat ctaagtatat ggcatcatac
     2221 atcaacattc cggccgaacc ttgaacgagt ggttcaaata taaaggcggc tacttttttt
     2281 gattgtataa tctgtagaaa ttgatttgat aaatgattta tatttgaagc tgtaggcaaa
     2341 tcgataaatt cgacatcaaa taaatatggt tcgaaagctt gattaaaaat accacgtgca
     2401 ccaactgcca tggctccaaa agtgtcgccg tggtatgcgt ttttaaaggc gataattgtg
     2461 tttttttcaa tttgctgatt gtgccaatat tgaattgcca tttttagggc cacttctacg
     2521 ctggtagagc cattatcaga aaagaaaatt ttattttgat tgttaggcaa aattttcaat
     2581 aatgtttctg ccacatcaat ggctggttga tgggtgcacc cagcaaatat aacatgctct
     2641 aattggtcca gctgattttt aattgcattg ctaatttcaa ggttagcatg tccaaatagg
     2701 tttgtccacc aactagaaac agcatcgata tattctttgc cattttcatc atataacgaa
     2761 gcagcattgc cgtgtgtaat gacaatatta ggagcagccg tttggtgttg tgtaaaaggg
     2821 tgccaaatat aatttgtatc tttttcagaa agattcataa tctaaaggta agttggtgtc
     2881 tgtatcttga tttggtaatg gacttaacaa aggcaacaga tttttacgaa ttgaactagc
     2941 ttgatgagca atgaagcctt tcgacaaaat gtcagcttta gctattttac ctaaaagacg
     3001 aacgccagtg tgttgcaaaa taaaagtttc agatgaacta ttttcatcac cattaaaaac
     3061 aatacctgcg atatctatat tatggtactt tagtgattca atagttagca gtgtatgatt
     3121 aatgcttccc aaataattca tagaaaccac tataacagac agttgtaatt gttttattaa
     3181 atcaattatt aaataatttt gagaaagagg aaccatcagt ccaccagctc cttctactat
     3241 caagtaatta tttgattttg gtaatttaat aaattgatta tcaatatttt tattcgataa
     3301 atatgcagct aaatgaggcg aaaccggatc ttcaaataaa taagcttcat catgtatagt
     3361 ttgatggtgc aaggtgtact cctcaataaa gtccgaatcc gtaaaatcta agtttccgca
     3421 ttgaataggt ttccaatagt cagcttgcaa agcctccacg acaatggcag acactactgt
     3481 tttaccaaca tctgtaccga ttcctgtaat aaaaaagcct ttcattaatc agataactgt
     3541 tttataatca agtaataacg aaaataatct atcaatttca tcttttgtat taaaactatg
     3601 caatacaacc ctgagccgct ctctacctaa tggaacggta ggtgaaagta tggctcttac
     3661 atctaactta ttcatctgta aatatcttgc tatctctttg cagtttttat taccaggaac
     3721 aataatacca aaaattggac cttcaccaca cacctcaaac ctactagcaa gcttattaat
     3781 attgtcataa aagtaattat ttaaattatt taatttaaat aattgatttc tattgctttg
     3841 tataaaacta taactatgtt tcacgctcaa tagattatgc gtatctaagg ctgttgtata
     3901 gatgaaattt ttagaaaaat tgattaaata gtcttttaac aactgagtgc ctaagattgc
     3961 agcaccatga cttgcaatcg cctttccgaa agtatagatg cgtgcaaaac acttttcctc
     4021 tactgctgat acattacaaa gtccacttcc atttatgcca aaaagtccgc cagcatgtgc
     4081 ttcgtccacg ataaggttca aacggaattg tgcacatatt tcagccaatt cactcaaatt
     4141 ggctttatcg ccatccatag aaaaaacaga ttctgttatc aggaaagcta tatcatagtc
     4201 gctgttatta agcttttgaa tcaaatcgcc taactgatta tgtttgtaag gtattaattt
     4261 tgcgccactc aaacgcatac cttgatgtat ggagacgtgc accatttcgt cgtagaaaat
     4321 ggtatcttta ggacgaatta cagtagaaat caagccgacg ttagcatcaa aacctgagtt
     4381 aaacaagagc gcagcttctg catggtgaat ctgcgctaaa cgctcttcaa tttcttcaca
     4441 aagtaaagag tttccattta acaagcgaga accagtagcg ccacttttgt ggaattgata
     4501 gtttttaaag tcatcttgta cttgttgtct aataaatgct gaacgcgaca agcctaaata
     4561 atcatttgaa gataaatcaa ttaagtcatt agtaatgatt aacttacgaa aaagatgctc
     4621 attgtttcgt tgaagtaaaa aatggtttat tctttcaaca gcattcatgt aaaagattaa
     4681 atttgttggc taatgattag cgcaacagga agtttctgcg cttacttctt cttgtttgaa
     4741 agcaactcgt ggcctcaaac caagcaattc aaacatagat tgatcgtcag caaagcttgg
     4801 attcggcgtg gttaataatt tatcaccagc aaaaattgaa tttgcacctg caaaaaagca
     4861 taatgcttgc tctgctactg gcatatctaa tcgacctgcg cttaaacgga ctaatgttaa
     4921 aggcatggtt atgcgagccg ttgcaatcat gcgcaccata tcaaacacag aaacgcgctt
     4981 catgtcttcc agtggtgtgc cttcaactgc tactaatgca ttaacaggca cagaatcagg
     5041 atgttgtggc aatgtagcta aagtatatat catacctatt cgtgcgtctt cattttcacc
     5101 taatccgatg atgccaccac tacaaacact aattccagct tttcttacat tgccaattgt
     5161 attcagtctg tcttgatagg ttctggttgt aattacttta tcataatgtt cctctgaagt
     5221 atcgatatta tgattatagg catataatcc agcatttttc aaacgaatag cctgctcctc
     5281 tgtgagcatt cccattgtgc agcaaacttc cattcccaat tgatttacgc cactcaccat
     5341 ttctaaaacc ttatcaaaat ctttgttgtc gcgcacttca cgccaggctg cacccatgca
     5401 aaagcgcgta gagccgtttt cttttgcttc cattgctttg gagattacat cgtcaacatc
     5461 gagtaattta tgagctttta ggccggtatt atatcttgct gcttgtggac aataagcaca
     5521 atcttccgga cagcctccag ttttaacaga aagtaaggtg ttgacttgca cctctccaat
     5581 cctattgaat ttttggtgta ctgctgatgc ctcgtacatc aattctaata atggtttttg
     5641 gtaaatagcc tttatttctt caaaagtcca attgtttctg acttgcataa taatttattc
     5701 gttcagtaaa ttaagttaaa ttatttggaa tccattccat tcttacgttt aaaatttttg
     5761 aaaacaggat tatttttaat gtattcctct cccatcgcct gttcttgttt tttataacta
     5821 gatatatcat taatattagg aggaattacg acgttattat ctattggagc tgttgttgta
     5881 tctaaagctt ttattttata taataagcta tcaacagcca ccaataagct tgaatattct
     5941 ttaggatgtg cctgataata acgcaaagta gcgaaaaatg aagtactatc cactttgtga
     6001 tgttttagaa tatctgggta atatatttta cgtactaaag aaacggaatc cgaatttcgc
     6061 cattttaatt gctcatttac ataagcttcc atcaagaaaa catcggtcaa aactgttgcc
     6121 attttttttt cctcgagtat ctttttatcc gtatcaaatc tatgtttacg aacacatgaa
     6181 ataaatataa acgttgcaat tatgcataac ataaattttg agctatattt ataaaaaaaa
     6241 tcgagtactg tcatgccaac aatttctgta ctgcaagata taacaaaata tgctaaagcc
     6301 aaaaatgctg ttttagttgc tatttctaaa actaaaaccg ttgatgacat tcaacaaata
     6361 tacaatgctg gccaaagagt ttttggggaa aataaagtgc aagaactggt gcaaaaatat
     6421 gaattacttc ctaaagatat acaatggcac atggtgggta atttacaaag taataaagta
     6481 aaatacattg caccatttat ccaattaata cactcagttg atagcttaaa acttgctatt
     6541 gaaatcaata aacaagcact gaaacacaac agagttattg acattctctt agagatacat
     6601 attgctgcag aaacaaataa aagtgggttt tctgaagatg aattaataga tcatttggat
     6661 attaatgcat ttaattcttt gcaaaatata cgaattgttg gcttaatggg aatggcaaca
     6721 aatacaaaaa gtcaggtttt aatcaaaaac gaattcagtg gattaaaaca gtattttgat
     6781 aaaataaaac aacagtattt tagcaagtcc gatgaatttt caatactttc tatgggcatg
     6841 agtggcgatt atcagcttgc attggaagaa ggctcaacga tgataaggat tggatctgca
     6901 atatttggag aacgcgaaat taagaaaaat ccgaatattc ctatttaaag atagtattgg
     6961 caatttgcat ggcgtttata aatgcttctt tatttaaacc atgttcgata ttattttctt
     7021 caaaataagc tactaaattt tccgtaggca tatttcctgt cagtttatct gttgccatag
     7081 gacagccgcc ataccctttt attgcaccat caaatcgagt gcaacctgca ttccaagctg
     7141 cttctacttt ttctcgccat gttgtaggtg ttgtatggaa atgtgctcca aaacctattt
     7201 ctgggaaatc ttctgacaaa atcgaaaaaa cagattgtat taattcaggt gttgcaacac
     7261 ctattgtgtc tgaaatcgaa aacttattta ctccgatttc tagcaatcta tccaccc
//
LOCUS       DAOQQV010000124         7274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4149_length_7274_cov_6.688738, whole genome shotgun sequence.
ACCESSION   DAOQQV010000124 DAOQQV010000000
VERSION     DAOQQV010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_7274_cov_6.688738"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLL59_10770"
     CDS             <1..913
                     /locus_tag="PLL59_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY24332.1"
                     /translation="QAKVYIAATGIWTDDTMKLLRNNSSEQFMMPSKGIHLIVDASHF
                     PKDCVMLLPTANNDGRMIWCMPWDDNLSVVGTTDTEYNGSNEEMTVLKEEVNYILDSV
                     NAQLKDKKLTHDDILSVYAGIRPLLNEKTNNNKSTKRSRDYKIWWNNDNMLTISGGKL
                     TSFLSMAEKVIEEIEQKHSLETSMNNSIKINSIIDKFKHRYGIKNASLIADIAQENIQ
                     FIDTFDNYNYCTAEILFFIRHQHAQKIDDILTRRTLISYQMKNYDTLFIQNISDILAK
                     ELNWSNEDKQMDIENYKKSWMLMHSWL"
     gene            957..1904
                     /locus_tag="PLL59_10775"
     CDS             957..1904
                     /locus_tag="PLL59_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HMY24333.1"
                     /translation="MKLAFLINGTASGVNKLKYKIETTFKEFEPKLFVSESAGHITQL
                     AQTAVARSYDTIIICGGDGTLNEGINGIINALKIGLGDTHDAYDWEKIAQIKIGILPA
                     GSGNDFSKTVMVGKDIDYLKNLILQKRSQLIDIGWVSFFKPNNEPAHRFFINITDVGM
                     GGETVLKLQKNKIPALGASFNYFWAITSTLASYEKVKIKATGDNFLWEGKAINFVVAN
                     GKYFGNGLAVAPDASVSDGKFEIVVIGDVTLLDYFKNLGKVKKGERIIHPEVHYFQLE
                     KVSIESAESRQISIDMDGEFIGYAPMTLINLHHKINFIF"
     gene            complement(1977..3014)
                     /locus_tag="PLL59_10780"
     CDS             complement(1977..3014)
                     /locus_tag="PLL59_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMY24334.1"
                     /translation="MKKVISCLLFITLLFLYDYSKANDAEGSGHDVGFSIGPNFALTD
                     LGGANKIGSPFLRDVDFKATRYCISAFYRYNVNKIFAVRANLMYGMLKADDRNTDGRP
                     PAPGQSPDDSWFRARRNLNFETHMFEFQAIGEVNLKKYIHNEAKGSKERWAPYLGAGL
                     GFFYFNPYTYEQDAAGNKIGSKIKLRPLGTEGQTIGYKKMYSNFQFDLMALLGIKYNV
                     TELFSIALEGVYHQTFTDYLDDVSGSYVNPQDVSSMSPIAQVLQNRVNTGRYPQNEHN
                     YVEGQKDVITGNLKPIGIGQQRGDKKDNDQFFHVQLTFSFSLGATGKRLGFGSCGKRN
                     PYHHKFACPKW"
     gene            complement(3058..3693)
                     /gene="nth"
                     /locus_tag="PLL59_10785"
     CDS             complement(3058..3693)
                     /gene="nth"
                     /locus_tag="PLL59_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HMY24335.1"
                     /translation="MTKKEKADYIVKTLDALYPNPPIPLDHKDAYTLLIAVLLSAQCT
                     DVRVNQVTPALFTLADNPYDMSKIPVEDIKSIIRPCGLSPKKSQAISDLSSILITEYN
                     GNVPSTFTDLERLPGVGHKTASVVMSQAFGVPAFPVDTHIHRLAYRWNLSSGKNVEQT
                     EKDLKRVFREELWNKLHLQIIYYGREYCTARGHNVQYCMICAKIGRKIMDK"
     gene            3743..4219
                     /gene="rnhA"
                     /locus_tag="PLL59_10790"
     CDS             3743..4219
                     /gene="rnhA"
                     /locus_tag="PLL59_10790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HMY24336.1"
                     /translation="MVEIYTDGAARGNPGPGGYGVVMLAAGHRKEISKGYRLTTNNRM
                     ELMAVIVGLEALKKQDLDITIYSDSKYVVESVEQGWVFQWDRKEDFAKKKNRDLWKRF
                     LKLYAYQNIRFVWVKGHSSNIENNRCDILATEAADGFELDVDEAYEREINNKKGMF"
     gene            4222..4965
                     /locus_tag="PLL59_10795"
     CDS             4222..4965
                     /locus_tag="PLL59_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMY24337.1"
                     /translation="MPIEVYIISIIFLGSLIYSTFGFGDALFSMPFLTLIIGIKTATP
                     LMTMNGTTLALVLFIKHRNDIQWRAALKLLLASVCGIPFGIYFLKYGNENIIKIILGL
                     IITSVSVYNLYIKKDAITQIIPSFSVYFFGFIAGILGGAFNTGGPAVAIYGTLSGWTQ
                     LQFVSTLQGYFLPNDVFILIGQLSAGLLNKTVVYYYLICLPFLLLATLIGNKIRKMIP
                     TTKFNRYLFGILMCVGLVFLLRALLQLQA"
     gene            complement(4954..5163)
                     /locus_tag="PLL59_10800"
     CDS             complement(4954..5163)
                     /locus_tag="PLL59_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24338.1"
                     /translation="MKREIKAVKNGEKIKFSLFSPFDKKVSIKVRQQNGIKSIVFHLQ
                     GFLLGEDLNQVEIVENGIKHGALGL"
     gene            5368..6975
                     /locus_tag="PLL59_10805"
     CDS             5368..6975
                     /locus_tag="PLL59_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24339.1"
                     /translation="MPKNDYVYELIQSLNKSEKRYFKLYFATATKTPKHVLLFSILEK
                     ESQYDEKKIRQKLTDEYLKKNFAETKYYLYQQILKVLKDYRTENSVDTILIEKTQNIE
                     ILLQKALFEQAINELEKAKKMAFEHERFEFLTLLLSYQNKIIVSTRLQGYNNIDLENF
                     WLEKVNVFKNFIDTQTARHKVMQLLESKNRIGYIKTNKDKQFYETIKGEIDNLLNNAD
                     CTIAKIYAIESLVFYYNHTDEKKLQNFYCKKLIELHTHNPSINASNPYQLPSAIHNLC
                     LSEINLLNFSEALKWIDIQKKYLEIIHNDIEIKYKVEWSLASLQSYLYNVSGRFQEGK
                     TVVKDIIYWIENTEQIDSRSKWIFYGNCAEIVLGAKDYKQFFYCMRKIREHWVHIDDY
                     NKINYTILQILAHLDEHEIDAVIYLQNALEKEIASVRKGNSTKIETALLQLFQSIKES
                     KKNTSLTKILQAAKQALFENEHEMQMGEFEYALWIEQMLNKNSYENLVRQKRSLQAKD
                     FDQYWNDDYKIQVNINDFRKTLPKRIR"
BASE COUNT         2636 a         1152 c         1160 g         2326 t
ORIGIN      
        1 tcaagcaaag gtatatattg cagcaacagg tatttggacc gatgatacca tgaaattatt
       61 acgcaataat tcttcagaac aatttatgat gccgagcaaa ggaatacacc tgattgtaga
      121 tgcttcgcat tttccaaaag attgtgttat gcttttgcca actgcaaata atgatggtag
      181 aatgatttgg tgcatgcctt gggacgacaa tttaagcgtt gtcggtacta cagatactga
      241 atataatggt tcaaatgaag aaatgacagt actgaaagag gaagttaatt atattttaga
      301 cagcgtaaat gcacaactta aagacaagaa attaacgcac gacgacatat taagtgtgta
      361 tgcaggaatt agacctttat taaacgaaaa aactaacaat aataaaagta caaaacgttc
      421 aagagattat aaaatatggt ggaataatga taacatgctc actatttcag gtggaaagct
      481 gacttctttt ttatcaatgg cggaaaaagt aatagaagaa attgaacaaa aacacagctt
      541 ggaaacatcg atgaataata gtataaaaat aaactctatt atcgacaaat ttaaacatcg
      601 ttatggcatt aaaaatgctt ctttgatagc tgacattgca caagaaaata tacaatttat
      661 cgacacattt gataattaca attattgtac tgctgaaata ttatttttca ttaggcatca
      721 acacgcacag aaaattgatg atatacttac aagaagaacg ctgatttctt atcaaatgaa
      781 aaattatgac acgcttttta ttcaaaacat atcagatatt ttagcaaaag agttaaattg
      841 gagtaatgaa gacaaacaaa tggatataga aaattataaa aaatcatgga tgcttatgca
      901 ttcttggcta taatattttc ttcatttttt tgattgataa ttcttaatac aaaaacatga
      961 aacttgcttt cttaattaat ggtacagcat ctggcgttaa taaacttaaa tataaaattg
     1021 agactacttt caaagaattt gaacccaaac ttttcgtctc agaatcagct ggtcatatta
     1081 cacaactcgc acaaactgct gtagctcgct cgtatgatac aattatcatt tgcggcggtg
     1141 atggcacact taacgaaggc ataaatggaa ttataaatgc attaaaaata ggattaggcg
     1201 acacacatga cgcttatgat tgggaaaaaa ttgctcaaat aaaaattgga attttacctg
     1261 ctggaagtgg taatgatttt tcaaaaacag tgatggtcgg caaagatatt gactatctta
     1321 aaaatctaat tttacaaaag cgctcacaac tgatagacat cggttgggtt tcctttttca
     1381 aacctaataa tgaaccagct catcgtttct tcattaatat tacagatgta ggaatgggcg
     1441 gcgaaactgt tttaaaactt caaaaaaata aaattcctgc tttaggtgca agcttcaatt
     1501 atttttgggc aattacttca acgcttgctt cttatgaaaa agtaaaaata aaagccacag
     1561 gcgacaattt tttatgggaa ggaaaagcca ttaattttgt agtggctaat ggtaagtatt
     1621 ttggaaatgg tctcgccgtt gcgcctgatg catcagtatc agatggcaaa tttgaaattg
     1681 tagtaattgg cgacgttact cttttagatt actttaaaaa tttaggaaaa gtaaaaaaag
     1741 gagaaagaat aattcatcca gaagtgcatt atttccaatt agaaaaagtc agcatagaat
     1801 cagcagaatc aagacaaatt tctattgata tggatggcga atttattgga tatgcaccaa
     1861 tgacacttat caacttgcat cataaaataa atttcatttt ttaatttaat agcctccgta
     1921 aaataaaaat gtcctgaatt ttttcaggac atttttcata aattatgaaa tgcattttac
     1981 cattttggac aagcaaattt atgatgatat ggattacgtt ttccgcagct accaaatcct
     2041 aatcgtttac cagttgcacc taaagagaat gaaaatgtta actgaacatg gaaaaattga
     2101 tcattgtcct ttttatcacc acgttgttgc ccaattccaa taggttttaa gttacctgta
     2161 atcacatctt tctgaccttc tacatagtta tgctcgtttt gaggatatct acctgtattt
     2221 actctatttt gcaatacttg tgctattggc gacatagaag aaacatcttg tggattaaca
     2281 tagctaccac ttacatcatc taaatagtct gtaaatgttt ggtgataaac accttctaaa
     2341 gcaatagaaa ataattctgt tacattatat ttaattccta ataatgccat caagtcaaac
     2401 tggaaattgc tatacatttt tttataacca atggtctgac cttctgttcc taatggtctc
     2461 aattttattt tagaaccaat tttattacct gcagcatctt gctcatatgt gtaaggattg
     2521 aaataaaaga aacctaaacc tgctcctaaa tatggtgccc atctttcttt tgagcctttt
     2581 gcttcattgt gaatatattt tttcaaattc acttcaccaa tcgcttgaaa ctcgaacatg
     2641 tgtgtttcaa aattcaaatt acgtcttgca cggaaccagc tatcatcagg gctttgacct
     2701 ggtgctggag gtctgccgtc tgtatttctg tcatcagcct ttaacatacc atacatcaaa
     2761 ttagcacgaa cagcaaatat tttattgaca ttatatctat aaaatgcaga aatgcaataa
     2821 cgagttgctt taaaatcgac atctcgtaaa aatggtgaac caattttatt agcgccgcct
     2881 aagtccgtta aagcaaaatt tggtccaata gaaaaaccta catcgtggcc acttccttca
     2941 gcatcattcg cttttgagta atcgtataaa aacagcaagg taataaacag taaacatgaa
     3001 atgactttct tcatcgggtt agttttagtt attatcggta aaaataccaa aaaaaaatta
     3061 tttatccatt atttttcttc caattttagc acaaatcata caatactgaa cattatggcc
     3121 tcgtgctgta caatactcgc gaccataata aataatttgc aagtgtaatt tgttccatag
     3181 ctcctcacga aatacacgtt ttaaatcctt ttctgtttgc tccacatttt ttccagaact
     3241 caaattccat ctgtatgcca atctatgtat atgtgtgtca acaggaaaag caggcactcc
     3301 aaatgcttga ctcatgacaa cacttgccgt tttatgacct acgcctggaa gtcgctctaa
     3361 atcagtaaac gttgaaggca catttccatt gtattctgta attaaaatac ttgacaaatc
     3421 agatatcgct tgtgattttt ttggagacaa gccacatgga cgaatgattg atttgatatc
     3481 ttctactgga attttcgaca tatcatacgg attatcagcc aaggtaaaca aagcaggtgt
     3541 cacctgattc actcttacat ctgtgcattg agcagataat aaaacggcaa ttaatagcgt
     3601 atatgcatct ttatgatcta atggaatcgg cggattcgga tacaatgcat caagggtttt
     3661 tactatataa tctgcttttt cttttttggt cacgttcaaa aataagaatt agaatagtat
     3721 attcgattcc aaaaattcca caatggttga aatctataca gacggtgcag caagaggcaa
     3781 tcctggtcca ggtggctatg gcgtagtcat gctggctgct ggccatagaa aagaaatttc
     3841 caaaggctat cgtttaacta caaacaacag aatggaatta atggctgtta tcgttggatt
     3901 agaagcattg aaaaaacaag atttagatat tacaatttat tccgattcaa aatatgtggt
     3961 agaatctgtt gaacaaggat gggtttttca gtgggacaga aaagaagatt ttgccaagaa
     4021 aaaaaatcga gatttatgga aacggtttct gaaattgtat gcataccaaa acattcgatt
     4081 tgtttgggta aaaggtcact ctagcaatat agaaaacaat aggtgcgata tcttggcaac
     4141 agaggctgca gatggttttg aactcgatgt tgatgaagct tatgaaagag aaattaataa
     4201 caaaaaagga atgttttagc agtgccaatt gaagtttata tcatttctat tattttttta
     4261 ggttcgctaa tatatagtac gtttggtttt ggtgacgcgc ttttcagtat gccttttcta
     4321 acattaatta ttggcataaa aacggcaaca ccattaatga caatgaatgg cactacttta
     4381 gcacttgtat tatttataaa acacagaaat gacattcagt ggcgtgctgc cttgaagtta
     4441 ttgctcgcct ctgtttgtgg gattccgttc ggcatttatt ttttaaaata tggcaatgaa
     4501 aatatcataa agattattct aggccttatt attactagtg tttctgtata taatttgtat
     4561 ataaaaaaag atgccattac acaaataatt ccatcatttt ctgtatattt ttttggattt
     4621 atagcaggca ttttaggtgg cgcatttaat acaggtggtc cagctgtggc tatctacgga
     4681 actttaagtg gctggacaca attacaattt gtgagcacgt tacaaggcta ctttttgcca
     4741 aatgatgttt ttattttaat tgggcaatta agcgcaggat tattaaacaa aacagtagtg
     4801 tattattact taatttgttt gcctttttta ttattagcaa cacttattgg aaataaaatt
     4861 cgcaagatga taccaacaac aaaattcaat cgttatttat ttggtatttt aatgtgtgtt
     4921 ggcctagtat ttcttttgcg agcattgttg caattacaag cctaaagcac catgcttaat
     4981 gccattttct acaatttcga cttggtttaa gtcttcgccc aacaaaaagc cttgcaaatg
     5041 aaaaacaata cttttgatgc cattttgttg acgcacttta atgctcacct ttttatcaaa
     5101 tggcgaaaac aaggaaaatt ttattttttc gccatttttc actgctttaa tttctcgttt
     5161 cataaactca gattcaacac gaatcgtttc tattgaataa cctgaatgat tgtaaattac
     5221 cattttgtga ctggcatata gataagcacc aatcaagatc attaaaacaa aaagaaaaat
     5281 ataagtagag cgtgttcgca taagacaaat tagcttacaa aattattaaa ataattataa
     5341 tttagcaaat ggaaacacca cagcaatatg ccaaaaaatg attatgtata tgaattaatt
     5401 cagtctttaa ataaatctga aaaaagatat tttaaactct attttgccac agcaactaaa
     5461 acgcctaaac atgttttatt attctccatc ttagaaaaag aatcgcaata tgatgaaaag
     5521 aaaataaggc aaaagttgac tgatgaatat ttaaaaaaga attttgcaga aacaaaatat
     5581 tatctatacc aacaaattct aaaagtatta aaagattata ggacagaaaa ttctgttgac
     5641 actatactca tcgaaaagac gcaaaatatt gaaatcttac tgcaaaaagc tttatttgaa
     5701 caagctataa atgagttgga aaaagcaaaa aaaatggctt ttgaacatga gcgatttgaa
     5761 tttttaacct tgcttctctc ctatcaaaat aaaattattg tatccaccag attgcaagga
     5821 tacaacaata tcgatttaga aaatttttgg ctggaaaaag taaatgtatt taagaatttt
     5881 atagacacgc aaactgctcg acataaagtc atgcaattat tagaatccaa aaacagaatc
     5941 ggatatatta aaactaacaa agacaaacaa ttctacgaaa ctataaaagg tgaaatcgat
     6001 aatttgttaa acaatgctga ctgtacaatt gctaaaatct atgcaataga aagtttagta
     6061 ttctattaca atcatacaga tgaaaagaaa ttacaaaatt tttattgcaa aaaactgatt
     6121 gaattacaca cacataatcc atcaataaat gcaagcaatc cttaccaatt gccatctgcc
     6181 atacataatt tatgtttgag cgaaattaat ttattaaatt tctcagaagc tttaaaatgg
     6241 attgacattc aaaaaaaata tttagaaata atacacaacg atattgaaat aaaatataaa
     6301 gtagaatgga gtttggcgtc gctacaatcc tacttatata atgtttctgg ccgtttccaa
     6361 gaaggtaaaa ctgtcgtaaa agacattatt tattggatag aaaacacaga gcaaatcgac
     6421 tcacgcagta aatggatttt ttatggcaac tgtgctgaaa ttgttttagg tgctaaagac
     6481 tacaagcaat ttttttattg tatgcgcaaa atacgtgaac attgggtgca catcgatgat
     6541 tacaataaaa taaattatac aatattacaa attttagcgc acttagatga acatgaaatt
     6601 gacgctgtca tctatttaca aaatgcactt gaaaaagaaa ttgccagtgt acgcaaagga
     6661 aattctacaa aaattgaaac cgcattattg caattattcc aatcaatcaa agaatctaaa
     6721 aaaaacactt ctttaacaaa aatattacaa gcagcaaaac aagcattatt tgaaaatgaa
     6781 catgaaatgc aaatgggaga atttgaatat gcgctttgga tagaacaaat gctcaacaaa
     6841 aattcgtatg aaaatttagt aagacaaaaa cgcagcctgc aagccaaaga cttcgatcaa
     6901 tattggaatg atgattacaa gatacaagta aatatcaacg attttcgtaa aactttgcca
     6961 aaacgtattc gataaaaatg ttagagacga cattgctgtc gtctctataa taaattaact
     7021 aaccagtctt tatgaaataa aatcaagtag ttgtttcaca acagcttttc atagttttat
     7081 gatttcattc acctttcaaa actatatctt aataacataa agtaatatta tagttagtat
     7141 ttggacgatt tgaatgagta aacggtaaaa aattgtttgt ttttataata ttctttacat
     7201 ttgagcatga acaacactag taatttcaag aaaggatttc attctatttg tgtgaaagat
     7261 cctttaattg atgg
//
LOCUS       DAOQQV010000125         7140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4278_length_7140_cov_8.055469, whole genome shotgun sequence.
ACCESSION   DAOQQV010000125 DAOQQV010000000
VERSION     DAOQQV010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4278_length_7140_cov_8.055469"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..335
                     /locus_tag="PLL59_10810"
     CDS             45..335
                     /locus_tag="PLL59_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24340.1"
                     /translation="MQKQSTFYAVVSIFFFWGFMAASNGIFIPFCKEHFHLSQFQSQL
                     IDSTFYGGYFIGGIIFAGPQIGVKYFFNPKIGVYGEIGYGKSVFEAGVSIKL"
     gene            complement(1100..1534)
                     /locus_tag="PLL59_10815"
     CDS             complement(1100..1534)
                     /locus_tag="PLL59_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMY24341.1"
                     /translation="MAFVSIYLNYMGNAEEAFEHYKKVFNTEYSTPLMRIGDMPDQEG
                     MPPLSDEDKKKVMHVAITVYGTQLMATDMLESMGHQLILGNNITISLNPDSKAEADRL
                     FTALNNGSKNAVAPHDEFWGYWGCCTDKFGVQWMFNVSNIEK"
     gene            1701..1994
                     /locus_tag="PLL59_10820"
     CDS             1701..1994
                     /locus_tag="PLL59_10820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY24342.1"
                     /translation="MEIQHQKQNNQGVFFIANEDGEHLASMTYIMDTPTIMRIEHTTV
                     SEQLAGQGVGKKLVEAGVNFAREHQYKIIPACSYARALIEKTETYHDVWQRDV"
     gene            2068..2364
                     /locus_tag="PLL59_10825"
     CDS             2068..2364
                     /locus_tag="PLL59_10825"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HMY24343.1"
                     /translation="MIVYTLTANVDEDIADEWLQWMKNEVLHFALGTQLPVEFKSFKV
                     LNDNEGVTYTFQLFFKDVFVYKLFLTEHHADFSKLINAKYTDKAVYFNTLLQQL"
     gene            2665..5217
                     /locus_tag="PLL59_10830"
     CDS             2665..5217
                     /locus_tag="PLL59_10830"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HMY24344.1"
                     /translation="MKTTASEISTTTFTQEEAFAASLKYFNGDDLAARVWLNKYALKD
                     SDGNIYEQTPNDMHHRLANELARIEKRYPNPMSAEFIFDLIKNFKYIIPQGSPMTGIG
                     NPYQIASLSNCFVIGNEGDSDSYGGIMKVDEEQVQLMKRRGGVGHDLSHIRPKGSAVK
                     NSALTSTGLVPFMERYSNSTREVAQDGRRGALMLSVSIAHPDAEDFIDAKLEQGKVTG
                     ANISVRINNAFMQAVENQTTFTQQFPIFSKTPLFKKEIDALALWKKIVHNAWRSAEPG
                     ILFWDTIINESLPDCYADLGFRTVSTNPCGEIPLCPYDSCRLLAINLFSYVDHPFTKN
                     ATFNWNLFKQHISYAQRMMDDIIDLELEKIDVILEKLNQDPEGKEVKRTEINLWEKIK
                     EKAKQGRRTGIGITAEGDMLAALGLRYGSDEGIAFAVNIHKTVALEAYRSSVHTAKER
                     GAFEVFDAEREKENPFMLRLKEADSQLYYEMLEYGRRNIALLTIAPTGTTSLMSQTSS
                     GIEPVFLPVYKRRRKVNPNDKNVRVDFVDEVGDSWEEYVVFHHRFKQWMDINGYNSNR
                     NFSQDELDELVKKSPYYKATSNDVDYLKKVQMQGAIQKWVDHSISVTINMPNDVTEET
                     VAECYMEAWKSGCKGVTVYRDGSRSGVLVSTDEKKKESDADDGSTFPLTRPISLKADV
                     VRFQNNKDKWIAFIGLIDNKPYEIFTGLSDDEDGILLPRWVNDGVIIKNKETDGTSRY
                     DFQYKNQRGYKTTIEGLSYKFNPEYWNYAKLISGTLRHGMPIENVVDLIGSLDLDEAI
                     NTWKNGVARALKRYVPDGTLSNKSKCPNCGSTNQQYLEGCLTCKDCGASRCG"
     gene            5344..6108
                     /locus_tag="PLL59_10835"
     CDS             5344..6108
                     /locus_tag="PLL59_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HMY24345.1"
                     /translation="MKFLFIIVFIFQFNCILAQELMAEMESIATDRPDQTETPAVLPF
                     KFFQLETGFNIESQTNVYTFVHPTMLWKVGIFKSTELRLITEISTTKDTVDKYRTGLS
                     PIEIGFKTAICEARKARPKISFIAHLAIPYLSTKNRRTAYFAPNFRFTLEHTLKKNIS
                     LGYNLGMEWTGESPHPSFIYTIVNGFDVSEKVYLYYEFFGDIPIKEISTHTFDAGLAY
                     LIKQNQQIDISAGFQLYPFVKGWYVSLGYSFRIPKQ"
     gene            complement(6113..>7140)
                     /locus_tag="PLL59_10840"
     CDS             complement(6113..>7140)
                     /locus_tag="PLL59_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24346.1"
                     /translation="FKAAYLGTNNQNKSSNIDFDNLKSNIDIMKPSSNSSSTTTKTGN
                     YQTALKALNDYLKTFDGDRYQGIEVKDGYVYSRYKNNEFSKAKIDDLDKVVLGAQYSV
                     VKLMCKNESKCIYSTITKGYHDYFNFQTASGKSIYKMEELLKDFLAALQNKTNTTSTT
                     KTTNTTNSAKTIAEIKREQKEKERQNNSSYSSVFEDDVFGYLFDTPITTSNTSTTSTT
                     SKTPDASKYSSKLKALNDYLKIFNPATYKNVNVSNGKVNFDFKIFSKTYTSYIPIDDL
                     VNKTNVVIAKSLTSNEVKIMCKNESDCFYSTYSNDNTDHFRFYSNNIADMTKMKQLVD
                     DFIKSLK"
BASE COUNT         2290 a         1105 c         1297 g         2448 t
ORIGIN      
        1 tttgtttcag aattgttaac atcaattctg aaaataagta acacatgcaa aagcaatcta
       61 ccttctacgc tgttgtgtcc atctttttct tttggggctt tatggccgcc agcaacggta
      121 tttttattcc attttgtaaa gagcatttcc acctgtcgca gtttcaaagc cagttgattg
      181 attctacttt ttatggcggc tactttattg gcggaattat ctttgcagga ccacaaatcg
      241 gtgtgaaata tttcttcaac cctaaaatcg gtgtgtacgg agaaattgga tacggcaaga
      301 gcgtatttga agccggtgtt tccatcaagc tgtaaacatc acttgaaaat ataaacgaaa
      361 aaagtcccga ttttcgggac ttttttcatt tatgcttatc agatataatg ctgacggatg
      421 atataatctg ctttccattg tttattttta ccattgatat aatttagcgc ataacatgaa
      481 ttacggcaaa caacttttag gcatcctttt attgttttca gtgtattctg ctctcggaga
      541 agaagctgta atctgcatga cgaaggacag tattaaagtg aagaataagt caggaagagc
      601 atccacggaa ggagagaatg cgtctgaaaa aggaaatttt gtctttcaac caacgttgaa
      661 tttcggacat catagcggat ttaaaaaggt gtacagcgga ttcggttatg attatgccta
      721 tacgggctta attcccggtg taacagtgaa tctggattat aacgtccata aatacgtatc
      781 cgttggtgtt gcctattctg tggcgtttca gaagtttacc aaatcaaatg tcttttacct
      841 ggaacacgcc tttggtgcga gaagtagttt tcattggtgg caaatgctgg cagataagtc
      901 tgacggtaga ttgctggcgg ataaaattga ttttgacata catgttcatg tcggtggttt
      961 cctgcttacg gaaaaaaata tcaataccgg aaaaaagttg aaacagctgg gtgtaaacgc
     1021 acgatatact ttctcaggtg atgttgcgag aattcgatgt aaagtgacgc tattgttttt
     1081 cataaatata tattacaaat tacttttcaa tattgcttac attaaacatc cattgtacac
     1141 caaatttatc ggtgcaacaa ccccaatagc cccaaaattc atcgtgaggt gcaaccgcat
     1201 ttttactgcc attattaagc gctgtaaata gcctgtctgc ttcggctttg ctatcaggat
     1261 ttaagcttat tgttatatta tttccaagta ttagttgatg acccatgctt tctaacatat
     1321 ccgttgccat taattgtgtg ccatatacag taattgctac atgcatcact tttttcttgt
     1381 cttcatcact taaaggtggc ataccttctt ggtcaggcat atcaccaata cgcatcaacg
     1441 gtgtggaata ttctgtgtta aatacttttt tataatgctc gaatgcttct tctgcattac
     1501 ccatataatt caaatagatt gatacaaatg ccatgttatt tttttattta ttaaaagtaa
     1561 tacatcgaaa tgaataattt tacatttttt agacttgtct aaaaattatt tttattctgt
     1621 ttaaataata gcatttcaat actttttatc tcttttataa tgcaatagct ttttatgctg
     1681 taaagtgtta tttttataca atggaaattc aacaccaaaa acaaaataat caaggcgttt
     1741 tttttattgc taatgaagat ggagaacact tagcttcaat gacctacata atggatacac
     1801 caactattat gcggattgaa catacgacag taagtgagca attagctgga caaggcgtag
     1861 gaaaaaaatt agtagaggca ggtgtaaatt ttgcacgtga acatcaatat aaaattattc
     1921 cagcatgttc ttatgcacgc gctttaatcg aaaaaacaga aacgtatcac gatgtttggc
     1981 agcgtgatgt ataatgaaaa tctatttgaa attggaggaa taaattctct tttataaaag
     2041 aagtatcttt gtgtcataat ttaatacatg atagtttata cgcttaccgc aaatgttgat
     2101 gaggatattg ctgatgaatg gttgcaatgg atgaaaaatg aagtattgca ttttgcatta
     2161 ggcacacaat tgccagtaga atttaaaagt tttaaagtat tgaacgataa tgaaggcgtt
     2221 acttatactt ttcaactttt tttcaaagat gtttttgttt acaaattatt tcttacagaa
     2281 catcatgctg actttagtaa gttgataaac gctaagtaca cggataaagc agtttatttt
     2341 aatacacttt tacagcaatt ataattacag cattttaata cttctttttt ttgttttcat
     2401 ttttttatct aatactgaaa attgtttgac acatcaagaa gtccatattg taatactata
     2461 ctaatttcaa aattattttt actgacacaa ttctgcacaa cggttgataa gtttttgaaa
     2521 acttttttgt gttgcgaaaa aatcaactat ttgaaaatca ttgttttata attattatcg
     2581 caaaactata ctaattttag gtattgctta ggtttttctt tattgtaatt ttgttccatc
     2641 actaacccaa aaaacaacca taaaatgaaa actacagcaa gcgaaatttc gactacaact
     2701 tttacgcaag aagaagcatt tgcagcctct cttaaatatt ttaatggcga tgatttggcc
     2761 gccagagtgt ggctaaataa atatgcatta aaagattctg atggaaatat ctacgaacaa
     2821 acacccaatg atatgcatca tagattggca aatgagttgg ctcgtatcga aaagcgctat
     2881 ccaaatccaa tgtctgctga gtttattttt gacctaatca aaaattttaa atatatcata
     2941 ccgcaaggaa gtccgatgac gggaattggc aatccttacc aaattgcgtc attatcgaat
     3001 tgttttgtaa ttggcaatga aggcgattct gattcgtatg gtggtattat gaaagtagat
     3061 gaagagcaag tgcaattaat gaaacgtcgt ggtggtgtag ggcatgattt atcgcatata
     3121 cgacctaaag gttctgccgt aaaaaattca gcgctgactt ctacaggctt agtaccattt
     3181 atggagcgct attccaattc aacgcgcgaa gtagcacaag atggtagaag aggagcatta
     3241 atgctctctg tttctatagc acatccagat gcagaagatt ttatagatgc aaaattagaa
     3301 caaggcaaag taacaggtgc caatatttct gttcgcatca ataacgcttt tatgcaagca
     3361 gtagaaaatc aaactacttt tacgcaacag tttccgatat ttagcaaaac accattattc
     3421 aaaaaagaga ttgacgcatt agcattatgg aaaaaaatag tgcataatgc ttggcgctct
     3481 gcagagcctg gtatattatt ttgggacact attattaatg agtctttgcc cgattgttac
     3541 gctgatttag gctttagaac agtttctaca aatccgtgcg gagaaattcc tttgtgtcct
     3601 tatgattcct gtcgtttatt ggccattaat ttattttcgt atgtcgatca tccatttacg
     3661 aaaaatgcta cgtttaattg gaatttattc aagcagcata tctcatatgc gcaacgcatg
     3721 atggacgata ttattgattt agaattagaa aaaattgatg taattctaga gaaattaaat
     3781 caagatccgg aaggcaaaga agtgaaaaga acagaaatca atctttggga aaaaataaaa
     3841 gaaaaagcaa aacaaggccg tagaacaggt attggcataa cagcagaagg cgatatgttg
     3901 gctgcattag gtttgcgata tggtagtgat gaaggaatag cgtttgccgt aaacatacat
     3961 aaaacagttg ctttagaagc ttatcgctca tcagtgcata ctgctaaaga gcgtggtgca
     4021 tttgaagtat ttgatgctga gcgcgagaaa gaaaatccat ttatgttgcg tttaaaagaa
     4081 gccgactcac aattgtatta tgaaatgttg gaatatggaa gacgcaatat tgcattattg
     4141 actattgcac ctacaggtac aacaagcttg atgtcgcaaa cgtcttctgg tattgagcct
     4201 gtatttttgc ctgtatataa acgcagacga aaagtaaatc cgaatgacaa aaatgtacgc
     4261 gtagattttg ttgacgaagt aggcgattct tgggaagaat atgttgtgtt ccaccaccgt
     4321 tttaaacaat ggatggacat taatggatat aattctaatc gtaatttttc gcaagatgaa
     4381 ttagatgaat tagtaaaaaa atcaccatat tataaagcaa catctaatga tgttgattat
     4441 ctgaaaaaag tacaaatgca aggtgctatt caaaaatggg tagaccattc tataagtgta
     4501 acgataaata tgccgaatga tgttacggaa gaaacagtag cagaatgcta tatggaagcg
     4561 tggaaatctg gttgcaaagg tgttactgtt tatcgcgatg gctcgcgctc aggcgtgttg
     4621 gtcagtactg acgaaaagaa aaaagaatct gatgctgatg atggttctac atttccactt
     4681 acacgtccaa tttctttaaa agccgatgtc gtgcgttttc aaaacaataa agacaaatgg
     4741 attgcattta ttggtttaat tgataacaaa ccttatgaaa tttttacagg cttgagtgat
     4801 gatgaagacg gaatattatt gccacgttgg gtcaatgatg gtgtgattat aaaaaataaa
     4861 gaaactgacg gcacatcgcg ctatgatttt caatacaaaa atcagcgtgg ttacaaaaca
     4921 acgattgaag gtttgtctta taaatttaat cctgaatatt ggaattatgc aaaactgatt
     4981 tcaggcactc tgcgtcatgg tatgcctata gaaaatgttg ttgatttaat aggtagtcta
     5041 gatttagatg aggcaataaa cacttggaaa aatggtgttg cgcgtgcatt aaaacgatac
     5101 gtgccagatg gtactttatc taataaatca aaatgtccaa attgtggttc aacgaatcag
     5161 caatatttag aaggttgcct gacatgcaaa gattgtggcg cctccagatg tggttaatca
     5221 attttttaag cgatagtaaa cgcctgatat ttcaggcgtt tttttttgtt tatgttataa
     5281 ataatttagt tcggtctaaa aataaaattc gttgctatta aattacgatt ttatttttgc
     5341 caaatgaaat ttctttttat tatcgtattt atttttcaat ttaattgcat attggcacaa
     5401 gaactaatgg ctgaaatgga atcgattgct acggacagac cagaccaaac ggaaacgcca
     5461 gctgttttgc cgtttaaatt ttttcaatta gaaactggtt ttaatataga aagccaaact
     5521 aatgtatata cttttgttca tccaacaatg ctttggaaag taggcatttt taaatcaacg
     5581 gaactgcgat taattacaga aatttctact actaaagata ctgtagataa atatagaaca
     5641 ggcttgtcac ctatagaaat tggatttaaa acagcaattt gtgaagcaag aaaagcaaga
     5701 cctaaaattt catttattgc acatttagca ataccatatt tgtcgacaaa aaacagacgt
     5761 acagcatatt ttgcgcccaa ttttagattt acgttggagc acactttgaa gaaaaatatt
     5821 tcattaggat ataatcttgg aatggaatgg acaggcgaat cgccgcatcc ttcctttata
     5881 tacactattg taaatggctt tgatgtaagc gagaaagtat atttatatta tgagtttttt
     5941 ggcgatattc cgattaaaga aatatctacg catacatttg acgcaggtct tgcctattta
     6001 ataaaacaaa accagcaaat agatatttct gctggttttc aattgtatcc ttttgttaaa
     6061 ggctggtatg tgtctttagg ttattctttc agaataccaa agcaatgaat gtttatttca
     6121 atgacttgat gaaatcatcg accagttgtt tcatttttgt catgtcagca atgttattgc
     6181 tataaaaacg gaaatgatct gtattgtcat tgctataagt agaataaaag caatcgcttt
     6241 catttttaca cattattttt acttcatttg atgtcaaaga ttttgctatt actacatttg
     6301 ttttatttac taaatcatca atgggaatgt aagaagtata tgtctttgaa aagattttga
     6361 aatcaaaatt tactttacca ttgcttacat ttacgttttt gtaagtagct ggattaaata
     6421 ttttcaaata atcgtttaat gcttttaatt tactactgta tttacttgca tctggtgttt
     6481 tacttgttgt tgatgttgtt gaggtgtttg atgttgttat tggtgtatca aaaagatagc
     6541 caaaaacatc atcttcaaaa acggaggaat atgatgagtt attttgacgt tctttttctt
     6601 tttgttcacg cttgatttct gcaattgttt ttgcagagtt tgtcgtattt gttgtttttg
     6661 tggtgcttgt tgtatttgtt ttgttttgca atgcagctaa aaagtctttt agcaattctt
     6721 ccattttata aatgcttttt ccgctagcag tttgaaagtt gaaataatcg tgatatcctt
     6781 ttgtgatggt agagtaaata catttgcttt cattcttaca cattagcttt actactgaat
     6841 attgagcgcc caaaactact ttatctaagt cgtcaatttt tgcttttgaa aattcattgt
     6901 ttttgtatct actatataca taaccgtctt ttacttctat tccttgatat ctatcaccat
     6961 cgaatgtttt caagtaatca tttaacgctt ttaatgcggt ttgataattg ccagttttcg
     7021 ttgttgttga gctgctgtta ctgcttggct tcataatatc aatatttgat ttcaagttat
     7081 caaaatcaat attagaagat ttgttttgat tattagttcc taaataagct gctttgaaat
//
LOCUS       DAOQQV010000126         7107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4322_length_7107_cov_7.073880, whole genome shotgun sequence.
ACCESSION   DAOQQV010000126 DAOQQV010000000
VERSION     DAOQQV010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4322_length_7107_cov_7.073880"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLL59_10845"
     CDS             <1..355
                     /locus_tag="PLL59_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMY24347.1"
                     /translation="TTLADSLIATGFPYYDFEHLDNYLSVFKYLMTSTRGIRRLGSAA
                     IDLCFTACGRFDAFFEYSLSPWDVAAGAIILQEAGGKVSDFDGGNNWLHGRQMLASNA
                     LLSADLLQVIKARYK"
     gene            complement(442..1401)
                     /locus_tag="PLL59_10850"
     CDS             complement(442..1401)
                     /locus_tag="PLL59_10850"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HMY24348.1"
                     /translation="MKKLMLISTAVALLFSACQKGNLMESDDNAMATHRNCNSMEVLA
                     EQLKADPTLEQRMNAYESIVQQNIKENKSARLVNGVIEIPVVVNVLYRTTSENVSTAQ
                     IQSQIDVLNEDFNATNADFSLVPSTFSAVKANVGIRFVLDQVVRKKTTKKSWSTNDAM
                     KKSSQGGINPTSPTTKLNLWVCTLSGGILGYAQFPGGSSSTDGVVILNKGFGRTGTAA
                     APFNKGRTATHEVGHWLNLRHIWGDATCGSDLVSDTPTHNTANYGCPAAGHKSTCSGT
                     PIEMTMNFMDYTDDACMYMFSSGQKARILATFASGGPRASFAQ"
     gene            complement(1519..2415)
                     /locus_tag="PLL59_10855"
     CDS             complement(1519..2415)
                     /locus_tag="PLL59_10855"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HMY24349.1"
                     /translation="MAINTISVIGSGTMGNGIAHVCAQFGFKVALIDISQLALDKAIQ
                     TIQKNLDRQVQKGVLSEELKIQTLANITTYTSIKDCAHASDLVIEAASENFSIKEKIF
                     RELDEICAPHCILASNTSSISITRIAACTKRPDKVIGMHFMNPVPIMKLVEVIRGYAT
                     SNETNQHVFDVSKQLQKIPVEVNDYPGFVANRILMPMINEAIYSLYEGVACISEIDTV
                     MKLGMAHPMGPLQLADFIGLDVCLAIMRVLHEGFGNPKYAPCPLLVNMVTAGYLGAKS
                     GEGFYKYEAGSKELVVSPMFQK"
     gene            complement(2417..2905)
                     /locus_tag="PLL59_10860"
     CDS             complement(2417..2905)
                     /locus_tag="PLL59_10860"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HMY24350.1"
                     /translation="MNAKKDEYKNMVLSPVFRAGLLKVMPMGAIAGLKVTALDDQHCK
                     VTVPYNYVNKNPFNTTYWAVLGMAAEMASGAMVQMHTHKTNPSVSMFVTACSGKFVKR
                     AIGVTTFICNDGQLIADKVHAAMTTMEPQTIQSNVKGYDKAGDLLVDFDFEWSIKARK
                     SK"
     gene            3012..4934
                     /locus_tag="PLL59_10865"
     CDS             3012..4934
                     /locus_tag="PLL59_10865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMY24351.1"
                     /translation="MQKPLHALSNKWTFLLLFILSFVLYGNTIFNDYGYDDVFVVKGN
                     KYVQEGIAGIPAIFENPYAVVDSVQLDKRPITLITFAVEHQLFNDNPHISHFINIILY
                     ALTLLVLYVLLQQAFQLEKVNKWLPLLVVVLFAIHPLHTEVVSSLKNRDELLVMLFGL
                     LYLLLSYKYAHASSRRVLYLSLAIFSLALMLLSKITGFVFVFIGIAMFVFYKWHSINK
                     RNWFFVLWSVLLLFRGLYVTLEGVNREFLVFENPLIKETNLLVKIGTAFLILLHHIKM
                     LIFPNPMLFYYGGNTFPLVTLGNPLALFSMLLHVTFFVYGCVKFFKREMIGLFIICYF
                     ISIMLYANFPIPYTSMYAERTQFISSLWFILILVSLFNLLSSLKPIKGHFGLNNKFVL
                     IVLSVVFIVYSFLTINRNFYWKNSLILMEKDMPYLENSIIANYIYANNLKYESKQAID
                     TSTSKLLAQKAVTHYRQVIKLVPGFPEFHYKLASTYRYNLKNLDSAEIYYGYALSLDS
                     IYADANYEMAKLFFDKADFQRSNYYFEKTYPLKPTDSMTLFYYGQTALKMGNLSTAYK
                     IDKEFLNLYPNHYYPYVNLGVYYSTALKDDSAVIYFDKAIEKGYREPSLLAKLAYYYE
                     TKGNQEKAKYYRSLSR"
     gene            complement(4931..6064)
                     /locus_tag="PLL59_10870"
     CDS             complement(4931..6064)
                     /locus_tag="PLL59_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24352.1"
                     /translation="MKIIGTKAIALLISLIYLLLLTGHAYATRPDSLKVLLFLIFLIH
                     FIDYFYENGSVKSKYVSLLAALFAVATKQDVIIYIALIIGVTFLIQRNKSIILYGISF
                     VILAVFLFISFHIFFGNYCIESLFLYNLQKIKNYRESYNFMIVLFNTVRLSPFYMLFI
                     FLLFNSHKTTQKKLGLLLGISGIFAAVLSSIFLFRPGSYLNYTYELILISILALIYFL
                     KNYPLKKTIIAADFYILFLLASNAVLNNYSINIKKEKKEKQKFQSNYQLRDALQTYLK
                     TNSTIYDVTLQHSLFIADKRVIYGHEIHLDRLIYALLKLKSNSELYNAPTQKYDTYFA
                     DGRVQYILTTNKVSEQQIVSSFYPNYKEEKKINNFILYKFEKP"
     gene            6525..>7107
                     /locus_tag="PLL59_10875"
     CDS             6525..>7107
                     /locus_tag="PLL59_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY24353.1"
                     /translation="MQKSNEEIELSVVVLCYHAENIIKRFVAQLINEISELQITYELI
                     LVANYDKNSIDNTPALATEIAKKYPNIVVLAHEKEGGMGWDMRSGLSAAVGKYIAVID
                     GDAQMPASDIPIVYGVIKFSNYDLVKTYRAKRYDGIIRSTMSSVYNILFRILYSPTFP
                     VRDINSKPKIFKKTAFQKMELQSNDWFTDAEIMI"
BASE COUNT         2303 a         1094 c         1222 g         2488 t
ORIGIN      
        1 aacaacttta gcagatagtt tgattgcgac tggatttcct tactacgatt ttgaacactt
       61 agataattat ttgtctgtat ttaaatactt aatgacttct acacgcggaa tacgccgctt
      121 aggttcggct gcaattgatt tgtgttttac tgcttgtggc cgatttgatg cattttttga
      181 atattcattg tcgccttggg acgtggctgc tggagcaatt attttacaag aagcaggagg
      241 aaaagtatct gattttgatg gcggaaataa ttggctgcat ggccgacaaa tgcttgcatc
      301 aaacgcattg ctttctgctg atctgttgca agtaattaaa gcacgatata aatagattta
      361 tgctaataat ttttgatttt taaaataaaa aaggttgcct ttgcaggcaa cctaaaaaac
      421 tgtgaaaact atgaaaaaga tttattgtgc gaaacttgct cttggaccgc cactggcaaa
      481 tgttgctaat attctagctt tttgtccact agagaacata tacatacaag catcatcagt
      541 ataatccatg aagttcatgg tcatctcgat tggtgtgcca ctgcatgtag atttatgacc
      601 agcagctggg caaccatagt ttgctgtgtt gtgtgtcggc gtgtcagaaa ctaagtcaga
      661 gccacatgtt gcatcgcccc aaatatgacg caagttcaac cagtggccaa cttcatgtgt
      721 tgctgttctg cctttgttga atggcgcagc agcagtacca gttctaccaa aacctttatt
      781 taagataacc acaccatctg tagaagagga gccacctgga aattgtgcat agcctaaaat
      841 accaccactt agcgtgcaaa cccaaaggtt taattttgta gttggtgaag ttggattaat
      901 acctccttgt gaagatttct tcatggcatc attagtgctc cagctttttt tagtagtttt
      961 ctttcttact acttggtcta atacaaagcg aatgcctacg tttgctttta cagcagaaaa
     1021 tgtagaaggc actaaactaa aatcagcatt tgtagcgtta aaatcttcat ttaatacatc
     1081 aatttgagat tgaatttgcg cagttgatac attttcagat gtagttctgt ataagacatt
     1141 cacgactaca ggaatttcaa ttacgccatt tactaaacgc gcgcttttgt tttctttgat
     1201 gttttgttga acaatagatt cataagcatt catacgttgt tctaaagttg gatcggcctt
     1261 tagttgttca gccaatactt ccattgaatt acagttacga tgagtcgcca ttgcgttatc
     1321 gtcagattcc attaaatttc ctttttggca cgcagagaac aaaagagcca ctgccgtgct
     1381 aatcaacatt agttttttca ttttttgaat tgttagtttt tgtttacgat actcgaattt
     1441 agaaaaggat ttctataagt ccaattattt aacaaaacaa tttgttaaca ttgaaaaatt
     1501 taatattgaa cagtaagatt acttttgaaa cattgggctg accaccagtt ctttactgcc
     1561 agcttcatat ttataaaaac cttcaccact ttttgcgcct aaataacctg cagttaccat
     1621 attgacgaga agtggacagg gtgcatattt tggattgccg aatccttcgt gcagcacacg
     1681 cataatggct aaacatacgt ctaagccaat aaaatcggca agttgcaatg gtcccattgg
     1741 atgtgccatg cctaatttca ttactgtgtc aatttctgaa atgcaagcaa cgccttcata
     1801 caacgagtag attgcttcat taatcattgg cattaatatt ctgttagcaa caaatccagg
     1861 ataatcgttg acttcaacag ggattttttg aagttgtttt gatacgtcaa aaacatgctg
     1921 attggtttcg ttagaagtag catagccacg aattacttcc accaatttca taataggcac
     1981 aggattcata aaatgcatgc ctatgacttt gtcagggcgt ttggtgcaag cagcgattct
     2041 agtaatagaa atagacgatg tgttgcttgc taaaatgcaa tgtggagcac atatttcatc
     2101 gagttcgcga aatatttttt ctttgatgct aaaattttca gatgccgctt caatgactaa
     2161 gtcactagca tgagcgcagt cttttataga agtatatgtt gtaatattgg caagtgtttg
     2221 tatttttaat tcttcagaca aaacaccttt ctgcacttgt ctgtctaagt ttttttgtat
     2281 tgtttgaata gccttatcta atgccaattg cgaaatatcg attaatgcta ctttaaaacc
     2341 aaattgtgca caaacgtgag ctatgccatt gcccatcgta cctgatccga taacgcttat
     2401 tgtgttgatt gccattttat ttagattttc tagctttaat actccattca aaatcaaaat
     2461 ctactaataa atcacctgct ttgtcgtaac cttttacatt gctttgaatt gtttgaggtt
     2521 ccatagtagt cattgccgca tgcactttgt ctgctataag ctggccatcg ttgcaaataa
     2581 aagtcgtgac accaatagca cgttttacaa acttgccact acatgccgtt acaaacatgg
     2641 aaacggaagg attggttttg tgtgtgtgca tttgcaccat tgcgccactt gccatttctg
     2701 ctgccatgcc tagtactgcc caataggtag tattaaaagg atttttgttg acataattat
     2761 atggaacagt tactttgcaa tgttgatcat ctaatgctgt tacttttaat ccagcaatgg
     2821 cgcccattgg cattaccttt agtaaaccag ctctaaacac tggcgataat accatgtttt
     2881 tatattcgtc tttttttgca ttcatatttg aaacctaatt gaacacaaga tataaaatgt
     2941 gtgtagatat tcgatgaata cttcaaaata attaacgatt gaatatgtat atttgtttaa
     3001 aattgtattg catgcaaaaa ccattacacg cattgtccaa taaatggact tttttattgc
     3061 ttttcattct ttcatttgta ctttatggaa ataccatttt taatgattat ggttatgatg
     3121 atgtttttgt tgtaaaggga aataaatatg tgcaagaagg cattgcagga ataccagcaa
     3181 tttttgagaa tccgtatgca gtagttgatt ctgttcagtt agataaacga cctattacat
     3241 tgattacttt tgctgttgaa catcagcttt ttaatgacaa tccacatatc agtcatttta
     3301 taaatattat tttgtatgcg ctaacattgt tggtgttgta tgttttatta caacaagctt
     3361 ttcaattaga aaaagtcaac aaatggctgc cattattagt tgttgtttta tttgctattc
     3421 atccattaca tactgaagta gtaagttcat taaaaaacag agatgaatta ttggtcatgc
     3481 tgtttggtct gctctatttg ttgttgagtt ataaatatgc acacgcttcc agtaggagag
     3541 tgttgtattt atcgctcgca attttttcat tagcgttaat gctcttgtca aaaattacag
     3601 gctttgtatt tgtatttatt ggtattgcca tgtttgtatt ttataaatgg cattctatta
     3661 ataaaagaaa ctggtttttt gtgttgtgga gtgtgctgtt attattcaga ggcttatatg
     3721 tcacattgga aggagtaaac agagaatttc tagtttttga aaatccatta atcaaagaga
     3781 caaatttgtt agtaaaaatt ggcactgcat ttttgatact actgcatcat attaagatgt
     3841 tgatttttcc aaatccaatg ttgttttatt atggtggaaa tacgtttcca ttggtgacac
     3901 tcggaaatcc tttagcattg ttttctatgt tattacatgt aacatttttt gtatatggct
     3961 gtgttaaatt tttcaagaga gaaatgatag gattgtttat tatttgctat tttatttcta
     4021 tcatgttata cgccaatttt ccgataccat acacgagtat gtatgctgag cgtactcagt
     4081 ttataagcag tttgtggttt atattaatat tggtttcact gtttaatttg ttgagttcgc
     4141 taaagccaat aaaaggtcat tttggtctta ataataagtt tgtactgatt gtattaagtg
     4201 ttgtatttat agtgtattca ttcttgacta tcaacaggaa tttttattgg aaaaattcat
     4261 tgattttaat ggaaaaggat atgccatatt tagaaaattc tatcattgca aattacattt
     4321 atgcaaataa tttaaagtat gaaagtaagc aagctatcga caccagtact tcaaaattat
     4381 tggcacaaaa agcagtgaca cactatcgac aagtgattaa attggttccc ggatttcctg
     4441 aatttcatta taaattagct agcacctaca gatataattt aaaaaattta gacagtgcag
     4501 aaatttatta tggttatgcc ttgagcttag attctattta tgctgatgct aactatgaga
     4561 tggctaaatt attttttgat aaagctgatt ttcaaagaag caattattat tttgaaaaaa
     4621 catatccatt gaagccaaca gattcgatga ctttatttta ttatggtcaa acagcattga
     4681 aaatgggcaa tctctcaact gcgtacaaaa ttgataaaga atttttgaac ctatatccaa
     4741 atcactatta tccatatgtc aatttaggcg tttattattc aactgcattg aaagatgata
     4801 gtgctgtaat ttattttgat aaagcgattg aaaaaggcta tcgcgaaccg agtttgctgg
     4861 cgaagttggc atattactac gaaacaaaag gaaatcaaga aaaagcaaag tattatagaa
     4921 gcttaagccg ctaaggtttc tcaaatttgt aaagaataaa gttgttgatt tttttttctt
     4981 ctttataatt cggataaaat gaagatacaa tttgctgctc actaactttg ttagttgtca
     5041 atatatattg aacacgtcca tcagcaaaat aggtatcata tttttgtgta ggtgcattat
     5101 ataattctga attacttttt aattttaata gcgcatatat taatctgtcc aaatgtattt
     5161 cgtggccata aataacacgt ttatctgcaa taaataaact atgttgaagc gttacatcat
     5221 agattgtcga atttgttttt aaatacgttt gcaatgcatc tctaagttga taattagatt
     5281 ggaatttttg tttttccttc ttttcttttt ttatattaat tgaataatta ttaagtacag
     5341 catttgatgc taataaaaat aaaatataaa aatcagcagc gatgattgtt tttttaagtg
     5401 gataattttt tagaaaataa attaatgcca atatgctaat gaggattagt tcatacgtat
     5461 agtttagata acttcctggt ctaaataaaa atatactact taatacagca gcaaaaatgc
     5521 cgctaattcc tagtaataaa ccaagctttt tttgtgttgt tttatgagaa ttgaataata
     5581 aaaaaataaa tagcatataa aatggtgaca atcgcactgt gttaaatagg acaatcataa
     5641 aattgtaaga ctctcgataa ttctttattt tttgcagatt atataagaat aaactttcta
     5701 tgcaataatt tccaaaaaat atgtgaaagg aaataaataa aaaaacagct agaataacaa
     5761 atgatattcc atataaaatg atagatttat ttcgttgaat taaaaaggta acaccaatta
     5821 ttaaagcaat ataaatgatg acatcttgct tagtagctac tgcgaataag gcagcaagca
     5881 aagaaacgta ttttgatttg acactgccat tttcataaaa ataatctata aaatgtatta
     5941 aaaaaattag gaacagtagc actttaaggc tgtcaggtct tgtggcgtat gcatgtccag
     6001 ttaatagtaa taaataaata aggcttatta acaaagcaat tgcttttgtt cctataattt
     6061 tcaatgaatg cttgtaaatt aataagactc caaataaagt gaatgcgaaa ctgtagcatc
     6121 gtccaacgat aaataattgt ctgatgtctg taatgtaatc gaaagaaaat attcttgccg
     6181 tatattcaac aatctttaaa tacaaaggtg tgtatatggt gattgagaaa ggaaaagttt
     6241 caggatttaa atataatctt tcacctttta atgtttgttg aatgtaatgt ataaatgtaa
     6301 attcagcgcc atctaaatca atagcgtatg aaaaaattaa taagaagcga acagctacaa
     6361 aataggcgat gagtaatagt aatatacttg tagcaacatt gtatttggtg agaaatgatt
     6421 tgatgtctga tggtttatgc gtgaatactc aactaaaata cagctatttt tatgtaatta
     6481 aataattttt tctgatttta aattgtattt ttgaaaaaac cagtatgcaa aaatccaatg
     6541 aagaaataga gctctctgtt gtcgtattgt gctatcacgc tgagaatatc atcaaacgtt
     6601 ttgtggcgca attaatcaat gaaatttctg aattacaaat tacttatgaa ttgatattgg
     6661 ttgcaaatta cgataaaaat tcaattgata acacaccagc attagcaaca gaaattgcta
     6721 agaaatatcc gaatattgta gtcttagctc atgagaaaga aggcggaatg ggttgggata
     6781 tgcgctctgg tttgtcggct gctgtcggaa aatatattgc agtaatcgat ggtgatgcac
     6841 aaatgccagc aagtgatatt ccaattgtat atggcgtaat aaaattcagt aattatgacc
     6901 ttgtaaaaac atatagagca aaacgatacg acggcattat tcgctcaacg atgtcgagtg
     6961 tgtataacat actttttagg attttgtact caccaacttt tccagttcgc gatatcaatt
     7021 caaaaccaaa aatttttaaa aaaacagcat ttcaaaaaat ggaattacaa tccaacgatt
     7081 ggttcacaga tgctgaaatc atgatac
//
LOCUS       DAOQQV010000127         7094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4344_length_7094_cov_7.121324, whole genome shotgun sequence.
ACCESSION   DAOQQV010000127 DAOQQV010000000
VERSION     DAOQQV010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4344_length_7094_cov_7.121324"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="PLL59_10880"
     CDS             complement(<1..1303)
                     /locus_tag="PLL59_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HMY24354.1"
                     /translation="MTQAQTDSLIEYGSRRVIITITVVLCTLLELIDTTVVNVATTTL
                     MGNLGATISEVSWVVAAYAIANVIVVPMSGWLSSTFGRKYYFAGSVAIFTFSSLMCGL
                     SNSIWTLVFWRFVQGIGGGALFATSQTILKEIYPPEKIGMAMAMFGMGVIVGPTIGPY
                     LGGYLVYNYSWPIIFYINIPIGIIAVFLTLTYIRDNPFEKKSDSSVDWLGIILLIVGI
                     GSLQLVLEQGERNDWFESSYIVMFTVAAVVGIIGFVIRELTAENPIVDLRVLKRGNVA
                     IGTIMNFVLGFILYGSVFIYPIYMQAFLGFTAEKSGEMFIPGALLSGMLMPVIGMLLT
                     KGMPPKFMITFGFTITGAFVYWSSFIFTTNTGADAMFWPLLVRGLGMAFLFVPLSGLT
                     LGGLNGKDVGQASGISNMVRQIGGSISVAVIGVLNETLSAEH"
     gene            complement(1342..2481)
                     /locus_tag="PLL59_10885"
     CDS             complement(1342..2481)
                     /locus_tag="PLL59_10885"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HMY24355.1"
                     /translation="MENTNNTTEKKSPKKIIAPIVGSLLLLAALLFAYKKITYAMHNE
                     DTENSQIESNIVPIAPRVQGYVDVIYVKENQHVKKGDTILKLDDRDLKIRLQQAIISA
                     KSAGANVNVVKSNAMSADASASAVSSSIVTAQAGIETAKANVDAAKVRVWNATENYKR
                     YEKLYNQTSATQAQYDAALAEKEAAEKQVAVLEKQVQVAQAQLKAAQQQASASRTQAS
                     GVGTQVNLAEVGIQQQQANVDFAQLQLSYAYLIAPCDGYIAKKNVQVGQLVNPGQILM
                     SVVDDSQLWITANFKETQIENMKVGQDVEVKVDAYPDHTFKGKIESVQAATGSKFSLL
                     PADNATGNFVKVVQRIPVRIALLDDKSDKYDLRAGMNVNVAVKVK"
     gene            complement(2510..3814)
                     /locus_tag="PLL59_10890"
     CDS             complement(2510..3814)
                     /locus_tag="PLL59_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMY24356.1"
                     /translation="MKRICIVSIILILAVQIKAQTIKKLNLQEAFELAIQHSKQLQID
                     SLKIQALDFKKKQAQNAMLPVVAINAGYTRLSDNIPDIIIAGGAFKIEQIVNQFNNRV
                     TLQQPIFQGLRNWNTMKSLDQQKEAASFDKQKNLQDVKLSVVQAYFSLYKLQQAKILL
                     DSNIAQTQTRVNDIIKFKDAGIVLNNDVMRAELQKTNLLVSKADVESAIDVMNYNMCV
                     LLGIENTTKIEVENPDIVNEATASIQSMVCTSFHERAELKAQEFRSKAADYMVKASKS
                     AYMPTINAVGNGMYNNPNQRMFPPEAKFKATWDIGVSLSWNIMQLYTARAIVGDAKNQ
                     KAQLEQVTSQIKDGIAMDVNANYEALNVALLKIDLANKAITQARENKRILDNRFDAQV
                     ALLSDVLEADQLLLQAQTNLLNAQADVAIANYKLQRSLGAIK"
     gene            complement(3816..4445)
                     /locus_tag="PLL59_10895"
     CDS             complement(3816..4445)
                     /locus_tag="PLL59_10895"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMY24357.1"
                     /translation="MSSEEKIQNDKRAHILDVAEYLFAHQGFEAVSIREISKAADINI
                     AMVSYYFGSKEKLYEAVVTRKLISSELILHHIERYNCSKDKLFVIVDLLTDKFFEHRN
                     MQTIIFREMAMEQRTAMAELIANQLHQNFTIINDVIQKGIKQKEFKKVDVELTVMTII
                     GIVKIYTTSSPVVCKVLKTEDKELMFSKQYKLRLKKYLKELLTNHLGIK"
     gene            4615..4959
                     /gene="gldC"
                     /locus_tag="PLL59_10900"
     CDS             4615..4959
                     /gene="gldC"
                     /locus_tag="PLL59_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HMY24358.1"
                     /translation="MARETEIKFKIKLDDQNLPEQIKWSASDGPQGDGDECKSMMVAM
                     WDGNEKNTLRFDLWTKDMQIDEMHTHFFQMLLSLGDTYHRATGNPFVQEDIKNFCMQL
                     GDKTREWEESKN"
     gene            5034..6941
                     /locus_tag="PLL59_10905"
     CDS             5034..6941
                     /locus_tag="PLL59_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMY24359.1"
                     /translation="MLQATNLSIIFGSRILFDNVTFTIQPRDRIGLVGRNGAGKSTLL
                     KAINGELTPQEGGISKPNGYTIGFLKQEIRGQHEPTVWLEAQTAFKEINILQQKLDDI
                     QHQLETRTDYDSDDYMNLSQDFCDLNDRLVHLDIDDKDKKTELVLLGLGYKRDDFEKA
                     PSTFSGGWAMRLELAKLLLQNPDLLMLDEPTNHLDIESIIWLEEFFQKYEGAIVLVSH
                     DKTFLDNVCNKTIELELGKMYEYKANYSKYLILKEDRREKQLQTFKNQQDQIKQIERN
                     IERFKAKASKATMAQSLVKKLDKMEIVEVEQDDVRSMRFSFPMGQQSGKIVVKATNLA
                     KNYGEKQVFHDVNLEIERGDKVAFIGKNGMGKTTMAKIIAKEIDYTSGNYDLGHNVNI
                     AFFAQHHAEHLPKELTILQYMEDTAPNEVRAQVRNILGAFMFSGDDATKKISVLSGGE
                     RARVCLAHLLLNPSNTLILDEPTNHLDIRAKEVLKDAISKYPGTVIVVSHDRDFLAGM
                     TNKVFEFNDGVVKEFVGDVNDFFEERRLANFREVEMRDETREKLEKTVVTNPKSQVSN
                     QNNQDKQLRNKISKLEEEISIKEFDIKKLEEKIEALSENGKYDEQIVLQLGTEKKVLE
                     KLMQDWEKLQA"
BASE COUNT         2330 a         1277 c         1188 g         2299 t
ORIGIN      
        1 gatgttctgc actcaatgtt tcgtttagca caccaataac agcaacactt atggaaccac
       61 caatttgacg aaccatatta gaaattccag aagcttgacc gacgtcttta ccatttaagc
      121 cacctaaggt aaggccagac aaaggcacaa ataaaaatgc cattcctaaa ccacgcacta
      181 ataatggcca aaacatggca tctgcacctg tattcgttgt aaaaatgaag gaagaccaat
      241 acacaaatgc gcctgtaatg gtaaagccga aagtaatcat aaatttgggt ggcattcctt
      301 ttgttaagag cattcctatc actggcataa gcataccact taacagcgcg cctggtataa
      361 acatttcacc agatttttcg gccgtaaagc ctaaaaatgc ctgcatataa attggataaa
      421 taaatacact tccgtacaaa ataaatccta atacaaagtt cataattgta ccaatggcaa
      481 catttcctct ttttaaaaca cgtaaatcta caattggatt ttctgctgtc aactcacgaa
      541 tgacaaagcc aataattccg acaacagcag caacagtaaa catcacaata taactcgact
      601 caaaccagtc gttgcgctca ccttgttcta ataccaactg caatgaacca ataccaacta
      661 ttaataaaat aatacctaac caatcaacac tcgagtctga ttttttttcg aatggattat
      721 ctctgatgta agttaaagtt agaaatactg caatgatacc aattggaata ttgatataaa
      781 aaataattgg ccatgagtaa ttatatacca aatatccacc taaataagga ccaatcgttg
      841 gaccaacaat tacgcccata ccaaacattg ccattgccat gcctatcttt tcaggtggat
      901 atatttcttt taaaatagtt tgtgaagtgg caaataaagc gccaccaccg atgccttgca
      961 caaatcgcca aaacactaaa gtccaaatag aatttgacaa accacacatc agcgatgaaa
     1021 atgtaaaaat agcgacagag ccagcgaaat aatattttct accaaaagta gaagacagcc
     1081 aaccactcat tggcacgact attacattgg caattgcata ggcggcaacg acccagctta
     1141 cttcagaaat agtagcacct aaattgccca ttaatgttgt agttgccaca tttactactg
     1201 tggtgtcaat caattctaat aaagtacaca acacaactgt aatggtaata attactcgtc
     1261 gtgagccata ctctatcaaa ctgtctgttt gtgcttgtgt cattttacag ttattgcatt
     1321 taagcgttta taatttagca attacttcac ttttacagct acatttacat tcatgccagc
     1381 gcgcaaatca tacttatctg atttatcatc taacaatgca atgcgaaccg gaatacgttg
     1441 taccactttc acgaaattgc ctgttgcatt atcggcaggc aacaatgaaa atttagagcc
     1501 tgtcgcagct tgtacagatt cgatttttcc tttgaaggta tggtcaggat aggcatcgac
     1561 ttttacttct acatcttgac caactttcat attttcgatt tgtgtttctt taaaattagc
     1621 tgttatccaa agctgtgaat cgtcaactac actcattaat atttgtcctg gatttaccaa
     1681 ctgtcctact tgcacatttt tcttagcaat ataaccatcg caaggagcaa ttaaataagc
     1741 ataagacaat tgcaattgtg caaaatcaac atttgcttgt tgttgttgaa tgccaacttc
     1801 tgcaagattt acttgtgtgc caacaccact tgcttgtgtg cgtgatgctg atgcttgttg
     1861 ctgtgctgct tttagttgag cttgcgccac ttgtacttgt ttttctaata ctgcaacctg
     1921 tttttctgct gcctcttttt cggctaatgc ggcatcgtat tgtgcttgtg tcgccgaagt
     1981 ttgattatat aatttttcgt agcgtttata attttccgtt gcattccaga cacgcacttt
     2041 agcagcatcg acattggctt ttgcggtttc aatgccagcc tgtgctgtta caatagatga
     2101 agaaacagca cttgcagaag catcggcaga cattgcatta ctttttacta cattgacatt
     2161 tgcacctgca ctttttgcgc taataatagc ttgttgcaat ctgattttta gatctctatc
     2221 atcgagtttt aatattgtat ctcctttttt gacgtgttga ttttctttta cataaatcac
     2281 atcaacataa ccttgcacgc gtggcgcaat tggtacaata ttactttcta tttgagagtt
     2341 ttcagtatct tcattatgca tagcatacgt tattttttta taagcaaata acaatgctgc
     2401 gagtaacaat aaactgccaa caattggagc aattatcttt ttaggtgatt ttttttcagt
     2461 tgtattattt gtattttcca taattgaatt attctattat ttcgtaaaat tatttaattg
     2521 caccaaggct tctttgtaat ttataattag caatcgctac atcagcttgt gcgtttaata
     2581 gattagtttg tgcttgtaat agtaattggt ctgcctctaa cacatcgctt agtaatgcga
     2641 cttgtgcatc aaagcgatta tctaaaatgc gtttattttc tcttgcttgt gtgattgctt
     2701 tatttgccaa atcaattttt agtaaagcaa cattcaaggc ttcataattt gcatttacat
     2761 ccatagcaat gccatctttg atttgtgaag taacttgttc caattgtgct ttctggtttt
     2821 ttgcatcgcc aacaatagca cgtgctgtat agagttgcat aatattccaa ctcaaactta
     2881 caccgatatc ccaagttgct ttaaactttg cttctggagg aaacatacgt tgattgggat
     2941 tgttatacat tccatttcca actgcattta tggtaggcat gtaagcactt tttgaagctt
     3001 taaccatata atcagcggct ttacttctaa actcttgcgc ttttaattca gcgcgttcat
     3061 gaaaagacgt gcacaccata gattgtatgc ttgctgtcgc ttcattaaca atatctggat
     3121 tttcaacttc aatttttgtc gtattctcta tacctaataa gacacacata ttgtaattca
     3181 tcacgtctat tgcactctct acatcggctt ttgatactaa taaatttgtt ttttgcaatt
     3241 cggcgcgcat cacatcatta ttcagcacga tacctgcatc tttaaatttt atgatatcat
     3301 ttacacgagt ttgtgtttgt gcaatattgg aatccaataa tattttagct tgttgtagtt
     3361 tatataaact aaaatatgct tgtacaacac tcagtttcac atcttgcaga tttttttgtt
     3421 tgtcaaagct tgcagcttcc ttttgttggt ctaaagattt catcgtgttc caattccgta
     3481 aaccttgaaa aataggctgt tgcaaagtca ctctattatt gaattgattg actatttgtt
     3541 ctattttaaa tgcaccgcca gcgataatga tatcaggaat gttatcactc aatctcgtat
     3601 agcctgcatt aatagccact acaggcaaca tggcattttg tgcttgtttc tttttgaaat
     3661 ctaatgcttg aattttaaga ctatctattt gcaattgttt gctatgttgt attgccaact
     3721 caaatgcttc ctgcaaattc aatttcttaa tcgtttgtgc tttgatttgc actgctaata
     3781 ttaggattat cgatactata caaattcttt tcatcttact ttattcctaa atgattcgtt
     3841 aataattctt ttaaatattt tttcaatcgt agcttatatt gtttgctaaa catcagttct
     3901 ttatcttctg tttttaaaac cttacaaacg acaggacttg atgttgtata aattttcacg
     3961 atgcctataa ttgtcattac tgttaattca acatcgactt ttttaaattc tttttgttta
     4021 attcctttct gaataacatc gttaatgatg gtgaaatttt gatgtagctg attggcaatt
     4081 aattcagcca ttgcagttcg ttgttccatt gccatttctc ggaaaatgat agtctgcata
     4141 tttctgtgtt caaaaaactt atccgtcaat aaatctacta taacaaaaag tttatccttt
     4201 gaacagttgt atcgttcaat atgatgtaga atcaattctg aagaaatcaa cttgcgagta
     4261 acaactgctt catatagctt ttcttttgag ccaaaataat atgataccat cgcaatattt
     4321 atatcagctg ctttcgatat ttcacgaata ctgacggcct caaatccttg atgggcaaat
     4381 aaatattctg ctacatcaag aatatgcgca cgtttatcat tttgtatttt ttcttccgac
     4441 gacatcagtt aaacacttgt ttaattttgc attgcaaatt taattaaacg tttgtttaat
     4501 ttgcaagtta aatttatgta aactaaataa tgtgtgagtc cataaaattg agttacaata
     4561 aaataaatta gcataagact tccatttcgt atatttgcac ttcaattttt accaatggca
     4621 cgcgaaacag aaataaaatt taaaataaaa cttgacgacc aaaacttacc agaacagata
     4681 aaatggtcgg cgagtgatgg tccacaaggc gatggcgacg aatgcaaaag catgatggta
     4741 gcgatgtggg acggcaacga aaaaaatacc ttgcgtttcg acctatggac aaaggacatg
     4801 caaatagatg aaatgcatac tcactttttt caaatgctat taagccttgg cgatacatat
     4861 cacagagcta ctggaaatcc attcgtacaa gaagatatca aaaacttttg tatgcaatta
     4921 ggtgataaaa cacgcgagtg ggaagaatct aaaaattgaa ataattaaaa aatgtaataa
     4981 tgtatcaatg aggcattgtt acattattga attaataaat tactacatta aaaatgttac
     5041 aagcaactaa cctttctatt atattcggca gtcgtatttt attcgacaat gttaccttta
     5101 caattcaacc gcgtgataga attggtttag tcggcagaaa tggtgctgga aaatctactt
     5161 tattaaaagc aatcaacggc gaattaactc cacaagaagg cggcatttca aagcctaatg
     5221 gttatacgat tgggttttta aaacaagaaa ttcgaggaca gcacgaacct acggtttggc
     5281 tcgaagcaca aacggcattc aaagaaataa atattttaca acaaaaatta gacgacattc
     5341 agcatcagtt ggaaactcgt accgactacg atagtgatga ttacatgaat ctatcgcaag
     5401 atttttgtga tttgaatgat cgcttagtgc atttagatat cgatgataaa gacaaaaaaa
     5461 cagaattagt tttattagga cttggttata aaagagacga ttttgagaaa gcgccttcta
     5521 ccttttctgg tggctgggcc atgcgattag aattggcgaa attattatta caaaatcctg
     5581 atttgttgat gctcgatgag ccaaccaacc atttagatat cgaatctatc atctggctcg
     5641 aagaattttt tcaaaaatat gaaggtgcca ttgtattggt ttcgcatgat aaaacttttt
     5701 tggataatgt atgtaataaa actatagaat tagaattagg aaaaatgtat gagtataaag
     5761 ccaattactc aaaatacctt attttaaagg aagatcgacg cgagaagcaa ctacaaactt
     5821 ttaaaaacca acaagaccaa attaaacaaa ttgagcgaaa tatcgaacgc tttaaagcaa
     5881 aagcgagtaa ggcgactatg gcgcaaagtt tagttaaaaa attagataaa atggaaatcg
     5941 ttgaagtgga acaagatgat gttcgctcga tgcgatttag ttttcctatg ggtcagcaaa
     6001 gcggcaaaat agtagtaaaa gctactaact tggcgaaaaa ctatggcgag aaacaagttt
     6061 tccatgatgt caatttagaa attgaacgtg gcgataaagt agcatttatt ggaaaaaatg
     6121 gaatgggaaa aacgacaatg gcaaaaatca ttgctaaaga aatagattat acttctggta
     6181 attacgattt aggacacaat gtcaatattg cattttttgc gcaacatcat gcggaacatt
     6241 tgccaaagga attgaccatt ttgcaataca tggaagacac tgcaccgaat gaagttcgtg
     6301 cgcaagtacg caatattctc ggtgcgttta tgttcagtgg cgacgatgcg acgaagaaaa
     6361 tttctgtgct gagtggcggc gagcgtgcac gtgtttgttt agctcatttg ctattaaatc
     6421 cgagcaatac attaatcctc gatgagccga caaatcactt agacatacga gctaaagaag
     6481 tattgaaaga tgcgatttcc aaatatcctg gcacggtgat tgtcgtttcg cacgaccgcg
     6541 attttttagc aggcatgacc aacaaagtat ttgaatttaa tgatggcgtg gtaaaagagt
     6601 ttgttggcga tgtgaatgat ttctttgaag aaagaagatt ggctaatttt agagaagtag
     6661 aaatgagaga tgagacaaga gagaaattgg aaaaaacagt tgtcacaaat cctaaatctc
     6721 aagtctcaaa tcaaaataat caagataagc aactcaggaa taagatttca aaactagaag
     6781 aagaaatttc tatcaaagaa tttgatatca aaaaattaga agaaaaaata gaagcacttt
     6841 ctgaaaatgg taaatatgac gaacaaatcg tattgcaatt aggaacagaa aaaaaagtat
     6901 tagaaaaatt aatgcaagat tgggaaaaat tgcaagcata gaaaatcata tacttagcaa
     6961 atcaacttgg caaacacgtt gtgcggcttg aatttagttt gcatttttta tattcaactt
     7021 attatcaagt tcaaacttct gtcacttttt ttcttagtca aaaaaagtaa ccaaaaaaga
     7081 caaggcaaaa atat
//
LOCUS       DAOQQV010000128         7012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4437_length_7012_cov_7.759954, whole genome shotgun sequence.
ACCESSION   DAOQQV010000128 DAOQQV010000000
VERSION     DAOQQV010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4437_length_7012_cov_7.759954"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLL59_10910"
     CDS             complement(<1..1028)
                     /locus_tag="PLL59_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HMY24360.1"
                     /translation="MNRSEFLKLCAILGIGSTIPKSILANDLFNDLRSSDFGNDFVWG
                     TATASYQIEGAWNVDGKGESIWDRFAHQPRNIKTKENGDVACDFYNRYNEDIELMRDM
                     HIKHSRFSISWPRLLPEGTGKINQKGIDFYDRVIDKYLKENITPWATLYHWDLPQKLQ
                     DKGGWENRDILGWFEEYVSLCAHKFGDRVKNWMVFNEPGTFVPLGYLIKIHAPGKFGF
                     NHFLPALHHVSLAHGVGGRVLKNVVKDGNIGTTFSCSHVDPYKNNERFIQSAKRMDAF
                     TNRLYIEPILGLGYPTAEIPKLKKMEKYFKPDDEKNLPFDFDFIGLQNYSRIVSKPLQ
                     IVPGLHAI"
     gene            complement(1036..3459)
                     /locus_tag="PLL59_10915"
     CDS             complement(1036..3459)
                     /locus_tag="PLL59_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMY24361.1"
                     /translation="MNKTFFLLLLTFCSITFLGAQTATVKGLVKQENNNEPLIGVNVS
                     VQGTNDGTTTDLDGLYELKLAPGSYTIVFTYVGYDDRTETVVLAANETKTLDIAFTEN
                     TNLLSTVVVSGSRYEKKLSEEIVSMDVIKPQFIEKQNITDLGSAVRRNPGVTIIDGQF
                     NIRGGSGWSFGAGSRVSILLDGYPVLQPASGSSSGLGLPMESVGQIEIIKGAASALYG
                     SSALNGIVNLRTAHATTDPETTIAFYGTVNDNPTKTEKYFDAAGNLQTEKIDKRWWVL
                     DSVYLPGVGFTGKDTTILNENKTRPHSFGFTFSNRQKIGRVDLVMGGQFSRHVGMYWG
                     TQGASGRFNVGARYNINTKWRVGINVNLSFGKGASTFLWGNYRGVNKYVPSLDMMPTQ
                     SKYYNGSVDPYMNYSDTKGNSHKILTRYLGTLNNNSDNRSNGSTILYGEYQYQRRIDK
                     INMNISTGIVAQYNFTSKSPLYGNEILKADNEAFYVQLDNKFWKRLSTSVGFRLETNK
                     MTDTKREVKPVFRVGVNVEAAKYTFIRASFGQGYRFPSIAERFILTDFSGFAIVPNPI
                     LKSETGFSAELGIKQGIPFGKNFNAFIDVAGFYTEYTNMMEFTPVGVGSPIPTPPGSV
                     LSFASQNVGNTRIYGTEVSLTGAGKLYKFPTTLVIGYTYIKPEFKKFDPIVAFGVADY
                     NILKYRFRHTFTAMWDIDFKGFGFGTNVQYFSALENYDTIFDIIGVGIEQFRNTTLKK
                     NAANLPARKRFKGSATWDLRASYGFGKNSKYTLSFLVNNVLNKEFSLRPGILENNRTY
                     TVRIEMKFK"
     gene            complement(3480..5147)
                     /locus_tag="PLL59_10920"
     CDS             complement(3480..5147)
                     /locus_tag="PLL59_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HMY24362.1"
                     /translation="MHQLSLTDYFVFIAYFLIISFYGWFIYTKKKSAQNNSTDYFLAE
                     GTLTWWAIGASLIASNISAEQFIGMSGSGFKMGLAIASYEWIAAITLIIVAVFFMPVY
                     LNNGIYTMPQFLEKRYGKSIALIMAIFWMFLYIVINLTTILYLGALAINSITGFSIYA
                     CMILLALFALFITLGGMKVIGYTDVIQVVFLIFGGLVTTYIALNLVAEKTGTTGVVNG
                     FSNLIQHAPDHFHMVFKKENPNFMDLPGLTVLLGGMWIANFSYWGCNQYITQRALGAD
                     LQTARRGILFAAFLKLLMPIIVVLPGIAAYVLYQNGSFANEMTVNGQLVVDKAYPTLL
                     NLLPIGLKGLAFAALTAAVVASLAGKTNSISTIFSLDIYKKYINKDASEKQLISVGKI
                     VIIFSLIIAVIIAPLLGIDKQGGFQVIQEYTGFISPGILALFLLGMFWKKTTNKAAIF
                     GALASLAFSVFLKFIPQYIDLSFLSTLGFAKANADGIYEIPFLDRMGFVFLFCIIGMI
                     LISLLSSDEKQQAQLNINKQQFKVDKLFFIGSAIVIILLVLLYTIFW"
BASE COUNT         2327 a         1192 c         1109 g         2384 t
ORIGIN      
        1 tttatagcat gtaatcctgg aacaatctgc aaaggtttgc ttacaatacg agaataattt
       61 tgcaatccaa tgaaatcaaa atcaaaaggc agattttttt catcatctgg tttgaaatat
      121 ttttccattt ttttaagttt aggaatctcc gctgtaggat aacctaaacc caagataggt
      181 tcgatgtaca agcgattggt aaaagcgtcc atacgtttag cagactgaat aaatcgctca
      241 ttgtttttat atggatcaac atggctacaa gagaatgtag tgccgatatt gccgtctttt
      301 actacatttt ttagcacacg accaccaacg ccatgtgcta atgaaacgtg atgtaatgca
      361 ggtaaaaaat gattaaagcc gaatttgcca ggcgcatgta tttttataag atagccaagc
      421 ggtacaaatg ttccaggctc attaaaaacc atccagtttt ttactctatc gccaaattta
      481 tgagcacaga gagatacata ttcttcaaac cagcctaata tatcacgatt ttcccaacca
      541 cctttatctt gtagtttttg tggcaaatcc caatggtaaa gtgttgccca aggtgtaatg
      601 ttttctttga gatatttgtc aattacgcga tcgtaaaaat cgattccttt ttgattgatt
      661 tttccagtgc cttctggcaa aagtctaggc cagctgatag aaaaacgaga atgcttaatg
      721 tgcatgtcgc gcattaattc aatgtcctca ttatatctat tgtaaaaatc gcaagcgaca
      781 tcgccatttt ctttagtttt gatgttgcgt ggttgatgtg caaatctatc ccaaatcgac
      841 tcaccttttc catctacatt ccaagcgcct tcgatttgat aggaagcagt agctgttccc
      901 caaacaaaat catttccaaa gtctgaactt cttaagtcgt taaaaaggtc attcgctaat
      961 atgcttttag gtatcgtaga gccgatacct aaaatagcac atagttttaa aaattccgat
     1021 cgattcattt atctattatt taaatttcat ttcgattcta acagtatatg ttctgttgtt
     1081 ttctaagata cctggacgaa gcgagaattc tttgtttaaa acattattaa ctaagaaaga
     1141 taaggtgtat ttgctgtttt taccaaatcc atagctcgct cttaaatccc aagttgctga
     1201 gcctttaaat ctcttacgtg caggtaaatt agcagcattt ttctttaatg ttgtatttct
     1261 gaattgttcg ataccaacac caatgatgtc gaatatagta tcgtagtttt ctaatgcact
     1321 gaagtattgt acgttagttc caaatccaaa tcctttgaag tcgatgtccc acatagctgt
     1381 gaatgtgtgt ctgaatctgt attttaaaat attatagtca gcaacaccaa atgctacgat
     1441 aggatcgaat tttttgaatt caggtttgat gtatgtgtaa ccaattacta atgtagttgg
     1501 gaatttatat aattttcctg ctcctgtaag gctgacttca gtaccataga ttcttgtatt
     1561 tccaacgttt tgtgaagcaa atgacaatac agagcctggt ggtgttggta ttgggctgcc
     1621 cacaccaact ggtgtgaatt ccatcatatt ggtatattct gtatagaaac cagcaacatc
     1681 gataaatgca ttaaagtttt tgccaaaagg aataccttgt ttgataccta attccgcact
     1741 aaagccagtc tcagatttta agattggatt cggaacgata gcaaaaccgc tgaagtctgt
     1801 taagatgaaa cgctctgcaa tagatgggaa acggtaacct tgtccgaatg aagctctaat
     1861 aaatgtgtat tttgccgctt caacgttaac acctacacgg aatacaggtt ttacttcacg
     1921 tttagtgtct gtcattttat tagtttctaa acggaagcca acagatgtgc ttaggcgttt
     1981 ccagaattta ttatctaatt gtacataaaa tgcttcattg tctgctttca aaatctcatt
     2041 gccatacaaa ggcgatttag atgtgaagtt gtattgtgcc acgataccag tagagatatt
     2101 catgtttatt ttgtcaattc tacgttgata ttgatattca ccatataata ttgtactacc
     2161 attgctacgg ttatcagagt tattatttaa tgtacctaaa taacgggtta atattttgtg
     2221 gctattgcct tttgtatcac tataattcat ataaggatca actgagccat tatagtactt
     2281 actttgtgtt ggcatcatat caagacttgg cacatatttg tttacgcctc tgtaatttcc
     2341 ccaaaggaag gtgcttgcac ctttaccgaa cgataagttt acgttaatcc ctacacgcca
     2401 ttttgtattg atgttgtagc gtgcgcctac attgaaacga ccacttgcgc cttgtgtgcc
     2461 ccaatacata cctacatgac gagaaaattg accacccata actaaatcta ctctaccaat
     2521 tttttgtctg tttgaaaaag taaaaccaaa gctatgtggt cttgttttat tttcatttaa
     2581 tattgtagtg tctttgcctg taaaacctac acctggaaga tatacactat ctaaaaccca
     2641 ccatctttta tcaatctttt cagtttgtag gttgccagca gcatcgaaat atttctcagt
     2701 ttttgttggg ttgtcattta cagtaccata aaatgcgatt gttgtttcag gatctgttgt
     2761 tgcgtgtgca gttcttaagt tgacaatacc atttaatgct gatgagccat ataatgcaga
     2821 tgcagcacct ttgatgattt cgatttgtcc aacgctttcc attggcaagc ctaagccaga
     2881 gctactgcca cttgccggtt gaagcactgg atagccatca agcaaaattg aaactcttga
     2941 tccagcgcca aaactccagc ctgagccacc gcgaatatta aactgaccat caattatagt
     3001 aacaccaggg tttctcctaa cagcagaacc taaatctgta atgttttgtt tttctataaa
     3061 ttgcggtttt attacatcca tagagacgat ttcttcactt aattttttct catatctact
     3121 gccagaaaca acgacagtgc taagcaaatt agtgttttcc gtaaacgcaa tgtctaatgt
     3181 tttagtttcg tttgctgcga gtactactgt ttctgttctg tcgtcatagc caacgtatgt
     3241 aaaaacaatt gtgtagcttc ctggcgctaa ttttaattca tagagtccgt ctaaatcggt
     3301 tgtcgtacca tcatttgtgc cttgtacaga cacgttaacg ccgattaatg gctcattgtt
     3361 attttcttgt tttacaagac ctttaacagt agctgtttgt gctcctaaga atgtaatgga
     3421 gcagaatgtg agtaatagta ggaaaaatgt tttattcata aaaaagtgtt tgaatttgct
     3481 taccaaaaaa tagtataaag tagaacgaga agtataatta ctattgcact accaataaaa
     3541 aataatttat ctactttaaa ttgttgtttg ttgatgttta attgcgcttg ttgtttttca
     3601 tcacttgata ataaactaat aagaatcata cctattatac agaacaagaa gacaaacccc
     3661 attctgtcta aaaatggaat ttcataaatg ccatctgcat ttgctttagc aaatcctaaa
     3721 gtagacaaga aagataaatc tatatattga ggtataaatt ttagaaaaac agaaaaagct
     3781 aagcttgcca atgcgccgaa aatagctgct ttatttgttg tttttttcca aaacatacct
     3841 aataaaaata aagctaaaat acctggactg ataaatcctg tgtactcttg aattacttga
     3901 aaaccacctt gtttgtcaat acctaacaaa ggagcgataa tcactgcaat aatcaatgaa
     3961 aatataatga caattttgcc tacagaaatc aattgttttt cggaagcatc tttgttaatg
     4021 tattttttat agatgtctaa actaaatata gtagagatgg aatttgtttt gccagcaagt
     4081 gatgcgacaa cagccgctgt taatgctgca aatgctaatc ctttcaatcc aataggtaat
     4141 aaatttaata atgtaggata ggctttgtca accacaagct gaccattaac tgtcatttcg
     4201 tttgcgaagc ttccattttg atataataca taagcagcaa tacctggaag cacgacaata
     4261 atcggcatta ataattttaa aaatgcagcg aataaaattc ctcgtctcgc ggtttgtaag
     4321 tcagcaccta aagcacgttg tgtgatatat tgattgcaac cccaataact aaaattagca
     4381 atccacatac cacctaataa tacggttaag cctggcaaat ccatgaaatt gggattttct
     4441 tttttaaata ccatgtggaa atgatctgga gcatgttgta ttaaatttga aaaaccatta
     4501 acaacacctg ttgtgcctgt tttttcagct acaagattta atgcaatata ggtagtgacc
     4561 aagccaccaa aaattaaaaa tactacttgg ataacatcag tataaccgat tactttcatg
     4621 ccacctaatg tgataaataa tgcaaataag gctaataata tcatacacgc ataaattgaa
     4681 aaaccggtga tactattaat agcaagtgct ccgagatata agatagtcgt taaattaata
     4741 actatatata agaacatcca aaaaattgcc atgattaatg caatactttt gccatatctt
     4801 ttttctagaa actgtggcat cgtataaatg ccattattta aatatacagg cataaaaaaa
     4861 acagccacta ttatcaatgt aatagctgca atccactcat agctggcgat ggctaatccc
     4921 attttaaaac cacttccgct catgccaata aattgttcgg cagaaatgtt tgacgctatt
     4981 aatgatgcgc cgattgccca ccacgtaagc gttccttcag ctaagaaata gtcggtagaa
     5041 ttgttttgtg cgcttttctt ttttgtataa ataaaccagc cataaaacga aataattaaa
     5101 aaataggcta taaagacaaa atagtcagta agagacaatt ggtgcataat aaatgtattc
     5161 tttcttgttt acgttacaag gtacttattt tccgttaatg gcaaacaata gtaattacga
     5221 ttttttaaca ttattgactt ttttttgata aatgtggact ttactcgatt ttagacaaca
     5281 aacttgattt gtattagaca atataataga agtaggagtg gagaaatgaa tcatatctgt
     5341 tttttgtttc atctttttag tgcaaattgt tctatttagt aaatacgatt gcttagccat
     5401 catgcatgta tatatagaaa accattaata agtatagtct tatttgatct gtataaaaac
     5461 agaaatatta cttctgattt atgtaatcag cattacttta gtttaagaaa aatgactaag
     5521 ttttattgtg tttgtttttt ggaatttatt gaataaatag attcttgttc ttttattgaa
     5581 ctggcgacat atcaagtgag gtaattatta atctttagta atcgtgtaca gattttcaat
     5641 aagactgaat gtattcaagt ttgcaggata ggagagcgca ttcatgaact attactatac
     5701 tactctcaaa tctatataat ataagaatct acttttaagc aaatcgtata gaactgtaca
     5761 gagtttttct aatgattatt cgattttcag attcgatttt taattttttt tgagattcac
     5821 catagcataa aaattataat catgtatttg atgtatgttt tttcatatga aataattgaa
     5881 gttgtgccga aaaatcaagc ttagattatg cttttccatt ctatttatat atgaaagatg
     5941 tagcaaataa aataaaaaga cgctatttta atattcttag actatttttt tcgattgatt
     6001 ttcttcaaaa cagaaattgc ttttgatatt atttgttatt caattggaat aaaaatgaaa
     6061 aaaacaatct ctttttattg cacaagccaa atatttttca aaatctattc tttcattcaa
     6121 ttattatgta catcatatag aaacgtgtga aaacagcagt aaaataaggc tgtttgacga
     6181 tttgttcttc caatttcata caattaaatg aagagcaacg gtatatcatg tttgcatata
     6241 caatcgacat gttttactca atttatcata aaatgagttt attagtattt ctacatcgaa
     6301 ggcaattatt gaaatgtagt aaataaatca actatttggt ttatagtgtt ttaatacgtt
     6361 tttattaaaa ctgttaaaaa aaaaggaatt ccaaattact tatattaaca atgcttcaaa
     6421 gaaactaaac aattacttaa actacgtcaa actatcaata aaatcaagtg ttttagcaaa
     6481 tgtctcgata aaatgttttt ttattttcat acaaacactt gcgaattaaa aaacacttac
     6541 tacctttgca tccgcttttg aaaacaaaca caaaaacgaa ttcagaagca gtgttctttc
     6601 aaatactaca aaatgtcaat cacgaaaaat atttttaatt ttttttcaaa taaaatttgg
     6661 aaattaaaaa aagagtgtta cctttgcacc cgcttttgaa acgaactaat aaaaattcaa
     6721 ttcaaaacga gttctttaaa acggtaataa aagtgtggat aaaattacca aaaataattt
     6781 tccacttttt gttgacaatt gaaagaaagt ttctatcttt gcagtccctt ctgaaaaaaa
     6841 atcagaagat aagcaaaaaa gttctttgat ttattgggga gccttaatag gttcaaaaaa
     6901 atgaatgata cacactcaaa tcatattaat ttaaaacaaa ctcgtactaa aagtacatta
     6961 caacggagag tttgatcctg gctcaggatg aacgctagcg gcaggcttaa ta
//
LOCUS       DAOQQV010000129         6853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4622_length_6853_cov_7.862607, whole genome shotgun sequence.
ACCESSION   DAOQQV010000129 DAOQQV010000000
VERSION     DAOQQV010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4622_length_6853_cov_7.862607"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLL59_10925"
     CDS             complement(<1..473)
                     /locus_tag="PLL59_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24363.1"
                     /translation="MLKPLTLMARVLAIFSITIVQAQSTNNSSMLCYISADKTTHLVD
                     TKLIPAQPYTITCTYNEKDASIPKFIQFSCSGASVITVDVHVTNGTTTQKISKDIRIQ
                     ANRAVYEVPVLAYVNDNIMQSGNARINAIEFSNTLNQEAHLNEVYFTNTSSNYEV"
     gene            630..956
                     /locus_tag="PLL59_10930"
     CDS             630..956
                     /locus_tag="PLL59_10930"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HMY24364.1"
                     /translation="MNDFYEKVYRLVAKIPRGKVCTYGDIATFLGTKGSARTVGYAMN
                     HAQSSTFYVPAHRVVNRNGLLTGKHHFESPTMMQELLESEGIKVIQDQIQDFEKVRWI
                     PKLKIK"
     gene            complement(1036..2151)
                     /gene="dnaN"
                     /locus_tag="PLL59_10935"
     CDS             complement(1036..2151)
                     /gene="dnaN"
                     /locus_tag="PLL59_10935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HMY24365.1"
                     /translation="MRFVVSSAQLFKNLQKIGGVISTNTVLPILEDFLFDIDKNILNI
                     TATDLDTTMSVSMDVESKESFKVAIPARILLDSLKALAEQPITIAVDETTNGIEITTD
                     NGKYKLSGENSADFPKEPTAEGVDEVKLASSILNKGISKTLFAVSNDELRPAMTGVYF
                     QLDDNGITFVATDAHKLVKYNHSEIKGGNASFIVPKKALNLLKAVLQNNDTEVSVQFN
                     KSNAFFSFNNIQLICRLIDAKYPDYNAVIPKENPNLLSVQKEDFFSSLKRTSIYSNKT
                     THQVVLKMAGSELTVSAQDLDFSNEASEKLSCDYQGNAMEIGFNAKFLIEMLAALDSS
                     DINIELSTPNRAGIIRPTDKDENEDLLMLVMPVMLNN"
     gene            2274..3404
                     /locus_tag="PLL59_10940"
     CDS             2274..3404
                     /locus_tag="PLL59_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMY24366.1"
                     /translation="MKIAINTRFLLKNKIEGIGMFTQEIFKRVVQLMPEHEFYFLFDR
                     AYDESFVFAKNVIPITISPPARHPVLWYIWFEHAIPKVLKKHHIDLFISPDGFGSLST
                     DVPQIVTIHDLGFEHYPEHTPFLVRHFYQYFTPKYCQKASKLIAVSNATKQDIISKYG
                     VDENKISIVFNGFEDCRYIINNEQAQSLSIIDKRISDKTPYFIFIGAVHPRKNVLNLL
                     KAFELFKQTFTLNYQLLIVGRNAWMNAELETYYQQMKYKNDVIWIENIERADLLQLLQ
                     NAFALCYPSWFEGFGIPIIEAMSLGVPVITSNTSSMAEIAANAGIMVAPDNIQEIATA
                     MHLLTSDNALRQDLIDKGKIRAKEFDWNKSALQVVQLIKTYE"
     gene            complement(3414..4391)
                     /locus_tag="PLL59_10945"
     CDS             complement(3414..4391)
                     /locus_tag="PLL59_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24367.1"
                     /translation="MKKILTIVFLGLVVLVSAQNTNYTVNNLSPELTAITENVKKEAN
                     LNPEQFKKFKADYVLFLNENAKPNANTNTLFMLLGLKFRGYMNDGQFSKVTQMVKDGK
                     LNPPGNKTDLTKSTNKPTTPANTSASVPKVNGLLPASISNQSNVTALFEQLQSFMKIT
                     PEKAAQVIPILKSYDKQLTKIKTDNNGNAAKIKQQTDVLNGQTVPQLKQHMTDQQIAT
                     LVLALGMQENILSGKNLNAEQKIFLDKIRHQYQLNDVQTMSVVLVLVQGKIRGDAIAL
                     LHKSNPEQAGKEFLFLLQDLDKQLQASLTNEQYVKVKADIEKLLKGQKL"
     gene            complement(4441..5364)
                     /locus_tag="PLL59_10950"
     CDS             complement(4441..5364)
                     /locus_tag="PLL59_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMY24368.1"
                     /translation="MKTLFKIIAGIALILFVAFFFIQKKSIPYDTLKEKYKMPSSKFI
                     EIDGMNVHYSIEGEGTPILLIHGTGSVLQTWNAWSAALLKNNFKVIRLDLPAFGLTGP
                     RSDNDYSAKTYVAFLDRFMQKIGIDSFAIAGNSLGGELAWKYAYTFPQKVSKLILVDP
                     AGFYSAEKGNGAIVFKLAKVKWLAQLMSKMDTRILVNRTLEDVYFDDSKISDATREMY
                     YDMSLREGNRESFTARVQQIGNEPVLDVSKIQAPTLTMWGKQDELLDISLLSHFTKIP
                     HHKEIVYDNCGHSPQEEIPEISVKDAIDFLK"
     gene            complement(5493..>6853)
                     /locus_tag="PLL59_10955"
     CDS             complement(5493..>6853)
                     /locus_tag="PLL59_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24369.1"
                     /translation="SCKKEIDFKDEDPLPPFQRPLVELSGNITGERLLDRDTVYRLNG
                     VVNVGIDSIVNITTPQATGILRIEDGTVIIGKKGTSGENPGTLVIHRGSKIYAEGTST
                     NPIIFTSAESTKYPGDWGGLVLCGKATNNQAGGFATLEGSYGAHHGGSDDLDNSGVLR
                     YVRLEYAGFPILTDKEINSLTMGSVGAGTTISHVQASYGLDDAFEWFGGTVNCDHLIA
                     YKTTDDDFDCDFGYRGKVQFAVSFRAYNLADPMSKSNSFEVDNDGGGSTNTPFTAPVF
                     SNVSVFGPKQDSTTSIDANFSRALHLRRSNKIKIYNSFFTGFPQGIVIDGGNTRTYAE
                     NNELVIKNCVLSMLDNWSLFTPYKSDQAGFSVDTWFNDASRNNSTVANITNVGYNTAV
                     LYNNTAPNFLLASTSSLLSGASFTGLSGFTAVNHIGAFDATTNWTNSWTAFNPAAIKY
                     VQ"
BASE COUNT         2117 a         1260 c         1130 g         2346 t
ORIGIN      
        1 tttacttcat aattgctcga agtatttgta aaatatactt cattcaaatg tgcctcttgg
       61 ttcaatgtat tagaaaattc aatcgcgttt atacgcgcat ttccactttg cataatattg
      121 tcattgacat acgctaaaac tggcacttca tatacagctc gattagcttg tatgcgaata
      181 tctttgctta ttttttgagt tgtggtgccg tttgtcacgt gtacatctac tgtgattacg
      241 gaagcaccac tacacgaaaa ttgtataaac tttggaatgc tggcatcttt ctcattataa
      301 gtacatgtaa tcgtgtaagg ctgagctggt ataagcttag tatctacaag atgcgttgtt
      361 ttgtctgcgc tgatatagca caacatactg ctgttgtttg tcgattgcgc ttgtacaatg
      421 gttattgaaa atattgcaag tacacgcgcc atgagtgtta agggtttcaa catggcacta
      481 ggtttttatg tgaggacaaa gttattacgc cttttgatat ttgtcaagac cttttatgca
      541 tttttttcaa tttattttga aaaaaacaat ctttattgct ataaatgagg cacttatgtg
      601 tatttttatt tttgtaaaaa aatttgaaaa tgaatgattt ttatgagaaa gtatatcgtt
      661 tagtggcaaa aattccacga ggaaaagtgt gtacctatgg cgatattgct acatttttag
      721 gtacaaaagg ttctgcgcga accgttggct atgcaatgaa ccacgcccaa agttctactt
      781 tttacgtgcc tgcacatcgt gttgtaaata gaaatggttt gcttacagga aagcaccatt
      841 ttgagtcgcc aacaatgatg caagaattat tagaaagcga aggaataaaa gtaattcaag
      901 atcaaataca agattttgaa aaagtaagat ggattccaaa attgaaaatt aaatagaaaa
      961 aaaattcaaa gcataataaa aaaatccacg ataaacatcg tggattcttt catttgatgg
     1021 ttgtataaac tattattaat tatttagcat cactggcatc accaacatca acaagtcttc
     1081 gttttcgtct ttgtctgttg gacgtattat gcctgcgcga tttggcgtgg acaattcaat
     1141 attaatatcg ctgctgtcta atgctgctaa catttctatt aaaaactttg cgttgaagcc
     1201 aatttccatg gcattacctt gatagtcaca gctcaatttc tctgatgctt cattcgaaaa
     1261 atctaaatct tgcgcagaaa cggtcaactc tgagcctgcc atttttaaca ctacttgatg
     1321 tgttgtttta ttagagtaaa tcgatgttct ttttaaggaa gaaaagaaat cttctttttg
     1381 aacagataat aagttaggat tttcctttgg aatcaccgca ttataatcag gatatttagc
     1441 atcaatcaaa cgacaaatca actgtatatt attgaaacta aaaaatgcgt ttgatttatt
     1501 aaattgtacg gaaacttccg tgtcgttatt ctgcaatact gcttttaata agtttaatgc
     1561 ttttttagga acgatgaatg acgcatttcc gcctttaatt tcgctatggt tatactttac
     1621 caatttgtgc gcatcagtag ccacaaatgt gattccgttg tcatctaatt gaaaatacac
     1681 acctgtcatc gctggtcgca actcatcatt actgacagca aataatgttt ttgaaattcc
     1741 tttattcaag atagatgatg ccaacttcac ttcatctaca ccttcagcag ttggctcttt
     1801 tgggaaatca gcactgtttt cgccacttaa tttatacttg ccattatctg tcgtgatttc
     1861 gatgccattt gtagtttcat caacagcaat cgtaattggt tgctccgcta acgctttcaa
     1921 tgaatctaat aaaatacgag caggaatagc tactttaaaa ctttctttag attccacgtc
     1981 catcgatacc gacattgttg tgtctaaatc tgtagcagtt atgttaagta tatttttatc
     2041 tatatcaaat aaaaaatctt ctaatattgg caatacagta ttcgtgctga ttacaccacc
     2101 tatcttctgt aagtttttaa ataattgtgc tgacgaaacg acaaatctca tacaaagtaa
     2161 tttttttcaa agttaagtaa ttcaatctaa tataaaatgt cttgaaaatc ttatctttcg
     2221 acagattgtt aataagtgct taacgttgtg aataagccaa tatcgatagc attatgaaaa
     2281 ttgccataaa tacaagattt cttctaaaaa acaagataga aggcatcggc atgtttacgc
     2341 aagaaatctt caaacgcgtc gtgcagctca tgcctgagca tgaattttat ttcctatttg
     2401 accgtgcata tgatgaatct tttgtatttg ccaaaaatgt tattccaatc acaatttcac
     2461 cacctgcacg ccatcctgtt ttgtggtata tatggtttga acatgctatt cccaaagttt
     2521 taaaaaaaca ccatatcgat ttatttattt cgccagatgg ttttggctca ttatctactg
     2581 atgttcctca aatcgttacc attcacgact taggttttga acattatccg gaacacacac
     2641 catttttggt gcgtcacttt tatcaatatt ttacacctaa atattgtcaa aaagccagca
     2701 aattaatagc tgtttctaat gccactaaac aagatattat ttcaaaatat ggtgttgatg
     2761 aaaataaaat tagcatagtg tttaatggct ttgaagattg ccgatacatc ataaacaatg
     2821 aacaagcaca aagcttatca attatcgaca agcgcataag tgacaaaaca ccttatttta
     2881 ttttcattgg tgctgtgcat ccacgaaaaa atgtgcttaa tttactaaag gcatttgaac
     2941 tttttaaaca aacatttaca ctcaactatc aattgcttat tgttggaaga aatgcttgga
     3001 tgaatgctga attagaaacg tattaccaac aaatgaaata taaaaatgat gtcatttgga
     3061 tagaaaatat cgaacgtgcg gatttattac aattactaca gaatgcattt gcattatgct
     3121 atccaagttg gtttgaaggc tttggcattc caataataga ggcaatgagc ttaggtgttc
     3181 ctgtaattac atcaaacact tcatctatgg ctgaaatagc agcaaatgct ggcattatgg
     3241 tagcaccaga caatatacaa gaaattgcta ctgccatgca tttacttacg tcagacaatg
     3301 cacttcgtca agacttgatt gacaaaggaa aaatacgtgc taaagaattt gattggaata
     3361 aatctgcact tcaagtggta cagcttatta aaacttatga ataagcaaag ctattacagt
     3421 ttttgtcctt ttaataattt ttcaatatct gcttttactt ttacatattg ttcattcgtt
     3481 aacgatgctt gtaattgttt atctaaatct tgcaatagga ataaaaactc ttttcctgct
     3541 tgttcaggat ttgatttgtg taataatgct atcgcatcac ctctaatttt tccttgtact
     3601 aacaccaaca caactgacat tgtttgtaca tcattcaact ggtattgatg acgtatttta
     3661 tctaagaata tcttttgttc tgcattcaaa tttttacctg ataaaatatt ctcctgcatg
     3721 cctaaggcta acaccaaagt agcaatctgc tggtctgtca tgtgttgttt taactgtggc
     3781 accgtttggc cgttcaacac atctgtctgc tgtttaattt tagctgcatt tccgttatta
     3841 tcagttttaa tttttgtcag ctgcttatca tacgatttta aaataggaat aacttgcgct
     3901 gctttttctg gtgttatttt cataaatgat tgcaactgct caaaaagtgc agtaacattc
     3961 gattgattgc tgatagaagc tggcaacaag ccatttactt ttggtactga tgcgcttgta
     4021 tttgctggag ttgtaggctt atttgttgac ttcgtcagat ctgttttatt tcctggagga
     4081 ttgagttttc cgtccttcac catttgtgtg actttagaaa actgcccatc gttcatgtat
     4141 cctcgaaatt tcagtcctag caacataaac aaggtattgg tattggcgtt aggcttcgca
     4201 ttttcattta aaaacaaaac ataatccgct ttaaattttt taaattgctc tggatttaag
     4261 ttggcttctt tttttacatt ttctgttatt gctgtcaact ctggcgataa attattgaca
     4321 gtgtagtttg tgttttgtgc agatacaagc actactaatc caagaaatac aatggtaagt
     4381 atttttttca tacaaaatat ttattgagtc aaatgtaaca ataagcgtac caaaagaaaa
     4441 tcatttcaga aaatcaattg catctttcac agaaatttct ggaatttctt cttgtggcga
     4501 atggccgcaa ttatcgtaaa caatctcttt atgatgagga atttttgtaa aatgagacag
     4561 caaagagata tccaataatt cgtcttgctt gccccacatt gttaatgttg gtgcttgtat
     4621 ttttgaaaca tctaagactg gctcattgcc tatttgttgc acacgtgctg tgaagctttc
     4681 tctattacct tcgcgcagcg acatatcata gtacatctct ctggtggcat cactaatttt
     4741 actatcatca aaatacacat cttctaaagt acgattaacc aggatacgcg tatccatttt
     4801 actcatcaat tgtgccagcc attttacttt agccagctta aatacaatag caccatttcc
     4861 tttttcagct gaataaaatc ctgcagggtc aactaaaata agtttgctta ctttttgagg
     4921 aaatgtatat gcatacttcc aagccaactc gccaccaagc gaattgccag ctatagcaaa
     4981 actatcaatg cctattttct gcataaatct atctaagaaa gcgacgtaag ttttagcgga
     5041 ataatcatta tcacttctcg gccctgtcaa tccaaatgca ggcaaatcta agcgtatcac
     5101 tttgaaatta tttttaagca aagcagcact ccacgcattc catgtttgca aaacagatcc
     5161 agtgccgtgt attaataaaa ttggagtacc ttcaccttca atgctgtaat gcacattcat
     5221 tccatctatt tcaatgaatt tagaacttgg cattttatat ttttctttga gcgtgtcgta
     5281 tggaatagat ttcttttgta tgaaaaagaa agcgacgaat aatattagcg caatgcctgc
     5341 aataattttg aatagcgttt tcataaaata aaaatacata aaaatatttg ttgcttttat
     5401 cttttaaaat aaaagacctc gaattacttc gaggtcacac acagatgaaa actactattt
     5461 gcttttacac acacgcaaat agcagtagtt ttttattgta catattttat cgcagcagga
     5521 ttgaaggctg tccagctatt tgtccaattt gtagttgcat caaatgcgcc aatgtggtta
     5581 acagcagtga atcctgacaa acctgtaaag cttgcaccag ataacaatga tgaagtagaa
     5641 gccaataaaa aattaggcgc agtattgtta tacaatacag ctgtattata tccaacattt
     5701 gtaatattag ctactgtgct attatttctg ctcgcatcat taaaccatgt atccacacta
     5761 aaacctgctt gatcagattt atatggtgta aataatgacc aattatccaa cattgacaat
     5821 acacaatttt ttatcactag ctcattgttt tctgcatacg ttcttgtgtt gccaccgtca
     5881 attacaatac cttgtggaaa gcccgtaaag aaggaattat aaattttgat tttattacta
     5941 cgacgtaagt gtagcgctct actaaagttc gcatcaatgc ttgttgtaga atcttgtttt
     6001 gggccgaaaa cagaaacgtt tgaaaagaca ggtgcagtaa aaggtgtatt tgttgagcca
     6061 ccgccatcat tatctacttc aaagctattt gatttcgaca taggatcagc taaattataa
     6121 gctctaaaag agacggcaaa ttgcacttta cctctataac cgaaatcgca atcgaaatca
     6181 tcatctgttg ttttataggc aattaagtgg tcgcaattta ctgtgccacc aaaccattca
     6241 aatgcatcgt ctagaccata agatgcttgc acatgactaa tggtagtgcc cgctccaact
     6301 gagcccatcg ttaagctatt tatttcttta tcagtaagga ttggaaagcc tgcatattct
     6361 aaacgaacat atcgtaaaac gcctgaatta tctaaatcat cagagccgcc gtgatgtgcc
     6421 ccataactac cttctaaggt agcgaagccg ccagcttggt tgtttgtcgc tttaccgcac
     6481 aatactaagc caccccaatc accaggatat tttgttgatt cagcagatgt gaaaataatt
     6541 ggattggtag atgtgccttc cgcatatatt ttactgcctc tgtgaattac taatgttcca
     6601 ggattttctc ctgacgtccc ttttttaccg atgataactg taccatcttc aattctaagg
     6661 atgcctgttg cttgtggtgt agttatattt acaattgaat cgatgcctac attgactacg
     6721 ccatttaaac gataaaccgt gtctctatca agcaagcgct ctcctgtgat atttccagac
     6781 aattcaacaa gtggtcgttg aaatggtggc aagggatctt catctttaaa atcgatttct
     6841 tttttacaac ttg
//
LOCUS       DAOQQV010000130         6822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4662_length_6822_cov_8.947392, whole genome shotgun sequence.
ACCESSION   DAOQQV010000130 DAOQQV010000000
VERSION     DAOQQV010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4662_length_6822_cov_8.947392"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLL59_10960"
     CDS             <1..183
                     /locus_tag="PLL59_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24370.1"
                     /translation="APVYLLNESSDFSWLPYIDKRWQGSIPATMVVNGNKNVKAFFET
                     PLEKGQLEFYLKKLGL"
     gene            281..1117
                     /locus_tag="PLL59_10965"
     CDS             281..1117
                     /locus_tag="PLL59_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HMY24371.1"
                     /translation="MSVFERVLGGNVFYEFWKQLQQHIFSEARFIQLITLQPIEIYKA
                     STQLEVDDAKIDLHIKSNVENLMQRRIERTINLLTLNLLENKNKAYIFDFDVIIKAQM
                     AYAEVNKFFNHHFAKQTYVIEENQYYLFIKKFETGNIGTKGEVRLPFMLESKRWFLKR
                     KMHGVAVFNGSINFHQPKYVIKTRNLRYRLETNNWILKIIDRIYHDQIVAFLSDFLQY
                     NFSEELMHAKAEAQAQIDSFQSQTNWIAGQIEEMDMERITIENDGVHAVFLAQGKLRL
                     IP"
     gene            1140..2144
                     /locus_tag="PLL59_10970"
     CDS             1140..2144
                     /locus_tag="PLL59_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY24372.1"
                     /translation="MYKAPLHLSPIENKRILITGGAGFIGSNIAEYLMLHGAKVRVLD
                     NLATGNMSNLDAFQNHPNFEFQEGDIRNIQDCERACKDVDMVCHQAAIGSVPRSIKDP
                     ITTNNVNIGGFVNMLTAAKDAGIKRFVYASSSSVYGDEPNLPKKEERIGNPLSPYAVT
                     KFTNEVYANVFSRCYDIELIGLRYFNIFGPKQDPYGEYAAVMPLFIKSLMQNEAPFIN
                     GDGEQSRDFTYVHNAVQANILGLTTENPATFNQAYNVAFGERFTINQMFDAIADYLKK
                     DIKAIHREPRAGDIRDSLADISKAQNLLSYQAQYSFKQGLPLTIEYFRKVFDKSLAFS
                     "
     gene            2328..3167
                     /locus_tag="PLL59_10975"
     CDS             2328..3167
                     /locus_tag="PLL59_10975"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMY24373.1"
                     /translation="MSKSLQIFCLLLTLLLGAKISYAQENEFGFWAGTSVYVGDLNPT
                     ISFKNARWASGAFYRYNFNNRMTVRAAMNYGFLDADDSRIKRAPFLQQRNLDFKSHIA
                     ELAATFEINFFKYSLIKSRDTKFWTPYIFIGASMFYYNPYTKYDGRKYKLESVGTEGQ
                     KTANRPTKNRGYDQYSFAIPYGIGVKVALNTNWALNVEASSRITFTDYIDDVSGNYAE
                     AEDVNYFVNGVNMGPLLADKSATHVGIPGKQRGTSKDKDRFIFVGVGLTYTIHTSKCP
                     KVL"
     gene            3178..3957
                     /locus_tag="PLL59_10980"
     CDS             3178..3957
                     /locus_tag="PLL59_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HMY24374.1"
                     /translation="MNTNENIAYQDKIDTSKLPEHIAIIMDGNGRWAKAQGKHRIFGH
                     KNGVKAVREVTEGCAEIGIKNLTLYAFSTENWNRPKLEVSALMELLFITMGKEIKTLQ
                     KNNIRLNAIGHLHSLPESNRKVLLELMEATKNNTRMTLTLALSYGAREELTEATKKIA
                     QAVKDGQLSIKDIKQETIMEQLYTKNIPDPELMIRTSGEHRISNFLLWQMAYTELYFT
                     EKFWPEFTKNDLYKAIYEFQNRERRFGMTSEQINEQLTTNN"
     gene            3966..6614
                     /locus_tag="PLL59_10985"
     CDS             3966..6614
                     /locus_tag="PLL59_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HMY24375.1"
                     /translation="MTHTTKNILLLTFGFLFFFYHASAQQIDTTVVNYKEPKKFKLAA
                     LEISGTQFLDKNIIRSLTGLKVGDAITVPGDDITKCIKNLWKQGLFGNVKVFADKFEG
                     ENVYLTIEVLEKPRLNEITLKGLKKSDADDLKKKLEVLKSKPLTDALKLNIRNTIIDQ
                     FEDKSYLNPVINIVEKNDEGLINSVSLVVNVDKGNKVKINHIEFLGREYGELSKMRKV
                     MKDTRERSKVELFLKDGRSRKQKAKDALYTLANLNSNSVKDFFMDRFRITFKGSKFNE
                     RKYEDDKQKLIDYYNNNGFRDAKIVADTVINDDDGNVSIVMNISEGRRYYFGDINFKG
                     NSKFPTETLSRVLNIKKGDVYNAELLQKRLTQDPDGGDISSLYYDDGYLFFSINPVES
                     SVINDSINLEIRINEGPQATIKNVIIEGNDKTNEHVIRRELRTFPGDKFSRSDLIRSQ
                     REIANLGFFDPQETRVEPIPNPSDGTVDIKYTVKEKSADQVELSAGWGGKQGGLIGTL
                     GLKFNNFSLRNIFNKKAWTPLPTGDGQQLTLRIESNGKRYQNYNFSFTEPWLGGKKPN
                     AFTIAAFRSRIQDVSGSTVLGKQITNGLSVSLGTRLKKPDDFFIVQAAANYYGYKLDN
                     FSNRYFVGTQPLDSGKFNNLNFKITLSRNSINQPIFPTGGAKVEASLQFTPPYSLFRK
                     DANYDGMSIAEKYKLIEYHKWKVSIDWYQQLGKSKLVLRTAAKFGFLGTYNKKVGITP
                     FERFQVGGNGIPSNVTLFGTDIIAHRGFEGDYSNSGGDPIYNKFLLELRYPFSLNPSA
                     TIYGLVFFEAANTYASFKDYNPFKLKKSVGIGVRAILPMFGLIGIDYGIRFDSANGSP
                     IKQYNGFFDYIGKNGNISFILGFEPE"
     gene            6639..>6822
                     /locus_tag="PLL59_10990"
     CDS             6639..>6822
                     /locus_tag="PLL59_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMY24376.1"
                     /translation="MKKIILSITFFVAVMMSAHAQRFAYVDTKYILEKLPDYKAAQQK
                     LDDQAALWQKEIDTKNE"
BASE COUNT         2431 a         1150 c         1261 g         1980 t
ORIGIN      
        1 gcacctgtct atctgcttaa cgaaagcagt gatttttcgt ggttgcctta tatcgataag
       61 cgttggcaag gtagcatacc agcaacaatg gttgtcaatg gcaataaaaa tgtaaaagcg
      121 ttttttgaaa caccactgga aaaaggacaa cttgaattct atttgaaaaa attagggctt
      181 taagttgatt tcattcaaat tatgataaaa tctatttaat aaaatattcg caccttaaat
      241 agatatttcc tgctgtcatt atagtaactt tgtgtgctat atgtctgttt ttgaacgtgt
      301 tttaggtggt aatgtttttt acgaattttg gaagcaactc caacagcata ttttttctga
      361 agctcgattc atacagctca tcaccttaca acctattgaa atctataaag cttctactca
      421 gctcgaagtg gacgatgcca aaatagattt gcatattaaa agcaatgtcg aaaatttgat
      481 gcaacgtaga attgagcgta ccatcaatct attaacatta aacttattag aaaacaaaaa
      541 caaggcttat attttcgatt ttgatgtcat tattaaagcg caaatggctt atgctgaagt
      601 gaataaattc ttcaaccatc attttgccaa acaaacctat gtgattgaag aaaatcaata
      661 ttacttattt ataaagaaat ttgaaacggg caatatcggt acaaaaggcg aagtacgact
      721 accatttatg ttagagtcaa aaaggtggtt tttgaaacgc aagatgcatg gcgtagccgt
      781 atttaacggg tctattaatt ttcatcaacc taagtacgtc atcaaaacac gaaatttacg
      841 atatagatta gagacaaata attggatttt gaaaataatc gaccgaatat atcatgatca
      901 aattgtagca ttcttgtctg attttttaca atataatttt tcggaagaat taatgcacgc
      961 aaaagcagaa gcacaagcgc aaattgattc gttccaaagc caaaccaatt ggattgctgg
     1021 gcaaattgaa gaaatggaca tggaaagaat cacaatagaa aatgatggtg tgcacgccgt
     1081 gtttttagca caaggaaaac tgagattaat tccttagatt tgtattttta atacttgaga
     1141 tgtacaaagc accattacac ttatcaccaa ttgaaaataa aagaatactt atcaccggcg
     1201 gtgctggttt tataggttct aatattgcag aatatttaat gttacacggc gcaaaagttc
     1261 gtgtgttaga caatttagct actggaaata tgtctaatct tgatgctttt caaaaccatc
     1321 caaattttga atttcaagaa ggcgatattc gcaatattca agattgtgaa cgcgcttgta
     1381 aagatgtcga tatggtgtgc catcaagcag ccatcggctc cgtgccgcgt tctatcaaag
     1441 atccaattac gacaaacaat gtcaatattg gcggttttgt aaatatgctt acagcagcca
     1501 aagatgctgg catcaaacgt tttgtttatg catcatcatc ttctgtgtat ggcgatgaac
     1561 caaatttacc taaaaaagaa gagcgtatcg gaaatccatt atcgccttat gcggtcacaa
     1621 aatttacaaa tgaagtttat gcaaatgtct tttcgcgttg ctatgatatc gaattaattg
     1681 gcttgagata tttcaatatt tttggtccaa aacaagatcc atacggtgaa tatgccgctg
     1741 tgatgccttt gtttattaaa agtttgatgc aaaacgaggc acctttcatt aatggcgatg
     1801 gcgaacaatc tagagatttt acgtatgttc ataatgcggt acaagccaat atattaggtt
     1861 taactactga aaatcctgcg actttcaatc aagcctataa cgtggcattt ggcgagcgtt
     1921 ttaccattaa tcaaatgttt gatgcaatcg ctgattatct gaaaaaagat ataaaagcaa
     1981 tacacagaga accacgtgct ggcgacattc gcgactcgct ggcagatatt tccaaagcac
     2041 aaaatctttt atcttaccaa gctcaatatt ccttcaaaca aggattgcca ttgaccatag
     2101 aatatttcag aaaagtgttt gataaaagtt tggcattttc ctaattgttc agaaaataat
     2161 ggtcactatt tggcagatta gttctaaatc ggcgatatgc ttgtgataat tgacttaaaa
     2221 aatgtgaatt tttacaaatt actcattaat aaaaccttat aagcaaattc agtgtgcgat
     2281 ttttaatcgt aaaaatgtat ctttgttcct ctttttaaac cactctcttg agcaagagcc
     2341 tgcaaatatt ttgtctgttg ctaaccttac ttttaggtgc gaaaatttct tatgctcaag
     2401 aaaatgaatt tggcttttgg gcaggcacaa gtgtctatgt tggtgatttg aatcctacaa
     2461 tctcatttaa aaatgcgcgc tgggcaagtg gcgcttttta tagatataat tttaacaaca
     2521 gaatgacggt gcgtgcagcg atgaattatg gttttctaga tgctgacgat agcagaataa
     2581 aacgcgcacc atttctacaa caacgcaacc tcgattttaa atcgcatatt gctgaattgg
     2641 ccgcaacatt cgaaattaat tttttcaaat attctctgat aaaatcgaga gatacaaaat
     2701 tttggacgcc atacattttt atcggtgcaa gtatgtttta ttacaatcca tataccaagt
     2761 atgatggcag aaaatataaa ctggaatcag taggcacaga agggcaaaaa acggccaata
     2821 ggccgacaaa aaatcgagga tacgaccaat attcttttgc aattccttac ggaattggtg
     2881 tgaaagtagc attgaatacc aattgggcat taaatgtgga ggccagcagt cgtattacct
     2941 tcacggacta catcgatgat gtgagcggaa actatgcaga agctgaagat gtcaattatt
     3001 ttgtcaacgg tgtaaatatg ggtccattgt tggcagataa atccgctacg catgttggaa
     3061 ttcctggtaa gcaacgcggc accagcaaag ataaagatag atttatattc gtaggtgttg
     3121 gattgaccta taccattcat acatcaaaat gtcctaaagt tttataaaaa atataccttg
     3181 aacacaaacg aaaatatcgc ataccaagat aaaatagata cttcgaaatt gccggagcat
     3241 attgctatta ttatggacgg caatggtcgc tgggcaaaag cacaaggcaa gcatcgcatt
     3301 tttggccaca aaaatggtgt gaaagctgtg cgtgaagtta cagaaggctg tgccgaaata
     3361 ggtataaaaa atctcacgct gtatgctttt tcgacagaaa attggaatcg tccgaaatta
     3421 gaagtgtccg cattgatgga attgttgttt ataacaatgg gaaaggaaat taaaacctta
     3481 cagaaaaaca atattcgttt gaatgcaatt ggccatttac acagcttgcc agaatctaat
     3541 agaaaagtct tattggaatt aatggaagca acgaaaaaca acacgcgtat gactttaact
     3601 ttggcattaa gttatggcgc cagagaagag ctgacagaag ccacaaaaaa aatagcacaa
     3661 gcagtaaaag atggacaatt gtcaataaaa gatattaagc aagaaaccat catggagcaa
     3721 ttatatacaa aaaatattcc tgatccagaa ttgatgatac gcactagtgg cgagcataga
     3781 ataagcaatt ttttgctatg gcaaatggca tatacagagt tatattttac agaaaaattt
     3841 tggcctgagt ttaccaaaaa tgatttgtat aaagcaatat atgaattcca aaatagagaa
     3901 cgacgctttg gaatgacaag tgaacaaatt aatgaacaat tgaccaccaa caactaagaa
     3961 aataaatgac gcacactaca aaaaatattc tactattaac tttcggtttt ttgttctttt
     4021 tctatcatgc atcagcacaa caaatagata cgactgtcgt taattataaa gaaccaaaga
     4081 aatttaaact tgctgcacta gaaatttcag gaacacaatt tctcgacaaa aatattattc
     4141 gctctttgac aggtttgaag gttggcgatg ctattacagt gcctggcgac gatatcacaa
     4201 agtgtattaa aaatttatgg aagcaaggct tattcggaaa cgtaaaagta tttgcagata
     4261 aatttgaagg agaaaatgtg tacttgacaa tcgaagtatt agaaaaacca agattaaatg
     4321 aaatcacatt aaaaggcttg aaaaaaagcg acgctgatga tttaaaaaag aaattagaag
     4381 tattaaaaag caaaccatta accgatgcat taaaactaaa tatcagaaat acaatcatcg
     4441 atcaatttga agataaatca tacctaaatc cagtaattaa tattgtagaa aaaaatgatg
     4501 aaggcttgat aaacagtgtg tcattagttg taaatgtcga taaaggaaat aaagtaaaaa
     4561 tcaatcatat tgaattctta ggtagagaat atggcgagct ctcaaagatg cgtaaagtga
     4621 tgaaagatac acgcgagcgc tcaaaagtcg aattgttttt aaaagatggc agaagcagaa
     4681 aacagaaagc aaaagatgct ttatacacct tggcgaatct caattcaaat tctgtaaaag
     4741 acttttttat ggatagattt agaatcacct ttaaaggttc taaatttaat gaaagaaaat
     4801 acgaagacga taaacaaaaa ctaatcgatt actataacaa caatggtttt cgcgatgcaa
     4861 aaatagtagc agatacagta atcaatgacg atgatggaaa tgtaagtatc gtcatgaata
     4921 tcagcgaagg aagacgctat tacttcggcg atatcaactt taaaggaaat tctaaatttc
     4981 cgacagaaac actatcacgt gtgttaaaca tcaaaaaagg cgatgtgtat aatgctgaat
     5041 tattgcagaa acgattaaca caagaccctg atggcggcga cattagctca ttatattacg
     5101 atgatggtta tttatttttc agtattaatc cagttgagtc atcagttata aacgattcca
     5161 ttaatttaga aattcgcatt aacgaaggac cgcaagcaac catcaaaaat gtaattattg
     5221 aaggcaacga caaaactaat gaacacgtta ttcgacgcga gttgcgcaca ttcccaggcg
     5281 acaaattcag ccgttctgat ttgatacgtt cacaacgtga gattgctaat ttaggatttt
     5341 ttgatccgca agaaacacgt gtagagccaa ttccaaatcc ttcagatggt acagttgata
     5401 ttaaatatac agtgaaagaa aaatcagccg accaagtcga attatcagct ggctggggtg
     5461 gaaagcaagg tggtcttatc ggtactttag gcttgaagtt taacaacttc tctttaagaa
     5521 atatcttcaa taaaaaagct tggacaccat tgccaaccgg cgacggacag cagttgacat
     5581 tgcgtataga aagtaatggt aaacgttatc aaaattataa cttctctttc acagagccat
     5641 ggttaggcgg caaaaaacct aacgcattca caatagccgc tttccgttcg cgtattcaag
     5701 atgtttctgg aagcacagta ctcggcaaac aaatcaccaa tggcctttcg gtatccttgg
     5761 gaacgcgttt gaaaaaacca gatgacttct ttatcgttca agcagccgct aactattatg
     5821 gttataaatt agataatttt tctaatcgct attttgttgg cacacaacca ttagacagcg
     5881 gcaagttcaa caacttgaac tttaaaatca ctttatctag aaattcgata aaccaaccaa
     5941 ttttccctac aggaggcgca aaagtagaag catcattgca gtttacaccg ccatattcct
     6001 tgtttagaaa agatgccaac tacgatggca tgtctattgc cgaaaaatat aaattgatag
     6061 aatatcataa atggaaagtc agcatcgatt ggtaccaaca attaggaaaa tcaaaattag
     6121 tattgcgtac agcggcgaaa tttggattct taggcacgta taacaagaaa gttggcatca
     6181 caccatttga gcgtttccaa gtaggtggaa atggtattcc atccaacgtc actttattcg
     6241 gaacggacat catcgcacat cgtggtttcg aaggcgatta ctctaactca ggtggtgatc
     6301 caatttacaa taaattcttg ctcgagctaa gatatccatt ctctttaaat ccgtcggcaa
     6361 caatatatgg tttagttttc tttgaagcag cgaatactta tgcgagcttt aaagattata
     6421 atccattcaa attaaagaaa agtgttggta taggtgtgcg cgccatcttg ccaatgtttg
     6481 gtctaattgg tattgattat ggcatacgtt ttgatagtgc taatggtagt ccaataaaac
     6541 aatataacgg attctttgac tatatcggta aaaatggaaa tatatcattt atactcggct
     6601 tcgaaccaga ataatggcta aaataaagga caaacaaaat gaaaaaaatt attctatcca
     6661 tcacattttt tgttgcagta atgatgtctg cacatgcgca acgcttcgca tatgtagata
     6721 caaaatatat tctggaaaaa ttaccagatt ataaagcggc gcaacaaaaa ttagacgatc
     6781 aagcggcact ttggcaaaaa gaaattgata ccaaaaatga aa
//
LOCUS       DAOQQV010000131         6667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4851_length_6667_cov_7.470659, whole genome shotgun sequence.
ACCESSION   DAOQQV010000131 DAOQQV010000000
VERSION     DAOQQV010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4851_length_6667_cov_7.470659"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLL59_10995"
     CDS             complement(<1..690)
                     /locus_tag="PLL59_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY24377.1"
                     /translation="MSHFKSPLLSALQKKSRIFLDNIDEDALEEKKYYNRRQFLDTSL
                     KSTLAIGMASSIPLLHSCKNNSTSSATNSSSFKPKVAIIGAGIAGLNAAHHLKKSGAV
                     DYVIYEGLKRCGGRMHTIEEFLPGITLDIGGEFVDTGHAEMLALIEEFKLEKNDYAAD
                     PLHEGENREWYIINGKRYTMKQVFNEFKNFLPIIEKDLASCGENYDTPDAIRLDKLSL
                     DEYLAKIGITGW"
     gene            complement(823..1821)
                     /locus_tag="PLL59_11000"
     CDS             complement(823..1821)
                     /locus_tag="PLL59_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24378.1"
                     /translation="MKKTIFSFFFCFAMTTMSFAAGNANVLITVKLKNGDVVTGKATM
                     PKINVVSPYGNLAIPVEKISNIKFGILSDHTKDGAVLPDLNKLQTVANDNEAKTIYER
                     VLAYGTPVLSTIITFTQNPFYKIADRDNYTIEQLIDELYKKADLSSGQSINDALTFDA
                     SNYIEGAVAFSDITIQAEYGNLTFKREKIESLDLSPIEEASVNADGNYKLKANYHISG
                     NDNNKGWINTGVKVKTGDKFSIIASGKIVLKSLSGGTYNPDGYFSGVKDGAYTDDMAT
                     KYGSLVYKIGEYGDIKQAGTKFESEANNEGNIYISIYETVYDKSNTGSYTVKVTKK"
     gene            complement(1915..2880)
                     /locus_tag="PLL59_11005"
     CDS             complement(1915..2880)
                     /locus_tag="PLL59_11005"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HMY24379.1"
                     /translation="MNIVFPKNTNWFKAALYVAGVYHVLWGLSVIVFPCFWFDLADLS
                     HPNYVQLWQFIGLYEVIFGIGYLMAASNPLRHWRVVLLGFVSKLCVVIGFTYFYSIGQ
                     GPLVVFNMILSNHIFWLIPFLAILYNAYRHQYLLDNEMMHLNSLELPDLLSLYETNKG
                     NNLLSLSQSQPVMLVFLRHFGCVFCKETLSCINQYKQEISNKGTKLVLVHMSDEISAI
                     QSFEKFKLQDVEFVTDPELMLYKGFKLKRGTLLQVLGLKVWIRTIYLFFSKRIFSTIQ
                     EGSNVFQMPGVFLIQDGEICKKFVHQSTADVPPYLELLTVENRHN"
     gene            complement(2940..4214)
                     /gene="hisS"
                     /locus_tag="PLL59_11010"
     CDS             complement(2940..4214)
                     /gene="hisS"
                     /locus_tag="PLL59_11010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMY24380.1"
                     /translation="MINTNPTEGMRDLTPKEVELRSFVANSIVDCYKQYGFTPIETPC
                     VEKINLLTSGDGGENEKMLFKILKRGEKLDVVAATKDSDIVDLGLRYDLTVPLCRFYA
                     NNKNKLPQQFKSIQIGSVWRAERPQKGRFRQFTQCDIDFIGNKEVTAEIQLILATTNA
                     LKNIGFSNFTVRINDRRILENLANFCGFETNSHEKVFIILDKLDKIGIDGVQQELEKS
                     DFPKTAIDKFIQIVNASLQSPLNADDIAKMIPTIPENVLADLKKVMQTVKKQAGDSFS
                     MIFDVSLVRGMGYYTGQIFEIGIQGYSSSIAGGGRYDKMIGKLLNGKEEIPACGFSIG
                     FERVISILEEQHFVVANSRKKIVILYSQEIEDFSLVLAEAAKWRAEGNIVSLEIHNKK
                     NTKKQLEALAQQGFSAFGIYQTEQDFVLKELI"
     gene            complement(4236..4787)
                     /locus_tag="PLL59_11015"
     CDS             complement(4236..4787)
                     /locus_tag="PLL59_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24381.1"
                     /translation="MKYTILFFVFIAFVSCKQDEKKARLSSSKPVYFDIKQIVEHDIY
                     SNTTQNCSEEKSILVNRKSETHINQKVNWREEMQVLLDCDFNKAGWIGKFDSIVSANG
                     LSVSYVTMSSKIPIKKVTINYTEKKQAINSIIIEKKMATFLISNTQQLFYYPNKSFKI
                     IAKQSAIFMQDFNTEIDVKFICK"
     gene            complement(4802..6322)
                     /gene="gpmI"
                     /locus_tag="PLL59_11020"
     CDS             complement(4802..6322)
                     /gene="gpmI"
                     /locus_tag="PLL59_11020"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMY24382.1"
                     /translation="MQQKAMLIIMDGWGINRNPESSAIEAANTPFTDSLYLNYPNTGL
                     LTHGEYVGLPDEQMGNSEVGHMNLGAGRVVYQDLMLINKKVQSGELQKNEVLLKALDY
                     AKANNKNVHFLGLVSDGGIHSHSNHLKALIQLANDRNVANCFVHAFTDGRDTDPKSGR
                     AFIQDIQDFCSNKTTKIASVCGRYFAMDRDKRWERIKKAYDLLVKGVGEKFPSAVEAI
                     DAAYSINCTDEFIEPAVIVDANQIPLTTIQADDVVICFNFRTDRCREITTVLTQQEMP
                     DFGMSTLPLYYVTMTIYDASFKNVKTIFGKDNISNSLGEVIAQHGLTQLRIAETEKYP
                     HVTFFFSGGREEVFQGEERIMIPSPKVATYDLQPEMSAYEIKDAVIRYIDEHTPDFIC
                     LNFANTDMVGHTGVFSAAVKAAEAVDDCVKEVVQKALTHEYAIFLTADHGNADYIVNT
                     DGSPNTAHTKNIVPLFLISNSFNGTLQPGKLADIAPSILKMMQLEIPTDMDGTPLF"
     gene            6413..>6667
                     /locus_tag="PLL59_11025"
     CDS             6413..>6667
                     /locus_tag="PLL59_11025"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HMY24383.1"
                     /translation="MKVLKAIVLFIFCLHFVFTTYGQDVASVIPSALKTANANAIAKN
                     FDKRVDVTIGEVADNYSNEQAEIILKGFLSKFSSRDFIVVH"
BASE COUNT         2047 a         1231 c         1020 g         2369 t
ORIGIN      
        1 ccaacctgtg atgcctattt ttgcgagata ttcgtctaat gataatttat ctaaacgtat
       61 cgcatctggt gtgtcataat tttcgccaca agacgccaag tctttttcaa taattggtaa
      121 gaaatttttg aactcattaa atacctgctt catcgtgtag cgtttgccgt taatgatata
      181 ccattctcta ttttcgcctt cgtgcaaagg atcggcagca tagtcatttt tctctagttt
      241 aaattcttcg attaatgcca acatttccgc atggcctgta tcgacaaact cgccaccaat
      301 gtctaatgtg atgccaggta aaaattcttc gattgtatgc atacgacctc cacagcgttt
      361 taagccttca taaatcacat aatcaactgc tccagatttt ttcaagtgat gtgctgcatt
      421 taatcctgca attccagcgc caatgatagc cactttaggt ttaaaactcg aactattagt
      481 agcagaagaa gtcgaattat ttttacaact atgcagcaat ggaattgagc tcgccatacc
      541 aattgctagt gttgacttca aagaagtatc taaaaattga cgacgattat aatatttttt
      601 ttcttctagt gcatcttcgt caatattgtc taaaaaaatt ctagattttt tttgcaatgc
      661 tgatagcaat ggactcttaa agtgtgacat agttttgttt taggttttgt taaaatacaa
      721 aaaaactatt acagtatgtc attaaataag aaattagcaa taacaattcc aataaaaaag
      781 ccttgaagcg aatgcttcaa ggcagaaaaa tatattgaaa aactatttct ttgtcacctt
      841 taccgtatat gaacctgtgt tcgatttatc ataaacagtt tcataaattg agatgtaaat
      901 atttccttcg ttatttgctt cactttcaaa tttagtacct gcttgtttga tgtcgccgta
      961 ctctcctatt ttataaacaa gcgatccata ctttgttgcc atatcatctg tgtaagcgcc
     1021 atctttaact ccgctaaaat atccatctgg attatatgta ccaccagaca agcttttaag
     1081 tacaattttg ccactggcaa tgatagaaaa cttatcgcct gtttttactt taacacctgt
     1141 attaatccaa cctttattat tgtcattgcc agagatgtga taatttgctt ttagcttata
     1201 atttccatca gcatttacac ttgcttcttc tattggcgac aaatctaagc tttcaatttt
     1261 ttcgcgtttg aatgtaaggt ttccatattc agcttgtata gtaatatctg aaaatgctac
     1321 agcaccttcg atataatttg aagcgtcgaa agtaagcgca tcattgatag attgacctga
     1381 agaaagatct gcttttttat ataattcatc gattaactgc tcgatggtat aattatctct
     1441 atccgcaatt ttataaaatg ggttttgcgt aaaagtgatg atggttgaca acacgggcgt
     1501 gccatacgct aatactcttt cataaattgt ttttgcttca ttatcattgg caaccgtctg
     1561 caacttattt aagtcgggca atacagcacc atcttttgta tgatctgaca gaatgccaaa
     1621 tttgatattg cttatttttt caactggaat ggctaagttg ccataaggag aaactacatt
     1681 tattttaggc atagtagcct tgcctgttac aacgtcgccg tttttgagtt ttacggtaat
     1741 tagtacgttg gcatttcctg ctgcaaacga cattgttgtc attgcgaagc aaaagaaaaa
     1801 agagaagatt gtttttttca taattatttc gattaatgtg ttttaaaaat actcttataa
     1861 aactactttg taaatagaaa aagttccaat taacacaaaa taagatatgg aaatttaatt
     1921 atgtctattt tcgacagtta gcagttctaa atatggtgga acatctgctg ttgattggtg
     1981 tacaaatttc ttacatattt caccatcttg aattaagaaa acacctggca tttgaaatac
     2041 atttgagcct tcttgtattg ttgagaaaat tctcttgcta aaaaaaagat agattgtgcg
     2101 tatccaaact ttgagaccta atacttgtaa taatgtgcct ctttttaatt taaaaccttt
     2161 atacagcatt aattcaggat ctgtgacgaa ttcgacatct tgtagtttga atttctcaaa
     2221 tgattgtatt gctgatattt catcagacat gtgcaccagc accaattttg tacctttatt
     2281 gcttatttct tgcttgtatt ggtttatgca gcttaatgtt tccttacaaa atacacatcc
     2341 aaaatgacgt aagaagacca gcattacagg ttgtgattgt gaaagagata ataaattatt
     2401 gcctttatta gtttcgtaaa gtgataacaa atctggcaat tccaaactat ttagatgcat
     2461 catttcatta tctaataaat attgatgtct gtatgcattg tataaaatcg ctaagaatgg
     2521 aataagccaa aaaatatgat ttgataaaat catattgaac acaactaatg gtccttggcc
     2581 aatggaataa aaataagtaa agccgatgac aacgcatagc ttagacacaa atcctaataa
     2641 cacgacacgc caatgtcgta gtggatttga tgctgccatt aaataaccaa tgccaaatat
     2701 gacttcatac aaaccgataa attgccatag ctgtacataa tttggatgcg ataaatctgc
     2761 taaatcaaac caaaaacaag ggaaaactat aacagataag ccccacaata catggtacac
     2821 gccagccaca tagagtgctg ctttaaacca atttgtattt ttaggaaaaa cgatgttcat
     2881 tagtataatt ccattactac aataactaca tgcagtgtaa attgttcaaa aacttaaaac
     2941 tatattagtt ctttcaacac aaaatcttgc tctgtttgat aaataccaaa tgccgaaaaa
     3001 ccttgttgtg ccaatgcttc caattgtttt ttcgtatttt tcttattgtg aatttctaat
     3061 gaaacaatat tgccttctgc tctccacttt gctgcttccg ctaatactaa tgaaaaatct
     3121 tcgatttctt gactatacag tattacgatt tttttgcgac tatttgctac tacaaaatgt
     3181 tgttcttcca aaatagaaat cacgcgctcg aaaccaatcg aaaaaccaca agccggaatt
     3241 tcttctttgc cattgagtaa ttttccaatc attttatcat atcggccacc tccagcaatt
     3301 gagctgctat agccttgtat tccgatttca aaaatttgac ctgtataata acccatgcct
     3361 cgaactaatg acacatcgaa tatcattgaa aaagaatcgc ctgcttgttt ttttacagtt
     3421 tgcattactt ttttcaaatc agccaataca ttttcaggaa ttgtaggtat catctttgca
     3481 atatcatcag cattcaaagg tgactgtaag gacgcattaa ctatctggat aaatttatca
     3541 atcgccgttt tcggaaaatc agatttttct aattcttgtt gtacaccgtc gatgccaatt
     3601 ttatcgagtt tatctaaaat gataaatact ttttcatgac tatttgtttc aaagccacaa
     3661 aaatttgcca agttttctaa aattcttctg tcgtttatgc gtacggtaaa gtttgaaaat
     3721 ccgatgtttt tcaaggcatt tgttgttgct aatattaatt gtatttctgc tgttacttct
     3781 ttattgccta taaaatcgat atcgcattga gtaaactgtc tgaacctacc tttttgcgga
     3841 cgctcagctc gccaaacact accgatttga attgacttaa attgctgcgg cagtttattt
     3901 ttattgttgg cataaaaacg acacaaaggc actgttaaat cgtatcgcaa tcctaaatct
     3961 actatgtcgc tatcctttgt tgcagccacc acatctaact tttcgccgcg tttcaatatc
     4021 ttgaaaagca ttttttcatt ttcgccacca tcgccacttg tcaacaagtt tattttctct
     4081 acgcatggtg tttctattgg cgtaaatccg tattgtttgt agcaatcaac gatgctattt
     4141 gcaacaaaag aacgtagttc tacttctttt ggtgtaagat ctctcatgcc ttcggtagga
     4201 ttggtattta tcatcatgtt attttctttt cacgcttatt tgcatataaa ttttacatca
     4261 atttctgtat taaaatcttg cataaaaata gcactttgct tagctattat tttaaatgat
     4321 ttatttggat aataaaacag ctgttgtgta tttgaaatta aaaaagtggc catttttttt
     4381 tcaatgatta ttgaattaat agcttgtttt ttttcagtat agtttatagt tactttttta
     4441 atcgggattt tcgaactcat tgttacatag gaaacagaca aaccatttgc agacacaata
     4501 gaatcaaatt ttcctatcca accagcctta ttgaaatcac aatctaacaa cacctgcatt
     4561 tcttcgcgcc agttaacttt ttgattaata tgtgtttctg attttctatt cacaagtatc
     4621 gatttttctt ccgaacaatt ttgagttgta ttagaataaa tatcatgctc cactatttgt
     4681 tttatatcga aataaacagg cttgcttgag gacaaacgtg cttttttctc atcttgttta
     4741 caagaaacaa aagctataaa aacaaaaaac aggattgtgt atttcaagta aataggttta
     4801 gttaaaataa tggtgttccg tccatgtctg ttggaatctc cagctgcatc attttcaata
     4861 tagaaggtgc aatatctgct aattttccag gctgtaaagt gccattaaag ctattgctga
     4921 ttaaaaaaag tggcactata ttttttgtat gtgcagtatt cggcgaacca tctgtattga
     4981 caatataatc agcattacca tggtcggccg tcaaaaagat ggcatactca tgcgttaatg
     5041 ctttttgcac tacttctttc acacaatcat ctacagcctc tgctgcttta acggcagcac
     5101 taaaaacgcc tgtatggcca accatgtctg tgtttgcaaa attcagacaa atgaaatcag
     5161 gtgtatgttc gtcaatatat cttattactg catctttaat ttcgtatgcg ctcatttccg
     5221 gttgcaagtc atacgttgca actttcggcg acggaatcat gatacgttct tcaccttgaa
     5281 aaacctcctc tcttccgcca gaaaagaaaa aggtaacgtg tggatatttt tccgtttcgg
     5341 caatgcgcaa ttgtgtcaaa ccatgttgtg caattacttc gccaagtgaa tttgaaatat
     5401 tgtcttttcc aaatattgtt tttacatttt taaacgaagc atcataaatg gtcattgtca
     5461 cataataaag tggcaatgtg ctcatgccaa aatctggcat ttcttgctgt gtcaatactg
     5521 tagtaatttc tctgcatcga tcggttctaa aattaaaaca aataaccaca tcatctgctt
     5581 ggatagttgt tagtggtatt tggtttgcat caacgattac tgcgggttcg ataaattcat
     5641 ctgtacaatt aattgaataa gcagcatcta tagcctctac agcacttggg aatttttctc
     5701 ctacaccttt caccaataaa tcatacgctt tttttattct ttcccaacgt ttatctctgt
     5761 ccatggcaaa gtagcggcca catacacttg caatttttgt tgttttattc gagcaaaaat
     5821 cttgtatatc ttgtataaaa gctcgtccac tttttgggtc tgtatctcta ccatctgtaa
     5881 atgcatgcac aaaacaattg gctacgtttc tgtcatttgc caattgtatc aatgctttta
     5941 aatgattact atgtgaatga attccaccat cagaaactaa gcctaaaaaa tggacatttt
     6001 tattatttgc tttcgcataa tctaaagcct ttaaaagcac ttcatttttt tgtaattcgc
     6061 cactttgtac ttttttatta atcaacatta aatcttgata aactactcta cctgcaccta
     6121 aattcatgtg gccgacttca gaatttccca tttgttcatc tggcaaacca acatattctc
     6181 catgtgtaag caagcctgta tttgggtaat ttaaatacaa actatctgta aatggcgtat
     6241 ttgcagcttc aattgcacta ctttctgggt ttcgattaat gccccaacca tccataatta
     6301 tcaacattgc tttctgttgc atgcctactt tgttaatttt tattaaaaat aaactatttt
     6361 tagcctttct ttggcacaga ttatgtgcta taaactacga aaatacgatt ttatgaaggt
     6421 attaaaagca atagtgttgt ttatattttg tttacatttc gtatttacga cttatggaca
     6481 agatgtagca agtgtaattc cgtcggcatt aaagacagcc aatgcaaatg ctattgctaa
     6541 gaattttgac aaacgtgttg atgtaaccat tggagaagta gctgataatt acagtaatga
     6601 gcaagcagaa attattctaa aaggattttt atctaaattc tcttcacgag attttattgt
     6661 tgtacat
//
LOCUS       DAOQQV010000132         6587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4944_length_6587_cov_8.550367, whole genome shotgun sequence.
ACCESSION   DAOQQV010000132 DAOQQV010000000
VERSION     DAOQQV010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4944_length_6587_cov_8.550367"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PLL59_11030"
     CDS             <1..947
                     /locus_tag="PLL59_11030"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HMY24384.1"
                     /translation="LQTVADTNEKINIPNTINILTGRKSADITANNLDKLKIFGKGAE
                     KDMVFWNSVVRKAILLNLLEKDIEQYGVLKLTEEGKKFIKKPSSAKIVLNHKFEDGED
                     DDDTIQLNGKAGNVLEPQLLEILKGVRKSVGEKMKLPPYTIFSEQSLEEMATFFPFSV
                     EELSKIHGVNPAKAIKFGNQFLEAIKKYVEENDIERNADFSVIKTADKNSNVKVNIIK
                     SIDKKMPLEHIASSYGLNMEDLITELDSIVHAGTKVNINYYLEDAVDEDIQEEIFDYF
                     MQAENDDIDVAYKKLKAEDIELREIQLVRLKFLSDVAN"
     gene            1295..1630
                     /locus_tag="PLL59_11035"
     CDS             1295..1630
                     /locus_tag="PLL59_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24385.1"
                     /translation="MKKTINTIALFLLIGMAFTSCKKETLPPDLSDTNWVGTMQVGAL
                     IYDSSYLYLNANGTITGKSHKTGGSSYSIDGTWSKTPNSNKVFIHHTIVTAPGTYETV
                     ATLSADELL"
     gene            1746..2339
                     /locus_tag="PLL59_11040"
     CDS             1746..2339
                     /locus_tag="PLL59_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HMY24386.1"
                     /translation="MKKTITAITLFLLIGMAATSCKKESKIIEEHPMQKITTEKSIVD
                     TFHLFSNNNWEFGEKLYFSKNGKITKLGCMMANQGSFRVSLWDFTTKNLIAATTINVT
                     DTTKYTYNSVSPIDVTANTRYVLSINNTSGGEQKLYYIARKRSNLSGNLFPFTSGSVT
                     YEKHHETNSAISVFPLESLSGSYLSGFPDLQFEYSEE"
     regulatory      2464..2629
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2712..3725
                     /locus_tag="PLL59_11045"
     CDS             2712..3725
                     /locus_tag="PLL59_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HMY24387.1"
                     /translation="MTKDLYQIAQERILILDGAMGTMIQRYGLQEEDFRGERWKDHSH
                     PLKGNNDLLSITRPDIIKEIHRQYFEAGADIVETNTFSGTTIAQADYKLEDAVYDINY
                     YSAKIAKEVADEMTAKEPHKPRFVAGAIGPTNRTCSISPDVNDPGFRAVTFDELVKAY
                     YDQTKALIDGGADILLIETIFDTLNAKAALFAVQTYFEDTGKEYPIMISGTITDASGR
                     TLSGQTTEAFLISMSHVPLFSIGLNCALGAKELRPYLQVLAKEAPFYVSAYPNAGLPN
                     AFGGYDETPEQMAEATREYLELGLINILGGCCGTTPDHIRCFAEVAKNHKPREVHQVQ
                     AVQ"
     gene            3744..6443
                     /gene="metH"
                     /locus_tag="PLL59_11050"
     CDS             3744..6443
                     /gene="metH"
                     /locus_tag="PLL59_11050"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HMY24388.1"
                     /translation="MEIDCSVLKLSGLEPLIVTKASNFINIGERTNVTGSKVFLKMIK
                     EERFEDALSVAMDQVRGGAQVLDVNMDEGMIDGEKAMVKFLNLIASEPEIAKIPIMID
                     SSKWEIIEAGLKCVQGKSIVNSISLKGGEEEFIRQAKLIKKYGAATVVMAFDEDGQAD
                     SYERRIEICERSYRVLVDKVKFNPNDIIFDPNIFPVATGMEEHRNNAMDFFRATNWIR
                     TNLKGAHVSGGVSNVSFSFRGNNVVREAMHSAFLYHAIKEGMDMGIVNPSMLEVYDDI
                     NKELLERVEDVLLNRREDATERLLEFADTVKGTAKKKEEDLSWRNQDVNERLKYALVK
                     GIVDYIEADTEECRLQFDKPLSVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKK
                     SVAYLLPFIEDSKQAGDSNKNGKILLATVKGDVHDIGKNIVGVVLACNNFDIVDLGVM
                     VPCDKILDAAEKEGADIIGLSGLITPSLDEMVFVAKEMKRRGMKQPLLIGGATTSKIH
                     TAVKIEPNYNQPTVHVLDASRSVPVASSLLNPNNKEKFEAEIKAEYEMMRQRHLSKQV
                     IKDYLTIEDARKNKLQLDWENYTPPKPTFLGVKVFDNYDLNKLRAYLDWTPFFSTWEL
                     HGKYPNILEDKMVGTEAKKLFADANALLDKIIAEKWLTARGVIGFFEANSNVDDIELK
                     DISKEETLTDKRKSVLHHLRQQNKKGEGLPNYCLSDFVAPKESNKQDYIGGFAVTTGI
                     GIEKWIQYFQDTHDDYNKIMLAALADRLAEAFAEHMHERVRKEFWKYDTTESLTNEDL
                     IKEKYKGIRPAPGYPACPDHTEKPMLFKLLEAEKNAGIMLTESNAMYPASSVSGWYFS
                     HPDSKYFGVGKIAKDQLEDYAKRKNMQTEDMERWLQSVLNYDA"
BASE COUNT         2387 a         1058 c         1251 g         1891 t
ORIGIN      
        1 ccttacaaac agttgccgat accaacgaaa aaataaatat tcctaatacc attaatatac
       61 ttactggtcg caaatcggct gacattacag ccaacaactt agacaaatta aaaatatttg
      121 gaaaaggagc ggaaaaggat atggtatttt ggaattctgt tgttcgcaaa gcaatactac
      181 taaacttgct ggaaaaagat atcgaacagt atggcgtctt gaaactaaca gaagaaggca
      241 agaaatttat aaaaaaacca agctcagcaa aaatagtatt gaaccataaa tttgaagatg
      301 gcgaagatga tgatgatacc atacaactca acgggaaagc aggcaatgtg ttagaaccac
      361 aattgttaga aatactaaaa ggtgtacgaa aatctgtagg cgaaaaaatg aaattgccgc
      421 catacaccat tttttcagag caatcattgg aagaaatggc cacttttttc cctttttctg
      481 tggaagaatt atcaaaaata catggtgtaa atccagcgaa agcaataaaa tttggcaatc
      541 aatttctcga agcaataaaa aaatatgtcg aagaaaatga tattgaacgc aatgctgatt
      601 tctctgtgat aaaaacagca gacaaaaatt caaatgtaaa agtaaacatc atcaaatcta
      661 tcgataaaaa aatgccatta gaacacattg ctagttcata cggtttaaat atggaagatt
      721 tgattacaga attagatagc attgtacatg caggcacgaa agtaaatatc aactattact
      781 tagaagacgc tgttgatgaa gatattcaag aagaaatttt tgactatttc atgcaagcag
      841 aaaatgatga tattgatgta gcatataaaa agcttaaagc agaagatatc gagctccgcg
      901 aaattcaatt agtccgttta aaatttttaa gcgacgtagc gaattaaacc cacaatttta
      961 taattatttt attggaaaac tacggcaaac tacttgctat acttgctagt ttgcgccaat
     1021 tgttttttgt aagttgaaat atacaaatca aactattgtt gcaatttcac aaagcatact
     1081 tcataaaata gcatctttcc actactgata tagaagcgta aaacagcttt tttcgtaagt
     1141 agaattcctt ttttttcttt tatgtaggaa atcaagttga aacataattg tatttaaaat
     1201 gaacgaattc taagtgaaaa ccgccaattg ataaatgttt attgactatt ttaaataaaa
     1261 cagtcaaatt tgagctaata aaaaacaaaa tactatgaaa aagacaatca acacaatcgc
     1321 tttattttta ctaattggta tggcttttac atcatgtaaa aaagaaactc ttccaccgga
     1381 tttgtcagat actaattggg taggtacaat gcaggtaggt gctttaattt atgacagctc
     1441 ctatttatac cttaatgcaa acggtactat tactggcaaa tcacataaaa caggtggttc
     1501 tagttattca atagatggaa cgtggagtaa aacgccgaat agcaacaaag tgtttataca
     1561 ccatacgatt gtaacagcac ctggcactta tgaaactgta gcaacgttaa gtgctgatga
     1621 acttctttaa aaataatttc ttaagaagac acattatcta aataaaaatt tatacatttg
     1681 tacaaacaat aaaaaataca aaacaataaa tgaaatatca atttaagaac aaaaaacaaa
     1741 atacaatgaa aaagacaatc acagcaatca ctttattttt attaattggc atggccgcaa
     1801 catcatgtaa aaaagaaagt aaaattattg aagagcatcc aatgcaaaaa attaccactg
     1861 aaaaaagtat tgtagataca ttccatttgt tttcaaataa caattgggag tttggagaga
     1921 aactttactt ttctaaaaat ggtaaaatta ccaaattggg ctgtatgatg gcaaaccaag
     1981 gaagttttag agtttcttta tgggatttta ctactaaaaa tttaattgca gctactacaa
     2041 ttaatgttac tgataccaca aaatatacgt acaatagcgt ttcacctata gatgttacag
     2101 ctaatactag atatgtgtta tctatcaata acacaagtgg aggtgaacaa aaattatatt
     2161 atattgcacg taagcgatct aatctgtcag gtaatctttt tccatttact agtggtagtg
     2221 taacttatga aaaacatcac gaaactaatt cagctatttc cgtctttcct ttagagtcgc
     2281 tttctggtag ttacttgtct ggttttccag acttgcaatt tgaatattca gaagaataac
     2341 gaaatcataa atttttttat acaggctgtc attttttgac agcctttttt attgccctat
     2401 ggcaatttgt tttataatgt gcaattagta tctttgtgcc tgtaaaagtg ttcttttatt
     2461 cataacttat tacgaaagac ggagagaata ggctctatga tgtcttggca accttttaga
     2521 tgaaaagata tgagatgtga gaaataagat ttctcctctc tttaaatctc atttctcctc
     2581 tctaaaaaag gtgccaaaac ctactttgcg cagcaaagaa cgataagtaa gcaaaatcct
     2641 gtcttttttc ttattgtttt tcgtaattcc ttgcttaaat gaactttatt ttcatggaat
     2701 aataaacgat aatgaccaaa gatttatacc aaattgctca agaacgcatc cttattttag
     2761 atggcgccat gggcaccatg atacaacgtt atggcttgca agaagaagat tttcgtggcg
     2821 agcgctggaa agaccactca catcctttga aaggaaacaa tgacctcttg tctatcacac
     2881 ggccagatat catcaaagaa attcatcgtc agtattttga agcaggtgct gacattgtag
     2941 aaaccaatac atttagcggc accaccattg cgcaagctga ttataaactc gaagatgccg
     3001 tgtatgatat caactattat agtgcaaaaa ttgcaaaaga agttgccgat gaaatgacag
     3061 caaaagaacc acataaacca cgctttgtag caggcgctat tggaccgaca aaccgtacct
     3121 gttccatctc gccagatgtt aatgatccag gatttagagc agtcactttc gacgaattgg
     3181 tgaaagcata ctacgaccaa accaaagcac tcatagatgg tggtgccgat attttattaa
     3241 tagaaactat tttcgatact ttaaatgcta aagcagcatt atttgcagta caaacttatt
     3301 ttgaagatac gggaaaagaa tatccaatta tgatttctgg caccattaca gatgctagtg
     3361 gccgaacact cagcggacaa acaacagaag catttttgat ttctatgtcg cacgttccct
     3421 tattttctat cggattaaat tgtgcattag gagcaaaaga attaagacca tatttgcaag
     3481 tgctggccaa agaagcacca ttttatgtaa gtgcatatcc gaatgctggt ttaccaaatg
     3541 catttggcgg ctacgacgaa acgccagaac aaatggcaga agctacacgt gaatatttag
     3601 aattaggatt gataaatatt ctcggcggtt gctgtggcac tacgccagac catatcagat
     3661 gttttgcgga agttgcaaaa aatcacaaac caagagaagt acatcaggta caagcagttc
     3721 aataagtaaa aaataaaaaa taaatggaaa tagattgtag tgttttaaag ctttcaggat
     3781 tagagccact cattgttacc aaagcttcta acttcattaa tattggcgaa agaaccaatg
     3841 ttacaggttc taaagttttt ctaaaaatga taaaagaaga acgctttgaa gacgcacttt
     3901 ccgtagcgat ggatcaggtg cgtggcggtg cgcaagtact tgacgttaac atggacgaag
     3961 gtatgattga cggtgaaaaa gccatggtaa aatttttaaa cctaatagca tcagaaccgg
     4021 aaattgcgaa aataccaatc atgatagatt cttcgaaatg ggaaattatt gaggctggtt
     4081 tgaaatgcgt acaaggaaaa tctattgtca attccatttc tttaaaaggt ggcgaagaag
     4141 agtttattcg ccaggctaaa ttgataaaaa aatatggcgc agcaacggtg gttatggcat
     4201 ttgacgaaga cgggcaagcc gacagctatg aacgcagaat cgaaatttgt gaaagaagtt
     4261 atcgagtgct ggtggacaaa gtaaagttca atccaaacga tataattttc gatccaaata
     4321 tatttccagt tgctaccggc atggaagaac atcgcaacaa tgcgatggat ttttttcgtg
     4381 ctaccaattg gatacgcact aacttaaaag gagcacatgt gagcggtggt gtttccaatg
     4441 tgtctttttc ttttagagga aataatgtgg tgagagaagc tatgcactca gcatttttat
     4501 atcatgctat aaaagaaggc atggatatgg gcatcgtaaa tcctagcatg ttggaagtgt
     4561 atgacgacat caacaaagag ttattagaac gcgtggaaga tgttctatta aacagaagag
     4621 aagatgcaac agagcgttta ttagagtttg cagatacagt aaaaggtact gcaaagaaaa
     4681 aagaagaaga tttaagctgg agaaatcaag atgtcaacga gcgtttaaaa tatgcgttgg
     4741 taaaaggtat tgtagattat attgaagcag atacagaaga atgtcgatta caattcgaca
     4801 agcctttaag tgttattgaa ggtccattaa tggacggtat gaacgttgtt ggcgatttat
     4861 ttggcgctgg aaaaatgttc ttgccacaag tcgtaaaatc ggctcgtgtg atgaaaaaat
     4921 cagtagctta tttattgcca tttattgaag actcaaaaca agcaggtgac agtaataaaa
     4981 atggaaaaat tttattagcc acagtaaaag gcgatgtgca tgatatcggt aaaaatattg
     5041 taggcgttgt attggcgtgt aataattttg atatcgtcga tttaggcgtt atggtgccat
     5101 gcgataaaat attagatgct gctgaaaaag aaggtgctga tatcatagga ctgagtggat
     5161 tgattacgcc atcattagat gaaatggtgt ttgtggcaaa agaaatgaaa cgccgtggca
     5221 tgaaacaacc tttattaatt ggtggtgcca cgacttccaa aattcataca gctgtaaaaa
     5281 ttgaacctaa ttacaaccaa cccaccgtgc atgttttaga tgcatcacgc agtgtgccgg
     5341 tagcaagttc tttattaaat cctaataata aagaaaagtt tgaagctgaa attaaagcag
     5401 aatatgaaat gatgcgccag cgccatctga gtaaacaagt gataaaagat tatttaacta
     5461 tcgaagatgc acgtaaaaac aaactgcaat tagattggga aaattacaca ccaccaaagc
     5521 caactttctt aggtgtgaaa gtatttgata attatgattt gaataaactt agagcgtatt
     5581 tagattggac tccattcttt tctacttggg aattgcatgg taaatatcca aacattttag
     5641 aagataaaat ggtaggcact gaagccaaga aattatttgc cgatgcaaat gcattgctcg
     5701 ataaaatcat tgcagaaaaa tggttgacag caagaggcgt gattggattt tttgaagcca
     5761 attctaatgt cgatgatatt gaattaaaag atatcagcaa ggaagaaaca ttaacagata
     5821 agcgcaaatc agtgttgcac cacctgcgtc aacaaaataa aaaaggagaa ggtttgccta
     5881 attattgttt aagcgatttt gtagcgccaa aagaaagcaa taaacaagat tatatcggcg
     5941 gatttgcggt aactacaggt attggtatcg aaaaatggat tcagtatttt caggatacac
     6001 acgatgatta taacaaaatt atgttggcag cattagccga ccgtttggct gaggcatttg
     6061 ccgaacacat gcacgagcgc gtgcgtaaag aattctggaa atacgatacc acagaatcat
     6121 tgacaaatga agatttaatt aaagaaaaat acaaaggcat acgtcctgcg ccaggctatc
     6181 ctgcttgtcc tgaccatacc gaaaaaccga tgttgtttaa gttattagaa gcagaaaaaa
     6241 atgcaggaat catgctaaca gaaagcaatg ccatgtatcc agcatcgagt gtgagtggtt
     6301 ggtatttctc gcatccagac agtaaatatt ttggtgttgg aaaaattgcg aaagatcaat
     6361 tggaagatta tgccaaaagg aaaaatatgc aaaccgaaga catggaacgc tggctgcaaa
     6421 gcgtgttgaa ttatgatgct taaattatat caccattctt tattctatat aattaatttt
     6481 tgatttctct gtcttgtttc gtgtcctcac gaaacaaaac atagttcgtg gagacaccat
     6541 aagtgttcgg aagattcaca tactctgaat aaatagataa gctgtat
//
LOCUS       DAOQQV010000133         6563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_4969_length_6563_cov_7.017978, whole genome shotgun sequence.
ACCESSION   DAOQQV010000133 DAOQQV010000000
VERSION     DAOQQV010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4969_length_6563_cov_7.017978"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..710)
                     /locus_tag="PLL59_11055"
     CDS             complement(75..710)
                     /locus_tag="PLL59_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMY24389.1"
                     /translation="MNQRPFIIGISGGSGSGKTTFVNELAKSFSTDQVCILSQDNYYK
                     PREEQIIDNAGEKNFDLPESFREAEYHNDVLRLLNGEDVVLKEYVYNNPLAAPKLITY
                     KSAPIVIIEGIFVFHFKDVSDLMDFKIFIDADEHIKLIRRIKRDQIERNYPIEDVLYR
                     YQNHVFPSYQKFILPYKPACDIVVNNNVNFENALNTVKLSIKEKLDGWKQN"
     gene            783..1694
                     /gene="folD"
                     /locus_tag="PLL59_11060"
     CDS             783..1694
                     /gene="folD"
                     /locus_tag="PLL59_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HMY24390.1"
                     /translation="MQILDGKKLSAEIKAEIADTVKKMVLSGKKAPHLAAILVGNDGA
                     SRTYVASKVKSCEEVGFKSTLIRLESDITQNELITQINQLNNDDSIDGFIVQLPLPKH
                     IDEEKILLAIAPEKDVDGFHPMNVGRMTLHLPSYISATPKGILEMLNRYNIETEGKHC
                     VVIGRSNIVGLPMSLLMQRRSQPGNCTVTICHSKTNDLSKYTLEADIIIAALGKPKFL
                     TADMVKPGAVVIDVGITRVDDASTKSGFRLYGDVDFENVSKKCSFISPVPGGVGLMTV
                     ASLLQNTLSAANEEFYKKTIDFSVDNI"
     gene            complement(1714..2481)
                     /gene="cysQ"
                     /locus_tag="PLL59_11065"
     CDS             complement(1714..2481)
                     /gene="cysQ"
                     /locus_tag="PLL59_11065"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HMY24391.1"
                     /translation="MIQEVIKIAIQAGDAILDIYKQDFDVEIKSDQSPLTQADKNANK
                     IIVAGLQRLDKNIPIISEENKLTDYSIRKNWTTCWMVDPLDGTKEFIKKNGEFTVNIA
                     LIQNGIPFLGVVHVPAQNITYFAVKGKGSFKIKDAETTKLAIRPLAADGTLKIVGSRS
                     HQTPELLNYVAQQQSKFEKVDFVAAGSSLKFCLLADGMADVYPRLGPTMEWDTCAGQI
                     VATEAGAIVLQFDNNEPLLYNRENLLNPYFIVKHPDL"
     gene            complement(2491..3039)
                     /gene="rfbC"
                     /locus_tag="PLL59_11070"
     CDS             complement(2491..3039)
                     /gene="rfbC"
                     /locus_tag="PLL59_11070"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HMY24392.1"
                     /translation="MKVITTPINDLFVIEPSVFADNRGFFYESYNAEKLKAFGINYEF
                     VQDNHSKSSYGVLRGLHYQNNPYAQTKLVRVTHGKVLDVAVDIRRNSSTYLQHFSIEL
                     SAENKLQLLIPPGFAHGFVVLSETCEFLYKCDNYYNKSAEGGIAYNDSSLQIDWRIPA
                     NDIVLSEKDQLNPTVEHANFNF"
     gene            complement(3052..4140)
                     /locus_tag="PLL59_11075"
     CDS             complement(3052..4140)
                     /locus_tag="PLL59_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24393.1"
                     /translation="MQENEEINPKENELSEELADVNQEEGDQGLKQFLVIVKEIFGIA
                     KSYALLFVIPIILTGLYGYYKGSLVKPTYTAKVTFFLSEERPQLPGTTNFANLLQTTN
                     TNFGNPKKLKEYAFTEKVGSKMLFKKQVFRGREDYLANHYLRLTSGYKESYFRDFTTV
                     SDMSIPDYLVFKKILKNIREMVTVDYNEAEIYSITATTGDEEFSKLLCDCFYNNLIDY
                     YIDRATRKAKITVDFLEEKLAYVKAQLENSEFNLADYKDKANNLVTFKADLNETRYLR
                     NKALLEALYSMTATALEGSKTNYQSIMPLFQTIDEPHLPLQKNVVSTRKQFIMFMLIG
                     VFIDIIIIIGIYIRRHFWSSIKETIKAA"
     gene            complement(4142..>6563)
                     /locus_tag="PLL59_11080"
     CDS             complement(4142..>6563)
                     /locus_tag="PLL59_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HMY24394.1"
                     /translation="LKEAQKASETVEKITATATRELREKLDTVDIYGFTYFRQKDVKI
                     FNNAVDIKPPSNYILGVGDQLVVSIWGYADYNRLFYIDKDGYIQQENIGRVYLKGLTL
                     EQAKGLLRTRFANAYMIDKSDFDVSINYSRVITINVVGDVENPGSYTFPAINTAYNIL
                     AYVGGPSKSGSIRDIQIKRDGKIIRNFDLYKFLFTPEKQDDIFLDNNDYIYVPAQKNI
                     VRIEGAINREGKYEMKNGETFEDLLKYCGGYKPDAFTKVIQIRRFDENKQILIDYNLD
                     SVRLSGGKILLKHGDVVGVRSIPEKVKNYNDINGSVLLPGRYEWREGDRVWDLISKAQ
                     GLKEDAYLDEAYVIRTNYGDFRKHTYKVSLQNIIDDKNSEDNMLLTFRDVVEVYAKTD
                     YFDKFDIEVKGEVRKPRRFESEKGMSLRDALIMCNGLKEEALLDKIIIYRVNDDLTEK
                     LISFRIDTTNNFEALDTFKLKKRDKIYVLKDLRRIDKYYIEVNGMVRKNDTFPYMESM
                     TLADALILAQGFKMEAASNRIEIARIANYDKAISQSVPTQVTILNYSISKNIGKDPVA
                     NSVKLQPFDQIYVRPTPEFEYQMKVTVKGEVLYPGTYVLTSKDEKLASLIERAGGLTR
                     FAYADGAKLKRTEDGIGNILTNLNVAMKNKNSKFNYVLKEGDEIDIPAIKDLVALKGE
                     FNYPADEKPENLYVPFNEGKDAKFYIYKYGAGFSDSAYKKKTYVIRPNGQILSTQRTF
                     FGKNYPKVEKGSTIVIPKRIPKEKKQRVRNANSGAILQKAVTGAVGAISSVLTLYLLF
                     ETVRNTKK"
BASE COUNT         2057 a         1163 c         1035 g         2308 t
ORIGIN      
        1 agcacattgc cttattccaa atacatgaaa ggcttataca atcgtacgtt tactttctct
       61 attaattttg aaggttaatt ttgtttccag ccatctaatt tttctttaat agaaagcttc
      121 accgtattga gtgcattttc gaaattgaca ttgttattta caacaatatc gcaagcaggt
      181 ttataaggca aaataaattt ttggtatgat ggaaacacgt gattttggta gcgatataat
      241 acatcttcaa tcggatagtt tcgctctatt tgatcgcgtt ttattctacg aataagtttg
      301 atatgttcgt cggcatcaat aaatatttta aaatccatca aatcagatac gtctttaaaa
      361 tgaaacacaa atatgccttc gattattaca attggagccg acttataagt aatcaatttt
      421 ggagctgcca atggattgtt gtacacatat tctttcaaaa ctacatcttc gccatttagc
      481 aagcgcaata catcattatg gtattctgct tctctaaaag attcaggtaa gtcaaaattt
      541 ttttctcctg cattatcaat tatttgctct tcacgtggtt tgtaataatt atcttgactt
      601 aaaatacaaa cttgatctgt cgaaaagctc tttgccaatt catttacaaa tgtggttttt
      661 ccgctgccac ttccacctga aatgccaata ataaatggtc gttgattcat ctttttaaaa
      721 aaaatattct ccaaaactaa gcattcgaaa ttatcttacc tatttttatg caaaatttcg
      781 tcatgcaaat attagatggc aaaaaattat cagctgaaat aaaggctgaa attgccgata
      841 cagtaaaaaa aatggtgttg agtggaaaaa aagcacctca tttagcggca attttagtag
      901 gaaatgatgg tgcaagtcga acttatgtag ctagtaaagt aaaatcttgt gaggaagtag
      961 gatttaagtc cacactgatt agattggaaa gtgatatcac acaaaacgag ctgattacac
     1021 aaataaacca gctcaacaac gatgattcta ttgatggatt tattgttcaa ttgccattac
     1081 ctaaacatat tgacgaggaa aaaattctgc tcgcaattgc gccagaaaaa gatgttgacg
     1141 gatttcatcc gatgaatgta ggccgtatga cattgcacct gccgagttat atttctgcca
     1201 cgccaaaagg aattttagaa atgctgaaca gatataatat tgaaacggaa ggaaaacatt
     1261 gcgtagttat cggcagaagt aatattgtgg gcttgccaat gagtttatta atgcagcgac
     1321 gaagccagcc tggcaattgc acggtaacga tttgccatag caaaactaat gatttgtcta
     1381 aatacacatt agaagcggat attataatag ctgcacttgg caaacctaag tttttaacag
     1441 cggatatggt aaagccaggc gctgttgtaa ttgacgtagg tattacacgt gtagatgatg
     1501 ccagcacaaa atcaggattt cgcttatatg gcgatgttga ctttgaaaat gtaagtaaga
     1561 aatgtagttt tatttcgcca gttcctggtg gcgtaggttt aatgacggtg gcttcattat
     1621 tacaaaatac acttagtgct gcaaacgaag aattttacaa aaaaacgata gacttttctg
     1681 tcgataatat ataagacagt caaaaaaact ggtttataaa tctggatgtt ttacaataaa
     1741 ataaggattc aacaggtttt ctctattata caatagtggt tcattattat cgaattggag
     1801 aacaatggcg ccagcttctg ttgctacgat ttgtccagcg caagtatccc attccatagt
     1861 aggtccaaga cgtggataga catcagccat accgtctgct aatagacaaa acttaagtga
     1921 actacctgct gcaacaaaat caactttttc aaattttgat tgttgctgcg ctacataatt
     1981 taataattca ggagtttggt gcgaacgcga gcctacaatt ttcaaagtac catcagcagc
     2041 taaaggtcta atcgccagct tagtcgtttc agcatcttta attttaaaac taccttttcc
     2101 ttttacagca aaataggtaa tgttttgggc tggcacatgc acaacaccaa ggaatggaat
     2161 gccattttgt attaaagcta tatttacagt aaactctcca tttttcttaa taaattcttt
     2221 agtgccatca agcggatcaa ccatccaaca tgtcgtccaa ttttttcgaa tactataatc
     2281 ggttagctta ttctcttcag aaatgattgg aatattttta tctaatctct gcaaacctgc
     2341 aacaataatt ttatttgcat ttttatcagc ttgagttaat ggcgactgat cgctttttat
     2401 ttccacatca aaatcttgtt tatagatatc taaaatagca tctcctgctt gaatggcaat
     2461 tttaattact tcttgaatca tgataaaaaa ttaaaaattg aaattagcgt gttcaacagt
     2521 cggattgagc tgatctttct cacttaagac aatatcattt gcaggaattc tccagtcgat
     2581 ttgcaaggaa gaatcattat aagcaattcc accttcagca gatttattgt agtaattatc
     2641 gcatttatat aagaattcac aagtttcaga tagcactaca aagccatgcg caaagcctgg
     2701 tggaataagc aattgaagtt tattttctgc tgataattca atagaaaaat gttgcaaata
     2761 tgttgacgaa tttcttctaa tatctaccgc aacatctaac acttttccat gtgttacacg
     2821 cactaatttt gtttgtgcat aaggattatt ttgataatgc aaaccacgca agacgccata
     2881 acttgatttg gagtgattat cttggacaaa ctcgtaatta ataccgaatg ctttaagttt
     2941 ttcggcatta taactttcgt agaaaaaacc tctattgtca gcaaaaacac ttggttctat
     3001 tacaaacaaa tcgtttattg gtgtagtaat gactttcatg ataataataa gctacgctgc
     3061 ttttatcgtc tctttgattg atgaccaaaa atgacgtctg atgtaaatac cgataatgat
     3121 gatgatatca atgaatacac caataagcat aaacataata aattgcttac gtgtggatac
     3181 tacatttttt tgtagtggta aatgtggttc atcaattgtt tggaataaag gcatgataga
     3241 ttgataattc gtcttagaac cttctagtgc tgttgcagtc atggaatata atgcttctaa
     3301 caaagcttta tttctaagat atcttgtttc attcaaatcg gctttaaagg taaccaagtt
     3361 attggcttta tctttataat ctgctaaatt aaactcagag ttttccaatt gagcttttac
     3421 gtatgctaat ttctcttcca agaagtcaac agtaattttt gctttacgag ttgcacgatc
     3481 aatataatag tcgattaagt tattatagaa acaatcacac aataatttcg aaaattcttc
     3541 atcgccagtg gttgctgtaa tagaataaat ttcagcttca ttataatcaa cagttaccat
     3601 ttctctgata ttttttagta tttttttaaa cactaaataa tctggaatgc tcatatcgct
     3661 aaccgtagta aaatcgcgaa agtagctttc tttatagcct gatgttaatc tcaaataatg
     3721 atttgccaaa taatcttctc ttcctctgaa cacttgtttt ttaaacaaca ttttagaacc
     3781 aactttttca gtaaaagcgt attcttttaa ttttttggga tttccgaagt tggtatttgt
     3841 tgtctgtaat aagttagcaa aattagttgt accaggcaat tgtggacgct cttctgagag
     3901 gaagaaagtt acttttgctg tataagtagg tttcactaaa gagcctttat agtaaccata
     3961 caaaccagta agaataattg gaataacaaa caagagagca tagcttttag cgatgccaaa
     4021 tatttctttg acaattacta agaattgctt aagtccttga tctccttctt cctgatttac
     4081 gtcagcaagt tcttcgctca attcattttc tttaggattt atttcttcgt tctcttgcat
     4141 attatttttt agtattccgc actgtttcaa agagtaaata taaagttagt acagaagaaa
     4201 ttgcacctac agcgcctgtg accgcttttt gcaatattgc gccactattg gcattacgga
     4261 ctctttgttt tttctctttt ggtatgcgtt taggaattac gatagtcgaa cctttttcca
     4321 cttttggata attttttcca aagaatgttc tctgcgtgct taatatttgg ccatttggtc
     4381 gaataacata ggttttcttt ttgtatgcac tatcactgaa tcctgcgcca tatttataaa
     4441 tatagaattt tgcatcttta ccttcgttga atggcacgta tagattttca ggtttttcat
     4501 ctgctggata attaaattct ccttttaaag cgactaagtc ttttattgct ggaatgtcta
     4561 tttcgtcgcc ttcttttaaa acgtaattaa acttagagtt tttgtttttc atcgcaacat
     4621 ttaagttcgt caaaatattt ccgataccat cttctgtgcg ttttagtttt gcgccatcag
     4681 cataagcaaa cctagtaagg ccacctgcac gttctattaa agatgctaac ttttcgtctt
     4741 tagatgtcaa tacataagtg cccggataca atacttcacc tttcacggtt actttcattt
     4801 ggtattcaaa ttctggcgta gggcgaacat agatttggtc aaatggttgt aatttaacgg
     4861 agttggcaac aggatcttta cctatatttt tactaatact atagtttaaa attgttacct
     4921 gtgtaggtac agattgtgaa atggctttgt cataattagc aatacgtgca atttcaattc
     4981 tattagaagc agcctccatt ttaaagcctt gtgccaatat caaagcgtct gctaaggtca
     5041 tactttccat ataaggaaac gtatcatttt tacgcaccat accatttact tcgatataat
     5101 atttatctat tcttcttaaa tcttttaaca cataaatttt atcacgcttt ttcaatttaa
     5161 aagtatctaa agcctcgaaa ttattagttg tatcgattct aaaggagatt aatttttctg
     5221 tcaaatcatc atttactcga tagataataa ttttatctaa aagcgcttct tcttttaagc
     5281 cattacacat aattaatgca tcgcgaagtg acatgccttt ttctgattca aagcgtctag
     5341 gttttcttac ttcgcctttt acttcgatat caaatttgtc gaaataatct gtttttgcat
     5401 acacttcaac tacatctcta aaggtcagca acatattgtc ttctgaattt ttatcgtcga
     5461 taatattttg caaactcact ttataggtat gttttctaaa gtcgccataa tttgttctga
     5521 tcacgtatgc ttcgtctaag taagcatctt ctttcaagcc ttgcgcttta ctaattaaat
     5581 cccaaactct atcgccttcg cgccattcat atcttccagg caataacaca gaaccattaa
     5641 tatcattata gttttttact ttttctggaa tagagcgaac gccaacaaca tcaccatgct
     5701 ttaataaaat tttgccacca gacaaacgaa cgctatctaa attataatca attaatattt
     5761 gtttgttttc gtcgaatctt ctgatttgaa taacctttgt gaatgcatct ggtttatagc
     5821 caccacaata ttttaataaa tcttcaaatg tttcaccatt tttcatttca tatttccctt
     5881 ctctgtttat agcgccttca atacgaacaa tatttttttg tgctggcaca tagatataat
     5941 cattattatc taagaaaata tcatcttgtt tttctggcgt aaataagaat ttatataagt
     6001 cgaaatttct aatgattttt ccatcgcgtt ttatttgaat atcacgaata gaacctgatt
     6061 tagatggacc gccgacataa gctaaaatat tatatgcagt attgatagca gggaaagtat
     6121 aagagcctgg attttcaaca tcacctacta catttatcgt gattacacgt gaatagttaa
     6181 tagagacatc aaaatctgat ttatcaatca tatatgcatt ggcaaatcga gtacgcaata
     6241 agcctttagc ctgctctaaa gtcaaacctt tcagatacac tctaccgata ttttcttgtt
     6301 gtatgtatcc atctttatcg atataaaata agcgattgta atcggcataa ccccaaatag
     6361 aaacgactaa ttggtcgcca acacctaaga tataattgct aggtggtttt atatcgactg
     6421 cgttgttaaa tattttcaca tctttttgac ggaaataggt aaaaccataa atatcaaccg
     6481 tatccaattt ctcacgaagt tcgcgcgtag ctgttgcagt aattttttca actgtttcag
     6541 atgctttttg cgcttctttt aat
//
LOCUS       DAOQQV010000134         6541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5002_length_6541_cov_7.054887, whole genome shotgun sequence.
ACCESSION   DAOQQV010000134 DAOQQV010000000
VERSION     DAOQQV010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5002_length_6541_cov_7.054887"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLL59_11085"
     CDS             complement(<1..222)
                     /locus_tag="PLL59_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMY24395.1"
                     /translation="MKSLSIIRHAKSDWSTGVDDIARPLNERGKNAVVLIGNYLQQQK
                     IKPDLIISSPAKRAQDTAKAIARAIHYDVQ"
     gene            266..1750
                     /locus_tag="PLL59_11090"
     CDS             266..1750
                     /locus_tag="PLL59_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24396.1"
                     /translation="MNIKTELVELIDSLTPNEKAYISKYLTKTNKKDSVYLKLYRQII
                     KDKDIDLIKLNEQFKDSNISKNFSATFSYLYDQILNKLSNIEDKDINNTRIFYKINQI
                     QFLKNKALYNHCDKIIISLKSHLEKYEIYELQILVLQIEIELISYNKKAIDKKNELLQ
                     IRDKVVQCLQLENSAQLQVFHTEKLLFSHGVSSRKIKTITFKEILSILTEIQKKNTSL
                     KTKHYIAHALSNIAIVNNNYVEALNHLHELIQDYENKPTFKIYYIENYAKLLCNYINR
                     MVSADAYDRIFYLETILHEISTLTAKRKIEKTSVEDVYITYYHAMYAYNLKKENFDEA
                     FRMTQRMQDYKTPMERNKALFIIYHYDFACAAFYTQHYDLTLSYTNLLLQHENRNAFP
                     DVHIFAKLLQQFTWYMKEEYFLLQSQHEAIRKNIQNAPYDTTAEKAYLKMLFKLSNTS
                     DIDNKTTILVESESNIKSLIIKFPSALMFFNIFWWLKNEINRLK"
     gene            1901..2929
                     /locus_tag="PLL59_11095"
     CDS             1901..2929
                     /locus_tag="PLL59_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24397.1"
                     /translation="MKKLSFLLSLFFLSIITVYAQNIYTVNNNIGASANFNDLQVAVN
                     TVPANSILLVHGSAITYGNITVRKPLSIIGTGYFIGSNQAPYTQANLGTSKLYRLTLD
                     AGSNGSLVSGLTIDNWLVVDSTSNVTVSRNFINHGSYSTISYSTNVQFIQNYSNANLE
                     MHYSTVTITNNIFYNNSYLNHNYTTTTISNNIFDRNSNSYSVLNLGSGSSSNFSNNIL
                     INSATSASSTICGGCGAALTATNNVSSDSTFRGTNNAVLNAGITTIFSQYNNSNTSFD
                     SKYILTSGSPALGAGTGNTDAGIFAGINSYVLSGIPFIPNIYGLEIPNIGTTGSGLKV
                     RIKAQTNN"
     gene            3006..6323
                     /locus_tag="PLL59_11100"
     CDS             3006..6323
                     /locus_tag="PLL59_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY24398.1"
                     /translation="MLIASQSALAANITKLKYYIDDDTANITTHLLTPNASIDSLITL
                     TLVGISQGFHKLSVFVEDSDNKRSLTQEISFYYIHANSSQNEITKVEFIIDTLAPQTI
                     NIAPTSQLDELFTLNTTGIAPGMHILKVIVYGDDGTPSLTNYTTFFMYQGTGISNEIS
                     AFEVMIDNDTLNKQTISVSPSASFDNDISVNLSNVPQGNHQIKVTAIAQNGLRSLDNY
                     GSFMKVNGTTTDSIVALEYFFDTEPGIGNGTRINTARAHQIDSSISIMVPNNLSLGMH
                     RLYVRTQSNAGEWSLCQWDSLKICNIFPPTAGFNVARVGNTFSFLDTSNFATSWKWIF
                     GDGNTDTIQHPIHSFTPNNYIVKQNVKNTCSEDSIYSIIQVKGIESYFPQQSGLGNIY
                     IDFNGGGFDSSTIITIENNSQVYSPVKYFLSPFGNQISTHFDFHNATPGVYNIKIILE
                     DTTIFLANGFKILTQDETSRTLKIDLLGSTFVRATALNNNIIRITNLGNANAGVTGIG
                     LIIEDTTVEVFLKDSFIVFNASDSGYTVHIDSIGVEELRKSKGNTIDGRIYTIYIAGV
                     RAGGVYDIPIQIKYQNPGEKNLKIICKGPYNGSDFFGWFDDCDKARMQLVWDLTTDIL
                     GTVPVVNCAWAIAQASLGIFANLAAVPLKGSSYSGGDAVQTVYDMANTMLVKTPEIAI
                     QCSGEAGATLSAGATLVPEIIYGISKTIVDLYSNYQNMKEKCLDEEDDEDEKKIRVGT
                     SSDPNEIVGNNGYNTDRYINDKELMHYEVYFENVDTADLPAQIVTIYDTIDMNVLDIK
                     SFQFRGFGVGNTFYPASVDRKQYFEEFYHNGIRTRLNVNADTLTGIIAFNFVSLTAAG
                     FDLDDPLSGFLPPNNINNDGQGWVSYSIKQKSNLPHLTKIHNSASIIFDTNEPIHTNK
                     WTNTIDLQKPNSLVLSAHSIDDSLIVLKINRNDAHSGVKLYELGISINNRPYFTVPIR
                     IDSDSIILTGKMDSTYSFYSIALDNADNIEQKTPLAEASITLLPTSIKKNLLQHGIQI
                     FPNPTNGIIQVSFNKPLVGNLNYTITAIDGRIIQQRSLRQNANFSIDLNNENKGMYFI
                     QFKQDENILGTTKIIVQ"
BASE COUNT         2455 a         1056 c          993 g         2037 t
ORIGIN      
        1 ttgcacatca taatgtatag cacgagcgat agcttttgca gtgtcttgcg cacgctttgc
       61 aggcgaactg ataattaaat ctggttttat tttttgctgc tgtaaataat ttccaatcaa
      121 caccacagca tttttaccac gctcatttaa tggacgagca atatcatcaa caccagttga
      181 ccaatcggat tttgcatgcc gtataatgct taatgatttc attttattat tcttatttta
      241 taaatttaat cactattagc ttattatgaa tatcaaaaca gaattagtag aactaattga
      301 ttcgctaaca cctaacgaaa aagcttacat ttctaagtat ttaactaaaa cgaacaagaa
      361 agattctgta tatttaaaat tatacagaca gattattaaa gataaagata ttgatctcat
      421 aaaactaaat gaacaattta aggattccaa tatttcaaaa aatttttcag ctacattttc
      481 ttatttatat gatcaaattt taaataaact atcaaatata gaagacaagg atattaacaa
      541 tacacgtatt ttttacaaaa tcaaccagat ccaattctta aaaaacaaag cactatataa
      601 ccattgtgac aaaataatta tatcattaaa atcacatttg gaaaagtatg aaatttatga
      661 gcttcaaatc ctagttcttc agatagaaat agaattaatt tcctacaata aaaaagcaat
      721 tgataaaaag aatgaattgt tacaaataag agataaggtt gttcaatgcc tacaactaga
      781 aaattcagca caattacaag ttttccatac agaaaaacta ctattcagtc atggcgtgtc
      841 ttcaagaaaa ataaaaacaa taacttttaa agaaattcta tcaatattaa cggaaataca
      901 gaagaagaac acatctctca aaactaaaca ctacattgct catgcattga gtaatatagc
      961 tattgtaaac aataattatg tagaagcatt aaaccatctg cacgagttaa tacaagacta
     1021 tgaaaataaa ccaactttca aaatctatta catcgaaaat tacgctaaac tattgtgtaa
     1081 ttatatcaac agaatggtaa gcgctgatgc gtatgacaga attttctacc ttgaaacgat
     1141 actacatgaa atttcgactt taacagctaa aagaaaaata gaaaaaacga gcgtagaaga
     1201 tgtttacatt acttactacc atgctatgta tgcctacaac ctaaaaaagg agaactttga
     1261 cgaagccttt agaatgacac aacgcatgca agattataaa acaccaatgg aacgaaataa
     1321 agcattgttt attatctatc attacgattt tgcttgtgcg gctttttata ctcaacatta
     1381 tgatttaaca ctaagctata ccaacctatt attacaacac gaaaatagaa atgcatttcc
     1441 tgatgtacat atctttgcta aattattgca acagtttact tggtatatga aagaagaata
     1501 ttttctattg caaagtcagc atgaggcaat tcgcaaaaac atacaaaatg caccttatga
     1561 tacaacagca gaaaaggctt acttaaagat gttgtttaaa ctaagtaata cgagtgatat
     1621 tgataacaag acaactattt tagtagaaag tgaatctaat ataaaatcat taattataaa
     1681 atttccaagt gcactcatgt tctttaatat attttggtgg ctaaagaatg aaatcaatcg
     1741 tctgaaataa attatatttt aatataacaa tttggatatg agtgtgttaa atatacaaaa
     1801 agcacctcta aaacttaaaa acctacatag tttttcccaa catattataa aagtgattgt
     1861 taatttgcgt gaacattcaa taactataaa actttatact atgaaaaaat tatcatttct
     1921 tttatctctg ttttttttat caatcataac agtatatgca caaaatattt acactgtaaa
     1981 taataatatt ggtgcttctg caaatttcaa tgacttacaa gttgcggtaa acacagttcc
     2041 agccaactca attttattgg tacacggctc tgctattaca tatggaaaca ttacagtaag
     2101 aaaaccactt tctattattg gtactggtta ttttataggc agcaaccaag caccctatac
     2161 acaagccaat cttgggacgt ctaaattata taggctaaca ttagatgctg gctccaacgg
     2221 atctttagtg agtggtttaa caattgataa ttggcttgta gtggactcaa catcaaatgt
     2281 cacagttagt agaaatttca ttaaccacgg ttcttattca acaattagct attcaacgaa
     2341 cgtacaattc attcaaaact attcgaatgc taatttagaa atgcattatt caaccgttac
     2401 catcaccaac aatatctttt ataataactc ttatcttaac cataactaca ccaccactac
     2461 catcagcaat aatatatttg atagaaattc taattcatat tctgtcttaa acttaggcag
     2521 tggaagcagt tctaatttta gcaataatat tctcattaac agcgctacgt cagcatcttc
     2581 aacaatttgt ggtggctgtg gtgctgcatt aacagcaaca aataatgtat cctcagacag
     2641 tacgtttaga ggcaccaaca acgcagtatt aaatgcagga attaccacaa tattttcaca
     2701 atacaataac tcaaatacct cctttgattc gaaatatata ctaacttctg gttctccagc
     2761 attaggagca ggtacaggca atactgatgc aggcattttt gcaggtatta attcttatgt
     2821 tttatcagga ataccattta ttcctaatat ttatggttta gaaataccaa atattggaac
     2881 aacaggtagt ggattgaaag tgcgaattaa agcacaaacg aacaactaat aatcctattt
     2941 catcaccttc taaaaacaag agtatgaaac ggataatata tttcataagt tgcttgcttt
     3001 atataatgtt aatcgcatca cagagtgcat tagcagcaaa tattacaaaa ttaaaatatt
     3061 acatagatga cgatacggca aatatcacca ctcacttact cacgcctaat gcaagtattg
     3121 attcattaat tacattaaca cttgtaggca tttcacaagg ctttcataag ctttcagtgt
     3181 ttgtagaaga tagcgataat aaacgttcac tcacacaaga gatttcattt tactacatac
     3241 acgctaattc atcacaaaac gagattacta aggttgaatt tataattgat acacttgcgc
     3301 cacaaactat taatattgca cctacatctc aattagatga attatttaca ttaaacacaa
     3361 ctggaattgc accaggtatg catatattga aagtgattgt ttatggcgac gatggaacac
     3421 catcgcttac caattacacc acatttttta tgtatcaagg tacaggcatc tcaaatgaaa
     3481 taagtgcgtt tgaagtgatg atagacaacg acacactgaa taaacaaact atctctgtat
     3541 caccatctgc cagcttcgat aatgatattt ctgtaaacct atcaaatgtt ccacaaggca
     3601 accaccaaat aaaagttaca gcaattgcac aaaatggtct gcgctcatta gataattatg
     3661 gttcgtttat gaaagtaaac ggcacaacaa ctgacagtat tgtggcacta gagtatttct
     3721 ttgatacaga accaggcatt ggtaatggta cacgcatcaa tactgcacga gcacatcaga
     3781 ttgactcaag catatctatt atggttccaa acaatttgag tttaggtatg cataggctgt
     3841 atgtacgcac acaaagcaat gcaggcgaat ggagtttatg ccaatgggat agcttaaaga
     3901 tatgtaacat atttcctcca acagcaggct ttaatgtggc aagagttggc aatacattta
     3961 gttttctaga tacaagtaat ttcgctacaa gttggaaatg gatatttgga gatggaaaca
     4021 cagacacaat ccaacaccct atccattctt ttactcctaa taactatatc gtaaagcaga
     4081 atgtcaaaaa cacatgttcg gaagatagca tctatagtat tatacaagta aagggaatag
     4141 aatcttactt tccgcaacaa tctggattag ggaatatata tattgatttt aatggcggtg
     4201 gttttgactc aagtactatt attactattg aaaacaatag tcaagtatat agcccagtaa
     4261 aatattttct aagcccattt ggaaatcaaa tttcaactca ttttgatttc cacaatgcga
     4321 ctccaggagt ttataatata aagattattt tagaagacac cacgatattt cttgccaatg
     4381 gatttaagat actgactcaa gacgaaacct caagaacgtt gaaaatagac ttattgggca
     4441 gtacatttgt acgtgcgact gcattaaata ataacataat aagaatcaca aatcttggaa
     4501 atgctaatgc aggtgtaaca ggaattggac taataataga agatactaca gtagaagtat
     4561 ttttaaaaga ttcttttatc gtttttaatg caagcgattc tggatataca gttcatatag
     4621 atagcattgg agtagaagaa ctaagaaaaa gtaaaggaaa tacaatagat ggtagaatat
     4681 atacgattta tattgcagga gtaagagctg gtggagttta tgatatacct atccaaataa
     4741 aataccaaaa tccaggtgaa aaaaatttaa agattatttg taaaggacca tataatggat
     4801 cagatttttt tggttggttt gatgattgcg acaaagctag aatgcaactt gtttgggatc
     4861 ttactactga tattttaggt actgtaccag tagtaaattg tgcgtgggca atagcacagg
     4921 catcacttgg catatttgcg aatttggcag ctgtaccatt aaaaggttca tcatacagtg
     4981 gaggagatgc tgttcaaact gtttatgata tggcaaatac aatgctagta aaaacaccgg
     5041 aaattgcgat tcaatgttct ggtgaagctg gagctactct ttctgcaggt gccacattgg
     5101 taccagaaat catttatggt atatcaaaaa caatagtaga tctctattca aattatcaga
     5161 acatgaaaga aaagtgcttg gacgaagaag atgatgaaga tgaaaaaaag attagagtag
     5221 gtacttcttc tgatcctaat gaaattgtcg gaaacaatgg ttataataca gacaggtaca
     5281 ttaatgataa agaattgatg cactatgagg tatatttcga aaatgttgat acggcagact
     5341 tgcctgctca gatagttact atttatgata ctattgatat gaatgtatta gacattaaat
     5401 cattccaatt tagagggttt ggtgttggta atacttttta tccagcatca gtagatcgta
     5461 aacaatattt tgaagaattt tatcataatg gaattcgcac aaggctaaat gtaaatgctg
     5521 atactttaac tggtattatt gcttttaatt ttgtatcatt aactgctgca ggctttgatt
     5581 tagatgaccc attatctgga tttttaccac ctaataatat aaataatgac ggacaaggtt
     5641 gggtaagtta ttccattaaa caaaaaagta atttaccaca tttaactaaa atacataata
     5701 gtgcaagtat tatttttgat acaaacgagc caatacatac taataagtgg acaaacacta
     5761 ttgacctaca aaaaccaaat agcctagtat tgtctgccca cagtatagac gactcattaa
     5821 ttgttttaaa aataaatagg aacgatgcac attcaggtgt taagctttat gagttaggga
     5881 tttctattaa taatagacca tattttacag taccaataag aattgatagc gattctataa
     5941 tattaacagg aaaaatggat tctacgtatt ctttctattc tatagcatta gataatgcag
     6001 ataatataga acaaaaaacg ccacttgcag aagcgagtat tacattatta ccaactagta
     6061 taaagaaaaa ccttttgcaa catggcatcc aaattttccc caacccaaca aatggaataa
     6121 ttcaagtttc atttaataaa ccactcgtag gaaacttaaa ttatactata accgcaattg
     6181 atggaagaat aattcaacaa aggagtttac gtcaaaatgc aaacttttct attgacttga
     6241 ataatgaaaa taaaggtatg tactttatac aatttaaaca agatgaaaat attcttggaa
     6301 ccacaaaaat tattgttcaa taatttaaaa gatagaatgt aatgtttatt caagaggctg
     6361 tctcaaaggt cgagatagcc tcttttttat attaatttaa aagaatcttt atttgatatc
     6421 aaatagataa ttgtacaaaa aacccataaa aaattgcttt tttaaataaa tgtcgtatat
     6481 ttgcaccgaa aattgaatgg aagaggactt gacaaagtcc tcttttttat tcttatagca
     6541 a
//
LOCUS       DAOQQV010000135         6404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5170_length_6404_cov_6.992912, whole genome shotgun sequence.
ACCESSION   DAOQQV010000135 DAOQQV010000000
VERSION     DAOQQV010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5170_length_6404_cov_6.992912"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLL59_11105"
     CDS             complement(<1..865)
                     /locus_tag="PLL59_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMY24399.1"
                     /translation="MLFKDLSLIEPILRALQTEGYSTPTPIQAQSIPIILQQKDLLGC
                     AQTGTGKTAAFAIPMLQLLAEDFVKGKKTKSPKALILTPTRELAIQIDESFAAYGRHI
                     PLKRSVIFGGVSQHAQVMSLQQGVDIIVATPGRMLDLMSQGYVDLKAIQIFVLDEADR
                     MLDMGFVNDVKKIIAKLPQKRQTLLFSATMPKEIQDLANSILTHATKVEVTPVSSTAD
                     TVEQFIFFTEKKNKANLLTFLLKDKSIKSALVFTRTKHGADKVVKILNAATIKAAAIH
                     GNKSQNARQTAL"
     gene            1228..3123
                     /locus_tag="PLL59_11110"
     CDS             1228..3123
                     /locus_tag="PLL59_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HMY24400.1"
                     /translation="MSDPIKHECGIALIRLLKPLSYYKEKYGDLHYGLNKMYLLMEKQ
                     HNRGQDGAGLATIKLDTAPGKSYFDRERSVDKQAIKAIFDKVFQPFNTLADKEKDRLK
                     DMDYVKNNFPFMGELLLGHLRYGTHGGNDMSNCHPRIRANNWKTRTLIVAGNFNMTNV
                     DELFDRLVSLGQFPREMSDTMTVLEKIGHFLDANNQRLFKKFKTSGLSNADITPLIEQ
                     EINVADVLRNAAEDFDGGYAMAGMIGHGDAFVMRDPNGIRPAFFYKDDEVVVVASERP
                     AIQTAFNVHRSKVQEIKPGHALIIKKDGSVGEVECTKPLERKACSFERIYFSRGSDFE
                     IYQERKMLGKLLVPTILKAVNHDLQNTVFSYIPNTAEVAFLGMTEEVQKQHIELKLQA
                     FNRGEIKSTEELMTQLAIMPRVEKIAVKDVKMRTFITEDTSRNDLVHHVYDVTYGLVK
                     NNVDTLVVLDDSIVRGTTLKESILTMLDRLQPKKIIVVSSAPQIRYPDCYGIDMSKMG
                     SFVAFQAAVQLWKKKDDTFLPSLYKRCKEELEKPITEIENLVKEVYAPFSDEEISTEI
                     ANIVRPQHIFSDVQVIYQTIDNLHLACPNNQGDWYFTGNYPTAGGNRVTLNAFINYME
                     GKNVRAY"
     gene            complement(3231..4049)
                     /gene="panB"
                     /locus_tag="PLL59_11115"
     CDS             complement(3231..4049)
                     /gene="panB"
                     /locus_tag="PLL59_11115"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HMY24401.1"
                     /translation="MSSAQAEIKRVTTQKLREMKDRGEKISMLTAYDFSMAKILDDAG
                     IDILLVGDSASNVMAGHETTLPITLDQMIYHAQSVVRAIKRCFVVVDLPFGSYQGNSR
                     EALNAAIRVMKETGGHGVKLEGGQEVGESIIRILTAGIPVMGHLGLTPQSIYKFGTYT
                     VRAKEEEEAERLIEDAKYLESIGCFGLVLEKIPAALAKKVSESISIPVIGIGAGPHVD
                     GQVLVTHDMLGITHEFKPRFLRQYLHLYDDMKKATQQYIADIKSKDFPNEKEMY"
     gene            4230..5066
                     /locus_tag="PLL59_11120"
     CDS             4230..5066
                     /locus_tag="PLL59_11120"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HMY24402.1"
                     /translation="MDFLNNKSISELIAFGKQKNEEYVNAKPFPSAYFDDFFNPEMLR
                     EVLAEFPSIGKDKSDIKYENPNELKLATKGEYRFGEKTKAFVHFLNSQPFLEFLQNLT
                     GIKETLIPDPYFEGGGFHEIKPGGYLKMHVDFHKNKLTNLDRRLNILVYLNEDWQEEY
                     GGHFELWEKDMSKAVTKILPVFNRLAMFSTTDFSWHGHPDPLTCPPDRSRRSLALYYY
                     TNGRPAEEVKSGDGDKIVTTFAARKGQDSARMKFFNGFVNLVVECCPPILIKTIKKFR
                     NT"
     gene            5077..5823
                     /locus_tag="PLL59_11125"
     CDS             5077..5823
                     /locus_tag="PLL59_11125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HMY24403.1"
                     /translation="MKTINFLIFAFTRVQHIFYLFIAKLIGKEYQFITPAATYAPWVL
                     DKDFLATYTKVKKNSLLNFYQAWELWTLAANAASKEGNVLEVGVWRGSSSIMMGTKLQ
                     QLKSTKTIFACDTFEGVVKAGTSEDNYYKGGEHSDTSLQFVKDHVEQTFGLRNVQLLQ
                     GIFPDATGHLIENEKFCLCHIDVDAYISAKDIVTWLWPRMVIGGVIVFNDYGFPLTKG
                     ITLLVNEYLQREDAVRIHNLNGHGIIVKIK"
     gene            5830..>6404
                     /locus_tag="PLL59_11130"
     CDS             5830..>6404
                     /locus_tag="PLL59_11130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY24404.1"
                     /translation="MEKLQQCPCCLSEKIQAFDSLKDYHVTQEIFEIAQCHNCGFVFT
                     NPRPSESEIGRYYHSDLYVSHNSSATTLFHKLYRVVRDYMYNKKMKRIFSHLSNQNKN
                     FALLDYGAATGDFLAYCKTKGIVKISGIEQDDACRQKAMEQHAIALQPVNALQQLPTA
                     SYSVITLWHVLEHIHQLDDTLQSFHQLLNDDG"
BASE COUNT         2084 a         1081 c         1218 g         2021 t
ORIGIN      
        1 gcaaggcagt ttgacgtgca ttttgtgatt tatttccatg aatagcagca gctttaattg
       61 tcgcagcatt caatattttt acaactttat cagcgccatg cttggttcgt gtaaaaacta
      121 atgcgctttt gatggatttg tcttttaata aaaaagtcag caagttggct ttgttctttt
      181 tctcagtaaa aaagataaat tgttctacag tgtcagccgt tgatgataca ggtgttactt
      241 ctacttttgt cgcgtgtgtt aatatggaat tggccaaatc ctgaatttct ttcggcattg
      301 tggctgagaa taataaggtt tgacgttttt gtggcaattt tgcaataatc ttttttacat
      361 cattaacaaa acccatatcc aacattcggt ctgcttcgtc gagaacaaaa atttgtattg
      421 ctttcaaatc aacataacct tgactcatta agtctaacat tctacctgga gttgccacta
      481 ttatatcaac gccttgctgc aatgacatca cttgagcatg ttgcgataca ccaccaaaaa
      541 tgacacttct ttttaatgga atatgtcgtc cataagcagc aaaactttcg tcgatttgta
      601 tagcaagttc tcgtgtaggc gtgagaataa gtgcttttgg cgatttcgtt tttttccctt
      661 ttacaaaatc ttctgctaat aattgcaaca taggaatggc aaatgctgct gttttgccag
      721 tgcctgtttg cgcacagcct aataaatctt tttgttgtaa aataatagga atagattgtg
      781 cttgtattgg agttggcgtc gaataacctt cggtttgtag cgcacgtaaa attggctcta
      841 ttaatgataa atccttaaat aacattgttg tcgtaaatgg tatgtattga atatgcgtaa
      901 tacataaggt gtaaaataag cactttgctt gcccaaaaat gtaaatgatt tcttggccgg
      961 cacattcaag tgctcataaa aaatgcttag gctaactttg ggttgctagt aaatagcatt
     1021 aattaatcga tgtaaagata cgatgctaac attgtattct tatattatga tagcgttgtg
     1081 taaagccgta cctcaatcat gtactgttaa ttcttttgta tgtaataaaa ttaatttcca
     1141 caatcataat ccatatttca tatttactat cgccatcatt caaaaacaaa gcgtattttt
     1201 gcatatacca aattttccta ttaggaaatg agcgacccaa taaaacacga atgcggcata
     1261 gccttaatta ggctcttaaa accactgtct tactataaag aaaaatatgg cgacctgcac
     1321 tatggtctca ataagatgta tttgctgatg gaaaagcagc acaatagagg acaagatggt
     1381 gcaggattgg ctactatcaa attagatacg gcacccggaa aatcctattt cgatagagaa
     1441 cgtagcgtcg ataaacaagc tattaaagct attttcgata aggtttttca accttttaac
     1501 acacttgctg ataaagagaa ggatcgattg aaagacatgg attatgtgaa aaacaatttt
     1561 ccatttatgg gcgaattgct attgggtcac ttgcgttatg gaactcatgg cggcaatgat
     1621 atgagcaatt gccatccacg aattcgagca aataattgga aaacgagaac actcatcgtg
     1681 gcaggaaatt ttaatatgac caatgtagat gagttgtttg ataggttggt gagtttgggg
     1741 caatttcctc gtgaaatgtc agatacgatg actgttttgg aaaaaatagg tcatttttta
     1801 gatgccaata atcagcgttt atttaagaaa tttaagacct caggcttgtc caatgcagat
     1861 ataacgccgc ttattgagca agaaataaac gttgctgatg tgcttagaaa tgcggcagaa
     1921 gatttcgatg gtggttatgc aatggcaggc atgattggac atggagatgc gtttgtgatg
     1981 cgcgatccaa atggcattcg tcctgctttt ttttataaag acgatgaagt tgtagtcgtt
     2041 gcatcagaac gaccagcaat acaaacagca tttaatgtac atcgcagtaa agtacaggaa
     2101 ataaaacctg gtcatgcgtt gataataaag aaagatggca gcgttggcga agtagaatgt
     2161 acaaaaccat tggaaagaaa agcctgcagt tttgaacgca tctatttttc gagaggaagc
     2221 gattttgaga tttatcaaga gagaaaaatg cttggcaaat tattagtgcc tactatactt
     2281 aaagccgtaa atcacgactt gcaaaatact gtgttttcct atatacctaa caccgcagag
     2341 gtggcatttt taggcatgac agaagaagtg cagaaacaac atattgagct gaaacttcag
     2401 gcatttaatc gcggtgaaat caaatcgaca gaagaattaa tgacacaact agctatcatg
     2461 ccacgtgttg aaaaaattgc tgtcaaagat gtgaagatgc gcacctttat tacagaagat
     2521 acttcgcgta atgacttagt tcatcatgta tatgatgtta cctatggctt agtgaaaaat
     2581 aatgtcgata cattagtggt gttagatgat tctattgtta gaggcacgac actgaaagaa
     2641 agtattttga caatgcttga caggttgcaa cctaaaaaaa ttattgttgt ttcctctgca
     2701 ccacaaattc gatatccaga ttgctatggt atagatatga gtaaaatggg cagttttgtt
     2761 gcattccaag cggccgtgca actgtggaag aaaaaagatg atactttttt gccttcactc
     2821 tataaacgtt gcaaggaaga gctggagaag ccaatcactg aaattgagaa tttagtaaaa
     2881 gaagtgtatg caccattttc agatgaagaa atttctactg aaattgcaaa tatcgtaaga
     2941 ccacaacata ttttctcaga tgttcaggta atttatcaaa cgatagacaa tttacacctt
     3001 gcttgtccaa acaatcaagg cgactggtat tttactggaa attatccgac ggctggcggc
     3061 aatcgagtaa ctttaaatgc ctttattaat tacatggaag gtaagaatgt aagagcgtat
     3121 taagtaggtt tattagtaat attttttatc tgaaaatgac aaagttgtct ttgacgttct
     3181 gtaggaaaga aatattatta tgtaaggcaa cacaacagcc tgtctgaatc ctaatacatt
     3241 tccttttcat tcggaaaatc tttgctttta atatcggcaa tgtattgttg tgttgccttt
     3301 ttcatatcat cgtataaatg cagatactgt cttagaaaac gaggtttaaa ctcatgtgta
     3361 atgcctaaca tatcatgcgt tactaatact tgaccatcga catgcgggcc agcgccaatg
     3421 ccgataactg gtatagaaat actttctgaa actttttttg ctaatgccgc agggattttt
     3481 tctaacacca atccaaagca accaatactt tctaaatatt tagcatcttc aattaatcgt
     3541 tctgcttctt cttcttcttt tgctcttact gtgtaagttc caaatttata tatcgattgt
     3601 ggcgttaaac ctaagtgtcc cattacagga attccagctg tcaaaattct gatgatggac
     3661 tctccaactt cttgtccacc ttctagcttt acgccatgtc cgccagtttc cttcatcacg
     3721 cgtatggctg cgtttaatgc ctcacgagaa ttaccttgat aagaaccaaa cggcaaatct
     3781 accactacaa aacagcgttt tatggcacgt accacacttt gagcatgata aatcatttgg
     3841 tctaaagtga tgggcaaggt tgtttcatgg ccagccatca catttgatgc agagtcgcca
     3901 actaataaga tatcaattcc agcatcgtct aaaattttcg ccatggaaaa atcatatgcg
     3961 gtaagcatgg atattttttc accacgatct ttcatctcgc gtagtttttg tgtagtaacg
     4021 cgttttattt ctgcttgtgc actagacata tttataattt aaaaagtgaa ggtatgctaa
     4081 tttaattgga atgatgttaa tataaaatta gcattttttg tcataattac gaatatgtcg
     4141 taggtaagta ggttttatgt acatttgtat tgtggcgaaa tatcgataag aggttcggca
     4201 atattacgtt acacaaaaaa taacaatcaa tggatttttt aaacaataaa tcaatttcag
     4261 aattaatagc gtttggtaaa caaaaaaatg aggaatacgt aaatgctaaa ccatttccga
     4321 gtgcttactt cgacgatttt tttaatcctg aaatgcttcg ggaagtgttg gctgaattcc
     4381 catcaatagg gaaagataaa tcagatatca aatacgaaaa tccgaatgaa ttaaaattag
     4441 cgacaaaagg agaatatcgt tttggtgaaa aaacaaaagc atttgttcat tttctaaact
     4501 cacaaccttt tctggaattt ctacaaaatt tgacaggcat aaaagaaaca ttaattccag
     4561 atccttattt tgaaggtgga ggcttccatg aaataaaacc tggtggctac ttaaaaatgc
     4621 atgttgattt tcataaaaat aaattgacaa atttagatag acgactaaat attttagtgt
     4681 atttgaatga agactggcaa gaagaatatg gtggtcattt tgaactttgg gaaaaagata
     4741 tgagtaaagc agttacaaaa attttgcctg tttttaatcg cttggccatg ttctctacaa
     4801 cagacttctc ttggcatggc caccctgatc cgctgacttg tccgcctgac agaagcagac
     4861 gctcattggc tttatattat tacaccaatg gaagacctgc agaagaagta aaaagtggcg
     4921 atggcgataa gatagtcacg acatttgctg cgcgtaaagg acaagattcg gcacgcatga
     4981 aattctttaa cgggtttgtt aatttagttg ttgaatgctg cccgccgata ttaatcaaaa
     5041 cgataaaaaa atttagaaat acctaattcc tacttgatga aaacaatcaa ctttttaatt
     5101 tttgcattca cccgtgttca acatattttc tatttattta ttgccaaatt aattggcaag
     5161 gaatatcagt ttatcacacc tgccgcaaca tacgcgcctt gggttttgga taaagatttt
     5221 ttagccactt atacaaaagt aaaaaagaac tcactcttga atttttatca agcttgggaa
     5281 ctttggactt tagcagcaaa tgccgcatct aaagaaggta atgtattgga agtaggcgtg
     5341 tggcgtggtt ccagctctat tatgatgggc acaaaattgc aacaattaaa aagcacaaaa
     5401 actatttttg cttgcgatac ttttgaagga gtagtaaaag caggtacttc agaagataat
     5461 tattacaaag gtggtgaaca tagcgacacc tctttacaat ttgtaaaaga ccatgtagag
     5521 caaacatttg gtttgcgaaa tgtacaattg ttacaaggca tttttccaga tgcaactggc
     5581 catctgattg aaaatgaaaa attttgtttg tgccatatcg atgttgacgc atacatttct
     5641 gcaaaagaca tcgtaacatg gctgtggcca cgaatggtga ttggaggcgt tattgttttt
     5701 aacgattatg gatttccatt aaccaaaggc attactttat tggtgaatga atatttgcaa
     5761 cgcgaagatg ctgttcgaat ccataacttg aatggtcatg gtattattgt aaaaattaaa
     5821 taagcacaat tggaaaaatt acagcaatgt ccgtgttgtt tgagcgaaaa aatacaagca
     5881 tttgattcgc taaaagacta tcatgttaca caagagattt ttgaaatagc gcaatgtcat
     5941 aattgtggtt tcgtatttac aaatccacgc ccaagcgaat cagaaattgg cagatattat
     6001 cattcggatt tgtatgtttc acataattca tcagctacaa cactttttca taagttgtat
     6061 agagtcgtga gagattatat gtataacaaa aaaatgaagc gtattttttc tcatttatcg
     6121 aatcaaaata aaaattttgc attgctggat tatggtgcag caacaggcga ttttttagca
     6181 tattgcaaaa cgaaaggaat tgtaaaaata agcggcatag aacaagatga tgcctgccgt
     6241 caaaaagcaa tggagcaaca tgcaattgca ttacaaccag taaacgcact tcaacaatta
     6301 cctactgcat cttattctgt gattacctta tggcatgttt tagagcatat acatcagtta
     6361 gatgatacat tgcagtcatt tcatcaattg ctaaacgatg atgg
//
LOCUS       DAOQQV010000136         6382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5200_length_6382_cov_9.044097, whole genome shotgun sequence.
ACCESSION   DAOQQV010000136 DAOQQV010000000
VERSION     DAOQQV010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5200_length_6382_cov_9.044097"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1439
                     /locus_tag="PLL59_11135"
     CDS             162..1439
                     /locus_tag="PLL59_11135"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HMY24405.1"
                     /translation="MKEKLLHFIWQNKLFNTKTLITAEHNELQILDFGRYNTDGGPDF
                     CNAKIKIDDVVLVGNIELHVYASDWKLHKHEHDKKYNNVILHVVYFHDYDIPNINTLV
                     LNGRISIRLLERYQSLMQSKQEIVCRAHINEVDTFTIENWKERLLIERFEYKSAYILQ
                     QLKHQQNDWEKTCYHLIGKYFGSHINNDAFELLCNYLDYKILLKHQDNIFQLEALLYG
                     VAGFLNKDFIEIYPRKLKQEYQFLKHKYGLQQMQEHHWQYLRIRPISFPTIRIAWFAS
                     LMQSMPLMQNLVHTQTSRQALDNIDVSEYWNSHYMFDKISKIKSKKMGNEFKAILHIN
                     VIAPLLYAYAKYSNDEKYRTAAFEILYTTKPETNAKIKFFDGMNLQQQHAFDTQALIA
                     LYDQYCSNKRCLKCGIGNKILRNNQTTNAINFA"
     gene            1449..1697
                     /locus_tag="PLL59_11140"
     CDS             1449..1697
                     /locus_tag="PLL59_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HMY24406.1"
                     /translation="MLEKPFKIFQSRTLRDVIEINVYGVCAYLGRKLSMPSKRVRLFF
                     IYTSFIALGSPIIIYLILTFVLNLRYMINNKRNPVWDI"
     gene            1731..2213
                     /locus_tag="PLL59_11145"
     CDS             1731..2213
                     /locus_tag="PLL59_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HMY24407.1"
                     /translation="MKKSAPTINLSAEQIIQKLEIFCAYRERCSSEIIQKMVQLKVEQ
                     NDYNTYIDYLRDNNFFNEERYVASFVRGKATIKNWGKRKITQALQQKKIDSKLIQENI
                     ENLDEGVYFMQLNDLILKKLATLKAKDNFEKKQKLIRFAMQKGYELRWINEVLNEINL
                     "
     gene            2216..2602
                     /locus_tag="PLL59_11150"
     CDS             2216..2602
                     /locus_tag="PLL59_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMY24408.1"
                     /translation="MNRITYTEGSPWEPIIGYARAVKTGNIIEISGTTATVNGEVVGK
                     GDVYEQTKCILGKFDKVLQELGASMNDVVRTRIFCTDISQWEAIGKAHGEFFSEIKPA
                     TGMYQISKLISDDLLIEIEATAIINQ"
     gene            2642..2860
                     /locus_tag="PLL59_11155"
     CDS             2642..2860
                     /locus_tag="PLL59_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24409.1"
                     /translation="MKTKKISYLLLLFVCVATMVSCREGVGCPADQYNDWKKIEAARK
                     QQLKNPNNPNKGKNKKGPNVVGNGVVPK"
     gene            2841..3665
                     /locus_tag="PLL59_11160"
     CDS             2841..3665
                     /locus_tag="PLL59_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24410.1"
                     /translation="MEWCRNKLLCNIAIISLMFAIACTKNTTPPRIDTRQLPSKVIRY
                     EYDTLIGAENPPCCEIKIDYNFELNRYDSIRIGDTRKFDFDYSKFANEGILLATYSDI
                     HGAYDRIHFNTQKNIIEKLVSNGQTDSTIMSFDSLGRILSLQYDDAFSPLNSSIDYVY
                     KYDTVFRTKKYNSGTCEQVDTLLTIYKKFNNQLPYLMLSDFYSPCNIRLRTSLLHALP
                     TSSYNYAIPRTVLSGENMTSFTYTADAKGRLASLSISTINRLSLRIVEQRRYVLAY"
     gene            complement(3683..5842)
                     /gene="recG"
                     /locus_tag="PLL59_11165"
     CDS             complement(3683..5842)
                     /gene="recG"
                     /locus_tag="PLL59_11165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HMY24411.1"
                     /translation="MIFAFEFMLFHTFAIVQVSNNSFLSTPIEFLKGVGPERAAVLKK
                     EASIFTFGDLLTLFPYRYIDRTKFYSTQHIDNTQTYIQLKGKLKHIREIGEGRGKRLT
                     AIFYDNDGEIELVWFQGVKWLVNSLDTRETYILYGKPSWFNGKFNITHPELEKLATFN
                     DSDVGTFRALYNSSEKMKAKSLDSKGISKLMLTLLQQLRQEHIVENLPKHLLDKYKLF
                     GKFDAIKNIHLPQTLSLQAEAIKRLKFEELFLIQLQLLQLKIIRKREKGIYFEKVDHY
                     FKKFYDDALPFSLTNAQKRVLKEIRKDTLSQQQMNRLLQGDVGSGKTIVALLSMLMAI
                     DNNYQAAMMAPTEILAQQHYVGIKELCDKIGIRIALLTGSTKSKERKQLLHSLQHGEI
                     HLIIGTHALLEDSVQFNNLGLVVIDEQHRFGVAQRAKLWKKNNIAPHILVMTATPIPR
                     TLAMTLYGDLDVSILDELPPGRKPIKTVHRYESQRLAVFGFIRDEIAKGRQVYIVYPL
                     IEESEKSDLVDLMNGFESISRAFPLPDYCVSIVHGKMKPENKDFEMQRFIRGETQIMV
                     ATTVIEVGVNVPNASVMIIENAERFGLSQLHQLRGRVGRGAEQSYCILMTTHKLTADG
                     KKRINTMVETNDGFKIAEVDLELRGPGNMEGTQQSGNIDLKIANIASDGHIIEEARKA
                     AIALLEDDATLQKQENIVLKQYLQQQKIDNGFWRNIS"
     gene            5927..>6382
                     /locus_tag="PLL59_11170"
     CDS             5927..>6382
                     /locus_tag="PLL59_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HMY24412.1"
                     /translation="MEKKINAFCDDALGKMDATALAAAIASKKVSVDEVTAAAIARAE
                     KVNPSLGAIVLRTYDDAKNTARLKTNGIFYGVPTFVKDNDFIKGLPTQKGAIAYKSKI
                     AKSNSKYVNQFFSTGVNCLGKSTLPEFGFICSTENENLQVTRNPWNINYT"
BASE COUNT         2195 a         1099 c         1062 g         2026 t
ORIGIN      
        1 gcttcgacat gcaagtagac ccttgtaaag tttgtccgtt ctaaaatgaa tagatttatt
       61 ctttatataa aaatagcctt cgattaaaga aggctatttt ttttactata aatttatgga
      121 aaatagccat aatcggtctc tcaatagata aaccgattat gatgaaagaa aagttattgc
      181 attttatttg gcagaataaa ttattcaata ccaaaacact gattacggca gagcataatg
      241 agttacaaat cttagatttt ggcagatata atactgatgg cggtccagat ttttgtaatg
      301 ctaaaataaa aattgatgat gtggttcttg taggaaatat tgaactacac gtatatgcat
      361 cagattggaa attacataag cacgaacatg ataaaaaata caacaatgtg atattgcatg
      421 tcgtatattt tcatgattac gatataccta atataaatac attagtacta aatggaagga
      481 tttcaattcg attgttagaa cgatatcaat cgcttatgca atctaaacaa gaaattgtgt
      541 gtcgagcaca tatcaatgaa gtggatactt ttactataga aaattggaaa gaacgattgc
      601 ttatcgaacg ttttgaatac aaatcagcat atattctaca acaattaaaa catcagcaaa
      661 acgactggga aaaaacttgc tatcacctta taggtaagta tttcggcagc catatcaata
      721 atgatgcatt tgagttgttg tgcaattatt tagattataa aatattactc aaacatcaag
      781 ataacatctt tcaactggaa gcattattgt acggcgttgc cggattttta aataaagatt
      841 tcattgaaat atatccaaga aaattaaaac aggaatatca atttttaaaa cataaatatg
      901 gcttacagca aatgcaagaa catcattggc agtacttacg tattcgtccg atttctttcc
      961 caacgatacg tattgcatgg tttgcaagct taatgcaatc gatgccactc atgcaaaatc
     1021 tggtgcatac acagaccagt aggcaagcct tagataatat tgatgtttct gagtattgga
     1081 attcacatta tatgtttgat aaaattagca agataaaatc caaaaaaatg ggcaatgaat
     1141 ttaaagcaat tttacatatc aatgtaatag ctcctctttt atatgcctac gctaaataca
     1201 gcaacgacga aaagtataga actgctgcct ttgaaatttt gtacacaaca aaacctgaaa
     1261 caaatgcaaa aataaaattc tttgatggta tgaatttaca gcaacaacat gcatttgaca
     1321 cacaagctct gatagcgctt tatgatcaat attgctctaa taaacgatgt ttgaagtgtg
     1381 gtattggtaa taaaatttta cgaaacaatc agacaacaaa tgcgattaat tttgcataac
     1441 tttattgtgt gttagaaaag ccttttaaaa tattccaatc tcggacactg cgtgatgtca
     1501 tcgaaatcaa tgtttatggc gtctgtgcat atttaggacg taaattatct atgccatcta
     1561 aacgtgttcg tctatttttt atctacactt ctttcattgc attaggctca ccaattatta
     1621 tttatctgat tcttactttc gtgcttaatt tacgctatat gataaataat aaacgaaatc
     1681 ctgtttggga tatttaataa atagcattaa tgccattgtt gtaaaatcga atgaaaaaat
     1741 cagcaccaac tattaatcta tcagcggaac agattattca gaaattagaa attttttgtg
     1801 cttacagaga acgctgctct tccgagataa tacaaaaaat ggtgcagcta aaggtcgaac
     1861 aaaatgatta taatacttat atagattatc tcagagataa taattttttt aatgaagagc
     1921 gttatgttgc atcattcgtt cgtggtaagg caacaatcaa aaactgggga aaaagaaaga
     1981 ttacacaagc attgcaacaa aaaaaaattg actctaaatt aatacaagaa aatatcgaaa
     2041 atttagatga aggtgtttat tttatgcaat tgaatgattt gatattaaaa aaattagcaa
     2101 cgttaaaagc aaaagataat ttcgaaaaaa aacaaaaatt aattcgcttt gcaatgcaaa
     2161 aaggatatga actacgctgg ataaatgaag tattaaatga aataaattta taaatatgaa
     2221 cagaataaca tacacagaag gctcaccatg ggaaccaata atcggctatg caagagcagt
     2281 aaaaacaggt aatatcatcg aaatatcagg cacaacggca acggtaaatg gcgaagtagt
     2341 tggaaaaggc gatgtgtatg aacaaacgaa atgtatttta ggaaaattcg ataaagtgtt
     2401 acaagagctt ggcgcaagca tgaacgatgt agtgcgcaca cgtattttct gcacagacat
     2461 ttcacaatgg gaagccatcg gaaaggcaca tggcgagttt tttagcgaaa taaaacctgc
     2521 aacaggtatg tatcaaattt cgaaattaat ttctgatgat ttattaatag aaattgaggc
     2581 aactgcgata ataaaccaat aaacacttgc agtttattag aattttagta cctttatttt
     2641 tatgaaaaca aaaaaaatat cctacctttt attgttgttt gtttgtgttg ccacaatggt
     2701 ctcttgtcgt gaaggcgttg gctgtccagc tgaccaatat aacgattgga aaaaaattga
     2761 agcagccaga aaacaacaat taaaaaaccc aaacaatccg aataaaggga aaaataaaaa
     2821 aggaccaaac gtagtaggta atggagtggt gccgaaataa actgctgtgc aatattgcca
     2881 tcatatcttt gatgtttgcc attgcctgca caaaaaacac cacgccacct agaatagaca
     2941 ctcgtcaatt acctagcaaa gttattcgct acgaatatga tacattaatt ggtgcagaaa
     3001 atccgccatg ctgtgaaatt aaaatagact acaattttga actaaataga tacgatagca
     3061 ttcgcattgg cgatacaaga aaattcgatt ttgattattc caaatttgct aatgaaggca
     3121 ttttgctagc cacttactct gatatacatg gtgcatatga tagaattcat ttcaatactc
     3181 agaaaaatat tattgaaaaa ttagtgagta atggccagac agattctact ataatgtcgt
     3241 ttgattcttt aggtagaatt ttgtctttac agtatgatga tgcattttca ccgctaaatt
     3301 cttcgataga ttatgtatat aaatacgaca ctgtttttcg aacaaagaag tacaactccg
     3361 gcacttgtga acaagtagat acgctattaa ccatctataa aaaattcaat aatcaactgc
     3421 catatttgat gttgagcgat ttttattcac cttgtaatat aagattaaga acaagtttgt
     3481 tacacgcatt gccaacttcc agctataact atgccattcc tcgaacagtg ttaagtggcg
     3541 aaaatatgac aagctttaca tatacagcag atgcaaaagg taggttggct tccttaagta
     3601 taagtactat taataggctt agtttgcgta ttgtagaaca aaggcgatat gtgcttgcgt
     3661 attgaccttg tttgaatatt catcagctaa tatttcgcca aaatccattg tcaatttttt
     3721 gttgttgcaa gtattgtttt agaacaatat tttcttgctt ttgcaatgtt gcatcatctt
     3781 ctaataaagc aatggcagcc tttctagctt cttctatgat atgtccatcg cttgcgatat
     3841 tggctatctt taaatcgata ttgccacttt gttgagtgcc ttccatattg ccagggccac
     3901 gtaattctaa atccacttcc gcaatcttaa atccatcatt ggtttctacc atggtgttga
     3961 tacgcttctt gccgtcagca gtcaatttat gtgtcgtcat taaaatacaa tacgattgct
     4021 ccgcaccacg accaacacga cctcgcaatt gatgtaattg cgacaatcca aaacgctctg
     4081 cattttctat aatcatcaca ctagcattgg gcacatttac gcctacttca atcaccgtcg
     4141 tggcgaccat tatttgtgtt tcacctctga tgaatcgttg catttcaaaa tccttattct
     4201 ctggcttcat tttaccatgc acgatactca cacaataatc tggtaatgga aaagcacggg
     4261 aaatgctttc aaaaccattc atcaaatcta ccaaatcgct tttttcgctt tcttcaatta
     4321 atggataaac gatataaacc tgacgtcctt ttgcaatttc atctcgaata aaaccaaata
     4381 cagcaagtcg ctgcgactca tagcgatgca ccgtttttat tggctttctg ccaggcggta
     4441 attcgtctaa aatagacaca tctaaatcgc cgtataatgt cattgccaat gtgcgtggta
     4501 ttggtgtcgc cgtcatcact aaaatgtgtg gtgcaatgtt gttctttttc caaagcttcg
     4561 cacgttgtgc aacaccaaaa cgatgttgct catcaataac aactaaacct agattattaa
     4621 actgaacact atcttctaac aatgcatgtg tgcctattat aagatgtatt tcgccatgtt
     4681 gcaaactgtg taataattgc ttgcgctctt ttgatttagt tgagcctgtg agcaaagcta
     4741 tgcgaatgcc tattttatca caaagctctt ttatgccaac ataatgctgc tgtgccaata
     4801 tttctgttgg tgccatcatg gctgcttggt aattattgtc gattgccatt aacatagaca
     4861 ataaagcaac aatggtcttt ccagagccaa catcaccttg caaaagccta ttcatttgct
     4921 gttgcgataa agtatctttt cttatttctt tcaatacacg cttttgcgca ttggttaatg
     4981 aaaagggcaa tgcatcgtca taaaactttt taaaataatg atctaccttt tcgaaataaa
     5041 ttcccttttc tcgttttctt atgattttta attgcaataa ttgcaattgt atcaaaaata
     5101 attcttcaaa ttttagtcgc tttatagcct cagcttgtaa acttaatgtt tgcggtaaat
     5161 gtatgttttt tatagcatca aattttccaa atagtttata cttatctagc aaatgctttg
     5221 gcaaattttc tacaatatgt tcctgcctta attgttgcaa taaagtaagc attagcttag
     5281 agatgccttt gctgtctaaa ctttttgcct tcattttttc agatgaatta tataatgcac
     5341 ggaaagtacc aacatcactg tcgttaaagg tggctagctt ttctagctct ggatgtgtga
     5401 tattgaattt gccgttgaac cagctcggtt ttccatataa aatataagtt tctctcgtgt
     5461 ctaagctatt caccagccat tttacacctt gaaaccagac taattcaatc tcgccatcat
     5521 tgtcataaaa aatagcagtt aaacgcttgc ctctgccttc gccaatctcg cgtatatgct
     5581 tcaattttcc ttttaactga atatatgttt gcgtgttgtc aatgtgttgt gtggaataaa
     5641 atttagttcg gtcaatatat cgataaggaa atagtgtaag caaatcgcca aatgtgaaaa
     5701 tcgaagcttc cttttttaaa acagctgcac gctcaggacc aactcctttt agaaattcaa
     5761 taggcgtaga taaaaaacta ttgtttgaca cttgcacaat agcaaaggta tgaaaaagca
     5821 taaattcaaa tgcgaatatc attaatgaat tttcatctta tgaatgtgtg ttatcaattt
     5881 gacaaaaagc aacttgagtt tgtaattagt ttagtaattt taaaaaatgg aaaaaaagat
     5941 aaacgcattt tgcgatgacg cattgggcaa aatggatgca acagcattgg cagcagcaat
     6001 tgcaagtaaa aaagtatcag tcgatgaggt aacagcggca gccattgctc gcgcagaaaa
     6061 agtgaatccg agcttaggcg ccatcgtgct tagaacttac gatgatgcta aaaatacagc
     6121 acgattaaaa acaaacggca ttttttatgg cgtgcccaca tttgtaaaag acaatgattt
     6181 tattaaagga ctaccaacgc aaaaaggtgc aattgcatac aaatcaaaaa ttgctaaatc
     6241 taatagcaaa tatgtcaatc aatttttttc tactggcgta aattgtttag gcaaatctac
     6301 actaccagaa tttggattta tttgtagcac agaaaatgaa aacttacagg ttactcgtaa
     6361 tccttggaat ataaattata ca
//
LOCUS       DAOQQV010000137         6231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5357_length_6231_cov_7.166289, whole genome shotgun sequence.
ACCESSION   DAOQQV010000137 DAOQQV010000000
VERSION     DAOQQV010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5357_length_6231_cov_7.166289"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLL59_11175"
     CDS             <1..684
                     /locus_tag="PLL59_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24413.1"
                     /translation="SVYESGMYLTVKQKTSNLGNFFILLKDKKKLIRYISIILLAIPI
                     WYVVQLYAKYAPELADALGLQYDDKKKVAIYAIMIIYLGLTIGDVSCGLISNYLKSRK
                     KAILLYICVLSVFIIVFWWIGKINMTFFYTMIFLLGIGIGYWAVFMSTAAESFGINIR
                     STVSNTAPNFVRGAVIGINYLYITFKTNFHFSTQMANLSVGVICIGIAIIAWTQIEET
                     YGKDLDYVE"
     gene            754..2385
                     /locus_tag="PLL59_11180"
     CDS             754..2385
                     /locus_tag="PLL59_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY24414.1"
                     /translation="MKTKLTNLLLFAVVFVSFTNVYAQKVKKNSSVDVSADTPGSTMD
                     AKTAKKYYNVDPKKYLKDWNLTVHFGTTTPFTDIRSYDWSRQLKKPSELQWGAGLGLT
                     KMFNSAFGLNVDYTLGKLLGRTVERGGFAEERQYWKQLFPERTEADGPVYFKTNVFHQ
                     ASVNFYIDWLGLGTAYNKFIKSQITGKPIKSRKFAFYTKLGIGIIRASSQIYNSKDDK
                     PIANNAYLRGYTNKFTEVVFPMAFGMKFKVSKSFDIGLEGNFTFTNSDKLDALQFQSR
                     VDAGGKTVNSLSKINRDAYAYVNVNFSYKFGRIGSQKEHVEWVNPIAFIMANVPKPKE
                     VNLKDTDGDGVLDILDKEPTTEPGLAVNVHGVTLDSDKDGCPDSKDPEPFSSPAFPIV
                     ECKNVLPEAPAVTEQVVKGGVPYDDEAVNKKIQKVEDNIWKLTSVYFDVNKYNITSAG
                     SEELKKIGIILSTNPNLKVNVKGNTDVRGSTDFNQKLSENRVNAAIKYLKDHYNIADD
                     RFVKLPMGKSDPLVKDAVNENQHQSNRRVDFSPVR"
     gene            2447..3442
                     /locus_tag="PLL59_11185"
     CDS             2447..3442
                     /locus_tag="PLL59_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMY24415.1"
                     /translation="MKNKIFKSNPDLQFIRNDIKGNLFIDGKYVLKEEKDKQPLGKFL
                     KWISSKNPQKTEKKQDTFIPELIKNDTFTHSSIDKIVWLGHSSFYIQINKVRILTDPV
                     FYSLTPLLKRKHKLPCTIDEIKNIDYILLSHGHRDHLDVSTLRKILLYNPTVEILCPL
                     GFKKMLASIGFKKIQEAAWWQQYIVNKLDITFLPAKHWNRRFITDYNKTLWGSFAIKS
                     KNTSLYFAGDTGFDAHFEEIHKYFSSFDFCLMPVGAYKPRYIMKWAHVSPEEAVQAFN
                     ILHGKIFIPMHYGTFDLSDEPASEPVKILKHLERENEINGDIEILAVGEEFYIKH"
     gene            complement(3439..3717)
                     /locus_tag="PLL59_11190"
     CDS             complement(3439..3717)
                     /locus_tag="PLL59_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMY24416.1"
                     /translation="MSYEVKVILDGNKYDIKVAEGETILEAAINANIDPPYACRVAAC
                     CSCKAKLIDGKVEMDDDEPLTEEELDEGFILTCQSHPKTNCTINYDEA"
     gene            3953..5698
                     /locus_tag="PLL59_11195"
     CDS             3953..5698
                     /locus_tag="PLL59_11195"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMY24417.1"
                     /translation="MQTTIIRNGIYFDGLGNKGIKKDVIIKDNKIEAVVDKAPNIENA
                     KEIDATDKWIMPGLLDIHAHYDAELEVLPALEESVRHGVTTVIIGNCSLSAALGKEEE
                     IVDLFARVENIPAKVLSQWMIGNIKWKNVREYYEHLDTVPVGPNVASFLGHSNLRMHV
                     MGLDRSFTQKNASEDELKKMEAIVEEALDAGYLGVSIDMLPFHRWAGVYAPKYTGFSV
                     PSQLAAKSEYKRLANVLRKRNRILQITPNAIEKTSFMTITRLSQGGFFKKSLKTTIVA
                     ALDLKSNEKIYNLLKLLGFIGNKILRADLKFQTLSVPFLNYGDGPVSPLFEEFPSMIK
                     AISADAAGRHAMFTDPTFRKQFVKEWNHKAASVFPKALKEMWIVNSPVSTHIGKNFEQ
                     VAKEAGVEPVEHFMNLLAEYDTKIRWKCETSNHRENVRMELLNSEHCFPGFNDSGAHN
                     LNMAFHDGGLHLFKQAMRHPETMPIERAVYRLTKEPADYLGIDVGDISVGKRADLIII
                     DPTRLESDLTTEPIEDYHPQFEGSYRLIKRSGKVVQHVFINGNEAYTNNGEVKFHEDL
                     GKKKIFGRLLRSNWN"
     gene            complement(5807..6013)
                     /locus_tag="PLL59_11200"
     CDS             complement(5807..6013)
                     /locus_tag="PLL59_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24418.1"
                     /translation="MDKSKMIKAYCLKTKEKDVPMHDAVITKTSRGGYMASGNDGKGN
                     KMAAILGEAKALQAIKDGVAKQGF"
BASE COUNT         2210 a         1003 c         1032 g         1986 t
ORIGIN      
        1 agtgtatatg agtcaggaat gtatttgacc gtaaaacaga aaacctcaaa tcttggaaac
       61 ttttttatcc tattaaaaga caagaaaaaa ttaattcgct atatatctat tattttatta
      121 gctattccaa tttggtatgt agtgcaatta tatgctaaat acgcaccaga attagcagat
      181 gcattaggac tgcaatacga tgataaaaag aaagttgcca tttatgccat tatgataatt
      241 tatttaggat taactattgg tgacgtcagc tgcggcttga tttctaatta tctaaaaagt
      301 cgcaaaaaag caatactatt atatatatgt gtattgagtg ttttcatcat cgtattttgg
      361 tggataggaa aaataaatat gacatttttt tacactatga tttttctact tggaattggc
      421 atcggttatt gggcagtatt tatgagtacc gcagcagaat cttttggtat aaacattcgc
      481 tctacagtga gcaacacagc accaaatttt gttagaggtg ctgtcatcgg aatcaattat
      541 ttatatatta cattcaagac aaacttccat ttttccacgc aaatggctaa tttatcggtt
      601 ggtgtaattt gcattggaat cgccatcata gcttggacac aaatagaaga aacttatggc
      661 aaggatttag actatgttga ataattatta attattgtct gatttttttg tatattaaca
      721 aacattcact atttttactc aaaatctaac ttaatgaaaa ccaaactgac caacttgcta
      781 ctttttgctg tcgtttttgt ttcttttaca aatgtttatg ctcaaaaagt aaagaaaaat
      841 tccagcgttg atgtttcagc tgatactcca ggaagcacta tggatgctaa aacagcaaaa
      901 aaatattaca acgtagatcc taaaaaatat ctaaaagatt ggaatttaac tgttcacttc
      961 ggaacaacaa caccttttac tgacatcaga agctatgact ggtcaagaca attaaaaaag
     1021 ccaagtgagc tacaatgggg cgctggttta ggtttgacaa aaatgtttaa tagtgcattt
     1081 ggtttaaatg ttgattatac tttaggtaaa ttattaggta gaacagtaga acgtggtggt
     1141 tttgctgaag aaagacaata ttggaaacaa cttttccctg aacgtacaga agctgacggt
     1201 ccagtatatt tcaaaactaa tgtattccat caagcttcag ttaattttta tattgactgg
     1261 ttaggattag gaacagcata taacaaattt atcaaatcac aaattacagg caagcctatc
     1321 aaatctagaa aatttgcatt ctatactaaa ttaggtatcg gtattattcg tgcttcttct
     1381 caaatttata attcaaaaga tgataagcct attgctaata atgcttactt aagaggttat
     1441 acaaacaaat ttacagaagt agtttttcct atggcttttg gtatgaaatt taaagtttct
     1501 aaatcttttg acattggttt agaaggtaat ttcactttca caaactctga caaattagat
     1561 gcattgcaat tccaatctcg cgtagatgca ggtggcaaaa cggttaactc tttatcaaaa
     1621 ataaacagag atgcttatgc ttatgtgaat gtaaacttct cttacaaatt tggtagaatt
     1681 ggttctcaaa aagaacatgt tgaatgggtt aatccaatcg cattcatcat ggcaaacgta
     1741 ccaaaaccaa aagaagttaa cttaaaagat acagatggcg atggcgtttt agacatctta
     1801 gataaagaac ctacaacaga acctggttta gcagtaaacg ttcatggtgt aacattagat
     1861 agcgacaaag atggttgccc agacagcaaa gatccagagc cattctcatc tcctgctttc
     1921 ccaatcgtag aatgcaaaaa tgtattacct gaagctcctg ctgtaacaga acaagtagtg
     1981 aaaggtggcg ttccttatga tgatgaagca gtaaacaaaa aaatacaaaa agttgaagat
     2041 aatatttgga aattaacttc agtatatttc gatgtaaaca aatacaacat cacttccgca
     2101 ggatctgaag agttgaaaaa aatcggtatc atcttatcga caaatccaaa cttaaaagta
     2161 aatgtaaaag gtaatactga cgttagaggc tcaacagatt tcaaccaaaa attatctgaa
     2221 aatcgtgtta acgcagctat taaatactta aaagatcatt acaatattgc agatgacaga
     2281 ttcgtaaaat taccaatggg caaatctgat ccattagtaa aagatgctgt aaacgaaaat
     2341 caacatcagt ctaacagacg tgttgacttc tctccagttc gatagtcatt aaacaatact
     2401 ataaaaaacc tgttagttta ctaacaggtt ttttttatct ttttctatga aaaataaaat
     2461 cttcaaatca aatccagatt tacaatttat cagaaatgat ataaaaggca atttatttat
     2521 tgatggtaag tacgttttaa aagaagaaaa agataagcaa cctcttggaa aatttttaaa
     2581 atggataagc tccaaaaatc cacagaaaac agaaaaaaaa caagatacat ttattccaga
     2641 gttaatcaaa aatgatacat tcacgcattc atctattgat aaaatagtat ggttaggtca
     2701 ttcgtcattt tatatacaaa tcaataaagt acgaatactg actgatcctg ttttttatag
     2761 tctaacacca cttttaaaac gtaagcataa attaccttgt acaatcgatg aaataaaaaa
     2821 tattgactat attctattgt cgcatggcca tcgagaccat cttgatgttt ccacattacg
     2881 aaaaatatta ttgtacaatc ctacagttga aatactttgc ccattaggat tcaaaaaaat
     2941 gttggcatca ataggtttta aaaaaataca agaagccgct tggtggcaac aatacattgt
     3001 caataaatta gacatcacct ttttacctgc taaacattgg aacagacgat ttataacaga
     3061 ttataacaaa acactttggg gaagctttgc tattaaaagc aaaaatacgt cgctatactt
     3121 tgctggcgat accggatttg atgcacactt tgaagaaata cacaaatatt tctcctcttt
     3181 tgatttttgt ttaatgcctg ttggcgccta taagccaaga tatattatga aatgggcaca
     3241 cgtttcacca gaggaagctg tacaagcttt caatattttg catggcaaaa tatttatacc
     3301 tatgcattat ggcacattcg acttaagtga tgaaccggcg tcagaacctg taaaaatcct
     3361 aaaacactta gaaagagaaa atgaaatcaa tggcgatata gaaatcttag ctgttggcga
     3421 agaattttat atcaaacact aagcttcgtc gtaattaatc gtacaatttg tctttggatg
     3481 tgattgacaa gttaaaataa aaccttcatc gagttcttct tctgtcaaag gctcatcatc
     3541 atccatttct acttttccat ctattaattt tgctttacag ctacaacatg cagcaacgcg
     3601 gcaagcataa ggtggatcaa tatttgcatt aatagctgcc tccagaattg tttctccttc
     3661 ggctacttta atatcgtatt tattaccgtc taaaattact tttacttcgt aagacatatc
     3721 atttacttat taaatcaaaa atagtgattt catgcaaaca tgcaacattt taagtgtata
     3781 agttttaatt aattaacgtg tgattagcat ataaagtgaa gccactaatc aagtggaaaa
     3841 aatatgataa aatcaacgct ttctaagtta ttttttgact taatcaaaat aattattcgc
     3901 ataatctgac aaatttttat tatttcttat ctttatgcat acaaaaaagc atatgcaaac
     3961 taccattatt cgaaacggaa tttactttga tggcttaggc aataaaggca ttaaaaaaga
     4021 tgtcatcata aaagacaata aaatcgaggc agttgtcgat aaagcaccca atattgaaaa
     4081 tgcaaaagaa attgatgcaa ctgataaatg gataatgcca ggtttacttg atatccatgc
     4141 gcactacgat gcagagcttg aagtattacc agctttagaa gaatctgttc gacatggcgt
     4201 aactacagta attattggca attgctcctt atctgctgct ttaggtaaag aagaggaaat
     4261 tgttgattta ttcgcacgtg tcgaaaatat tccagcaaaa gtattgagcc aatggatgat
     4321 tggcaatatt aaatggaaaa atgttcgtga atattatgag catttagata ctgtgcctgt
     4381 tggtcccaat gtggcttcgt ttttaggaca ctccaacttg cgtatgcatg taatgggctt
     4441 ggatagaagc tttacacaaa aaaatgcctc tgaagacgag ctaaagaaga tggaggctat
     4501 cgtagaagaa gccttagatg ctggttattt aggcgtatct atcgatatgt tgccatttca
     4561 tcgttgggct ggtgtgtatg ctccaaaata cactggattt tctgtgcctt cacaattagc
     4621 agcaaaatcg gaatacaaac gactagcaaa cgttttacgc aaaagaaatc gcatattgca
     4681 aattacgcca aatgcgattg aaaaaacgtc atttatgaca atcacaagat taagtcaagg
     4741 tggatttttt aagaaaagtt tgaaaacgac tatcgttgca gcattagatt taaaatcaaa
     4801 tgagaaaata tacaatttac tcaaactatt aggttttatt ggcaataaaa tacttcgagc
     4861 tgatttgaaa tttcaaacat tatcggttcc atttttaaat tatggagatg gcccagtgtc
     4921 tccattgttt gaagaatttc catcgatgat aaaagctata tctgccgacg cagcaggcag
     4981 acatgccatg tttactgatc caacattcag aaaacaattt gtgaaagaat ggaatcataa
     5041 agcggcttct gttttcccaa aagcattaaa agaaatgtgg attgtaaact cgccagtcag
     5101 cacacacata ggcaagaatt ttgaacaagt cgctaaagaa gcaggcgtag aacctgttga
     5161 gcattttatg aatcttttgg cagaatatga taccaaaatc agatggaaat gcgaaacatc
     5221 taatcatcga gaaaatgtac gaatggaatt attgaacagc gaacattgtt ttcctggctt
     5281 caatgactct ggcgcacata atttaaatat ggcatttcac gatggtggat tgcatttatt
     5341 taaacaagct atgcgtcatc ctgaaacaat gccaattgaa cgagctgtgt ataggttgac
     5401 aaaagaacct gctgattatt taggtatcga tgtcggcgat atttcagttg gcaaacgtgc
     5461 tgaccttatt atcattgatc ctacacgatt ggaaagtgat ttaacaacag aacctattga
     5521 agattatcat ccacaatttg aaggttcata tcgattaata aaacgcagtg gtaaagttgt
     5581 acaacatgta tttatcaatg gcaatgaagc ttatacaaat aatggcgaag taaaattcca
     5641 tgaagacttg ggcaagaaaa aaatatttgg tcgattgcta cgcagtaatt ggaattaaat
     5701 aggaaaaaat atatagcttt ttattcgcta ataatttcat taaaatacaa aaaataaaaa
     5761 ggtcaactcg agaagttgac ctttcaattt taattacatc taatttttag aagccttgtt
     5821 tagcaacacc atctttaata gcttgcaatg cttttgcttc acctaaaata gcggccattt
     5881 tattgccttt accatcattt ccagatgcca tataaccacc tcttgatgtt tttgtaatta
     5941 ctgcatcgtg cattggtaca tctttttctt tcgttttaag acaatatgcc ttaatcattt
     6001 ttgatttgtc cattgttgat ttgtttaaag attatcaata tgtctcaaat ttaataaact
     6061 aagttacatt cgtacgtttt cttgctgtta taaataatct actaacattt gatattttta
     6121 acaaaacatt acaacatatt gataatcaga tatttattaa aatactgtat tattcagcta
     6181 aaatattgtt cataattttt gtttgatgac ggatagattc ttggtgaatt g
//
LOCUS       DAOQQV010000138         6117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5523_length_6117_cov_7.519960, whole genome shotgun sequence.
ACCESSION   DAOQQV010000138 DAOQQV010000000
VERSION     DAOQQV010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_6117_cov_7.519960"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLL59_11205"
     CDS             complement(<1..780)
                     /locus_tag="PLL59_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24419.1"
                     /translation="MHKVSDYLSKQLNSEVKIDSVSIDFIRTVELYNVYLSSQKSKTD
                     TILFAKKLDVDLMLGRTLFEQIAKLRDSKIYIDNVALDGVYFYCYRAKADSLYNFQFL
                     LDQFKSNKKKETKKKAGKPLELRLNKLTLSNSNIILDDPHTDKRMDIRFSKIFVDVRE
                     FNISPLKIDAKELELIDPFYKLTDYNEINKPKDPNKPPGKGFNVEGLGKKLNITVDKL
                     TMRNGNHAMDFKKKNQAAGTFLISQMNIHDINIDIENYRWDS"
     gene            940..1947
                     /gene="tsaD"
                     /locus_tag="PLL59_11210"
     CDS             940..1947
                     /gene="tsaD"
                     /locus_tag="PLL59_11210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HMY24420.1"
                     /translation="MPETIILAIESSCDDTSAAICIDGKIVSNLTANQKVHELYGGVV
                     PELASREHTKNIIPVVAEAIKKANITTKDISAVAFTRGPGLMGSLLVGGMFAKSFAQA
                     LHVPLIDVHHMQAHVLAHYIDEPKPTFPFLCLTVSGGHTQIVLVKDYLEMEVIGATID
                     DAAGEAFDKTAKMLKLPYPGGPLIDKYAQLGNPKRFKFSEPQIENLDFSFSGFKTNVL
                     YFLQKEIKNNPTFIEDNLNDICASVQHTIVSILMKKLKKAAEQTDIREIAIAGGVSAN
                     SGLRQALLQSGEKYNWSTYIPQFQYCTDNAGMIAITAYHKYLQNKFCDIDIPILARML
                     M"
     gene            2022..2459
                     /locus_tag="PLL59_11215"
     CDS             2022..2459
                     /locus_tag="PLL59_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMY24421.1"
                     /translation="MQRVSIDFPSPILYSFTFTINADDINIADHMGNERILVLANTLR
                     EKMFEHLQIPLFDLQNRHGIVVANHCIQYKSEGFLGDEILLNAAVNDITECSFDLYFQ
                     FLKNNGKTMALLKTGCVYFDFETRKIMALPESYLRAFHKNISI"
     gene            2627..4249
                     /locus_tag="PLL59_11220"
     CDS             2627..4249
                     /locus_tag="PLL59_11220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HMY24422.1"
                     /translation="MSFSARNRKQYIKQLQSDQFDLLVIGGGITGAGIALDAVTRGLK
                     VCLIEKADFASGTSSRSTKLIHGGLRYLKQLEFGIVREVGQERAILYKNAPHIVHPER
                     MLLPIIEGGSLGKYSTSLGLYVYDRLAGVEKGERRNMLSKQETLAKEPLLNTDILKGG
                     GMYVEYRSDDARLCIEVLKTAVDKGALCVNYVSFSSFTLDNNNKINGAVCLDILNKES
                     LQINAKKVINACGPWVDDLRKIDKSLTKKRLHLTKGIHIVVKKERIPIQQALYFDVQD
                     GRMIFAIPRGNKVYIGTTDTNYTGDVNYPFATKEDVEYTLHAANYMFPNQHLSIDDVE
                     STWAGLRPLIHEDGKSPSDLSRKDEIFISDSNLISIAGGKLTGFRKMAERSVNVVCKQ
                     LKLEDQREFLPCSTANITLSGGHLQQTPLEFAKNLQEDFKQFYLDEILELVLKYGSNT
                     QYILAMAQTNFNNNLLFAEADYCIEHELTNSIADFIIRRTGRLYFERSEMNKQLPSLS
                     SFFQQQIQLPTEVDEMFAKQFDIEYQGVLSFK"
     gene            4347..5978
                     /gene="pruA"
                     /locus_tag="PLL59_11225"
     CDS             4347..5978
                     /gene="pruA"
                     /locus_tag="PLL59_11225"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HMY24423.1"
                     /translation="MPNKIATIPLPTNEPILAYLPNSTERINLKKEISRRKNIVYDIP
                     MIIAGKEVRTNDTVDIFPPHELSHKLAHYHKGNTTHVQQAIDAALQAREKWASMHWED
                     RASIFLKAADLIAGPYRAAINAATMLGQSKNVYQAEIDSACELADFLRFNVYFASQIY
                     NEQPISSPGTRNTLEYRPLEGFVYAVTPFNFTAIAGNLSVSPALMGNVVVWKPSNTQI
                     YSAWTIMEIFKEAGLPDGVINMVFANAAETTNLILSHPFFAGVHFTGSTGVFNSIWET
                     IGKNLHIYKSYPRIVGETGGKDYILAHQSADIDVLATALLRGAFEYQGQKCSACSRAY
                     IPKSIWDTTWEKMYAALKTFKIGNVENFTVIMGAVISEDAFKRITRYIEQAKANPDNI
                     IMYGGNYDNKKGYYIEPTVILTKDPKSATMVEEIFGPVLTIYVYEDETFDDILHLVDN
                     TSEYALTGAVLAQDTYIIEKMRNRLVNSAGNFYINDKCTGAVVGQQPFGGARKSGTND
                     KAGSKWNLLRWVSPRTIKENYVPPKYYSYPYMDEE"
BASE COUNT         2089 a          952 c         1060 g         2016 t
ORIGIN      
        1 agaatcccaa cggtagtttt ctatgtcgat attgatatcg tgaatattca tttggctaat
       61 cagaaaagta ccagcagctt gatttttctt tttaaaatcc attgcatggt ttccatttcg
      121 catcgttaat ttatcaaccg taatatttag tttttttcct aagccttcta cattaaagcc
      181 ttttcctggt ggtttattag ggtctttagg tttgttgatt tcattataat ccgtaagctt
      241 ataaaatgga tcaataagtt ctaattcttt tgcgtctatt tttagtggac taatattaaa
      301 ctcacgaaca tctacaaata ttttagagaa tcgaatatcc attctcttgt cagtatgtgg
      361 atcatctaaa ataatatttg aattgctaag tgtaagtttg ttcaaacgca attctaatgg
      421 cttgcctgct ttctttttag tttctttctt tttattggat ttaaattgat ctaataaaaa
      481 ttggaaatta tacaagctat ctgctttcgc acgataacag taaaaataaa caccatctaa
      541 agcaacgttg tctatgtata ttttgctatc tctgagtttt gctatttgtt caaataaagt
      601 tcttccaagc attaaatcga catctaattt ttttgcaaat agaatagtat ctgttttgct
      661 tttttgagat gaaaggtaaa cgttgtataa ttctactgtt cttataaaat ctatactaac
      721 agagtcaatt tttacttcac tatttaattg ttttgataaa taatcactta ctttgtgcac
      781 taaataagtt tgtacttttg gttgttgtat taggtaaaag acggaaacaa ataacaatag
      841 gagtattaag acaatagtca atacagctcg aaatgtatat ttaaagattt ttttcaacga
      901 tataaaaatt gcaaaattaa gcgaaaataa gcaaatatta tgcctgaaac tatcatatta
      961 gcaatagaat cttcttgtga tgatacatca gctgcaattt gtatagatgg taaaattgtt
     1021 tccaatttaa cggccaatca aaaagtacat gaattatatg gtggtgtggt gccagaatta
     1081 gccagtcggg aacatactaa aaatattatt cctgtcgttg ctgaggcaat taaaaaagca
     1141 aatattacta caaaagacat cagtgcagtt gcgttcacac gaggccctgg attaatgggt
     1201 tctttactcg ttggcggcat gtttgcaaag tcatttgcac aagcgctaca tgtgccatta
     1261 attgatgtac accacatgca ggctcacgtt ctggctcact atattgatga gcctaaacct
     1321 acgtttccat ttttatgtct tactgtttct ggaggacaca cacaaattgt acttgtcaaa
     1381 gattatttag aaatggaagt cattggcgca acaatagatg atgcggcagg agaggccttt
     1441 gataaaacag caaaaatgtt gaaactacca tatcctggtg gtcctttaat agataaatat
     1501 gcacaattag gcaatcctaa aaggttcaaa ttctcagaac cacaaattga aaatttagat
     1561 ttttctttta gtggtttcaa aacgaatgtc ttatattttt tacaaaaaga aataaaaaat
     1621 aatcccacat ttattgaaga taatttgaat gatatctgtg cttctgttca acatactatt
     1681 gtctctattt tgatgaaaaa attaaaaaaa gcagctgaac aaacagacat aagagagata
     1741 gcaatagctg gaggtgtgag cgctaattct ggattgcgac aagcattgct acaatctggt
     1801 gaaaaataca attggagcac atacattcct cagtttcaat attgtacgga taacgctgga
     1861 atgattgcta taactgcata tcataaatat ttacaaaata aattttgtga tatagacatt
     1921 ccgattcttg cacgaatgct tatgtaatta tattgttttt attttcttca taagcaaaaa
     1981 tgtgtacttg acattattta tcctaatatt gtataatatt tatgcaacgc gtttctatag
     2041 attttccttc tccaatccta tattctttca catttacgat aaatgcagat gatataaata
     2101 ttgccgatca catgggcaat gaacgcatct tagtattagc gaatacactt cgtgaaaaaa
     2161 tgtttgaaca cttgcaaatt ccacttttcg atttgcaaaa cagacatgga attgtagtcg
     2221 ccaatcattg cattcaatat aaaagtgaag gatttttagg tgatgaaatt ttgcttaatg
     2281 cagccgtcaa tgatatcaca gagtgcagtt ttgatttgta ttttcagttt ttaaaaaata
     2341 atggaaaaac tatggctttg ctcaaaactg gttgtgtata tttcgatttt gaaacacgaa
     2401 aaataatggc tttgccagaa agttacttgc gagcatttca taaaaacatc agtatctgaa
     2461 ttgcaattaa tactttataa tcaatatctt tcattccatc accatatatc ggtactttca
     2521 taatgctgaa agtttaagct tatattattt attgttaatt caatattttt gagctaagca
     2581 atttttactt tcacttatct ttagataatt ataattcaaa gtttgtatgt cattttcagc
     2641 tcgcaatcgg aagcaatata ttaaacaact acaatccgac caatttgatt tattggtcat
     2701 tggtggtggc atcactggtg ctggaatcgc attagatgcc gttacacgtg gcttaaaggt
     2761 gtgtttaatc gaaaaagcag attttgcatc aggcactagc tcgcgttcta ctaaactaat
     2821 tcatggtggt ttacgctatt tgaaacaatt agaatttggt atcgttcgcg aagtaggtca
     2881 ggagcgtgct attttataca aaaatgcacc acatattgtt catccagaaa gaatgttatt
     2941 gccaataatt gaaggtggtt cattaggaaa atattcgact tcattgggtt tatatgttta
     3001 tgaccgatta gctggcgttg aaaaaggaga acgaagaaac atgctttcta aacaagaaac
     3061 tttagcaaaa gaaccattgc taaacacaga tattttaaaa ggaggtggca tgtatgttga
     3121 gtatagaagc gacgatgctc gcttgtgtat tgaagtactg aaaactgctg tagataaagg
     3181 tgcgctgtgt gttaattatg tttcattttc atcattcaca ttagataata ataataaaat
     3241 caatggcgca gtttgtttag atattttaaa taaggagtca ttacaaatta atgcaaaaaa
     3301 agtaattaat gcttgtggcc cttgggtcga tgacttgcgt aaaatagaca aatcattaac
     3361 aaaaaaaaga ttacatctta caaaaggtat tcatatcgta gttaaaaaag aaagaatacc
     3421 aatacaacaa gccttatatt ttgatgtaca agacggtaga atgatttttg cgattcctcg
     3481 tggtaacaaa gtgtatattg gtacaacaga tacaaattat actggcgatg tcaattatcc
     3541 atttgctaca aaagaagatg tagaatatac cttgcatgct gctaattata tgtttcccaa
     3601 tcagcattta agcattgatg atgtggaatc aacttgggct ggtttaagac cattaataca
     3661 tgaagatgga aaatcaccat ctgatttatc gcgtaaagat gaaattttta tttcagattc
     3721 taatctaatt tctattgctg gtggtaagct tacaggattt cgaaagatgg ctgagcgtag
     3781 tgttaatgtg gtatgtaaac aactaaaatt agaagaccaa cgagaatttt taccttgcag
     3841 tacggctaat attacattaa gtggtggaca tttacagcaa actccattag aatttgctaa
     3901 gaatttacaa gaagatttta agcaatttta tttagatgaa atacttgaat tagtgttgaa
     3961 atatggcagt aatacacaat atattttagc tatggcacaa actaatttca ataataattt
     4021 actattcgca gaagcagatt attgcataga acatgaactc acaaattcta ttgctgactt
     4081 tatcataaga cgaacaggta gattgtattt tgaacggtct gaaatgaata aacaattacc
     4141 atcactaagc tcattttttc aacaacaaat acagcttcca acagaagtcg atgaaatgtt
     4201 cgctaaacaa tttgacatag aatatcaagg agttctatcg tttaaataaa gttgttataa
     4261 caactaatta cttatttcaa cttttatatc gtaaatttgt tttcaaatag ttactgtttc
     4321 taattgttaa taaataaaat atagctatgc ctaataaaat tgctaccatt ccattgccta
     4381 ctaatgaacc tattttagct tacttgccca atagtacgga acgtataaac ttgaaaaagg
     4441 agattagcag acgcaaaaat atagtgtatg atattccgat gattattgca ggcaaagaag
     4501 tgcgcaccaa tgacacagta gatatttttc caccacatga attgtcacac aaattagcgc
     4561 attatcacaa aggaaataca acacatgtac aacaagctat agatgcggca ttgcaagcca
     4621 gagaaaaatg ggcaagtatg cattgggaag accgtgcatc tatcttttta aaagctgctg
     4681 atttgattgc tgggccttat cgcgctgcta ttaatgcagc aacaatgcta ggtcaatcga
     4741 aaaatgtcta tcaagcagaa attgactcag cttgcgaatt agctgatttt ttacgtttta
     4801 atgtttactt tgcttcacaa atatataatg agcaaccaat ctcatctcca ggcacaagaa
     4861 atactttaga atatcgtcct ttagaaggtt ttgtatatgc agtaacacca tttaatttta
     4921 cagcaattgc aggtaattta tctgtatcgc cagcattaat gggcaatgta gtcgtatgga
     4981 aaccatctaa cacacagata tattcagcgt ggactatcat ggaaattttc aaagaagcag
     5041 gtttgcctga tggtgtcatt aatatggtat ttgcaaatgc tgctgaaaca actaatctta
     5101 ttttgtcgca tccttttttt gcaggtgttc attttacagg ctctacaggt gttttcaata
     5161 gcatttggga aaccataggc aaaaatttac atatctataa aagctatcca agaatcgtag
     5221 gcgaaacagg cggtaaggat tatattttgg cgcatcagag tgccgatatc gacgtattgg
     5281 caactgcatt acttcgcggc gcttttgaat atcaaggtca aaaatgctca gcttgttcac
     5341 gtgcctatat tccaaaatcg atttgggaca caacttggga aaaaatgtat gccgcattaa
     5401 aaacttttaa aatcggaaat gtcgaaaatt ttactgttat catgggcgct gttatttctg
     5461 aagatgcatt taaaagaatc acaagataca tagagcaagc aaaggccaat ccagataata
     5521 tcattatgta tggtggaaat tatgacaaca aaaaaggcta ttatattgag cctacagtga
     5581 tattgacaaa agatccaaaa tcagcaacga tggtggaaga aatttttggt cctgttttaa
     5641 caatttatgt gtatgaagat gaaacatttg atgatatttt acatttagta gataacacat
     5701 ctgagtatgc acttactggt gctgttttgg cgcaagacac ctatattatt gaaaaaatgc
     5761 gaaacagatt agtaaatagt gctggtaatt tttatatcaa tgataaatgc acgggtgctg
     5821 tggttgggca acaaccattc ggtggtgcac gtaaaagtgg tacgaacgac aaggctggca
     5881 gtaaatggaa tttactacga tgggtttcgc cacgcacgat aaaagaaaac tatgtgccac
     5941 ctaaatacta ttcctatcct tatatggatg aagaataaaa gcattaaaaa taattacaac
     6001 aaagccaata tgaagtacat gttggctttt taaaaatacg ttacacatga tgcatatatt
     6061 gattgttttc taaaaaaata ataggagatg ccgttcctga atattggaaa ataatag
//
LOCUS       DAOQQV010000139         6053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5615_length_6053_cov_7.575692, whole genome shotgun sequence.
ACCESSION   DAOQQV010000139 DAOQQV010000000
VERSION     DAOQQV010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5615_length_6053_cov_7.575692"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1154)
                     /locus_tag="PLL59_11230"
     CDS             complement(198..1154)
                     /locus_tag="PLL59_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMY24424.1"
                     /translation="MNFFYTLLFSFISCFGFAEGMHISGIITDTESQKKIEGAFVLIH
                     FYNGFEFSDITDSTGRYDIYTSAVVPEDYYTIQIDAKNYYQLNGFVHVTKNCVFNFNL
                     KSKNQTQQVVSIPIIDAVKPTLPMPALEGFATNNLVFLIDASSSMNMQEKMPLLKTAM
                     KYLVNELRTTDKISILTFSSAVKEILPATTLNDKQNILSLIDNISFGSSSQGGLALQK
                     AYKTAQDNFIKKGNNRIILATDGLFTSGEKDYKKMQQLIETGLDKNISLSIFCFGKNT
                     EYVNTKLSKLANIGNGNFATITDAENGKHYMLEEAKAVKVEK"
     gene            1233..1982
                     /locus_tag="PLL59_11235"
     CDS             1233..1982
                     /locus_tag="PLL59_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMY24425.1"
                     /translation="MKRIYIILLCLLIVLQASAQDSSARKSVYKFRLSVDIPIGLMSA
                     GFGIGALIKHKKNKPLTLADVSNLKPSDVNKFDRYAVYQHSKAAAISSDVFQYTAMLS
                     PAFLFIDKDIRKDWKTILPIWAETFAFTSVLTMFTKEMVRRKRPYVYSEEYNGNKISK
                     SAYASFFSGHTSVTAASTYFIAMVYSDYHPTSKWKPLVWTGAAVFPALTALMRVKAGK
                     HYWTDVITGYAVGALVGTLTPYLHRREFRRK"
     gene            complement(1969..2637)
                     /locus_tag="PLL59_11240"
     CDS             complement(1969..2637)
                     /locus_tag="PLL59_11240"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMY24426.1"
                     /translation="MNAIIKIGSYIFDLPVEKKNSNYSGEVVVSIHEGQYKLSTQNAI
                     YSFGKKYTSFDIAFQAIQVQQRNINEVLVLGFGLGSVVDLLEKQNTISSIIAVDIDEV
                     IIDLAKKYLHTPLKSKVKFICADANDYVSQETNKFDMLIVDLFIDDETPLPFMQADFL
                     RKLQNLLNKDGVLLFSKIDDLQKHKIENIQFEQLFQQQFPNGFSIDAKGNKIFAWINS
                     PTYV"
     gene            complement(2638..>6053)
                     /locus_tag="PLL59_11245"
     CDS             complement(2638..>6053)
                     /locus_tag="PLL59_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24427.1"
                     /translation="FSKSKDVKVYRDLKPLAKQPFTSEYHKMFIYADLLRWNLDSTII
                     KFSMITLSGDIPAIFESFNYYQPNLENKYKGPSEQGPIDKIYRYYESNLGNRFVDGQS
                     LALDINPGAPFSATQSIFFKLVEDGYINYNPSTRMIEVKEKLVNQALASKGKQDYDFI
                     KFASFKRNLNARLDIKSNIMEVYGVEEINMSTKSGVKFIPNNDTVRISKNRVMSLGGK
                     IQVGNFDFVAKKVDFDYDKYAFNMKNVDSMVVYVPETDKPDEKGRIKLIRSKTPIQNI
                     TGTLHIAEPNNKSGTNNNLKYPYFSSGDTAKVAYDKGANGDKYNADKFFYQVYPFELD
                     SLNTLNTEKLQLNGQLISGGIFEPIKSGLKLQNDKSFGIDVNTGEKGYNLFGNKGKYT
                     AALKLDASGLSGKGLFEFGPVKLYADTTFFFMDSVYADLDSVRITEDKVANLPQTKIN
                     LSSFAWNVKKDSLVISEGKEERFSMYNGTTDFDGKLILHNKNLNGVGVLSWNHTKLQS
                     DDITFKARKFEAKNGKLNLSTDEGLSLLASNDVNAKFDLDNKIADIELNKNDTIPLES
                     FKYVANPKFLHFDIAKNMLTLNAATKYSKFFLLSTDRTKDSLKFVTAEAELNLNDNSI
                     HFAGINELRLADSKVIPDKGEIFIEQDGTVRSLRNAIVVFNADSNYHQVKDAIINIVG
                     RNDFSGTGNYYFKMNNGTVEKVPITEINVNNPYRGQVLPEAKKGKKHADNRDLSKIYT
                     YANTDIEEVASFKIDNKIYYKGNFSFDSKHKEIFLDGYVKIDIANNSSDWIPNVQTLD
                     PKKPAVSMDSILSQADNNLFVGLMLDKSVPEFYSAIIQDKRSVSDASIMSVKGSMAYS
                     KTEPGVINFGDESAFTSAYSRHSCIKYNENTRELNASGEIGFGLRLKPCKVTTVGTFT
                     FTPNKQNLVITSDLAVKFMIQPAIASSILAPFLVAEETASFTNYKRNKTIHRTLSVLT
                     KDSLESNRLIGSIYSMDSMFIPQSLDYNMLLSGSKFYWDEQDATFKSVEKVSLAFFGQ
                     DVVKRQYDAYIEIGYAYESDFVNIYLQSKSGAWMYFKIKRGQMGIASSVADVYNTITL
                     LKDSDRVYREGKEVLFEFMPADMAMRDNFVARMEDFMERFKLNTAPAPK"
BASE COUNT         1890 a         1067 c          946 g         2150 t
ORIGIN      
        1 gtaagcttat aagtcgactt attgatggca tgattttcag cattgccgat ttgaaaaaca
       61 aataataata aggtgaaaag tataaagact tttgatttca tttatgtcgt caaaagtaaa
      121 taagtcaaac aaaataatta tttacaaatc attaagaatt gttatcaatt ttaattttta
      181 ctatatttta acaatgctta tttttcaact ttcacggctt tcgcttcctc aagcatatag
      241 tgtttaccgt tctctgcatc agttattgtt gcaaaatttc cgtttcctat gtttgccaac
      301 ttagagagtt tagtgtttac atattctgta ttttttccaa aacaaaaaat agaaagtgat
      361 atatttttat ctaatcctgt ttctattaat tgttgcattt ttttataatc tttttcacca
      421 gaagtaaata aaccatcagt tgctaaaata attctattgt tgcccttttt aataaaatta
      481 tcttgtgctg ttttatatgc tttttgcaaa gcgagtccac cttgtgaaga actaccaaaa
      541 ctaatattat ctattaagct taaaatattt tgtttgtcat taagcgtggt tgctggtaaa
      601 atttctttta cagcactaga aaaggtaagt atagaaattt tatccgtagt gcgcagttca
      661 tttactaaat atttcattgc tgttttcaat aatggcattt tttcttgcat attcatcgaa
      721 gaagatgcat caataaggaa taccaagtta ttcgttgcaa agccttctaa tgctggcatt
      781 ggcaatgtag gttttactgc atcaattatt ggaattgaca ctacttgttg tgtttgattt
      841 ttacttttta ggttaaaatt aaacacacaa ttttttgtta catgtacgaa accatttaat
      901 tgataataat ttttagcgtc aatttgtatg gtataataat cttctggcac aactgctgat
      961 gtatagatgt catacctacc tgttgagtca gtgatatctg aaaattcaaa gccattataa
     1021 aaatgaatta agacaaaggc accttctatc tttttctgtg attctgtgtc tgtaataatt
     1081 ccgctgatgt gcataccttc agcaaaacca aagcaagaaa taaagctaaa taaaagcgtg
     1141 taaaaaaaat tcatctacaa aaatttagta aatataaaat tatacattgt taattaaata
     1201 cacacaacaa aatttatgct aattttaacg ccatgaaacg tatatacatc atattgcttt
     1261 gcttacttat cgtattgcaa gcatcggcac aagattcttc tgcacgaaaa agcgtgtata
     1321 aatttcgatt aagtgtagat ataccaattg gcttgatgag tgcagggttt ggtattggcg
     1381 cattgattaa acacaaaaaa aacaagccac tcactttggc agatgttagc aatttaaaac
     1441 ctagtgatgt caataaattt gacagatatg ccgtctatca acattcaaaa gcagcagcca
     1501 tctccagtga tgtatttcaa tatacagcga tgctaagccc tgcattttta tttattgata
     1561 aagatattcg taaagactgg aaaactattt tacccatttg ggcggaaaca tttgcattta
     1621 catctgtatt aacgatgttt acaaaagaaa tggtgaggcg caaacgtcct tatgtctatt
     1681 cggaagaata caatggaaac aaaattagca aaagtgctta tgcttcattt ttttcaggac
     1741 atacatctgt caccgcagcc agcacttatt ttatagcaat ggtgtatagc gattatcatc
     1801 cgacatcgaa atggaaaccc ttagtttgga ctggcgctgc tgtatttccg gcacttaccg
     1861 cactaatgcg cgtgaaagca ggcaaacatt actggactga tgtgattaca ggttatgctg
     1921 ttggcgcatt agtgggcacg cttacgccat atttacatcg tagagaattt agacgtaagt
     1981 aggcgaattt atccaagcaa atattttatt gcctttggca tcaatggaaa agccatttgg
     2041 aaattgttgt tgaaataatt gttcaaattg tatgttttca attttatgct tctgcaaatc
     2101 atcgattttt gaaaataaaa gaacaccatc tttgttcaat aaattttgaa gctttcgcaa
     2161 gaaatctgct tgcataaacg gcaatggtgt ttcatcgtca ataaacaaat caacaatcaa
     2221 catatcaaat ttatttgttt cctgacttac ataatcatta gcatcggcac atataaattt
     2281 gactttactt tttaatggtg tgtgtaaata tttttttgct aagtcaataa tcacttcgtc
     2341 aatatctact gcaataatag agcttattgt gttttgtttt tctaataaat caacaacgct
     2401 gcctaagcca aatccgagca ctaaaacctc gtttatattt cgttgctgta cttgtattgc
     2461 ttgaaatgct atatcgaatg aagtatactt ttttccaaaa gaataaatag cattttgagt
     2521 gctcaattta tattgacctt catgaataga aacaacaact tcaccactgt aattactgtt
     2581 tttcttttcg acaggtaaat cgaaaatata acttcctatt ttaataatgg cattcaacta
     2641 tttcggtgct ggtgctgtgt ttaatttgaa acgctccata aagtcttcca ttcgtgctac
     2701 aaaattgtcg cgcatggcca tatctgctgg cataaattca aacaacactt ctttgccttc
     2761 gcggtaaact ctatcactat ctttcaataa agtaatcgta ttatatacat cagcaacaga
     2821 agaagcgatg cccatctgac cgcgtttaat tttgaaatac atccatgcac ctgatttgct
     2881 ttgcaagtaa atattgacaa aatcgctttc atacgcataa ccgatttcaa tataggcatc
     2941 gtattgacgt ttaactacat cttgtccaaa gaatgccaag cttacttttt ctacgctttt
     3001 gaatgttgcg tcttgctcgt cccaataaaa ttttgaacct gacaggagca tgttataatc
     3061 taaactttgc ggaataaaca ttgagtccat cgaatatatg ctgccaatta atcgattgga
     3121 ttccaagcta tcttttgtca gcacagacaa tgttctgtgt atcgttttat tgcgtttgta
     3181 atttgtaaaa cttgccgttt cttcagcgac taagaatggc gcaagaatag aactagcaat
     3241 tgctggttga atcataaact tgactgccaa gtcagatgta atcaccagat tctgtttatt
     3301 tggtgtaaag gtaaatgtac caaccgttgt aactttacaa ggttttaatc gcaatccaaa
     3361 tccaatttca ccagacgcat ttagttctct tgtattctca ttatatttta tacaagaatg
     3421 acgtgaatag gctgaagtaa atgcgctttc atcgccaaaa ttgataacgc caggttctgt
     3481 tttgctataa gccattgagc ctttaacgct cataatgcta gcatcactca ccgaacgttt
     3541 atcttgaatg atggcagaat aaaactctgg tacgctctta tctaacatta aaccaacaaa
     3601 caaattattg tcggcctgac taagtatgct atccatgctc acggcaggtt ttttcggatc
     3661 taatgtttgt acattcggaa tccaatcgct ggaattatta gcaatatcaa ttttaacata
     3721 accatctaag aaaatttctt tatgttttga atcaaaacta aaatttcctt tatagtatat
     3781 tttattatca attttaaaag aagctacttc ttcgatatcg gtatttgcat acgtgtatat
     3841 tttacttaaa tcgcgattgt cggcgtgttt cttacctttt ttagcttctg gcaatacttg
     3901 tccacgatac ggattattta cgtttatttc tgtaattggt actttctcga cagtgccatt
     3961 attcattttg aaataataat taccagtacc agagaaatca tttctaccga caatatttat
     4021 gattgcatct ttcacttgat gataattgct atctgcatta aatacgacaa ttgcatttcg
     4081 taatgagcgc acggtaccgt cttgctcaat aaatatttca cctttatctg gaataacttt
     4141 tgagtctgca agacgcaatt catttatacc ggcaaagtga atagaattat cgttcaaatt
     4201 taattcggcc tctgctgtaa caaactttaa agagtctttt gttctatcag ttgacagcaa
     4261 gaaaaacttg ctatatttag ttgcagcatt aagcgtcaac atattttttg caatatcaaa
     4321 atgtaagaat tttggattgg caacatactt gaatgattcg agtggaatcg tatcattttt
     4381 gtttaattcg atgtcggcaa ttttattatc taaatcgaat tttgcgttga catcattaga
     4441 ggctaataaa gacaaaccct cgtctgtaga gagattgagt ttaccgtttt tggcttcaaa
     4501 ttttcgagct ttaaaggtaa tgtcatcaga ttgcaatttt gtatgattcc aagaaagcac
     4561 gccaacgccg ttcaggtttt tattatgcaa aattaatttt ccatcaaaat ctgtcgtacc
     4621 gttgtacatg ctaaagcgct cctcttttcc ttcagaaatc accaatgaat ctttttttac
     4681 attccaagca aatgaggata aatttatttt tgtttgtggc aaattggcta ctttatcttc
     4741 cgtgatgcgc acactatcta aatcggcata aacgctatcc ataaagaaga aagtagtatc
     4801 tgcatatagt tttacaggtc caaattcaaa taagccttta cctgacaaac cactggcatc
     4861 taattttagt gctgctgtat attttccttt attgccgaat aagttatatc ctttttcacc
     4921 tgtgtttaca tcaataccaa atgatttatc attttgcaat ttaagtccag attttatagg
     4981 ctcaaagatg ccacctgaaa tcaattgacc attcaattgt agtttttcgg tattcagtgt
     5041 atttaagcta tcgagctcaa atggatagac ttgataaaag aatttgtccg cattatattt
     5101 atcaccgttt gcacctttat cataagcgac ttttgccgtg tcaccacttg aaaaatatgg
     5161 atatttcaag ttattattcg tgcctgattt attattaggc tcagcgatat gcaatgttcc
     5221 tgtaatattt tgaataggcg ttttagagcg tatcagttta attcgtcctt tttcgtctgg
     5281 tttgtcagtt tctggaacat acacaaccat agaatccaca tttttcatat taaaagcata
     5341 cttatcataa tcgaagtcca ctttctttgc aacaaaatcg aagttgccga cttgaatttt
     5401 tccacctaat gacatgactc tatttttgct gatgcgcacg gtatcattat tcggaataaa
     5461 tttaacacca gacttcgtgc tcatatttat ttcttctacg ccatacactt ccatgatgtt
     5521 agatttgata tcaaggcgtg cattgagatt gcgtttgaat gaagcaaatt ttataaaatc
     5581 gtaatcttgt tttcctttag atgctaatgc ttgattaacg agtttttctt tcacttcaat
     5641 cattctcgtc gatggattat agttgatata accatcttct accaacttaa aaaaaataga
     5701 ttgagtggca gaaaaaggtg caccaggatt gatgtctagt gcaaggcttt gtccatctac
     5761 aaatcgattg cctaagttgc tttcataata tctatatatt ttatcgattg gcccttgctc
     5821 gcttggtcct ttatatttgt tctctaaatt cggttgataa taattgaacg attcaaagat
     5881 agcaggtata tcaccagaca aggtaatcat actaaattta ataattgtgc tatctaaatt
     5941 ccatctcaat aaatcagcat aaataaacat tttatgatat tcgctagtaa atggttgctt
     6001 cgccaatggt ttcaaatcac gatatacttt tacatctttt gatttagaaa aat
//
LOCUS       DAOQQV010000140         5983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5727_length_5983_cov_7.075911, whole genome shotgun sequence.
ACCESSION   DAOQQV010000140 DAOQQV010000000
VERSION     DAOQQV010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5727_length_5983_cov_7.075911"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLL59_11250"
     CDS             <1..202
                     /locus_tag="PLL59_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMY24428.1"
                     /translation="QEGAKSYEIYKRNISENNPLKLTVVDIAKGSFLDSDVKKETVYF
                     YSIIAKGQYGISDRSAEKFVKY"
     gene            complement(214..753)
                     /locus_tag="PLL59_11255"
     CDS             complement(214..753)
                     /locus_tag="PLL59_11255"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HMY24429.1"
                     /translation="MENNSLDTNQNNNSVIEIKEIAIRYGIIGGLAGILASLVVYFAN
                     VQYETWSKWLQTLILLLTIVLCVKSIADENKGKEVAFGVLFKGGMLATAVLTAIAIIY
                     FMVYINVIEPDFIDNLLQITKTEMLNKGLSEEQADAAIKISKKFMSPAIMVSITTISS
                     LIIGAIVSAISAAIFKREN"
     gene            complement(793..2022)
                     /locus_tag="PLL59_11260"
     CDS             complement(793..2022)
                     /locus_tag="PLL59_11260"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMY24430.1"
                     /translation="MNSKQKLVVIGGGAAGFFAAITAAENNPDLAIIILEQGNEVLGK
                     VKISGGGRCNVTHACFDVKELVKFYPRGSKELVGPFYTFNCEHTVDWFNHHQVALKTE
                     SDGRMFPVSNRSQTIIDCFIQQCHVLKIEINKHAKVTHIEKQDTYKVFFNDKCINADY
                     ILLASGSSNTIWNMLKKLGHQIISPVPSLFTFTIKNELIKNLEGVSVSNVQCKLLDSN
                     IATSGSILITHTGLSGSSILKLSAFAAREMNARNYQFYIEINWISIRLQDAIQVLKNA
                     KQTFPKKNISNLAVFDLPHRLWQSVLAINGIKYEKNVADLSNNDIQNIAQTLTQSKLQ
                     VTSKNTNKDEFVTAGGIDLKEVDFKTMESKVLDNLFFAGEILNIDAVTGGFNFQAAWT
                     TGNIAGTSIAERSTNYK"
     gene            2131..4704
                     /gene="gyrA"
                     /locus_tag="PLL59_11265"
     CDS             2131..4704
                     /gene="gyrA"
                     /locus_tag="PLL59_11265"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMY24431.1"
                     /translation="MSEELEGLNRPRIVPINIEDEMKSAYIDYSMSVIVSRALPDVRD
                     GLKPVHRRVLFGMSELGMTSNKPYKKSARIVGEVLGKYHPHGDASVYDTMVRMAQAWS
                     LRYTLIDGQGNFGSIDGDPPAAMRYTEARLKKIAEDMIEDLHKDTVDFQLNFDDSLEE
                     PTVMPTRIPQLLVNGASGIAVGMATNMLPHNLSEVIDGIVAYIDNREITIDELMQFIK
                     APDFPTGGIIYGYEGIKDAFRTGRGKVRVRGKARIEENKEGRTQIIISEVPYQIQPKM
                     IITRAADLVNEKKLEGISEIRDESDRDGLRVVFDLKRDAIPNVVLNNLYQLTPLQTSY
                     GVNNIALVNGRPQLLNLKEMIQYYVDFRHEVVTRRTQFELRKAEERLHILEGYLIALD
                     ALDEVIQLIRKSKNPEEAKDGLMNNFGMSELQAKAVLALTLQRLTGLEIDKIRAEHKE
                     VSDLIAKLKEILANEHLRFEIIKEEMLEIKKKYGDERRSEIVLYGDDISYEDMIAEEE
                     MVVTISHLGYVKRTSLDEYKKQNRGGKGSQGGKTRGEDFIEHLFVASTHNYLLLFTEQ
                     GKCFWLKVYEIPEGNKTSKGRPIQNVINIPQDDKIKAFINVEDLKNEEFLDSHYIVMA
                     TKRGTVKKTSMREYSRPRSNGVNAITVREGDELITAALTNGKCEIILGSQKGYAVRFN
                     EEDVRSIGRTGSGVRGITLRGDDDELIGMVCADTSSDIKEDIMVVSENGYGKRSALDD
                     YRITSRGGKGVISLNVTEKTGNMIAIESVTDNNDLMIINKSGLTIRIGLNTLRTMGRN
                     TQGVSLIKLKDDDAIAAVSKIEHEEEEENENVENNHTDTDQSSSNEDNSNQHSEIQNE
                     E"
     gene            4694..5860
                     /locus_tag="PLL59_11270"
     CDS             4694..5860
                     /locus_tag="PLL59_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY24432.1"
                     /translation="MKNNMNTLKLIVLLVSIALYTQSAFAQKGKITSAQLSLQDGKVL
                     DAKKDIDLALANEEIQKRVDAWTTKGEVYTQIYVTKLYYAQNPNCLFDAKAAYLKAFE
                     LETNPKKQKNFSTPLNDLSGYLFNEALNRFNAKKYDDAYTHFDASRTINEFLFSKNLV
                     STIDTNAIYATAMAGANANKIAEVKPLLEKLITMNYDNPAVYETLAQVYETEKNTDAL
                     KKLVSAGLKKYPNSKNLQIYDLNAALDNTDVNESITKFEAALATDPKNASIAFNLGVL
                     YDKVQNTAKTKEYYQKAIELKPDYGDAYFNLGVMYFNAGVVKNKEMNAVDDTKDRDGK
                     IYEALKKERNDLFQLALPNLEKAYAIDPKNIDYKANLKKVYASMNMLDKAKALD"
BASE COUNT         2133 a          937 c         1081 g         1832 t
ORIGIN      
        1 acaagaaggg gcaaagtctt atgagattta taagcgaaat ataagtgaaa ataatccatt
       61 aaaattaact gttgtagata ttgcaaaagg tagttttcta gattcggatg tcaaaaaaga
      121 aactgtgtat ttttattcga taattgcaaa aggacaatat gggatttctg ataggagtgc
      181 agaaaaattt gtgaaatatt aataagcaat aaattaattt tctcttttaa aaatagcggc
      241 gctaattgcg gatacaattg caccaattat taaagaacta attgtggtaa tgctgaccat
      301 gattgctggc gacataaatt ttttacttat cttaattgct gcatctgctt gctcttcact
      361 caatccttta ttcaacattt cagtttttgt tatttgtaac aaattgtcaa taaaatcagg
      421 ttctatgaca tttatataaa ccataaaata gataattgca attgccgtta aaacagcagt
      481 tgctaacatg ccacctttaa acaacacacc aaatgctact tctttacctt tgttttcatc
      541 agctatagat tttacacata acacaattgt taataacaag atgagtgttt gcaaccattt
      601 tgaccaagtt tcatattgca cattagcaaa atatacaaca agtgatgcta aaatacctgc
      661 taatccacca atgattccat atcgaatggc aatttcttta atctcaatta cactattgtt
      721 attttgattt gtgtctaatg aattattttc catcttatgt ttttttcaaa agtaataaat
      781 ctttagcatt gtttatttat aattagtcga acgctctgca atacttgtgc ctgcaatgtt
      841 tccagttgtc catgcagctt gaaaattaaa gccacccgtt actgcatcaa tatttagtat
      901 ttcaccagca aaaaataggt tgtctaatac tttactttcc attgttttaa aatcgacttc
      961 ctttaagtcg attccaccag cagtcacaaa ctcatcttta tttgtgtttt ttgaagtaac
     1021 ctgcaacttt gattgcgtca aagtttgtgc aatattttgt atgtcattat tacttaagtc
     1081 tgccacattt ttctcatatt ttatgccatt tatagctaaa acagattgcc ataaacgatg
     1141 tggtaaatca aaaacggcaa ggttgctaat attctttttt ggaaatgttt gtttcgcatt
     1201 ttttagtacc tgaatagcat cttgtaatcg aatagaaatc caattgattt caatgtaaaa
     1261 ttgataatta cgtgcattca tttcacgagc agcaaaagca gataatttta aaatacttga
     1321 tccacttaat ccagtatgcg ttatcaatat actaccactt gttgcaatat ttgagtctaa
     1381 taatttacat tgaacatttg atacagatac gccttctaaa ttttttatta actcattctt
     1441 tatggtaaaa gtaaatagtg atggaactgg tgagataatt tgatgtccca atttcttgag
     1501 catattccaa atggtattac tactgcctga agcgagtaaa atataatcag cattgataca
     1561 cttatcatta aaaaacactt tgtatgtatc ttgtttttcg atgtgtgtta ccttggcatg
     1621 cttgtttatt tcaattttta aaacatggca ttgctgtata aaacaatcga tgatagtttg
     1681 tgagcgatta gacacaggaa acatacgacc atcgctttct gtttttaatg cgacttgatg
     1741 atgattaaac caatcgaccg tatgttcaca attgaaagta taaaatggtc cgactaattc
     1801 ttttgaacca cgtggataaa atttcaccaa ttctttgaca tcaaaacatg catgtgtgac
     1861 attacaacgt ccaccgcctg atattttcac cttacctagt acttcattgc cctgctccaa
     1921 gataataata gccaaatcag gattgttttc agccgctgta atggcagcga aaaagcccgc
     1981 agcaccaccg ccaatgacca ctaatttttg ctttgaattc atttgtctgc aaatatcctt
     2041 ttttatcttc aaaatgtgga tatttccatg aaatatcaca taaaaataag gcttaaaaaa
     2101 ggtatctttg tcaagtaaaa aaatacgtaa atgtcagaag aattagaagg gttgaacaga
     2161 cctagaattg tccctattaa cattgaagat gaaatgaaat cggcttacat agattattct
     2221 atgtccgtta ttgtatcacg tgccttgcca gatgttcgcg atggtttgaa gccagtacac
     2281 agacgtgtgc tttttggtat gtctgaatta ggcatgacct ctaataaacc ttataaaaaa
     2341 tctgcacgta tcgtcggtga ggtgttaggt aaataccatc cacatggcga tgcctctgta
     2401 tatgatacca tggtgcgtat ggcacaagcg tggtctttgc gatacacttt gatagatggt
     2461 caaggtaact ttggttcaat agatggcgac cctccagcag cgatgcgtta tacggaagca
     2521 agattgaaaa agattgcgga agatatgata gaagatttgc acaaagacac agttgatttt
     2581 cagctcaatt tcgatgattc tttggaagaa ccaacagtaa tgcctacacg aattccacaa
     2641 ttactagtga atggtgcatc tggtatcgct gtaggtatgg ctacgaatat gttgcctcat
     2701 aatttatcag aagtaatcga tggtattgtt gcttatattg ataatagaga aattactata
     2761 gatgaattaa tgcaatttat taaagcacct gattttccaa caggtggtat tatctatggt
     2821 tatgaaggca taaaagatgc atttagaact ggacgtggta aagtgcgcgt tagaggaaaa
     2881 gccagaatcg aagaaaataa agaaggcaga acacaaatca ttatctcaga agtgccttat
     2941 caaatacagc caaagatgat tatcacgcgt gctgctgatt tggtgaatga gaaaaaattg
     3001 gaaggtatat ctgaaattag agatgagtca gacagagatg gtttacgcgt tgtttttgat
     3061 ttgaaaagag atgctattcc aaatgttgtc ttaaataatt tataccaatt aacgccactg
     3121 caaaccagct atggtgtaaa taatatagca ttggtgaatg gtcgtccaca attattgaat
     3181 cttaaagaaa tgattcaata ttatgtagat tttagacatg aagtggtgac gcgccgaaca
     3241 cagttcgaat tgcgaaaagc agaagaaaga ttacatattt tagaaggata tttaatagct
     3301 ttagacgctt tagatgaagt cattcagctc atcagaaaat ccaaaaatcc tgaagaagca
     3361 aaagatggct taatgaataa cttcggcatg tctgaattgc aggcaaaagc ggtattggca
     3421 ttaaccttac aacgattaac aggtttagaa atcgacaaga ttagagctga acataaagaa
     3481 gtttcagacc ttattgctaa gcttaaagaa atcttagcga atgaacattt gcgttttgaa
     3541 atcatcaaag aggaaatgtt ggaaataaag aaaaaatatg gtgatgaacg tcgttctgaa
     3601 atcgtgcttt atggtgatga tatttcgtat gaagatatga tagcagaaga agaaatggta
     3661 gttactattt ctcatttagg ctatgtaaaa cgtacttcat tagatgaata caaaaaacaa
     3721 aacagaggtg gcaaaggttc gcaaggtggc aaaacaagag gagaggactt tatagagcat
     3781 ctttttgtgg cttcgacaca caattattta ttgttgttca cagagcaagg taaatgtttc
     3841 tggttgaaag tttatgaaat tccggaaggc aataaaacgt cgaaaggtcg tccaattcag
     3901 aatgtcatta atattccaca agacgataaa atcaaagcat ttataaatgt cgaagattta
     3961 aaaaatgagg aatttttaga ttcacattat atcgtcatgg caacaaaacg tggtactgtc
     4021 aagaaaacct ctatgcgcga gtattctcgt cctcgttcaa atggcgttaa tgccatcaca
     4081 gttcgtgaag gtgatgaatt gataactgca gcattaacaa atggaaaatg cgaaattatc
     4141 ttaggctcgc aaaaaggata tgctgtgcgt tttaatgaag aagacgttcg ctctataggt
     4201 cgtactggct caggagttcg cggaatcacc ttacgtggcg acgatgatga actaattggc
     4261 atggtttgtg cagatacaag ttccgatata aaagaagata tcatggttgt ttctgaaaat
     4321 ggatatggca aacgctctgc actcgatgat tatcgcatta catcacgtgg tggaaaagga
     4381 gttatttcat taaacgtaac cgaaaaaaca ggcaatatga ttgccatcga atccgtgaca
     4441 gacaataacg atttaatgat tatcaataaa tctggtctca ccattcgaat tggattaaat
     4501 acattgcgca cgatgggcag aaacacacaa ggtgtgagct tgataaaatt gaaagatgat
     4561 gatgctattg ctgcagtatc taaaattgaa cacgaagaag aggaagaaaa tgaaaatgta
     4621 gaaaataatc atacagatac agaccagtca tcatcaaacg aagataattc taatcaacat
     4681 tcagaaatac aaaatgaaga ataatatgaa cacattaaaa ctgatagtac ttttagtaag
     4741 catcgcatta tatacgcaaa gtgctttcgc tcaaaaagga aaaataacat cagcacaatt
     4801 aagcttgcaa gatggaaaag tgttagatgc taagaaagat atcgacttag cattagccaa
     4861 tgaagaaatt caaaaacgtg tagatgcttg gacaacaaaa ggtgaggtat atacgcagat
     4921 ttatgtaaca aaattatatt atgcgcaaaa tccaaattgc ctttttgatg caaaagcagc
     4981 ttacttaaaa gcttttgaat tagaaactaa tccaaaaaag caaaagaatt tttcgacgcc
     5041 attaaatgat ttgtcaggct atctatttaa tgaagcattg aacagattta atgctaaaaa
     5101 gtatgatgat gcttacacac attttgatgc cagcagaaca attaatgaat ttttgttttc
     5161 taaaaattta gtgagcacaa tcgatacaaa tgctatttat gcaacggcaa tggctggcgc
     5221 aaatgcaaac aaaatcgctg aagtaaaacc attgttggaa aaattaataa caatgaatta
     5281 tgacaatcct gctgtttatg agaccttggc acaagtgtat gaaacagaaa aaaatacaga
     5341 tgctttgaaa aaattggtga gtgcaggttt gaaaaaatat cctaatagta agaacttaca
     5401 aatctatgat ttgaatgcag ccttagacaa tacagatgtg aatgaatcta tcacaaaatt
     5461 tgaagcggca ttggcaacag atcctaaaaa tgcttcaatt gcattcaatt taggtgtatt
     5521 gtatgacaaa gtgcaaaaca cagcgaaaac aaaagaatac taccaaaaag caatcgaatt
     5581 aaaacctgat tatggtgatg cttatttcaa cttaggtgta atgtatttca atgctggtgt
     5641 agttaaaaat aaagaaatga atgccgtcga tgatacaaaa gatagagatg gaaaaattta
     5701 tgaagctttg aagaaagaac gcaatgattt atttcaatta gcattgccta atttagaaaa
     5761 agcatatgca atcgatccta aaaatataga ctataaagcc aacttgaaaa aagtgtatgc
     5821 gagcatgaat atgttagata aagctaaagc tttagattaa cgcaatcaaa taattataaa
     5881 aaaaaggtcg aacgcagttc gacctttttt tatatagttg atacagaaaa ttacattaaa
     5941 agttacaata catatttacc atgtgatgtg taattttaca ttg
//
LOCUS       DAOQQV010000141         5920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5822_length_5920_cov_7.774425, whole genome shotgun sequence.
ACCESSION   DAOQQV010000141 DAOQQV010000000
VERSION     DAOQQV010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5822_length_5920_cov_7.774425"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLL59_11275"
     CDS             <1..193
                     /locus_tag="PLL59_11275"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HMY24433.1"
                     /translation="ILKGFLSKFSSRDFIVVHKGNAKDGAQYIIGSLKTNAGSYRTYI
                     FIKKTGTTNYIQEIRFEKE"
     gene            231..1082
                     /gene="nadC"
                     /locus_tag="PLL59_11280"
     CDS             231..1082
                     /gene="nadC"
                     /locus_tag="PLL59_11280"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HMY24434.1"
                     /translation="MLDQQELDIFISNALHEDVREGDHSSLASIDKDTQGKARLLVKD
                     QGILCGLEVANAIFNKVDAQLRMEIHINEGETVKYGDVAFHVYGNAISILTAERLVLN
                     CMQRMSGIATNTNEYVKAIAGTHAKVIDTRKTTPNIRFLEKYAVTIGGGFNHRMGLYD
                     MVMLKDNHIDFCGGISKAIQKVHHYLQAKQLDLKIEVETRDLDDVKEVLQYGGVNRIM
                     LDNFTPEKVREAVSLINGRFETEASGGITLNTIRSFAETGVDFISVGALTHSVRSLDL
                     SLKAEIH"
     gene            1114..2013
                     /locus_tag="PLL59_11285"
     CDS             1114..2013
                     /locus_tag="PLL59_11285"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HMY24435.1"
                     /translation="MKKKVRFIVNPKSGGTFGAKFSERLVRQHLDLSKFDYDIFVTKY
                     AGHAIKIAQQSLIEKIDIIAVAGGDGTQNEVGQMLLNSPIIMASIPTGSGNANARKLK
                     IPIDVAKSIQLINTGKAFKIDALILNGQPFFMAAGIGYDAKVVEQFDKLPFRGVGAYL
                     TGVLTTAFTYKLPHYTIEIDNEKTIETEAFTVLVTSTGQLGYNVEFTKNSILNDGKFE
                     IAIVKNFDRTRVPSLIYESFYGDIASQDFLETHTVNKILKIRTNSIESIQMDGDYKGK
                     TNYIEVTCKKQALNFLVDQDFVL"
     gene            2069..2410
                     /locus_tag="PLL59_11290"
     CDS             2069..2410
                     /locus_tag="PLL59_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HMY24436.1"
                     /translation="MAEDWKKKLGNSVVYSTNPDYKPASDEDEIETLPNSKQLLYISL
                     DKKQRKGKTVTLVDGFVGKNEDLEVLAKLLKTKCGVGGSAKDNEIIIQGDFKLKVKEI
                     LEKENYKVKVK"
     gene            2429..2926
                     /locus_tag="PLL59_11295"
     CDS             2429..2926
                     /locus_tag="PLL59_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMY24437.1"
                     /translation="MNFEHELSNLKQHAKQQRKLYIKTVQQLKKLKPYDVDELFHTQH
                     DDIFKVVDCLECANCCKTTPALISNEDINRIAKHLRISSQDFIKKYTRKDEDGDTVLN
                     KTPCIFLKDDNKCAIYEVRPFACKDYPHTNRKKMHQVLDLSIKNTEICPAVARILDNI
                     SESLA"
     gene            complement(2930..3439)
                     /locus_tag="PLL59_11300"
     CDS             complement(2930..3439)
                     /locus_tag="PLL59_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMY24438.1"
                     /translation="MQLLEAKKQFVQAWGALGSQWGINKTMAQIHALMMVSTEPLSTE
                     EIMEELSISRGNANMNIRELTDWGLVTKVFKAGERRDYFVGEKEVWKIAKQVAKIRKT
                     RELDPILKLLEQLKTVNAHIKNDELKHFQKMVNDVHKLAVNVDRLIESIFKAEESWFW
                     DKILKIFTK"
     gene            3821..>5920
                     /gene="dnaE"
                     /locus_tag="PLL59_11305"
     CDS             3821..>5920
                     /gene="dnaE"
                     /locus_tag="PLL59_11305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMY24439.1"
                     /translation="MCDFAPLHCHTQYSLLDGAADIDAMVRKAKADNMPAVAITDHGN
                     MFGAFKFFSACKKHDIKPILGCEVYVVEDRFNKKFTRDNKDKRYHQLLLAKNELGYKN
                     LMKIVSLGFIDGIYMDFPRVDRALIEQYSDGLIATTCCVGAEVPQTMITKGVEAAEKV
                     FLEWLKIFGDDYYIELQRHEIDDLDGTGWSQEAINKELIKFGKKHNVKIIATNDSHYV
                     DQKDSEAHDILLCLQTGKDFIDPNRFKFANNKFYFKTQFEMKETFKDVPYAIENTLEI
                     VEKVEKLNLQRDILLPAFQLPVGFNNQDDYLRFLTIEGAKKRWGSILSLDVVERLDFE
                     LKTIQHMGFPGYFLIVQDFINEGKKLGVSIGPGRGSAAGSAVAYAIGITNVDPIKYNL
                     LFERFLNPDRISMPDIDIDFDDEGRERVIDYVIDKYGKNQVAQIITYGTMAAKSSIKD
                     VGRVLKVPLSDTDKMSKLVPEKPKITLEKAYEEVEELRNMRDNPMYKQTLQLAEELEG
                     SIRNRGIHASAVIIAPDDIVNYVPVCTAKDAELLVTQFDGSIIESAGMLKMDFLGLKT
                     LTILRDALKLIEKNHDIKIDLDTIPLDDKKTFELYKSGDTIGTFQFESAGMQKYLRDL
                     QPDKFDDLIAMNALYRPGPMEYIPNFAARKNGREKVQYDLPEMEEYLKETYGITVYQE
                     QVMLLSQKLAGFTKGKAD"
BASE COUNT         2140 a          981 c         1061 g         1738 t
ORIGIN      
        1 tattctaaaa ggatttttat ctaaattctc ttcacgagat tttattgttg tacataaagg
       61 caatgccaaa gatggcgcac aatatataat tggttcttta aaaacaaatg ctggttcgta
      121 tagaacatat atttttatca aaaaaactgg aacaacaaat tatatacaag aaattcgatt
      181 tgaaaaagaa tagtcatctt tatatattta tttgacaaga aaaatctctc atgttagacc
      241 aacaagaact cgatatattt ataagcaatg ccttacacga agacgtgcgc gagggcgatc
      301 actcttcatt agcatcaata gacaaagaca cgcaaggtaa agcacgatta ctagtaaagg
      361 accaaggcat tttgtgtgga ttagaagtag caaatgcaat ttttaacaaa gtcgatgcgc
      421 aattgcgaat ggaaatacac atcaatgaag gtgaaactgt gaaatatggt gatgttgcat
      481 ttcatgtgta tggaaatgcg atttcaatat taacggcaga acgcttagta ctgaattgta
      541 tgcaacgcat gagtggcata gctaccaata ccaacgaata cgtaaaggcg atagcaggca
      601 cgcatgcaaa agtaattgac acaagaaaaa caacgcctaa cattcgtttt ttagaaaaat
      661 atgccgtcac cattggcggt ggcttcaacc acagaatggg cttgtatgat atggttatgc
      721 ttaaagacaa tcacatcgat ttctgtggcg gcatcagcaa ggccatacaa aaagtgcatc
      781 actatttaca agctaagcaa ctggatttaa aaatagaagt tgaaacgcgc gacctagatg
      841 atgtaaaaga agtgttgcaa tatggtggcg taaatcggat tatgctggat aattttacgc
      901 cagaaaaagt acgcgaagct gtctccttaa ttaatggtcg atttgagaca gaagccagtg
      961 gcggtattac tttaaatact attcgcagct ttgcagaaac aggcgtagat ttcatttctg
     1021 ttggagcatt aacacattct gttcgtagct tagatttgag tctaaaagct gaaatacact
     1081 agcatataaa atgcaatatt aaaatatatg agcttgaaaa aaaaagtacg atttatagtt
     1141 aatccaaaat ctggtggcac ttttggtgca aaattttctg aacgattagt acgtcaacat
     1201 ttagacttat cgaaatttga ttacgatatt tttgtaacaa aatatgctgg acatgcaata
     1261 aaaattgcgc agcaatcact catagaaaag atagatatta ttgctgtcgc tggtggtgat
     1321 ggcacacaaa atgaagttgg tcaaatgctt ttaaactcgc caattattat ggcgagcatt
     1381 ccgactggct cgggcaatgc caatgccaga aaacttaaaa taccaattga tgttgcaaag
     1441 tcaatccagc tcatcaacac aggaaaagca tttaaaatag atgcgctaat actgaatggc
     1501 caaccttttt ttatggcagc aggaattggc tatgatgcta aagtggtaga acaatttgac
     1561 aaattaccat tcagaggtgt aggtgcttat ttaacaggtg ttttgacgac cgcatttaca
     1621 tacaaacttc cacattacac catagaaatt gacaatgaaa aaacaataga aacagaagcg
     1681 tttacagtat tagtcacaag tactggacaa ttaggataca atgttgaatt tactaaaaac
     1741 agcatactaa acgacggaaa atttgaaata gcaatcgtca aaaattttga tagaactcgt
     1801 gtaccaagct taatttacga aagtttttat ggtgacatcg cttcgcaaga ttttttagaa
     1861 acacatactg tcaataaaat acttaaaata agaacgaata gcattgagag catacaaatg
     1921 gatggcgatt ataaaggaaa gacgaactac atagaagtta catgcaaaaa acaagcactt
     1981 aattttttag ttgaccaaga ttttgttttg taatgctatg tctttaatat tttaatgcta
     2041 tcattttttc acaacaaaat attatacaat ggcggaagat tggaaaaaaa aattgggaaa
     2101 tagtgttgtc tattcaacaa atccagatta caagcctgcc tctgatgaag atgaaataga
     2161 aactttgcct aacagcaagc aattgctata tatttcctta gacaaaaaac aacgcaaagg
     2221 caaaacggtg actttggtgg atggatttgt aggcaaaaat gaagacttag aagtgttagc
     2281 aaaacttctc aaaaccaagt gtggcgttgg tggctccgca aaagataatg aaattattat
     2341 tcaaggtgat tttaagctaa aagtaaaaga aattttagaa aaggaaaatt acaaggtaaa
     2401 agtaaaataa cgataattac attcaatcat gaatttcgaa catgaattat caaatttaaa
     2461 acaacacgca aaacaacaac gtaagttgta tatcaaaaca gtacaacaac taaaaaagtt
     2521 aaaaccttat gatgtagatg agctttttca cacacaacac gacgatattt ttaaagtagt
     2581 tgattgttta gaatgtgcta attgctgcaa aacaacacct gctttgatat ctaatgaaga
     2641 catcaataga atcgccaagc atttacgaat atcctcacaa gattttataa aaaaatatac
     2701 cagaaaagat gaagatggcg ataccgtttt aaacaaaacg ccatgtattt ttttgaaaga
     2761 cgataataaa tgcgctattt atgaagtgcg tccatttgct tgtaaagact atccacatac
     2821 gaatagaaaa aagatgcatc aagtattaga tttaagcatt aaaaacacag aaatttgccc
     2881 agctgtagca cgtattttag acaacattag cgaaagcctt gcttaaaaat tattttgtga
     2941 aaattttcaa gattttgtcc caaaaccaac tctcttctgc tttaaaaata ctttctatta
     3001 atctatctac gtttacagca agtttgtgaa catcgttgac cattttttgg aaatgtttga
     3061 gttcatcatt tttaatatgt gcatttactg tttttagctg ttccagtaat tttaaaatcg
     3121 ggtctaactc tctggttttt cgtattttag caacttgctt cgctattttc caaacctctt
     3181 tttcgcctac aaaataatct ctgcgctcac ctgctttaaa tacttttgtc accaaacccc
     3241 aatcggtcaa ttcgcgaata ttcatattgg catttccgcg tgaaattgat aactcttcca
     3301 tgatttcctc tgtactcagt ggctccgtag aaaccatcat caaggcatgt atttgtgcca
     3361 tcgttttatt gatgccccat tgagagccaa gggcacccca agcttgtaca aattgttttt
     3421 ttgcttcgag caattgcatt ttcaaatgta caaaagttaa ttatactttc aaaaaatact
     3481 gaaagcaata tcaaattctc aagcaaatga ccgaatacta agtcagaacg accgattact
     3541 cattctaatt gatttacatc aatacattag ctaaaattac aatacaaaaa caaaccataa
     3601 aacagaccat aaaactatga ttaacctaat ttttatcatc ctcaaataat tcttaataat
     3661 tcttagttgc gtgtagctcg gtgcaatgcc gagctttttt gtgtctaata atcaatgtat
     3721 tagattgttg aaaaataaaa aatggaatct aatacaatcc tgttcttttc tattgtaatt
     3781 tcactaacca atccatcaca aatttttaaa ttcaagtatc atgtgcgatt ttgctccact
     3841 ccattgtcat acacaatatt ctcttttaga cggcgctgcc gacattgacg caatggtgcg
     3901 caaggcaaaa gctgataata tgcctgctgt tgcgattaca gaccatggca atatgtttgg
     3961 tgcatttaag tttttttcag cttgtaaaaa gcacgacata aaacctatat taggctgtga
     4021 agtttatgtt gttgaagatc gctttaataa aaaatttaca agagacaata aagacaaacg
     4081 ctaccatcag cttttattag ctaaaaatga acttggttat aaaaatctaa tgaaaatagt
     4141 ttctctaggt tttattgatg gtatatatat ggattttccg cgtgtagaca gagccttgat
     4201 tgagcaatat agcgatggac ttatagccac tacatgttgc gtaggtgctg aagtgccaca
     4261 aaccatgatt acgaaaggtg tggaagctgc cgaaaaagta tttttagagt ggttgaaaat
     4321 atttggggac gactattaca tcgaattaca aagacacgaa atagatgatt tagatggaac
     4381 aggctggagt caagaagcga ttaacaaaga actgataaaa tttggcaaaa agcacaatgt
     4441 aaaaataata gcaaccaatg attcgcatta tgtcgaccag aaagatagcg aagcacatga
     4501 tatcttatta tgcttgcaaa caggcaaaga ttttatcgat ccgaatcgtt tcaaatttgc
     4561 gaataataaa ttttatttca agacacaatt tgaaatgaaa gaaactttta aagatgttcc
     4621 atacgccatc gaaaatactt tagaaattgt agaaaaagta gaaaaattaa acctacaacg
     4681 cgatatttta ttgcctgcat ttcaattacc agtaggtttt aataatcaag atgattatct
     4741 gagatttttg actattgaag gtgcaaaaaa acgatggggc agcattttat ctttagatgt
     4801 cgtggaaaga ttagattttg agttaaaaac catacagcac atgggttttc caggctattt
     4861 cttaatcgtt caagatttta ttaatgaagg gaaaaaactc ggtgtatcaa ttggtccagg
     4921 tcgcggttct gctgcaggtt ctgctgtagc ttatgccatt ggcatcacca atgttgaccc
     4981 aataaaatac aatttactct tcgagcgttt tttaaatcct gaccgtattt cgatgccaga
     5041 tattgatatc gattttgacg atgaaggcag agaacgagtt atcgattatg tgattgacaa
     5101 atatgggaaa aatcaagtag cacaaattat tacttatggc accatggctg caaaatcttc
     5161 gataaaagat gttggtcgtg ttttaaaagt gccattatct gacacggata aaatgtcgaa
     5221 attggtacca gaaaaaccaa aaatcacatt agaaaaagcc tatgaagaag tggaggaatt
     5281 gcgaaatatg cgcgacaatc caatgtataa acaaacttta caattggcag aagaactgga
     5341 aggctcaata cgaaacagag gcatacatgc atctgccgta attatagcac cagacgatat
     5401 tgttaattat gtacctgttt gcacagctaa agatgccgaa ttattagtca cacaatttga
     5461 cggaagcatt atcgaatcag caggcatgtt gaagatggac tttttaggtt taaaaacctt
     5521 gacgattctt cgcgatgcat taaagctcat cgaaaaaaat cacgacataa aaatcgactt
     5581 agatactatt ccactcgacg ataaaaaaac atttgaactc tataaaagtg gcgacacaat
     5641 tggtactttc cagtttgaaa gtgctggcat gcaaaaatat ttacgcgact tacaacctga
     5701 taaatttgat gatttaattg caatgaatgc tttgtacaga ccaggaccaa tggaatatat
     5761 tccaaacttt gcggcgcgta aaaatggaag agaaaaggtg caatatgact taccagaaat
     5821 ggaagaatat ttgaaagaaa cgtatggcat caccgtgtat caggaacagg tgatgttgct
     5881 atcacagaaa ttagctggtt ttacaaaagg gaaagcagac
//
LOCUS       DAOQQV010000142         5906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5845_length_5906_cov_9.750641, whole genome shotgun sequence.
ACCESSION   DAOQQV010000142 DAOQQV010000000
VERSION     DAOQQV010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5845_length_5906_cov_9.750641"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1224
                     /locus_tag="PLL59_11310"
     CDS             202..1224
                     /locus_tag="PLL59_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMY24440.1"
                     /translation="MGLFSFLNKEIAIDLGTANTLIIYKDQVVVDEPSIVAIDKKTNQ
                     VIAVGQKAMLMHEKTHANIETIRPLKDGVIADFTAAEAMIRGFIAMITDGNRWFRPSL
                     TMIICIPSGVTEVEKRAVIESAEHANARERYLIHEPMAGALGIGLDVELPEGNMIIDI
                     GGGTTDVAVIALSGIVTDQSIRVAGDDFTLDIVEYIKRQHNILIGERTAEQVKIHVGS
                     ALQDLESPPEDFAVHGRDLLTGIPKQVTVAYSEIAGCLDKSISKIEEAIMKALESTPP
                     ELASDIYRKGIYLTGGGALLRGLDKRIAMKTKLPVKVADDPLRAVVRGTGIALKNRHR
                     YTFLMS"
     gene            1229..2053
                     /gene="mreC"
                     /locus_tag="PLL59_11315"
     CDS             1229..2053
                     /gene="mreC"
                     /locus_tag="PLL59_11315"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HMY24441.1"
                     /translation="MRSIYYLFTRFYVLIFFVILEAFALSLIFKSNTYQEVKFLNTSG
                     NITGTFVNYLNSIKHFIHLGSTNSILLEENTRLKNQLYFQNKYPSDSSLPKNSTAYKF
                     EYIPAKIVNNTINKSINYITIDKGSKDGVEKGLGVISSNGVVGIITNVSTNYSLLMSV
                     ISVKTLIGVRHKKTNALGNLRWNAENPFILQVDGFSKTLPIKKNDTIVTAGFSSIFPP
                     DIPVAFVKNIEADESTSFFNLDVKLSNNISSLSYVYIVKNEKKKELDSLQTLIANE"
     gene            2046..2555
                     /locus_tag="PLL59_11320"
     CDS             2046..2555
                     /locus_tag="PLL59_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24442.1"
                     /translation="MNSKYFITNAIRFVLLIALQILLLNELPFGRAYLMVYPLAILLL
                     PVFTPRITVLFLGFFTGLTIDIFSNGGGLHAASLTAIAYARSYILDTFQPRSGWDKLD
                     VPNLSSQGYVWFFYYSLILFSIHHFLYFFLEVFSFANFFSTIFKTIISLFGSLVVVWM
                     ISLFFKRSK"
     gene            2573..4591
                     /gene="mrdA"
                     /locus_tag="PLL59_11325"
     CDS             2573..4591
                     /gene="mrdA"
                     /locus_tag="PLL59_11325"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMY24443.1"
                     /translation="MQRGNESGILEKAIYFAAIVLVVKLFFLQVLTPEYKLKAHDNVV
                     KKVTVYPSRGLIIDRNGKVMVYNGAVYDILVQWNITKNLDTLRLCELLNTDVDFVRKK
                     LDEIKRTTPNKPTPFIKLLDPQTFARFQEHLFEFPSFTFQVRTVRRYPYHSGAAVFGY
                     LSEVTENNIQQSGGYYELGDYMGTTGIEQYYDSLLRGEKGYTFQVVDVKNTYKGKYKN
                     GKEDKIPIAGWDMYAGLDAELQEYGEKLMKGKVGGIIAIEPATGEVLAYVSSPGYDPN
                     LLTGRYRRYNFNVLFKNYYKPLLNRPIQAMYPPGSTFKAAAALVTLQSHTHEEKWHWF
                     CPGGYRVGSHTVNCHGSHGIPDVQTALQYSCNSYFCKVYNDFIMDSIYKTPAIGYQKW
                     WDMMSVFGYGKKLGIDLKGEKRGNLPSVKYYNKIFGEGKWRAATVISNSIGQGEVLAT
                     PLQIANAMAIIANKGYYITPKISRYFVKDKKIRKPKTQKIQTGIDTVYYRYVTDGLEK
                     VVQAGTARIAQIPGISFCGKTGTAQNPHGKDHSIFSGFAPKDNPKIAIAVFVENAGFG
                     ATYAAPIASLMVEKYLNDTIAKKRIPLQERMFKARIINEEMEVGVPKTPEQIRQDSLA
                     KEKIRKDRIKFVEDSIKTAKEKDKNKTTPKTKKDSVLFFKKEDDIYQE"
     gene            4593..5816
                     /gene="rodA"
                     /locus_tag="PLL59_11330"
     CDS             4593..5816
                     /gene="rodA"
                     /locus_tag="PLL59_11330"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HMY24444.1"
                     /translation="MQANKRAYDPIDSTVLYCYLFLVILGIMSIFATEYNGVLGDTFL
                     SFKHSYIRQAIFAIASGLMFFTILGFDRRILQLISYPMYGLILLLLLSVFVFGERTKG
                     DQNWINLGFFKLQPSEFAKFATAMALAKYFDTPSMRFVTRKEQLTAYAIIGLPLLMVM
                     AQGDAGSSLVFAAFIFVLNREGLSDLYIYLGLYVIAVSVLSLIINQYIIIGISVVLFL
                     GLIYWNWMNKQLVKILTIVMIGTCIYTSSVSYIFNNVLEKHQRDRINVILGKELTKEA
                     KRGVAYNLNQSKIAIGSGGIIGKGYLQGTQTRYNYVPETSTDFIFSAIGEEWGFLGAV
                     VLIFVYTTMLLRLLWLAERQRSPYGRVYIYSVVAIIFTHVVINIGMTIGLLPVIGIPL
                     PLLSYGGSSLLSFPQ"
BASE COUNT         1975 a          999 c         1089 g         1843 t
ORIGIN      
        1 ttaacaagga taaacctatt ttaaacaaaa ttgcatttat ctcttgaatt cagcagcaaa
       61 tagattagtc cttatgtatt tgtaaaatca aggaatttgc tgattagtta ttaaaaatta
      121 aattttcgat tcacgattat tatatcattt ataaatctta cttttgttca ttaattagaa
      181 caatagttta agcgtagaaa aatgggatta tttagcttcc tgaacaaaga aatagcgatt
      241 gaccttggaa cggcaaacac actaatcatt tacaaagatc aagtagtggt agatgagccc
      301 tccattgttg ccatagacaa aaagactaat caagtcattg cagttggaca aaaagcaatg
      361 ttgatgcatg aaaaaacaca tgcaaatatt gagaccatca gaccattgaa ggacggagta
      421 attgccgatt ttacagcagc agaagcgatg atacgtggtt ttattgccat gattactgat
      481 ggaaacagat ggtttagacc atcattaacc atgattattt gtattccatc aggtgtaaca
      541 gaagtagaaa agcgtgctgt aattgaaagt gcggaacacg cgaatgcacg agaacgctat
      601 ttgatacacg agccaatggc tggcgcactt ggtattggtt tagacgtaga gcttcctgaa
      661 ggaaatatga ttattgatat tggtggcgga acaactgatg ttgctgttat cgcattatct
      721 ggtattgtca cagatcaatc tatacgtgta gctggcgacg actttacatt agacattgtt
      781 gaatatatta aacgccagca caacatatta attggtgagc gtactgcgga acaagtaaaa
      841 attcatgtag gctccgcttt gcaagattta gaatcaccac cagaggactt tgcagtacat
      901 ggccgagatt tattaacagg cattcctaaa caagttacag tggcttactc tgaaattgca
      961 ggttgtttgg ataagtctat ttcaaaaata gaagaagcca ttatgaaagc cttagagtca
     1021 acaccaccag aattagcatc tgacatttat cgcaaaggaa tttacctgac aggtggcggt
     1081 gcattattgc gtggtttaga caaacgaata gctatgaaaa caaaattacc ggtaaaagtt
     1141 gcagacgatc cacttcgtgc agtagtgcgt ggtactggca tcgcattaaa aaacagacac
     1201 cgttatacgt tcctgatgag ttaaacaaat gcgtagcata tactatttat ttactcgatt
     1261 ttatgtgctc atattttttg tgatattaga agcgtttgcg ctctcgctta ttttcaaaag
     1321 caatacgtat caagaagtaa aattcctcaa tacatcagga aatatcacag gaacatttgt
     1381 aaactattta aatagcatta agcattttat tcacttaggt tccaccaaca gcatactgct
     1441 tgaagaaaat actcgtttaa aaaaccaatt atattttcag aataaatatc ccagcgattc
     1501 ttcattacca aaaaattcta cagcctacaa attcgagtat attcctgcta aaatagtaaa
     1561 taacactatt aacaaaagta tcaactacat tacaatagac aaaggaagca aagatggtgt
     1621 ggaaaaaggt ttaggcgtaa ttagtagtaa tggtgttgtt ggaattatca ctaatgtttc
     1681 gaccaattat tctttattga tgtcggttat aagtgttaaa acattaatag gcgttcgaca
     1741 caaaaagaca aatgcacttg gcaacctaag gtggaatgct gaaaatccat ttatactaca
     1801 agtagatggc tttagtaaaa cattgcctat taaaaagaac gataccattg ttactgcagg
     1861 tttctcttct atttttccgc cagatatacc tgttgctttt gttaaaaata tagaagcaga
     1921 tgagagtaca agtttcttta atttagatgt caaattaagc aacaacatca gctccttgag
     1981 ttatgtatat attgtaaaaa acgaaaagaa aaaagaatta gattctcttc aaactttaat
     2041 agcaaatgaa tagcaaatac ttcatcacta atgccattcg ttttgtattg cttattgcat
     2101 tacaaatatt gctattaaat gaacttccat ttgggagagc ttatctaatg gtatatcctt
     2161 tggctatttt acttttgccg gtctttacac cacgtatcac ggttttattc ttaggttttt
     2221 ttacaggact aactatcgat atatttagta atggcggtgg tttacatgct gcttcgctca
     2281 cagcaattgc ctatgcacgc agctatattc tcgatacttt tcaaccacgc tcaggctggg
     2341 acaaattaga tgttcccaat ctatcaagtc aaggttatgt ttggttcttc tattattcat
     2401 taatattatt ttctattcat cattttctat atttcttttt agaagttttc tcgtttgcta
     2461 attttttctc cactatattt aagacaatta tcagtctatt tggctctctt gttgtagttt
     2521 ggatgataag tctatttttt aagcgcagta aataaaacga gaacaccatt ttatgcaacg
     2581 aggaaatgaa agcggcattt tagaaaaagc aatctatttt gcagccatcg tgcttgttgt
     2641 caaactattt tttctacaag ttctcacgcc agaatacaaa ttaaaagcgc acgacaatgt
     2701 ggtgaaaaaa gtaacagtct atccttcgcg cggattaatt atcgatagga atggaaaagt
     2761 aatggtgtac aatggcgccg tttatgatat tttggtgcag tggaatatca caaaaaactt
     2821 agacacactc agactttgtg agttattaaa taccgatgta gattttgtcc gaaaaaaatt
     2881 agatgagata aaacgtacta caccaaataa accaacacct ttcattaagc tattagatcc
     2941 tcaaaccttc gcacgttttc aagagcattt atttgaattt ccatctttta catttcaagt
     3001 aagaacagtg cgccgatatc catatcacag tggtgctgct gtatttggat atttaagtga
     3061 ggtgaccgaa aataatattc aacaatctgg tggttattat gaattaggtg actatatggg
     3121 aacaacagga atagagcaat attatgacag tttattgcgt ggcgaaaaag gatatacttt
     3181 ccaagtcgta gatgtaaaaa atacatacaa aggaaaatat aaaaatggta aagaagataa
     3241 aatacctatt gctggctggg acatgtacgc tggtcttgat gctgaacttc aagaatatgg
     3301 tgaaaaatta atgaaaggaa aagtaggagg cataattgct atcgaaccag cgactggtga
     3361 agtattggca tacgttagca gtcctggtta tgatccaaat ttattgacag gcagatatcg
     3421 acgttataat tttaatgtgt tatttaaaaa ctactataaa ccattgttaa accgaccaat
     3481 ccaagcgatg tatccacctg gctctacatt taaagccgct gccgctttgg tgacattgca
     3541 aagtcataca catgaagaga aatggcattg gttttgtcct ggtggctatc gtgttggcag
     3601 ccacacggta aattgtcacg gctcgcatgg tattcctgat gtgcagactg ctttacaata
     3661 ttcttgcaat tcctatttct gtaaagtcta taatgatttc attatggatt cgatttataa
     3721 aacgccagcc attggctatc aaaaatggtg ggacatgatg agtgtttttg gatatgggaa
     3781 aaaattagga attgatttaa aaggagaaaa aagaggcaac ctgccaagtg ttaaatatta
     3841 taataaaata tttggtgaag gcaaatggcg tgctgcaact gttatttcta attctattgg
     3901 gcaaggtgaa gtgttggcaa caccattgca aattgctaat gcgatggcga ttattgccaa
     3961 caaaggatat tatattacac caaaaataag tcgctacttt gtaaaagata aaaagataag
     4021 aaaaccaaaa acacaaaaaa ttcaaacagg catcgatacc gtatattata gatatgtaac
     4081 tgatggactt gaaaaagtag tgcaagctgg taccgcaaga attgcacaga ttcctggtat
     4141 tagcttttgc ggaaaaacag gaacagcaca aaatccacat ggaaaagatc actccatatt
     4201 ttctggcttt gcaccaaaag acaatcctaa aattgcaatt gccgtattcg tagaaaatgc
     4261 tggatttggc gctacttatg ctgcacctat tgcgtcgcta atggtagaaa aatatttgaa
     4321 tgacaccatc gcaaaaaaaa gaataccact gcaagagcgt atgtttaaag ccagaatcat
     4381 taatgaagaa atggaagttg gcgtacctaa aacgccagaa caaatcaggc aagactcctt
     4441 agcaaaagag aaaataagaa aagacagaat taaatttgta gaagactcta tcaaaacggc
     4501 aaaagaaaaa gataaaaata aaacgacacc taaaacaaaa aaagatagcg tactattttt
     4561 taaaaaagaa gacgatatct atcaagaata acatgcaagc aaataaacga gcatacgatc
     4621 cgattgacag tactgttttg tattgttatt tattcttagt catattaggc atcatgtcta
     4681 tttttgccac agaatataat ggtgtgcttg gcgatacttt tctcagtttc aaacacagct
     4741 atattcgtca agccattttt gccattgcct cagggttgat gttttttacc atcttaggat
     4801 ttgacagaag gatattgcag ctcatctcct atccaatgta tggccttatt ttattactgc
     4861 tcttatctgt ttttgttttt ggcgagcgaa cgaaaggcga ccaaaactgg attaatctcg
     4921 gatttttcaa gcttcaacca tcagagtttg cgaagtttgc aacggcaatg gcacttgcta
     4981 aatatttcga cacaccatca atgcgctttg tgacacgcaa agaacaactc acagcctatg
     5041 ctatcattgg attgccttta ttaatggtaa tggcgcaagg cgatgctggc tccagcttag
     5101 tatttgctgc ttttattttt gtgctgaaca gagaaggttt atctgattta tatatctact
     5161 taggattgta tgtcattgcc gtgtctgtcc tttcgcttat catcaatcaa tacattatta
     5221 tcggcattag cgtagtcttg tttttaggtt taatttattg gaattggatg aataagcaac
     5281 tcgtaaaaat tttaaccatt gtgatgatag gtacttgcat atacacatca tctgtaagtt
     5341 atatttttaa taatgtatta gaaaaacatc aacgcgatag aatcaatgta atattaggta
     5401 aagaattgac aaaagaagcg aaacgtggcg ttgcctataa tctcaatcaa tccaagattg
     5461 ccattggctc aggtggcatc attggaaaag gctatttgca aggcacacaa acaagatata
     5521 attatgtacc agaaaccagc actgacttta tcttttcagc aattggtgaa gagtggggat
     5581 ttttaggcgc agtcgtattg atttttgtgt acactaccat gctattacgg cttttgtggc
     5641 tcgccgaacg gcaacgaagt ccatacggta gagtatatat ctattctgtt gttgctatca
     5701 tatttacgca cgtcgtcatc aacataggta tgactattgg tttattacct gttattggta
     5761 ttccattgcc attgctaagt tatggcggtt catccttatt atcttttcct caataaaatt
     5821 aactttgata tttttattat tattacaact gttagccctc acatccttta cactactacg
     5881 tgatactagt ttttagaaaa taaata
//
LOCUS       DAOQQV010000143         5888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5877_length_5888_cov_7.304303, whole genome shotgun sequence.
ACCESSION   DAOQQV010000143 DAOQQV010000000
VERSION     DAOQQV010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5877_length_5888_cov_7.304303"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1861)
                     /locus_tag="PLL59_11335"
     CDS             complement(215..1861)
                     /locus_tag="PLL59_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HMY24445.1"
                     /translation="METDKATNFIEEIIDADLKAGKYKSIITRFPPEPNGYLHMGHAA
                     SICLNFGVAQKYGGTTNLRFDDTNPVTEDTEYVDSIMADVKWLGFNWANVFYASDYFE
                     QLYAYAVQLIQQGDAYVCDLTADEIAAQKGTPTEAGTHSPYRNRSVAENLALFEEMRD
                     GKYKEGEKTLRAKIDMSSSNMLMRDPLLYRIKFAHHHRTGDKWCIYPMYDFAHGQSDS
                     IEKITHSICTLEFIPHREVYDWLIEKLGIYPSKQYEFARVNVNYTVMSKRKLLQLVNE
                     NHVTGWDDPRMPTISGMRRRGITANAIRTFCEKAGIAKREKVNEFSLLEFCVREELNK
                     IALRRMVVFQPLKVVITNLDKEIELNIENNPEDENGGTRQLMFTNEIYIEQEDFVEVA
                     QKKIFKMEVGQAIRLKGAFIVQCNEVIRNADHSIAEIHCTYYANSQSGQDTSGVKVQG
                     TLHWVSSKYAIPVEIREYDRLFKVENPADEEGDFKDYINENSLNIVNTALAEPSLKEA
                     TLTHHFQFLRKGYFYLDKESNPSTIIFNKTVGLKDNWSKK"
     gene            complement(1893..3395)
                     /gene="gltX"
                     /locus_tag="PLL59_11340"
     CDS             complement(1893..3395)
                     /gene="gltX"
                     /locus_tag="PLL59_11340"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMY24446.1"
                     /translation="MSVRVRFAPSPTGPLHIGGVRTALYNYLYAKKLGGDFILRIEDT
                     DQNRLVPGAEAYIIEALKWVGIEPNEGVGFGDGKYAPYRQSDRKESYRQYAEQLIQND
                     HAYYAFDTESDLEALKAKYPNFKYDASTRLEGKNSITLSAAEVAQRLANNDPYVIRIK
                     LPEEEHVHFEDEIRGNVMVSTTQMDDKVLLKSDGMPTYHLANVCDDYIMKITHVIRGE
                     EWLPSAPLHVLLYRYLGWENAMPKFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLEWT
                     DPVTKEISSGYKQGGYLPNAVLNMLAFLGWNPGTAQEIFTKEELVNAFSLDHIHKAGA
                     KFDFEKAKWFNAEYIKNLSNEQLAVLVKPIVTQKFGEQEEHYLIKAVGLVKERLVFLN
                     DIEHFAYLFTSIAQYDTTALSKITAIQQQFPKQAFEIFIQEQAFTDASSLEHAIKDFA
                     TANALKIGDVMKFLRTSLVGELSGPVISDLMILLGKDEVLKRVQNCLQQQ"
     gene            3552..4139
                     /locus_tag="PLL59_11345"
     CDS             3552..4139
                     /locus_tag="PLL59_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMY24447.1"
                     /translation="MLFQKVIWEFPASQQAIYLTFDDGPHQVITPWILSQLKRHDAKA
                     TFFCLGENAKKYPNLILEMERDGHAIASHGDAHLNGWKISKTEYEQNILQGKQTLSAI
                     LQKDIPLFRPPYGRFKHKVMPTQQIILWSLMPGDFDSSITAEKCLSRLKSVKAGDIIV
                     LHENEKSWKNLQYCLPKFLEFCKANNFQLSAIKNV"
     gene            4168..4941
                     /locus_tag="PLL59_11350"
     CDS             4168..4941
                     /locus_tag="PLL59_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMY24448.1"
                     /translation="MYLIDTHTHLYEEQFDDDRADMIQRALENGVSKMIIPNVDSTTI
                     QPMLDIVQQFPNAVFPMIGLHPCYVKPATYQQELAIIRNYLHSNEHQFVAVGEIGIDL
                     YWDKSTLDIQKEAFEIQCDFALEKNIAIAIHSRDATHILIDILKKRKRNPKGVFHCFT
                     GSVEEAKEILKLGFFLGIGGVVTYKNTHLRDTLLQLPLDRIVLETDAPYLPPVPYRGK
                     RNESAYTKIVAETLCSVYQKEFEEIASVTTQNATALFGI"
     gene            4967..>5888
                     /locus_tag="PLL59_11355"
     CDS             4967..>5888
                     /locus_tag="PLL59_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24449.1"
                     /translation="MKKVLFLFSTISIFLFSCTKPQDVTGPKLIFKFKFDSTQARLNN
                     FGQTVTVLPVGHSAMSPKFHLMSSHYIELAPDSLSPIGTGDILYRAAEVGSGDDLAID
                     FSKSVKAGNNEVFFSVPLKNITPGAFKWLRVSLVYQNYDIKIKYVNDALPIANGYYDG
                     NVASFVGFNTYLTKYTVKTQQVTVNAAKKQGYGAVELFNLPSGFPPQAPIEFQAPPNA
                     TTVPNPISNTSPIPAGSCVVTGRFAPISGFVITGNETNDIVITVSLSTNKSFEWKDKN
                     GDGFYQPLNGANNTIVEDSVVDMGIRGLIPI"
BASE COUNT         1802 a         1082 c          999 g         2005 t
ORIGIN      
        1 atttagagaa aataatgaac gccatttata ccgtagtaga gaagtaataa atagagatat
       61 ttttaaaata taattgcgaa taaaaagagg aacaattttg tttctctttt tttattgata
      121 ctttattgtg tttgacgaaa ttctaaacat agtgtatctg tataaatgat agtaataatt
      181 aaaaatgctt gctgtgtcca gaggcaatac acttttattt tttgctccaa ttatctttta
      241 atccaacagt tttattaaaa ataattgtcg atggatttga ttccttatct aaataaaaat
      301 aacctttgcg cagaaactga aaatgatgtg ttaaagtagc ttcttttaat gaaggctccg
      361 ccaatgctgt attgacaata ttcaaactat tttcattgat ataatcctta aaatcacctt
      421 cttcatcggc aggattttcc actttaaata atctatcgta ctctcgaatt tctactggaa
      481 ttgcatattt tgagcttacc caatgtaagg tgccttgtac ttttacgcct gacgtatcct
      541 gtccactttg gctgttagca tagtatgtac aatgaatttc agctattgaa tgatctgcat
      601 ttctaatcac ttcattacat tgcacaataa aagcaccttt tagacgaata gcttgtccaa
      661 cctccatttt gaaaattttc ttttgcgcta cttcaacaaa atcttcttgt tcaatataaa
      721 tttcattcgt gaacattagt tggcgagttc caccattttc atcttctgga ttgttctcta
      781 tgtttagctc aatctctttg tctaaatttg tgataacaac ttttaaaggt tggaaaacca
      841 ccattctgcg gagtgcaatt ttattgagtt cctcgcgcac acaaaattct aataaagaaa
      901 actcatttac tttttctcgc ttggcgatgc ccgctttttc gcaaaaagtc cttatggcat
      961 ttgcagtaat accgcgtctg cgcattcctg aaatggtagg cattcttggg tcgtcccagc
     1021 ctgtaacatg gttttcattt accaattgca agagcttgcg tttgctcatt actgtgtagt
     1081 ttacgtttac tcgtgcaaat tcatattgct ttgatggata aatgcctaat ttttctatca
     1141 accaatcata tacttctcta tgcggaataa attctaatgt acaaatggaa tgcgttattt
     1201 tttcaataga atcgctttgc ccatgtgcaa aatcatacat cggataaata caccatttgt
     1261 cgccagtgcg atgatggtgt gcaaatttaa ttctgtataa taatggatca cgcatcaaca
     1321 tgtttgatga actcatatca attttcgcac gtaatgtttt ctcaccttct ttgtattttc
     1381 catcgcgcat ttcttcaaaa agtgcgagat tttctgctac acttctgttg cgatagggcg
     1441 agtgcgttcc agcctctgta ggtgtgcctt tttgtgctgc aatttcatct gctgttaaat
     1501 cacaaacgta tgcatcgcct tgttgtatga gttgtacagc atacgcatat aattgctcaa
     1561 aataatcgct cgcataaaat acatttgccc aattaaaacc taaccatttt acgtctgcca
     1621 ttatgctatc tacatactct gtgtcttctg ttacaggatt ggtatcgtca aagcgcaaat
     1681 ttgtagtgcc tccatatttt tgtgctacac caaaatttaa acaaatagaa gcagcatgac
     1741 ccatgtgtaa atagccattg ggttcaggcg gaaaacgtgt gataatactt ttatattttc
     1801 ctgcctttaa gtcagcatct attatttctt ctataaaatt tgttgcttta tcagtttcca
     1861 ttatttgttt gtttagatgt gtactaatct gtttattgct gttgtaaaca attttgtaca
     1921 cgttttaaca cttcgtcttt tccaagtaat atcattaaat cagaaataac agggccagaa
     1981 agttcgccga ccaaactggt acgtaagaat ttcattacat ctccaatctt taatgcattc
     2041 gccgtcgcaa aatctttgat agcatgctcc aatgaagatg cgtctgtaaa tgcttgttct
     2101 tgaataaata tttcaaatgc ttgctttgga aattgttgct gtattgctgt aatttttgat
     2161 aacgctgtag tatcatactg agctatgctt gtaaataaat aagcaaaatg ttcgatgtca
     2221 tttaaaaaca ccaaacgctc ttttactaag cctactgctt ttattaagta atgttcctct
     2281 tgttcaccaa atttttgagt gacgattggt tttactaaaa ccgccaattg ttcattgctt
     2341 aaatttttga tgtattccgc attaaaccat ttagcttttt caaaatcaaa ttttgcacct
     2401 gctttgtgga tgtggtctaa gctaaaagca tttaccaatt cttcttttgt aaatatttct
     2461 tgtgcagtgc caggattcca gcctaaaaat gccaacatat tcaatactgc atttggcaaa
     2521 taaccacctt gtttatagcc actgctaatt tcttttgtta ctgggtctgt ccactctaaa
     2581 ggaaatacag gaaatccgag acgatcgcca tcgcgcttgc taagtttacc attgccatct
     2641 ggttttaaca acagaggaag atgtgcaaat tttggcatcg cattttccca gcctaagtag
     2701 cgataaagca atacgtgtaa tggtgcactt ggcagccatt cttcaccgcg aataacatgt
     2761 gtaattttca tgatatagtc atcacaaaca tttgctaaat gataagtcgg catgccatcg
     2821 cttttcagta aaactttgtc gtccatttgt gtggtgctga ccataacatt acctctgatt
     2881 tcatcttcaa aatgtacgtg ttcctcttct ggcaatttta tacgaatgac atatggatca
     2941 ttattggcta atcgctgtgc aacttctgct gccgataatg tgatggagtt ttttccttct
     3001 aaacgcgtgg acgcatcata tttaaaattt ggatatttcg ctttcaatgc ttccaaatcg
     3061 ctttctgtat cgaatgcata ataggcatga tcattttgga ttaactgttc agcatattgt
     3121 cgatagcttt ctttacggtc gctttgacgg tatggtgcat attttccatc gccaaaacct
     3181 acaccttcgt ttggttctat gcctacccat tttagtgctt ctataatgta ggcttccgca
     3241 cctggcacca atctattttg gtcggtatct tcgatgcgta aaataaaatc gccacccaat
     3301 tttttggcat ataaataatt atacaatgca gtgcgcacgc cgccaatatg taatggaccg
     3361 gtcgggcttg gtgcaaatcg aactcttaca gacataattt agttttgtat caatttgaaa
     3421 atgctatatt tggctgtaaa attagatatt tatattttct tataaaaaca agtaaagtca
     3481 ttttataatt accacatctt caaattttta atcatttcat gtatttcgtt cgcacgccat
     3541 atatattaaa aatgcttttt caaaaagtga tatgggaatt tccggcatcg cagcaagcga
     3601 tttatctaac attcgacgat ggtccacatc aagtaataac accttggata ttgtcacaat
     3661 taaagcgaca tgatgctaaa gcgacattct tttgtttagg tgaaaatgcg aaaaaatatc
     3721 caaacttgat tttggaaatg gaaagagacg gacatgcgat tgctagtcac ggcgatgcac
     3781 atcttaatgg ctggaaaatt tcgaagactg aatatgaaca aaacattttg caaggaaaac
     3841 aaactttgtc cgctattctc caaaaagata ttccactatt tagaccacct tatggcagat
     3901 ttaaacacaa agtgatgcca actcaacaaa ttattttatg gtcattaatg cccggcgatt
     3961 ttgacagttc cattacagca gaaaaatgct tatcgcgttt aaaatcagtc aaagcaggcg
     4021 atattattgt attgcatgaa aacgaaaaat cttggaaaaa tttacaatat tgtttgccta
     4081 aatttttaga attttgcaaa gcaaataatt ttcaattatc agcaataaaa aacgtttaat
     4141 ttttttacac accattcatt acaagaaatg taccttatcg acacacatac ccatttatat
     4201 gaagaacagt ttgatgacga ccgtgctgat atgatacagc gtgctttgga aaatggagtt
     4261 tccaaaatga ttatacccaa tgttgattcc acaacaatcc aaccgatgtt ggatattgta
     4321 caacaattcc cgaatgctgt atttccgatg ataggcctac atccttgcta tgtaaagcca
     4381 gcaacttatc agcaagagtt agcaattata cgcaattatt tgcatagcaa tgaacaccaa
     4441 tttgttgctg ttggtgagat cggcattgat ttatattggg acaaatcaac attagatatt
     4501 caaaaagaag cttttgaaat acaatgcgat tttgcgctcg aaaaaaatat tgcaattgcc
     4561 attcattcca gagatgctac acatatttta attgatattt taaaaaaacg aaaacgaaat
     4621 ccaaaaggtg tttttcattg ttttacaggc agtgtggaag aagcgaaaga gattttgaag
     4681 ctgggttttt ttcttggaat tggcggtgtg gtgacgtata aaaacacaca tcttcgcgat
     4741 acattgctac aattgccact tgatagaatt gtattagaaa cagatgcgcc ttatttgcca
     4801 cctgtaccat atcgtggtaa gcggaatgaa agtgcttata ctaaaattgt cgcagaaacg
     4861 ttatgctcag tatatcaaaa agaatttgaa gaaattgcaa gtgtgacaac acaaaatgca
     4921 actgcattat ttggaattta gttgtaaatt taattaagct acaattatga aaaaagtatt
     4981 atttctgttt agtaccattt caattttttt gttttcgtgc acaaaaccac aagatgtgac
     5041 aggaccgaaa ttgattttta aattcaagtt tgattccacg caagcacgct taaataattt
     5101 tggacaaacc gtaactgttt tgccagtagg acatagtgct atgtcgccaa aatttcattt
     5161 aatgtcatct cattatattg agttagcacc tgattcatta tcgcctatcg gcactggtga
     5221 tatattgtat cgtgctgcag aagttggttc aggcgacgat ttagcaatcg atttttctaa
     5281 gtcagtaaaa gcaggcaata atgaagtgtt tttttctgtt ccattaaaaa atattacacc
     5341 aggtgcattt aagtggctgc gtgtttcctt agtttatcaa aattatgaca tcaaaataaa
     5401 atatgtaaat gatgccttgc ccattgcaaa tggttattac gatggcaatg ttgcgtcatt
     5461 tgttggtttt aatacctact tgaccaagta tacggtaaaa acacaacaag taacggtgaa
     5521 tgcggccaaa aaacaaggtt atggcgctgt tgaattattt aatttgcctt ctggatttcc
     5581 accgcaagca ccaatcgaat ttcaagcacc acccaatgct acaacggtgc ccaatccgat
     5641 tagtaatacc tcaccaatac cagcaggttc gtgtgtcgta acgggaagat ttgcacctat
     5701 aagtggattt gtaattactg gaaatgaaac caatgatatc gtgattactg tttctttgtc
     5761 aacaaataaa agttttgaat ggaaagataa aaatggcgat ggcttttatc agccattaaa
     5821 tggtgctaat aacactattg ttgaagatag tgtagtagat atgggcattc gcggattaat
     5881 tccaatta
//
LOCUS       DAOQQV010000144         5886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5882_length_5886_cov_5.394272, whole genome shotgun sequence.
ACCESSION   DAOQQV010000144 DAOQQV010000000
VERSION     DAOQQV010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5882_length_5886_cov_5.394272"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLL59_11360"
     CDS             complement(<1..1120)
                     /locus_tag="PLL59_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24450.1"
                     /translation="MRNKLLLLLLLFLSKSIFSQTIQLNSLSPTTFCATGKDTFKFAV
                     NYSNIPANSNIVFYQSTNPSFNPYAGQGDSIGFINVGSNTSNGGPQVITTCPEILGIF
                     LDACDPDPLEEQDNEYIVITSGQGFLVNNLQVDVPATSNRDINIPLGSNLFGTPSAAL
                     MAQLRIGSCNASNLIAAKPGDSIPPNAIVIIFPSVPLAGGNVYAYNFSSYCSSGQPIY
                     ILQNSYHQPNGSFANNPPGSCPGATRSTTIRNRSCVSNLVYNPCSLPAYDAGNPNAND
                     GNYAIKLPANAVSSSTNGGIRNNAADICNGLRFDSIVGNSTIKYPIPNDGSANVSTNF
                     CNTGTHYIKAITHPNASQPISNTLQFELICLDISTNVTS"
     gene            complement(1182..3878)
                     /locus_tag="PLL59_11365"
     CDS             complement(1182..3878)
                     /locus_tag="PLL59_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY24451.1"
                     /translation="MNKISSLYCTCFIVLLLFTFSTFAEPIINLGLSSPTVVCATGND
                     TTSYTVSASGIPANTNIVIYQSTDSTFNPYNNEGDSIGFIPGNAIPKDTINFGNCVKT
                     LGIFLDACGATGLEGRNEYIMLTSGNGIKVSNLAIDFSTQNNGSGPQNADINTNTNAC
                     GFKTPTPSLIAGLQVGSCNASNIIPASPSDSIPPNAIILCFTSDSVTASYSVNGLCNL
                     GYPIYVVQNACVRTIGAFTNASRCDAPSRYRTTKATDKRLNCTDNFTYDLCNIFDKDG
                     TYAIRQVGTDTASITNNGIRRNVIDSCGGIDYTQINFTADTTLKFRISPNFCNDGFHY
                     IKAITHPNGGQPISNTIKYKLVCNDISATTRNAIICSGESAIINSSSTNQQAQISWTV
                     SAGQNISGAVAGTGNSIQQILVNQSTIIDSVSYNISSNDAGCIKTTQVRIFVKGKVAL
                     SIDGDSTLCNNVGNTPLNAIGQFDSIRWNTGATSTSININQAGTYSVVAYANACSNNA
                     SVVVSNTTIETPIISGALEICDNEPTVLMVDNANNFDSIRWSNGAVGNQTSIQNVGAH
                     SLSVYKNTCSSVTRFVIEQCQAQDCNPTITGNQAFCQGDSIVLDAGAGFLSYAWNTGA
                     SSQTIKVKTPGKYLVNVTGQNCTGVDSVFVVVNELPQVTISGNTSICENGETVLTALA
                     DADSLRWNNGDTKPSLNVTQAQTYSVTVYKNGCSSQASVQVSVQATPSPFNLGNDTTF
                     CREFEKVLTTGNQSTTWSTGAQGAQITVTQAGTYIATVSNSCATVSDTIVITEEADCE
                     EGEIWLPNAFTPNGDGINDIFYVRGSGDMRSTLIETFRIYNRWGLKVFEVNNVHPEVK
                     SEGWDGKYKGEFAQDEVYGYFAIIKHSNGEKKTLKGNVTLLK"
     gene            complement(4039..5679)
                     /gene="groL"
                     /locus_tag="PLL59_11370"
     CDS             complement(4039..5679)
                     /gene="groL"
                     /locus_tag="PLL59_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HMY24452.1"
                     /translation="MSSKQISYSTESRDKLKAGVDALANAVKVTLGPKGRNVIIEKKF
                     GAPAITKDGVTVAKEIELEDPFENMGAQLVKDVASKTNDVAGDGTTTATVLAQAIIAP
                     GLKSITAGANPMDLKRGIDKAVTAVVKELKTISEKVGKDFSKIKQVGSISANNDEEIG
                     ALIAQAMEKVSTEGVITVEEAKGTETYVDVVEGMQFDRGYISPYFVTNADTMECEMEK
                     PFILIYDKKVSTMKDLLPVLEKVVQTGRGLLIIAEDVESEALATLVVNRLRGSLKIAA
                     VKAPGFGDRRKAMLQDIAILTAGEVISDELGLKLEDTQMNQLGVAEKVVIDKDNTTII
                     NGTGKKADIQARVNQIKAQIETTTSDYDKEKLQERLAKLSGGVAVLYVGAPSEVEMKE
                     KKDRVDDALHATRAAVEEGIVPGGGVALIRASIALDKLKGANEDEQTGIEIIKVALEA
                     PLRQISENAGVEGSIVVHNVKNGKKDYGYNAKTEVYENLFKAGVIDPAKVTRVALENA
                     ASIASMILTTECAIVEKPSKEAPAMPGGGMPGGMGGMM"
     gene            complement(5736..>5886)
                     /locus_tag="PLL59_11375"
     CDS             complement(5736..>5886)
                     /locus_tag="PLL59_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HMY24453.1"
                     /translation="VAVGPGKKDEPTTVKVGDTILYGKYSGTEINVDGNDYLIMRESD
                     ILAIV"
BASE COUNT         1715 a         1123 c         1033 g         2015 t
ORIGIN      
        1 agcttgttac atttgtagaa atatctaaac agatgagttc aaactgcaag gtattcgata
       61 ttggttggct agcatttgga tgtgtaatcg ctttgatata atgcgtacct gtattacaga
      121 aatttgttga cacattcgca ctgccatcat ttggtattgg atattttata gttgaattgc
      181 cgacgataga atcaaatcgt aagccattac aaatgtctgc ggcattattt cttattccac
      241 catttgttga agaagagaca gcatttgcag gtaatttaat ggcataattt ccatcattag
      301 catttggatt tccagcatca taagctggta atgaacatgg attatacact aaattgctta
      361 cacaagatcg attacggatg gtcgtagaac gcgtagcacc tgggcaagag ccaggaggat
      421 tgttagcaaa tgagccattg ggttgatggt aggaattttg caatatataa attggctgac
      481 cactactgca ataactacta aaattatacg catacacatt tccgccagct aaaggaacgc
      541 ttggaaaaat aataacaatt gcatttggcg gaatagaatc acctggtttg gcggcaatca
      601 aattactcgc attgcaggaa ccaattcgca gttgtgccat taatgctgca gaaggagtgc
      661 caaataaatt agagcctaaa ggaatattta tatctctatt cgatgttgca ggcacatcaa
      721 cttgcaagtt atttactaaa aatccttgtc ctgatgtgat aacgatgtat tcattatctt
      781 gttcttctaa tgggtctgga tcgcaagcat ccaaaaatat acctaatatt tctggacaag
      841 tagtaattac ttgcggtccg ccgttagaag tattactacc tacatttata aaaccaatgc
      901 tatcaccttg acctgcataa gggttgaaag atggattggt ggattgataa aacacgatgt
      961 tgctatttgc aggaatattg ctataattaa cagcaaactt gaaagtatct tttccagtag
     1021 cacaaaatgt agttggcgaa agactattta attgtattgt ttgtgaaaaa atactttttg
     1081 acaaaaacaa taataatagc aacagtagtt tgtttctcat tgtataaatt aggagtgcaa
     1141 aaataggcca attctacagc ttggccatta tctggaattt actattttaa taaagttaca
     1201 tttcctttaa gtgtcttttt ttctccatta gaatgtttaa taatggcaaa ataaccatac
     1261 acttcatctt gtgcaaattc acctttgtat ttgccgtccc aaccttctga ttttacttct
     1321 ggatgaacat tgttaacttc aaaaaccttt aaaccccagc gattatatat tctgaatgtt
     1381 tcaataagtg tactgcgcat atcgccgctc cctctaacat aaaagatgtc attaatgccg
     1441 tcaccgtttg gtgtgaatgc attgggtagc caaatttcac cttcctcaca atctgcttct
     1501 tctgtaatca caatcgtatc agaaacagta gcacaactat tggagacagt tgctatataa
     1561 gtaccagcct gcgtcactgt aatttgtgcg ccttgtgcgc ctgtgctcca agtagtggat
     1621 tgatttcctg ttgtcaatac tttttcaaat tctctgcaaa aagtagtatc gtttcctaaa
     1681 ttaaatggcg atggtgtagc ttgaacgctt acttgtacac ttgcttgtga agaacagcca
     1741 tttttatata ctgtgacgct atacgtttgt gcttgcgtta catttagcga tggttttgtg
     1801 tcaccgttat tccatctcaa tgaatccgca tcggcaagtg ctgtcaatac tgtttcacca
     1861 ttttcacaaa tgcttgtatt tccagatatg gtaacttgtg gcaattcatt aacaacaaca
     1921 aatacagaat ctactcctgt acaattttgt cctgtcacat taacaagata tttacctggc
     1981 gtttttactt taatagtttg tgaagatgcg ccagtattcc aagcatacga taaaaatcca
     2041 gctcctgcat ctaaaacaat agaatcacct tgacagaatg cttgattgcc cgtaatggta
     2101 ggattgcaat cttgtgcttg acattgttcg ataacaaacc gtgttacaga cgagcaagta
     2161 tttttgtaaa cgcttagcga atgtgcaccc acattttgaa ttgaagtttg atttcctaca
     2221 gcgccattgc tccatcttat ggaatcaaaa ttatttgcat tatctaccat taaaacagtt
     2281 ggttcattat cgcaaatctc gagtgcgcct gaaatgattg gcgtttcgat agttgtgtta
     2341 ctaaccacta cgcttgcatt gttagaacaa gcattagcat aggcaacaac actgtatgtg
     2401 ccagcttgat taatatttat ggacgtagat gttgctcctg tattccatct tatagaatca
     2461 aattgaccta ttgcgtttaa tggtgtgttg cctacattat tacaaagcgt tgaatcgcca
     2521 tcaatcgata aagctacttt tccttttaca aatattctga cttgtgttgt ttttatgcag
     2581 cctgcatcat ttgatgagat attataagaa acactatcaa taatagttga ttggttgact
     2641 aagatttgtt gtatggaatt gcctgtacca gctacagcgc cgctgatgtt ttgaccagcg
     2701 ctgactgtcc aactgatttg tgcttgttga ttagttgagc tactattaat tattgctgat
     2761 tcgccactgc aaattattgc attgcgtgta gtagctgaaa tatcattaca cactaattta
     2821 tatttaatcg tattggaaat cggttggcct ccatttggat gtgtaatggc ttttatataa
     2881 tgaaaaccat cgttacaaaa gttgggtgag atacgaaatt tcaatgttgt atctgctgtg
     2941 aagttgattt gtgtataatc gattccacca caagagtcaa ttacatttct tctgatacca
     3001 ttgtttgtaa tactagccgt atctgtgccg acttggcgaa ttgcatacgt tccatcttta
     3061 tcaaaaatat tgcataaatc ataagtaaaa ttgtcggtgc aatttaatcg tttgtcggtc
     3121 gctttcgttg ttcgatatct tgaaggtgca tcgcatcttg aggcgtttgt aaatgcgcca
     3181 atcgttctta cgcaggcatt ctgcactaca taaattggat agcctaagtt acataatcca
     3241 ttaacgctat aacttgcagt cacactatcg ctagtaaaac aaagtatgat ggcatttggt
     3301 ggaatggaat ctgatggaga tgctggaatg atattacttg cattacaaga accaacttgc
     3361 aaacctgcaa taagagaagg tgttggagtt ttaaaaccac aagcatttgt attggtattg
     3421 atatctgcat tttgaggacc actaccatta ttttgcgttg aaaagtcaat agctaaattg
     3481 gaaactttaa ttccatttcc agaagtaagc atgatgtatt cgtttcttcc ttctagtcca
     3541 gtggcaccac aagcatcaag gaaaatgcct aatgttttta cacaattacc aaaattgatg
     3601 gtatcttttg gtattgcatt tccaggaatg aaaccaatgc tgtcaccttc attattataa
     3661 ggattaaaag tactgtctgt gctttgataa ataacgatgt ttgtatttgc aggaatgcca
     3721 gatgcagaca cagtatagct tgtagtatcg tttccggtag cacagactac tgtcggtgat
     3781 gacaagccaa ggttaatgat aggctctgca aaagtggaaa aagtaaacaa taagagtact
     3841 ataaaacaag tgcaatacaa tgaagaaatt ttattcataa atcaatactt taaagccttg
     3901 aaagatacga agaatttatg gcaaatttgg taatttgttg aaaaacaaaa aagcggtgaa
     3961 taaatcaccg cttttaattg aattgcttat ttatatgata gcgtaaagca ttgtattttc
     4021 aaagaaaata catacctact acatcatgcc acccatacca ccaggcattc caccaccagg
     4081 cattgctgga gcctctttgc ttggtttttc aacgatagcg cattctgttg ttaaaatcat
     4141 agatgcgata gatgcagcat tttctaaagc aacgcgcgtt actttagctg gatcgataac
     4201 gcctgcttta aacaagtttt catacacttc tgtttttgca ttgtagccat aatctttttt
     4261 gccatttttc acattatgca caacgataga gccttcaaca ccagcatttt cagagatttg
     4321 acgcaatggc gcttctagag cgactttaat aatttcgata ccagtttgtt cgtcttcgtt
     4381 agcacctttt aatttatcta aagcaatgct agctctgatt aatgctacgc ctccacctgg
     4441 tacgatacct tcttcaacgg cagcacgagt agcatgtaat gcatcgtcaa cacgatcttt
     4501 tttctctttc atttccactt cagaaggcgc accaacataa agaacagcaa cgccaccaga
     4561 caattttgct aagcgctctt gtaatttttc tttatcgtaa tcagatgttg ttgtttcaat
     4621 ttgtgcttta atttggttga cacgtgcttg aatatctgct tttttacctg tgccgttgat
     4681 aattgtagta ttgtctttat cgataactac tttttcagca acgcctaatt ggttcatttg
     4741 tgtatcttct aatttaagac ctaattcatc agaaattact tcgccagcag ttaagatagc
     4801 gatgtcttgt aacatagctt ttcttctatc gccaaaacca ggtgctttta cggcagctat
     4861 ttttaaagag ccacgtaaac ggttaactac taaagttgct aaagcttcgc tttctacatc
     4921 ttcagcaatg attaataaac cacgaccagt ttgcactact ttttccaata ctggcaataa
     4981 gtctttcatc gtagaaactt ttttgtcgta gattaaaatg aaaggttttt ccatttcgca
     5041 ttccatagtg tctgcattcg ttacaaaata cggagagata taacctctgt cgaattgcat
     5101 accttctact acatctacat acgtttctgt accttttgct tcttctactg tgataacgcc
     5161 ttcagtactt actttttcca ttgcttgcgc tatcaatgca ccgatttctt cgtcgttatt
     5221 tgctgaaata gagccaactt gtttaatttt agagaaatct ttacctactt tttcagaaat
     5281 tgttttcaat tcttttacga cagcagtaac cgctttatcg ataccacgtt ttaaatccat
     5341 tggatttgcg ccagcagtaa ttgattttaa gccaggtgca ataatagctt gagctaaaac
     5401 agtagcagta gttgtaccat caccggcgac atcatttgtt tttgatgcta catcttttac
     5461 taattgagca cccatgtttt cgaatggatc ttctaattcg atttctttag ccactgttac
     5521 accatctttt gtgatggcag gtgcaccaaa ttttttctca atgattacat ttcttccttt
     5581 tggtcctaat gtaactttaa cggcatttgc taatgcatca acaccagctt ttaatttatc
     5641 tcttgattct gtgctataac taatttgttt cgagctcatt ttatttttat ttaagattgt
     5701 ttgaatttat aaattttctt gttgctgaca tttgattaaa caattgccaa aatgtcagat
     5761 tctctcatga ttaggtaatc atttccgtcc acattgattt ctgttccgct gtattttccg
     5821 tacaaaatag tatcgccaac tttaaccgtt gttggttcat cttttttacc aggacctact
     5881 gctact
//
LOCUS       DAOQQV010000145         5881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_5895_length_5881_cov_7.200137, whole genome shotgun sequence.
ACCESSION   DAOQQV010000145 DAOQQV010000000
VERSION     DAOQQV010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5895_length_5881_cov_7.200137"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLL59_11380"
     CDS             complement(<1..1003)
                     /locus_tag="PLL59_11380"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMY24454.1"
                     /translation="MQAIKTIVTKQCLRFCKLLFVCVSLIQTHHIFAQENSCANEIFQ
                     TIQLKDTAFIRQLTAISNNTSNNKPTASILEIPIVVHVLHLGEPIGTGSNIADQVIYD
                     AVRGANERWRKLNTTEGVDMEVQFCLAHTDPNGNATNGIVRINASSVPLYRDKGMGYS
                     AEILNGNFGADELQTKNLSNWRHNFVYNIWVVHKIVGDWGGYAYFPLPNGLDAIDGAV
                     LSANSMRYNSSTLAHELGHAMGLFHTFQGSENGCPANDLCFIQGDWICDTPPHKIADC
                     QNSVCNNSADSLFSFKNIMSYCGGRGMFTQGQKDRVRSTIFNSNRNQLLQSTACNGFS
                     "
     gene            complement(1037..2125)
                     /locus_tag="PLL59_11385"
     CDS             complement(1037..2125)
                     /locus_tag="PLL59_11385"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HMY24455.1"
                     /translation="MSAQVIEMETIPSIEIETTKESKLSKIDFDNMPFGKTFSDHMLI
                     CEYYDGKWQTAKIQPYQNISLSPACNVFHYGQAIFEGMKAYYKADESGIVLFRPKKNL
                     ERLNKSAHRMSMPEVPESLFMDGINKLIELDKDFIPKTAGSSLYIRPFLIGTEPFIGV
                     RTSDRYLFMVIIGPTGPYYGEAISVLVHDTYIRATKGGYGSAKAAGNYAGTLYPVELA
                     KNMGYKDVLWLDGEHKKYANEVGTMNIFFVIDGKLVTPNTDGSILEGITRDSIITIAR
                     DLGYTVEERPIDIDEITTAHEAGKLTEMFGTGTAAVVNPINRFGYKGKDYLLDDTAFE
                     IAPRVKTALVNIQKCIDQDKFGWITTVI"
     gene            2201..2620
                     /locus_tag="PLL59_11390"
     CDS             2201..2620
                     /locus_tag="PLL59_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24456.1"
                     /translation="MSTFFILNSSDETVLPDVKSDFVIFYKKSKEEAQANIQNELFSK
                     LIINALKLKLEDFLFVDISKPFMRFATIHKNITIKKCFLFGVSEKEVGINFDIPMYNL
                     LSINDIDFIKADTAEKLEADKQLKSQLWNQLVLTFKL"
     gene            2623..3198
                     /gene="rdgB"
                     /locus_tag="PLL59_11395"
     CDS             2623..3198
                     /gene="rdgB"
                     /locus_tag="PLL59_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HMY24457.1"
                     /translation="MRSLIFATNNPNKIKEISLQLSADFHIKNLIDIGCNEELPETHH
                     TIEENSAEKAIFVAKKFRVNCFSEDTGLEVEALNGEPGVDSAHYAGDRDANKNMDKLL
                     KKLETVGNRNAQFKTVITLIYKEVQYQFVGILKGKIGKEKKGNYGFGYDPIFVLADGR
                     TMAELMIDEKVQISHRAIATKKLIDFLSNLD"
     gene            3230..3808
                     /locus_tag="PLL59_11400"
     CDS             3230..3808
                     /locus_tag="PLL59_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMY24458.1"
                     /translation="MQPIEHKETLYNELIELCKRNNREAQYKLYSLLSSKMFAVCIRY
                     AKNKEAAEDLLQEGFVKVFTNIDKFRADGSFEGWVRRIMVNTAIEQYRKNTKMYPVVS
                     ADDIKIDIPETETSDNLEVEDLMKMINSLSHGYKTIFNLYVIEGFSHKEIADMLNISE
                     GTSKSQLARARYLLMEMVNKSAIVKRNATASE"
     gene            3789..5534
                     /locus_tag="PLL59_11405"
     CDS             3789..5534
                     /locus_tag="PLL59_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24459.1"
                     /translation="MQQRANNKFDKQVKKLLENAEVEAPAFLWNAIESKLPVQKPWYS
                     KYKYIILLLLLVSSSATSIFIYKNSQQHFGNNKNLATVNKQEQDDKNTSAAKKNGNTF
                     ENKSSNATSSNMLNTDALTSKPSVSNFYETSEGNKDNRLANIKQQQDKATAERTMRLK
                     RFEVLQANKIKDDKQNINTIQNTNVSNVSNSINQIQTPQNDIVIASATTLAKTTQTIS
                     DEQKILANTNQEMHDVNATNEKIQEANIASTHSNDNATEALIDDDSQNVFAVKNDSEK
                     NTNNLENSDYTATTEKENNNFSNFDATSKIVSPLNSSNAADLTASVIPVKINHTPIPS
                     RDVMRSLVAPEILEKFDFETQAAGINDLNPNREKILKNLKQFAGYNINRGFHIGAFIG
                     INSVWLNKKQFSTDENTTAIKPKFQLGKAYGINIGYDYSDRWGITMEWQIAEQGQKYS
                     MQQIGEDHRCVKEVNLLYTKFPILMKYKQMFINHYNSKPIALSFLFGPQVSLLLKKEI
                     LMDGNKIQSNAKYNKFEFGLIGGFDFDLFMTRNLSLTIGGRTGFGTSMQKGKPMSFQL
                     GVMTQLNFRLPKKIK"
     gene            complement(5622..>5881)
                     /locus_tag="PLL59_11410"
     CDS             complement(5622..>5881)
                     /locus_tag="PLL59_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24460.1"
                     /translation="ETLQNTNIVLQQIRKKYLIQCDYTITHAIFSLIQKVNATIEKQE
                     LEQQSTFVISIEQQYVLELEQQFEALKISLKHAMEIEKIEE"
BASE COUNT         2071 a          953 c         1013 g         1844 t
ORIGIN      
        1 aactaaaacc attacatgct gttgattgta ataattgatt tctatttgaa ttaaatattg
       61 tagagcgcac tctgtccttt tgtccttgcg taaacatgcc tctgccaccg caatagctca
      121 ttatgttttt aaatgaaaat aaactatcag cactattatt acaaacactg ttttggcagt
      181 cagcaatttt atgtggtggt gtatcgcaaa tccaatcacc ttgaataaag cataaatcat
      241 tggcagggca gccattttca cttccttgaa aggtatgaaa taagcccata gcatggccta
      301 attcatgcgc taaagttgag ctgttgtatc gcattgaatt tgcagataaa actgcgccat
      361 ctatggcatc taagccattt ggcaacggga aataagcata tccgccccaa tctccaacaa
      421 ttttatgcac gacccaaata ttataaacaa aattatgccg ccaattgctt aggttttttg
      481 tttgcaattc atcagcgccg aaattgccat ttaatatttc tgcagaatat cccatgcctt
      541 tgtctctgta aagcggcaca ctactcgcat tgatgcgaac aatgccgttt gttgcatttc
      601 catttgggtc tgtgtgcgca agacaaaatt gtacttccat gtcaacacct tccgttgtat
      661 tcagttttct ccagcgttcg tttgcacctc ttacagcatc ataaataact tgatctgcaa
      721 tattagaacc tgttcctatt ggttcaccta agtgtaaaac atgaacgaca attggaattt
      781 ctaagatact tgcggtaggc ttattattgc tagtattatt actaatagct gtcaattgtc
      841 tgatgaaagc ggtgtctttg agttgaattg tttgaaaaat ttcatttgca cagctatttt
      901 cttgtgcaaa aatgtgatgt gtttgtatta aagaaacgca tacaaataat agcttacaaa
      961 atcgtaagca ttgcttagtt acaatagttt taatggcttg catgataaat tatctattta
     1021 aagataatca tcatttttag ataacagtag ttatccagcc aaatttatct tggtcaatgc
     1081 atttttggat attcactaat gctgttttta cacgtggtgc tatctcaaaa gcagtgtcat
     1141 ctaataaata atcttttcct ttgtagccaa atcgattgat tggattgacc actgctgccg
     1201 taccagttcc gaacatttcc gttagtttgc cagcttcgtg tgctgttgta atttcatcaa
     1261 tgtcaattgg tctttcttcc accgtatatc ctaaatcgcg tgctatcgtg atgatgctat
     1321 cgcgtgtgat gccttctaat atgctgccat ctgtgtttgg tgttacgagc ttgccatcta
     1381 tcacaaaaaa tatattcatg gtgcccactt cattggcata ttttttatgc tcaccatcta
     1441 accataacac atctttatag cccatatttt tcgctaattc tacaggatat aatgttccag
     1501 catagtttcc tgctgctttt gctgagccat atccaccttt tgtagcacgt atatatgtat
     1561 cgtgcaccaa tacagatatt gcttcgccat aataaggtcc agttgggcca ataattacca
     1621 taaataaata acggtctgat gttcgaacgc caataaaagg ttccgtgccg attaaaaacg
     1681 gtcgaatata gagagaactt cctgctgttt taggaatgaa atctttatcg agttcaatta
     1741 atttattgat gccatccata aataaggatt ccggtacttc tggcatcgac atgcgatgtg
     1801 cggatttatt taatcgctct aaattcttct ttggcctaaa taacacaata ccgctttcat
     1861 cagctttata atatgctttc ataccttcaa atatggcttg accataatga aaaacattac
     1921 aagcaggact taaagaaata ttttgatatg gttgaatctt ggctgtttgc cattttccat
     1981 cataatactc acaaataagc atgtggtcag aaaaagtttt accaaatggc atattgtcaa
     2041 agtcaatttt agaaagtttg ctttcttttg tagtttcaat ctctatactt ggtatggttt
     2101 ccatttcaat aacttgtgca gacatgctaa aatattttat gcaaagttac aaaaaaaagg
     2161 tagttacttt gtggtttaca attaaaatta gtaaggtgtt atgagtactt tttttatttt
     2221 gaattcatca gatgaaacag tattgccaga tgtaaagtct gattttgtga ttttttacaa
     2281 aaaaagtaaa gaagaagcgc aggcaaacat tcaaaatgaa ttgtttagta agttaatcat
     2341 caatgcatta aaattaaaat tagaagattt tttattcgta gatatttcaa agcctttcat
     2401 gcgatttgct acaattcata aaaatattac aatcaaaaag tgctttttgt ttggtgtaag
     2461 cgaaaaagaa gttgggatta attttgatat tccaatgtac aatttattaa gcataaacga
     2521 tattgatttt ataaaagcgg acacagctga aaaactggaa gcagacaagc aattaaaaag
     2581 ccaactttgg aatcaattag tgcttacatt taaactttaa atatgcgatc cctaatattt
     2641 gctaccaata atcccaataa gataaaagaa atatctttac agctgtctgc cgactttcat
     2701 attaaaaatt taattgatat cggatgcaac gaagaactgc cagaaacaca tcatactata
     2761 gaagaaaatt ctgccgaaaa agcgattttt gtagcaaaaa aatttcgggt caattgtttt
     2821 tctgaagaca ctggcttgga agttgaagcg ctgaatggcg aaccaggcgt ggatagtgca
     2881 cattatgctg gagaccgaga tgcgaataag aatatggata aattactgaa aaaattggaa
     2941 acagtaggaa atcgtaatgc acaatttaaa acggtaatca ccttaatata caaggaagta
     3001 caatatcaat tcgtaggcat actcaaagga aaaataggaa aggagaagaa aggaaattat
     3061 gggtttggat atgatccgat ttttgtatta gcagatggca gaactatggc ggaattgatg
     3121 atagacgaaa aagtacaaat tagccacagg gcaattgcca ccaaaaaatt gatagatttt
     3181 ttgtcaaatt tagactaaat ttaaaataca cgcatttttt tttgctttta tgcaaccaat
     3241 cgaacataaa gagacactat ataatgagtt gatcgagctt tgcaaacgca ataatcgaga
     3301 agctcaatat aagctgtatt cacttttgtc gagtaaaatg tttgctgtat gcatacgata
     3361 tgccaaaaac aaagaagccg cagaagattt attgcaagaa ggctttgtca aggtttttac
     3421 aaatattgac aagtttagag cagatggctc ttttgaaggc tgggtcagaa gaataatggt
     3481 aaatacagcg attgaacagt ataggaaaaa tacaaaaatg tatccagtag ttagtgctga
     3541 tgacataaaa atagatattc cagaaacgga aacatcggac aatttagaag tagaagattt
     3601 gatgaagatg attaatagtt tgtcacatgg ctataaaacg atttttaatt tatatgtgat
     3661 agaaggtttt tcacataagg aaattgcaga catgttgaat ataagtgaag gtacatcaaa
     3721 atcacaattg gcacgagcta ggtatttatt gatggaaatg gtaaacaaat cagcaatagt
     3781 aaaacgaaat gcaacagcga gcgaataata aatttgataa gcaagtaaaa aagctgcttg
     3841 aaaatgcgga agtggaagcg cctgcgtttt tgtggaatgc tattgaaagc aagctgcctg
     3901 ttcaaaaacc ttggtattca aaatataaat acattatatt actactgctt ttagttagtt
     3961 cttctgcaac tagtattttc atttataaaa atagccaaca gcattttgga aataataaaa
     4021 atcttgcaac tgttaataag caagagcaag atgataaaaa cacatcagct gcgaaaaaaa
     4081 atggaaatac atttgaaaat aaatcttcaa atgctacaag ttcaaacatg cttaatactg
     4141 acgcgctaac atcaaaacca agcgtgagca atttctatga aacttcagaa ggcaataaag
     4201 acaatcgttt agccaatatt aaacaacaac aagataaggc tacagctgaa cgaacaatgc
     4261 gcttgaaacg ttttgaagtg ctgcaagcta ataaaattaa agacgacaaa cagaatatta
     4321 atactattca aaatacgaat gtttccaatg tttcaaattc gataaatcaa atacaaacgc
     4381 cacaaaacga cattgtaatt gcgtcagcaa ctacattagc taaaacgaca caaacaatat
     4441 ctgatgagca aaaaatatta gcaaatacaa atcaagaaat gcatgatgta aatgcaacaa
     4501 atgagaaaat tcaagaagct aatattgcgt caacacactc aaatgacaat gccacagaag
     4561 ctttaataga tgatgatagt cagaatgtat ttgcagtaaa aaatgattca gaaaaaaata
     4621 caaataactt agaaaattct gactatacag caacaacaga aaaagaaaat aacaattttt
     4681 caaatttcga tgcaacaagc aaaattgtga gtccactcaa cagtagcaat gcagctgatt
     4741 tgactgcatc tgtaattcct gtaaaaatta atcatacacc aattccatca agagacgtta
     4801 tgcgctcgct ggtagcacca gaaatattgg aaaaatttga ttttgaaaca caagctgctg
     4861 gcattaacga cttaaatcca aacagagaga aaatattaaa aaacctgaaa caatttgcag
     4921 gatacaatat caatcgaggt tttcatattg gcgcatttat tggtattaat agtgtttggt
     4981 taaataaaaa acaattcagt acagacgaaa atacgacagc tataaaacca aaattccaat
     5041 taggaaaagc gtacggcatc aatataggtt atgactattc agaccgttgg ggaattacga
     5101 tggaatggca gattgcagag caaggacaaa aatatagtat gcaacaaatc ggcgaagatc
     5161 atcgatgtgt aaaagaagtc aacttgcttt atacaaagtt tcccatttta atgaagtaca
     5221 aacaaatgtt tatcaatcat tacaattcaa aaccaattgc attgagtttc ttatttggtc
     5281 cacaagtgag tttgttgttg aaaaaagaaa tattaatgga cggcaataaa attcaatcaa
     5341 acgcaaaata taataagttt gaatttggtt taataggtgg ctttgatttt gatttgttta
     5401 tgacacgcaa tttatcattg acaataggcg gcagaacagg ctttggtact tctatgcaga
     5461 aaggaaaacc tatgagtttt cagttaggtg taatgacaca attaaacttc agattgccca
     5521 aaaaaataaa ataagttttt cacagttttt atagttttat ggtttgagcg ttcgacagaa
     5581 cgctcttttt atttttcatt ttactataaa taaatgcaag attattcttc tattttttct
     5641 atttccattg catgttttaa gctgattttt aaagcttcaa attgctgttc caattcaagc
     5701 acatattgct gttcgattga aatcacaaaa gtagattgct gttccaattc ttgtttttcg
     5761 atggtggcat ttactttttg gattaaagaa aagatggcat gtgttatggt ataatcacat
     5821 tgaattaaat atttttttct gatttgttga agtacgatat ttgtgttttg taatgtctct
     5881 t
//
LOCUS       DAOQQV010000146         5637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_6333_length_5637_cov_7.147080, whole genome shotgun sequence.
ACCESSION   DAOQQV010000146 DAOQQV010000000
VERSION     DAOQQV010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6333_length_5637_cov_7.147080"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PLL59_11415"
     CDS             complement(<1..1119)
                     /locus_tag="PLL59_11415"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMY24461.1"
                     /translation="MTQYLLKNISVLDTTSEFHGKKINVLIENNKAHFNVDASTATNA
                     TVVEFDNCFLSAGFCDIYTTTNDPGFESREDISSIANAAIAGGFTTICATANNHPITQ
                     TKAQVEYIINQSKQTPISILPIGAVTENFDGKTPTEMLDMHHAGAIAFADVPHSIKDS
                     GVLLRALQYAQAFDGLIIEMPFDKTLVGEGQVNESEISVRMGMKGITNLSEYAIVQRD
                     IELLKYANAKIHFTGISTKESVALIRKAKAENLNVTCSVFVHHLLADESAVKNFDANY
                     KVFPPLRSTTDQQALIDGIKDGTIDCISTQHTPLTVDEKNVEFEIADFGIIGLESAFG
                     LLNKRLENEIAKDKLVTLLSTNPRNIIQQNATSDFVILN"
     gene            complement(1116..3134)
                     /locus_tag="PLL59_11420"
     CDS             complement(1116..3134)
                     /locus_tag="PLL59_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HMY24462.1"
                     /translation="MSFIFPWFLLALLTLAIPVIIHLFYFRKYKKVYFSDIRFLKQVQ
                     EEKSTIDKIKKRWILAARLLALFFLVLAFVQPFIGKKNKQLSRSNSSIVVYVDNSYSM
                     GLKSGGEAALSIAKEKARELANAFSSNDKFALLTNELTGTHQRWMQKQNFLDAVEKIE
                     LSPTVKHINEIAQKQAALFQSVGDINKLGFFISDFQKNMLSKVDDTTFTQHFLQINGN
                     PEKNIFVDSCWFEQPVFTVNSTNKLMVRVKNAGNTTADKIRISVKINDAVKSIDDISI
                     PAENYIIDSFSFSITQAAWQRGEISFNDYPITFDDKFYFAFNVLSQEKVLSIEEKDTP
                     NNIASVFANDAHFIFDKTDAGQIDYSAFKNYALIILNQLHEISSGLAASLKEYLDNGG
                     NIYIIPNPIVSVQAYNTFLSTNNAGTYGELQTKSGEVTKINLQEDVFKNVFSILPKNV
                     ETPKINKYYPILSTTKSVARELLSTNLGSPLVAKFDAGNGFIYLQATPLNNTFSNLQS
                     MALFAPMVYNIAVFKKNKQALYYNIGKDNLIELKNENASNERVFTLSNGKYEFIPENR
                     TIGNTVLLRVNTNLPSDAHYSVNADGKTVAMAAFNFDRTESDIKFADKSELQALYKNK
                     NQIILDNTRTNLAAAVKHLKDGILLWKLCVILSLLFLLIEILLIRFWK"
     gene            complement(3276..4805)
                     /gene="lysS"
                     /locus_tag="PLL59_11425"
     CDS             complement(3276..4805)
                     /gene="lysS"
                     /locus_tag="PLL59_11425"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HMY24463.1"
                     /translation="MQLSEQEQIRREKLDKIKALGVDPYPAPLYPVNANAKEIKENYK
                     EEVLEDGTKNRLNFQEVCIAGRLMATRPAGKATFAELQDSTGRIQIYVRRDDICPTDD
                     KSNYDALFSKLLDLGDFIGIKGYVFVTKMGEISVHAKEFTLLAKSLRPLPVVKTDEEG
                     NVFDAFTDPEMRYRQRYVDLVVNANVRETFVKRTKMMNSIREFLNDKGALEVDTPVLQ
                     SIPGGAAARPFITHHNALDIPLYLRIANELYLKRLIVGGFDWVYEFSRNFRNEGMDRT
                     HNPEFTVLEFYVAYKDYFWMMETTEQLLEKAALDTNGNTYVQVGDNMINFKAPYKRIS
                     IYDAIQTYTGFDVSAMDEAGLRDVCKQLKIEVDNTMGKGKLIDEIFGARCEHNYIQPT
                     FITDYPVEMSPLTKKHRSKAGLVERFELMINGKEVANAYSELNDPIDQRERFEDQLKL
                     QERGDDEAMFIDQDFLRALEYGMPPTSGIGFGIDRLAMLLSNQASIQEVLFFPQMRPE
                     K"
     gene            complement(4911..5534)
                     /locus_tag="PLL59_11430"
     CDS             complement(4911..5534)
                     /locus_tag="PLL59_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HMY24464.1"
                     /translation="MQKITTLVFDLGAVILNLKSEQDWFLQDMFANFEQQQLLHLYQQ
                     HFFRDFEKGKITPADFIQQLSFIINDKSKSEQQIKSHWNSILLDIPMHRLTLLQQLKQ
                     KYRLLLLSNTNHIHVEHIRDYMQNDFGEDILHTTFDHCYFSQEIGFRKPDREIYEYVM
                     REQQLIPSEILFLDDKNENLQEPQKLGWHTFLVKYNNLTASDLHHLL"
BASE COUNT         1697 a         1008 c          958 g         1974 t
ORIGIN      
        1 attgagaata acaaaatctg acgttgcatt ttgttggata atatttcttg gatttgtgga
       61 aagcaaagtc actaatttat ctttagcaat ttcattttct aatcttttat tcaacagtcc
      121 aaatgcgctt tctaaaccaa taatgccgaa atctgctatt tcaaattcga catttttctc
      181 gtcaacagtc aatggtgtat gttgagttga aatacaatca atcgtgccat ctttaatgcc
      241 gtcaatcaat gcttgttggt ctgtagttga acgtaatggt ggaaatactt tatagttagc
      301 gtcaaagttt tttactgctg attcatcggc aagtaagtga tgcacgaaaa cagagcaagt
      361 aacattgagg ttttcggctt ttgctttgcg aatcaaggcc acactttctt tagtagaaat
      421 gcctgtgaag tgaattttag cattggcata tttcaacaat tctatatcgc gttgtacgat
      481 ggcatattcc gataaattcg tgataccttt catgcccata cgtacagaaa tttcactttc
      541 attcacttgt ccttcgccta ctaaagtttt atcaaatggc atttctataa tcaagccgtc
      601 gaaagcttgt gcatattgta atgcacgcaa tagcacaccg ctgtctttaa tactatgagg
      661 cacatcagca aaggcaatgg cgcctgcatg atgcatgtct aacatttctg tcggcgtttt
      721 tccatcaaaa ttttctgtta ctgcgccaat cggcaaaata ctgattggtg tttgtttaga
      781 ttggttgatg atgtattcta cttgtgcttt tgtttgtgta attggatgat tatttgcagt
      841 agcacagata gttgtaaaac cgccagcaat ggcagcattc gcaatagacg aaatatcttc
      901 acgactttca aatccaggat cgttggtcgt ggtataaata tcgcaaaatc ctgctgataa
      961 aaagcaattg tcaaattcta caactgtagc atttgttgcc gtcgatgcat ctacattaaa
     1021 atgagcttta ttgttttcta ttaaaacatt tatttttttt ccatgaaatt cggaagtagt
     1081 atctaagaca cttatatttt ttagtaggta ttgggtcatt tccaaaatct tatcagcaat
     1141 atttcaatta acaaaaataa cagcgacaaa attacgcaaa gtttccataa taatatgcca
     1201 tcttttagat gttttactgc tgccgctaaa ttagtgcgcg tattatctaa gattatttga
     1261 tttttgtttt tatacagcgc ttgcaattcc gatttatctg caaatttgat atcagattct
     1321 gttctgtcaa agttaaatgc agccatggca actgtttttc catcggcatt aacactataa
     1381 tgagcatcag atggcaagtt cgtatttaca cgcaacaaaa ccgtgttgcc aatcgtgcga
     1441 ttttctggaa taaactcata ttttccattg cttaacgtga aaactctttc attggaagca
     1501 ttttcatttt tcagttcaat tagattatct tttccaatgt tataatacaa tgcttgtttg
     1561 tttttcttaa atactgcaat attataaacc atcggtgcaa aaagtgccat cgattgcaag
     1621 ttggaaaatg tattgtttaa cggcgttgct tgtaaataga taaagccatt gccagcatcg
     1681 aattttgcta ctaatggtga acctaagttg gtagataaca actcgcgcgc aacagacttt
     1741 gtagtagaaa ggattggata atatttattg atttttggtg tttctacatt tttaggtaaa
     1801 atgctaaaga catttttaaa aacatcttct tgtaggttta tcttcgtaac ttctcctgat
     1861 tttgtttgca attcgccata agttcctgca ttattagtgc ttaaaaatgt gttataagct
     1921 tgtacagaaa ctattggatt tggtatgatg taaatattgc caccattgtc taaatattct
     1981 tttaaagatg ctgctaaacc agaacttatt tcatgtaatt gatttaaaat aattaaagca
     2041 taatttttaa aagcggaata atctatttga ccagcatctg ttttatcaaa aatgaaatgt
     2101 gcatcattag caaatactga tgcaatattg tttggtgtat ctttttcttc aatagacaat
     2161 actttttctt gtgacagcac attaaaagcg aagtaaaatt tatcgtcgaa tgtaattgga
     2221 taatcgttaa aggaaatttc gccgcgttgc caagctgctt gtgtaataga aaaactaaaa
     2281 gaatcaatga tataattttc ggcaggaata ctaatgtcat cgatagattt cacagcatcg
     2341 ttgattttta cagaaattcg aattttatct gctgttgtgt tgcctgcatt tttaacacgc
     2401 accatcaatt tattggtcga attcactgta aacacaggct gttcaaacca acacgaatcg
     2461 acaaatatat tcttttctgg attgccattt atttgtaaaa aatgctgtgt aaatgttgta
     2521 tcatcaactt tggaaagcat atttttttga aaatcggaaa tgaaaaaacc gagcttatta
     2581 atgtcgccta cgctctgaaa aagggctgct tgtttttgtg caatttcatt gatatgcttg
     2641 actgttggcg acagctctat cttttctacg gcatctaaaa aattttgttt ttgcatccaa
     2701 cgttgatgcg tgccagtaag ctcattcgtg agcaatgcaa atttatcatt agaagaaaat
     2761 gcgttggcta attctcttgc tttttcttta gctatagata atgcagcttc gcctccactt
     2821 ttcaagccca ttgaataaga attatcgaca tacaccacaa tagaggaatt gcttcttgaa
     2881 agttgcttat tttttttacc tataaatggt tgcacgaatg ctaagactaa aaaaaacaag
     2941 gccaataatc ttgctgcgag aatccagcgt tttttgattt tatcaatagt tgatttttct
     3001 tcttgtactt gctttagaaa acgaatatct gagaaataaa cctttttata ttttctaaaa
     3061 taaaaaagat gaataatgac aggaatcgcc agtgtaagta aagccaatag aaaccacgga
     3121 aaaataaaac tcatcttgtt gtaaagatag aaaatagttg atagtaaact gctcttgctt
     3181 aacagtattt aagactattc aaaagagatg aacttcgttg cacattgttc atttaatatc
     3241 ttctttttat tctttgtgca gcaagaaagt atgtcttact tctctggtcg catttgcgga
     3301 aagaatagta cttcttgaat agatgcttga ttgcttaata gcattgctaa tctatcaata
     3361 ccaaatccga tgcctgatgt tggtggcata ccatattcta atgcacgtaa gaaatcttga
     3421 tcgataaaca tggcttcgtc gtcgccacgc tcttgcagtt ttagctggtc ttcgaaacgt
     3481 tctctctggt caattggatc gtttaattcg gagtatgcat tggcaacttc tttaccattt
     3541 atcatcaatt caaaacgctc aaccaaacct gctttgcttc tgtgtttttt agttaatggc
     3601 gacatctcga caggatagtc tgtaataaat gttggttgaa tataattatg ttcgcatcga
     3661 gcaccaaata tttcatctat caactttcct ttccccatcg tgttgtccac ttctattttt
     3721 agttgcttac acacatcgcg caagcctgct tcatccattg ctgacacatc aaagccagta
     3781 taagtttgaa tagcatcgta aatagaaata cgtttatacg gcgctttaaa attgatcata
     3841 ttatcgccaa cctgcacata tgtattgcca ttagtatcta gcgctgcttt ttctaaaagt
     3901 tgctctgttg tttccatcat ccagaaataa tctttatatg caacgtaaaa ttctaatacc
     3961 gtaaattctg gattgtgtgt tctatccatg ccttcatttc taaagttacg cgagaactca
     4021 tatacccaat cgaaaccacc aacgataagt cttttcaaat ataattcgtt agcaatacgc
     4081 aagtacaatg gaatatctaa agcattgtga tgtgtaataa acggccttgc cgctgcgcct
     4141 cctggaatgc tttgcaatac tggcgtatct acctctaaag cacctttgtc gttcaaaaat
     4201 tcacggatgg aattcatcat cttagtgcgc ttcacaaagg tttcacgtac atttgcattt
     4261 accactaaat cgacataacg ttgacgatag cgcatttctg gatccgtgaa agcatcgaaa
     4321 acattgcctt cttcatctgt ttttacaact ggcaatggtc gcaatgattt tgctaataat
     4381 gtaaattctt tggcatgtac tgatatttcg cccattttgg ttacaaatac atagcctttt
     4441 atacctataa aatcacctaa atccaataat ttagaaaaca aggcatcgta attcgattta
     4501 tcatcagttg gacaaatatc atcacggcgc acataaatct gtattctgcc tgtactgtct
     4561 tgcagctctg caaatgtggc ttttccggca ggacgagtag ccatcaatct gccggcgatg
     4621 catacttctt gaaaattcaa tctgttttta gtgccatctt ctagcacttc ttctttataa
     4681 ttttccttta tttctttagc gtttgcattt actggataca gtggcgctgg atatggatca
     4741 acacctaaag cttttatttt atctaatttc tctcttcgaa tttgttcttg ttcagaaagt
     4801 tgcatatttt ttgaattgaa gtgcaaaaat aagattttta gacgatagtc cacaatctgc
     4861 gacgaataga ttatgacatc ttaacaatca gaaaaatatg tatcaaaata ttagagtaag
     4921 tggtgcaagt cacttgctgt taaattattg tatttaacaa gaaaagtatg ccagcctaat
     4981 ttttgtggct cttgcaaatt ctcatttttg tcatctaaaa ataaaatttc tgatggtata
     5041 agttgttgct ctcgcatcac atattcatat atttctctat ctggtttgcg gaagcctatt
     5101 tcttgagaaa agtagcaatg gtcgaatgtt gtatgcaata tatcttcacc aaaatcattc
     5161 tgcatatagt cgcgaatatg ctcgacatgg atatggttgg tattgctcaa taacagcaag
     5221 cgatatttct gttttaattg ttgtagcaaa gtaagtcgat gcataggaat atctaataat
     5281 atgctattcc aatgcgattt aatttgttgc tctgatttac ttttatcatt tatgataaat
     5341 gataattgct ggataaaatc tgctggcgta atttttcctt tttcaaaatc tctaaaaaag
     5401 tgttgctgat ataaatgtag tagttgctgc tgttcaaaat tggcgaacat atcctgtaaa
     5461 aaccaatctt gttctgattt tagattgaga ataacagcgc cgaggtcaaa tacaagtgtt
     5521 gtgatttttt gcatgagcta aaaataaaaa aaagctccga attggagctt tgaagtggtc
     5581 ccgcatgggc tcgaaccatg gaccccctga ttatgagtca ggtgctctaa ccagctg
//
LOCUS       DAOQQV010000147         5436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_6704_length_5436_cov_6.888497, whole genome shotgun sequence.
ACCESSION   DAOQQV010000147 DAOQQV010000000
VERSION     DAOQQV010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6704_length_5436_cov_6.888497"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /gene="rpsB"
                     /locus_tag="PLL59_11435"
     CDS             <1..422
                     /gene="rpsB"
                     /locus_tag="PLL59_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMY24465.1"
                     /translation="KKQNIEKMLGDGALNNITKKERLMLGRQQQKLDKVLGGIENLPR
                     IPAALFLVDITHEHIALAEAKNLGIKTIGIVDTNSDPTKVDFAIPANDDATKSIQLIT
                     NYIIEAIKEGLAERKKDKDEADSKTETDAKASVETAE"
     gene            516..1355
                     /gene="tsf"
                     /locus_tag="PLL59_11440"
     CDS             516..1355
                     /gene="tsf"
                     /locus_tag="PLL59_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HMY24466.1"
                     /translation="MSTATITASDVNKLRQETGAGIMDCKKALVETNGDFEAAIDYLR
                     KKGAKIAANRSDREANEGVVIALTSEDGKFGCAVNLSSETDFVAKNEDFINLAKSIAD
                     VALAGRIKSLEELQNAQLDGVSIKDKLLDTVAKIGEKIEVKKYEVLEGEGVVAYIHMG
                     YRMGVLVQLNETLNEAITLAGKDVAMQIAAMNPVAVDASGVSDELKERERAIAREKAI
                     AAGKPENILDKIADGAVQTMLKENTLLPQTFVKDGGKTVEQYLKSVDKDLTVVSFKRV
                     TLS"
     gene            1711..2421
                     /gene="pyrH"
                     /locus_tag="PLL59_11445"
     CDS             1711..2421
                     /gene="pyrH"
                     /locus_tag="PLL59_11445"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMY24467.1"
                     /translation="MNKPIKRVLLKLSGEALMGDQQFGIKAETLSIFADEIVEVIKAG
                     YQVGIVVGGGNIFRGLQAGNIGIERAQGDYMGMLATLINGMALQGAIESKGIYTRLLS
                     ALSIAQVCEPYIRRRAVRHLEKGRCIIMAAGTGNPYFTTDTAAALRANEIEADVILKG
                     TSVDGVYTADPKKDATASKYDTISYDEVMIKNLRIMDQTAFALCKENKMPIIVFNIKE
                     RGNILRILKGEKLGTLIS"
     gene            2562..3125
                     /gene="frr"
                     /locus_tag="PLL59_11450"
     CDS             2562..3125
                     /gene="frr"
                     /locus_tag="PLL59_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HMY24468.1"
                     /translation="MEEEVKMVVDETRDAMFKALKHLEEELRKVRAGKASADMLNGIT
                     VEYYGSPTPINQVASIKLLDARTLVIVPFEKKIINDIERSIFQSNIGITPQNDGENIR
                     LSVPPMTEERRRELVKQSKTHGEQTKIVIRNARKDGNEMVKDLVKEGLSEDMGKNVEE
                     TIQNLTNEYNTKVDKMLQVKEEEIMTI"
     gene            complement(3126..4490)
                     /locus_tag="PLL59_11455"
     CDS             complement(3126..4490)
                     /locus_tag="PLL59_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24469.1"
                     /translation="MKKSKFFELFTSFTKKQIQELSLMIEKNDDEQLITLCKHLNEFI
                     KGKMDDDAISRDYILKIVFKNKIDEKKLQKILNDGVKHCETLIIKYGVESDNHLQNLL
                     LANYYQKQQLDKYFNQQIQTAKETITTNKIDSNTFYYKYRFEHLSVNHELKSNLRKSN
                     YTDLSTYLHTYYETEQLKLKCVSYINLHDDLQHLPNSSVLFLQYQNCYTLLLSESEED
                     YIRFWNVLQAVVSEIDTEELKTIVNIFYNVCITQLNKNNSNYYEHLWNAYVFSLDNQI
                     ALEENGLLLPSHYKNIVTISLRLNKIDYALAFAEKYKSYLPEEHKNDVYNYNRAHIFF
                     YQKQYDDVLMLLVQSKFTDVFYKLSSRVLQIKTYVELSLLDHQYDDVLDSSMNALKKY
                     IYTNKEINELYASNYKNFYKALNKIISATPKTSENLIEEIKKIKPLPELEWLLMLLQK
                     SKKA"
     gene            4559..4927
                     /locus_tag="PLL59_11460"
     CDS             4559..4927
                     /locus_tag="PLL59_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24470.1"
                     /translation="MKIKSLLLISTFIFLHLSCSKDEALNKFKFTSIRGVKTGSTAVV
                     NFVWNDAQNTEWNVEVKNPDGTTRTVFTTAGTEGVINNLGLDTTYVFGVTGGTKTSQN
                     GSFNARIGTTGDVLIHDVKP"
BASE COUNT         1986 a          840 c          914 g         1696 t
ORIGIN      
        1 agaaaaaaca aaacatcgaa aaaatgttag gtgatggtgc tttaaacaac atcactaaaa
       61 aagaaagatt gatgttaggt cgtcaacaac aaaaattaga caaagtatta ggtggtattg
      121 aaaacttacc gcgtattcct gctgctttat ttttggtaga cattacacac gagcatattg
      181 cattagcaga agcaaaaaat ttaggtatta aaactattgg tatcgttgat acgaatagcg
      241 atcctacgaa agttgacttt gctattcctg caaatgacga tgctacaaaa tctatccaat
      301 taattacgaa ctatataatt gaagctatta aagaaggttt agcagagcgt aagaaagata
      361 aagatgaagc tgattctaag acagaaactg acgcaaaggc atctgtagaa acagcagaat
      421 aaaaagaaag taataaggaa aaagtagtag gtaattagaa aaatttattg ctttttcatt
      481 atcaaattaa ctcatcatca aattatcaaa ttatcatgtc aacagcaact ataacagcat
      541 ctgatgtaaa taaattaaga caagaaacag gtgcaggcat catggactgc aaaaaagctt
      601 tagtggaaac aaacggcgat tttgaagcag ccatcgatta tttaagaaaa aaaggcgcaa
      661 aaattgcagc aaatcgttca gatagagaag caaacgaagg tgtagtaatc gcattaacta
      721 gcgaagatgg caaatttggt tgcgctgtaa acttaagttc tgaaacagat ttcgtagcta
      781 aaaatgaaga cttcatcaac ttagcaaaat caattgcaga tgttgcttta gcaggtagaa
      841 ttaaatcatt agaagaatta caaaacgcac aattagatgg cgtatctatc aaagataaat
      901 tgttagacac agtagctaaa attggcgaaa aaatagaagt aaaaaaatac gaagtattag
      961 aaggagaagg tgtagtagct tatatacaca tgggttacag aatgggcgtt ttagttcagt
     1021 taaatgaaac attaaacgaa gcaattacct tagcaggaaa agatgttgct atgcaaatcg
     1081 cagcaatgaa tccagtagca gtagatgcat ctggtgtttc tgatgaatta aaagaaagag
     1141 aaagagcaat cgctcgtgaa aaagcaattg cagctggcaa accagaaaat atcttagata
     1201 aaattgcaga cggtgcagta caaacaatgt tgaaagaaaa cacgttgtta ccacaaactt
     1261 ttgtaaaaga tggaggaaaa acagtagagc aatacttaaa atcggtagat aaagatttga
     1321 cggtagtgtc gttcaagcga gttacattat cgtaatagtt acaagagaga ttttttaatc
     1381 tctctttttt tttgtttatt tttagaaaat atttatgaag caaaaattat tatttctatt
     1441 tcttattacc acatttactt tttcttgtaa aaatgcaaat gattgtgaaa agtgtatcat
     1501 taatattatt gataatagtg gtaatatagt ctcaacatat acaaaaacac aatttatttc
     1561 aaatgaccct aatggtaact ggtgtaattt tgttaataaa tcagaaacta cagagtttac
     1621 cgatgcttca ggtaattata ttggcaaagg tagaaaatct tgcaactaaa aaatctcatt
     1681 actcaatact aactactcaa tactaattca atgaacaaac caatcaaaag agtactttta
     1741 aaacttagcg gcgaagcctt aatgggcgac caacaatttg gaattaaagc agaaacactt
     1801 tctatttttg cagatgaaat agtagaagtc atcaaagcag gatatcaagt tggtattgta
     1861 gttggtggag gcaatatctt ccgtggtttg caagcaggaa atattggtat agaacgtgca
     1921 caaggcgatt atatgggcat gttagcaaca ctcataaatg gtatggcttt gcaaggtgct
     1981 atagaatcta aaggaattta cacacgcttg ctatctgctt taagcatagc gcaagtttgt
     2041 gagccataca ttcgccgtcg tgccgtgcgc cacttagaaa aaggacgttg catcatcatg
     2101 gctgctggta ccggaaatcc atattttaca acagatacag ccgctgcatt acgtgccaat
     2161 gaaatcgaag ctgatgtcat tttaaaaggc accagcgttg atggcgttta cacggcagac
     2221 ccaaaaaaag acgctactgc aagcaaatat gacaccattt cttatgacga agtgatgata
     2281 aaaaacttac gcatcatgga tcaaaccgca ttcgcattgt gcaaagaaaa caaaatgcct
     2341 atcatcgttt tcaatatcaa agaaagaggc aatatcttgc gcattttgaa aggtgaaaaa
     2401 ttaggcactt tgattagcta gtcaaagtat cttatttata aatgcgaatg ataagcgaat
     2461 ttttcattcg cattttttat ttgcataaat gtattttttt tgctaattgc attttgccta
     2521 ctatcaactt ttacgctatc tttgtggcat aatttttagc aatggaagaa gaagtaaaga
     2581 tggtcgtcga tgagacaaga gatgccatgt ttaaagcatt aaaacattta gaagaagagc
     2641 tgcgtaaagt acgcgctggt aaagcaagcg cagatatgct taatggcatt actgtagaat
     2701 actatggcag tccgacgcca atcaatcaag tggcaagcat caaattatta gatgcacgca
     2761 cattggtgat tgttcctttc gagaaaaaaa ttatcaacga tattgagcgc tctatctttc
     2821 aaagcaatat tggcatcaca ccacaaaacg atggcgaaaa cattagactt tctgtaccac
     2881 caatgacaga agagcgcaga cgtgaattag tgaagcaatc taaaacgcat ggcgagcaaa
     2941 ctaaaattgt tattcgcaat gcacgtaaag atggcaatga aatggtgaaa gatttagtaa
     3001 aagaaggctt gagtgaagat atgggaaaaa acgtggaaga aactatacaa aaccttacca
     3061 acgaatacaa cacaaaagta gataaaatgc tgcaagtgaa agaagaagaa attatgacaa
     3121 tataattacg cttttttact tttttgtaac aacattaaaa gccattccaa ctcaggtaat
     3181 ggctttattt ttttaatttc ttcaatcaaa ttttcagacg tctttggtgt agcacttatt
     3241 attttattta atgctttata aaaattttta tagttgctgg catataactc atttatttct
     3301 ttattggtgt agatgtattt ctttaatgca ttcatactgc tgtccaacac atcgtcatat
     3361 tgatgatcga gcaatgacaa ttcgacatac gtttttatct gcaacacacg actactcaac
     3421 ttatagaaaa catctgtaaa ttttgattgt actaacaaca tcaacacatc atcatattgt
     3481 ttttggtaga aaaaaatatg cgctctatta taattataaa catcgttttt atgttcttca
     3541 ggtaaatacg atttatattt ttctgcaaaa gctaatgcat aatcaatctt gtttaagcgc
     3601 aaacttatcg tgacaatatt cttataatgc gaaggcaaaa gtagtccatt ttcttctaat
     3661 gcaatttggt tgtcaaggct gaaaacatag gcattccaaa gatgttcgta atagtttgaa
     3721 ttatttttat tcaactgagt aatacataca ttgtaaaaaa tattcactat tgtttttaat
     3781 tcttcagtgt ctatttctga cacgacagct tgtagcacat tccaaaatcg aatataatcc
     3841 tcttcgcttt ctgacaaaag caatgtataa caattttgat attgcaaaaa caaaacacta
     3901 ctattgggca aatgctgtaa atcatcgtgt aaattgatat aagaaacaca ctttaatttc
     3961 aattgctctg tctcgtaata cgtatgcaaa tatgtactta agtctgtata gttgcttttt
     4021 cgcaaattgc tttttaattc atggttgaca gataaatgtt cgaaacgata cttgtaataa
     4081 aatgtattgc tatctatctt atttgttgtt atagtttctt ttgccgtttg aatttgttgg
     4141 ttgaaatatt tgtcgagttg ttgtttttga taatagtttg ccaataataa attttgtaaa
     4201 tgattatcac tttctacacc atatttgata attaaggttt cgcaatgttt aacgccgtcg
     4261 tttaaaattt tttgtagttt tttttcatca atcttatttt taaaaactat tttcagaata
     4321 taatctctac taatagcatc gtcatccatc ttccctttta taaactcatt cagatgttta
     4381 cataatgtaa taagttgttc atcatcattt ttttcaatca tcaaagagag ctcttgaatt
     4441 tgttttttag taaaggaagt aaacaattca aaaaacttag attttttcat aacaattgac
     4501 tttttgcaaa agaatacata atttcggaaa aattaaatta caatttaaat atgaaaatat
     4561 gaaaatcaaa tctctactac taattagcac atttattttt ttacacttga gttgtagcaa
     4621 agatgaggct ttaaacaagt tcaaattcac ttctattcga ggcgtaaaaa ctggctctac
     4681 tgctgttgta aattttgtat ggaatgatgc gcaaaacaca gaatggaatg ttgaagtaaa
     4741 aaatcctgat ggcaccacgc gaactgtatt tactacggct ggaacagaag gcgtaatcaa
     4801 taatctcggg ctcgatacaa cctatgtttt tggagttact ggcggaacaa aaacttctca
     4861 aaacggtagc ttcaatgcac gcataggcac cacaggtgat gtgcttattc atgatgtaaa
     4921 accataatta atggcaatat atttttcaaa aagcattcat ttttgaatgc ttttttttat
     4981 ttattttttt cagacattaa tgttaaagca aatttttggt aacacaatta actcattaaa
     5041 tatcaatatc ttacatcgtt tttatttaaa atgttatctt aaaaagtagc attattcttc
     5101 cgattagcgc agcatatttg taattttttt tagcgtattt ctatctcaat tttacatcat
     5161 caaacacaca acaaggaatg atgtaagtaa tccttttttt aaaaaaaact taccgccgaa
     5221 accgaaaagc gatattgagt aggaaataaa cttaaaaaat taaatacaat gaaaacatta
     5281 ttaaaaaaac aaatcggaaa aatcagtatc atggcattaa tcgtattaag ctgtatgagc
     5341 tgtaaaaaag aaaacattaa acatccaaca ccaaatgcag gcagctataa cataagcgaa
     5401 aacgccgcat ctggaattac agatcttatg tcacaa
//
LOCUS       DAOQQV010000148         5271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_7065_length_5271_cov_7.592600, whole genome shotgun sequence.
ACCESSION   DAOQQV010000148 DAOQQV010000000
VERSION     DAOQQV010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7065_length_5271_cov_7.592600"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PLL59_11465"
     CDS             <1..952
                     /locus_tag="PLL59_11465"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMY24471.1"
                     /translation="LEQPNTEYLRRLISTHKIVFIDEAQRIKNIGITAKIITDQFPEV
                     QLVLSGSSALDLNNELNEPLTGRKWELNLFTISMEELQQTIGFMQADQQLENRIIYGM
                     YPDVFNYPGNEKIVLNNLINSYLYKDVLALSGIKKPDMLQKLTQALALQIGNEVSYNE
                     LSQLLGINKETVSNYIELLEKTYVVFRVQPFSRNIRNEIKTSRKIYFYDTGLRNALIA
                     NFNPLELRVDKGVLWENFLISERIKFLKYHNIYTNTYFWRTAQQQEIDWIEEKDGIIT
                     AYEFKWHAKPKIKIPSSFINTYNATSKVIDKNNYYEFVSK"
     gene            1079..1729
                     /locus_tag="PLL59_11470"
     CDS             1079..1729
                     /locus_tag="PLL59_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24472.1"
                     /translation="MTTSIIIISGILIFFFLKRNNRRTPKNVNITAYNNSEDNFNEPI
                     ISYSIYTVDAHLNLRYLNYTHKDFPNHVGILKGQYVEIDWNILNADLITISGVGLVKA
                     IGRKAFYPTGNTSYTITAKNKTFEITQTIYVRVFPQNVTDKLFTSQPNTHIHSQQITK
                     IPELTKFPDFILNKPVIDTQKMKVINLHEVLQNKIIPTPFSQKVKDYFNNKQNINK"
     gene            1734..2912
                     /locus_tag="PLL59_11475"
     CDS             1734..2912
                     /locus_tag="PLL59_11475"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HMY24473.1"
                     /translation="MEEFKNNTSFPIHNVSGFTTFFWWCSGANQDVLKDCPQSEHNKY
                     VGIGATVFFTGVLATLSGGYALFSVFKSVYPAIAFGIVWGLVIFNLDRFIVSTLRKDE
                     LFWNEFKLVLPRLILAVILAIVISKPLELKIFEKEIAQKIEEQQREFAIQAQRKTEQQ
                     FNPVMDSLRSEIDLYKNEIAKMSTARDSLYTAFIGEAEGTRGTNMLGKGPVYKEKKQQ
                     YDKIELEMKELQAKYQPLIDEREQQILQAKAQRDTALAKAQPTIEGYDGLMARLDGLS
                     KLPQTPSWFIMLLFICIETAPVLSKLFSAKGPYDEKLKNIEHEIELNTIENINYRNHE
                     LNKKLTLTTNIDKAQIEQEIVNNKDALKIIADAHLELTKEMVNDWLETEKEKIKKDKV
                     "
     gene            complement(2958..4691)
                     /locus_tag="PLL59_11480"
     CDS             complement(2958..4691)
                     /locus_tag="PLL59_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HMY24474.1"
                     /translation="MKRDNLHSIFDIKKISFSLFFVLVASATLAQRSFYFPPIQKDTA
                     QKKKIEIIKSDSLIFQTLELGRYRKLIGNVKLKHIDAYMFCGSAIIDIEANYMTAYGK
                     QVHIKKGDSIDVWGNFLEYFGDQKFGRLTGMCSMRDNTMILTTPELNYNTETDVGSYM
                     SGGRIVNKETTITSAIGYYYHQQSTAIFYGDVVLKDKERTIHADSLRYNTTKEIAYFI
                     TKTTIIDKDSNIIVTNRGYYDTKLEKAFFGNNTEIKKGDASVKAETIDYDNKTKIAIA
                     KNNVVFQDSSERTTMLSNYIYSDENKSLVLAYRDPLMITVSENQKDTMYLSADTLLTY
                     KIASTDSLQVNDSISGNDSIKILKAWHHMKLIQGKMSAIADSLFYSESDSIFKMYYAP
                     ALWMDSTQITGDSIYLYNRNNEIYKVDIYNNAFIINLQDVEIYNQTKGKFMQAFLKNK
                     EMEKVDVDGNAESIYFIKDKENAYNGANKSSSAFITVSFDKGEVNRIKLTGTPEAEFT
                     PMKKINASTYRLDGFTWYGNKQPLTKYDVIRDKAQYEKYINGLPIKKVEIIEEKKEEN
                     SMETEQDNKEE"
     gene            complement(4766..>5271)
                     /locus_tag="PLL59_11485"
     CDS             complement(4766..>5271)
                     /locus_tag="PLL59_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24475.1"
                     /translation="GDLSATLVTVNKFAANADKMVVTLNGTLADNKANIDAIMSNIKS
                     LTSESGKLDSILTNFQTISGKVAALDLASTVESAKGALEQLKKTLSLINDGDGSISKL
                     LKEDGAYNDITKTIQSLNAVLTDLKANPKKYISLSLIDRTKTFTFTDTSVQQFLERHP
                     KAAKKIK"
BASE COUNT         1822 a          809 c          848 g         1792 t
ORIGIN      
        1 attagaacag cctaatactg aatacttacg tagattaata agtacacata agattgtgtt
       61 tatagacgaa gcacaacgta taaaaaatat cggcatcaca gcaaaaatta ttactgatca
      121 atttccagaa gtgcaactag ttttaagtgg ttcatcagca ttggatttaa acaatgaatt
      181 aaacgaacct cttacaggaa gaaaatggga attgaattta tttactattt ctatggaaga
      241 attgcagcaa actattggct ttatgcaagc agatcagcaa ttggaaaaca gaattatcta
      301 tggaatgtat cctgatgtat ttaattatcc tggaaatgaa aaaatagtat tgaataattt
      361 aataaacagt tatctgtata aagatgtatt agcattaagc ggtattaaaa agcctgatat
      421 gttgcaaaaa ctaacacagg cattggcatt gcaaatcgga aacgaagttt cctacaatga
      481 attgtcacag ttgttaggca ttaataaaga aacggtaagc aattatattg aattgctcga
      541 aaaaacatac gtggttttta gagtgcagcc atttagcaga aatattcgca acgaaattaa
      601 gacaagtagg aaaatatatt tttatgatac aggattacga aatgctttga ttgctaattt
      661 taacccactt gagttacgag ttgataaggg tgtgttgtgg gaaaattttc tgataagtga
      721 acgaatcaaa tttttgaaat accataacat atatacaaat acttatttct ggcgcacagc
      781 acagcaacaa gagatagatt ggattgagga gaaagatgga ataattacag cttatgaatt
      841 taaatggcat gcaaaaccaa aaataaaaat accttcttct tttatcaata cttataatgc
      901 tacatcaaaa gttattgata agaataatta ttacgaattt gtaagtaaat aatatcaatt
      961 ttcccttaaa attccttaaa attttccttt tcggaactat ctgtaatact atttagttta
     1021 ttccgacgta taaatagggt tgtaggtcgt gagcaataag tagtaaataa aaaaaatatt
     1081 gacaacaagc attattataa tctcaggcat attaattttc ttttttttaa aaaggaacaa
     1141 tagaagaacg cccaaaaatg ttaacattac tgcttataat aatagcgaag ataatttcaa
     1201 tgaacctatt atttcgtact cgatttatac agtcgatgct catcttaatc ttcgctactt
     1261 aaattacaca cacaaagatt ttccaaacca tgtaggcatt ctgaaaggtc aatatgttga
     1321 aatagattgg aatattttaa atgctgattt aattacaata agtggtgtcg gtttggtaaa
     1381 agccattggt agaaaagcat tttatccaac aggtaataca tcttatacta ttacagcaaa
     1441 aaataaaaca tttgaaatta cacaaacaat ttatgtgcga gtttttcctc aaaatgtaac
     1501 agacaaatta tttacatctc aaccaaatac tcacattcat tcgcagcaaa tcactaaaat
     1561 acctgagttg acgaaattcc ctgattttat tttaaataaa cctgttatcg atacgcaaaa
     1621 aatgaaagta atcaatttgc atgaagtatt gcaaaacaaa ataatcccaa caccttttag
     1681 tcaaaaagta aaagactatt ttaacaacaa acaaaatata aacaaatagc ataatggaag
     1741 aatttaaaaa caatactagt tttccaattc ataatgtaag tggttttact acattttttt
     1801 ggtggtgttc aggtgctaat caagatgtgc tgaaggattg cccacaatca gaacacaata
     1861 aatatgttgg catcggcgct acagtttttt ttactggcgt acttgccaca ttatctggtg
     1921 gctatgcatt gtttagtgta tttaaaagcg tatatccagc cattgcattt ggcattgtat
     1981 ggggtttagt tatttttaat ttagatagat ttattgtttc aaccttgcgt aaagacgaat
     2041 tattttggaa cgaattcaag ctggttttac caagattaat attagcagta attttagcta
     2101 ttgttatttc aaaaccatta gaattaaaaa tctttgagaa agaaattgca caaaaaatag
     2161 aagaacaaca aagagaattt gctatacaag ctcaacgcaa aacagaacaa caatttaatc
     2221 ctgtaatgga tagcttgcgc agtgaaattg atttgtataa aaatgaaata gcaaaaatgt
     2281 caactgcacg agattcttta tatacagcat ttataggcga agcagaaggc acgcgtggca
     2341 cgaatatgct aggcaaaggt ccagtatata aagaaaagaa acaacagtat gacaagatag
     2401 aattggaaat gaaagaattg caggcaaaat atcaaccatt gatagatgag cgtgagcaac
     2461 aaattttgca agcaaaagca cagcgcgata ctgcacttgc taaagcgcaa ccaacaattg
     2521 aaggttatga tggcttgatg gcaagattag atggtttatc gaaattgcct caaacaccaa
     2581 gctggtttat aatgttgttg tttatatgta ttgaaacagc accagtcttg tctaaattgt
     2641 tttctgcgaa aggaccttat gatgagaaat taaaaaatat agaacatgaa attgagttga
     2701 atactatcga aaatattaac tatagaaatc atgaactcaa taaaaaacta acgcttacta
     2761 ctaatattga taaagcacaa attgagcaag aaatagtaaa caataaagat gctttgaaaa
     2821 taattgctga tgcacattta gagctaacga aagagatggt gaatgattgg ttggaaactg
     2881 aaaaagaaaa aattaagaag gataaagtgt gataaaattt tatagaatgc agtagatatc
     2941 gccaaagagg aaaagtatta ttcttcttta ttatcttgtt cagtttccat tgaattttcc
     3001 tctttctttt cttcaatgat ttctactttt ttaataggca aaccattaat atatttttcg
     3061 tattgtgcct tgtctcgaat tacatcatat tttgtgagcg gttgcttgtt gccataccaa
     3121 gtaaaaccat ctaatctata agtagatgca tttatttttt tcattggagt aaactcagct
     3181 tcaggagtgc ccgtcaattt tattctgttt acctcgcctt tatcaaagga aactgtaata
     3241 aatgcagatg atgatttatt ggcaccattg tatgcatttt ccttgtcttt tataaagtaa
     3301 atactttctg cattgccatc tacatctact ttttccattt ccttattttt cagaaatgct
     3361 tgcatgaatt ttcctttagt ttggttgtaa atttcaacat cttgtaagtt gatgataaaa
     3421 gcattattgt aaatatctac tttgtaaatt tcattatttc tattgtataa ataaatcgaa
     3481 tcaccagtga tttgtgtgga atccatccat aaagcaggag cgtagtacat tttaaaaatg
     3541 gaatctgatt ctgagtaaaa aagtgaatca gcaatggcac tcatcttacc ttgaatcagc
     3601 ttcatgtgat gccatgcttt caatattttt atcgaatcat tgccgcttat actgtcgttc
     3661 acttggagag aatctgttga cgcaatttta taggttaata atgtgtcggc acttaaatac
     3721 attgtatctt tctgattctc agaaactgta atcattaatg ggtctctata ggcaagtact
     3781 aaggatttat tttcatcaga ataaatataa ttactcaaca ttgttgttcg ctctgaacta
     3841 tcttgaaaaa caacattgtt ctttgcaatg gcaattttcg ttttattgtc ataatcaatt
     3901 gtttctgctt tcacagaagc atcacctttt tttatttcag tattatttcc gaaaaatgct
     3961 ttttccaatt tggtgtcata gtagcctcta ttggttacaa tgatgttact atctttatct
     4021 attatcgttg tttttgtaat aaaataggca atttcttttg tcgtattata tcgcaatgaa
     4081 tcggcatgaa tagtgcgttc tttatctttt aatacaacat caccatagaa tatggctgtg
     4141 ctttgttgat gataatagta gccaatcgca ctggtaattg ttgtttcttt atttactatt
     4201 cggccaccac tcatatacga gccgacatca gtttctgtgt tataatttaa ctctggtgtt
     4261 gtcaaaatca tcgtattgtc acgcatggaa cacataccag ttaatcgacc aaatttttga
     4321 tctccaaaat actctaagaa atttccccaa acatcaatgc tatcaccttt tttgatgtgt
     4381 acttgcttgc cataggctgt catgtaattg gcttcaatgt caatgatggc gctgccacaa
     4441 aacatgtaag catcgatatg tttcagcttt acattgccga ttaattttcg atatcttcct
     4501 aactccaaag tttggaaaat aagtgaatct gattttataa tttctatttt tttcttttgt
     4561 gctgtgtctt tttgtattgg cggaaaataa aaagatcttt gtgccagcgt agcagatgcc
     4621 actaacacaa aaaataagga gaaacttatt ttcttgatgt cgaaaatact atgtaaatta
     4681 tctcttttca aaacaaataa aaaaagccat tgcaaataca atggctaaaa tacttttttt
     4741 ttggcattac ataaatgtat gcaatctact ttattttttt cgcagccttt ggatgtcttt
     4801 ctaaaaactg ttgtacagaa gtatctgtaa atgtaaatgt tttagttctg tctattaatg
     4861 acaagctgat gtattttttt ggatttgctt tcaaatcagt tagtacagca tttaaagact
     4921 gaatggtttt agtgatgtcg ttataagcgc catcttcttt taatagtttt gaaatcgaac
     4981 catcgccatc attaattaaa gaaagtgttt ttttcaattg ctctaatgcg cctttcgcac
     5041 tttctactgt cgatgctaaa tctaatgcgg ccactttgcc agatattgtt tggaagttag
     5101 tgagtataga atctaatttt ccggactcag aagttaagct tttaatatta gacatgatag
     5161 cgtcaatatt tgctttgtta tcagctaaag tgccatttaa agtgaccacc attttatctg
     5221 cattcgcggc aaatttattt acagtcacta aggttgcact taaatcgcct a
//
LOCUS       DAOQQV010000149         5167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_7306_length_5167_cov_8.394953, whole genome shotgun sequence.
ACCESSION   DAOQQV010000149 DAOQQV010000000
VERSION     DAOQQV010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7306_length_5167_cov_8.394953"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /gene="miaE"
                     /locus_tag="PLL59_11490"
     CDS             <1..320
                     /gene="miaE"
                     /locus_tag="PLL59_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HMY24476.1"
                     /translation="VVALQNFIKKGGSPAERFLDRLLLAALIEARSCERFRLLSLDIS
                     DDSLKHFYHKLMISEATHYRLFIDIAEQYFEKEVVKSRWTEWLDEETRILSTLELRGD
                     RIH"
     gene            330..1244
                     /gene="hemF"
                     /locus_tag="PLL59_11495"
     CDS             330..1244
                     /gene="hemF"
                     /locus_tag="PLL59_11495"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HMY24477.1"
                     /translation="MENVALPEIIAYLQTLQQSICEELENLDGHAKFIDDVWSRAEGG
                     GGLSKVIQQGNVFEKGGVNFSHVHGKLPDVLRTETRNAQYFHATGVSIVIHTQNPFVP
                     IIHMNVRYFSMSETENGDIVDEWFGGGIDLSPAYPNETDTKYLHTVLKDVCDTFDASY
                     YTTFKQWCDEYFTIKHRNEMRGVGGIFYDHLRPQSAAEKRTLFEFMQAVGNSFIPIYS
                     KIVKSNKDKVFTTQHRNWQYIRRGRYAEFNLVYDRGTHFGLKTNGRIESILMSLPPQA
                     AWTYNYVPEVDSEEEKTLPFFQPINWFL"
     gene            1358..2065
                     /locus_tag="PLL59_11500"
     CDS             1358..2065
                     /locus_tag="PLL59_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY24478.1"
                     /translation="MKQSFTKSECKHCSNRYNSVFCKTEHDSMEEIEAYKICSVFKKG
                     ETIFREGSYASGIYCINGGKIKLSMTGDEGKEQIIRMAKPGDIIGYKALLSSERYSAT
                     ATALEDCNVCFIPKEIFLAILQKDATLSFEMMKILSDELKRAEERITHLAQKPVRERV
                     AETILFLKETYGIDAENNINVALSREEIANLVGTATETVIRLLSEFNKDHIIELAGKR
                     IKILDKNKLVKTANLYD"
     gene            2181..2669
                     /locus_tag="PLL59_11505"
     CDS             2181..2669
                     /locus_tag="PLL59_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24479.1"
                     /translation="MLNLYHTDYNNFTSEELCVYLEERYYTQVLSDLEVIHKYLIDYS
                     YEDETDTAEIIIAIFRKLQIELKQLFTKDSILIFPHFKNKSDVTVNTDPFYQIHQRIN
                     DLLQQIRRKLNNYVQQPNWPTTLKICALELYSLEQLVQHVLYLKQNFLWPKINTVATS
                     EC"
     gene            2659..5088
                     /locus_tag="PLL59_11510"
     CDS             2659..5088
                     /locus_tag="PLL59_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HMY24480.1"
                     /translation="MSVDSNLKLSCYHCGEDCKDTSIQLQDKTFCCDGCKLVFEILNE
                     NELCAFYDINKNPGISQKIKVRPDKFAFLDDTSIQQKLIQFSDETQTHVTFYLPQMHC
                     SSCLWLLENIYKINKGVVSSKVNFENKEVFIIFNNNTTSLRKVVETLTQIGYEPHISL
                     HALDTSAVKKSDNKRIYKVGIAGFCFANVMMMSFPEYFVADGVVEANIRATLSYIIVA
                     FSIPVLLYCADEFFILAWKGIKHKFLNIDAPIALAITITFGRSIYEIFSHTGPGYLDS
                     MCGIVFFMLLGRILQDRTYQSISFDRDYKSFFPIAVNVIKEDKIIPTALYQLKKGDLI
                     QIYNNELVPADGILSKGKAQIDYSFVSGESIPVSKDIGEIIYAGGKQMAGLIELLVVK
                     DVSQSYLTNLWNKDAFQQKEDSNASFIHVIGKYFTVIVLGIATIAAAYWLYKGQYTLM
                     WNTITTVLIVACPCALLLSATFTNGNILRILSKNKFYLRHPDVIENITKINQLVFDKT
                     GTLTQQNKTKVSYNGQALSDIEQKNIASLLSQSTHPLSKAILDDLQINKISEVQNFKV
                     HVGKGIEGWIDDKHYKIGAYDFVYASSELSNTGSAVHIMIDNIWIGQYKIIHNYRFGF
                     NRLMEVLKAQYSIAVVSGDNDAEKNKLKEILGNDADLLFYQKPDDKLNYIQHLQNIQH
                     KNVMMIGDGLNDAGALKTSNVGVAVSENSNNFSPACDAILDASQFSNLDKFIAYAKSG
                     KKIILISFVLSIFYNVVGLFFAVQGTLSPLIAAILMPCSSITIILTTYGMSEWAAWKI
                     GLETKYDKSHV"
BASE COUNT         1807 a          772 c          934 g         1654 t
ORIGIN      
        1 atgtggtggc attgcagaat tttataaaaa aaggaggcag tccagcagaa cgctttttag
       61 atagattgct tttggcagct ttgatagaag cacggagctg cgagcgattt cgacttcttt
      121 ctttagacat tagcgatgat agcctaaaac atttctatca taaactcatg atatcagaag
      181 ccacacatta tcgtttattt attgacatag cagaacaata cttcgaaaaa gaagtagtga
      241 aatcgagatg gacggaatgg ctcgatgaag aaactagaat tttaagcaca ttagaattaa
      301 gaggtgacag aattcattaa atatagatta tggaaaatgt agcattgcca gaaattattg
      361 catatttaca aacattacaa cagtcgattt gtgaggaatt agaaaattta gatggtcatg
      421 caaaatttat cgatgatgtg tggagtaggg cagaaggtgg cggtggctta agtaaagtaa
      481 tacagcaggg aaatgttttt gaaaaaggag gtgtaaattt ttcgcatgtt catggtaaac
      541 ttcctgatgt actaagaaca gaaacaagaa atgcacaata ttttcatgca acaggcgtgt
      601 ccattgttat acatactcaa aatccttttg tgccaattat acacatgaac gtacgctatt
      661 tttctatgtc tgaaacagaa aatggtgata tcgtagatga gtggtttggt ggaggcatcg
      721 atttgtcgcc tgcttatccg aatgaaacag atacaaaata tctgcatacg gttttaaaag
      781 atgtttgcga tactttcgat gcttcatatt atacaacatt taagcaatgg tgcgatgaat
      841 attttaccat taaacacaga aatgaaatgc gtggtgttgg tggtattttt tacgatcatc
      901 ttcgcccaca gtcggctgct gagaaaagaa cgctttttga atttatgcaa gctgtcggca
      961 atagctttat tcctatttat agtaaaatag taaaaagcaa taaagataaa gttttcacta
     1021 cacaacatag aaattggcaa tacatcagac gcggcagata tgctgagttt aatttagtgt
     1081 atgatagagg gactcacttt ggattaaaaa caaacggaag aattgagtct attttaatga
     1141 gcttgccacc acaagcagct tggacctata attatgttcc agaagttgac agtgaagagg
     1201 aaaaaacctt gccttttttc cagcctataa attggttttt gtaatttttt aattttattt
     1261 ccaacatctt aataaataat cagatattct tatttattaa aggattaatt tatgcgtata
     1321 atacggttat tatgaaattt accactaaat ttgcaacatg aagcaatcct ttacgaaatc
     1381 agaatgcaaa cattgctcga atcgttacaa ttctgtgttt tgcaaaacag aacacgatag
     1441 catggaagaa atcgaagcgt ataaaatttg ttctgttttt aaaaaaggag aaacaatatt
     1501 cagagaaggt tcttacgctt ctggcattta ctgtatcaac ggtggaaaaa ttaaattgtc
     1561 gatgactggc gacgaaggaa aagagcaaat catacgcatg gcaaaaccag gcgatatcat
     1621 tggttataaa gccttgttgt ccagcgaacg atatagcgct actgcaaccg ctttggaaga
     1681 ttgtaatgtt tgttttattc ctaaagaaat attcttagcc attcttcaaa aagatgctac
     1741 attatcattc gagatgatga aaattttatc agatgaatta aaacgagcgg aagagcgcat
     1801 tacacatctt gcgcaaaagc ctgtacgcga gcgcgttgca gaaaccattt tgtttttgaa
     1861 agaaacgtat ggcatcgatg ctgaaaacaa catcaacgtg gctttatcac gcgaggaaat
     1921 tgcaaattta gttggcactg caacagaaac ggttattcgc cttctatcag aatttaataa
     1981 agaccatatt atcgagttgg ctggtaagag aattaaaatt cttgacaaaa acaagctcgt
     2041 taaaacggct aatctttacg attaatattt taacaaaatt ttcattaatt tttatgtaaa
     2101 tatgataaat atcatatttt gagttgatac acatcagtag tcttttctac tagagtatgt
     2161 acttttgaat aaaaattaca atgctcaact tataccatac agattataat aattttacat
     2221 ctgaagaatt gtgtgtttat ttggaagaaa gatattacac acaagtgctt agcgatttag
     2281 aagtaattca taaatatctg attgattatt cttacgaaga tgaaacagat acagctgaga
     2341 tcattattgc catttttaga aaattgcaaa tagaattaaa acaacttttt acaaaagata
     2401 gtattttaat atttccacat ttcaaaaata aatcagacgt aacggttaac acagatccat
     2461 tttatcaaat acaccaaaga atcaacgacc ttttgcaaca aatcagaaga aaactgaaca
     2521 attatgtgca acaacctaat tggcctacga ctttgaaaat ttgtgcatta gagttatatt
     2581 cgttagagca attagtacaa catgtgttgt atctaaaaca aaattttttg tggccgaaaa
     2641 taaatactgt agcaacaagt gagtgttgat tcaaatctta aattatcttg ttatcattgt
     2701 ggtgaagatt gcaaagatac atctatccaa cttcaggata aaactttctg ttgtgatggc
     2761 tgcaaattgg tgtttgagat attgaatgaa aatgaattat gtgcgtttta tgacatcaat
     2821 aaaaatccag gaatatcaca gaaaataaaa gtacgtccag ataagtttgc ttttttagat
     2881 gatacttcaa tccaacaaaa attaattcaa ttttctgatg aaacgcaaac gcatgttaca
     2941 ttctatttgc cacaaatgca ttgtagttcc tgtttgtggt tgctcgaaaa tatttataaa
     3001 atcaataaag gcgtagtgtc ttctaaagtt aattttgaaa acaaagaagt ctttatcatt
     3061 tttaataata atactacaag tcttcgcaaa gtagtagaaa cattaacaca aataggctat
     3121 gaacctcata tttctctgca tgcattagac acttcagcag taaaaaaatc ggataataaa
     3181 cgtatatata aagtaggcat cgctggcttt tgttttgcca atgtgatgat gatgagcttt
     3241 ccagaatatt ttgtggcaga cggtgtcgtg gaggctaata taagagccac tttgtcttat
     3301 atcattgtag cattttcaat accagtattg ttatattgcg ctgatgagtt ttttatttta
     3361 gcatggaaag gcataaaaca taaattctta aatatagatg cacccattgc tttggcgata
     3421 actattactt ttggccgtag tatttatgaa atcttctcac atactggacc tggatattta
     3481 gatagtatgt gcggcattgt tttttttatg ttgcttggta gaattttgca agatagaact
     3541 tatcagtcta tttcgttcga tagagattat aagtcatttt ttcctattgc cgtgaatgtg
     3601 attaaagaag ataaaataat tccgacagca ttatatcaac taaaaaaagg cgatctgatt
     3661 caaatttata ataatgaact agtgccagca gatggcatat tatcgaaagg aaaagcacaa
     3721 attgattata gttttgtgag tggtgaaagc attcccgttt caaaagatat tggcgaaata
     3781 atctatgcag gcggcaaaca aatggctggt ttaattgaac ttttagtggt taaagatgtt
     3841 tcacaaagct atctaactaa cctttggaat aaagatgctt ttcagcaaaa agaagactcg
     3901 aatgcatcgt ttatccatgt gataggtaaa tactttaccg tcatagtgct aggaattgct
     3961 actattgcag ctgcttattg gttgtataaa ggtcaatata cattgatgtg gaatacaatc
     4021 acaaccgtac ttatcgttgc atgtccatgt gcattgctct tgtcggcaac ttttaccaat
     4081 ggcaatattt tacgcatact cagtaaaaac aaattttatt tgcgtcatcc agatgttatt
     4141 gaaaacatta ccaaaatcaa ccagttagta tttgataaaa cgggtacatt aacgcaacaa
     4201 aataaaacga aagtttctta taatgggcaa gctttatccg acatcgaaca aaaaaatatt
     4261 gcttctctat tgtcacaatc tactcatcct ttaagcaaag caattttaga cgatctgcaa
     4321 ataaataaaa tttctgaagt gcaaaatttt aaagttcacg taggcaaagg aatagaaggt
     4381 tggattgatg ataagcatta taaaattggc gcttatgatt ttgtatatgc gagttctgaa
     4441 ctttcaaaca caggcagtgc tgtgcatata atgattgata acatttggat tggacaatat
     4501 aaaattattc acaattatcg cttcggattt aatcgattaa tggaagtgtt aaaagctcaa
     4561 tattcgattg ccgttgtttc tggtgataat gatgccgaaa aaaataaact gaaagaaatc
     4621 ttaggaaatg atgcagattt attattttac caaaaaccag atgataaatt aaattatatt
     4681 cagcatttac aaaacataca acataaaaat gtaatgatga taggcgacgg ccttaacgat
     4741 gctggcgcac tcaaaaccag taatgtcggc gtagctgttt ccgaaaattc caataatttc
     4801 agcccagcat gcgatgctat tttagacgca tcacaatttt caaatttgga taaatttatt
     4861 gcttatgcaa agtcgggaaa gaaaataata ttaattagct ttgtattgtc cattttttat
     4921 aatgtggtcg gtttgttttt tgctgtgcaa ggaacattat cgccactaat tgctgcaatt
     4981 ctaatgccat gtagttcaat tacaattatt ttaactactt atggcatgag cgagtgggct
     5041 gcatggaaaa ttggtttaga aacaaaatat gacaaaagtc atgtttagta atgatagcag
     5101 tcattaaaat aggcattgca gttttatatt tttgggcgta aatttaaaag atgagtgtaa
     5161 tcataat
//
LOCUS       DAOQQV010000150         5126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_7397_length_5126_cov_7.955630, whole genome shotgun sequence.
ACCESSION   DAOQQV010000150 DAOQQV010000000
VERSION     DAOQQV010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7397_length_5126_cov_7.955630"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLL59_11515"
     CDS             <1..634
                     /locus_tag="PLL59_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY24481.1"
                     /translation="DANTHWSTGATGPQISVTQAGTYIAENRNQCGSASDTIVIKQFA
                     APQVNIGRDTTICDSIQLSVGNGNFVSILWSTNDTTPSIIAHAQGIYTVTVRNQNCAK
                     TDSINIIKKCMYEVYIPTAFSPNADGFNDVYVPLTNLNGVQVLRFIIFNRWSEKVYEA
                     DNFTPNDITKGWNGTFKGEPAQQDNYVYYFTVKLPDNSEKTYKGTFALLR"
     gene            complement(684..3362)
                     /locus_tag="PLL59_11520"
     CDS             complement(684..3362)
                     /locus_tag="PLL59_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY24482.1"
                     /translation="MKKVLLVTLFLLPIFLKAQFLENKTLVDSFHQNIGAENTSYIRS
                     MKLMDNSICVLMHFGNFIQYKNQFRYDSVALHFYTIDGSKITTKKYALENYGYIEDFI
                     QLPSGDILLAGEVDTSIAFILLNQDGNYISTHTYSNVDSTNEIDNLYITNDNHVIMSS
                     NRKIIESQELCGVFPYFSFPMDLFEMQTYLTKIDFSGNTIWHKQIATDKRNGDISNYS
                     INNALLDILNNADHSFNIIKTKKDDFNGDYKLELLHIDKDGNLLNNTLLAENFTENNC
                     MSIFDNVSYKATSDGYFVFRKNIENNQFTRYHFNKAGILQDSAFQLNNTIGILPINPV
                     ELLQFNKEVFKSTFIHDFQYLEIATIMTSNQIQLTKLDSKYNIHWSETIYDSSCTQIG
                     NIGITSINDSTLLLISIVKDTSDQQEDISRLIFYTISLNANVIYYSTYIDKNNNAQFD
                     ATDTIFNAARIEVSSDSQTDIYPLDAYGTLFKHFKTGTYISKLISYEQNLRNFYVSPT
                     ADTTIFNSSNEIDSVFFRLVPIPNIQDLQVYIIPTNIARPGFYSSYKVVAQNVGSKTI
                     ENIEVKFLKDTFQTVGEFNPTPTHISNDTLIWTIDSLAIYENKSFYINCNNAAPPALN
                     INDTLTLFASITPTNNDTFIDDNTFELKQIIVNAYDPNDKTESHGAGISTQQVNNRDY
                     LYYTIRFQNIGTSAATKIKIVDTLSQNLDWSTLELLSNSHDLQYSVKNKNIITWQNDN
                     VYLPDSASDEAHSHGYVCFRIKPIASLTTSDVIENRAHIYFDFNTAVITNTVTTRIIE
                     QRVSSIKQQTIKAIKLFPTPTSNIVQLEFDMLLQRNLQLKVIDVNGNELQSQLLQTNT
                     GLNNILIDVSQYANGIYFIQINDASENRYYAKIVKQ"
     gene            complement(3617..>5126)
                     /gene="dnaK"
                     /locus_tag="PLL59_11525"
     CDS             complement(3617..>5126)
                     /gene="dnaK"
                     /locus_tag="PLL59_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HMY24483.1"
                     /translation="GQTVTEAVITVPAYFNDSERQATKEAGLIAGLDVKRIINEPTAA
                     ALAYGLDKGGKDMKIVVYDLGGGTFDVSILELGDGVFEVKSTNGDVHLGGDDFDQTII
                     DWLANEFQNEERIDLRKDPMALQRLKEAAEKAKIELSSATETEINLPYITAADGVPKH
                     LVKKLSRAKFDQLTENLVERTLEPCRKALKDAGISAGQIDEVILVGGSTRIPKIQEVV
                     EKFFGKKPSKGVNPDEVVAIGAAIQGGVLTGDVKDVLLLDVTPLSLGIETYGGVFTKL
                     IESNTTIPSRKSETFSTASDNQPSVELNVLQGERPMAKDNKSLGRFHLDSIPPAPRGV
                     PQIEVTFDIDANGILSVTAKDKGTGKEQNIRITASTGLSKEEIEKMKAEAKANEAADK
                     EARAKVDKLNEADSLIFQSEKQLKEFGDKLPADKKATIETAVVKLKEAHKTQDIAAID
                     AALAELNNAWQSASQDMYNASQGGAQQDGADHNANNNATDDVQDAEVVEEVK"
BASE COUNT         1551 a          857 c          851 g         1867 t
ORIGIN      
        1 agatgccaat acacattgga gcacaggcgc aacaggacca caaatttctg taacacaagc
       61 aggtacttat attgcagaaa atagaaatca atgtggcagt gcaagtgata caattgttat
      121 taaacaattt gctgcaccac aagtaaatat tggtagagat acgacaattt gcgatagcat
      181 acagttaagt gttggcaatg gaaattttgt ttccattctt tggagtacga atgatactac
      241 gccttccatt attgcgcatg cacaaggcat ttacaccgtc actgtgcgca atcaaaattg
      301 tgcaaaaaca gacagcatta acattatcaa aaaatgcatg tacgaagtgt atattccaac
      361 agcattttcg ccaaatgctg atggatttaa tgatgtatat gtgccattga ctaatctaaa
      421 tggcgttcaa gttttgcgtt ttattatttt caatcgctgg agcgaaaagg tatatgaagc
      481 agataacttt acaccaaatg atatcaccaa aggttggaac ggcacgttta aaggcgagcc
      541 agcacaacaa gataattatg tgtattattt cacagtaaaa cttccagata atagcgagaa
      601 aacatataaa ggaacttttg ccttattgcg atagtttttt tgttgaagct atttttgttt
      661 ttatcaatct aaatagaact ttcttactgt tttacaattt tagcgtagta tctattttca
      721 gacgcatcat tgatttgtat aaaataaatt ccgtttgcat attggctcac gtcaatcaaa
      781 atgttgttaa gaccagtatt ggtttgcaat agttgacttt gtaattcatt gccattaaca
      841 tcaattactt ttagttgtaa gttgcgttgc aacaacatat caaattccaa ctgaacgata
      901 ttgctcgttg gcgttggaaa tagcttgatt gctttaattg tttgttgttt aatactagat
      961 acacgttgtt ctataatgcg tgtagtaaca gtattagtaa tgactgctgt attaaaatca
     1021 aaataaatat gagctctatt ttcaatgaca tcagaggtgg ttaaactggc tattggtttt
     1081 attctaaaac atacataacc atgactgtgc gcttcatcac tggcactgtc cggtaaatat
     1141 acattgtcat tttgccaagt gataatattt ttatttttta cactgtattg taaatcgtga
     1201 gaattgctta ataattctaa agtactccaa tctaaatttt gggaaagcgt atctacaatt
     1261 ttaattttgg ttgcagcgct agttcctata ttttgaaaac gaatagtata atatagataa
     1321 tctctattgt ttacttgttg cgtgcttata cctgcgccat gtgattctgt tttgtcatta
     1381 gggtcatacg cattaacaat aatttgtttt aattcaaaag tattatcatc aataaaagta
     1441 tcattattag taggcgtgat gctggcaaat aaagttaaag tatcatttat gtttaacgct
     1501 ggaggtgctg cattattaca gttaatgtag aatgatttat tttcataaat agcaagcgaa
     1561 tcaattgtcc aaatgagcgt gtcattactg atgtgtgtag gtgttggatt aaattctcca
     1621 acagtttgaa atgtgtcttt caaaaatttg acttcaatat tttcgatggt tttcgaacca
     1681 acattttgtg cgacaacttt atatgatgaa taaaatcctg gccttgctat atttgttgga
     1741 atgatataaa cttgcaagtc ttggatattt ggaataggca ctaatcgaaa aaaaacagaa
     1801 tcaatttcat tgctgctatt aaaaattgta gtatcagctg ttggcgaaac gtagaaattg
     1861 cgtaagtttt gttcgtatga aattagcttt gaaatatagg tgcctgtctt gaaatgttta
     1921 aatagcgtac catatgcgtc taatggataa atatccgttt gagaatcact acttacttct
     1981 attcgtgccg cattgaatat agtatcagtt gcatcaaatt gtgcattatt gtttttatcg
     2041 atgtaagtcg aataatatat aacattggca ttgagtgaaa tggtgtaaaa tattaatcgt
     2101 gatatatctt cttgctgatc gcttgtgtct ttcactatac tgatcagcaa caaagtactg
     2161 tcgttgatac ttgttatgcc aatatttcca atctgtgtgc aggaactatc atatatcgtt
     2221 tcagaccaat gtatgttgta cttactatct aatttagtga gttgtatttg gtttgatgtc
     2281 ataatggttg ctatttctaa gtattgaaag tcatgtatga atgtactttt aaatacttcc
     2341 ttgttgaatt gtaataattc cacaggatta ataggcagta ttccaatagt attgttcaat
     2401 tgaaaggcag aatcttgtaa tattcctgct ttgttaaaat gatatcttgt aaattgatta
     2461 ttttctatat tttttctaaa aacaaaatat ccgtcacttg tagctttata gctaacattg
     2521 tcaaaaattg acatacaatt attttcagta aaattttcag cgagtaaagt gttatttagt
     2581 aggttgccat ctttatcaat atgcaataac tctaatttat agtcaccatt aaaatcatct
     2641 ttttttgttt ttatgatatt aaacgaatgg tctgcattat ttaaaatatc aagaagtgca
     2701 ttgtttatgg aatagttaga tatatcacca tttcttttat ccgttgcaat ttgtttatgc
     2761 caaatagtat taccggaaaa gtctatttta gtaagatacg tttgcatttc aaacaaatcc
     2821 attggaaatg aaaaatatgg gaatacacca cacaactcct gactttctat aattttacga
     2881 ttactactca taattacatg attgtcattt gtgatataga gattgtctat ttcatttgtc
     2941 gaatctacat ttgaataagt atgcgtacta atataatttc catcttgatt tagtaatatg
     3001 aatgctatgc tagtgtccac ttcaccagca agcaatatat cgccacttgg caattggata
     3061 aaatcctcta tgtagccata gttttcaagt gcatattttt tagtagtaat tttcgatcca
     3121 tctattgtat agaagtgaag cgcaacactg tcatatcgaa attgattttt atattgaata
     3181 aaatttccaa aatgcataag cacacaaatg gaattatcca ttaatttcat tgatctgata
     3241 taactcgtat tttctgctcc gatattttga tgaaagctgt ccaccaaagt tttattttcc
     3301 aaaaattgtg cttttaaaaa tataggcaat aaaaacaaag tcactaataa tactttcttc
     3361 ataatatgta tttagatgag acaggataat ttcaaattta tacaatttat ttatcattat
     3421 gaggtacaaa tttttgcctt tgtctgtttt tttagaagat aaacttattt ctaattgttt
     3481 gattattaaa gttatgtgta taatttttgt tgatgttttg tcaataaaaa cggattgctt
     3541 gtaatattat aatataataa aaaagccatt ttacgtaagt aaaatggctt tagaattaat
     3601 aagtgtttaa aatgaattat ttcacttctt ctacaacttc tgcatcttga acatcgtcag
     3661 tagcattatt atttgcattg tggtcagcac catcttgttg tgcgccacct tgcgaagcat
     3721 tgtacatatc ttgcgaagca gattgccatg cattatttaa ttctgctaag gctgcatcta
     3781 ttgctgcaat atcttgtgtt ttatgtgctt cttttaattt gacaacagct gtttcgatag
     3841 ttgctttttt gtcagcaggt aatttgtcgc caaattcttt taattgtttt tcactttgga
     3901 aaatcaaact atctgcttcg ttaagcttat ctacttttgc acgcgcttct ttgtcagcag
     3961 cttcattagc ttttgcttcc gctttcattt tttcaatctc ttctttgctt aagccagttg
     4021 atgctgtgat gcggatattt tgctctttgc cggtaccttt atcttttgct gttacactca
     4081 aaataccatt cgcatcgatg tcaaaagtca cttcaatttg aggcacgcca cgtggcgctg
     4141 gtggaatgct gtctaagtgg aaacggccta agcttttgtt gtctttcgcc attggacgtt
     4201 cgccttgcaa tacattcaat tccacagaag gttggttgtc tgatgctgta gagaaagtct
     4261 ctgattttct tgaaggaata gtcgtattgg attcaatcaa tttggtgaat acaccaccat
     4321 acgtttcgat acctaatgat aatggcgtaa cgtctaacaa taaaacatct ttcacatcgc
     4381 cagttaaaac accaccttgg atggcagcgc caatagctac tacttcgtca ggatttacac
     4441 ctttacttgg ttttttgcca aagaattttt ctactacttc ttgaattttt ggaatacgtg
     4501 ttgaaccacc aactaagata acttcgtcaa tttgaccagc tgaaattcct gcatctttta
     4561 atgcttttct acaaggttct aatgtgcgtt caaccaagtt ttctgtcaat tgatcgaatt
     4621 ttgcacgcga taattttttc actaagtgtt tcggcacacc atcagcagca gtgatatatg
     4681 gcaagttgat ttctgtttct gttgcagagg acaattcaat ttttgctttt tctgcagctt
     4741 ctttcaagcg ttgcaatgcc attggatctt tgcgcaaatc gattctttct tcattttgga
     4801 attcattcgc caaccaatca atgatagttt ggtcaaaatc atcaccacct aaatgcacat
     4861 cgccattcgt agattttact tcaaatacgc catcacctaa ctctaagata gatacgtcaa
     4921 atgtaccacc acctaagtca tacaccacga ttttcatatc tttgccacct ttgtctaagc
     4981 catacgctaa agccgccgca gttggttcgt tgatgatacg tttcacatct aaaccagcaa
     5041 ttaaaccagc ttcttttgta gcttgacgtt cagagtcgtt gaagtaagca ggcactgtaa
     5101 tgacagcttc tgtaacagtt tgacct
//
LOCUS       DAOQQV010000151         4889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_7932_length_4889_cov_8.134464, whole genome shotgun sequence.
ACCESSION   DAOQQV010000151 DAOQQV010000000
VERSION     DAOQQV010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7932_length_4889_cov_8.134464"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLL59_11530"
     CDS             complement(<1..377)
                     /locus_tag="PLL59_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMY24484.1"
                     /translation="MNHLLDIQEQTILAVEKAATFIKSEIGKTSNSDIETKALNSLVT
                     YVDKEAEKILVRELQKIMQDATFLTEENTIATTHGEWQWIIDPLDGTTNFIHQVPVFG
                     ISVGLKHKDEIVVGVVQELNRNE"
     gene            complement(378..1094)
                     /gene="rsmI"
                     /locus_tag="PLL59_11535"
     CDS             complement(378..1094)
                     /gene="rsmI"
                     /locus_tag="PLL59_11535"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HMY24485.1"
                     /translation="MSKLYIVPTPIGNLQDITLRAIDTLKSVQLILAEDTRTSKFLCQ
                     HFLIDTPLQAYHKDNEHKAVDAIVQKIKSGVEMALVSDAGTPAISDPGFLLVRACLQN
                     DIAVECLPGATAFVPALVNAGFPTDRFVFEGFLPHQKGRQKRLQALQEETRTIVFYES
                     PYRIVKLLEELNAYFGGNRRIAISRELTKKFEENFRGSIAEAIIHFTQKEPKGEFVVV
                     LEGKTKEEHVAISNKEKYGK"
     gene            complement(1104..1676)
                     /locus_tag="PLL59_11540"
     CDS             complement(1104..1676)
                     /locus_tag="PLL59_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24486.1"
                     /translation="MHTIEPYWKWRDFYTAEEDEKSPFYGKEYSEFEYSDTIYNYYIH
                     PQWDSFGAETLYLKLLYANYKTGAAIIEFIGEWNDCTQNTIETLKRDFIDILLHQGIS
                     KYVLIGENVLEFFSSDSEYYEEWYDDIKDNAGWIIAINFREHVLAEMRQANIHHYINI
                     NERYATINWRSYKPFHLISFLDDLLIKVIQ"
     gene            complement(1741..2604)
                     /locus_tag="PLL59_11545"
     CDS             complement(1741..2604)
                     /locus_tag="PLL59_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24487.1"
                     /translation="MILFADSGSTKTSWLLYNEADNTKKHFETLGINPIVHKHEEIYQ
                     KIYSNTELTAYANDITEIKFFGAGCSSVERNQLAASALKSIFYNASIEIDHDMKAAAY
                     AVCGNEPGIACILGTGSNSIFFDGENLVDSYAGIGYILGDEAGGAYFGKIILRDFLYH
                     LLPQEIEEHILSAYKLDKNTIFQLVYKEASPNRYLASFAPILSKFRETDYAQVVLQQG
                     FTEFFDFHITCFENYQDYPIGFVGSIAKTFRPELERVANDFECTLGKFVSNPIEEVAD
                     YFIQKKLAANL"
     gene            2672..3322
                     /locus_tag="PLL59_11550"
     CDS             2672..3322
                     /locus_tag="PLL59_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HMY24488.1"
                     /translation="MKTALVIGASGLVGGHVVSQLLNEQEFSAISILTRKPLHLQHPK
                     LKEIIVDFDNLENYATQIKADCVFCCLGTTIKTAGTQAAFRKVDFEYPLKVAEIAKQN
                     GTTTFILISALGASKNSFIFYNRVKGEIEEAISQLQFDSFHILQPSLIIGERKDTRLG
                     EGIAQKLSPVYDVLMFGPLSKYRSIKAEQIAKAMVEYAKSDGKGVFRHDSGALQQV"
     gene            complement(3349..4287)
                     /locus_tag="PLL59_11555"
     CDS             complement(3349..4287)
                     /locus_tag="PLL59_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMY24489.1"
                     /translation="MIRILIAVFAISIIACEKNDDIQVPPALYDSMVYEKTDIDILEV
                     SFTARPNFHNLQYTSEKTKQAEINKPTLTAKMDIYLPPNASSTSKQPLLVMIHGGGYS
                     SGDKQAWQNEAYTYARAGYICASLNYRLTQNGGNQDSLLRLYAVQCAVEDIQNGIRFL
                     KKNANLYFIDTTRIVVFGGSAGGGLSLINAIEYDAPFGTNDHVGFSSKTNGSISTGAT
                     LINDDAASQQGVLHYDANDSPVLLFHAKENDGSTGATWTNSVLPTQKAINNSGNSCTI
                     VAQPNLSHTVELALGGDYWNDIKPFLETHLRIATIR"
     gene            complement(4382..>4889)
                     /locus_tag="PLL59_11560"
     CDS             complement(4382..>4889)
                     /locus_tag="PLL59_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMY24490.1"
                     /translation="SVKPDILVLGKALSGGTMPVSAVLARDEIMLCIKPGEHGSTFGG
                     NPLACAVAIEALKVIVEEKLADNANTMGALFRDELRKLNHPVIELVRGKGLLNAMVIR
                     TKEESEHNWNTDGELAWNICLKMRDNGLLAKPTHGNIIRFAPPLVINESQIKEAVSII
                     KLSIEQSL"
BASE COUNT         1523 a          901 c          782 g         1683 t
ORIGIN      
        1 aattcattcc tattcaactc ttgtaccacg ccaacaacaa tttcatcttt atgttttaat
       61 cctacagaaa taccaaacac tggcacttgg tgaataaaat tagtagtgcc atctaatgga
      121 tcaataatcc actgccattc gccatgcgtt gtggcaatag tgttttcttc tgttaaaaag
      181 gtagcatctt gcataatttt ttgaagctca cgcactaata ttttttcggc ttctttatcg
      241 acataagtca ccaagctatt gagtgctttt gtttcgatat cgctattcga agttttgcct
      301 atttcacttt taataaaagt agctgccttc tctacagcca atattgtttg ttcctgaata
      361 tcgagtaaat gattcattta tttgccatat ttttctttat tgctaattgc aacatgttct
      421 tctttcgttt tcccttccag cacgacaaca aattcgcctt ttggttcttt ttgtgtaaaa
      481 tgaataatcg cttcagcaat gcttcctcta aaattttctt caaatttttt tgtcaattct
      541 ctgctaatag caattcttct gtttccaccg aaataagcat ttagctcttc caataatttt
      601 acaattcgat atggtgattc gtaaaacact attgttcgcg tttcttcttg taatgcctgt
      661 aggcgctttt gccttccttt ctgatgtggt aaaaatcctt cgaaaacaaa tctgtccgtt
      721 ggaaatcctg cattgactaa agcaggtaca aatgcagttg caccaggtaa acattctact
      781 gctatgtcat tttgcaaaca agctctcact agcaaaaatc caggatcaga aattgctggt
      841 gtgccagcgt ctgacaccaa agccatctca acacctgatt taattttttg cacaatagca
      901 tcgactgctt tatgctcatt gtctttatga tatgcttgca atggtgtgtc tattaaaaag
      961 tgttgacata aaaacttaga tgttctagtg tcttctgcta aaatcaattg taccgatttt
     1021 aatgtatcga ttgcacgtaa tgttatatct tgcaagttgc cgataggtgt aggaactata
     1081 tataatttag acatagcgta aagttattga attaccttta tcaataaatc atctaaaaag
     1141 ctaattaaat gaaaaggttt ataagaacgc caatttatag tagcatagcg ctcattgata
     1201 tttatataat ggtgaatatt tgcttgacgc atttctgcta aaacatgttc tctgaaatta
     1261 atggcaataa tccaacctgc attgtctttg atgtcatcgt accattcttc ataatattcg
     1321 ctatcactac taaaaaattc gagtacattt tctccaatta acacgtattt tgaaatgcct
     1381 tgatggagta aaatatctat aaaatctcgt tttaatgttt caatagtatt ttgcgtgcaa
     1441 tcgttccatt ctccaataaa ttcaatgatg gcagcaccag ttttgtaatt tgcatacaac
     1501 aattttaggt atagtgtttc cgcaccaaaa ctgtcccatt gtggatgaat atagtaattg
     1561 taaattgtat ctgaatattc gaactcgctg tactctttgc catagaatgg cgatttttcg
     1621 tcttcttctg ctgtataaaa atcacgccat ttccaatatg gttctatcgt atgcataaca
     1681 agcttgttta ttatactata atttaactag aattagacaa acaatagaaa ttatctaata
     1741 ttacagatta gctgcaagtt ttttttgaat aaaataatca gctacttctt caatcggatt
     1801 gcttacaaat ttgcctaaag tacattcaaa atcattcgct acacgttcta gttctggtcg
     1861 gaaagttttg gcaattgagc ctacaaaacc aattggataa tcttgataat tttcaaaaca
     1921 tgtaatatgg aaatcaaaga attctgtgaa gccttgttgc aacacaactt gcgcataatc
     1981 tgtttctcta aatttagata aaattggtgc aaaactagct aaatatctat ttggcgatgc
     2041 ttctttatag accaattgaa aaatggtatt tttatctaat ttataggctg ataaaatatg
     2101 ctcttctatt tcttgtggca ataaatggta taaaaaatct cgtaatataa tttttccgaa
     2161 gtaagcgcca cctgcttcat cgcctaagat ataaccaata ccagcataag agtccactaa
     2221 attttcgcca tcaaagaaaa tagagtttga gcctgtgcct aatatacatg cgatgccagg
     2281 ctcattgcca caaactgcat aagcagctgc cttcatatca tggtcgattt ctatggaagc
     2341 attataaaaa atacttttta atgcagacgc tgctaattgg ttgcgttcaa cactggagca
     2401 acctgcacca aaaaatttaa tttcagtaat gtcattagca tatgcagtca gctcagtatt
     2461 gctataaatt ttttgataaa tctcttcgtg tttgtgtaca ataggattaa tacctaatgt
     2521 ttcaaaatgt ttcttagtat tatcggcttc attatacaac aaccatgatg ttttagtaga
     2581 gccagaatct gcaaataata tcataattgc aaaaatacgg agtattcagt attcacacaa
     2641 ataatgttta catttgataa aatatttttt tatgaaaact gcattggtga taggcgcaag
     2701 tggattagtt ggtggtcatg tcgtatcgca gctattaaat gagcaagaat ttagtgccat
     2761 aagtatttta acccgaaaac cattgcatct tcaacatcct aaattaaaag aaataatagt
     2821 tgattttgat aatttagaga attatgccac acaaatcaaa gcagattgtg tgttttgttg
     2881 cttaggcaca accataaaaa cggcaggtac gcaagccgca tttcgaaaag ttgattttga
     2941 atatccattg aaagtggccg aaatcgctaa acaaaatggt actacaacat ttatattaat
     3001 ctctgcatta ggtgcttcta aaaactcttt tattttttat aatcgtgtaa aaggagaaat
     3061 tgaggaagca ataagtcaac tacaatttga cagttttcac atcttgcaac caagcttgat
     3121 aattggcgag cgtaaagaca caagactggg agaaggaata gcgcaaaagc tatcaccagt
     3181 ttatgatgtg cttatgtttg gccctttgtc caaatataga tctatcaaag cagaacaaat
     3241 tgcaaaggct atggttgagt atgcaaagtc tgatggcaaa ggtgtatttc gtcacgatag
     3301 cggtgcttta cagcaggtat aattattcta atgtttttta gcattatttt atcttatagt
     3361 agcaattctt aagtgagttt ctaaaaaagg tttaatatca ttccaataat cgccacctaa
     3421 tgctaattcg acggtgtgtg acaaattagg ctgtgctact attgtacacg aatttcctga
     3481 attattaata gctttttgag taggtaacac actatttgtc catgtggctc ctgtagaacc
     3541 atcgttttct tttgcatgaa ataataaaac aggcgaatca ttcgcatcat aatgcaatac
     3601 gccttgctga cttgctgcat catcattgat taaagttgcg cctgtagaaa tcgagccgtt
     3661 tgttttgctc gaaaaaccaa catggtcatt tgtaccaaat ggagcatcat attctatcgc
     3721 atttatcaaa gacaaaccgc cacctgcaga gccaccaaaa acaactatgc gtgttgtatc
     3781 tataaaatat aaatttgcat ttttcttcaa aaatcgaatt ccattttgaa tgtcttcaac
     3841 agcacattgt actgcataca atctcaaaag agaatcttga tttccgccat tctgcgttaa
     3901 tctataattt aaagaagcac aaatataacc tgctctagca taagtgtaag cttcgttttg
     3961 ccaagcttgt ttatcaccac tagagtaacc accaccatga atcatgacca agagtggttg
     4021 ctttgatgtc gagcttgcat ttggtggaag ataaatatcc attttagccg ttaatgtagg
     4081 tttatttatt tctgcctgtt ttgttttctc tgatgtatat tgaagattat gaaaatttgg
     4141 tctggcggta aatgacactt ctaagatgtc aatatctgtt ttttcgtaca ccatactatc
     4201 atacaatgca ggtggcactt gaatgtcatc gtttttttca caagcaatga tacttatggc
     4261 aaaaacagct attagtattc ttatcatatt cctttcgggt tttatgatta gacctaatat
     4321 tgatgaatag gtttaattgc agataaaaaa tcttttttat tataaaataa aataataaaa
     4381 attacagtga ttgctctata gaaagtttaa tgatggaaac tgcttcttta atttggcttt
     4441 cattaatcac taatggtggt gcaaaacgaa taatatttcc gtgtgtaggc ttcgccaata
     4501 aaccattatc tcgcattttt aaacatatat tccacgcaag ttcaccgtca gtattccaat
     4561 tgtgttccga ttcttctttg gtacgaatca ccatagcatt tagcaatcct tttccacgca
     4621 cgagttctat caccggatga tttagttttc ttaattcatc acggaaaagt gcacccattg
     4681 tattggcatt atctgctaat ttctcttcga caattacttt caaagcttca attgccacag
     4741 cacatgctag cggattgcca ccaaatgtag aaccatgttc tcctggtttt atacatagca
     4801 taatttcatc tcgtgccaat acggcactta ccggcatcgt gccgccactt aatgctttgc
     4861 cgagcactaa aatatctggt tttacagac
//
LOCUS       DAOQQV010000152         4853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_8022_length_4853_cov_6.555440, whole genome shotgun sequence.
ACCESSION   DAOQQV010000152 DAOQQV010000000
VERSION     DAOQQV010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8022_length_4853_cov_6.555440"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="PLL59_11565"
     CDS             <1..2181
                     /locus_tag="PLL59_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HMY24491.1"
                     /translation="VTYEVFKAELEFMQNDQKDITDFSNELPNEPFDEGDDDERRSFG
                     GQGTGQPTAKKSASKSNTPVLDNFGRDITKLAEEGRLDPIVGRETEIERVSQILSRRK
                     KNNPILIGEPGVGKTAIVEGLALRIIQRKVSRVLFGKRVVSLDLAALVAGTKYRGQFE
                     ERMKAIMTELEKNRDVILFIDEIHTIVGAGGATGSLDASNIFKPALARGELQCIGAST
                     LDEYRQYIEKDGALDRRFQKVIVDPPSPEETLTILTNLKNKYEDYHSVTYSTEAINAC
                     VQLSTRYITDRFLPDKAIDVMDEVGARVHLKNIHVPENITQLEKEIEDIKEEKNQVVR
                     NQRYEEAARLRDTEKKLQEQLEQAKKDWEEDAKTKRFPVSEEDIAEVVAMMTGIPVKR
                     VAQSESKKLVQMSDDLKKQVIGQDEAVVKITKAIQRNRVGLKDPKKPIGTFIFLGPTG
                     VGKTELARALARYMFDTEDALIRIDMSEYMEKFSISRLIGAPPGYVGYEEGGQLTEKV
                     RRKPYSVILLDEIEKAHPDVYNVLLQVLDDGILTDGLGRKVDFKNSIIIMTSNIGVRQ
                     LKDFGTGVGFATSAIKDNSENLTKGVIQKALQKTFSPEFLNRIDDVIVFNSLGKEEIH
                     KIIDIVLSGVYIRLEKLGYFIELTTAAKDFLSEKGYDPQFGARPLHRAIQKYVEDPLA
                     EEILNANINEGDTIVADLDTEAKDKLKFDIRKKSKIKKTKEEQN"
     gene            complement(2383..3321)
                     /locus_tag="PLL59_11570"
     CDS             complement(2383..3321)
                     /locus_tag="PLL59_11570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMY24492.1"
                     /translation="MQLFNPFDFNDPVAYAVPGFIVLILAEFFIYYRKHHLIDKQFYK
                     DAAASLELGIGSTIIDLAMKAIAISYLLWFYQFRIFDNLGPATIQEFATWQWHREHAY
                     VWLLIFFLQDFIFYWYHRWAHEVRILWAAHVNHHSSEYMHFSTALRQSWLELLVKDLF
                     YIPLAILGFHPLMIITMHQLNLIYQFLPHTESVKRLAKWYEYIFNSPAHHRVHHSSEV
                     RYLDKNYAGILIIWDRIFGTFKEEDKTAFPVYGITTNIHTFNLLKITFHEVQNIWKDV
                     QQAPTIKDKLNYIFQSPGWSHNGKDQRAKTLQKNLK"
     gene            complement(3344..3778)
                     /locus_tag="PLL59_11575"
     CDS             complement(3344..3778)
                     /locus_tag="PLL59_11575"
                     /inference="COORDINATES: protein motif:HMM:NF022703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HMY24493.1"
                     /translation="MKKIIIYFTIVFIGIHSNYGQAFSTPPTVTKEKSTSSKVTKTEK
                     IYKIEQSNVEQEIVLNEQDVLLSGSDNKIHIKGTVANITITGKNNEVDLESVNSILLS
                     SDFNFVSWQTSTNKSGKPIVTDKGGYNNVGKKSANSLQKGEN"
     gene            complement(4061..>4853)
                     /locus_tag="PLL59_11580"
     CDS             complement(4061..>4853)
                     /locus_tag="PLL59_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMY24494.1"
                     /translation="MDFYCTPGKLFAAHYKNLQMENAELQQYVDMLLAWDGVLHPDKI
                     EGSIYKVAKLMTVKKLYGSAIHDNKLIDELLGRGYHAIFGPVNSFLGHNTPTLLRILD
                     DADSFWLKAAGGKEKLLREGFKNAIDWLKLNYGVKTHKWKWGNLHAIVFPHALSVQPP
                     LDKVFDVGPYPIGGDTDTPFQTFIMAAEGYGGELAAPSYRQIIDFSDFDKSTVIMPLG
                     NSGNMASPYYKNQLRDWFEGKDYPMCWTRKKVEEHQKHKLLLKKK"
BASE COUNT         1637 a          858 c          853 g         1505 t
ORIGIN      
        1 gtgacctatg aagtttttaa ggcagaatta gaatttatgc aaaacgacca aaaggacata
       61 acagatttca gcaatgagtt gcctaatgag ccatttgacg aaggcgatga tgacgaacga
      121 cgcagctttg gaggacaagg tacaggccag ccaactgcca aaaagtcggc atctaaatct
      181 aacacacctg tattagataa ttttggaaga gatataacaa aacttgcaga agaaggccga
      241 ttagacccaa tcgtaggtag agaaacagaa attgaacgcg tgtctcaaat tttatcacgc
      301 agaaagaaga ataatccaat tttaataggc gaacctggcg ttggaaaaac agccatcgta
      361 gaaggattgg cattgcgcat cattcaacgc aaagtatcac gtgttttatt tggcaaacgt
      421 gtcgtttctt tagatttagc agccttagta gctggcacaa aatatcgtgg ccaatttgaa
      481 gaacgtatga aggccatcat gactgaattg gaaaaaaacc gtgatgtcat tttatttatc
      541 gatgagatac ataccatcgt tggtgcaggc ggcgcaacag gctccttaga tgcttctaat
      601 attttcaaac cagcattagc acgtggtgag ctccaatgca ttggcgcatc tactttagat
      661 gaatatcgtc aatacattga aaaagatggc gcattggaca gacgttttca aaaagtaatc
      721 gtcgatccac caagtccaga agaaaccttg acaattctta ctaatttaaa aaacaaatac
      781 gaagactacc atagcgttac ctacagcaca gaggcaataa atgcttgcgt acagttgagt
      841 acacgttata tcacagatag atttttgcca gataaagcca ttgatgtaat ggacgaagtt
      901 ggcgcacgtg tgcatttaaa aaacatacat gttccggaga atatcacgca attagaaaaa
      961 gaaattgaag atattaaaga agaaaaaaat caggtcgttc gcaaccaacg atacgaagaa
     1021 gctgctcgcc tacgtgatac tgaaaaaaaa ttgcaagagc aattagagca agcaaaaaaa
     1081 gactgggaag aagatgcaaa aacaaaacgt ttcccagtaa gtgaagaaga cattgcagaa
     1141 gtagtagcaa tgatgacagg cattcccgta aagcgtgtag cacaaagcga aagcaaaaaa
     1201 ttggtgcaaa tgtctgacga cttaaaaaaa caagtgattg gacaagatga ggctgtcgta
     1261 aaaataacaa aagccataca acgcaacaga gttggattaa aagatcccaa aaaaccaatc
     1321 ggcacattta tatttcttgg acctacaggc gttggtaaaa ctgaattagc gcgtgcatta
     1381 gcaagatata tgtttgacac ggaagatgca ttaatcagaa ttgacatgag tgaatacatg
     1441 gaaaaattct ctatctccag attgattggt gcgcctccag gttatgttgg ttatgaagaa
     1501 ggcggacagc taactgagaa agtacgtaga aagccatatt ctgtcatctt attagacgaa
     1561 atagaaaaag cacatcctga tgtttacaat gtattattgc aagtattaga cgatggcatt
     1621 ttgactgatg gcttaggaag aaaagttgat ttcaaaaact ctatcatcat catgacgtca
     1681 aacattggtg tgcgtcaatt aaaagatttt ggtactggtg ttggttttgc tacaagtgcc
     1741 attaaagata actctgaaaa cttaacgaaa ggtgttattc aaaaagcatt gcaaaaaaca
     1801 ttctctccag agttcctcaa tcgtatcgat gatgtgattg tatttaatag cttagggaaa
     1861 gaagaaattc ataaaattat cgacatcgtt ttgtctggcg tttacatacg tttggaaaaa
     1921 cttggctatt ttatcgaact cactactgca gcaaaagatt tcttatcaga gaaaggttat
     1981 gatccacaat ttggtgctcg accattacat cgtgctattc aaaaatatgt agaagatcct
     2041 ttggcggaag aaatactaaa tgctaacatc aacgaaggcg atacaatcgt ggccgactta
     2101 gatacagaag caaaagacaa attaaaattt gacattcgaa aaaaatcgaa aatcaaaaaa
     2161 actaaagaag aacagaatta attttctgtt gaatagtcca aaaaacgcct agaaattcta
     2221 ggcgtttttt tattgatata caaattagtg accattattt atcaaaaata attttattga
     2281 gaatggactg atttttatca aaaaaggcaa gtgaaaattc ctgcttactg cgaataattg
     2341 tgtgattatg taagcacaat tttagtctaa acaaaagtgt atttatttca gatttttttg
     2401 tagtgttttt gctctttggt ctttgccatt gtggctccat ccaggcgatt gaaatatgta
     2461 gttcaacttg tcttttattg ttggtgcttg ctgcacatct ttccatatat tttgcacttc
     2521 atgaaaagta atttttaata aattaaatgt atgaatattg gtggtaatgc catacacggg
     2581 aaatgctgtt ttatcttcct ctttaaatgt accaaaaatt cgatcccaga taattaaaat
     2641 accagcgtaa tttttatcta aatagcgtac ttcactcgaa tgatgtacgc gatggtgcgc
     2701 tggagaatta aaaatatatt cataccattt tgccaatcgc ttaacacttt ctgtatgtgg
     2761 caaaaactga taaattaaat taagttgatg catggtaata atcatcaaag gatgaaaacc
     2821 taatatcgct aatggaatat aaaacaaatc ttttactaat agttctaacc aactttgtct
     2881 caaggcagta gagaaatgca tatattctga tgaatgatga ttaacatgtg ctgcccatag
     2941 tattcgcact tcgtgtgccc aacgatgata ccaataaaag ataaaatctt gcaagaaaaa
     3001 aataagaagc cacacataag catgttcgcg atgccactgc caagtagcaa attcttgaat
     3061 ggtggctgga cctaaattgt caaaaatacg aaattgataa aaccataata aataactaat
     3121 agcaatagcc ttcattgcca aatcaataat ggtagagcca atgcctaact ctaaacttgc
     3181 tgccgcatct ttataaaatt gcttgtcaat gagatgatgt tttctgtagt atataaaaaa
     3241 ctctgcgagt attaaaacga taaaacctgg cactgcataa gctactggat cattaaaatc
     3301 aaatggattg aatagttgca taaattaaga tagatatcga gtactaattt tcaccttttt
     3361 gtaatgaatt tgcagatttt tttcctacat tattatagcc acctttatca gtgacaatag
     3421 gctttccact tttgtttgtt gatgtttgcc aacttacaaa attaaaatca ctactaagta
     3481 gaatcgaatt tactgactcc aaatctactt cattattttt tcctgtaatt gttatgttag
     3541 caactgttcc tttaatgtgt attttattat cactgccaga caataaaaca tcttgttcat
     3601 tgagtacaat ttcttgctcc acattacttt gttcaatttt gtatattttt tctgtttttg
     3661 taaccttaga tgatgtgctt ttttctttag ttacagttgg tggtgtacta aaagcttgtc
     3721 catagtttga atggatacct ataaagacta ttgtaaaata aataatgatt tttttcatcg
     3781 taattttatc ttttttaacg cgttgtgcgg tttgaattta gattgcattt tttatattca
     3841 actgattttc aaaatcaaac ttttgtcact ttttttctta gtcaaaaaaa gtaaccaaaa
     3901 aagacaaggc aaaaatatcg ctccacgcta tagccacgct ttgctggcga tttttgccgt
     3961 cccacgcttc ttatatccta caaattttaa ccagttatac tttttattca ttttttcttt
     4021 tgtaaaaccg caaaatgggt cttttataat aaattagaat ctattttttc ttcaataaca
     4081 atttatgttt ttgatgctct tctacttttt tcctagtcca gcacattgga taatccttac
     4141 cttcaaacca atctctcaat tgatttttat agtatggact tgccatattt cctgaatttc
     4201 ctaatggcat tattactgtt gatttatcaa aatcagagaa gtcaattatt tgcctatatg
     4261 atggtgctgc taactcgcca ccataacctt ctgctgccat aataaaagtt tggaatggcg
     4321 tatctgtatc gccaccaatt ggatatggac cgacatcaaa caccttgtct aatggaggct
     4381 gaacgcttaa tgcgtgtgga aatacgatgg cgtgcaaatt gccccatttc catttgtgtg
     4441 ttttaacacc ataattaagt ttcaaccaat caatcgcatt tttaaaaccc tctctcaaca
     4501 acttctcttt gccacctgct gcttttaacc aaaatgagtc cgcatcatct aagatacgta
     4561 gtaaagttgg cgtgttgtgg ccgagaaaag aattgacagg tccaaaaatt gcatgatagc
     4621 ctctccctaa taattcatct atcaatttat tatcatgaat ggcagagcca tatagttttt
     4681 tcactgtcat cagcttcgcc actttataaa tactcccttc tattttgtct ggatgcaaaa
     4741 cgccgtccca tgccagcagc atatctacgt actgttgcaa ctctgcattt tccatttgca
     4801 aatttttata gtgcgcagcg aatagttttc ctggtgtgca ataaaaatcc att
//
LOCUS       DAOQQV010000153         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_8138_length_4816_cov_7.151649, whole genome shotgun sequence.
ACCESSION   DAOQQV010000153 DAOQQV010000000
VERSION     DAOQQV010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8138_length_4816_cov_7.151649"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3372)
                     /locus_tag="PLL59_11585"
     CDS             complement(<1..3372)
                     /locus_tag="PLL59_11585"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMY24495.1"
                     /translation="MNKISLFIFYTLVVITAYSKTNVAKLVEYVKNEGQWEKQILYKA
                     DLSGAWAFLEKNGITYSLIENSHQHTKNEKHDIVRGHAYQVKWLNSNATSKVYATEKQ
                     AYYNNYFVGNSSNWKSNVGIYRTIHYDNIYPNIDFKIYSQDKAMKSDYIVHTNGNVND
                     IQFQFKGMDKLSIEQNGNLKIKTSIADIYELKPYAFQIINGETKEVPCLYQLSNNVLS
                     FILPNGYNKLYDLTIDPTLVFSSYSGSLSDNWGSSATHDHLGNMFLGGITLGAMYPTT
                     LGAFQTNFGGGSGFQPSDIVITKFNNNGSNKLFSTYLGGSSNELLASLISTPNNELIA
                     VMATSSTDFPTTSNAFDKTFNGGNPALAYEISFPNGADLAITKFNATGTALIGSSFFG
                     GTGNDGTNLNSETAFNYGDESRSDVALDIDGNIYITSTTTSTDIPNTSGKAQANFGGG
                     NTDGLVAKFNADLSVLNWASYFGGSGNDASYSMAIDKNKNIFICGGTNSSDILSANNG
                     LHSNYLGGSTDGFVAKFSNNGNTILSSTYIGTNSYDQSYIIDLDNNDNVYLFGQTLGD
                     YPVSANVYSNDGAKQFIHKLNNALNISLFSTVFGSENSTSINISPTALLVDVCENIYA
                     VGWGGNVNYRGNTFNMALTNDAFKNSTDGSDFYLINLGRDARSLKYATYFGEDGGIGD
                     HVDGGTSRFDKNGIVYQAVCASCGATNAFPTTAGVAGPNNNSDNCNMAGFKFDFNLTG
                     LQIITTTATPHIACAPMNVNFTYTSTQPGTQFFWDFGDGSTSTAEFPAHTYNNAGTYT
                     VKFIIRDPNNCNPVDSSFISVIVKDKKTTSINRSICQGQSVSIGNQTFSTSGTYSITL
                     QAANACDSIVTLHLTVRNSIINNISRDICQGESVIIGTHTYTQTGNYADTFRTNVGCD
                     SIINLQLRVHQPKNSSLQSVLCQGQYIQVGGQTFSETGNYAIHTLTTKGCDSTINLSL
                     QVNPTKETQIVRSICDGEAVTIGNQTFTTTGNHVVTLKTSQNCDSVIQLALSVFPKKI
                     TTLAEEICEGSAYTIGNQAFTTSGVHTVTLQSSNQCDSIVQLTLTVNPMKTTAIKYVV
                     CNGTTITVGTQTFTTSGNYTIPLISAKGCDSTVFLD"
     gene            complement(3469..>4816)
                     /locus_tag="PLL59_11590"
     CDS             complement(3469..>4816)
                     /locus_tag="PLL59_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMY24496.1"
                     /translation="NILHTIQNTYTQKYYIKISNGLCNMVDSIVSIVQSGAPIVQMDD
                     EMQVCADSVRIKSNAQNYDTLFWSTDINFTNIISHSADITISQQAPEVTYYIKLIKQQ
                     CIYIDSIHVLYSDSIPNISLSSSSPFCSDSVFAYAFADFYTEVEWFDDRNLTHLISTT
                     LTLQMTQTIGEKWYYCKVKYKSCVALDSIKITNNAIHYSKPNYEICADSSLTVQLNVQ
                     TPTQYDVLWFINGDTLETNNIATLNISLSQSQIIKFRIDNILGCTIQDSIYVIVHQLP
                     LVEASVDKTSIYIGEEVQLTATNHQQYAYHWQPAESLSNANIYNPVATPIQTTTFIVE
                     VKNENQCRNIDSVNVQVIANECHSSNLFIPNAFSPNGDGVNDILKVKVHSLKSMHLEI
                     YDRWGHKVFESNDINEAWNGTYKSKPAPADTYGYYFIGECIQGEKISKKGNITLLY"
BASE COUNT         1421 a          820 c          872 g         1703 t
ORIGIN      
        1 gtctaaaaat acagtactat cacacccttt agcgcttatt aaaggtattg tataatttcc
       61 agaagttgta aacgtttgtg tgccgacagt aattgtcgtt ccattgcaaa ctacatattt
      121 tatggctgtt gttttcattg gatttacagt caatgtcaat tgtactatgc tatcacattg
      181 attagagctt tgtaaggtaa cagtgtgcac acctgaagtc gtaaaagcct gattgccgat
      241 agtgtaagca ctgccttcgc aaatttcttc tgcaagtgta gtgatttttt taggaaaaac
      301 acttaaggct aattgaatta cgctatcaca attttgtgat gtttttagcg tgactacatg
      361 atttcctgtt gtagtaaaag tttgattgcc aatagttaca gcttctccat cgcaaatact
      421 tcttacgatt tgtgtttctt tagttggatt tacttgtaat gataagttaa tcgtagaatc
      481 acaacctttt gttgtaagtg tgtgaatggc ataatttcct gtttccgaaa atgtttgtcc
      541 acctacttga atatattgcc cttgacacaa tacactttgt agtgagctgt ttttaggttg
      601 gtgtaccctt agttgcaaat tgataatgga atcgcagcca acatttgttc tgaaagtatc
      661 agcataattg cctgtttgtg tataggtgtg tgtgccaatg attacggatt caccttggca
      721 aatatctcta ctgatattgt tgataatact gtttctgaca gtaagatgta aggtaactat
      781 ggagtcacaa gcatttgctg cctgcaaggt aatagaatat gtgccacttg tagaaaatgt
      841 ttgattgccg atgcttacgc tttgtccttg gcaaatacta cgattgatgc ttgttgtttt
      901 tttatctttg acaataacgc taatgaatga ggagtcaaca ggattgcaat tgttgggatc
      961 tcgaatgata aatttaacag tatatgttcc tgcattgttg taagtatgag caggaaattc
     1021 agccgtagaa gtgctgccat ctccaaagtc ccagaaaaac tgagtgcctg gctgtgttga
     1081 tgtgtaagtg aaattgacat tcatcggtgc acaagcgata tgaggtgttg ccgttgtggt
     1141 aatgatttgc aagccagtaa gattaaaatc aaatttaaaa ccagccatat tgcaattgtc
     1201 agaattattg tttggtccag caacgccagc agtagttgga aatgcattgg tagcgccaca
     1261 gcttgcacat actgcctgat atacgattcc atttttatca aatcggctag tgccgccatc
     1321 aacgtgatcg ccgataccac cgtcttctcc gaagtatgtc gcatatttca aagatcttgc
     1381 atcgcgccca agatttatca aataaaaatc acttccatca gttgaatttt taaaagcatc
     1441 attagtaagt gccatgttga atgtatttcc acgataattg acattgccac cccaacctac
     1501 agcataaata ttttcacaaa catcgactaa aagtgcagta ggagaaatat tgatagaagt
     1561 tgaattttcg ctaccaaaaa cagtagagaa taaagaaatg tttaatgcat tatttagttt
     1621 gtgaataaat tgcttagcgc catcattgct atatacattt gcagaaacag gatagtcgcc
     1681 taatgtttgt ccaaataaat acacattatc attattatct aaatcaatga tataactttg
     1741 atcatagctg tttgtgccga tatatgtcga agataaaatg gtgtttccat tattgctaaa
     1801 ttttgctaca aatccgtcag tacttccgcc caaataattt gaatgaagac cattattcgc
     1861 cgataaaata tcgctgctat tggttccgcc acaaataaaa atatttttgt ttttatcgat
     1921 agccatacta tatgacgcat cattgccgga gccaccaaaa taagatgccc aatttaaaac
     1981 agataaatca gcattgaatt ttgctactaa tccatccgta tttccgccac caaaattggc
     2041 ttgcgctttt cctgatgtgt ttggtatatc tgtagatgtt gtggtagagg taatataaat
     2101 atttccatcg atatctagtg cgacatcgct gcgagattca tcaccataat taaatgctgt
     2161 ttctgaattg agattggtac catcatttcc tgtgccaccg aagaagcttg agccgattaa
     2221 tgctgtacct gttgcgttga attttgtaat ggcaaggtca gcaccatttg gaaaactaat
     2281 ttcgtacgca agtgcagggt tgccaccatt gaatgtttta tcaaatgcat ttgatgtagt
     2341 tggaaagtct gtggaacttg tagccattac ggcaatcaac tcattatttg gcgtgcttat
     2401 caaacttgct aaaagctcgt ttgaacttcc tcctaaataa gttgaaaata atttattgct
     2461 tccattgttg ttaaacttag tgataacgat atcgcttggc tgaaatcctg agccaccacc
     2521 aaaattagtt tggaaagcac ctaatgttgt tggatacata gctcccagtg taatgcctcc
     2581 taagaacata tttcctaaat gatcgtgtgt agcagatgag ccccaattgt cggacaaact
     2641 accagaatag ctggaaaata ctaaagttgg atcgatggtt aaatcgtaca atttattata
     2701 gccatttggt aaaataaaag aaaggacatt gttcgatagc tgatacaaac aaggcacttc
     2761 ttttgtttcg ccattgatga tttggaaagc ataaggtttt aattcgtaga tatcagctat
     2821 tgaggtttta atttttaagt tcccattttg ctcaatacta agcttgtcca ttcccttgaa
     2881 ttggaattgt atatcattta cattgccatt cgtatgtaca atatagtcag acttcatcgc
     2941 tttatcttga ctataaattt tgaagtctat gtttggataa atattgtcat aatgtatggt
     3001 tctgtatata ccaacatttg atttccaatt tgatgaattt cctacgaaat aattgttata
     3061 atatgcttgt ttttctgtcg catatacttt tgaagtagca ttgctgttca gccattttac
     3121 ttgataagca tgtcctctga caatatcatg cttttcattt tttgtgtgtt ggtgtgaatt
     3181 ttctattaat gaataagtaa ttccattttt ttcaaggaaa gcccaagcgc cagataggtc
     3241 agctttatat aaaatttgct tttcccattg accttcgttt tttacatatt caacaagctt
     3301 ggctacattg gttttgctat atgcagtaat tacaactaat gtataaaaaa taaacaatga
     3361 aattttattc atacaattat tttcagttgg ataacagaat ttgttttagg tttatgttct
     3421 agcgtttaga gtaatatgct actatgtatc taagttaatc aatatagctt aatataatag
     3481 cgtaatgtta cctttcttac ttattttttc accttgaatg cattcgccaa taaaataata
     3541 accataagta tctgcaggtg caggttttga tttgtatgtg ccattccaag cttcattgat
     3601 gtcattcgat tcaaacactt tgtgtcccca gcgatcatat atttctagat gcatactttt
     3661 caaagaatga acttttactt ttaaaatatc attgactcca tctccatttg gtgaaaaagc
     3721 attaggaata aagaggtttg atgaatggca ttcgttggca attacctgca cattcacgct
     3781 gtctatattg cgacattgat tttcgttttt tacttcaaca ataaaagttg ttgtttgtat
     3841 cggcgttgca acaggattat aaatgtttgc gtttgataat gattcggcag gttgccaatg
     3901 ataggcgtat tgttgatgat ttgttgctgt caactgcact tcctcgccaa tatatattga
     3961 tgttttgtcg acacttgctt ccactaaagg taattgatgc acgataacat aaatgctgtc
     4021 ttgtatggtg caacccaaaa tattatctat tctgaatttt attatttgac tttgagatag
     4081 agagatgttt aatgttgcaa tattatttgt ttctaatgtg tcgccattga taaaccaaag
     4141 cacatcgtat tgcgttggcg tttgcacatt aagttgtaca gtcaacgagc tatctgcaca
     4201 aatttcgtaa tttggttttg aataatgtat ggcgttattt gtaattttta tactatcaag
     4261 agcaacacac gatttatatt ttaccttaca atagtaccat ttttcgccta tggtttgagt
     4321 catttgtaat gttagtgtag tgcttattaa atgtgtcaga tttctatcgt caaaccattc
     4381 aacttccgtg tagaaatctg cgaaagcata agcaaaaaca gagtctgaac aaaaaggaga
     4441 tgatgacgac agacttatgt tgggaatgct gtcgctatat aacacatgaa tgctgtcaat
     4501 gtatatgcat tgctgtttta ttagtttaat ataatacgtt acttcaggtg cttgctgtga
     4561 aatagtaata tctgctgagt ggctaataat atttgtaaag tttatgtccg ttgaccaaaa
     4621 taatgtatca tagttttgag cattgctttt aattcgaaca ctgtctgcgc agacttgcat
     4681 ttcatcgtcc atttgtacta tcggcgctcc tgattgtact atgcttacga tgctgtccac
     4741 catattacat aagccattac ttatcttgat ataatacttt tgagtgtatg tattttgtat
     4801 agtatgtaga atattt
//
LOCUS       DAOQQV010000154         4714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_8399_length_4714_cov_7.600343, whole genome shotgun sequence.
ACCESSION   DAOQQV010000154 DAOQQV010000000
VERSION     DAOQQV010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8399_length_4714_cov_7.600343"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PLL59_11595"
     CDS             complement(<1..1475)
                     /locus_tag="PLL59_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HMY24497.1"
                     /translation="MKFNSNIVALFLTLFSLQTLKAQSFNAKPETWYVDAEKVPCDYY
                     VNECFLVKQPGQADYEVYGDKIEGFEYEAGYKYTIVVKQTIKEPPIAAGESVFKYVLV
                     KVVSKKSIAPIKTVVAPAVSNNNAGDKTKIFEINFETVPCDAPDTKACLLIKEKGKTE
                     YEIFYGSIYGFNYQEGYNYTISVKETSNGNYYFVNEISKKLIKAQLLQNAALNSNNNN
                     TTGVTKTQSGKIIQPTSIQTDSKLDGKWYLRKMKENDVSSIVTDDNVIWLDINTFNDR
                     IDGYGSCNKFSAVVRSDLNTTFLVSKITAGGINCGNKKIENLLFQLLEQCNRFELKNG
                     NLILSDQWNFLLGFTKNADSKEDISSTYTPPSIVKNDVTKYASSQSASSSNDGYIPPS
                     ANTIVTESNSTSYTTSTYSNTSVPAPTKIDYEKIEKEKAAALALQLEEEKKAEEIKLK
                     KIAELKAKQEAELAALQQQLDDKKQKVAAELKAQAELKLKQ"
     gene            complement(1549..3858)
                     /gene="pbpC"
                     /locus_tag="PLL59_11600"
     CDS             complement(1549..3858)
                     /gene="pbpC"
                     /locus_tag="PLL59_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HMY24498.1"
                     /translation="MQKFYSFLARHQFKFLSLCCLLMLFNVVDLLFPLPTQKDYSTII
                     EAENGYILSAFLTHDDKWRMYTQLDEITPTVQKAFIHKEDKWFYWHFGVNPIAIVRAL
                     YNNTIAQRRTSGASTITMQVARMLEPKERNMLSKIKEIARALQLEWNYSKDEILQLYL
                     NLVPYGSNIEGVKSASYLYFGKLPNQLSLAEVTTLTIIPNRPTSLQLGKKNNAIQEVR
                     NRCLLKFKKNKVFSETEIDDALEEPLTAYRRTPPKIAAHFCIRLKKQFQSIPIIPTYI
                     NYNMQLDVEEMVNNYSKILQFKDIKNAAVFIVDNQNHQVISYVGSPDFTSVSTQGQVD
                     GVKAIRSPGSTLKPLIYGLAFDKGIYTPRSIITDVPIDFDGYTPENYDEHFHGKVALA
                     DALAQSLNIPAVKVLNDIELNTLINTLDKIDFKQIEKDKDKLGLSTALGGCGVSLEEL
                     VGMYSIFANNGTFEAPKMTALTKSNQAIEILSPQANYMVAQILTTLRRSDLPNRAESV
                     INLPKIAWKTGTSYGRRDAWSIGFNNKYTVGVWCGNFDGKGVPELNGADIATPLLLQI
                     FNLIAKNNDRDWFKRPKDLQFRKVCAESGDIPNEDCTDLIYAYYIPGKSGNKKCTHLK
                     KVWVSMDSTITYCSSCVGNNNYIEKWYPNYAPELIAFYESKNLPYQKTPEHNTNCVKI
                     RESNYPTITKPVNGLTYYIHSTNQKLMLSAQAANDVEQLAWYVDNKFIRSAPKNEKIF
                     IDASLGKIKISCVDDKGRNTDAWVIVKQI"
     gene            complement(3943..>4714)
                     /locus_tag="PLL59_11605"
     CDS             complement(3943..>4714)
                     /locus_tag="PLL59_11605"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HMY24499.1"
                     /translation="ENIVKLAIEGGCNAVASTYGVLGSVARKYAHRIPFIVKINHNEF
                     LSYPNSFDQIMFGTIKDAWNMGAVAVGATIYFGSPESKRQIIEVSKAFEYAHELGMAT
                     ILWCYTRNNDFKKDGVDYHTATDLSSQANHLGVTIQADIIKQKLPTLNGGYNALKFGK
                     THPKVYSELTTDHPIDLTRYQVANCYMGRNGLINSGGESKGQTDLAEAVETAIINKRA
                     GGMGLISGRKAFQKEVKVGVELLNAIQDVYLDNEVSIA"
BASE COUNT         1378 a          823 c          743 g         1770 t
ORIGIN      
        1 tcttgtttaa gttttagttc tgcttgtgct tttagttctg cggccacctt ttgtttcttg
       61 tcatctaatt gctgttgtaa tgccgctaat tctgcttcct gttttgcttt aagttctgct
      121 atttttttta attttatttc ttctgccttc ttttcttctt ctaactgcaa ggctaatgca
      181 gctgcttttt ctttttctat tttttcataa tcaatctttg ttggcgcagg tactgaagtg
      241 ttgctatacg tagatgttgt gtagcttgtt gaattgcttt ctgtaactat tgtattggca
      301 cttggtggaa tatatccatc attcgatgaa gatgcacttt gactcgaagc atattttgtg
      361 acatcatttt ttacaatgct tggcggtgta taagtagatg aaatatcttc tttgctgtca
      421 gcattttttg taaaacctaa taagaaattc cattggtctg ataaaattaa attgccattt
      481 ttaagctcaa atctattgca ttgttctagc aattgaaaaa gcaagttttc aattttctta
      541 ttgccacagt taatgccacc agcagtaatt ttgctcacta aaaaggttgt gtttaaatca
      601 gaacgcacca cagcagaaaa tttattacat gagccataac catcaattct gtcgttaaat
      661 gtattgatat ctaaccatat cacattatca tcagttacaa tgcttgacac atcgttttct
      721 ttcattttgc gtaagtacca tttgccatct aatttagaat cggtctgtat gcttgttggc
      781 tgaatgattt taccactttg tgttttcgta actccagtag tattattatt gttgctattt
      841 aatgcagcat tttgaaggag ttgtgcttta attaattttt tagaaatttc atttacaaaa
      901 tagtaatttc cattactggt ttcttttaca cttatcgtat agttgtagcc ttcttgataa
      961 ttaaagccat atatagaacc ataaaatatt tcatattctg tttttccttt ttctttgatt
     1021 aataaacaag cctttgtgtc tggcgcatcg cacggtactg tttcaaagtt tatttcgaat
     1081 attttagttt tatcaccagc attattattt gatacagctg gcgcaactac tgtttttatg
     1141 ggtgctattg attttttaga aacaactttt actaacacat atttaaaaac agattctcct
     1201 gcagctattg gtggttcttt tattgtttgt tttacaacta tcgtgtattt gtaaccagcc
     1261 tcatattcga agccttcgat tttatcgcca tatacttcgt aatcggcttg accaggttgc
     1321 ttaaccaaaa aacattcatt gacatagtaa tcacaaggca ctttttcagc atctacatac
     1381 catgtttctg gtttagcatt aaaagattgt gcttttaagg tttgtaatga aaatagcgtt
     1441 aaaaatagtg ctacaatgtt ggaattaaat ttcatagtta atatttatca actaaaatac
     1501 aataattaaa tattttatca aaatatgaca taccaaaata aagtattttc atatttgctt
     1561 aacgatgacc caagcgtcag tatttctgcc tttgtcgtcc acacaagata ttttaatttt
     1621 tccaagtgat gcatcgatga atattttttc attttttgga gcacttcgta taaatttatt
     1681 gtcaacgtac catgcaagct gttcaacatc atttgccgct tgtgccgaca acattaattt
     1741 ttgatttgtc gaatgaatat aataggtgag tccgtttacg ggttttgtta tggtaggata
     1801 atttgattct cttattttca cacagtttgt attgtgttca ggcgtttttt gataaggcag
     1861 gtttttggat tcgtaaaagg caatcagttc aggcgcataa ttaggatacc atttttcaat
     1921 ataattatta ttacctacac atgatgaaca gtatgtaata gtgctatcca tgctcaccca
     1981 aacttttttc aaatgcgtac attttttgtt gccacttttt cctggaatat aataggcata
     2041 aatcaaatct gtacaatctt cattaggtat atcgccactt tctgcgcata cttttctaaa
     2101 ctgtaaatcc tttggccgtt taaaccaatc tctgtcattg ttttttgcta ttaaattaaa
     2161 tatctgtaat aataatggag tagctatatc tgcgccattt aattcaggaa cgcctttgcc
     2221 atcaaaattt ccacaccaaa caccaaccgt atatttatta ttaaagccaa tgctccaagc
     2281 atctcgtctg ccatatgaag tgcccgtttt ccaagctatc tttggtaggt ttatcacgct
     2341 ttcagctcta tttggcaaat cgcttctccg caatgttgtt aatatttgtg ctaccatata
     2401 attagcctgt ggagaaagaa tttcaatagc ttgattgctt tttgttaatg ctgtcatttt
     2461 gggcgcttca aacgtaccgt tgttagcaaa aatactatac ataccaacta gttcttctaa
     2521 ggaaacacca caaccaccta atgcggttga taaacctaat ttgtctttat ctttttctat
     2581 ttgtttgaag tcaattttat ctaaagtatt gattaaagta tttaattcaa tgtcatttaa
     2641 tactttaacc gcaggaatat ttagtgattg agcaagtgca tctgccaagg ctactttacc
     2701 atgaaaatgt tcgtcataat tttctggtgt atagccatca aaatcgatag gaacatctgt
     2761 aattattgaa cgtggcgtat aaataccttt gtcaaatgca agtccataaa ttaagggctt
     2821 gagtgtgctg cctggagaac gtattgcctt tacaccatca acttgtcctt gagtggaaac
     2881 acttgtaaaa tcaggcgaac caacatagct tatcacttga tgattttgat tgtcaacaat
     2941 aaatacagca gcatttttga tgtctttaaa ttgcaatatt ttagaataat tgttgaccat
     3001 ttcctctaca tctaattgca tattgtaatt aatataagta ggaataattg gtattgattg
     3061 aaactgcttt ttcaatcgaa tacaaaaatg tgcagctatt tttggtggtg ttcttcgata
     3121 tgccgttaaa ggttcttcga gtgcatcatc aatttctgtt tcggaaaaaa ctttattctt
     3181 tttgaatttt aataaacatc gatttcttac ttcttgaatg gcattatttt ttttacctaa
     3241 ttgtaatgat gttggcctgt tgggaataat tgttaatgta gtcacttcag ccaaactcaa
     3301 ctgatttggc aatttaccaa aatataaata tgatgcagat ttgacgcctt ctatattgct
     3361 gccataaggc actaaattaa gatacaactg taatatttcg tctttagagt aattccattc
     3421 gagttgtaat gcacgtgcta tttcttttat ttttgaaagc atatttcttt ccttaggttc
     3481 caacatacgt gccacctgca ttgtgatagt cgatgcacct gaagtccgcc gttgtgctat
     3541 tgtgttatta tataatgcac gtacaatggc aattggattt acaccaaaat gccagtagaa
     3601 ccacttatct tctttatgta taaatgcttt ttgcacggtt ggtgttattt cgtctaactg
     3661 tgtgtacatt cgccatttat catcatgcgt tagaaatgca gataagatgt agccgttttc
     3721 tgcttcaata attgttgaat aatctttttg tgttggaagt ggaaataata aatctactac
     3781 attaaaaagc attaacaagc agcacaagct taaaaacttg aactgatggc gtgctaaaaa
     3841 tgaatagaat ttttgcatga tgaaaaagaa taacacaaaa tactaaataa aaaaatgctc
     3901 caatagctgg agcattttaa taactatttt tagtttaaat aattaagcaa ttgacacctc
     3961 attatctaaa tatacatctt gaatggcatt taataattca acgcctactt ttacctcttt
     4021 ttggaatgcc tttctgccag aaatcaagcc catgccacca gctcgtttgt tgatgatggc
     4081 agtttctact gcttccgcta agtccgtttg tcctttactc tcaccaccag aattgattaa
     4141 accatttctg cccatatagc aattagctac ttgatagcga gttaagtcga ttggatgatc
     4201 tgtagttaat tcagaatata cttttggatg tgttttgcca aattttaaag cattatatcc
     4261 gccatttaaa gttggtaact tttgtttaat gatatccgct tgtatggtta cacctaagtg
     4321 attcgcttgt gatgataagt cagtagcagt gtggtaatca acaccatctt ttttgaaatc
     4381 attgttacgt gtgtagcacc ataatatagt tgccatgcct aattcatgtg catattcaaa
     4441 tgcttttgac acttcaataa tttgacgttt tgattcagga gaaccaaaat aaatagtagc
     4501 acctacagca acagcaccca tattccaagc atctttgata gtgccaaaca taatttggtc
     4561 aaatgaattt ggatatgaca aaaattcgtt gtgatttatt tttacgataa aaggaattct
     4621 atgtgcatat tttcttgcaa cagaacctaa aacgccataa gtagaagcaa cagcattaca
     4681 gccaccttca attgctaatt ttacaatatt ctca
//
LOCUS       DAOQQV010000155         4611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_8677_length_4611_cov_7.514486, whole genome shotgun sequence.
ACCESSION   DAOQQV010000155 DAOQQV010000000
VERSION     DAOQQV010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8677_length_4611_cov_7.514486"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /gene="hppD"
                     /locus_tag="PLL59_11610"
     CDS             <1..895
                     /gene="hppD"
                     /locus_tag="PLL59_11610"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HMY24500.1"
                     /translation="INTHLVKHGDGVKAIALWVEDATKSFEETTKRGAKPFMQPTIEK
                     DDFGEVVRSGIYTYGETVHIFVERKNYKGIFLPGYQAWNSDYNPSVVGLKYIDHMVGN
                     VGWNEMNTWVKWYEDVMGFVNFLSFDDKQIHTEYSALMSKVMSNGNGRIKFPINEPAE
                     GKKKSQIEEYLDFYESAGVQHIAVATDDIITTVSALRARGVEFLSAPPHAYYEEIPAR
                     LGEHMRIMKEDISVLEKLAILVDADEDGYLLQIFTKPVEDRPTLFFEIIQRMGAKGFG
                     AGNFKALFESIEREQEKRGTL"
     gene            906..1325
                     /locus_tag="PLL59_11615"
     CDS             906..1325
                     /locus_tag="PLL59_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMY24501.1"
                     /translation="MNFNYGFILESTAKKIKLELQRKFNEHEVDITVDQWVVLFELHQ
                     HGTQNQVSLCEHCAKDAPTITRIIELLVKKELIVREACTNDRRKYNISLSKKGKTLIQ
                     RLLPIVIEFRIQGWLNLNEKDIMHIERITKKIQDNLS"
     gene            1373..2593
                     /gene="fahA"
                     /locus_tag="PLL59_11620"
     CDS             1373..2593
                     /gene="fahA"
                     /locus_tag="PLL59_11620"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HMY24502.1"
                     /translation="MTIQELSNNNEHFTIYNLPYGIFSTPTKSKRVGIAIGNYIIDLS
                     VLYDLNFFEGRIQNNVFSNETLNNFIALGKEIWQFTRKKIYTLISEESDFFNAHHTSF
                     VYQQCSAVMHLPVNIGDYTDFYSSEEHASNVGKMFRPNMEPLLPNWKQMPIAYHGRSS
                     SIITTKTPVRRPYGQVLKGEEVIYSASQTLDIEIELGFIIGKENQLGTPININDATDY
                     IFGAVLLNDFSARDIQRWEYQPLGPFLGKNFATSISPWIVPFEALKEFMTDAPTQTQT
                     VLPYLQEAHRKNFDIEISFAIETKNKNKAVLAVSNAKYLYWTAEQQIAHHTVNGCNLR
                     IGDLLGTGTISGKEKNSFGSLLELTWNGKEKLMVGTEERTFLQDGDTTILSAICKNEN
                     KQIGFGEVSNLIIG"
     gene            2632..3408
                     /gene="phhA"
                     /locus_tag="PLL59_11625"
     CDS             2632..3408
                     /gene="phhA"
                     /locus_tag="PLL59_11625"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HMY24503.1"
                     /translation="MYQLKQAYDKYTAEDFLVWKLLYQRQRVLLQNMASLEFIEGMEA
                     MHFSDEKIPDFNHVNKVLANTTGWQIEVVPGLIPDKDFFELLANKKFPSSTWLRKLEN
                     LDYTEEPDMFHDCFAHMPLLTEQFFVDYLQALSKIALRYIDDALAIELIGRIYWFSVE
                     FGLIQQNNGMRIYGAGILSSSGESIYCLGDKANRLPFNVKEIMEKHYYKDHFQDRYYV
                     INTYKDLFDAIPEIDTLLERALKQIPHEKNINATPLDLKL"
     gene            3411..3581
                     /locus_tag="PLL59_11630"
     CDS             3411..3581
                     /locus_tag="PLL59_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24504.1"
                     /translation="MKKQTLKQIKSSVRKKQIKDGFFDGRFMPKTQDNKKKVQNKKQC
                     RIKIVLHNDNIN"
     gene            3611..4477
                     /locus_tag="PLL59_11635"
     CDS             3611..4477
                     /locus_tag="PLL59_11635"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMY24505.1"
                     /translation="MKTIIPSKIGLKEISNFITSSVVPRPIAFVSTVNANGQANLSPF
                     SFFNAFSSDPPILIFSAAKSALSGKQKDTYHNVKAIPECVIHIANFAMVEQLSVTSGL
                     YPSDVNEFVKGGFTEEKSLMVKAPRIKEAPIAFECKVNQVIELGDKGGSGNLIVCEIL
                     CIHVDENLLDEKGNVIAENLDPIARLGYNNYVRFTKDDIFETPKPLKAYGVGVDAIPQ
                     WIKDENVLTGNELGKLASIAEIPNKEDIQEDAELVELISDYKSQSINEIKRLLENNKI
                     KEAWLVLLNLNK"
BASE COUNT         1705 a          725 c          867 g         1314 t
ORIGIN      
        1 aataaacacg catttagtca aacatggtga tggtgtaaaa gccattgctc tttgggttga
       61 agacgctaca aaatcttttg aagaaacaac aaaacgcggc gcaaaaccat ttatgcaacc
      121 aacaattgaa aaagatgatt ttggtgaagt cgtacgctca ggcatttata cttatggcga
      181 aactgtacat atttttgtgg agcgaaaaaa ttataaaggt atattcttac caggctatca
      241 agcttggaat tctgattaca atccaagcgt tgttggatta aaatacatcg accacatggt
      301 aggcaatgta ggctggaatg aaatgaatac ttgggtaaaa tggtatgaag atgtaatggg
      361 ctttgtcaat tttttgtcat ttgatgataa acaaatccac acagaatatt ctgccttgat
      421 gagcaaggtg atgagcaatg gaaatggcag aataaaattc ccaatcaatg agcctgctga
      481 aggcaaaaag aaatcacaaa tcgaagagta tcttgatttt tatgagagtg caggcgtgca
      541 gcatattgcc gtagccactg atgacatcat tacaacggta agtgctttac gtgcacgtgg
      601 cgtagaattt ttaagtgcac caccacatgc ttattatgaa gaaattcctg cccgattagg
      661 tgagcacatg cgcatcatga aagaagatat cagcgtattg gaaaagttgg caatattagt
      721 agatgctgat gaagacggct acttattaca aattttcact aagcctgtgg aagacaggcc
      781 aacactgttt tttgaaatca ttcagcgcat gggtgcaaaa ggatttggtg ccggaaattt
      841 taaagcatta tttgaatcta tagaacgaga acaagaaaaa agaggaacat tataaataaa
      901 acaaaatgaa ttttaattac ggatttatat tagaatctac agcaaaaaaa ataaaattag
      961 aacttcaacg taagttcaac gaacacgagg tggatattac cgttgaccaa tgggttgttc
     1021 tttttgaatt acatcaacat ggcactcaaa atcaagtgtc gctttgtgaa cattgtgcca
     1081 aagatgcacc aacgataacg cgcatcatcg aactattagt aaaaaaagaa ttaatagtaa
     1141 gagaagcatg tactaatgat cgacggaaat ataatatttc attgagtaaa aaagggaaaa
     1201 cattaataca aagactattg cctatcgtga ttgaatttcg aatacaaggt tggcttaatt
     1261 tgaatgaaaa agacatcatg catatagaaa gaataacaaa aaagatacaa gacaacttaa
     1321 gttaattatt aaattaaaac acattaaaat ttagtgctta aaataaataa atatgacaat
     1381 ccaagagctc agtaataata acgaacattt taccatatac aatttgccgt atggtatctt
     1441 ttctacgcca acaaaatcta agcgtgtggg cattgctatc ggaaattata ttattgactt
     1501 gtctgtttta tatgacttga atttttttga aggcagaatt caaaataatg ttttcagtaa
     1561 tgagacttta aataatttca tcgctttagg caaagaaata tggcaattca caaggaaaaa
     1621 aatttatacg ctcatcagtg aagagtctga tttttttaat gcacatcata cttcatttgt
     1681 atatcagcaa tgtagtgctg tgatgcatct accagtaaac attggcgatt acaccgattt
     1741 ctattcatca gaggaacatg ctagcaatgt cggaaaaatg tttagaccaa acatggagcc
     1801 actacttccc aactggaaac aaatgcctat agcctatcat ggtcgaagct catcgatcat
     1861 tactacaaaa acaccggtaa gacgaccgta tggtcaagtg ctgaaaggcg aagaggtaat
     1921 ttattctgca tcgcaaacac tagatataga aatcgaatta ggatttatca tcgggaaaga
     1981 aaaccagctt ggaacaccaa taaacattaa tgatgccaca gattatattt ttggtgctgt
     2041 tttattaaac gacttttctg ctcgcgatat tcaacgttgg gaatatcaac ctttaggacc
     2101 atttttaggg aaaaactttg caacgagtat ttcaccttgg attgttccat tcgaagcgct
     2161 aaaagaattt atgacagatg cacctacaca aacacaaacg gtgttaccat atttacaaga
     2221 agcgcatagg aaaaatttcg atatagaaat cagctttgcc attgaaacaa aaaacaaaaa
     2281 caaagctgtc cttgctgtaa gcaatgctaa atatttatat tggactgctg agcaacaaat
     2341 cgcacaccat acggtgaatg gttgtaattt gcgcatcggt gatttattag gcactggcac
     2401 tatttctgga aaagaaaaaa atagttttgg aagcttatta gaattaactt ggaacggaaa
     2461 agaaaaatta atggtaggaa ctgaagagcg aactttttta caagatggcg acactaccat
     2521 attaagcgca atttgtaaaa atgaaaataa acaaattggt tttggcgaag tatcaaattt
     2581 gataataggc tgaatataat ttaaaataaa taatcgattt agcacttaca catgtaccaa
     2641 ttaaaacaag catacgacaa atacacagct gaagattttt tggtatggaa attattgtat
     2701 cagcgacaac gggtgctctt gcaaaacatg gcttctcttg aatttatcga aggcatggaa
     2761 gcgatgcatt ttagcgatga aaaaatcccg gattttaatc atgtcaataa agttttagcc
     2821 aatacaactg gctggcaaat cgaagtggtg ccaggcttaa ttcctgataa agattttttt
     2881 gaattattgg caaataaaaa atttccatca tctacgtggt tgcgcaaatt ggaaaattta
     2941 gattacacag aagaaccaga tatgtttcac gattgctttg cgcacatgcc attgctgaca
     3001 gagcagttct ttgtcgatta tttacaagcg ctttcaaaga ttgctttgcg ttatatcgat
     3061 gatgctttag caattgagct gattggtcga atttactggt ttagcgtgga gtttggtttg
     3121 atacaacaaa ataatggtat gcgtatttat ggtgctggta ttttatcttc aagtggcgaa
     3181 agtatttatt gtttaggtga caaagccaat cgtttgccat ttaatgtgaa agaaattatg
     3241 gaaaaacact attacaaaga ccacttccaa gatagatatt atgtcatcaa tacctacaaa
     3301 gatttatttg atgcaattcc tgagattgat actttgttgg aacgagcctt aaaacaaata
     3361 ccacatgaaa aaaatataaa tgcaacacca ttagatttaa aattataaaa atgaaaaagc
     3421 aaaccttaaa acaaataaaa tctagtgtgc gaaagaagca aattaaagac ggctttttcg
     3481 atggtagatt tatgccaaaa acacaagaca ataaaaagaa agtgcaaaat aaaaaacaat
     3541 gcagaataaa aattgtacta cataatgaca atatcaacta aggtacatct cgacaaataa
     3601 actcctaaaa atgaaaacaa tcataccaag caaaatcggt ttaaaagaaa tatcaaattt
     3661 cattacgtca tcagtcgtgc cacggccaat cgcatttgtg agcacggtga atgctaatgg
     3721 tcaggctaac ttaagtccat tttcattttt caatgcgttt agttccgatc cgccgatatt
     3781 gattttttca gcagcaaaaa gtgcacttag cggcaaacaa aaagacactt atcataacgt
     3841 aaaagctatt cctgaatgcg tgatacatat tgccaacttc gctatggtgg aacaattatc
     3901 cgttacaagt ggtttgtatc caagcgatgt aaatgaattt gtaaaaggtg gtttcacaga
     3961 agaaaaatct ttgatggtaa aagcgccacg catcaaagaa gcgcctattg catttgaatg
     4021 taaagtaaat caagtaatag aattaggcga caaaggtggt tctggaaatt taatcgtttg
     4081 tgaaattcta tgtattcatg tcgatgaaaa tttgttagac gaaaaaggta atgttatcgc
     4141 agaaaatctt gatccaattg cacgcttagg ttataataat tatgtacgat ttaccaaaga
     4201 cgatattttt gaaacaccta aacctttgaa agcttatggc gttggcgttg atgccattcc
     4261 acaatggata aaagatgaaa atgtattgac tggaaatgag ttaggaaaac tggcaagcat
     4321 agcagaaatt ccgaacaaag aagacatcca agaagatgca gaattagtag aattaatttc
     4381 tgattataag agccaaagta ttaatgaaat aaagcgtttg ctcgaaaata ataaaataaa
     4441 agaagcgtgg ttggtgttgt tgaatcttaa taaataaaaa aagtgccaca ttaaaacatg
     4501 gcactttcat ttgtatttta aggcaatgtg gtcaaaaaac cattctatcg tggttagtga
     4561 ggacaacgaa ccacggcaaa aagaatagga ttaacctgga cctggtttta t
//
LOCUS       DAOQQV010000156         4608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_8691_length_4608_cov_6.875247, whole genome shotgun sequence.
ACCESSION   DAOQQV010000156 DAOQQV010000000
VERSION     DAOQQV010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8691_length_4608_cov_6.875247"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /gene="mnmA"
                     /locus_tag="PLL59_11640"
     CDS             <1..951
                     /gene="mnmA"
                     /locus_tag="PLL59_11640"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HMY24506.1"
                     /translation="DYMFAEYEAGRTPNPDVLCNREIKFDIFLKHALALGADFVATGH
                     YCRKRVSTDEHGNPRYHLLAGKDGNKDQSYFLCQLNQAQLAKALFPIGELLKPEVRAI
                     AKAQNLVTAEKKDSQGLCFIGKVRLPDFLQQQLQSKKGEVILIDDNSLSPTAKYTFDN
                     CTTTQFQQLAEAFRFKKGDGKIIGEHNGAHFYTIGQRKGIKIGGFKEPLFVIQTDTKE
                     NLLFLGEGEQHKGLNRAGLFIKKEEVHFIREDLKLKAGEKSIYAARIRYRQALEKCCL
                     YQTEQGLYVIFENLQKGIAPGQFCAWYQDDELIGSGVIAY"
     gene            complement(1017..1250)
                     /locus_tag="PLL59_11645"
     CDS             complement(1017..1250)
                     /locus_tag="PLL59_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24507.1"
                     /translation="MAKMTKVFGAIFCVLGTIYLTVSIFLYFNTNAHLDYNEAKQMSF
                     IHSGTPFYFIVSILILGFGIVFAKDKVLDNDHH"
     gene            complement(1331..2572)
                     /locus_tag="PLL59_11650"
     CDS             complement(1331..2572)
                     /locus_tag="PLL59_11650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMY24508.1"
                     /translation="MDNVDSLIIENKNPKPTHNEYQIVFLIAFIGFLASTDSMLLMPL
                     SNIIMAKYDVSAQVSTWVISTYSIFAGIAGFLSASIMDRFDRKYLLIGTFLGFSITTL
                     LCGFADNFGLFVFYRGITGIFGGVVGGVGVAIVSDLIPFDRRATAMSIISLSFAVSAI
                     IGVPIGIHFASEYNLSVPFKILAFISFLTLIPIFLWLPKIDKHIKEHIEHTSPFKTIK
                     LAVKDSNQVLALLLSILIVFSHFVVLQFIVPYMENNVGFSSKLVPVMYFIGGICAIAT
                     APFIGKLSDRKGNFPSFILLMILSFVPIILSTHIRVVSMPLALFICATFFILAAGRMI
                     PAYTIMSGATTPEKRGGFMSMRSACMELGTALASIVSGFVVKIGADGLVHHFNYVGYI
                     SVATGLICIFIASKIKIVSHE"
     gene            complement(2628..3785)
                     /locus_tag="PLL59_11655"
     CDS             complement(2628..3785)
                     /locus_tag="PLL59_11655"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HMY24509.1"
                     /translation="MIAHSLLRDAFKTQFGYFASAYYFSPGRVNLIGEHIDYNGGYVM
                     PAAISLGISAAVAWRDDDIICVYANDFKEKSTFSISDNLADINKTAIHWSDYIKATLQ
                     VLKDKNTKLRGADIYISSDLPLGAGLSSSAALECLIAFIFDEVESEKNRTALALDAQL
                     AERKYVGVNCGIMDQFAVANGKDKHAIILNCATLDYEYTPADFKSYQLVIINSNKSRT
                     LASSKYNERRKECDAAFEQLKKIDIAADLCHIHEISLATLEDDVLYQRAKHAILENKR
                     VKHAVQVLKNGNIEAFGQLLTESHISLDRDYEVSCHELNMIVHYSTHFEACIGARMTG
                     AGFGGCCIALVAKEKVPQFIAYVGKKYTEKTGLKADFYCAEIVDGVRSILE"
     gene            complement(3853..4416)
                     /locus_tag="PLL59_11660"
     CDS             complement(3853..4416)
                     /locus_tag="PLL59_11660"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HMY24510.1"
                     /translation="MKYVLVALLACTMLFESKAQLTVNGVSLPAKLKTATSELDLNGG
                     GVRKKAFFKVYVLGLYLPEKSKDAAAILKNNQEIAVRLQITSSVVSSSNMSEAIREGF
                     TKSLGGNTAPMKAKIDAFISIFSKEEIKEKDVFILDYVPGVGVKSFKNGKLLSTTEGE
                     DFKKALFGIWLGPNPIDTGLKTAILGN"
BASE COUNT         1598 a          835 c          779 g         1396 t
ORIGIN      
        1 gattatatgt ttgcagaata cgaagctgga agaacaccaa atcctgatgt attgtgtaat
       61 cgagaaataa aatttgatat tttccttaaa catgcactcg ctttaggtgc tgattttgtg
      121 gcaactggac actattgccg caaacgagta agcactgacg aacatggcaa tccaagatat
      181 cacctattgg caggaaaaga tggcaataaa gatcaaagtt attttttgtg tcaattaaat
      241 caagcgcaat tagcaaaagc tttatttcca attggcgaat tattaaaacc tgaagtaaga
      301 gccatagcaa aagctcaaaa tttggtgaca gcagaaaaga aagactcgca aggtctttgt
      361 tttattggca aagtgcgcct accagatttt ttacaacaac aattacaatc aaaaaaaggc
      421 gaagtcatat taattgatga taatagctta tcgcctacgg caaaatatac ttttgacaat
      481 tgcacaacaa cccaatttca gcaacttgcg gaagctttcc gtttcaaaaa aggtgatggc
      541 aaaataattg gcgaacacaa tggtgctcat ttttatacaa taggtcaacg taaaggcatc
      601 aagataggtg gttttaaaga gcctctattt gtaatacaaa cagacaccaa agaaaattta
      661 ctatttcttg gcgaaggtga gcaacacaaa ggtttaaatc gcgctggttt atttattaaa
      721 aaggaagagg tacattttat aagagaagac ttgaagttaa aagctggcga aaaaagcata
      781 tacgcagctc gcattagata tcgccaagca ttagaaaaat gttgtttata ccaaacagaa
      841 caaggtttat acgtaatttt tgaaaaccta caaaaaggca ttgcacctgg gcaattttgc
      901 gcttggtatc aagatgatga actaataggc agcggtgtta ttgcctatta aaaaataaac
      961 ctcggttata aataaccgag ggcaaaaact tataaccatg aaaacatccg ataatcttaa
     1021 tgatgatcgt tatctaatac tttatctttt gcaaatacga ttccaaagcc tagtatcaaa
     1081 attgatacga tgaaatagaa aggtgttcct gagtggataa atgacatttg ttttgcttca
     1141 ttataatcta aatgagcatt tgtgttgaag tacaagaaaa ttgatactgt tagatatatc
     1201 gtacccaata cacaaaaaat tgcgccgaat acttttgtca tttttgccat aatgtgattt
     1261 tttttacaaa tttaagcatt ctatactatt ttcgtataaa aactatcaac ataaaaataa
     1321 attaacactg ctactcatgg ctgacaattt taatcttaga agcgatgaaa atgcaaatca
     1381 atcctgttgc cacagaaatg tagccaacat agttaaaatg gtggacaaga ccgtcggctc
     1441 caattttcac aacaaaaccg ctcacaattg atgctaatgc cgtgcctaat tccatacagg
     1501 cagatcgcat actcataaaa ccacctcgtt tttcaggtgt ggtggcgccg ctcattatag
     1561 tatatgcagg aatcatacgg ccggctgcca atataaaaaa tgtggcgcaa ataaataatg
     1621 ccaatggcat gctcaccaca cgaatgtgtg tactcagaat aatgggaaca aatgacaata
     1681 tcatcaaaag aataaaggaa ggaaagtttc cttttcggtc agacaattta ccgatgaatg
     1741 gtgctgtagc gatggcgcaa atgccaccaa taaaatacat aacaggaact aacttactgc
     1801 taaatccaac attattttcc atgtaaggca cgataaactg caacacaaca aaatgactga
     1861 atacaattaa aatagataag agcaaggcca aaacttgatt gctatctttt acggcaagtt
     1921 ttattgtttt aaaaggactt gtgtgttcta tatgttcttt tatatgttta tcaatctttg
     1981 gcaaccacaa gaagatagga ataagcgtaa gaaaagaaat aaatgctaaa attttgaatg
     2041 gcacagataa gttatattca ctagcaaaat gaatacctat aggcacacca atgatggcag
     2101 aaacggcaaa tgacaatgaa attatactca tggctgttgc tctgcggtca aacggaatta
     2161 aatcgctaac gatagctacg ccaacaccac caactacacc accaaaaatt ccggtgattc
     2221 ctctataaaa tacaaacaag ccaaaattat ctgcgaagcc acacaataat gttgtaatag
     2281 aaaatcctag aaaggtacca ataagtaaat atttcctgtc aaacctatcc atgatagatg
     2341 cagacaaaaa accggcaata cctgcaaaaa tagaataagt agaaattacc catgttgaca
     2401 cttgagcaga cacatcatat ttagccatga taatattgct taatggcatc aacaacatag
     2461 aatctgtaga tgctaaaaaa cctataaatg caattaaaaa cacaatctga tattcgttgt
     2521 gcgttggttt tggattctta ttctcaatta ttaaactatc tacattatcc attacgcttg
     2581 tttatacttg gtgatgaata gctttatata tcaaaaacga taaacggcta ttctaatatg
     2641 ctacgcacgc catctacgat ttctgcgcaa taaaaatcgg ctttaaggcc tgttttctct
     2701 gtatattttt tgccaacata agcaataaat tgtggtactt tttcttttgc taccaaggca
     2761 atacaacaac cgccaaaacc tgcacctgtc atacgagcgc cgatacaagc ttcgaagtgt
     2821 gtactgtaat gtacaatcat attgagctca tgacagctga cttcataatc gcgatccaag
     2881 gaaatatgcg attcggtaag caattgtcca aatgcttcaa tattgccatt tttaagcact
     2941 tgcacggcgt gtttcactct tttattttcc aagatggcat gttttgcacg ctgatataaa
     3001 acatcatctt ctaaagtagc taatgatatt tcatgtatat gacataagtc tgctgcaatg
     3061 tcaatttttt tcaattgttc aaacgcagca tcacattcct ttctgcgttc attatattta
     3121 gatgaggcta aggttcttga tttattggaa ttaataatca ccaattgata cgatttaaaa
     3181 tctgctggtg tatattcata atcgagcgtt gcacaattca agatgatggc gtgtttgtct
     3241 ttgccatttg ccacagcaaa ctgatccatg atgccacagt ttacgccaac atatttacgc
     3301 tctgctaact gcgcatctaa tgctaatgct gttctatttt tttcgctttc aacttcatca
     3361 aaaataaatg ctattaaaca ttctaatgct gctgacgagg aaagcccagc tcctaatggc
     3421 aaatcgctgc taatataaat atcggcaccg cgaagttttg tgtttttatc ttttagcact
     3481 tgcaatgtcg ctttgatata atcagaccaa tgtatggctg tcttattgat atcagccaaa
     3541 ttatctgaaa tggaaaaagt tgatttttct ttgaaatcat ttgcataaac acaaataata
     3601 tcatcatcgc gccatgcaac tgcggcagaa atacctaaag aaatagcggc tggcataaca
     3661 tagccaccgt tatagtcgat atgttcgcca atgagattta ctcttccagg cgaaaaataa
     3721 tatgcagatg caaagtagcc gaattgcgtc ttaaacgcat cacgaagtaa agaatgagca
     3781 atcataagtt aggaggcaaa tttaaagaaa gtatctttct tatttatacc tacaaatttg
     3841 taattggaaa aattaattac ctaaaattgc agttttcaaa cctgtatcta ttggattagg
     3901 tcctaaccaa attccaaata atgctttttt gaaatcttca ccttctgttg tcgataataa
     3961 tttaccgttt ttaaatgact tcacaccgac acctggaaca taatctaaaa tgaaaacgtc
     4021 tttttctttt atttcttctt tagagaatat agaaataaag gcatcgattt ttgctttcat
     4081 tggagctgtg ttgccaccta atgatttagt aaaaccttcg cgaatagctt cagacatatt
     4141 gctactgcta acgacacttg atgttatttg taaacgcaca gcaatttcct gattattttt
     4201 caagatggca gcagcatctt tgcttttttc aggcaaatat aaacccaata cgtaaacttt
     4261 aaagaatgcc tttttacgaa cgccaccacc atttaaatct aattctgatg ttgcggtttt
     4321 cagttttgct ggtaagctta cgccattcac tgttaattgt gctttgcttt cgaatagcat
     4381 ggtgcatgct aataatgcta ctaatacgta tttcatgttt taattttaat tgttgaatat
     4441 actacaattt gataaaagag caaaagaaat gccaaactta tcgatggagt gaatagtttg
     4501 ttgtatagga gaaaactaaa aaaaattgga aactattatt tcgctttggt tgatgaggac
     4561 accataagtg ttcggaagat tacgttgtat tataagaata gagaaatt
//
LOCUS       DAOQQV010000157         4334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_9504_length_4334_cov_7.744333, whole genome shotgun sequence.
ACCESSION   DAOQQV010000157 DAOQQV010000000
VERSION     DAOQQV010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9504_length_4334_cov_7.744333"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1939)
                     /gene="typA"
                     /locus_tag="PLL59_11665"
     CDS             complement(140..1939)
                     /gene="typA"
                     /locus_tag="PLL59_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HMY24511.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKILWECKTFAEHENTGVLILDN
                     NELERERGITITAKNAAVKYNDYKINLIDTPGHADFGGEVERVLKMADGVLLLVDAFE
                     GPMPQTRFVLQKALQLGLKPIVVINKVDKENCTPDEVHEKVFDLMFQLDATEEQLDFP
                     TIYGSAKNGWMSTDYKKPTTNVVDLLDCIIEHIPAPKTAEGAAQMQVTSLDYSSFVGR
                     IAIGRVFRGSLKTNMPVALIKRDGAIVKSRIKELLLFEGMNKIKAEEVPAGEICAIVG
                     VEGFEIGDTIADLEQPEAMAPISIDEPTMSMLFTINDSPFFGKEGKFVTSRHIKDRLT
                     KELEKNLALRVQQGDTADSFLVFGRGVLHLSVLIETMRREGYELQVGQPQVLYKEIDG
                     TKCEPMEELTIDLPEEKAGTAIEMITKRKGDMKNMILKGNRSIMEFEIPSRGLIGLRT
                     DMITATSGQAVMAHRFTHYAPHKGEIQGRINGSMISKETGEAIAYSLDKLQDRGRFFV
                     EPGDEIYTGMVIGENSRQDDLVVNCTVTKKLTNVRASGTDDKVKLPPAVKFSLEEALE
                     YIQGDEYVELTPKSIRLRKIYLDENERKRKGGK"
     gene            2128..>4334
                     /gene="uvrA"
                     /locus_tag="PLL59_11670"
     CDS             2128..>4334
                     /gene="uvrA"
                     /locus_tag="PLL59_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMY24512.1"
                     /translation="MSDNSLPPISQEEITHINVYGAKEHNLKNIDVHIPRNQFIVITG
                     LSGSGKSSLAFDTIFAEGQRRYMESFGAYARQFLGNMERPNVEKISGLSPVISIEQKT
                     TNRNPRSTVGTTTEIYDFLRLLYARIGEAYSYNTGEKMVRFSEEQIIEDILHKFQDKK
                     IAILAPLVRGRKGHYRELFEDIRKQGYLRVRVDGETQEIQAKMQVDRYKIHDIEVVID
                     RIKTPKNKHSSEQTSADMQRLRESINKALKEGKGLLFIEDLESGKVATYSKTLMCTTT
                     GISYEEPSPNTFSFNSPYGSCPTCKGLGVIKTADYSKVIPDDTKSILEQGIAPLGEYR
                     NTYTYKIVEQILKQFKYTLKTPIRDLSEDCLSVLLYGTEEKFKIEAKENPARKSSNTH
                     WSLSFDGVINQINKSFTETSSDGLRNWAENYISEVNCSECNGTRLKKDSLWFKLDNKN
                     IAEIASYDLNELDAWINQLDKKLTKNQNIIAKDIIKELQDRIGFLLNVGLNYLHLNRA
                     TASLSGGESQRIRLATQIGSQLQGITYILDEPSIGLHQRDNQRLILALRNLTEIGNTV
                     IVVEHDKDIMLAADYILDLGPKAGAHGGEVVAQGTPKEFLKLSATTAKYLSNKIAIEI
                     PAHRRSGNGKTIKLIGAKGNNLKNVTLEIPLGTFTCISGVSGSGKSTLINETLYPILN
                     QYLYRSPFNPMPYTAIEGIENIDKVIEIDQSPIGRTPRSNPATYIGVFDKIRE"
BASE COUNT         1378 a          885 c          742 g         1329 t
ORIGIN      
        1 cgccattatt gacttgaagc actattcttt gctccatttt tgaccacatt gtctacataa
       61 aacataaaaa aacctgtcaa gtgcttcact taacaggttt ataaatcatg ctaatcaaaa
      121 aatcagctac agctgtgttc tattttccac ctttgcgctt gcgctcattt tcatctaaat
      181 agattttacg caaacgaata gattttggtg tcaactctac atattcatcg ccttgtatgt
      241 attccaatgc ttcctctaaa gagaatttca cggctggtgg caatttcact ttatcatcag
      301 taccagaagc acgtacgttt gttagttttt tagtaactgt acaattcacc actaaatcgt
      361 cttgacgaga gttttcgcca atcaccattc ctgtataaat ttcgtcgcct ggctctacaa
      421 agaaacgacc tctatcttgc aatttatcta aagaataagc aatcgcctca ccagtttctt
      481 tagaaatcat agaaccattg atacgtcctt gaatttcccc tttatgcggc gcataatggg
      541 tgaatcgatg tgccatcact gcttgtcctg atgttgccgt aatcatatcc gtacgcaaac
      601 ctattaaacc acgtgaaggt atttcaaatt ccatgataga acgatttcct ttcaaaatca
      661 tgtttttcat gtcgcctttg cgctttgtta tcatttctat tgccgttcct gctttctcct
      721 ctggcaagtc gatagttaat tcttccatcg gctcacattt tgtaccgtcg atttctttgt
      781 ataatacttg tggctgacct acttgcaact catagccttc acggcgcatc gtctcaatca
      841 atacagacaa gtgcaataca ccacgaccaa acaccaagaa agaatctgcc gtatcacctt
      901 gttgcacacg taaggctaag tttttttcta actctttagt caatctatct ttgatatggc
      961 gcgatgttac aaatttgcct tctttgccaa aaaatggtga gtcgttaatg gtaaacaaca
     1021 tgctcattgt tggctcgtca atcgatattg gtgccattgc ttctggttgt tccaaatcgg
     1081 caatagtatc gccaatttca aaaccttcta cacctacaat agcacaaatt tcaccagcag
     1141 gcacttcttc tgccttaatt ttattcatac cttcgaacaa caatagctct ttgattcgcg
     1201 attttacaat agcaccatct cgtttaatta aagctactgg catatttgtt tttagtgagc
     1261 ctctaaacac acgaccaata gcaatacgac ctacaaatga agaataatct aatgacgtta
     1321 cttgcatctg tgccgcacct tctgctgttt ttggcgctgg tatatgctcg atgatacaat
     1381 ctaataaatc gacaacgttg gtcgttggct ttttataatc tgtactcatc cagccatttt
     1441 tagcagaacc ataaatagtc ggaaaatcca attgttcttc tgtcgcatct aattggaaca
     1501 tcaaatcaaa tactttctcg tgcacctcat ctggtgtgca gttttcttta tctactttat
     1561 taataactac aattggtttt aagcctaatt gtaaagcttt ttgtaataca aatcgtgttt
     1621 gtggcattgg tccttcaaaa gcatctacta gcaataaaac gccatcagcc atttttaata
     1681 cacgctctac ttcgccgcca aaatctgcgt gacctggtgt gtcaatcaag ttaatcttat
     1741 agtcgttgta ttttacagct gcatttttcg ccgtaatcgt aataccacgt tctcgctcca
     1801 actcgttatt gtctaaaatt aagactcctg tattttcatg ctctgcaaat gttttgcatt
     1861 cccataaaat tttatctact aaagtcgtct taccgtggtc aacgtgtgcg ataatcgcaa
     1921 tatttctgat tttttgcata gaatgatttg tgcttaaaaa tgaggtgcaa aggtacgctt
     1981 ttgtgcacgt tttttatgca tatcgttaaa tgcgtagcat tttttttact ttacctttat
     2041 gagttttaac cttatcataa tgtgttattt atcatttagg cctcaaactc aaaaatcttt
     2101 cttaagttta tagttcattt tttacaaatg tcagacaatt ccttaccacc aatttcacaa
     2161 gaagaaatta cacacataaa tgtatatggt gcaaaggagc ataatttaaa aaatatagat
     2221 gtacatattc cacgcaatca atttatcgtc ataactggcc taagtggaag cggaaaatca
     2281 tcattggcat tcgatactat tttcgctgaa gggcaacgtc ggtatatgga aagtttcggt
     2341 gcctatgcac gtcaatttct cggcaacatg gagcgtccaa acgtagaaaa aatatctggt
     2401 ttgtcgcctg ttatttctat agaacaaaaa acgaccaata gaaatccgag aagtacagta
     2461 ggtacgacaa cagaaattta tgattttcta cgcttgttat atgcgcgcat cggcgaggcc
     2521 tattcgtaca acacaggcga aaaaatggtg cgcttttctg aagaacaaat tattgaagat
     2581 atactccaca aatttcaaga taaaaaaata gctatcttgg cgcctttggt tcgtggcaga
     2641 aaagggcact atcgcgagtt atttgaagac atcagaaaac aaggttattt gcgtgtgcgt
     2701 gtcgatggcg aaacgcaaga aatacaagcc aaaatgcaag tcgatcgcta caaaattcac
     2761 gacattgaag tggtgattga tagaattaaa acaccaaaaa acaagcatag tagcgaacaa
     2821 acatcggctg atatgcaacg cttgcgcgag tctatcaata aagcattaaa agaaggaaaa
     2881 ggtttgctat ttattgaaga tttagagagc ggaaaagtag ctacttatag caaaaccttg
     2941 atgtgtacca ctacaggcat ttcttatgaa gagccatcgc caaatacatt ttccttcaac
     3001 tcaccgtatg gctcttgtcc aacttgcaaa ggactcggcg ttattaaaac tgctgattat
     3061 agcaaggtaa ttccagatga tacaaagagc atcttagagc aaggcattgc acctttgggc
     3121 gagtatcgaa atacatacac ctacaaaatt gtagaacaaa ttttaaagca attcaaatac
     3181 actttaaaaa cgccaatacg cgatttatct gaagactgct tatccgtatt attgtatggc
     3241 acagaagaaa aatttaagat agaagctaaa gaaaatccag cgcgtaaatc atcaaataca
     3301 cattggagtt tatcttttga tggcgtcatt aatcaaatca ataaatcatt cacagaaact
     3361 tctagcgatg gcctgcgcaa ctgggctgaa aattatatca gcgaagtaaa ttgtagcgaa
     3421 tgcaatggca ctcgtctgaa aaaagattct ttatggttca aactcgacaa taaaaacatc
     3481 gccgaaatcg catcgtatga tttgaatgaa ttagatgctt ggataaatca actcgacaaa
     3541 aaattgacaa aaaatcaaaa tatcatcgct aaagatatca tcaaagaatt gcaagacaga
     3601 ataggttttt tattgaatgt tggtttaaat tatttacatc tcaatcgagc tacggcaagc
     3661 ttatcaggtg gcgaatcaca gcgcattcga ttggccacac aaattggctc gcaattacaa
     3721 ggcatcactt atatcttaga cgaaccgagt atcggattgc atcaacgcga taatcaacga
     3781 ttaattttag cattgcgcaa tcttacagaa attggcaaca cagtaatcgt agtcgaacac
     3841 gataaagaca tcatgcttgc ggctgattat attttagatt taggcccaaa agctggcgca
     3901 catggcggcg aagtagtagc gcaaggaaca ccaaaggaat ttctaaaatt aagcgcgact
     3961 actgctaagt atttatcgaa taaaattgcc attgaaattc ctgcacatcg aagatcagga
     4021 aatggcaaaa ccatcaagtt aattggtgca aaaggcaaca acttaaaaaa tgtaacatta
     4081 gaaattccct taggcacatt tacttgtatt agcggtgttt ctggctctgg aaaatctact
     4141 ttaataaatg agacattata tccaatttta aatcaatatt tatatcgttc accatttaat
     4201 ccgatgccat acacagctat tgaaggcatt gaaaacattg ataaagtcat cgaaatagac
     4261 cagtctccaa ttgggcgcac gccacgcagc aatcctgcaa cttatattgg cgttttcgac
     4321 aaaataagag agtt
//
LOCUS       DAOQQV010000158         4299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_9610_length_4299_cov_6.585533, whole genome shotgun sequence.
ACCESSION   DAOQQV010000158 DAOQQV010000000
VERSION     DAOQQV010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9610_length_4299_cov_6.585533"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLL59_11675"
     CDS             complement(<1..577)
                     /locus_tag="PLL59_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMY24513.1"
                     /translation="MSIIQVIIVAIIEGLTEFLPISSTAHMIFTSTIFFGTEKNEFVK
                     MFQVSIQFGAILAVVFLYWKKFFDFKNIQFYFKLAAAVVPALVLGKLFDDKIEAVLEK
                     PIPIAIVLIIGGIVLLFVDKIFTQAQIKNEKDITFKTAIIIGFWQCLAMMPGTSRSAA
                     SIIGGMQQKLDRKTAAEFSFFLAVPTMLAVTM"
     gene            complement(633..992)
                     /locus_tag="PLL59_11680"
     CDS             complement(633..992)
                     /locus_tag="PLL59_11680"
                     /inference="COORDINATES: protein motif:HMM:NF022741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HMY24514.1"
                     /translation="MAKNQAPKNTNTPKQPQATQARNTQKAKSSSGTFLRLPDSQNKT
                     LLFDKTNYILIALGAALIIVGFILMSGGNTDPNVFNEAEIYSFRRITLAPITVIAGFL
                     VIVVAILKKPAGDSEIV"
     gene            complement(1003..1878)
                     /locus_tag="PLL59_11685"
     CDS             complement(1003..1878)
                     /locus_tag="PLL59_11685"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HMY24515.1"
                     /translation="MSADLERKTPKKSKPNFIYAIVSIAMILFTIGLFSFGVFVVQKE
                     INKIKEGVQIDLNLKTDVTEAQKEDIAKYLKKQKYVAKITFKSKEVAAEQFEKELGQN
                     FREILGENPLFDAYIINLNADYSNPDFVKDVKSALVGLSGVQEVFYSDMAVKTTAATL
                     KPITIGIVLLSIVMLIVAFFIIDNTIRLMMYSQRFTIRSMQLIGANEWFIIKPYIVKS
                     IISGVISAGIAIITLSGIIYLTIYKFSVVFTGQDFVTLTIIAICLVIFGIIISITSTY
                     FAVNKYLKIKLDELY"
     gene            1957..2625
                     /locus_tag="PLL59_11690"
     CDS             1957..2625
                     /locus_tag="PLL59_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HMY24516.1"
                     /translation="MNNDNLGLELYLQATFFVDSDQPDIQRFVKNIIGAEIDKQKIAI
                     KLYNAVRDGWRYNPYNIDLKHKAMKASSLFNRETKRGYCIEKACLLNACLRVAGIPSR
                     FRFFDVKNHIGVEKLIQILKTDVLVFHACCEIYLNGKWIKATPAFNKELCEHLGVAPL
                     EFDGENDSIFQQYDTHGGLFMDYLKDYGTFHDVPHDMFIMLLHQHYAHFFNQVTDRAM
                     VNVD"
     gene            complement(2894..3352)
                     /locus_tag="PLL59_11695"
     CDS             complement(2894..3352)
                     /locus_tag="PLL59_11695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMY24517.1"
                     /translation="MKLNYKLFLLLLPFIAFACKKESVDQAAIDREIIRKYIADNSIN
                     ADSTESGLFYAISDSGTAPNPTATSAVLVYYKGYFTDGVRFDSNDPGLPREFSLAGVI
                     KGWQEGIPKIGKNGKIKLMIPSALGYGASEFGSIPANSVLIFDIELDSFR"
     gene            complement(3367..>4299)
                     /locus_tag="PLL59_11700"
     CDS             complement(3367..>4299)
                     /locus_tag="PLL59_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMY24518.1"
                     /translation="TAISGDCDYKVKDDKEALDTIKKLVNKYGKFEMAGFDRIKSKKP
                     KAAEKDIYGIFSTTKPYDVREIIKRLVDDSEFVEYKEDYGQTIVCANARIDGWSVGIV
                     ANQRSVVKSKKGEVQIGGVIYSDSADKAARFVMLCNQKKIPLVFLQDVTGFMVGSRSE
                     HGGIIKDGAKMVNAVSNSTVPKFTIITGNSYGAGNYAMCGKAYDPRLIIAWPSAKIAV
                     MGADQAAKTILQMQVGSLEAKNKTELPEEEKQKILSEIKERYTKQTSAYYAASRLWID
                     AVIDPLETRKWISKGVEAANNAPIKKFNVGVFQV"
BASE COUNT         1335 a          736 c          705 g         1523 t
ORIGIN      
        1 acatcgttac ggcaagcata gtaggtactg ctaagaaaaa tgaaaattca gcagctgttt
       61 ttctatctaa tttttgttgc attcctccaa tgattgaagc ggcacttctg cttgttccgg
      121 gcatcatggc taagcattgc caaaagccaa taattattgc tgttttgaaa gtaatgtctt
      181 tctcgttttt tatctgtgct tgcgtaaata ttttatcgac gaataataaa acgataccac
      241 caataattaa tacaattgca attggaatag gtttttctaa tacagcttca attttatcgt
      301 cgaatagttt tcctaaaaca agtgcaggta ctactgctgc agctagtttg aaataaaatt
      361 gtatgttttt aaagtcaaaa aacttcttcc aatataaaaa aacaacagct aagatggcac
      421 caaattgtat ggaaacttga aacattttta caaattcatt tttttctgtg ccaaaaaaaa
      481 ttgtgcttgt aaatatcatg tgtgctgttg acgaaatagg taagaattca gttaatcctt
      541 ctataattgc aacaataatt acttgtatta tggacattga gaatgtgcta ttgaaatatt
      601 aaatttcgga atgtagaatg aaaaattatc ttctatacta tttctgaatc accagcaggt
      661 ttttttaaga tggctaccac aatcaccaaa aaacctgcaa ttacggtaat aggtgccaaa
      721 gttattctgc ggaaagaata gatttcagct tcattaaata catttggatc cgtattgcca
      781 ccactcatca atataaaacc tacaatgatt aatgctgcac caagtgcaat taatatataa
      841 ttagttttgt cgaataataa ggttttattt tgactgtcag gcaatcgcaa aaatgtacca
      901 gaagaacttt tcgctttctg cgtattacgt gcctgcgttg cttgtggttg cttaggtgtg
      961 tttgtatttt taggagcttg atttttagcc atgtttattt atttaataca attcgtcgag
     1021 tttgattttt aaatatttat ttacagcaaa gtatgtactc gtgatagaaa ttattatacc
     1081 aaaaatcacc agacatattg caataattgt caaagttaca aaatcctgac ctgtaaatac
     1141 aacggaaaac ttataaattg ttaaatagat aattccgcta agggtaataa tggcaatacc
     1201 tgcactgata acgccagaaa taatagattt tacgatgtat ggtttgatga tgaaccattc
     1261 attagcacca atgagttgca tacttctaat ggtgaaacgt tgcgaataca tcataagtcg
     1321 aatggtgttg tcgataataa aaaatgcaac aatcaacatg acgatactca ataaaacaat
     1381 gccaatggta attggtttta aagtggctgc cgttgttttt acggccatat cagaataaaa
     1441 tacttcttgt acgcctgata aaccaaccag cgctgatttt acatctttca cgaaatcagg
     1501 attagaataa tcggcattca gattaatgat atatgcatca aacaaagggt tttcaccgag
     1561 tatttctctg aagttttgtc caagttcttt ttcgaattgc tcagccgcta cttctttact
     1621 tttaaaagta atttttgcca catatttttg ttttttcaaa tatttggcaa tgtcttcttt
     1681 ttgcgcttca gtaacatcag tttttagatt caaatcaatt tgaacacctt cttttatctt
     1741 gtttatttct ttttggacga caaatacgcc gaaggaaaac aagccaattg taaataatat
     1801 catagcaata cttacaatag cgtaaatgaa attaggtttg cttttttttg gtgttttgcg
     1861 ttctaaatct gctgacatat tggcgaaaat aatgattttt tatttactta atctattacg
     1921 ttattaacaa ggtgtgcgat tgcctatttt tacaccatga acaacgataa tttaggtttg
     1981 gaattgtatc tacaagcaac attctttgta gatagcgatc agccagacat acaacgtttt
     2041 gtaaaaaaca ttataggcgc tgaaatagat aaacaaaaaa ttgctataaa gctttataat
     2101 gcagttcgtg atggctggcg ttataatcca tataatatag accttaagca taaggccatg
     2161 aaggcaagct ctttgtttaa tagagaaact aaacgtggtt attgtattga aaaagcgtgt
     2221 ttactcaatg cctgtttacg tgttgctggc attccaagtc gatttcgctt ttttgatgta
     2281 aaaaatcata ttggtgtaga gaaattaata caaatcctaa aaacagatgt actcgttttt
     2341 catgcatgct gtgaaattta tctaaatgga aaatggataa aagcaacgcc agcttttaat
     2401 aaagaattat gtgagcattt aggtgtagcg cctctagaat ttgatggtga aaatgattct
     2461 atttttcagc agtatgacac acacggcggt ttgtttatgg attacctaaa agactatggc
     2521 actttccatg atgtgcctca cgatatgttt attatgctgt tacatcaaca ctatgcacat
     2581 ttttttaatc aagttacaga tagagcaatg gtaaatgtag attgaatttt tttatgataa
     2641 ttcacttatt taaaccattg aaaaacgcat ataatagcta aacaattcaa ttattttttc
     2701 ccatttttca aaaaaaaata atggatatga ttttaatcat actcattgtg tttttttatg
     2761 ttgaattttg tagtacaatg atttacgaaa tcaaagtgtt gtattgaatt aacttaatac
     2821 atccgttggg aaatggacgt agttttatca tagctttttt agaagacggc attcctgccg
     2881 tcttttttta tttttatcta aagctgtcta attcgatatc aaaaatgagc acggaattcg
     2941 ctggaataga gccgaattca ctggcaccgt agcctaaagc agatggtatc attagtttta
     3001 ttttgccgtt ttttccaatt tttggaatgc cttcttgcca gccttttata acaccagcca
     3061 aagagaattc acgaggcaat ccagggtcgt tgctgtcaaa tcgaacacca tcagtaaaat
     3121 aacctttgta atatactaaa acagcagaag tagcagtagg attaggcgca gtgcctgaat
     3181 cacttatggc ataaaacaaa ccagactcag tagaatcagc attgatggaa ttgtctgcaa
     3241 tatattttct aataatttct cgatcaatag cagcttgatc aacactttct tttttacatg
     3301 caaatgcgat aaatggtagt aagagtagga ataacttgta atttaatttc attttttgtt
     3361 tcttttttaa acttgaaata cgccaacatt aaattttttt attggtgcat tgttggctgc
     3421 ttctacacct tttgaaatcc attttcttgt ttctaatgga tcgataacag catctatcca
     3481 caaccttgaa gcagcataat acgctgatgt ttgtttggta taacgttctt ttatttcaga
     3541 tagtattttt tgcttttctt cttccggcaa ttctgtttta ttttttgcct caagtgagcc
     3601 tacttgcatt tgcaaaattg tttttgctgc ttgatcggcg cccatcactg ctatttttgc
     3661 gcttggccat gctattatta atcttgggtc gtatgcctta ccacacatgg cataattgcc
     3721 tgcgccataa gaattaccag ttattattgt aaattttggt actgtagaat tggaaacagc
     3781 attcaccatc tttgcgccat ctttaattat gccgccgtgt tcgcttctac tgccaaccat
     3841 aaaacctgtg acatcttgca agaatactaa tggtattttt ttctgattgc agagcataac
     3901 aaagcgcgcg gctttatctg ccgaatcaga atagatgaca ccaccaattt gtacttcgcc
     3961 ttttttactt ttcacgacgg aacgttggtt ggcaactatg cctacactcc agccgtcaat
     4021 acgtgcattt gcgcaaacaa tggtttgtcc gtaatcttct ttatattcaa caaactcaga
     4081 gtcgtccact aatcttttta tgatttcgcg aacatcataa ggttttgtcg tggaaaaaat
     4141 tccatatata tctttctcgg ctgcttttgg tttttttgat tttattctat caaaacccgc
     4201 catctcaaat ttgccatatt tattaactaa ttttttgatg gtatctaatg cttctttatc
     4261 gtccttgact ttataatcac aatcaccact aattgcagt
//
LOCUS       DAOQQV010000159         4205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_9946_length_4205_cov_5.176386, whole genome shotgun sequence.
ACCESSION   DAOQQV010000159 DAOQQV010000000
VERSION     DAOQQV010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9946_length_4205_cov_5.176386"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /gene="sucC"
                     /locus_tag="PLL59_11705"
     CDS             complement(<1..1116)
                     /gene="sucC"
                     /locus_tag="PLL59_11705"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HMY24519.1"
                     /translation="MNLHEYQGKELLKQYGVKVQEGIVAETKEQAIDAWKVISARTGS
                     EGVVVKAQVHAGGRGKGGGVKFAPNLEALKEKVDQILGMTLVTPQTGAQGKVVHKILI
                     AEDAYYKGASETKEFYMSVLLDRKKECNVIMYSTEGGMDIEEVAEHTPEKIFKEWIDP
                     TIGLQAFQARQIAFNLGLSGNAFKEMVDFTQRLYKAYEGSDSSMFEINPVLKTSDDKI
                     IAVDAKVNLDENGLFRHPDYAAMRDVLEEDPTEVEAGEHNLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLAGGDPANFLDVGGTANAARVEQAFRIILKDPNVKAILVNIFGGIVRC
                     DRVAQGIVDAYNNIGEIPVPIIVRLQGTNADEAKKLID"
     gene            1311..2114
                     /locus_tag="PLL59_11710"
     CDS             1311..2114
                     /locus_tag="PLL59_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HMY24520.1"
                     /translation="MLKKSQHLELLVHLHPYCFSYAIIDKTQHQLIDFEVKVINQQLG
                     KFLHLDTIALWFADHEETFSLPFKTSKVAIYSPEFTILPEKTDKTAELFKLLGYPNSD
                     NITYLKNKISDTFYVYYSLPDKTINFIENHLPNVEFFCIDYGLLNFYNSKLSFKNYVA
                     ANLYGEELTICYKRNNENLYYNKFPVKGKEDLLYYLRLTYEHLELSLNEFPTYLYGFI
                     EEKSPMYSTTYGYIRNFEIDRTLKNTLSLRMTIEDIPLHYYINLIGLDL"
     gene            2111..2677
                     /locus_tag="PLL59_11715"
     CDS             2111..2677
                     /locus_tag="PLL59_11715"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HMY24521.1"
                     /translation="MRIISGTLKGKRFYPPKNIPARPTTDFAKEALFNILNNHFDFSD
                     IKFLDLFAGTGSLDMEMFSRGCTDITSVDMSKISLGFIKKMSEEVKIPNHKIIFGDAI
                     QFAKNCTQQYDLIFAGPPYALEIIDEIPDIIFDKKMLKENGMFILETSPKHDFTEHSK
                     LLEVRNYGQTHFWFFGDETNDDDDEEDE"
     gene            2690..3394
                     /locus_tag="PLL59_11720"
     CDS             2690..3394
                     /locus_tag="PLL59_11720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HMY24522.1"
                     /translation="MKRFYTNNIQGNIAFLENEEALHCSKVLRCRINDKVEILNGNGA
                     LLSGQISNIQKLDVQITIIGKIKEQIENANRISIGICLTKNPARIEWFVEKATEIGIV
                     SIFPLVSERTEKQNIKLERLQNIIIAAAKQSGQLHFPTLHTTQSLKSFLQETYQYEQK
                     FIAHCEGEKQQLKNVYQRNKNAIILIGPEGDFTKEEIAIAINKNYMPISLGNSILRVE
                     TAGVMACAIINSINND"
     gene            3387..4058
                     /locus_tag="PLL59_11725"
     CDS             3387..4058
                     /locus_tag="PLL59_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HMY24523.1"
                     /translation="MIKIFKHILLIFCICVLPIHAIAQVKTTTKLGLLKYKGGGDWYA
                     NPTSLPNLAKFCNQQLHLNLSADIDEVEPGSISIFNYPFVHITGHGNIIFTDQEAANL
                     RAYLIAGGFLHVSDNYGLDPFIRKAMKKVFPELDFVELPNNHPIYHQHFDFPNGLPKI
                     HEHDKKAAQGFGLIYEGKLVCFYDYECDLGDGWEDASVHKDTEEARQKALKMGANIVQ
                     YAFTK"
BASE COUNT         1409 a          754 c          690 g         1352 t
ORIGIN      
        1 atcgatgagc ttcttcgctt catccgcatt tgtaccttgc agacgcacaa taatcggaac
       61 cggaatttcc ccgatattgt tataggcatc aacaatgccc tgtgctacac ggtcacagcg
      121 aacaattcca ccgaaaatat ttacgagaat tgctttaaca ttcgggtctt tgaggataat
      181 acggaaggcc tgttcaacgc gggcggcatt tgcagtacca cctacatcca ggaagttagc
      241 aggatctcca ccggctagct tgataatatc cattgtggcc atggccaaac ccgcaccatt
      301 caccatacag cctacgttgc cgtccagctt tacataattc aggttgtgct caccggcttc
      361 cacttccgta ggatcttctt ccagaacatc ccgcatggca gcataatccg gatggcggaa
      421 aagtccgttt tcgtccagat taacctttgc atctactgca ataattttat catcagaagt
      481 ttttaaaact ggatttattt caaacatgct tgaatcagaa ccttcatatg ccttatacaa
      541 acgttgtgta aaatccacca tttctttgaa agcatttcct gacaatccta aattaaatgc
      601 aatttgacga gcttggaaag cttgcaagcc tattgttggg tcaatccatt ctttaaatat
      661 tttttctggc gtatgttctg caacctcttc gatgtccatt ccgccttctg tcgaatacat
      721 gattacattg cattcttttt ttctatctaa caacactgac atgtaaaatt ctttcgtttc
      781 tgatgcgcct ttataatagg catcttctgc gattaatatt ttatgaacca ctttgccctg
      841 tgcgccagtt tgtggcgtca ccaatgtcat acctaaaatt tgatctactt tttctttaag
      901 tgcttctaaa ttgggtgcaa acttaacgcc accacctttg ccacgaccac cagcatgtac
      961 ttgtgccttt acgaccacac cttcgctgcc tgtgcgtgcg ctaatcactt tccatgcgtc
     1021 tattgcttgt tcttttgttt cggcaactat gccttcttga actttaacgc catattgttt
     1081 taaaagttct ttaccttgat attcgtgtaa attcatcttt tgttttattt ttatgagcca
     1141 ataggaatat gttagaaaaa tggaatttca tttataatac tttgaaatac atttaacggc
     1201 acaaaagtag aaagtattct taaattttag aagtaaaagt aaaaagttta tcattaattt
     1261 tagtttttac ctataattgc ataaaattta ttattaaaac gccaatcgac atattaaaaa
     1321 aatcacagca tctagaattg ttggtgcatt tgcatcctta ttgtttcagt tatgctatta
     1381 ttgacaaaac acaacatcaa ctgattgatt ttgaagtaaa agtaataaat caacagcttg
     1441 gaaaattttt gcatttagac acgatagcgc tttggtttgc cgaccatgaa gaaacattta
     1501 gcctgccatt taaaacatct aaagtggcca tttactcgcc tgaatttacc attttgccag
     1561 aaaaaacaga taaaactgcc gagcttttca aattattagg ctatccgaat tctgacaata
     1621 ttacctattt gaaaaacaaa atttcagata ctttctatgt gtattattct ttgcctgata
     1681 agaccatcaa ttttatagaa aatcatcttc ccaatgttga atttttttgc attgactatg
     1741 gtttgttgaa tttttacaat agcaaattat cctttaaaaa ctatgttgct gccaatttat
     1801 atggtgagga attgacaatt tgctataaga gaaacaatga aaatttatat tacaataaat
     1861 ttcctgtaaa aggcaaagaa gatttattat actacttacg tttaacttac gaacatttgg
     1921 aactatcatt gaatgaattt ccaacttacc tttatggctt tattgaagaa aagtcgccga
     1981 tgtattcaac gacatacgga tacatacgga attttgaaat cgatagaaca ttaaaaaaca
     2041 cactttcact gcgaatgacg attgaagaca ttcctttgca ttattacatt aacttgattg
     2101 gattagattt atgagaatta tttcgggcac gctgaaaggg aaaagatttt atccgccaaa
     2161 aaatatacct gcaagaccaa ctactgattt tgccaaagag gctttattta atattttaaa
     2221 taatcatttt gatttttctg acataaaatt cctggattta tttgctggca ccggaagctt
     2281 ggatatggaa atgttctcca gaggttgtac agatattacc tctgttgaca tgagtaaaat
     2341 aagcttaggt tttatcaaaa aaatgagcga agaagtaaag attccgaatc ataaaataat
     2401 atttggtgac gccatacaat ttgcaaaaaa ttgcacacaa caatatgatt taattttcgc
     2461 tggtccgcct tatgcacttg agattatcga tgaaattcca gatattatct tcgacaaaaa
     2521 aatgttgaaa gaaaatggta tgtttatcct agagacttcg ccaaaacacg atttcacgga
     2581 acactcaaaa ttattagaag tgcgtaatta tggacaaact catttttggt tttttggtga
     2641 tgaaacgaat gatgatgatg atgaagaaga cgaataggaa taattcatta tgaaaagatt
     2701 ttacactaat aacatacaag gcaatattgc atttcttgaa aatgaagaag cattgcattg
     2761 cagtaaagta ttgcgttgca gaataaatga caaagtagaa atattgaatg gcaatggtgc
     2821 tttactcagc ggtcagattt caaacattca aaaactcgat gtacaaataa ctattattgg
     2881 aaaaatcaaa gagcagatag aaaatgcaaa tcgaatttct attggcattt gcttgacaaa
     2941 aaatccggca cgaatagaat ggtttgttga aaaagcaaca gaaataggca ttgtatcgat
     3001 atttccatta gtttcagagc gcactgaaaa acaaaatatt aagttagaac gattacaaaa
     3061 tataattatc gctgcagcta agcaaagtgg gcaattgcac tttcccacat tacatacaac
     3121 acaaagctta aaaagttttt tgcaggaaac atatcaatac gaacaaaaat ttattgcaca
     3181 ttgtgaaggt gaaaaacaac aattaaaaaa tgtatatcag cgtaataaaa atgcaataat
     3241 attgattgga cccgaaggcg actttacaaa agaagaaatt gcaattgcca ttaacaaaaa
     3301 ttatatgcct atttctttag gaaattctat attgcgtgta gaaacagccg gtgtaatggc
     3361 ttgtgccata ataaacagca tcaataatga ttaaaatttt taaacatatc cttcttattt
     3421 tctgtatttg tgtgctacct atacatgcta tagcacaagt aaaaacaaca acaaaattag
     3481 gattattgaa atacaaaggt ggtggtgact ggtatgcaaa tccaacttct ttacccaacc
     3541 ttgctaagtt ttgcaatcaa caattacact taaatctgag tgctgacatt gatgaagtcg
     3601 aaccaggaag tatttctatt tttaattatc catttgtaca tattactggt catggcaata
     3661 ttattttcac agatcaggaa gccgctaatt tgcgtgctta cttaattgct ggtggttttt
     3721 tacatgtatc agataattat ggcttagatc cttttatcag aaaggccatg aaaaaagtat
     3781 ttcctgaatt agattttgta gaacttccca ataatcatcc aatatatcat cagcatttcg
     3841 attttccgaa tggtttacca aagatacatg agcacgataa aaaagcagca caaggatttg
     3901 gccttattta cgaaggaaaa ttagtctgtt tttatgatta tgaatgcgat ttaggtgatg
     3961 gctgggaaga tgcttctgtg cacaaagaca cggaagaagc tcgacaaaaa gctttaaaaa
     4021 tgggcgcaaa catagtgcaa tatgccttta caaaataata ttgaattgca attaattcct
     4081 gtttgtttga aataaaaaaa tgaagattat cgcattataa tccagaagcg atattgctta
     4141 ttccgccgat accaccactg ccaagtccgc cgaaaattcc cgtatcaaaa aatccttttt
     4201 gtttt
//
LOCUS       DAOQQV010000160         4104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_10337_length_4104_cov_7.711287, whole genome shotgun sequence.
ACCESSION   DAOQQV010000160 DAOQQV010000000
VERSION     DAOQQV010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10337_length_4104_cov_7.711287"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..365
                     /gene="trxA"
                     /locus_tag="PLL59_11730"
     CDS             45..365
                     /gene="trxA"
                     /locus_tag="PLL59_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMY24524.1"
                     /translation="MAVEFTNDNFKEKVLDNNGVALVDFWAEWCGPCRLIGPVVEDLA
                     NEYDGKATIGKLNVDDNREIASEYGIMSIPTLLIFKNGQIVDKHVGVASKADLKKKIE
                     AALA"
     gene            470..1384
                     /locus_tag="PLL59_11735"
     CDS             470..1384
                     /locus_tag="PLL59_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMY24525.1"
                     /translation="MVLQQQDFFQFDQLDILARQVVEGFIIGLHKSPYHGFSVEFAEH
                     RLHNIGDSIKDIDWKVFARTEKLYTKKFEEETNLRCQIVIDISSSMLFPQQREKGKLN
                     KLEFSCVSAAALMHLLKKQRDAVGLTLFNQTIQVQTSAKTNALHHQLLMNELYRVLSK
                     NQLEQTTSAAKCLHQIADTIHKRSLVIIFSDMLDSMDSNELFLALQHLKHSKHEVVLF
                     HVHDKTKELDFNFENRPYKFVDMETGEELKLQPNQVKDIYVQKMKERTATIKLKCAQY
                     KIDFVEANINDGFQAILMSYLVKRNKMI"
     gene            1381..1857
                     /locus_tag="PLL59_11740"
     CDS             1381..1857
                     /locus_tag="PLL59_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24526.1"
                     /translation="MINKFDVHIEKDEDIKEHKNLYASDVLSKINAIPWNDEYKKCLA
                     SDDEYGTFFYVTFVDNSNLEYAFDVELYLFDDDEKNEINNQSPTLFTICYSYQEIIKE
                     KVFFGLFGEKEKIVDRVVYMDPVDFDFTLQCLQAFLRHDHAYLKKNIISSISFDEE"
     gene            1862..2629
                     /locus_tag="PLL59_11745"
     CDS             1862..2629
                     /locus_tag="PLL59_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMY24527.1"
                     /translation="MALSLNYKKIGDKGPAIIILHGLLGALDNWQTIAKQLSENHQVY
                     YIDQRNHGRSPHTDEIDYELLSQDVYDFCHQHNIEKASIIGHSMGGKVAMWYALQHTR
                     QVDKLFIVDIAPSFYDGGHESILFAMAEAPLKSTDKRETIDNFLQHRIQDFGTRQFIM
                     KNLTRNKNAHFEWKCNFEALISNYRKLMEFPKTTHQFLGQTYFIKGENSNYINQDNFD
                     TCDLYFPNNKIIEISNAGHWVHADNPTEFVAKIKPLL"
     gene            complement(2717..2971)
                     /locus_tag="PLL59_11750"
     CDS             complement(2717..2971)
                     /locus_tag="PLL59_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24528.1"
                     /translation="MKNLQQHCNSFKSIMNIAAVQSAKKVIIAVFCFSILLISTTSCS
                     RQSASGCGTWPTMKSNHYRSDVWPIKIKSNNQFAQYKGYN"
     gene            3134..4030
                     /locus_tag="PLL59_11755"
     CDS             3134..4030
                     /locus_tag="PLL59_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24529.1"
                     /translation="MNGIFCRFLFIFGIVLLINACIPTGTTPITRLPAYRGLYVNKFD
                     TIFKDTSKENTLLRWAKKHNFNALTLYGLGEIIPYSSKYNDLAAFISRAKNMYGILEV
                     NPTRGKAIDFTTHINAYNNSRSNNVEKFNWANLEREFWNGDNSFTFSYTQLQLMKNWG
                     LTQSPIIKVEEYIGWLRDSSGAPANAIREAQIADSIVLVVDRLLLHDYQQNLSFSYVQ
                     SRLDILGQKAIAQGKVLDVVIIFSAENPTFTGVYFTTHAFVDAYNFINNANNSATYTG
                     KAGIRLIGYQIYNYSHAAAARP"
BASE COUNT         1424 a          636 c          726 g         1318 t
ORIGIN      
        1 gtttattatt acaaaagatt attcacttaa atcaaataaa tatcatggca gttgaattca
       61 caaacgataa cttcaaagaa aaagtattag acaataatgg cgttgcatta gttgattttt
      121 gggcagaatg gtgtggccct tgccgtttaa tcggtccagt agttgaagat ttagcaaatg
      181 aatacgatgg aaaggcaacg attggcaaat taaacgttga tgacaatcgc gaaattgcat
      241 cagaatacgg cattatgagt attcctactt tgttgatttt taaaaatgga caaatcgttg
      301 acaaacatgt tggcgtagct tcaaaagcag atttgaagaa aaagatagaa gcagcattgg
      361 cataattcca atgtgagcgt aaataggaaa gttatacgta attatattta cgtataactt
      421 tttttaattt tattttgtcc aaatttacgc tatattgtta gcatatatta tggttctgca
      481 acaacaagac ttttttcagt ttgaccaact tgacatctta gcacgtcaag tcgtagaagg
      541 ctttatcata ggcttgcata aaagtccata tcatggtttt tctgtcgagt ttgccgaaca
      601 tagattgcat aatattggcg attctattaa agatattgac tggaaagttt ttgccagaac
      661 agaaaaatta tacactaaaa aatttgaaga agaaaccaat ctacgatgcc aaattgtgat
      721 agatatttct tcgtctatgt tatttcctca acaaagagaa aaaggcaaat taaacaaatt
      781 agaattttca tgtgtgtctg ctgctgcttt aatgcattta ttaaaaaaac aacgcgacgc
      841 cgttggactt acgcttttta atcaaaccat acaagttcaa acatcagcaa aaacaaatgc
      901 cttgcatcat caattattga tgaatgaatt gtatcgtgtg ctcagcaaaa atcaattaga
      961 acaaacaacg tctgcagcaa aatgcctaca tcaaattgct gatacgatac acaagcgttc
     1021 tttggtgatt atatttagcg acatgctcga tagcatggat tcgaatgagt tgtttttagc
     1081 tttgcaacat ttaaaacaca gcaagcacga agtggtgctg tttcatgtgc acgataaaac
     1141 taaagaatta gattttaatt ttgaaaatag gccatataaa tttgttgata tggaaacagg
     1201 cgaggagctg aaactccagc ctaatcaggt aaaagatatt tatgttcaga aaatgaaaga
     1261 acgcacggca acaattaaat tgaaatgtgc acaatacaaa atagatttcg ttgaagccaa
     1321 tatcaacgat ggttttcaag cgatattaat gtcatattta gtgaaaagaa acaagatgat
     1381 atgatcaata aattcgatgt acatatagaa aaagacgaag atattaaaga gcataagaat
     1441 ctttatgctt cagatgtact gtcgaaaatc aatgccattc cttggaatga cgaatacaaa
     1501 aaatgcctag catctgatga tgaatacggc actttttttt atgtgacctt tgtcgataat
     1561 tctaatttag aatatgcttt cgatgttgaa ttatatcttt ttgatgatga cgaaaaaaat
     1621 gaaataaaca atcaaagtcc gactttattt accatctgtt attcttatca agaaataata
     1681 aaagaaaaag ttttctttgg actgtttggc gagaaagaga aaattgttga ccgtgttgtc
     1741 tatatggatc cagtagattt tgattttaca ttgcaatgtt tacaggcatt tttgcgacat
     1801 gatcatgcgt atctcaaaaa gaatattatt tctagcataa gcttcgacga agaataataa
     1861 aatggcatta tcattaaact ataaaaaaat aggcgataaa ggtcctgcaa ttatcatttt
     1921 acatggcctt ttaggtgcat tagataattg gcaaacgatt gcaaaacagt tgtctgaaaa
     1981 ccatcaagtt tattatattg accagcgcaa tcatggacga tcgccacata cggatgagat
     2041 agattacgaa ttgctatcac aagatgtata tgatttttgc catcagcata atattgaaaa
     2101 ggctagcatt atcggacatt ctatgggtgg caaagtagcc atgtggtatg cattgcaaca
     2161 tacacgacag gttgacaaat tatttattgt ggacattgca cctagttttt atgatggtgg
     2221 gcatgaatcc attttatttg ctatggcaga agcaccatta aaatcaactg ataagcgcga
     2281 aaccatagac aactttctgc aacatagaat tcaagatttc ggcacacgac agtttatcat
     2341 gaaaaattta acgcgcaata aaaacgctca ctttgaatgg aaatgtaatt tcgaagcatt
     2401 aatcagtaat tacagaaaat taatggaatt tcctaaaaca actcatcagt ttttaggtca
     2461 aacctatttt atcaaaggcg aaaattctaa ttacatcaat caagataatt ttgatacttg
     2521 cgacctgtat tttccaaaca acaaaatcat agaaatttca aatgcaggac attgggtgca
     2581 tgctgataat ccaacagaat ttgttgcaaa aattaaacca ttattataac aatgaaaata
     2641 ctgcttccga tggaagcagt ccaaaaacac ttcaaaatta caatatgatt gaatgtttta
     2701 tctttataag ctaagcttaa ttatatcctt tatattgtgc gaattgatta ttgcttttga
     2761 ttttaatcgg ccaaacatcg cttctgtaat gattgctttt catagtaggc caagtgccac
     2821 aaccactagc tgattggcga ctgcatgatg ttgtactgat taacaatatg ctgaagcaaa
     2881 atacagcgat gattactttt ttagcggatt gtactgctgc aatgttcatg atacttttga
     2941 acgagttgca atgttgttgt aagtttttca taattttggt tctaaagtgt ttataatgtt
     3001 atggtgtttt gttgatgtaa atttgcaaca cttttgtttt gctgtgttgt ataaatgagt
     3061 aaaaggaaaa tagaatgtgt aaatggtcaa ctattgcatt ttatacgtac atttattaaa
     3121 tttcagctac attatgaacg gtattttttg cagattttta ttcatttttg gaattgtatt
     3181 gctgataaat gcatgtattc cgacaggtac tacaccaatc acaaggcttc cagcgtatag
     3241 gggtttgtat gtcaataaat tcgatacgat ttttaaagac acatcaaaag aaaatacgct
     3301 attgcgctgg gcaaaaaagc acaatttcaa cgcacttact ttgtatggct taggcgaaat
     3361 cattccttat tcttctaaat acaatgactt agctgctttt atttcaagag caaaaaatat
     3421 gtatggcatt ttagaagtaa atccaacaag aggcaaggct attgatttta caacacatat
     3481 aaacgcatat aataattcgc gtagcaataa cgttgaaaaa tttaattggg caaacttaga
     3541 acgcgaattt tggaatggcg ataattcctt tactttttca tacacacaat tacaattaat
     3601 gaaaaattgg ggtttgacac aatcgccgat tattaaagta gaagagtata tcggctggct
     3661 tcgcgattcg tctggtgcac ctgcaaatgc tatacgtgaa gcgcaaattg ctgatagtat
     3721 tgtgctagtg gtagatagac tattgctgca tgattatcag caaaatttat cattttctta
     3781 tgtccaatca agattagata ttttaggcca aaaagctata gcgcaaggca aagttttaga
     3841 tgttgtgatt atatttagtg cagaaaatcc taccttcacc ggtgtttatt ttacaacaca
     3901 tgcttttgtc gatgcttata attttattaa taatgcaaat aattcagcga cttacacagg
     3961 caaagcaggt attcgtttaa ttggatatca gatatataat tattcgcatg cggcagcagc
     4021 tcgcccttga tggattaatg taaaggacgt ttttttacca tgccgcctct aatgtctttt
     4081 actaacgaag aaacgataaa agca
//
LOCUS       DAOQQV010000161         4036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_10626_length_4036_cov_6.957297, whole genome shotgun sequence.
ACCESSION   DAOQQV010000161 DAOQQV010000000
VERSION     DAOQQV010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10626_length_4036_cov_6.957297"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..1421
                     /gene="atpB"
                     /locus_tag="PLL59_11760"
     CDS             342..1421
                     /gene="atpB"
                     /locus_tag="PLL59_11760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HMY24530.1"
                     /translation="MPFFAIANEAPSHGAESHENADATAIVVEHVMNHIADANEFHIA
                     TIDGHHYSLHLPCILLRDGNLSIFNSAKFHHGEHEVEGYKLDPHTGRVVDAATGQRSA
                     NIANYFSAADSSKFLDLSITKNVFTYLLSFILLTVILLIVKNAYVKNKNASPKGIQSV
                     IEPVVSFIMNDVAKENIGPKYEKFVPFLLSLFFFILCNNLIGLIPFFPGSANTTGNIA
                     FTCVLAVIAFVVININGNKSYWGHIFAMPGVPKWVLVILTPVEIVGMFIKPMALMLRL
                     FGNILGGHIAILSIVSLIFIFGQMGKSITGASVGSALAIPIVIFVNAMELFVAFLQAY
                     VFTLLTAIFIGAAVEEHEHHGAEHH"
     gene            1458..1667
                     /gene="atpE"
                     /locus_tag="PLL59_11765"
     CDS             1458..1667
                     /gene="atpE"
                     /locus_tag="PLL59_11765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HMY24531.1"
                     /translation="MTITGIAALGAGLAALGAGIGIGLIGSNATQAIARQPEASGDIR
                     GAMILTAALVEGAALIAIIFSFLMK"
     gene            1789..2301
                     /gene="atpF"
                     /locus_tag="PLL59_11770"
     CDS             1789..2301
                     /gene="atpF"
                     /locus_tag="PLL59_11770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HMY24532.1"
                     /translation="MHMLAFDAMSLILPDLGLLFWTTVIFLLFWFLMGKFAFTPIVTA
                     IKDREKSIDDALSAADKAKSEMMQLQSDNAALLKEAKEESAKILKEAKDVKESVIADA
                     HNKAKEEAAKIIAAAKVEIEAQKKAALAEVKNEVSKLSLDIAQKVLGRELETAKDQQS
                     YINTLVDKLN"
     gene            2301..2843
                     /gene="atpH"
                     /locus_tag="PLL59_11775"
     CDS             2301..2843
                     /gene="atpH"
                     /locus_tag="PLL59_11775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HMY24533.1"
                     /translation="MSAEKVGKRYAKALFDESAKAGTLDAVYADLNLLDTTLKASKEL
                     KALFKSPVVQPAKKLSIIQQIFSGKIKTESDSFLKLLLQHGREAYIQDIIGAFFRQYN
                     ESKSISEVTVTTATELDAANEQNIIKYIKATSGYSNIKITKKVDAALIGGFIVDFGGK
                     MYDNSVRYKLNKIKKEISLN"
     gene            2860..>4036
                     /gene="atpA"
                     /locus_tag="PLL59_11780"
     CDS             2860..>4036
                     /gene="atpA"
                     /locus_tag="PLL59_11780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HMY24534.1"
                     /translation="MVEVRPDEISAILRQQLGNVSTEAQLEEVGTVLQVGDGIARVYG
                     LSQVKAGELVEFANGMNAIALNLEEDNVGVVLMGPSDEIKEGDSVKRTGLIASLKVGE
                     GMLGRVVNTLGQPIDGKGPIGGELFEMPLERKAPGVIYRQPVTEPLQTGIKSIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQKEFYDKGEPVFCIYVACGQKASTVANIAQT
                     LEENGAMPYTVIVAANASDPAPIQFYAPFAGAAIGEFFRDTGRPALIVYDDLSKQAVA
                     YREVSLLLKRPPGREAYPGDVFYLHSRLLERAAKVINNDSIAATMNDLPESLKGKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEANLFNAGVRPAINVGISVSRVG
                     "
BASE COUNT         1352 a          671 c          815 g         1198 t
ORIGIN      
        1 ctatggttag tggaaagatt ttgaaaaaag cagcaaccac tttatccggt gctaaagcag
       61 attctatttt tattgaaatg gaattttctg acgatccagg taattggtat atttattccg
      121 gtcatagaga tacaggtcaa cctgaagatc agtattaata gtttcttata tagaactttc
      181 aataaaaaag ccaccttagt gtggcttttt tatttgtctt aacacacatt taatttccca
      241 acaaagccaa cattcctcta taaatttgta tatttgcagc gcaattttag agtaaagtgg
      301 taagaaaaca acatatattc cttgtattgt tattggcatt aatgccattt tttgcaatag
      361 caaacgaagc accatcacat ggcgctgaat cgcacgaaaa cgcagacgca acggcaattg
      421 ttgtagaaca tgtgatgaat cacattgctg acgcgaatga gtttcatatt gctaccatcg
      481 atggacatca ttattcatta catttaccat gtatcctatt gcgcgatggt aatttgtcta
      541 ttttcaactc agctaagttt caccacggcg aacatgaagt tgaaggctat aaactagatc
      601 ctcatactgg cagagtagtt gatgcagcaa caggccaacg cagtgcaaac attgcgaact
      661 acttctcggc tgcagattct tctaagtttt tagatttatc gattactaaa aatgtattca
      721 catacctatt aagctttatt ttacttacag taatattatt gatagtaaaa aatgcttatg
      781 tgaaaaacaa aaacgcatca ccaaaaggca tacaatctgt cattgagcca gtggttagtt
      841 ttataatgaa tgatgttgca aaagaaaata taggacctaa atatgagaaa ttcgtgcctt
      901 tcttattgtc attattcttc tttattttat gcaataattt aataggctta attccatttt
      961 tcccaggtag tgccaacacg acaggcaata ttgcattcac atgtgtattg gcagttattg
     1021 catttgtagt aattaatatt aacggcaata aaagttactg gggtcacata tttgctatgc
     1081 caggcgtacc aaaatgggta ttggttattc ttacgccagt agaaatcgtg gggatgttta
     1141 ttaagccaat ggcattaatg ctgcgtttgt ttggtaatat cttaggtggc catattgcta
     1201 ttttaagtat tgtgagtttg atatttattt ttggtcaaat gggaaaaagc attacaggtg
     1261 cttccgtagg aagtgcattg gctataccaa ttgtaatatt tgtaaatgct atggaattgt
     1321 ttgtagcatt cttacaagca tacgtgttta ctttgttgac ggcgattttt attggcgcag
     1381 cagtagaaga acacgaacat catggagcag aacatcatta atttaattta aataaaaaca
     1441 attaaaaaac attagttatg acaattacag gaatcgcggc acttggtgca ggtttagcag
     1501 ctttaggcgc aggaatcgga atcggtttaa tcggaagcaa cgctactcaa gcaattgctc
     1561 gtcaaccaga agcatctggt gatatcagag gtgcgatgat cttaacagca gcattagtag
     1621 aaggtgcagc attaatcgct atcatcttct cattcttgat gaaataatat agggaagctc
     1681 aatgaggata tctcattgag cttttaattt tttgaatttc atcttttgaa aattaatttc
     1741 gctaaatagc gagtaactta ccttaagagg taatcaaaac ggaatcgtat gcatatgtta
     1801 gcatttgatg caatgagttt aattctaccc gatttgggtt tgctgttctg gacaacagtt
     1861 atctttttat tgttctggtt tttgatgggt aaatttgctt ttacacctat cgttaccgcc
     1921 attaaagata gagaaaaatc aatcgatgat gcgctttctg cagcggataa agcaaagtct
     1981 gaaatgatgc aattgcaatc agataatgca gcattgctaa aagaagcaaa agaagaaagt
     2041 gctaaaattt tgaaagaagc taaagatgtg aaagaaagcg taattgcaga tgcacataat
     2101 aaagcaaaag aagaagctgc aaaaataatc gcagccgcaa aagtagagat tgaagcacaa
     2161 aagaaagcag ctttagcaga agtgaaaaat gaagtaagca aactctcttt agatattgca
     2221 caaaaagtat taggcagaga attagaaact gctaaagatc aacaatctta tatcaatacg
     2281 ctagtagata aattgaacta atgtcagcag aaaaagtagg aaaacgatat gcaaaagcat
     2341 tatttgatga gtcggcaaaa gctggcactt tagatgctgt gtatgcagat ttaaatttgt
     2401 tggatactac attgaaagcc agcaaagagt taaaagcttt atttaaaagc ccagtcgtgc
     2461 aaccagctaa aaaattgtcg attatccaac aaatttttag tggcaaaatc aaaacggaat
     2521 ctgattcgtt tttgaaactt ttattacaac acggaagaga ggcttatatc caagatatta
     2581 ttggcgcatt ctttcgccaa tataacgaaa gcaaaagtat ctctgaggta acagtaacaa
     2641 cagctacgga gctagatgca gcaaatgagc aaaatatcat caaatatatc aaagcaacgt
     2701 ctggctatag taatataaaa attactaaaa aagtagatgc tgctttaatc ggcggtttta
     2761 ttgtagattt tggtggcaaa atgtacgata actcagtccg atataaattg aataaaataa
     2821 aaaaagaaat ttctttaaac taagataaac aataatacaa tggtagaagt aagaccagat
     2881 gaaatatcag caatactacg tcagcaatta ggcaacgttt ctacagaagc tcaattggaa
     2941 gaagtaggca ccgtattaca agtaggtgac ggtatcgcac gtgtgtacgg cttgtcacaa
     3001 gtgaaagcgg gtgagttagt agaatttgct aatggcatga atgcgatagc attgaactta
     3061 gaagaagaca atgtaggtgt cgttttaatg ggtccttcag acgaaattaa agaaggtgac
     3121 agcgtgaaac gcactggttt aattgcctct ttaaaagtag gcgaaggcat gttgggtcgt
     3181 gtggtaaata cacttggaca acctattgac ggaaaaggtc ctatcggtgg cgaactattc
     3241 gagatgccat tagaaagaaa agcacctggt gtaatttatc gtcagccagt aacggagcct
     3301 ttgcaaacag gtatcaaatc tatcgatgcg atgattccaa ttggtcgtgg tcaacgtgag
     3361 ttgattatcg gtgaccgtca aactggtaaa acagcaatcg ctatcgatac tatcatcaat
     3421 caaaaagaat tttacgataa aggagaacct gttttctgta tctatgtggc ttgtggtcaa
     3481 aaagcatcta cggttgcaaa catcgcacaa acattagaag aaaacggtgc aatgccatat
     3541 acagtaatcg ttgcggctaa cgcatcagat ccagctccga ttcaattcta tgcacctttt
     3601 gctggtgctg ctattggcga gttcttccgc gatacgggtc gtcctgcatt aatcgtttac
     3661 gatgatttat ctaaacaagc agttgcttac cgcgaggttt ctttattgtt aaaaagacct
     3721 ccaggtcgtg aggcatatcc tggtgacgta ttctacttac actctcgttt gttagaacgt
     3781 gcggcaaaag tgattaacaa cgacagcatt gctgcaacga tgaacgattt accagaatca
     3841 ttaaaaggaa aagtaaaagg tggtggttct ttaacagcat taccaattat tgaaacacaa
     3901 gcaggcgacg tttctgcata tattccaacc aacgtaattt caattactga tggtcagata
     3961 ttcttggaag cgaacttgtt taatgcaggt gttcgtccag caatcaacgt aggtatttca
     4021 gtatctcgtg tgggtg
//
LOCUS       DAOQQV010000162         3964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_10958_length_3964_cov_8.872602, whole genome shotgun sequence.
ACCESSION   DAOQQV010000162 DAOQQV010000000
VERSION     DAOQQV010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10958_length_3964_cov_8.872602"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2534)
                     /locus_tag="PLL59_11785"
     CDS             complement(<1..2534)
                     /locus_tag="PLL59_11785"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMY24535.1"
                     /translation="MQKIIILTTLLLFSITISAQTRLVVKGKISDENNNAISNVSVIQ
                     VGTTNGTTTSHKGYYAIEVEYDDSCVIEFSGLAYLRKSIVLFPSNKATIYKDITLSKG
                     DYAIDTVEITATKTEVGKSVIEAKSAEYFPNPTGDAGTLVKTMGQGVQSNNELSGQYN
                     VRGGNYDENLVYINDFEIYRPFLVSSSQQEGLSFTNLDLTDKLSFSGGGFESKYGDKM
                     SSVLNIKYKNPTTFKGSVMASILGVNAHVEGAAYPKKDTANENAKFTYLVGLRYKSNG
                     YLLGSLDKKGEYNPNFFDVQADFHIKPNVKHDIEILVNHSFNDFKFTPVTEETRAGTF
                     DQLIRFLVDFDGGEKDRFQNTMLGISYKYLPNTKLSLKFLTTFWKMRESETFNITGYY
                     SLDEIEIDPSNENFGNVKDNLGIGTFQDWARNKLDAVVSNFSHIGKYIIKDKKNEKEK
                     HLLEWGSTFQFEKINDELSEWQRIDSAGYSIPYSGTSIQFPYRLKTANNLTSFRVHGY
                     FQDTWNIINTDSTKLILTAGVRYNFWNVNKEFLVSPRVQLLYHPKLKSDVTFRLAGGM
                     YVQPPFYRELRGFDGNINKNIKAQQSYHVVIGADYNFKIKKRNFKLTAEAYYKHIRNL
                     NPYEIQDVRIRYFANNDAKGYATGIDVRLFGEMIKGADSWITLSYLNTRENLNNDMYY
                     TYRDSLGKIYNSPNFTNNRITDTLTNYPGYIRRPTDQALFVSLYFQDYIEKYKRFKVH
                     FNLVIGTGLPFGPPDKNRYKDVLKMPPYRRLDIGFSALLLNGAKRAVKKPNSFGSKFE
                     NIWASFEVFNIIGIRNTLSYRWIKDTENNLWAIPNYLTSRRFNVKL"
     gene            complement(2555..3211)
                     /gene="rpe"
                     /locus_tag="PLL59_11790"
     CDS             complement(2555..3211)
                     /gene="rpe"
                     /locus_tag="PLL59_11790"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HMY24536.1"
                     /translation="MNKPIIAPSILSANFANLSTDFEMLHRSAAEYIHVDVMDGVFVP
                     NISLGLPVIQAMRKLTTKTFDVHLMIVEPDKYIDNFKQAGADILTVHVEACTHLHRTV
                     QNIHSVDMRAGVAINPHTPIHLLEDIINDIDLVCIMSVNPGFGGQKFIENTYTKVAAL
                     KELIQKKNATALIEIDGGVSLQNAKKLVEVGANVLVAGNTVFNSENPEQTIQLLKNIV
                     "
     gene            complement(3295..3483)
                     /locus_tag="PLL59_11795"
     CDS             complement(3295..3483)
                     /locus_tag="PLL59_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24537.1"
                     /translation="MRQNRWLSYFYIFCRIVLALGFIPSGITKIIGERFASGLSIQHP
                     MGQYLEALHHTSYYYTII"
     gene            complement(3575..>3964)
                     /locus_tag="PLL59_11800"
     CDS             complement(3575..>3964)
                     /locus_tag="PLL59_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMY24538.1"
                     /translation="ELLEMKPDELAVLRATLTFHLSVDTPVGDDDDTTSLDLMTDDSI
                     STPDRQLIDESLHDELEHALQSLSDREIEIVKYYYGLNEFNQSYSLDEIGIKMNLTRE
                     RVRQLKDKAIRKMRRFAITKELKSYLN"
BASE COUNT         1248 a          690 c          628 g         1398 t
ORIGIN      
        1 tgtaatttta cattgaatct tcgcgaggtg agataattag gaattgccca taagttattc
       61 tccgtatctt ttatccaacg gtatgaaagc gtatttcgaa ttccaataat attaaacact
      121 tcaaagcttg cccaaatatt ttcaaacttg cttccaaagc tattaggctt tttcacagca
      181 cgttttgcac catttaatag taatgccgaa aatccgatat ctaaacggcg ataaggtggc
      241 attttcaata catctttata tcgattttta tctggtggac caaatggcaa acctgtgcca
      301 atgactaaat taaaatgcac tttaaagcgt ttgtattttt caatataatc ttgaaagtat
      361 aaactcacaa acaaagcttg atctgttggt ctgcgaatat aacctggata atttgttaag
      421 gtgtctgtga ttctgttatt tgtaaaattt gggctattat aaatttttcc taagctatca
      481 cgataggtat aatacatatc gttgttcagg ttttctcgtg tgtttaaata cgataatgtg
      541 atccaagaat ctgcgccttt tatcatttca ccaaacaagc gtacatcaat gcctgttgca
      601 tatcctttag cgtcattatt tgcaaaatat ctaatgcgta catcttgtat ttcgtatgga
      661 tttaggttgc gaatatgttt ataatatgct tcagccgtta gtttaaaatt tctttttttg
      721 attttaaaat tatagtctgc accaattaca acatgatagg attgctgcgc cttaatgttt
      781 ttattaatgt ttccatcaaa gccacgcaac tctctgtaaa atggaggttg tacatacatg
      841 ccaccagcca atctaaaggt aacgtcactt tttaattttg gatgatacaa gagttgaact
      901 cgtgggctca ctaaaaattc tttattgaca ttccaaaaat tataacgcac accagcagtg
      961 agtattaatt ttgttgaatc ggtgtttatg atattccacg tgtcttgaaa ataaccatgc
     1021 acacgaaagg aagtaagatt atttgctgtt ttcagtcgat acggaaattg aatagatgtg
     1081 ccagaatagg gaattgaata tcccgctgaa tcgatgcgct gccattcgct tagctcatca
     1141 ttaattttct caaactgaaa agtcgaaccc cattctaata gatgtttttc tttctcgttt
     1201 tttttgtctt taataatata ttttccaatg tgagaaaaat tgcttacaac agcgtctaat
     1261 ttattacgag cccaatcttg aaatgtacct atgcctaaat tatcttttac atttccaaaa
     1321 ttttcatttg atgggtcaat ttctatttca tctaaagaat aatagccagt gatattaaat
     1381 gtttccgatt cacgcatctt ccaaaacgta gttaaaaatt ttaaactcag ttttgtgtta
     1441 ggcaaatatt tataagaaat tcccaacata gtattttgaa atctatcttt ttcgccgcca
     1501 tcaaaatcta ctaaaaatcg aatgagttgg tcaaatgtgc ctgctcgtgt ttcttctgta
     1561 acaggcgtaa atttaaaatc gttgaaagag tgattgacaa gaatttcaat atcgtgtttt
     1621 acatttggtt ttatatgaaa gtcggcttgc acgtcaaaaa agtttggatt gtattcgcct
     1681 ttcttatcta agcttcctaa taaatagcca ttagatttat aacgcagacc tacgagatag
     1741 gtgaattttg cattctcatt agcggtatct tttttcggat atgctgcacc ttcaacatgt
     1801 gcatttacac ctagtattga tgccatgaca gagcctttaa atgtcgtagg atttttgtat
     1861 tttatgttta atacagatga cattttatcg ccatatttag actcgaaacc gccaccagaa
     1921 aagctgagtt tgtctgttaa atctaaattg gtaaagctca atccttcttg ttgactacta
     1981 gacactaaaa atggtctgta gatttcaaaa tcattgatat acaccaaatt ttcatcgtaa
     2041 tttccaccac gaacattata ttgaccagat aattcattgt ttgattgtac accttgaccc
     2101 atcgtcttta ctaaagtgcc agcatcgcca gtcggatttg gaaaatattc agcactttta
     2161 gcttcaataa cagactttcc aacttcggtt tttgtagcag taatttcaac tgtgtcgatg
     2221 gcataatcgc ctttagataa tgtgatatct ttatatatag tagctttatt ggaaggaaat
     2281 aagacaatgg attttctgag gtatgctaat ccagaaaact ctatcacaca agagtcatca
     2341 tattctactt caatagcata atagccttta tgacttgttg tcgtgccatt ggtggttcca
     2401 acttgaatca cagagacatt tgaaatagca ttattatttt cgtcacttat ttttcctttt
     2461 acgactaatc ttgtttgtgc cgaaatagta atactaaaaa gcaggagagt agttaatata
     2521 atgatttttt gcatcatagt ataaacgatg aaaattacac tatatttttt aatagttgaa
     2581 tagtctgttc aggattttct gaattaaaaa cagtattgcc ggcaactaat acattggcac
     2641 caacttctac aagttttttt gcattttgta aagaaacacc accatcaatt tcaattaaag
     2701 cagtagcatt ttttttctga attaattctt tcagcgcagc aactttcgtg tatgtatttt
     2761 ctataaattt ttgaccacca aagccaggat ttacactcat gatacatact aaatcgatat
     2821 cattgataat atcttctaaa agatgaattg gtgtgtgcgg attgatggct acaccagcgc
     2881 gcatatcaac gctatgtata ttttgtacag tccgatgtaa gtgcgtgcaa gcttctacat
     2941 gcaccgttaa aatatcggcg cctgcttgtt tgaaattgtc aatatattta tctggttcga
     3001 caatcatcaa gtgcacgtca aaagtttttg ttgttagctt gcgcatcgct tgtattactg
     3061 gcaatcctag cgaaatattt ggcacaaaaa cgccatccat tacatcaaca tgtatatatt
     3121 ccgctgcgct acgatgcaac atttcaaaat cagtacttaa gtttgcaaaa tttgcagata
     3181 aaatcgatgg tgctatgata ggtttattca ttgcagtaaa gataaaagtt atttgttaat
     3241 agttattagt aagcttacaa tttaaatcaa taaatagaca tagtttttgt gttatcaaat
     3301 aatagtatag taatatgaag tatgatgcaa ggcctctaaa tattgaccca ttggatgttg
     3361 aatggataaa ccacttgcaa atcgttcacc aataattttt gtaattcctg aaggtataaa
     3421 tccaagtgcc aaaacaattc gacaaaaaat atagaagtaa gaaagccatc tattttgtct
     3481 catatgctca taaaattggt ctagtcttaa gaggatagac attacttcta ttaaaatatt
     3541 taatggctaa ttaatcaata aaaaaaatga tactttaatt caaataagat ttcaattctt
     3601 tagtgatagc aaatcttctc attttacgaa ttgctttatc ttttaactga cgcacgcgct
     3661 cacgtgtgag attcattttt attccaatct catctaagct gtacgattgg ttaaactcat
     3721 tcaaaccgta gtaatatttt actatttcaa tttctctgtc tgataaagat tgcaaagcat
     3781 gttctaattc atcgtgtaat gattcatcaa tcaattgtct gtctggtgtg gaaattgaat
     3841 cgtccgtcat taaatccaaa cttgtcgtat catcatcatc acctacaggt gtgtccacag
     3901 ataaatgaaa ggtaagtgta gcacgtaata ccgctaattc gtcaggcttc atttccaata
     3961 attc
//
LOCUS       DAOQQV010000163         3963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_10965_length_3963_cov_7.398925, whole genome shotgun sequence.
ACCESSION   DAOQQV010000163 DAOQQV010000000
VERSION     DAOQQV010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10965_length_3963_cov_7.398925"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..919)
                     /locus_tag="PLL59_11805"
     CDS             complement(215..919)
                     /locus_tag="PLL59_11805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY24539.1"
                     /translation="MKIKNAKILITGGSSGIGLATAKMLIKKGAQVVISGRNKKTLKK
                     TAEEIGAIAIACDVTNENDVIKLVKNTIAQLNGLDVLINNAGFGYFDALQNIDTEKFK
                     AIIETNVTGAMLCGRECAKYFIKQQYGNIINVASTSGLKGHQNGTAYSTSKWALRGMT
                     ECWREELRPYNIRVMMVHPSEVQTNFVINSGRPKRDFNNTKLEAQDVAHLIVSMLELR
                     DVGFIPEATIWSTNPK"
     gene            complement(968..2740)
                     /locus_tag="PLL59_11810"
     CDS             complement(968..2740)
                     /locus_tag="PLL59_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMY24540.1"
                     /translation="MKILLYYLQRYKWLVLLTFILAIINQSFSLLDPYIFGKIFDDYA
                     THPHTKIVDGKQFSRTSSEFIKGVLFLIACAISVAMISRIAKAFQDYCMNVVIQRFGA
                     DVYTDGLKHALQLPFQAFEDQRSGETLNILQKVRQDSERFMQNFINVLFVAVIGIVFV
                     IIYSAFVYPGLILMYFIASFLLGTSMNILSKKIKTVQATITQKTNILAGATTESLRNI
                     ELIKSLGLTQQEVNRLNDTTLKILNLELDKVKKIRSISFVQGTLVNSLRQVIMFIILM
                     LIFKDHLTAGQLITLQFYSFFIFNPLQEMGNVIMSYREAEASLKNFEKLMNTAVERKP
                     QVALSLHAIESLSFDNVGFQHQSATTNAIEHISFTAKKGDTIAFVGPSGAGKTTLVKL
                     LVGLYQAQSGKILYNNLDAENIDIEELRAQIGFVTQDTQLFAGTIKENLLFVNPSASD
                     DEIYDVLNKSACQSLLARSDNGINTVIGEGGMKISGGEKQRLSIARALLRQPKIMVFD
                     EATSALDSITENEITQTIKNLISKKEHITVMIAHRLSTIMHADTIYVLEKGKIIESGN
                     HQHLLAEKGLYYAMWRQQIGERER"
     gene            complement(2850..3395)
                     /locus_tag="PLL59_11815"
     CDS             complement(2850..3395)
                     /locus_tag="PLL59_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24541.1"
                     /translation="MKKLLTTVMLAFAAIAVFAGDQIFLHSGKTIDGKVVRVDDFKVI
                     FKYEGEDAEQTMSKKAIEKIIYSSGREEPISEKIVINGKEDWEKVEILLDVSEIVGLK
                     KVDEIKGKSTGGFSGSKSDKKANKKLLEAAAEMKCPFVFMTSESKSSGGGWGGWGGGI
                     ASIASANQSLKKGIAYTYNDK"
     gene            3659..>3963
                     /locus_tag="PLL59_11820"
     CDS             3659..>3963
                     /locus_tag="PLL59_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMY24542.1"
                     /translation="MLYALIGLIVLGIFLSFVTVQQGTIAVITMFGKYQRILGPGLNM
                     KIPFFEQVYKRVSIQNRSVELEFQAITIDQANVYFKAMLLYAVVNTDEATIKNVAFK"
BASE COUNT         1180 a          704 c          667 g         1412 t
ORIGIN      
        1 gagtataaga attttttgat gataggaaag atgttctcgg tttgtacatt aatttttcca
       61 gtttccattt tttatagatg attagatttt aagaaatgag atttgagaaa cactattcaa
      121 aagttgttcc aatggaaaat agctgacaag ttggcatttt attttttacc acataagaaa
      181 cttaagtagc taaagttcct aatgtgcttg atttttattt tggattcgta ctccaaatag
      241 tagcttcagg aatgaaacca acatcgcgca gctctaacat agaaactata agatgtgcaa
      301 catcttgcgc ttctaatttg gtgttgttaa agtcgcgttt tggcctgcca gaatttatca
      361 caaagttcgt ttgcacttcg cttggatgca ccatcatcac ccgaatatta taaggcctga
      421 gttcttcgcg ccaacattcc gtcatgccac gcaaagccca ttttgaagta gaatatgccg
      481 taccgttttg atgtcctttt aatccagaag ttgaggccac attgatgatg tttccatatt
      541 gttgtttgat gaaatattta gcacattctc tgccacacaa catggcgcca gttacatttg
      601 tttctatgat ggctttaaat ttttcggtat ctatattttg caacgcatca aaatatccaa
      661 agcctgcatt attgattaaa acatctaatc cattgagttg cgcaatcgtg tttttcacca
      721 atttaatgac atcattttca tttgttacat cacaagcaat tgcaatagct ccaatttctt
      781 cggctgtttt ttttagtgtt tttttattgc gtccactaat taccacttgt gcgccttttt
      841 taatcagcat ttttgccgtt gctaatccaa tacctgagct gccgcctgta attaatattt
      901 tggcattttt aattttcatg ttaatgattt ttttgaagat aagaaatctg agtgcaaagt
      961 aaaaagatta acgctcccgc tcaccaatct gttggcgcca catagcataa tacaatcctt
     1021 tctctgctaa taaatgttgg tgattgccgc tttcaataat ttttcctttt tctaatacat
     1081 aaattgtgtc tgcatgcata atcgtagata agcgatgtgc aatcataact gtgatgtgtt
     1141 cttttttcga aattagattt ttgatggttt gcgtaatttc attttctgta atcgagtcga
     1201 gtgccgatgt ggcctcatca aacaccatta ttttgggctg tcgcagtagt gcacgggcaa
     1261 tagataaacg ttgtttttct ccaccagaaa tcttcatgcc accttcgcca attacagtgt
     1321 tgatgccatt gtcgctacga gcgagcaagc tttgacaagc agacttattt aatacatcat
     1381 agatttcatc atcgcttgca cttggattga caaacagtaa attttctttt attgtgccgg
     1441 caaataattg tgtgtcttgt gttacaaatc caatttgtgc gcgtaattct tcgatgtcta
     1501 tattttccgc atcaagatta ttgtataata tttttcccga ttgtgcttga taaagtccta
     1561 ctaataattt taccaaagta gttttaccag caccagacgg accaacaaat gcgatagtgt
     1621 cgcctttttt agctgtaaac gaaatatgtt caatggcatt tgttgtagct gattgatgtt
     1681 gaaatccaac attgtcaaac gataaagatt caatggcatg taggctcaat gcgacttgtg
     1741 gcttgcgttc gacggccgtg ttcattagct tttcaaagtt ttttagtgat gcttccgctt
     1801 cgcgataact catgatgaca ttgcccattt cttgcaatgg attgaaaata aaaaacgaat
     1861 aaaattgaag tgtgattaat tgaccagcag ttaaatggtc tttaaaaatc aacatcaaaa
     1921 taataaacat aataacctgc cgcaacgaat tgaccaaagt gccttgcaca aaagagatac
     1981 tgcgtatttt ttttacttta tctaattcta aatttagaat tttaagtgtg gtgtcattga
     2041 gacgatttac ttcttgctgt gttaaaccaa ggctttttat taattcgatg ttgcgcaaac
     2101 tctccgtagt agcgccagct aagatgttcg ttttttgtgt aatagtagct tgcaccgttt
     2161 ttattttctt ggataaaata ttcatagatg taccgagcaa aaatgatgca ataaaataca
     2221 taagaattaa gccaggatat acaaatgccg aataaataat gacaaataca atgccgataa
     2281 cagcgacaaa aagtacattg ataaagtttt gcatgaagcg ttctgagtct tgccgcactt
     2341 tttgtaatat gtttaatgtt tcgccactgc gctggtcttc aaaagcttga aatggcaatt
     2401 gtagcgcgtg ttttagacca tctgtatata catctgcgcc aaatcgttgt atgacgacat
     2461 tcatgcaata atcttgaaat gctttggcaa tgcgactaat catggccaca gaaatagcac
     2521 atgcgattaa gaataaaaca ccttttataa attcagaaga agttctgcta aattgttttc
     2581 catctacaat ttttgtatgt ggatgtgttg cataatcatc gaatatctta ccaaatatgt
     2641 atggatctaa tagagaaaaa ctttggttga taatggcgag gataaatgtg agtaatacca
     2701 gccatttgta tctttgtaaa taatacagta aaattttcat tggaatacaa atgtacaaca
     2761 aatgaaaaat attgtttgtg aagtacaacg ttataaaata aaaaaacacg gcgattgccg
     2821 tgttttttgt tttagtatct cagatatact tatttgtcat tgtatgtata ggcaatacct
     2881 tttttcaaag attgattagc tgatgcgatg ctggcgatac cgccgcccca gccgccccag
     2941 ccgccgccgc tacttttgga ttcgctggtc ataaatacaa aaggacattt catttccgca
     3001 gcagcctcta acaatttttt attagctttt ttgtcagatt ttgaaccgct aaatccacct
     3061 gtgcttttac ctttgatttc atctactttt ttcaagccga caatttcaga cacatctagt
     3121 aagatttcta ctttttccca gtcttctttt ccgttgataa cgattttctc agaaataggc
     3181 tcttcacgac cacttgaata aattattttt tcgatggctt tttttgacat agtctgttca
     3241 gcatcttcac cttcatactt gaagataact ttgaagtcat ctactctgac gacttttcca
     3301 tcaattgttt ttccgctgtg taaaaaaatt tgatcgccag caaatacagc tatagcagca
     3361 aatgcaagca ttacagtagt tagtaacttt ttcatagaat acattgtttt agttaattaa
     3421 atttagtgtt ttttctttaa aatacaactt ttgtgccaaa ttgatttccg taactattta
     3481 ttttacaaag ctatttatct aatgattatt ttatatccgt gctaacacgt aaaatgtatt
     3541 tttttaaata gtataaaatt ttttggatat tttattctcc gaattgttta aattgtggaa
     3601 cttaataaaa caaactgacg ttttggtttt atattattca cttaaaaaat cataaattat
     3661 gttgtatgca ttaattggct taatcgtatt aggcattttc ttatcttttg taaccgtgca
     3721 acaaggcaca attgcggtca tcactatgtt tggtaagtac caacgtattt taggtccagg
     3781 cttaaatatg aaaattccat ttttcgaaca agtgtacaaa cgtgtttcta ttcagaaccg
     3841 ctcagtagaa ttggagtttc aggcaattac tattgaccaa gctaacgtat attttaaagc
     3901 aatgttgcta tatgcagtag tcaatactga tgaggcgact attaaaaatg tagcgtttaa
     3961 att
//
LOCUS       DAOQQV010000164         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_10987_length_3958_cov_8.036638, whole genome shotgun sequence.
ACCESSION   DAOQQV010000164 DAOQQV010000000
VERSION     DAOQQV010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10987_length_3958_cov_8.036638"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PLL59_11825"
     CDS             complement(<1..1349)
                     /locus_tag="PLL59_11825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMY24543.1"
                     /translation="MKKLSYFILISVLMIACSTSNKKQAANTSELQKDTIPQECIDPV
                     AIEFLGIDVDSLCVKEKELNRRDNLQKLFRTLKLDKYQYAQFIQDTKDYTDFTHITSR
                     QKYFTITKETDSSSLLQYLIFKISDFEYIITNCTDTSYVPFNKVQEDIVVLSKKKKKK
                     TKKIIPILYHPLQVAVYEKKIDTTAIKFAAVIQKSVAKTFKEHHIDPKIIEKLSKAYN
                     GKFNVANLRKGDTLQFVYDQLSINGRFLDYGHVQAICCKTKKKQYYAVDYFIYDDSSY
                     QYTDENGKYLKTAFIKSPLQKGGSIVSRFNLHRFHPVLQVEKAHLGTDFAAPQGTPIV
                     ATASGIVIAAQFTQNNGNYVKIRHDKVYETQYLHMSRFARGIRNGVRVQQGQVIGYVG
                     STGLATGPHVCYRFWKYGAQVDPLKEKLNVVKQIPKNDMSLYLQFFIVAKNILEKIP"
     gene            complement(1483..2601)
                     /locus_tag="PLL59_11830"
     CDS             complement(1483..2601)
                     /locus_tag="PLL59_11830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMY24544.1"
                     /translation="MKSLIVYSIPAFFVLIAVEIIIDKIKKTNLYRLNDTISNINTGI
                     VQQVTGVLLKLLNIGVYIWIYENYRIASIPNTWYTWILLLIGVDFFYYWFHRLSHEIS
                     VLWGTHVVHHQSEEYNLSVALRQSATQVFGSFWFYLPLAFFGFDPLSFIVISQIQTLY
                     QFWIHTRLIGKLPSWFEFVFNTPSHHRVHHGINPKYIDKNHGGTFIIFDRIFGTFQKE
                     EEEVHYGITSQLKSWNIAVANFDYYGWILQQIVKVKNIKDFFFVLLKPPGWRPDYLGG
                     AIVPKEIDKNRKLYDADARPNQHIYICIQFLTLLAFVSYFLFGIAKFDIYQQLAGITL
                     IVLSTISIGTLFEYKVWPAYLEFIRLLLAIPVMFYLTS"
     gene            complement(2715..3032)
                     /locus_tag="PLL59_11835"
     CDS             complement(2715..3032)
                     /locus_tag="PLL59_11835"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HMY24545.1"
                     /translation="MPIIDPALKEKIPPFLRNKFFMLFAAYFIYLLFFSQNTLISQSR
                     LYMQLRDLEKEESYYKKEIATIKKEQRELFSGIDQMEKFARENYWMKRDSEDLYIFIE
                     SEK"
     gene            complement(3146..>3958)
                     /locus_tag="PLL59_11840"
     CDS             complement(3146..>3958)
                     /locus_tag="PLL59_11840"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMY24546.1"
                     /translation="KIDFQEFMIVPVGATTFSDGLRMGVEIFHNLKKVLKAKGYSTNV
                     GDEGGFAPEIKSNEEAIETVLKAIESAGYKPGDQVKIAMDAAASEFYDAKKKKYIFHK
                     SDNKILTSEEMVDYWAKWVKKYPIVSIEDGLAEDDWKGWKLLTETIGNKCQLVGDDLF
                     VTNVKRLSEGVKNNIANSILVKVNQIGSLTETIEAVDLAHRNRYTSVMSHRSGETEDS
                     TIADLAVALNCGQIKTGSASRSDRMAKYNQLLRIEEELGEEAYYPGKAYRFF"
BASE COUNT         1240 a          703 c          629 g         1386 t
ORIGIN      
        1 attggaattt tttccaaaat attttttgct acaataaaaa actgcaaata caaggacata
       61 tcattttttg gtatttgctt taccacattt agtttttctt tcaatggatc gacttgtgcg
      121 ccgtatttcc aaaagcgata acaaacatgt ggaccagttg ccaagcctgt actgcctaca
      181 taaccaatga cttgcccttg ctgtacgcgc acgccattgc gaatgccacg tgcaaatcta
      241 ctcatgtgta aatactgcgt ttcatacact ttatcatgtc ttatttttac gtagttgcca
      301 ttgttttgcg tgaactgtgc agcaatgaca ataccacttg ctgtggcaac aatcggtgtg
      361 ccttgtggtg cagcaaagtc ggttcccaaa tgcgcttttt ctacttgtaa aacaggatga
      421 aatcgatgca aattgaatcg cgaaacaatg ctgccgcctt tctgtaatgg tgattttatg
      481 aatgccgttt taaggtattt cccattttcg tctgtgtact gataactgct atcatcatat
      541 ataaaataat caactgcata atattgtttc tttttagttt tgcaacaaat agcttgcaca
      601 tgaccataat ctaaaaatcg accattaata ctcaattggt catatacaaa ttgcagtgtg
      661 tcgcctttgc gtagattagc gacattaaat ttgccattat atgctttgct taatttctca
      721 attatttttg ggtcgatatg atgctcttta aatgttttcg ctactgattt ttgaataact
      781 gctgcaaatt ttatcgctgt cgtgtctatt ttcttttcat acacagcaac ctgcaaagga
      841 tgataaagaa ttggaattat tttttttgtt tttttctttt ttttcttaga caatacgaca
      901 atatcttctt gcactttatt aaacggtaca tagcttgtat ctgtacagtt ggtaatgata
      961 tactcaaaat cagaaatctt aaaaattaga tactgtaaca acgacgaact gtctgtttct
     1021 ttggtgatgg tgaagtactt ttgtcggctg gtgatatgtg taaaatcagt ataatctttt
     1081 gtgtcttgaa taaattgagc atattgatat ttatccaatt ttagcgtgcg aaataatttc
     1141 tgcaaattat ctcttctgtt taattctttt tcttttacac ataaactatc tacatcaatt
     1201 cctaaaaatt caatggcaac tgggtcgata cattcttgtg gaatggtgtc tttctgtagc
     1261 tcagaagtat tggctgcttg ttttttattt gacgtagaac aagctatcat caagacagaa
     1321 attaaaataa aataggatag ctttttcatg ttgggtttgc aataatatat ttcgttgaaa
     1381 tcaaagattt cattagataa ttagataaaa aaattgttat atgcggcgtt taataaaata
     1441 ctaagataca aaataaatag caaaaaagaa aaactgtctg tgttaggaag taaggtaaaa
     1501 catcacagga atagccaaca acaacctaat gaattctaaa taagctggcc aaactttata
     1561 ttcaaacaaa gtaccgatag aaatagtgga caaaacaatt aatgtgatgc ctgctaactg
     1621 ctgataaata tcaaacttgg caataccaaa taagaaatag gaaacaaatg ccaacaaagt
     1681 taaaaactgg atacaaatat aaatatgttg attaggtcgt gcatctgcat catataactt
     1741 tctgttttta tcgatttctt ttggcacaat tgcaccacct aaatagtcag gtcgccagcc
     1801 aggtggtttt aacaacacaa aaaagaaatc ttttatattt ttcaccttaa ctatttgctg
     1861 taaaatccag ccataataat caaaattagc cactgcaata ttccaacttt ttaattgcga
     1921 agttattcca taatgtactt cctcttcttc cttttgaaag gtgccaaata ttctgtcaaa
     1981 aattataaat gtgcctccgt gatttttatc aatatatttt ggattaatgc catgatggac
     2041 acgatgatgt gatggcgtat taaacacaaa ttcaaaccac gacggaagct tgccgattag
     2101 gcgcgtatgt atccaaaatt gatatagtgt ttgtatttga gaaattacga taaatgaaag
     2161 tggatcgaag ccgaaaaatg ccaatggtaa ataaaaccaa aaagagccaa atacttgcgt
     2221 agctgattgt cgcaaagcta ccgataaatt atattcttcg ctttgatgat gtacgacatg
     2281 cgtgccccaa agtacgctga tttcgtgcga taagcgatga aaccaatagt agaaaaaatc
     2341 aactccaatt agcaataaaa tccaagtgta ccacgtgttc ggaatgcttg caatacgata
     2401 attttcataa atccaaatat agacaccaat atttaacaac ttgagcaaca cacctgttac
     2461 ttgctgcaca atgcctgtat ttatattact tattgtatcg tttaagcgat acaagtttgt
     2521 cttttttatt ttatcaatga ttatttctac agcgattaaa acaaaaaatg caggtataga
     2581 ataaacgata agtgatttca tgcaaatagt tatgttattt gttataaaaa gtaacacact
     2641 taatatccat aaatatagtg ttttaggctg atgtatttac aaataatggc atctataaca
     2701 cagcgatcgt cgaattattt ttccgattct atgaaaatat ataaatcttc gctatcgcgt
     2761 ttcatccagt agttttcgcg cgcaaatttt tccatttgat cgatgccaga aaacaactcg
     2821 cgttgctctt tttttattgt ggcaatttct tttttgtaat aagattcttc tttttctaaa
     2881 tcgcgcaatt gcatgtataa ccttgattgc gaaatcaagg tgttttgaga gaaaaacaat
     2941 aagtaaataa aataagctgc aaataacata aaaaacttat tccgcaaaaa cggaggaatt
     3001 ttttctttta atgctggatc tattattggc atgttgtaaa agtagtaaga tatcggcaat
     3061 tggcctatct tattttgtaa acttttgtgt attaaaaaac gcctcacaat aattgtaagg
     3121 cgtttgtgtt tatctttaaa atattttaaa agaagcgata tgcttttcca ggataatatg
     3181 cttcttcgcc taattcctct tcgatacgta gcaattggtt gtattttgcc atacggtcgc
     3241 tgcgtgatgc agagccagtt tttatttgtc cgcaatttaa tgcaacagcc aaatctgcga
     3301 tcgtgctatc ttcggtttcg ccacttctat ggctcattac ggaagtatat ctatttctgt
     3361 gtgctaagtc aactgcttca atggtttctg tcaaagagcc tatttggttt acttttacca
     3421 aaatagaatt agcgatatta ttttttacgc cttcacttaa gcgttttaca ttggttacaa
     3481 ataaatcgtc gcctactaat tggcatttat ttccgatggt ttctgtcaac aatttccagc
     3541 ctttccaatc atcttctgct aagccatctt cgatagacac aattggatat ttttttaccc
     3601 attttgccca ataatctacc atttcttcac ttgtcaatat cttattgtct gatttatgga
     3661 atatgtattt tttcttcttc gcatcataga attcagaagc agctgcatcc atggcaattt
     3721 ttacttgatc gcctggtttg tagcctgcac tttcaatagc tttcaacacg gtttcaatgg
     3781 cttcttcgtt agattttatt tctggagcaa atccgccttc atcaccaacg tttgttgaat
     3841 atccttttgc ttttaatact tttttcagat tatgaaaaat ctccacaccc attctcaagc
     3901 catcagaaaa tgtagttgca ccaacaggca caatcataaa ttcttgaaaa tcgatttt
//
LOCUS       DAOQQV010000165         3904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_11221_length_3904_cov_10.230969, whole genome shotgun
            sequence.
ACCESSION   DAOQQV010000165 DAOQQV010000000
VERSION     DAOQQV010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11221_length_3904_cov_10.230969"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..524)
                     /locus_tag="PLL59_11845"
     CDS             complement(18..524)
                     /locus_tag="PLL59_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMY24547.1"
                     /translation="MEFDIIKSLVNEYTHECASTRKMLERVPMDKLDWQPHEKSMKLE
                     MLATHIAEIPSWVSSVINLSELDFAKTPYVPQKVSTAEALLKIHDEATTDALLSIKNT
                     NADVLKNETWTMRNGDIIYFTMPKIAVLRTWAFNHLYHHRGQMSVYLRLLNIPVPGIY
                     GPSADEQG"
     gene            669..1925
                     /locus_tag="PLL59_11850"
     CDS             669..1925
                     /locus_tag="PLL59_11850"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMY24548.1"
                     /translation="MKRVLITLHIMVSVVFFTYCQQHSNKTSTGFIQPEVNPALYRNK
                     DNEKINKQDTDIIQKIHALDCYFEDKVKTNAFNGAVLISYKGTPIFERYYGYKNYYTF
                     ERLTPSATFQIASTTKTFTSGAILLLLQQNMLSLEDSLEKYFPNFPYKGITIRMLLNH
                     RSGLPNYLNFAEQYWKNKKEFMSNADMLHLLYKYRPKALNAPNTHFRYNNTNYVLLAL
                     IVEIVSGQPFCDFLHQNIFMPLGMNDTWLYDPKSPWPSATKGYKGSRWIEDEIVYTDG
                     VTGDKGIYSTVRDLYKWDQALYSNKFIKTEILGLAFTPQSFEKAGNKNYGLGWRINDQ
                     TDGSRLIYHNGWWHSYNSVFNRKISDRSSVIILSSHYSSSVYKIQSIWDILYGAGVMS
                     NTEEDGSSDTTKKLNSDTLLEIQRSK"
     gene            complement(2095..3231)
                     /locus_tag="PLL59_11855"
     CDS             complement(2095..3231)
                     /locus_tag="PLL59_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24549.1"
                     /translation="MNKAVIKIKFPYLQRMLDKARSMFKIDMQKLRSADSSHLFWMDM
                     TMLIIVIINLLYLFFGFLFQFNAIASGIHYVLPRFHDWYAENVFEHVFHYDLIFVGIY
                     IAELLYRWIRSIYRKKYDKWWFYPFVHWYDVIMCIPIIKGFNFFAVVRLFGLVYRLQR
                     LGVFDLTKTYVFKKSAFVSDVVVEEVADRVIAKMISMAQEEVQKGSPLMEKIVSNVVK
                     PKEAEIVDYLSDRVGQAINISYTQYRSELQEYLFKKLKAAIHENQELKTLNYIPGIGN
                     FIKETLDRAVADITFNTIDKIMLDIIAPDNTRGVKELTHGIIESFLDRSHPANQDLNQ
                     IIVGIVHDSLEEIKQSVLMKEWKIEQAKVKKAKIEEEIRESQNI"
     gene            3465..3794
                     /locus_tag="PLL59_11860"
     CDS             3465..3794
                     /locus_tag="PLL59_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMY24550.1"
                     /translation="MKLNIKFIIVENKIQYPIETFEGEYRNLMALINDKIYPDYFGEC
                     GGLGRCATCLVKVEGLSGVSVKKERNEPVTLLKNGLTDENIRLSCQLLISKDLDGTIV
                     EVVEYNQ"
BASE COUNT         1312 a          660 c          645 g         1287 t
ORIGIN      
        1 tttactataa aaattaatca gccttgctcg tctgcggaag gtccataaat tccaggaact
       61 ggaatattca ataatcttaa atacacagac atctgaccac gatggtgata taaatgattg
      121 aatgcccaag tgcgcaaaac tgcaattttt ggcatcgtaa aatagataat atcgccattg
      181 cgcattgtcc aagtctcatt ctttaataca tcagcattcg tattttttat agacaacaaa
      241 gcatcagtag ttgcttcatc atgtattttc aataaagctt cggcagtgct tactttttga
      301 ggaacataag gtgtttttgc aaaatctagt tctgataaat taatgacaga agaaacccaa
      361 cttggaattt ctgcaatatg cgttgccaac atttctagtt tcatcgactt ttcatgtggc
      421 tgccaatcta atttgtccat cggtactcgt tcgagcatct ttctggtgct agcacactca
      481 tgtgtgtact catttaccaa gcttttaatg atatcaaatt ccataacttt ttttgtaaaa
      541 ataaggaaaa accatttttc aacgctatgg taacatctat tttataacat ttatttacaa
      601 atcatctatt tttatggcga ttgaattgta ttatgttggt agcccttacg aagaagcaag
      661 taagaatgat gaaaagagta ttaataacgt tacatataat ggtgtctgtg gtatttttta
      721 cctattgcca acaacattcc aataaaacaa gcacaggttt cattcagcca gaagtaaatc
      781 ctgcattgta tcgcaataaa gacaatgaaa agattaataa acaagacact gacattatcc
      841 aaaaaatcca tgctttagat tgttattttg aagataaagt aaaaacaaat gcttttaatg
      901 gcgctgttct catttcttat aaaggcacac ctatttttga acggtattat ggttataaaa
      961 attactatac ttttgagcga ttaacgccga gtgcaacatt ccaaatagca tccaccacca
     1021 aaacttttac ctctggtgct attttattac tgctacaaca aaacatgttg tctttagaag
     1081 attctttaga aaaatatttt cccaattttc catataaagg cattaccatc agaatgctat
     1141 taaatcatcg ctctggcttg cctaattatt taaactttgc tgagcaatat tggaaaaaca
     1201 aaaaggaatt tatgtcaaat gcggatatgc tacacttatt gtataaatac agaccaaaag
     1261 cattgaacgc tccgaatacg catttccgat ataacaatac aaattatgtg cttttagcat
     1321 tgatcgtaga aattgtttct ggacaaccgt tttgcgattt cttacatcaa aatatattta
     1381 tgcctttagg catgaatgat acttggctat atgatccgaa aagtccatgg ccatcagcca
     1441 caaaaggcta taaaggctcg cgctggattg aagatgaaat cgtttatact gatggcgtaa
     1501 ctggcgacaa aggtatttac agcaccgtta gggatttgta caaatgggac caagcattgt
     1561 attccaataa attcattaaa acagaaatat taggactggc atttacgcca caaagttttg
     1621 aaaaagcagg caataaaaat tatggtttag gttggcgcat taacgaccaa actgatggct
     1681 caaggttgat ttatcacaat ggctggtggc atagctataa ttctgttttt aatcgcaaaa
     1741 taagcgatag atcttctgta attatactga gtagtcatta tagttctagt gtttacaaaa
     1801 tccaatctat ttgggacatt ttatacggtg ctggtgttat gtcgaacacc gaagaagatg
     1861 gaagcagcga caccacaaaa aagctaaatt cggatacttt gctagaaata cagcgttcta
     1921 aataatagga gtagattgct ccaaagtcga aatagatata aaataaaaaa tcccgggcaa
     1981 ccacgcaccg ggactatttt aaaccaacca aatgaaaata ttttttaact tttaactatg
     2041 ttttttagtt attaattaag taacaattct cgtgccaaag tggttttttg ttggttaaat
     2101 attttgtgat tctcgaattt cttcctcaat ttttgctttt tttacttttg cttgttctat
     2161 tttccattct ttcatcagca cgctttgttt tatttcttcc aaagaatcgt gtacgatgcc
     2221 tacaataatt tggtttaaat cttgatttgc tggatgtgaa cgatctaaaa atgactcaat
     2281 gatgccgtgt gtaagctctt ttacgcctct ggtattatca ggcgcaatga tgtcgagcat
     2341 gattttatca atcgtgttaa aggttatatc cgcgacagca cgatccagtg tttcttttat
     2401 aaaattgcca attccaggaa tataatttaa ggtttttagt tcttggtttt catgaatggc
     2461 tgctttcagc tttttaaata aatattcttg taattcactc cgatattgtg tgtaggaaat
     2521 attaatcgcc tgcccaacac ggtcgctcaa ataatcgaca atttctgctt cttttggttt
     2581 aaccacattt gacacaattt tttccattaa aggtgagcct ttttgcacct cttcttgcgc
     2641 cattgatatc atcttagcaa tcacacggtc ggcaacttct tccactacca catctgatac
     2701 aaatgccgat tttttgaaaa cataggtttt agttaaatca aaaacaccta agcgttgcaa
     2761 acgataaacc aatccgaaca atcttacaac agcaaaaaaa ttaaatcctt tgataatagg
     2821 aatacacatg attacatcat accaatgtac aaatggataa aaccaccatt tatcatattt
     2881 ttttctgtaa atactgcgta tccaacgata tagcaattcg gcaatataga tgccaacaaa
     2941 aattaggtcg taatgaaaga cgtgttcaaa tacattttcg gcataccaat cgtgaaagcg
     3001 tggcaaaaca taatgaatac ctgatgcaat tgcattgaac tgaaacaaaa atccaaaaaa
     3061 caaataaagc agattgatga tgacaataat caacatggtc atatccatcc aaaacaaatg
     3121 agatgaatca gcagagcgta atttctgcat gtcaatctta aacatgctac gtgccttgtc
     3181 taacatgcgt tgaagatagg gaaattttat ttttatgact gccttattca attacataag
     3241 gacttatggt actacaaaaa taggataaca caatcattta attatataat atgaataaag
     3301 agtggtgtta taatgctaaa tattgtttcg tgttctcacg aaacaaggcg aagagcattc
     3361 caatatcaat tattaaatat aaaattgtgg ttgaatttgt ttttaattta gtaagatatt
     3421 tttatcgttg tattttatag ctttgatggg attattattc accaatgaaa ttgaacataa
     3481 aatttattat tgttgaaaac aagattcaat atcctattga aacatttgaa ggtgagtata
     3541 gaaatttaat ggctttaatc aatgataaaa tttaccctga ttattttggt gaatgcggtg
     3601 ggcttggtag atgcgcaact tgtttggtta aagtagaagg attgtcgggt gtgtcagtaa
     3661 aaaaagaaag aaacgaaccc gtaactttat taaaaaatgg acttacagat gaaaacattc
     3721 gtttgtcttg ccaattatta ataagcaaag atctagacgg tactattgtg gaagtagttg
     3781 aatataatca ataaatttct aatgccttgt ttcgtatctc cacggaccta atgccatggt
     3841 tcgtgtctcc accataagtg ttcggaagat tacgttgtat tataagaata gagaaattga
     3901 ttta
//
LOCUS       DAOQQV010000166         3864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_11398_length_3864_cov_6.400630, whole genome shotgun sequence.
ACCESSION   DAOQQV010000166 DAOQQV010000000
VERSION     DAOQQV010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11398_length_3864_cov_6.400630"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..626
                     /locus_tag="PLL59_11865"
     CDS             420..626
                     /locus_tag="PLL59_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24551.1"
                     /translation="MSTLILKTTSKKKLDLIEALAKELGISIQKETSRTKVPNSTTLN
                     AIKEAKTGKSTKVTLGAFRKQLYI"
     gene            743..901
                     /locus_tag="PLL59_11870"
     CDS             743..901
                     /locus_tag="PLL59_11870"
                     /inference="COORDINATES: protein motif:HMM:NF027074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HMY24552.1"
                     /translation="MPANFKPYFLKGNFKELWECHIQPDWLSVWEQNETIKLITLYRT
                     GTHSDLFK"
     gene            1026..2468
                     /gene="miaB"
                     /locus_tag="PLL59_11875"
     CDS             1026..2468
                     /gene="miaB"
                     /locus_tag="PLL59_11875"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HMY24553.1"
                     /translation="MNELLDYIEQKKHDENRQGEALNLALNESEFKNNKHFYIESYGC
                     AMNFSDSEIVASILAKQGYGITTNIFDADLILVNTCSIREKAEQTVRQRLRTFNQIKE
                     QKPSLMVGVLGCMAERLKSKFLEEEKLVDIVVGPDAYRSLPELLHEAEGGQKGINVLL
                     SREETYADISPIRLDKNGITGFISIMRGCDNMCSFCVVPFTRGRERSRNPETIVEEAR
                     DLFNRGYREVTLLGQNVDSFKWKGEGKNDDKEAAILVNFAQLLEMVALVNPLLRVRFS
                     TSHPKDITDDVLHTIAKYNNICSYIHLPVQSGSSSCLERMNRTYDRDWYMNRVDAIRR
                     IIPDCGISSDVIAGFCDETEEEHLETLSMMDYAQYDYSYMFYYSERPGTLAARKYTDN
                     VPLETKKRRLAEIVEKQYQLSLQNNLKDIGKTYEVLVEKISKKNEAEYCGRNSQNKMI
                     IFPKGNYNIGDYVNVYIESANKATLKGRVV"
     gene            2572..3813
                     /locus_tag="PLL59_11880"
     CDS             2572..3813
                     /locus_tag="PLL59_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMY24554.1"
                     /translation="MDIQSVKQRFGIIGNAPALNRALEVAVRVAPTNLSILITGESGV
                     GKEVFSQIIHNLSTRKHENFISVNCGAIPEGTIDSELFGHEKGAFTGAHDKRKGYFET
                     VNGGTIFLDEIGEMPIGTQARLLRLLENGEFIKVGSSIVQKTDVRVLAATNVDLPKLI
                     KQGKFREDLYYRLNTVPIFVPPLRERKEDIVMLFRKFASDFSEKYRMQAVQLSEDAKQ
                     ILISYRFPGNVRELKNIAEQVSVLSKEKTIGKEELARFIPTVEQNTLPTITHNNETTS
                     FTDRELLYKMIFDIKHDLNDLKSFVWNMSNGNFDDTKIDFSDVSGIEKNISNTTPNVM
                     SKQYPVIENTSGNPTIILNDEPQYEEESIDSSLSLADMEKSMIIKALDKHKGKRRDAA
                     LDLGISERTLYRKIKEYKLEE"
BASE COUNT         1425 a          566 c          695 g         1178 t
ORIGIN      
        1 ggctgttcta attgttgttg tatagtaaaa tcatcaccat ctaagaaaag ataattgcct
       61 ttttcagcaa taatttcttt tatcaatgtg gatttacctg tttgtcgcgc tccaattaat
      121 acaattgcct ttccgatatt gaataaatca actaaattag attttattat tcgttgcatt
      181 ttcatagggt aaatatagta attttttatc aaaacagcca aagattatat tatttatatg
      241 cttaaacagc taaatattaa actattttac cgccaggata tctgaattca tattactgat
      301 aattccgaaa aggaaaattt taagaaattt taagggaaaa ttgcattacg ataatcgtca
      361 aatcttgttg aactttaatg atatttttgt attttttcta ttaataaata ctaagttaaa
      421 tgtcaacgtt aatattaaaa acaacatcga agaagaagct tgatttgata gaagcattgg
      481 ctaaagaatt aggaataagt attcaaaaag aaacttcaag aacaaaggta ccaaattcta
      541 ctactttaaa tgctattaaa gaggctaaaa caggaaaatc taccaaagtg acattaggtg
      601 cttttagaaa acaattgtac atataatgta tcaattagaa tttgtcaatc aatttaaaaa
      661 agatttgaaa ctcgcaaaga aacgtggttt aaatatggaa ttattagaca atgtcgttac
      721 cttataagtt actaatggaa aattgcctgc taattttaaa ccatatttct taaaaggtaa
      781 ttttaaagaa ttatgggaat gccatattca accggattgg ttgtcagttt gggaacaaaa
      841 tgaaactatc aaattaatta cactatacag aacaggtact cattctgatt tatttaagtg
      901 atttattcta cttttaaaac caactaaagc atattttaat ttactttttc agtagattaa
      961 ctataaaatc tctctgccaa tttgtcactt cggcttattt ttcctatctt tgttgtccat
     1021 ttcttatgaa cgagttatta gattatattg agcaaaaaaa acacgatgaa aatcgtcaag
     1081 gcgaagcgct aaatctagcg ctgaatgaaa gtgaatttaa aaacaacaag catttttata
     1141 tagaaagcta tggttgtgct atgaacttta gcgacagcga aatagtagct tctatacttg
     1201 ccaaacaagg atacggcatc acgacaaata tttttgatgc tgatttaata ttagtaaaca
     1261 cttgttctat tcgcgaaaaa gcagaacaaa ccgttcgtca gcgcttgcgt acatttaatc
     1321 agataaaaga acaaaagcca tcgctaatgg ttggtgtctt aggttgtatg gcagagcgtt
     1381 taaaatcaaa atttttagaa gaagaaaaac tggtagatat agtagtgggt ccagacgctt
     1441 atcgatcatt accagaatta ttacacgaag cagaaggcgg acaaaaaggt attaatgttc
     1501 ttttatctcg agaagaaaca tacgcagaca tctctcctat tcgcttagat aaaaatggca
     1561 ttactggatt tatttccatt atgcgtggct gtgacaacat gtgttcgttt tgtgtggtgc
     1621 cttttactcg cggcagagag cgcagcagaa atccagaaac aattgtcgaa gaagcacgcg
     1681 acttatttaa tcgcggatat cgtgaggtga ctttgcttgg acaaaatgta gattctttta
     1741 aatggaaagg tgaaggaaaa aatgacgata aagaggctgc aatattggta aattttgcac
     1801 aattattaga aatggtggca ttagtcaatc cattattgcg tgttcgtttt tcaacttcac
     1861 atccaaaaga cattacagat gatgtgttgc atactattgc taaatacaat aatatttgca
     1921 gctacataca cttgccggta cagtcaggca gctcatcctg cttagagcgt atgaaccgta
     1981 catacgacag agattggtat atgaatcgag ttgatgcgat tagaagaatt attccagatt
     2041 gtggtatttc atcagacgta atcgctggtt tttgcgacga aacagaagaa gaacatttag
     2101 aaacattatc gatgatggac tatgctcaat atgactatag ttatatgttc tattactctg
     2161 agcgacctgg cacattagca gcaagaaaat acacagacaa tgtgcctttg gaaacaaaga
     2221 aaagaagatt ggcagaaata gtagaaaaac aataccaact ttctttgcaa aacaatttaa
     2281 aagacattgg taagacatat gaagtgttag tagaaaagat ttctaagaaa aatgaagcag
     2341 aatattgcgg cagaaattct caaaataaga tgattatttt tcctaaagga aactacaaca
     2401 ttggcgacta tgttaatgta tatattgaaa gtgctaataa agcaacattg aaaggaagag
     2461 ttgtttaatg aaataatgta tcaatgtaat aattaactaa tgaagtaacg ttcagttttc
     2521 atcaaattac attgttacat aactacattt taaaatttat caaattaagt aatggatata
     2581 caatcagtaa aacaacgctt tggaataatt ggcaatgcac ctgcactcaa tagagcattg
     2641 gaagtggctg tgcgtgtagc gccaaccaac ttgtcgatat tgattactgg tgaaagtggc
     2701 gttggtaaag aagtattttc acaaatcata cataacttat cgacacgtaa gcacgaaaat
     2761 tttatttctg tcaattgtgg tgccatacct gaaggaacta tcgattccga gttatttgga
     2821 catgaaaaag gagcatttac tggcgcacac gataagcgta aaggttattt cgaaacagta
     2881 aatggtggca ctattttttt agatgaaatt ggtgaaatgc ctattggcac acaagcgcgt
     2941 ttattgcggt tgctcgaaaa tggagaattt ataaaagtgg gttcttctat agtgcaaaaa
     3001 actgatgtgc gcgttttagc agcgacaaat gtagatttgc caaagttgat aaagcaagga
     3061 aaatttagag aagatttata ttaccgtcta aatacagtgc cgatttttgt accacctttg
     3121 cgcgaacgaa aagaagacat cgtgatgttg tttagaaaat ttgcatctga tttttcagaa
     3181 aaatacagaa tgcaagccgt tcaactttct gaagatgcaa aacaaatttt aatcagctat
     3241 cgctttccag gtaatgtgcg tgagctgaaa aatattgcag aacaagtttc tgtattatcg
     3301 aaagaaaaaa caattggcaa agaagaattg gcaagattta taccgacagt agaacaaaat
     3361 actttgccaa caattacaca caataatgaa acaacatcat ttacagatag agaattattg
     3421 tacaaaatga tattcgatat caaacacgat ttaaatgact taaaaagttt tgtgtggaat
     3481 atgagcaatg gaaattttga cgacaccaaa atcgatttca gcgacgtttc tggtattgaa
     3541 aaaaacataa gcaacactac gccaaatgta atgtctaaac aatatcctgt tattgaaaat
     3601 actagtggca atccaacaat tatcttgaat gatgaaccac aatacgaaga agaaagtata
     3661 gactccagtt tatcgttagc agatatggaa aaaagtatga ttataaaagc tttggacaaa
     3721 cacaaaggca aacgtcgtga tgcggctttg gatttaggaa tttctgaacg cacattatac
     3781 agaaaaatta aagagtataa attggaagaa taatgaatgt agcaatgtaa taatgtagta
     3841 atgtagtaat gtaataatgt aata
//
LOCUS       DAOQQV010000167         3833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_11531_length_3833_cov_6.984383, whole genome shotgun sequence.
ACCESSION   DAOQQV010000167 DAOQQV010000000
VERSION     DAOQQV010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11531_length_3833_cov_6.984383"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLL59_11885"
     CDS             <1..380
                     /locus_tag="PLL59_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24555.1"
                     /translation="EYDYIIDAIDSITPKINLLKASHQLKKKIIMSGGAGGKLDPTKL
                     MVADLSKTYNCVFVKTVRKRLKKEKIYKGIKVVFSSELPDRDSLIMTDGANYKKSAYG
                     TISYLPAAFGGVCASVVIRDLMQ"
     gene            complement(414..896)
                     /gene="purE"
                     /locus_tag="PLL59_11890"
     CDS             complement(414..896)
                     /gene="purE"
                     /locus_tag="PLL59_11890"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HMY24556.1"
                     /translation="MKKIAIIMGSDSDSPVMQEAAKVLTDFGIEFEYTIISAHRTPER
                     MYEFARTAHEQGVEVIIAGAGGAAHLPGMVAAISPLPVIGVPVQSKALSGLDSLLSIV
                     QMPGGVPVATVAINGAKNAGILAAQIIAAHDKSLLQKIIDYKKNLEKEVIEKTKNFHP
                     "
     gene            complement(961..2112)
                     /locus_tag="PLL59_11895"
     CDS             complement(961..2112)
                     /locus_tag="PLL59_11895"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HMY24557.1"
                     /translation="MQTINAIGIIGGGQLGKMFIENASKLAIPINILDKDANCSCSKL
                     ATQHILGNITDADDIEKLSAISDVLTWEIEHINVDKLIALQEQGKTIIPNPLVLKIIQ
                     DKGKQKTFYKENNIPTADFFIANNREEAKTKLAQSNFKKVAVKSCTGGYDGKGVFIAD
                     VTSILENEIDIPFDGNLMIEEFIACKKELAVLVARDIHGNNAIYPAIEMEFADSNLVS
                     FLISPANIDSTIEQKAQQIATQCADAFNSAGLFAIEFFLSHNDELFVNEIAPRPHNSA
                     HHTIEGFYTSQYEQLLRVLLGLPLGSTEIIEPCAMVNIVGDKTGIYKLTYLNELAKLQ
                     GVYVHLYGKKESRPNRKLGHITILHKDRKELIQLAEKVRTLAEIEIIEK"
     gene            complement(2200..2781)
                     /locus_tag="PLL59_11900"
     CDS             complement(2200..2781)
                     /locus_tag="PLL59_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMY24558.1"
                     /translation="MKKITVYCGANAGNEIVFEEQAFLLGKTLAQQQIGLVFGGGKVG
                     LMGIVANGAIQHGGEVIGVIPHFLCTKEIAHDGVTEMISVDTMHQRKTILYELSDAMI
                     ALPGGFGTMEELFEMLTWAQLGLHHKPIALLNTNGFYDALLAFIQTMVDTQFLKKEYQ
                     QMLLHSDNIDDLLEKIRNYEAPKVTKWINKENT"
     gene            complement(3055..3258)
                     /locus_tag="PLL59_11905"
     CDS             complement(3055..3258)
                     /locus_tag="PLL59_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24559.1"
                     /translation="MATSKKQDKEPVRKLTAHDLAFGIGRKFTDEEIQLLIDEHKDDV
                     FIDGDLAVKNIIKKLNKKKVAKS"
     gene            complement(3376..3513)
                     /locus_tag="PLL59_11910"
     CDS             complement(3376..3513)
                     /locus_tag="PLL59_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24560.1"
                     /translation="MKKITLYCGANAGNEIVFKEQAFLLGKTLAQQQIGLSPCFVSHE
                     T"
     gene            complement(3522..>3833)
                     /locus_tag="PLL59_11915"
     CDS             complement(3522..>3833)
                     /locus_tag="PLL59_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HMY24561.1"
                     /translation="EQEFDVMLCWMNEKKMVARGKYIIRHTSKECKCIVKEAKYKLDI
                     NTLQRIEDDVEIGLNDIGRVTLRTTVPLFFDSYRKNRITGSIILVDEATNETVAAGMM
                     V"
BASE COUNT         1127 a          701 c          650 g         1355 t
ORIGIN      
        1 atgagtatga ttatatcatt gatgcgattg acagcattac gccaaaaata aacttgctga
       61 aggcatcaca tcaactaaaa aagaaaatta ttatgagtgg tggtgctggt ggcaagctcg
      121 acccaaccaa actaatggtt gcagatttgt ccaagacgta taattgtgtg tttgtgaaaa
      181 cggtacgtaa gcgactcaaa aaagaaaaaa tatacaaagg aattaaagta gttttttcta
      241 gcgagctacc cgacagagac tcattaataa tgaccgatgg tgccaattat aaaaaatctg
      301 cttatggtac catttcttat ctgccagcag catttggtgg cgtctgtgcg agcgtagtta
      361 ttagagattt gatgcaatag tttctgttga ggaaagaaaa tacagtagca aatttatgga
      421 tgaaaatttt ttgttttttc tatcacttcc ttttctaaat tctttttata atcgattatt
      481 ttttgcaata atgatttgtc gtgtgctgca ataatttgcg ctgctaaaat tccggcattt
      541 ttagctccat ttatggctac agttgccact ggcacaccac ctggcatttg cacaatagac
      601 aataaagaat ctaagccgct gagtgctttg ctctgcacgg gcactccgat aactggcaat
      661 ggcgaaatag ctgcaaccat gcctggtaaa tgcgcggcgc ctccggcgcc ggcgatgatt
      721 acttctacgc cttgctcatg tgcagtacgt gcaaattcat acatgcgttc tggcgttcga
      781 tgtgcggaaa taatggtgta ttcaaattca attccaaaat cggtcaacac ttttgcagct
      841 tcctgcatta caggtgagtc gctgtccgaa cccatgatga tggctatttt tttcatctta
      901 taccttttga attttatatc atttgttgta actcaaatct tacaacgtat tgcgattaac
      961 ttatttttca ataatttcta tctctgctaa agttcgtact ttttctgcta attgtattaa
     1021 ttcctttctg tctttatgca agatggtaat atgacctaat tttctgttgg gtcgactctc
     1081 ttttttacca tataaatgca catatacacc ttgtagtttt gctaattcat tgagatatgt
     1141 aagcttgtaa atgcctgttt tgtcgccaac aatattgacc attgcacagg gttcaataat
     1201 ttcggtagag ccgagtggca ggcctaataa aacacgcaat aattgttcgt attgtgaagt
     1261 gtaaaaacct tcgatagtat gatgtgctga gttgtgtggt cgtggtgcta tctcattgac
     1321 aaacaattca tcgttgtgcg ataagaaaaa ttcgatagca aataaacctg cgctattaaa
     1381 tgcatcggcg cattgcgttg caatttgttg tgctttctgt tcgatagtac tgtcaatatt
     1441 cgctggagaa ataagaaaag acaccaaatt gctatctgca aactccattt caatagctgg
     1501 atatatcgca ttattaccat gtatatcacg tgctactaaa acagctaatt cttttttaca
     1561 cgcaataaat tcttctatca tcaaattacc atcaaatgga atatcgattt cattttccag
     1621 aatagatgta acatcagcaa taaaaacacc tttgccatca taaccgccag tgcacgattt
     1681 aacggcaacc tttttgaaat tagattgtgc aagtttagtt tttgcttctt ctctgttatt
     1741 tgcaataaaa aagtcagcag ttggaatatt attttctttg taaaacgttt tttgtttgcc
     1801 tttatcttgt attattttta acaccaaagg attgggaata atagtttttc cttgttcttg
     1861 taatgcaatt aatttatcga cattaatatg ttcaatttcc caagttagta catctgaaat
     1921 ggcagatagt ttttcgatat catcagcatc tgtgatattg ccaaggatat gttgcgtggc
     1981 taatttacta caagagcaat tagcatcttt gtctaagatg ttgataggaa tagctaattt
     2041 tgaggcgttt tcgatgaaca tttttccgag ttgtccaccg ccgataatgc caatagcgtt
     2101 tattgtttgc attactgcaa agataagcat tgcattgtga attcagctat acgaatttgg
     2161 atttttactt tttgaaattg ataagaaacc tactgtttgt tatgtgtttt ctttatttat
     2221 ccatttagtg acttttggcg cttcgtagtt tcttatcttt tcaagtaaat catcaatatt
     2281 atcgctgtgt aacagcattt gctgatattc tttttttaga aattgagtat ctaccatcgt
     2341 ttgaataaag gctaataagg catcataaaa accatttgta ttcagcaatg cgattggctt
     2401 gtgatgtagc cctaattgtg cccacgtaag catttcgaat aattcctcca tggtgccaaa
     2461 tccacctggc aatgcaatca tggcatctga aagttcatac aaaatggttt ttcgttgatg
     2521 cattgtgtca acgctaatca tttccgtaac gccgtcatgt gcaatttctt tggtgcataa
     2581 aaagtgagga ataacgccga ttacttcgcc gccatgttgt atggcaccat tagcaacaat
     2641 acccattaag cctactttgc caccgccaaa tacaagaccg atttgttgtt gcgctaatgt
     2701 ttttccaagc aaaaaagctt gttcttcaaa gacaatttca ttgccggcat ttgcgccaca
     2761 gtacactgta atttttttca tttttaatac aagctcgatg ctaaaacaat ctgataaaca
     2821 ttgaattgtt ctttatcaac tttaaataca attatccaac tatcaatggc aacacaactg
     2881 tatccatttc ttgcataaac cttatgccgg caaatggcat attttattgg ttgtgcatat
     2941 ttcttaatct aattatgaaa tttaatcagc cacctttcac cagaaccagc agtgttcata
     3001 tcttctaaat aatctgctac tttggataat gagttattgg ctaatgaact ataattaact
     3061 tttagcaact ttctttttgt ttaatttctt tataatattt ttaacagcta aatcgccgtc
     3121 aataaaaaca tcatctttat gttcatcaat taataattga atttcttcat cggtaaattt
     3181 tctgccgatg ccgaatgcta aatcgtgagc agtgagtttg cgaactggtt ctttatcttg
     3241 ttttttagat gtagccataa tcaaatatag atataaatga gtttttgtgc aatgatattt
     3301 gtaaacaata attgattttc tcaatagagt tatacgcatt agaagatgca taaactctat
     3361 aatataaatt gtatattatg tttcgtgaga cacgaaacag ggcgatagac cgatttgttg
     3421 ttgcgctaat gttttaccaa gcagaaaagc ttgttcttta aagacaattt cattgccagc
     3481 gtttgcgcca cagtacaatg taattttttt catttttaat tttataccat cataccagca
     3541 gcaactgttt cattcgttgc ttcatctact aaaatgatgc ttcctgtgat tctgtttttt
     3601 ctataagagt caaaaaataa aggaactgta gtgcgcaagg taacacgacc aatatcattt
     3661 aatccgattt caacatcatc ttctatacgt tgcaatgtgt tgatgtcgag tttgtatttt
     3721 gcttctttta caatacactt acattcttta gaggtatgcc taataatgta tttaccacga
     3781 gctaccatct tcttttcatt catccagcac aacatgacat caaattcttg ttc
//
LOCUS       DAOQQV010000168         3819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_11605_length_3819_cov_8.413656, whole genome shotgun sequence.
ACCESSION   DAOQQV010000168 DAOQQV010000000
VERSION     DAOQQV010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11605_length_3819_cov_8.413656"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..375)
                     /locus_tag="PLL59_11920"
     CDS             complement(229..375)
                     /locus_tag="PLL59_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24562.1"
                     /translation="MGFSALSKTDRKAIVDGIIMDNKNVANIIGNVMPIINKLKKDEV
                     VPFF"
     gene            732..1067
                     /locus_tag="PLL59_11925"
     CDS             732..1067
                     /locus_tag="PLL59_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMY24563.1"
                     /translation="MQFHLGKKIREYAKLKGIKHTELAQRIITSKQNIQNIYRRESID
                     ILLLVKICNVLEYNFFTDYLAQLDFDVEKEIKEVLNVLKQSLQEKNKEIEYLKEIIEL
                     QKKLGKQEK"
     gene            complement(1072..1473)
                     /locus_tag="PLL59_11930"
     CDS             complement(1072..1473)
                     /locus_tag="PLL59_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HMY24564.1"
                     /translation="MAGTSKHQRLSFTKEERLKSRKRIDELFSEKAFVQNQLIRIYFK
                     QVELNCAFPAQFAFAAPKKIFPNAVDRNRLKRLMRESMRLQKPEFYKILQEKDIQIVL
                     LLSYHAKKTHDYKEVDEAVIKLLKKLVNELG"
     gene            complement(1473..1628)
                     /gene="rpmH"
                     /locus_tag="PLL59_11935"
     CDS             complement(1473..1628)
                     /gene="rpmH"
                     /locus_tag="PLL59_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HMY24565.1"
                     /translation="MKRTFQPSVRKRKNKHGFRERMATKGGRAVLNARRAKGRKKLSI
                     SDERKGK"
     gene            complement(1768..3252)
                     /gene="rho"
                     /locus_tag="PLL59_11940"
     CDS             complement(1768..3252)
                     /gene="rho"
                     /locus_tag="PLL59_11940"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HMY24566.1"
                     /translation="MQEQEIKEDESVKNLETDANDGAPKKEKKERTRIARKVAEKVVP
                     AKKTKENVDAENKQSAPHKKAEIQQSVIQEEVAAEVEEKIQEETHVEPSRESVADIIH
                     AIEQNDEVALPEEKKIDFNIDLDGVILGEGVLEIIPEGYGFLRSSDYNYLSSPDDVYV
                     SPSQIKLIGLKTGDTIVGNIRPPKEGEKYFALLRVESINGKTPQEIRDRVPFDYLTPL
                     FPTEKLDLFQEANNYSTRIVDLFTPIGKGQRGMIVAQPKTGKTMLLKDIANSIAKNHP
                     EVYLIILLIDERPEEVTDMQRSVKAEVVASTFDEPADRHVKVASIVLQKAKRLVECGH
                     DVVILLDSITRLARAHNTVAPSSGKVLSGGVEANALHKPKQFFGAARNIENGGSLTII
                     ATALTDTGSKMDEVIFEEFKGTGNMELQLDRKLSNRRIFPAVDVVASSTRRDDLLLDK
                     DALQKMWILRNHLADMNSEESMKFLLQQMKGTRSNEEFLLTMNR"
     gene            3618..>3819
                     /locus_tag="PLL59_11945"
     CDS             3618..>3819
                     /locus_tag="PLL59_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMY24567.1"
                     /translation="MLTLEQLRKDTTFAKERLAHKHIKDVHFVDEIIAFDDQRKTIKA
                     QVDDLLSQRNTLSKEIGALMKEG"
BASE COUNT         1157 a          673 c          582 g         1407 t
ORIGIN      
        1 ttattctatt catagattaa taaaatctat ctttttatat tctaattaac aaggaacata
       61 ttgcatacat aaattttccc cttggacttt acagtgatca gtgtaccaag atgaatattt
      121 tttgttataa tatccatacc aataatcgca aagtaaccaa tctgcatgat aggttgcaac
      181 tactctgtct ccatctttaa ttacaaagtt tgaattaggt attaagattt aaaaaaaagg
      241 aactacctca tccttcttta atttgttaat gattggcatt acatttccaa ttatatttgc
      301 tacattttta ttatccataa taattccatc tacaattgct tttctgtctg tctttgataa
      361 tgctgaaaaa cccataacac cttctttaat tttgcgttcc tcagcagcaa atgttgaatt
      421 tttatcaata tttaactctg tatcggtata cttatctgta tttgatacgt tctgttctac
      481 tttcctacat gcaataaata cgccaattac aaaatagagt tatggctaga attttcattt
      541 tcataaattt agtattttaa tttaattctt actcttttaa aggttttcgg aaatattcct
      601 ttttatgtct ttcgaaagat tcatatacaa atataaacaa aattgttgaa agtaaataat
      661 ttatttacta aagagttatt aatgataccg atttgattga aaagtaaaat ttctttgttc
      721 aactttagtt catgcaattc catttgggca aaaagataag agagtacgca aaattaaaag
      781 gtattaagca tacagaatta gctcagagaa taattacatc taaacaaaat attcagaata
      841 tatacagaag agaatccata gatattttat tattagtgaa aatatgtaat gtattagagt
      901 ataatttttt tacagactac ttagcacaat tagattttga tgtagaaaaa gaaataaaag
      961 aagtgttaaa tgtattaaag caatctttac aagaaaagaa taaagagatt gaatatttaa
     1021 aggaaattat tgaattgcaa aaaaaattag gtaaacagga gaaataataa atcatcctaa
     1081 ctcattaact aattttttca gcaactttat tactgcttca tccacttctt tataatcgtg
     1141 tgtttttttt gcatgatagc tcaatagcaa aactatctgt atgtcttttt cttgaagtat
     1201 tttgtaaaac tcgggttttt gtaagcgcat gctttcgcgc atgagtcttt ttaaacggtt
     1261 tctatctaca gcatttggaa atattttctt aggtgctgca aaagcaaatt gcgccggaaa
     1321 ggcgcaattc agttctactt gtttaaagta tattctaata agttggtttt gcacaaaggc
     1381 tttctcgcta aaaagttcgt ctatgcgttt tctgcttttt agtcgttctt ctttagtgaa
     1441 gctaagccgt tgatgtttac ttgttccagc tattattttc cttttctttc gtcagaaatt
     1501 gataattttt ttcttccttt tgcacgacgt gcgttcaata cagcacgacc acctttagtc
     1561 gccatacgct cacggaaacc gtgtttgttc tttctttttc ttaccgatgg ttgaaacgtt
     1621 cttttcatct tatttcaatt ctaaaatttt tataattgtt gatttccagc attattctgc
     1681 taaaattcgg actgcaaaga tacaaaatag tattaaaaag tctatttttt tatgtgctat
     1741 tttgctaatt agttaattat ctaattgtta tcggttcatt gtcagcaaga attcttcatt
     1801 gcttctagtg cctttcatct gttgcaataa gaatttcatg gactcttctg agttcatatc
     1861 tgctaaatgg ttgcgcaaaa tccacatttt ttgtaatgca tctttgtcta acaacaaatc
     1921 atctcttcgt gtagaggaag ctaccacgtc aacagctggg aaaattctac gattcgacaa
     1981 ttttctatcc aactgcaatt ccatattacc agtgccctta aattcttcaa aaattacttc
     2041 gtccattttt gaacctgtat ctgttaatgc tgttgctata atggttagcg agccgccgtt
     2101 ttcgatgtta cgcgctgcac caaagaactg tttcggcttg tgtaaggcat ttgcctccac
     2161 accaccagac agcaccttgc ccgacgacgg tgcgactgta ttgtgtgcac gtgctaagcg
     2221 tgtaatggaa tctaataaga ttactacatc atgaccgcac tctaccagtc gttttgcttt
     2281 ttgcaacacg atactcgcta ctttcacatg tctatctgct ggctcatcga aggtagatgc
     2341 gacaacttcg gctttcacgc tgcgttgcat atctgttacc tcttcagggc gttcatctat
     2401 taataagata attaaatata cttctggatg gttcttggca atggaattgg caatgtcttt
     2461 cagcaacatc gttttacctg tttttggctg cgctactatc ataccacgtt gtccttttcc
     2521 tataggtgta aataaatcaa caatacgtgt agagtaatta ttggcctctt gaaataagtc
     2581 taatttttct gtaggaaaca atggtgtcaa ataatcgaat ggcactctat cacgtatttc
     2641 ttgtggcgtt ttgccattga tagattctac acgcaaaagt gcaaaatatt tctcaccttc
     2701 ttttggtgga cgaatgttac cgacaatagt atctccagtt tttagaccta tcaattttat
     2761 ttgtgatggc gacacataca catcatcagg agatgacaaa taattataat ctgaggaacg
     2821 caaaaaacca tagccttctg gtataatttc caacacacct tcgcctagaa taacaccatc
     2881 taaatctata ttaaaatcta tctttttttc ttcaggaagc gccacttcat cattttgctc
     2941 aatagcatgt atgatgtctg ccacactttc tcttgatggt tcaacatgtg tttcttcttg
     3001 tattttttct tctacttctg ctgctacttc ttcttggata actgattgtt gaatttctgc
     3061 ttttttgtgt ggcgctgatt gtttattctc tgcatctaca ttttcttttg ttttttttgc
     3121 aggcactacc ttttctgcca ctttacgcgc aatgcgtgtg cgttcctttt tttccttttt
     3181 aggtgcacca tcgttcgcgt ctgtctctaa atttttcaca gattcatctt ctttaatttc
     3241 ttgctcttgc atatattgtt ttttgagaat aagtttgttg aatgtgggtt tattggtgat
     3301 tggctttgat ttgatatcta tttcatcttc cgtaatcttg ttctaattgc atattgcatt
     3361 tcgataaaaa tgacatcacc atataaaaat tatacttaat tgaagtaaaa tgtaaaagca
     3421 ttaaaattac ggattctgaa ttcgatttga gattgttcaa taaaacgcga ggaaatattg
     3481 attgatatcg caaagtaaca caaattttat gaaaacgatg ctttttatcg gacttttttt
     3541 aaaaaaatac caaattatag ggtgaagtat taaatacaag atgcttaaaa actatctttg
     3601 caagacaata aacaataatg cttactttag aacaattacg aaaagacacc acctttgcca
     3661 aagaacgctt agcacataaa cacataaaag atgttcattt tgtcgatgaa ataattgcat
     3721 ttgacgacca acgaaaaaca atcaaagcgc aagttgacga cttgttgtca caacgcaata
     3781 cattgtcgaa agaaattggt gcgctgatga aagaaggca
//
LOCUS       DAOQQV010000169         3792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_11732_length_3792_cov_8.682901, whole genome shotgun sequence.
ACCESSION   DAOQQV010000169 DAOQQV010000000
VERSION     DAOQQV010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11732_length_3792_cov_8.682901"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLL59_11950"
     CDS             <1..219
                     /locus_tag="PLL59_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HMY24568.1"
                     /translation="MSTKIIDVSGSEAKEYRGVVPARSVVIPGTIPKEFPAGTYQVPC
                     ALIIGKRKESTDTKTSLNDALRDFDLSV"
     gene            294..1400
                     /locus_tag="PLL59_11955"
     CDS             294..1400
                     /locus_tag="PLL59_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMY24569.1"
                     /translation="MTIGFDGKRLYDNKTGLGNYSRTLLHRLIEFYPNETYRIFVHHK
                     YFDDSPYKYQHFVDNTVVSDAFSADLWRFKNVEEDISKQGISIYHGLSNEVPDLPPTI
                     KKVVTIHDVIFHKLPKTFPFIDRQMYAYKTKKACHVADAIIAVSEQTKQDLIELTKTP
                     EEKIHVVYPTWNREYEHECNYVLKEEYFARYGLPRDFVLYVGAISKRKNFLRLLQAMM
                     LPENQDKHLVAVSNGGDEYGAAEEFIYDKELEGRVFFLKDLPWYELPIIYHMSQGLVY
                     PSMYEGFGLPILEALRCGKPVITSNLSSMPEVGGDACIFIDPTNVEEISAAINFIYYN
                     IDLAKEIKQKALHQIQKFNPEKMTEKMMNVYKSL"
     gene            1412..1978
                     /locus_tag="PLL59_11960"
     CDS             1412..1978
                     /locus_tag="PLL59_11960"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMY24570.1"
                     /translation="MKNDTTYIFQLAEQLQQGKTILYPTDTIYGIGCDATNEAAVDKI
                     FAIKNRPKEKSLIVLVDSVAMLQKYVAVNDQIIELINSFELPTTLIYQNPKGIAKNAI
                     NHDNTIAIRIVKHQFCQDVIQQLNKPIISTSANISGEKNPVYFDEISLDIKNKIDVLV
                     PAQYDTSVYKFPSKLIKIKTDNTIEYLR"
     gene            2038..3453
                     /locus_tag="PLL59_11965"
     CDS             2038..3453
                     /locus_tag="PLL59_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMY24571.1"
                     /translation="MQLQFTPRENELLQIVASIADELQVEAYVVGGFVRDKILQRNCK
                     DIDIVCVGSGIELAKKVAESMSPKPHVSYFKNFGTAQIKLNDIELEFVGARKESYRRE
                     SRKPIVEDGTLEDDQNRRDFTINALAAALSAKNYGQVIDPFSGLEDLKNGIIRTPLNP
                     AITFDDDPLRMMRAIRFASQLNFKIEEKTYLAIEPLAERLKIISQERISDELNKIIMS
                     KMPSIGFKLLFNTKLLHQFFPEMVKLHGVAQQDNYGHKDNFYHTLKVLDNIAQNTDDL
                     WLRWSAIMHDIAKPATQRFEQGHGWTFHGHEVVGARWTQKIFSRLKLPLDEKMKFVQK
                     MVALHLRPISLSKDNITDSAIRRILYDAGDDIESLMLLCDADITSKNEVKVKRYKENL
                     KLVLQKIKEVEERDHIRNWQPPISGEIIMQTFNIKPSREVGIIKDAIKDAILDGEIQN
                     DFDAAFQLMLQKGKALNLTIS"
     gene            3469..>3792
                     /locus_tag="PLL59_11970"
     CDS             3469..>3792
                     /locus_tag="PLL59_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HMY24572.1"
                     /translation="MMLSSPNANKKFLIIIAGPTAVGKTATAIQIAKHFNTEIISSDS
                     RQIFKELSIGVARPSDQELAEVKHHFIANKSIHDYFSAGEYEREVLILLERLFLSNDV
                     VVLCGG"
BASE COUNT         1341 a          625 c          711 g         1115 t
ORIGIN      
        1 atgtccacaa aaataatcga tgtgtctggt agcgaagcaa aagaatatag aggtgtggtg
       61 cccgcaagaa gcgtggtcat tccaggaaca attccaaaag aatttccggc aggaacctat
      121 caggtgcctt gcgccttgat tattggcaaa cgtaaagaaa gcacagatac aaaaacatct
      181 ttgaacgatg ctttgagaga ttttgatttg tcagtataaa aaataatatg taaggctgaa
      241 cgtattgcag ccttcttcac cgctaacaaa taagcattaa caaataattg gatatgacaa
      301 ttggatttga cggaaaacgt ttatatgata ataaaacagg tcttggaaat tattccagaa
      361 cattgttaca tcgtttaata gagttttatc cgaatgagac ctacagaata tttgtgcatc
      421 ataaatattt tgatgacagt ccatataaat atcagcattt tgttgacaat acagttgttt
      481 ccgatgcatt tagtgctgat ttatggcggt ttaaaaatgt agaagaggat atttcaaaac
      541 aagggatttc catctatcat ggcttgagca atgaagtgcc cgatttgccg cctacaatca
      601 aaaaagtagt gaccattcac gatgtaattt ttcacaaatt accaaaaact tttcctttta
      661 ttgataggca aatgtatgct tataaaacaa aaaaagcctg tcatgtggct gatgcaatta
      721 ttgcagtgag cgaacaaacc aagcaagatt taattgagct aacaaaaacg cctgaagaaa
      781 aaatacatgt tgtctatcct acttggaata gagaatacga acacgaatgt aattatgtat
      841 tgaaagaaga atattttgca cgttatggct tacctcgcga ttttgtattg tatgtaggtg
      901 ccatcagtaa gcgcaaaaac ttcttacgtt tattgcaagc aatgatgttg ccagaaaatc
      961 aagataagca cttggtggct gtttcaaatg gtggtgatga gtatggtgca gcagaagaat
     1021 ttatttatga caaagagtta gaaggtcgtg tattttttct aaaagatttg ccatggtacg
     1081 agctaccaat tatctatcac atgtcgcaag gtttggtgta cccaagtatg tacgaaggtt
     1141 ttggactgcc aatactagaa gcattgcgtt gcggtaagcc tgttataact agcaatctgt
     1201 catcgatgcc cgaagtaggt ggcgatgctt gcatctttat cgatcctaca aatgtggaag
     1261 aaatatctgc tgccatcaat tttatttatt ataatattga tttagcaaaa gaaataaaac
     1321 aaaaagcatt gcaccaaatc caaaaattta atccagaaaa aatgacggaa aaaatgatga
     1381 acgtgtataa aagcttataa tcgatttgtt tgtgaaaaat gacactacat atatctttca
     1441 acttgctgag caattgcaac aagggaaaac aattttatat ccaacagata ctatttacgg
     1501 tataggttgc gatgccacaa atgaagcggc tgtcgataaa atatttgcta tcaaaaacag
     1561 acccaaagaa aagagcttaa ttgtgttggt agattcggta gccatgttgc aaaaatacgt
     1621 tgctgtgaat gatcaaatta ttgaacttat caactcattt gagttgccca caacgctgat
     1681 ttatcaaaat ccaaaaggca tagcaaaaaa tgcaattaat cacgacaata cgattgcaat
     1741 tcgaatcgta aagcatcagt tttgtcaaga tgtaatacag caactcaaca agccaattat
     1801 ttctacatca gcaaatattt ctggtgaaaa aaatcctgtt tattttgatg aaatttcatt
     1861 agatatcaaa aataaaatcg atgttttagt tccagcacaa tacgatacat cagtgtataa
     1921 atttccatca aaattgataa aaataaaaac agataacaca attgaatatt tgcgctaagt
     1981 aattttctta ctacttatta cttactactt attacttatg cgtattttcg tagtcacatg
     2041 cagctccaat ttacaccaag agaaaatgaa cttctacaaa ttgtagcaag tattgctgat
     2101 gaacttcagg tagaggcata tgttgtaggt ggttttgtgc gcgataaaat attacaaaga
     2161 aattgtaaag acatagacat tgtatgcgtg ggcagcggca tcgagttggc aaaaaaagtg
     2221 gcagaaagta tgtcgcccaa accacatgtt tcttatttta aaaattttgg aacggcgcaa
     2281 ataaagttaa atgatattga actagaattt gtaggcgctc gcaaagaatc ctaccgtaga
     2341 gaatcgcgta agcctattgt agaagatggt actttggaag atgaccaaaa tcgaagagat
     2401 ttcaccatca atgcattagc tgctgcattg tctgccaaaa attatggaca agttatcgat
     2461 ccattttctg gtttagaaga tttgaaaaac ggtattattc gcacaccttt aaatccagca
     2521 attactttcg atgatgatcc attgcgtatg atgcgtgcca tacgttttgc ttcgcaattg
     2581 aattttaaaa ttgaagaaaa aacatatctt gcaatcgagc cattagcaga acggcttaaa
     2641 attatttcgc aagaaagaat aagtgatgaa ctaaataaaa tcatcatgtc gaaaatgcca
     2701 tcgattggtt ttaagttatt gttcaataca aaattattgc atcagttttt tcctgaaatg
     2761 gtgaaactac atggtgtggc gcagcaagat aattatggac acaaagataa tttttatcac
     2821 accttaaaag tgttggataa tatagcacaa aatacagatg atttatggtt acgatggtca
     2881 gctatcatgc acgatattgc caagcctgcc acacaacgat ttgaacaagg acatggttgg
     2941 acattccacg gccatgaagt tgttggcgca cgatggacac agaaaatttt ctcgcgttta
     3001 aaattgccat tagacgaaaa aatgaaattt gtacaaaaaa tggttgcctt gcatttgcgt
     3061 ccaatttcat tgtctaaaga caatatcaca gatagtgcca ttcgcaggat tttatatgat
     3121 gcaggtgatg atatagaaag tttaatgctt ttatgcgatg cagatattac ctcaaaaaat
     3181 gaggtaaaag taaaacgcta caaagaaaat ctaaagctgg tattgcagaa aataaaagaa
     3241 gtagaagagc gcgaccatat tcgcaattgg caacctccta tttctggcga aattatcatg
     3301 caaacattta acatcaaacc atctcgcgaa gtaggcatta ttaaagatgc aatcaaagat
     3361 gcaattttag atggcgaaat acaaaatgat tttgatgccg ctttccagtt gatgttgcaa
     3421 aaaggcaaag cgttaaattt gactattagc taatttgaaa accaaacaat gatgctatcg
     3481 tcacccaacg caaacaaaaa atttcttatc atcattgctg gtcctactgc agttggtaaa
     3541 acagcaactg caatacaaat tgctaagcat ttcaacacag aaattatctc ttccgattct
     3601 aggcagattt tcaaagaatt atccattggc gttgcacggc caagtgatca ggaattggca
     3661 gaagtaaaac atcattttat tgccaataaa agcatacacg attatttttc tgctggcgaa
     3721 tatgaacgcg aagtgcttat tttattagaa agattatttt tgtcaaatga tgtggtagtg
     3781 ctttgcggtg gc
//
LOCUS       DAOQQV010000170         3759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_11899_length_3759_cov_10.310475, whole genome shotgun
            sequence.
ACCESSION   DAOQQV010000170 DAOQQV010000000
VERSION     DAOQQV010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11899_length_3759_cov_10.310475"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3271)
                     /locus_tag="PLL59_11975"
     CDS             complement(<1..3271)
                     /locus_tag="PLL59_11975"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMY24573.1"
                     /translation="MRRFTQAKNILFFAVLSFVSFNSIAHDTETDEADLLNKYQQQLH
                     FIQNKGQWDDNDIYRAQTFASTVQIRKDGFLLSVIDQQKLIESYELENEMEEADAKHL
                     PRPNRSVVIPQHAWMVRFLGMNKAAKIESKFQDKTYYNYFIGNDKSKWASSVNSYEEV
                     WYKNVYTNIDARLYAAEDLSLEYDMIVRPGANVADIKLAYEGLHGMYINKEGQLCIKT
                     IFGESVYPKPIAYQLKGGKRVSVKCEYTVAKGEILGFEMGEYDKLAPLVIDPIALKWA
                     VYVAYQSTTGSGHNHGIEVDQDQDLYVVGRLSSTNFPTTAGVFQTTATGTNTHGFVAK
                     MSVPNGVNGAGNFIWATYVSGTTAGDNPYSIKLDAAKNSYITGVTASTNFPTTAGAYD
                     VTHNGGNDGFVTKLNSTGTALLYSTFIGSAGMDESNFLYVTPTNEVWIAGRTTNAAFP
                     ITAGAIDATFAGAGEAFLAKFNATGTSLLYSTFYGGAGDDAFVCLRPSGVNELIAIGT
                     TSSDNTGNAIATAGAFQTTRSGTALNGMLMKFNTSTNTRTWGTYINPTAGGDLTLTCG
                     QVDNGGNIYFGGHTPGVVAACITAGVYDASFNGGGRDFYLGKINAAGSSLLAGTYIGG
                     NAAETNLMGLNIDDFGKVYAFGYTNSSSPSLSTTSNPLQAANNGGQDAIFFKISSDYS
                     TMEYLSYWGGSNDETDPIGFDGIKFSECKAYTAMTTQSANAPMTRNGFQINKTSGTIQ
                     EPAVTVWSNPPDVDVDTITGNENVCYGTAPLLDINGSASTYITSDISRAGVVTPHPGL
                     GTLTYSWSKSTDSINFTVIPAATGQNLTVAEIGVLTQTTHFKRNIASDFCKGGVIVTK
                     KVVNIAIPDGTESNGGIVCRGATINLTATGETGATFNWTGPNSFSATGANQTISNAQL
                     VNQGDYYVTQTVAGCTSPPDTVAVSIINCPPVAINDAYSVNEDQVLSDDVTTNDSDPN
                     GDPLTITLDNGNTATANGTLVLNNDGTFTYTPNPNFNGVVSFTYSACDNGVPLPVLCD
                     TATVTITVNSVNDVPTAEADDTTVPEDGSVTVPVLSNDTFGGDGPSTGTISIVTPPTN
                     GT"
BASE COUNT         1143 a          727 c          638 g         1251 t
ORIGIN      
        1 ctgtaccatt tgtcggtggt gttacgatgc ttatcgtgcc tgtgcttggg ccatcgccgc
       61 caaatgtatc gttgcttaaa actggtaccg ttacactacc atcttctggt acggtcgtgt
      121 cgtctgcttc cgctgtcggt acatcgttca ctgaattaac agtaatcgtt acagtagcag
      181 tgtcacataa gacaggtaaa ggtacgccat tatcacaagc actatatgtg aaagaaacta
      241 cgccattaaa atttggattt ggtgtatatg taaatgtacc atcattattt aaaaccaaag
      301 taccgtttgc tgtagccgta ttgccattat ctaatgttat cgtaagtgga tctccattag
      361 gatcactgtc attagttgtt acatcatcac ttaaaacctg atcttcatta acactatatg
      421 catcattaat agctacaggt ggacaattaa taatagaaac tgccactgta tctggtgggg
      481 aagtacaacc tgcaactgtt tgcgttacat agtaatcacc ttggtttact aattgtgcat
      541 ttgaaatcgt ttgattagca ccagtagcag aaaaagaatt tgggccagtc caattgaaag
      601 ttgcacctgt ttcacctgtt gctgttaggt tgattgttgc accacggcac actataccac
      661 cgttagattc tgtaccatca ggaatagcta tattaactac ttttttagtg acaattacac
      721 cgcccttaca gaaatcagaa gcaatattac gtttaaaatg agtcgtttgt gttaatacac
      781 caatttcagc aacagtaaga ttttgaccag tcgctgcagg aattactgta aaatttatac
      841 tatcagtaga ttttgaccaa gaataagtaa gtgtacctaa accaggatgt ggagtaacaa
      901 caccagctct gctgatatct gaagtgatat aagtagaagc gctaccgttt atgtctaata
      961 atggcgcagt accataacaa acattttcat tgcctgtaat tgtgtccacg tcaacatctg
     1021 gagggtttga ccaaaccgta acagctggct cttggatcgt tccacttgtt ttattaattt
     1081 gaaatccatt acgtgtcatt ggcgcatttg ctgactgagt ggtcatcgca gtatatgctt
     1141 tacactcgct aaatttaata ccatcaaaac ctattgggtc tgtttcatcg tttgaaccac
     1201 cccaataaga aaggtattcc atggtagaat agtccgatga aatcttgaag aatattgcat
     1261 cttggccacc attgttagct gcctgtaatg gattagatgt tgtagataaa gaaggcgatg
     1321 atgagtttgt atatccaaaa gcatatactt taccaaaatc atcgatattt aaacccatta
     1381 gatttgtctc agctgcatta ccacctatat aagtccctgc taaaagagaa gaacctgctg
     1441 catttatttt acctaaataa aaatctcgac cgccaccatt aaaagatgca tcatatacac
     1501 ctgcggtgat acaagctgca accacccctg gcgtatgtcc accgaagtat atattgccac
     1561 cattatctac ctggccacat gtcaaggtaa gatctccgcc agccgttgga ttaatatatg
     1621 taccccatgt acgtgtattt gtactcgtat taaatttcat gagcatacca ttaagggctg
     1681 ttcctgagcg agtagtttga aaagctcctg ctgtagcaat agcatttcct gtattatcag
     1741 aagaagtagt accgatagcg attaattcat ttaccccgct tggtcttaaa catacaaaag
     1801 catcatcacc agcgccacca taaaaagtag aatataacag agatgtaccc gtggcattga
     1861 atttcgcgag gaatgcttct cctgcacctg caaatgtagc atcgattgct cctgctgtaa
     1921 taggaaatgc tgcattcgtt gttctaccag caatccaaac ttcatttgtt ggtgttacat
     1981 acaagaagtt tgactcatcc attccagcag aaccaataaa tgttgaatac aataaggccg
     2041 ttccagtact attcagttta gttacaaaac catcattacc gccattatgg gtaacatcat
     2101 atgcacctgc tgttgttgga aaatttgtag aagcagtaac acctgtgata taagaatttt
     2161 tagcagcatc taacttaatt gaatatggat tatcacctgc tgttgtacct gaaacatagg
     2221 ttgcccatat aaaattacct gcgccattta caccattggg cacactcatt ttagcaacaa
     2281 atccgtgtgt attggttcca gtagctgtag tttgaaaaac acctgctgtt gtaggaaaat
     2341 ttgttgaact taagcgacca acaacatata aatcttggtc ttgatctact tcaataccgt
     2401 ggttatggcc agaacctgtt gtgctttgat aagccacata aacagcccat tttaaggcaa
     2461 ttgggtcaat tactaatggt gctaatttgt catactcacc catttcgaaa cctaatattt
     2521 cgcccttcgc cactgtatat tcacatttta cacttactct ttttccgcct tttaattggt
     2581 aagcgattgg ttttggataa acactttctc caaatattgt tttaatacaa agttgacctt
     2641 ctttattaat atacattcca tgaaggcctt catatgctaa cttaatatca gcaacgtttg
     2701 cgccaggtcg aacaatcata tcgtactcta gagacaagtc ttcagctgca tataaacgag
     2761 cgtcaatatt tgtatataca tttttatacc aaacctcttc gtagctattc acagaagacg
     2821 cccatttgct tttatcatta ccgataaaat agttgtaata tgttttgtct tgaaattttg
     2881 attcgatttt ggctgcttta ttcataccta agaatctgac catccaagca tgttgtggaa
     2941 tcacgacaga acgatttggt cttggcaaat gttttgcatc tgcttcttcc atttcatttt
     3001 ccaactcata gctttcaatc aatttttgct gatcgattac agaaagtaga aaaccgtctt
     3061 tacgaatttg aacagtagaa gcaaaagtct gtgcgcgata aatatcatta tcgtcccatt
     3121 ggcctttatt ttgaataaag tgtaattgtt gctgatattt attcaacaag tcagcttcat
     3181 cagtttctgt gtcgtgtgct atactgttga agctcacgaa agacaatact gcaaaaaata
     3241 gtatattttt tgcttgtgta aatctcctca taattcttaa attatttatt atagtatttt
     3301 tagagtcttg catgttgggt cgtttgtttc taaaatttgt tttaacattt ttatatgtca
     3361 gtactaagca gtgtagataa tgtatttaaa aaaatggttg catttattta taaaaaaaca
     3421 tttctcacgt tttagtaaaa attaatccta tttttcaatg ttttttttga attttatgtc
     3481 attaatcata aaaaagcaaa aaaataccta agttaacaat tacacaaaag taggaaataa
     3541 atttacattt aattgcattt ttgttagttt tttttagttt tttttagttt ttgttataat
     3601 taattaaaat acaaaacatt acacattaga ttttatctgc aatttgaaat tttaaataag
     3661 aaaaaaggtt accctttcgg ataaccttac tgccagttgg atttatctat tcattttaga
     3721 acggacaaac tttacaaggg tctacttgca tgtcgaagc
//
LOCUS       DAOQQV010000171         3741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_11981_length_3741_cov_9.424308, whole genome shotgun sequence.
ACCESSION   DAOQQV010000171 DAOQQV010000000
VERSION     DAOQQV010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11981_length_3741_cov_9.424308"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..921)
                     /locus_tag="PLL59_11980"
     CDS             complement(58..921)
                     /locus_tag="PLL59_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMY24574.1"
                     /translation="MSNNFAIIGVAGYIAPRHLKAIKEVNGNLMAAYDIADAVGILDT
                     YFPACDFFTNETSFFEFLAQQQTLDYLVICSPNYLHEAHCLAGLQLATTIICEKPLSL
                     TVDSLHTLKAAQQQSGKQIFTILQLRLLPMLIQLKKEMEQSTTNKFEVNIEYYTPRGK
                     WYHQSWKGDIEKSGGIATNIGIHLFDLVTWLFGNCKQLHVEKNTKTTSIGTLHLDKAS
                     IHWNLSIDSNLRAKRQMSINGKVYEFTEGFSDLHTQAYREIIKGNGFTIDNIFPSVAI
                     VEQIRKSIPSA"
     gene            complement(960..1532)
                     /locus_tag="PLL59_11985"
     CDS             complement(960..1532)
                     /locus_tag="PLL59_11985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMY24575.1"
                     /translation="MNNIFKHETTIVDDGAIIGNNSKIWHFSHIMRAQIGENCSIGQN
                     VFIANNVSIGNGVKIQNNVSIYEGVVLEDDVFVGPSVVFTNVINPRSFIERKTEYKTT
                     LIKKGATIGANATIVCGNTIGEYAFIGAGCVITKTVKPYALMKGNPAKQDGWMSEAGA
                     KLVFENNIAICAIRHEKYKMENNFCKKVID"
     gene            complement(1525..2616)
                     /locus_tag="PLL59_11990"
     CDS             complement(1525..2616)
                     /locus_tag="PLL59_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HMY24576.1"
                     /translation="MDVPMIDLKAQYLSIKDEIDEAIQHTLYRADFIQGNAVHELEQN
                     LAAYTGMQHVIGCANGTDALQLAIMALQLPKASKIIVPAFTYIAPVEVITFLGYEVVF
                     CDVNTQDFNTSLSHIKAVFCEDVKAIIVVHLFGQPCVDIENIDAFCKSNNIALIEDNA
                     QSLGAIKNIKRNSIVTTSFFPTKNLGAYGDAGAVFCNDEALARKIRMLASHGQSKKYI
                     HDAVGVNSRLDTLQAAILNVKLKYIDTYNHKRKVNAHYYQQQLSNIKAIELPLITADH
                     IFHQYTIIIKNGKRDELQIFLAQKGIVSTINYPIPAYQQQAYLQDIELKNTGILCASV
                     LSIPVFPELSDTQLSYICEMIKLFFATHE"
     gene            2661..3737
                     /locus_tag="PLL59_11995"
     CDS             2661..3737
                     /locus_tag="PLL59_11995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMY24577.1"
                     /translation="MKKQRILFLSPYPFGKAPSQRLKFEQYYSHFENNGFELSTSSFI
                     DDEFWKVIYQPGFLFGKILGTLRGYVRRVIDLCRLRQYDIVYIHLWVTPLGPPFFEWL
                     ISKIAKKVVYDIDDMIYLNQPKTRFIDKIKGRNKPLALMKYADAVIVCSPFLKQFVQQ
                     YNTNVHTILATYDTDKSTPKIHSPKNTITIGWTGTHSTLKYLKLVEDTLIQLSQNRKI
                     EFLVICNAPYHHERIPVRNINWTAAQEIEDLKQIDIGIYPLEKEEWVLGKSGNKALAY
                     MHVGVPCVATDFGTNSLVIQNGEDGFLVDNTTMAWYNSLLYLVDSTDERIRIGKNARL
                     AVEQHFSIKANQHIYLSVLKNLAE"
BASE COUNT         1177 a          637 c          655 g         1272 t
ORIGIN      
        1 ttggagattc ccactttcgt gggaatgaca ttcgtataaa tacaatttgc ttattgctta
       61 tgcacttggt atggattttc tgatttgttc gactatagca acagacggaa atatgttatc
      121 aatagtaaag ccattacctt taatgatttc acgataggct tgtgtatgta aatcggagaa
      181 gccttcggtg aactcataca cttttccgtt aatggacatc tgtcgtttgg ctcttaaatt
      241 gctgtcaata ctaaggttcc aatgtatgct agctttatca agatgcaatg tgccaatgct
      301 tgtcgttttt gtattttttt cgacatgtag ttgtttgcaa ttaccaaata accatgtgac
      361 taaatcaaat aaatggatac caatgtttgt agcaattccg cccgattttt cgatatcacc
      421 tttccagctt tgatggtacc attttccgcg tggtgtgtaa tattcgatat tcacttcaaa
      481 tttatttgtg gtggattgtt ccatttcttt tttcaattgt attagcattg gcagtaatcg
      541 caattgaagt atagtgaata tttgtttgcc actttgttgc tgtgctgctt tcaatgtatg
      601 caaactatct actgtaagtg ataaaggttt ttcacaaata attgttgttg ctagttgtaa
      661 acctgctaag caatgtgctt catgtaaata attgggagag caaataacaa gatagtctag
      721 tgtttgttgc tgcgcaagaa attcaaagaa tgatgtttca tttgtaaaga aatcacaagc
      781 cggaaaataa gtatctaaaa ttcctacagc atctgctata tcataagcag ccattaaatt
      841 tccatttact tctttaattg cttttagatg gcgtggtgca atgtagccag caacaccaat
      901 gatggcaaaa ttgttagaca tgtttatttg cttgtcgatt caatgtataa atacgaaagt
      961 tagtcaatta cttttttaca aaagttgttt tccattttat atttttcatg acggatagca
     1021 caaattgcaa tattgttttc aaatactaat tttgctcctg cttcactcat ccagccgtct
     1081 tgctttgccg gattgccttt cataagcgca tacggtttta ctgttttggt aataacacag
     1141 ccagcaccaa taaatgcata ttcgccaatc gtatttccac aaacaattgt ggcattagcg
     1201 ccaatcgttg cacctttttt aatcaatgta gttttatatt ctgtctttct ttcaataaaa
     1261 cttctcggat tgatgacgtt tgtaaaaacg acggaagggc caacaaatac atcatcttcg
     1321 agaacaacac cttcatatat agaaacatta ttttgaattt tcacaccatt gccaatgctt
     1381 acattgttgg caataaatac attttgtcca atgctacaat tttcgccaat ttgcgcacgc
     1441 ataatatgac tgaaatgcca tattttacta ttgtttccaa taatggcgcc atcatcgaca
     1501 atggtagttt cgtgtttaaa aatattattc atgcgttgca aaaaataatt ttatcatttc
     1561 acaaatatac gacaattgcg tgtcgctcaa ttctggaaaa acaggaattg ataaaacaga
     1621 tgcgcataaa atgcctgtat ttttgagttc aatatcttga agatatgcct gttgttgata
     1681 ggcaggaatg ggataattga tagtagaaac aattcctttt tgtgcaagaa aaatttgtag
     1741 ttcatcgcgt tttccatttt ttataataat ggtatattga tgaaaaatat gatctgctgt
     1801 aattagtggt aattcaatag ctttaatatt cgacagttgt tgctggtaat aatgcgcatt
     1861 tacttttctt ttgtgattat aggtgtcaat atatttcagc tttacgttga gtatagcggc
     1921 ttgcaaagtg tctaagcgag aattaactcc gacagcatcg tgaatatact ttttagattg
     1981 tccgtgtgac gcgagcattc taatttttct tgctaatgcc tcatcattac agaatacagc
     2041 accagcatcg ccataagcac ctaaattttt tgttggaaaa aaagacgttg taacaatgct
     2101 atttcgtttt atgtttttta ttgcacctaa agattgcgca ttgtcttcaa ttaatgcaat
     2161 attatttgac ttgcaaaaag catcaatgtt ttcgatatct acacatggtt gaccaaataa
     2221 atgaacaacg ataatcgctt ttacgtcttc gcaaaataca gcttttatat gcgataatga
     2281 tgtattaaaa tcctgtgtgt tcacatcgca aaacactact tcatagccaa gaaatgtaat
     2341 aacctctact ggtgcaatat aagtaaatgc tggtactata attttgctag cttttggtag
     2401 ctgtaaagcc ataatggcta attgcaaggc atcagttcca ttcgcacagc caattacatg
     2461 ttgcatacca gtgtatgcag ctaagttttg ttccagctcg tgcacagcat tgccttgaat
     2521 aaaatcagcg cggtaaagcg tgtgctgaat ggcctcatca atttcatctt tgatagataa
     2581 atactgtgct ttcaaatcta tcattggtac atccatactc aataatacaa aaaattagtt
     2641 tattttagga gtcgcattat atgaagaagc aaagaatctt atttctttca ccgtatccat
     2701 ttggtaaagc gccgagccaa cgtttaaaat ttgaacaata ctattctcat tttgaaaata
     2761 atggtttcga attaagcaca agctcattta ttgatgatga attttggaaa gtaatttatc
     2821 aacccggttt tttgtttggg aaaatcttgg gcacgcttcg aggctatgtg cgcagagtca
     2881 ttgatttatg taggcttcgc caatatgata ttgtgtatat acatctttgg gtgacgccat
     2941 taggtccacc tttttttgaa tggcttattt caaaaatcgc taaaaaagta gtgtatgata
     3001 ttgatgatat gatatatctt aatcaaccca aaacacgctt tatagataaa ataaaaggaa
     3061 gaaataaacc cttagcatta atgaaatatg cagatgctgt aattgtatgt tcgccatttc
     3121 taaaacaatt tgtacaacag tacaatacga atgtacacac cattttagct acatacgaca
     3181 cggataaatc gacaccaaaa attcattcac caaaaaatac aattacaatt ggctggacag
     3241 gcacacattc tactttaaaa tatttaaagc ttgtagaaga tacattgata caacttagcc
     3301 aaaatagaaa aattgagttt cttgttattt gcaatgcgcc ctaccatcac gagcgcatac
     3361 ctgttcgcaa tatcaactgg acagctgcgc aggaaattga agatttaaaa caaatagata
     3421 ttggtattta tccattagag aaagaagaat gggttttagg caaaagtggc aataaggcat
     3481 tagcttatat gcatgttggt gtgccttgtg tggccacaga ttttggcact aattcgctag
     3541 taatacaaaa cggagaagat ggttttttgg tggataatac tacaatggcg tggtacaata
     3601 gtctattgta tttagtagat tcaactgacg aaagaatacg cattggaaaa aacgcacgat
     3661 tggccgtaga acaacatttt tctattaaag cgaatcagca tatttatctg agtgtgctga
     3721 aaaatttagc agaataataa g
//
LOCUS       DAOQQV010000172         3561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_12910_length_3561_cov_6.781232, whole genome shotgun sequence.
ACCESSION   DAOQQV010000172 DAOQQV010000000
VERSION     DAOQQV010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12910_length_3561_cov_6.781232"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLL59_12000"
     CDS             complement(<1..526)
                     /locus_tag="PLL59_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HMY24578.1"
                     /translation="MKNWWKYLSILLLFYTVYFGFKGTIPELDILEQSIRNLYFHVPM
                     WFAMMLLMFVSMCYSILYLSKQQIDADIKAAAFAKVGFFFGFLGIVTGAVWARATWGA
                     WWVFQEVKLNGAAAALLIYAAYFILRGSFDDEEKKARFSAVYSIFAFTMFMVLINVIP
                     RISNSSLHPGNGGNP"
     gene            complement(599..1249)
                     /locus_tag="PLL59_12005"
     CDS             complement(599..1249)
                     /locus_tag="PLL59_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HMY24579.1"
                     /translation="MVNILKLVRKELQLEWRQKTALGTIFIYVLGTSFLIYLVFQGHI
                     TIPVWIALFWVIALFTTVNAVSRSFLKDANEQFYYLRSIATPLEIIFSKIIYNAFLIT
                     ILSLIIYLVMILFFNENIDNKGLFLLTIILGAIGFSNLFTLLSAITARTQNFVLLAIL
                     GIPIILPLLLLIVKMSELSNFAIQQKDIYMHLGAIAMLDVITLILSIVLFPYIWRD"
     gene            1311..1916
                     /locus_tag="PLL59_12010"
     CDS             1311..1916
                     /locus_tag="PLL59_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HMY24580.1"
                     /translation="MLVLNNIKITTSIFVIACMLFIQPAFAKKKKKDKAKTDYNFTSK
                     NRDSVWRKVVPFDLSNATFTALKSSNIDGILWFAKQQLGTPYKYASADPKNGGLDCSG
                     FLFYVYTHFKIKVPRTSKDYMNFGKSISTNKIQKGDVLIFTGSDASQKIAGHVGMVID
                     TNDGEISFIHGASGKGKGVTISKLSESYYTTRFLKAVRIVN"
     gene            2018..3496
                     /gene="accC"
                     /locus_tag="PLL59_12015"
     CDS             2018..3496
                     /gene="accC"
                     /locus_tag="PLL59_12015"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMY24581.1"
                     /translation="MKKILIANRGEIALRVMRTCKQMGISTVAIYSEADRNSPHVRFA
                     DEAVCVGAATSKESYLKIDTIIEVCKSLQVDGVHPGYGFLSENAVFAKACKDNGITFI
                     GPSVEAIEIMGSKLAAKQAVSKFDVPLVPGTDQPISDINEAKNLAEAIGFPILIKASA
                     GGGGKGMRIVNNVEEFEEQMKLAVSEATSAFGDGSVFIEKFVTSPRHIEIQILGDTHG
                     NIVHLFERDCSVQRRHQKVVEEAPSAVLTPALRKQMGEAAVQVAKSVNYIGAGTVEFI
                     VNANLDFYFLEMNTRLQVEHPVTEMITGIDLVKEQINIARGEKISFGQDDLKIQGHAI
                     ELRVYAEDPENNFLPDIGKLEAYHIPKGEGIRVDDGFDEGMDIPIYYDPMIAKLIAHG
                     SNRKDAIEKLKKAIIDYNIVGVKTTLPFGEFVLHHSAFTTGNFDTKFVDNYYHPSKIN
                     ETELALATLIAAKLFEEKGQTFEHVSEGNIKNETNWKNNRME"
BASE COUNT         1317 a          557 c          644 g         1043 t
ORIGIN      
        1 ctggatttcc accatttcca ggatgcaatg aggaattgct gatacgagga attacgttga
       61 ttaacaccat aaacatagta aatgcaaaaa tggaataaac agcagagaat cgcgctttct
      121 tctcttcgtc atcaaatgag ccacgcaaaa tgaagtaggc agcataaatg agtaatgctg
      181 ctgctgcgcc atttaatttt acttcttgaa aaacccacca agcgccccat gttgcacgcg
      241 cccaaacagc acctgtgaca atgcctaaga agccgaagaa aaaaccaact ttagcgaatg
      301 ctgcagcttt gatatctgca tcgatttgtt gcttgcttaa atacaatata ctataacaca
      361 tcgaaacaaa cattaataac atcatagcaa accacatggg cacatgaaag tataagttgc
      421 gtatagattg ttccaatata tccaattcag gtattgtgcc tttaaatcca aaataaactg
      481 tataaaacaa taacagtatg cttagatatt tccaccaatt tttcataatt atttttccaa
      541 atggaatttt taattgtcaa ttattaatga atagagtgct ttaaaaactg aaagcaactt
      601 aatcacgcca aatatacgga aacaaaacga ttgaaagtat gagtgttatc acatctaaca
      661 tggcgatagc accaagatgc atataaatat ctttttgttg aatggcaaaa ttggatagct
      721 cactcatttt tacgatgagt aataacaagg gcaatattat tggaattcct aagatagcaa
      781 gcaacacaaa attttgagta cgtgctgtaa tagcagataa taaagtaaat aaatttgaaa
      841 atccgatagc accaaggata attgtcagta agaaaagtcc tttgttgtca atattttcat
      901 taaaaaacaa gatcattact aaataaataa taagagataa aatggtaatt aaaaaagcgt
      961 tgtaaataat tttagaaaag atgatttcta atggtgttgc tattgagcgt aaataataaa
     1021 attgttcatt agcatctttt aaaaaggaac gcgaaacggc atttactgtt gtaaatagtg
     1081 caatcaccca aaataaagca atccaaacag gtattgtaat atggccttga aaaactaaat
     1141 aaatcagaaa gctagtgcct aacacgtaaa taaatatagt gcctagcgca gttttttggc
     1201 gccattcgag ttgcaattct ttgcgaacaa gttttagtat atttaccaca ctttgtattt
     1261 ttcaaaacaa aattagctta ttttggattg atttttgcta ataatagaat atgctagtat
     1321 taaacaatat aaaaattact acatctattt tcgtcattgc ttgtatgcta tttatacaac
     1381 cagcatttgc aaaaaagaaa aaaaaagata aagcaaaaac agattataac tttacaagca
     1441 aaaacaggga ttctgtttgg agaaaagtag ttccatttga tttatcaaat gccacattta
     1501 ctgcattaaa atcaagtaat attgacggca tattatggtt tgccaaacaa caattaggga
     1561 caccttataa atatgcttcc gctgacccta aaaatggtgg actcgattgt tctggttttt
     1621 tattttatgt atatacacac ttcaaaataa aagttccacg cacgtcaaaa gactatatga
     1681 attttggtaa aagtatatca accaataaga ttcaaaaggg tgatgtatta atctttacag
     1741 gctccgatgc tagccaaaaa atagctggtc acgtaggtat ggtcatcgat acaaacgatg
     1801 gtgaaatatc ctttattcat ggtgcttctg gcaaaggtaa aggtgtaact atttctaaat
     1861 tatcagaatc atattatact acaagatttt taaaggcagt acgaattgtc aattaaatga
     1921 aaaatcaaaa atagctaatg ctaattaatg gttgctaaaa gcgatgtgct ttttcacttt
     1981 catttaaaat acttattttt accctgcaag attaattatg aagaaaattt taatagcaaa
     2041 tagaggcgag atagctttac gagtgatgcg cacgtgcaaa caaatgggca tttcaacggt
     2101 tgcaatatat tccgaagcag atagaaattc gccgcatgta cgttttgctg atgaagccgt
     2161 ttgtgtaggt gctgccactt ctaaggaatc atatctaaaa atagatacta taatcgaagt
     2221 atgtaaatca ttacaagtcg atggtgtgca cccaggatat ggttttttaa gtgaaaatgc
     2281 tgtatttgca aaagcatgta aagacaatgg cataacattt ataggaccta gtgtagaagc
     2341 aatagaaata atgggcagca aattggctgc gaagcaagca gtatcgaagt ttgacgtgcc
     2401 attagtacct ggtactgacc aaccgatttc ggacatcaat gaagccaaaa accttgctga
     2461 agcaatcgga tttccgattt tgataaaagc aagtgctggt ggtggcggga aaggtatgcg
     2521 aattgtgaat aatgtggaag aatttgaaga acaaatgaaa ttggctgtat cggaagctac
     2581 ttctgcattt ggtgatggta gtgtttttat tgaaaagttt gttacttcac ctcgacatat
     2641 cgaaatacaa attttaggcg acacgcatgg aaatattgtc catctttttg agcgtgattg
     2701 ttctgtacaa agaagacatc aaaaagtagt agaagaggca ccgagtgctg tgctaacacc
     2761 agccttgcgc aagcaaatgg gcgaagctgc agtacaagtg gctaagtcag taaattacat
     2821 cggtgcaggc acagtagagt ttattgtaaa tgctaattta gatttctatt ttttagaaat
     2881 gaatacgcga ttacaagttg aacatcctgt tacagaaatg attactggta tagacttagt
     2941 gaaagaacaa atcaatatag ctcgtggcga aaaaatatct tttggacaag atgacttaaa
     3001 aatacaagga catgcgattg aattacgtgt gtatgctgaa gatcctgaga ataattttct
     3061 accagatatc ggaaaattag aagcatatca cataccgaaa ggcgaaggca ttcgtgttga
     3121 tgatggtttt gatgaaggta tggatattcc aatttattac gatcctatga ttgctaaact
     3181 aattgcacac ggtagcaaca gaaaagatgc catagaaaaa ttaaaaaaag caataattga
     3241 ttataacata gtcggtgtaa aaacaacctt accattcggt gaatttgtct tacatcactc
     3301 cgcttttaca acaggaaatt ttgacacaaa gtttgttgac aattattatc atcctagtaa
     3361 aataaatgaa actgaattgg cattagcgac attaattgca gcaaagctat ttgaggaaaa
     3421 aggccaaaca tttgaacatg tttcagaagg taacatcaaa aatgaaacaa attggaagaa
     3481 caatagaatg gaataaaata agagataaaa tataaaatgc gaagtaatac tacttagcat
     3541 ttttttttgc caaactactt a
//
LOCUS       DAOQQV010000173         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_13250_length_3504_cov_8.848362, whole genome shotgun sequence.
ACCESSION   DAOQQV010000173 DAOQQV010000000
VERSION     DAOQQV010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13250_length_3504_cov_8.848362"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLL59_12020"
     CDS             complement(<1..586)
                     /locus_tag="PLL59_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMY24582.1"
                     /translation="MSKNLLNTNMDTNTQQKGVTLLVIVAALGYFVDVFDLLLFGMVR
                     VSSLRSLGVAETQLEAIGLRLDNWQMLGMLLGGIFWGILGDKRGRLSVLFGSIVTYSI
                     ANSLNAYVHTIPQYEILRFIAGFGLAGELGAGVTLVNESMNKEKRGIATAFIAGFGVL
                     GAVFGCLIVLWLKDWRTCYLIGGIMGFALLILRIS"
     gene            complement(609..1655)
                     /locus_tag="PLL59_12025"
     CDS             complement(609..1655)
                     /locus_tag="PLL59_12025"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HMY24583.1"
                     /translation="MATTFNNFEFSVRPENHQVVKYKQIINQYLTKEEIKQLHIKSDW
                     KGLYEVASVWFWIIFAFALVGITTQLTSNKLILAVNIVIALFIIGGKQLGCAIIMHDA
                     SHYSLFKSKKLNDIIGNFFGAYPIFNNVEQYRPYHFQHHIATGTDDDPDINLVKGYPA
                     KIAGMMRKLFRDLIGLTGIKSELGLTAMHLGFIKYNLGNVIEKIPNENRPWKVIFRNA
                     YFNLRGPIFVNLMMFLILYAIGLPYLYLLWIGANLTTYNFCIRIRSIAEHSVVEDTHD
                     PYKNTRTTYANFIERLLFAPLHVNYHLEHHFLQNMPSYNSPAMHKMLKERGFYEHGLL
                     KQGYLQIIKMAIIK"
     gene            complement(1664..2338)
                     /locus_tag="PLL59_12030"
     CDS             complement(1664..2338)
                     /locus_tag="PLL59_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24584.1"
                     /translation="MNKRLFLLPGFGEDTFCFNNLIPYINKHKYKIIHVDYRPVLKKF
                     TFPFITVQQFSKQLVNTYAIQSEDKLLGHSMGGYFSFQTRELLGNEICMIGAFNDPQK
                     VIHTFPKMPRATMIAVLSGLIKTNMLKEYLLNKIKDENYRAVQEEIMDNFDTFSNYEL
                     ALMIEMNYQKKIDSILPNPLRIHDKNDKIIAAPDEEYTAIKGGHFCLNLYPEDTYLAM
                     QSFLEK"
     gene            2409..3458
                     /locus_tag="PLL59_12035"
     CDS             2409..3458
                     /locus_tag="PLL59_12035"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HMY24585.1"
                     /translation="MNKIRLPAEWEKQALIQIAFPSEQSDWIDYWHTVIPCYIQIIKT
                     ISSYQPLLVVCDNEEAVKHLLIDIDLTNIYFIQLPINDTWARDHGAITIESENGFQFY
                     DFMFNGWGLKFAADKDNLITNGLWENGIYNCDKLIIPDLVLEGGSIESDGNGVLMTST
                     NCLLSPNRNPHLSKQQIEEKLFSLFNLKKILWLEHGHLIGDDTDAHIDTLARFCDEKT
                     IVYVQCTDIDEVHYTSLKKMEDELKNLKTLDNEPYQLIPLPMADALFAPDDNRRLPAT
                     YANFLILNDVVLMPTYHVEQDDEALTQLQKAFPNKKVEGIDCAALLLQHGSLHCISMQ
                     YPEGSINFEVLKNKK"
BASE COUNT         1198 a          586 c          553 g         1167 t
ORIGIN      
        1 cactaattct caaaattaat aatgcaaaac ccatgatacc accaattaaa tagcaagttc
       61 tccagtcttt gagccataat acgattaagc aaccaaagac ggcacctaac acgccaaagc
      121 ctgcaataaa tgctgtggca ataccacgtt tttctttgtt catgctttca ttaacgagtg
      181 ttacgcctgc acctaattcg cctgctaaac caaagccagc aatgaatcgc aatatttcat
      241 attgtggaat cgtgtgcaca taagcattga gtgaatttgc gatggagtaa gtaactatcg
      301 aaccaaacaa tacagaaagc ctaccacgtt tgtcacctaa aattccccaa aaaataccac
      361 ctaataacat gcccaacatt tgccaattat ctaatcgcaa tccgattgct tcaagttgtg
      421 tttctgccac gccaaggctt cgcaaactgc ttacacgcac catgccgaag agcaataaat
      481 cgaaaacatc aacaaaatag ccaagagcag caacaataac caaaagtgtg acaccttttt
      541 gctgtgtatt ggtgtccatg tttgtgttta ataaattttt agacatgtat agattttctt
      601 gtagtgtatt atttaatgat agccatttta atgatttgta aatatccttg ttttagcaaa
      661 ccatgttcat aaaagcctcg ttctttcagc attttatgca tagctggtga attatatgat
      721 ggcatatttt gtaaaaagtg atgctctaaa tgatagttga catgcaaagg tgcgaacaat
      781 aagcgttcta taaaattggc atatgtcgtt cttgtgtttt tatacggatc atgtgtatct
      841 tctacaacac tgtgttctgc aatagaacga atgcgaatac agaaattata agtagttaga
      901 ttggcgccaa tccacaataa atacaagtat ggcagcccaa ttgcatataa aattaaaaac
      961 atcattaaat taacgaaaat tggtccacgt aaattaaaat aggcatttct gaatatcact
     1021 ttccaaggac gattttcgtt tggtattttt tcaatcacat taccaagatt atattttata
     1081 aatcctaaat gcatcgccgt caaacctagt tctgatttta ttcctgttaa gccaattaaa
     1141 tctctaaaca gtttgcgcat catgccagca atttttgcag gatagccttt taccaaatta
     1201 atgtcaggat catcatcagt tcccgtagca atatgatgtt gaaaatgata aggtctgtat
     1261 tgctctacat tattaaatat tggataagcg ccaaaaaaat tacctataat atcgtttaat
     1321 tttttgcttt tgaataagga ataatgtgat gcatcatgca taataatagc acagcctaat
     1381 tgcttgccac caatgataaa taatgcgatg acgatattga cagcaagtat taatttattg
     1441 cttgtaagtt gtgtagtaat acctactaat gcaaatgcaa atattatcca aaaccaaaca
     1501 gaggctactt cgtacaagcc tttccagtct gattttatat gcaattgttt tatctcttct
     1561 tttgtcaaat attgattaat aatttgctta tacttcacca cctgatgatt ttcagggcgc
     1621 acactaaatt caaaattatt aaaggttgtt gccataacga aaactatttt tctaaaaaag
     1681 attgcatagc aaggtaagtg tcttctggat ataaatttaa acaaaaatgg ccacctttta
     1741 ttgctgtata ttcttcatct ggcgctgcaa ttattttgtc atttttatcg tgtattcgca
     1801 atggattcgg taaaatagag tcaatctttt tttgataatt catttctatc attaatgcta
     1861 attcataatt actgaatgtg tcaaaattat ccatgatttc ctcttgtact gctctgtagt
     1921 tttcatcttt tattttattg agtaaatatt cttttagcat gtttgtttta attaagccag
     1981 acaaaacagc aatcatagtt gctctcggca tctttggaaa tgtatgaatt actttttgtg
     2041 gatcattaaa cgcaccaatc atacaaattt catttcctaa taattctctg gtttgaaatg
     2101 aaaaatagcc acccatagaa tgtcctaata atttatcttc tgattgtata gcgtatgtat
     2161 tgactaattg ttttgagaac tgttgaacag tgataaaggg aaaagtgaat ttttttaaaa
     2221 caggcctgta atcaacatgt atgattttat atttatgttt gtttatataa ggtattaaat
     2281 tgttgaaaca aaaggtgtct tcaccaaaac caggcaatag aaataagcgt ttattcatgt
     2341 aacgaaaata gagaatctaa ttgaaatttc atttctttaa gatataattt ataatgtatt
     2401 tttgcagtat gaataaaata cgcttaccag ccgaatggga aaaacaagct ttgatacaaa
     2461 tagcttttcc tagcgagcag tctgattgga ttgattattg gcatactgtc attccttgct
     2521 atatccaaat cattaaaaca atttcaagct atcagccttt attggtagtt tgtgataatg
     2581 aagaagccgt taaacacctt ttaatagaca tcgatttgac gaatatctat tttatacaat
     2641 tgccaatcaa tgatacatgg gcacgtgacc atggtgcaat aactattgaa tctgaaaatg
     2701 gttttcaatt ttatgatttt atgtttaatg gttggggtct taaatttgct gctgataaag
     2761 acaatttgat tactaatggc ttatgggaaa acggaattta taattgtgat aaattgatta
     2821 ttcctgattt agtgctcgaa ggaggaagta ttgaaagtga tggaaatggc gtactaatga
     2881 caagcacaaa ctgcctatta tcgcctaata gaaatcctca tttatcaaaa cagcaaattg
     2941 aagaaaagtt attctcactt tttaatttaa aaaagatatt atggcttgaa catggtcatt
     3001 taataggcga tgacacagat gcacatatcg atacacttgc ccgtttttgt gatgaaaaaa
     3061 caattgtcta tgtacaatgt acagatattg atgaggtgca ttatacttca ttgaaaaaaa
     3121 tggaagatga acttaaaaat ttaaaaacct tagataatga gccatatcaa ctcattccat
     3181 tgccaatggc tgatgcttta tttgctcctg atgacaatag aagattgccc gcaacatatg
     3241 ctaatttctt aatcctaaat gatgttgtac tcatgcctac ttatcatgtc gaacaagatg
     3301 atgaagcact tactcaactc caaaaagcat ttcccaataa gaaagttgaa ggaattgact
     3361 gtgctgcatt attactacaa catggttctt tacattgtat aagcatgcaa tatcctgaag
     3421 gaagcataaa ttttgaagta ttaaaaaata aaaaataaaa atgaaagtag gaattgttca
     3481 acaatcaaat accaatgata aagc
//
LOCUS       DAOQQV010000174         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_13478_length_3469_cov_6.327768, whole genome shotgun sequence.
ACCESSION   DAOQQV010000174 DAOQQV010000000
VERSION     DAOQQV010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13478_length_3469_cov_6.327768"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1102
                     /locus_tag="PLL59_12040"
     CDS             248..1102
                     /locus_tag="PLL59_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMY24586.1"
                     /translation="MSKIKGANVLVTGGANGIGKLMGLKSLQEGAANLVIWDINEDNL
                     QKTYREFTAKGYRNVYTFVVDVASVDDIERVATEVLLEIGNIDILYNNAGIVAGNKAF
                     WEYSARDIDKTVGINVNGVMHVTRVFLKDMIKQRRGHIINIASASSFISLPNGSVYAS
                     SKWAVFGWSESLRMELENEGADLHVTTVCPSYIDTGMFKGVKAPLLFPLLQPEDTANK
                     IIKAVKENTTVLMLPETLQIVPILKGIFPTNIFDKVAGFLGVYTSMKSFEGRAQEERV
                     PDKKSKVK"
     gene            1142..2524
                     /locus_tag="PLL59_12045"
     CDS             1142..2524
                     /locus_tag="PLL59_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HMY24587.1"
                     /translation="MATKTISAIKSTIDKQRDYFESNATRDVDFRIQQLKNFKKALQH
                     HESAIFEALQADFRKSNFETYATELGIIYDEVNCMLKHVKKWATPELVKDSIVNFPSR
                     NYIYHDPYGVCLIIGAWNYPLQLTLAPVVGAIAAGNTCIIKPPRAAIHTYHIIEKIIS
                     DTFNDNYIAVLDEHSDNAEMLAHRYDYIFFTGGVEIGRTIARAAAEFLTPTTLELGGK
                     SPCIVDKHADIEVAARRIAWGKYLNGGQTCVAPDYLLLHESVKEKFYKAFTKTVKEFY
                     GNNPQDSADYPRIINDRHFKRIAAMITDGNIIVGGKTDAETRYISPTLIEIDSLAHPL
                     MNDEIFGPVLPVLTINSIDDAIHIIKQYEKPLAFYLFSNNYSNQQKCLQTIQFGGGCI
                     NDTISHLANHELPFGGIGNSGSGAYHGKASFDTFSHKKSICHKVTWPDVPLRYPPYNG
                     KLMLIKQVMK"
     gene            complement(2539..>3469)
                     /locus_tag="PLL59_12050"
     CDS             complement(2539..>3469)
                     /locus_tag="PLL59_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24588.1"
                     /translation="YIDKIKNTAAAIRQKYPNLKIAVVAGVPTEEEDKTQRSNFMRQW
                     NIGLSKVNFYDGYIFHYYAACKPCDDDDFFENVYSKNLTALAPFQSNQLQDMQRIYKS
                     LYGENRKLWLTEWNVSNGEYLDNTFVQGAFVYEQFLEMIAINAKQNNYIDISSLHAID
                     GLITIFKGKQKPVFSKGNDNASVQYFAFKFLATTLSQNTNRCETSMTSEDKEIHKNLV
                     CQVFRNTQNNATYLHFVNRSAKEIELTFIKDKTSGLVQVNSIEASVPWATAGKTMYEK
                     KYREKVKPITIKTENITGNVITIAPYSFGYAEY"
BASE COUNT         1102 a          637 c          660 g         1070 t
ORIGIN      
        1 ctattcttat aatacaacgt aatcttccga acacttatgg tgaggacacg aaccatggct
       61 aaggtttgtt ggttgaaaac caacaaatgc gaggagtata aagttttatc tatcggtcgg
      121 tgtcctcgcc ggccgataga taaaaaatgg cacgaaatcg catgctatat aaacattcgt
      181 taattaaaaa cagactgtgc ctaatttacg atttttagca gtacatttaa tactctaaaa
      241 taagcttatg tcaaaaataa aaggcgcaaa tgtattagta actggtggtg ctaatggtat
      301 cggaaaatta atgggtttaa aaagtctgca agaaggtgct gccaacctcg tgatttggga
      361 catcaatgaa gataatcttc aaaaaaccta tcgcgaattt acagcaaaag gctatagaaa
      421 tgtttatacc ttcgttgtcg atgtcgccag tgttgatgac attgaacgag tagcgacaga
      481 agtattatta gaaattggca atatcgatat tctttataat aatgcaggta tcgtggcggg
      541 caataaagca ttttgggaat acagcgcccg agatatagac aaaactgtag gcatcaatgt
      601 taatggtgtg atgcatgtga ctcgcgtgtt tttgaaagac atgataaaac aacgaagagg
      661 ccacattata aatatcgcat cagcatcaag ttttatttcc ttgcctaatg gctctgtgta
      721 tgcatcgagt aaatgggctg tgtttggctg gagcgaaagc ctacgcatgg aattagaaaa
      781 tgaaggtgct gatttgcatg taacaaccgt ttgtccgagc tacatcgaca ctggcatgtt
      841 taaaggcgtg aaggcaccat tgctgtttcc attattacaa ccagaagaca ctgccaacaa
      901 aattataaaa gcagtgaaag aaaatacaac tgtattaatg ctgccagaaa ccttgcaaat
      961 tgttcctata ttaaaaggca ttttcccaac aaatatattc gacaaagtag ctggttttct
     1021 aggtgtttat acttcgatga aaagctttga aggtcgagcg caagaggaac gtgttcctga
     1081 taaaaaatca aaagtaaaat agtgtatttt tgcattccat cattttttaa tttcatcaac
     1141 aatggcaaca aaaaccattt ctgcaataaa atctaccata gacaaacaac gcgattattt
     1201 tgaaagcaat gcaacgcgtg atgttgactt tagaatacag caactcaaaa atttcaaaaa
     1261 ggcattacaa catcatgaat ctgccatttt tgaagcatta caagccgact ttcgaaaatc
     1321 aaattttgaa acctatgcca cagaattagg tatcatctat gatgaagtca attgcatgct
     1381 taagcatgtc aaaaaatggg caactcctga gttggtaaaa gatagtatcg tcaactttcc
     1441 atcgcgcaat tatatctatc acgatccata cggcgtatgt ttaattattg gcgcttggaa
     1501 ttatcctttg cagctcacct tagcacctgt tgttggcgct atcgctgccg gaaacacttg
     1561 tattatcaag cctccacgcg ctgctattca tacctatcat atcatcgaaa aaataatcag
     1621 cgatacattt aatgataatt atatcgccgt gttagatgaa cattctgaca acgcagaaat
     1681 gttggcacat cgttacgatt atattttctt tactggtggc gtagaaattg gcagaacgat
     1741 agcacgtgct gcagctgagt ttttaacgcc tacgacttta gaactcggcg ggaaaagtcc
     1801 atgtattgta gataagcatg ccgacattga ggtagcagca aggcgcattg catggggaaa
     1861 atatctaaat ggtggacaaa cttgtgtagc tccagattat ttattgttac acgaatccgt
     1921 aaaggaaaaa ttttacaaag catttactaa aacagtaaaa gaattttatg gcaataatcc
     1981 gcaagacagt gccgactatc cgagaataat caacgaccga cattttaaac gcatcgctgc
     2041 tatgattaca gacggcaata taattgttgg tggaaaaaca gatgcagaaa cgcgctacat
     2101 ttcaccgacg ctaattgaga ttgactcctt agcgcatccg ttgatgaatg atgaaatttt
     2161 tggccctgta ttgcctgtgc taacaataaa ttccattgac gatgccattc acattatcaa
     2221 gcaatatgaa aaaccattag cattttattt attctctaat aactattcaa atcagcaaaa
     2281 atgcttgcag actattcagt ttggtggcgg ttgcatcaat gatactattt cgcacctagc
     2341 caatcatgaa ttgccatttg gtggcattgg caatagtgga agcggtgcct atcatggcaa
     2401 agccagcttt gatacttttt ctcacaaaaa aagtatttgt cataaagtaa catggccaga
     2461 cgtgccattg cgatatccgc cgtataatgg aaaattgatg ctaataaaac aagtgatgaa
     2521 atagacttag atttggattt aatactccgc atagccgaat gagtaaggcg cgattgttat
     2581 cacatttcca gtgatatttt cggttttaat ggtgatcggt tttacttttt cccgatactt
     2641 cttttcgtac atcgttttac ctgccgttgc ccaaggtacg cttgcttcaa tcgagttgac
     2701 ttgtaccaat cctgatgttt tatctttaat aaaggtgagt tcaatttcct tagcagagcg
     2761 attaacgaaa tgcaaataag tagcattgtt ttgtgtgttg cgaaatactt gacatactaa
     2821 atttttatga atttctttgt cttcactcgt cattgatgtt tcacaacgat ttgtattttg
     2881 gcttagggta gtagctaaaa atttgaatgc aaaatattgc acgcttgcat tgtcatttcc
     2941 tttagagaaa acaggctttt gtttgccttt gaaaatagta atcaaaccgt ctattgcatg
     3001 caaactcgag atgtcgatgt aattattttg cttggcattt attgctatca tttccagaaa
     3061 ttgctcatac acaaaagcac cttgcacaaa ggtattatcc aaatattctc catttgatac
     3121 attccattct gtgagccaaa gttttctatt ttcgccatac aatgatttat aaattcgttg
     3181 catgtcttgt aattgattgg attgaaatgg cgctaaggct gttaaatttt tggaatatac
     3241 attttcaaaa aaatcgtcgt catcgcatgg cttacaggct gcataataat gaaaaatata
     3301 tccatcataa aaatttactt tactcaagcc tatattccac tgccgcatga aattactgcg
     3361 ttgcgtttta tcctcttctt ctgtcggaac accagcaact acggcaattt tcaaattcgg
     3421 atatttttgg cgaatggcag cggcggtgtt ttttatcttg tcgatatat
//
LOCUS       DAOQQV010000175         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_13862_length_3404_cov_6.139445, whole genome shotgun sequence.
ACCESSION   DAOQQV010000175 DAOQQV010000000
VERSION     DAOQQV010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13862_length_3404_cov_6.139445"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /gene="sucB"
                     /locus_tag="PLL59_12055"
     CDS             <1..678
                     /gene="sucB"
                     /locus_tag="PLL59_12055"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HMY24589.1"
                     /translation="RRLVAVKNETAMLTTFNEVDMTRIHEIRAQYKKDFEEKKGIGLG
                     FMSFFTKACAIALQKYPAINAYIDDENIIYHDFCDISIAVSTPKGLVVPVIRNAESLS
                     FAGVEKEIKRLALLGRDGSLSLQDMDGGTFTITNGGVFGSLMSTPIINAPQSAILGMH
                     KIQDRPMAINGKVEIRPMMYLALSYDHRIVDGKESVGFLVMVKDLLENPEKMLFGTDP
                     VKALLEL"
     gene            complement(717..1130)
                     /locus_tag="PLL59_12060"
     CDS             complement(717..1130)
                     /locus_tag="PLL59_12060"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HMY24590.1"
                     /translation="MKNKVVLILLLSLSSVYAQSPVGKWKKITHVSTFEGQTFDSHKA
                     LLIQRPCAAKFVYEINADATFRLNALQSGCDEKYTKVQEKLYSETKWKVEGNKITISS
                     IKDFAVGQSYIISFSGNKMIWKGTDGQGTITYQKL"
     gene            complement(1141..2016)
                     /locus_tag="PLL59_12065"
     CDS             complement(1141..2016)
                     /locus_tag="PLL59_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24591.1"
                     /translation="MKTPINYLIFLIIALISSCSNNNTPAPQPTPSNNSSKTVLYDAN
                     NIGDPDDDDYCFMLLGADNSGQKKYFKAKMIDIVKDSMEITEGPYTLDSLIDVSTFPS
                     YIKSASSGLYLTDFVFSDSIRVRQTGLNANGKRVFIQVPWLGNGSDYVKPYTKGAQIE
                     GTAIRNYFKKPTASGSFEGYLMHFFFKKGTCIIFSKTSASGTGVSVSGFPEDISSVIG
                     ASYDWENVDNYFNVTTGVGTSSHYFIDYKNWRYFRIKESKTSVPGTPGGQHAIEYGGY
                     KSLDKLLNWPSDWKN"
     gene            complement(2040..2618)
                     /locus_tag="PLL59_12070"
     CDS             complement(2040..2618)
                     /locus_tag="PLL59_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24592.1"
                     /translation="MKMKLLIFLMLSMSLLSCKKEEPDNPNINHRIVNLEMKVSSYNA
                     ESDMSIDMNDDGMQDFEIELDLYKDAEDDLDYELWLDYYQSGNEFLTQEINSDAYAKP
                     LNKNDLISGGSSTWESYCNFFELEKSPGYPDDNYGFAGKGDILVGVRFMIGTERHYGW
                     MKINASSDYKTVIVKEVAYEIRPNVEIRAGEK"
     gene            complement(2631..3152)
                     /locus_tag="PLL59_12075"
     CDS             complement(2631..3152)
                     /locus_tag="PLL59_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24593.1"
                     /translation="MKDLKFSINCICLLLVSLAFSACKKDKEPSSANHLSFKIDGVYW
                     ESDKEEVFGSYHLSEAIGPKLINIAGARGSGSTQQTFNINLFNTDVAGLYSVNIPIGA
                     TAGINENVAQFSNLTSTNYLCGGTLQGSQFNINVTKVSKSPQIVEATFSGFMECVDGN
                     TIVISEGKFIYHE"
BASE COUNT         1053 a          622 c          573 g         1156 t
ORIGIN      
        1 cgtcgtttgg tagctgtaaa aaatgaaaca gccatgctaa cgacgtttaa tgaagtagat
       61 atgacgcgca ttcacgaaat tcgtgcgcaa tacaaaaaag actttgaaga gaaaaaagga
      121 atcggcttag gctttatgtc atttttcacg aaagcatgcg cgattgcatt gcaaaaatat
      181 ccagcaatta atgcatacat cgacgatgaa aatattattt atcacgattt ctgcgatatt
      241 tctattgctg tttctactcc aaaaggctta gttgtgccag ttatcagaaa tgcagaaagc
      301 ctttcgtttg caggcgttga gaaagaaatt aaacgcttag cattattagg acgagatgga
      361 tctttgagcc tgcaagatat ggacggtggt acattcacca tcactaatgg tggcgtgttt
      421 ggctcattaa tgtcaacacc aattatcaat gcgccacaaa gtgctatatt gggtatgcat
      481 aaaattcaag atagaccaat ggcaattaat ggcaaagtag aaatacgacc aatgatgtat
      541 ttggcattat cttacgacca cagaattgta gatggcaaag aaagcgtagg gtttttagta
      601 atggtaaaag atttattgga aaatcctgaa aaaatgctat ttggaactga tcctgtaaaa
      661 gcattactgg aattgtaaaa atcgattaca aaaagggtat tcataagagt acttttttat
      721 aatttttgat aagtaatagt accttgtccg tctgttcctt tccaaatcat tttatttccc
      781 gaaaaggaaa ttatatagga ttgtcctaca gcaaaatctt ttatcgatga aatggtaatt
      841 ttattgcctt ctaccttcca cttggtttcg ctatacaatt tttcctgtac tttcgtatat
      901 ttttcatcgc atccgctttg taatgcattt aatctgaacg tggcatccgc atttatttca
      961 tacacaaatt ttgcagcaca tggtcgttgt ataagcaatg ctttgtgtga gtcaaatgtt
     1021 tgaccttcaa aagtagaaac atgagttatt tttttccatt ttccaacagg actttgcgca
     1081 taaacactgc ttagggaaag caataaaatt aatactactt tatttttcat tgtaatgatt
     1141 ttaatttttc caatcgcttg gccaatttaa taatttatct aaacttttgt agcctccata
     1201 ttctatggca tgttgtccgc ctggtgtacc cggcacactg gttttactct cttttattcg
     1261 gaaataacgc cagtttttat aatctataaa ataatgtgat gaagtgccta caccagtggt
     1321 tacgttgaaa taattatcaa cattttccca atcataagat gcaccaatta cggatgaaat
     1381 atcttctgga aaacccgaca cagaaacgcc tgtgcctgat gcacttgttt ttgagaaaat
     1441 tatacatgta cctttcttaa aaaagaaatg cattaagtat ccttcaaaac ttcctgatgc
     1501 agtaggcttt ttgaaataat ttcgaatagc tgttccttct atttgagctc cttttgtata
     1561 aggttttaca tagtctgatc catttcctaa ccatggtact tgtataaata ctcttttccc
     1621 attagcattt aatcctgttt gtcgtactcg aatactatca gaaaaaacaa agtctgttag
     1681 gtacaagcca gaacttgcag attttatgta agaagggaaa gtgcttacat ctattaaact
     1741 atctaaagta taaggacctt ctgtgatttc cattgaatcc ttaacgatat ctatcatttt
     1801 tgctttaaag tatttttttt gacctgagtt atctgcacca agtaacataa aacaatagtc
     1861 atcgtcatca ggatcaccaa tattgtttgc atcatacaat accgttttag atgaattatt
     1921 agatggtgtt ggttgtgggg caggtgtgtt gttattggaa caactcgata tgagtgctat
     1981 tattaagaat attagatagt taattggcgt cttcatgtgt caatattttt ttagtttatt
     2041 tatttttctc cggctctgat ttctacattt ggtctgattt cgtatgccac ttctttaaca
     2101 ataactgtct tataatctga agaagcattg attttcatcc agccataatg acgttcagta
     2161 ccaatcataa aacgaacacc aactaaaata tcacctttgc ctgcaaagcc ataattatca
     2221 tcaggataac ccggcgattt ttcaagctca aagaaattgc aataactttc ccaagttgaa
     2281 gagccaccac tgattaaatc atttttattg agcggttttg cgtatgcatc cgaattgatt
     2341 tcctgcgtaa ggaattcatt gccactttga taataatcca accataactc ataatcaaga
     2401 tcgtcttcag catctttata taaatccagt tctatttcaa aatcctgcat accatcgtcg
     2461 ttcatatcaa tgctcatatc agattctgca ttgtatgaac tgactttcat ctcgagatta
     2521 acgattcgat ggttgatgtt tggattatcg ggttcttcct ttttgcaaga cagcaaagac
     2581 atgctcagca ttaaaaaaat caataatttc attttcatag ttctatgatt ttattcgtga
     2641 taaataaatt ttccttctga aattacaatg gtgttaccat ctacacattc cataaaccca
     2701 ctgaatgtcg cttcaacaat ctgagggctt ttagatactt ttgtcacatt aatattaaat
     2761 tgactgcctt gcagagtgcc gccacataaa taattggtag atgtaaggtt ggaaaattgt
     2821 gctacatttt cgtttatacc ggcagtagca ccgattggaa tatttacgct atacaaaccg
     2881 gccacatctg tattaaagag attaatgttg aatgtttgct gcgtcgaacc acttcctctt
     2941 gcgcctgcta tattgattaa tttggggccg attgcctcag acagatgata actgccaaag
     3001 acttcctctt tgtcggattc ccaataaaca ccgtcaattt taaagctcag gtgatttgcc
     3061 gaggatggtt ctttatcttt tttacaggct gaaaatgcaa gtgacaccag cagtagacaa
     3121 atgcaattaa tcgaaaattt taaatctttc atagcttagt gatataaggt attacccaaa
     3181 attgatttaa ttattttaac gaattagccg tgaatcgacc aacgacattt tttatcgaca
     3241 aacaacattt atttcttatt ttagtactat aatgggttac tcgtacgtca tagtagaaga
     3301 taacgaactg gacaggcttt ctctaaaagt attccttaaa tcctatagag actttgttgc
     3361 tgcaggtgaa tatacttccg ccgaagaagc cctgattcca atca
//
LOCUS       DAOQQV010000176         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_14886_length_3254_cov_7.847140, whole genome shotgun sequence.
ACCESSION   DAOQQV010000176 DAOQQV010000000
VERSION     DAOQQV010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14886_length_3254_cov_7.847140"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /gene="eno"
                     /locus_tag="PLL59_12080"
     CDS             complement(<1..526)
                     /gene="eno"
                     /locus_tag="PLL59_12080"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585660.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMY24594.1"
                     /translation="MSHIISVHARQILDSRGNPTVEADVVTSNGVLGRAAVPSGASTG
                     THEAVELRDGGKAYMGKGVGKAVNNVNTFIAEALVGENVLQQREIDTILLELDGTENK
                     KKLGANAILAVSMAAAKAGAQEAGLPLYRYIGGTNARTLPIPLMNILNGGAHADNKID
                     FQEFMIVPVGATTFS"
     gene            complement(602..1354)
                     /locus_tag="PLL59_12085"
     CDS             complement(602..1354)
                     /locus_tag="PLL59_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMY24595.1"
                     /translation="MDYKHRFSDRVENYIKYRPCYPKEVITTLKAEIDLKPEDIVADI
                     GSGTGLSAKIFIENGNTVYGVEPNEPMRKAAEGLLCEYDNFISVHGSSEASNLAANSI
                     DLIVCAQAFHWFDRKKTKKEFQRIANNGAHLCLLWNDRKASEPFQRDYENIIQEFATD
                     YNDITHRNITNEIITEFYAPNAFKKMVLQYEQHFDLEGLIGRIVSSSYMPNEKDDNFP
                     QLRNAIVDLFNAYKQNELVTFAYNTILYIGKC"
     gene            1771..>3254
                     /locus_tag="PLL59_12090"
     CDS             1771..>3254
                     /locus_tag="PLL59_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY24596.1"
                     /translation="MKITIIGNGMVGQRLCEKLVDLKMPNLSLSVFGEEPITAYDRVH
                     LSEYFAGKSVKELCIKPMSWYKENNIQLYLGNPILEIDRDSQCVTALEGQIVYYDYLI
                     IATGSAPFIPNMYGTDKSGVFMYRTIEDLNMIEKYAASCKTGVVIGGGLLGLEAAKAL
                     LDLGIKDIKIVEFSQRLMPRQIDNEGSDALKNKLKELGITTLNNKNTMAILGNESIHS
                     LIFADGSTLSTDIAIISAGIKPRDELARKCGLDIGPRGGIIVNDNMQTSDPNIYAIGE
                     CALHDNIIYGLVAPGYEMAEVVAQQLIKNKNKAFIGFDMSTKLKLIGVDVASFGDPFI
                     DGNDAKCIVYNDTLSGTYKRINISNDGKYLLGGSLVGDTEEYNKLLQIFKNKMEVKGN
                     PLHLIVKSDNSNANEGFELPDDALICSCEGVTKKDISDAVEIQGCENFDAVKKCTKAG
                     TGCGGCVPMVKDIVNNSLKKQGKVIKKVICEHFQYSRQELYSLI"
BASE COUNT         1099 a          566 c          554 g         1035 t
ORIGIN      
        1 cagaaaatgt agttgcacca acaggcacaa tcataaattc ttgaaaatcg attttattat
       61 cagcatgtgc accaccattt aaaatattca tcaaaggaat gggcaatgtg cgtgcattcg
      121 tgccaccgat atagcgatat aatggcaagc ctgcttcttg tgcaccagct tttgctgccg
      181 ccattgacac tgctaaaatg gcatttgcgc ctaacttttt cttgttttca gtgccatcta
      241 attccaataa aatggtatcg atttctcgct gttgtaaaac attttctcca actaatgctt
      301 ctgcaataaa agtgtttaca ttgtttaccg ctttgcccac acctttaccc atgtaagctt
      361 taccaccatc gcgcagttct actgcttcgt gtgtgcctgt tgatgctcct gaaggcactg
      421 ctgcacggcc aagtacacca ttgctggtca ccacgtctgc ctctactgtt ggatttcctc
      481 ttgaatctaa aatttgtctt gcatgtactg aaataatgtg actcatgttt tgattttttt
      541 aatgatagtt atcatcagaa aattgcacta attgtcaaaa acaacataca aaatctaatg
      601 attaacattt tccaatgtat aaaattgtgt tataggcaaa agtaactaat tcattttgtt
      661 tatacgcgtt aaataaatca acgatagcat ttcttaactg tggaaaatta tcgtcttttt
      721 cgtttggcat ataagatgaa gatacaatac gacctattaa gccttctaaa tcaaaatgtt
      781 gctcgtattg tagtaccatt tttttaaatg cattaggcgc atagaattca gtaataattt
      841 cattggtgat attacggtgt gtaatatcat tgtaatctgt tgcaaattct tgaataatat
      901 tttcataatc tctttgaaac ggctcacttg cttttctatc attccaaagt aaacataaat
      961 gcgcaccatt attggctatt cgttgaaatt cctttttcgt ttttttcctg tcaaaccaat
     1021 gaaatgcttg tgcacaaact attaaatcga tgctgtttgc tgctaaattg cttgcttctg
     1081 aactgccatg cacactgatg aaattatcat attcgcaaag taagccttcc gctgctttac
     1141 gcattggttc attaggctcg acaccataaa cagtattgcc attttctata aatatttttg
     1201 cagacaagcc agtgccactg cctatgtcag ctacgatatc ctcaggcttg aggtcaattt
     1261 ctgcttttag tgttgtaata acttccttag gatagcaagg tctatatttg atgtaatttt
     1321 ctacgcggtc agaaaatctg tgtttgtaat ccattttttt cttgatacta attgttgaat
     1381 aaatttacaa aaatcatagt gtattcaaat atgattacat acatgtttca cgacctaaag
     1441 gtggattata ttttttacca acaactaatt ccaattgcaa catttactat tgcatttatt
     1501 tcttgaattc tgcattgcaa acagtgttaa gatgaaatac tgttcaatag cattcaactc
     1561 aattgatttt tttgaattac ataatgcgta gataacatct tataattata aaaatgtaat
     1621 aaattttatg gtaagataat aataaatcga tttaagtaat gaaattatag cataatttca
     1681 caaacatcaa atcgaattat cgaaataatc ttaaaattat tgtgaaataa aaaaagtctc
     1741 tactaattaa atatttaaaa actcacactt atgaaaatta caattatcgg aaatggaatg
     1801 gtaggacaaa gactttgcga gaagctagtt gacctaaaga tgcctaattt atctttaagc
     1861 gtattcggag aagaacccat cactgcttat gacagagtac acctaagcga atatttcgca
     1921 ggcaaatctg ttaaagaact ttgtatcaaa ccaatgagtt ggtataaaga aaacaatatt
     1981 caactgtatc taggaaatcc tattctagag attgaccgtg atagtcaatg cgttactgca
     2041 ttagaaggac aaatcgttta ctatgactac ttaattattg ctactggctc tgcaccattt
     2101 atacctaata tgtatggcac tgataaatcg ggtgtattta tgtataggac tatcgaagac
     2161 ttaaacatga ttgagaaata cgcagcatcg tgcaagacag gtgtcgtaat tggaggtggc
     2221 ctattaggat tagaagcagc aaaagcatta ttagatttgg gcattaaaga cattaaaatt
     2281 gtagaatttt ctcaacgttt aatgccaaga caaattgaca acgaaggctc tgacgcttta
     2341 aaaaataaac taaaagaatt aggcatcaca acgctcaaca ataaaaacac gatggcgatc
     2401 ttgggtaatg agtccattca tagtctgatt tttgcagatg gttctactct atccacagac
     2461 attgctatca tatctgcagg cataaaacca agagacgaat tagctagaaa atgcggatta
     2521 gacattggcc cgcgtggtgg cataattgta aatgacaata tgcaaaccag tgatccaaat
     2581 atatatgcta ttggagaatg tgcgcttcat gataatatta tttatggttt ggttgcacct
     2641 ggctatgaaa tggcagaagt tgtagcacaa cagctaatta aaaacaaaaa taaagcattt
     2701 atcggttttg acatgagcac taaactaaaa ttaatcggtg tagatgttgc cagttttgga
     2761 gaccctttca ttgatggaaa tgatgcaaaa tgcattgttt acaatgacac gctctccggc
     2821 acctacaaac gtataaatat ctctaatgac ggtaaatatc tattaggagg tagtttagtc
     2881 ggcgatacag aagaatacaa taaactatta cagatattca aaaacaaaat ggaagtaaag
     2941 ggaaatccac tccatttaat agtaaaatca gacaattcaa acgctaatga aggatttgaa
     3001 ttgccagacg atgctttgat ttgtagttgt gaaggtgtca caaaaaaaga catctcagat
     3061 gctgttgaaa tacaaggttg cgaaaacttt gatgcggtga aaaaatgcac aaaagctgga
     3121 acaggatgtg gtggctgcgt accaatggta aaagatattg taaacaactc actaaaaaaa
     3181 caaggcaaag tcataaaaaa agtaatctgc gagcattttc aatattccag acaagaacta
     3241 tatagcctca taca
//
LOCUS       DAOQQV010000177         3226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_15103_length_3226_cov_6.869442, whole genome shotgun sequence.
ACCESSION   DAOQQV010000177 DAOQQV010000000
VERSION     DAOQQV010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15103_length_3226_cov_6.869442"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLL59_12095"
     CDS             complement(<1..900)
                     /locus_tag="PLL59_12095"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HMY24597.1"
                     /translation="MQELIDLVSKETLIDKKQVVATIQLLDEGASIPFIARYRKEKTD
                     SLDEVEIGLIKNSIQKYRDIEKRKEAILKSIEDQGKLSDDLKEKITSCYDVLILEDIY
                     LPYKPKRKTKATVAKEKGLEPLATLIYKQQERNVIAKAETFITDEVKSVEEALQGARD
                     IIAEWINEDMIVRSLVRQQFERHAIVKTKIAYGKEEDKEAQKFRDYFEWEEPLKHCPS
                     HRMLAMRRGEMEGFLYFHIAPDEEQMLDILQHRIVKGNTAAAEQVAIALKDAYKRLIK
                     FSIELEFRNIAKEKADKEAIDVFV"
     gene            complement(1057..3183)
                     /locus_tag="PLL59_12100"
     CDS             complement(1057..3183)
                     /locus_tag="PLL59_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMY24598.1"
                     /translation="MFKYEKDNDNIVTVSMDMQGRSANVINEAFGKLWLEMIDRLEKE
                     KNDITGVVLTSAKSTFFAGADIDNLFKQSDAKLIFEMCEGLKAQLRRLEILGKPVVAA
                     LNGAALGGGLEIALACHYRIALNYPKAQFGLPEVGLGLLPGGGGIIRLTRMVGLQPAL
                     PLLTEGKRLKVKEALSNGIINEIAETPEDMMAKAKAWIKANPKAINPWDDKAFKFPGG
                     DAKNPKVAGMIPIVPAMTKKKTHGNYPAVEAIINTAVEGSVVDFDTACRIESRYFTSL
                     AVGQVSKNMISAFWYNLNAINGGGSRPKGFEKYTTKKVGVLGAGMMGAGIAYVTAISG
                     MEVVLKDVSIEAAEKGKAYSEGLLKKRVAKGRMTKEKADEVLARIKTTDKPEFDGCDL
                     IIEAVFEDRALKAKVTQEAEASMLAEGTFASNTSTLPITGLATASSRPKQFIGLHFFS
                     PVDKMPLVEIICGAATDDTTLAKAFDYVLQIKKTPIVVNDSRGFYTSRVFSTYVMEGI
                     AMLGEGQHPRAIENAGLKAGMPVGPLALTDEVSMKLMDHIRRQTEQDLIAEGKPIPTH
                     PGFAVCEKLLNANRPGKAAGAGFYEYPQGGTKYLWPELTNMFPQQNALDQQEMVDRIL
                     IVQALDTVRCLEEGVLRSVADANIGSIFGWGFSPFNGGTLQYINGQGLKNVIEKAKQL
                     EAKYGERFAVPKLLQDKADKGELFVD"
BASE COUNT          873 a          665 c          515 g         1173 t
ORIGIN      
        1 tacaaataca tcaatcgcct ctttgtctgc tttttctttt gcaatatttc taaattctaa
       61 ttctatcgaa aattttatca aacgtttgta agcatctttc aaagctattg ctacttgctc
      121 agctgcagct gtatttcctt tgacaatccg atgttgtaaa atgtctaaca tttgttcttc
      181 atctggcgca atatgaaagt aaagaaagcc ttccatttca ccacgacgca tggctaacat
      241 acgatgtgaa ggacagtgtt ttaatggctc ttcccattcg aaataatctc taaatttttg
      301 tgcctcttta tcttcttctt ttccatatgc tatttttgtt tttacaatgg catgtctttc
      361 aaattgctga cgtactaaac ttcgtacaat catatcttcg tttatccatt cagcaatgat
      421 atcgcgagcg ccttgcagtg cttcttctac gcttttgact tcatctgtaa taaaagtttc
      481 agcctttgca ataacattgc gttcttgttg tttatagatt aatgttgcca atggttctaa
      541 gcctttttct ttagcaactg ttgcctttgt tttcctttta ggcttgtatg gtaaataaat
      601 gtcttctaaa ataagcacat cataacaaga tgtaattttt tcttttaaat catcagacag
      661 cttgccttgg tcttcaatgg attttaaaat agcttctttg cgcttctcaa tgtctctata
      721 tttttggata ctgtttttaa tcaaaccaat ttcaacttca tctaagctgt ctgttttttc
      781 ttttcgatat cgtgcaataa atggtatcga tgcaccttca tctaataact gaatagtggc
      841 aactacttgc tttttatcta ttaaggtttc tttagaaact aaatctatta attcttgcat
      901 attttatatt gaaaggctgc aaaaatagag taaaaatcaa gatagcaaaa tgaaacagga
      961 agattgttat taacattctt atagatgatt ttttaataat cataaaataa taaagaccga
     1021 gtaattactc ggtctttatg catacttgtg atataattag tctacaaata gctcaccttt
     1081 gtcagctttg tcttgcagta attttggcac agcaaaacgc tcgccatatt ttgcttctaa
     1141 ttgcttcgct ttttcaatta cattttttag gccttgaccg ttgatatatt gtaatgttcc
     1201 accattaaaa ggagaaaaac cccaaccaaa tatagaaccg atgttagcat ctgctacact
     1261 tcgcaaaact ccttcttcta aacaacgaac tgtatctaat gcttgcacta ttaatattct
     1321 atccaccatt tcttgttggt ctaaagcatt ttgttgtgga aacatatttg tcaattctgg
     1381 ccataaatat tttgttccac cttgcggata ttcataaaat ccagcacctg ctgctttgcc
     1441 aggtctgttt gcgtttaata atttttcgca aacagcaaat cctggatgtg taggaattgg
     1501 ctttccttct gcaattaaat cttgttctgt ttgtctgcgt atatgatcca ttaatttcat
     1561 gcttacttca tctgttaatg ctaatggacc aaccggcata cctgctttca atccagcatt
     1621 ttcgattgca cgtggatgtt gtccttcgcc taacatggct atgccttcca ttacataagt
     1681 agagaatact cgtgatgtgt aaaatccacg agaatcgttt acaacgattg gtgttttttt
     1741 aatttgtaac acataatcga aagctttggc taaagttgtg tcatcagttg ctgcgccaca
     1801 aataatttct actaatggca ttttgtcaac aggcgaaaag aaatgcaaac cgataaattg
     1861 ttttgggcga gaagacgctg ttgccaatcc tgttattggt aacgttgatg tatttgaggc
     1921 aaacgtacct tctgctaaca tggatgcttc agcctcttgt gttactttag cttttaatgc
     1981 tcggtcttcg aacactgctt cgataattaa atcgcaacca tcaaattcgg gcttgtctgt
     2041 ggttttaatc ctagcaagca cttcatctgc tttctctttt gtcattctac cttttgcaac
     2101 acgttttttc aacaagcctt cagagtatgc ttttcctttt tcagcggctt caatgctgac
     2161 atctttcaat acgacttcca tgcctgagat tgctgtaaca taagcaattc ctgcacccat
     2221 catacctgca cctaatacac ctactttttt agtcgtatat ttttcaaatc cttttggtcg
     2281 agagccacca ccattaatag catttaagtt ataccaaaat gcagaaatca tatttttaga
     2341 tacttgacca actgccaatg atgtaaaata acgcgattcg attcggcaag cagtatcaaa
     2401 atcaacaaca gagccttcta ctgccgtatt aataattgct tctactgctg gataattgcc
     2461 gtgtgttttc tttttagtca tcgccggtac aatcggaatc atacctgcta cttttggatt
     2521 tttggcatcg ccacctggaa atttaaaggc tttgtcgtcc caaggattaa tggcttttgg
     2581 attggctttt atccaggctt tcgcttttgc catcatatct tctggcgttt cagcaatctc
     2641 gttgatgata ccattgctca atgcttcttt tacttttaat cgtttgcctt ctgtcaataa
     2701 tggtaatgct ggttgcaaac ctaccattct tgtaagacga ataattccgc caccgccagg
     2761 caacaaaccc aaaccaactt ctggtaaacc aaattgtgct ttcggatagt tgagcgcaat
     2821 gcgataatgg caagctaagg caatttctaa tccaccgcca agtgctgcgc cgttaagtgc
     2881 tgcaactact ggttttccta agatttccaa gcgacgaagt tgagccttca agccttcaca
     2941 catctcaaaa attaatttcg catcgctttg tttgaataga ttgtcgatat cagcacctgc
     3001 gaagaaagta gattttgctg atgttaaaac tacgccagta atgtcatttt tttctttctc
     3061 taatctgtct atcatttcca gccatagttt accgaatgcc tcgttaatta cattggcact
     3121 tctgccttgc atatccatcg atactgttac gatgttatcg ttgtcttttt cgtatttaaa
     3181 catattttag tattaagtag tgagtaataa gtattgagat tttttt
//
LOCUS       DAOQQV010000178         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_15151_length_3222_cov_7.035996, whole genome shotgun sequence.
ACCESSION   DAOQQV010000178 DAOQQV010000000
VERSION     DAOQQV010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15151_length_3222_cov_7.035996"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLL59_12105"
     CDS             complement(<1..231)
                     /locus_tag="PLL59_12105"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HMY24599.1"
                     /translation="MQKRVVVGLSGGVDSSVTAYLLQQEGYEVIGLFMKNWHDGSVTI
                     QGDCPWIDDSNDALLVAQKLGIPFQTLDLSSEY"
     gene            complement(345..2756)
                     /locus_tag="PLL59_12110"
     CDS             complement(345..2756)
                     /locus_tag="PLL59_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMY24600.1"
                     /translation="MLSKLSIDSRTKNSGSIIEGEVIKQSFCWNTDKSSIFTINTIEV
                     SKILKGSASATIDVITPGGEIDGKLLVVEPNASLSVGSKGVFFLMPSIKSLNYSSNYP
                     KYEIFGLAQGFIQLDERTGSYSDPFEDYPSKNNVYSAIQKTTGINYRETAPLINNIET
                     GEASISLFTPASISAGTQSVLTISGWGFGSRTGSATVQFRDANSTSSAMYTNIPDTSY
                     ILSWTDTEIKVIVPGASINRQGGAGSGAFNVITANGTAISSIAPLSITYNKFEYKKNK
                     IALYNQNGQGGYTFTLNTDFDNNALAKAAFLKGLNQWKCNTSVNIAINPTSSSNACNN
                     QMDNINLISFSTPACPLPAGALAVTYSSYTLCGNSPIIPDGIDMIFSAGANYYFGSDV
                     TPPNQYDFESVVLHELGHAFGQGHHSEYTEIMFPSIANGVMKRTLKETSDIASVNETI
                     DLSTATSYCGFQKHVRANTSNCNTNTQIAPISSQFIADRTTGCAPFTVKFTDQSTGNP
                     TSWRWDINNNGSTEYTTQNPSHTFTSAGTYTIKMVALNTTSRDSIIKTSVITVSPALA
                     ANIDVVQAITCNGNNNAILKVTPSGGNGNYTYQWNNNNTNQTISNISAGTYTVTLKDG
                     NNCIVTKSKDITQPEKIKVNVNTELVNNNYTATLDVSGGTQPYMYILNNATQFANNII
                     PNLSAGNYNVVVKDNNNCIQSASFSVEATTGIIEAEQSFDNLDIFPNPATNNINIHFS
                     LKEYKSIKLSLMDLSGQTIFQENYNDIKDKQANLDLSSLSSGTYIIRLELPEGNTFRK
                     IIISR"
     gene            2948..>3222
                     /gene="miaE"
                     /locus_tag="PLL59_12115"
     CDS             2948..>3222
                     /gene="miaE"
                     /locus_tag="PLL59_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HMY24601.1"
                     /translation="MLGLKLATDPRWVNLASLSIEEILTDHAFCEQKATTNCISLIQL
                     YPEKTFLVDKLIPIVNEEWSHFKMVHDELKKRNLKLGKQRQDKYVVA"
BASE COUNT          995 a          539 c          550 g         1138 t
ORIGIN      
        1 gtattcacta cttaagtcga gtgtttgaaa aggaatgcct aatttctgcg ctaccaatag
       61 tgcatcattg ctatcgtcaa tccatggaca atcgccttga atagtaacgc tgccatcgtg
      121 ccagtttttc ataaacaagc ctattacttc atagccttct tgctgtaata aataagcagt
      181 aacacttgaa tctacaccac cagataagcc tacaacaaca cgtttttgca tgttgcaaaa
      241 ttacaaattt cgctgtatga tattattatc taaataaaaa ataaacccac agtagaaacc
      301 gcaggtctta acataacatt cattaaataa ccaaaaaata ttttttatct gctgataatt
      361 atctttctaa atgtgttgcc ttcaggcaat tctaaacgta taatgtaagt gcctgatgat
      421 aagctggata aatccaaatt ggcttgtttg tcttttatat cattgtaatt ttcttgaaaa
      481 atagtttgac cagataaatc cattaaagac aatttaatag acttatattc tttcaaagag
      541 aagtggatat ttatattgtt tgttgctgga tttggaaaaa tatctaaatt atcaaagctt
      601 tgttccgcct caataattcc tgttgttgct tctaccgaaa atgaagcact ttgaatacaa
      661 ttattattgt cttttactac aacattataa tttccagcag ataaattggg aataatattg
      721 tttgcaaatt gtgtagcgtt attcaatata tacatatacg gttgtgtgcc accgctgaca
      781 tcaagtgttg ctgtataatt attattcacc aactcagtat tgacgtttac ttttattttt
      841 tcaggttgtg taatgtcttt agattttgtc acaatgcaat tattaccatc tttcaatgtt
      901 acggtataag tgccagctga aatgtttgaa atggtttgat tagtgttatt attattccat
      961 tgatacgtat aattgccatt tccaccgctt ggtgttactt ttaagatggc attgttgttg
     1021 ccattacagg taatggcttg cacgacatct atgttcgctg ccaatgctgg agatactgta
     1081 attacagatg ttttaataat tgaatcgcga gatgtggtgt ttaatgctac catttttatg
     1141 gtataagttc cagcgcttgt aaatgtatgt gatggatttt gtgtcgtata ttcagttgaa
     1201 ccattattgt tgatgtccca tctccaagaa gttggatttc cagtcgattg gtcggtaaac
     1261 tttactgtaa atggcgcaca acctgtagtt ctgtctgcaa taaactgtga tgatatcggt
     1321 gcaatttgtg tgtttgtatt acaattgctg gtatttgctc ttacatgttt ttgaaaacca
     1381 caatacgaag tagccgtgct caaatctatc gtctcattca cactggcaat atcagatgtt
     1441 tcttttagtg tacgtttcat tacaccattt gcaattgatg gaaacatgat ttctgtatat
     1501 tctgaatgat gtccttgtcc aaatgcatgg cctagctcat gtaaaacaac actctcaaaa
     1561 tcatattgat tgggtggcgt aacatcgcta ccaaaataat aattcgcacc tgcgctaaat
     1621 atcatgtcta taccatcagg aataataggt gagttgccac ataaagtgta agaagaatag
     1681 gtaacagcta atgcaccagc tggtaatgga caagctggtg ttgaaaaaga aattaagttg
     1741 atattatcca tttggttatt acaagcattg ctactgcttg ttgggtttat ggcaatattc
     1801 acactagtat tgcatttcca ttgattaagt ccttttaaga atgcagcttt agctaaggcg
     1861 ttattatcaa aatcagtatt taatgtaaat gtatatccac cttgtccgtt ttgattgtat
     1921 aatgcgattt tatttttttt gtactcaaat ttattataag taatacttaa tggtgcaata
     1981 gaagaaatag ctgtaccatt tgcagtaatt acattaaaag caccagagcc tgctccacct
     2041 tgtctgttaa tagaagcgcc aggaacgatt acttttattt ctgtatctgt ccatgataaa
     2101 atataagatg tatccggtat attagtgtac atggctgaag atgtactgtt tgcatctcta
     2161 aattgcacag ttgcactacc agttctgctg ccaaaacccc aaccgcttat agtcaacacg
     2221 ctttgtgtac ctgctgaaat acttgcaggt gtaaataatg aaatagaagc ttcgccagtt
     2281 tcaatattat ttattaatgg cgctgtttct ctatagttta ttcctgtagt tttttgaatg
     2341 gcactataaa cattattttt acttggataa tcttcaaatg ggtcgctgta gcttcctgtt
     2401 ctttcatcta attgaataaa accttgagcc aaaccaaata tttcgtattt aggataattg
     2461 ctagagtagt taagtgattt tattgaaggc attaagaaga acacaccttt tgagcctact
     2521 gataaacttg cattaggttc aacaactaat aattttccat ctatttcgcc accaggtgtg
     2581 atgacatcga tagtagcaga agcgctgcct tttaaaattt tgcttacttc aattgtatta
     2641 atagtaaata tcgatgactt atcagtattc caacaaaagc tttgtttgat tacttcacct
     2701 tctattatac ttcctgagtt cttagtacga ctatcgatgc ttagcttcga cagcatacaa
     2761 tttgcgttgc aacaatagct gtacgctatt gcgaacaata ataaataggg tttcatgtta
     2821 tgttatttat gtttcaaaaa atagggtaat gcaaacttaa taaacatttt ctaaattgtc
     2881 aatagctatt ttacatattg ttaatattgt agtgaataaa tcaccttaaa caatgtaatt
     2941 ttgtcacatg ttaggattga aattagcaac tgacccacgt tgggtgaact tagcatcgct
     3001 ttccatcgaa gaaattttga ccgatcatgc cttttgtgag caaaaagcaa cgacaaactg
     3061 tatttctttg atacaattat atcctgaaaa gacattttta gtagataaat tgattcctat
     3121 tgtcaatgaa gagtggtctc attttaaaat ggtgcacgac gaattgaaaa agagaaattt
     3181 aaagttggga aagcaaaggc aagataaata tgtggtggca tt
//
LOCUS       DAOQQV010000179         3091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_16186_length_3091_cov_6.723650, whole genome shotgun sequence.
ACCESSION   DAOQQV010000179 DAOQQV010000000
VERSION     DAOQQV010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16186_length_3091_cov_6.723650"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..633)
                     /locus_tag="PLL59_12120"
     CDS             complement(136..633)
                     /locus_tag="PLL59_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMY24602.1"
                     /translation="MNIKTSITAIFVCLMTIFAQAESNYTIQNNALILPADIKFDQHD
                     YDIKDKTEPMLDFVAQYLKDRPYITKLRIEGHVFSEKSPEENIKLSLQRAAMVSYYLT
                     TKGIDCSRLIPVAFGDTKPVAPNDECNLTYNTRIDFFNAEINHKAIYGSVPNGFALKY
                     MEPCE"
     gene            complement(752..2425)
                     /locus_tag="PLL59_12125"
     CDS             complement(752..2425)
                     /locus_tag="PLL59_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMY24603.1"
                     /translation="MNWHHLFRKKPIDTILKEANQHPDLIESHGNAESLKRDLGVKDL
                     TALGIAAVVGAGIFSTIGKACFDGGPAVSLLFVGIAIACGFSALCYAEFASRIPIAGS
                     AYTYSYAVFGELIAWVIGWDLLLEYAIGNVTVAISWSGNFTNFLHGFGIELPRYLTTS
                     FLEAKHNMPELWAAAPKLFGIPFIINIPAFVINIFITYLVYVGIKESKNFANGMVLFK
                     VGVILVVIAIGIFYVVPGNWTPFAPNGVSGVLKGVSAVFFAYIGFDAISTTAEECKNP
                     QRDLPRGMIYALLISTVLYIAVALVLTGMVHYTHLNTEAFLATAFKDIGLDWIGGLIA
                     FSALIATASVMLVFQIGQPRIWMSMSRDGLLPEKFSTIHPKYKTPTFATIVTGLLVGI
                     PSLFLDAALVTDMCSIGTLFAFILVCGGVIILQDKDKKQENNVLISSPKFKTPYINGQ
                     YILPILVAIGLILFFKYPDNVQSTFPFDKGYDEWKHQIPFWIFIIVVIVMNVQTFIHK
                     WSIIPVLGFLSCSYLVSTLGVVNWMRFLVWMALGLLIYFIYGIRKSKLR"
     gene            complement(2488..2560)
                     /locus_tag="PLL59_12130"
     tRNA            complement(2488..2560)
                     /locus_tag="PLL59_12130"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2525..2527),aa:Lys,seq:ctt)
     gene            complement(2585..>3091)
                     /locus_tag="PLL59_12135"
     CDS             complement(2585..>3091)
                     /locus_tag="PLL59_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C peroxidase"
                     /protein_id="HMY24604.1"
                     /translation="NSTFDKYMRGEIHEINAAVKRGFNLFMGKAQCGSCHFAPTFFGL
                     VPPFYGVSEAEVLGITTSFDTINPVLDDDIGRFKNFAFDQFKFAIKTSTVRNVDITAP
                     YMHNGGFKTLEEVVEFYEHGGGAGMKLNVPNQTLPDKRLQLTTQEKKDIVSFMKALTD
                     TSGLSILF"
BASE COUNT         1076 a          586 c          473 g          956 t
ORIGIN      
        1 attaaccaaa aaatttaatt attgaatcga aatttacccg gacagatcta aggtagtgca
       61 tagaaaccct atacaaaaaa atcccatcac tattaagtat gggatttttt tttttaagac
      121 agtactgtat tattcttact cacaaggttc catatatttt aaagcaaaac cattaggaac
      181 actgccatat atggctttat gattaatttc agcattaaaa aaatcaatcc ttgtattata
      241 ggtaaggttg cattcatcgt taggtgcaac tggtttggtg tctccaaatg caactggaat
      301 taatctgcta caatcaattc ctttagttgt taaataatag ctcaccattg ctgcgcgttg
      361 taaactcagt tttatatttt cttcaggtga tttttcagaa aacacatgac cttcaattcg
      421 taatttagtg atgtatggtc tatctttaag atattgagca acaaaatcta acattggctc
      481 tgttttgtct ttaatatcat agtcatgttg atcgaacttg atatcagctg gtaaaattag
      541 cgcgttattt tgtattgtat aattagattc ggcttgcgca aatattgtca ttaaacaaac
      601 aaatattgct gtgatgcttg tttttatatt catttactag gttttacaga tgtaaatatc
      661 ttggaatatt ctgaattgtc caaccctgaa aatgttagaa tttagtgaaa aatgctgttt
      721 tgtatgtaga aatttaataa aataaaaaat attatctcaa tttactctta cgaatgccat
      781 agataaagta aatcagaaga cctaatgcca tccatactaa aaatctcatc caattaacga
      841 caccaagtgt agaaactaaa taggaacaag ataaaaatcc taatacagga atgatagacc
      901 atttatgaat aaacgtttgg acattcatca caataactac gataataaaa atccaaaatg
      961 gaatttgatg tttccactca tcataacctt tatcaaatgg aaaagtcgat tgtacattgt
     1021 ctggatattt aaaaaataaa atcaaaccaa tcgctacgag tattggcaat atatattgtc
     1081 cgttgatata aggcgtttta aatttcgggc ttgaaattag tacattattt tcctgtttct
     1141 tgtctttatc ttgcaagatg atgacgccgc cacaaactag aataaaagca aataaggtac
     1201 caatactaca catatctgtc actaatgcag catctaaaaa aagagatgga ataccaacca
     1261 acaaaccagt aacaattgtt gcaaatgttg gtgttttata tttaggatga atagttgaaa
     1321 atttctcagg caataagcca tcacggctca tgctcatcca tattctaggt tgtcctattt
     1381 gaaaaacaag cattacactg gcagttgcta ttaatgcgct aaatgcaatt aatccaccaa
     1441 tccagtctag tccaatatct ttaaaagcag tagccaaaaa tgcttctgta tttaggtgtg
     1501 tatagtgcac catgcctgtt aacactaagg ctacggcaat atacagtact gtcgaaatta
     1561 ataaagcata tatcatgcct cttggcaaat cacgctgtgg atttttacat tcttcagcag
     1621 ttgtcgatat cgcatcaaat ccaatataag caaaaaatac agctgacaca cctttcaaca
     1681 caccagacac tccattgggt gcaaatggcg tccaattgcc tggcactaca taaaatattc
     1741 caatggcaat gaccactaaa ataacgccta ctttaaacaa taccattccg tttgcaaaat
     1801 tctttgattc tttgatacct acatacacta aataagtaat gaaaatattg atgacaaacg
     1861 ctggaatatt aataataaaa ggtattccaa ataattttgg agctgctgcc catagttctg
     1921 gcatattgtg ttttgcttct aaaaaacttg tcgttaagta tctgggtaat tctataccga
     1981 agccatgcaa aaaattagta aaattgccac tccaagaaat cgccaccgtt acatttccaa
     2041 ttgcgtattc taagagtaaa tcccagccga ttacccaggc aattaattca ccaaaaacgg
     2101 cataagaata tgtatatgcg ctgcctgcaa tcggaattcg cgatgcaaat tctgcatagc
     2161 acaatgctga aaatccacaa gcaatagcaa tacctacaaa caataaagaa actgctggtc
     2221 caccatcaaa acatgctttt ccaatagtag aaaagatacc cgctcctact actgcagcaa
     2281 tacctaatgc cgttaaatct ttaacgccta aatcacgttt tagactttct gcatttccat
     2341 gtgattcaat caaatcagga tgctgattgg cttcttttag tatggtgtct atcggttttt
     2401 tgcggaaaag atgatgccaa ttcattgtta cgatttgcat aaagatagag aaaaaataaa
     2461 aaaaatggca accttttggt tgccaagtga ccccgctggg gctcgaaccc aggacccact
     2521 gattaagagt cagctactct accaactgag ctacgaagtc agtgttcaaa taaagataca
     2581 atttttaaaa caaaatactt aaaccacttg tatccgttaa agctttcata aaagaaacaa
     2641 tatctttttt ctcttgtgtt gtcaattgca aacgcttgtc tggcaaggtt tgatttggca
     2701 cattcaactt catgcctgcg ccaccaccat gttcataaaa ctctactact tcttctaagg
     2761 ttttaaaacc accattatgc atataaggtg ctgtaatatc tacattgcga accgtagatg
     2821 tttttatagc aaacttaaat tggtcaaaag caaaattttt aaatctacct atatcatcat
     2881 caagcacagg attaatggta tcaaaagatg tagtaatgcc taaaacttcc gcttctgaca
     2941 caccataaaa aggaggcacc aaaccaaaaa atgttggtgc aaaatgacaa gaaccgcatt
     3001 gcgcttttcc cataaataaa ttaaatccac gttttacagc tgcattaatt tcatgtattt
     3061 cgccacgcat atatttgtca aaagtggaat t
//
LOCUS       DAOQQV010000180         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_16252_length_3085_cov_7.263036, whole genome shotgun sequence.
ACCESSION   DAOQQV010000180 DAOQQV010000000
VERSION     DAOQQV010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16252_length_3085_cov_7.263036"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLL59_12140"
     CDS             complement(<1..605)
                     /locus_tag="PLL59_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24605.1"
                     /translation="MTTTSALKITIVAATKLELQPLFQQLQPKATSQNDLYIVNDNLH
                     ILITGMGMMNTAAHVAIYTQQNERDFYIDAGICGAFNRSLNIGDVFQIASETYGDFGV
                     ENDEEFQDFFDLGFIDKKEDAFKYGLCEPIISEFHKKISLPKASSITVNKVHGNEKTI
                     KVIQEKYNADTENMEGLAFYYVMKLINKPSIEIRSISNYVE"
     gene            708..1157
                     /locus_tag="PLL59_12145"
     CDS             708..1157
                     /locus_tag="PLL59_12145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMY24606.1"
                     /translation="MQSILVKHFRDLSPFELYDILQLREEVFQLEQTCLYKDIDDKDK
                     YCHHLLLYKNEILVAYCRLVPTQISYHEYVSIGRVVSKQTHRKTGYGRLLMDYAMQQM
                     DILFPQQSIKISAQQYLQKFYESYGFVKVGEWYMEDDIPHISMVFTK"
     gene            complement(1164..2096)
                     /locus_tag="PLL59_12150"
     CDS             complement(1164..2096)
                     /locus_tag="PLL59_12150"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HMY24607.1"
                     /translation="MQVHKGIHNLPNFKNAVLTIGTYDGVHFGHQQIIKRINDIATAI
                     NGESILLTFDPHPRLILNPKDTTLKLISTIDEKIELLNSFELDHLVIAEFSKDFAAMD
                     AEEYVQKILIDNFHPKKIVIGYDHHFGKNRSGNIDLLRKLANKYNYEVEEISAQTLDE
                     ISVSSTKVRNALLHGDIQQANQFLAHPFTIKGKVVHGDKIGRILGFPTANIEISYPHK
                     LIPASGVYAVKIKINQQYYKGALSIGYRETVFDNSKLTIEVFILDFDGDLYNQELDMI
                     FVQFLREQIKYENWELLKLQIEKDVADVRQAVNI"
     gene            complement(2107..2781)
                     /gene="truB"
                     /locus_tag="PLL59_12155"
     CDS             complement(2107..2781)
                     /gene="truB"
                     /locus_tag="PLL59_12155"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HMY24608.1"
                     /translation="MNNFEEGEVILIDKPLDWTSFDVVNKIKFALKKKYGNIKIGHAG
                     TLDPKASGLLIICTGKKTKTIEAIQNAEKVYTGTFYIGATTECYDTEKEPNQTFDITA
                     ITTEKLLSNASTFIGEQEQFPPAHSAVKINGKRAYELARAGKELTLKSKKITIIDLQL
                     SNIQLPLVDFYVACTKGTYIRSLANDFGKRLNNGAYLYALRRTKIGNYNVDDALSIEQ
                     FMEKIK"
     gene            complement(2792..>3085)
                     /locus_tag="PLL59_12160"
     CDS             complement(2792..>3085)
                     /locus_tag="PLL59_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMY24609.1"
                     /translation="AAEFSFFLAVPTMLAVTMYSVFLKDWTHNDIAKKGYELLFSGNN
                     LIYFLIGNIIAFIVAVLAIKLFIEIIAKYGFKPWGWYRIVAGILLLLYFNFTK"
BASE COUNT          949 a          511 c          486 g         1139 t
ORIGIN      
        1 ctttcaacat aatttgatat agaacgtatt tcaatgcttg gtttgttaat taatttcatg
       61 acataataaa aggcgaggcc ctccatattt tctgtatcag cattgtattt ttcttgtata
      121 acttttatcg ttttctcgtt gccatgtact ttatttactg taatcgagct agcttttggt
      181 aaacttattt ttttatgaaa ttctgatatt ataggctcac aaagcccata tttaaaggca
      241 tcttcttttt tgtcgataaa acctaagtca aagaaatctt gaaattcttc atcattttca
      301 acaccaaaat caccatatgt ttctgatgca atttgaaata catcaccaat gtttaaactt
      361 ctattaaatg cgccacaaat tcctgcatca atatagaaat cacgttcgtt ttgttgtgtg
      421 taaatagcaa catgtgcagc cgtgttcatc attcccattc cagtaatgag tatatgtaga
      481 ttgtcgttta cgatatacaa atcattttgc gaagtagctt tcggttgtaa ttgttgaaat
      541 aagggttgca gctccaattt tgttgcagca acaatggtga tttttaatgc tgatgttgta
      601 gtcatttcca atataattat tgtcaaagat aaggcttaaa taaaattctg tttctacaac
      661 aagcaatatt attacatttg tatgtcttat tcactattat tctttgtatg caatctattt
      721 tagtaaaaca ttttcgcgat ttatcacctt ttgagttgta cgacatatta cagttgagag
      781 aagaagtatt tcaattagaa caaacttgcc tttataaaga cattgatgat aaagataaat
      841 attgccatca tttactctta tataaaaatg agattttagt tgcctattgt cgtttagtgc
      901 caacacaaat ttcgtatcat gaatatgttt ctattggtag agttgtaagc aaacaaacac
      961 atcgtaaaac aggctatggt cgtttgttga tggactatgc aatgcagcaa atggatattt
     1021 tatttccgca gcaatctata aaaatatcag cgcagcagta cttacaaaaa ttttatgaaa
     1081 gctacggttt tgttaaagta ggcgagtggt atatggaaga tgatattcca catatttcta
     1141 tggtttttac aaaataagtt agtttaaata tttactgctt gtcgcacatc ggcaacatct
     1201 ttttcaattt gtaattttag taattcccaa ttctcatatt taatttgttc tcgaagaaat
     1261 tgaacaaaaa tcatgtcgag ttcttgattg tataaatcac catcgaaatc taaaataaaa
     1321 acttctatag tcagtttact attgtcgaat actgtttctc tgtatccaat acttaaagca
     1381 cctttgtaat attgttgatt tattttgatt tttacagcat acacacctga cgcaggaatg
     1441 agcttatgtg gataggaaat ctcaatgttt gcagtaggaa agcctaatat tcttccaatt
     1501 ttatcgccat gcacaacctt gccttttatc gtaaatgggt gcgctaaaaa ctgattggct
     1561 tgttgaatat ctccgtgcaa taatgcattg cgaacttttg tagagctgac tgaaatttca
     1621 tctaaagttt gtgcagatat ttcttccact tcgtaattgt atttattcgc caactttcta
     1681 agtaaatcaa tatttccact tctgtttttt ccaaaatgat ggtcataacc aatcactatt
     1741 tttttaggat gaaaattatc gattaatatt ttttgaacat actcttctgc gtccatagca
     1801 gcaaaatctt ttgaaaattc agcgatgact aagtggtcta gctcaaaaga atttaataat
     1861 tcaatttttt cgtcaatggt agaaattagt tttaaagtgg tgtccttagg gtttaatatt
     1921 aaacgaggat gcggatcaaa tgtgagcaaa atactttcac cattgattgc tgtagcgatg
     1981 tcgttgatgc gtttgataat ttgctgatga ccaaaatgca cgccatcata cgtgccaata
     2041 gtcagtacgg catttttaaa atttggcaaa ttatgtattc ctttgtgaac ttgcatcgac
     2101 acaatattat ttaatttttt ccataaattg ttcaatgctt agtgcatcat caacattgta
     2161 atttccaatt ttagttctgc gcaatgcgta taaataggca ccattattta agcgtttgcc
     2221 aaaatcgttt gccaaacttc tgatgtatgt acctttcgta caagccacat agaaatcaac
     2281 taatggcaat tgtatattgc ttaattgtaa atctataatg gtgatttttt tagattttaa
     2341 agtaagctct tttcctgctc ttgctaattc ataagctctt ttgccattta tttttaccgc
     2401 agaatgagca ggtggaaatt gctcttgttc gccaataaat gtgcttgcgt tggacaataa
     2461 tttttcagta gttattgcag taatatcaaa cgtttgattg ggttcttttt ctgtgtcata
     2521 gcattccgta gtagcgccaa tgtagaaggt gccagtatat actttttctg cattttgtat
     2581 tgcttcaata gtttttgtct ttttaccagt gcaaataatg agtaagccac ttgctttagg
     2641 gtcaagtgtc ccagcatgtc caattttgat gtttccgtat tttttcttta gtgcaaattt
     2701 tattttattc acaacatcaa agctcgtcca gtctaatggt ttgtctatta ggattacttc
     2761 gccttcttca aaattattca cgtgtgcaat cttattttgt gaaattaaaa tacaataaaa
     2821 gcaaaattcc agcaactatc cgataccaac cccaaggctt gaagccatat ttagcaatga
     2881 tttcgataaa taatttaata gcaagcaccg caacaataaa tgcgattata tttcctatta
     2941 gaaaataaat taggttattg ccactaaata gaagttcata tccttttttt gctatgtcat
     3001 tgtgcgtcca atcttttaag aaaacggaat acatcgttac ggcaagcata gtaggtactg
     3061 ctaagaaaaa tgaaaattca gcagc
//
LOCUS       DAOQQV010000181         3078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_16320_length_3078_cov_7.821370, whole genome shotgun sequence.
ACCESSION   DAOQQV010000181 DAOQQV010000000
VERSION     DAOQQV010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16320_length_3078_cov_7.821370"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLL59_12165"
     CDS             <1..489
                     /locus_tag="PLL59_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24610.1"
                     /translation="QKQYIDFFDNICKSTLSPKPKIIITAILLSEIVNRYLRDVGFKI
                     YCEENHLDKNNKELYKTNYRKSEQFNVDYTFICSEIKAYHNFCDLQNDLFGSDVTLKV
                     LLKNPNKGLDFNDSYYYHLSVKNNFSIVTNDSDFFVEDVEVITLNNTLYEKGKNTIVP
                     MK"
     gene            complement(687..1301)
                     /locus_tag="PLL59_12170"
     CDS             complement(687..1301)
                     /locus_tag="PLL59_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMY24611.1"
                     /translation="MRQRYILFFFALISILPSNAQSTAKDYFMSAIQREKDGDKPGAI
                     LDYTKSINLDATHYQTFNNRGILKNDLQDYKGALEDYSKAIALNPSFVDAYNNRGNAK
                     SKLKDFKGALKDYTKALELDAKNPHVYYNRGNVYFDLKKYNEAVKDFSKTVAINPEYA
                     RAFFNRANCKIMLKDKSGACSDYRKAEKLGYQKATAEIKKHCGK"
     gene            complement(1348..1770)
                     /locus_tag="PLL59_12175"
     CDS             complement(1348..1770)
                     /locus_tag="PLL59_12175"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HMY24612.1"
                     /translation="MKLKNWHDSHKEVSSFGQRLADSVASGMGSWTFIIAQTVFVLAW
                     MLLNIVGYIKHWDAYPFILLNLLFSTQAAYAAPIIMMAQNRQADRDRAQAQADYHTNL
                     EAKLEIENLQKRLDAIEIEKLDKIIALLEKNNSTNKII"
     gene            complement(1821..2330)
                     /locus_tag="PLL59_12180"
     CDS             complement(1821..2330)
                     /locus_tag="PLL59_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HMY24613.1"
                     /translation="MTRIGILSDTHNYLDEKIFDYFKNVDEIWHAGDIGTNAITDKLR
                     AFKPLRAVYGNIDGATLRTEFQEDLIFTIDNCKILMTHIAGAVGVYNQRVRDLLKKEQ
                     PTILVCGHSHIVKVMKDTKFNLLHINPGAAGIHGFHKVKTILRFEIENGKPQHMELIE
                     LGLRGKLEV"
     gene            complement(2340..>3078)
                     /gene="miaA"
                     /locus_tag="PLL59_12185"
     CDS             complement(2340..>3078)
                     /gene="miaA"
                     /locus_tag="PLL59_12185"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HMY24614.1"
                     /translation="HHFIANKSIHDYFSAGEYEREVLILLERLFLSNDVIVLCGGTGF
                     YINAILNGFDEMPKVDENIRLELNQRLLQFGIEPLQQRLKLLDEDTYNTIDIHNTQRV
                     IRALEICIGTQQPFSSFKHKKSVQRNFIPIKIGLNISKEQLHHNINKRVEAMFESGFL
                     EEAKSLFAYKQHNALQTVGYKELFDFLEHKTSLSGAVDLIKLHTRQYAKRQLTFFNKH
                     KDYTWFEPKEIKKILDFINVERNIKND"
BASE COUNT          979 a          545 c          450 g         1104 t
ORIGIN      
        1 caaaagcaat acattgattt ttttgataat atttgcaagt caacgttatc accaaagcca
       61 aaaattatta tcacagctat tttgctttca gagattgtaa atagatattt aagagatgtt
      121 ggctttaaga tttattgtga agaaaaccat ttagacaaaa acaataaaga attatacaaa
      181 actaattaca ggaaatcaga acaatttaat gttgattaca catttatctg ttctgaaata
      241 aaagcatatc ataatttctg tgacttacag aatgatttat ttggcagtga tgttacatta
      301 aaagtactat taaaaaatcc aaataaagga cttgatttta atgatagcta ttactaccat
      361 ctttctgtaa aaaataattt ttctattgta acaaatgatt ccgatttttt tgtagaagat
      421 gtcgaagtga taactttaaa taatacactt tacgaaaagg gaaaaaacac aatagttcct
      481 atgaaataat atagttaggt tttacaaaga atgaattgca tttttgccaa aaccgataac
      541 caaaccgtct gcaaatttta aaaatgagta caaatcattg attatcaata ttaattttac
      601 attaattcaa gtcctactct cacagcaata aaaagagtga aggtctcgcc tgtggcgaga
      661 ccttcactct tttatatcta taaacattat ttaccgcaat gcttctttat ttccgctgta
      721 gctttttggt agcctaattt ctctgctttt ctataatcag aacaagcacc actcttatct
      781 ttcaacataa tcttacaatt agctctatta aaaaatgcac gtgcatattc tggattgatg
      841 gctaccgttt tgctaaaatc ttttactgct tcattgtatt tttttaagtc gaagtacaca
      901 ttgcctctat tgtaatatac atgcggattt ttggcgtcta gctccaatgc ttttgtataa
      961 tctttcaatg cgcctttaaa atcttttaat ttactttttg cattgcctct attattataa
     1021 gcatctacaa aagaaggatt caatgctatt gctttagaat aatcttctag tgcacctttg
     1081 taatcttgta aatcattttt taaaattcct ctattattaa aagtctggta atgcgtagca
     1141 tctaaattta tcgacttcgt atagtctaat attgcgcctg gtttatcacc atctttttcg
     1201 cgctgtatgg cgctcataaa ataatctttc gcggtgcttt gcgcattcga cggcaatata
     1261 gaaatcaaag cgaaaaaaaa taaaatgtat cgttgtctca taaaaaatct cttacacaaa
     1321 agtagtaata atagacgctg taaaacatta aataatctta tttgtgctat tgtttttttc
     1381 taacaaagca atgattttat cgagcttttc tatttcgatg gcatctagtc ttttttgcaa
     1441 attttctatt tctaattttg cttctaaatt cgtatgataa tcagcttgcg cctgtgctct
     1501 gtctctgtct gcctgcctgt tttgcgccat catgataatg ggtgcagcgt atgcagcctg
     1561 tgtcgaaaac aatagattta acaagataaa aggataggcg tcccaatgtt ttatataacc
     1621 tacaatattc aacaacatcc aagctaatac aaatactgtt tgcgcaatta taaaagtcca
     1681 tgagcccatg ccactggcta ctgaatctgc caatcgttga ccaaaactgg aaacttcttt
     1741 gtggctatca tgccaatttt ttaatttcat aaatgccatt tatatgcatt aaatgtaatc
     1801 aataaatacc agcgcggcaa ctaaacctct aatttaccac gcaatcctaa ttctatcaat
     1861 tccatatgct gtggcttgcc attttcaatc tcgaaacgca atatcgtttt tactttatga
     1921 aaaccatgta tgcctgcagc acctggattt atatgtaata aattaaattt ggtgtctttc
     1981 atcaccttca cgatatgcga atggccacac actaatattg ttggctgttc cttttttagc
     2041 aaatcgcgta cacgttgatt atacacgcca acagcacctg caatatgtgt catcaatatt
     2101 ttacaattat caattgtaaa aattaaatct tcctgaaatt ccgttcgcaa tgttgcgcca
     2161 tcaatattgc catatacggc gcgcaatggt ttgaaagcac gcagtttatc tgttatcgca
     2221 tttgttccga tatcgccagc atgccatatt tcatcgacat ttttaaagta gtcgaagatt
     2281 ttttcgtcga gataattatg tgtatcagag agtatgccaa tgcgtgtcat attattaact
     2341 taatcgtttt taatattccg ttctacatta ataaaatcaa gtattttctt tatctctttc
     2401 ggctcaaacc aagtataatc tttatgttta ttaaagaaag taagttgtct ttttgcgtat
     2461 tgtcgcgtat gtaattttat taaatccaca gctcctgata agctcgtttt atgttccaaa
     2521 aaatcaaaca actctttata gcctactgtt tgcagcgcgt tatgttgttt ataggcaaat
     2581 aaagattttg cttcttctaa aaagccagat tcaaacatgg cttctacacg tttattgatg
     2641 ttatgatgta attgttcttt agaaatattc aatcctattt ttataggaat aaaatttctt
     2701 tgaacggact ttttgtgttt gaaagatgaa aagggttgct gtgtaccaat gcaaatttcc
     2761 aatgcacgaa tcacacgttg tgtgttgtgt atgtcgatag tattatacgt gtcttcgtct
     2821 agtaatttta atcgttgttg taatggttca ataccaaatt gcagtaatct ttgattgagt
     2881 tccaagcgta tattctcatc aacttttggc atttcgtcaa agccatttaa aatggcatta
     2941 atataaaatc cagtgccacc gcaaagcact atcacatcat ttgacaaaaa taatctttct
     3001 aataaaatta gcacttcgcg ttcgtattca ccagcagaaa aataatcgtg tatgctttta
     3061 ttggcaataa aatgatgc
//
LOCUS       DAOQQV010000182         3065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_16437_length_3065_cov_5.232558, whole genome shotgun sequence.
ACCESSION   DAOQQV010000182 DAOQQV010000000
VERSION     DAOQQV010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16437_length_3065_cov_5.232558"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2811
                     /locus_tag="PLL59_12190"
     CDS             <1..2811
                     /locus_tag="PLL59_12190"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMY24615.1"
                     /translation="TSNIYVAAPSGTNYIGASASIAVQPSKDTTTVEIFLSVGATLKG
                     KVFALGKSVSAAEIYIEGKPYIKTKTDNNGNYSIGVPTGEYTLIASKTGYIADKKSMT
                     FENKIYTQDFELKDPGFNASTILGFDLILLASTSMPTPNEFKISGQITNIPSNTLFSI
                     SKAKKIDFIDQIVIKQGDNIVPKSGTLVLVDQQLDFKLFDYLNVNLKKAGGLKVVSNN
                     GDNTKGIISGELLLDVKKTFQSKYGLTWPEGIYKLQSSNTGDFNAFYSNASAPDIATL
                     KLEAPTSGWEVFGIKLKPDFNNTSINKNGLNIAGSIELTDVPGLGNASLNINTFQIGT
                     NGDIIKLELDVDPNPELNFMSWKMILNKASINQYGLRFGGKLSVPIPSSNAANFIFSD
                     VNVTKSGLNGGSYKLLGNINIFDVASFSGITTSPFSIGKVVGTNYYKINGGGSLSFNS
                     SLLDKDISIQDFSISSDANLKFSASPNLNFDFANGMASFKLNGLNLSTSNKEISLNGG
                     FGLNIPGFGGAINSNIHYSKSNIRIDKLGLSASLGSIGSYSANVEFDTNKFLGDGKIS
                     ISGITDLSMAFEYSKLNNNKKIKADIKTNLAISIGVLTFEKIGGGFIYHSSANKYGVN
                     LTGRMTLAPGTSAAVALENIIVGVEASTAGPVLYGSAVPSILSMNVGYSEFKFDIPQK
                     SFFINTNLSKSLNIIPNVGMSASGSFMLAASAKSADTYWLIALYTKMKMLGLFDENLN
                     ITAAYNLQRASHPEFIAYTSFIPEAYLNAGKINGIHTSFKSLKGRTKDNAVCNGVSGL
                     ATLCAYAYADMNLNIHSNLSSGTFGFDVSQNWGAGGSADFVGYPVAGASVSVGFGLNG
                     AYNGNAWTINGSGSANANAHVGCAGSGCGNGVSWGCCVDPCVWSSCEVCPCPCGARIC
                     IHPSVSASYSSANGDFNVDLDW"
     gene            2829..>3065
                     /locus_tag="PLL59_12195"
     CDS             2829..>3065
                     /locus_tag="PLL59_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24616.1"
                     /translation="MKKIILLLSLFIYLASSAQKEIEIPLKQGKNGIYILLSNKGFSD
                     KNPMYGKYTKVVIKRAIDDNKFKEIGKMTAALSAN"
BASE COUNT         1053 a          436 c          575 g         1001 t
ORIGIN      
        1 acatctaata tttatgtagc agctccatca ggaacaaatt atatcggagc aagtgcttcc
       61 attgctgttc aaccaagcaa agacacgaca acagtagaga tatttttaag cgtaggtgca
      121 acattaaaag gtaaagtgtt tgctttagga aaatctgttt cagctgccga aatttatata
      181 gaaggtaaac catatatcaa aacaaaaaca gataataacg gcaactatag tattggagtt
      241 ccaacaggtg aatacacact aattgcatct aaaacaggat atattgccga taaaaaatcg
      301 atgacatttg aaaataaaat ctatacgcaa gattttgaat taaaagatcc tggatttaat
      361 gcatcaacaa ttctcggctt tgatttaatt ttattagctt ctacaagcat gccaacacct
      421 aatgagttta aaatttctgg tcaaattacg aacattcctt caaacacatt attttctatt
      481 tcaaaagcaa aaaagattga ttttattgac caaatagtca ttaagcaagg cgataatata
      541 gttccaaaat ctggtacttt agtactagtt gatcagcaac ttgattttaa attgttcgat
      601 tacttaaacg tcaatcttaa aaaagcaggt ggcttaaaag ttgtatctaa taatggagat
      661 aatacgaaag gtataatatc aggagaatta ttattagatg tcaaaaaaac gtttcaaagt
      721 aagtatggtt taacttggcc agaaggtatt tataaattac aaagctcaaa cactggtgat
      781 tttaatgctt tttatagcaa tgcttcggcg cctgatattg ctacattaaa attagaagcg
      841 cccacatcgg gatgggaagt gtttgggata aaactcaaac cagattttaa taatacttca
      901 attaataaaa atggattaaa tattgctgga agcattgaat taactgatgt tccaggcttg
      961 ggtaatgcat ctctaaatat aaatacattt caaatcggca caaatggaga tattataaaa
     1021 ttagaattag atgtagatcc aaatccagaa ttaaatttta tgtcatggaa aatgatatta
     1081 aataaagcaa gtattaatca atacggcctt cgttttggtg gtaaattgtc agttcctatt
     1141 ccaagttcaa atgcagcaaa ttttattttt tcggacgtaa atgttactaa aagtggactt
     1201 aatggcggaa gctataaatt attaggcaat ataaacatct ttgatgttgc ttctttttca
     1261 ggaattacta caagcccgtt tagtatagga aaagtagtgg gaacaaatta ttacaaaata
     1321 aatggtggtg gctcattatc ttttaattcg tctttgttag ataaagatat tagcatacaa
     1381 gatttttcta tttcttcaga tgcgaattta aaattttcag catcgccaaa tctcaatttt
     1441 gattttgcaa atggtatggc atcttttaaa cttaatggat tgaatttgtc aacttcaaat
     1501 aaagaaatta gccttaatgg tggtttcgga cttaatattc ctggatttgg tggtgctata
     1561 aacagtaata ttcactattc aaaatcaaat attcgcatcg ataaacttgg attgtcggca
     1621 tctttgggta gcattggtag ttatagtgca aatgttgaat ttgatactaa taaatttttg
     1681 ggcgatggga aaataagtat ttcaggaatt actgatttaa gtatggcatt cgaatacagt
     1741 aaattaaata ataacaaaaa gattaaagca gacataaaaa ccaacttggc tatttcaatt
     1801 ggtgtgctta cattcgaaaa aattggaggt ggttttattt atcattcatc ggcaaataaa
     1861 tatggagtta atcttacagg acgaatgaca ttggcacctg gtacttctgc tgctgtagca
     1921 ttagaaaata ttattgtagg cgttgaagcg agtactgcag gacctgtttt atatggctct
     1981 gcagtacctt ctattttgtc aatgaatgtc ggctattcag aattcaaatt cgatattcca
     2041 caaaagtcat tttttataaa tactaatttg agtaaaagct taaatataat accaaacgta
     2101 ggcatgagtg cctctggtag ttttatgttg gcagctagtg caaaatctgc tgatacttat
     2161 tggctgattg cattgtatac taaaatgaaa atgttaggat tgtttgacga gaatttgaat
     2221 attactgcag cgtataattt acaacgcgca tcacaccctg aatttattgc atatacatct
     2281 tttattcctg aagcatattt gaatgctggt aaaataaatg gaatccatac atcatttaaa
     2341 tctttaaaag gtagaactaa agacaatgct gtttgcaatg gcgtaagtgg tcttgcaacc
     2401 ctatgtgcct atgcttatgc tgatatgaat ttaaatattc atagtaattt atcatcaggc
     2461 acttttggat ttgatgtaag tcagaattgg ggtgcaggcg gtagtgcaga ctttgtcggc
     2521 tatcctgtcg ctggtgcaag tgtaagtgta ggatttggtt taaacggtgc atataatgga
     2581 aatgcatgga ctataaatgg aagcggaagt gcgaatgcga atgcacatgt aggctgtgct
     2641 ggtagtggtt gtggaaatgg cgtgtcttgg ggctgttgtg ttgacccttg tgtttggagt
     2701 tcttgcgaag tttgtccttg tccatgcgga gcaagaattt gcatccatcc ttcagtttcc
     2761 gcaagctatt cttcagctaa tggtgatttt aatgttgatt tagattggta ataagaaaaa
     2821 aataaaacat gaaaaagata attcttctgc tcagcttatt tatttattta gcttctagcg
     2881 cacagaaaga aatagaaatt ccattgaaac aaggaaaaaa tgggatttat atcttactgt
     2941 caaataaagg tttttctgat aaaaatccaa tgtatggcaa atacactaag gtagtgatta
     3001 aacgtgcaat agatgataat aaatttaaag aaattggaaa gatgacagca gccctaagtg
     3061 ctaat
//
LOCUS       DAOQQV010000183         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_16829_length_3020_cov_7.406071, whole genome shotgun sequence.
ACCESSION   DAOQQV010000183 DAOQQV010000000
VERSION     DAOQQV010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16829_length_3020_cov_7.406071"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="hemH"
                     /locus_tag="PLL59_12200"
     CDS             complement(<1..782)
                     /gene="hemH"
                     /locus_tag="PLL59_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HMY24617.1"
                     /translation="MDKKTGILLVNLGTPDTPSNSDVYKYLIEFLTDERVIDFGWWKR
                     NLLVRGAIVPARYKNSAKTYREIWDKERGSPLLYHLQDLAAKVQQQLGNDFVVEYAMR
                     YQNPSLQGALEKLQKLQVNKIVVLPLFPQYSSACNGSVLQRVQEITASWQTIPDIKLI
                     HHFYDNAKFIDAFVARGNEHNFEDFDHVLFSYHGLPARQLQKADLSKKHCLQDASCCA
                     SITEINQHCYKAQCYATTKLIVDKLGLSKDKYTVTFQSRLGK"
     gene            917..2218
                     /locus_tag="PLL59_12205"
     CDS             917..2218
                     /locus_tag="PLL59_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24618.1"
                     /translation="MPKPIKCPQCGASEANLLQNDIYQCKFCGSIYEFGDVKDDFDKS
                     IFKHKPSKQDIPSTTSTNTSSTIKQIILWAFLFFLISIGISLYFTLRVKNKPFALSSF
                     KTERSSFYDNNNESTNYFTFVTTNSGAKVWTISRVNANDLSTVNYYINELNLAEKKVE
                     KSFQLGKTISWKQSNDDAYRISFFQAIGDTCWLIYGSNLIAYDANTFTEVLNNETLKR
                     KNAVLKSGIAEIEKNHNGEGFRITTKDGYKFIYDLSNNSIVAEKQSAENTEKNEATID
                     VTEYYFTTAERQQLYRVKRKVSAINPSIFHESSLTSMLSDNSDWYRRVYKINSVEEVK
                     KGSIFFNAAVLYSTNDKIVAVYQKELGDSASIYLTCFDTNGNERWTINDMETAYIKPF
                     LQSTNAISALNGKELVMMQTYQSIICLDIDKGNIFWKFKPY"
     gene            2307..>3020
                     /locus_tag="PLL59_12210"
     CDS             2307..>3020
                     /locus_tag="PLL59_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMY24619.1"
                     /translation="MFSTHLPSISWEKIQENISQINTQDVQAILSRSNNDSNNTLQDF
                     LCLISPAAAAYLDEMAQLSQGLTQARFGKNIQLYAPMYLSNECQNICTYCGFSYTNKI
                     PRKTLTDKEILQEFDVIKSYGFESILLVTGEHDKLVGIDYLLHAVALAKQHFAQVSIE
                     VQPLEQHEYTQLKEAGVFAVLVYQETYHKETYKNYHPKGKKSNFNYRLETPDRIGNAG
                     MYKIGIGVLLGLEDWRVDSY"
BASE COUNT         1093 a          517 c          507 g          903 t
ORIGIN      
        1 tcttttccta atcgcgattg aaatgtgacc gtatatttat ctttcgagag tccaagttta
       61 tctacaatca atttcgttgt ggcatagcat tgtgctttgt agcaatgctg atttatttct
      121 gtaatagagg cacagcaaga tgcatcttgc aagcaatgtt ttttacttaa atctgctttt
      181 tgtaattggc gtgctggcaa gccatgataa gaaaataaaa catggtcaaa atcttcaaaa
      241 ttgtgttcat ttcctcgcgc cacaaatgca tcaataaact tggcattgtc atagaaatga
      301 tgaatcaatt taatatcagg aatggtttgc caagaagcgg tgatttcttg aacacgttgc
      361 aaaacagaac cattacacgc tgaagaatat tgaggaaaaa gtggtaagac tactatctta
      421 tttacttgta atttttgtaa cttttctaat gcaccttgca agctaggatt ttgatatcgc
      481 attgcatatt ccactacaaa atcattgcct aattgttgtt gcacttttgc tgctaaatct
      541 tgtaaatgat acaataatgg cgagccacgc tccttgtccc aaatttcgcg ataggtcttt
      601 gccgaatttt tataacgagc aggaacgatg gcaccacgca ccaacaaatt gcgtttccac
      661 cagccaaaat ctattacacg ttcgtctgtt aaaaattcaa tcaagtattt atacacatcg
      721 ctattagatg gcgtatcagg agtgcctaaa ttaacaagta agatgcctgt ttttttatcc
      781 atttcaaata ttcaagccac aaagttagtc caaaatcaca cattttaata attcaatagc
      841 tcatcaaaac attaacttac acacacatca tcattttagc attaagattt ttagtatctt
      901 taataaaaac catagtatgc caaaaccaat aaaatgtccg caatgtggcg ctagtgaagc
      961 caatttatta caaaatgata tctatcaatg taaattctgt ggttcaattt atgaatttgg
     1021 cgatgtaaaa gatgatttcg ataaatccat attcaaacat aaaccatcta agcaagacat
     1081 tccttcaaca acatcaacga acacatcgag tactataaaa caaattatct tatgggcttt
     1141 cttatttttt ttaattagca ttggtatcag tttgtatttc acattaagag ttaaaaacaa
     1201 accctttgca ctttcaagtt tcaaaacgga aagaagtagt ttttatgaca acaacaatga
     1261 aagtacgaat tacttcacct tcgtaacaac aaattcaggt gcgaaagtat ggacaatctc
     1321 acgtgtgaat gccaatgatc tttcaacggt taattattat atcaatgagc ttaatttagc
     1381 agaaaaaaag gtagaaaaat catttcaatt aggtaaaact atcagttgga aacaaagcaa
     1441 cgatgatgcc tatcgtattt ctttttttca agcaattggt gacacttgtt ggctcatcta
     1501 tggcagtaat ttaatcgctt atgatgcaaa cacttttaca gaagtattga acaatgaaac
     1561 acttaaaaga aaaaatgcag ttttgaaaag tggtattgct gaaatagaaa aaaatcacaa
     1621 tggcgaaggt tttaggataa cgacaaaaga tggatataag tttatttatg atttatcaaa
     1681 caacagcatt gttgcagaaa aacaatcagc agaaaatacg gagaaaaacg aagcgacgat
     1741 tgatgtgact gaatactatt ttacaacagc ggaacgacaa caattgtatc gtgtaaaaag
     1801 aaaagtatcg gctataaatc catcaatatt tcatgaaagt tcgctcacca gtatgttgtc
     1861 tgacaatagt gattggtatc gacgtgtgta taaaataaac agtgttgaag aagtaaaaaa
     1921 aggcagtata tttttcaatg ctgctgtact ttattcaact aatgataaaa ttgtagctgt
     1981 atatcaaaaa gaacttggcg atagtgcttc catttatttg acttgttttg atacgaatgg
     2041 aaatgagcgc tggacaataa acgacatgga aacagcgtac attaaaccat ttttacaatc
     2101 tacaaatgcg ataagtgcat taaacggcaa agaattagta atgatgcaaa cataccaatc
     2161 cattatctgt ttagacatcg acaaaggaaa cattttttgg aaatttaaac cgtattgata
     2221 gaaatcagaa ttaaacaaaa tcaacactga taaagtagtt taaaaatcgt aaattgcagg
     2281 cagtattata acagcaacac actgtcatgt tttcgactca tttgccatcg atttcttggg
     2341 aaaaaataca agaaaatatt tcacaaataa acacgcaaga tgtgcaagct attctatctc
     2401 gttcgaataa tgacagcaat aatacattgc aagattttct atgtttgatt tcaccagcag
     2461 cagctgccta tttagatgaa atggcacaac tcagccaagg tcttacacaa gctcgatttg
     2521 gaaaaaatat acagctttat gcaccaatgt atttgagcaa cgaatgccaa aacatttgca
     2581 cctattgtgg ctttagctac actaataaaa ttcctagaaa aacattaact gacaaagaaa
     2641 tacttcaaga gtttgacgta ataaaatcgt atggatttga gagcatttta ttagttacag
     2701 gtgaacacga caaacttgta ggcatcgatt atttactgca tgcagtagca ttagcaaaac
     2761 agcattttgc gcaagtgtca atcgaagtgc aacctttgga acaacatgaa tatacacaac
     2821 taaaggaagc tggcgtattt gccgtattag tatatcaaga aacttaccac aaagagacct
     2881 ataaaaacta ccacccaaag ggcaaaaaat cgaatttcaa ttatcgattg gaaacacctg
     2941 atagaatcgg caatgcaggc atgtataaaa ttggtattgg cgtattattg ggcttagaag
     3001 actggcgcgt ggacagctat
//
LOCUS       DAOQQV010000184         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_17315_length_2969_cov_4.477694, whole genome shotgun sequence.
ACCESSION   DAOQQV010000184 DAOQQV010000000
VERSION     DAOQQV010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17315_length_2969_cov_4.477694"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..844
                     /locus_tag="PLL59_12215"
     CDS             245..844
                     /locus_tag="PLL59_12215"
                     /inference="COORDINATES: protein motif:HMM:NF024709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HMY24620.1"
                     /translation="MRKNNFISIFILFSVIYLFSCNKKEIQATVGNTPIKEAIDTKTP
                     ADTIYSQSNPSANFGFRFHATKNGKITRLGCRMPEIGTYEVTLWDFDTKNLLANTSIN
                     ITASDDFFYSLITSVAIVATKDYVISVNNSSSAISKKYFQLFKKPTPATNLYPFTVKD
                     IVIVEPLYKSGATSACPDYNNSSDFPYLRGLSDFEFIAD"
     gene            complement(1114..>2969)
                     /locus_tag="PLL59_12220"
     CDS             complement(1114..>2969)
                     /locus_tag="PLL59_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24621.1"
                     /translation="NNAAQARSFVGYGGLIMDGGSRIRIKKMNTPNPELEGVNEPYTL
                     SSGTVEFYGSHATQQQQLRGNYDTCTSCSRPKINYYNLEINANQANLQTFASQPNSTQ
                     LGDVGNVDVNSSFLLTGTLNVNSPAVFRMDQNDFIDDGTGTSQAINIKAGAGLLYANE
                     NGIKTSGTAVTDGNIRISGTRTFSTSANYGFVSSGDMVSGNGLPANVAGLYVYKTYGI
                     NTVTLNNGGTTVNGILGLQNGKIVSSDANKLILAEVAISDIKSPANAGALQDMGYDSS
                     YVQGKMGHLSNTTSEMIFPTGSAKVYGPFALTPKNGTAQTYNGDYVSTGFGTYTLDPG
                     NSSQLDHVSLVEYWNINSSITPSPNDDAKVKLFWRRHSAVSATAADWSNLRVVHFDGT
                     DWNKEDNTASGAQVAGVSRNWGSVVSAIDCANFSPFTIGTETSNNPLPVELISFSGQC
                     NDGAIQINWSTATELNSAAFLVQRSANGFDYTTVATVPSAGNSNQMRNYAIVDSTNSN
                     SNYYRLIEIDTDGKQTIYSFILVRCSEVNGVQVYYNQPKVVVEVNSNIDKQVGFNVYE
                     ISGKLLHQENKLILRGYNRFDLQIKNKLTDGIYIIQMVDGDKLTSTKLMVH"
BASE COUNT          930 a          561 c          496 g          982 t
ORIGIN      
        1 atattaaaag cttaaaaata attgattctt atttactcat atagtaaatc aattaaagtc
       61 ttcgcgcttg actggtattg tcacaagtta ttatcgccac cataaacaac atagacatta
      121 ctttaatagt cgaacggcac aacaagaccc gagttcctat tagagtcagt aattatatac
      181 tacaaattat gtatgtcata ttgaaagcca ttcaattaac tttatagtat caaaaataaa
      241 ttttatgaga aaaaataatt tcatttcaat ctttatctta ttttctgtga tttatttatt
      301 ttcttgtaac aaaaaagaaa ttcaagctac tgtcggtaat acgccaatca aagaagctat
      361 agacacaaaa acaccagcag atacgatata ttcgcagtca aatccttctg caaattttgg
      421 atttagattt catgctacaa agaatggaaa aattactaga ttaggttgca gaatgcctga
      481 aataggcact tacgaagtta cgctttggga tttcgacact aaaaatctgt tggcaaatac
      541 gagtataaac ataacggctt cagatgattt tttctattcg ttaatcacat cagtcgcaat
      601 tgtagcaact aaagattatg taatttcagt aaataattct agtagtgcca tctctaaaaa
      661 atattttcag ttatttaaaa agccaacgcc agccacaaat ttatatccat ttacagttaa
      721 agatattgtg atagtggaac cactttacaa aagtggtgca acaagtgctt gtccagacta
      781 taacaattca tctgattttc cttatttaag aggtttgtca gattttgaat ttatcgctga
      841 ttgatgtcaa aaataaagta tatacctact actgaatttt ttccttattg cttgctgttc
      901 tcctaaacaa catacaacac aattttcatt cttagattcg atgtttaaga ttcccacttg
      961 cgtgggaatg actcttgcgt gggaatgact cttgcgtggg aatgactttt gcgtgaaaat
     1021 gactttatta aatttatcgt atgcagtcct cagtaagcta aataaaaaaa tgcgctcgaa
     1081 aggagcgcat ttgtatttta aaatagctga tagctaatgt accattaatt ttgttgaggt
     1141 caacttatca ccatctacca tttgaatgat ataaatgcca tcggtcaatt tatttttaat
     1201 ttgtaaatcg aaacgattat aaccacgtag tatcaatttg ttttcttggt gcaataattt
     1261 tcctgaaatt tcatacacat taaatccaac ttgtttatca atattagaat taacttctac
     1321 tactactttc ggttgattgt aatacacctg aacgccattt acttcgctgc atcgcaccaa
     1381 tataaacgaa tagattgttt gttttccgtc agtatcaatt tcaattagac gataataatt
     1441 gctattcgaa tttgtggaat caacaatagc atagtttctc atttgattcg aattgcctgc
     1501 agaaggaact gtggcaacag ttgtataatc aaaaccattg gcagagcgtt gtactaaaaa
     1561 tgcggcactg tttaactcag tagcggttga ccaattgatt tgtatggcac catcattaca
     1621 ttgtccgcta aatgatatta actctactgg caaaggattg tttgatgtct ccgtaccaat
     1681 tgtgaatggt gagaagttag cacagtcaat ggctgacacc acagaacccc aatttctact
     1741 cacacctgcc acttgagcac cagaggctgt attgtcttct ttgttccaat ccgtgccatc
     1801 aaaatgcacc acacgcaagt tgctccagtc agcagctgtt gcacttaccg cgctatgtct
     1861 tcgccagaat aattttactt tggcatcgtc atttggcgat ggtgtaatgg aggaattgat
     1921 attccaatat tctaccaagc ttacatggtc taattgagat gaattacctg gatcgagcgt
     1981 atatgtacca aaaccggtgc ttacataatc gccattatac gtttgtgccg tgccattttt
     2041 tggtgttaat gcaaatggac catatacctt agcacttcct gttgggaaaa tcatttctga
     2101 agtggtgttg gaaaggtgac ccatttttcc ttgtacataa ctgctatcat aacccatatc
     2161 ttgcaaagca cctgcatttg ctggagattt gatatctgat atggctactt ccgctaagat
     2221 gagtttattg gcatcagaag aaacaatttt tccattttgt aaacctaaaa ttccatttac
     2281 cgttgtaccg ccattattga gcgtcactgt atttatgcca taggttttat aaacgtataa
     2341 accagccaca tttgcaggta aaccattacc agaaaccatg tcgccactgc ttacaaaacc
     2401 ataattggca ctagtagaaa aagttctggt gccagatatt ctgatattgc catctgtaac
     2461 agcagttcct gaagttttaa taccattttc atttgcatat aataaaccag cgccagcttt
     2521 aatattaatc gcttgagaag tgccggtgcc atcatcaata aaatcatttt gatccatgcg
     2581 gaagacagca ggtgaattaa catttaaagt gcctgttagc aagaaacttg aattgacatc
     2641 tacattaccg acatcaccga gttgagtaga attgggttga gaagcaaatg tttgcaagtt
     2701 ggcttgattg gcattaatct ctaaattata ataatttatt tttggtcgcg aacacgaagt
     2761 acacgtatca tagtttcctc ttaattgttg ctgttgtgta gcatgtgagc catagaattc
     2821 taccgttcca ctacttagcg tatatggttc attcacacct tctaattctg gatttggcgt
     2881 attcattttt ttgatacgaa tacgacttcc gccgtccata attaaaccac catagcctac
     2941 aaaggaacgt gcttgtgccg cattatttc
//
LOCUS       DAOQQV010000185         2967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_17332_length_2967_cov_6.848558, whole genome shotgun sequence.
ACCESSION   DAOQQV010000185 DAOQQV010000000
VERSION     DAOQQV010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17332_length_2967_cov_6.848558"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2757)
                     /locus_tag="PLL59_12225"
     CDS             complement(<1..2757)
                     /locus_tag="PLL59_12225"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HMY24622.1"
                     /translation="MKLINNRNIFHLSHYFLDKKLFLLLLITCVMLPAFSQGNIIPFP
                     KITNDTLEHTNITPNVNISSADSIVKDSTIVEAKTIKIANNDLEDQVKYTAQDSIIYD
                     IPNKTVYLFGRANIKYQEMDMHAGFIKFNWDSSEVYAKAMYDDSLGAIKRVEFKDGSG
                     EYVANEAKFNFKTKKGKSSGLVTKELEGYLHGMQVKVIDSTTMYIKGARYTTCDLDEP
                     HFYVEIGKAKVIKDKIMVGKPADVVIEGVRTPLFLPFAILPNIKTKGTGLLMPTYGQS
                     TELGFFLNNIGYFWKINDKLALTATADVYTLGSWGLHANLAYKKIYKLTGNLSFNINQ
                     QRGGSQYERFNKNRQKPPLNFGIMWQMNIDPKKMFNSSFNINLNIVSNKTYQLLNSRD
                     PQSVLATNFSSNISYSKFWPGKPYRIALNTGLVQNTQTKQIALTLPQFSFNVTRINPF
                     QSKIAVTKRKWYENIGFSYDLNVSNQLNATDSTFFTMKTLKTMRNSITHNLPISGNFT
                     LFKYLNLNVAFNYRENWYFSYLNKSYHDFLARKNDETGKIDTFYNQAVETIKYGFKSY
                     RDFNFNMSFNTQLFGTFRFKNTKLKAIRHTFRPSVNFGFSPDFTTALWKYNKTVQVDS
                     LGNVATYSIFQNNGITPSNKQGNIGLNFSNTLEIKVYSKKDTVTHTKKITLLDNLSFG
                     MNYNMLTKRFSALNISASTHITDKLNLSMNVNMDPYSIDSLNRQTNQFYWKEKRRLFR
                     LTNLNISLSGSVRSKKGMQEQNSINQSMLETQDINNQFYPKNIYERGYYNFSIPWAIN
                     YQYSLSLSRYKFNKKDTTAITQTLALGLDVNITKKWKISVSSGFDISNKSITRTDIAI
                     VRDLHCWQMEFKWTPVGFQKGFYMTIYVTSQQFNWLKLQKQKGFFDTGIFGGLGSGGI
                     GGISN"
     gene            2771..>2967
                     /locus_tag="PLL59_12230"
     CDS             2771..>2967
                     /locus_tag="PLL59_12230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMY24623.1"
                     /translation="MSKIRIACLIFLLSIHTIAHCVSKPTGSYKIKTIVLDAGHGGHD
                     TGCHGQTGSYEKDVTLKVVLL"
BASE COUNT          907 a          517 c          487 g         1056 t
ORIGIN      
        1 attgcttatt ccgccgatgc caccactgcc aagtccgccg aaaattcccg tatcaaaaaa
       61 tcctttttgt ttttgcaatt tcagccagtt aaactgttgc gatgtaacat aaatagtcat
      121 ataaaatcct ttttgaaaac caactggtgt ccacttgaat tccatttgcc agcagtgcaa
      181 gtcacgcacg attgcaatat ctgttcttgt aatgctttta ttgctaatat caaatcctga
      241 agaaacgctg attttccatt ttttggtgat gtttacatct aatcctaagg caagtgtttg
      301 tgtaattgct gtggtatctt ttttattaaa tttatatcga gacaaactca aactatattg
      361 ataattgatt gcccatggaa ttgaaaaatt atagtagcca cgttcataaa tattttttgg
      421 ataaaattga ttgttgatgt cctgtgtttc cagcatggat tggttaatac tattttgctc
      481 ttgcattcct tttttagaac gcacacttcc gctaagtgaa atattcaaat tcgtcaatcg
      541 gaataaacgt cgtttttctt tccaataaaa ttgattcgtt tgcctgttta aactatcaat
      601 agaatatgga tccatgttga cattcatcga caagttaagt ttgtctgtaa tatgcgtgct
      661 tgcactaata ttcaatgcgc tgaaacgttt cgtaagcata ttataattca taccaaaaga
      721 aagattatcg agcagcgtaa tttttttagt atgcgtcacc gtatcttttt tcgaatacac
      781 tttgatttct aaagtattag aaaaattcag acctatattt ccttgtttgt ttgatggtgt
      841 aatgccatta ttttgaaata tagaatacgt tgctacattt cctaaactat ccacttgaac
      901 cgttttgtta tatttccaaa gagcagtagt aaaatcagga ctgaaaccaa agtttacact
      961 cggtctaaac gtatgacgaa tcgcctttag ttttgtattt ttaaatctga aagtaccaaa
     1021 taattgtgta ttaaagctca tattgaaatt gaaatctcta tatgatttaa atccatactt
     1081 gattgtttct accgcttgat tgtaaaaagt atcgatttta ccagtttcgt catttttgcg
     1141 agctaaaaaa tcgtgatacg atttattcaa atagctgaaa taccaatttt cgcgataatt
     1201 aaaagcaaca tttaggttca aatatttgaa taaagtaaaa ttaccagaaa tcggcaaatt
     1261 atgcgtaatg ctattgcgca ttgttttcaa tgttttcatg gtaaaaaaag tagaatctgt
     1321 tgcattcaac tgatttgaaa cgtttaagtc ataagaaaaa ccaatatttt cataccattt
     1381 acgttttgtc accgctattt tgctttggaa tggattaatg cgtgtgacat taaatgagaa
     1441 ttgaggcaag gtcaatgcga tttgtttggt ttgtgtattt tgcaccaaac ctgtgtttaa
     1501 tgcaattcga tacggctttc ctggccaaaa tttgctataa gaaatattag atgaaaaatt
     1561 ggtagccaaa acactttgcg gatctcgaga gttgagcaat tgatacgttt tattacttac
     1621 tatattcaag ttgatattaa aactgctgtt gaacatcttt ttcgggtcga tattcatttg
     1681 ccacataatt ccaaaattca aaggtggttt ttgcctgttt ttattaaatc gttcatattg
     1741 cgaaccacca cgttgctggt tgatattaaa gctcaaattt cctgtaagct tgtatatttt
     1801 tttataggct aaattggcat gcaagcccca actacccagc gtataaacat ctgcagtggc
     1861 tgtgagagcc agcttgtcat ttattttcca gaaataacca atattattta agaaaaaacc
     1921 gagctctgta ctttgtccat aagttggcat taacaagcct gtccctttcg ttttgatatt
     1981 aggcaaaata gcaaatggca aaaacaatgg cgtacgcaca ccttcgataa caacgtcagc
     2041 aggtttgccg accataatct tgtcttttat cacttttgct tttccaattt ccacataaaa
     2101 atgtggctca tctaaatcac atgttgtata gcgtgcacct ttgatataca tggttgtgct
     2161 atcgattact tttacttgca taccatgtaa gtaaccttcg agctcttttg tcactaagcc
     2221 actactcttt ccttttttag ttttgaaatt aaacttcgct tcattggcaa catattcacc
     2281 actgccatct ttaaattcta cgcgctttat ggcgccgagg ctatcgtcat acatagcttt
     2341 ggcgtacact tcactgctgt cccaattaaa tttaataaag ccagcatgca tatccatttc
     2401 ttgatatttt atattggctc tcccgaagag ataaaccgtt ttatttggaa tgtcataaat
     2461 aatagaatcc tgcgctgtat attttacttg gtcttctaaa tcattattag cgattttaat
     2521 cgtctttgct tccacaattg tggaatcttt aacaattgaa tcagccgaag aaatgtttac
     2581 atttggcgtg atgtttgtat gttccaatgt atcgttcgtt attttaggaa atggaattat
     2641 atttccttga gaaaaagcag gaagcatcac gcaggtgatc agcagtaaaa gaaatagctt
     2701 tttatctaaa aaataatgcg acaagtggaa aatatttcta ttgttaatga gtttcaaatg
     2761 tatgtttaaa atgtcaaaaa tacggattgc ttgcttaata tttttattat ctatacacac
     2821 aattgcacat tgcgtatcaa aacctactgg cagttataaa attaaaacaa ttgttttaga
     2881 cgctggacac ggaggacatg acactggctg tcatggtcaa acaggctcgt atgaaaaaga
     2941 tgttacatta aaagtcgtgt tgttatt
//
LOCUS       DAOQQV010000186         2936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_17624_length_2936_cov_7.577230, whole genome shotgun sequence.
ACCESSION   DAOQQV010000186 DAOQQV010000000
VERSION     DAOQQV010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17624_length_2936_cov_7.577230"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLL59_12235"
     CDS             complement(<1..268)
                     /locus_tag="PLL59_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMY24624.1"
                     /translation="MSTIADEIKRRKTFAIISHPDAGKTTLTEKFLLYGGAIQTAGAV
                     KSNKIKKSATSDFMEIEKQRGISVATSVMTFEYQNTLINLLDTPG"
     gene            441..>2936
                     /locus_tag="PLL59_12240"
     CDS             441..>2936
                     /locus_tag="PLL59_12240"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMY24625.1"
                     /translation="MSLKNVSTLYFVISLFCYAINANATHIVGGVMNYQYLGNDRYEI
                     SLYVYRDCINGVPPLDDPAIIRIWDGVAESYSFRNIPFDSILDPGVPDACAQIETPVC
                     VNWTRYRDTLILPPNDFGYTIYYQRCCRNNTIQNLTYNSRGNAAMDWGATYTINIPTS
                     DNNGQNINNTSPYFLNYPPVYICVNKPITYNNSAIDSDGDSLVYSLCTPYSGAFPDDP
                     LNYYIEEVPPFEHVVWDSSYSVNDMLGGVPLSINSSTGILTGTPNTIGQFVVGVCVDE
                     YRNGQWLTQTSRDFQFNIIDCNLQVVSSFFAPTIQCNDFTVNFQNQSSGATDYIWYFG
                     DGDSSTAINPSHTYSDTGKYIVTLISKNNITNTCTATYTQEISVQYKKIDADFIVTMN
                     SCLGINDPIQFIDKSTDTLHVVAWKWHFSNGDSSNSQHPILLYNGIDTSLTITLTATS
                     VNGCSSTITKSISLFPKPAYNLNRILTKCTNTGDAQISLIAPNNSIISWSPTIGLNNP
                     FIANPTTNINTNITYYVSIKTPLENGDTCMQYDSIQVKTINSVQINTRDTLRVCKDSV
                     RLQVPISDGQSVIWSTNANFQPIIGTTASISVVQTNLSQQYFVKIIAQECEAIDSILV
                     FFSDTIPIISLADKLLQCSNNVHLNAAVDNSHQIVWSNSPSFTNILSTTNTYSTVQTS
                     KTVNYYVQASYNTCKSIDSIQVTVQDTLPLISLEDSVIICGENMAIINATVRRFTAVE
                     WSDKPDFSSIIWNSPTLYISLSETRKTYYIKAYYRDCFTIDSIIVNYNDKQLLLQLDD
                     SIQSCSNSINIQVQVQNADNIIWSTSPTFLPIIS"
BASE COUNT          958 a          514 c          491 g          973 t
ORIGIN      
        1 gaccaggtgt gtctaataaa ttgattaatg tattttgata ttcgaatgtc attacggaag
       61 ttgcaacaga aatacctctt tgcttttcta tttccataaa atcggaagtt gccgattttt
      121 ttattttatt agacttaacg gctcctgctg tttgaatagc tccaccgtac aatagaaatt
      181 tttctgtaag tgtagtttta ccagcatctg gatgtgatat aatagcaaat gtttttctgc
      241 gctttatttc gtctgcaatt gttgacatgg cacaaaaata cgaccttaat attgtatgta
      301 ataagccgct gtaagattta attactaatt aatggagaat tacttactga taaatgtatt
      361 tatactacaa ttgtagaaga tgaatctatt ttttgcttta attcaatgta ttatgtaatt
      421 tcattaacga ttacagaacc atgtctttaa aaaacgtatc tacactttat tttgttatta
      481 gtttattttg ctatgcaatt aatgcaaatg ccacacatat tgttggtggt gtaatgaatt
      541 atcaatacct aggaaatgac cgctacgaaa tatctttata tgtgtatcgc gattgcataa
      601 atggtgtacc gccattagat gacccagcta tcatccgaat ttgggacggc gtcgcggagt
      661 catatagttt tcggaatata ccgtttgaca gcattttaga tcctggtgtt cctgatgctt
      721 gtgcacaaat tgagacgcct gtttgtgtga actggactag gtatcgagac acgcttatat
      781 taccgccaaa cgactttggt tataccattt actaccaaag atgttgcaga aataatacga
      841 ttcaaaattt gacatacaat tcgagaggca atgcagcgat ggattggggc gccacttata
      901 ctattaatat tccgacttct gacaataacg gacaaaatat caacaacaca agtccctatt
      961 ttcttaatta tccgcctgta tatatctgtg taaacaagcc aattacctac aacaatagcg
     1021 ctatcgatag tgatggtgat tctttggtgt atagcttgtg tacgccatat tcaggcgcat
     1081 ttcctgacga tccattgaat tattacatcg aagaagtgcc gccatttgaa cacgtcgttt
     1141 gggattcttc atatagtgtt aatgatatgt taggcggtgt gcctttatca atcaattcgt
     1201 caacaggcat ccttactggg acgccaaata ctattgggca atttgtcgtt ggtgtatgtg
     1261 tagatgaata tagaaatggt cagtggttga cgcaaacatc acgagatttc cagtttaata
     1321 ttattgattg taacttacaa gtggtcagta gcttttttgc acctaccatt cagtgtaatg
     1381 attttacagt taattttcaa aatcaaagta gtggcgccac agattatatt tggtattttg
     1441 gagatggcga ttcttcaaca gcaattaatc catcacatac atacagcgat acaggaaaat
     1501 atatcgttac acttattagc aaaaacaata tcaccaatac ttgcacggca acttacacgc
     1561 aagaaatttc tgtacaatac aaaaaaatag atgctgattt tattgtaact atgaatagtt
     1621 gtttaggcat taatgatccc attcaattca ttgacaaaag tactgacaca ttacatgttg
     1681 ttgcatggaa atggcatttt tcaaatggcg attcttccaa tagtcaacat ccaattttat
     1741 tatataatgg aatcgacact tcgcttacaa taacgcttac tgctacatca gtaaatggct
     1801 gtagttctac gattacaaaa agtatttcac tttttccaaa gccagcatat aacttaaatc
     1861 ggatactcac aaaatgtacg aatacaggcg atgcgcaaat ttcattaatc gcgccaaata
     1921 attccattat ttcatggtct ccaactattg gtttgaataa tccttttatt gcaaatccaa
     1981 cgacaaatat caatacgaat attacttatt atgtaagcat aaaaacgcca ttagaaaatg
     2041 gagacacttg tatgcaatac gatagcattc aagttaaaac tattaattct gttcaaataa
     2101 acacaaggga taccctacgt gtttgtaaag actctgtaag gcttcaggtt cctatttcag
     2161 atggccaatc tgtgatttgg tcaacaaatg caaactttca gcctattatt ggcactacgg
     2221 caagtataag tgttgtacaa acaaatttat cgcagcagta ttttgtgaaa ataatagcgc
     2281 aagaatgtga agcaattgac agcattcttg tattttttag cgatacgata cctataattt
     2341 ctttagctga taaattatta caatgtagca ataacgtgca tttaaatgca gccgttgaca
     2401 atagccatca gatagtgtgg tcgaattcgc cttcatttac aaatattctt tctacaacaa
     2461 atacttactc aaccgtacaa acatcaaaga ctgtaaatta ttatgttcaa gccagctaca
     2521 atacatgtaa gtctattgat agtattcaag tgacggtgca agatacttta cccttgattt
     2581 cactcgaaga ttccgtaatt atttgtggtg aaaatatggc aattatcaat gccaccgtta
     2641 gacgatttac tgcggtggaa tggtcagata agcctgattt ttcaagtatt atatggaatt
     2701 ctcctacttt atatatatca cttagtgaaa caagaaaaac atattacatc aaagcttatt
     2761 atagagattg ctttacaata gatagtatta ttgtaaatta taatgacaaa caactcttat
     2821 tgcaattaga cgatagcatt caaagttgca gtaatagcat taacatacaa gtgcaagtac
     2881 aaaatgctga taatataatt tggtcaactt caccaacttt cttgccaata atttct
//
LOCUS       DAOQQV010000187         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_17641_length_2934_cov_6.862105, whole genome shotgun sequence.
ACCESSION   DAOQQV010000187 DAOQQV010000000
VERSION     DAOQQV010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17641_length_2934_cov_6.862105"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1837)
                     /locus_tag="PLL59_12245"
     CDS             complement(<1..1837)
                     /locus_tag="PLL59_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24626.1"
                     /translation="MAFAQLKVAFTPRFSDGVYGDFLTIGNNMLSTTATGNYTGASGN
                     HDLTTVFVDIDADATTFNSSSADLNGPIAGTNCVAIKKAYLYWAAADFEEENSGDEPN
                     WNYNDIKLMLPGAATYTTITADNVIYRGRTEHFYNDPYACFKDITAQVQALASPFGKY
                     QVANVRTKKGTLNPGHASSNIGTSGGWQIIFIYEGTELDQQGNSVLPQKYISVFDGYA
                     NVSSTQNDYDITISGFTTPPAGPVQTKIMFGSLEGDRDLTGDHFKIKNVSNTFIDLTT
                     GSNRPTNNFFNSRITVENSDFLSRNPASTNTLGYDIGYFTLNNPSNSVIGNNQTSAVF
                     RLTSDQEVYGLYMLGFSTDVWAPDLSPLVQTSSPATVNPGDVISYSFVVKNNGNDNAQ
                     NVVITRTIPQEVDLVEPITPLPTGVTYTYNTVTRVLSFFVANNLVEVGDAPITISYKT
                     KVKDQCYFLETACVPNTSAQLLATYKGAINPNTQTTLSSNGTTGCGLGNDKPNTTNIN
                     TPSAATWSTLPGALNRTVNCNDAQALASAQALAPVPSKCNFTLNKVSGPFVPSTNACP
                     IKGTYTNTWTFTDKCGRTSPVYTQIITVQDTTKPVITGTPADITYA"
     gene            complement(1933..2430)
                     /locus_tag="PLL59_12250"
     CDS             complement(1933..2430)
                     /locus_tag="PLL59_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24627.1"
                     /translation="MKNFVIANSILTLLFLCIVSVSNAQSGSQKQLSNLDFPLELSIK
                     KNSETALSLPSREYVLQISNTSNYPVEFLINASNISCIANQKLQQLVFEYELNQVGKI
                     IGSYILTLPPHSNLEFNLIIKNTSFTQLDSKNCTEVMAVNKNNEVLSNSISIETYIPD
                     PSQFR"
BASE COUNT          949 a          453 c          512 g         1020 t
ORIGIN      
        1 atgcatacgt aatatcggct ggcgtgcctg tgatgacagg ttttgtcgtg tcttgtacag
       61 taattatttg agtatatact ggacttgttc taccacattt atctgtgaaa gtccaagtat
      121 ttgtgtaggt tcctttaatt ggacaagcat tcgtagatgg aacaaaagga cctgatactt
      181 tattaagtgt gaaattacat ttgcttggca ctggtgccaa tgcttgagct gaagctaagg
      241 cttgcgcatc attacaatta acagttctat ttagtgcgcc aggtaatgta ctccatgtag
      301 cagcacttgg tgtattaata tttgtagtat taggtttgtc gtttccaagt ccacagcctg
      361 ttgtgccatt agagcttaaa gttgtttgtg tattcggatt aattgctcct ttgtaggtag
      421 ccaataattg agcggaagta ttaggtacgc aagctgtttc taaaaaatag cattgatctt
      481 taactttagt tttataagaa atagttattg gtgcatctcc aacctcgact aaattatttg
      541 ccacaaagaa tgacaatacc ctagttactg tattatatgt atatgttacg ccagtaggca
      601 aaggtgtaat tggttctacc aaatcaactt cctgtggtat tgttcttgtg atgactacat
      661 tttgggcatt gtcattacca ttatttttaa caacgaatga ataagaaatt acgtcgcctg
      721 gatttactgt tgctggcgat gaagtttgta ctaatggaga caagtcaggt gcccaaacat
      781 cggtagaaaa tcccagcata taaagaccat aaacttcttg atcagatgta agtcggaaaa
      841 ctgctgaagt ttgattattg ccaataacag aatttgatgg attatttaag gtaaaataac
      901 cgatatcata accaagtgta tttgtacttg ctggatttct gcttaagaaa tctgaatttt
      961 caacagtaat tctactatta aaaaaattgt ttgtaggtct atttgaacct gttgttaagt
     1021 caataaatgt attagataca tttttaattt taaaatggtc gcctgttaaa tctctatctc
     1081 cttctagaga gccaaacata atttttgttt gtactggtcc agcaggaggc gtagtaaatc
     1141 cagaaattgt aatatcgtag tcattttgtg tactggagac atttgcatag ccatcaaata
     1201 cagaaatata tttttgtgga agaacagaat ttccttgttg atccaattct gtaccttcat
     1261 agataaatat gatttgccaa ccgcctgagg ttccaatatt actactagca tgtcctggat
     1321 ttagcgttcc tttttttgtt cttacatttg ctacttgata ttttccaaat ggacttgcaa
     1381 gtgcttgcac ttgagcagta atatctttga aacatgcgta aggatcatta taaaaatgct
     1441 ctgttcttcc acgatatatt acattatcgg cagtaatcgt agtatatgtt gccgctccag
     1501 gcagcattag ttttatatca ttataattcc aattaggttc atcccctgaa ttttcttctt
     1561 caaaatctgc agctgcccaa tataaatagg cttttttgat cgcgacacaa tttgttcctg
     1621 caattgggcc atttaaatca gcactacttg aattaaacgt tgttgcatca gcatcaatat
     1681 ctacgaatac agtagtcaaa tcatgattac ctgaagcgcc agtataattc ccagtagcag
     1741 tggtcgataa catgttattt cctatcgtca aaaaatcacc atatacacca tcggaaaaac
     1801 gaggcgtgaa tgctactttc aattgagcga atgccatgtt gtatatgaga aatgttgcta
     1861 atattagcaa gcatgttctt attatttttg gatttgtcat aaaagtgaat tatgcagtgt
     1921 ttactaatat tattatctaa attgacttgg atctggaatg tatgtttcaa tagaaatact
     1981 attggacagc acttcattgt ttttatttac agccatgact tcagtgcagt tttttgaatc
     2041 taattgtgta aatgatgtgt ttttaataat cagattaaat tccaaatttg aatgaggtgg
     2101 caatgttaaa atgtaagaac caataatttt accaacttga tttaattcat attcgaatac
     2161 gagctgctgt aatttttgat ttgcgatgca actaatattt gaagcattga ttaaaaattc
     2221 aactggataa tttgatgtgt tagaaatctg aagaacgtat tctctagatg gcaaggacaa
     2281 tgccgtttca ctattctttt taatacttaa ttctaatgga aagtctaaat tgctcaactg
     2341 tttttgcgaa ccagattgtg catttgatac tgaaacaata cataaaaaca gcaaggtcag
     2401 tattgaattg gctattacaa aattcttcat attgtatggt tttgatgcag tttatacgct
     2461 gcaattatta atcaaaaaaa tgagggaatt tgtaactaaa cgataaagtc aaaaatctat
     2521 tagtattggt aaaccaatga catctatatt tgctattcag caaaatttgt aaaagttggg
     2581 aggcaagatg gtagaatgaa taacaataag tacttacaat aaatacccat tattatttat
     2641 caaattcaat cataccaaat tcggatagtc gataacgtaa catttttcat agtttgtttt
     2701 tttatggtta tgtatgtaat gtatggtgca ttacatcttt ttaataatat acaatttgta
     2761 gggctaattt cctctagata aagagtcgaa aaaaaattga tttacatcaa ttacagcaca
     2821 attttaaata taaaaaatga aatgcgcaat agctgaacga aaaaaaaata aaaaaattaa
     2881 tcactactgt ccgataaaaa taaaaaaaag agagccataa tttttggatt atgg
//
LOCUS       DAOQQV010000188         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_18013_length_2897_cov_7.089374, whole genome shotgun sequence.
ACCESSION   DAOQQV010000188 DAOQQV010000000
VERSION     DAOQQV010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18013_length_2897_cov_7.089374"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLL59_12255"
     CDS             complement(<1..435)
                     /locus_tag="PLL59_12255"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628168.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HMY24628.1"
                     /translation="MKSKEEIVQNWLPRYTGKNLEDFGEYILLTNFSKYVHLFAEWNN
                     VIVEGEDKPMLNATAHNITIINFGMGSALAATMMDLLSAIKPKAALFLGKCGGLKKKN
                     ELGDFILPIAAIRGDGTSNDYFPAEVPALPSFALQKAISTTIR"
     gene            complement(439..819)
                     /locus_tag="PLL59_12260"
     CDS             complement(439..819)
                     /locus_tag="PLL59_12260"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMY24629.1"
                     /translation="MKTLIYVVMLMLSIQAFAKSDGVLRPQFPGGEKALAQYLDTHLK
                     YPEEAQKQKYQGQSLVAFTVNEDGSLTNVRILKSSHLVLDTEALRIIKNMPKWSPAQN
                     NGAPKKEMVVLPITFDLNKKNIQY"
     gene            complement(1078..2487)
                     /locus_tag="PLL59_12265"
     CDS             complement(1078..2487)
                     /locus_tag="PLL59_12265"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMY24630.1"
                     /translation="MSEQVKFFQNVATYVDQAAGFTNFPKGLIEQIKVCNAVYQINFP
                     VKIKDKIQVIEAYRVQHSHHRLPTKGGIRYSDMVNQDEVMALAALMTYKCAVVNVPFG
                     GAKGGIKINPKEYTEDELERITRRYTFELIKKGFIGPALDVPAPDYGTGEREMSWIAD
                     TFMQFNQQELNAQACVTGKPIGQGGVHGRTEATGRGIFYGLKQACSYADDMKKIGLET
                     GIEGKTIIIQGFGNVGYHAARVLQENGAIIVGVAEYEGGLYNAKGIDVDDLKKFQIEN
                     KTIRNYPKAKFIKQSNLLMEEVCDILIPAALENQITKDNAAKIKAKIIGEGANGPITP
                     NGEAILSKKGVMVVPDMYLNAGGVTVSYFEWLKNLSHVSFGRLDHRHQDDSFNKIIDL
                     IEGNTGRRISNREKDALRGADELDIVRSGLEDTMINSYENIRTILKRHKKMNSLRTAA
                     YVDALDKIGVTYLKLGVFP"
     gene            complement(2492..2704)
                     /locus_tag="PLL59_12270"
     CDS             complement(2492..2704)
                     /locus_tag="PLL59_12270"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HMY24631.1"
                     /translation="MSKKNAILILLFVSVSKMLNAQTANVADQLVENSKLNAVIVVAA
                     IILLGIFIFLFSLEKKIKKLEQDVKN"
BASE COUNT          891 a          556 c          441 g         1009 t
ORIGIN      
        1 acgaatagta gtagaaatag ccttttgtag cgcaaaagaa ggtaatgcag gcacttccgc
       61 agggaaataa tcattagatg ttccatctcc acgaatagct gcaataggca atataaaatc
      121 tcctaattca ttcttctttt ttaatccacc acattttcct aaaaacaaag ccgctttagg
      181 tttaatggct gacaataaat ccatcattgt tgccgctaat gcgctgccca ttccaaaatt
      241 gataatggta atattatgag cagtagcatt caacatgggt ttgtcttcgc cttctactat
      301 tacattattc cactcagcaa ataaatgcac atatttagaa aaatttgtca gcaagatata
      361 ctctccaaag tcttctaaat tttttcctgt atagcgtggc aaccaatttt gaacaatctc
      421 ctcttttgat ttcatgtatt agtattggat attttttttg ttcaaatcga atgtaatagg
      481 caagacaacc atttcttttt taggtgcacc attgttttga gctggcgacc attttggcat
      541 attttttata atgcgtaatg cttcagtgtc cagcaccaaa tgtgatgact tgagaatacg
      601 tacattcgtt aaagaaccat cttcatttac tgtaaatgca acgagcgatt gtccttggta
      661 tttctgtttt tgtgcttctt caggatattt taaatgtgta tctaaatact gtgctaatgc
      721 tttttcgcca ccaggaaatt gcggtcgtaa aacaccgtca gattttgcaa atgcttgtat
      781 gcttaacata agcatcacta cataaattaa tgttttcatt gctaataatt tgattaaacc
      841 attgctgatt acacagcatt aagcatatca aatttaaaag aaagcactaa agtattttgt
      901 gttaattact aataaatgtg gataagtctg ataactcaaa atattgaata gcaaatattt
      961 accacatcaa aataaagcaa aagatgaata catttttatt cctgcaagat ttaagtttta
     1021 aaaaccattt atctaatcaa gataaaacta taccatgagt aaaaaacaga ctgactatta
     1081 tggaaatacg cctaatttca aataagtaac acctatttta tccaatgcat ctacataagc
     1141 tgccgtgcgc aagctattca tctttttatg tcgttttaat atcgttctga tattttcata
     1201 cgaattaatc atagtatctt ctaagcctga gcgcacaata tctaattcat ctgcgccgcg
     1261 caatgcatct ttttctctgt ttgaaattct acgacctgta ttaccttcaa ttaaatcgat
     1321 aattttattg aaagagtcat cttggtgtct atggtctaaa cgaccaaaac taacatgtga
     1381 taaatttttt agccattcaa aataagaaac ggtaacaccg ccagcattca aatacatatc
     1441 aggcaccacc attacacctt ttttagataa aatagcttct ccatttggtg taatcggacc
     1501 atttgcacct tcgccaatta tttttgcttt tattttagct gcattgtctt tggtaatctg
     1561 attttctaat gccgcaggaa ttaaaatatc gcacacttct tccattagca agttagattg
     1621 tttaataaat tttgcctttg gataattgcg tatcgtttta ttttcgattt ggaatttttt
     1681 caagtcatcg acatcaatac ctttagcatt gtacaagcca ccttcgtact ctgcaacgcc
     1741 tacgattatt gcaccatttt cttgcaaaac acgtgctgca tgataaccaa catttccgaa
     1801 accttgaatg attattgttt tgccttcgat gcctgtttct aatccaattt ttttcatatc
     1861 atcagcatag gaacaagctt gttttagacc ataaaaaatg cctcttcccg ttgcttccgt
     1921 tctgccatgc acaccgcctt gcccaatcgg tttgccagta acacaagcct gtgcattgag
     1981 ttcttgttga ttgaattgca taaaggtatc agcaatccaa ctcatttcgc gctcgcccgt
     2041 gccatagtct ggtgccggaa cgtctaaagc gggtccgata aatccttttt ttatcaattc
     2101 aaaagtatag cgacgagtga tacgttcgag ttcatcttct gtatattctt ttggattgat
     2161 tttgatgcca cctttagcgc caccaaatgg cacattcacc acggcacatt tgtaagtcat
     2221 tagtgcggcg agtgccatca cttcatcttg gttaaccata tcgctatatc gaataccgcc
     2281 ttttgttggc aatcgatggt gagagtgctg cacgcgataa gcttctatta cttgaatttt
     2341 gtctttaatt tttacaggga aattaatctg gtaaacagca ttgcaaactt tgatttgctc
     2401 gattaatcct ttaggaaaat tagtgaaacc tgcagcctga tctacataag tggctacgtt
     2461 ctgaaaaaat ttaacttgtt cgctcataat tttaattttt tacatcttgt tcgagcttct
     2521 ttatcttttt ctctaaagag aaaaggaaaa tgaaaatgcc caacaagatg atagcagcga
     2581 ccacaataac agcatttagc ttgctatttt caactaattg gtctgctaca ttcgctgttt
     2641 gtgcatttaa cattttagaa acactcacga aaagtaaaat taaaattgca ttttttttag
     2701 acataatgta aaaatatttt ttatctaaga ataattcgtt gatttagagt aataaaatat
     2761 aagtagaaaa ctgactattt acactccatt tttcaaattt cataagatat caagttgatg
     2821 tttttttcga tatacttttt caattctgat aattaatgtg gtaatccaaa gtgataacaa
     2881 tatccaacct attacgg
//
LOCUS       DAOQQV010000189         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_18101_length_2889_cov_5.032463, whole genome shotgun sequence.
ACCESSION   DAOQQV010000189 DAOQQV010000000
VERSION     DAOQQV010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18101_length_2889_cov_5.032463"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLL59_12275"
     CDS             complement(<1..255)
                     /locus_tag="PLL59_12275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HMY24632.1"
                     /translation="MSNNQAYPFFTIVMPTYNRANLIIDSIKTALAQDYPHFEIIVVD
                     DGSTDNTEEVVLAFNNPKIKYHKKINEERAAARNRGTQLAS"
     gene            complement(245..1363)
                     /locus_tag="PLL59_12280"
     CDS             complement(245..1363)
                     /locus_tag="PLL59_12280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMY24633.1"
                     /translation="MQRKITYILFGIDKAPAFEWIIDNIDAQRFQLEFILISGNQHTH
                     IESHCVQQKITYHVISYRSKKDIPRCVFQVYSVLRKTKPDAVHAHIFEGGLIGITAAF
                     LARVPKRIYTRHYSSYHHQFHPNGIKYDRIINKLSTHIIAICNNVKNILVQWENVPEA
                     KITLIRHGFDLNDFYHVDEARIAAVQHKYNPKKLAPIIGVVSRFTYWKGIQYTIPAFK
                     TILQKYPNAHLVLANANGDYKKEILALLSELPETSYTTIKFENDNAALFKLFDVFVHV
                     PIDQHVEAFGQIYVEALATSTPSVFTLSGVSPEFITNEENALVVDFKNSQEIANAVLR
                     ILDDDSLRQHLKQNAKNQLDNYFSLNLMIQSLEQLYEQ"
     gene            complement(1372..2484)
                     /locus_tag="PLL59_12285"
     CDS             complement(1372..2484)
                     /locus_tag="PLL59_12285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMY24634.1"
                     /translation="MKKINLTYILCSIGGYHQYIWIADTIDKNRFNLSFIFLDHTTDT
                     LKSILQQKGFVCYQLQIISKKDFFKGIFQVRKIFKKEKTTIVHTHLVNASLVGLAAAK
                     LAGVKKRIHTRHHANFHHDHFKKGVYLDNVINYLSTNIIVISKNVENIVCTKEKFHDH
                     KISLIYHGIQLAEFEVANTNKIQLMKEKYKLNGFPIIGMVSRYEEGKGVQYAIEAFKQ
                     IITEHKQAILVIANAYGPEKARISKLLQQVPADNYIEIEYEKDIKTLYKCFDIFVHVP
                     TDKEYEAFGQVYIEAMAAAVPVVCTLSGIACEYVNSNNASVVDYKNADDIARACNRFL
                     KDDIYKQNIVLTAKNDVAKIFGFEKMMSELNALYLS"
     gene            complement(2481..>2889)
                     /locus_tag="PLL59_12290"
     CDS             complement(2481..>2889)
                     /locus_tag="PLL59_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24635.1"
                     /translation="KSLFMSIGKYNENIIFQGEDIELSKKLNEANIPIFLYTPITCFH
                     NQKDRLDINEFLDRDRRGYVSQFENKKNETKHSRLKQIIYTCFIPFNFLFKKLFDILP
                     NRAAFDIITFKTIGILSSIVYFKAWYQSSKNHK"
BASE COUNT          893 a          468 c          488 g         1040 t
ORIGIN      
        1 agaagcaagc tgcgtacctc tgtttctggc agcagcacgc tcttcattta tttttttatg
       61 atactttatc ttcggattat taaaagccag tactacttct tcggtattat ctgtgcttcc
      121 atcgtcaact acaataattt cgaaatgcgg ataatcttga gcaagtgctg tttttataga
      181 gtcaataatt aaattagctc tattgtatgt gggcataacg atggtgaaaa aagggtacgc
      241 ctgattattg ctcatatagc tgttctaaac tttgaatcat caaatttaag gaaaaataat
      301 tatctaattg attttttgca ttttgtttca aatgttgacg taagctatca tcatctaata
      361 tgcgcaatac tgcatttgca atttcttgcg aatttttaaa atcgaccact aatgcatttt
      421 cctcgtttgt tatgaactct ggtgaaacac ctgaaagcgt aaaaacagac ggtgtgcttg
      481 ttgccaatgc ttctacatat atttgaccaa atgcttctac gtgttggtca atgggcacat
      541 gtacaaaaac atcaaagagt ttaaaaagtg cagcattatc gttctcaaac tttatcgttg
      601 tatagctagt ttcaggcaac tcgcttaata gtgcgagaat ctctttttta taatcgccat
      661 tcgcattagc taaaactaag tgagcgtttg gatatttttg taaaatcgtt ttaaaagcag
      721 gtattgtgta ttgtatgcct ttccaataag taaaacgaga aactacacca ataattggag
      781 ctaatttttt tggattgtat ttatgctgta ctgcagcaat ccgcgcttcg tcaacatggt
      841 aaaaatcatt taagtcgaag ccatgtctga tgagtgtaat tttagcctca ggaacatttt
      901 cccattgcac caaaatattt tttacattgt tgcaaatagc aataatatgt gtagaaagtt
      961 tattgattat ccgatcgtac ttaatgccat ttggatggaa ttgatgatgg taacttgaat
     1021 aatgtcttgt gtaaattcgc ttaggaactc ttgctagaaa ggcagctgta atgccgatga
     1081 gtccaccttc aaaaatgtga gcatgcacgg catcaggttt tgtttttctt aaaacagaat
     1141 aaacctgaaa aacacaacgt ggaatatcct tttttgagcg atatgaaatt acatgatatg
     1201 taattttttg ttgaacacaa tgagattcta tatgtgtatg ctgatttcca gagataagta
     1261 taaactccag ctgaaaacgt tgtgcatcta tattgtcgat gatccattca aatgccggag
     1321 ctttgtcgat gccaaataag atgtaggtaa ttttgcgctg caagcagatt tttagctcaa
     1381 atataaagca tttaattcag acatcatttt ttcaaatcca aaaatttttg cgacgtcatt
     1441 tttcgctgtc aacactatat tttgtttata tatatcatct tttaaaaacc tgttgcaagc
     1501 acgtgcaatg tcatcagcat ttttataatc tacaacgcta gcattattcg agtttacata
     1561 ttcgcacgca attccggaaa gcgtgcaaac aacaggtaca gctgctgcca ttgcttcaat
     1621 atatacttga ccaaaagctt cgtattcttt gtcggtcgga acgtgtacaa aaatatcaaa
     1681 gcatttatat aaagttttga tgtccttttc gtattcaatt tcgatgtagt tgtcagcagg
     1741 aacttgctgt aataacttgc taatgcgtgc cttttctggt ccataggcat tcgcaataac
     1801 aagtatagct tgtttgtgtt ctgtgataat ttgtttaaat gcttctattg catattgcac
     1861 acctttgcct tcttcatatc gcgacaccat gcctataata ggaaaaccat ttaatttata
     1921 tttttccttc atcaattgta tcttgtttgt gtttgctact tcaaactcag ctagttgtat
     1981 gccgtgataa atcaatgaga ttttatgatc atggaatttt tctttcgtgc aaacgatatt
     2041 ttctacgttt ttgctaataa caataatatt cgtagataaa taattgataa cattgtcgag
     2101 atatacacct tttttgaaat gatcatgatg aaaattagca tgatgccgag tgtggattcg
     2161 cttctttaca ccagcaagtt ttgctgctgc caagccaact aaagatgcat ttactaaatg
     2221 tgtatgtacg atagtagttt tttctttttt aaatattttt ctgacctgaa agatgccttt
     2281 aaaaaaatct tttttggaaa taatttgcaa ttgataacaa acaaaaccct tttgttgtag
     2341 aatagatttt aaagtgtcag tggtatgatc taaaaagata aaagatagat taaaacgatt
     2401 cttgtcaatg gtatcagcaa tccaaatata ttgatgatag ccaccaatgc tacatagtat
     2461 atatgtaaga tttatttttt tcatttatgg tttttgctac tttggtacca agctttaaaa
     2521 taaacaatag acgataaaat accaatagtt ttaaaggtta taatatcaaa tgcagcacga
     2581 tttggcaaga tgtcaaataa ttttttaaac agaaaattaa atggaataaa gcaggtgtaa
     2641 ataatttgtt ttagtcggct gtgtttggtt tcattttttt tgttttcaaa ttgcgacaca
     2701 taacctcgtc tatctctatc taaaaattcg ttgatgtcta aacggtcttt ttgattatgg
     2761 aaacaagtaa tcggtgtgta taaaaaaatc ggtatgtttg cttcatttag ttttttagaa
     2821 agttcaatgt cttcaccttg aaatatgatg ttttcgttgt acttgcctat cgacataaac
     2881 aaggatttc
//
LOCUS       DAOQQV010000190         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_19049_length_2798_cov_5.823186, whole genome shotgun sequence.
ACCESSION   DAOQQV010000190 DAOQQV010000000
VERSION     DAOQQV010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19049_length_2798_cov_5.823186"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..714)
                     /locus_tag="PLL59_12295"
     CDS             complement(94..714)
                     /locus_tag="PLL59_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24636.1"
                     /translation="MQVEVKNTGKTPEQMVAELQNFKRRLPAGVGNIAVRFFKDNITE
                     RQGFLNSSLELWQKGRRMRFRQSHDLLIGKCNLVRGINYSVSGDTVIVNVIGTAAKYA
                     EIQNAGGTITVTDKMRRFFWAKYFEIKGHAEKKYFKENGDKRNLRGTKQLRKRNNDLD
                     EFNYWYSLALAKKVTIPARKFIGNSIALDNEIMKYIEGNLDNIFNK"
     gene            complement(806..1993)
                     /locus_tag="PLL59_12300"
     CDS             complement(806..1993)
                     /locus_tag="PLL59_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HMY24637.1"
                     /translation="MRTPTNKVSPDMIRATALKLFDGVKKGFDGNVTPDNYNIDQYTT
                     LRKMRENVFVFSGFKDYHQIKEMSMLLTDSKGNIRSFDDFYKDVKQVNETYNIHYLQA
                     EYDHAVSTGTMAAKWDKFKTEADLFPMLRYRALHDGRTRQEHLALDGATYPIGHEFWK
                     TYLPPNGWRCRCDVDQVEKQAIREPKSKPVLKPMFRNNVGVDGVVFPNTHPVFDNDEI
                     ISEAAKNGATIARFSDPDENGYQLVYKSMNRKSVMISYEHKLDDIKYNFNKARIITDA
                     NNGVNLKLRPFVETSGIKNPELEFSNGWIGDFKEPEPDVSLKTSIETLLKRAKLQGCN
                     LPIFVINNKYYDKMETIRALNNSLKIGQKEAIKEVWFLFDKKLIKITRDEIYSKKYYN
                     KLP"
     gene            complement(2094..2459)
                     /locus_tag="PLL59_12305"
     CDS             complement(2094..2459)
                     /locus_tag="PLL59_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24638.1"
                     /translation="MKHLHLKTRQNTIEDSLEFKRLFESASNKSLSKEEQGQILRSIR
                     NGELNSIPKLVESLEETILLIIIQLDIDYINVEEQLNEAKKAVFLLAENEINSISSEK
                     FYDFCAWTVKQAILELTIK"
BASE COUNT          821 a          514 c          396 g         1067 t
ORIGIN      
        1 atttgttttt ttggtcgttg aataaatcaa cgtgtttaag ctctgctaat ttactgcgta
       61 aatgttcgca aatagatatg tatgtatgtt ccattattta ttaaatatgt tgtctaaatt
      121 gccttcaatg tatttcatta tctcattatc taacgctatc gaattgccga taaactttcg
      181 tgcaggtata gtcacttttt ttgccaatgc caagctatac caataattaa actcatctaa
      241 atcgttgttt cgtttgcgaa gttgttttgt gcctcgcagg tttcttttat caccattttc
      301 tttaaagtat ttcttttcgg catgaccttt tatttcaaaa tacttagccc aaaagaaacg
      361 gcgcattttg tctgtaacag ttatagtgcc acctgcattt tgtatttcag catatttagc
      421 agccgtgcca ataacgttta caataacagt atcgccactt actgaataat tgatgccacg
      481 aactaaattg catttgccaa tcagcaaatc gtgagattgt ctaaaacgca tacgccgtcc
      541 tttttgccac aattctaagc tactatttaa aaaaccttgc cgttccgtaa tgttgtcttt
      601 aaagaaccgc acagctatat taccaacacc tgcaggcaat cgccgtttaa aattttgcag
      661 ttctgccacc atttgctctg gtgtttttcc tgtatttttt acttctactt gcataattgc
      721 taagttttgt ttaattttgc tgtgggtgtt ggtttatcct ctacccaccg caacaaaaga
      781 caatagcaat attgtctttt gccatttaag gcagtttatt ataatatttc ttgctataaa
      841 tttcgtctct tgtaatcttt attaattttt tatcaaacaa aaaccatact tctttaattg
      901 cttctttttg tccaattttt aaagagttat ttaaagccct tattgtttcc attttatcgt
      961 aatatttatt atttatcaca aaaattggca agttacaacc ttgtaatttt gccctcttta
     1021 aaagagtttc gatagatgtt tttaaactca cgtctggttc aggctcttta aagtcaccaa
     1081 tccaaccatt ggaaaactct aattctgggt ttttaatacc agatgtttca acaaatggtc
     1141 ttagttttaa attaacacca ttattagcat ctgtgattat tcttgcttta ttaaaattat
     1201 attttatatc atctaactta tgttcataac taatcatgac actttttcta ttcatacttt
     1261 tatatactaa ctgatagccg ttttcgtctg gatctgaaaa acgcgcaatt gttgctccgt
     1321 ttttagctgc ttctgaaata atctcatcat tatcaaacac aggatgtgtg ttcggaaaaa
     1381 caacaccatc aacacccaca ttatttctaa acattggttt taatactggt ttgctttttg
     1441 gctcacgaat agcctgtttt tctacttggt caacatcaca acggcatcgc cagccatttg
     1501 gtggcaaata ggttttccaa aattcatgtc ctatcggata agttgcacca tctaaagcta
     1561 aatgctcctg cctggttctg ccatcgtgca aagctctgta tcgcaacatc ggaaacaaat
     1621 cggcttctgt tttgaattta tcccattttg ccgccattgt tcctgtgctt acggcatggt
     1681 cgtattcagc ttgtaaatag tgtatattat atgtttcatt gacctgcttt acatctttat
     1741 agaaatcgtc aaaggaacga atattgcctt tgctatctgt cagcagcata ctcatttctt
     1801 ttatttgatg atagtcttta aatccgctaa acacaaacac attttcacgc attttacgca
     1861 aagtggtata ttggtcaata ttataattgt ctggtgttac gttgccgtca aatccttttt
     1921 ttacgccatc aaataatttt aatgcagtcg cacgtatcat atcaggactt actttattgg
     1981 ttggtgtcct caccaaaaca cgctatttat tcttgtgttt agcatttgag attcccattt
     2041 gcatgggaat gactttccga atggttgact aattataaaa aattcaaatt atttcatttg
     2101 atggttagtt ccaaaattgc ttgtttaact gtccatgcac aaaaatcata aaacttttca
     2161 ctgctaattg agtttatttc attttctgct aatagaaata ctgctttttt cgcttcattc
     2221 agttgttctt ccacatttat ataatctatg tctagttgga taataattaa caatatcgtt
     2281 tcctctaaac tttctactaa tttcggtata gagtttaatt cgccatttct tatagatctc
     2341 aaaatttgtc cttgctcttc tttacttaac gatttattac ttgctgattc aaataagcgt
     2401 ttaaattcca acgaatcttc gatagtattt tgtcttgttt ttaaatgtaa gtgcttcatc
     2461 tgcctaaatt aatcatgtaa gaaacgaaat ataaaaaaaa agactcttgc ttttcattgt
     2521 cttatttcaa tttatatcac aaagcaggaa ttatgatacg gcaaaacatg tcgtactaaa
     2581 aaatattttt actaatagcg caagcaatat tggaaactaa aaaaatgaaa aattaaattc
     2641 agttaaagac aaataaatat cagcgtttat aaaaaatata atctgtgctt aaggcttcga
     2701 tacctgattg tgtaaatgaa gaagcaattt gtgctctatg atacgttgaa tgatttatga
     2761 tatgaaataa aatatcttgg ataatattat tatatgct
//
LOCUS       DAOQQV010000191         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_19637_length_2746_cov_7.238573, whole genome shotgun sequence.
ACCESSION   DAOQQV010000191 DAOQQV010000000
VERSION     DAOQQV010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19637_length_2746_cov_7.238573"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1571
                     /locus_tag="PLL59_12310"
     CDS             216..1571
                     /locus_tag="PLL59_12310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMY24639.1"
                     /translation="MQRYIFPLFFLLLSHIVAEADSTTATNRYLIDKVDAIVNDKIIL
                     SSDIENQLDLLGLRGENETTNRCHLMKQLIYNKILTTQALRDSLPLADEEVEEELNRK
                     INYFISIAGSQEAFEEYYHKTVEQIKDDYREDIREQMLASRMKSKIVGDIKVTPSEVK
                     TYFEKLSKDSLPYFNATIELQQIVMKPRVSDAQQQASKDKAQGILDRLRAGESFELLA
                     SLYSEDIGSAQAGGLLDWASRGTYVKEFEGAAFKLKPNEISDLVKTPFGYHIIQMIER
                     RGDMIQVRHILVRPQTTSRDAEYAQKKLDSVRNDILEDKISFFNAVKLYSQDEQSKNV
                     GGSLLNNETGSTVLDVDKMDPNLYLLADTLSIGGITPAIMYQADDGTPAFRIVKLISK
                     TEPHVADLRIDYDKIQNAAKADKEEQILSRWFTKNMKKTYLMFTDEYEKCTDLESLVS
                     K"
     gene            1578..2543
                     /locus_tag="PLL59_12315"
     CDS             1578..2543
                     /locus_tag="PLL59_12315"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMY24640.1"
                     /translation="MSQFANDVDGIQHLSASYQQLKKEISKVIVGQDDVVKYVLLSIF
                     CDSHSLLVGVPGLAKTLLVKTVSDVLELHFKRIQFTPDLMPSDIVGAEVLNENRQFHF
                     NRGPIFANIILADEINRTPPKTQSALLEAMQERQVTAAGQAHALNLPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNIVLDYPSYDEELQVVKTTTTDTPTTVSKIIGANEIIAFQH
                     LIRRMPIADNVVEYAVKLVASTRPNTAKAATIVNEFVAWGAGPRASQALVLAAKGHAA
                     INGKYSPDIEDVQAVALPILRHRIIRNFKAEAEGVSMEKIIKDLL"
BASE COUNT          961 a          474 c          519 g          792 t
ORIGIN      
        1 catatcaaga aagtattgaa caaaaatggt tggcagaatt aagactaaaa tatccagttg
       61 ttatcaataa agcggtgctt aatgcaatgg taaaaaagcc ataagctcac caaattttct
      121 taaaaatact taacaacttc atcttcttcg tattttgaag caaaatgcta aactaatagc
      181 ttctattttt gtacatttgt gccttaaatt taagcatgca acgttatatt tttcctttat
      241 tttttctgtt gttgagccac atcgtagcag aagcagacag tacaacagca acaaacagat
      301 acttgattga taaagttgat gctattgtca atgacaaaat tattttaagc tctgatattg
      361 aaaaccaatt agacctttta ggtttgcgtg gcgaaaatga gacgacgaat agatgtcact
      421 tgatgaagca attaatatat aataaaatat taacgacaca agctttgcgc gatagtcttc
      481 ctttggctga cgaagaagtg gaagaggagt tgaatagaaa aataaactat tttatcagca
      541 ttgcaggttc gcaagaagca ttcgaagagt attatcacaa aaccgtagaa caaattaaag
      601 acgattatcg cgaagatatt cgcgagcaaa tgttagcctc acgaatgaaa agtaaaattg
      661 taggcgatat caaagtaaca ccatcagaag tgaaaaccta ttttgagaaa ttatcaaaag
      721 acagtcttcc ttattttaat gcgactatag aattacaaca aatagtaatg aaaccacgtg
      781 tgtcagatgc acaacagcaa gcatcaaaag ataaagcaca aggtatttta gatagattga
      841 gagcaggtga aagttttgag cttttggcga gtttatattc tgaagatata ggctcagcac
      901 aagcaggtgg tttgctcgat tgggcatcgc gtggtactta tgttaaagaa tttgaaggcg
      961 cagctttcaa attaaaacca aatgaaataa gcgacttagt aaaaacgcca tttggctatc
     1021 atattattca aatgatagag cgtcgcggcg acatgataca agtgcgtcat attttagttc
     1081 gtccacaaac aacttctcgt gatgcagagt atgcacaaaa aaaattagac tctgtaagaa
     1141 atgatatact cgaagataaa atctcatttt ttaatgctgt aaaattatat tcgcaagatg
     1201 agcaaagcaa aaatgtaggt ggttcacttt taaataacga aacaggaagc accgttttag
     1261 atgtcgataa aatggaccca aatttatatc tattggcaga tacactttct attggtggta
     1321 tcacaccagc aataatgtat caagcagatg atggcacacc agctttcaga atagtaaagc
     1381 tgatttcaaa aactgagcca catgtggcag atttgcgtat cgattacgac aaaattcaaa
     1441 atgcagccaa agcagataaa gaagagcaaa tcttgagtag atggtttact aaaaacatga
     1501 agaaaaccta cttgatgttt actgacgaat atgaaaagtg cacagattta gaatcactag
     1561 tttcaaaata gagtaacatg agccaattcg caaatgatgt agatggaatt caacatttat
     1621 cagcttctta tcagcagcta aaaaaggaaa ttagcaaagt aattgttggg caagatgatg
     1681 tggtcaaata tgtattactt tctatttttt gcgatagcca tagcttgtta gttggtgttc
     1741 ctggtttggc aaaaacactt ttggttaaaa ccgtctcaga tgtgttggag ttgcatttta
     1801 aacgcattca attcacacca gatttaatgc cttcagatat tgttggcgca gaagtgctaa
     1861 acgaaaatag acagtttcat tttaatagag gcccaatttt tgccaacatc attttggcag
     1921 atgagatcaa tagaacacca ccaaaaacac aatctgcatt attggaagcg atgcaagaac
     1981 gacaagtgac agcagccgga caagcgcatg cattaaactt gccatttttt gttttggcaa
     2041 cacaaaatcc aattgaacag gaaggaactt atccattgcc agaagcacaa ttagaccgtt
     2101 ttatgtttaa tatcgtgttg gattatcctt cgtatgatga agaattgcaa gtcgtaaaaa
     2161 cgacaacaac agatacgcca actacagtgt ctaaaattat tggcgccaat gaaatcatcg
     2221 cattccaaca tcttattcgc cgcatgccaa ttgcggataa tgtagtggaa tatgctgtga
     2281 aattagttgc atcaacacga cctaatacag ccaaagctgc cacgattgta aacgagtttg
     2341 tagcttgggg cgccggccca cgtgcctcac aagcattggt gttagctgcc aaaggacatg
     2401 ctgccatcaa tggaaaatat tcgccagata tcgaagatgt acaagcggta gctttgccaa
     2461 ttttgcgtca tcgtatcatt agaaatttta aagccgaagc agaaggcgta agcatggaaa
     2521 aaataatcaa ggatttattg tagatactct ttatactttg aatcaatcga ctttttttat
     2581 tccatttttg agcacaatag catttttcca atttattttt ttcaattcaa accaatctgt
     2641 agggtcgcct tcaaagtcaa aagtttcttt aaagtccagc agccatggtt tgtgtcctca
     2701 cgaaccatta ctattgtggt cgctttcaaa gtcaaaagtt tcttaa
//
LOCUS       DAOQQV010000192         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_19726_length_2739_cov_7.448584, whole genome shotgun sequence.
ACCESSION   DAOQQV010000192 DAOQQV010000000
VERSION     DAOQQV010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19726_length_2739_cov_7.448584"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PLL59_12320"
     CDS             <1..1377
                     /locus_tag="PLL59_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMY24641.1"
                     /translation="PEELDELDRKIRQLEIEREAIKRENDEVKVQDLSKQIAELSEKR
                     NAFKAKWESEKTVVDRIQNEKINIENLKLEAEQAERNGDYGKVAEIRYGKIKEAESNL
                     QKLELELQENKEGNRLMKEEVTSENIAEIVSRWTGIPLNKMLQTEKDKLLNLEEHLHQ
                     RVVGQEEAIIAVSDAIRRSRAGLQDPKRPLGSFLFIGTTGVGKTELAKALAEFLFDDE
                     KSIVRIDMSEYMEKHAVSRLIGSPPGYVGYDEGGQLTEAVRNHPYSVVLLDEIEKAHP
                     DVFNVLLQVLEDGRLTDNKGRVVDFKNTIIIMTSNIGAHLIQQNFENVITDNDIFTAT
                     ETSKVQILESIKRVLRPEFYNRIDEVIMFKPLLQNDIKAIVKIQLHSLQKQLQTMDIT
                     LDFTDELVAHFTAEGFEPQYGARPLKRLINKELINQLSKMIIADKISKDKPVIADAFN
                     GEIVFRNE"
     gene            complement(1438..1821)
                     /locus_tag="PLL59_12325"
     CDS             complement(1438..1821)
                     /locus_tag="PLL59_12325"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HMY24642.1"
                     /translation="MPTFSILFAAIAALIHVLFFLAESIFWMQPKVHRVFGVKNIQEA
                     ELFKISFFNQGFYNLFLAIGVFVGMYFIHSENIIIGKTLLLFCCASMLSAAIVLFLSK
                     PKLIIGALVQGLCPLISILAFLFLK"
     gene            complement(1897..>2739)
                     /locus_tag="PLL59_12330"
     CDS             complement(1897..>2739)
                     /locus_tag="PLL59_12330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HMY24643.1"
                     /translation="YLLHGDTMSLLPEFEHKFDMVFADPPYFLSNNGLSIQNGEIVSV
                     NKGKWDKSEGFEFINDFNRKWLSLVRDKMKDDATIWISGTMHNIFSVGQILTELGFKI
                     LNIVTWEKTNPPPNFSCRYFTYSTEQIIWARKSERVPHYFNYELMKQLNGDKQMKDVW
                     KLAAIAPWEKSCGKHPTQKPLSVLTRLILASTKPNAWILDPFAGSSTTGIAANLANRR
                     FLGIEQEEEFLRISKNRKLEIQNPKIAATYRQKTGGFNNKKEYELFLVEEPRTEYKTE
                     INLG"
BASE COUNT          945 a          445 c          501 g          848 t
ORIGIN      
        1 cccgaagaat tagatgagtt agatagaaaa attcgtcaac ttgaaattga acgagaagcc
       61 attaaacgcg aaaatgatga agtaaaagtt caagacttgt caaaacaaat tgcagagtta
      121 agtgaaaaac gaaatgcatt taaagcgaaa tgggaaagtg aaaaaacggt agtcgataga
      181 attcaaaatg aaaaaataaa cattgagaat ctaaaattag aagcggaaca ggccgaacga
      241 aatggtgatt acggaaaagt agctgaaatt cgttatggta aaataaaaga agccgaatcc
      301 aacttacaga aactggaact ggaattacaa gaaaataaag aaggcaatcg cttgatgaaa
      361 gaagaagtga caagcgaaaa tattgcggaa attgtgtcgc gatggacagg cattccactt
      421 aataaaatgt tgcaaacaga aaaagataaa ctccttaatc tggaagaaca tttgcatcaa
      481 cgtgtcgtcg gccaagagga agctatcatc gccgtttcag atgccattcg cagaagtcgt
      541 gctggcctgc aagatccaaa acgaccatta ggttctttct tgtttatcgg tactacaggt
      601 gttggcaaaa cagaactggc aaaagcatta gcagagtttt tatttgacga tgaaaagtct
      661 atcgtgcgta tcgatatgag tgagtatatg gaaaaacatg ccgtgtcgcg cttgattggt
      721 tcgcctcctg gatatgttgg ttacgacgaa ggcggtcaac tcactgaagc ggttcgcaat
      781 catccataca gcgtggtctt gttagatgaa attgaaaaag cacatcctga tgtttttaat
      841 gtattattac aagtcttaga agatggcaga ttgactgata ataaaggtag agtagttgat
      901 tttaaaaata cgattatcat catgacttcc aatattggcg cgcatttgat tcagcaaaat
      961 tttgaaaatg taataacgga taatgatatt tttacagcaa cagaaacctc aaaagtgcaa
     1021 atcttagagt caataaagcg tgtgttacgt ccagagttct ataaccgaat tgatgaagtg
     1081 attatgttta aaccactttt gcaaaatgat attaaagcaa ttgtgaaaat tcaacttcat
     1141 agcttgcaaa agcaattaca aacaatggat atcacactcg attttacaga tgaattggta
     1201 gcacatttta cagcagaagg ttttgaaccg caatacggtg cgcgtccgtt aaaacgtttg
     1261 ataaataaag aattgatcaa tcaactgtct aaaatgatta ttgctgataa aatttctaaa
     1321 gataaaccag tcattgcaga tgcatttaat ggcgaaatcg tttttagaaa tgaataatct
     1381 ccattgcatt caataattac atcacatcat aagcagtaca ggttgtactg ctttttttta
     1441 tttcaaaaat aaaaatgcga gaatcgaaat cagcggacat aagccttgca ctaaggcacc
     1501 gataattaat tttggttttg aaagaaatag cacgatggca gcagacaaca tcgatgcaca
     1561 gcaaaaaaga agtagtgttt tgccaataat gatattttca ctatgtataa aatacatacc
     1621 aacaaataca ccaatagcca aaaataaatt ataaaaacct tgattgaaaa atgatatttt
     1681 gaataattcc gcttcttgta tattttttac accaaagacg cgatgtactt ttggctgcat
     1741 ccaaaatatg ctttccgcca aaaaaaataa cacatgaatc aatgcagcaa ttgcagcaaa
     1801 tagtatagag aatgtcggca tgagagtaaa attttttatg atttaatatt ataattttgg
     1861 tctaaaagat tgaaaatatt tttttctttc atcgatttaa cctaagttta tttctgtttt
     1921 atactctgtc cttggttctt caactaaaaa taattcatat tcttttttgt tgttgaaacc
     1981 tccagttttt tgtctgtatg ttgctgcaat ttttggattt tggatttcca attttctgtt
     2041 tttgctaatt ctcaaaaatt cttcttcttg ttcaattcct aaaaatcgtc tgttagccaa
     2101 attagctgca attcctgttg tcgaactacc tgcaaatgga tctaaaatcc aagcattagg
     2161 ttttgtagat gccaaaatta gtcttgttaa aacagacaat ggtttttgag tcggatgttt
     2221 gccacacgac ttttcccaag gtgcaatcgc tgctagcttc caaacatctt tcatttgttt
     2281 gtcgccattg agttgtttca tcaattcgta attgaaataa tgtggaactc tttcgctttt
     2341 tcttgcccaa ataatttgtt cagttgagta tgtaaagtat cgacatgaaa aatttggtgg
     2401 cggattggtc ttttcccaag ttacgatgtt aagaatttta aaacctaatt cggttaagat
     2461 ttgtccaact gaaaaaatat tgtgcatggt gccacttatc caaatagttg catcatcttt
     2521 cattttatct ctaactaatg aaagccactt gcgattgaag tcgttgataa attcaaagcc
     2581 ttcggatttg tcccatttcc ctttgtttac gcttacgatt tctccgtttt gaatggaaag
     2641 tccattgttt gataaaaaat aaggcggatc ggcaaaaacc atatcaaatt tatgttcaaa
     2701 ttcaggcaag aggctcattg tatctccgtg aagaagata
//
LOCUS       DAOQQV010000193         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_20623_length_2668_cov_5.261003, whole genome shotgun sequence.
ACCESSION   DAOQQV010000193 DAOQQV010000000
VERSION     DAOQQV010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20623_length_2668_cov_5.261003"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1479)
                     /gene="der"
                     /locus_tag="PLL59_12335"
     CDS             complement(166..1479)
                     /gene="der"
                     /locus_tag="PLL59_12335"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HMY24644.1"
                     /translation="MSYTVAIVGRPNVGKSTFFNRLLEQRKAITDDFSGVTRDRQYGI
                     SEWNGKVFNVIDTGGFVLHSDDVFEKAIRQQVRLAIQEADLMILMVDVTTGITDLDES
                     LTQELRKTKKPVILAVNKVDNTERSYAANEFYALGFENLFSVSSMSGSGTGEILDFIT
                     EQIPDDIENAIDIYADLPKIAIVGQPNAGKSTLLNALVGEERNIVTEIAGTTRDSIHT
                     HYNLYGKEFLLIDTAGIRKKSKVHEDLEFYSVIRAVKAIEESDVCILMLDATVGIELQ
                     DLKIFSIIEQRKKGVVLLVNKWDAIEKDSKTMLEFEKRIKEKIAPFTDVPIVFISALE
                     KQRIYQAIDKVMEVYTNKQIKVPTHKLNDVMLAEIEKYGPPAYRGEYIKIKYVQQLPS
                     TTPSFAFFCNHPDYIKEPYRNYLENRMRENFKLTGVPINIFFRKK"
     gene            complement(1605..2489)
                     /gene="era"
                     /locus_tag="PLL59_12340"
     CDS             complement(1605..2489)
                     /gene="era"
                     /locus_tag="PLL59_12340"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HMY24645.1"
                     /translation="MNHKAGYVNILGKPNVGKSTLMNALVGERVSIITSKAQTTRHRI
                     LGIVNGNDFQIVFSDLPGIIKPAYKMQESMMDFVRTSLEDADVFIYMAQIGDKPEKQP
                     DEYEKIKQMKIPVIILLNKMDIAEHEEVEQERILWQSDFPNAKVIPFSAKFNWNLETL
                     MNELVANLPESEPYFDKEDWTDRPERFFVTEVIREKILLNYKQEIPYSCEVVCSYFME
                     DGDIIKISVEIYVERESQKPILIGKGGEMLKKVGTQARLDLEKFFGKKIFLETHVRVS
                     ENWRNNESMLRKFGYRDY"
     gene            complement(2587..2659)
                     /locus_tag="PLL59_12345"
     tRNA            complement(2587..2659)
                     /locus_tag="PLL59_12345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2624..2626),aa:Met,seq:cat)
BASE COUNT          828 a          504 c          416 g          920 t
ORIGIN      
        1 gaaacgcttc atattcatta tgcaaattaa aaggcgtatt taatttagtg tttacatgca
       61 ttcaaaatca ttatacgaaa aaactggagt tcctaacttt gagtggacaa tataaataag
      121 agaataaagg cgaactttat ggtatgatga caactggtta aattactatt tttttctaaa
      181 gaaaatatta ataggaacgc cagttaattt aaaattttcg cgcattctat tttctaagta
      241 gttgcgatat ggttctttaa tataatctgg atgattgcag aagaatgcaa atgaaggcgt
      301 agtgctcggc aattgttgca catactttat ctttatatac tcgccacgat aggctggcgg
      361 accatacttt tcaatttctg ccaacatcac atcattgagt ttatgtgtag gcactttaat
      421 ttgcttgttt gtatatactt ccatcacttt atcaatcgct tgataaatac gttgtttttc
      481 caaagctgaa ataaatacaa tcggaacatc tgtaaatggt gcaattttct ctttgatgcg
      541 tttttcaaat tcaagcatcg ttttagagtc tttctctatt gcgtcccatt tattaaccaa
      601 taaaacaaca ccttttttac gttgctcaat aatgctaaat attttcaagt cttgtaattc
      661 tatgccaact gtagcatcaa gcatcaaaat acaaacatca gattcttcga ttgcttttac
      721 agcacgaatg acagaataaa attccaaatc ttcgtgtact ttactttttt tgcgaatgcc
      781 agctgtatct atcagtaaga attcttttcc atataaatta taatgtgtat ggatagaatc
      841 tcgtgttgtg ccagcaattt ctgtaacaat gtttcgctcc tcacctacta atgcattcag
      901 caatgtagat tttccagcat taggttgccc tactatggca atttttggta aatctgcata
      961 gatatcaatt gcattttcaa tatcatcagg aatctgctct gtaataaaat ctaatatttc
     1021 gccggtgcca ctgccactca tcgacgaaac ggaaaataaa ttttcaaaac ctaaagcata
     1081 aaattcattt gcagcatacg aacgctctgt attatcaact ttatttacag caagtataac
     1141 tggctttttc gtcttgcgta attcttgtgt taatgactca tctaaatcgg tgatgcctgt
     1201 tgtaacatca accattaaaa tcattaaatc ggcttcttgt atggctaaac gcacttgctg
     1261 gcgtatggct ttttcaaaaa cgtcatcaga gtgtaataca aaaccacctg tatcgataac
     1321 attaaacacc ttaccattcc actcgctgat gccatattgc ctatctctgg ttacaccact
     1381 aaagtcatct gtaatggctt tgcgttgttc taataatcga ttgaaaaaag tagattttcc
     1441 tacattggga cgacctacga tagctacagt atatgacacg gcgataattt atttgtttgt
     1501 aaaatagttg ataaaagaat caacttgcaa agttatgtat aaatgataga aatgctttat
     1561 ttataagtag aataaaatat tattactttg ttacacctta ctaattaata atcgcgataa
     1621 ccaaatttac gaagcatgct ttcgttattg cgccagtttt ctgacacgcg aacatgtgtt
     1681 tccaaaaata tttttttgcc gaaaaatttc tctaaatcca atcgagcttg cgtgcctacc
     1741 tttttaagca tttcaccacc tttgcctatc aatattggtt tttggctttc ccgctctaca
     1801 tatatttcta ctgaaatttt tatgatgtcg ccatcttcca taaaatagga acacacaact
     1861 tcacagctat atggaatttc ttgtttatag ttgagtaata ttttttcacg tataacttct
     1921 gtaacaaaaa aacgttcagg tctatctgtc cagtcttctt tatcaaaata aggctcactt
     1981 tctggcaaat tagcaacgag ttcattcatt agcgtttcta aattccaatt aaattttgca
     2041 gaaaacggaa ttactttcgc attcggaaaa tctgattgcc acagtatgcg ctcttgttct
     2101 acttcttcgt gttcggcaat gtccatttta ttcaataaaa tgataaccgg aattttcatt
     2161 tgttttatct tctcatactc gtctggttgc ttttctggtt tatcgcctat ttgtgccatg
     2221 tagataaata catcagcatc ttctaaggaa gtacgcacaa aatccatcat cgattcttgc
     2281 atcttgtaag caggtttaat gataccaggc aaatcagaaa atacaatttg aaagtcattg
     2341 ccgttgacaa tgcctaaaat acgatgtctg gtggtttgtg cttttgaagt aataatagaa
     2401 acgcgctcgc caacgagtgc attcattaat gtagatttgc caacgtttgg cttacctaaa
     2461 atatttacat atccagcttt atggttcata aggtgatcta aattttataa aaaccctaaa
     2521 atttatatac taatttacaa aaaaaataaa aaaatggctc ataaaaagag ccattttttc
     2581 aagtagtagc gggaacagga ctcgaacctg tggccttcgg gttatgagcc cgacgagcta
     2641 ccaactgctc catcccgcga tgtggtgc
//
LOCUS       DAOQQV010000194         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_20671_length_2664_cov_8.605596, whole genome shotgun sequence.
ACCESSION   DAOQQV010000194 DAOQQV010000000
VERSION     DAOQQV010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20671_length_2664_cov_8.605596"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1738)
                     /locus_tag="PLL59_12350"
     CDS             complement(218..1738)
                     /locus_tag="PLL59_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24646.1"
                     /translation="MNKFLAIIILIFVQVSFAQNNTAFNYIDVNQAALTVSTAQTGGN
                     YFNAYNLSVLDMSHQLADIFKKGEVNKEYYLNKLIKVTKVFYTKDNSRMLIIQPVYDN
                     ASKRCILLTNGNGENFNWSMSNRTAIDYALRGYVVAYYENVGSVAGRFDGKGNTVNYY
                     TAKVINQVGAITAKDKFFASMYVNLFLTNAARKFMADNSEKVGIDTTKFYLSGGSLGA
                     NASLFFTYADGNNFTHPLFNAVKNKLGFNQPINNNGIVAVAAFGGGLPGPAEGLGTII
                     DQQDKTPAFFFSGSLDWVVNPNKTTILGPENWGPLALRSMFDANHIPYNTYINAYGTH
                     VFQTPSFNQQWSSLPAIQTAGTANEKVASGNVDAYAKKNMINLLMYQYENTQMHEAGK
                     MAASYFDQAANNSTPKSAVTFVEPKCIQNTIFYQYSIGKLSIENAAKYFTTSPNNPEI
                     NKSLDKITSTCLDGTFQLYSKGATAPIVPDNFMAPGKGLRKLANFLEILNQLNKKK"
     gene            complement(1842..2294)
                     /locus_tag="PLL59_12355"
     CDS             complement(1842..2294)
                     /locus_tag="PLL59_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HMY24647.1"
                     /translation="MSLDQNINNELKEAMLSKNEIKLRTIKAIKAAFLIAKTEKGASG
                     EITAEQEIKILQKLFNQRKESFDIFTKENRPELAIKEKEEMDVISSYLPKQLDDEALK
                     SIVQASISETGASSIKDMGKVIGMVSKKVAGQAEGARISAMVKELLGA"
     gene            complement(2382..2597)
                     /locus_tag="PLL59_12360"
     CDS             complement(2382..2597)
                     /locus_tag="PLL59_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24648.1"
                     /translation="MFRFFKYIVLFILGYKALKMLFAQMQTPEKVEPAPPRQNINNNQ
                     QQQQQHYNTAAKQKFNDAELIDYEEVK"
BASE COUNT          794 a          483 c          458 g          929 t
ORIGIN      
        1 agtggtttgt tggttacaaa ccaacaaagg cgtagaaaat aatatagtga aaccactttc
       61 actatattat accgctaaca cctaccagca ctacatctaa gggcattttt acgcaccatt
      121 tttgaccaca ttgtctttaa aaatgtcttt ccttactgtt tagtgagaaa agacattttt
      181 cttttagtga acactcagaa atgatagcac actgtttcta ctttttctta ttgagttgat
      241 ttaatatttc taagaaattt gccaatttgc gtaagccttt tcctggtgcc ataaaattgt
      301 ctggcacaat tggcgcagtc gcacctttac tgtataattg aaaagtacca tctaagcaag
      361 tagatgtaat tttatcaagg cttttgttga tttcaggatt gtttggactt gtcgtaaaat
      421 atttagccgc attttctata cttaattttc cgatactata ttgatagaaa atcgtgtttt
      481 gtatgcattt tggctcaacg aatgtaacag cagattttgg tgtagaattg ttagctgctt
      541 ggtcaaaata gcttgctgcc atcttgccgg cttcatgcat ttgtgtgttc tcatattgat
      601 acatcaataa gttgatcata ttcttttttg cataagcgtc aacgttgccg cttgctactt
      661 tttcgtttgc tgtgccagca gtttgtatgg ccggcaaact actccattgc tggttaaaag
      721 atggcgtttg aaaaacatgc gttccatagg cattgatgta cgtattatac ggaatatgat
      781 tagcatcaaa catacttcta agtgctaacg gaccccaatt ttcgggtcct aaaatggtgg
      841 ttttattcgg attgacgacc caatctaaag agccactaaa aaagaaagca ggtgttttat
      901 cttgctggtc aatgatggtg cctaagcctt ccgctggacc aggaagtccg ccaccaaaag
      961 ccgctactgc tacaatgcca ttgttgttga ttggctgatt gaaacctaat ttatttttta
     1021 ctgcattaaa taatggatgt gtaaaattat tgccatcagc atacgtaaaa aataaagaag
     1081 cattagcacc caaactgccg cctgacaaat agaactttgt tgtgtcaatg ccgactttct
     1141 cactattgtc tgccataaat ttgcgtgccg cattggtcaa aaataaattg acatacatag
     1201 aggcaaagaa cttgtctttt gctgtaatag caccaacttg attgattact tttgctgtat
     1261 aataatttac ggtattgcct ttgccatcaa atcggcctgc aacactgcct acattttcat
     1321 aataagccac tacatagcca cgtaatgcat agtcaatggc agtacgattg gacatcgacc
     1381 aattaaaatt ttcgccattg ccattcgtca acagaataca gcgttttgat gcattatcat
     1441 atactggctg aatgattagc attctgctat tgtctttggt gtagaaaact ttagtgacct
     1501 taatgagctt gtttagataa tattctttat tgacttctcc ttttttaaaa atatcagcca
     1561 attgatgtga catgtctaaa acagacaaat tataggcatt aaaataattg ccaccagttt
     1621 gtgctgtaga tactgttaaa gccgcctgat tgacatcgat atagttaaat gcagtattat
     1681 tttgagcgaa agatacttgt acaaatatca aaatgatgat ggccaaaaat ttattcatat
     1741 tccatttttt taagtataga tacaattatt ataccataaa aataaacaaa gcctatctaa
     1801 taaatggcat ttaacgacta aataagaaat taaagcgaaa tttatgcgcc aagcagttct
     1861 tttaccattg cagaaatgcg agctccttcg gcctgcccgg ctactttttt gctgaccatt
     1921 ccgattactt ttcccatgtc tttaatggaa gaagcgcctg tttctgaaat acttgcttgt
     1981 acaatacttt ttagtgcttc atcgtctaat tgcttaggca aatacgagga gattacgtcc
     2041 atttcttctt tttctttaat ggctaattca ggtcgatttt ctttagtgaa aatatcgaat
     2101 gactctttgc gctggttgaa tagcttttgt aagattttta tttcttgttc agccgtaatt
     2161 tcaccacttg cacctttttc tgttttggca attaaaaatg cggctttgat ggccttgata
     2221 gtacgcaatt ttatttcatt cttagaaagc atcgcttcct ttaattcgtt gtttatattt
     2281 tgatcgagac tcatattaat ttaaaaattt agaaatgaaa caatttaata aggcaaaatt
     2341 actgaattca gcatggattt actaaaaacc aatactaata actacttcac ttcttcgtag
     2401 tctatcaatt cggcatcatt aaatttctgc ttggcagcag tattgtaatg ttgctgctgc
     2461 tgctgttgat tgttgttgat attttgacgt ggtggcgccg gttctacttt ttctggtgtt
     2521 tgcatttggg caaacagcat ttttaatgct ttataaccta gaataaataa gacgatgtat
     2581 ttaaaaaatc gaaacatgat acaaaattag tagtttacta tcataataga acattaaata
     2641 ggcgaaaata gtttcgcaca ccaa
//
LOCUS       DAOQQV010000195         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_21230_length_2621_cov_5.511302, whole genome shotgun sequence.
ACCESSION   DAOQQV010000195 DAOQQV010000000
VERSION     DAOQQV010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21230_length_2621_cov_5.511302"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..621)
                     /locus_tag="PLL59_12365"
     CDS             complement(55..621)
                     /locus_tag="PLL59_12365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY24649.1"
                     /translation="MLSVILPTYNRFEIVEETLQKIVTLETNIPFEIIVVNDGQELPF
                     NMQHEKLSIYKNPKKGASAARNFGASKAKYDIFFFIDDDMWISVESIEAIAQLYQQHF
                     FDKQCVNLNWQYPKSLCDQMRNEKIGRYLLHANYHTLEGRSKIKVDSTSSLVPVTSMG
                     SGSFAIGKSVFESIGKYNENILFQGEDI"
     gene            complement(614..1597)
                     /locus_tag="PLL59_12370"
     CDS             complement(614..1597)
                     /locus_tag="PLL59_12370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMY24650.1"
                     /translation="MSLPKISIITPSYNQGQFIEQTIDSVLSQGYPNVEYIIIDGGST
                     DNSVEIIKKYAKHISYWISEKDNGQSDAINKGLKMASGDVINWLNSDDYYEPNALLTI
                     GQYFEDDSIHVLAGKSKIWKNDSYFISPGTDIYPTVEKTIGYARIDQPETFFRKSVID
                     KIGFLNTNLHYVMDKEWWIRYLMQYGLKNVKKTDDVLVNFRIHESSKTNSQKVQFIQE
                     TFDLYYTLAYMNNLEEASLMDKNFRVNYIDGLSDIHQQHTSTMQCALHYFLLLHFRTM
                     YAQNNYQEAKKTGLLLNEKYLQEADLHELYKLRKRIKYLPLFLKKLWNKNA"
     gene            1673..2599
                     /locus_tag="PLL59_12375"
     CDS             1673..2599
                     /locus_tag="PLL59_12375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMY24651.1"
                     /translation="MSATPANIPTCLFCGDTKTTFTCFEDAVFNNKTFQYLKCCNCDL
                     IYVYPSPTDEDLMKMYPVEYQGAFTEKGTGRYTTLFKQIRDLGTFKTVLDYGCGGGRF
                     VVEAIDEKYEVVGAEYNPALVANLKSAFPSASFQTINDLFASQQKFDIIFLSNVVEHL
                     TQPKETMLQLMQHLNDNGIFVLEGPVENNFNLTLLTRKTLFFIRKYLFHKKAYHTPTH
                     LLYSNRKNQEQLFKDVGLTTLFYTVDESNWPFPSALSECKSTMQKLMYYIATFSVKMS
                     KHFSFWGNNFTYIGKKRLAKFYYTLSIIRIAA"
BASE COUNT          801 a          451 c          430 g          939 t
ORIGIN      
        1 aatcggtgtg tataaaaaaa tcggtatgtt tgtttcattt agttttttag aaatttaaat
       61 gtcttcacct tgaaacaaga tattttcgtt gtatttccca atagattcaa acacagattt
      121 tccaattgcg aatgagccgc ttcccattga tgtaactggt actaagctcg atgtactgtc
      181 cacttttatt tttgagcgac cctctaatgt gtgataattt gcatgtaaga gatagcgtcc
      241 gattttttca ttcctcattt gatcacaaag tgattttgga tattgccaat ttagatttac
      301 acattgtttg tcgaagaaat gttgttgata tagttgtgct atggcctcaa tgctctcgac
      361 actaatccac atatcatcat caataaaaaa gaagatatcg tatttagctt tcgatgcacc
      421 aaagttacgt gcagcgcttg cgcctttttt gggattttta taaatactta atttttcatg
      481 ttgcatgtta aatggcaatt cttgaccatc attcaccacg ataatttcaa acggaatgtt
      541 tgtttctagg gtaacaattt tttgtagagt ttcttctaca atttcaaaac gattatatgt
      601 cggcagtata acactaagca tttttattcc ataatttttt taaaaataaa ggcaaatatt
      661 ttatgcgttt tctcagcttg tacagttcat gtaagtctgc ctcctgtaag tatttttcat
      721 tgagtaataa tcctgttttt ttggcttctt gataattgtt ctgcgcatac atagttctaa
      781 aatgcaacag taaaaaataa tgtaaagcac attgcattgt cgatgtatgt tgttggtgaa
      841 tgtctgaaag accatctatg taatttaccc taaaattctt atccattaag cttgcttctt
      901 ctaaattgtt catataggca agtgtgtagt ataagtcaaa tgtttcttgt atgaactgca
      961 ctttttggct atttgttttt gagctttcat gtattctgaa attgaccaac acatcatcag
     1021 ttttctttac attttttaag ccatattgca tcagatagcg tatccaccat tctttgtcca
     1081 ttacataatg taaattggtg tttaaaaatc ctattttatc tatcacactt tttctaaaaa
     1141 atgtttcagg ttggtcgata cgcgcatagc caatggtttt ttctacagtt ggatatatgt
     1201 cggttccagg cgaaataaaa taggaatcat ttttccatat tttacttttt ccagcaagca
     1261 catgtatgct gtcatcttca aaatattgtc caattgttag taatgcattc ggttcgtagt
     1321 agtcatcact attcagccaa ttgatgacat cgccacttgc catttttaag cctttgttga
     1381 tggcatcgct ttgcccatta tctttttcgc ttatccaata cgaaatatgt ttcgcatatt
     1441 ttttgataat ctctacagaa ttatctgtac ttccgccatc aataataata tattctacgt
     1501 ttggatagcc ttgacttaat acagaatcaa tagtctgttc aataaattga ccttgattat
     1561 aagaaggagt aataatgcta atttttggca aggacatgtt ggcaaaaata agcatatcgt
     1621 tgcaaataaa gcaatgaaca aaatatcaaa gtaattttta tctttgtttc cgatgagcgc
     1681 aacaccagcc aatataccta catgtttgtt ttgtggcgat acaaaaacaa cttttacttg
     1741 ctttgaagat gctgttttta acaacaaaac atttcaatat ctaaagtgtt gcaattgcga
     1801 tttaatatac gtgtatcctt cgccaacaga tgaggattta atgaaaatgt accctgtaga
     1861 atatcaaggc gcatttaccg aaaaaggcac aggcagatat accaccttgt ttaaacaaat
     1921 acgtgatttg ggtacattta aaacagtatt agattatggt tgtggcggag gaagatttgt
     1981 agtagaagcc attgacgaaa aatacgaggt tgttggtgcg gaatataatc cagccttagt
     2041 ggccaatcta aaatcagcat ttccaagtgc aagctttcaa acaataaacg acttattcgc
     2101 ttcccaacaa aagtttgata tcatcttctt gagcaatgtt gtggaacatc tcactcaacc
     2161 taaagaaacc atgttgcagc tcatgcaaca tttaaatgac aatggcattt ttgtattaga
     2221 aggtcctgtt gaaaacaact ttaatcttac cttgctaaca cgaaaaacct tgttttttat
     2281 tcgtaaatat ttgttccaca aaaaagcgta ccatacacca acgcatttgt tgtactccaa
     2341 tcgtaagaac caagagcaat tgtttaaaga tgtaggttta acaactttat tttatacggt
     2401 tgatgaaagc aattggccat ttccatcagc gctgtcagaa tgcaaatcta cgatgcagaa
     2461 attgatgtat tatattgcta ctttttctgt gaaaatgagc aagcactttt cgttttgggg
     2521 aaataatttc acttatatag gcaaaaaacg actcgctaaa ttttactata cgttgagcat
     2581 tattcgtatt gctgcttgaa atgcttgtgt gctattgtta g
//
LOCUS       DAOQQV010000196         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_21496_length_2600_cov_6.915128, whole genome shotgun sequence.
ACCESSION   DAOQQV010000196 DAOQQV010000000
VERSION     DAOQQV010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21496_length_2600_cov_6.915128"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1342)
                     /locus_tag="PLL59_12380"
     CDS             complement(23..1342)
                     /locus_tag="PLL59_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24652.1"
                     /translation="MHLSTAKKLFLLFACLFFCKAPLFADKKKKSASQYEKPNIIKRA
                     WGDITTRNNYYFNANELYKEVVRNYEFNRVINYNTLLPFYYHDQADFSSNTADMETVA
                     KKTGIVLQLHDYSRWRDNAFLLLGKSQFLRKQYDTSLVTFQYIVTTMKPGKMNLKLEY
                     SNRDRLKYIRKRQKELEKKMGEKKKFIEFKFKEQQKEAEEKAEAARDKQEAAIAKKKK
                     ELEEIIKAKKKIIALQKKGKKIPQELIEKAKSKTTKVDSTVLHANTSVKKSDKPKFDS
                     NQPYVLLGDQYVANPYYKDTTGERKNQNPLKELDPKKEAKFDKLTFWEKIKHKLSRPE
                     AIVWMAKSLIELENYADAKSMISYGKALRKLTKKQRRDFYLIDAYYNIRRKDYSPAID
                     ELETAMIYFKKKKDKAYYEYLLAQLYQLNNQDADAADYFVKAGKHTK"
     gene            1461..2285
                     /locus_tag="PLL59_12385"
     CDS             1461..2285
                     /locus_tag="PLL59_12385"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMY24653.1"
                     /translation="MRLKNVYRFQIVDEKSYDVKLVMELNPLNIIIGTGLILAFFTFL
                     NFVLIAFTPLKQYIPGYGSSSGRREIINMGIKAEELDNSIKAHQKYVANLQNILNDKV
                     VIDAVKSDLKKSKVDTGILSIKTTDESQFVKEIEKGLKNAALLESVRDANISALSRLE
                     LIQPVSGNAIIKHSDKNIGTTFSASENEPVKAVLAGNVVATSYSAEDGYTIIVQTENQ
                     LVYILKNNSQLLKKNGNFVQEGETIANAGKFKNKAFVSTLELWYRGHAIDPQKFIK"
     gene            2290..>2600
                     /locus_tag="PLL59_12390"
     CDS             2290..>2600
                     /locus_tag="PLL59_12390"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMY24654.1"
                     /translation="MFSKIAPKSNLETMVANKINDGTIINGNIVAQTDLRIDGKVAGD
                     IQCAAKVVIGATAQVAGQIKCNDLTVEGKVKGNLDINGTLYFRSTALYEGDVKYKKLI
                     V"
BASE COUNT          811 a          448 c          455 g          886 t
ORIGIN      
        1 atcaagatgc agatgcagca gattatttgg tatgcttacc agcttttaca aaataatctg
       61 ctgcatctgc atcttgattg ttcagttgat aaagttgtgc taataaatat tcgtaataag
      121 ccttatcttt tttcttttta aaataaatca ttgccgtttc caactcatca attgctggtg
      181 aataatcttt tcggcgaata ttgtaataag cgtcaatcaa ataaaaatct ctacgttgtt
      241 tttttgtcaa tttgcgcaaa gccttaccgt atgaaatcat gctttttgca tccgcataat
      301 tttctaattc tattaatgat ttcgccatcc aaacaatagc ttctggacgt gaaagtttgt
      361 gtttaatttt ttcccaaaat gtcaatttat caaacttcgc ttcttttttc gggtcgagtt
      421 ctttaagtgg attttgattc tttcgttcgc ctgtagtgtc tttgtaatat ggatttgcaa
      481 catattgatc tccgagcaac acataaggct gattgctatc aaacttcggc ttatccgatt
      541 ttttgactga agtatttgca tgcaaaacag tagaatctac tttagttgtt ttgctttttg
      601 ccttttcaat taattcctgc ggaatttttt tacctttttt ctgtaaggca ataatcttct
      661 ttttggcttt aataatttct tctaattctt ttttcttttt ggcaatggct gcttcttgtt
      721 tgtcgcgcgc tgcttccgct ttttcctcgg cttctttttg ctgttcttta aatttgaact
      781 cgatgaattt tttcttctcg cccatttttt tctctaattc tttctgccgc tttctgatgt
      841 attttaaacg gtcgcgattg ctatattcta atttcaaatt catcttacct ggtttcatgg
      901 tggtcacgat gtattgaaat gttactaaag aagtatcgta ttgcttgcgt aagaattgtg
      961 atttacctaa aagtaaaaaa gcattatcac gccatctgct ataatcatgt aattggagta
     1021 caatacctgt tttcttcgcc accgtttcca tatcggcagt atttgacgaa aaatcagcct
     1081 gatcatgata atagaatggc aaaagcgtat tatagttaat gacacgatta aactcataat
     1141 tgcgcactac ttctttatag agttcattgg cattaaaata ataattgtta cgtgtagtaa
     1201 tatcacccca agcacgctta atgatattag gcttttcgta ttgcgatgct gattttttct
     1261 ttttatcggc aaacaatggc gccttacaaa agaaaagaca agcgaaaagc aagaatagtt
     1321 tttttgcagt agatagatgc atgctcgttt taattcccta attttaacgc aatttcttaa
     1381 atttatttta cttattcgag tgaaatttat ttaattctac acagatggct ataaaaaaac
     1441 caaattggaa actatggaag atgcgtctca agaacgtcta tcgcttccaa attgtggacg
     1501 aaaagagcta cgatgtgaaa ttggtaatgg aattaaatcc gctaaatatc atcattggta
     1561 caggtttaat attagcattt tttacgtttc ttaattttgt attgattgca tttacgccat
     1621 taaaacaata cattccaggc tatggctcat catcaggaag gcgtgaaatt atcaatatgg
     1681 gaattaaagc agaagaatta gacaattcta tcaaagcaca tcaaaaatat gttgccaatt
     1741 tgcaaaacat cttaaatgac aaggtcgtca ttgatgctgt aaaatctgat ttaaaaaaat
     1801 caaaagtcga taccggcatt ttgagtatta aaacaaccga tgagtctcaa tttgtaaaag
     1861 aaatagaaaa aggactgaaa aacgccgcct tattagagtc ggttcgagat gcgaatatta
     1921 gtgcactgag ccgtttagaa ctcatacagc ctgttagcgg aaacgcaatt atcaaacatt
     1981 ctgataaaaa tataggaacc actttctctg ctagcgaaaa tgagcctgta aaagccgtat
     2041 tggcaggcaa tgtggtggcg accagctatt ccgcagaaga tggctataca attatcgtac
     2101 agactgaaaa tcaattggtt tacatattaa aaaacaatag tcaattatta aagaaaaacg
     2161 gtaactttgt acaagaagga gaaaccatcg ccaatgctgg aaaattcaag aataaggcgt
     2221 ttgtttctac gctcgaactt tggtatagag ggcacgcaat tgaccctcag aaatttataa
     2281 aataaaagta tgttctctaa aatcgcacct aaaagcaact tggaaaccat ggttgccaat
     2341 aaaataaatg acggcactat tataaatgga aatattgttg cgcaaactga cttgcgcatc
     2401 gatggtaagg ttgctggcga tatacaatgt gctgctaaag tggtcattgg tgctacagct
     2461 caagtagctg gtcaaattaa gtgcaatgac ttgactgttg aaggaaaggt gaaaggcaat
     2521 cttgatatca atggtacact ttattttaga agcactgccc tatatgaagg cgatgtaaaa
     2581 tataaaaaac tcattgtcga
//
LOCUS       DAOQQV010000197         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_21765_length_2580_cov_8.310099, whole genome shotgun sequence.
ACCESSION   DAOQQV010000197 DAOQQV010000000
VERSION     DAOQQV010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21765_length_2580_cov_8.310099"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLL59_12395"
     CDS             complement(<1..541)
                     /locus_tag="PLL59_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HMY24655.1"
                     /translation="MNKQIKNIIEKILGIKKLETNEEQMDKATMSFYISIKSSIKEIA
                     FMLVGIFSAAFGLKGFLLPNDFLDGGATGISLLISELTDIPIYILIIVVNIPFIILGI
                     KAVNKTFAVKTAISILGLSLVLATVSFPEITNDKLLISIFGGFFLGAGIGMAIRGGAV
                     IDGTEVLAVYLGRRSATSIG"
     gene            complement(577..1488)
                     /gene="xerD"
                     /locus_tag="PLL59_12400"
     CDS             complement(577..1488)
                     /gene="xerD"
                     /locus_tag="PLL59_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HMY24656.1"
                     /translation="MGKWKLYIKNFEAYLKIEKSLSQNSVDAYLHDVELLEQYTELND
                     NNASPGDLELKNFENFIAHLHDLGLSDRSQARIISGLKAFYKYLLTDEYVSKDPTTLL
                     EGPKLGKKLPDTLSFEEIEKLIAALDLSKPENVRNKAMLETLYSSGLRVSELIQLKIS
                     HIYFDVEFIRVIGKGNKERLIPIGCEALKHIKIYIEHIRNKMPQHQQYSDILFLNKHG
                     KPISRVMVFLVIKSLAEKIALQKNISPHTFRHSFATHLIEGGADLRAVQQMLGHSSIT
                     TTEIYTHLDQQYLKESLTLHHPRFMHH"
     gene            1576..1992
                     /gene="aroQ"
                     /locus_tag="PLL59_12405"
     CDS             1576..1992
                     /gene="aroQ"
                     /locus_tag="PLL59_12405"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HMY24657.1"
                     /translation="MKRIIIINGPNLNLLGKREPTVYGSDTFEDFLADMQQKYANLEI
                     SYFQSNIEGELISKLHEIGFSYDGIILNAAGYTHTSIAIADAIRAIKTPVVEVHISNT
                     FSRETFRHHSFVSPVSKGVIVGFGLKSYELALKYFE"
     gene            complement(1998..>2580)
                     /locus_tag="PLL59_12410"
     CDS             complement(1998..>2580)
                     /locus_tag="PLL59_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24658.1"
                     /translation="YTATNYEFGKQTTMNAIKTCANSAQGQHVHAIVDNGPYTAVYKN
                     NVQLKAKNDGQHIVLSFLSRSYHESIKNKNAYALTSVITGMSRYTARYAAPELDKPML
                     FYSRPKGEYTGIETDAVLLDFYLVNCDLSKKGYKVKAEINGTTFMLDKWCGYFMEGLP
                     MGENTVKLTLLDSKGNTVESPYSVSERKFILKK"
BASE COUNT          780 a          451 c          413 g          936 t
ORIGIN      
        1 cgccaattga agtggctgaa cgcctgccta aatatacagc taatacttct gtgccgtcaa
       61 tcacagcacc acctctaatg gccattccaa tgccagcacc taagaaaaat ccaccaaaaa
      121 ttgaaatcaa taatttatcg tttgttattt ctggaaaact caccgttgcc aataccaatg
      181 acaaccctaa tatagaaatt gctgttttta cagcaaatgt tttatttaca gctttaatgc
      241 ctaatatgat aaaaggaata ttgacaacaa taattaaaat ataaattgga atatctgtta
      301 attcggaaat taacagcgaa atacctgtgg cgccaccatc taaaaaatca tttggcaata
      361 aaaatccttt taaaccaaag gcagcagaga aaataccaac cagcataaat gctatttctt
      421 ttatgctgct ttttattgaa atatagaaag acattgttgc cttatccatt tgctcttcat
      481 tagtctcaag ctttttaata cctaaaattt tttcaatgat attttttatt tgtttattca
      541 tacgtcgttt tcaatataca aaaatttttc taatttttag tgatgcatga aacgtggatg
      601 atgtagtgtt aatgattctt ttaaatattg ctgatctaaa tgcgtatata tttctgtcgt
      661 agtaatcgat gagtgtccca gcatttgctg cactgcacgt aagtcagcgc ctccttcaat
      721 taaatgtgtt gcaaatgaat gtcgaaatgt atgtggcgaa atattttttt gcaatgctat
      781 tttttctgct aaacttttta tcactaaaaa taccattacg cgcgatatag gtttgccatg
      841 tttgtttaaa aataaaatat ctgaatattg ttggtgttga ggcattttgt tgcgaatatg
      901 ctcaatgtaa atttttatat gttttagtgc ttcgcaacca attggaatta gcctttcttt
      961 attgcctttg ccaatgacac gaataaactc tacatcgaaa tagatatgag aaattttgag
     1021 ttgaataagc tcggacacac gcaaaccact gctgtataag gtttcgagca ttgctttatt
     1081 gcgcacgttt tctggtttgc ttaggtcgag tgcagctatt aatttttcta tttcttcaaa
     1141 tgataaagtg tcaggcaatt tttttccaag ctttggtcct tctaataatg ttgttggatc
     1201 tttgcttaca tattcatcag tcagtaaata tttataaaat gccttcaaac cagatatgat
     1261 acgtgcttga cttctatcgc tcaatcctaa atcgtgaaga tgtgcaataa aattttcgaa
     1321 attttttaat tctaaatcgc ctggtgaggc attgttatca ttgagttcag tatattgctc
     1381 taataattca acatcgtgca ggtaggcatc aacagagttt tgtgataaag atttttcaat
     1441 ttttaaataa gcttcaaaat ttttaatata caatttccat ttgcccatat caagcactaa
     1501 gatagtattg tgataagtat ttttctccta ttttttttat atcttgtagc attaaacata
     1561 cataaattta atttcgtgaa acgcattatc attatcaacg gacccaactt aaacttactc
     1621 ggcaaaagag aaccaactgt ttatggttct gatacgttcg aagatttttt agctgatatg
     1681 cagcaaaagt atgcgaatct tgaaatttcg tatttccaaa gtaatattga aggtgagctg
     1741 atttcgaaat tacacgaaat aggattttct tacgatggaa ttatcttgaa tgcagctgga
     1801 tatacacata catcaattgc cattgccgat gccatacgag ctatcaaaac gccagtcgtt
     1861 gaagtacata ttagtaacac attttcgcga gaaacattta gacatcattc tttcgtttca
     1921 ccagtatcta aaggtgttat cgttggcttc ggtttaaaaa gctatgagct ggctttaaaa
     1981 tattttgaat aattttctta tttcttcaaa ataaatttgc gttcagacac actgtaagga
     2041 ctttctactg tattgccttt actgtctaaa agtgttaatt ttactgtgtt ttcgcccatt
     2101 ggcaagcctt ccatgaaata gccacaccat ttatctaaca taaatgtagt tccatttatt
     2161 tccgctttta ccttatatcc ctttttagac aaatcacaat tcactaaata gaaatctaat
     2221 aaaacggcat ctgtttctat accagtatat tcgccttttg gtctgctgta aaataacatt
     2281 ggtttatcta attctggcgc tgcgtatcgt gcagtatatc tcgacatgcc agtaattact
     2341 gatgtcagcg cgtacgcatt tttgtttttg atgctttcgt gataggagcg cgataagaat
     2401 gataatacga tatgttgtcc atcatttttt gcttttagct gtacattatt tttatatact
     2461 gctgtgtatg gtccattgtc cacaatggcg tgtacatgtt gcccttgtgc agagttggca
     2521 cacgttttta tggcattcat tgttgtttgc ttaccaaatt catagtttgt agctgtgtaa
//
LOCUS       DAOQQV010000198         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_22771_length_2515_cov_6.589024, whole genome shotgun sequence.
ACCESSION   DAOQQV010000198 DAOQQV010000000
VERSION     DAOQQV010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22771_length_2515_cov_6.589024"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLL59_12415"
     CDS             complement(<1..205)
                     /locus_tag="PLL59_12415"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220699.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HMY24659.1"
                     /translation="MSKYSSLLGEERAKYLLEHQCKTIDKSLLHLPCPDFTEKIWIQS
                     NRSPQVLRSLQQLYGTGRLANTGY"
     gene            complement(262..1020)
                     /locus_tag="PLL59_12420"
     CDS             complement(262..1020)
                     /locus_tag="PLL59_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HMY24660.1"
                     /translation="MRIVSLVPSITLLLIDLGLEDSIVGRTKFCTLPIDNVRNIPTIG
                     GTKNVDIDKIKALKADFIIANKEENVKEQIEALKIYCKVILFDVRNLDENYKMIAKIS
                     KLTNTNEKAHDIIKQTQANFEKLQTHIAPLKALQLNAVYMIWKKPYMTVGKDTFIHAM
                     LEHLGVKNMFSHQTRYPIIQQFNTSYFEKCNLVFLSSEPYPFEEKHIREFQTMLPNTK
                     IILVDGTYFSWYGSKMIDAPKYYQQLAEKISAVS"
     gene            1197..1790
                     /locus_tag="PLL59_12425"
     CDS             1197..1790
                     /locus_tag="PLL59_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24661.1"
                     /translation="MKNQITIALAAILFLAVSCKEEEIHPPITKVVNIRDTVKIAFDT
                     VFLNDSSVVLYSDWLTPTSTSGTGTGSGPGTGTGTPSTPSMVRSCTFNAPYITQKIID
                     KGVILAYCKLDGDAMTRALATTTMVNGFISIWDYAVSVGKIQFIQTTGNPAGIPAISS
                     KHQFRYVVIPPSRNIRLSKPYSLMSYDEICQLFNIPK"
     gene            1985..2512
                     /locus_tag="PLL59_12430"
     CDS             1985..2512
                     /locus_tag="PLL59_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24662.1"
                     /translation="MKKLIFATGLIALLSVAACQKDQEEPETEDQFKDNTPEYPISTS
                     RLKYYSYDQEFMLSFNHIGTTINLSDIQDNVLKNSSVLTFFKDDNCVGIWSKVGGNNC
                     SNEFKTSTGIENRTLYFNVQHTDGSDYDNHEKTLLKIKVYVIPKKTKTILKVNDNLQD
                     LSYEDVRALLQIKEE"
BASE COUNT          878 a          404 c          393 g          840 t
ORIGIN      
        1 cgtaacctgt atttgccaat cggccagtgc catagagttg ttgtaaacta cgtaaaacct
       61 gcggtgacct attactttgt atccaaattt tttctgtaaa atctggacac ggtagatgta
      121 ataatgattt gtcaatggtt ttacattgat gttctaacaa gtacttagct ctttcttcgc
      181 ctaatagact gctgtattta ctcataaatt tatttttaac ggtgcaaaga taaacaatat
      241 agtgcgtatt ggatatgctt tttaagagac tgcgcttatc ttttctgcta attgttgata
      301 atattttggt gcatctatca tcttactgcc ataccacgag aaataggtgc catcgaccaa
      361 tataattttg gtatttggta gcatagtttg aaattctcga atatgttttt cctcaaatgg
      421 atatggctca gaggaaagga atactaaatt gcatttttca aaataggacg tattaaattg
      481 ctgaataata gggtagcgcg tttgatgtga aaacatattt ttcacgccta agtgttccaa
      541 catagcatga ataaacgtgt ctttgccaac cgtcatatat ggctttttcc aaatcatata
      601 tactgcattt aattgtaatg ctttgagtgg cgcaatatga gtttgtaatt tttcgaaatt
      661 agcttgtgtt tgtttgataa tatcgtgtgc tttttcgtta gtattagtaa gctttgaaat
      721 tttagcaatc atcttatagt tttcatctaa atttcgaaca tcaaacaaaa ttaccttaca
      781 atatatttta agtgcttcaa tttgctcttt tacgttttct tctttattgg caataataaa
      841 atcagctttt aatgctttta ttttatcaat atcaacgttt tttgtgccgc caattgttgg
      901 aatatttctt acattatcaa ttggtaatgt gcaaaatttt gttctgccta caatactatc
      961 ttctaaccct aaatcaatta gcaatagtgt gatagaagga actaatgata caatacgcat
     1021 agctaatttt tataatatat atgtcagtac tttgatgtaa aagtataaaa aatggtcaaa
     1081 aaataacaga aattaccttt tactcaaagt atttaccatt tactaattac acaaaacaga
     1141 tattgttaaa ataatcaaaa tttacgttat caaaatcacc ataaaaaatt acaattatga
     1201 aaaatcaaat caccatcgca ttagcagcta tcttgttctt agctgtatct tgcaaagagg
     1261 aagaaatcca tccaccaatt acaaaagtgg taaacatcag agacactgta aaaattgctt
     1321 ttgacacagt atttctaaac gacagttccg tagtgttata ttcggactgg ctaacaccta
     1381 caagcacatc aggcacgggc acaggtagtg gacctggcac aggcactggt acgccaagta
     1441 caccttcaat ggttcgtagc tgtactttta atgcgcctta cattacacaa aaaatcatag
     1501 acaaaggtgt aatattggct tattgtaaac tagatggcga tgcaatgact agagcattag
     1561 caaccacaac aatggtaaat gggtttatca gtatttggga ttatgcagtt agtgtcggta
     1621 aaattcaatt tattcaaact acgggtaatc ctgctggcat tcctgccatc agtagcaaac
     1681 accaattccg ttatgtcgtt attcctccat ctagaaatat acgattaagc aaaccgtatt
     1741 ctctaatgag ctatgatgaa atatgtcaat tgtttaatat tccaaaataa agtatagatc
     1801 tcaattgtta aaactactca gcgtgctgag tagtttttta tttaaaaaca tatgaatttt
     1861 accattaaag aaatttaaaa gagagaaatg aacaactcaa aaccacttac tcattcgatt
     1921 tgacaatagt tcttttctgt atcaacttta gatatttctt tttcaaacca taaacaaaat
     1981 ttctatgaaa aaactaattt ttgcaacagg cttgatagca ttattaagtg ttgcagcatg
     2041 ccaaaaagat caagaagaac cagaaacaga agaccaattt aaagacaata ctcctgagta
     2101 tcctataagc acatctcgtt taaaatacta tagttacgat caagaattta tgctttcatt
     2161 taatcacatt ggtacaacta taaatttaag tgacatacaa gacaatgtac ttaaaaatag
     2221 ttcggtgcta actttcttta aagatgataa ttgtgttggc atctggagta aggtaggcgg
     2281 aaataattgt tccaatgaat ttaaaaccag tacaggtatt gaaaacagaa cactctattt
     2341 taatgtacag catacagatg gaagcgacta tgataaccat gagaaaacat tactaaaaat
     2401 aaaagtatat gttattccta aaaagactaa aacaatttta aaagtaaatg ataatttaca
     2461 agatttaagt tatgaagatg tacgtgcatt acttcaaata aaagaggaat agggt
//
LOCUS       DAOQQV010000199         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_23610_length_2459_cov_6.329035, whole genome shotgun sequence.
ACCESSION   DAOQQV010000199 DAOQQV010000000
VERSION     DAOQQV010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23610_length_2459_cov_6.329035"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLL59_12435"
     CDS             <1..1020
                     /locus_tag="PLL59_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H)"
                     /protein_id="HMY24663.1"
                     /translation="SLIQVKNIKSYDEALDTIGKGDGCEVCRPAVSSLLASIWNDLVV
                     AKTHHASQDTNDRFLANIQKGGTYSVVPRIPGGEITPEKLMAIGTVAHKYNLYTKITG
                     AQRVDMFGAHLNDLPYIWEELIAAGFESGHAYGKSLRAVKSCVGSTWCRFGLHDSVSF
                     AIQIENRYKGIRAPHKLKGAVSGCVRECAEAQGKDFGIIATEKGWNLYVCGNGGAKPQ
                     HAQLIASDIDSETCIKYIDRFLMFYIKTADPLTRTAPWLNKLEGGINYLKEVIIHDSL
                     GIAADLEAEIATLIETYQCEWKAAIENPEIRKRFSHFANAKDEKDPSITFAPMREQKK
                     ATNWG"
     gene            1048..1410
                     /gene="nirD"
                     /locus_tag="PLL59_12440"
     CDS             1048..1410
                     /gene="nirD"
                     /locus_tag="PLL59_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HMY24664.1"
                     /translation="MEVQEKKADTAVKVWFAACKTEDVIDNGGACIKYEDQQIAVFYF
                     TRRNEWYATQNECPHKKQMALSRGMLGSHGDTPKVACPFHKKTFSLQTGECLSGDECA
                     IKTYPVKIDNGMVYIGIS"
     gene            1490..2311
                     /locus_tag="PLL59_12445"
     CDS             1490..2311
                     /locus_tag="PLL59_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HMY24665.1"
                     /translation="MDYVTPKEVAGTMLSAALTKSSLATKDLLIRGMLSGALLAISVT
                     IAFTAVNQTSMPLMGAIVFPVGFIVIILLGLELVTGSFAIIPIAYMEKQISAKKMLNN
                     LFWVFIGNLIGSLLYAILYWAAITTLGANDSSDPIAQMIIKASEKKTIGYMSFGSAGA
                     MTMFIKAILCNWMVCMGVVMSMTSKSTIGKVIAAGMPIFMFFSLGFEHAVVNMFLIPA
                     GILFGANVSIADWWYNQSIVTLGNIVGGLLFTGLAVFYTFNIKAKNTEVELQKAS"
BASE COUNT          865 a          429 c          474 g          691 t
ORIGIN      
        1 agcctcatac aagtaaaaaa cataaaatct tacgacgaag cattagatac cattggaaaa
       61 ggtgatggct gcgaagtttg tcgccctgcg gtgagctctt tattggctag tatttggaac
      121 gatttagtag tagcaaaaac acatcacgct tcacaagata ccaacgaccg atttttggca
      181 aatattcaaa aaggtggcac ctactctgta gtacctcgaa tacctggtgg cgaaattact
      241 ccagagaaac tcatggccat aggcactgta gcacacaaat acaacctata tacaaaaatt
      301 acaggtgcgc aacgtgtaga tatgtttggt gcacacctaa acgacttacc ttatatttgg
      361 gaagaactca tcgccgctgg atttgaaagt ggccatgctt atggcaaatc attacgtgcc
      421 gtaaaaagct gtgtaggctc tacatggtgc cgattcggat tacacgatag cgtgagcttt
      481 gccattcaaa tagaaaatag atacaaagga atacgtgcac cacacaaatt aaaaggagcc
      541 gtttcaggtt gtgtgagaga atgcgcagaa gcacaaggaa aagactttgg tattattgct
      601 acagaaaaag gctggaattt atatgtatgc ggtaatggtg gtgctaaacc acaacatgct
      661 caactaatcg catctgatat agatagtgag acctgcataa aatacatcga tagattttta
      721 atgttttata ttaaaacagc cgacccactt acaagaacgg cgccttggct caataaatta
      781 gaaggcggta taaactattt aaaagaagta atcattcacg acagtcttgg cattgcagca
      841 gatttagaag ctgaaatagc tacacttatc gaaacctatc aatgtgaatg gaaagcagct
      901 attgaaaatc cagaaatcag aaaaagattt agccattttg caaatgcaaa agatgagaaa
      961 gatccttcga taacatttgc tcccatgaga gagcaaaaaa aagcgactaa ttggggttga
     1021 tataattaaa aaataaaaaa aaagtatatg gaagtacaag aaaaaaaagc agatacggca
     1081 gtaaaagtat ggtttgcagc ttgcaaaaca gaagacgtaa ttgacaatgg tggcgcatgt
     1141 attaaatatg aagaccaaca aattgcggta ttttatttta cacgaagaaa tgaatggtat
     1201 gccacacaaa atgagtgccc acacaaaaag caaatggcac ttagcagagg tatgctagga
     1261 agccatggtg acacgcctaa agtcgcttgc ccatttcata aaaaaacatt ctccttacaa
     1321 actggtgaat gcttaagtgg agatgaatgt gctataaaaa catatcctgt aaaaattgat
     1381 aatggtatgg tgtatatcgg catttcataa taaaaaaaca acgctaaatc tttagataca
     1441 aaaacttatt tcaatcaata tttaattatt aaaactaaaa cacgcacaca tggattacgt
     1501 aacaccaaaa gaagtagcag gcacaatgct tagtgctgca ctcacaaaat catctttagc
     1561 tacaaaagac ctattaatcc gtggaatgtt gtctggtgca ctcctcgcca tctccgttac
     1621 cattgcattt acggcggtca accaaactag tatgccgctc atgggcgcta ttgtatttcc
     1681 tgtaggcttt atagttatta tcttactagg tttagaactc gtaaccggta gttttgccat
     1741 cattccaatt gcctatatgg aaaaacaaat ttctgccaaa aaaatgctta ataatttatt
     1801 ttgggtattt ataggcaatc ttatcggcag cttgctttat gccatattat attgggctgc
     1861 cataacaaca ttaggtgcga atgatagcag cgaccctatt gcacaaatga ttatcaaagc
     1921 atcagaaaag aaaactattg gctatatgtc atttggcagt gcaggcgcaa tgacgatgtt
     1981 tatcaaagcc attttatgta attggatggt atgtatgggc gtagtaatgt ctatgacctc
     2041 aaaatctacc attggaaaag taattgctgc cggaatgccc atttttatgt ttttctcttt
     2101 gggatttgag catgcagttg tcaatatgtt tttaattcct gcaggcattc tatttggtgc
     2161 aaatgtatcg atagccgact ggtggtacaa tcaatctatt gtaacattag gaaatattgt
     2221 aggtggatta ttatttacag gcttagctgt attttacacc tttaatataa aagctaaaaa
     2281 cactgaagta gaattgcaaa aagcaagtta attatccaat tccaacttat ttaatccaag
     2341 tatatgaaaa tgaaattatt ttcaatagga atcctattgc tattttgtaa tgcactttat
     2401 gcatcaaaga tagatagtat aaaaatgaac ttcgacttta gaacgagatt agaagtaga
//
LOCUS       DAOQQV010000200         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_23818_length_2446_cov_6.820995, whole genome shotgun sequence.
ACCESSION   DAOQQV010000200 DAOQQV010000000
VERSION     DAOQQV010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23818_length_2446_cov_6.820995"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..960)
                     /locus_tag="PLL59_12450"
     CDS             complement(1..960)
                     /locus_tag="PLL59_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24666.1"
                     /translation="MKKIILCLLLLKGVVYAGKPSNFKLPLVVSKDSVEIVRLPKDFD
                     HLSVRDFTISPKNDEIFFTVESNKNTIATIMHMIKKNGKWYLDVAPFSGNFYDLEATF
                     TPDGNSLFFASNRPLDSDSTIKDFDIWKTDKINGVWQTPKNLGNVINTEADEFYPAIT
                     NSGTLYFTAQYKHSKGKEDIWMSKYVNGNYTTPESISDSVNTALYEFNAFVSPNDSII
                     IFTSCGRSDDLGGCDLYISKKEANGIWSKAKNLGSKINSDKLDYCPYISFDNKFFVFT
                     SNRTKLQKSYKQKLNIDTFLKEMQQVQNGKGNLYWINADEVLK"
     gene            1002..2348
                     /gene="accC"
                     /locus_tag="PLL59_12455"
     CDS             1002..2348
                     /gene="accC"
                     /locus_tag="PLL59_12455"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMY24667.1"
                     /translation="MFQKILIANRGEIALRIIRTCKEMNIKTVAVFSTADRDSMHVTC
                     ADESFCIGPPAGKDSYLKMDSILMAAEITKADAIHPGYGFLAENETFARKCAEKNIKF
                     IGPTPEQIHAMGDKITAKETMIKAKVPVIPGSEGLITDIAKGKKLAKEIGYPVILKAT
                     AGGGGKGMRIVWNESEFQSNLENAQAEAGAAFGNAGMYLEKYIEEPRHIEVQVAGDQF
                     GNAAHFSERDCSIQRRHQKLVEESPSPFVDDALRKKLGDAAVKAVKAIHYEGVGTIEF
                     LVDKHKSFYFMEMNTRIQVEHPVTEEVVDVDLIRLQIELAAGKKLEKAEYFPQGCAIE
                     CRINAENPYDNFKPSPGKILNYQASGGYGVRMDSHVYNGYIIPPFYDSMIAKLIVKGN
                     TRLEAIDRMKRALHELKINGIKTTIPFHLQLMDNEDFRKGNFTTKFLEDFEMNEEE"
BASE COUNT          796 a          432 c          473 g          745 t
ORIGIN      
        1 ttattttaat acctcatcag cattaatcca atataaattt cctttgccat tttgtacttg
       61 ttgcatttct tttagaaaag tatctatgtt taatttttgc ttatacgatt tctgaagctt
      121 agttctattg cttgtaaaca caaaaaattt gttgtcaaaa ctgatataag gacaatagtc
      181 taatttgtca gaatttattt tcgatccaag attttttgct tttgaccaaa tgccattggc
      241 ctctttttta ctgatatata aatcgcagcc gcccaaatca tcgctacgtc cgcacgaagt
      301 gaaaataatt atagaatcat ttggtgaaac aaaggcatta aactcataca aagccgtgtt
      361 taccgaatca gaaatacttt ctggtgttgt atagtttcca tttacatatt tgctcatcca
      421 aatgtcttct ttaccttttg aatgcttgta ttgagcggtg aaatatagcg taccactgtt
      481 tgttatcgca ggataaaatt catctgcttc tgtattaatt acatttccta aatttttcgg
      541 tgtttgccaa acgccattta ttttatctgt tttccatata tcaaaatctt ttattgtgct
      601 atcactgtcc aagggtctgt tcgaagcaaa aaacaaacta tttccatcag gtgtaaatgt
      661 cgcttctaag tcataaaaat ttccagaaaa tggtgctaca tctaaatacc atttcccgtt
      721 tttctttatc atgtgcataa tagtagctat cgtattttta ttactttcta ccgtgaaaaa
      781 tatttcatca ttttttggag atatcgtaaa atctcttact gaaagatgat caaaatcttt
      841 cggcagacgc actatttcca cgctatcttt cgaaactact aatggcaatt tgaagttact
      901 aggtttgcca gcatacacaa caccttttaa cagtaacagg cacaagataa tcttcttcat
      961 tttctaaaaa taaaacaaaa tgtagttaaa ttagcatact tatgtttcaa aaaatactca
     1021 tagccaaccg tggtgaaatc gctttgcgca tcattcgcac ttgcaaagaa atgaatataa
     1081 aaacggttgc tgttttctct actgccgata gagacagtat gcacgttacc tgtgctgatg
     1141 aaagcttttg tattggacca cctgccggaa aagattcata tctaaaaatg gatagcattc
     1201 taatggcagc tgaaattaca aaagcagatg ccattcatcc tggttatggc ttcttggcag
     1261 agaatgaaac attcgcgaga aaatgtgcag agaagaatat taaattcatc ggaccaacgc
     1321 cagaacagat acacgcaatg ggcgacaaaa tcacagcaaa agaaaccatg ataaaggcaa
     1381 aagtgcccgt aattcctggc tctgaaggct tgattacaga tattgccaaa ggtaaaaaac
     1441 tagcgaaaga aattggctat cctgttatct tgaaagcaac tgcaggcggc ggcggaaaag
     1501 gcatgcgcat cgtttggaat gaaagcgaat ttcaatccaa cttagaaaat gcacaagcag
     1561 aagcaggagc agcatttggc aatgctggta tgtatttgga aaaatatatc gaagaaccac
     1621 gacacataga agtgcaagtg gctggcgacc agttcggcaa tgcagcccat ttttcagaac
     1681 gagattgttc gatacaacgc cgacatcaaa aattagttga agaaagtcca agtccatttg
     1741 ttgatgatgc tttgcgcaaa aagcttggcg atgctgcggt aaaagccgta aaagcaattc
     1801 actacgaagg cgttggcacc atcgagtttt tagtggataa gcacaaaagt ttttatttta
     1861 tggaaatgaa tacacgtata caagtggagc atccagtaac agaagaagta gttgatgtgg
     1921 atttaattcg tttacagata gaacttgctg cgggaaagaa actagagaaa gctgaatact
     1981 ttccgcaagg ttgcgccatt gaatgccgta tcaatgcaga aaatccgtat gataatttca
     2041 aaccgagtcc tggaaaaatt ttgaactatc aagccagtgg cggttatggt gtacgcatgg
     2101 attcgcatgt gtataatggt tatatcattc cgccgtttta tgattctatg atagcgaaac
     2161 tgattgtaaa aggcaatacg cgattagaag cgattgatag aatgaaacgc gctttacacg
     2221 aattaaaaat caatggtata aaaactacga ttccttttca tttgcaatta atggataacg
     2281 aggatttccg caaaggaaat ttcacgacga agtttttaga ggattttgag atgaatgagg
     2341 aagagtgaat tgtttcgctt ggtgggcgac gacaagcgat agagaaaatg gttcgtgagg
     2401 aaaattgggt gtgtgatttt atgttgtttc ttgaggacac gaacca
//
LOCUS       DAOQQV010000201         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_30363_length_2118_cov_8.397964, whole genome shotgun sequence.
ACCESSION   DAOQQV010000201 DAOQQV010000000
VERSION     DAOQQV010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30363_length_2118_cov_8.397964"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..716
                     /locus_tag="PLL59_12460"
     CDS             87..716
                     /locus_tag="PLL59_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMY24668.1"
                     /translation="METTALFKEIFEPKLIKELTEKAKPISAKAGDIILDFGETVRQI
                     PIILSGSVKVSRIDEEGKELLLYYVNPKESCAMTFTCCMQQYPSEIKAVAEEDVEMLA
                     IPIAVMDQWLVKYPSWKSFVMKTIHHRFNELLKAIDQIAFQKLDDRLISYLKEKSKAT
                     KSTLINLSHQQISEELATSRVVISRLLKKLENEKKILLYRNQIKLLKDL"
     gene            776..985
                     /locus_tag="PLL59_12465"
     CDS             776..985
                     /locus_tag="PLL59_12465"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HMY24669.1"
                     /translation="MKQNMGKIDKAVRMSLAVLIAVLYFANIISGALAIILLVFATVF
                     ILTSFIGTCPLYLPFNINTNENKKA"
     gene            1002..1316
                     /locus_tag="PLL59_12470"
     CDS             1002..1316
                     /locus_tag="PLL59_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMY24670.1"
                     /translation="MISQLKSMFGLGPKIDFKLAMQNGAQIIDVRTKAEYQSGHIKGS
                     LNIPLNNLSNHYAKLDKSKTIITCCASGIRSAQAQNILQANGFKEVYNGGGWYGLQEK
                     LK"
     gene            1318..1605
                     /locus_tag="PLL59_12475"
     CDS             1318..1605
                     /locus_tag="PLL59_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24671.1"
                     /translation="MLQTIKYNLFHNWNAIRIIRLVLSVIIIVQAVQIHDVLFGLLGV
                     FFLYQALSNTGCCAMNTCSPTPTTNSNTQEVAVEFEEIKNNKDGKNAEEII"
     gene            1586..1888
                     /gene="trxA"
                     /locus_tag="PLL59_12480"
     CDS             1586..1888
                     /gene="trxA"
                     /locus_tag="PLL59_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMY24672.1"
                     /translation="MQKKSFKEYIQDNKPVLVDFYADWCGPCRVMHPILEEVKAKLND
                     DVTVLKINIDKNARVASSYQIQSVPTLILFKNGQIKWRQSGVVPANQLEKIIQQNK"
     gene            1896..>2118
                     /locus_tag="PLL59_12485"
     CDS             1896..>2118
                     /locus_tag="PLL59_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24673.1"
                     /translation="MTTAHLYDVSVQWNEDRKGTMSSTVLTQKIEVATPPEFAKGMPN
                     IWSPEHLFVAAVNSCLMTTFLAIAENSKLE"
BASE COUNT          800 a          341 c          361 g          616 t
ORIGIN      
        1 aacagcttta aaattagata gcgaacgcag aatgaccatg cgttaaacaa gtgcttaatt
       61 atttcattat ataatgagta aaaactatgg aaacaacagc attatttaaa gagatatttg
      121 aaccaaaatt aatcaaagaa ttaactgaaa aagccaaacc catttcagca aaagctggtg
      181 atattatctt ggattttggt gaaacagtgc gacaaattcc tattatactc agtggttctg
      241 taaaagtttc gcgcatagac gaagaaggaa aagaattgtt attgtattat gtaaatccga
      301 aggaaagttg cgccatgaca tttacttgtt gtatgcagca atatccgagc gaaataaagg
      361 cagtggcaga agaagatgta gaaatgcttg caataccaat agcagtaatg gatcaatggt
      421 tggtgaaata tccgagctgg aaaagttttg taatgaaaac cattcatcat cgttttaatg
      481 aactgctaaa agcaatcgat caaattgcat ttcaaaaatt agacgatcgc ttgatttcct
      541 atctaaaaga aaaatctaaa gccaccaaat ccactttaat taacttatcg catcaacaaa
      601 tatcagaaga gcttgccact tctcgtgtgg taatttctcg attgctaaag aaactggaaa
      661 acgaaaaaaa gatattatta tacagaaatc aaattaagct acttaaagat ttgtaacatt
      721 tgtaactgac aatcgcaatt ttgctagcta attttgtttt ataaaattca ttgttatgaa
      781 acaaaatatg ggaaaaatag acaaagctgt acgcatgtcg ctagctgttt taattgcagt
      841 tttgtatttc gcaaacatca tcagcggagc actcgcaatc attttattag tatttgctac
      901 tgtgtttata cttacaagtt ttattggcac ttgtccatta tacttgcctt ttaatatcaa
      961 caccaacgaa aataaaaaag cttaatctta aaaaaaacaa tatgatctct caattaaaaa
     1021 gtatgtttgg cttaggtcca aaaatcgatt ttaaacttgc tatgcaaaat ggcgcacaaa
     1081 ttattgacgt acgcacaaaa gcggaatatc aatcaggcca cattaaaggc tctttaaata
     1141 ttccattaaa caatctatct aatcattatg caaaattaga taaaagcaaa accattatta
     1201 cttgttgcgc ttctggtatt cgcagtgcac aagcacaaaa catcttacaa gccaatggtt
     1261 ttaaagaagt ctataatggt ggcggctggt atggtttaca agaaaaatta aaataatatg
     1321 ttgcaaacaa tcaaatacaa tttatttcat aattggaatg ccattcgcat aatacgttta
     1381 gtattgagtg tcatcattat tgttcaagct gtgcaaatac acgacgtgtt atttggttta
     1441 ttaggcgttt tctttttata tcaagcactg agcaataccg gttgttgcgc aatgaatact
     1501 tgctcaccaa cgcctacaac aaattccaat acgcaagaag ttgctgttga atttgaagaa
     1561 ataaaaaata ataaagatgg aaaaaatgca gaagaaatca tttaaagaat acatacaaga
     1621 taataaacca gtcttagttg atttctacgc agattggtgt ggaccttgcc gagtaatgca
     1681 tccaatttta gaagaagtga aggcgaaatt aaatgacgat gttactgttt taaaaataaa
     1741 catcgataaa aatgcgcgtg tcgcatcaag ctatcaaatt caaagtgtgc ctactttaat
     1801 tttatttaaa aatggccaga taaaatggcg acaatcaggt gtggtgccgg caaatcaatt
     1861 agaaaaaatt atacaacaaa acaaataaag acaacatgac aacagcacat ttatatgacg
     1921 tgagcgtaca atggaatgaa gacagaaaag gaacaatgag ctctactgtt ttaacacaaa
     1981 aaatagaagt ggcaacacca ccagaatttg caaaaggaat gcccaatata tggtcgcctg
     2041 agcatttatt tgttgctgcc gtaaacagtt gcttgatgac cacattttta gctattgcag
     2101 agaactcaaa attagagt
//
LOCUS       DAOQQV010000202         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_34005_length_1984_cov_7.653707, whole genome shotgun sequence.
ACCESSION   DAOQQV010000202 DAOQQV010000000
VERSION     DAOQQV010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34005_length_1984_cov_7.653707"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLL59_12490"
     CDS             complement(<1..694)
                     /locus_tag="PLL59_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMY24674.1"
                     /translation="MQTVDSINFNNKRALVRVDFNVPFDKQFNITDDTRIKAALPTIN
                     KILNDGGSVVLTSHLGRPLKKLKDDGTVDIEKFSLKHVVDRLSFLLAKTVLFAPDCID
                     ENSKHLSANLQNGQILLLENTRFHKGEEKGDEDFAAQLAYHGDVYVNDAFGSAHRAHA
                     STTIVAKHFAKENKCFGYLMAAEVANANKVLHSAAKPFTAILGGAKVSDKILIIENLL
                     NIADNIIIGGGMA"
     gene            complement(797..1801)
                     /gene="gap"
                     /locus_tag="PLL59_12495"
     CDS             complement(797..1801)
                     /gene="gap"
                     /locus_tag="PLL59_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMY24675.1"
                     /translation="MSKPKIAINGLGRIGRLVFQILLDRKNVDLVVVNDIAPNDALVY
                     LLKHDTAQGKWNKHIEAKENHLFIDGEEVISTSIADSKDLPWKKHEIDIVVECTGRYR
                     TREKLHEHITSGAKKVIISSPADETVKTIVLGINDAAVLPEDTILSNASCTTNCLAPM
                     VKILHEHFGIKQGVMNTVHAYTSDQKLHDSIHGSDFRRARAAAQNIVPTSSNANKALE
                     WVMPEIKGKIKGGAVRVPIIVGSLTELYCNLEQAPSIEEINTVFKTAAHGKWKNILEY
                     TEEPIVSSDIIHNPNSCIFDAALTQKSGDMYKIVGWYDNEYGYANRLAELVEKVAFLA
                     "
     gene            1912..1983
                     /locus_tag="PLL59_12500"
     tRNA            1912..1983
                     /locus_tag="PLL59_12500"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1945..1947,aa:Glu,seq:ttc)
BASE COUNT          603 a          369 c          325 g          687 t
ORIGIN      
        1 atgccattcc accaccaata ataatgttat ctgcaatgtt taataagttc tcaataatga
       61 gaattttatc agaaactttc gcaccaccta aaattgctgt aaaaggttta gcagcgctat
      121 gtaaaacttt attggcattt gcgacttctg ctgccatcaa gtatccaaag catttatttt
      181 cttttgcaaa atgttttgca acaattgttg tgcttgcatg tgcgcgatgt gctgagccaa
      241 atgcatcatt tacatagaca tctccatgat aagcaagctg tgctgcaaaa tcttcatcac
      301 ctttctcttc acctttatga aaacgtgtat tttctaacaa taagatttga ccattttgta
      361 aattggcaga aagatgttta ctattttcat caatacaatc tggcgcaaac aatactgttt
      421 ttgccaacaa aaatgacagc ctatcaacga catgttttaa tgaaaatttt tctatatcaa
      481 cagtgccgtc gtctttcaat ttttttaatg gcctgcccaa atgagacgtc agcacaacac
      541 ttccgccatc attcaatatt ttattgatag ttggcaaggc agctttgatg cgcgtgtcat
      601 cagtaatatt aaattgttta tcgaaaggca cattaaaatc tacacgtacg agcgcgcgtt
      661 tgttattaaa attaattgaa tctacagttt gcataagagt tataattttt aagtaatttt
      721 tagcgttatt gttgtaaaat taaataataa acacgcaata aacattgaat atttaggttg
      781 aatttatttt taaatattaa gcaagaaatg cgactttttc caccaattca gctaagcgat
      841 tagcataacc atattcatta tcataccagc ctacaatttt atacatgtcg ccactttttt
      901 gagtaagtgc cgcatcaaaa atacaagaat ttggattatg aataatatcg cttgacacaa
      961 ttggttcttc ggtatattct aaaatatttt tccattttcc atgagctgct gttttaaaca
     1021 cagtattgat ttcttctata cttggcgctt gttctaaatt gcaatataat tctgtcaaag
     1081 aaccaacaat aataggcaca cgcacagcac cacctttaat tttaccttta atttctggca
     1141 tcacccattc gagtgctttg ttggcattag aagaagtagg tactatattt tgtgcagcag
     1201 ctctagcccg tctgaagtcg cttccatgaa tgctatcgtg taatttttgg tcactagtat
     1261 aagcatgtac cgtgttcatc acaccttgtt taataccaaa atgctcatga agaattttca
     1321 ccattggtgc taagcaattt gtcgtgcatg aggcattaga caaaatagta tcttcaggca
     1381 acacagcagc atcattaatg cctaaaacta ttgtttttac tgtctcgtcg gcaggtgaag
     1441 agatgattac tttttttgca ccacttgtga tatgttcgtg taatttttct cgcgtacgat
     1501 atcggccagt acactcgaca acgatatcaa tttcgtgttt tttccaaggt aaatcttttg
     1561 agtcagctat tgatgtgctg ataacttctt ccccatctat aaaaaggtga ttttctttag
     1621 cttcaatatg tttattccat ttaccttgcg ctgtatcatg ttttaataaa taaaccaaag
     1681 cgtcatttgg cgcaatatca ttgacaacta ctaaatcgac atttttgcgg tctaataata
     1741 tttgaaaaac aaggcggcca attctgccga ggccatttat agctatttta ggtttactca
     1801 tattgtttgt ttgttgtaaa agtagtagaa aatacgatta tcaaaaagaa attattgtta
     1861 aggagttgca gaaataaaaa atgttagtat atttgcactc gcttttagaa cggcccgttc
     1921 gtctaggggt taggacagga gattttcatt cttcaaacag gggttcgatt cccctacggg
     1981 ctac
//
LOCUS       DAOQQV010000203         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_34034_length_1983_cov_7.795643, whole genome shotgun sequence.
ACCESSION   DAOQQV010000203 DAOQQV010000000
VERSION     DAOQQV010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34034_length_1983_cov_7.795643"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PLL59_12505"
     CDS             <1..1120
                     /locus_tag="PLL59_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMY24676.1"
                     /translation="SGHGNIETAVEAVKKGAFDYIPKPPDLNRLLITVRNAMDKVSLV
                     TETKILKKKIAKSKEIVGESNPIVKIKETIAKVAPTEARVLITGDNGAGKELVARWLH
                     EQSNRAAGPLVEVNCAAIPSELIESELFGHEKGAFTSAIKQRIGKFEQANGGTLFLDE
                     IGDMSLSAQAKVLRALQENKITKVGGDKEEKVDVRVVAATNKDLLKEVEENRFRMDLY
                     HRLSVILIHVPSLNERKDDIPVLVDHFLQEICGEYGIPVKNIEKDAIETLQSRNWTGN
                     IRELRNVVERLVIMSEKVITKQDVIDYSGPNTKAENKGIDFDKFANLQQLNDYVERSF
                     IENRLDKNSWNTSKTADDIGLSKKALEEKLESLGLKKR"
     gene            complement(1176..>1983)
                     /locus_tag="PLL59_12510"
     CDS             complement(1176..>1983)
                     /locus_tag="PLL59_12510"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HMY24677.1"
                     /translation="DCGEGTQERLHENKIKWFKIDYIFISHLHGDHYFGLIGLLTTFN
                     LLKRISPLTIFAPKDLKKIISMQLKASNTSLNYLIKFVSTLDDEKRLLLDLPECEVYS
                     FPLKHKIPTTGFLFVEKAEPRLLDLQKLEKYNIAKSDYKLLKQGFDVKDKNGKKIKNS
                     LLTTKGKPPRSYAFCSDTMYNLPMVKIIKNVDLLYHEATFLNEHLQRANETKHSTALQ
                     AAQIAKKANAKKLLLGHFSSRYLDLSVLQDEAQTIFKNVELAIEGEVFKV"
BASE COUNT          696 a          322 c          367 g          598 t
ORIGIN      
        1 atcagggcac ggcaatattg aaacggctgt agaagcagta aaaaaaggtg ctttcgatta
       61 tattccaaaa ccaccagacc tcaaccgttt attaattacg gtacgcaatg ctatggataa
      121 agtttcttta gtaacagaaa caaaaatcct gaaaaagaaa attgcaaaat caaaagaaat
      181 agttggcgaa tctaatccga ttgtaaaaat aaaagaaacg attgcaaaag ttgcgccaac
      241 agaagcacgt gttttgataa ctggtgacaa tggcgctggc aaagaattag tagcacgctg
      301 gctacacgaa caaagcaaca gagctgctgg ccctttagta gaagtcaact gcgctgcaat
      361 tccttcagaa ttaattgaaa gcgaattgtt cggtcatgaa aagggtgcat ttacttccgc
      421 cataaaacaa cgcatcggca aatttgaaca agccaatggt ggaacacttt tccttgatga
      481 aattggtgat atgagtttat cagcgcaagc taaagtattg cgcgctttgc aggaaaataa
      541 aatcacaaaa gttggcggcg acaaagaaga aaaagtagat gtgcgcgtgg tggctgccac
      601 caataaagat ttattgaaag aagtagaaga aaatcgattc cgtatggact tgtatcatcg
      661 attaagtgtg atattaattc atgtgccgtc attgaacgaa cgtaaagatg acatacccgt
      721 tttagtagac catttcttac aggaaatatg tggtgaatat ggcattcccg ttaaaaacat
      781 agaaaaagat gctatagaaa cattgcaatc acgcaactgg actggaaata tacgcgagct
      841 tagaaacgtt gtagagcgct tggttattat gagtgaaaaa gtaattacga aacaagatgt
      901 gatagattat tctggaccaa atactaaagc tgaaaataaa ggaattgact ttgataaatt
      961 tgccaactta caacagttga atgattatgt agaacgtagt tttatcgaaa atagattaga
     1021 caaaaacagc tggaatactt caaaaacggc tgatgatatc ggactcagca aaaaagcact
     1081 cgaagaaaaa ttggaaagtt taggtttgaa gaaaaggtaa aataataatg tgtatttatc
     1141 atttacatta gtcagataaa tacgcaccat ccttatcata ctttaaacac ttctccttca
     1201 attgctagct ccacattttt aaaaatggtc tgcgcttcat cttgtaaaac agaaagatcc
     1261 aagtagcgag aagaaaaatg tcctaacaat aatttctttg catttgcttt cttagcaatc
     1321 tgagcagcct gtaatgccgt cgaatgtttt gtttcgtttg cacgttgtaa atgctcattt
     1381 aaaaaggtag cttcatgata caataaatct acgtttttaa taatttttac catcggtaaa
     1441 ttgtacatcg tatctgagca aaatgcgtag gaacgtggtg gttttccttt cgtggtaagc
     1501 aaactgtttt ttatcttttt accattttta tcttttacgt caaaaccttg tttcagcaat
     1561 ttataatctg attttgcaat attatatttt tctaatttct gcaaatccaa taagcgtggc
     1621 tcagcttttt cgacaaataa aaatcctgtt gttggtattt tatgttttaa aggaaaggag
     1681 tacacttcac attccggcaa atctaaaagc aatctttttt catcatctaa tgtagaaaca
     1741 aatttgataa gataattcaa agaagtatta cttgctttta attgcatact gatgattttt
     1801 ttcaaatctt ttggtgcaaa aatagtaagt ggtgatatac gttttaatag attaaatgta
     1861 gtgagcagtc ctattaaacc aaaataatga tcgccgtgca gatgcgaaat aaaaatgtaa
     1921 tcgattttaa accatttgat tttattttca tgcaatcgct cttgtgtgcc ttcaccacaa
     1981 tcg
//
LOCUS       DAOQQV010000204         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_37603_length_1874_cov_5.945574, whole genome shotgun sequence.
ACCESSION   DAOQQV010000204 DAOQQV010000000
VERSION     DAOQQV010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37603_length_1874_cov_5.945574"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PLL59_12515"
     CDS             <1..1047
                     /locus_tag="PLL59_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMY24678.1"
                     /translation="TYSRLGNPTVRFVADKIAALEAFGLDDISVFGLLFSSGMAAISA
                     GILSFVKAGDKIITQGNLYGTTNELMVSFLKDYGIETIYADFTDLNFVEDAIINDEKI
                     KLMYIETPANPTLACYNIDGLAAIAQNLGVKTIVDNTFATPYVQQPLKYGIDMVVHSS
                     TKFLNGHGTATTGVLIGVEDGELFKRAYGIQKMIGAVCSPFDAFLLNNGVKTLALRME
                     QHQKNAMHLARFLSQRPEVEKVNYLGLETHENHELAKAQMNGFTGMLSFELKGGLDAG
                     MNFMRGITFCTLTATLGTADTLVTHSASTSHVNVPREQRYQYGITDGLIRVSVGLENI
                     EDVIADFENAFKNI"
     gene            complement(1064..>1874)
                     /locus_tag="PLL59_12520"
     CDS             complement(1064..>1874)
                     /locus_tag="PLL59_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HMY24679.1"
                     /translation="EDFMLLANKAVATYIGKERNKNGKIPFVYRVHDLPNEEKLIDFQ
                     LFAQKFGYKIQFDNPKQIARELNRLMKEIEGKPEQAVLQQLAIRCMAKAIYTSNNIGH
                     YGLGFEYYTHFTSPIRRYPDVMVHRLLDKILNDKKLPTVDEIEYQCKHSSERERAAAD
                     AERASVKYKMAEFMLDRIGEHFEGVISGVKNWGIYVELPQYNCEGLVRTETMRDDIYI
                     FDERKMRMIGKRYDKTYQLGDAVEVKLTAVDIEKKTIDFELVTSKSNKLLI"
BASE COUNT          584 a          351 c          314 g          625 t
ORIGIN      
        1 acctattctc gcttaggcaa tccaactgta agatttgtgg ccgataaaat tgctgcatta
       61 gaagcatttg gattggacga tatatctgtt tttggcttgt tattttcatc aggtatggct
      121 gccatttctg caggaattct atcgtttgta aaagctggcg ataaaatcat tacacaaggt
      181 aatttatatg gcacaacaaa cgagctcatg gtttcttttt tgaaagatta tggcatcgaa
      241 acaatttatg ccgattttac agatttaaat tttgttgaag atgcaatcat caacgatgaa
      301 aaaattaaat taatgtatat agaaacacca gcaaatccta cattagcttg ttataatatt
      361 gatggcttag cagcaatcgc tcaaaattta ggcgtcaaaa ctattgtcga taatacattt
      421 gccacgccat acgtacaaca accattaaaa tatggaattg acatggtggt acattcttcc
      481 accaaatttc taaatggaca tggcacagct actacaggcg tgttaattgg tgtggaagat
      541 ggagaattat ttaaaagagc ctacggaatt caaaaaatga ttggtgctgt ttgctcacct
      601 ttcgatgcat ttttattaaa taatggcgtt aaaacattgg cattacgcat ggagcaacat
      661 caaaaaaatg ctatgcattt agctcgtttt ttaagccagc gacctgaagt agaaaaagtt
      721 aattacttag gactggaaac acatgagaat cacgaattgg caaaagcaca aatgaatggt
      781 tttacaggaa tgcttagctt tgagctcaaa ggtggtttag acgcaggtat gaattttatg
      841 cgaggcatta cattttgcac actcacagca acacttggca ctgctgatac tttggtgaca
      901 cactctgcat caacaagtca tgtaaatgta ccgcgagaac aaagatatca atatggcatt
      961 actgacggtt taatacgtgt ttcagtcggc ttggaaaaca tcgaagatgt tatcgcagat
     1021 ttcgaaaatg cgtttaaaaa tatataagct aatatctgtc aacttatatc aacaacttat
     1081 tgctttttga agttaccaac tcaaagtcta ttgttttctt ttcaatgtca acagctgtaa
     1141 gttttacttc aacagcatcg ccaagctgat atgttttgtc gtatcgcttg cctatcattc
     1201 tcatttttcg ttcatcaaaa atatagatat catcgcgcat cgtttctgtg cgtactaatc
     1261 cttcgcaatt gtattgtggc aactctacat aaattcccca attttttacg cctgaaatca
     1321 caccttcaaa atgttcacca attctatcca acataaactc cgccatttta tatttaacgg
     1381 atgcacgttc tgcatcagcc gcagcgcgtt cgcgttcgct actgtgttta cattgatatt
     1441 caatttcatc aacagtaggt aattttttat catttaaaat cttgtctaat aaacgatgca
     1501 ccattacatc aggatatcgt cgaattggcg aagtaaaatg tgtataatat tcaaaaccta
     1561 aaccataatg tccgatatta ttgcttgtat atatggcttt tgccatgcat cggattgcta
     1621 attgttgcag cacagcttgt tctggctttc cttcaatttc tttcattaaa cgattcagtt
     1681 cccgtgcaat ttgctttgga ttatcaaatt gaattttata tccaaatttt tgtgcaaata
     1741 attgaaaatc gattaacttt tcttcattcg gcaaatcatg tacacgatat acaaatggaa
     1801 tttttccatt tttattacgc tcttttccaa tatacgtcgc aactgcttta tttgccagca
     1861 acataaaatc ttca
//
LOCUS       DAOQQV010000205         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_39146_length_1832_cov_8.019133, whole genome shotgun sequence.
ACCESSION   DAOQQV010000205 DAOQQV010000000
VERSION     DAOQQV010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39146_length_1832_cov_8.019133"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..706)
                     /locus_tag="PLL59_12525"
     CDS             complement(167..706)
                     /locus_tag="PLL59_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMY24680.1"
                     /translation="MLPIQKYCSFCGNEVVYKFAEGRNRVVCEHCNTIHYTNPKIVVG
                     ALSVWEDKVLLCRRAIEPRKGFWNLPAGYLEDFEKAEDGAIRETWEEAGTDIEIQQVL
                     AVYNIPQANQVYIHFYAKLKDGIVRNGEESIESALFSEDEIPWNEMAFTSSAFALRHY
                     FEARKNGNIQTYLGTYPEK"
     gene            complement(713..>1832)
                     /locus_tag="PLL59_12530"
     CDS             complement(713..>1832)
                     /locus_tag="PLL59_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24681.1"
                     /translation="VVNATVFWQGFYHDWSYNHRVNRIGDWIDNVSNKDGSMRAVFTH
                     TAASGSGADVLNYLTFFTYLKTEKAQFFSAATTATIRGREATTTTKVIAVKGKWPSSL
                     AHYKMGTVVLNGFDLYSSNSNNGKVMGSGNADKLSKLFMEVNNFKIQDSTFEFDLNVM
                     LGADCDSPECLNFTPGDNEWFDYQLKVAYQIIAYQDDVHVSNAKLQQDYTWRKPFKTR
                     PDIDPNEIFRKDKVLENQVLKGQKDYHVGIPLISKIDINLPKGKGGVIRKRMETPHLL
                     ALDIAINDYEYNPATGNCVYTADLFFKNWKPNMHILSFGNDGRAIINLGIKFLQIRDP
                     NAVVESQFVKGDIKWQTTQLDQRLANDPHSQKSFVLQK"
BASE COUNT          565 a          361 c          289 g          617 t
ORIGIN      
        1 actgcaatga ttggcgtaaa attaaaaaga gaaaatgcat ttgaaacaat tctgaaagca
       61 actgtgctca gtattatgcc tactatcaac caaaagttat tattgttatt cgttttcata
      121 aaacaaagat atacagaatc gataaattca gcaaagaaaa tagcaattat ttctcaggat
      181 atgtacctaa gtaagtttgg atatttccat tttttctagc ttcaaaataa tggcgcaatg
      241 cgaaagctga cgaggtaaat gccatttcat tccaaggaat ttcatcttca ctaaacaatg
      301 cagattcaat gctttcttca ccattgcgta caattccatc ttttagcttt gcatagaaat
      361 gtatatacac ttgattggct tgcggaatat tataaacagc caaaacctgt tgaatttcaa
      421 tatctgtgcc tgcttcttcc caagtttcgc ggatagcacc atcttcagct ttttcaaaat
      481 cttctaaata acctgctggc aaattccaaa atcctttgcg cggttctatg gctcttcggc
      541 aaagcaatac tttatcttcc caaactgaca atgcaccgac aacaattttt gggttggtgt
      601 aatgtatggt attgcaatgc tcacatacta cgcgatttct gccttctgca aatttataaa
      661 cgacttcatt tccacaaaaa ctacaatatt tctgaatcgg caacatttta gattattttt
      721 gcagtacaaa agacttttgt gaatgtggat cattggctaa tcgctgatct aattgtgttg
      781 tttgccattt gatatcgcct ttaacgaatt ggctttcaac aacagcattt ggatcgcgaa
      841 tctgtaagaa tttaatacca agattgatga tggctctacc atcatttcca aacgataata
      901 tatgcatgtt tggtttccaa tttttgaaaa acaaatcagc cgtataaaca caattacctg
      961 tagcaggatt atattcataa tcattgatgg caatatctaa tgctaacaga tgtggtgttt
     1021 ccatgcgttt acgaataaca cctcctttac cttttggtag attgatatct attttagaaa
     1081 ttaatggtat gccgacatga taatcttttt gcccttttaa aacttgattt tccaacactt
     1141 tatcttttct gaaaatctca tttggatcta tgtcaggcct tgttttgaaa ggcttacgcc
     1201 aagtataatc ttgttgtaat ttagcattgc ttacatgcac atcatcttga taagctataa
     1261 tttgataagc aactttcaac tgataatcaa accactcatt gtcgccaggt gtaaaattca
     1321 aacattctgg cgaatcgcag tcggcaccta acatgacgtt taaatcaaat tcgaatgtgc
     1381 tatcttgaat tttaaaatta ttgacttcca taaataattt cgataattta tctgcattgc
     1441 cagaacccat taccttgcca ttgttgctat tgctactata taaatcaaaa ccatttaaaa
     1501 ccactgtgcc cattttatag tgcgctaagc ttgatggcca tttcccttta actgcgatta
     1561 cttttgttgt tgtagttgct tctcttccac gaattgttgc agtcgttgca gcagaaaaaa
     1621 attgggcttt ctctgttttt agataagtaa aaaaagtgag ataattcagc acatcagcgc
     1681 cactgccaga cgctgcagtg tgagtgaata ccgcacgcat gcttccatct ttgtttgaca
     1741 cattgtctat ccagtcgcct attctattga cacgatgatt ataagaccaa tcgtgataaa
     1801 agccttgcca aaaaacagta gcattaacta cg
//
LOCUS       DAOQQV010000206         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_39426_length_1825_cov_6.006780, whole genome shotgun sequence.
ACCESSION   DAOQQV010000206 DAOQQV010000000
VERSION     DAOQQV010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39426_length_1825_cov_6.006780"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLL59_12535"
     CDS             complement(<1..571)
                     /locus_tag="PLL59_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMY24682.1"
                     /translation="MKYISKIIFLFLFLSIVAESKAQFVMPTLPSIQPGIPANINAQD
                     PTVKEIFKKIEDTLQIRQGEIKVEEVKKSQDIAKQMTPRSKDADEQAGAIGNATNATE
                     VKDNKSSPDKLLKEAQKASETVEKITATATRELREKLDTVDIYGFTYFRQKDVKIFNN
                     AVDIKPPSNYILGVGDQLVVSIWGYADYNR"
     gene            complement(573..1625)
                     /gene="rfbB"
                     /locus_tag="PLL59_12540"
     CDS             complement(573..1625)
                     /gene="rfbB"
                     /locus_tag="PLL59_12540"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HMY24683.1"
                     /translation="MKKILITGGAGFIGSHVVRLFVQKYPQYTIVNLDKLTYAGNLEN
                     LKDIENNTNYIFEKVDIVDANAIDTLFEKYKFDSVIHLAAESHVDRSILNPLEFIMTN
                     VVGTVNLLNAAKKCWHDDFSGKLFYHVSTDEVYGSLGETGLFTEETAYDPRSPYSASK
                     ASSDHFVRAYENTYHLPVVISNCSNNYGAFQFPEKLIPLFINNIKNNKPLPVYGKGDY
                     TRDWLFVEDHATAIDTIFHNGKLGETYNIGGFNEWQNIDLIKLLCAIMDKKLNRINGS
                     SEQLITYVKDRPGHDKRYAIDASKLNKELGWKPSLQFEEGLEKTVDWYLQNETWLQHV
                     TSGEYQNYYKHQYELS"
     gene            complement(1625..>1825)
                     /locus_tag="PLL59_12545"
     CDS             complement(1625..>1825)
                     /locus_tag="PLL59_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMY24684.1"
                     /translation="VEEEYGVSLQADASGKYDVILVAVSHDAYKNLSEDYFTSITNED
                     AVLVDIKNMYKSKISKLEYWTL"
BASE COUNT          545 a          339 c          290 g          651 t
ORIGIN      
        1 agcgattgta atcggcataa ccccaaatag aaacgaccaa ttggtcgcca acacctaaga
       61 tataattgct aggtggtttt atatctactg cgttgttaaa tattttcaca tctttttgac
      121 ggaaataggt aaagccataa atatcaaccg tatctaattt ctcacgaagt tcgcgtgtag
      181 cagtagcagt gattttttca actgtttcag atgctttttg tgcttctttt aatagtttgt
      241 ctggcgagct tttattatct tttacttctg tcgcattagt agcattacca atcgcaccag
      301 cttgttcgtc agcatcttta gaacgaggtg tcatttgttt cgcgatgtct tgcgattttt
      361 taacctcttc tacttttatc tcaccttgac gaatttgtaa agtatcttca atttttttaa
      421 aaatctcttt tacagtcgga tcttgcgcat tgatatttgc aggaattcct ggttgtatag
      481 atggcaaagt cggcatcaca aattgtgctt tcgactcagc cacaatgcta aggaaaagaa
      541 aaaggaaaat tatttttgat atatatttca tactagctta attcatactg atgtttgtaa
      601 taattttgat attcacctga tgtaacgtgt tgtaaccatg tttcattttg caaataccaa
      661 tctactgttt tttccaagcc ttcttcaaac tgcaatgatg gtttccagcc tagttcttta
      721 ttgagttttg acgcgtcaat ggcataacgc ttatcgtgtc ctggtctatc tttcacataa
      781 gtaatcaatt gctcggagct gccatttatt ctatttaatt ttttatccat aatggcacaa
      841 agcaatttta tcaagtcgat attttgccat tcattaaagc caccgatatt gtaagtttcg
      901 ccaagcttgc cattgtgaaa aattgtatca atagcagttg catggtcttc tacaaaaagc
      961 caatctctgg tataatcacc tttgccatat acaggcaatg gtttattgtt tttgatatta
     1021 ttgataaaaa gtggaatcaa tttttctgga aattgaaaag caccataatt gttggagcaa
     1081 ttagaaatga caacaggaag atgataggta ttttcataag ctctcacaaa gtgatctgag
     1141 cttgctttag atgcggaata tggcgaacgt ggatcataag cagtttcttc tgtaaagagt
     1201 cctgtttcac ctaagctgcc atacacttca tccgttgaaa cgtgataaaa taatttgcca
     1261 gaaaaatcat catgccagca tttttttgca gcattcagca aattgaccgt cccgacaaca
     1321 ttagtcatga taaattcgag cggatttaaa atagagcgat caacatgcga ttctgctgct
     1381 aaatgtataa cactatcaaa tttatatttt tcaaataaag tatctatagc attcgcatca
     1441 acgatatcta ctttttcaaa aatataattt gtattatttt cgatgtcttt caagttttct
     1501 aaattaccag cataggttag cttgtccaga ttcacaattg tgtattgcgg atatttctga
     1561 acgaataatc tcaccacgtg tgagccaata aaaccagcgc ctccagtaat taagattttt
     1621 ttcattataa tgtccagtat tctaatttag aaatttttga cttatacata tttttgatat
     1681 ccacgagcac agcatcttca tttgtaatgc ttgtaaaata atcttctgat aaatttttat
     1741 aagcatcatg agaaactgca actaaaatca catcatattt tccacttgcg tcagcttgca
     1801 aacttacacc atattcttct tccac
//
LOCUS       DAOQQV010000207         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_41632_length_1771_cov_6.832751, whole genome shotgun sequence.
ACCESSION   DAOQQV010000207 DAOQQV010000000
VERSION     DAOQQV010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41632_length_1771_cov_6.832751"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1660
                     /locus_tag="PLL59_12550"
     CDS             56..1660
                     /locus_tag="PLL59_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMY24685.1"
                     /translation="MNFNKAFYPFIFSVILAFGVFLGFKIQGNSNAKSKSFYNAAGNS
                     KLEYLLRLIEAKYVDTVDAQSLYDDAIAKMLTDLDPHSVYLPPLEMKHEAEIMEGNFE
                     GIGIEFSIQNDTIQVVTPISGGPSEQVGISAGDKIIKINDTLVAGIKISNDMVMKKLK
                     GPKDTKVKVTMLKSGTRKMVNYTIKRDKIPLYSLDAGFMLDKTTGYIKINRFSKTTYA
                     EFYEKLEALTKQGMQKLILDLRQNPGGFMDQATAMADEFIDGSKTIVYTEGRTFPKTY
                     DKAKTPGMFEQGKLAILIDEGSASASEIVSGAVQDWDRGIIIGRRSFGKGLVQQEYDL
                     GDGSSVRLTIAKYFTPSGRSIQRPYDHNVENYYSDISKRYKDGEVFHQDSIKQDKKIT
                     YKTLIKGRTVYGGGGITPDIYIPIDTSMLNPFVNAVYENNLLQEFAYNYYAGNKTNFS
                     TYKSAANFNQQFAVSQELYAQFVQYCMAHGVNKDVAAYTEKSKAFLSNKLKAFLGKQA
                     WKNDGFYEVIVQDDKMVQRARLELSK"
BASE COUNT          644 a          279 c          336 g          512 t
ORIGIN      
        1 catctttaaa tatatttaag gttaatttag taaagaaaaa attacctttg cagatatgaa
       61 ttttaataaa gcattttatc cgtttatttt ttcagttata ttggcttttg gcgtattctt
      121 aggttttaaa atacaaggaa acagcaatgc aaaaagcaaa tcattttata acgctgccgg
      181 caattcaaaa ttagaatatt tgttacgact tatcgaagct aaatatgtgg acactgttga
      241 cgcacaaagt ttatatgacg atgcgatagc aaaaatgctt actgacctcg acccacatag
      301 cgtgtatttg cctcctttag aaatgaagca tgaagctgaa ataatggaag gcaacttcga
      361 aggtatcggc atcgaatttt ctatacaaaa tgatacgata caagtggtga cgccaattag
      421 tggtggacct tctgagcaag taggaatttc tgctggcgat aaaatcataa aaataaatga
      481 cacattggtt gctggtataa aaattagcaa cgatatggtg atgaaaaaac tgaaaggacc
      541 aaaagacact aaagtaaaag tcaccatgct gaaaagcgga acaagaaaaa tggtcaatta
      601 caccatcaag cgtgataaaa ttcctttgta tagcttagat gctggtttta tgttagacaa
      661 aacaactggc tatattaaaa tcaatcgttt ttctaaaact acttatgctg aattttatga
      721 aaaattagaa gccttaacaa aacaaggaat gcaaaaattg attttagatt tacgacaaaa
      781 tccgggtggt tttatggatc aagctacagc aatggccgat gagtttatcg atggcagcaa
      841 aaccattgtt tatacagaag gaaggacatt tccaaaaacg tatgacaaag ccaaaacacc
      901 tggtatgttt gaacaaggaa aattggcgat tttaatagat gaaggctctg cttctgctag
      961 cgaaattgta agcggcgccg tacaagattg ggacagaggt atcatcattg gcagacgctc
     1021 ttttggcaaa ggcttggtac aacaagaata cgatttaggc gatggcagtt ccgtgcgatt
     1081 aacgattgcc aaatacttta cacctagtgg cagaagcatt caaagaccct acgaccataa
     1141 tgtagaaaat tattatagcg acattagcaa acgttataaa gatggtgaag tgttccatca
     1201 agacagcata aaacaagata aaaaaatcac atacaaaaca ttaatcaaag gccgcaccgt
     1261 gtatggtggt ggtggcatta caccagatat ctatatacca attgatacca gtatgttgaa
     1321 tccttttgta aatgctgttt atgaaaataa tttattgcaa gagtttgctt ataattacta
     1381 tgctggcaat aaaactaact ttagcacata caaatcagct gctaacttta atcaacaatt
     1441 tgctgtaagc caggaattat atgcacagtt tgtacagtat tgtatggcac atggcgtaaa
     1501 caaagacgtt gcagcttata ctgaaaaatc taaagcattt ttgagcaata aactcaaagc
     1561 atttttagga aagcaagcat ggaaaaatga tggcttttat gaagttatag ttcaagatga
     1621 caagatggta cagcgtgctc gattagagct aagcaaatag agtagataaa tcatttttaa
     1681 tctgttcgaa tctagtgctg ttcaaatttt tcataagaaa cgcttcatat tcattatgca
     1741 aattaaaagg cgtatttaat ttagtgttta c
//
LOCUS       DAOQQV010000208         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0409
            NODE_46927_length_1657_cov_6.094881, whole genome shotgun sequence.
ACCESSION   DAOQQV010000208 DAOQQV010000000
VERSION     DAOQQV010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810041
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,512
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,477
            CDSs (with protein)               :: 2,477
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46927_length_1657_cov_6.094881"
                     /isolate="AMR_MDS_0409"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLL59_12555"
     CDS             <1..1020
                     /locus_tag="PLL59_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HMY24686.1"
                     /translation="TRGAGQESTYYGKIEDLVEQIKVATPIGTLVSNTFSHDASGINV
                     LPLFVGSEGTLGVVTEISVKIKKLPKNYRWIVALFPDFQSGSQYLQELTQAGVKPSVV
                     RLSDANETNLFSKMSSSEEQPGFVNDLKKEAQKLILQWKNLTAPCVLIMRFPQSNISV
                     SSQVIYAKDLVAKHKGMLAPATIGDKWAENRFKLPYLRDTLVEHAIYIDTMETLVRWK
                     DVQKMHQALTKELNKCAAFHKNKGIFLAHISHVYPNAACMYFTLITPMLKGREIEQWQ
                     DLKDVVTNTILQNNGSISHHHSVGLDHQKWYKKYLDNLAMDLLQSIKNKLDPKGIMNP
                     GKLFS"
     gene            1031..>1657
                     /locus_tag="PLL59_12560"
     CDS             1031..>1657
                     /locus_tag="PLL59_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMY24687.1"
                     /translation="MNNRKTNIQRASSEFFDIVVIGGGITGAGIALMAAKKGWKTLIV
                     DKHDFAFGTSSRSAKMIHGGLRYLQNLQIKLVKEALHEREHLLKEYPHLVKPQPFFMP
                     IYKSKLNKLKLGIGLSGYDYLTGESSMPKHEAIKVDKVKERFPQIKSDNIVGGYIYYD
                     AKTNDARLTNDIIQEAVDLGAVSINYFEVENIICKDNIVNEIICTDNIS"
BASE COUNT          588 a          264 c          299 g          506 t
ORIGIN      
        1 acaagaggcg ctggccaaga atccacttat tatggtaaaa ttgaagattt agtagagcaa
       61 attaaagtag ccacacctat aggaacatta gtaagcaaca ctttttcaca tgacgcttcc
      121 ggaataaatg tattgccatt atttgtaggc agtgaaggca ctttaggcgt tgttacagag
      181 attagcgtta aaataaaaaa actaccgaaa aactacagat ggatagttgc tttatttcca
      241 gattttcagt caggaagtca gtatttacaa gagcttacac aagcaggcgt aaaaccttca
      301 gtagtacgct tgtccgatgc caatgaaacc aacttatttt ctaaaatgtc ttcatcggaa
      361 gagcagcctg gatttgtaaa tgacctaaaa aaagaagctc aaaaattaat tttacaatgg
      421 aaaaatctaa ctgcaccttg cgtgttgatt atgcgttttc cacaatcaaa tattagtgta
      481 tcatcacaag taatttatgc taaagattta gtggcaaaac acaaaggaat gctcgctcct
      541 gctaccattg gtgataagtg ggcagaaaac cgttttaagt taccttatct tcgcgatacc
      601 ttagtagaac atgctattta tattgatact atggagactt tggtgcgatg gaaagatgta
      661 cagaaaatgc atcaggcatt gacaaaagaa ctgaataaat gcgctgcttt tcataaaaat
      721 aaaggcattt ttttagcgca tatttcgcat gtctatccaa atgctgcttg tatgtatttt
      781 acgctaataa cgccaatgtt aaaaggcaga gaaatagagc aatggcaaga cttaaaagat
      841 gttgttacga atactatttt acaaaataac ggctcaattt ctcatcatca cagtgttggc
      901 ttagatcatc aaaaatggta taaaaaatat ctcgataatt tggcaatgga tttattgcaa
      961 tcgataaaaa ataaattaga tccaaaaggc atcatgaatc caggcaaatt gttcagttaa
     1021 aacattgttt atgaacaatc gtaaaactaa tattcaaaga gcttcttcag aattctttga
     1081 tattgttgtc atcggcggtg gaattactgg tgctggtatt gcattaatgg ctgcaaaaaa
     1141 aggctggaaa acattaatcg ttgataaaca tgattttgct tttggcacaa gctcacgttc
     1201 ggcaaaaatg attcacggtg gattacgtta tttgcaaaat ttacaaatca aattagtaaa
     1261 agaagcatta catgaacgcg aacacttact gaaggaatat ccacatttgg taaaaccgca
     1321 accgtttttt atgccaattt ataaatctaa attaaacaaa cttaaattag gtattggcct
     1381 ttctggttac gattatctca ctggtgaaag tagcatgcct aaacatgaag ccattaaagt
     1441 tgataaggtt aaagaacgat ttcctcaaat taaatctgat aacatcgttg gtggttatat
     1501 ctattatgat gcaaagacta atgatgctag gctaaccaac gacatcattc aagaagctgt
     1561 tgacttaggt gctgtttcta ttaattattt tgaagttgaa aatattattt gtaaggataa
     1621 cattgtaaat gaaattattt gcacagataa tatttct
//
